LOCUS       DANJMI010000001       130071 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000001 DANJMI010000000
VERSION     DANJMI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 130071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 130071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..130071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.001"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            212..604
                     /locus_tag="PWU81_000001"
     CDS             212..604
                     /locus_tag="PWU81_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL1108742.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="PWU81_000002"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="PWU81_000002"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL1108743.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="PWU81_000003"
     CDS             3165..4775
                     /locus_tag="PWU81_000003"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDL1108744.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="PWU81_000004"
     CDS             5067..5936
                     /locus_tag="PWU81_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDL1108745.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="PWU81_000005"
     CDS             6101..7372
                     /locus_tag="PWU81_000005"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL1108746.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="PWU81_000006"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="PWU81_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL1108747.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="PWU81_000007"
     CDS             8763..9026
                     /locus_tag="PWU81_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL1108748.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="PWU81_000008"
     CDS             9471..9920
                     /locus_tag="PWU81_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1108749.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="PWU81_000009"
     CDS             9977..11395
                     /locus_tag="PWU81_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1108750.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="PWU81_000010"
     CDS             11395..12255
                     /locus_tag="PWU81_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDL1108751.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="PWU81_000011"
     CDS             12429..13202
                     /locus_tag="PWU81_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL1108752.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="PWU81_000012"
     CDS             13349..13714
                     /locus_tag="PWU81_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HDL1108753.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="PWU81_000013"
     CDS             complement(13862..15070)
                     /locus_tag="PWU81_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1108754.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="PWU81_000014"
     CDS             complement(15089..15367)
                     /locus_tag="PWU81_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108755.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="PWU81_000015"
     CDS             15521..16216
                     /locus_tag="PWU81_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL1108756.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="PWU81_000016"
     CDS             complement(16658..17455)
                     /locus_tag="PWU81_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL1108757.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="PWU81_000017"
     CDS             complement(17452..18636)
                     /locus_tag="PWU81_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL1108758.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="PWU81_000018"
     CDS             complement(18661..19908)
                     /locus_tag="PWU81_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL1108759.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="PWU81_000019"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="PWU81_000019"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL1108760.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="PWU81_000020"
     CDS             21888..22202
                     /locus_tag="PWU81_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL1108761.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="PWU81_000021"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="PWU81_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL1108762.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="PWU81_000022"
     CDS             22887..23531
                     /locus_tag="PWU81_000022"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL1108763.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="PWU81_000023"
     CDS             23546..23875
                     /locus_tag="PWU81_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDL1108764.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="PWU81_000024"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="PWU81_000024"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL1108765.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="PWU81_000025"
     CDS             24863..25687
                     /locus_tag="PWU81_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDL1108766.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="PWU81_000026"
     CDS             25680..26027
                     /locus_tag="PWU81_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDL1108767.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="PWU81_000027"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="PWU81_000027"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL1108768.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="PWU81_000028"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="PWU81_000028"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL1108769.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="PWU81_000029"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="PWU81_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL1108770.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="PWU81_000030"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="PWU81_000030"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL1108771.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="PWU81_000031"
     CDS             31657..32451
                     /locus_tag="PWU81_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1108772.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="PWU81_000032"
     CDS             32441..34483
                     /locus_tag="PWU81_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1108773.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="PWU81_000033"
     CDS             34764..34913
                     /locus_tag="PWU81_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDL1108774.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="PWU81_000034"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="PWU81_000034"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDL1108775.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="PWU81_000035"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="PWU81_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDL1108776.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="PWU81_000036"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="PWU81_000036"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDL1108777.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="PWU81_000037"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="PWU81_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDL1108778.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="PWU81_000038"
     CDS             39330..40412
                     /locus_tag="PWU81_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDL1108779.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="PWU81_000039"
     CDS             40412..41215
                     /locus_tag="PWU81_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL1108780.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="PWU81_000040"
     CDS             41285..42025
                     /locus_tag="PWU81_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL1108781.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="PWU81_000041"
     CDS             42036..43040
                     /locus_tag="PWU81_000041"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDL1108782.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="PWU81_000042"
     CDS             complement(43085..44929)
                     /locus_tag="PWU81_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108783.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45140..45658
                     /locus_tag="PWU81_000043"
     CDS             45140..45658
                     /locus_tag="PWU81_000043"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL1108784.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45786..46469
                     /locus_tag="PWU81_000044"
     CDS             45786..46469
                     /locus_tag="PWU81_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDL1108785.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46778..46851
                     /locus_tag="PWU81_000045"
     tRNA            46778..46851
                     /locus_tag="PWU81_000045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46812..46814,aa:Arg,seq:tct)
     gene            47010..47369
                     /locus_tag="PWU81_000046"
     CDS             47010..47369
                     /locus_tag="PWU81_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HDL1108786.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47492..49123
                     /locus_tag="PWU81_000047"
     CDS             47492..49123
                     /locus_tag="PWU81_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDL1108787.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQFPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49185..49826)
                     /locus_tag="PWU81_000048"
     CDS             complement(49185..49826)
                     /locus_tag="PWU81_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL1108788.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    50012..50088
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            50108..50416
                     /gene="rplU"
                     /locus_tag="PWU81_000049"
     CDS             50108..50416
                     /gene="rplU"
                     /locus_tag="PWU81_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL1108789.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50443..50787
                     /locus_tag="PWU81_000050"
     CDS             50443..50787
                     /locus_tag="PWU81_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDL1108790.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50800..51093
                     /gene="rpmA"
                     /locus_tag="PWU81_000051"
     CDS             50800..51093
                     /gene="rpmA"
                     /locus_tag="PWU81_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL1108791.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51527..54334
                     /locus_tag="PWU81_000052"
     CDS             51527..54334
                     /locus_tag="PWU81_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL1108792.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            54525..55016
                     /locus_tag="PWU81_000053"
     CDS             54525..55016
                     /locus_tag="PWU81_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1108793.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55119..56108
                     /gene="manX"
                     /locus_tag="PWU81_000054"
     CDS             55119..56108
                     /gene="manX"
                     /locus_tag="PWU81_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL1108794.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56141..56944
                     /locus_tag="PWU81_000055"
     CDS             56141..56944
                     /locus_tag="PWU81_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL1108795.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56965..57879
                     /locus_tag="PWU81_000056"
     CDS             56965..57879
                     /locus_tag="PWU81_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDL1108796.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57962..58336
                     /locus_tag="PWU81_000057"
     CDS             57962..58336
                     /locus_tag="PWU81_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HDL1108797.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58475..58720
                     /locus_tag="PWU81_000058"
     CDS             58475..58720
                     /locus_tag="PWU81_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL1108798.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            58892..59956
                     /locus_tag="PWU81_000059"
     CDS             58892..59956
                     /locus_tag="PWU81_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL1108799.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            60202..60627
                     /locus_tag="PWU81_000060"
     CDS             60202..60627
                     /locus_tag="PWU81_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL1108800.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            60620..61069
                     /gene="nusB"
                     /locus_tag="PWU81_000061"
     CDS             60620..61069
                     /gene="nusB"
                     /locus_tag="PWU81_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL1108801.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            61166..62020
                     /locus_tag="PWU81_000062"
     CDS             61166..62020
                     /locus_tag="PWU81_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HDL1108802.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            62021..63358
                     /locus_tag="PWU81_000063"
     CDS             62021..63358
                     /locus_tag="PWU81_000063"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL1108803.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            63363..63587
                     /locus_tag="PWU81_000064"
     CDS             63363..63587
                     /locus_tag="PWU81_000064"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL1108804.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            63589..64479
                     /locus_tag="PWU81_000065"
     CDS             63589..64479
                     /locus_tag="PWU81_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL1108805.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            64488..65306
                     /locus_tag="PWU81_000066"
     CDS             64488..65306
                     /locus_tag="PWU81_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDL1108806.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            65413..65862
                     /locus_tag="PWU81_000067"
     CDS             65413..65862
                     /locus_tag="PWU81_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL1108807.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            65880..67562
                     /gene="recN"
                     /locus_tag="PWU81_000068"
     CDS             65880..67562
                     /gene="recN"
                     /locus_tag="PWU81_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL1108808.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(67617..67733)
                     /locus_tag="PWU81_000069"
     CDS             complement(67617..67733)
                     /locus_tag="PWU81_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HDL1108809.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(67806..68762)
                     /locus_tag="PWU81_000070"
     CDS             complement(67806..68762)
                     /locus_tag="PWU81_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL1108810.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            68933..69301
                     /locus_tag="PWU81_000071"
     CDS             68933..69301
                     /locus_tag="PWU81_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HDL1108811.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            69471..69902
                     /gene="mraZ"
                     /locus_tag="PWU81_000072"
     CDS             69471..69902
                     /gene="mraZ"
                     /locus_tag="PWU81_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL1108812.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            69923..70882
                     /gene="rsmH"
                     /locus_tag="PWU81_000073"
     CDS             69923..70882
                     /gene="rsmH"
                     /locus_tag="PWU81_000073"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL1108813.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            70887..71288
                     /gene="ftsL"
                     /locus_tag="PWU81_000074"
     CDS             70887..71288
                     /gene="ftsL"
                     /locus_tag="PWU81_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL1108814.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            71289..73481
                     /locus_tag="PWU81_000075"
     CDS             71289..73481
                     /locus_tag="PWU81_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL1108815.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            73507..74469
                     /locus_tag="PWU81_000076"
     CDS             73507..74469
                     /locus_tag="PWU81_000076"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL1108816.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            74483..75844
                     /locus_tag="PWU81_000077"
     CDS             74483..75844
                     /locus_tag="PWU81_000077"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL1108817.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            75870..76958
                     /gene="murG"
                     /locus_tag="PWU81_000078"
     CDS             75870..76958
                     /gene="murG"
                     /locus_tag="PWU81_000078"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL1108818.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            76973..78193
                     /locus_tag="PWU81_000079"
     CDS             76973..78193
                     /locus_tag="PWU81_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL1108819.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            78349..79674
                     /gene="ftsA"
                     /locus_tag="PWU81_000080"
     CDS             78349..79674
                     /gene="ftsA"
                     /locus_tag="PWU81_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL1108820.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            79697..80938
                     /gene="ftsZ"
                     /locus_tag="PWU81_000081"
     CDS             79697..80938
                     /gene="ftsZ"
                     /locus_tag="PWU81_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL1108821.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            80955..81632
                     /locus_tag="PWU81_000082"
     CDS             80955..81632
                     /locus_tag="PWU81_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL1108822.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            81646..82245
                     /locus_tag="PWU81_000083"
     CDS             81646..82245
                     /locus_tag="PWU81_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDL1108823.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            82260..82547
                     /locus_tag="PWU81_000084"
     CDS             82260..82547
                     /locus_tag="PWU81_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL1108824.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            82596..83378
                     /locus_tag="PWU81_000085"
     CDS             82596..83378
                     /locus_tag="PWU81_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDL1108825.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            83399..84106
                     /locus_tag="PWU81_000086"
     CDS             83399..84106
                     /locus_tag="PWU81_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDL1108826.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            84368..87166
                     /gene="ileS"
                     /locus_tag="PWU81_000087"
     CDS             84368..87166
                     /gene="ileS"
                     /locus_tag="PWU81_000087"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL1108827.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(87215..88741)
                     /locus_tag="PWU81_000088"
     CDS             complement(87215..88741)
                     /locus_tag="PWU81_000088"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL1108828.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(88756..89403)
                     /locus_tag="PWU81_000089"
     CDS             complement(88756..89403)
                     /locus_tag="PWU81_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDL1108829.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(89587..89916)
                     /locus_tag="PWU81_000090"
     CDS             complement(89587..89916)
                     /locus_tag="PWU81_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HDL1108830.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            90093..90821
                     /gene="sfsA"
                     /locus_tag="PWU81_000091"
     CDS             90093..90821
                     /gene="sfsA"
                     /locus_tag="PWU81_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL1108831.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            90837..91850
                     /locus_tag="PWU81_000092"
     CDS             90837..91850
                     /locus_tag="PWU81_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL1108832.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            91850..93127
                     /locus_tag="PWU81_000093"
     CDS             91850..93127
                     /locus_tag="PWU81_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL1108833.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            93190..95892
                     /locus_tag="PWU81_000094"
     CDS             93190..95892
                     /locus_tag="PWU81_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL1108834.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            96044..96361
                     /locus_tag="PWU81_000095"
     CDS             96044..96361
                     /locus_tag="PWU81_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1108835.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            96391..96711
                     /locus_tag="PWU81_000096"
     CDS             96391..96711
                     /locus_tag="PWU81_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL1108836.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            96819..98279
                     /locus_tag="PWU81_000097"
     CDS             96819..98279
                     /locus_tag="PWU81_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1108837.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            98347..98568
                     /locus_tag="PWU81_000098"
     CDS             98347..98568
                     /locus_tag="PWU81_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108838.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            98599..98781
                     /locus_tag="PWU81_000099"
     CDS             98599..98781
                     /locus_tag="PWU81_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HDL1108839.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            98781..99194
                     /locus_tag="PWU81_000100"
     CDS             98781..99194
                     /locus_tag="PWU81_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDL1108840.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            99317..100498
                     /locus_tag="PWU81_000101"
     CDS             99317..100498
                     /locus_tag="PWU81_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1108841.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(100569..100733)
                     /locus_tag="PWU81_000102"
     CDS             complement(100569..100733)
                     /locus_tag="PWU81_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108842.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            100887..100960
                     /locus_tag="PWU81_000103"
     tRNA            100887..100960
                     /locus_tag="PWU81_000103"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100921..100923,aa:Arg,seq:cct)
     gene            complement(101029..102168)
                     /locus_tag="PWU81_000104"
     CDS             complement(101029..102168)
                     /locus_tag="PWU81_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1108843.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(102467..103102)
                     /locus_tag="PWU81_000105"
     CDS             complement(102467..103102)
                     /locus_tag="PWU81_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108844.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(103215..103850)
                     /locus_tag="PWU81_000106"
     CDS             complement(103215..103850)
                     /locus_tag="PWU81_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HDL1108845.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(103884..104345)
                     /locus_tag="PWU81_000107"
     CDS             complement(103884..104345)
                     /locus_tag="PWU81_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDL1108846.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(104475..104906)
                     /locus_tag="PWU81_000108"
     CDS             complement(104475..104906)
                     /locus_tag="PWU81_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL1108847.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(104924..105244)
                     /locus_tag="PWU81_000109"
     CDS             complement(104924..105244)
                     /locus_tag="PWU81_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1108848.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            105543..106319
                     /locus_tag="PWU81_000110"
     CDS             105543..106319
                     /locus_tag="PWU81_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDL1108849.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            106334..106537
                     /locus_tag="PWU81_000111"
     CDS             106334..106537
                     /locus_tag="PWU81_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1108850.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            106553..106891
                     /locus_tag="PWU81_000112"
     CDS             106553..106891
                     /locus_tag="PWU81_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HDL1108851.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            106878..107057
                     /locus_tag="PWU81_000113"
     CDS             106878..107057
                     /locus_tag="PWU81_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108852.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(107100..107570)
                     /locus_tag="PWU81_000114"
     CDS             complement(107100..107570)
                     /locus_tag="PWU81_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108853.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            107657..108355
                     /locus_tag="PWU81_000115"
     CDS             107657..108355
                     /locus_tag="PWU81_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDL1108854.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            108533..108874
                     /locus_tag="PWU81_000116"
     CDS             108533..108874
                     /locus_tag="PWU81_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108855.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            108867..109538
                     /locus_tag="PWU81_000117"
     CDS             108867..109538
                     /locus_tag="PWU81_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL1108856.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            109544..110236
                     /locus_tag="PWU81_000118"
     CDS             109544..110236
                     /locus_tag="PWU81_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108857.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            110233..110982
                     /locus_tag="PWU81_000119"
     CDS             110233..110982
                     /locus_tag="PWU81_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1108858.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            110994..111263
                     /locus_tag="PWU81_000120"
     CDS             110994..111263
                     /locus_tag="PWU81_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108859.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            111269..111433
                     /locus_tag="PWU81_000121"
     CDS             111269..111433
                     /locus_tag="PWU81_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108860.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            111426..111725
                     /locus_tag="PWU81_000122"
     CDS             111426..111725
                     /locus_tag="PWU81_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108861.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            111725..112027
                     /locus_tag="PWU81_000123"
     CDS             111725..112027
                     /locus_tag="PWU81_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL1108862.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            112067..112432
                     /locus_tag="PWU81_000124"
     CDS             112067..112432
                     /locus_tag="PWU81_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108863.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            112922..113062
                     /locus_tag="PWU81_000125"
     CDS             112922..113062
                     /locus_tag="PWU81_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108864.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            113059..113241
                     /locus_tag="PWU81_000126"
     CDS             113059..113241
                     /locus_tag="PWU81_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108865.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            113254..113448
                     /locus_tag="PWU81_000127"
     CDS             113254..113448
                     /locus_tag="PWU81_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108866.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(113481..113693)
                     /locus_tag="PWU81_000128"
     CDS             complement(113481..113693)
                     /locus_tag="PWU81_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108867.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            113818..114021
                     /locus_tag="PWU81_000129"
     CDS             113818..114021
                     /locus_tag="PWU81_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108868.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            114018..114290
                     /locus_tag="PWU81_000130"
     CDS             114018..114290
                     /locus_tag="PWU81_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HDL1108869.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            114291..114476
                     /locus_tag="PWU81_000131"
     CDS             114291..114476
                     /locus_tag="PWU81_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108870.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            114463..114810
                     /locus_tag="PWU81_000132"
     CDS             114463..114810
                     /locus_tag="PWU81_000132"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108871.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            114770..115246
                     /locus_tag="PWU81_000133"
     CDS             114770..115246
                     /locus_tag="PWU81_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HDL1108872.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(115377..116105)
                     /locus_tag="PWU81_000134"
     CDS             complement(115377..116105)
                     /locus_tag="PWU81_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HDL1108873.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            116153..116431
                     /locus_tag="PWU81_000135"
     CDS             116153..116431
                     /locus_tag="PWU81_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108874.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            116433..116819
                     /locus_tag="PWU81_000136"
     CDS             116433..116819
                     /locus_tag="PWU81_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108875.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            116816..117196
                     /locus_tag="PWU81_000137"
     CDS             116816..117196
                     /locus_tag="PWU81_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL1108876.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            117308..117760
                     /locus_tag="PWU81_000138"
     CDS             117308..117760
                     /locus_tag="PWU81_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL1108877.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            117757..119484
                     /locus_tag="PWU81_000139"
     CDS             117757..119484
                     /locus_tag="PWU81_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL1108878.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            119548..120735
                     /locus_tag="PWU81_000140"
     CDS             119548..120735
                     /locus_tag="PWU81_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL1108879.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            120707..121276
                     /locus_tag="PWU81_000141"
     CDS             120707..121276
                     /locus_tag="PWU81_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HDL1108880.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            121289..122653
                     /locus_tag="PWU81_000142"
     CDS             121289..122653
                     /locus_tag="PWU81_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL1108881.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            122655..122933
                     /locus_tag="PWU81_000143"
     CDS             122655..122933
                     /locus_tag="PWU81_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL1108882.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            122914..123240
                     /locus_tag="PWU81_000144"
     CDS             122914..123240
                     /locus_tag="PWU81_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL1108883.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            123230..123568
                     /locus_tag="PWU81_000145"
     CDS             123230..123568
                     /locus_tag="PWU81_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108884.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            123558..123923
                     /locus_tag="PWU81_000146"
     CDS             123558..123923
                     /locus_tag="PWU81_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL1108885.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            123930..124556
                     /locus_tag="PWU81_000147"
     CDS             123930..124556
                     /locus_tag="PWU81_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1108886.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            124556..125020
                     /locus_tag="PWU81_000148"
     CDS             124556..125020
                     /locus_tag="PWU81_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108887.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            125215..127524
                     /locus_tag="PWU81_000149"
     CDS             125215..127524
                     /locus_tag="PWU81_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL1108888.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            127536..128240
                     /locus_tag="PWU81_000150"
     CDS             127536..128240
                     /locus_tag="PWU81_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1108889.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            128237..>130071
                     /locus_tag="PWU81_000151"
     CDS             128237..>130071
                     /locus_tag="PWU81_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1108890.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
BASE COUNT        43155 a        21838 c        27227 g        37851 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaaaatttat cgttattata tgattaagaa ggattgaata
    45121 aagaaagaag ttgaaaacga tacctgatta caaaaaaata acttttataa gttctataac
    45181 gttgattata tttttaattc ttattcttct cgtcgtcttg gttccccgaa tttttggttt
    45241 gactccttat attattgagg acaaagaaat gagtccatat tatgcacaag gaaatctgat
    45301 atatgtacga ccgaaaaata caaatgagat tttagtcgga gatattatta catattatga
    45361 aaattcagga aaaaaaatct cgacgcgtcg cgtaatagcc gttgaaaaca accacgaaga
    45421 tttttataca aaagctgata caaagactta tattgagcct ggagtagtaa aaaagagaaa
    45481 tattatcggt agtcccatct ttcagattcc atatattgga ctagtccttt caaaaacggc
    45541 atttgcatgg attcaggggt tattctttac gattgctttt tgcctaacag gaatcacagc
    45601 atggaatacc tttgtagagt tgaaaaagaa gcggagacga acacagaaat ttttgtaaga
    45661 atgttaaaaa cataaatgta ttccagagat ccaacgactc ttctgaacat gaaacacgag
    45721 aaaacactca tgcagcttgt agatttagtc agttgttcgt ataatgtgtg aagaggagga
    45781 gtagtatgcg caatgaaatg atgcatcgtc ctgtagtcaa agaagaacta ttggatttta
    45841 tgcgtaccca gcaaaaaaaa ttaccgggtg agctaggcaa actggaagaa gaagctcatg
    45901 tagcagaagt accagttatt ccccatgaaa cagttgtatt tttgaaattt ttacttggac
    45961 agcttcagcc tgaacgaatc ttggagatcg gagcagctat cggtttttca tcaagtgtca
    46021 tggccacgac aatcggagaa aacgctcatg ttacgacaat tgatcgtttc gatgtcatga
    46081 tcgagaaagc taaaaaaaac tatgaacgtc taggactgac agataaagtg actttgcttg
    46141 aaggtcaagc agcagatatc ttgccggagt tgtctggtcc gtatgacttt atttttatgg
    46201 atagtgcaaa atcaaaatat attgaatttt tacctgaatg cttacgtttg ttaagaaaag
    46261 gtggcgtttt gatggtagat gacatcttcc aaggaggaac gatcttgctc ccagatgaag
    46321 agattccccg tggcaaacga gctatccatc gtaaattaaa tgaatttctt cgtgttgtga
    46381 tggatcatcc cgatttgaca tcgacaatct tgccattagg tgatggtgtg atcttaatga
    46441 caaaggaatc agaaacaatc gagttgtgat acatgattat ggcagcctgt agataatcga
    46501 ttgaatcgag ggctcaattc gaacttgatg taaaagtttc tatatttgta agtcaattaa
    46561 aggatacagg aaatctaaat aaacaggagt agcatgaagt caatgtagct cctgttttca
    46621 attgataacc ttagaacagc tgaaggagaa tgtctcgttt taaaagattg atttagtagt
    46681 tgggccaaaa taattcatta tttgctaatt aaataactat ttcgtattga cagaagcaat
    46741 ttaattatct ataattattg atgttccgct gattcttgtc atagtagctc agctggatag
    46801 agcatccgcc ttctaagcgg acggtcgggg gttcgaatcc ctcctgtgac gctaaaaaac
    46861 cgttagaaac tgaggtttct aacggttttt ctttgttttt tgtgccaata ttgtttgttt
    46921 tcaaaggcaa ataaaataaa aaaacatcct tatcgagtac aattataagg agcaaaaaaa
    46981 taaaactcat cagaaaagag gaagaagata tgacgatact atctagaatt ttagtgactc
    47041 tagttgcaat tgaatttttt tatatcatgt atatcgaaac ggtgagaact gattctgaca
    47101 cgacaagtag ggtttttaaa atgagtaaag aagagttaag cagaaaatct gttcaaacat
    47161 tgtttaagaa ccaaggagtg tacaatggac tattaggagt gggactactt tatggagctt
    47221 atctttcttc cgcatctaaa gaaatcacaa gtatgctatt aatatctatt ttttttgttg
    47281 cattatatgg cagtttgact agcgatcgtt ggattttact caaacaaggt gggctagctc
    47341 tgttggcatt gctttctatt ttcttataaa tatagaaaaa ataagaattt atctaacagg
    47401 aaactttgtg aaaaatatta ctttatctca tgaaatatga tattctaatg aatggtattt
    47461 tagaaaattt catgaaaaag gagttagata ggtgttcaaa aattatgttt cctcactaaa
    47521 aaaagaattt tcaggttatg atctttcaag atttcagaaa gatcttttgg ctgggatcac
    47581 agtagctgct gtagcattgc ctttagcctt ggcttttggg gtatcaagcg gagctgatgc
    47641 ggccgcaggt ttgattacgg ctatcattgc cggtatcgtt attggcggtt tatcaggcgg
    47701 tttttatcaa atatcaggtc cgacaggtgc gatggcagct atcttgatgt ctattgcggc
    47761 aacaaaggga atgcaaggtg tattgcttgc tactttttta gcgggtctat ttttacttgc
    47821 agcaggactt ttccgattag gaacactgac ttcttttatc ccagcacccg taattacggg
    47881 gtttacttca ggtattgcta tcattattgc attaggtcag atcgataatt tatttggtgt
    47941 tcactcggaa ggtacaaacg ttattgaaaa actaagtagt tatcaaacga taggatttcc
    48001 gataaacaca gcctctttga tcatgggagg gctagtcatt ttagggatgt ttatctatcc
    48061 aaaaaaatgg gcgaaacgaa tgcctagttc gctgctggct atcattttgg caacagcagt
    48121 tatgctattg ttccatttac cagtagcaac tgtgggtaag attccacaaa cattgatcag
    48181 tagtaatcgt ttgaatatgg gagattttag cttttctgcc ttgcaacaag tggcagttcc
    48241 agctattagc attgctttat taggaatgat tgaaagcttg ctatgtggtg cttcggctgg
    48301 ccgaatggca aataagccct tagatagcaa tcaagaatta atggcgcaag gaattggaaa
    48361 tctgttgctt cctttctttg gcgggattcc tgctactgcg gccatcgccc gtacaagcgt
    48421 ggccatcaaa tctggtgcac aaactagatt agctggtatg atacatgcag ttgtcttact
    48481 gctctccatg cttgtttttg cgccagtgat gtcacagttt ccaatgccag cacttgcagg
    48541 tgttttgatt gtgacggctt ggcgtatgaa tgaatgggaa acgatcaagg agctatttac
    48601 taataaatat tggtcagcag ttctattgtt tatccttacg atgatttgta cagttatctt
    48661 tgatttaagt attgctatcg tgatcggggt gatcggcggc tgtgtatttt ttgttgtcaa
    48721 aagcgctgct atcacgattt cattagaaac gatcgattgg caaagaatgg ggctgccaga
    48781 aacggaaaag ctgaataact ggacagtagt gtatattagt ggtccactgt tttttatgtc
    48841 ttcagaaaaa ctcaaaaata aattggaaga actaaatcat cacgatggtg tcattttctc
    48901 catgcgaggc gtaccaagta ttgatttgac ggcacgtgca gtttttgatg aatttcaaga
    48961 aaaagcagag caaagaggac aaacgattat ctatacttcc ttgcagccag aagtagaaaa
    49021 acaactttct cacttgtggg aacatacatt cacgcatcaa catcaaacag ttgctcatgc
    49081 gttgacagct ttacatgcga aatatactgc aattagccat taaaacaatg aaaaagaaca
    49141 gacaagactg taaactgaag attttatggt cttgtctgtt ctttttaata ttgctgtaaa
    49201 agtgaagcta gtacttgcca gtcttgatgg ataacaggta caagggagcg attgtagaaa
    49261 atagctgctg gatgaaaaag tggaagaatt cgatagtttt tttctgacca ttcgtaggca
    49321 tccttttgtt ctgttaattg taagacaggc cctgtataga gttgaccatg atttttggta
    49381 accgtatatt ctttgccaag taaacgttga agaccaatgt ttccaagtgt cgcaattaat
    49441 tttggctgga ccttcatcag ttcgtaatcc aatattggtg catgcgcaaa tacttcggaa
    49501 cgagtaggtg tccgatttgg ataaacagtt tctgactgtt gtgtccgttt attgatccga
    49561 tgagtcacgc gatacgggcg gctccttaca gcactggtga tatacacctc ttctcgtgtt
    49621 aagccagttg aagaaagtgc ctgcatcaat tcttttccag cttgaccgct aaaaggaaca
    49681 tgcgtatcaa tctctgtttt ccctggggct tctcccacga gcatcagttt aggatgaaga
    49741 ggtccttgcc cagcgataaa tccttctaca ggacggccgt tcattcttaa agaagcttct
    49801 tgtatcagtt cttttgggta ttccatttat gctcctcctt tttcttttat tataagaata
    49861 aaagaaacac aatgcgacta ttttatttgg tagaactgtg aggatagaaa agcagaagta
    49921 ttgcttgaat ttttgtttta ctttttgatt ttaaatttag ttgactaacg gttgctgttc
    49981 tggtaagcta gtactgttgt aattgtggac tccacagcta caaccgctca gaacaagtaa
    50041 taagcacagc atatgctgtc acttcggatg gcgagtctaa gtatatcaga aggaggtgct
    50101 ttatagcatg tacgcaatta ttaaaactgg tggtaaacaa gtaaaagttg aagtgggaca
    50161 agcaatctat gttgaaaaac ttgacgttga agcaggagaa aaagttgttt ttgacgaagt
    50221 tatcttagta ggcggagaat ctacaaaagt aggcgctcca actgttgcag gtgcaactgt
    50281 tgaaggtact gttgaaaaac acggcaaaca aaagaaagtc gttactttca aatacaaacc
    50341 taaaaaacat tcacaccgta aacaaggtca tcgtcaacca tatacaaaag tggttatcga
    50401 cgcgatcaac gcttaatagc gtttgagaaa gaaggcctat atatgatcaa aggaacattt
    50461 aagcgaaatg acgcaggtca gatcgtttca ttcactttga ccgggcatgc tgatgctggt
    50521 ccttatggaa gtgatatcgt ttgtgcgggt gtttctgcgt tagcgatcag cactgtcaac
    50581 gggattgcct cattagccgg ttttgagccg attgttgaaa tgaatgaaga agaaggcggt
    50641 tatctttata ccgaagtcac ttcaggaatg acacaagaac aaaacaatat tgctgagatt
    50701 ctattagaaa atcttttatt aggattacaa tcgatagaag ctgaaaactc agaatatatc
    50761 caaattaaaa caattaatga taaataggag gtgcagacca tgttgttatc tatgaatcta
    50821 caattatttg cccacaaaaa aggtggcggt tctacatcta acggccgtga ctcagaatca
    50881 aaacgtctag gtgctaaacg tgctgatgga caaacagtta caggtggatc aattctttac
    50941 cgtcaacgcg gtacaaaaat ctatccaggt gtaaacgtgg gtatcggtgg agacgacact
    51001 ttatttgcaa aagttgatgg cgtagtacgt ttcgaacgta aaggccgtaa caaaaaacaa
    51061 gtgtctgttt atccagtagc tcaagaagct taattgaatc ttatgattaa agaggttgtg
    51121 acaagacttt tgtcacagcc ttttttgttt ataaaaataa aagttgtagt ggaggctacg
    51181 aagtttactt tgtcttttta cacttaatat ttttttgcca atttggaatc aggatgtttt
    51241 gataactatc gttttcaatt gatggaagct atattgtttg ctatcagtga aataattata
    51301 tggtaaacag tgttcttttt cttgtttcca ttataaaaat cgtatcggga tttttcgttc
    51361 ttttaaattg aacatttcta tctatccgcg tgttatcaac aaaacaaaat caggtaaata
    51421 gttgacgaaa cagaaataaa tacatagtat tatgttttga agcctaagta caatagtcta
    51481 tagaaaagga gcgataccat tggcaaaacg cattgaccga atttatatgt atgtcaaaga
    51541 aaaaaccgct catttaacaa aggcagaata tgatcaagga gtaacaacac aggaaattgc
    51601 tgaagtgtta gggattcagc gaacaaatag cagtaaagat ctgaatcagc tagtgcgcga
    51661 aggaaaactg ataaagacag acggacgacc agtacgttac atatatcaaa cactcgttac
    51721 gcacgtaaag ccagttacaa aacatgtagt cagttacaaa gaaacgccgg tctctgaaga
    51781 aaaaaataca ctccaaatag atactaagga tatctttgca aaaatcattg gagctaacgg
    51841 cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc caagaggatt
    51901 aaattgtttg attacaggac ctaccggatc aggaaagact tattttgcac acgcgatgta
    51961 tcattttgca aaagcgcatc atgtaataga tgaggaaacg gaactgattg ttttcaattg
    52021 tgctgattat gccaataacc ctgagctttt gatgagtcat ttatttgggt acgcaaaagg
    52081 tgcttttaca ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg atggcggtat
    52141 gctgtttttg gatgagattc accggttgcc gcctgaaggt caagaaatga tcttttactt
    52201 tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg aagcaaatgt
    52261 acggatcgta ggtgcgacca cagaagaccc aggttcttct ctattagaaa cttttgttcg
    52321 gcgtatccca atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa atgagaaagt
    52381 cgatttagta aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa agatttcttt
    52441 gacagaagat gtagcaaaag cattgatcgg cagtgtgacc tatggaaata taggacaatt
    52501 gaagtcaaat atccagttag tatgtgcacg aggatttcta aatcatatgc aatcacccga
    52561 aatctcgatc acaatcgatg atttgacaga agggattcgt agcggattga ttcaattagc
    52621 aagcaaccgc agtgcaatgg cagagctttc taaaatattg gagccaaaga taactgtagc
    52681 accaaatgat acgatcatga aaatccaatc ggactcctat gagttacctt ataatctata
    52741 tgatatcatc ggagataaag cagctttact gaagtcggat ggtttggatc aagaagcaat
    52801 caatcatttc atttctacag atatcaacat ccatctgaag tctttctata aagaccatgg
    52861 tttttctttt aatgcagaca gcaaattagc agaatttgta gatccgaaaa tcatagaagt
    52921 gaccaatcag atttatgtaa tggtcaaaag atctttacca tatgagtttc agcaaaactt
    52981 tatttatgct atgagcttgc atatcagttc ttttttgaaa aaaatcaact taggagaaga
    53041 acggcatacg aatgataaca tccgtgagat ggcttttgat tttccagatg aatatgctgt
    53101 tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa gtggagattc cagaaagtga
    53161 agactattac ttgaccgttc tactcgtctc gttacgggca aatcaagcaa gcggcagaat
    53221 cggtgtagtc gttgcagctc acggaaacag cacagcaagc agtatggtcc aagttgtcca
    53281 acagttgtta gatgcagaga acgtacgagc agtagacatg cctttggata tggatccgaa
    53341 aacagcattg gaccgaattg aacgcaatgt acaagaagta gatgaaggaa gcggggtcat
    53401 actcttagta gatatggggt cccttgcgtc gtttaattcg caaattcaaa gagacactgg
    53461 aattccagta cgtactgttg atatggtaac aacttctcta gttttggaaa ctgtacgaaa
    53521 ggtctcagta ttagggacag atctagatat gttgcatgaa tcattgaaaa attttcgagg
    53581 ttacgcagaa atatcttctg tagaatctgt tcaggaagca aaacctattc ataagaaaaa
    53641 agctgttttg gctatctgcg catctggcga aggtacagca caaagaatca aagaattgat
    53701 cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa gttctagctt tgtcgattgt
    53761 ggaagtgaaa gaagaactgc cagttatcca aaaagaatat cagatcatag ctactactgg
    53821 gattactgat ttgaatatcc aagccccatt tattccttta gaacgtttta ttgatcaaaa
    53881 tatcgaagtc attttggatc agttgctgat ggaatcagaa ttggaggaaa ctgaattcat
    53941 ctccctagat gaagagtcag ctaaaaatac gtgtgttgag tttatctctg ataacttcat
    54001 ctttattaat ggtagtaagt taatcgatcc aatgtggcaa ttttctacac aaatctctca
    54061 gacgacggga atcggcgatg aagaatatgg attcaaaatc aacctagtga tgcacacagc
    54121 aggaatgatc gaacgtatta ttcggaatga accattgact gtggaagaaa acgaattgac
    54181 aaatacaacg aatgatcctc tttatagcca attggcagcc tcagttgttc tgttggaaga
    54241 tcagataaaa gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc tagtccataa
    54301 tcagttagat aaaaaagaat acactgtgcc atgaatacac aaaaacgata cacttttata
    54361 gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt caattagtgt atcgtaaaaa
    54421 agttggcacg ctttttgctt atagtagtgt gtgctaggag gtggttgttc acacagaaat
    54481 gataggggtt tctgtgaagt agtgaggaag aaggagtttt gcagatggat attcgattgg
    54541 cacgtataga tgatcgttta atccacgggc aagtcgcaac agtttggtcg aagcaaacag
    54601 gtgttgaacg tatcattgtg atcagcgatg tagtcgctca agataaattg agaaagtttc
    54661 tactaaaaga agctgcaccg ccggggatca cagcaaatgt aataaccgtg cagaaaatga
    54721 ttcaaatcta tcatagtgga ttattatcac gagcaaaagt gatgctttta ttcaccaatc
    54781 cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg aatatcggcg
    54841 gtatgagttt tactgaaggc aaatcaatga ttaccaattt tgtttcagta aatcaacaag
    54901 atattgattc ctttcgttac ttgaacagtc aagaagtgga gttagagatt cgaaaagtac
    54961 ctgctgacag gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag gtgtagcaag
    55021 aatggttgtt ggaaaaggtc aaataattct gttgtaatgg ttagcaatta ttaatatttt
    55081 tgaatttgtt acaacaaaag taaataggag gtataaatat ggtagggatt atcctagcaa
    55141 gtcatggaca atttgctgaa ggaatcttac aatccggttc aatgatcttt ggcgaacaag
    55201 aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg agagccaaat
    55261 tggaagaagc ggttgcttct tttgataatc aagacgaagt gttattctta gttgatttat
    55321 ggggcggaac accgttcaat caatcaaaca cattattcga agagcacaaa gataaatggg
    55381 caatcgtcag cggtttgaat ttaccaatgt tgattgaagc ctacgcatca cgcttttcaa
    55441 tggaatctgc tcatgaaatt gcagcacaca tcattgaaac agcaaaagac ggtgtaaaag
    55501 taaaaccgga agaattagaa ccggcagaag caccgaaagc agcagttgaa gacgcacaac
    55561 ctaaaggagc acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa tatgtattag
    55621 cacgtgttga ctcacgtctg ttacatggac aagttgcgac agcttggaca aaagcagtgc
    55681 aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa ggatgatctg cgtaagagac
    55741 taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt tattcctatt agcaaaatga
    55801 tcgaagtagc aaaagaccct cgttttggaa ataccaaagc attgctttta tttgaaaatc
    55861 ctgaagacgt attgacagct gttgaaggtg gcgtagacat caaagaatta aatgttgggt
    55921 caatggcgca ttcggtcggt aaagtcgttg taagtaaagt attgtctatg ggacaagaag
    55981 acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta cgtaaagtac
    56041 ctaatgattc acgcgataac atggatgata ttttgaaaaa agcaaaagct gaattagcaa
    56101 aagcgtaata aataatatta aaatttatag gaggcttatc atgtctatta tttcaataat
    56161 tttagtcgta ttagttgcct ttctagctgg tatggaaggt attcttgatg aatttcaatt
    56221 tcatcaacca ctagtggcat gtacattgat cggtttagtt accggtaacc tcgaagctgg
    56281 tatcgtactt ggcggtacgc ttcagatgat cgctttaggt tgggcaaata tcggtgccgc
    56341 tgtagcacca gatgctgcat tagcatcagt agcatcagca attattttag ttttaggtgg
    56401 acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg ctgttgcagg
    56461 gttgttctta acaatgatcg tacgtacaat cgctgttcca atcgttcatt tgatggatgc
    56521 ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg ctgtatgttt
    56581 acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta tttatcccag cacaaactgt
    56641 gcaatcattc cttgaatcaa tgccagcatg gttaacagat ggtatggcta tcggtggggg
    56701 aatggtcgta gctgtaggtt acgctttggt aatcaacatg atggctacaa aagaagtatg
    56761 gccattcttc gttattggtt tcgttgtagc tgcaatttct caattaacat tgattgctct
    56821 aggtgcttta ggtgttgcat tagctcttat ttacttgaac ctttctaaaa tgggtggttc
    56881 atcaaataat ggaggcggaa gcaactctgg tgacccttta ggcgacattt tgaatgacta
    56941 ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc aaaaaaagat
    57001 cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt catggaacta cgaacgtatg
    57061 caaaacggtg gctgggcatt ttctatgatt ccagcaatca aaaaattata caaaacaaaa
    57121 gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct ttaatacaca cccatacatt
    57181 gcttctccaa ttttaggggt tacattggct ctggaagaag aacgtgcgaa tggcgcgcct
    57241 gttgatgatg tagcgattca aggggttaaa gtaggtatga tgggtccttt agctggtgtt
    57301 ggtgacccag tgttctggtt tacagtacgt ccaatgctag gtgcactagg tgcttcatta
    57361 gctatgggag gaaatatcct tggaccaatc atcttcttct taggttggaa cattattcgt
    57421 tgggcattca tgtggtatac acaagaattt ggctacaaag ctggttctaa aattactgat
    57481 gacctttctg gcggattgtt acaagacgta acaaaaggtg cttcaatttt agggatgttc
    57541 gtattagctg cccttgtaca aagatgggta tcaatcaaat tcttaccagt tgtatcaact
    57601 gtaaaattag ataaaggtgc ttacatcgaa tgggataaat tgccttcagg tggtgaagga
    57661 atcaagagtg catttgaaca agttaataat ggcttagccc tttctcctga aaaggtaaca
    57721 acattacaag acaacttaga ccaattgatt cctggtttag ctgcattagg attaacattg
    57781 ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct tggtttattc
    57841 gtagtcggtg ttgttggaca cgtcatcgga cttttataat ccataaaaat atagctcggg
    57901 cgttgtcccg agcttttttt atatagtata ctgttcataa acaaatatct ggagggagaa
    57961 aatggttcaa tcaatcaata caaaagtaga cttggtaatt gatgctactt cgtttacggg
    58021 tctgacagat tacgggcaaa tcatgatagg taataatggt tttgagtttt accatgcgcg
    58081 agatagacgc aaatatatcc aaattccatg ggaagaagtg gattatgtac tagcatcagt
    58141 catgctaaag ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa atggtacata
    58201 cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat atgtagatga
    58261 agatagaatg gtacaatcat tgagtttctt tgatgtagtg aaacgtggta ttcgtagtat
    58321 gtttaaacgc aagtaaaaaa taaagaaaac ttctaagagg aggttttctt tattttttta
    58381 ctttgcagaa gaaaatatga aatagcaaaa ccaattattt gtccccttta aatcccatat
    58441 gctaaaattg attagcaaag aaggaggtta tattatgagt tggttatgga cattgattgt
    58501 tggagccgtg atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt cgatgggctg
    58561 tgtaacgaat atcattgctg gactagtcgg ttcaagtatt ggacaagcga ttttcggcga
    58621 atggggacca aaagctgcag gtatggcact tattccatct attttaggcg cagtgatttt
    58681 ggtagcagtt gtctcatttt tctttggaaa acgatcataa gtttttacaa aatagtaaac
    58741 taaaaggagt tgcagcgaga ctgtgactcc tttttggctt tatttattta aaataaaaag
    58801 aagttttccg gaaattttta tttaactgtc gtgtacgaag aaaagtttgc tataatggaa
    58861 aaaagcgtgc aaagaaagag ggagtttcat tatgatgcta cgtgtagaaa aattgcgcaa
    58921 aaaaatgcaa gaagaaaatt tggattcatt tttggttacc agtccgtaca atcttcgtta
    58981 cttaacgaac tttacaggta cgacaggatt agctgttatt actttggaaa aagcattttt
    59041 tattactgat tttcgttata ccgagcaggc ggcagcgcag gctcaagggt ttgaaattat
    59101 taaaaatgtc ggacctattt ttgatgaggt agcaaactta gtacgcaaag aagaactgca
    59161 ctcattagct tttgaggaaa caacagtctc atttttagaa tattcggttt tagaagaaat
    59221 catcgatgcg gaattagtac ctgtttcaca gatgatcgaa gaactcagag aagtgaaaga
    59281 tgaagaagaa atcgctatta tcgaaaaagc atgtcatatt gcggatatgg catatgatca
    59341 catcctgaaa atgatccaac cgggcatgac tgagattgaa gtcgcaaatc aactagactt
    59401 ttatatgcgt tctcttggcg cttcaggtgt ttcttttgat acgatcgttg ctagcggatt
    59461 gcgttcggct atgccgcatg gtgtagcaag caaaaaaatc atcgaacaag gtgacttgat
    59521 cacattggac tttggttgct attacgaagg gtacgtttcc gatatgacac gtacattcgc
    59581 tattggtgat cctggggaaa aattaaaaga aatttatcaa atagtcttag atgctcaatt
    59641 agcagttatt gatgctgcaa aaccaggaat gactggtgtc caattagatg cagttgctag
    59701 agattatatt tcaaaacatg gttacgggga agcatttgga cattctacag gacatgggat
    59761 cggtttagag atccacgaag gaccaaacgt ttcttctcgt gcagaaaagc aatttgtacc
    59821 aggaaatgtg atcactgatg aaccagggat ctacttacca ggaatcggtg gagtacgtat
    59881 tgaggatgac ttgttgatca cagaagatgg aaatcgtgtg ttgactcgtt caccaaaaga
    59941 attgatcatc ttatagtttc atcaatagaa gaagtaagcg taaaaatcag tgttcacaga
    60001 ctttgttata gaaaatcact cgatttaatg atacactata gaagaacttt taagctgtaa
    60061 gagataataa agttgggtgt tttctccaat tcatagcagt ttgcagctga tcggcggcag
    60121 ttttcttacc acgtgatcac tatgccgcat catagagaat tcggatgtat ctcgtgctta
    60181 ggcaaaggga ggaataaata aatggcagat gaaaaaaatt tgattttaaa cgcaaaccaa
    60241 gatttaggag agatcgtgat cgctcctgaa gtcatcgaag taattatcgg gattgcagct
    60301 tctaaagtag aaggcgtata cggaatgcgc ggaacttttg ccagcaatgt cactgaatta
    60361 ttaggacgtg cagctcacgg aaaaggtgtt tacttagcaa acgaggaaga tggcttgaaa
    60421 gtagatcttt actgttatct agaatatggt gtttcagttc ctaaagtagc aatggaaatg
    60481 caggaacggg tcagccagca agtccttttc atgacggata tcgatttatc tgaagtcaat
    60541 atccatgtag tagctgttgt tcctgaaaaa ttagaacaac caacaatcga agaacttttt
    60601 gaagacgaag aggaagagaa tgaataaaga attatcaaga cacgagattc gggaaatggc
    60661 attgcaggct ttatttccat tagacttcaa tgcagaccta acaaaagaag atgcgatttt
    60721 caacgcaatc gaattagacc accgcgacat gatcaacgaa gatgaatcag agtttgttcc
    60781 tgtttatctt gatacgctag ttggcggtgt ctgtgcaaaa aaagatgaat tggataaagt
    60841 gattgaaaaa catttaaaaa acaattggca catcaatcgg atctcaaaaa tggatttggt
    60901 gatcttgcgt atcgcgattt ttgaaatgac ttatgtgaca gatgtaccag cggctgttgc
    60961 tttaaatgaa gcaatcgagt tggcaaaaac atttagtgat gaccgttcac gtaaattcgt
    61021 caacggtgtt ttatccaatg tattgaaaga attagaagct ggggcgtaag tcctagcttt
    61081 tttctatgaa tcatcataaa cctgttataa atcgggaaaa ttctatggta gaatgaaaac
    61141 acaaaaaata tctgggagtg gttagatggc agaattgatt aacggaaaag aattagctga
    61201 aaaaatgcag gcagagattg ctgaaaaaat ccaaaaattg aaagataatg gtatccatcc
    61261 cggattagtc gttttattgg taggggaaaa tccagccagc caaatctatg taagaaataa
    61321 agaacgtgta gcaaaagaaa tcggaatcca ttcattggta gagcgttatc ctgagacgat
    61381 ttctgaagac gaactacttg cagaaattga aaaatacaat caagatccta gttttcatgg
    61441 tatattggtc caattaccat tgccaaagca tatagatgaa gaaaagatcc tgttagctat
    61501 ccgccctgaa aaagacgtag acggttttca tccgatgaat ttaggcagac tccttgcggg
    61561 taaaccggac aagattcctt gtactccata cggcatcatg aaaatgtttg aagcttattc
    61621 tatcgatccg gcgggtaaac gagcagtagt tatcggacga agcaatatcg tcggtaaacc
    61681 gatggctcag ctactcttga tggcagatgc aactgttact gttgctcatt ctaaaaccga
    61741 aaatctggct gaactggcac aagaagcaga tattttagtc gtagcaatag gacgtggaca
    61801 ttttgtcaca aaagaattcg taaaaccagg tgctgtagtg atagatgtag ggatgaatcg
    61861 tgatgctaac ggtaagctga tcggtgatgt gaaatttgat gaagtggagc ctattgcagg
    61921 ctggatcact cctgtgccaa agggtgtagg gccaatgact atcacgatgc tgatgtatca
    61981 aaccgtaaaa tctgccgaag cttttggagc aggtgaatag atggcacaag aatatttaac
    62041 tgtcactaca ttaacgaagt atttgaaaag aaaatttgat gccgatcctt atttagaacg
    62101 cgtatattta acaggcgaaa tatcgaattt ccgtttacgt gccaatgccc atcaatattt
    62161 cagcttaaaa gatgaccatg caaaaatatc tgctattatg ttcaaaggag cattccaaaa
    62221 attacgattc cagccaaaag aaggaatgaa agtccttgtc gttgggcgta tttcattata
    62281 tgaagcggga ggttcttatc aaatctatgt agagcacatg gagcctgatg gaataggtgc
    62341 tttatatcaa gaattggaag aacgaaaaga aaagctgatg aaggaaggcc tttttcaagg
    62401 gcctaaaaaa atgctgccac gtttcccaaa gcgaatcgcg gtccttacca gtccaagcgg
    62461 agcggttatc cgcgatatca tcacgacggt gaaaagaaga tatccaattg tccaattggt
    62521 tttgttccca acagttgtac aaggaaacca agcagcggac aatgttgtca ggaatatcca
    62581 gagagtagaa gagatgggca atttcgatac aatgatcatc ggtcgtgggg gtggttcgat
    62641 tgaggattta tggccgttta atgaagagcg ggttgctcgg gcgatttatc aagctaatac
    62701 gccggtgatt tcatctgtag gacatgaaac agataccacg atcgctgatt tagtagcaga
    62761 tgttcgggct gccactccaa cagcagctgc agaattagct gttcctgttt tgtcagaaga
    62821 gttgatgcgc attcaagaac ggcaagctag attgcagcag gcgtttgtac gccagatcca
    62881 gcgcaaacaa gaacggttcg agcgtgctcg acgttcctat gtatttcggc agcctgaaag
    62941 attgtatgaa ggtcagacca tcaaattaga ccagttgaaa cagcgattat atcaagctac
    63001 acagcaaatt tatcatgcga aagaaaaaca aacgatcgca ttaagccacc aattggaaca
    63061 agtggctccg ctttatcgag ttaaagcagc tcgccaagaa actgcttatc tagaaaaacg
    63121 cttagaagag aaaatccaac aatatatgaa acaacagcag cagcgttttg ctcaagctgt
    63181 tcaatctctt gatttgttga gtccattgaa aatcatggga agaggctaca gctatacgac
    63241 gttaaatgat caagtagtaa aaagtgtcac cgagattcat caaggggatt tgctgacgat
    63301 ccattatcaa gatggaatcg ttaaaggaca agtacaagag atagacaaag gagaataaaa
    63361 atatggaaaa tccaactttt gaagagtcac tgcaagaatt agaaaaaatt gtcatgcagt
    63421 tagaacaagg agacgttcca ttagaatcag ctctagattc gtttaaacga ggaatggaat
    63481 taagcaaaca ttgccaagat acattagcta aagcagaaga gaccttgacg aaaatgatga
    63541 cagaatcaaa tgaagaagtt gattttgacg gaaacgagga aagctgagat gagattaact
    63601 gacttttcag ctattcatct acctgctgta gaaaaagaaa tcttgtcttt tttagatgag
    63661 catacaacgg atagaggact ctatgatgcg atgagttatt cagtgaaagc aggcgggaaa
    63721 agaatacgtc ccttattact tttaacggcc gttgcttcct ttgatgagcc tattgatgtt
    63781 cccgtctatc aagtagcagc tgctttagaa atggtgcata cgtattcctt gatccacgat
    63841 gatttgcctg cgatggacaa tgatgatctt cgtagaggaa aacctaccaa tcataaagta
    63901 ttcggcgaag cattagctat attggccgga gatggtttgc ttacaggagc attccagcta
    63961 atcagtatgg cacacttagg taattctcct aagcttctgc tgcttcagca attagctgtt
    64021 tgtgcgggat cacaaggtat ggttgctggg caagctgcag atatcgaggg cgaatcgaaa
    64081 aagctttctt tagaagaatt agcatttatt catgaaagaa agacagggca tttgattcgt
    64141 tatgccttgc tggctggtgg catattagcg aagcaacctg aagaaatctt attgctactt
    64201 caacgattag ccgaacattt aggtttagct tttcagataa gagatgatct actcgatgtg
    64261 attggtacaa cgaaaacatt agggaaaaca gctggtaaag atgaacgtat ggaaaaaaat
    64321 acttacccgc gtttattagg cctagaaaag acacgtgagg cgttggagat tgagctgtta
    64381 tccgccaaca agatcataga caaattagaa gaggacgttt tttcttttga tggcgggctg
    64441 atgcgccaga tgatcaagca atttgatgtg gagaaatgag ggaaagaatg gaaaaagaac
    64501 gcgtagatgt attagcggtc aaacaaggat tatttgaaac aagagaacaa gccaaacgtt
    64561 cagtgatggc tggtttgatt tacaacgaaa aaaacgagcg gtttgataaa ccgggagaaa
    64621 aaattccagt gagtagtgaa ttaagagtca aaggtaaaaa acttccttat gtttctcgag
    64681 ggggattaaa attagaaaaa gcactcaaag catttgatct atctgttgaa ggaaaaacat
    64741 tgttagatat tggtgcatcc actggcggat ttactgatgc agctttgcaa aatggcgcaa
    64801 aaatgagtta tgctttagac gttggctaca atcagctagc ttggaaaata agacaagatc
    64861 ctagagtcgt cgtaatggaa agagtgaatt ttcgttatgc aaaacccgaa gactttacct
    64921 taggaattcc agaagttgct gttatcgatg tttcttttat ttcactaaaa ctgatgctgc
    64981 ctccattaca tgctatttta aaagaagagg gagaagtgat tgctttaatc aaaccccaat
    65041 tcgaagcagg cagagaagct gtagggaaaa acggaattgt tcgtgaccct cagacccatc
    65101 aaaaagtttt agaagacatt ttgtcatttg cggcacaagg tggatatgat gtgctggaat
    65161 taagttattc tccgatcacg gggggagaag gaaatatcga gttcttggct cacttgagga
    65221 aagtacccga aagcggaaca atcaattcag ctattaatat ggcagaagtg gtttcaaatg
    65281 cacacgaaca gtttgaccat aaataaattg taactgtgaa aatcaagtga gaagtatgcg
    65341 gattcttctt tattatacca aggaaataag ctatcattga ttaagaagaa tatgctaagg
    65401 agcgaagacg taatgagaaa aaaagatcgg catcgtttga ttactcgatt gttgagtgag
    65461 caagatatcc gaaagcagga agaatttgtt gaaatcctga aaagcaaagg aatatctgtt
    65521 actcaagcta cgatttccag agatataaag gaactgaaac tgatcaaggt accggcttca
    65581 gatggcggct accgttatag cataccagct gaaacaaacg aagatgtaaa tactaaattg
    65641 gaaaaactat taaaagacgc tttcgtggca gttgaccaaa tggaaaaatt tgttattttg
    65701 aaaacattgc cgggaaatgc ttcggcagca gcaaatttga ttgataaacg ttacaagaaa
    65761 gaattatttt cgattatcaa tgatgacgat aatgttttga tgattacacg aactgaagac
    65821 caggcagttt tattgaagaa agacttcctg cattaccttt aagttgagga gcgaatgtga
    65881 tgctacaaga aatcagtatt actaactttg cgattattcc ggaattgcgg ctttcttttc
    65941 acgaaggaat gaccgcgctg accggagaga ctggtgcggg taaatccatt attatcgatg
    66001 ctttaggtct gttagcagga ggaagaggtt caagcgatta tatacgtcaa ggagcagaaa
    66061 aatgtgtgct tgaagggttg tttgaactac caaaacaaga aggattctct gaactgatgg
    66121 ttgaattagg gatcgaaaca gatgaggaca atctgattgt tcgacgggat atgtccttaa
    66181 ctggaaaaaa tgtttgtcga gtgaatggtc atattatcac attagctaat ctgagaagaa
    66241 tcggcagtta tttagtagat atccaaggac aaaacgaaca tcaggaactg ctccagcctg
    66301 aatcacatct tgctttattg gatcgttttg gagatgtagc tttccaacag aagaaaaaga
    66361 gttatcagca agaatatatc tcttatcgtg agctggaaaa aagggtacga aaagtgcagc
    66421 aaaatgaaaa atcctatgtt cagagaatag atatgctcca tttccagcaa gaagaaatag
    66481 ctgctgctga tttgcaagtc ggagaagaag agaaattaaa agaagaaaga gaaaaactta
    66541 gcaattatca aaaaattgct gatggattag ctgcaggtta cggagcactg acgaataatg
    66601 aacaaaacag tgtagatggt gttggcttgg ccgtttcaga actgcaaggg atcgcccatc
    66661 tagatgtgga gtatgaagca atttatgaaa atatccaaag tgcttactat ttgctgcaag
    66721 atgcaatcgg cgatatgagt cgtcagattg atttattgga gttagatgag agtcgtttag
    66781 aagaagttac gcaacgttta gaattaatcc gacagttgaa acgtaaatac ggcgaatcca
    66841 ttgagtcaat attggcttac tatgacgaaa tcaccgaaga gctagcttct tctgattttt
    66901 cagaaggtca attggacaaa atgaaagagg aactagagca aaaagagctt ctacttcagc
    66961 aacaagccgc agatcttcat gaagcgcgta aaaaaatcgc aaaagaattg gaaaaatcca
    67021 ttctgcatga attgaaaagt ctgtatatgg aaaacactga gtttgaggta cgtttcttaa
    67081 aagaagaaaa tagacaattg aatcatgatg gatttgacca aatcgaattt tacattacga
    67141 ccaatccagg agagccactg aagcctttgg tgaaagtcgc ttctggtggc gaactgtctc
    67201 ggatgttgct tgctttgaaa actatttttt cttctgagca aggtgtgaca agtattattt
    67261 ttgatgaagt ggatacaggt gtgagcggaa gagttgcaca ggccatcgca gataaaattt
    67321 ctcaaatatc gaaatactct caagttttgt gcatcactca tctgcctcaa gttgctgcag
    67381 tcgccgacta tcaatattat attgtgaaag cagtgattga tggacgtacg caaacttctg
    67441 tttcagagtt gaaaacaaaa gaaagagaag aagaaattgc acgtatgctg gctggaagtg
    67501 agattacaaa actcacactc gaacatgcaa aagaactatt gaaactcgca aaaaggacct
    67561 gagccacctc gcagtggttc aggtcctttt cagcagctga tagctatatt ttatttttac
    67621 atgacactca ttacagcagt caatacaaca gatccaatcg ttaaaatcag cattgtccaa
    67681 atgacaaatt ttgttacttt agcaaaagta cttgtttgtt ttttttcacg cattgttttc
    67741 aacctcgtca ttaattagtt gattcaagtg tacaatcttt tttccatttc cacaagaggg
    67801 cttttttata aaaacttccc tttttttaaa taataggtag ttacgataca aataaataat
    67861 gttaatagaa ctagccatat aaaggcatca tccctttcag caataggtaa cttaatgttc
    67921 attccataga tactgccgac gattgtcggt atagttaaaa taatcgttaa agaagttaaa
    67981 attttcataa tattattcag attattcgat acgattgctg aaaaagtctg attcatttgt
    68041 tctactagtt ttaattgaat gcgagaagta gtcattgcct gctctgtttc caccaataca
    68101 tcacgtaaat gatttttcaa agcatgattt tcttggaaaa gtttattttg cgctagttct
    68161 ttcaatgcat ctaaattagc agaagtcgct tctctgaaat aaacgaggct tttttggata
    68221 tccatgattt gataaagttg tttgttttca gtggatactt gaagtttggc ttccaaagta
    68281 tcgcattgag attgaagcgt agctaaataa ctgttgtaag agagtgtgac ctgccaaatg
    68341 aattgaaaga agatattgag attaggcgaa atatcacttt ttggaaaggt ctggctaaag
    68401 actttcttta aaaacagtgg ttcatgattc gtaattgtga taagcttctt tttgggcgtc
    68461 aaaataatag agagcgggta tgtatccact tgaaaataac cgctaggact tgttttagca
    68521 aaaggatatt gtaacaataa taacgctggc tttgtcaact ttgattgatt gaattcttct
    68581 gtacgggaat tttctgcatc gtccaatatg gaagtgatgt aatctgacgg aatttcaaat
    68641 tgatgtgtca gctgatgaat ctcttcttga ttaggacgtt ctacacaata ccagatcgtt
    68701 tggtcagttt cctttccggt attacgcagt ttctcattat ctaatgtaaa atatttgatc
    68761 atgattttcc tcctgttgcc tgtttctttt attataacgg atagggaatt ggaatacatg
    68821 gaatgtgttt gtaagatata gaatataact gcataaattt aaataaaatg aaacagttgt
    68881 gtaaagtata tacagtatac ttgaagcaac gataaaaaag gagtttacgt tcatgggcac
    68941 attcacacca cttatcttgt tttggtatat ttacccggta atcgttttgt ttggttgcca
    69001 atttcttgta aaattatttt cgttaaatcg tcgttttaaa ataaaagccc ctgatttggc
    69061 gattcctttt ttgtggggcg gtatgcatgc attgtctaga aatacaggga caatttctat
    69121 cttaccgttt ttctttattt ctgttttgct aatgggaatt tgtattgctg ttttccaagc
    69181 atattattat gaagaaatta tttatccgcg ttatttcaag atgacttggc gtttggtttt
    69241 tctgttgaca atcgtcttgt acgctgtctt gatcattgtg aacatacttt cttacctatg
    69301 aaaaaaatag aaaagctgta aaaagtcttt taaaaggctt tttgcagctt gatttttaaa
    69361 aaaatgttta tgaataaaaa aagttgttcc cggatttccc agaaaaggtg gtagaaagtg
    69421 ggggattgtg gtagactaat tttagttagt ggaggaatgg ggggctttca atgttgatgg
    69481 gtgaattcca acataatatt gatgcaaaag gacgtttaat cgtgccctca aaacttcgtg
    69541 aagaacttgg cgaaaagttt gttctgacac gcggattgga tggctgtctt ttcggctatc
    69601 cgatgagcga atgggaaaat ctggaagcta aactgaacga aatgccgttg gctaaaaaag
    69661 acgcacgtac ttttgtgcga ttcttctatt cggctgccac tgaatgcgaa cttgataaac
    69721 aaggacgaat caatataccc agcacgttaa gaaattatgc agctttgaca aaaggttgcg
    69781 tagtcatcgg tgtatctaac cgaattgaaa tttgggatga gacacgatgg caagattttt
    69841 ctgcagcagc agaagagaac tttgacgaaa ttgctgaaag tatgattgat ttcggactat
    69901 aagaatagga agaggctgac taatgactga aacatttcaa cactatacag tcatgttgaa
    69961 agaaacggta gatggattga acatcaaacc ggatggcgtt tatgtagact gtacactcgg
    70021 cggtgccgga catagtgaat atttattaag ccaactaagt tctaaagggc atctgtatgc
    70081 gtttgaccaa gatcaaaaag caatcgatca cgccaagctg cgtttagctt cttatgtcga
    70141 acaaggtatg gtcacattta tcaaagcgaa cttcagagaa ttagaagaac gcttgagtga
    70201 gcatgtagat aaggtagacg gcattttgta tgatctaggg gtttcctctc ctcagctaga
    70261 tgaagcagaa cgtggattta gttatcatca agatgcaccg ttggatatgc gcatggatca
    70321 gtcagctcct ttatccgctt atcacgtaat taatgaatat tcctaccatg aattagtaaa
    70381 gattttcttc cgctatggcg aggagaaatt ttccaaacaa attgctcgtg aaatcgaacg
    70441 agtacgaaaa acaaaaccaa tccaaacaac tggtgagctt gtcgaaatca ttaaacaagt
    70501 catcccagct cccgcaagaa gaaaaggcgg gcatcctgca aaaagaattt ttcaagctgt
    70561 gcggattgca gtaaatgacg aactcggtgc cgaagaggcc tctttagaac aggcaatccg
    70621 gttattgaaa gtcaatggaa gaatcagcgt aatcacattc cattcattgg aagacagaat
    70681 tgtcaaaagt atgtttaaag aatatagtac ggtacaagat ctaccaccgg gacttcctgt
    70741 catacctgac gaattccaac cagaactgaa agtcattact cgtaaaccaa tccttccttc
    70801 agaggaagag ctaacagaaa acaatcgatc acgcagtgca aagttacgta ttgcagaaaa
    70861 aataagaatg aaagaggagt agaatgatgg cagaattaaa gaaaatgcaa gattacgaat
    70921 atgatttacc tgaattggtg gatcagccgg aacaacaaat gcctgattct tcttcgaatc
    70981 aagaaatcat tcttccacaa tcaccggcaa aacgattaaa gcagatttct gctttggaaa
    71041 aaattgctgt ggcaagcgtg gcgattgcta ttgttgcttt atgtgtttta acagttatgc
    71101 tgcgcacaaa tatcagcgga gctgaaaaag aaatcacgtc aatacaaaca gaaatcaatg
    71161 ataaaaagca agaaaaaacg agtttagaac aagaaaaaaa cgaactttct cgtactgaac
    71221 ggattaaaca aatcgcagaa gaaaaaggat tgtctattaa tgacgacaat ttaaggaaag
    71281 tgaagtaaat gagtcttaaa aataaaattc ggcggtttat ggaaaagaaa aatctaaatc
    71341 cgatgaacaa tcgcaaaaaa gtaggtatca ttttatttgc tacgagtatt ggattgttct
    71401 ttttatttgc cgtccgtttc tcgtatatcg ttattggagg tcatgttgct ggtacctcat
    71461 tagctgaaaa aacaaaacag ttgtaccaag gaagtgaagt agtaaaagca aaaagaggaa
    71521 cgatttatga tcgtaacggt gtggcgctcg cagaagatgc ctcctcttat tcaattaaag
    71581 cgatactctc aaaaacatat acctctgggg ataaaaaact gtatgttgaa gaaaagaatt
    71641 ttgataaaat agcagaaatc ctgcataaga atctttctat agacaaaaaa gatgcactga
    71701 atattttaga ggacggcgca aaaaaagagc tgtatcaagt tgaatttggt agctatggga
    71761 aaaatatctc ccaagaaacc aagcaaaaca tcgaagcgga catgaaaaaa gaaggtgtcg
    71821 ctggtcttta ttttgtcgac catcaggctc ggatgtatcc aaatggcgtc ttttcttctc
    71881 attttattgg atacgcagtt ccagacaaag atgagaatgg cttagttggt aaactaggtt
    71941 tggagtctgc ttacaatgat atcttgagcg gtaaagatgg taagattatt tatcaaaaag
    72001 ataattttca aaatcctctg ccaggtacag tggcagaaga agaaaaagct gtcgacggac
    72061 aagatatcta cacgacttta gacagcagac tacaaagtta tttagaaaca ctgatggatc
    72121 aagtaaatga agaatatcag ccagaagaat tgacagcagt cctgatgaaa gcaaaaaccg
    72181 gagaaatctt ggcaatggga caacgaccta ctttcaatcc tgaaacaatg gaaggattga
    72241 ctggaaaaga tgctatctgg cgtaatttct tggtacaaga tagttatgaa ccaggatcaa
    72301 ccatgaaagt tttcactact gcagctgcta tcgaagaagg agaattcaac gagaatgaaa
    72361 ccttccaatc tggaaaaatc caagtagaag atgctacgat caatgaccat gactttggtg
    72421 aaaaaggtgt gctgaccatg cgtcaagctc tttcatggtc aagtaacgtc gggatggtca
    72481 tactggaaca gcgattaggc ggacgatggt ataactattt acaaaaactg ggatttggac
    72541 aaagtactca ttctggatta gatgatgaag taaacggtgc tttacctaca ttaaatattg
    72601 tcgaccgagc aatgagtgct tatggacaag cggtaggtgt gacgaatttc caaatgatga
    72661 aagctttcac ttctattgcg aataacggta caatgattca acctcgatat atcagcaaag
    72721 tggtggatcc ccaaactgga gaagagcgaa caactcaaac agaagttcta gggcaacctt
    72781 tttcaaaaga aacaacagaa aaagtccgtg aatatatgag agatgttgtc gaaagcgaaa
    72841 actacggtag tgcttatggt gtatatagtg tacctggata caatgtatcg gcaaaaaacg
    72901 gaacagccca aatcgcttct gatacagggg gatatcaaac aggagataca gcctacctgt
    72961 attcgattgt ggagatggtt ccttctgaag atccagacta tgtcttgtat ctaacaatga
    73021 aacaccctaa gacatatgat cgaatggcat tggctaaaat cgcaaatcca ttgatgaaac
    73081 gggcaatgga ttttaaagaa actgaagaag attcagatac agaaacaaaa acagaaaaag
    73141 tatccgtagc cgattatcgt aatcttgaag cggacgttgc agcagctgat gctcaaaaaa
    73201 gtggactgca acctgtagtg atcggtaatg gtaaaaaagt acaaaaacaa tctactgcca
    73261 acggtgatca gttgatctct ggagaaaaac tgatcctata tactggtggt gataagctga
    73321 tgcctgacgt aacaggatgg tcaaaagcag atatcatgaa gctggggaaa atcctcggta
    73381 ttgaagttag ttttgatgga gatggttatt gtgtcaaaca agaacttgct ccatatgaaa
    73441 aaatcactga agacaaactt agttttactt tagaagaata agaagagaaa taggagagaa
    73501 aagagcatgg aatggacaca agcattgatt cccattgtca gcagttgtgc aatgacaatt
    73561 gccgcaatgc cgctatttat tggttatttt caaatgaaaa agcaaggtca ggcgattcgt
    73621 gaggagggac caaagtggca caatagcaaa gccggaactc ctacaatggg cggcttagtg
    73681 tttctgatcg gctctatttt gacaggaatt tgggttggag cttggcaaaa acagctaact
    73741 cctactcttt tcatcttgct ttttgtatta gcgttatacg gagtaatcgg ctttcttgat
    73801 gattttataa aaatattcaa gaaaagaaac atgggactga actcgaagca gaaattgatt
    73861 ggacagattc tagggggaat catcttttat cttgtttacc gttctgaagg ttatccaggt
    73921 gtattgaatt tcttcggact agatctacct ttaggaatca tttatggtat tttcgctctc
    73981 ttctggcttg ttggattttc aaatgcagta aatctgacag atggcatcga tggattagtt
    74041 gctggcttag gaagtatctc ttttgcagct tacggtctaa ttgcttggca tcagcatcag
    74101 tatgatgtat taatcatttg cttaagtgtt ttaggaggtt tactgggttt cttcgtctac
    74161 aacagaaaac ctgccaagat ttttatggga gatgtcggtt cattagcgtt gggcggactt
    74221 ttagcggcaa tttctataat gctaaaccaa gaatggacat tgcttttgat tggtttgatc
    74281 tatgttatgg aaacagcaag tgtgatgctt caagttactt cctttaagct tacaggaaaa
    74341 cgaattttta aaatgtctcc aattcatcat cactttgaaa tgtgcggatg gtcggagtgg
    74401 aagattgata cagttttttg gttgatcagc attgtgactt cgttgatcac attatggatc
    74461 gtttggtagg agatgacggg aaatgaaaaa aatatcaaca tatgaaaata agaaagtgct
    74521 tgtcttagga ctcgcaaaaa gcggagtaag tgcggccaaa ttattacatg aattgggcgc
    74581 acttgtcacc gttaatgatg gaaaaccttt tgacgaaaat ccggaagcac aagagctgct
    74641 ttctttaggg ataaaagtaa taacaggtag tcatccaatc gaattgttgg atgaagaatt
    74701 ttcattgatg gtcaaaaatc cagggattcc ctacagtcat cctttagtag caaaagccca
    74761 agaaatgggt attccggtaa ttacggaagt tgagctagct tacgaagtag cggaatgccc
    74821 aattattggg attacaggaa cgaacggaaa aacaacgaca acaacgatga caggactact
    74881 actgaatgca ggagcagatc aaggaattgc acgtttagcg ggtaatattg ggtatccagc
    74941 aagtggtgta gcgcaagaag caaaatcaga agataaaatt gtgatggaat tgtctagctt
    75001 ccaactcatg ggaatcacag atttccgccc tcatatagcg gtgattacca atatctatga
    75061 agcacatatt gattatcatg gtacaagaaa agaatatgtc aaagctaaat ggaaccttca
    75121 aaaaaacatg acagaaaaag attatctgat ccttaactgg aatcagtcag aactacaaga
    75181 attagcgcaa agaacaaaag cacgcgtact gcctttctcg acaaaagaag tgttggaaga
    75241 cggtgtgtat gctgacgatt actcaatata ctataaaaaa gaaaaaataa tggaaatatc
    75301 tgaacttggc gtaccaggta agcataatgt agaaaatgca ctagctgcta tttctgtagc
    75361 aaaattgtat ggtatatcaa atgaagctat acgagaaacg ttgcattttt tccatggcgt
    75421 accacatcgt acgcaatatg taggagaaat ccaaggcaga aaattttata atgattcaaa
    75481 agcaaccaat attttagcga ctaaaatggc tctaagtgga tttgaaacgt ctaaggtcgt
    75541 gttattggct ggcggattag acagaggaaa tacatttgat gaattaattc cttcattaaa
    75601 aggaatcaaa gcaatggtcg tatttggcca aacaaaagaa aagttgatgg atgctggaaa
    75661 aaaagcaggc atcgagacaa tagtaacagc cgattctgta gaacaagcag ttcctttagc
    75721 attagaaaac agtacagatg gtgatgtagt tttattatca ccagcgaatg ccagctggga
    75781 tcaataccca aattttgaaa ctagaggaaa cagattcatg gaagctgtga accgattaaa
    75841 atagaaaaag tagaagcgag cgataaaaaa tgaaaatttt ggttacaggc ggaggaacag
    75901 ggggacatat ttatccagct cttgcctttg tcaattatgt aaagactcaa gaaccgaatg
    75961 ctgaattcat gtatgtggga gcaaaacgag gtctagaaaa caaaatcgtc cctgatacag
    76021 gaatgccttt ccataccttg gagattcaag gatttaaacg aaaaatttcc atgcataaca
    76081 tcaagacggt ccgattgttt cttaagagta tccgtcaagc aaaaaaaatc ctcagtgatt
    76141 ttcaaccaga tatcgttatc ggtactggag gatatgtttc cggagcggta gtgtacgctg
    76201 cagccaaaat gggtattcca acagttatcc atgaacaaaa cagtgtacca ggtgttacca
    76261 ataaattttt aagccgatat gtggatcgga tcgctctttc atttaaagat gcggcttcct
    76321 tttttcctga aaacaaagca gttcttgtag gtaatcctcg agcacaagaa gtagcagata
    76381 ctaaaaaatc agatattttg aaaacatttg atttggatcc agaaaagaaa acagtcttga
    76441 tttttggcgg tagtcaagga gcacttaaaa tcaatcaagc agtatcagtt tttttaagtg
    76501 aatatattcc ttcttcttac caagttttgt atgcttcagg agatagatat tatgaagaga
    76561 tcctaaataa aataaatcct gaaatacaaa atattagtgt ccagccttat atcaaaaata
    76621 tggcagaggt catggcttcc agtgatctgc tggtggggcg tgctggtgca acttcgatcg
    76681 cagaattcac agctctgggg ttaccggcag tgttggttcc cagcccatat gttacgaatg
    76741 atcatcagac taaaaatgca atgagtcttg ttcacgcagg agcagcaaaa atgattgcag
    76801 ataatgaact aacaggtgaa tcactaagtc agacagtcaa cgaaatcatg ggtgatgaag
    76861 aattgcaaaa gcaaatgtgc cgagcttcta aagaacaagg aatccctgat gcttcaaaac
    76921 gtctttatga cttagtaaaa caaataatca aaaaataaag gcggtggtct tagtgagtaa
    76981 gaaaaaagaa ccaaaaaaat tgtctcatcc gtcttctgat gaaaacaatt tgactccttg
    77041 gcaaaaggct aaccgaaaat atctggcaga acaagagatg aaaaaccaag agccaaaaaa
    77101 agaagacata acattagata cacccctttc agattctgaa gaagaaaaaa cggttcaaga
    77161 ggaacagcaa gaagaaagcc agtccgagaa aaaaatcgaa ttttttgaag agatcaagca
    77221 aaagaaaaac ggtggaccat ataacggttc ttttttgaac cggctgccca atttaaaaag
    77281 tcaacggcac aaagtactat ttcgtcggtt agctttgatc atcactgtat tgggtatacc
    77341 gctgattttt ttgatctatt atgtttctcc ttatagcaaa ttacaagcag ttgcagtatc
    77401 aggcaataag acagtcaaca gccaagaaat catctctgat acaaaattgt cattaggaga
    77461 aaatgtttgg ggacagtatt ttcatcgatc tacttatatc gatcgcctta aaaaagcaca
    77521 gccaagaatc gaaacagcaa atatacattt caaagggatg aatgaattcg atttggatgt
    77581 aacagaatat aaagagattg ctttgatcgc aaaaaacaat caatactacc cagtgattga
    77641 aaatggaaca gtattagatg aaaaagtagc aaatcctaca aaaaatctcc ctatcttgga
    77701 agagttcaaa gactctgcaa aaatcaaaga attaacaaag caatacaatc agttatcatc
    77761 agaattacaa aaagcaattt ctgaaatcaa gtacacgcca agagcaagca ataaaaatct
    77821 gatccaattg aatatgaatg acggcaatca agttattgta aatatcaata atcttgcgaa
    77881 ccaaatgaaa tattattcgc aagtagccaa agatatggat gaaaaaggtg tgatcgacat
    77941 ggaagttggt atcttctcgc atccttacgg tagcactcaa gaaacggaag aatcgggcca
    78001 atcggaagaa acaactgaaa gtgaagaaaa tgcggatcaa acaaacgaat caagtgaaga
    78061 ggcagaaacg caaaaagcac aaacacctgg atcgtctagc acaaacggat catctgattc
    78121 aaatgattca agtgaaacaa gtcaaataga agataatcca agtacaactc cgtcaagttt
    78181 aaatgaaaat tgataaaaca actttaaaaa gtaagaatgt actttctaaa aaccagtaat
    78241 ttatggtaga attggaagca gtgaatatat ctattaataa gtatatttta aaacggacgc
    78301 tagttgttcg cttgtatatg gaatagtaag taggagggaa tcccattcat ggcaaaaaca
    78361 ggaatgtatg taggccttga tattggaacg acatctgtta aagttgtcgt ggctgaatat
    78421 atcgacagcc aaatgaatat tattggagta ggaaacgcaa aatcagaagg gattaaccga
    78481 ggcattatcg ttgatatcga taaaacggtc caagcgatac aacgagcagt acgacaagca
    78541 gaagaaaaag caggtatcca gatcaaaggc gtaagtgtcg gtttgccagc aaacatgctt
    78601 gaagtagaaa attgccaggg aatgattgca gttaacggag attcaaagga aatcaccgat
    78661 gaagatgtca gaaatgtggc ttcagctgcc cttgttcgtt caatacctcc tgaacgtcaa
    78721 atcgtgtcaa tattgccaca agattttact gtcgatgggt tcgagggaat caaagatcct
    78781 cgtggaatga tcggcgtaag attagaaatg tacggtctgt tgtttacagg acctaaaacg
    78841 atcatccata acatacgtaa atgcgtggaa aatgccggat taatcgtcaa cgaaatggtg
    78901 attactcctt tagcgctgac tgaatcaatt ctttctgacg gagaaaaaga ttttggtacg
    78961 atcgtcattg atatgggtgg cggccaaaca acaacggctg ttatgcatga taagcaattg
    79021 aagtttacca atcttgacca agaaggtggc gaatttgtca ctaaagatat ctctattgta
    79081 ttaaatacgt catttaacaa tgcagaagcg ttgaaaataa actacgggga tgcataccca
    79141 gaacggacat caccagatga agaatttcca gtagatgtga ttggtcagtc agaaccagtg
    79201 aaagtcgatg aacgctactt gtcagaaatt atttcagctc gaatggaaca gattttcaac
    79261 aaagcaaaag atgcattgga ccaaatcgaa gcattagagt tgccaggtgg cgtcgtattg
    79321 acaggcggag ctgctagtct tcctggtgtt gtggacttgg cacaagaaat cttcggcgta
    79381 aatgtaaaat tgtatgttcc aaatcacatg gggctgagaa acccggtatt tacaaatgta
    79441 ataagtatcg tggattattc agcaaacttg agtgaagtgt atcaattggc aaaaattgct
    79501 gttacgggtg aaacctcagc agctcgtcaa atggtagttg aacaagaaac aacaaacaca
    79561 tatgagagct atgaagcacc tgaagaaaca atctacgatg agccggaacc aaaagagtcc
    79621 ggagaaaatg taaaaaataa aatcaagggt ttctttacaa atatttttga ttaattcatc
    79681 ctgggaggaa taagatatgg aattctcaat tgataataat atcaatgacg gcgcagtaat
    79741 caaagtcatc ggagtcggtg gtggcggtgg aaatgctgta aaccgaatga ttgaagaaaa
    79801 cgttaaaggt gttgaattca ttacagccaa cacagacgtg caagcattaa aaaattcaaa
    79861 agctgaaaca gttatccaat taggccccaa atatactcgt ggtttaggtg ctggttctca
    79921 gccagaagtt ggacaaaaag cggcagaaga aagtgaacaa tccttacgcg aagctttaga
    79981 cggtgcagat atgattttca tcactgcagg aatgggtgga ggaaccggta caggtgctgc
    80041 accaatcgtt gcaggtatcg cgaaagaatt gggcgcattg actgttggtg tcgtgacacg
    80101 tccattcact tttgaaggac cgaaacgtgg ccgttttgct gcagaaggta tcgctcgttt
    80161 gaaagaaaat gtggatacat tattaattat ctcaaataac cgtttattag aagttgtaga
    80221 taaaaagaca ccgatgcttg aagctttccg tgaagcagat aatgttttac gacaaggtgt
    80281 acaaggtatc tctgatttga tcacagcgcc aggttacgtt aaccttgact ttgctgacgt
    80341 gaaaacagtg atggaaaacc aaggtacagc attgatgggg atcggtgtgg ctagtggaga
    80401 agatcgcgta gtagaagcaa cgaaaaaagc aatctcctct ccattgcttg aaacatctat
    80461 tgatggagct gaacaagtac ttcttaacat cacaggcggt ttggatatga cattgttcga
    80521 agcacaagat gcttcagata tcgtagcaaa tgcagcaact ggcgatgtaa atatcattct
    80581 aggtacatct atcaatgaag aaatgggcga tgaaatccgt gtgacggtca tcgcaacagg
    80641 tatcgatgaa tcaaaaaagg aacgcaagtc tagccgacca gctagacaag cacaaatgca
    80701 atcacctgct caaaagactg tattagatat ggatcaagct aaaccaactt cttcagaaga
    80761 agaaaacagt tttggcgatt gggatatcag acgtgaacaa aatgttcgtc ctagagttga
    80821 tgattccaat tttgacaata tcgaaaaaaa agaatttgat acattcaacc gcgaagaaac
    80881 aaaatctcat ggcgatgatg aattaaatac gccaccgttt ttccgtcgta aaaaataaag
    80941 gagaggaaga gcacatgatt gctgacaact tgcagaaaat caagcaagaa attcaggatt
    81001 cgtgtgctct tgttcatcgt gatccgaaag aagttacatt agtagctgta acaaaatctg
    81061 ttgaatccgc tcaagctgaa gaattggtta gagctggcgt tatgaattgt gcagaaaacc
    81121 gagtagataa attattagaa aagaaacaag atttatttga ttttcctgaa ataaagtggc
    81181 atttaattgg taatttacag cgcagaaagg taaaattgat tgtaaatgaa attgattact
    81241 ttcatgcgtt ggacagttta agattagcag aagaaatcca gaagcggtta gagaaagaat
    81301 tatcctgttt tattgaagta aatgtttctg gtgaggaatc aaaacatgga atttcaccag
    81361 atgagctttt accgtttgtg gaatctttag ctgcctgcac aaaaatcaag gttgttgggt
    81421 tgatgactat ggcgcctaaa gaagcttctg atgagcaaat ccataaagtg tttggtacgc
    81481 tgaaagaatt gcgtgatctt gttcaagcaa aaggttatgc ccatgcacca tgtagtgaat
    81541 taagtatggg gatgagtcaa gactttcaaa ttgcgattga agaaggtgca acatttatcc
    81601 gtgtaggaac agcgctattc aaagacgaat aaaggaggaa caaaaatgcc gcttttaaac
    81661 agaggagcaa ttgctaattt tttcggatta gctgatgagg aagagtacga ataccaagat
    81721 tatcctgaaa caacaaaaaa ggctgtacag ccaactaaaa aaaatacaac acagaatcaa
    81781 tcaactcatt ctgtatctgc tgcgaatact catgaaaaac aaaaaacggc gacttcacgt
    81841 cctgtaacgc atgaaagtat tcagccggct gaaaagaaaa aagtaattga actacatcaa
    81901 tcacctacaa aaactgtagc gaaagaaaga acagcttcaa gaacctctac acaacctaaa
    81961 gcaaatcttg ctggaaaaat cactgtaatg gaaccaagag cttattctga agctatggcg
    82021 attgctaaac gaatcattgc tggagaagca accttagtga attttcattt agtagaagaa
    82081 aaacaagcga gaagaatcgt tgattttcta acaggaacag tttatgcact agatggagat
    82141 atcaaacgtg ttggagatga gatttttctt tgtacaccga ctgggatgga gattgacagt
    82201 tcaaccgctc aatcttttgc agatgcgaat ttgtttgatc tgtaagagga ggaactacga
    82261 tttatactat tgcactatta cttcatttac ttcaacgggc agcctatttc tatagcatta
    82321 tcttgatggt atatgcattg ctctcttggt ttccaggcgg gtatcaatca gggctaggac
    82381 gattattagc taagatctgc gaaccgtata tcagtttgtt cgatcgtctg cctttgagga
    82441 ttggaccctt ggatttcaca attttagggg caatcctgat cttgaactta gccgtacaag
    82501 gtttagcaca gatcgttata tggataactg tccatttagt ttattgattg aagcaacaaa
    82561 atctttattc tcagaataga taggagggga aaattttgga tgcaaatgta taccagcact
    82621 ttcgggcaga tgaacgtaca tttatcgatt cggtaggcga ttggattgaa caggttcaag
    82681 gacaatatgc accctatctg accgaatttc ttgacccaag acaatcctat attttagaaa
    82741 cactgattcg acaaagcagt gatttgcgtt tcatgtttta tggcggatat gaacaggcag
    82801 aaagaaaaag atgtttgatt ttccccgatt attacgaacc tgtagaggaa gattttgaaa
    82861 cagcattgta tgaaatccat tatcctagca aatttgccag cctaagtcat gggaaaatcc
    82921 ttggaacttt agtgggaacg gggatcaaac gctcttactt tggcgatatc atctcggacg
    82981 gtgatcgctg gcaagtattt attgcaaatg aggtggatca ttttgttgct gcacaagtaa
    83041 caaagattgg aaaggttgct gtacgcttgg aaccaatcaa atacacagaa atgattatgc
    83101 cgaaagatag ctggacacaa gaaagaggaa cagttagttc catgcgacta gacagtgtga
    83161 tctctgagat ttataatatt tccagacagc ggtccaagca gctgatcgaa gctggtaaag
    83221 taaaagtgaa ctggacagaa aatacaagac ctgattttgt acttgaacta ttagatatta
    83281 tttcagtcag aggttatggc agagtccaga tccaagaact tgaggggaag acgaaaaaag
    83341 aaaagtacag ggtactgttc ggtgtattac gtaaataaaa taaaaaggtg gtttactcat
    83401 ggctttaact ccattagata tccaaaataa gacattcccg acaaaaatgc ggggatataa
    83461 tcaagacgaa gtagatgatt tcttagattt agttgttcgt gactatgagg aattgactca
    83521 aagaaatcga gaattagaaa aagcagtcaa acattctgaa gaaaaacttg agtacttcaa
    83581 tgaattaaaa gatgctttga accaatcaat catcgtagct caagatacgg ctgataaagt
    83641 aaaaacaagt gcaagcaagg aatccgaagt gatcgttact tctgcacaaa acaaagcaga
    83701 tgagttagtt gcgaacgctg aaaaacgtgc acatcaatta acaactgatg cagaagaaaa
    83761 agcaagaaaa atcttgacag atgcaacaga aaaagcaaga cagcttgcga cagaaactga
    83821 agatcttaag aaaaaaacac gagtattcca tcaacgtatc agcttgatgc ttgagtctca
    83881 attagaacaa gtgaaaagtc cggaatggga tgaaatttta caaccattct ccagctacgt
    83941 gactgacagc catgaagtga tcaaagaagt tttagcaaaa cagcttgaca atgaaaatga
    84001 tactgatgta aactcagaca caggagtcat agaagcacct atgacggcat ttgatacaca
    84061 ggctattgat atctcgatcg tgcctggtga aaatgaaaac gagtagaaca gaaaagcact
    84121 ttgttattct taaagcgagc cgatgagagt ggaagttcgg taagacactg catggtgaaa
    84181 gatcctctac ttgagacctg cttttccgaa attagagtaa gaagcagcgg ctgacctcgt
    84241 tattggtcac agagagtttg cactagatag atgcaactaa atttgggtgg taccacggca
    84301 tgttcgtccc ttgcggctga acatgctttt ttgtgtccgg aaaacaaata aaggagtgta
    84361 tggttgaatg aaaatgaaag aaaccttgca attaggaaaa acaaaattcc ctatgcgtgg
    84421 taatttgcca aaccgagaag gcgagtggca aaaagaatgg gaagaaaatc gaatctacca
    84481 aaaaagacaa gagttgaacg aaggaaaacc tagctttgtt cttcatgatg gacctccata
    84541 tgccaatggt aatatccatt taggacattc attgaataaa atcagtaaag acattatcat
    84601 tcgttcaaaa tcaatgtctg gctatcgtgc accatatgtt ccaggatggg atacacatgg
    84661 attgccgatt gaacaagtat tggctaataa aggcgtgaaa cgtaaagaaa tgtccatggc
    84721 agaatacttg gaaaaatgtc gtgaatatgc actttcccaa gtagataagc aacgagcaga
    84781 tttcaaacgc ctaggagttg caggagattg ggaacatcct tatgttacat tagatccttc
    84841 ttatgaagct gcacaagtac gcgtatttgg taaaatggct gaaaaaggct atatttacaa
    84901 aggattgaaa ccaatttact ggtctccgtc aagtgagtct tctttagcgg aagctgaaat
    84961 tgaatataaa gatgtaaaat ctccatctat ttttgtagct ttcaatgtca aagacggcaa
    85021 agatattctt gatgaagata cagcttttgt catttggacg acaacaccat ggacattgcc
    85081 agcaaaccaa ggtattgctg taaatccaaa atttacgtat gtattggtgg aagccgatgg
    85141 ccgtaagttt gtcgtagcga aagatttgct agaaacagtc caatcagaaa tcggttggga
    85201 aaatgtaaca gttctaaaag aatttgctgg acaagaaatg gaatatatga ctgcaagtca
    85261 tccattctat gaacgtgaat cacttgtgat tttaggcgat catgttactt tagacgctgg
    85321 tactggatta gtccatactg ctccaggaca tggggaagat gactatatcg ctggtagcaa
    85381 atataaacta tctgtagtgt cacctgtaga ctcaaaagga ttgtttacag aagaagcacc
    85441 tggatttgaa ggtatctttt atgacaaagc aaatcctatg atcactgatc tattaaaaga
    85501 aaaaggagct ttgctgaagc tagatttctt tactcatagt tatcctcacg attggcggac
    85561 gaaaaagcct gtgatctatc gggcaactcc acaatggttt gcatctatcg ataaattccg
    85621 tcagaatatt ttggatgaag tagaaaaagt agagtgggtt atcccatggg gtaaaacacg
    85681 tttgtataat atgatccgtg accgcggcga ttgggtaatt tcccgtcagc gtgcatgggg
    85741 agtaccactt ccgattttct atgctgaaaa tggagaagca atcatcacac cagaaacaat
    85801 cgaacatgtg gcaaaattat ttgctgaaca tggatcaatt atctggtttg aaaaagaagc
    85861 gaaagaactt cttcctgaag gatttaccca cccaggttct cctaatggcg aatttacaaa
    85921 agaaaaagat atcatggatg tctggtttga ttctggttct tctcatgaag ctgttttacg
    85981 tcagcgacca gaattatcat tcccagcaga tatgtattta gaaggatcag accaatatcg
    86041 tggctggttc aactcaagta tcacgactag tgtggctatc aatggtgtag caccatacaa
    86101 atcagttatt tctcaaggat ttgctttaga tggtgaaggc cgtaagatga gtaaatcttt
    86161 aggaaacatc attgcaccag aaaaagtgat caaacaattt ggtgcagata tccttcgctt
    86221 atgggtatct agtgtagatt atgaagcaga tgttcgtgtt tctatggata tcttaagtca
    86281 agtggcagaa gtatatcgta aaatcagaaa tacgatgcgt ttcttattag cgaatacaga
    86341 agatttcgat cctaaaaaag atactgtttc gtacaatgat ttacgttctg tagacaaata
    86401 tatgaccgtt cgattgaacc aagtaatcaa agaaatccga gaagaagggt acgacaaata
    86461 taacttcatg catatttacc gtacagttat gaacttcttg acggttgatc tatcgtcatt
    86521 ctacctggat tttgctaaag atgtagttta tattgaagca gaagatgatt atcaacgtcg
    86581 ttgcatgcaa actgttttct atcaaacagc tgtagctttg acaaaattat tgactcctat
    86641 cattccgcat acagcagaag aaatctggtc attcctgaaa gaagaagagg agtacgttca
    86701 gttaagcgag tttcctggtt atgaagagtt tgcaaaccaa gaagaattga tggatacctg
    86761 gactgctttc atggacttcc gtgataatgt cttaaaagca ttggaagaag caagaaacag
    86821 caaattgatt ggtaaatctt tggaagcaaa actaactgtt tatccaaaac aacaagttcg
    86881 tgaaatgtta aaagctttgg atgcagatat tgcacaatta ttgatcgttt caccagacta
    86941 ctttgaagtt atggcagcag atgaacaagt gccagataac gctatggtat ttgatgatgt
    87001 tgctattaca gtggaaaaag cagacgggga aacttgcgat cgctgtcgcc aagttcgtaa
    87061 agatgtagga gtagatgaaa aacttcctca tctatgcggt cgttgtgcaa aaatcgttga
    87121 agacttctat cctgaagctg tagctgaagg attcgaagaa aagtaaaagg gaaaaggggg
    87181 tgtgacatgt tctttgtcac accccctttt gttatcaatc gtttaatttt ccacgttcac
    87241 gataccagtt atctggttgc cagatccatt cgaatccgtc tttttcaagt agttcgaaag
    87301 ctgctttagg tcccattgat ccagctttgt agttgggaaa atccggttgt gtttggtccc
    87361 aagcttttcg aatgacatcc acgatatgcc aagattgagc gacttcttcc caatgagaga
    87421 agttcgttcc atcaccattc aacgcatcta atagtaattt ttcgtaagct tctgggctat
    87481 tttcaaccat ttctgcacta tttctaaatt ccaatttgat tggttccgtt tcaaatcctt
    87541 gccctacttc tttcccattc aaggataaag aaaatccttc agttggctga atatagatcg
    87601 ttagaacatt aggtggaagt gtcgtatcgt cacatggttc atcgacagat gttttgaata
    87661 cattaacagg aacttgtttg aatacgatgt tgatccgagt ccctttttca gtcaaacgtt
    87721 tgcctgtacg tacatagaaa ggaacacctg accaacggaa gttatcgata gtgaatttgc
    87781 ctgcgacgaa tgtttcagtc tctgattgag catccacttt gtcttcttcg cgatagccct
    87841 tgaattgtac accatccaat tcaccttgag catattgccc gcgaacgaag ttttctaaca
    87901 cttcttcttc gttataaatg cggatagctt tcaatgcttt gatcttttct gtacggattt
    87961 ctttttcgga aaatgctact ggcggttcca ttgcaagtaa tgctacaacc tgtaaaatat
    88021 gattttgcac catgtcttta agtgcgccac tatgatcgta gtagcccccg cgatcttcta
    88081 caccgaggtt ttcgccaaaa gtaatttgga tattgtcgat atagcgattg ttccattgag
    88141 cctcaaaaat attattggca aaacggatag ctgagatgtt ttggatcatt tcttttccta
    88201 aataatgatc aatcctgaaa atatcttgtt ctgggaatac ttctctgatt tgctggttca
    88261 attcaaaagc agactggtaa tcagaaccga acggtttttc tatgatcaaa cgattgaagc
    88321 cattttttgt cataatattt tgtgatttca aatgagaaac gatcgttcca aaaaattgag
    88381 gtgccatagc caaataatag acacggttac cgtttaattg atagtgtcca tctaatcggt
    88441 cagacaattc ttttaatgtc tgataatgcg ttgaatcatt tacatcatgg gattgatagt
    88501 aaaaatggct tgcaaaggta gttgcttctt catctgaagg attcaatgca cggattgttt
    88561 ctttgacaac ttcacggtag tgctcatcgc tccagggacg gcgagcggtt ccgattacag
    88621 caaaatgctc gcctaaattt ccttttctgt aaaggcggaa caaagacggg tacaatttac
    88681 gctgggctag gtcacctgta ccgccgaata tcgtaaacaa tacatttttt tcattagaca
    88741 tgtggataca ctcctttata ttttatcgac aaaaatcgtg ctggccgcct taaagctgac
    88801 ggggattttc tttctatcat tttggatagt gatcggtcct tcataagctg cgatgtcaac
    88861 gatctcatat tcttcgttga tattcaggtc aatagtgact aagtaatcca acaattcttt
    88921 ttcatcaagc actcgtgcga tacgcacttt tgtaccgaca ggataggact ccaatgtttg
    88981 tcggcgttct tcatggacaa cttcctcttt tttaggaatc gatcctccat gaggacaatg
    89041 ttcaggatga ttcaaatagt tttctaaatg atctgcaaga tgttccgaag tcacatgttc
    89101 caatacttca gcatcatcat ggacttcatt ccagctatag tctaaatgtt caactaaaaa
    89161 aacttcccat aagcgatgtt tgcgaatcag actgctagct ttttgcagtc cgcttgcagt
    89221 caattgtaca ccttgataag gggaatgttc tacaagctct tctttcacaa gtttagaaat
    89281 catttcactg acagatgcag ctgagacatc tagactagat acgatttgtt tattgttgac
    89341 tttcgttgta tctccaccta gttctaaaat gattttcaaa taatcttctc gatttggtgt
    89401 catattcgat catcctttca tggtaaaaaa ccatcattag taagtgtaac aaaaaaaacg
    89461 ttaattgact atttttctaa ataaaaaaca aaggattttt agacgttttt acaacaaaaa
    89521 acgaaagatg atagagaaga ctcattcttt cgtttataaa ggatatatat atacattggg
    89581 agggatctat ttttcagata acaacttata tgaagtgtct gcagattcgt cataaagagt
    89641 ttgttcggga taagaaaaga taagataaaa accatactga tcacgtacga ctaaacgata
    89701 ttcatctatt tctaccacag atgtgccaag agactgaccg ttgatcaaaa acgtgtaatc
    89761 agatgtaatc tgtaattgtt gtttttgctg gctatcacta aagaaccatg taccttgata
    89821 ctttgagagt ccttctgact tttccgcttg ttttttcttt ttcaagtaaa taaaacttga
    89881 gagaacggta atgagtgaac caagaaaata gatcattttt tttatcacca aatcactcct
    89941 ttatgagaaa ataatcgcat agacatattc tttcttcttt atttcattgt accgaataaa
    90001 agctaaaatt tctctaagac tttcgactgg tttttttact gtttttctac aaattatgat
    90061 aggatacata gtaggaagaa aggtgacata atttgaagta tcagcaagtg gaaatagctt
    90121 tttttattga ccgcccgaat cggtttatag cacattgtat gaaaaaagac ggagaaataa
    90181 ttattgcgca tgtcaaaaat acaggtagag gaaaagaggt cctgatacca ggcgctgaag
    90241 tggctattgt atttgctcct ggacctaaac ggaaaaccgc ctatgatctg attgctgtaa
    90301 aaaagaaggg tgactggttt aatatcgata gtcaattgcc taatcggctg gcaattgatg
    90361 gtattttaga cggcacgatc cttttaccag gattaactgg tccatttacg gtatacaagc
    90421 gtgaagtaac ttatcttcac tccaaatttg atatttattt agagacggtg gtcggacaaa
    90481 aagcatttgt tgaagtcaaa ggtatgacac ttgaaaataa agcgattggt gcttttcctg
    90541 atgcgcccac actcagagga ttgaaacata taggagaatt aacgtatgcc gcacaagatg
    90601 gttatgccgc ttatgtcttg tttattgctc agttcgaaca tcttcatctg gcaacgatcc
    90661 atgaagaaat gcaaccagca ctagctgata tggttcgaca tgcgcagcaa agcggagttc
    90721 aaatcctcgc ctataattgt caagtatcgg cagatgaggt aactgtcaag caggccattc
    90781 catttgatgt agatatccct tttgaagatc ctatacaata gaaagaaggt aaaataatga
    90841 tcaacttagg tattatcgga acaaattgga ttactcatca atttgtccaa gcagctttaa
    90901 gcacaaaaaa atatgaattg acagcggtat attctagaca tttggaaaaa gcacaggaat
    90961 ttggttctca ttatgaaggg gatatcgaat tcgcaacaga tttgaagact tttttcaata
    91021 ttactcatat ggacaccatt tatattgctt ctccgaatag tcttcacttt agtcaagcta
    91081 agcaagcaat tttggcagga aaaaatgtga tagttgaaaa gccagctttt tcgacgccaa
    91141 aagaaatgga tgaaatcatc catttggcaa atgaacacaa tgtgtatttt tttgaagcag
    91201 caagaaatat ccatgaatca ggttttaaaa agattgccga ttacttacca ttaaaagacg
    91261 agataatcgg tgcaaatttc agctatatga aatattcttc gcgctatgac caagtattag
    91321 atggagaaga accgaatatc ttctcgactc atttctcagg tggtgcattg atggatttag
    91381 gtgtttacct tgtatatgca gctgttgctt ggtttggaat gccaaaagaa gtccattatt
    91441 tcccaagaaa gatttctact ggtgtagatg gtcttggatg ggggatctta cgttatgaga
    91501 attttgatgt atcgatccag ccaggtaaaa tcggtgattc ttatttaccg tcagagattt
    91561 attttgactc aggaacgcta gtgatgaatg gagtcaatgc aattgaacaa gcacaatttc
    91621 atgatcgcgc gcataaagaa atcattgatt tggcggtaac agctgcagaa aatccaatgg
    91681 cagatgaagc agaggatttt gccaatgtca tggctcatcc gaaagatcca gcttggggat
    91741 tgttgtatga agagtggatc gagctagcta gaaatgtgaa ccaagtgatt tatgatttaa
    91801 gaaaaaatgg cggcatcaaa ttcgatgcag ataatgagga agatttttaa tgaacgaatt
    91861 tgtacaagta tttcccgaaa attggcagaa aaaatatgag gataaagggt ttatctcacc
    91921 ttctcttatt caacgagcag tttttcagcc actaagtaat aaacaaagta tagtagcggt
    91981 ttctcctaca ggttcaggta aaacactggc ttatctttgg cctctactac taaacgtgga
    92041 accaggtgaa gcaagtgcgt tggtcatttt tgcttcttct caagaactag ctattcaagt
    92101 agcagatgtt gcacgtgaat ggggaaaaga caaagagttg aaagtccaat ctttggtggg
    92161 cggagccaac gtcaaacgtc agattgaggg tttgaaggaa aaaccagaaa tcttgattgg
    92221 tacaccagga agaatattgg aattgatgaa agcaaaaaaa ataaaagcac atcaagtgaa
    92281 aacaatggta tttgatgaag cagaccagct atttgacgct gggaacagcc aaatcattga
    92341 tcaaattctt catcaagctc caacagagta tcagctagct tttttttcag cgacagcaga
    92401 tcgttccctt gaaagcattg aaaaaatcac tgggaaaacg tttgagacaa ttgacgtgac
    92461 agcagaggac gattctcgaa aaggactgag acattacttt ttacgtgtac cgatacgaaa
    92521 gaaagatgac tacttgagac gtctcacgca tatagaagat tttcatggtc tgattttctt
    92581 taatcaactg actgaattag gcgtaatgga agaaaaattg ctgtatagag gtgtaccggt
    92641 aggaagtctg gcctctgatc agaacaagct actccgaaaa gcagcgctag aacaattcaa
    92701 aaagaaaatg gtcagagcac ttttgaccac agatgtggcg gctagaggtt tagatattac
    92761 aggtcttcct tatgtagtga atgcagaagt tccgttaagt gaagaagctt atttgcatcg
    92821 ttcgggaaga actggtcgaa tgggtaatga aggaatagta attacactcg tacaagataa
    92881 taatctaaga gatttaaaac ggcttttacg tccatcaaat ataacacttg aagagatttt
    92941 tttatacgga ggtaagctgc aaactgagca gccaaccaaa gaagatgcag ctcctgcagt
    93001 aaataaaaag tatgcgaaaa ctttttctgg aaaggagcag aacccagaat ataacaaaga
    93061 aaaaataaga aaaaaagtta aaaataaatc aaaaaaagag agaaataaag gagcaaggcg
    93121 caaataagcg cctttttatg ctatgattat gaaaacggga acaaaattgg ttgccagagg
    93181 aggggttatg tgaaagaacg tcaagaagag atcttgcagc tattgacaga aaaccaacaa
    93241 ggattgacag caagtgatgt agctgagcgc ttaactatcg atcgaagcaa tgcaagccga
    93301 tatttaagtg aactatacaa ggcccatcat attgtaaaaa ccgctggccg accagtcgtt
    93361 tactctttgc caacggaaaa gtcaaaatct gatgaagtgc atgtcgactc ttctacgcaa
    93421 gtgacttttg aaacactcgt tggagaaaat gattctttaa aagtcagcat acagcaggca
    93481 aaagctgcga ttctttatcc accgcggggg ttgcatacga ttatttttgg agaaactggt
    93541 acagggaagt cgatgtttgc tgaatgtatg tatcattttg caatcgattc agagatgctt
    93601 tctgcagatg cgccatttgt ttctttcaat tgtgcggatt atgctcaaaa tccgcaactg
    93661 ctctttggac acatctttgg gatcaaaaaa ggggcttaca caggagcagc tcaagatagt
    93721 cccggtctaa tagccaaagc agacggcggt atattgtttt tagatgagat ccaccgttta
    93781 ccgccggaag ggcaggaaat gctgtttact ttcattgata aaggtattta ccgtccgctt
    93841 ggagaaagtg cacaagtaca tgaagcatct gttcagatca ttggggcgac gactgaatct
    93901 tcagaaagtt tcttatctac gttcaatcga cggattccta tggcaatcac cttgccgaat
    93961 cttgcttcac gttcactgga tgagcgttat gaaatcatct ctctttttat caaacaagaa
    94021 gcgaatcgtt tgaatcaacg tatcgatatc gaaaaagaag cgattcttgc cttcatgctt
    94081 tatgatgcgg aaggaaatat cggtcaagtg aaacgagact tgaaactagt ctgtgcaaaa
    94141 tcttttttac attaccgtac tcatcatgag aaaaagctaa tcattcgaaa agaagaactt
    94201 tcccttcaag tacaaaaagg attactgaaa atcaaggaaa taccggaacg tctcgaccga
    94261 ttcatggaca gcaaaagtca gtatttgaca tttgaaccag gatttgctga tgttgtctgg
    94321 tcacaagatc cagaacgaaa catgcaggtc tataacgata ttgaggaaaa agtcacttct
    94381 ctttccgaaa ccggtgtgga gaacattgat ctagagaccc tcatttcaaa agatgtggat
    94441 gcttatttcc agacatatgt caaagaattg actaaaagtt cgattcccaa agatcttttg
    94501 ccagatgaaa tctggcggtt aaccaatcaa ttgtatgatg tggctgaaaa acggttagac
    94561 cgtatttata atgaaaaagc tcgttttgct tttgctttgc atctgcaaag tacattggat
    94621 cgtgtgagag aaggtcatat gatcgtccac ccagatttga ataacgtacg gaaaaatttt
    94681 aagagggaat tccaggtagc aatcgatctt tcaacgatca ttgaagaaga agaacaagtt
    94741 gagattcctt ttgatgaaat cggctttatt agtatgtttc tttctatcaa tctaggagaa
    94801 agtgagcctg ttaaagaaaa caaagtagat gttctcatat tgatgcacgg cagacagact
    94861 gcttcaagca tgttggagat ggcacaagaa ctattaggaa caaaagtagg gaaagctttt
    94921 aatatgccac tggttatgga agttcaaaca atgtatgaag aagtgttaca atatgtcaaa
    94981 aactatcaag agcatttgac gaatggccta ctcttgttaa cagatatggg atcattaaac
    95041 accttcagtg atttgattta tgaagagact ggcattcgga cgaaagctat cacgatgaca
    95101 agtaccatga ttgtgataga agcgatacgg atggcaagtg ttggaagaag cttagaagat
    95161 atttatcaaa atatccagct ttcctttgaa tcgatcgtac gtgatcagtt caaagcggaa
    95221 aagtctgaaa ctttacagcg taaaaaagcc gtaatcgtga catgctttac tggtgaagga
    95281 gtagctgcca aattatatca gcgtattatg ccagtgatta atcaaaataa ggtagaactg
    95341 atccagatgc agtttttaga acgtgaatca ttcaaaaaac acatcgatgc cttattggaa
    95401 gaatatgaga tcaaagcaat tgcgggaaca gttgatgttg attatcaaaa tatcccattt
    95461 ttttcagcct atgacgtttt tgatgatgaa aaactaaata ttttaaagcg tatagctgat
    95521 gatgagatcc cgacagctaa aattgtcgat tcacttgctg gatcattaag cgatgtcttt
    95581 tctattgatg aactgatccg ttcattgcaa aagacagttc agcagatcca gaccgatttg
    95641 tcgattatcg tggaacctgg agtagatact ggaatcgtta tccatttagc cttcatgtta
    95701 gacggattga tcaaacaaga aagccatcgg caatttgaga aactgtctat tttctcaaaa
    95761 atttatcgtt tggaaatgga tatcgttcgg gcaaacttga tggcactaga acgaaaatat
    95821 caggtaaaaa ttccagaaga tgatatcgca tttcttacac aaatgtttct tgaaaataaa
    95881 ataaaaaaat gaacacacta atcatactgt ttacacacta aacatctgtg tgcgcactat
    95941 aaaaaagagc taaaaagcat gaaaacgcta tttcagctct tttttttatt ggcatgcttc
    96001 ttgcgttata taaaagtgta aaaaaataaa aggagtgtta aaaatggcaa aaaaaacaat
    96061 catgttagtt tgttcagcag ggatgagtac gagtttattg gtcacaaaaa tgcaaaaagc
    96121 agcagaagaa aaagggatgg aagccgatat ttttgcggtc tctgcctcag atgcagataa
    96181 taatttagaa tctaagaatg tcgatgtatt attattaggt cctcaagtac gtttcatgaa
    96241 agctcaattt gaacaaaaac ttgcgcctaa aggtattcca ttagatgtca tcaatatgca
    96301 agactatggc atgatgaacg gagaaaaagt tcttgcacaa gcagaaaatc taatgaaata
    96361 aatcaagtat gcagttgggg gtataacaac gtggaagatc aacaaaatct agaagccatc
    96421 atggggttaa tcatgtttgg cggtaatgca aagagcgatg caatggaagc gattgcggct
    96481 gctaaaatcg gtgattttga gttagcggat aggaaaatcg ctgatgcaga agaatcactt
    96541 atacaagcgc atcatgcaca aacagacatg ttgacacaag aagcacaagg aaatcatatg
    96601 caagtgacat tattgactgt tcacagtcaa gatcacttaa tgacatctat tgcgtttact
    96661 gatttagcaa aagaaatcat cgaactttat cgtcgcgtat acagcgaata aatacaactt
    96721 cttttttgaa cggaagaaga aaaatttttg aacggatttt ttattttgtt ataaggagaa
    96781 ctctctttat ataaaaaact tataggagga agtaaaaaat gaacggacta tcaagttgga
    96841 tggagaaata catcctgccg gttgccgcaa aaatcggagc gcaaaaacat ttagttgctt
    96901 tgcgtgatgc ttttatcggc atactgcccg caacaatggc gggatcagtc gctgtaatga
    96961 tcaatgcaat tattcgtgat ttaccgcaac agtttaacag tacctatgct gacagtttag
    97021 cagcagacaa aggaatcttt gccgtattga atgtcatcat cggaatcaat ggttttgtct
    97081 ggaacggtac actagcaatc gccggattga tctttgcctt ttcttggggg tataatcttg
    97141 cccgagctta tggagtaaat gatctagctg gggggatcgt tggtttagct accttgattc
    97201 aaggtatttc ctttggtcca gcattatcag cagcattaga tgtgaatgtt ccttcaaatg
    97261 tcgcagatgc aatcaatagt tctgagattg gtgcaacgat tgcagatggc acactgtcag
    97321 ttggattatg gggttggcta aaactagatc atttgaatgg aaacgcttat tttacagtta
    97381 tgatcatggg agctatttca gtcattattt tttgtaaatt gatgcttgcc aacattacga
    97441 tcaaaatgcc tgattcagta ccaccagcag tatctaaagc atttgcagcc attttaccag
    97501 cgacgatcgc tttatacgtc attgctatta tcaattatat cgtaggtcgt gtagctgatg
    97561 ggcaactgat cattgatttg gtgcaaaaat atattgcaga accattccta ggattatctc
    97621 aagggttggg agcagtattg atcgtcacag tatttgttca agtattctgg ttctttggta
    97681 ttcatggatc aaatgtattg gcaccagtgc tagagggaat ctggggacaa gcacaactaa
    97741 tcaatattga tattttccaa aaaggttata acggaaaaac tggtacacca gccgtgttag
    97801 atgcaatcga agatggaaaa gcttatatgt gggtccgcgg atcgttcgat gcttttgcat
    97861 ggtttggcgg atcaggcgga acgatcgtct tactgatagc tatcttgatt ttctctaaac
    97921 gtgcagatta tctaacagta gctaaattat ccattgggcc tggtatcttc aatatcaacg
    97981 aaccagtgat gtttggtctg ccaatcgtat tgaatgcaat catgtttatc ccattcttga
    98041 ttgctccagt agtagcaaca gcaattggat ggacagctac ttatcttggc ctagtcgctc
    98101 cggtatcgca gcaagtcaca tgggtagtac cgccgttctt attgtcattc ttagcaacag
    98161 gagcggattg gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact ttcttgattt
    98221 gggcaccatt tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa caacaataag
    98281 atgaacgtga ggagcgggac gctttaagtt tcgttcctca taatgaattt tcgaggagga
    98341 actgaaatga ttacaattgc cagtgcaatg caacaagatg aaatcaaaga attgttagaa
    98401 ggatacaatg aagaaggaat gacttttaca tttaaagaaa aacaaggaat caagctgttc
    98461 tttgaaacaa gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga actaatcaaa
    98521 gcacagcctt ggggaacagt cctttatttt caatcaacag ctgtttagaa aatggtttaa
    98581 aaagatagga tggttgagat gataaaaaac ggtttatttt tatgtagtat tggttctttg
    98641 atcgtgttgt atagtttgaa tccacattcg caaaggtatg atttgtatag tatggtcaca
    98701 ggtctttttc taacaggatt aggaatctat ttcttcatga aggggaaaaa aagagaagaa
    98761 gcaaaggaga aaaataacta atggaaatca cgaaaaaatt aaatattcca gcatcgtttt
    98821 tttacgataa agttatggac tctgtccttt tcgatgtacg taaagcgacc ggcaaatcag
    98881 taactagaaa acagctgaaa aatttggagt acgtcaaaca attctcgcaa aataaccggg
    98941 ctcgcataaa aatcgaagaa gtcattgaaa accagtctta taagtttcgt acatcgacaa
    99001 cgaagaatga attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg acctgtgagg
    99061 ttcattacac agaaaaaatg gagtcttatg gcatgcttca aaagttgaat gatatgcttt
    99121 taggaacaat cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta gatatgatcg
    99181 aagcaacgta ttaaatcaat gaaacatggt actatttagt tattgaaaaa tgaagtaagc
    99241 tagaaggaat ttttcatagg aagatagatc tttcctgcga atcctcacta gcttttttgt
    99301 aggaaggggc agcaaaatga caaacaaagt aagagagcta ggtgtatctt cgattgagga
    99361 aatgtttgat ttagccactt atgcatttaa tggggcagat acacctgaaa ggcgagaacg
    99421 ttttcgtaca cttgcggaga attcttggaa ttacggattt tttgatgagg aggatcggct
    99481 aacaagtcaa gtcatggcga ccccttttcc agtgaatttt tacggacaag agtacctaat
    99541 ggccggcatt ggctatgttg cttcttatcc agaagcaagg ggacaaggcg ggatcaatca
    99601 gatcatggaa aagatccttg aagattgccg tgatcgtaaa gtaagcttat cttatctggc
    99661 gccattctcc tatcccttct atcggcgcta tggctatgaa caatcatttg ataaaatcag
    99721 ttatcaacta aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa aaataaagcg
    99781 tatggatttt gaagatgcaa aagctgcaat cagaagaatt tatgaacaga tgccggaaaa
    99841 ccaacgagct ggtctgaagc gcgaagactg gtggtatacg tataaattca agcaaaaagg
    99901 caattaccag tttgctttgc attttgatga taatgaggaa gcaacgggat atatcgttta
    99961 tcagcttgca acgtcttcct ttattattgc ggagtgggga tttttggatc atgatgcttt
   100021 ttgcagcctt gttcgcttcg taagttctca caatggtgct tttgaaacat ttgaatattc
   100081 atgcggctac ggcggtaatg atcaaaatta tctattagaa aatccatttg cttctgtaat
   100141 gatcacacct tatatgatgg cgaggatcgt agatatttct ttgtttttgg aaaattatcc
   100201 atttaaaaag agacaagctg catttgccct acaaatagaa gaagatcatt atgcgaaatg
   100261 gaatgaagga atcgtggaag taacaatcga aaatggaaaa aatcatctct caaaagtaga
   100321 acaaacaaaa ctcccaatcg ttcgaggaag tatccaacat atcacgcagc tattattagg
   100381 gtatcgtaaa gtggaagagt tggtattcca agaaagaata caggtgacaa aagaatatga
   100441 agaaacgtta agctcaatct tgccacagca acagccaatt cttaatgact atttttgata
   100501 aaaaacgggt gatgacaata tttttgtcac cacccttttg tttttcattt ggcatttgaa
   100561 ggaaaatgtt aaagtaaatc ctcttttttc gtaaatttga attttttgtt ttcttttgtt
   100621 ttagggtttt cttttttgtg gtcttggatt gcttctttcg taatcttttc ccgatttttt
   100681 ttagatgaat ctaagcgttc tgcgtatact ttttcttttg gcttatcatt cattggaaag
   100741 gacctcccta cattatttca ttctacctct agtttatcat ctgttaagga ctagtggaaa
   100801 cgttttgcct aacttataag ctgatttttt tagcagataa gcttgcaagt aaaaaacaaa
   100861 caagctacaa ttgatttatt gaaattgttc ctgtagctca gtaggataga gcaatcgcct
   100921 cctaagcgat aggtcgctgg ttcaattcca gtcaggaacg tataaataac ccctgtatcc
   100981 tttgcggtac aggggtttgt tttgtatttg tgccaaaaat gtgccaaact agttatcatt
   101041 tttatataaa ctatcgaaca cagtattgat tttttcttta tcctgatctt caagctctcg
   101101 aacaatatga agataagtag acattgtagt ttctaaactg ctatgtccta aacgtttcga
   101161 tacgctaagt atatttactc cttgataaag taaaatagat gcatgtgtat gccttagtcc
   101221 atgcaaagta aattgctttt caatccctaa ttcttttaat tttttgcgca aatatttgct
   101281 gacggcgttt gatgaaacta atccattttt taagttgaaa aaaacaaaat tatgagggtt
   101341 tttgatttca aaattctcga ataattcttt ttgattgatt ttaaactttt ttaagagatc
   101401 aatcgtatgt ttatcaatgg aaattttcct atttgaagtt tcgttttttg tttttcccca
   101461 ttcatttaat ttataattcc atgttttatt aatagtgatg atttgatctt cgaaatcgat
   101521 atcttcccat gttaatccta gaagttcagc aaatcgcatt ccagtggcac cagcaactaa
   101581 cacgagcata ggagaagagt actgcgcaga aaggttttct tctacgagtt tcattaaaga
   101641 tttaaattga tcataatcta aatatttatc ttcttttggc ttcaatgaat cttttccttt
   101701 gattactgct tttctagttg gatcaaacgg aattaatcct tcttctacgg catctttcaa
   101761 cgacgctcta atatggttat taaacttaat aacggtagat tttacatggt ctttagcata
   101821 cttatttaaa aagcgttgat atcccactct atctaaatca gaaatcaata ccgctggcat
   101881 atatttcttt atgttcatta acgtatcttc gtattttcta taagtgatag gagatactgt
   101941 tccttcttta taaagttgca tccaatcctc aaagtaatct gataataata gatttttgcg
   102001 atccccttta aggcctttgt tcaagttata ttctaattcg ttggcggcat cttttgcttc
   102061 tccttttgtc ttaaatcctg attttcttat cttcgtgtat tttccgtcat cttttttgta
   102121 tgaaatttcg tattgccaac tatttccacg tttgactaat cttgccataa ttgaatcagc
   102181 tctctttctc tgatacaata ggaactataa agaagcccat tgtttgggtt ttattatttt
   102241 ttgcacatcc tttttgcttg ccggcgcaag gatgtgctat ttgtcgtttt aatggtataa
   102301 ttggatgtgt attagatcat ccaatcagag gtgtttatta tgattaaagt gatttttaaa
   102361 agaataactg aacttccaga gtttagatta tttgttgatt attggtatgt ctggtttttt
   102421 attgttgcat tgctgatagg tctagatgtc atattagaaa gaaaacttaa gtaagagttg
   102481 agttaataaa ttgaattcct aaatgagata ttttgtagcc catattttgt ttgccgacat
   102541 cacctaatac tatggagcta atcgtttttt ctaacagacc aaaatcagat aatctattta
   102601 agctaccatc tattagctct tgtgaatagc cagacatgct gatatttcca gtgaaatttt
   102661 cgtttccacg actcattaag catgctaaca attgaatctc aatcgatgtt aattgattca
   102721 gtacaagaag ataatactcc tcgttatccc atgaagcgtt gttaattcga agtaaacaat
   102781 tcttataaag atttttatac atatctattt tattttgtgc ctttgatttt tctacctcgt
   102841 catttatccc ttcgaatatt ccaatgagtt ggtccttgtt ctctaagttg aaaacatctt
   102901 ctggaatttg atcgcgaatg ttttctaatg attcgtttaa atttttgtag aatttctcga
   102961 tgcgtttgaa acgcttctcg tttttagacc caaaataaag tgactgcaca acgccaccaa
   103021 tataaggaat tgctccaata ccgccttcaa ctattaattc aagatattct tttttggtta
   103081 gtttttcatt gctattatcc atagttacct cctattttgt catttccttt acaagcattg
   103141 tgactcctga tggcactctt ttgtataggt tttattattg tgcacaatct aactcttagt
   103201 ggggcgtggt tcatttattt tttacttact ttactattaa tcatatccca ttgagcatag
   103261 gatttttcta tgttcgatcc tcttgaacta atttctcctg taaaagttac tttatcgccc
   103321 tttgtaaagg tatcagcatt gtttaagtcc ttcgcaaaaa ttacatatga aactttaggt
   103381 gttgcagaaa tatcattcca acttttatca tcgtactctt tgtctgctat aatggctatt
   103441 ctagtagtca ttgattcgat aacagttcct tcaaaagtat atgttttacc actaatatat
   103501 ttagtaaaag ctgtattttg ctcagtagct ggagtaatag aataataact atctaagaaa
   103561 gcaccccagt catgattatt tgtctctaaa aattcttcaa attgagttag gcttttattg
   103621 ctgctgttag atgtagtagt ttgagtatca gatgatgagc tttcctgttt gttattgtca
   103681 tttgcctcgt ttgatgagga aagcgagaaa cctacggtag taaaaataat cccgataata
   103741 aggagaatca taggtgtttt cttatttgtt tttttgataa atcttacaat taacatgatt
   103801 aaacctataa taaataaaac aataccagta aaaccaaata taccgtccat taatagatcc
   103861 tctttctctt ttgatattga atattataaa ccaagcaatt cttttttctt aaaatcgaat
   103921 tcttcttgat taataatccc ttcatctaac aactctttat actttttaat ttcatctgca
   103981 cttgaaattt gtgtttcttt ggaattatta tctaatatag attgttgctt ctctatatat
   104041 ttttttaaat tttccatttc attccaatat ttttttgtaa acataacggt gttttcatct
   104101 ttcgtggccg ctaatatacc tccgcgactt tcgtttccgc ctagcaaggt aaattgtata
   104161 taaccattac tcatacctgg tttttttaat tgtacggctg atatattttt aaaggggata
   104221 gttttttctc ctttaatacc ttgattaact aaatttataa atccttttct aataattgaa
   104281 atagaatcat cagttacttt aatcaaggtt tttcctggag attttattaa aatctctttt
   104341 tccatttttt cttcctcatt tctgtgatat gatttttttt gtagaagctc atatatgagg
   104401 aaagagcccg tgttgcagca cgggcttttt taatgttttg ctgaaatcgg tttttttaat
   104461 aattcttgat gtattcatac atattgcctt gcgtaagaat attttttctt aaaatagcat
   104521 tggcagacag cataacaagt gtatctgagc ttattctgaa tagaataata ctccataaat
   104581 ttttcgagat taaactgaga ttcatcagtc agttcgttct caatatagat atttaataaa
   104641 attagaatag ctattttatc tgcttctgtt tcaaattttg agtgaaaagt tgtagaagta
   104701 tcgtacaaaa ctgaaaattc aaaatgagag gcgctgaaat gtgcaagttc atgagataaa
   104761 tgaaaggctt ctgcagtttc accgtataga ttttcattca aaaaaatgat tctgggtttt
   104821 ggatagtaga aaccaggctc tttcatctcc atataaacta ctttcaaatt gtattcgctc
   104881 aacatttctt tcaatttcaa atacatacaa accatcactc caactattca ttttcctcta
   104941 aagctttagc aattgcaatc gctttacgca ttgtctcctt agatatttct tttccatcaa
   105001 aagaaaaaac agtatcgtct tctgataaat ccacatgttt aggggtttct ctttcttctc
   105061 tacctagaag gtagtctaca gagacatcga aatagtcagc aatccgactt aattcatctg
   105121 aattaggagt attatttttc cactttgcta gataaccgtt agaatatcca aaattaattt
   105181 ctaactgccg tatagatatc tttttcctct tcgccaagtc ttttattatt tcataggtat
   105241 tcatcgataa aacaaccttt ctaaatgttt acaaaaaaag tttagaaaaa tacgcagaaa
   105301 taatttgact aattctgagt aatgagctat actatatctt gtaaacagat ttaacaacta
   105361 aaaagacaac aaaaaacact attgatatgt aaatgcagac cgccaagaaa gctttaaaat
   105421 caatgttttt atgtcttatt taattatgct cttagtatag agtattactc agatactgtc
   105481 aattgaattt agaaaaaagt tgttaaattt gtttacgaat ataaaagaaa ggaatgagaa
   105541 atatgaacac accgcaaatt tttaatttcg aacaaaacga agttcggaca attttagtaa
   105601 atgatgaacc atattttgta ggcaaagacg ttgcgagtgt tttgggttat tcaaacacta
   105661 aagatgcttt gtcgcggcat gtagatttgg aagataagat ggggtcgcga atcacgacct
   105721 caggtcaatc aagagagatg acaatcatca acgaatctgg tttgtacagt ttaatcttaa
   105781 aatcaaaact tccctctgcc aaaaaattta aacgttgggt aacaagtgaa gtgttgccag
   105841 caattagaaa acatggaggt tatctaactc cagaaaaagt agaagaagct ttgcttaatc
   105901 cagatacaat cattcaatta gcaactcaac taaaagaaga aagaactgga agattaatcg
   105961 cagaacaaaa gattgcagag tacgaaccta aaatctccta tttagatagc atattatctt
   106021 ctacagattc agtaacaatt agtcagattg cagcagatta tgggatgtct ccacaacaga
   106081 tgaataaatt acttcataaa ctaggtattc agaaaaaagt cggtaaccaa tggttattgt
   106141 gcaaaaaaca catgaaccaa ggatacacaa aatctcatac aactgagatc ccgaaagccg
   106201 atggtggcac taaaattgta atgaatacca aatggacaca gaaagggcgt ctatttatct
   106261 acgaattact aaaaaaagaa ggatattacc ctcaaatgga tttagaggaa attggttaga
   106321 aaggagtttt agtatgactg acattgcaga aatcactcaa cgagatagag aaaaaatcaa
   106381 agaatatgtc gaaagttcaa agttcttaac ttacaccatg cttgctgaaa gatttggaat
   106441 tagcaaaagc tacttatctt taattttaaa cggtaaaaag acttctgcag aagcaaacag
   106501 aattatagat tcgattatca ctatgtacga attgtaaagg gaggaacagc taatgcaata
   106561 tctagaagca aaaatcccaa ttccagaagg ctatgtaatt atctcccaag tggattatga
   106621 ggagttaaaa aaagctgatg atactggtag atggatgacg ttgccagaag tgctagaacg
   106681 gattaacaga aaatatgatt ggtttacttc tagagtttta aagaacccaa gatatagaaa
   106741 tattatcgat atcgagaaaa acaaaaatgg atttgtctat tatccagttg aaggaagaga
   106801 cacatatcta tttttaagaa gtaaaacact tgaattttta gaaacaaatt tttcggaaat
   106861 cttaaggagg caagcggatg ggaaaattta atagagcgct agtgttcagc gcaccgctaa
   106921 tcatctacgc tttaggactt tggggaagca ggcaagcgtt gataggaacg attgtttaca
   106981 tggtttggat ttttatgggg cttgatgaag ctgaagctga gtacagagcg aaaaagccaa
   107041 ccgagggggc tgactaaaaa tgaatagaaa agagaaacta gaatgtatat tactattact
   107101 cagtttaatt ctttcactaa tttctctatt gggaagtttg tatttttgat atcaacaatt
   107161 ttgtggattt gggttctatt tagagtaaat atcatttttg tttggtgatt aaggagttta
   107221 ttaaaattaa ttttccctac ttcaaatgca aggtaacaag atatagcact tttaggagga
   107281 atatttagcg gtacagcttc agagtatatt ctattatcat cgactgatcc tcctttagca
   107341 attaatttct taaatggagt agctgaccat ctgcggttta agttagcgga tgaaagttct
   107401 aaattaacta acatagctgg ttctgttgaa tagttagaaa tgattacttt tgtataaatc
   107461 atatctgcag cgaagtaaga agcattaagt tcaacttcta gttgagggcg cattcctttt
   107521 atttttatcc ctgttaaaaa cgtactcaat ataaaactta ttattgacat ccattgaaaa
   107581 attgttaagt gaaaaaaatt caaaataaac accaccagtt tttaactaaa ttataccaaa
   107641 aaggagagaa gaaataatgc aagaattagt aattttgaaa aataaagaag ctgtgactac
   107701 gagcttacaa gtggcagaaa gcttcgagaa aaaacatcaa catgttttaa gagatattga
   107761 tgcactaaaa aaagatgtgt ccaattttgg acagatgttt gtagaaggta atgaaccaga
   107821 ttcatatggc agaaatcgac gagttttctt cattagtaga gatggatttt tcttgctggc
   107881 tatggggttt acaggaaaga aagctatcta cttcaaacaa aaatacattg aagcattcaa
   107941 cgaaatggaa gatgttattc gcaagaatac tgttcctcaa acaattgaag atatgatgat
   108001 ctatcaatta gaagaaatga aagatgttaa aaaagatgtt tccatgctta aagatactat
   108061 gcgaattagc ggacaacaag agtttgaaat taagcaaaaa ggaaatatga aagttatggg
   108121 agttctaggg ggaaaagaaa gccgagctta tgaagaaatc agcaaaaaag tattctcaaa
   108181 attttggtct gaatttaaac gtaccttttc aatcccaaga tatggcgagt tacctcgtaa
   108241 gagattcgat gatgctgttt catttattga aatgtggttg ccagaaactg cgatccgtat
   108301 ggaaattgat caactgaaca gacaacagag actttttggt gatgacaatg aatagagctg
   108361 aagcgctaag aatagggacg gcaattgcta attgctggtg gaaatactat aaaccaatca
   108421 tcctaagcca acaacatatt gacaagcaaa aagcatggca acaaataaaa aagtgactcc
   108481 gccggcaagc aaagagtcac aaagaaaaca catcataagg agattttagc atatggaaaa
   108541 agaactttcc actctagatc aatatttgat tgatcctgat tggggcaagc cgaaaattga
   108601 ggaaacaagt ggtcgaaaaa tcagacgaaa tcttttgacg aatgaagaac tagcttggga
   108661 tcaagatgat ttaggcaacc atgtaactat ttgggatcat gtttatctta tccatctatc
   108721 gaagcattcg aataaacctg aatatattta cgtcatcgaa gatggcttga ttgatgcgct
   108781 agaagagtac gacagagata acttgattga tatctcttat tacggaccag gtaagaaata
   108841 cattgctgaa atggaggcag aatttgatga gtgaaatcaa agggacaacg aactttgaaa
   108901 aactttttag tcgtaagtta aataaaattc tcaagaaaaa aggaaatttt gattatttat
   108961 cttgggctca cgcgtgggag attatgaaaa agaatgatcc acaggcaacg gtaactatta
   109021 atgagtacaa acactacagg gttgtttctg gaactcatca agactttctt gttgaggaat
   109081 ataaaccttt tcttatggat gaaactggga cttatgtatc tgtctcagta acggttaaag
   109141 gacacacgga aaccgaatta tttcctgttt tagattatcg aaaccaacca gttgttaaac
   109201 caaatgcaat gcaaatcaat aactcattga agcgatgctt tgtgaaagca ttggctctac
   109261 acggactggg attatatgta tttcaagggg aagatattcc aacaccacct agaatcgata
   109321 caaagaaatt aaacatgcta gagacgattc tagaagcttt caatgagcag atgggtaaag
   109381 atatgaccaa aaccttaatc gaatatgtta atgagcagac agataaatta gggctcttag
   109441 ctgataacgt tgaaactatt gaacagttaa gctatgagca atgtgccttg atggagcgag
   109501 caatagcagc taagagaaaa gaattagata agaagtgata tgagtgttta aaccattaat
   109561 cgattcatat tcagcggttc tgaaaaagtt caaaggaaaa gacataagcg caaccatcaa
   109621 tgaggaagtg aacattgatc gactaaagac gatgtatgac ggctacgatg gtgatcgagt
   109681 cattgaaatt cgttttattg atcctagacg tttcaccgta cagcaacgaa acttcatcta
   109741 tgcgctgata ggcgatattt ttatcgatac aggcatgcca acggacttct ggaaggaatt
   109801 cttctacttt cgttttgaag gtgtcacagg gcgcaaaata agcctgaaag acgaatcgaa
   109861 tacaactgtg agtgatgcca atgtcttagc aaatatcatc ttagatttca tctttgaaca
   109921 tcatattcct ttcaaagaag gctatgagat tttacctgcg aatcaagaat attacttcta
   109981 caaatgcatc acaaaaagag tctgctgcat ctgtggcaaa acaggagctg acatcgatca
   110041 ctttgacaaa gcgctaggaa gacgaaagcg caaagaagtt gatcatgcag agtacacatt
   110101 tgcagcactc tgcagaatcc atcacacaga gaagcacaaa ataggtgtga ttaatttcaa
   110161 aaataaatat caaatcaaag ggatcaaatt aaaccagaaa acaatcaaaa agttaaatat
   110221 tggaggggaa aaatgacaga acatcgaagt tattatgcga ttataccagc caacgtaagg
   110281 tacgacaaaa gacttaaacc aaatactaag ttgttatacg gagagataac ggccttgtgt
   110341 aatgaaagag gcttttgttg ggcaggcaat gagtactttg cagatttata tggtgtgaat
   110401 aaagagacca tatcgcgatg ggtaagtgat ttgattaagt ttggatactt gaatcgggaa
   110461 atcatttaca aagagggtac caatcaaata atcaataggt acctacgaat taatcaatac
   110521 cctattgacg aaaaacgcaa tacccctatt gacgaaaaag tcaaagataa taatacatct
   110581 attaataata catttaataa tacaaaagaa tatataagag agttaccgcc ttcgaaaaaa
   110641 tcgaaggcta agcccgtccg tcataaatac ggagagtata aaaatgttct tttgtcagat
   110701 gagcagatgg agaaactcaa aacagaattc cctaatgatt accaagagcg aatcgaacga
   110761 ctgtcagagt attgtgaatc atctggtaag acttataaaa actatttggc aactattcga
   110821 agttgggcaa ggaaagaaaa aagtgaacct aagaacgcaa gcagtggata caagcgcaca
   110881 ggaagacgag agaagcttcc tgaatgggca atcgaccaag aagcctatct caagaaaaaa
   110941 gcgctagaac gagctaatag acaatcaaaa gcaccattct aagaggtgga aaaatgaaga
   111001 tcgattatct agaactaatt aatgaaatag cgaattataa aaagggcgag gaattagacg
   111061 tcctgagaga cgtatatgat caactcgaag aagctggaat tgaaggaatt aagaatgatc
   111121 gttcgagttg gagtaaactc agatactatt tcgcactcta tatcgatgga acgcaattaa
   111181 gaaatttagc atataccaaa ttactattca ttgattgtgt taaaggcttg caaaaacatc
   111241 ttaatgaact tgagcaggtg taaacaagat ggacctaaag acatttacag cacagatcga
   111301 attaatgcat caagaagctt taagacaaag tgtgtcgtac gaagacaagt ggctcaacac
   111361 atttcatggc ggacgtgaga gcgcacttga tcaagtactc aaattactga aaggagaatg
   111421 tcgggatgga taagaaagcg gcaatgcagc gaattatcga attgacttat tcagaagatt
   111481 ggcaggaaga taaaaaaata gttgcagaag tccaaaagct cggtaaatca atgtggactg
   111541 aaaagcccaa acggagaacg ccgagaaaga ttgcaatctg gcatggtgat cgaattctag
   111601 taacgggtac cgctaaacag ttatctgaaa ttactggtct gagcaaaaat attatttggg
   111661 atagagctag gagcttatgg attgattcaa aaggaagaca gtttaggtat gtggaggaga
   111721 aataatggac gaactaatcg cgaaagtaga gcagtgggca aaagataagg gattggatca
   111781 agcggatttc agcaagcaaa tgttaaaaac ggtcgaagaa atcggggaag ttgccgcttc
   111841 tctagctaga aaagatgaac atggtttaag agacggaatt ggcgatgtag tagtaacctt
   111901 gatcatcttg gcaatgcaaa atgatatgga tttatacgag tgcttggatt ttgcttatga
   111961 cgaaatcaaa ggtcggactg gaaagatggt caatggtgtt ttcgttaagt ccagtgacct
   112021 gaaataattc cattaatgga agaaataact gaataggagg atagacatga ttgatgcaaa
   112081 acaagtgcaa aaacagaaag atggaatgtt gatgtttgaa gcatatgttc taccatttct
   112141 taatcaattt gaagttttgg aatgctctgc cagcggagaa gaattggagt atgttgtaat
   112201 tcgtgaaacc aaagaaaacg ttcagaagct aaacgaattt ctttgcacta ttaactgttg
   112261 ggatatgata gctccaggat ttttatgtcc agctatggga gaatttttgg aatattgtcg
   112321 tcttgaagat gcaggcactt tggatttagc ttatctggta tacaactacc taaacatcaa
   112381 cacagatcat ttgtggtttg gcactgccga aagaaaatgg gtagtccgct aaccaccaat
   112441 atcaatgaaa aaaggcggtt tcctgaaagt ggaattgccg ccattgctgt aaggttcttt
   112501 acgcgactca aggtcacaga aatataaatg caaatctctt ttgaagtgat ccaaagctct
   112561 cggaaaatgg aagttttttg tttcaaagaa taaatgagat ttgcgtcaag aacctgagag
   112621 ttatttccaa aaaagcttcc aaagaaccga atgagctaag gacatcatgt gtaaacacat
   112681 aatatagctc tcctttcacg gctaatcata acatgaaaaa tattcgatgt cagtaggaaa
   112741 aaaggtgaat tatgaaaaga atattaaatt atcccggcag taacagattc tgcaattaaa
   112801 ctccgaagct gatgtatcag tatcaactgt agaatattat gagcgtggag aaagtcaagc
   112861 agtcacaaat gtaaaaatcg ataacgtcaa cggtcttatc tgtttagatg aaggcgatag
   112921 tatgaatgaa attgctgcag accatgaatt atcagaaact gctttaattg atcaaatcga
   112981 attagctaga caatactttg ttccatatgc agatacctgg tccaagcata agaatgacat
   113041 catttttcca gaggaacaat gagagcaact gatccagtaa ttatccttga ggaagccaaa
   113101 tttatttgga ctcacgaaga gatagagcaa gcacgcttgc tcttttctca aggagttaag
   113161 ccgagcaaag tagctgaaat aatgggtcaa aagattcttg atgtcggatt gcttttgctc
   113221 catctagcag aaaaaatttg atttgaggtg aaaatgatga ttcaacttgc aggcatccaa
   113281 acagggaaaa tttatttttc tggagaaagc aaaagcgaag ccaaccaatg gctattgaaa
   113341 acttacacga acaataagaa gctaaggaaa aaatatcctg atttgttgct aaaggatgat
   113401 cagattatgc cggaaccgat gattttgacc cgtaaggagg gaagttgaaa ctaaaaaacc
   113461 agccgtgtgg gctggagttg ctatttcttt ttaataatga gtttttctaa atttaattca
   113521 ttctcacgag tttgcgtcca ataatcagaa taatatttta attgtttaag agtaggaatg
   113581 tcgtcttcgg taaaattaga aaataattct ggagaattat ctttttttaa ttgttctaat
   113641 cctgattctt taaaacgttg ttcaacgagt tttactaaat tgttgatatt catgataaat
   113701 caccttttta aatttaattt cgatttgatt aatcgataca agtattatat caaataaaaa
   113761 agctgattta gggggtctta caaaaacact aaaatttttt caaaaagagg aggaacggtg
   113821 gtgaaagtca gagaacaatt caagatagtg agcgacgaag gtaatgttta caaaataaag
   113881 atcgttaata ttaatgaata tcgagaagca gatcacaaat atttggttga cttatacatg
   113941 aacgataaat atttgggaga aggattttgt ggagattcac tttttgatga tgagcgaatt
   114001 ttgaaggagg aaacagaatg atccacgaac taaaaatact accagaatat tttgaagcag
   114061 tgacgagcgg acgcaaacaa ttcgagatcc gcaagaatga tcgtgacttc aaagttggtg
   114121 atcagttaat actacgtgaa tataaaaaat acattaaaca tgaattacct ggactaacga
   114181 gaccaacatt tagttacact ggtgactcat acaaagcaga gatcacttat attactgatt
   114241 acatgcagaa gtccggatat gtagtgttgg ggattaaagg ggtgaattaa atgaaagtat
   114301 acatcttagc tatcacagag ggcacatgga tgttcccagt cggttccgga aaaatctata
   114361 aatcaaaaac agctgcttat aaagcatttg aaaaatacaa aaaagaaaac ggtggcggca
   114421 ctaacgcaaa gattctagtg gctgataact ggcatgaaga aggtgaacgt aattgattta
   114481 cctgttcatt ttcgtggtac taactatttt tatgctagta atcttattca aaaaaagatg
   114541 agtaaagacg aacgaaaaat tctggccaac ttttatttag tattaaaaat cgaacaaaat
   114601 gtgttttcgg aaaggagaat tgtatgggaa aaacaaaatc aaagattaag aagaaaaaac
   114661 gacgtttgga acaaaaggca atccagaatg gaacggctaa gaaaaaataa aaaagctacc
   114721 cctttcgaga tagcagcgca atactattct accataaggg gtggcgtttg tgagatttca
   114781 ttggttaaaa gattaccaag aactagatga gcagattctt tacttaaagt ggaatcttaa
   114841 taaaagtaag cttgaattga atcgatgggt ctgtggtgat ttagcaaacg tccgcatcga
   114901 aaagaattcg agaacttctt ctttagaaga gaatatccaa aagatagaaa atgaattgga
   114961 attgttgatc gagcaaaaag aagaaatgtt attgctgata gatagctttt ctggtatcga
   115021 taatcaaatt gttaagatga aatatgttga tcaaatgagc ttagaagata ttgctgaatc
   115081 tgttgggtat agctcatcgt atgtcagaca gcgacatgca gaaattagaa agacattgaa
   115141 ctttttagat gagtatgaac acagacaagc tgaccgattg aaaaaagaaa acgaaattga
   115201 tttttacaat agcgagaagt acaaagaaca actctcttta ttctaaaatt acactgttca
   115261 ctaaatgttc gtaaacattg ttagcagcat gatattctat tagtgtcaaa aaaatatgaa
   115321 agagccaaga tatcccaacc gttttattaa ttggtatctg tggctctttt attcttttaa
   115381 ttagtagttg gcataaagtt atttccattt ataaattgat cagctaaatc agcatcataa
   115441 tcagccgcga taaattgcgc tgctgattta ataaatgcat ccatatcatg aacatcttta
   115501 ttagtatgtc tacgaacata gtgcgtttca tcattaccaa tccaagtagc agctttggct
   115561 aatctttgga gtttaggaaa atcattcaaa taattttcaa ttgttttacc taataacatt
   115621 ttttttattt tatcttcatc ctctgaattt ttagatattg cataatcttt aattagaaat
   115681 tctagagctt ttctataacc aactccagca atttgttcca attgctcaga tttagcttgt
   115741 gtggcttgag tataaatatc aacaaatgta ggagaaactt tttcaatatt ttctggtaaa
   115801 tctattttaa tcggcggacg gtaataatct ttaattaaaa tattttcaga atatccttca
   115861 tcttcattaa acgtatttat aaattgtaaa ctataaaatt tgttgcactc atcatttata
   115921 cactgtacta atacgccaaa gattctatca ttatcgaacg cactatatga tgaaacccca
   115981 aaatacacat acggagtcat tatatttccg caatgcggac aactatttgg caaataaatt
   116041 gtctctgtat tttgacgatc acgatactta atattaactt cttgaagcat taaaacacct
   116101 cgcatctttt ttataaatca taccacaaaa tatctgatag gagagtaaac aaatgaatga
   116161 ttttcatgaa gcggtactta gtattgaagt agaatcaagt ttggctaaag tttataagaa
   116221 agcaattgaa gcagagaata gtccgtacag agaaaattgg aatggtaatc atgcacatgt
   116281 gcaagtggag aatgatgatt acagaactgg aatgaatacg ttagttattt ctttattgtc
   116341 acacactcta cccaatctgc aggaaacgat tgaatggtat gagcgaatgg gcgcaaaagt
   116401 aatccgtaca aattacaaag gagagaatta aaatggcaat gatcaaaatt agaacatcca
   116461 ttagtggaac agagtattgg gattcagaaa agaaaaggac agtagttgtt ccgaaagatc
   116521 aagaaccaaa ttttgaagta aaagaagaaa aaggaatttt gagtgatggc aaatcatttg
   116581 ttgctgtcaa tggcgaactc atagctcata gcagtgaaat tcttgatagc gatggaaaca
   116641 ctgctgctaa ttttgatgga gacgaagcta ctaatgatca gtccactgaa gatacggatg
   116701 aactggacaa tatgactgca aaagaattgc gtgcatatgc taagaaacat ggtattgata
   116761 ttcctggtgc tatccgtgca aaaggcgata tcataaatct tattcgtgaa gtagaatgaa
   116821 atactgtcag tttgacggat gcacgaacaa aatagcaaag ggtatctact gtactgaaca
   116881 caagagatca agcagatcac gcaagaagaa gcagcaagca aagtctgttt atcatcatga
   116941 gaacaaacca ttctatcgaa cgcaagcatg gaaagatatg cgtcaattta tttatgaaag
   117001 agaaggtggt cattgtcagc ggtgtggtca gttcatattt ggaaagagag cacacgtcca
   117061 tcacattgta ccaatcaaag acaacgaact gcttaagctt gatccaaaca atctcatgct
   117121 attatgttca aaatgtcatc caattgttga aaacgaaacg gaagacaaaa aagtttttcc
   117181 ttcgtatttc aattgaagcc cccctattca ttttcaaaat tttttcgcgt ggggagatag
   117241 ggtagcgggg agtcacgcgc atcgttaggt caaatttttc aaaaaacaaa ggggggtgta
   117301 tacaaaaatg acgactaaag cgcaacgtaa agcgattatt gatgaaaaag taagtgctga
   117361 aaaagctcgt atcttagaaa taatgaattt gtctgatttg tacaccatca ctcttgatcc
   117421 attaatcgaa tcatacttgg atatttttga aatttaccaa cacaaatatt tattgtggaa
   117481 ggaaaaagga tttccggaga cgcaaaaatt cacgaataaa tcaggagcta ctaatcaatc
   117541 aaaacatcca ttggcacaac aagtagaaac ttgggcagat aagaagatga aagctctaga
   117601 tttattagga ttaacaaaca aagcaaaaac tggtagacta attgctggag gatcgaccgc
   117661 aagaaaagat gaagaaatta cacgcccaga agtaaagcca gtagatgaac tagcagcgca
   117721 cagaaataaa tggcgtaaga aggcaggtgc tgaaaaatga ttgaacctgg tgtaaattat
   117781 gctgatttat ttgcaaaaga agtaagaaag aaacctaaga agtatcctaa gaccgttcgt
   117841 ttagcaatag atcgctggta tcagtggaaa aaaagaaaag atatttggtt tgatgtagat
   117901 cgcgcgaatg aaatgatgga ttgggtagaa tcatttattg ttcacacaaa aggagaaatg
   117961 gtgggaaaac cgtttctttt agaaccctgg gaaaaattta tttattcttg gatttatggt
   118021 tgggttaaag aaaatgaaaa aggccaaatt gtacgcgtta ctcgtgaggc ttatgtccag
   118081 attccaaaaa agaatggtaa aacattgatt gctgtcggtt cattaggata tgcaatgtac
   118141 ggagaagggg cgttatcagt tgattgctat gcttgcgcat ctgattttgc tcaagcgcaa
   118201 tatgctgcca aaccttttgc agttacaatc ttaaacaatc caatcctact tgaaggaact
   118261 aaaatattta gggggccaaa gggaactgtt tctagtatta cttatgacta tattcatgaa
   118321 gatatggctt actcaaataa atttattgtt caaaccaaga atattgataa tatcgaggga
   118381 tccaatccat attttgtatt gaacgatgaa ttgcataaac aggagaaaat ggaacagtat
   118441 gacaatttta agtctgctca gatatcttta ccacagccat tgatgtttaa tatctcaact
   118501 gctgggaaag gttcgtcgtc tgttggaatg cgtgtatatc gcgaagcaaa agaagtgttg
   118561 aaacgtgatg ataatgattc aaactttgtt ctaatctatg agccaaataa aaattatgat
   118621 tggacggata gaaaagtctg ggaaatgtgc aatcctaact ggggaatatc agtcgattta
   118681 tctgctttag aatcggcatt taaaacagcg caacggtcag ctcattccaa agctgaattt
   118741 ctaacgaaac acttggatgt atttgtgaac ggcgcggata atttctttga acaggatcaa
   118801 gtagagccat gtttagttac cacacaagaa cttggtgatt taagtggcga accatgttat
   118861 atcggtttag atttatcacg tacacgagat ttgacctgtg tatctttaaa cttcccaaca
   118921 tgggatgaag atggtaaagc ggttcttaaa gtaaagcaac tttattttat tccgaatgaa
   118981 gatttagagt ttcgagaaaa agaagataat gttccttaca gtgatttggc tgaacaaggt
   119041 ttcgttgaat tttgcgacgg aaaaatgatt gatcaagatc agattttgca gtacatagag
   119101 gactgcatga atttatatga tattcagcaa gtgaattatg atccagcgat gagtgacaaa
   119161 cttgttgaga aattagaaaa cttaggattg gaatgcgttg aagttgctca atatcctaaa
   119221 gtattgaatg ctccttttga cgatgtcgaa cggttgtttt atgagaaacg aattcaattt
   119281 gataatccat tattccttta ttgcactttg aacgttgtag caattactaa catcaatgga
   119341 caaaaagcgc caagtaaacg tcaatcaaag aaaaagattg atgggttcgt ggcgtttttg
   119401 tgtggtcata aggaaacgat gaatcaaatg acagatattg attcagatga gttagatgat
   119461 tacctaagtt ccatctatag ataaatagaa aggcggtgaa aaaaattgaa attacgtgat
   119521 cggttatcga atgcagtcta ttcttttatg gaaaaacgtg gatatatcga ggatattttt
   119581 ggacactaca cacgttatgg tcaaagatat gtgacggatt catctatcat ggaatcttct
   119641 gatgtttatg aattagttca agacatatct aatcaggtgg cattggccac accaattgtt
   119701 attggtcccg acggcaacga agtcaaagat catcacttac taaatatttt gaagagtccc
   119761 aatgattatt tgacaggatt cgaatttacc aaattggaaa caaatacttt attgatcaat
   119821 ggtgagacat tcccattaac ggatagggat cagcttcatt tggcgtacgg tgtaacaact
   119881 aaaatcaatg aacgacttca agaagaattt gaaatgaatg gtcaaaaaat acctggtcaa
   119941 atgattcgac acattaagaa catcggaact gattcattaa aaggtgctgg aataattgat
   120001 cttgcaagaa acactctaga aggcgttctg agcgctgaaa aagttttgac ggacaaatat
   120061 actaaaggcg gtttacttgc gttcatgctt aaactagacg cccacatcaa tccaaataat
   120121 agcgcccaaa caaaaattgt caaagctata ttggatcaac tggaaggaac gcaaaatgag
   120181 agtgatcatt ctgttaagat gattcctttg ggaaaaggat attccatcga gacattaaaa
   120241 agtcctgttg atgacgcggc aattttgaat tatttaggtg tttacaaaaa agacttagga
   120301 aaatttctag gaatcaatgt tgatacgtat caatcgctga tgaagacaga tattgaaaaa
   120361 gcgatgatgt atctgcacaa caaagcaatc aaaccaatat tgaaaaacaa gagcgaacat
   120421 tacaccgctc ttttttttat gcctaattct ggctatagag tggaatggaa aattaatatt
   120481 ttggattttg ttccttactc aacaaaaaca aatattggct acaacatcgt tcgtaccggg
   120541 attacaagtc cagataacgt ggcagaaatg cttggttttc ctaaacagaa tactccagaa
   120601 acacaagcta tctatatttc aaatgactta tctaggattg gccagaaaaa tgcaacagat
   120661 gattccttac caacgaatga tcaagacttg aaaggaggtg atggaaatga agaagaagga
   120721 aattcgcacg attgacatca ccaatctttc aacacgttcc gatgaagaaa ctcatacgag
   120781 gaccattagc ggctacgctg ctgtattcaa cagtcaaaca cttttatggg atgacttaag
   120841 cgaagtgatt gttcctggtg cattctcaaa ggcaattagt aattcggatg ttcgttgttt
   120901 gttcaatcac gattggtcca atgtgctagg acgtactaaa agcggaaccc ttcgccttga
   120961 agaagatgaa cgtggtttga aattcgaagt tgatttaccg gacacaacgg tagcaaggga
   121021 cttagttaaa tctatggaac gaggagacat caaccaatgt agttttggtt ttgtgccgac
   121081 tgaagaaacg tgggactaca attctgaacc aatgcttcga acaatccatg aagtggaatt
   121141 atacgaggtt tctattgtgc ctttgccagc atacgaagat acagaagctg cattaagaag
   121201 tcgtgatgaa ttagaaaaaa acgtcgaaga aagaaaaaaa ttaatcaaaa aaattaatca
   121261 agcgctagaa gcgtaggagg aaaaatacat ggataaagaa ttattgaaaa aaatgaaggc
   121321 acgtcgtgag caacgattga ctgaattacg tgaaaaagtt gaatcaggag aattacgcga
   121381 agcagattta gaagccgtga aagaagaaat tgacagtgtt attgatgaat tgaacggaat
   121441 taaagacgaa ttaggcgcag attctggaac tgatgaaaca gacgacaata cagatgatca
   121501 atcgaatagt acggattcag acgaaagccg ttctggtgaa gacaacgatc aagaagaaga
   121561 ttcagacagt gaagatagtt cagaaaatcg ttctggaatg attactcaac agcaacgaga
   121621 tggattactt ggatcaatta agaacggatt ggaggcacgt gcaaaaatga ccaagaaaca
   121681 aaaagatcaa caactacgaa aagcatttgc taattttgta gctggaaata tttctgaagc
   121741 agaagctcga gctttaggga ttgaagctgg caacggttca gttactgtcc cagaagtaat
   121801 tgcatctgaa gttattactt atgctcaaga agaaaattta cttcgtaaat acggaacagt
   121861 ggtgcgaaca tcaggagatg tcaaatatcc aattcttgtg aagaaagcag atgcgaatgt
   121921 aaacaagaaa gagcgttcaa ctgatattgc tgaaacagct attcagtttg atgaaatttt
   121981 gcttaatcct gccgaattcg atgctttggc aacagtaact aaaaaattat taaaaatgtc
   122041 tggtgttcca gttgaagata ttgttgtgga agaattgaaa aaagcttatg tgcgtaaaga
   122101 aatcaattat atgttcaatg gtgatgacgc tggaaatgaa aatcctggtg cattagccaa
   122161 aaaggctgta gcatttaaaa aacctttaga tctaactgct tcaggtgctg ggcaaaaatt
   122221 atatgatgca ttaatcgaat ttaaaaatac accagtgaca gaagtgatga aaaagggacg
   122281 ctttattatt aatcgagctg ctttgactgc cattgaaaaa atgaaaacag atgatggatt
   122341 tcctttgttg cggccattca cacaagcaga aggtggaatt ggttaccaat tagttggcta
   122401 tcctgtggat tggacagatg cagcagataa aaagggtgaa ccagacacgc cagttttata
   122461 ttttggcgat ttttctgcat ttaaaattca agaagttatt ggtgccttgg aaattcaaaa
   122521 acttgttgaa aaattctctg gtaaaaatca aattggattc caaatttaca acttgctaga
   122581 tggtcaattg gtttattctc catttgaacc ggcagtatat cgctacgaaa ttacaaaacc
   122641 agttggtggt taagatggaa gagcaaacta aagaattgtc tttagaggaa aaattcaaat
   122701 cacatattca ttttgaagag ggcatggatg attctttgct ctctttttat ttaaatatgg
   122761 caaaagatta tgtcaaaaca gcaactggtg gccaacaaga atatcttatt ttgatggttg
   122821 ccggcattgc ctatgaatat agagtttcag aggatgaact cgacaaagct atgaatgcca
   122881 tgacgccatt tatcgtgcaa ggagcgattc aaaatgccga agagacagac taataatctc
   122941 agatggaaag ccgaattgct agacatcaaa acaggaacag acggaaacga tcgtccaact
   123001 acaatttacg aatttaagcg tctaatattc tatgaagaac tcggtgtgac ttctcaagaa
   123061 aaatatttat cacagcaagc caagacagac gttgtcagac gaattaaagt cagatgggat
   123121 aaatccatca cagagaaatt aagtgcgctc aaaattgatt ctgtaacgta taacattact
   123181 cgcatttata cgaatcccga tgcaagagaa atggagttga gtttagctta tgtcgattag
   123241 ctttgatgaa ttgaaaacag cgctgaaatc aacaaagtta gcagtgttca gagacaaagc
   123301 cagattaggg acgatgtatc catacattgt gtactcaaat gtgagtaaca gcaaaaaaat
   123361 ggcatccggt aaagtctata aaaaattacc ttattaccaa gtctcttttt ttacacttgg
   123421 aacagaacag gatttagttg ttattgaaca agcgctgcaa aatgctggca ttccatattc
   123481 agattttaca ggtatacaag gtgatgagaa cgacgatacc gtaaccaact attacacata
   123541 tgtgaggtgt gtggaaaatg ccaagtaata agaatggttt ttctgaaata tcggattatt
   123601 taggaaacct ttcaaaagtt gatccgaaaa aattatcgtt ggaatcttta gaagaagcag
   123661 ctaactttta tctaaagcag ttgcttccta atattcctaa gtcgctctta aaaaagaaac
   123721 atatgcgcga tcaaataaaa gtagtagttg aaaacgatca agtgaaagtt caatttgaag
   123781 agacggcctt ttactggcgc tttgccgaaa atggcacaac aaatcaacgt gcccaacatt
   123841 ttgcaagtgg tacgtatgaa caaaacaaag aaaaaattga agaaattatg actagaaaaa
   123901 tacttgattt atgggaaggg tgattttaat tgggaaaaca agatactttt tattttgaag
   123961 gattggacga cattttaatt gcgatgatgg caacgccaga ttctgttgga actgcaccga
   124021 cttatagtga agttttacga ttgccaattg ccacaaaaat cggtgtcaaa gggaatggaa
   124081 cagcattaga aaaatgggca tccagcaaaa tgtttcgtcg tgtgtcgcga gaaacaaaac
   124141 atgaacttgc gttggatcac gtgggtattc cgatagaagt gatggatgaa atcaaaggat
   124201 tagttgctaa aagcggagta acgtttagca aaaataccgc acgagaattt ccgtacttcg
   124261 cctttggatt tattggaaat attgaaaatg gcggtaaaaa agcagtttgg tatcctaata
   124321 cacaactatc caatgtgatt gacgaagagt atgccactgc agaggaagaa acaaaaattg
   124381 acgatgtaac tgcaaatctg gtttccatcg gactgaaata caataatgtg atgtatgcaa
   124441 gttttgattc aaatagagac ggagcttctc tagaattatt taataaattc attgctcaac
   124501 ctgtatacga tgaagaacag tggaaaacat tggctaaagt tggaggtgcg ggttaatggc
   124561 tcggttatct gattatggaa ttcacgttga agacttaaaa aattctgctg ttgtaattat
   124621 tcaaggcgca gaattcccta tttcatttac tatgcaaacg atggaattta tagcagatgt
   124681 gtatggtgga gattattcgc aatttgaatc tgatatgaac gccatgctat ctaaaaaaga
   124741 aggacaaatt tcttctgcta acttatcgcc tagtgatcta aaaatcatgc gtgccttgat
   124801 ttatgccatg ctgcgtactg gtggtttaga ggaagatcca gaaactattt tcaaattttt
   124861 gggaatgagt ggagaggtgt tgtctgctta tagtacctgt atggaaattt ttgctagcca
   124921 gacatttcag gtggaagacc taaaaaaatc caagaagcca caagactttc aaaaagcgca
   124981 agcaaaaaga aagaaaaaca aaaagaatcg gaagagatag gaactccttg gagtttttat
   125041 atttacgttg ctctcactct attgaattgg agtgagagtt tctttttaaa gtctacacct
   125101 aacttgtggc tcaaatctta tttacagtgg ttacaacaaa acaccgattt tgaaccacct
   125161 caatctgtaa ctatggataa atcgccttgg tggtagaaag gagcgctaag ataaatggct
   125221 ggtaaagaat ctgatgtcgt tcttaatttt aaaacgaatg gcgaagtcag ttattcgaaa
   125281 acaatcaaag aaatcaacaa agaaatgaac ttagccgctg ccgagtacaa aaaccaagtg
   125341 tctgcgatgg acaaggatgc aactcaaaca gaaaaattgc gagcggctaa acaaaaatta
   125401 gaaaaacaat taggattggc tgaacaacga tctcaaatgc tgagagagga atatgaaaaa
   125461 tctgtcaaag aaaccggtga atattctgct gaatcagaaa aactttataa acaattactc
   125521 aattctgaaa ccggagaaaa taaacttcgt acagcattag aacagacgaa cgatgcactt
   125581 aaagaacaag gtgacgtttc tgttgataca gcaaaaaaac tccagaaaat cgaagaaaca
   125641 ggtgagaaag taaaaggcgt tggtgaaaaa atgtctgttg gagtaaccgc gcctattgta
   125701 gcggcaggag cagcaggact tgcagcattt ggtgaagttg acgaggcact tgataccatc
   125761 attacaaaaa ccggagcaac aggtgatcaa gctgatagac tttcacagtc tttcaaaaac
   125821 gttggttcaa atactcattt acctttacaa acggttgggg aagctattgg tgaggtaaat
   125881 acacaatttg gattcatgga taaaaaactg gaagattcaa ccaattatct cctacagtac
   125941 gctgaaatca atgatacaga tgtttcgcaa tcagcaatat ctgctcgaca agctattgat
   126001 gcttatggac tagaatatga tgatttgaat tctgtccttg atgtaacaac gaaaacatcg
   126061 cagaatactg gtcaatctgt agacgacttg atgcaaaaag caattgatgg cgcaccacaa
   126121 attaaacaat tagggttgag ctttggtgaa ggggtcactt tacttggaca atttgagcaa
   126181 tctggtgttg attcaagcgc agctttaagt agtttatcta aagcaacagt ggcttatgcg
   126241 aaagacggaa aatctttaag tcaaggacta gaagaactac aagacaaggt aaaaaatgca
   126301 ggttctgaaa cagaagccat caatgcagca gcagaagtat tcgggactaa aggcggtcct
   126361 agaatggccg atgcaattcg tagaggtact ttgaacctgg aagatttagc aaaaactgct
   126421 ggagaaagtg ggggagctgt aggagacaca ttcgatgcta ctctcgaccc aattgatcaa
   126481 gccgatcaag ctatgaacaa tgcaaaatta gcaatggctg acgtaggtga agcagtccaa
   126541 gtaagccttt tgcctttttt tgaaaaagca acttccttat tacaggaatt ctctaaatgg
   126601 tgggggtctc tagatcaaga tacaaagaat tggatcataa caattgctgg tatagcggca
   126661 gcagttggac cagtactaat cgttcttggt tcacttatgg gatctgttac aaaaatcgtt
   126721 ggggaagtta aaagttttat aggagtttgg caaggacttg ctggtttatt tggaatgtcg
   126781 ggaggctggt ttgcattagc agttatagct attggcgcat tagttactgg actaatttgg
   126841 gcatataata acgttaaatg gtttcatgat ggagtgaatt ctttcttcaa aggtgtttca
   126901 gatgtagctg ttgaaatatt taatttttta agtggttatt ttggtggatt tttcgatggt
   126961 gttatagcaa atttcaataa tttttataat gctggaaaac gaattttcag tggttttcta
   127021 gattttatca ctggagtatt tacagggaat tggtcaaaag catggcaagg tctagttgat
   127081 atttttggag gtattttcga tggtattgtt gctgtaggga aagctcctat taatgccatg
   127141 attggattga tcaatggatt tattggtgga ttgaataata taaagatacc aaaatgggtt
   127201 cctggtgtag gtggaaaatc attttctatt tcaaaactgc cttatttagc acaaggcggt
   127261 catctaatca atggacaagc gattgttggt gaagctggac ctgagttact gactgccaag
   127321 aatggtaaaa ccacagttac tccattatca gacgaagaaa aacgcaaagg gatcagtgga
   127381 agagtttctg gaggtagcat tgaacagcat atacacattg gcaaagtcga tgcaaacaat
   127441 ccatctgagt tagatcgtat gaatcgtaaa tttgctaaag caaacaaaca ggctattcgt
   127501 gatttgggag gtgttcctat ttgagtcgac aatttatgaa tccagatgaa ccaaatttta
   127561 tttggaaaaa tcggaatgca gtaattgata tgaattgcat cattgaggca gagcttccag
   127621 atattatgcc gaacaagcga tacgaaactt acacgattca gggccgaagc ggagaattga
   127681 cggagacgtt tgatgactat gaaccttttg atttggaaat agaagggatc actattccac
   127741 attcgaaact gagagaagtc aaacgatggc tcactggtaa aagccaatta atcacacata
   127801 atgatccaga caagtatcta gaggctatct gcagcatgga taaagaagtc cctttcgaaa
   127861 acgagtgggg cttcttttat acattcgatg ttacttttcg ttgtcagcca ctcaaaagaa
   127921 aaatcggtga agcacctaaa atttttcatt ctagtgttat ggatattttt gatcatggtg
   127981 acgaaattgc acatccctat ctggaaatag aatcgaacgg aggagatata acgatcgctg
   128041 tcggtgataa aagcctaaca atacttaata cgttagctgc aacaatcgtg gttgatactc
   128101 agcttggaaa agcaatacaa gaaggtttga atttatttac gcgtggtgat tggccagtat
   128161 tgcagcctga atggaatcag gtaaaaatat ccggaaaatt taaagaagtg cggttttgga
   128221 ataggagtgt gtatctgtga cgcaagaatt tatttatgct tataaaaaaa tgccagatga
   128281 tttgagcgtg aacggagcgt cgttagttga ttgggaagat ttgccagaaa tcaatcgtgt
   128341 gctgaatggc caatatcgtt tttatggtaa ctattcaaga agcggacagt atcgctctta
   128401 tttaaaaaaa ggaaatttta taaaagcgaa ggtaccagat ggatcatggc aatattttga
   128461 aatttataat gtcaaaaaga atttgacctc cgtttctgta acagctcggc atattggttt
   128521 tatggccaat aagaacttta ttgtcaagtc gtttacggat aacggcaatg ggtctcagat
   128581 catgaccaat cttaaaaaca gtttggcatt tgatcaaaaa tttaactatc tttcgaatgt
   128641 agggacaacg caccaattta cagcaagaca agtcgcgcca gttgaagcaa tcattggttc
   128701 aaataacggt aatcaaaatt tgactggcgt gactagcgcg gaacttgata tggataacta
   128761 tgatttgaaa ctggtaaaac agattggatc agataatggt tttcggattg attttggcat
   128821 caatttagaa gcaatcgaag aagaaattga tgaagaatca attgtcaata gtttgtattt
   128881 ggttggcggt gttcctgata acgattatga tgaagacaaa gaacctatcg aatatggtta
   128941 tttagaaatt gatggagtaa ccaacgagaa tcgacgtatt gccaaacgtg aaaacggaga
   129001 ttgtaaaaca gttgatgaat tgatcaaatg gggcaaaacc ttatttgaca atgatcgtat
   129061 ccatgagcca aaagctactc atacagtcag tatggtggcg ttggaacaca cactcgaata
   129121 cggtgacatg tatcaagagc tagcgtcttt acactttggt gatgtcgctc atgttagggc
   129181 gaaagagcta gatatcgaga taaaagagcg tatggtcgaa tacacttatt tcccaacgct
   129241 tgggaaatat aaggatattg ttttaggtaa tgatttatca ctgtacactt cggcagtaaa
   129301 tactcaagca caagaattga aaaagaaaat cgacaatcga acagaaacgt tagttcaaaa
   129361 tgtgttgaat gcgacggcat ggatcactgg taattctggt gggcatgtcg tttttcgtcc
   129421 agaaaaagct ccatcagaga ttcttatcat ggatactcca aatgtggcga atgcaaagcg
   129481 agtgtggcgt tggaatttaa atggcttagg ttattcagac aacggagtaa atggcccgtt
   129541 cggtattgct atcacatcaa aaggagaaat tgttgctgac ttcatcaaag taggaacaat
   129601 aaatgcagaa gtattcgaaa cttcctttaa tgcttatggt gatgtgttaa aacttgtaaa
   129661 aggcacgctt caaatttgga atgaaaacaa aaaaatcatg gagctaacca aaaaaggtat
   129721 ggaattctgg aatagaaaag aatccattgg aacgatcgga acaacagatt cagcaggaaa
   129781 tccatttcca aatgctgtaa cacctacacc tcttgaggaa aattctttgg taatacgtac
   129841 aaatggcgat ggaaaataca ttttaatttc tccaactgca gaaaaaggat ttgtgttatt
   129901 aggaaacgga aaagcatatt atttcggtga tttagatatt cagggaaaac ttactgttag
   129961 aggtaaagaa gtgataccag gacaaaatgg cggtccatct ggcggaggag aaacacctgg
   130021 tgggtatcct gatgaattaa aaacagatgc agaaaagaga gcttggagaa t
//
LOCUS       DANJMI010000002       104764 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000002 DANJMI010000000
VERSION     DANJMI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 104764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 104764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..104764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.002"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(59..187)
                     /locus_tag="PWU81_000152"
     CDS             complement(59..187)
                     /locus_tag="PWU81_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL1108891.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(322..4845)
                     /locus_tag="PWU81_000153"
     CDS             complement(322..4845)
                     /locus_tag="PWU81_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HDL1108892.1"
                     /translation="MSQWNTIDELRDIFIRKYTDSELSNELEKACSEEYELMRDYNGR
                     QILELLQNVDDAYGDIKSENDASNDEVKVQITYKNNILEVGNTGTSFSKDTIERLCLG
                     RASNKSSQNIGNKGTGFRSLLNDAEWVELYSGEFAIRFSEEFTKKLFEQYVSRDSDKF
                     SELIFEQKKNWKKEDYDLCFPIMNCPQPINKIDSGFDTLIRVKLKETNLSKGTSVSKQ
                     LQQPFYKSLLFLPNITKIVIETTDDEKISEKIVDGNDVLIEKSDVTGQSPSEEYFVFD
                     KESMIGDKVAKIALAVSKDSDYDYSKEKLYCYFPVRNFGTPIHALINAPFITNNSRDD
                     VPDDSEQINKKIFIEVLKFISEVAEKLSKPEYEDIALKMVTPFKENKLWDLDVFDLMN
                     EFLGILSNAQILPTVNWQYISIKDNPKLFLQEFPEELIGETFSTLLQLLETNENDQLV
                     QELANYNEYTGLSFVVDELSDKINILSSSWENRIRTKVFLWWSEQYKSSPIVPKLLKD
                     NSDNWLMKSDKVFLPTDTGVTVLPKELSSWVSLSILKQTYVDELIYQIKTSSSWKNKW
                     EETANAYKAERTGDKRILDAFSEKYLAIEFTEQSSSDLIIGTINRQIDTVEKSISFIN
                     WFYDNYNEKLSAGSELSKVPFNLPNVAGDVQPSNKMYIGKEYGNELGDKLFQDTDYTA
                     LKKIDCSSFAEERDFLSFILRCGVSKYPKIYDNDSLKNDVGFKKFIYNKYKNEIKFTN
                     INYLTSKYISNFEELIDKLDTKDIVNWLNQDEELTDLLSSVKKESSASQQSNWSGLYF
                     NSNEYVKYILNKTAWIKIDGEKYSPRQIVLYEKLQAHVPGLYGVSEQSLMELLGQRIV
                     EQLDFRKNMAMFSDDEIRAILVELPKFDKGEISRRLYNELIRNKKGMNPTYSTEGLSV
                     LAKDGFFYPNKELRYADKRLPKSIEKQMRFIYVPEKASTTTIYEWLGVERFKTNLKLE
                     TFELLEDYSEDFKKEIDDIKTAVLSTIDDNSKNVSSLKRIQIIPCSKISAKDVEQDGQ
                     SIVLEDYYFVESKGKFYLKLPANSIEINQLRLIDTFSSTIVDIFKQVLNLSLDLNLIE
                     LLISRDTEHKQRKITEEFGVDKWNESFELLFSENALNKKVNDFLLSNGLNEEEYPTIF
                     EIDYSYALKEEEYQLLAIALESISKDIEDLNGLSELIHLDIREFWKLKLRSYLDKKAE
                     HYRQILYSNANKSGDEILKETFLEKIDRYKDYQFEISEIPNSVQVDIEKESKKRFPEL
                     SVSITDEIDIDGLYDNNVEELVTVAAITIDDFDYYIRGHQKVSSLLYFGIPSRLREDI
                     LIFVTKENDVDLNGEKSGISIEGTSTVQTILEPSNSVSRKGKNTRNGERSKRDYDNRN
                     SANDDAGETAEQIAYAELTQNQKLNVIWNTKYSSKSSDRNKQPPNGIVCDMWVHDPEN
                     GNMYFEVKSSITEFEMSINEYNSMLDNPDSYEVVLVNRDTKEISRHKFDELDGLKQAS
                     SYLFKFKQKRSDG"
     gene            complement(4968..6518)
                     /locus_tag="PWU81_000154"
     CDS             complement(4968..6518)
                     /locus_tag="PWU81_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1108893.1"
                     /translation="MAGKQVTVIPMKPRQWAAENTEEKPKLKVAAYCRVSTEMEEQAS
                     SYEAQVQHYTDYIQRNPDWELVGIFADEGISGTGTKKRDGFNRMIEACQNGEVEYIIT
                     KSISRFARNTVDCLQYIRQLKELKIAVFFEKENINTMDAKGEVLLTIMASLAQQESQS
                     LSQNTKMGVQYRFQQGQLRINHNHFLGYTKDEDGNLVIEPKEAEVIKRIFREYLEGSS
                     LQEIANGLMADGILTGGKRKLWRGEGVRLILRNEKYMGDALLQKTYTTDFLTKKRVKN
                     DGSYAQQYYVEDSHPAIIPKDIFMQAQQELDRRKSMKNKKSQCFSGKYALSGITVCGD
                     CGNAYRRVHWKNRGTVWRCKSRVDKREHNCTGRTIYETDLHGAVIKAINETLIDRDAF
                     LQQLNDNINSVMTDGLTDRLAELDSQLKELEAEIISLAVGGQGYDELASQIFSLRDER
                     DAVAKQIAANTNLQQRVDEMVVFVKEHDVINEYNEVLVRRLIEKVTIFEKNIVVDFKS
                     GVRVTVEI"
     gene            complement(6520..6969)
                     /locus_tag="PWU81_000155"
     CDS             complement(6520..6969)
                     /locus_tag="PWU81_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108894.1"
                     /translation="MRTTFEREVTRMNNTIPYGYVIKNGEINVDEQQAQQIREFFNIY
                     LEGVGLQEAGKRSGINKPHPFMKRILMNEVYLGNEIYPKIIDEDIFFQADLEQERRIK
                     FFKKRKEIKVPDTKVIPTHFKSGKITEKYSDPFKQAQYAYSKIKEVK"
     gene            complement(6933..8486)
                     /locus_tag="PWU81_000156"
     CDS             complement(6933..8486)
                     /locus_tag="PWU81_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1108895.1"
                     /translation="MKTIHKLARPELPEASKLKVAAYARVSTSSNEQLASLQTQITHY
                     ENHIKNHEQWDYVGVYYDEGISGTKTVKRDGLKRLIKDAELGKIDLILTKSISRFSRN
                     TVDCLNLVRQLTDIGVTIFFEKENINTGDMESELLLSILSSLAESESYSHSENLKWAI
                     RNRMSKGKFKAYPPYGYKRKGYDFHIVAAEAKVVKQMFQWALDGISTGMIAKRLNDGK
                     QFTKRGANWQATSVRSILKNEVYTGTLTMQQTFVDDQYKRRINKGELPMYQIKNNHEP
                     IVSKEDYDRVQELIRRKAEAKGNTGDGKKYTKRYAFSGNIICDKCGSKFKRVHTYWKG
                     NTKKVQWKCTGHLKNKDSCDALPITDEDLKVAYLTMLNKLIFGHKLVLEPFMKSLNKA
                     GANQEGLEVLADEIDKIQEKLEVLASLTASSILSTKVSLEEQSKLQQERAVAQEKQRL
                     IIESVNGKSTQQIELEALYKFARMAEMLTDWDEDLFKKFTNSITVYNRQEVGFELKCG
                     LLLKERLPE"
     gene            complement(8547..8720)
                     /locus_tag="PWU81_000157"
     CDS             complement(8547..8720)
                     /locus_tag="PWU81_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108896.1"
                     /translation="MTNKKTEINYSDEAAYYISQQILLNLLVTGKINLEEYVKIDNKN
                     RETFNPFLSRIMV"
     gene            9030..9320
                     /locus_tag="PWU81_000158"
     CDS             9030..9320
                     /locus_tag="PWU81_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108897.1"
                     /translation="MEGLEKEYEHFCKSATTKELEEFDESFKQEFEKCFKTIEAEEAL
                     YQFLQANINAMNNRMESIESPLFCINYKEQVFQLRNHLILMQNAVNTYLKEN"
     gene            complement(9381..10418)
                     /locus_tag="PWU81_000159"
     CDS             complement(9381..10418)
                     /locus_tag="PWU81_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HDL1108898.1"
                     /translation="MVTFKDLLPEPTESQQAFILDHGKTEDDGQLKYADDAKSYGWNM
                     RQYGKLKAGAVVLNRHPGKITKDRKWEIYGGGYVESISEPDEAGNVTAVITHAFTIEP
                     AIKQGDSFIENFDWNTPSKKKRKKPNSWAYFWDQYGMNEITYADFVALIENRHLIPID
                     DTQSLPSELDLTNEEVEEIEQESSKGFTVLVDEVGPNRTNGTQKRKFTGRHTDWERVN
                     KAKQKTGALGEEIVLDFLIQEAEKNKTKLPEHVSKTEGDGHGYDICAFDQSGNEIHIE
                     VKASKTNFSDGFEMSTNEVAASLGDTPYKIYFVHDLDVASKVCKIKIYDGPITEENFT
                     MVPTSYKIFNK"
     gene            complement(10534..11442)
                     /locus_tag="PWU81_000160"
     CDS             complement(10534..11442)
                     /locus_tag="PWU81_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL1108899.1"
                     /translation="MGISNLATKYGFQSFSHYSNGRSGAKVDKIVIHHMATTNYDIVP
                     DIWKTREASAHYGIGKNGEIRAYVDEGNTAWHAGNWNANISSIGIEHCNSTGSPNWGI
                     NQATIDASAKLCADIAKRYGLGKLVVNKNIFPHSHFSATSCPGQLLGKLQEIADKANA
                     INNGSSTPSTPASGLYRVRKSWADAKSQIGAYKDLNNAKRAADSHSGYKVFDQNGNQV
                     YPSGSSTSNVKTLVVQVSGLNVRNKPSLSGAVVGSYNKGATWTLPKSEKMTIADGWVW
                     ARCPIGYCAVGKNTGKTESDDYILIS"
     gene            complement(11432..11851)
                     /locus_tag="PWU81_000161"
     CDS             complement(11432..11851)
                     /locus_tag="PWU81_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL1108900.1"
                     /translation="MKEVWNWIQLAIAGGGGVIGWYVGGIDGLIYTLVTFVVVDYVTG
                     LLRAINEKKLSSKIGAKGITKKIMIFLLVGVAHMLDVEVIKGGNVLRDAVIFFYISNE
                     GISLLENAVAIGLPVPEKLREILQQLHEKKEETENGN"
     gene            complement(11852..12259)
                     /locus_tag="PWU81_000162"
     CDS             complement(11852..12259)
                     /locus_tag="PWU81_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HDL1108901.1"
                     /translation="MLKIRNEDLVDVMSFLKGFDLPPKISRVRTKLAKLIQVKVDELY
                     QDEVDLLQKYGKKDEHGNLVQHDGAFSLIPETATEYHKEKADLLNEETHINVDELQDK
                     LPLLVAGFENSEVTISGKDAEMLDLILEQLEDL"
     gene            complement(12246..12413)
                     /locus_tag="PWU81_000163"
     CDS             complement(12246..12413)
                     /locus_tag="PWU81_000163"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108902.1"
                     /translation="MNEEKEEVIVTEPIEADIPKPMKVGTDMVDLLVMVVDDLCVQIG
                     DLKEKIDNAQN"
     gene            complement(12423..16313)
                     /locus_tag="PWU81_000164"
     CDS             complement(12423..16313)
                     /locus_tag="PWU81_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1108903.1"
                     /translation="MITLYNKTESNFNHNGLAVLDGNILNPVVSEELNGLFSFEFDYP
                     IHAPHSEELLPEMIVKAPVPELSDQLFRIVERDDALGGFLHIVAHHIFYDLATNFIED
                     TYIVNKNGSGALTQLLGATQFMHRFTGTSNISAVNNVRLVRLNPVEVLLDSDLENGFQ
                     SSYGGEIVRDNFTISMLTTRGSNNGVQIRDKKNLTGYKADVDYSGIVTRIMPQGYDGL
                     FLPEKYVDSPNINQYVQPKIKVIKYDNVKVGDEEDEFTQAEAYDELRRLAGLEYSANK
                     VDLPKATYDVEFAPLERTEEYKDFAQLETINLGDTVSVIHAEDGLNITARMVSYKYDP
                     FLKAYISVTLGNITPKFTDVAKDINRVDNKAEQAKDDANYALSSANGKNTNFYGPTTP
                     NNPKLGDVWYKENGDKFEMWVYEIRDGVTQWYPLMTDMTAEEVKQAVEQAQKDANTAT
                     EKANQAFNDAVNALNAAESVGASVETLTTSVEQNTSKISLVASGMSTLEGRVITAEST
                     LTVQAGQIASKASQSTVDNLTGRITTAESTITQNADRFELSLSKTNKAVNVLAGMSIA
                     DLQWAVGGLNTNDGKENTYGGYVRSGFTRVRNGEKYLLQTFVGGKLYSTYATAYLLYY
                     KEDKSFLSYTQFGNTTTPLTVPSSAAFIRIRLTTSDAPETISCYMLKTDVTNGYVDLS
                     EISNVVKLTATTDALLLSVSETKEAIGVPFKVNQWEQGSLSTTDGSEVSATNYLRSEF
                     LDVKVGDKYIGQLRDGTQAAFYYHYYGMDLPYIDFVPTAKQFSETLTAGTYTNATIAS
                     YLVSKNVESMSITSGVTTNPYSNSGDYLVIYKLPLEGKVAPSVISWNGRKDTSDNIVL
                     LYTGGAWEQISTVTSSSNATYSWTLTAEQISGITTDSVYLAFFSVKTGTYAGIYNSSN
                     TPFTLNPSDGSSYHQISYTGSSGAITVPSGTLKMRVRVNTSIKPDEYEGNIYPTTTRQ
                     DYTNANTLYSAILMQKDLINLRVGKGDVINQINISPESILIAGNKVHITGQTTIDSAV
                     ISSAMIQNAAITNAKIADATIQSAKIASIDAAKITTGTLSAARIGANSITADKIAANI
                     LTALTASSSIRITGTTIGYYSGSTLVTEINSQGMTIRRGGTTVGTIGANNISGHSDWR
                     GLVFDLEYGTEYMSWAHKDSSSASVYTQKLSWYARTLQSGMEKGFHFDDDIYLPSMLR
                     IGTQHQISVGGITFSSSLYTKIGSSSGSAGIAFNGSNLMLGDDGKWVDFGVIREICAK
                     LAGRTIALPTGFSSNGTASGWYNPQSFNSMTTYS"
     gene            complement(16310..17002)
                     /locus_tag="PWU81_000165"
     CDS             complement(16310..17002)
                     /locus_tag="PWU81_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1108904.1"
                     /translation="MNAFILDGTINSRTDLGLRITEVPVIPVTERVIDSIEIDGREGD
                     LTILKGWKDVTFGFKVVIWKSDVWTSWQIILPQILNAKTIAFSNEPNVFYPIKYAKAS
                     GLTQILSSMWEFEMELTCSPFRYKTNVATINRTTSGTVNNPGNIYSLPKIKVYGNGTR
                     TLTINGKPIVLNLTNGNLTLDSDLKECFYGDVAANQYMTGDFPEFRVGSNTVTLGTGI
                     TKVEIEPRWRYL"
     gene            complement(17079..17480)
                     /locus_tag="PWU81_000166"
     CDS             complement(17079..17480)
                     /locus_tag="PWU81_000166"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108905.1"
                     /translation="MLKHKFKAKKPALIGYILLLAIGLFLTAVRWYSGINHGFVFINN
                     QINSHISNFALSFLFYLVIGYLWLLMGTNLRVLTAMGAGLIVANIVCETMMTFLNTPD
                     LIDAIYGISGVLVVYAYLAITKKIGLEETTE"
     gene            complement(17551..20112)
                     /locus_tag="PWU81_000167"
     CDS             complement(17551..20112)
                     /locus_tag="PWU81_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1108906.1"
                     /translation="MADNFGLKIGVEGEKEFKNSLREINQSFKVLGSEMKLVSSEFDK
                     NDKSISSLSARNQVLNKSIDAQKEKITTLENALKNASDSFGENDKRTQNWTVQLNNAK
                     AQLNNMEREVANNEKAIDAMGTEEVDAAKKTDELGNELKDTGDEAEKSGSKFEKFGGV
                     LKGIGVAMGTVALAAGAAAVELGKQVVSAYADYEQLVGGVDTLFKDASSTVQKNADNA
                     FKTAGMSANEYMETVTGFSAALISSLGGDTKKAAGVADQAVTDMSDNANKMGTDIESI
                     QNAYQGFAKQNYGMLDNLKLGFGGTKSEMERLLQEAEKISGVKYDISNFADVTEAIHV
                     IQTEMGITGTTAKEASETISGSIDGMKSAISNLMAGLGNSSADIGMLIGNVVEQFQNV
                     IKNILPVIQNIVSALPAVVDAIIQALGQLLPTLLEAVTSLFSQVLETILGLLPTLIPV
                     VVQAVLTIVQTLIENLPLLIDAAFQLVTTLIQGLGEALPELIPITVQAIITIVNGLIE
                     NLPLLLDAALQLIMGLAQGLITALPILIESLPQIITGIVNFIISAIPQIIQTGIQLLT
                     SLVTALPDIINAIVAAIPVIIQNIINAVIGAIPQLIDAGIQLLVALIGALPQIITTIA
                     NAMPQIINGITGTLVNNIDKIINAGVKLLVALVQNLPAIISAIVKAVPQIITGLANAF
                     GEYVSTMGTIGKNLITGLWNGIADAGSWLRNKISGFFGGVVDNIKDFFGIHSPSTLFR
                     DEIGKYMAQGIGVGFDKEMTGVSKTMQKAIPTKLYMPSFDIDTDIHAAVDSTFSMLDL
                     GAKVDALTNITSEMLPTLLKAMDIKVVLNDGTLVGKLAPEIDKSLATLNRRRVAY"
     gene            complement(20319..20699)
                     /locus_tag="PWU81_000168"
     CDS             complement(20319..20699)
                     /locus_tag="PWU81_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108907.1"
                     /translation="MSEERSAKISLGGDEYELILTTKATKEIASHYGGLDNLGEKLLK
                     SENFELALDEIIWLITLLANQSIKIYNLKNTEKKDELTTEYVELMTSPLQLAEYKSAI
                     TEAMFKGTARNIESETDSKNKAGE"
     gene            complement(20709..21299)
                     /locus_tag="PWU81_000169"
     CDS             complement(20709..21299)
                     /locus_tag="PWU81_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019155109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1108908.1"
                     /translation="MATIGLDKLYYAPITESATTGEETYGTPAMLAKAISAELSVELA
                     EATLYADDGASEVVKEFKNGKLTLGVDDIGRAVAAVLVGATVDQNGVLISTTEDGGTP
                     VAIGFRARKSNGKYKYLWLYRVKFAVPSTSLATKGDDITFSTPTIEGTVMRRNKLDAS
                     GKHPWKAEVDENDTGVEQTTINGWYTSVYEPDFTAD"
     gene            complement(21302..21643)
                     /locus_tag="PWU81_000170"
     CDS             complement(21302..21643)
                     /locus_tag="PWU81_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108909.1"
                     /translation="MNILEELNSLFATLHIPVETGEFSDKAPDKYCVLTPLADRFDVY
                     GDNFPTVDVQEVRISLFVKGNYLNTQKQLVQNLLKHEFTITERLFVGFEKDTNYYHVA
                     IDVAKHYEMEV"
     gene            complement(21640..22071)
                     /locus_tag="PWU81_000171"
     CDS             complement(21640..22071)
                     /locus_tag="PWU81_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL1108910.1"
                     /translation="MAKALMKMPEDFLLKVSKLENKTDEIIPKVLESGAEVVEGKVRS
                     NLTAVVGSNTKVPSRSTGELESALGITQARQDKSGNWNIKVGFDEPRIDGSSNAKIAN
                     ILEYGKHGQPAKPFLKPAKSQSRKSCIETMKSKMESEVNGI"
     gene            complement(22064..22402)
                     /locus_tag="PWU81_000172"
     CDS             complement(22064..22402)
                     /locus_tag="PWU81_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL1108911.1"
                     /translation="MSFGKMNQRIQIVKVTNQKDSAGFSVKQDEVLAAVHAYKEEKNA
                     TEKWVNMATFSSATCLFRIRYIPNLEVTTEMVVISDQKRYQIVSVENVRNRNMYLEML
                     TTEMEVSADG"
     gene            complement(22399..22710)
                     /locus_tag="PWU81_000173"
     CDS             complement(22399..22710)
                     /locus_tag="PWU81_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL1108912.1"
                     /translation="MSDLLNKVKANLILEHNEDDELLERLILTALSYAESYQHIESGY
                     YSEHEMPPTTEQAVIVLASNFYESRDGSTGGFFADNVNASKQVWETVNLLLRLDRRWQ
                     V"
     gene            complement(22721..23932)
                     /locus_tag="PWU81_000174"
     CDS             complement(22721..23932)
                     /locus_tag="PWU81_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL1108913.1"
                     /translation="MNKIQELMEKRAQAWNGAKAFVEAKQDKDGLMSEEDAKTYAEME
                     TKVKNFTMEIERMQSMEAMDRELEKPMSQPLTSQPMTTENKMEPKKTGRASAEYKEGV
                     LEALRSNFRHVSNVLQEGVDTAGGYLVPEEYDSRLIDGMTSENIMRTLATTITTSGEH
                     KINIAGSKPAASWIEEGGELSFGDATFDQILLDAHKLHVAIKVTDELLYDNAFNLENY
                     IIDQFTKALANAEEDAFLNGDGVGKPLGIFAATGGGQVAITTNTQSAVTADDVVNLVY
                     ALKRPYRKNAKFIMNDQTISLLRKLKDNNGAYMWQPALVAGEPDRLLGYPVYTSEFVP
                     TVKAGAPVIAFGDFSYYNIGDRGVRSFDQLRELFAGNGMVGFLAKERVDGKLVLPEAV
                     QILKMKASASS"
     gene            complement(23936..24691)
                     /locus_tag="PWU81_000175"
     CDS             complement(23936..24691)
                     /locus_tag="PWU81_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL1108914.1"
                     /translation="MKKFWKWKNQTVLNQDNQEEILERTLYLNGPIAEESWFDDEVTP
                     QIFKDELNSGGGNVTVWINSGGGDCVAAASIYNMLADYKGKVTVKIDGIAASAASVIA
                     MAGDKVLMSPVAMVMIHNPSAVAFGEKVDMEKAIAMLDEVKASIINAYERKTNLSRVQ
                     LAHMMDAETWMDANKAVHLGFADEIIGEIDPDIPVNNSMYSEVKVTNSLTSAIAKKCR
                     IEPPEPKEREEINNTIPADSLMSRLNLMKNWRN"
     gene            complement(24591..25919)
                     /locus_tag="PWU81_000176"
     CDS             complement(24591..25919)
                     /locus_tag="PWU81_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL1108915.1"
                     /translation="MGIIKSLFKSRDKPTNQTIMNPYRFMFGGSSAGKSVTEQTAMQM
                     TAVYSCVRVLSEAVAGLPLHFYQYRDAGGKDKAMKHSLYFLLHDEPNSEMTSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVVALYPLMPDKMTVNRDDNKEIYYLYQADVGQVRLS
                     KADVLHIPGLGFDGLVGYSPIAMAKNAIGLAIATEEYGSKFFANGANPGGVLEHPGTL
                     KDPGKLRESWNATFGGSSNAAKVAVLEEGMKYTPISMSPEQAQFLETRKFQLEEIARI
                     FRVPLHMVGDLDHATFSNVEHLSLDFVKFSLSPWLNRWEQALNKSLLSRDEKEKYFIK
                     FNVDGLLRGDYATRQQGYATGIQNGFLSPDDVRELEDLNPIPGGAGAKYYFNGNMLPI
                     EQAGAAYGVNKEESEEQDEEVLEVEESNGSESRQPRGNPRENPLPQRTNR"
     gene            complement(25985..26719)
                     /locus_tag="PWU81_000177"
     CDS             complement(25985..26719)
                     /locus_tag="PWU81_000177"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108916.1"
                     /translation="MNEYLIQVAPDKISSDPDNTLISRLFYRKWWGKILTEDLTKYAE
                     GIGNYRGETIISFNTIAGATIRLLKCYNQKNFSMPDSHYGRLKVILYSDDITEEMKVK
                     FVKFYDLSHTLANFMPLYVSKPSLNGVKGKGPYHDFPDLFYSDVRDKRYGSSTNADKF
                     IVNNKDYFSKFKDWKSFIELNYLQDFFDDEDYMTFKKLAPSSSFFPYQRKSLTPDEKS
                     KCLEEVNTFLNNAIEIILSRGSRFYI"
     gene            complement(26775..28340)
                     /locus_tag="PWU81_000178"
     CDS             complement(26775..28340)
                     /locus_tag="PWU81_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL1108917.1"
                     /translation="MDKDSHYDKSRADYAVAFIELLKHTKGQWFGKPFKLMPWQEKII
                     RDLFGTIKANGYRQFNTAYVEIPKKNGKSELAAAVALLLTCGDGEERAEVYGCAADRA
                     QASIVFDVAVDMIRQNPALSKRVKILTSQKRIIYQPTNSFYQVLSAEAYSKHGLNSSA
                     VVFDELHAQPNRKLFDVMTKGAGDARTQPLFFLITTAGNDTNSICYEQHQKALDIIEG
                     RKHDKTFYPLIYGIKQDDDWTDPEVWKKANPSLGITIAEEKVVAACDSAKQNPAEENS
                     FRQLRLNQWVKQAVRWMPMDKWDACSFNADEESLRGRVCYGGLDLSSTTDLTSFVLVF
                     PPEDEDDKFVILPYYWVPEDTLDLRVKRDHVNYDLWAKQGYIQTTEGNVVHYGFIEKF
                     IEELGEKYNIREIAFDRWGAVQMVQNLEGMGFTVVPFGQGFKDMSPATKELMKLTLEQ
                     KLAHGGHPVLRWNMDNIFVRTDPAGNIKADKEKSTEKIDGAIATIMALDRAIRCGNDN
                     GASVYDDRGLLFL"
     gene            complement(28423..28566)
                     /locus_tag="PWU81_000179"
     CDS             complement(28423..28566)
                     /locus_tag="PWU81_000179"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108918.1"
                     /translation="MMDYQIYMPDNEDGIEEGYYDWQELVQLLRDNKNNPEAIQFIAD
                     MME"
     gene            complement(28563..28739)
                     /locus_tag="PWU81_000180"
     CDS             complement(28563..28739)
                     /locus_tag="PWU81_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5049 domain-containing protein"
                     /protein_id="HDL1108919.1"
                     /translation="MNAKIKQQIIEIAESGLTNMFDAIAVQRIAHDRHFYELVIFIEE
                     HKDEYSRFILEGDK"
     gene            complement(28723..28917)
                     /locus_tag="PWU81_000181"
     CDS             complement(28723..28917)
                     /locus_tag="PWU81_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108920.1"
                     /translation="MKVSKRKIYNIAKNYIVQLPERGDLKAHNSDREDFLDIAVWCLE
                     DALIAAYEAGRKDGQNERKD"
     gene            complement(28919..29296)
                     /locus_tag="PWU81_000182"
     CDS             complement(28919..29296)
                     /locus_tag="PWU81_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HDL1108921.1"
                     /translation="MFPTHEQIEGMKKRYPEGSRVELIKMNDPHAPAKGTQGTVTGVD
                     DIGSILIQWDNGSTLNAVYGEDVVHIIKPDFELVFQNGRREGFETFNDAWQYVTDMIL
                     NHDLVWVDLESKTEDTIYIRKGL"
     gene            complement(29370..30605)
                     /locus_tag="PWU81_000183"
     CDS             complement(29370..30605)
                     /locus_tag="PWU81_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019116086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL1108922.1"
                     /translation="MNTTERFEKVDIDKLVPYARNARTHSKDQVKQIQASMREFGFIN
                     PVIVDKDYNLIAGHGRILAAKEEGIKQVPCVFVEHLTEAQKRAYILADNRLAMNAGWD
                     DEMLAVELSDLQGEEFDLDLLGFETDELDKLLNGNDDAQDDDFDVDEELEKPAFSKTG
                     DLWLLGRHRLVCGDSTKPETYEKLMDGKLANLVVTDPPYGVSYQGKAGTIKNDNLKDE
                     AFYNFLYDAFSCMESVMANDASIYVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLV
                     LGRSPYQWQHEPVLYGWKKKGKHQWYTGRKESTIWEFDKPKKNGDHPTMKPIPLLAYP
                     ITNSSLSNCIVLDSFGGSGSTLIACEQTDRICHTIELDEKFCDVIVKRYIEQAGSDAD
                     VSVIRDGKTIAFNDLENPE"
     gene            complement(30605..31387)
                     /locus_tag="PWU81_000184"
     CDS             complement(30605..31387)
                     /locus_tag="PWU81_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase"
                     /protein_id="HDL1108923.1"
                     /translation="MIEKVNPQHPDKVADRIAGAIVDLAYAEENNPKIAVEVLIGHGI
                     CHVIIETSTALNLNEISQAINRIAGKIKKDVVIVPQDKELADNQATEIRCGDNGIFRG
                     MPLTDEQLQLSKIARDLYQDHPFDGKYILDGERLIICQSHAKTNELKQKYPNATVNPL
                     GDWTGGPDVDTGATNRKLGSDMADGVTGGGIHGKDLSKADVSVNIYAFLKAQETGMPV
                     ELSCAIGDTEIDGVPYSEIVEVARDYVNELGGFEKLAEWGLF"
     gene            complement(31402..31974)
                     /locus_tag="PWU81_000185"
     CDS             complement(31402..31974)
                     /locus_tag="PWU81_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDL1108924.1"
                     /translation="MAKDGTQRGGRRPRSGERSQPLVDKVAAGKKASRMEFDLPDVEL
                     LIGEDIGEGVELEGNDYPEPSAYLSAKQKDGTVLGADEIYNSTFQWLKDRGCETLIAP
                     RMVESYAQNFARFVQCENALSQYGLLGKHPTTKAVMASPFVAMSQNFQKQANVTWYEI
                     QQIVKENASVEYTGDPNTDAMERLLRSKNG"
     gene            complement(32108..32467)
                     /locus_tag="PWU81_000186"
     CDS             complement(32108..32467)
                     /locus_tag="PWU81_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL1108925.1"
                     /translation="MKPANPCKFPGCPNRTHDTYCDEHKTLARKRYEKYERDPAINKR
                     YGRAWKKIRARYVAAHPLCEMCLKEGRFTSVEIVHHIKELSDGGTHDENNLMSVCKSC
                     HSKIHMTRRNTKDKTIM"
     gene            complement(32641..33069)
                     /locus_tag="PWU81_000187"
     CDS             complement(32641..33069)
                     /locus_tag="PWU81_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HDL1108926.1"
                     /translation="MTPKEYLKQAYRLNKRIDDRIKEKAELYQLAVTVSSTTFEEKIS
                     SGTRNTEAPFVRNLEKCYQLEAEIDAEIDKFVDLKRQMRGVIDELTNVDEKIVLINRY
                     LLEESWDKIAEDLHADVRTVFRWHSKALENLKLPDEPIVI"
     gene            complement(33066..34418)
                     /locus_tag="PWU81_000188"
     CDS             complement(33066..34418)
                     /locus_tag="PWU81_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL1108927.1"
                     /translation="MKFVPHNYQKYAIDFIKDHKTAALLLDMGLGKTVTTLTAIKDLM
                     HDDFTIQRVLIIAPLRVTQSTWPTEIQKWDHLKDLSYSVVLGTPSQRMAALQNNANIY
                     LINRENLDWLINKSGFTFDFDMVVIDELSSFKNLKAKRFTSFMKVRHKVDRIVGLTGT
                     PSSNGLMDLFAEFKVLDMGERLGYYISRYRDKYFLPDKQNGMQIYSWKPREDAEQEIY
                     DKISDITISMKSVDFLDMPELVMNEVPVDMGLKEKQKYDEIKADLVLQLKEATIDAAN
                     TAVLSNKLLQMANGAIYDEFNVSHHIHDQKLDALEDLIEGTNGKPILIAYWFQHDLER
                     IKERFNVRQIKTSQDIIDWNEGSIPIAVIHPASAGHGLNLQAGGSTLVWFGLTWSLEL
                     YQQTNARLWRQGQSDTVVIHHIIAKDTIDEDVMLALKRKDKTQSCLIDAVKARLEGSK
                     "
     gene            complement(34418..34702)
                     /locus_tag="PWU81_000189"
     CDS             complement(34418..34702)
                     /locus_tag="PWU81_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HDL1108928.1"
                     /translation="MREKQVEQALVKAVKRVGGICPKFTSPGLAGVPDRLVLMPNGKL
                     GFVEVKAPGKKPRSLQLFRMKQLTDLGFQCFVLDEIDQIPELLERIGGDV"
     gene            complement(34699..34908)
                     /locus_tag="PWU81_000190"
     CDS             complement(34699..34908)
                     /locus_tag="PWU81_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108929.1"
                     /translation="MKFKQWLQIGIGKRDLRFDEFYEFAMSDVTYPWKKSFDKQVAHL
                     MIHCAEKEYLEILRDAYFEYLTVWL"
     gene            complement(35046..37289)
                     /locus_tag="PWU81_000191"
     CDS             complement(35046..37289)
                     /locus_tag="PWU81_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL1108930.1"
                     /translation="MLKMTLFTSNQVGNSKNGLFPTKQVVTDKETLVATVKLDHVMAE
                     YKGNYRKGDNFIQSDVIPLDCDNDHSDNPDDWVAPFEVAMQFPDVTFAVSYSRNNMKD
                     KGGKSARPRFHVYFPIPATTDKDEYVELKKQIQSEFPYFDNNAMDAARLLFGTPNSEV
                     EIYEGSKLITDFLEDDLFADWDNAQSEISEGSRNSSMSHIAGKLIKRYGATEDTYHKY
                     LEQSQKCNPPLPEEELKAIWNSAVNFGKKVKQQEGYIPPEQYNSDLSLEPTDYSDLGQ
                     ATVLAREYECKLHYSPSTGYLVYNGSYWEESKPKAQGVIHALTERQLEESETEIEKRT
                     KEMVSNGAFDVLASVGPKKAVTMFNTAQRHSFDLYQHAQEYKKFAVKRRDSKYLSSAL
                     TEAKPMLEIEQRLLDVNEFLLNTPTATFDLRTGKSQDHNSEDYITKQTECAPSDANQQ
                     IWLDALNTIFVGDQELIDYVQMIVGLAAIGKVYVEALIISYGEGRNGKSTFWNVISRV
                     LGNYSGSISADILTSQIRRNVKPELAEAKGKRLLIAAELEEGMRLNTSNIKQLCSTDE
                     IAAEKKYKDPFKYVPTHTLVLYTNHLPKVGAIDKGTWRRLIVIPFLATIDGNKDIKNY
                     ADYLFENAGGAILQWILDGAKKVIAADFHLTVPQVVANAISEYKTANDWLGHFLDECC
                     ETAVDFEEKSGELYAEYRAFCTRTGEYIRSSADFYTALEAGGFKRRRTNKGNKIVGLQ
                     LKSEFLE"
     gene            complement(37282..37626)
                     /locus_tag="PWU81_000192"
     CDS             complement(37282..37626)
                     /locus_tag="PWU81_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HDL1108931.1"
                     /translation="MPRRKDYRTSVYVCSPFSGNVVVNHKNTKRYCRIALDRGYMPVA
                     AHLMYPQFMDDATERDVAMHMSLVLMGKCEEVWVVGDTISQGMAVEIEQAEYWKKHIR
                     YFDDEMKEVSNA"
     gene            complement(37619..38389)
                     /locus_tag="PWU81_000193"
     CDS             complement(37619..38389)
                     /locus_tag="PWU81_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDL1108932.1"
                     /translation="MNELKVFENAEFGSVRTVMIGDVPHFVGKDVASILGYTNPQKAL
                     RDHVDEEDKTVNESFSVNGTMAILINESGLYSLIISSKMPNAKKFKRWVTNEVLPSIR
                     QHGVYATEDFITKSIEDPAWAISVLKQLQEKKEMVAMQQQMILEMKPKVSYYDLILQN
                     NSVMSISKISKDYGMSPQAMNKLLHELGIQYKQGKMWLLYQKYANQGYTQSKTHAIDA
                     ERSVMHTYWTQKGRLFLYDLLKSKKNILPVIERGDNNA"
     gene            complement(38599..40536)
                     /locus_tag="PWU81_000194"
     CDS             complement(38599..40536)
                     /locus_tag="PWU81_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HDL1108933.1"
                     /translation="MKHLSLDLETYSSVDLSKCGVYKYSESPDFEILLFAYSVDGGPV
                     EVVDLKMDETIPAEILTSLSDETVIKHAFNASFERVCLSRFLGLDGFLNPRGWQCTMV
                     WGATLGLPLSLVGVGAVLGLDKQKLTEGKDLIRYFCVPCNPTKVNGGRTRNLPHHAPT
                     KWSAFKTYNSRDVEVEIAIQGKLSNFPVLDSVWKEYWLDQEINDRGIEIDKDFVEAAV
                     QLDELSKAGLNQQLQELTGLENPNSVLQMRGWLLDHGLETESLGKKAVAELLEQPIDE
                     NLKQVLVLRQQLAKSSVKKYQAMQNFACSDNRSRGLFQFYGANRTGRFAGRGIQLQNL
                     PQNHMSDLSEARSLVKQSNFEMLNILYGNVPNVLSELLRTAFVPREGMKFIVSDFSAI
                     EARVIAWLSGEKWRQEVFKNGGDIYCASASQMFGVPVEKHGVNGHLRQKGKIAELALG
                     YGGSVGALKAMDALEMGLTEDELQPLVNSWRDSNINITELWWAVDKAVKTCVKERRST
                     ATHGIKFEYKSGFLFIQLLSGRKLVYVKPKIGENKFGGEAVTYEGVGATKKWERLESY
                     GPKFVENIVQAIARDILCFAMENLRKYRIVGHVHDEVIIEVPMESKVEDICQLMSQTP
                     KWAEGLLLNADGYECEFYQKD"
     gene            complement(40597..41139)
                     /locus_tag="PWU81_000195"
     CDS             complement(40597..41139)
                     /locus_tag="PWU81_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HDL1108934.1"
                     /translation="MSKTSKVITGTNTRLSYFHGWEPASINGGAEKYSTAVLIPKSDK
                     RTIDTIEKAIDAAIEEGLAKFGGKKPNKAEIKLPLRDGDIERDDEAYQGHYFINANST
                     TAPQIVDQHVQPILDRSQVYSGCYARVSINFYAFNANGNRGVAAGLGNIQFVRDGEPL
                     GSQVSASDEFETLADDDFLA"
     gene            complement(41141..42283)
                     /locus_tag="PWU81_000196"
     CDS             complement(41141..42283)
                     /locus_tag="PWU81_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HDL1108935.1"
                     /translation="MPTQHAVLSASSSNRWIHCPPSVRLSEHFENKSSPYAQQGTDAH
                     TLCEYKLNKFIGNDVTDPTSTLEFYDEEMEQCAESYATFVMEEVAKAKQTTVDSIVIV
                     EQRLDFSRYVPDGFGTGDCLVIADGTLSVIDMKYGLGILVDAYQNPQMMCYALGALEL
                     FDVIYDIQEVKMTIFQPRRENVSTYTLLKEELLQWAKDVLSPAADLAFKGEGEYQCGK
                     WCGFCPAKNSCRARADHNLKLAQYEFKPPELLSDDEIEEIIGKVDDLVSWSNDIKDCA
                     LKLALGGKQWTHHKLVEGRSTRKYSNDNDVAAAVIKAGYDPYDKKLLGVTAMTKALGK
                     EKFNEILGEYIIKPKGKLTLVDGSDKRQAVTVNNLNEEFKPLTEEQ"
     gene            complement(42284..42598)
                     /locus_tag="PWU81_000197"
     CDS             complement(42284..42598)
                     /locus_tag="PWU81_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein rrp5"
                     /protein_id="HDL1108936.1"
                     /translation="MSKTKLFLQVVEDVQALADSLQELAVAMKTNEPEPQVETPEPTM
                     ETKSEVKQPTLEEVRGLLARKSQEGKSTEVKALIEKYGASRLSDVPTENYAALVADAE
                     GL"
     gene            complement(42603..42809)
                     /locus_tag="PWU81_000198"
     CDS             complement(42603..42809)
                     /locus_tag="PWU81_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108937.1"
                     /translation="MNLKHKIRVNVTDDTGNKETVLQGGVRHLPRWFARWLFGKGNMD
                     KILVLTPGDTIESVEIREVNKKGA"
     gene            complement(42906..43523)
                     /locus_tag="PWU81_000199"
     CDS             complement(42906..43523)
                     /locus_tag="PWU81_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108938.1"
                     /translation="MSRFMKTHQNDRSTYKYVFERTDANGRIQKEVVEIRPGEDGVTE
                     LNIQELHRADDRAIYNFYKNARPERTEEEKEKIKAWKEKFREDFNNEHGYYPMDEYVD
                     GEANDYFKRNYNLSLNEFDDECDEGDNPIQSLVYDAYQDNEEDPRIDRLREVVTQLLP
                     YHQEIYHMLVTENMSQTEVADKLDKKKQSINRTFGIIKDKIKELF"
     gene            43918..44868
                     /locus_tag="PWU81_000200"
     CDS             43918..44868
                     /locus_tag="PWU81_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108939.1"
                     /translation="MEISEITQTLYPFMGSEYVKPQDFLIALLDSVSNDDEATIFGFS
                     DDNLRRFYNGTRPLTQEYAVRVAGKFNIDKCTTFIYERLIDDAPTVLKQQLIDKGVVI
                     PKDTDVSEIVAHLLLDEITSIASQTKVKTKKAKPKLSELELASLRINTNGDFSYNDEV
                     KKFFDDLPVPLDIRAEEMTYVIALFSAYADADGYEVYEHTNDLPNSRLKDFKRQRRNY
                     YKAESIRRGVRDNFTQEEGTEHFEALKEDMYDGIEEVYEEDYDDGVERLKAVLQQSSV
                     ITLNGSILSFIPGLLQNSAKKGICHMLVNDGKIKWVTEDE"
     gene            44861..45307
                     /locus_tag="PWU81_000201"
     CDS             44861..45307
                     /locus_tag="PWU81_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108940.1"
                     /translation="MNSLFNTPFEISVRVMLLLSEFKEPVSADRILITDFITTYGRDF
                     EISDYNLNGDNSYRFSEFIARRELVKDAVKNLVLQHVIQPSQSKEGFKYALSEDGLKL
                     ISQFVSVYAEKYLQIADESVKYIQDKSDVELLRQINQRAKEFKGEQ"
     gene            45310..47142
                     /locus_tag="PWU81_000202"
     CDS             45310..47142
                     /locus_tag="PWU81_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1108941.1"
                     /translation="MQNYYIERVTIKGKDREDATVKFTNGLNIISGPSNTGKTSIVTA
                     IDFLYGAKKDAAPFSTDATGYSKVELIIQSTNGKYSLTREFDSNSILVSKITSSGEYV
                     DYEKYNARSGKNPISHFWLQLLGIDDDYQIIKNEDFKRQKLTWRTLAPSTLIPQDRVN
                     TSASVLFPGETTQRTAFLSSLLLLLYGSDFANSEENETKEHKKIRRNSVINYINSSLS
                     ELYENYEVAKSELPEAPSDDFQQIVDGMFAELETIEKQINDALASSKILIKEIYQKEQ
                     YLHETQLLLNRYKQLHYQYQSDIERLTLIVDGERLLNDHNEHDTSHSCPFCNGELSKP
                     VQTTYIDTARSELHSLLSQLSDLTEESHDTEVELQELMEQLDELKTRKKSLDAAINDE
                     LKPKAETLKDRINKIETYRDIKQQLISMEKMQNKLKSDLEKIENEPEDERILYKPKEK
                     FDAEFYKEMSELLDSMLRNMHYKPNEFRNAFFAKDIFDIKINGNLKWTENGHGYTAFI
                     NSVVVMAYRHLLYNHAKYTPSVFIIDSPLLGLDERKKQDGDDNMKEGLFKYFMDHQNE
                     GQLIIVENTTNLPALNYDDKQVNQIVFTEDKNNGRYGYLYDIYN"
     gene            47158..47361
                     /locus_tag="PWU81_000203"
     CDS             47158..47361
                     /locus_tag="PWU81_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1108942.1"
                     /translation="MKFSYNPLWKLLIDRGLNKAQLRKMTDISSATLSKLNKGENVTT
                     DILLRICEALDCDLSDIVEIVRD"
     gene            complement(47412..48548)
                     /locus_tag="PWU81_000204"
     CDS             complement(47412..48548)
                     /locus_tag="PWU81_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL1108943.1"
                     /translation="MANSLDSIFEDDAFDDLTKQVEKKKVVKQDPEVAKFQEIIDFVN
                     ENGREPEKTPEWSSERALWARLSGFRGKPERSEKVKKYDTLGLLEKEFEPDGEFLADS
                     VEIDDELNLDDILGSDDMIDDFSDLLDVSRYRRTVNAADKIGRRKHASHFEKYKSLFD
                     QVHADIASGRRKIIPFEQYDIHENRFYIQNGVMMYIVSISKDTFIADNGKTNARMHVV
                     YENGTENKNLLLQSLASSLYSTERHGRMVTEPVDEETLAESFGEEFTTGYIYVLKSLS
                     TNPDISKWNNLYKIGFTRNDVESRIANAENDVTYLNAPVKVMLSAEVQNVNAQLLERT
                     LHHVFQDKQVVFQDGNFKKATEWYMVPLNDIESKINEVIAGLQK"
     gene            complement(48538..50493)
                     /locus_tag="PWU81_000205"
     CDS             complement(48538..50493)
                     /locus_tag="PWU81_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL1108944.1"
                     /translation="MTSNLFEINFDKNQSKTTNDLGMREMQERVYEKRASQYLLVKSP
                     PASGKSRALMFVGLDKLHNQGIKKVVIAVPERSIGKSFKDTELSKFGFYWDWEVKPSD
                     NLTIGGGEKSKVQHFVKFMNSTDPSDNILIATHATLRFAFEELDDSLFDNTLLAIDEF
                     HHVSRDDKSVLGNALRSIMSNSNAHILAMTGSYFRGDSVQILEPADEDKFDKVTYTYY
                     EQLEGYKYLKSFAMGYSFYRGKYTDALDKVIDVNKKSIIHIPNVNSGESTKDKYAEVD
                     EILDLLTDGGNITQNEDGIYEIERPDGKKLLVADLVNEEGREKVSAYLASITDDIKDL
                     DKLDIIIALGMAKEGFDWPFAEYALTIGYRNSLTEVIQIIGRVTRDSPNKTHAQFTNL
                     IAQPDAQDDEVLFAVNSIMKAISASLLMEQVLAPVYKFKPKDGKPDKPGDLSIRGLKP
                     ADTTERTKKIIENDMEDLKSSILQSQDIQNAIVSGADAKMINKTLIPRVIIDRYPDLT
                     NEELETVRQHTVADINIAAGRTTTSEDGSREIIKMADRFINIDELNIDLIDQVNPFQR
                     AYEVISRDIDAPTLRFIQDYLSSNKYDFTDEQLVAAYQAAKKFRAENGRNPDRNSKDD
                     GEKYLAFALLRLAEIKREREAAKEDGE"
     gene            complement(50486..53203)
                     /locus_tag="PWU81_000206"
     CDS             complement(50486..53203)
                     /locus_tag="PWU81_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HDL1108945.1"
                     /translation="MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITR
                     AKSKFDNGEPFVIKNKLHYEEIEGNVVIAIDAIAQSIKEQKSKPRYILVNDYLDIAAI
                     DTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDN
                     PDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRTAEDGSNFNEVIKELFAIL
                     DINELARNGKDEWLLEFPYVNGKLFGEPHVDLTFSKVSRELLIEAGELLNWNEINPDI
                     LGAMIQSVASAEDRHVAGMHYTSVPNIMKVIKPLFLDDLTESFETLKLRSEENELKDI
                     TEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLG
                     DLQNNDSMPLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELL
                     PLKDAGNIVCANSLRIDWNEVLRLKENHEIYIMGNPPYLGKSLQNAEQREDMEKLVGD
                     IRKYKLLDYIFGWFWKAKQYIATSHGSFAFVTTNSICQGEQVSILWPYLLKDEVRISF
                     AYQSFKWNNNAKNNAGVTVVIIGMTNNVNIHPKLYLENGSVMSVSNINAYLANGENVF
                     VNESRNSISNLPSLKFGSMANDGGNLVFSVAEYWNLIQKYPDIKHLFKKYLGSTGLIN
                     GDLRYTLWLTEEDATRYKQNSLIKETIKNVKSYREKSKRKETRALAEKPWSFGEVRYD
                     ETSENSILIPRVSSEKRDYVPMGIVGSDTIISDSAMAIYNAPMWLLGLLESRMHMVWL
                     RSVGGKLETRYRYSAGLVYNTFPIQDLSAQRKNEMTRVMTEILDLREYEGGTLADLYN
                     KDSMPESLRKKHQELDGIVDRAYQQRPFESDEDRLGTLLKLYQEMTIND"
     gene            complement(53204..53425)
                     /locus_tag="PWU81_000207"
     CDS             complement(53204..53425)
                     /locus_tag="PWU81_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1108946.1"
                     /translation="MSISYNKLWKLLIDLNMNKTQLREISGVSPNVIAKLGKNELVAM
                     ESLLKIAAALNVDVSEIISSDVIDAEDVK"
     gene            complement(53574..54410)
                     /locus_tag="PWU81_000208"
     CDS             complement(53574..54410)
                     /locus_tag="PWU81_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL1108947.1"
                     /translation="MKQIFIFSDTQKDNISYFEREPAILVSMSRSDLPYLKEQDFSNY
                     PAVYVLIGGNKRYVGQAAGQSISLRLSQHFLKEYKAWVESVLFFTRADGKMSKADTDY
                     LEKRLIQDFQEKSDYEMMNSTAGNTSYIDKLQKAKSDQLYDTVFEIIDEIANIDLFGT
                     SEDSETQIYEFSSSGQYEIEYDGKKISSKSARGLLVDFVSQLFADGNYNNQLQELIVD
                     ETPSFAIILGRKPSMYNGRPNSARVADGVWVYANFNKKNVRIKIDKLARQLGIPVKIK
                     WN"
     gene            complement(54619..55995)
                     /gene="rlmD"
                     /locus_tag="PWU81_000209"
     CDS             complement(54619..55995)
                     /gene="rlmD"
                     /locus_tag="PWU81_000209"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL1108948.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIECV
                     GLLTRVER"
     gene            complement(56106..57161)
                     /locus_tag="PWU81_000210"
     CDS             complement(56106..57161)
                     /locus_tag="PWU81_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL1108949.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(57171..58601)
                     /gene="gatB"
                     /locus_tag="PWU81_000211"
     CDS             complement(57171..58601)
                     /gene="gatB"
                     /locus_tag="PWU81_000211"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDL1108950.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(58598..60070)
                     /gene="gatA"
                     /locus_tag="PWU81_000212"
     CDS             complement(58598..60070)
                     /gene="gatA"
                     /locus_tag="PWU81_000212"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDL1108951.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(60070..60375)
                     /gene="gatC"
                     /locus_tag="PWU81_000213"
     CDS             complement(60070..60375)
                     /gene="gatC"
                     /locus_tag="PWU81_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDL1108952.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(60481..62517)
                     /gene="ligA"
                     /locus_tag="PWU81_000214"
     CDS             complement(60481..62517)
                     /gene="ligA"
                     /locus_tag="PWU81_000214"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL1108953.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(62534..64768)
                     /gene="pcrA"
                     /locus_tag="PWU81_000215"
     CDS             complement(62534..64768)
                     /gene="pcrA"
                     /locus_tag="PWU81_000215"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDL1108954.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            64923..66482
                     /locus_tag="PWU81_000216"
     CDS             64923..66482
                     /locus_tag="PWU81_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HDL1108955.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(66593..68524)
                     /locus_tag="PWU81_000217"
     CDS             complement(66593..68524)
                     /locus_tag="PWU81_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1108956.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(68542..69456)
                     /gene="pfkB"
                     /locus_tag="PWU81_000218"
     CDS             complement(68542..69456)
                     /gene="pfkB"
                     /locus_tag="PWU81_000218"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL1108957.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(69453..70205)
                     /locus_tag="PWU81_000219"
     CDS             complement(69453..70205)
                     /locus_tag="PWU81_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL1108958.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(70364..70573)
                     /locus_tag="PWU81_000220"
     CDS             complement(70364..70573)
                     /locus_tag="PWU81_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108959.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(70584..71912)
                     /locus_tag="PWU81_000221"
     CDS             complement(70584..71912)
                     /locus_tag="PWU81_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108960.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            72048..72203
                     /locus_tag="PWU81_000222"
     CDS             72048..72203
                     /locus_tag="PWU81_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108961.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(72277..72864)
                     /locus_tag="PWU81_000223"
     CDS             complement(72277..72864)
                     /locus_tag="PWU81_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL1108962.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(72947..74197)
                     /gene="clpX"
                     /locus_tag="PWU81_000224"
     CDS             complement(72947..74197)
                     /gene="clpX"
                     /locus_tag="PWU81_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDL1108963.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(74444..75733)
                     /locus_tag="PWU81_000225"
     CDS             complement(74444..75733)
                     /locus_tag="PWU81_000225"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL1108964.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(75887..76819)
                     /locus_tag="PWU81_000226"
     CDS             complement(75887..76819)
                     /locus_tag="PWU81_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108965.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(76976..77635)
                     /locus_tag="PWU81_000227"
     CDS             complement(76976..77635)
                     /locus_tag="PWU81_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL1108966.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(77638..78663)
                     /gene="sppA"
                     /locus_tag="PWU81_000228"
     CDS             complement(77638..78663)
                     /gene="sppA"
                     /locus_tag="PWU81_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL1108967.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(78676..78966)
                     /locus_tag="PWU81_000229"
     CDS             complement(78676..78966)
                     /locus_tag="PWU81_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108968.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            79376..81181
                     /gene="glmS"
                     /locus_tag="PWU81_000230"
     CDS             79376..81181
                     /gene="glmS"
                     /locus_tag="PWU81_000230"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL1108969.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(81253..82608)
                     /locus_tag="PWU81_000231"
     CDS             complement(81253..82608)
                     /locus_tag="PWU81_000231"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL1108970.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(82632..83801)
                     /locus_tag="PWU81_000232"
     CDS             complement(82632..83801)
                     /locus_tag="PWU81_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108971.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(83798..84685)
                     /locus_tag="PWU81_000233"
     CDS             complement(83798..84685)
                     /locus_tag="PWU81_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDL1108972.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(84874..88554)
                     /gene="nifJ"
                     /locus_tag="PWU81_000234"
     CDS             complement(84874..88554)
                     /gene="nifJ"
                     /locus_tag="PWU81_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDL1108973.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            88752..89273
                     /locus_tag="PWU81_000235"
     CDS             88752..89273
                     /locus_tag="PWU81_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL1108974.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(89972..90537)
                     /locus_tag="PWU81_000236"
                     /pseudo
     CDS             complement(89972..90537)
                     /locus_tag="PWU81_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(90716..91372)
                     /locus_tag="PWU81_000237"
     CDS             complement(90716..91372)
                     /locus_tag="PWU81_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108975.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(91940..92875)
                     /gene="whiA"
                     /locus_tag="PWU81_000238"
     CDS             complement(91940..92875)
                     /gene="whiA"
                     /locus_tag="PWU81_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDL1108976.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(92909..93907)
                     /locus_tag="PWU81_000239"
     CDS             complement(92909..93907)
                     /locus_tag="PWU81_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDL1108977.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(93904..94788)
                     /gene="rapZ"
                     /locus_tag="PWU81_000240"
     CDS             complement(93904..94788)
                     /gene="rapZ"
                     /locus_tag="PWU81_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL1108978.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(94923..95654)
                     /locus_tag="PWU81_000241"
     CDS             complement(94923..95654)
                     /locus_tag="PWU81_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HDL1108979.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(95658..96923)
                     /locus_tag="PWU81_000242"
     CDS             complement(95658..96923)
                     /locus_tag="PWU81_000242"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HDL1108980.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(97186..100002)
                     /gene="uvrA"
                     /locus_tag="PWU81_000243"
     CDS             complement(97186..100002)
                     /gene="uvrA"
                     /locus_tag="PWU81_000243"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL1108981.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(100012..102006)
                     /gene="uvrB"
                     /locus_tag="PWU81_000244"
     CDS             complement(100012..102006)
                     /gene="uvrB"
                     /locus_tag="PWU81_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDL1108982.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            102265..103767
                     /locus_tag="PWU81_000245"
     CDS             102265..103767
                     /locus_tag="PWU81_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDL1108983.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            103769..104506
                     /locus_tag="PWU81_000246"
     CDS             103769..104506
                     /locus_tag="PWU81_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL1108984.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
BASE COUNT        29036 a        23225 c        18478 g        34025 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgcg ttgagagtac
      241 actctgtatc ttcctactat aataatggtt gtttatgatt tttgagttga tagattgaat
      301 aggtttgtgg aaacaaatca ctcaaccgtc acttctcttt tgcttaaatt tgaaaagata
      361 gctacttgcc tgtttcaatc catcaagttc atcaaattta tgacgactta tctctttggt
      421 atcacgattg actaaaacga cctcgtaaga gtcagggtta tctaacatag aattatattc
      481 attaatcgac atttcaaatt ctgtaattga tgattttacc tcaaagtaca tattcccatt
      541 ctctgggtca tgaacccaca tatcacaaac aattccgttt ggtggctgct tattacgatc
      601 tgaagattta ctagaatatt tcgtattcca aattacatta agtttttgat tctgagtcaa
      661 ttcagcgtaa gcaatttgtt ctgctgtttc accagcatca tcatttgcgg agtttctatt
      721 atcataatct cgtttacttc tttctccatt tcgtgtgttc ttacctttcc tactaacact
      781 attcgatggt tcaagaatgg tttgaacagt tgatgttcct tcaatagaga taccagattt
      841 ttctccgttc aaatcaacat cattttcttt agtaacgaat atcagtatat cttctcttaa
      901 acgggacgga ataccaaaat acagtaagga agaaactttt tgatgacctc taatataata
      961 atcaaaatca tctattgtaa tagctgcaac cgtgactaac tcttcgacat tattatcata
     1021 caggccatca atgtcaattt catcagtaat tgacacactt agctctggaa atctcttctt
     1081 actttctttc tcaatgtcga cttgaactga atttggaatt tcagaaatct caaattgata
     1141 atctttgtat ctatcaattt tttctaggaa tgtttctttg agtatttcat caccgctttt
     1201 atttgcattg ctatataaaa tctggcggta atgttccgct ttcttatcta gataagatct
     1261 taatttcagt ttccaaaatt cacgaatatc caaatgaatc aattctgaca atccattcaa
     1321 atcctcaata tcttttgaaa tgctttctag agctatcgcc aacaattgat actcttcttc
     1381 ttttaatgca tatgaatagt caatttcaaa aatggtagga tattcctctt catttagtcc
     1441 atttgataac aaaaagtcat taactttttt atttaacgca ttttcgctaa acaatagctc
     1501 aaacgattca ttccacttat ctacaccgaa ttcttcagtt atttttcttt gtttatgttc
     1561 cgtatctcgg gaaattaaca attcaatcaa attcaaatct aagcttagat ttaacacctg
     1621 cttgaatatg tctacgattg ttgaagaaaa cgtatcaatc aaacgtaatt gattaatttc
     1681 aatagaatta gcaggtaatt ttaagtagaa ttttccttta ctttccacaa aataataatc
     1741 ttccaaaacg attgactgac catcttgctc aacatcttta gcagatattt tgctgcatgg
     1801 aataatctga atacgtttta atgaactaac attcttacta ttgtcatcta tcgtagataa
     1861 taccgcagtt ttgatatcat caatctcttt tttgaaatcc tcagaataat cttccaataa
     1921 ctcaaatgtt tccaatttta aatttgtttt gaagcgttca accccaagcc attcatatat
     1981 cgtagttgta ctagcttttt caggcacgta aataaaacgc atttgttttt ctattgattt
     2041 aggcagtcgc ttatcagcat accttaattc tttgtttgga taaaagaagc catctttggc
     2101 taagactgat aatccttcgg tagaataagt aggattcatt ccttttttat ttcttataag
     2161 ttcattatat aatctgcgac ttatttcgcc tttatcgaat tttggtaact caactaatat
     2221 agctcttatt tcatcatccg aaaacatcgc catatttttt ctgaaatcca actgttcaac
     2281 aatgcgttgc cccaagagtt ccattaagct ttgttcagaa actccataca atcccggaac
     2341 gtgtgcctgg agcttttcat atagcacaat ttgtctcggg ctatattttt ctccatcaat
     2401 cttaatccat gcagttttat tcaaaatgta tttcacatac tcatttgaat taaagtacaa
     2461 tccactccag ttcgattgct gacttgcact tgattctttt tttaccgaag ataataaatc
     2521 tgtcaattct tcgtcttgat tgagccaatt gacaatgtct ttcgtatcta atttgtctat
     2581 taattcttca aaattactga tgtacttaga agttaaataa ttaatattcg taaattttat
     2641 ttcattctta tatttgttat aaataaactt tttaaatcca acatcatttt ttaaagaatc
     2701 gttatcataa atcttaggat atttggaaac accacatcga agaataaatg acagaaaatc
     2761 tctctcctca gcaaaactag agcaatctat ttttttcaat gccgtgtaat ctgtatcttg
     2821 aaaaagctta tctcctaatt cattgccata ctcttttcca atatacattt tattggaagg
     2881 ttgaacgtca ccagcaacgt ttggaagatt aaatggaact ttagacagct cacttccagc
     2941 tgatagtttt tcattgtagt tatcataaaa ccaattaatg aaactgatag atttttcaac
     3001 agtgtcaatt tgtcgattaa tcgttccaat gatcaaatct gaactgcttt gctcagtgaa
     3061 ttcaattgca agatattttt cactgaatgc atctagaata cgtttatctc ctgtacgttc
     3121 agctttgtat gcattagctg tttcttccca tttatttttc caacttgatg aagtcttaat
     3181 ctgataaatt aactcatcaa catatgtttg tttcaaaatg cttaaactaa cccaagaaga
     3241 cagctctttt ggaagcacag taacacctgt atcggtcggt aagaatacct tatctgattt
     3301 catcaaccaa ttgtcactat tgtcctttaa tagcttaggg acaatcggag aacttttata
     3361 ttgctcactc caccataaga acacttttgt ccttattcta ttttcccaag aagatgaaag
     3421 aatgttgatt ttatcagata gttcatctac aacaaacgac aggccagtgt attcattata
     3481 attagcaagt tcttgtacta gttggtcatt ttcgtttgtt tctaataatt gcaacaaggt
     3541 ggaaaatgtc tcacctatta gctcctcagg gaattcttga aggaaaagtt ttggattatc
     3601 tttgatagaa atatattgcc aattgacagt cggtagtatt tgggcattag ataaaatacc
     3661 caagaattcg ttcattaagt caaatacatc taagtcccat agtttattct ctttaaaagg
     3721 agttaccatt tttaatgcta tgtcttcata ttcaggctta gataattttt ctgctacttc
     3781 agaaataaat ttgagcacct caataaatat tttcttattt atttgttcac tatcatccgg
     3841 gacgtcatca cgtgaattat ttgttataaa cggtgcattt ataagtgcat gaattggtgt
     3901 accaaaatta cgtactggaa agtagcaata tagtttttct ttggagtaat cgtaatcaga
     3961 atctttagaa acggctagtg caattttagc aactttatct cctatcatag attctttgtc
     4021 aaaaacaaaa tattcttcag acggtgattg accagtaaca tcactttttt ctatcaatac
     4081 atcatttcca tccacaattt tctctgatat tttttcatca tcagttgttt caataacaat
     4141 ttttgttata tttggtaaaa ataacagtga cttataaaat ggctgctgaa gttgtttaga
     4201 aacactcgtg cctttactta aattagtttc ttttagtttt actcgaatta atgtatcaaa
     4261 accagaatca attttgttta ttggttgagg acagttcatt attggaaaac ataaatcata
     4321 atcttctttt ttccaatttt tcttctgctc aaaaattaat tcactgaatt tgtcagaatc
     4381 tcttgataca tactgctcaa aaagcttttt tgtgaattct tcactaaatc gaatagcaaa
     4441 ttctcccgaa tacagttcga cccactcagc atcgtttagc aaggaacgaa aaccagtccc
     4501 cttatttccg atattttgtg atgatttatt actcgctctt cctaagcaca atctttcaat
     4561 tgtatctttt gaaaatgaag tgcctgtatt cccaacttcc aagatattat ttttatatgt
     4621 tatctgaact ttcacttcgt cattgctagc atcattttct gattttatat ctccataggc
     4681 atcgtctaca ttctgtaata actctaaaat ctgacgccca ttataatcac gcatcaattc
     4741 atattcttcg ctgcaggcct tttcaagttc attactcaac tcagaatccg tatatttacg
     4801 aataaaaata tcccttaatt catcaatggt attccattgg ctcacagaac ttcgccccca
     4861 gcctattttt tacataatat attatatcat caatcacttt atgaaatcca atcaataaag
     4921 acgttaatat ggctacccac attaacgtct agtcctatct attcaaatta aatttctaca
     4981 gtcactctca ccccactttt gaaatcaaca acaatgttct tttcaaaaat ggtgaccttc
     5041 tcaatcaacc ttcgaacaag cacttcatta tactcattga tgacatcgtg ttcttttaca
     5101 aagactacca tctcatcaac ccgttgttgc aaattagtat tcgctgctat ttgttttgca
     5161 accgcatctc gttcatctct tagtgaaaat atttggcttg ccaattcatc gtaaccctga
     5221 ccgccaactg ccaggctaat aatttcagct tcaagctctt tcaactgact gtctagttct
     5281 gccaatcggt cagttaatcc atcagtcata actgagttga tattgtcatt taattgctga
     5341 agaaaagcat cacgatcgat taacgtttca ttgattgcct tgataacagc tccatgcagg
     5401 tctgtttcgt agattgttcg acccgtgcaa ttatgttctc gtttatccac tctggatttg
     5461 catcgccaca cagttcctcg attcttccaa tgaactctgc ggtaagcatt gccacagtca
     5521 ccgcaaactg tgataccaga taacgcatac ttcccagaaa agcactgtga ctttttgttt
     5581 ttcattgatt tacgccggtc tagttcttgc tgggcttgca tgaagatatc ttttgggata
     5641 atggccggat ggctatcttc aacataatac tgctgagcgt aactcccgtc attcttcact
     5701 cgtttctttg tcaagaaatc ggtagtatat gttttttgaa gcagcgcatc ccccatgtac
     5761 ttctcgtttc gaagaatcaa tctgactcct tctcctcgcc agagctttcg cttccctccc
     5821 gttaggatgc catcagccat tagaccatta gcaatttctt gcaaggaact gccctcaagg
     5881 tattcgcgga agatgcgttt gatgacttct gcttcctttg gttcaatcac caagttacca
     5941 tcttcgtcct tagtgtaccc aagaaaatga ttgtggttga tacgtagctg tccctgctgg
     6001 aaacgatact gtacgcccat cttggtgttt tgacttagtg actggctttc ttgctgggcc
     6061 agacttgcca taatggtcaa aagtacctca cccttcgcat ccattgtatt gatgttttcc
     6121 ttttcaaaaa acactgcaat cttcagttct tttaactgtc gtatgtattg caagcaatca
     6181 accgtattac gggcaaagcg tgaaattgat tttgtgatga tgtactcaac ttcgccgttt
     6241 tgacacgcct caatcatccg gttaaaaccg tcacgctttt tcgtcccagt tcctgaaatc
     6301 ccttcatccg caaatattcc tacaagctcc caatcgggat ttctttgaat gtagtccgta
     6361 tagtgttgaa cctgtgcttc gtaagaactc gcctgttcct ccatttcagt agaaacacga
     6421 caataggctg ccactttcaa ctttggtttc tcttcggtat tctctgctgc ccattgtcta
     6481 ggcttcattg gaataaccgt cacctgtttt cctgccattt cacttaacct ccttgatttt
     6541 tgaatatgca tattgcgctt gtttgaatgg gtcagaatac ttttcggtaa tcttgccaga
     6601 tttaaaatga gtgggtatta ccttcgtatc tggcactttg atctccttgc gttttttaaa
     6661 gaacttaatt cttcgttctt gctccagatc cgcttggaag aaaatatcct catcaatgat
     6721 ttttggatat atctcatttc ctaagtaaac ctcattcatc aaaatacgtt tcataaaggg
     6781 atgcggcttg ttaatgccag agcgtttgcc agcttcttgt aagccaacac cctcaaggta
     6841 aatattgaag aactcacgta tttgttgagc ttgctgttca tccacgttga tttctccatt
     6901 cttaatgaca tacccgtatg gaattgtatt gttcattctg gtaacctctc tttcaaaagt
     6961 agtccgcact taagttcaaa tccgacttct tggcggttgt aaactgtaat gctgtttgtg
     7021 aacttcttga ataaatcttc atcccagtcc gtcaacatct ctgccatccg cgcgaatttg
     7081 tataaggctt ctagttcaat ttgctgagtg cttttgccgt tgactgactc aatgatgagg
     7141 cgctgctttt cttgtgcgac agccctctct tgttgaagtt tgctttgttc ttctaaagac
     7201 accttcgttg ataaaatact ggaagcagtc aaacttgcca gaacctcaag cttttcttga
     7261 atcttgtcga tttcatcagc aagcacttcg agcccttcct gattcgcacc tgctttattc
     7321 aaagacttca taaaaggttc cagaaccaac ttgtgtccga aaatcaactt attcaacatg
     7381 gtcaaataag caaccttcaa atcttcatcc gtaattggta atgcatcgca gctatcttta
     7441 tttttaagat gccccgtaca cttccactga accttcttcg tattaccttt ccaataagtg
     7501 tgaacccgct tgaatttgct gccgcattta tcacagatga tgttgcctga aaatgcgtat
     7561 cgcttggtgt actttttccc gtcaccagta ttccctttgg cctcagcttt tctccgaatg
     7621 agttcttgaa ctcggtcgta atcttcttta gatactatgg gttcgtgatt gtttttgatc
     7681 tggtacattg gcaattcgcc cttgtttatg cgtcgtttgt actggtcatc aacaaaggtt
     7741 tgttgcatcg tcaaagtccc ggtataaact tcatttttca aaatacttct gactgatgtt
     7801 gcttgccaat ttgccccacg tttagtgaat tgttttccat cgtttaaccg cttggcaatc
     7861 attccagtcg aaatgccatc taatgcccac tggaacattt gtttaacaac ttttgcttca
     7921 gctgcaacga tgtgaaaatc atagcctttc cgcttgtagc catacggcgg gtaagctttg
     7981 aatttccctt tagacatccg gtttcgaatc gcccacttca agttttctga atgggaatag
     8041 gattcacttt ctgccaagct tgataaaatt gaaagcaaca actcgctttc catatcacca
     8101 gtattaatgt tttccttttc aaaaaagatg gtgacaccaa tatcagttag ctgccgaacc
     8161 agattcaagc agtcaactgt atttcttgaa aatctggaaa tagactttgt cagaatcaaa
     8221 tcaatctttc caagctctgc atccttaatc aatcgtttta aaccatcacg ttttacagtc
     8281 ttggttccag aaatcccttc atcataataa acaccgacat agtcccattg ttcatggttc
     8341 ttgatgtgat tttcatagtg agtaatctgg gtttgtaaac ttgccagctg ttcattactt
     8401 gatgttgata cacgagcgta tgctgccacc tttagtttgg aagcttccgg caactctgga
     8461 cgtgccaatt tatgtatcgt tttcatcagc tcaactcctt tccattaggt gacatgttag
     8521 ctctaaagtg ccgttatatc aacgtttcag accattatcc ttgataaaaa aggattgaat
     8581 gtttcccggt ttttattgtc gattttgacg tattcttcca ggttgatttt accggtaaca
     8641 agtaagttca gaaggatctg ctgtgaaatg taataggctg cttcatctga atagttaatt
     8701 tctgttttct tgttagtcat tgctttcgcc tcctcaagat acaggcgaag aaaatccatg
     8761 aaagtaaacc caatcacaaa ttttcctttg cctttcatcc atatgcgaaa gaaataaccg
     8821 actcgaaccc acaaaacgaa aaaagcctgc aagaatgaaa cgaatcactc ttgcaggctt
     8881 ctaagtttgt ttatatatag taggaattat tctttgtaaa ttttgacttt ttacttccca
     8941 aacatcaaat aagcctttac aagcttcatc caataaataa caatataaat atcaattgtg
     9001 ggaaggagaa agttatgagt agttttgata tggaagggtt agaaaaagaa tatgaacatt
     9061 tttgcaaatc tgcgacaaca aaggaactgg aggaatttga tgaaagcttc aaacaagaat
     9121 ttgaaaagtg ctttaaaacc atagaggctg aagaagcttt ataccagttt ttacaagcca
     9181 atataaacgc gatgaataat cgtatggaat caatagaatc gcctcttttc tgcatcaatt
     9241 acaaagagca ggtatttcag ttaagaaatc atttaatcct aatgcaaaat gctgtaaaca
     9301 cctaccttaa agaaaactag acgaaaaaaa ggccccacaa taaaacttgt gaggctgttt
     9361 tgaattataa agacgtcaaa ctacttattg aaaattttgt agctcgttgg aaccatcgtg
     9421 aagttttctt cagtaattgg gccatcataa attttgattt tgcaaacttt tgacgccaca
     9481 tcaaggtcgt gaacaaagta aattttatat ggtgtatctc caagggaggc agcaacctca
     9541 ttagtactca tttcaaatcc gtcagagaag tttgtttttg aagctttcac ttcaatatgg
     9601 atttcattac ctgattggtc aaacgcacaa atatcatagc catgaccatc tccttcagtt
     9661 ttagaaacat gttcaggaag ttttgttttg ttcttttctg cttcctgaat gaggaaatca
     9721 agtacgattt cttcgcctag agcacccgtt ttttgttttg ccttgttgac acgctcccaa
     9781 tcagtatggc gtccagtgaa ttttcgtttc tgagtcccat tcgtgcgatt tggtcccact
     9841 tcatcaacaa gaacagtaaa tccttttgaa ctttcttgct caatttcttc aacttcctcg
     9901 tttgtaagat ccagttcaga cggcaacgat tgtgtatcat cgattgggat aagatgacga
     9961 ttttcaatca atgcaacaaa atcggcatag gtaatttcat tcattccata ctgatcccag
    10021 aagtaggccc atgaattcgg ctttttacgc ttctttttgc taggtgtatt ccaatcgaag
    10081 ttttcaataa atgaatcacc ttgtttaatt gccggttcga ttgtaaatgc gtgtgtaatt
    10141 actgccgtaa catttccagc ttcatcaggt tcagaaatgc tctcaacgta tccgcccccg
    10201 taaatttccc acttacggtc tttagtaatt ttccccggat gacgattcaa aacaaccgcg
    10261 ccggctttca atttaccata ttggcgcatg ttccaaccgt aacttttagc atcatcagcg
    10321 tatttcaact gaccatcatc ttctgttttc ccgtgatcta aaatgaaagc ttgttggctt
    10381 tctgttggtt ctggtaacaa atctttaaac gtaaccatat cgatacctct ttctataatt
    10441 caatcggaac aaatttatgt tctggttgaa ttaataataa cacgttcgat attttaagtc
    10501 aacaatccgg acataatttt gttccgatta aatttatgaa atcaaaatat aatcgtcaga
    10561 ttccgtctta ccagtattct taccgacagc acaatatcca atcgggcaac gagcccatac
    10621 ccaaccatcc gcaatcgtca tcttctctga cttcggaagc gtccaagttg cgcctttatt
    10681 gtatgaacca acaaccgcac cacttaaact tggcttgttg cgaacgttga gaccactcac
    10741 ttgaaccaca agagttttca cgttggatgt tgatgaccca gatggataaa cttgattacc
    10801 gttctggtcg aacaccttat aaccagaatg gctatctgca gcccgcttcg cattgttcaa
    10861 gtccttatag gcaccaattt gtgacttggc gtcagcccat gatttgcgaa cacgatacag
    10921 accactagct ggcgtgcttg gggttgatga accattatta atcgcattgg ctttatccgc
    10981 aatctcctgc agtttaccaa gcaactgtcc cggacatgat gtggccgaaa aatgtgagtg
    11041 agggaagata ttcttgttca ccaccaattt ccctagacca taacgtttgg caatatctgc
    11101 acacaacttt gcacttgcat caatagttgc ttgattgata ccccagttcg gtgatccagt
    11161 gctattgcag tgctcaatcc cgatacttga gatattggcg ttccaattac cagcgtgcca
    11221 agctgtgttt ccttcgtcca cgtatgctcg aatttctcca ttttttccaa taccgtagtg
    11281 tgctgacgcc tcacgcgttt tccaaatatc aggaacaata tcatagttgg tggttgccat
    11341 gtgatgaatc actatcttat ctactttggc accactacga ccattcgagt aatggctaaa
    11401 tgactgaaat ccatattttg ttgctagatt actaattccc attttctgtt tcctccttct
    11461 tttcgtgtaa ctgctgcaag atttcacgca acttttccgg cactggcagt ccaatcgcca
    11521 cggcattctc cagcagtgaa attccttcgt ttgagatata gaagaaaatg accgcatcac
    11581 gcagtacatt tccacctttg atgacttcca catcaagcat atgagccaca ccaaccaaaa
    11641 gaaaaatcat gattttctta gtgatgccct ttgccccaat cttgctggat agtttctttt
    11701 cgttgattgc acgaagcaga ccagttacat agtccactac cacaaaagtt acaagcgtat
    11761 aaatcaaacc atcaattcct cccacatacc atccaatcac tcctccacca cctgcgatgg
    11821 cgagttgaat ccaattccac acttctttca tttacaaatc ctccaattgt tctaaaatta
    11881 aatcgagcat ttcagcatct ttccctgaaa tggtgacttc actattttca aatccagcaa
    11941 ccaataaagg cagcttgtct tgaagttcat ccacgttgat gtgggtttct tcgttgagca
    12001 agtctgcctt ttctttgtga tattcagttg cggtttctgg gatgagtgaa aaggcaccgt
    12061 catgttggac caaattgccg tgctcatctt tctttccgta tttttgaagc aagtctacct
    12121 cgtcttgata gagttcatca accttcacct ggatgagttt tgcaagcttc gttcgcactc
    12181 ggctaatctt tggtggtaag tcaaaacctt ttaagaaact catcacgtcc accaaatctt
    12241 catttctaat tttgagcatt atctatcttc tcctttaaat caccaatctg gacacaaaga
    12301 tcatccacga ccatcacaag caaatcaacc atatccgtcc caactttcat tggctttgga
    12361 atatctgcct cgataggttc agttactatc acttcttctt tttcttcgtt catggcggcc
    12421 tcctaactgt atgtggtcat tgaattaaac gactgcggat tgtaccaacc actcgctgta
    12481 ccgttgctac tgaaaccggt tggtaaagcg attgttcgtc ccgcaagttt ggcacagatt
    12541 tcacggatga ctccaaagtc aacccacttc ccatcatctc cgagcatcag attagaaccg
    12601 ttgaatgcaa taccagcgct gccggaactt gagccaatct tagtataaag ggaactacta
    12661 aatgtaatcc caccaacaga tatttgatgt tgtgtcccta tacgaagcat tgatggtaaa
    12721 taaatgtcgt cgtcaaagtg gaaccccttc tccataccag actgcaatgt tctcgcatac
    12781 caagaaagct tctgggtgta gacactcgca gaggaggaat ctttatgagc ccatgacata
    12841 tactctgttc catactcaag atcaaatacc aatccacgcc aatcagagtg accactgatg
    12901 ttatttgcac caattgttcc gactgtcgtt ccaccacgtc gaatggtcat tccctgcgag
    12961 tttatttcag tcacaagagt gcttcctgaa taatatccaa tcgtagttcc tgtgattcga
    13021 atggacgaac tcgcagtcaa tgctgttagg atattagcag caatcttatc agccgtaatc
    13081 gagttcgcgc caatacgggc tgccgacaaa gttcctgtgg taatcttcgc cgcatcaatg
    13141 cttgcaattt ttgcactttg aattgtggca tctgcaatct ttgcgtttgt gatggccgca
    13201 ttttggatca tggcagaact aatcactgca ctatcaatgg ttgtttgacc agtgatgtgc
    13261 accttgtttc cagcaatcaa aatactttca ggtgagatat tgatttgatt aatcacgtct
    13321 cctttaccaa ctcgtaaatt gattaagtct ttctgcatga gaattgctga atataatgtg
    13381 ttggcattgg tatagtcttg ccttgtggtc gtaggataaa tatttccttc atattcatcg
    13441 ggtttgatgc tcgtgttcac tcggactcgc attttcaatg tgccactcgg gactgtaatt
    13501 gctccgctcg aaccagtata agagatttga tgataacttg aaccatccga cgggtttaga
    13561 gtaaaaggtg tgttgcttga attataaatc ccagcatatg tgccagtctt aactgaaaag
    13621 aaagctagat acacgctgtc agtagtaatc cctgatatct gctcagccgt taacgtccaa
    13681 gaataagtgg cattgctgct acttgtaacg gtcgaaatct gttcccaagc accacctgta
    13741 taaagcaaga caatattatc tgatgtatcc ttgcgaccat tccacgaaat tacactcgga
    13801 gcgactttgc cttccaatgg caatttgtaa atgaccagat aatctcctga atttgaatag
    13861 gggtttgttg tcacgccact tgtaatactc atcgattcaa cgttcttact tactagataa
    13921 ctcgcaatgg tcgcattagt ataagttccc gccgttaggg tttcactaaa ttgttttgcc
    13981 gttgggacaa aatcaatgta aggtaaatcc atgccgtagt aatgatagta gaaagcagct
    14041 tgagttccgt cacgcagttg cccgatatac ttatctccca ctttgacatc aaggaactca
    14101 cttcggagat aattggttgc ggacacttct gaaccgtcag tggtactcaa acttccttgt
    14161 tcccattgat tgactttaaa tggaacgccg attgcttctt tggtttctga aacacttaac
    14221 agtaaggcat cagtggtcgc tgtaagtttt acgacgttac ttatctcgga taaatcaaca
    14281 tagccatttg tcacatccgt tttcaacata tagcaactga tggtttctgg tgcatcacta
    14341 gttgttaaac gaatgcgaat aaaagcggca ctgcttggaa cagtaagcgg tgtggtggtg
    14401 tttccaaatt gggtgtacga taaaaaggac ttatcctctt tgtaataaag cagataagcc
    14461 gttgcgtaag tagagtaaag tttcccaccc acaaatgttt gaagtagata cttttcgcca
    14521 tttctaactc ttgtaaaacc tgagcgaacg tatccaccat aggtattttc ttttccatca
    14581 tttgtattca gaccaccaac tgcccactgc aaatctgcaa tgctcatacc ggctaaaaca
    14641 ttgaccgctt tatttgtttt agaaagagat agctcaaatc tatcagcatt ttgggtgatg
    14701 gtgctttctg cagttgtaat acgccctgtc agattatcaa ccgtcgattg acttgcctta
    14761 ctagcaatct gcccagcttg cactgtcaga gttgattcag ctgtgattac tcgtccttca
    14821 agagttgaca taccactcgc cactaatgaa atcttgctag tattctgttc aacggatgta
    14881 gtaagcgttt caacacttgc acctacactt tcagctgcat tcaaagcatt cacagcatcg
    14941 ttaaatgctt gattggcctt ttcagtggca gtattggcat ccttttgtgc ttgttccacg
    15001 gcttgcttta cttcttctgc cgtcatatct gtcataagtg gataccactg agtgacgcca
    15061 tcacggattt catacaccca catttcaaac ttgtcaccat tttctttgta ccaaacgtca
    15121 ccgagtttcg gattatttgg agttgtcggg ccatagaagt tggtgttttt accatttgcc
    15181 gaactcaatg cgtaattggc atcgtctttg gcttgctccg ccttgttgtc cacccggttg
    15241 atgtccttcg ccacatccgt gaattttggc gtgatgtttc cgagtgttac tgagatatac
    15301 gctttcagaa atggatcgta tttgtatgag accattctag ctgtgatgtt caatccatct
    15361 tcggcatgaa taactgaaac agtatcaccc aaattaatcg tttcaagctg tgcaaaatct
    15421 ttatattctt cagttcgctc aagcggggca aactccacat cataggtggc ttttggcaaa
    15481 tcgaccttgt tagctgagta ttctaaccca gccaatctgc gaagttcatc ataggcctcg
    15541 gcttgagtaa actcatcctc ttcatcacca actttgacgt tgtcgtactt gatgactttg
    15601 attttaggct ggacgtattg gttgatgttt ggactgtcca cgtatttttc gggaagaaat
    15661 agtccatcat acccttgtgg cataatgcga gtaacaattc cagagtaatc aacatcagct
    15721 ttatatcctg tcagattctt cttgtctcgg atttgtacgc cgttgttact gccacgagtc
    15781 gttaacattg aaatcgtgaa gttatcgcgt acaatttcac caccataact gctttgaaaa
    15841 ccattctcta ggtcactatc aagcaacacc tctacaggat ttagtcgcac caagcggaca
    15901 ttattgaccg cactgatgtt tgatgtaccg gtaaaacggt gcatgaattg agttgcacca
    15961 agcagttgtg tcaaagctcc cgacccgttc ttgttgacga tataagtatc ttcaataaag
    16021 tttgtggcta ggtcatagaa aatatgatga gctactatgt gcaaaaaacc accaagtgca
    16081 tcatctcgtt caacaatcct gaaaagctgg tcggacagtt ctggtactgg tgctttcaca
    16141 atcatttctg gcaacaattc ctcactgtgt ggagcatgaa ttggataatc gaattcaaag
    16201 gaaaagagtc cgttcaattc ctcactcacc acggggttca gaatgttgcc atcaaggaca
    16261 gcgagaccgt tatgattgaa atttgattct gttttgttgt aaagcgtaat cataagtatc
    16321 gccacctcgg ttctatttct actttggtta tacctgtacc aagagtcaca gtattactgc
    16381 cgactcgaaa ctcaggaaag tcacctgtca tgtattgatt ggctgccaca tccccgtaaa
    16441 agcattcttt caaatccgaa tctagtgtca ggttgccatt cgtcagattt aacacgatag
    16501 gcttgccatt tatcgtgagt gtcctagttc cattgccgta gaccttaatc ttcggcaatg
    16561 agtaaatgtt gccgggatta ttgaccgttc ctgacgtggt tcgattgata gtcgctacat
    16621 tggtcttata acgaaaaggc gaacaggtta actccatttc aaactcccac atggaagata
    16681 aaatctgagt tagtccgctc gccttagcat acttgattgg gtaaaacacg tttggttcat
    16741 ttgaaaatgc gatagtttta gcgtttagga tttgtggcaa aattatctgc cagctagtcc
    16801 aaacatcaga tttccaaatc actaccttaa aaccgaaagt cacatctttc cagcctttca
    16861 gaatggtcaa atctccttca cgtccatcaa tctcaatact atcaatgaca cgttccgtca
    16921 ctggaataac tggcacttcc gtaattcgca aacctaaatc agtccgtgaa ttaattgttc
    16981 cgtctaaaat aaatgcgttc aattgaccac cgcccatcta aataactaaa aaaggaccag
    17041 acaacaatgt ctaatcctaa agtttataat ctatcatcct attcggttgt ttcttccagc
    17101 cctattttct tggtaatcgc taaatacgcg tatacaacca gcacgccgga aattccatat
    17161 attgcatcga ttagatctgg tgtattcaga aaagtcatca tcgtttcaca tacaatattt
    17221 gcaacaatca gaccagctcc catcgcagta agaaccctta aattcgttcc cataagcaac
    17281 caaagatagc ctatcaccaa atagaaaaga aagcttaatg cgaagtttga aatgtgagaa
    17341 ttaatctgat tgttgatgaa tacaaaacca tgatttatgc cgctatacca tcttactgcc
    17401 gttaaaaata atccaatagc aagcagtaga atatatccta tcagtgcagg tttctttgct
    17461 ttaaatttat gcttcaacat atgattacca ccatctcttc agtaaacact cgttagttat
    17521 agtatacagt caaaatgtga aaatccaaca ctaatatgct acacgtctac gattcaacgt
    17581 cgccaagctc ttatcaattt ctggagcaag tttgccgacc aatgttccat cattcaagac
    17641 gactttaatg tccattgctt taagtagcgt tggcaacatt tcactcgtga tgtttgttaa
    17701 ggcatcaacc ttagcaccta aatcaagcat cgagaatgtg ctatcaacgg cagcgtggat
    17761 atcggtatca atatcaaagc ttggcatata aagttttgtt ggaatagctt tctgcatcgt
    17821 tttagaaaca ccagtcattt ccttatcaaa acctacgcca atcccttgtg ccatgtactt
    17881 cccgatttca tcacgaaaca gtgttgatgg tgaatgaata ccaaagaagt ccttgatgtt
    17941 gtctactaca ccgccaaaga atcctgaaat cttgttgcgt aaccaacttc cagcatcagc
    18001 gataccattc cacaatcctg taatcaagtt cttcccgata gttcccatag ttgaaacata
    18061 ctcaccgaaa gcattcgcaa gccctgtgat gatttgcggt actgctttga caatggcaga
    18121 aatgattgct ggtaagtttt gaactaatgc cacaagcaac ttcacaccag cattgatgat
    18181 tttgtcgatg ttgtttacca aagttcctgt tattccgttg ataatttgcg gcatggcatt
    18241 cgcaatcgtt gtgataattt gcggtaacgc accaatcaat gccacaagta actgaatacc
    18301 tgcgtcaatc aactgaggga ttgccccaat cacggcattg atgatatttt gaataatcac
    18361 tggaatagct gcaacaatcg cattgatgat atccggcaaa gctgtcacaa gtgatgtcaa
    18421 aagttgaata ccagtctgaa tgatttgtgg aatagcactg ataataaagt tcacgattcc
    18481 tgtaataatc tgtggcagac tttcaatcag tattggaagt gctgtgataa gtccttgggc
    18541 aagtcccatg ataagttgta aagcagcatc caacagtaat ggcaagtttt caatcaagcc
    18601 gttcacaatc gttatgattg cctgtactgt aattggaata agttctggca gcgcttcgcc
    18661 tagtccttgg ataagtgttg tcacaagttg aaaggcagcg tcaatcagta aaggtaagtt
    18721 ctcaataagt gtttgaacaa tcgttaaaac ggcttgaacc accactggaa taagtgtggg
    18781 aagtaagcca agtatcgttt caagcacttg tgagaataat gaggttactg cttctaagag
    18841 tgttggcaat aactgcccca atgcttgaat aatggcatcc accactgccg gtaaagccga
    18901 cacgatattt tgaatgaccg gtagaatatt cttgatgacg ttttgaaatt gttccaccac
    18961 attgccaatc aacataccaa tgtcagcgct gctgttacca agtccagcca tcaagtttga
    19021 gattgcagac ttcataccgt caatcgaacc acttatcgtc tcactcgctt ccttggcggt
    19081 tgtaccagtg atgcccattt cggtttgaat aacgtgaatc gcttcagtca catcagcgaa
    19141 gttcgagata tcatatttga caccagaaat cttctcagcc tcttggagca aacgttccat
    19201 ttcggacttc gtgcccccaa aacctaattt caagttgtcc agcatcccgt aattttgctt
    19261 ggcgaaacct tgataggcgt tctggatgga ctcaatatcc gtccccatct tgtttgcatt
    19321 atcgctcata tcagtaacag cttgatccgc aactcctgcg gctttcttgg tatcaccacc
    19381 gagagatgaa atcaaggcag ctgagaaacc tgtgacagtt tccatgtatt cattggctga
    19441 cattccggca gttttgaaag cattgtcggc attcttttga acggtggatg acgcatcctt
    19501 gaaaagagta tccaccccac cgactaattg ttcatagtcc gcgtaagcag ataccacttg
    19561 ttttcctagc tccacggcag cagcgccagc agcaagtgcc actgtaccca ttgctacccc
    19621 gatacctttt agaacgccac cgaacttttc aaatttactt ccagactttt cagcctcgtc
    19681 acctgtatcc ttgagttcgt tgccaagctc atcagttttc ttagcagcat caacttcttc
    19741 agtacccatg gcgtcaattg ccttctcgtt gttagcaact tcacgctcca tattattgag
    19801 ttgggctttt gcattgttta attgcactgt ccaattctga gtacgcttat cgttttcacc
    19861 aaaactgtca ctagcgttct tcaaagcatt ttcaagggtg gtgatttttt ctttctgggc
    19921 atcaatcgat ttgtttaaga cttggttgcg agcagacagt gagctgatgc ttttatcgtt
    19981 tttatcaaat tcggatgata ccagtttcat ttctgaacct agcaccttga atgattggtt
    20041 gatttcacgc aacgagttct taaactcctt ttcaccttca acgccaatct ttaaaccaaa
    20101 attatctgcc aaataaatca ccaccttccc ttaaattcca ctcggtatca catcatcaat
    20161 gaataactca actttcggct tgctaatccc agtaaattgc ttatgacact cccataaatc
    20221 caaaaacagt ccaagcgagc agtaccagaa atcatctgac gtcataccca tttgaactgt
    20281 cccatagtaa tacaggcgag taaatgtttc ttcatcactt actcgcctgc tttgtttttt
    20341 gaatctgttt cactttcgat attgcgagct gtgcccttga acattgcttc agtgattgct
    20401 gatttatatt cagccaactg caacggactc gtcatcaact ccacatattc agtcgtgagt
    20461 tcatctttct tttctgtgtt cttcaagttg taaatcttga tggactgatt cgccaacagc
    20521 gtaatcaacc agatgatttc atcaagcgcc aattcaaagt tctcggattt caacaatttc
    20581 tcaccaaggt tatcaagccc accgtagtga ctggcgattt ctttcgtggc ttttgttgtc
    20641 aaaatcagtt catattcatc tccgccaagg ctaatcttgg cactgcgttc ttcactcatt
    20701 gttgcctcct agtctgctgt gaaatctggt tcataaacac tcgtgtacca tccattgata
    20761 gttgtttgtt caactcccgt atcgttttca tcaacttctg ctttccatgg atgcttacca
    20821 ctagcatcta acttgttacg acgcatgact gtaccctcaa ttgttggtgt tgagaatgta
    20881 atgtcgtcac cttttgttgc taggcttgtg ctgggcacgg caaacttcac tcggtacaac
    20941 cacaaatatt tgtatttgcc attcgattta cgagcacgga aaccaatcgc aactggtgtg
    21001 ccaccatctt cggttgtcga aattaagaca ccgttttgat ccacggttgc accaacaagc
    21061 actgccgcca cagcacgtcc aatatcatca acaccgagcg ttaactttcc atttttaaat
    21121 tctttcacta cttctgatgc accatcatcg gcgtataaag ttgcttcagc aagttccacc
    21181 gaaagctcag ctgagattgc tttcgcaagc atagcgggtg tgccgtatgt ttcttcgcca
    21241 gtagttgccg actctgtaat cggtgcataa tataatttat ccaatccaat cgttgccatt
    21301 ttcagacctc catttcataa tgcttggcga catcaatcgc cacatgatag taatttgtat
    21361 ctttttcaaa tcctacaaat aatcgttcag taatcgtaaa ctcgtgtttc aacaagttct
    21421 gaaccagttg tttttgcgtg ttcagatagt tgcctttcac aaacaaggaa atgcgaactt
    21481 cctgtacatc aacggttgga aagttatccc cgtaaacatc gaacctgtca gcgagtggtg
    21541 tcagcacaca atacttgtct ggcgctttat cactaaactc gcccgtttca acaggaatat
    21601 gaagtgtggc aaaaagactg ttcaattcct ctaaaatgtt caaatgccgt ttacctcact
    21661 ttccattttt gatttcattg tttcaatgca tgacttgcga ctttgtgatt ttgcaggttt
    21721 caagaatggc tttgctggct gaccgtgttt tccatattcg agaatgttcg caatcttagc
    21781 atttgaactg ccatcgattc gaggctcatc aaaaccaacc ttgatgttcc agttgccgct
    21841 tttatcttga cgagcttgtg tgataccaag ggctgattca agttcaccag ttgaacgact
    21901 tggtactttt gtatttgagc caacaacggc agttaagtta cttcggactt tgccttccac
    21961 cacttcagca ccactttcta aaaccttggg gatgatttca tctgtcttgt tttctagttt
    22021 tgaaaccttt aacaagaaat cttctggcat cttcatcaat gctttagcca tccgcactca
    22081 cctccatttc agtagtcagc atttcaaggt acatattgcg atttcggaca ttctccaccg
    22141 acacaatctg ataccttttc tgatctgaaa tgacaaccat ctctgtcgta acctctagat
    22201 taggaatata acgaatccga aacaggcagg tcgctgaact aaaagtcgcc atgtttaccc
    22261 atttctcagt cgcattcttt tcctctttgt atgcatggac agctgcaaga acttcatctt
    22321 gtttcactga aaatccagcg ctatcctttt ggttcgtcac tttaacgatt tgtattcgtt
    22381 gattcatctt tccaaaactc atacttgcca ccgcctatcg agcctgagta acagattgac
    22441 cgtttcccag acttgcttac tggcattgac gttatccgca aagaagccac cagttgaacc
    22501 atcacggctt tcgtaaaagt tactggctaa cacaataacc gcctgttcag tggttggcgg
    22561 catttcatgt tctgaatagt agccagactc aatatgttga tagctctcag cataagaaag
    22621 agcggtcaga atcaatcgtt ccaacagctc atcatcttcg ttatgctcaa gtatgaggtt
    22681 tgccttgacc ttatttagta agtcactcat aggctactcc ttaagatgag gcagatgctt
    22741 tcatcttcaa gatttgcact gcttcaggta acacaagttt accgtccaca cgttctttag
    22801 ctaagaaacc aaccatgcca ttacctgcaa agagttcacg caactggtcg aatgaacgca
    22861 cgccacggtc gccaatgtta tagtagctga aatcaccaaa agcaatgact ggcgcacctg
    22921 ccttaacggt tggaacaaac tccgaagtat aaactggata accgagcaga cggtcaggtt
    22981 cacctgctac caaagctggc tgccacatgt aggcaccatt gttgtctttc aacttacgaa
    23041 gcaagctgat ggtttggtcg ttcatgataa acttcgcgtt tttacgatat ggacgtttca
    23101 aagcatacac aaggttcacc acatcatctg ccgtaactgc actttgagta ttagttgtaa
    23161 tggcaacttg tccgccacct gttgctgcaa aaataccgag tggtttacca acaccgtcac
    23221 cattcaagaa cgcatcttct tccgcgtttg ctaaggcttt ggtgaattgg tcgatgatat
    23281 agttttcaag gttaaaggcg ttgtcataaa gcaattcatc agtcaccttg attgccacat
    23341 gaagtttgtg agcatcaagc aagatttgat cgaacgttgc atccccaaag ctcaactcgc
    23401 caccttcttc aatccatgaa gcggctggtt tagaacctgc aatattaatc ttgtgttcac
    23461 cacttgtcgt aattgttgtc gcaagtgtac gcatgatgtt ttcagaagtc atgccgtcaa
    23521 tcaaacgtga gtcatattct tctggcacca aataaccgcc tgccgtatcc acaccttctt
    23581 gaagaacatt tgaaacatgt cggaagtttg agcgcaaggc ttcaagtaca ccttctttat
    23641 attcagctga agcacgtcct gttttcttag gttccatttt attttcagta gtcataggtt
    23701 gcgaagttaa tggttggctc attggctttt caagttcacg atccatcgct tccattgact
    23761 gcatacgttc aatttccatc gtaaagttct tcacttttgt ttccatttcc gcataagtct
    23821 tggcatcttc ttcagacatc aagccatctt tatcttgctt tgcttctaca aaagcctttg
    23881 ctccgttcca agcttgtgca cgtttttcca tcaattcttg aattttgttc atgggttagt
    23941 tcctccaatt tttcattaaa ttaagccgac tcattaacga atcggctggg atagtgttat
    24001 taatttcttc tcgttccttt ggttcaggtg gttcaatacg acacttctta gcaatcgctg
    24061 aagtcagtga attagttact ttgacttccg agtacatcga attattaacc ggaatatccg
    24121 ggtcaatctc accgatgatt tcatcggcaa atcctaggtg aacagcttta tttgcgtcca
    24181 tccacgtctc cgcatccatc atatgtgcca gttgcacacg cgataaattc gttttacgtt
    24241 cataggcgtt gataatactt gctttcactt catcaagcat ggcaatggct ttctccatat
    24301 caactttttc gccaaacgca accgctgacg gattgtgaat catcaccatc gccacaggac
    24361 tcatgagtac cttgtcacct gccattgcaa taacacttgc ggccgaagcg gcgatgccgt
    24421 caatcttgac tgtgacttta cctttgtagt cagcaagcat gttgtaaata cttgcggcag
    24481 cgacacaatc gccaccaccc gaattaatcc agactgtaac gttacctcca cctgaattta
    24541 actcatcttt gaaaatctgc ggtgtgactt catcatcaaa ccagctctcc tcagcgattg
    24601 gtccgttgag gtagagggtt ctctctagga tttcctcttg gttgtcttga ttcagaaccg
    24661 tttgattctt ccacttccaa aacttcttca tcttgttcct cgctttcttc tttgtttacg
    24721 ccataagctg ccccggcttg ttcaattggc agcatattgc cattgaagta atatttcgca
    24781 ccagcaccac cagggattgg gttcaagtcc tcaagttcac gcacatcatc aggagatagg
    24841 aacccatttt gaataccggt tgcatagcct tgttgacgtg tggcatagtc accacgcaat
    24901 aagccatcca cattaaactt tatgaaatac ttttcttttt catcgcgact taacagtgat
    24961 ttattaagtg cttgttccca acgattgagc catggtgata atgagaactt cacaaaatcc
    25021 aaactcaaat gttcaacgtt tgagaaggtc gcgtggtcta agtcgccaac catgtgaagg
    25081 ggcactcgga atatccgtgc gatttcttcc agttgaaact tacgtgtttc aaggaactgg
    25141 gcttgttctg gtgacatgga aataggcgta tacttcatgc cttcttcaag tactgcaacc
    25201 ttggccgcat ttgaactgcc accaaatgtc gcattccaac tttcacgaag ctttcctggg
    25261 tctttcaaag ttccaggatg ttctaggaca ccaccaggat tggcaccatt ggcaaaaaat
    25321 ttagaaccat attcctctgt tgcaatcgca agcccgatag cattcttagc catggcaatt
    25381 ggtgaataac caaccaagcc atcaaagcca aggccaggaa tgtgaagcac gtcagctttt
    25441 gaaagtctga cttgcccgac atcagcttga tataaataat agatttcctt gttgtcatcg
    25501 cgattaaccg tcatcttatc tggcattaaa ggatacaaag ctacaacttc acctttgcca
    25561 tttcggataa tttgcgcata ggcgttaccc caaagtagca ggtgagtcat taaggtttca
    25621 cgaaatacaa atgatgtcat ttccgaattt ggttcatcat gcagcaagaa atacaacgaa
    25681 tgtttcattg ctttatcttt gccgccagca tctcggtatt ggtaaaagtg taatggcaat
    25741 cctgccacgg cttctgatag cacgcgcaca catgagtaca cagccgtcat ctgcatagct
    25801 gtttgttcgg tcacgctttt accagctgat gagccgccaa acataaatcg gtatggattc
    25861 atgatggttt gatttgttgg tttatcgcgt gatttgaata gtgattttat tatgcccatt
    25921 gattgctcct ttcaaaattt gagtatcaaa aaagcaccta cgatttgtag atgctgtgtt
    25981 cacattagat ataaaaccga cttcctcttg acaaaattat ctcaattgca ttatttaaaa
    26041 aggtatttac ttcctctaaa catttcgatt tttcgtcagg tgttaaggat tttctttgat
    26101 aaggaaagaa tgaactactt ggtgcaagtt ttttaaacgt catataatct tcatcatcga
    26161 agaaatcttg taaataattt agttctataa aacttttcca atctttgaat ttagagaaat
    26221 agtctttatt attaacgata aatttatcag cgttagttga tgaaccatat cgtttatctc
    26281 ttacatcaga ataaaataaa tcaggaaagt catgatacgg ccctttacct ttaacaccat
    26341 tgagagaagg ttttgataca tatagtggca taaaatttgc aagagtatga cttaaatcat
    26401 agaattttac aaatttcact ttcatctctt cggtgatatc atcggaatat aaaatgactt
    26461 tcaaacgccc ataatgacta tctggcattg aaaagttctt ttgattatag catttcaata
    26521 accgtatcgt tgcaccagca attgtgttga aagaaataat cgtttctccc ctataattcc
    26581 caattccttc agcatacttt gtcaaatctt cagttagaat ttttccccac cattttctat
    26641 aaaacagtct agaaattaat gtgttgtccg ggtctgaaga tattttatct ggtgctactt
    26701 gaatcaaata ttcattcata cacatacacc tccaaagaca attatatcaa tggattgtaa
    26761 taaaagctca tatttcacag aaacaacaat cccctatcat cataaacact ggcaccatta
    26821 tcatttccac aacgaattgc acgatccaga gccataattg tcgcgatggc accatcaatc
    26881 ttctcagtgg atttttcttt atcagctttg atattacctg ccgggtcagt tcggacgaaa
    26941 atgttgtcca tattccaacg aagaaccgga tgaccgccat gggctaattt ctgttcaaga
    27001 gtcaacttca tcaactcttt agtagccggg ctcatatcct tgaaaccttg accgaaagga
    27061 actacagtaa atcccatacc ttctaagttt tgaaccattt gaactgctcc ccaacggtca
    27121 aaggcaatct cccggatatt gtatttctca cccagttcct caataaattt ttcaatgaaa
    27181 ccatagtgga ctacgtttcc ctcagtggtt tgtatgtacc cctgcttggc ccacaaatca
    27241 taattcacat ggtcgcgttt aactcgtaag tccaaggtat cttctggcac ccagtaatat
    27301 ggcagtatca caaacttgtc atcctcatct tcaggtggaa acaccaaaac gaaggatgta
    27361 aggtcggtcg tgctggaaag gtcgagacct ccgtaacaaa ctcgacctct caaactttct
    27421 tcatctgcat tgaatgaaca agcgtcccat ttatccattg gcatccaacg cactgcttgt
    27481 ttcacccatt ggttcaggcg aagctgtctg aatgagtttt cttcagctgg attttgttta
    27541 gccgaatcac aagcagccac caccttttct tctgcaatcg ttatcccaag tgacggattg
    27601 gctttcttcc aaacttctgg atcagtccaa tcatcatctt gcttgatacc gtaaattaac
    27661 ggatagaatg ttttatcatg ctttcgacct tcaataatgt cgagagcttt ttgatgctgc
    27721 tcataacaaa ttgaattggt atcattccct gccgttgtaa tcaaaaagaa tagcggttgc
    27781 gttctagcat cccctgcccc tttcgtcatg acatcaaaca gctttctgtt aggttgtgcg
    27841 tgaagctcat caaatacaac cgcagaactg tttaatccgt gtttagagta tgcttcagct
    27901 gataatactt gatagaaact attcgttggc tgataaataa ttcgtttttg tgaagtcagg
    27961 attttcactc gtttgcttaa cgctgggttc tggcgaatca tatcaacagc tacgtcaaat
    28021 acgattgaag cctgcgctcg atcagcagca cagccatata cctcagcgcg ttcttcccca
    28081 tcaccgcaag tcaaaagcaa agccacggca gccgctaact cactcttacc attcttctta
    28141 ggtatttcaa cataagccgt attgaattgt ctgtagccat ttgccttaat cgttccgaat
    28201 aagtcacgga taatcttttc ttgccaaggc atcagtttga acggtttccc gaaccactga
    28261 cctttcgtgt gtttcaacaa ctcaataaag gccacggcat aatctgcacg tgacttatca
    28321 taatgggagt ctttgtccat aaattcagtt ggtttgtaat tatcgattgg aactcactcc
    28381 cttctacgca aaaagcagct gttgtcagct gccttaatct ctttattcca tcatatcggc
    28441 aatgaactga atggcttcag ggttgttctt gttatcccgc agcaattgca ccagttcttg
    28501 ccaatcgtag tatccttctt caatgccatc ttcattatct ggcatgtaaa tttgataatc
    28561 catcatttat caccctccaa gataaagcgg ctgtactcat ctttgtgttc ttcaatgaaa
    28621 atcaccaact cgtaaaagtg ccggtcatgt gcaattcgct gcactgcaat cgcatcaaac
    28681 atatttgtca gaccactttc tgcaatctca atgatttgtt gcttaatctt tgcgttcatt
    28741 ttgaccgtcc tttctgcctg cctcataggc cgcaattaag gcatcttcca aacaccaaac
    28801 cgcaatgtcc aagaagtctt cacggtcgct gttgtgggcc ttcaggtcgc cacgttcagg
    28861 caactgaacg atgtaattct ttgcgatgtt gtaaatctta cgtttactca ctttcatctt
    28921 aaagcccctt tctgatgtag atggtatctt ctgtttttga ctcaaggtca acccagacca
    28981 aatcgtggtt caatatcatg tccgtcacgt attgccaagc atcattgaag gtttcaaatc
    29041 cttctcttct accgttttga aagaccaatt cgaagtctgg tttgatgatg tgcaccacat
    29101 cttcgccgta taccgcgttc agtgttgaac cgttgtccca ttggataagg attgaaccaa
    29161 tgtcgtcaac accggttact gtgccttgtg tgcccttcgc tggggcatgt gggtcgttca
    29221 tcttgatgag ttctacacga ctaccttcag ggtaacgttt tttcataccc tcgatttgtt
    29281 catgagttgg aaacataact ttgtcttccg tattcttttg gtagtctata tatcactcta
    29341 aagggatttt atagcaagtg atacgttatt tattcaggat tttctaggtc attaaatgcg
    29401 atagtctttc catctcgaat cactgaaacg tctgcatcgc ttccagcctg ctcgatataa
    29461 cgcttaacaa tcacatcaca gaacttttca tccagttcga tcgtatggca aatccggtcg
    29521 gtctgttcgc aagcaatcaa agttgatccg ctgccaccaa aactatccaa cacgatgcag
    29581 ttgctgagtg acgaattggt aatcggatat gccaacagcg gaattggttt catcgttggg
    29641 tggtcaccat tcttctttgg tttatcaaat tcccagatgg tcgattcctt acgtccggtg
    29701 taccattgat gtttgccttt cttcttccag ccatagagca ccggctcatg ctgccattgg
    29761 taaggcgaac gaccaagcac tagactctgt ttcttccaga tgcacgtacc tgataaatag
    29821 aaccctgcat ccacgaatgc ttttctgaag ttcaagcctt ccgtatccgc atggaaaaca
    29881 taaatactgg cgtcgttggc catcacactt tccatacaag aaaaggcatc atacaagaag
    29941 ttgtaaaatg cctcgtcctt cagattatca tttttaatcg ttcccgcttt accttgatat
    30001 gagaccccat aaggtggatc tgtcaccaca agatttgcta gtttgccgtc catgagtttc
    30061 tcataagttt caggttttgt tgagtcacca caaactaaac ggtgacgacc aagcaaccac
    30121 aagtcccctg ttttactgaa ggctggcttt tcaagttcct catctacatc aaagtcatca
    30181 tcttgggcat catcattacc gttgagtaat ttatcaagtt catcagtttc aaaaccaagc
    30241 aaatccaaat caaactcttc accttgcagg tctgataact caaccgccaa catttcatca
    30301 tcccaaccgg cgttcattgc tagtcggtta tcagcaagta tgtacgcacg cttttgtgcc
    30361 tcggttaggt gttccacaaa cacgcacggt acctgcttga taccttcttc tttagccgca
    30421 agaatacgtc cgtgacctgc aatcaagtta taatccttat caacaatcac cggatttatg
    30481 aacccaaact cacgcataga agcttgaatc tgtttcactt gatccttact gtgggtacgg
    30541 gcatttctgg cataagggac cagcttatca atatcaactt tctcaaaacg ttctgttgtg
    30601 ttcattagaa caacccccat tctgctagtt tctcaaaccc gccaagctca ttcacataat
    30661 cacgcgcaac ttccacgatt tcagaataag gcacgccatc aatctcagtg tcaccaatcg
    30721 cacagcttaa ctcaacgggc ataccagttt cttgagcttt caagaatgcg tagatgttca
    30781 ctgacacatc agcttttgat aagtctttcc catggatacc gccacctgtt actccgtctg
    30841 ccatatcgga accaagcttg cgattagtcg cacctgtatc cacatccggg ccgccagtcc
    30901 aatcacctag agggttgact gtagcgtttg gatatttttg ttttaactca tttgtctttg
    30961 cgtggctctg acaaattatc aagcgttcac catcaagaat gtactttcca tcaaatggat
    31021 ggtcctgata taagtcacgc gcaatttttg agagctgtaa ctgttcatca gtcaatggca
    31081 tcccacgaaa gattccatta tcgccacaac gaatttcagt cgcttgattg tcagccaact
    31141 ctttatcttg gggcacaatc accacatctt ttttaatttt gcctgcgata cgattaatcg
    31201 cttggctgat ttcattcaaa tttaaagcag ttgaagtttc aataatcaca tgacagatgc
    31261 catgaccaat taaaacctca actgctatct ttggattatt ttcttctgcg tatgccaagt
    31321 ccacaatggc tccggcaata cgatctgcaa ctttatctgg atgttgcgga ttaaccttct
    31381 ctatcataaa ttctgttcct ttcaaccatt cttagaacgt aacaatcgtt ccatcgcatc
    31441 cgtattcgga tctcctgtgt attccactga tgcattctct tttacaatct gttgaatctc
    31501 ataccacgtc acatttgctt gcttttggaa gttctgcgac atcgccacaa atggggaggc
    31561 catgactgcc ttggtagtcg ggtgttttcc gagcaaacca tattgactca gagcattttc
    31621 acattgaaca aaacgcgcaa agttctgagc gtaggactca accattcgtg gggctatcaa
    31681 cgtttcgcat ccgcggtctt tcaaccattg gaacgttgag ttataaattt catcagcgcc
    31741 tagcacagtg ccatccttct gttttgcaga aaggtaagca cttggttcag gataatcatt
    31801 gccctcaagc tctacaccct caccaatatc ttcaccaatt aataattcaa catccggtaa
    31861 gtcaaattcc atccgcgatg ctttctttcc agcagcaact ttatcaacga gtggctgtga
    31921 gcgttcccct gaacgaggtc gtctgccacc gcgttgtgtt ccatcttttg ccaaaattta
    31981 tcacctcttt ttcttgtttg gggctatccc ccgtttgaac tgaacttttt tcgcgtaagg
    32041 gcctagcgcg ctgtacagtt tagaagggtt tagggataat taccgcccct cccccccttg
    32101 ttatttttca catgattgtt ttatcttttg tgtttcgtcg agtcatgtga attttcgagt
    32161 gacatgattt gcagacactc atcaaattat tttcatcatg cgtaccacca tcacttaatt
    32221 ccttgatgtg atgcacaatc tcaaccgaag taaagcgtcc ttccttcaga cacatctcac
    32281 ataggggatg tgctgccacg taccttgccc gtattttctt ccacgctctg ccgtaacgtt
    32341 tgttgattgc cggatcacgc tcgtatttct cgtaacgttt cctcgcaagt gttttatgtt
    32401 catcacaata agtatcatgc gtacggttcg gacaacctgg gaacttacag gggttagctg
    32461 gtttcattgg caagtggggt cacatccttt cgggcaaaag aaaagccctt gaaggatttt
    32521 tcccacaaag gcttcgagta tttgttcgat acttctattg ctggtattgc ttgagtatcg
    32581 tttgtctttt ttgacatctt aatgatatca ctaaaggcta ctgacattca ctgacacatt
    32641 tcaaatgact atgggttcat ctggtagctt caagttctca agagctttgc tatgccaacg
    32701 aaagactgtc cgcacgtcag cgtgaagatc ttcagcaatc ttgtcccagc tctcttccaa
    32761 caggtatcga ttgattaaca caatcttttc gtccacgttt gtgagttcat caatcacgcc
    32821 acgcatttgt cgtttgaggt ctacaaactt atcaatctct gcatcaattt cagcttccaa
    32881 ttggtaacac ttctcaaggt tacgaacaaa tggtgcctca gtattccgtg tgccacttga
    32941 aatcttttct tcgaatgttg tggaagaaac tgtcaccgcc agttgataga gctccgcctt
    33001 ctctttaatc cggtcgtcaa ttcgtttatt caaacggtac gcttgtttca agtattcttt
    33061 tggtgtcact tgcttccctc caatctagct ttcactgcat caatcaaaca tgattgtgtt
    33121 ttatcttttc gtttcaaagc caacatcaca tcttcatcaa tcgtatcttt cgcaataatg
    33181 tgatgaatga ccacagtatc actttgccct tgtcgccaca gccttgcatt ggtttgttgg
    33241 taaagttcca aactccacgt aagaccaaac catacaagtg ttgaaccacc agcttgtaaa
    33301 ttcaatccat gtcctgcact agccggatga atgacagcga tggggatgct accttcgttc
    33361 caatcaataa tgtcctgaga cgttttgatc tgtcgaacgt tgaatcgttc cttgatacgc
    33421 tccagatcat gctggaacca ataggcaatc agaatcggtt tgccatttgt accttcaatc
    33481 aaatcttcca aagcatctaa cttctggtca tggatgtgat gactcacatt aaactcatca
    33541 tagattgctc cattcgccat ttgtagcagt ttattcgaaa gcaccgctgt attggctgca
    33601 tcaatggtgg cttctttcaa ttgtagcacc aagtctgcct taatttcatc gtacttttgc
    33661 ttttctttga gtcccatatc aactggcact tcattcatca caagctctgg catatccaag
    33721 aaatcgactg acttcattga aatcgtgata tctgaaatct tgtcgtagat ttcttgttct
    33781 gcatcttccc ttggtttcca agaataaatc tgcatgccat tttgcttgtc aggtagaaag
    33841 tatttatctc gataacgtga aatataatag cccaatctct cgcccatatc cagaaccttg
    33901 aactccgcaa acaaatccat caaaccatta cttgaaggtg ttccggtcaa tccaacaatt
    33961 cggtcaacct tgtgtctgac tttcataaag ctagtgaacc gtttagcttt taagtttttg
    34021 aaggacgaca gttcgtcaat caccaccata tcaaaatcaa acgtaaaacc cgatttgtta
    34081 atcagccagt ccaagttctc acgattgatc agatagatgt tggcattatt ttgtaatgct
    34141 gccattcgct gacttggtgt tccaagaacc acggaatacg ataaatcttt caaatgatcc
    34201 cacttttgaa tttcagttgg ccatgttgat tgcgtcacgc gcaacggtgc aataattaag
    34261 acacgttgta ttgtaaagtc atcatgcatc aagtctttaa tagctgtcag cgttgtcacc
    34321 gttttaccca agcccatgtc caatagaagg gcggcagttt tatgatcttt gataaaatca
    34381 atcgcatatt tctggtagtt atgtggtacg aacttcatca gacatcacct cctattcttt
    34441 ctagtaactc aggtatctga tcaatctcat caagcacgaa acactgaaac cctaaatctg
    34501 tcagttgttt catacgaaaa agctgtaaag accgtggctt cttccctggc gccttcactt
    34561 cgacaaatcc aagtttccca tttggcataa gtaccaatcg gtcaggcaca ccagcaagcc
    34621 ccggtgatgt aaacttcggg caaataccac ctaccctttt gacggctttc actagtgctt
    34681 gctcaacctg cttttctctc atagccacac cgtcaaatac tcgaaatacg catcacgcaa
    34741 gatttccaag tattcctttt ctgcgcagtg aatcatcaga tgtgcgactt gcttatcgaa
    34801 tgatttcttc caaggatacg tgacatccga catggcaaac tcgtaaaact catcaaatcg
    34861 taaatcacgt ttaccgattc caatttgtaa ccactgttta aatttcattt agatattctc
    34921 caatctgcaa gtatggtgta acccgtgaat acgcaataca taactttata taggtggtaa
    34981 ttttttatcc cttaagaaaa gttctatata agacctacac aggttacact ttcctttaaa
    35041 atcaactatt ccaagaactc ggatttgagt tgcaaaccaa caatcttgtt tcctttatta
    35101 gttcgccggc gcttaaatcc gcccgcctcc agagccgtgt aaaaatcggc tgaactacga
    35161 atgtactcac cagttcgcgt acaaaacgca cgatactctg catacaactc accagatttt
    35221 tcttcaaagt caacagcagt ctcgcagcac tcgtctaaga aatggccaag ccaatcattc
    35281 gccgtcttat attcagaaat tgcattagcg acaacttgtg gaaccgttaa atgaaagtct
    35341 gcggcgatga cttttttcgc accgtcaagg atccattgca aaatggctcc gccggcattt
    35401 tcaaaaaggt agtccgcata attcttgatg tccttattgc cgtcaatcgt agcaaggaat
    35461 ggaatcacaa ttaaacgacg ccaggttcct ttatcaatcg ctccaacttt tgggaggtgg
    35521 ttggtataga gcaccaaagt atgcgttggg acatacttga atgggtcttt gtatttcttc
    35581 tcagccgcaa tttcatccgt cgagcagagc tgcttaatgt tagatgtgtt gagtcgcatc
    35641 ccctcttcaa gctcggctgc gatgagtagg cgtttcccct tcgcttccgc aagttcaggt
    35701 ttcacattgc gtcggatttg acttgtaaga atatcagctg agatgctgcc agagtaatta
    35761 ccgagcacac ggctgatgac attccagaac gtggacttcc cgttacgccc ttcaccatac
    35821 gagatgataa gcgcttcaac gtaaacttta ccaatagctg caagtcctac aatcatctgg
    35881 acataatcaa tcaactcttg gtcacctacg aaaatggtgt tcagagcgtc caaccaaatc
    35941 tgctgattgg cgtcactcgg ggcacattcc gtttgcttcg tgatgtaatc ttctgagttg
    36001 tggtcttgtg atttgcctgt tcgcaaatca aaggttgctg ttggtgtgtt cagcaagaac
    36061 tcgttcacat ctagtaaccg ttgctcgatt tcaagcatgg gctttgcctc ggttaatgct
    36121 gacgagagat acttgctgtc gcggcgtttg acagcaaact tcttatactc ttgtgcgtgt
    36181 tggtacaaat caaaactatg acgttgcgct gtgttgaaca tggtgactgc ctttttcgga
    36241 ccaacactag caagtacatc gaacgcgcca ttactaacca tttccttggt acgcttctca
    36301 atttcggttt cagattcttc cagttgacgt tctgtcaatg cgtggatcac accttgcgct
    36361 tttggtttcg actcttccca atagctgccg ttgtagacaa ggtaaccagt tgacggtgag
    36421 taatgaagct tacattcata ctcgcgagct aagaccgttg cttgacctaa gtccgagtag
    36481 tcggttggct caagtgataa atcagagttg tactgctctg gaggaatgta gccttcttgt
    36541 tgtttcactt tcttcccaaa gttgaccgcg ctattccaga tagctttaag ctcctcctcc
    36601 ggcagtggcg gattacattt ttgggattgt tcgagatact tgtgatacgt atcttccgtt
    36661 gcaccgtagc gtttgatgag ctttcctgcg atgtgactca tgcttgaatt acggctgcct
    36721 tcgctaatct ctgactgcgc attatcccaa tcagcaaaca gatcatcttc gagaaaatca
    36781 gtgatcaact tactaccttc atagatttca acttcagagt ttggtgttcc gaaaagcaaa
    36841 cgtgccgcat ccatcgcatt gttgtcaaag tatgggaact cactttgaat ctgcttcttg
    36901 agttccacat attcatcttt atcagttgtg gcaggaatcg ggaaatatac gtgaaaacgt
    36961 ggacgtgctg atttgccacc tttgtctttc atgttgttac gtgaatagct gaccgcaaac
    37021 gtcacatcag gaaactgcat cgccacttcg aaaggggcta cccaatcatc tggattgtct
    37081 gagtggtcat tgtcgcaatc aagtggaatc acatcagact ggatgaagtt gtcacctttg
    37141 cgatagttgc ctttgtactc agccatcaca tgatccagct tgaccgtggc aacaagtgtt
    37201 tctttatctg tcaccacttg cttggttggg aacaatccat tcttggaatt acccacctga
    37261 ttggatgtga ataacgtcat cttaagcatt tgagacctcc ttcatctcat catcgaagta
    37321 cctgatatgt ttcttccaat actccgcttg ttcaatttca actgccatgc cttgggagat
    37381 ggtgtcaccc accacccaga cttcctcaca tttgcccatg agaacaagtg acatgtgcat
    37441 tgccacatca cgctccgttg cgtcgtccat gaattgtgga tacatcagat gtgccgcaac
    37501 tggcatgtag ccacggtcaa gagcaatacg gcagtaacgc ttagtgtttt tatggttgac
    37561 gacaacattt cctgagaacg gcgagcagac atagactgat gttctgtaat ctttacgtct
    37621 aggcattatt atcacctcgc tcaatcactg gtaagatgtt cttcttcgat ttcagcaagt
    37681 cgtacaagaa caaacgacct ttctgcgtcc agtaggtatg catcacgctg cgctctgcat
    37741 ctatcgcatg agtttttgat tgtgtgtagc cttgattggc atacttctga taaagcaacc
    37801 acatcttgcc ttgcttgtac tgaattccca gttcatgaag caacttgttc attgcctgtg
    37861 gtgacatacc gtagtccttc gagattttag aaatcgacat cacagaattg ttttgaagaa
    37921 tcaaatcgta gtagctaact tttggtttca tctccaaaat catttgttgt tgcatcgcca
    37981 ccatttcttt tttctcttgt agctgcttca acactgaaat ggcccatgct ggatcttcga
    38041 ttgactttgt gatgaaatct tctgtcgcgt acacaccgtg ttggcggata gacggcaaga
    38101 cctcatttgt cacccaacgc ttgaatttct tcgcgttcgg catcttgcta gaaatgatga
    38161 gtgagtacaa tccactttcg ttgataagga tagccattgt cccattaacg ctgaacgatt
    38221 cgttcaccgt cttatcttcc tcatcaacat gatctcgtaa tgctttttgt ggatttgtgt
    38281 agccaagaat tgatgctaca tctttaccaa caaagtgagg gacatcgcca atcatcaccg
    38341 tgcgtacaga gccaaactcc gcattttcaa aaactttcaa ttcgttcatc ttgtgaacct
    38401 cctgtaatag aattttcagg agaggcattt tgccccttct aacttacagg cgaagaaaat
    38461 ggtcgaaagt aaactctgac tcgaatttat ttttgatttt tttgccttcg tctataagcg
    38521 aagaaagaac ccaattcgaa ccttattttt gataaaaaaa tttgaaaaaa tatggagcca
    38581 tttctgactc cacattgact aatccttttg atagaattca cactcgtatc catcagcgtt
    38641 cagtagcaag ccttcagccc acttaggcgt ttgactcatc aactgacaga tgtcttcaac
    38701 cttcgattcc attggcactt cgataatcac ttcgtcgtgg acgtgcccga caatgcgata
    38761 cttacgaaga ttctccattg cgaaacagag aatgtctcta gctattgcct gcacgatgtt
    38821 ttcgacaaac ttgggaccat agctttcaag acgttcccat ttcttggtag caccgacacc
    38881 ttcataggta acagcttcac cgccgaattt gttctcacca atctttggtt ttacataaac
    38941 taatttacgt cctgacaaga gttggatgaa cagaaaaccc gacttgtatt caaacttgat
    39001 gccatgcgtc gctgtgctcc gtcgttcttt cacacaagtc tttactgcct tatcaacagc
    39061 ccaccaaagc tccgttatgt taatgtttga gtctcgccaa gaattaacaa gcggctgtaa
    39121 ttcatcctca gttagtccca tttcaagagc atccattgct ttcaatgcac ccaccgatcc
    39181 tccgtaccct agagcaagct cagcaatttt ccctttttga cgtagatgcc cattcacgcc
    39241 atgcttttca actggcacac cgaacatctg actggcagac gcacagtaaa tatcgccacc
    39301 atttttgaat acttcttgac gccacttctc acccgacagc caagcaatca cacgtgcttc
    39361 aatcgctgag aagtcagata cgataaattt catcccttca cgtggtacaa acgctgtccg
    39421 gaggagttct gagagtacgt tcgggacatt gccgtagaga atgttaagca tttcaaaatt
    39481 gctttgtttt actaaactac gcgcttcaga taaatctgac atatggttct gaggcaagtt
    39541 ttgaagctgg atgccacgtc ctgcaaagcg tccggtacgg ttggctccat agaattggaa
    39601 taaaccacga gaacgattat cagaacacgc aaagttttgc atggcttgat actttttcac
    39661 ggatgacttg gcgagttgct gacgtaagac caggacttgc tttaaatttt catcaatagg
    39721 ttgttcgagt agttcggcca ctgccttttt accgagtgac tcggtttcaa gtccgtggtc
    39781 tagtaaccat ccccgcattt gaagcacgct gtttggattt tctagtccag ttaattcttg
    39841 caattgttga ttcaatccag cttttgataa ttcatccaac tgaacggcag cttccacaaa
    39901 gtccttatca atctcaattc ctcggtcgtt gatttcctga tccagccagt attctttcca
    39961 aacagaatca agcactggaa aattggaaag ttttccttgg atagctattt caacttccac
    40021 atcacgacta ttgtatgtct taaatgctga ccatttagtt ggcgcatggt gtggtaagtt
    40081 cctcgtgcgt ccaccattaa ctttggtcgg attgcatggc acgcaaaagt atcggatgag
    40141 gtcttttcct tctgtgagct tttgtttatc caaaccaaga acagcaccaa ctcctacaag
    40201 tgacaacggc aaacctaatg tcgcacccca caccattgtg cattgccacc ctcgtggatt
    40261 caaaaagcca tccaacccca gaaagcgtga caaacacaca cgttcaaaac tcgcattgaa
    40321 ggcgtgttta atcactgttt catctgatag cgaagttaat atttcagccg ggatagtttc
    40381 gtccatcttc agatcaacca cttcgacggg accaccatct acactgtatg caaatagcag
    40441 tatttcaaaa tcgggggact ctgaatattt gtagacacca catttcgata aatcgacact
    40501 actatacgtt tccaaatcca atgacaaatg tttcattttc ttcctccaat caaacaggcg
    40561 acagcacttc tgccatggcc gtttttgttt taatgattaa gccaagaaat catcatccgc
    40621 caaggtttca aactcatcgc ttgcagatac ttgtgagccg agtggttcgc cgtcacgtac
    40681 aaactggata ttcccaagcc ctgccgctac cccgcgattt ccatttgcgt taaaggcata
    40741 gaaattaatc gacacacgag cgtagcaacc actatacact tgtgaacgat ccaagattgg
    40801 ttgaacgtgc tgatccacga tttgtggtgc agtggttgag ttcgcattaa tgaagtaatg
    40861 gccttggtat gcttcatcgt cgcgttcgat atccccatca cgtaatggca gcttgatttc
    40921 tgctttgttt ggtttcttcc caccgaattt cgcaagtccc tcttcgattg ctgcatcgat
    40981 tgctttttca atggtatcaa tcgttctctt atctgatttt ggaatcaaga ctgctgttga
    41041 atatttctct gcgcccccgt tgatggatgc tggttcccaa ccgtggaagt atgagagacg
    41101 tgtgtttgta ccagtgatta ctttgcttgt tttagacata ttattgttcc tccgttaatg
    41161 gtttaaattc ttcgtttaag ttgtttactg tgaccgcttg tcgtttatct gaaccatcga
    41221 ctagcgtcag cttgcctttt ggtttgatga tgtattcgcc caagatttca ttgaatttct
    41281 ctttaccgag tgccttcgtc attgctgtga caccaagtaa ctttttatca tatgggtcgt
    41341 atccagcctt gatgaccgcc gcagcgacat cattgtcatt agaatactta cgagtagagc
    41401 gaccctcgac taatttgtga tgagtccatt gtttgccacc gagtgcaagt ttcaaagcac
    41461 agtccttgat atcgttactc caagaaacca ggtcatccac tttaccgatg atttcttcga
    41521 tttcatcatc actaagaagc tctggtggtt tgaactcata ttgcgccagt ttcaagttat
    41581 ggtctgctcg tgcacgacag ctattcttcg ccggacagaa tccacaccat ttcccgcatt
    41641 ggtactcacc ctcacctttg aaagctaaat ctgcggcagg cgacaacaca tcttttgccc
    41701 actgaagtaa ttcttctttc agcagtgtgt acgtactcac attctcacgc cttggctgga
    41761 aaatggtcat cttgacttct tgaatgtcat aaataacatc gaaaagctcc aacgctccca
    41821 aggcataaca catcatctgt gggttctgat acgcatctac caaaattcct agaccgtatt
    41881 tcatgtcaat aacagatagg gttccatccg caatgacgag acagtcgccg gtcccaaagc
    41941 cgtctgggac gtaccttgag aagtcgagcc gctgttctac tattacaata gagtcaactg
    42001 ttgtctgttt cgccttagcg acttcttcca tgacgaaggt cgcataactt tcagcacact
    42061 gctccatctc ctcgtcgtag aattcaagcg tagatgttgg gtctgtgacg tcattaccaa
    42121 tgaacttatt cagtttgtac tcacataagg tatgagcatc agtgccttgt tgggcatacg
    42181 ggctcgactt gttttcaaag tgctctgaca agcgaacact tggcggacag tgaatccacc
    42241 gattacttga agatgcggat aggactgcgt gttgtgttgg catctacaat ccctccgcat
    42301 ctgcaaccaa agccgcgtag ttttctgtcg gcacatcact caagcgactg gcaccatatt
    42361 tctcaatcag tgctttcact tcagttgact taccttcttg agatttacgt gccaacaaac
    42421 cacggacttc ttcaagcgtc ggttgtttga cttctgattt tgtttccatt gtcggttctg
    42481 gtgtttcaac ttgtggttca ggttcattgg ttttcatcgc cacagccaac tcttgtaagc
    42541 tatcagctaa cgcttggacg tcctcaacga cctgaagaaa tagttttgtt ttactcacgt
    42601 cattacgctc ccttcttgtt aacttcacgg atttcgactg attcgattgt gtcccctggt
    42661 gtgagcacga gaattttgtc catgttgccc ttaccgaaca gccaccgtgc aaaccagcga
    42721 ggcaaatgac gcacaccacc ttgtaggaca gtttctttgt tacccgtgtc atcagtcacg
    42781 tttactcgga ttttgtgttt taggttcatg atggtcctcc ttcatcttta tttgtttagg
    42841 atctactccc ttacacaata caggcgaagg aaaacgggga aagtaaactc ccccgtaact
    42901 tttttctaaa ataattcttt gatcttgtct ttgatgattc caaaggtgcg attgattgac
    42961 tgttttttct tgtcaagctt gtccgccact tcagtctgag acatgttttc agtgacgagc
    43021 atgtgataga tttcttggtg gtacggtaat aattgcgtca cgacttcacg caaacgatcg
    43081 atacgaggat cttcttcatt atcttggtaa gcatcgtaaa ccaatgattg gatggggttg
    43141 tccccctcat cgcattcatc atcaaattca ttgagcgaca aattgtagtt acgtttgaag
    43201 taatcgttgg cttcaccatc cacgtattcg tccattggat aatagccgtg ctcattatta
    43261 aagtcttcac ggaacttctc tttccaagcc ttgatctttt ctttttcttc ttctgtgcgt
    43321 tcaggacgtg cgtttttata aaagttgtaa atcgcacggt cgtcagcgcg atgcagctcc
    43381 tgaatgttaa gctcagtgac gccgtcttcg cctgggcgaa tctcaaccac ttctttttgg
    43441 atacgtccat tggcatcagt acgctcaaac acgtacttgt aagtgctgcg gtcgttttgg
    43501 tgtgttttca taaatctact cattagatcc ctctttcttg cctgaaagag cttttgagca
    43561 aggaaaacaa acagggaccg tgccaatacg tacaccgtcc ctcatcacga aaaaagagcg
    43621 caataaagta aggcatacaa tatctgaaaa attttctgga taaccagttc ttttcagtat
    43681 ttgtaacctt gccctattgc aactcaggca ttgttatatt ttattagtag gactattcgc
    43741 ttttaagata aattcgttca ctttcttgaa acataatgta gtgtttatga accataaatg
    43801 ataaaattaa atagttaaac tactatgttt taattataga aaaaggcctc ccttgttttc
    43861 gtcagtcatc ggcggcaaac ggcggtcaac ggcggacgta acaaaggagg ttgtattttg
    43921 gaaataagtg aaattactca aacgctttat ccgtttatgg gaagcgagta cgtgaagcca
    43981 caggattttt taattgcttt actggatagt gtttcaaatg atgacgaggc caccattttc
    44041 ggtttttctg atgataacct acgaagattt tacaatggta ctcgaccatt aacacaagag
    44101 tatgcagttc gggttgctgg aaaattcaac atcgacaaat gcacgacttt tatttatgaa
    44161 cgtcttatag atgatgctcc aaccgtcctt aagcaacaat tgattgataa aggtgtggta
    44221 atccccaaag atacagacgt ttctgaaata gtggcccatc ttttacttga tgaaatcacc
    44281 tcaattgctt ctcaaacaaa agtaaaaaca aaaaaggcca agccgaagct tagcgaatta
    44341 gagctcgcaa gtttaagaat caacactaat ggtgatttct cttataatga tgaagtgaaa
    44401 aaattctttg acgatttgcc agttcccctt gatattagag ctgaggagat gacgtatgtc
    44461 atagctcttt tttcagcata tgcagatgcc gatggatacg aagtatacga gcatacaaac
    44521 gacctgccaa attcgcggct aaaggatttc aagagacagc gaagaaatta ctataaagct
    44581 gaaagcatac gtcgtggggt ccgtgataac tttacacaag aggaaggaac tgagcacttt
    44641 gaagctttaa aagaagatat gtacgacggc atcgaggaag tctatgaaga agactacgat
    44701 gatggcgttg agagactcaa agccgttcta cagcagtcgt ctgttataac attaaacgga
    44761 tcaatcttat cgtttatacc gggattgctg caaaattctg ctaaaaaagg catttgccac
    44821 atgctggtta atgacggaaa gatcaaatgg gtgactgaag atgaatagtt tgtttaatac
    44881 tccatttgaa atttcggttc gagtaatgtt actgctctct gaatttaaag agcctgtcag
    44941 cgctgataga attttaatca cagacttcat caccacgtat ggtcgcgact ttgaaatttc
    45001 cgattacaac ttaaatggtg ataacagcta tagattcagt gaatttattg cgcgacgtga
    45061 attagttaaa gatgcagtca aaaatcttgt actgcaacat gttatccaac catcgcaaag
    45121 caaagaaggt tttaagtatg ctttatcaga agatggccta aagttgatat cacaattcgt
    45181 atcggtatat gcagaaaaat acttacaaat agctgacgaa tctgttaaat acattcaaga
    45241 taaaagtgac gttgagcttc tgcgacaaat aaaccaacgc gcaaaggaat ttaaaggaga
    45301 gcaatgataa tgcaaaatta ttatattgaa cgtgttacca taaaaggcaa agatcgtgaa
    45361 gatgcaactg tcaagttcac caatggacta aacataattt ctggcccttc aaataccgga
    45421 aaaactagca ttgttacggc aatcgatttt ctctatggcg cgaaaaaaga cgctgctccc
    45481 ttttcaaccg atgcaaccgg atacagtaaa gttgaactga ttattcaatc gactaatggc
    45541 aaatattcat taactagaga atttgatagc aactccattt tggtatccaa aataacgagt
    45601 tcaggtgaat atgtagatta tgaaaaatat aatgcacgat ctgggaaaaa tccaattagc
    45661 catttttggt tacaactttt aggcatcgat gatgattatc agattatcaa gaatgaggat
    45721 ttcaaacgtc aaaaattaac ttggcggaca ttagcgccta gcacacttat tccacaagat
    45781 agagtcaata ccagcgcctc tgttcttttt cctggggaaa caacccaacg cacagctttt
    45841 ttatcatcgt tgttacttct gctatacggt tcagattttg cgaactcgga agaaaacgaa
    45901 acaaaagaac ataaaaaaat tagaaggaac tcggtgatta actacatcaa ttcaagcctt
    45961 tctgaattat atgaaaacta cgaagtggct aaatcagaat tacctgaggc tccctctgat
    46021 gattttcaac aaattgtcga tggaatgttc gctgaacttg aaacaattga aaagcaaatc
    46081 aatgatgcgt tagccagcag caaaatcttg ataaaagaga tttatcaaaa ggaacagtat
    46141 cttcatgaaa ctcaacttct tttgaatagg tacaaacagt tacattatca atatcaatcg
    46201 gatattgaac gactaacact aatcgtagat ggtgagcgat tattaaatga tcataatgaa
    46261 catgatactt cacatagctg tccattctgt aacggcgaac taagtaaacc tgtccaaaca
    46321 acctacatcg atactgctag gtcagaatta cattcattgc tctctcaatt atcagatttg
    46381 acggaagaat ctcatgatac cgaagttgaa ctgcaagagt taatggaaca gcttgatgag
    46441 cttaaaacaa gaaagaaatc gcttgatgca gcaatcaacg atgaactaaa accgaaagct
    46501 gagacgttaa aggatcgaat caataaaatc gaaacttaca gggatattaa acaacagcta
    46561 atctcgatgg aaaaaatgca aaataagctt aaaagtgatc ttgaaaaaat cgagaatgag
    46621 ccagaagacg aacgtatcct ttataaacct aaagagaaat ttgatgcaga attctacaaa
    46681 gaaatgtctg agttactgga ctccatgtta cgtaatatgc attataaacc taatgaattt
    46741 cgtaacgcat ttttcgccaa ggatattttc gacatcaaaa tcaatggaaa tctaaaatgg
    46801 accgaaaacg gacatggata tacagcgttt atcaattcag tagtggtaat ggcatatcga
    46861 catctcttgt ataatcacgc taagtacaca ccttctgtgt tcattatcga ttcccctctt
    46921 cttggattag atgaacgcaa aaaacaagat ggcgatgaca atatgaaaga aggacttttc
    46981 aaatacttta tggatcatca aaacgaagga caacttatca tcgttgagaa tacaacgaac
    47041 ttgccagcac taaactatga tgataaacaa gtaaatcaga ttgtctttac tgaagataaa
    47101 aacaatggac gctatggata tctatacgat atttataact agaacaggaa tgattacatg
    47161 aagtttagct acaatccgct ttggaaactc ctaatcgaca gaggactgaa taaagcacaa
    47221 ctcagaaaaa tgactgatat cagttcagca acactctcaa aactaaataa aggcgaaaat
    47281 gtaacaacag atatcttact tcgcatctgt gaagcacttg attgtgattt atcggatatt
    47341 gttgaaatcg ttcgcgatta aatatcaaaa atgcactgta ttcgattcat gagtacagtg
    47401 cattttcata tttatttttg taatccagcg attacttcat tgattttcga ttcaatatca
    47461 ttcaatggaa ccatatacca ttcagtagct tttttgaagt ttccatcctg aaagacaact
    47521 tgtttgtcct ggaatacatg atggagagtg cgctcaagta gctgagcgtt gacattttgt
    47581 acttcagcag aaagcatgac cttcactggt gcatttaagt aggtcacgtc gttttccgca
    47641 ttggcaattc tactttcaac atcatttcga gtgaaaccaa tcttatacag gttattccac
    47701 ttgctaatat ccggattcgt gcttaatgac ttcagcacgt agatataacc tgtcgtaaac
    47761 tcttctccaa agctttcagc cagtgtttcc tcatctaccg gctctgtcac catccttcca
    47821 tgacgttcag tcgagtatag agacgaagct aaagattgta acaacagatt cttattctct
    47881 gtcccattct cataaacaac atgcattctg gcatttgttt tcccattatc agcgataaag
    47941 gtatccttcg aaatggaaac gatatacatc attacgccgt tttggatgta gaatctattt
    48001 tcatgaatat catactgctc aaacgggata atttttcgtc tgccagatgc gatatctgca
    48061 tgcacttgat cgaacaagct cttatacttt tcaaaatgac ttgcatgttt tctgcggcca
    48121 attttatctg cagcgttcac agttctacga tagcgggaaa catccaacaa gtctgaaaaa
    48181 tcatcaatca tatcatctga gcctaagata tcatctaaat tcaactcgtc atcgatttca
    48241 actgaatcag cgagaaactc tccatccggt tcgaattctt tctccaataa accaagagtg
    48301 tcatacttct taaccttctc agacctttcg ggttttcctc gaaatccgct aagacgagcc
    48361 cacaatgcgc gttcactaga ccattcagga gttttttctg gctctcgtcc gttttcattc
    48421 acaaagtcta taatctcctg aaatttagca acttctgggt cttgttttac aactttcttt
    48481 ttttcaactt gcttggttaa atcatcaaaa gcgtcatctt caaagatgct atctaaacta
    48541 ttcgccatct tctttcgctg cctctctttc acgttttatc tcagcaagtc gtagcaaagc
    48601 aaaggccaag tatttttcac catcatcttt actatttcga tcaggatttc gaccattttc
    48661 tgcacggaac ttcttagcag cttggtaggc cgcaaccaat tgttcgtcag taaaatcata
    48721 cttatttgat gacaaataat cctgaataaa ccgaagagtc ggtgcatcaa tatcacgact
    48781 aatcacttca tacgcacgtt ggaacggatt cacttgatcg attaaatcaa tattcaattc
    48841 atcaatgtta atgaaacgat cggccatctt aataatttcg cggctaccat cttctgaagt
    48901 tgttgtccgt ccagccgcaa tatttatatc ggcaactgta tgttgacgaa cggtttctaa
    48961 ctcttcgttc gtcaaatcag gataacgatc aatgatgaca cgtggaatca acgtcttatt
    49021 aatcatcttc gcatcagcac ctgaaacaat cgcattttga atatcttggc tctgtaaaat
    49081 gctagatttc aaatcttcca tatcattttc gatgattttc tttgtacgtt ccgtcgtatc
    49141 agcaggtttc agtcctctga ttgaaagatc acctggtttg tctggcttac catcttttgg
    49201 tttaaacttg taaactggcg ccaacacttg ttccataagt agagatgcag aaattgcctt
    49261 cataatactg ttaaccgcga ataatacttc gtcatcctgt gcatctggct gcgcaataag
    49321 atttgtaaac tgtgcatgag tcttattggg gctgtcacga gttacgcgac caatgatttg
    49381 aatcacttca gttaatgagt tacgataacc gattgtcagt gcatattccg caaatggcca
    49441 atcgaagcct tcttttgcca ttccaagtgc aatgatgata tcaagcttat caagatcttt
    49501 aatgtcatcc gtaatacttg ctagatatgc tgagactttt tcacgtcctt cttcattcac
    49561 aaggtcggcc actaatagtt ttttaccatc tggacgttcg atttcataaa taccatcctc
    49621 gttctgtgtg atattaccac cgtccgtcaa taaatcaagt atttcatcaa cttccgcgta
    49681 tttatcttta gtagactcac cagaattaac attaggaata tggataattg attttttgtt
    49741 gacatcaatt actttatcca aagcatctgt atattttcca cgatagaaac tataccccat
    49801 tgcaaaggat ttcagatatt tatatccttc cagttgctca taataagtgt aagtgacctt
    49861 atcaaattta tcttcgtcgg ctggttccag aatctgaaca gagtcgccac ggaaatatga
    49921 accagtcatt gctaagatat gcgcattaga gtttgacatg atgctacgta aggcattacc
    49981 taacacagat ttatcatcac ggctcacatg atggaactca tcaatcgcta atagcgtatt
    50041 gtcaaataat gaatcgtcca actcttcaaa agcaaatcgc aacgtagcat gtgtggcaat
    50101 caggatatta tcacttgggt cagtcgagtt catgaattta acaaagtgtt gaacctttga
    50161 tttttcaccg cccccaatag ttaagttgtc acttggcttt acttcccaat cccaataaaa
    50221 accaaacttc gataattcag tatctttaaa tgatttacca attgagcgtt caggaactgc
    50281 aataaccact tttttaattc cttgattatg gagtttatcc aagccaacaa acatcaatgc
    50341 acgtgatttt cctgaagctg gtggtgattt taccaacaaa tactgtgacg cacgcttttc
    50401 ataaacacgt tcttgcattt cacgcatacc gaggtcattt gttgtttttg attgattttt
    50461 atcaaaatta atttcaaata aattactagt cattgattgt catctcctga tagagtttta
    50521 gtaatgttcc aagtcgatct tcgtcagatt caaacgggcg ttgctgataa gctctatcaa
    50581 caatcccatc cagttcctgg tgtttcttac gtaatgattc tggcatactg tctttattat
    50641 acaaatcggc taacgtgccg ccttcgtatt ctcgtaaatc aagaatttct gtcatcacgc
    50701 gagtcatttc atttttacgc tgggcggata aatcctgaat cggaaaagta ttatagacta
    50761 acccggctga ataacggtac cgagtttcta atttcccacc gacagagcgc agccacacca
    50821 tatgcattcg ggattctaac agaccaagca gccacattgg agcgttatag attgccatag
    50881 ccgaatctga aattatagta tcgctcccaa ctatacccat aggaacataa tctctttttt
    50941 ctgatgacac acgaggaatt agaatactat tctcactggt ttcatcataa cgaacttcac
    51001 caaatgacca tggcttctca gctagtgctc gagtttcttt tctcttgctt ttttccctat
    51061 aactttttac attttttatt gtttctttaa taagagaatt ttgtttgtat ctagttgcat
    51121 cttcctcggt caaccataaa gtatatctta agtctccatt tattaatcct gttgatccta
    51181 aatatttctt aaataaatgt ttaatatctg gatatttttg aatcaaattc cagtattctg
    51241 ccactgaaaa aactagatta ccaccatcat ttgccatact gccaaatttc aaagagggta
    51301 aatttgaaat actatttctt gattcgttga caaatacatt ttcaccatta gctaaatatg
    51361 cattaatatt tgaaacactc attactgacc cattctctaa atataactta ggatgaatat
    51421 tcacattatt tgtcatacca ataatgacaa cagtcacacc ggcattattc tttgcattgt
    51481 tgttccactt aaagctctga tatgcaaatg aaattcttac ctcatctttt aaaaggtacg
    51541 gccaaaggat actaacttgt tcaccttgac aaattgaatt agtagtaaca aatgcgaacg
    51601 agccatgaga tgttgcaata tattgtttag ctttccaaaa ccacccaaaa atataatcaa
    51661 gcaatttata ttttcgaata tcgccaacaa gtttttccat atcttcacgt tgttcagcat
    51721 tttgaagact tttgcccaga tacggtggat ttcccattat atatatttca tgattttctt
    51781 tgagtcgcaa cacctcattc caatcaattc gaagagaatt ggcacacact atattcccag
    51841 catcttttaa cggtaacaac tcagcaatgg tacctggtat tgcttcttca agctctttat
    51901 tcatttgatg ttcagcaatc cataatgaaa gtcttgctac ttcatgcgca aaatcatcca
    51961 attcaatccc attaaaatta ccaagatgaa tcactgatag tggcatggaa tcattatttt
    52021 gtaagtcgcc caacaattgc aaaatcttaa tttcaagtct acggatttcc ttataagcaa
    52081 tgatcagaaa attaccagaa ccactggcgg gatccaaaaa cttaatatta gaaattcgcg
    52141 tcaacaaagc acttagatca ttgatgattg ttctcttatt atcgtttctt gttttttcag
    52201 taatgtcctt cagctcgttt tcttcactgc gaagctttaa agtttcaaat gactctgtca
    52261 aatcatctaa gaataacggc ttgatgactt tcatgatatt cggcacgctt gtataatgca
    52321 taccggcaac gtgacggtct tctgctgaag caactgattg aatcattgcg ccaaggatat
    52381 caggattgat ttcattccaa ttcaataatt cccctgcttc aataagcagc tctcttgaaa
    52441 cttttgaaaa ggtcaaatcc acatgtggct caccaaacag ttttccatta acatatggaa
    52501 attcaagtag ccactcatcc ttgccattgc gagctaactc attaatatcc aagattgcaa
    52561 agagttcttt gattacttcg ttaaaattcg aaccatcttc agccgttctt gtttttagaa
    52621 catttgtaaa ggaaccctta ttcataattc ctgtatcttc tgcaaacaac aagaacagca
    52681 ctcgaatcaa gaacaagtta aatgcatgct cgttggcatc aggattatct ttagccacca
    52741 catcatacaa ttttgcaaaa cgttcagcag cttttcggtc agctggattt tcagcttgat
    52801 aatcagcctt ctcaatgcca ttccacgcta agaaaaagtc agcattagcc ggtagctctt
    52861 caattggaat attcaaagtt tcatgagttt tcgtatcaat tgcagcaata tctaaataat
    52921 cgtttaccaa gatatatcgt ggtttagatt tttgctcttt gattgattga gcaatcgcat
    52981 caatcgcaat gacaacgttg ccttcaattt cttcataatg aagtttgttc ttaatcacaa
    53041 atggttcacc attatcgaat tttgattttg cacgtgtaat cgacgttttg gggatatcgt
    53101 acaaggacaa aaattgagtg atgaaatcat cgcccctatt tgaaagtatt ttttttacac
    53161 catcttcaat ttcagtgatg tttaaaactt tattttttgc catttattta acatcctccg
    53221 catctataac atctgaacta attatttcac taacatcaac atttaaggca gcagcaattt
    53281 tcaataatga ctccattgca accaactcat ttttaccgag ttttgctatt acattgggac
    53341 tgacgccaga tatttcacga agttgagttt tgttcatatt caaatcaatc aataacttcc
    53401 acaatttatt ataagaaatt gacatactac acctctattc tactggtatc ttcaattata
    53461 tcatctatga gctatcatta ttatacgaaa catgaaatat tttcgcgaac gcaaactatt
    53521 tttctaaaat aaaagccttc agcatagatt tactaaaggc gagataacaa atattaattc
    53581 cactttattt taaccggaat ccccaattga cgagctagtt tatcaatttt gattctgaca
    53641 ttctttttgt tgaaatttgc ataaacccat actccatcag ctaccctggc tgaatttggt
    53701 cttccgttat acatggatgg tttacgtcct aaaattatag cgaacgacgg cgtctcatcg
    53761 acaattaatt cttgcagttg attgttgtag tttccatccg caaatagctg agaaacaaaa
    53821 tcaaccaata aacctctagc agatttcgaa gatatctttt tcccatcata ctcaatttca
    53881 tattgtccag atgaactaaa ttcataaatt tgggtttcac tgtcttcgga agtaccaaac
    53941 aaatcgatat ttgcaatttc atctataatt tcaaacactg tgtcatatag ctgatccgat
    54001 tttgcttttt gaagtttatc aatatacgaa gtattaccag cagttgaatt catcatttca
    54061 tagtcagatt tttcttggaa atcttggata agtcgttttt caagataatc tgtatctgct
    54121 tttgacattt tcccatctgc tcttgtaaag aataaaactg attcaaccca agctttatat
    54181 tccttgagaa agtgttgaga taaacgaagt gaaattgatt gccctgctgc ctgacccacg
    54241 taacgcttat taccaccaat caaaacataa acagctggat aatttgaaaa atcctgttcc
    54301 ttcaaatacg gcaaatcact acgactcatt gaaaccaaga ttgctggctc gcgttcaaaa
    54361 taagaaatat tatctttctg tgtatctgaa aatataaaaa tttgcttcat aagtttgtct
    54421 cccttgaaaa ctcgataata tattatatca cgtaaccgtt atcaaaatca tctaacctat
    54481 caagtctacg gtgtaactcc tatcaactca aagctccata gcatttccat caactctacg
    54541 ctcacccaaa aatcgtcatt tcaacacgaa aaaagagcaa tccggaaacc atcccgaatc
    54601 gctctaatcc ttgtcatatc aacgttccac acgtgtaagc agtcctacac actcgatgtg
    54661 atgggtttgc gggaaaagat ctacaggctg aactttcttc acggcataac ctgcttctgc
    54721 aaataactta gcatctcttg caaaagttgc cggattacat gagatataga cgattcgttc
    54781 tggtgacatt tcgattgctg attcaataaa atgatgatct aatccttttc gtggagggtc
    54841 aactacgatc acagatggtt tgatcccttt ttcttgccat tttttcataa ctttttctgc
    54901 ttttccgact tcataatgaa cattttcgat tttattattt aaagcattga actgtgcatc
    54961 ttcaattgct tctggaataa cttccatccc atacacctta tccactctgt ctgctaaaga
    55021 caagccaatc gtacctattc ctgaataagc gtcgatcaca atgtcttttt tatctaactc
    55081 ggcaaactcg atggctgttt ggtacagata ctctgcttgt tgagtattta cttgataaaa
    55141 tgactttgct gagattcgat aggtttttcc taatagctga tcttcgatat agtctctgcc
    55201 atataaaact ttttctttgt ctcctaaaat cacatttgtt ttttcttcat tgatattttg
    55261 gatgaccgaa accacttctg gcaattcttc atgaatagct tcagcaattt ccttggattt
    55321 aaagaatttt tctttacgag taactaaaac gaccatcatt tcatgggaat aatgaccacg
    55381 acggataatg atgtgacgaa gctgtccttc atttttttct tcattatatc ctcgtacttg
    55441 gaaccgttgc aagatatctc tgactacttt gattgcttga tcgatgacag gatcttggat
    55501 ataaaaatct tcgataggga ctagatcatg gctattcttt ttgtaaaaac cggtggctaa
    55561 ctgtccatcg atacggcgaa cagggatctg tgctttatta cggtagccga atggttctgc
    55621 cattccgacc gttggaagta caggaacttc tggcatctta gcaatcttcg ccattacatg
    55681 ctctacttgt ttttgtttga agacaagctg ttgttcatac ttcaaatggg ctaaaggtgc
    55741 gatacctatg cgaagtaatg cattgttttc tacaggctga cgatcaggtg attgtgtaat
    55801 aaatgtttcg acttttccat atccaaactt attgcctgct tttactacta gtatttctac
    55861 ttcttctcca gggagtgcat tttcgataaa taatggataa ccatcgattt ttgctacccc
    55921 cattccttca taactcaagt cgacgatttc gactgtatat cgttcgtttt tctttactat
    55981 ttgctgttct gtcatatttt gctcctttaa gcctattttt caatcctact gaactctact
    56041 ataaatcagc taattacaca actaaaaccg gcactgacct tactacgttc agcaccggct
    56101 tttgattaat tcttttgatt aggtgtttcg tctgctattt ttccgtcacc gtcgatatct
    56161 tcatccgtta aacgttcgac ttcttttacg aattctttgc tgacaacctc taattcttct
    56221 tcatctgtac ctgtgattgc ttcatcagga atctgatcgg tattggcaaa gaactcaatg
    56281 tgttgatgaa gattttggaa atgcatcggt gcttccccgc cgtattcacc atctaagttg
    56341 atcattactg gtcgattgat atcatcttct gtttcaacga acaaactgct tgttttggta
    56401 tagattattc ttggatcatc gacatgtcga ccgccattca gcatgagtgc tgcaatatgc
    56461 aagatctcaa agatatttgc tgtcttcaca atgattaaag aaaacttccc atcatccaat
    56521 ttggcatctg gcgcgatctg ttcgaatcct ccaacagagt tcgtcagccc taggaaaaac
    56581 atcgaagcat ttccgatata ttccccttca tcatacttca agcgcatttt gattggcttt
    56641 actcgaggga gcatctctgc tcctttcgca agataagcaa gataaccaaa aatactcttt
    56701 agttctgatg gaacttcata agtcaattct gtcaaatagc cacctgcagc gatattgatg
    56761 aagtaactgt tatcagaaag gccgatgtcc atttttacgg tttggtgttt ttttatcact
    56821 tctgctgctg ctttaatatt atctctagga atttttaatg ctcgagcata gtcgttcgtt
    56881 gtaccagctg ggatgatggc cattttagga cggtgttcaa gcggagcgat tccattaacc
    56941 acttcgttga ttgtgccatc gccaccagct gccactacta gatcaaaacc tagttctgcg
    57001 actctacatg cttcattctt ggctgaatct ggttctggtg tagttgcata ggcgctggct
    57061 tcaaatcctg cttcttctaa tgcctgtagg atatccgcca aatttttttt caataattct
    57121 tttcccgagg tggggttata aattacgcgt gcttttttca ttttcagcct ctatcgtttc
    57181 gctaattcat ctttaagtag cttattcacg acaccaggat ttgcttgtcc tttagttgct
    57241 ttcatgattt gaccgactag gaagccaacc gcacgatctt ttccattttt gaaatcatcg
    57301 acagattgtt ggttgttatc caaaatttca ttaatgattg gcagtagttt gttcggatca
    57361 gataattgca cccaaccatt tttttcaact acttcttggg cttctccgcc atttgtgatc
    57421 aattcacgga agactttttt ggcgattttt gagctgatcg ttccatcggc gatcaaacga
    57481 atcattcctg ctaagttctc aggtgtcaat tttgtctcag ctaattccag tttctcactg
    57541 ttcaagtaag cagaaacttc acccatcaac cagttagatg cttgcttggc atctgcacca
    57601 ttttccaaaa cagcttcaaa aaaatcagac atttcttttg taagtgtcaa taccattgcg
    57661 tcatactctg gaagtccaaa ctcacggata tagcgttcgc gacgagaagc tggcatctca
    57721 ggcaaactag cttttacttt ttgaatccat tcgtcatcga tcacgaattt cgggatatca
    57781 ggctctggga agtaacgata atcactagac ccttctttga cacgcatcaa aactgtttta
    57841 ttcgttgttt cgtcaaaacg gcgtgtttct tgttggattt ctcctcctga aagaagaact
    57901 tttgcttgac gtttttcttc aaaagctaaa ccttttttca cgaaagataa agagttcaag
    57961 tttttcaatt ctgctttcgt accgaactct tcttgtccat aagggcgaag agaaatattg
    58021 gcatcacagc gcatagagcc ttcttccatt ttcacatcgc tgacttctgt aaattggata
    58081 attgatcgca atgcttctag ataagcgtat gcttcttctg gtgaacgcat atcagcttca
    58141 gaaacgattt cgatcaaagg cgtgccttgt cggttcaaat caacgtaaga ataaccgcca
    58201 ataccatgca tgttttttcc agcatcttct tctaaatgga cacgttcgat ccggattttt
    58261 ttctttttac cttctacttc gatttcgatc catccgtcat gaccgattgg ttgatcaaat
    58321 tgggaaattt gatatgcttt tggattatct gggtaaaagt aatttttccg gtcaaaatga
    58381 gtatctttag agatttcaca gtttaatgcc aaagctgctt tcatcccgaa ttccagcgca
    58441 gctttattca ttactggcag cacgcctgga taaccccagt cgatcacgtt tgtattgctg
    58501 tttggttctg caccaaaatg agctggcgct ggagagaaaa tttttgagtt ggtttttaac
    58561 tcgacgtgga cttctagtcc aatgactgtt tcaaaattca ttcgtctttc cctcctaaaa
    58621 tagctggttg ttgtttatgg aattctgttg cttgttcaaa cgcatatgcc gcttgataca
    58681 tcgttttttc atcaaaataa ttaccgatga tctgcaatcc aactggtaat ccttctgata
    58741 aacctgccgg tacagacata cctggaagtc cagccaagtt gacagggatc gttaagatgt
    58801 cactcatgta cattgtgatt ggatcgttaa tgttctcacc gattccgaat gcaactgttg
    58861 gtgaagatgg cccgatgatc aagtcataat ctgcaaagac tttttcaaaa tctcgtttga
    58921 tcaatgtacg gacttgtccc gcttttttga agtaagcgtc ataataacca gcacttaagg
    58981 agaatgtacc taacatgata cggcgtttta cttcttcacc gaatccttct gaacgtgagt
    59041 tgacatatac atcatctaat gatttcacat tttctgagcg ataaccgtag cggataccat
    59101 cgaatcgttg caagttggaa cttgcttctg atgaggcaat gatataataa acagccacac
    59161 cgtattttga atgaggcaag ctgacttctt ccacaacagc acctaatgaa cggaatgttt
    59221 ctgctgcttt caatacagct tcttttacgc caggggccac accttcgcct aagtattctt
    59281 taggtaaagc aatcttcatt cctttgatgt cgcctgtcag tccctctgca aaatcaggaa
    59341 ctgaaacgcc tgaagaagta ccgtcttttt cgtcataacc gctgatagct gtcaatgcta
    59401 atgcattatc tttcaccgta cgggtcattg gtccgatttg gtcaagagat gaagcaaagg
    59461 caatcaaacc aaaacgtgag acacggccat aagtaggttt catccctacg atgccgttaa
    59521 aagctgctgg ttggcggata cttcccccag tatcactacc taaagaaact ggaatttgtc
    59581 cagctgcaac tgctgctgca gaaccaccag aagagccacc aggtactttt gttgggtccc
    59641 aagcattttt tgttttcttg aaataagaag tttctgtact tccacccata gcaaattcat
    59701 ccatgtttag cttaccaaca gggatcatat ctgcttgata gattttatcc atgaccgttg
    59761 catcataaat tggttcaaaa ttgtaaagga tcttagatgc agcggttgtc aagatatctt
    59821 ttgttacgat attatccttg ataccaattg gaataccagc aaggacgttg ctttcatcaa
    59881 tccctttttc atctaaagct tttgcctgtt ccaatgcttt ttcttcgttc aatgtaatga
    59941 atgcttcgac ctcaggctct gtttctttga ttcgttcaaa tgttgctttt gtcaaatctt
    60001 gtacagtgat ttctttcgat acaagcatat gatgcagttc ttctagagaa cgattgtgta
    60061 attctgtcat tatgcgccag cctccccatt atcgatgatt gcgggtactt tgataaagcc
    60121 gtcttctgtt tccggcacat ttttcaataa ttcatcgcgg ttccatccag cttgcgcccg
    60181 atcttgtcgc atcacgttta ctgtttcaac cacattcgac gtaaatggaa caccttctgt
    60241 atctacttct tccagcattt caaccatatc gatgatttta cctaattggt cggtaaaact
    60301 tgcaagttct tcatctgcga acgcaagttt tgctaatttg gcaacgtgtt tcacttgttc
    60361 ttcactaatc gccatactac cccttctttc taaacgagtt aaaaactttg ctctctattt
    60421 taacacaaga gtgccgtgtc tgaaattgtt tattgaaatt tgtctgaaaa aactcatttt
    60481 tcattcttct actgagcgac ttttagccaa tgcatctgcc atttgatctt ctgtccaaac
    60541 ttcgattcct aattcttgtg ctttggtcag cttgctgcca gcatcttctc ctgctaccac
    60601 gatatccgtt tttttcgata cgctaccagt cactttcccg ccaagatttt cgatcgtctc
    60661 ttttgcttct tcacgagtaa atcgtgttag tttaccagta aggactactg ttttttcttt
    60721 aaaaggagac tcgacttctt gcaattgggc atttcgaagc cctttgtatt cgaaattgac
    60781 gcctgcttgt tttaattcat tcatcaattc atggacttct tcgttactga aataagtgac
    60841 tacactgtcg gcaatgatat caccgatcgt atcaagcgca ataatcgact catagtcact
    60901 tttactcaat gtttctagat caccgaaatg ttctgctaaa atttttgctg ctttcgctcc
    60961 gacatgtcgg attcctaacc cgaaaatcaa ccgctcgacc gaattatctt tgctgttgtc
    61021 gattgctgtc aaaatattat ttgctgattt ttcttttatt ttgtccaacg tcaataactc
    61081 ttcttctgtc aatttgtaaa gatcagcaac atctgctact agttttttat catacatctg
    61141 ttctagtacg cgcggcccca acccatcgat attcatcgca tttcttgaaa caaagtggtt
    61201 caaaccttct ttcatttgag cagggcattt aggattgatg caccgtaagg ctacttcttc
    61261 atccaaatgg acaagttcac ttccacaaac tggacaatgt gtaggcaatt ggtattcttc
    61321 gctgtcttta ggtcgtttat ctaacacgac ttgcgaaact tctgggatga tgtctcccgc
    61381 tttataaatc agaactgtat ctttcaagcg gatatccttc aacttaatat aatcaccgtt
    61441 atgcaggctg gcacggctta ctgtcgttcc tgctactcgg actggtgtca tgatagctgt
    61501 tggcgttact acccctgttc gaccgactgt ccattcgatg ttttcgatta gcgtttcaac
    61561 ttcttctggc ggaaacttgt aagctgctgc ccatctcggt gctttgacag taaaaccaag
    61621 ttgatcttgt agcgagaatt cattcacttt gatgacgatt ccatcgattt catagggcaa
    61681 atccacacgt ttgtcatgat attcttcaat gtatgaccac acttcatcaa tcgaatgaca
    61741 tagacgtttt tcccgattcg tgtggaatcc aattttttct agctcttcta gcgcatcata
    61801 ctgggttttt gcttgcatag gaccgaaatc agctactgtg tataaaaaag tgtccagatt
    61861 tcttttcgct gtgattttag aatctaactg acgcaaagaa ccagctgcgg cgttccgagg
    61921 attcgcaaag atatcttttc cctctgcttc tctgtcttgg ttcaattgga caaatgaccg
    61981 ttttggcatg aaacattcgc ctcttacttc gatgttcatt ggttctttta atttgattgg
    62041 gaccgaccgg acagttttta ggttttcagt gatgttttct cctacagttc catccccgcg
    62101 agtcgcacct ctcacgaaat ttccatcttc ataacgcaaa gaaacggaaa gtccatctat
    62161 ttttagttca cagcaatagt caactacacg tcccactgct ttttgtacac gttgatcaaa
    62221 agctatcagc tcttctttgc tgaaaacgtc gttcaaactg taaagcggaa tatcatgagt
    62281 gactttttca aaaccttcta atacttttcc gcctactcgt tgagtgggag aatcagacgt
    62341 gatcaaatca ggatattgtt cctcaattgc tactaattct gcgtattctt tatcataaac
    62401 atagtcttcc actgttggtt tatcttctac gtagtattcg cgagaccatt ggttcagtct
    62461 tgtacgaagt tcttctgctc gtttagcagc ttcgtcaatc gtcatttcat gttccatcat
    62521 ttaccctcca ctactaagct ttttcaattg gcgcaaaggc agccaaaaga cgttttactc
    62581 cttgacttgg gaaagccaca tctagttcta gatccttcga cgtcccgctt actcggacga
    62641 ctgttcctag accccatttt ttgtgtttga ctttatctcc aggtttccag ctgttatttt
    62701 cccctccagt agcactttta gagggaacaa ccgctgtctc tggctgttta tagctatatg
    62761 ctgttttttt agcgaataca ggtgtttttt taggcgtagg acgcatgcct tcgatttcca
    62821 gtaactcctg atcgatttct tctacaaaac gagaaggtcg gttatattgc gtccgtccat
    62881 ataatgttct ggagaaagca tttgttaaat acaacgcttc ttctgctcgc gtgatcccca
    62941 catatgcgag acggcgttct tcttccagtt cgctttcttc catcaatgct cgagataaag
    63001 gaaaaacccc ctcttctaga ccaatcagga atacaactgg gaattctaat cctttggctg
    63061 catgcaaagt catcaatgtc acttgggaag catcttcttc taaattgtca atatcggaaa
    63121 caagtgccag atcatttaag aagaccgtta gtttttcttc tggtgcatca gcgtcttctt
    63181 cattttgctg ttcgaattgt ttgtcgaatt cttgtgtgac cgttaaaaat tcctccaagt
    63241 tttcgatgcg tgcttgtgct tctaatgtat tttggatttt taagtcttct agatatccgc
    63301 tacgatccaa aacctctttc gtcaattctg tcactgtcaa gtatgggatc atctgagtga
    63361 cttcttggat catctctcca aatgcaccta attgctgtcc tgcttttcca ccgatatttg
    63421 ccagatccac attttgcgct gcttcaagca gtggccattc atgaagagaa gcaaaactgc
    63481 gtagtttttc gatagatcct ggtccgatgc ctcgtttggg cgaattaacg atacgctcaa
    63541 aactgattga atcttgagga tttgccagca cattcaaata cgctaaaata tctttgattt
    63601 ctttacgatc gtagaacttg tgccctccaa ccattttata aggaatattt gctttcaaca
    63661 gagtttcttc cattacacga gattgtgcgt tcgtccggta caagatggca aagtcgccgt
    63721 aattccgatg attgcttcga atctcttctt gcatccgatc tacgataaaa cgggtttcgt
    63781 ctcgttcatt atctgcacgg tagtaagtga tcttgtttcc ttctttgttt tctgtccaca
    63841 agttttttgg ttttcgattg ctattgtttt caatgacttg gtttgctgcg ctaaggatgt
    63901 ttttcgtgga gcgatagttt tgttctagca aaattactgc agcatctgga taatcttttt
    63961 caaaatccaa gatgttctgc atatctgcac cgcgccagcc gtaaatactt tgatctgcat
    64021 ctcccacgac gcacaggttc ctaaatctac ctgccaataa attgactagc gtatactgtg
    64081 cgtgatttgt atcttggtac tcatctacat gaatataatg gaatttgttt tgataatatg
    64141 tgagtgaatc aggatgttct tcaaataatc gaatcgtatt catgatcagg tcgtcaaagt
    64201 ccatacattg attatattga agctcttttt ggtaagcagc ataacatttt gccgcaatct
    64261 cttcgtaata actgccttgc atttttgcaa aatcctgagg ggtctgcagg ctgttttttg
    64321 cttgagagat tgttcctaat atacttcgag gatcatattt tttcggatca atgttcagtt
    64381 cttttagtat ccgtttcatc aaggtcagct gttcagaaga atcgatgatt gtaaagttcc
    64441 ggttataccc gataaaatca acatctcttc ttaaaatacg cacacacatc gagtgaaaag
    64501 tcgataccca gacatcttcg cctccagatg cgagaatcgc attgacacgt tctttcattt
    64561 cttttgcagc cttattggta aaagtaatcg ctaggatgtt ccaaggattg acttcttttt
    64621 cctcaatcaa ataggcaatg cggtgtgtca gcactctcgt ctttccactt cctgcacctg
    64681 ccattagcag taatggtccg tcagtgtgaa gaacagcttc tttctgacgg gggttcatcc
    64741 cgtttaataa ttcagctttg gatgacatta acaacatcct ttcttttatc aatcttgtta
    64801 attatagcat tttcttggct ttcattaaac tagctagttc aaaatcattt gtttcttttt
    64861 atggattttt ccttgtcaat tcgttgattt cgcgctaaat taaaagaaag cgaggcgttt
    64921 gaatggaaaa aaaaggaaaa atcaagaaat tagataagac tcagatatat tttattctaa
    64981 aaggaatatt tgttggactg ctgacaggaa tcgtcgttag cctgtttcga ttatctatcg
    65041 aaaagctaag cgatttagtt cgaagtatct atgaaatgag tcgggaacag ccgatttact
    65101 taattggcct agctatttgc tgcatccttg cagcattttt cgtgggttat ctggtcaaaa
    65161 atgaaccgga tatcaaggga agcgggattc cacaagtaga gggacagctt cgcggggaat
    65221 tatcaatgaa ctggttctct gttttatgga aaaagttcat tggcggtgta ctttcagttg
    65281 gtgcgggcct ttttctaggt agagaaggac cttctataca gcttggcgca tccattggac
    65341 aaggagctgg acaattgttt cggagtccat cttcagaaga aaagatttta atttctagcg
    65401 gtgcaagtag cggacttgca gcagcattca atgctcctat cgcaggatta ctctttgttt
    65461 tagaagaaat ccatcactcc ttttctccac ttgtttggct gacatcattt gcttcagcaa
    65521 tcactgctaa cttcgtctct ctttactttt tcggcttgag acctgtttta tatctaggtg
    65581 accttccttc tttgccgttg aaatattatg gttccttagt gatcttagga gtacttctag
    65641 gcatcttagg ttttatttat cagaaggtct tgctggcttt gccaaaattt tacaaccgat
    65701 tgcctttacc agcttatttt cacgggttcg ttcctttctt gctgattttg ccaattggtt
    65761 atttatggcc tagtactcta ggcggaggca atcaaatcgt tttagctttt ggacatgctt
    65821 ccttgccaat cattatgatt atcggtttgt ttgttcttcg attcgttttt tccatgattt
    65881 cttacggtgg tggtcttcca ggggggattt tccttccgat attgacattg ggggctttaa
    65941 tcggtagctt ttatggaaac attatggttg atttcgggat ggatccaatc tacatcaagg
    66001 attttgtcgt tattgccatg gctggttatt tcacagcgat tggaaaagca ccgctgacag
    66061 caattatttt agtgacagaa atggttggct ctcttaatca tctgatgcct ttaggattgg
    66121 ctgctcttgt tgcttatatc gtcaatgacc ttctcggcgg taatccaatt tacgaatcat
    66181 tattggaacg tctacttgga aatactcaga ccgcctcact aaaaggtgca aggacaactt
    66241 ttgattttcc agtaacggca gagagttctt tagacggatt aatggtacga gacttcaatt
    66301 ggcctaaaaa tatgctgcta atctctatac gcagaggaaa tcaggaaatc ttgactcacg
    66361 gtgatacgat catgaatgta ggagatattt tggttatctt gactgatgaa gcaaacttgc
    66421 caagaatcaa gcaggaaatc gaggctaaat cttctatcac aaatttgaag caatcatttt
    66481 aacgaacatg gaaaaactat tttacaacaa ctattcatct acactacttg attctatatg
    66541 attctataaa aaaagaagag actgggaaaa accagcctct tcttttttat ttctatgctt
    66601 ctgctgctgc aggagctttt ctcaataaac cataaatcac accagatacg actgctccga
    66661 tagcaataaa caccaaatac atgaccgggt gactcagtaa gaatacaacg aaaatcccgc
    66721 catgaggagc cagtaatttg atcccgaaag ctccgactaa tccgccagtt aaagcagaac
    66781 caataataaa acttggaatg gcacgaatcg ggtcagctgc tgcaaatgga atcgatcctt
    66841 ccgtgacaaa cgatagtccc atcacgatgt tcgtcaaacc agcatcttga tctgttttgc
    66901 tgaatttatt cttaaacaaa cgtgtagcaa cgaaaattgc caatggaggt accattcctc
    66961 cagccataac agaagccatc acgacacttc cgccagttgc aacagtcgta gctaaagttg
    67021 ctgtaccaaa cacataagcg gctttgttga ttggaccgcc taaatctgcc gccatcattc
    67081 cagctaataa tgcaccaagt aaagcagcat tcgaaccact catacttgtc aagaaaccgt
    67141 tcaatgcatc attgattgct ttcataggga cattgaccag caacatcaag aaaccagtaa
    67201 tcagtaagcc aaacactgga taaaagagga tcgttttaat tccatccaat gattttggta
    67261 agttcttgaa taatttacgt aaaaatacga tgacataacc agctaagaat ccaccaatca
    67321 gtgcacctaa gaatcctgca ccgcctgtat tagctaatgc accagcagca aatccaacga
    67381 tcaaaccagg gcggtcaccg atacttgaag caataaatcc tgctaatacc ggcagcatga
    67441 aaccaaatgc agcttgtccg atttggttga accagctagc agcttgattg taattcccta
    67501 gatttgataa ttgatcatgt ggcacgccaa taaattggtc gatcatgaaa gaaagcgcaa
    67561 tagcgattcc tccaccaata acgaaaggta acatgtgtga tacgccgttc atcaaatctt
    67621 tgtaaattct ttgtccgata gtaccatccg catttccttc ttctgaactg ctttcttttc
    67681 catcaccatg gaatactggt gcagtaccac taagggctag attgatcagc tcttctggtt
    67741 tacggatacc atcactgaca ggacgattga tcagttcttt tccgtcaaag cgattcatct
    67801 ctactttttt gtctgctgca acgattacac cttctgcacg ggcgatgtct tcagcagtca
    67861 atcggttttt gattccttct gaaccgttcg tttcgacttt gatctccaca cccatttctt
    67921 tcgctttttt cttcagagca tcttctgcca tgtaagtgtg agcgatccct gttggacaag
    67981 ctgtaacggc aacaacgaat tttctttctg gatgagcaga agcttctgca gcttgtgttt
    68041 cttgttctgc tttttcttct gcttcttttt gctcttcagc tgtttggaac aagtcttgga
    68101 cttcttctgg tgtatttgct tcttttaatt ttcctacgaa atctgcatca atcagcaatc
    68161 gagatagtgc tgctaatgct tgcaaatgtg tgtcgtttgc gccttctgga gctgcgatca
    68221 tgaaaaacag ataagttggc tgtccgtcta aggcttcata atctactcct ttttggcttt
    68281 ttgcgaataa aactgttgct tctttaactg cactgttttt cgcgtgaggc atggcgattc
    68341 catcaccaag acctgtagaa gtttgtgctt cgcgggctag aattccttct ttatatgttt
    68401 cgatatcgga aatccggccg gcatcaaaca ttttttggac catttcatcg attgcgcctt
    68461 ttttatcagt cgcttgcaaa tccatgatca tcgcttcttt gaccattaaa tccttgatat
    68521 tcatgattat cctccttgaa actaatcaat ttttgtgatt tcaacttctg gcagcaactc
    68581 atcaatcaat tcgcgagctg ccaagtcatc agaaaaagct gttgcgcttc cgcaagcaac
    68641 tccccattta aaggcttcga ttggatcttt cgtacggctg taagccccaa taaagccggc
    68701 aatcattgaa tcccctgcac ctactgaatt tttcaatggg cgaactaata cattcgtttt
    68761 ataagttcct tgttccgtga agagcaaagc accgtctcca gccattgaga tcagcacatt
    68821 ctgtgcccct tcagctagca attttttacc atacggaatg atttcttcta cagattggaa
    68881 ggttgtattg taaagctcag ctaattcgtg attgtttggt ttgatcaaca atggtttctt
    68941 aacaagtgca ttttttaggt catcacctgt tgtatcaatc acgaaatctg cacctttttc
    69001 tcgaatgata tcaatcagtt cttgataaaa tccttttcgc aggctagccg gcgcgcttcc
    69061 tgacaatacg acgatatctc cttgtcctac tgttcctaat gcttgcttca actcatcgat
    69121 ttcttcatct gaaatagatg ggcccaaacc gttaatttca gtttcagtat ctgattttag
    69181 tttgatattg attcttgtat cttcttttac ggttgtgaac aaagactgga tttgttcttt
    69241 ttcaagccac ttcgaaataa attcgccagt gaaccctcct aaaaacccta gtgctgtaga
    69301 ttgagaatcg atccgtttca aaatacggga aacattgatc ccttttcctc caggtaactt
    69361 gaagtcttcc ttcattcggt tcaaatcgcc tagttttaac ccatctacgc gtacgatata
    69421 atcaattgat ggattaagtg ttactgtgta aatcattgta ttgcctcctt aattgttgtc
    69481 ttttcacgga attcattaac gaattctaat ggacaacgct cggttatgat cgtggctgct
    69541 tccaaaggtg caacagaggt gaaggacaac tggttgaatt tggtatgatc aatcaataca
    69601 aatgtctctt cggaattttt catggctaaa cgtttaacag ctgcctcttc tgggtccgga
    69661 gtcgtgaatc cttgttcaag atgggcaccg ttcattccca taaatgcttt attaaaatga
    69721 aaatttttta gttgttctat cgcattagat cctaaaacag cattagttgc cagctttatc
    69781 gttccaccta aaataatggt agcaagctcc aaatcaacta aatttgccgc atgttgaacg
    69841 gaatttgtca ctactgtgat ctgttttcct tgcagaaaag gaatcatttc taacgtagta
    69901 gagccagcgt ctaaataaat gacatcttcg tcattaatgg tttcagctgc cgatttggcg
    69961 atttgctgtt tttcgtgaac gtttttgagt gatttttcac tcatattctg ctcaaaattc
    70021 aataattgct ctttcttagc tccgccatga actctttcta gaaacccact attctccagc
    70081 tcttgtaaat cacgtcttat cgttgattca gaagcattaa aaaggtttac aagatcttgt
    70141 gatttcacga ttttctgctg ttcaagcaag tctaggattt tttgtctacg ttcctctgta
    70201 agcattttca cgatcacctc aatacaaata atagcatatt ttttttctac tcgcaatata
    70261 aatcagtcaa aaacgttcat aaaaattata aatcagtcaa aaaaaaataa aaaaccagtc
    70321 aaaaagactg gttttttatt tttttgtcta tcatcaagcc gttttatcgt tttttatttt
    70381 cgcttcttgt ttttttcata taaacagaag caggttttct ttccttatga agaagataat
    70441 aataaagtcc catcccgcca aacatgacca aaaaaccgat gactgatcga ataggaaaat
    70501 taggaacaaa aaagagacag acggataata atgatgccgg aaccattatt cgataataga
    70561 gttgtttatc cattttcttc acctcatatt ttttgtaaag taatcaattg ttctaattct
    70621 cggtaatcta aaaattgcac acgcagatgc tgctcacctt tcttgacatc gaattggata
    70681 cttcgccgct ttttctttat tcgtttgaag gtaacatcag agagtctatg tttgatttcc
    70741 tttaacggta attctgacag ggattctgaa ggaacataga ttcttaagga taaaagatac
    70801 tcttgatatt tttcatctgt tacctctact tctagatatt ccctaatata ataacaataa
    70861 gcttcgattc cattatccaa agcaacagtc ggatcgataa ctttatagca catcatgcgc
    70921 gagcgattct cgtcaaccca aggaaaatac gcctgagcat ttagtgttcc gcgaacacgc
    70981 ctttctttat aatctccttg ttgaatatca taactttttg ttacaaaaaa tgaagcattc
    71041 tttttaggaa caggttgcat ttgtgcattc tgatactgga cagattggat cgtatggtca
    71101 gtgtaaatgt attcaacgaa tggcggtttc aataaaccga caccattgtc ccaagcaaga
    71161 tgatcaaacc caagctggtc cctaaaatag gattgaaaca tttgattgtt tggcttttgc
    71221 cagttaaagg ggtctcgata aagctggata catatgactt cgttttctaa ttccttcact
    71281 ttttctactt gttggatgtc gagaaaaatc ggtttaggga tgatttgatg ataatagcga
    71341 ctataataca tttcccaaca cgaagtgaaa cataaacctt ggtaaaaaag atcgtatcct
    71401 ggaaattggt tgcaatcgac tactacttcc ccgttttgtt gtttcatttt aggtaaatgt
    71461 tcgatttcat cctttttctc aaacaatagc cgttgagaga tctgttttgt attgtgatag
    71521 ataaactcgc tataagctcg catataggcg aacaccccat gagcaatcat ttttttctga
    71581 atgaagtctc gaatcaaaaa ttgcttttct ttgaaaacag agatagacaa tacatttttt
    71641 atcaataatt gattatcttc caagcgaaaa ctacattcgt tctcgtcatt tcctacaact
    71701 aaccaacttt cgtctcttac cgatgctagc attatttttt cttgaagctt tcgaagatca
    71761 tacggatccg cttgttgtgt gaaagagcgg ataaatgttg gctggatcaa cagacgtgtc
    71821 aaaatagccc aatcgcttat ttcctgtttt ttttcttctt catttaagtc tgtgagctgg
    71881 atagtcatca ataatttatc ttcgccaatc attttctcac ctctttaaaa tctttttata
    71941 atttaattat aaccaacgat aaagaaaaac taaaacttta tgtcttagaa accaatttgg
    72001 acgattacat gatagactaa gtaaaaagaa acaaagaggt gtttacaatg gggttgaaat
    72061 ttgaacgtac agataattta gataaacttt tcgaatcatt tgctgtcgat ccggaaaaaa
    72121 cagttgcacc aaaagaagag atcgatcgat ttctaaaacc acagccacaa aatgaaaaga
    72181 aaaaagacaa gaaacaaaag taagttaagc tcaaaaaaag acttaccagc caaactttta
    72241 tgaaagagtt tgactggtaa gtctttttta ttctttttat gattcaattg cttgttcgat
    72301 tgctgcccac gcttcttctt ttccctgttt tgtaacggaa gaaaagagaa taaacgtgtc
    72361 attgcgatca aaatccaacg cttttttgat ttcgctctca tgcttgttcc atttaccacg
    72421 agggatctta tctgccttag tggcaacaac aattacaggg atatcataat acttcaaaaa
    72481 ttgatacatc tggatatctt cttgagaagg cttatgacgc aaatctacta aagaaacgac
    72541 cgcacgtagc tggtctctag tcgttaaata tgtctcgatc atctttcccc attttgctcg
    72601 ctcagttttg gatacttttg catacccata tcccggaaca tcgacaaaat gtaatgcgtt
    72661 ctcgatcaaa taaaaattca atgtctgggt ctttcctggc ttaccggaag tacgggctaa
    72721 attctttcga tcaatcaatg tgttgataaa agaagatttc cctacatttg atcgtcctgc
    72781 cagagctatt tctggcaatt gtgtatctgg atattgtttt ggtgccactg cacttatgat
    72841 aatttcagct tgatggactt tcatggtctc tctccttaac aaggtgatga ataaagcaag
    72901 cggctgtcgc aaaagccaaa atgactttta cggcagcctc ttttcatcaa cccgcttttt
    72961 tctctgaatc tttatagatt actgttggct caccagacaa ttcagctgct tcttttgtga
    73021 taatcacttt ttcgatgttt tcatttgaag ggatatcaaa catcacatcc atcatgattt
    73081 cttcaatgat ggaacgaagt cctcgagcac cggtattgcg ttcgattgct tttttcgcaa
    73141 tggctctcaa cgcttcaggt tcgaattcaa gttcggtatt atctagagaa agaagttttt
    73201 gatattgttt gactagcgca tttttcggct cagtcaagat ccgtaccaga tcatctgttg
    73261 tcaatttctc taaagctgcc ataacaggta atcgtccgat aaactcaggg atcaatccga
    73321 attttagtaa atcttcagga atgatatgct gcatgacact ttcgtcttca gaaagttttt
    73381 ggttttgtgt accaaatcca atcgtctttt cgcccatacg attcttcaca atcgtttcga
    73441 tgccatcaaa tgcaccacct acgatgaaca atatgttcgt cgtatcaatc tgaataaatt
    73501 cttgatgtgg atgtttacgt cctccctgag gtggcacgct tgcaacagtt ccttctagaa
    73561 ttttcagcaa ggcttgttgg acaccttcgc cggaaacgtc acgagtaata gatacatttt
    73621 cgcttttacg cgcaatttta tcgatctcat caatatagat gatccctttt tctgcacgtt
    73681 ccacattgta atcagcagat tgaagcaatt taagcaaaat gttttctaca tcttccccca
    73741 cataaccagc ttctgtcaaa cttgtcgcat ctgcgattgc aaaaggtaca ttcaatgttt
    73801 ttgctaatgt ttgtgctaaa aatgttttac cagaccctgt aggtccgatc aagcagatat
    73861 tgcttttttg cagttcaaca tcatctgtgt tttcttgtgc ttgctgattg acacgtttat
    73921 agtgattgta tacggcaaca gctaacgagc gttttgcgcg atcttgtcca atcacgtagt
    73981 tattcaatac ttcaagaatt tctagtggtt tagggacttc agtgaattcg cgtacagctt
    74041 cttcataaaa ttcttcatca atgatttctt tacataggtc gatacattca ttacagatgt
    74101 agacccctgg accagctacg atctttttca cttcttcttg ggttttcccg caaaaagaac
    74161 agcgtacggt ctcattgcta cttggattgt catacatggt cttcacccct taacttgcaa
    74221 aaagtatgca agaaactatc aaagacttta cagtctttta ctatcataac atagtttagt
    74281 aaaaagaaaa agtgtttaac aaaggatcaa ctgacctata cctttgcatt ttttactctt
    74341 aacaaaaaat aaggaactgt gacaaaatcg tgtcacagtc ccttatttcg aataaacaat
    74401 tttctagaag tagcttcttc agaaatgatt tattacttga tttctattct ttcgcttctg
    74461 tcgctgtacc tgtgatcaat tcgattgctt ttttcattgt cacatcatgt tccagcatat
    74521 cttctgtcaa cacgcgtttg atttgttcga ttggcatgtt gtatgtttct gataattctt
    74581 ggatttctgc attgatttct tcttctgttg cttgaagatt ttcagcagct gcaattgctt
    74641 cgatcacaag atttgttctt gtgcgcattt ctgcttcacc ttcgaattgt ttatgcaagt
    74701 cttcttctgt agaaccagtt aattgatagt acatttcagg agagatacct tgacgttgca
    74761 tgttgttcaa gaattcgtcc attgaacgat gtacttcatc atgaaccatc acgtgtggca
    74821 attcaacgat ttctgcgttt tcaacagcca tacggatcgc tgcttcatct tttgcatcgt
    74881 ccgctgcttt ttcttttgtt tctactaatt ctttgcgata tttttctttc aattcgtcta
    74941 atgttgatac ttcgtcatca acatcttttg cgaattcgtc atctaattca ggcacttctt
    75001 ttgcttttac ttcatggatt gtcactttga agactgcttc ttttccagca agatcttctg
    75061 cttgataatc ttctgggaaa gttacattta catctagact ttcacctgat ttatgaccaa
    75121 ctaattgatc ttcaaaacct ggaatgaatg agcctgaacc taattcaagt gagtaatttt
    75181 cgccttttcc gccatcaaac gcttcgccat ctacaaagcc ttcgaagtcg attactactg
    75241 tatcgccatt ttcagctgca gcatcttctt tgataactaa ttcagcttgt gcttcttgtt
    75301 cacgtttgat acgtgcatcc acgtcagcat ctgttacttc acggtcttgt ttttcaacaa
    75361 ctagatcttt atattgtcct aattttactt ctggtttcac tgttacttca gcagtgatta
    75421 cccagtcttg acctttttcc atgctttcaa catcgatttt tggttgagaa acaggatcga
    75481 tgccagcttc tttgacagca gcttcataag cttctggtaa tacagcattc aatgcatctt
    75541 catataaagc ttcttcaccg tacatacggt tgaatacttg acgagaaact ttccctttac
    75601 ggaatccagg tacgttcaag ctttttttca ctttattaaa cgcagttgtt aatccttttt
    75661 ggatttcgtc ttgcgcaatc gtaaatttta aaacaccatc attcgtgcct tttttttcaa
    75721 atgttgcagt catttaattc cctccgagtt tagaaattca ttttatacat caaaaacgtt
    75781 tcatgcatac ctttaaatag tacactaacc ctttcaaatt gtaaacatat tgaatggata
    75841 gaatccttgt ttttcataac atttttatct ttttcactga taaaatctaa tggaagaaga
    75901 tcatttgctg caaaattttt tctctttttt ttaaaaaatc ttgtttttca tcttctgtac
    75961 cttcatacat cgtttttcct gtacacgcct ctacagtcag ttgaaaccag cgcttcgggt
    76021 cttcgatgta tgtatcatag acaggataaa caattgcaga ttgcaacgtg aattcttcat
    76081 ttaacacgga actaagcagc gcatcttcat taccgtaacg atctgaaaat acacataatt
    76141 gctggtaact tgcctgctgc tgcggctttg gcagatcttt tggtatgact ttgactaact
    76201 tttcttcaat cgtcaaatag aaaacaggat ggcattcgtt caattgccta agtgtctcca
    76261 gtaattttgc ccttgctaaa atatggacct ctggcgatac aataagttta ctcgctgttt
    76321 ggataaactc atgataagga agctgttcga tctcatcaat agacgcaagc tgacgtgcag
    76381 gttccaacga aggaagttcc tccgatttat tgtggagtgc cttgatcttc tgcctctcca
    76441 tttgtccgga taattcttct gcaaattgga ttttttcaat gacccgctgc tcgaaagctt
    76501 cataaaaatc tgtacttgct atcaattttc tagcatataa aaaatcttgt gtactgatta
    76561 acaagtcaaa ataaaattca gcaaactcct cattttgaag atactgtttt tcatgcaaga
    76621 gtgcttgctt caatgcttct tttttctcgt ccaactcgta aagacaaaaa acaagcaggc
    76681 tattagcctc aaagctttct ttcaactcgt aagctcgttg aaataattct tttgcctgct
    76741 gatattgctg gtttgctagt gcttgttgtg ctactcgcat taaattctca tatttttctg
    76801 gaaaatcaat cggcttcacg cagtgtcctc ctcttgctca tgttcagctc atgttcaata
    76861 gtagcaaaac aaaaaaagca tgacaattaa aaataactga agcaccctct aaaagctaga
    76921 ttttagatct gacttttaga aggtgctttc aactgttact gctttttatt gctcatcagc
    76981 tgttgacttc ctggttgaat gctttgatca gattgatcgt aacaacactt gtattagtga
    77041 aaaaatcgcc tagatgttgc tttttctgat taaagattgc tggaagatac cctaacatca
    77101 gcaaaggaaa ttttagaatg aaccggcaag caccttctct aactaaaacg gttgaccagc
    77161 taagttcttc ttcatcaaag ctgatgactc gaataccaaa gatcattttc ccaatcgttt
    77221 gaccatgatt gaatttcgtc aataaaataa aatacgctag ataaataagt aaggctgtga
    77281 atccgtagat actcagataa gaatctgaaa aattaagcca acctagattt ttggcaatac
    77341 caagtgttaa acgcgtaata gctccgatac acaaaagatc gaccaagaaa gcaaacattc
    77401 ggataaaaaa acctgcatag aaatacttag gaaaatcatt tgcttttggc ggttcatttc
    77461 ttgtgtccgt atactttttc catttacgtt gtttcttttt gatttcttcg ggcgtcaaag
    77521 gctctctttc aaaagagctc agtacctttt gggaaaagtt ggagtcaggt cctacagcag
    77581 gctgctgttg cttatctttt tcttgttcct tattttcatt tgaaagctct gacatgctta
    77641 ctcacctccg taataataca ttgcttttgg tgattccgct gtccctagat tctcaagaac
    77701 ggataagatc tgactagtct cagaaggctt caatccctgg aattctgcta gtttacttcc
    77761 taaccatgta ctagcaaacc cagatggcgt ggaagaatat tctactagtt ccgcatcttc
    77821 aagctgttgt tcctttcgca tagcttttaa agcatcttca ggaaatccca attcatcgac
    77881 taaacctact tcttttgctt ggacaccatc ataaatcctt ccatcagcaa tttttttgac
    77941 ttcttcttca gatttattcc gtccctcgct gacgattgct acaaaacgac tatatgcgct
    78001 gtcgatatat gcttgaagca ccgcttcttc ttcttttgtc tctggtcgcg ttcctgaacc
    78061 catatctttc aacgctccgc ttttaactgt tgtatcttcc acacccagtt tttctagaag
    78121 tcctgaataa ttcaatcctg acatgatcac accaattgag ccggtcactg tttcttctgt
    78181 tgcaaagatt ttatctgctt gagcagaaat atagtagcca ccgctagctg ccatgttttt
    78241 catgctgaca tacattggga tatcatgttc tttacgaatc gtatccaatt tcttagcgat
    78301 ttccgcactt tcgtagacac cgccaccagg agaatttact tctaaaaaga tacctttgac
    78361 tgttggatct tcttcaatcg ccttcagttg ttccataaaa tttttatgat tgtaaccttc
    78421 agaagcaaat aaacctgaag accctcccgc agcaatcgtt ccatctactg ataattttac
    78481 gattttttgc gtactgtctc cctcttccaa cacatttgaa gctagttctt catccccata
    78541 taaccaactg ttcaatccct caatttgatc gttttcctga ggcttagtca aactagctga
    78601 aactaacgaa accacaaata atcctgcagc aatcagaact gctgcccaac gtctcttatt
    78661 cattctttct cctttctaat acaagttttt tatgacgatc acagtttctg actgctcaga
    78721 agtgtcgaac cgctctgctt tttcttcccg cgcatacccg gaaaacgact cttttgtcaa
    78781 ttcatcttct accgtaaact cacgagcttc agcaacttcc aaggcatcag gtgcgatttc
    78841 ttggatgcta tcccagccag tatctaaaaa tattttcgta tcatctgata caccagctga
    78901 tgcttcgaat ttggacaatt gtttttttaa cattcccagt gtcatgatct caatttttga
    78961 atccattttt actcctcctc tatccgatta ctttctattg taccgaactc ctgaaaaaga
    79021 tgatagataa aaacccatta ttttttgaca taaaaagagc atttgacaaa gcatttgata
    79081 tataccaatt tattttttat tctttttttg ttgacaagaa cattcgcaaa aggttatgat
    79141 tttaaatgtt tccagataaa aaacaaaaga ggaggatagt tatacctttt aggatagcgc
    79201 caggtctgtg taaacagatg acgaggagag ggtttatcga aagattcggc ggatggccct
    79261 agggactgca ctctacagaa aatccacaaa aaatatctgc aaagataaaa caaaaggatt
    79321 ttcctgcagc tggaagcatt catacgaatt cattatagaa agtaggatgt tttttatgtg
    79381 cggaattgtt ggaatgattg gattaaaaaa tgtaacacca ggattgattg acggtctaga
    79441 aaaattagaa taccgtggtt atgattcagc tggtatcttt gtctcagatg gcacaactga
    79501 ttatctggtc aaagcacaag gcagaattca aaatttgaaa aacaaaataa atattgatac
    79561 tacaggaaat ataggtattg gtcatacacg ctgggcaact cacgggcaac cttctgaaga
    79621 aaacgcccat ccacatactt cccaaagtgg acgcttcgtg ctcgttcaca atggcgtaat
    79681 cgaaaacttt gaagaactta aaattgctta tttagcaaat gaccacttta ttggtgagac
    79741 cgatactgag attatcgctc atctaattga aactttcgcg aaggatacta caacgcaaga
    79801 ggcgttttta aaagctttgc gggtcatcaa aggttcttac gctttcgctt tgattgatcg
    79861 gacagcacct gatgttattt acgtcgctaa aaataaaagt ccactattaa tcggactcgg
    79921 cgatggattc aatgtgattg ccagcgatgc aatggctatg cttgctcata cgaaggaatt
    79981 cgttgagatt gaagatgaag agatggttac cgtcacatca gaaaaagtta ttattcaaaa
    80041 ttttgcaggt gacatcgtag aacgttcttc attcgaagca caagtagatt cttcagatat
    80101 cgagaaaggt acctatcctt tttatatgct gaaagaaatc gacgaacagc ctattatcat
    80161 gcgtcgtatc gctcaaaaat acgtaacaga agacaatcgt gtctctcttg atcaaaagct
    80221 ggtcgataca ttgtctgata gcgatcgaat ctatatcgtt gcttgcggaa ctagttacca
    80281 tgcggggtta gcaggtaaac agactctgga aaaattgaca cagatccctg ttgaggttca
    80341 tttagccagt gaatttggct ataacacacc tcttctttca aaaaaaccat tctttatttt
    80401 cttgagtcaa agtggcgaaa cagcagatag ccgccaagtt ctggtaaaaa tcaaccgtct
    80461 cggctaccct tcacttacga ttacaaatgt tgctggttct actctttcgc gggaagcatc
    80521 ctttacactg ctattgcatg ctggacccga aatcgcagta gcatcaacga aagcttatac
    80581 tgcacaaata gctgtactaa ctttattagc caaagctatc ggtgataaaa aagaatttga
    80641 aacttctctt gtattcgata tcactcatga attgagttta gttgctaatg cgatggaatc
    80701 ggtcatcgcc caaaaagatt atttagaaga gttggcagaa gtatatctca gcgatacaag
    80761 aaatgccttt tacattggac gtggcgctga ctacaacgtt tctcttgaag ctgctttgaa
    80821 attgaaagaa atcagttata tccaggcaga aggttttgcc gctggcgaat tgaaacatgg
    80881 aacgattgct ttgatcgaag aaggaacacc agtgattgga atcatcagtg aagaagtgac
    80941 tggtgcgcat actcgaggaa acctgaaaga agtcgaaagc cgtggcgcta aaacactagt
    81001 tattgtctca gagggattag aaaaggaaag tgaccagctg gtgctaccaa ctgtccaccc
    81061 ttatctaact actctaacca cagtcgtacc gactcaatta cttgcatatt acgcaacgct
    81121 attacgggga ttcgacgttg acaaaccacg taatttagcg aaatcagtga cagtagaata
    81181 ataaaagaac aaggaccagt gtcagacatc tgacactggt ccttgttctt ttatttttta
    81241 tgaaaatatc tcttaatcca gtccgatttc ttctttgact actgccgcga ttttatcgac
    81301 atagtaatta actttctcat cagtaggcgc ttctgccatc actcgaagta atggttctgt
    81361 accggaagga cgaactagga ttcttccttc tgatcccatt tcagcttctg cctcttcaat
    81421 cacttttttg attgccggaa catccatcgc accgttttta tcacttactc ggatattcac
    81481 taatttttgc ggatagattg ttacctctgc tgcaagctct gataatttct tacctgtctg
    81541 tttcatgata ttcagtaact gaattcctga aagcatccca tctccagttg tgttaaaatc
    81601 taagaaaatc atgtgcccag actgctcgcc gccaaagtta taatcgtttt tgcgcatttc
    81661 ttctacaacg taacgatcac caacttgtgt aacgacatct ttcaaaccga ttccttccac
    81721 cgctttgtgg aatcctaagt tactcatgac agtcgtaacg atcgtatctt ttttcaatcg
    81781 atttttttct gccaagtatc tagcacagat aaacatgatt ttatcaccgt cgacaatgtt
    81841 tccaagttca tccaccgcaa taatacgatc gccatcgcca tcgaaagcta gtccagcatc
    81901 tgctcctttt tcaacgacca tctcagctaa acgttctgga tgggtagagc ctacgccatc
    81961 attgatattt aacccgtctg gatttgtgcc catggtataa aaatctgttt caagatcggc
    82021 aaacaaacga ttaacacttg ttgaagttgc tccgtttgct gcatcgacac aaatggtcaa
    82081 accggaaagg tcaccgttga tcgtttgtac aaggaattga gaatatttca ataagccttc
    82141 tggaaattct tctacagttc ccaatccttc agctgatgga cgaggcaatt catccacttc
    82201 agcatctaat aatgcttcaa tttctagctc ttggtcatct actagtttaa aaccatctgc
    82261 accgaaaaat ttgattccat tatcttgtgc tggattatga gaagcagaaa tcatgacccc
    82321 tgcgcttgct ttttgcaaac gtgtcagata agcaacacct ggtgtagaga tcacacctag
    82381 ctggaacact tcgataccca ccgataaaag tcctgaaatc agcgcttctt ctaacatttg
    82441 acctgagatt cttgtatccc gccctactaa cacgcgtggg cgttttcctt cttcttcatg
    82501 ctggcttagg acataacctc cgcaccgtcc cagcttaaat gcaagttctg gcgtaagttc
    82561 cttgttggct tcaccgcgta cgccgtctgt tccaaaatat ttacccatag ttgtaaaatt
    82621 actcctttaa cttaattttc tgaggaattt gttctgctca tttgtgttga ttcgcttgtt
    82681 tctgatgttt cttctgtact tgtggtcgag ctgcttgtcc cagattccga actggaagaa
    82741 atggttgttg tcgatggttc cgttcctgaa tcagcagatg aaatacttct actcgtactt
    82801 gtattttctt gtgtccgact gctcgtcgaa gcagagctac tgcttgacgt ttgcaacacg
    82861 ggtgtaatct ccacactaac tgattcgggc ttgatactta acccctcttc tactggtatt
    82921 tcaattttac gagttgttcg atgattgata cctgagacat tgatcggtac agcaatactg
    82981 tcaccgatcg atgcaagcaa atcgctagag ccgcttactt ctgcttccag ttttgaaaaa
    83041 ctgaaatcat agctttctac cccggcagct ggtgttcctt gctgtatccc ataaagtcgt
    83101 accgacttgc ttggcttgat cgaatcagct ctcactgtta cttgcactgg atcagagacg
    83161 atacttaata cttcccctgc tgcattcagc gcctgtattg ggacctcact attaatccca
    83221 tcatctgtga ccatcgaagc gtccacatca gctactactc gtttgatttc tgccaatgtt
    83281 ttgtcacctg tagtgattga gactttttta ggactaacgg aagtatcatc aagttcatat
    83341 ccagatggtg tgaacgttga aggaatcaac gtttccacat cgaaatcttt tattaccttt
    83401 ttctcaaccg ttactgtaat agtcgatggt tcgatctttg ctgtgacagc actgctcaaa
    83461 ttttgaattt ttagcggaac ctcatgtgtt ccttcgccca gcttagtgat atctgctgtg
    83521 acgcggaaca tccgtgtatc ttcgtttgcc tctgcattca actgaactcg gtttgcactg
    83581 gaaagtttga cagtcactgc attttcatat ccatggatat agtagcgctt tgaatcataa
    83641 gaaatcttga ttggcacccc agtaactgtt tgattatacg cttcatttcc agaaagcgta
    83701 tttggaacgg actggccatt tgcattgaag tacagcgcaa tactgaacag caatgcaata
    83761 aaactgtaaa gaatgcttgt ccgtttctct tttgagatca tttgcgatcc cctcccttag
    83821 tcataccgtc taggaaagat tgaatcacgt ttttcttgtt ttttgttttc tcttcagcaa
    83881 tcagttctct acgcaaaatt gccaaatatt cttgctggga taagccagcc atcagttcat
    83941 tatctaatgt aatgctgaca tccccagttt cttcagagac aacgatggta atcgcgtcgc
    84001 tgacttcgct tataccgaca gctgcccggt gtcgagtccc gaattctttt ggaatcaaca
    84061 tactttctga tagaggaaga taggcgcttg ctactgcgat ctttccttct tttacaataa
    84121 ctgcaccgtc atgcagcggt gtattaggaa taaagatatt gatcagcagc tctccagtga
    84181 tatctgcatc taatgcaatt cctgtctcaa tatactcatc taatccagta tgccgttcga
    84241 tagtaattag cgcaccgatt tttcgcttgg acatatattg gatggctttg tcaaaagaca
    84301 agatcatctt ctcatcttcc tgctgttcgc tttttgattg tttgaaaagc gaactgcgtc
    84361 caagatgttc cagtcctctc cggatttccg gctgaaaaat cacgatagca gctatcaccc
    84421 cgtacgtaat gacttggttc atcagccaag acaaggtgtg cagaccaatg atttccgcta
    84481 cgattcggat aacaataaaa atagctacac ctttcagaag ctgaatcgct tttgtgcctc
    84541 gcagcagttg gatcagtttg tatacaagat accagacaac cagaatatca agtatgttca
    84601 ccaaaaaatt cagagagaac aagtcaccgg aaaacatttg ctgccaatag tccaaattaa
    84661 aaagttcccc gatttgaaat gacataaaaa gcctctcttc tcttttgaga tttcctctct
    84721 agtatatcat atgttttaat tttagaggct gatctgctcg tatttttctt gagaaaaacg
    84781 cataattttc aggaaaattg caagtatgcg ttttattttt ctctgcaata aatattttta
    84841 aacttcagag aattattcat tatatttttt ctgttatttt tccttttttt cttttgtagc
    84901 ttgttccatt tttttacgtc gttctgctct ggcagctctt ctttcttctg cttcaaggtc
    84961 cgtcgttttt tcttttctag tcttaacggg tacatcgata gattcctctg gttcaagtcg
    85021 tttcttgatt ttttctaatt ctcccgtcag tgcagcatag ttgtaaaaac gtgttttagc
    85081 gtcttggata gtcttttcaa ataaagcttt tgcaagattt ggatttgcgt tctctagcga
    85141 ggaaaagcga acttgtttcc tcatgaaatc aggcatagca gagaaatcag gttttttgta
    85201 gtccaaagtc attgggttct tgtttttttc gaccattgca gggttatatc gatataatga
    85261 ccaatagcca gaagaaacag cttcttttgc ttctttaagc gattctttca ttccaccaag
    85321 caaaccatgt gtgatacaag gcgtataggc aatgattaaa gaaggtccag gatacttttc
    85381 tgcttcttca aaagccttta tcgtctgcat ttgattggca cctgaagcaa tttgcgccac
    85441 ataaacattt ccatatgtca tcgccatcat tcctagatct ttctttgaag catactttcc
    85501 actagccgaa aatttagcga ttgccgaagc tggtgttgct tttgaggttt gaccgccagt
    85561 attggaatag acttcgttat ccaaaacaag aatattcacg tctgctccgc tggcaagtac
    85621 atgatcgatc ccgccaaaac caatgtcgta agcccatccg tctccaccaa tcatccattg
    85681 gctgtttttt acgaatagat cctttctctt gtatatatcc tccagttcag ggacaattga
    85741 tcgttcgctt tcgatcgcag cttgcagttt tgctgctctt tgctgcgttc cttcgctctc
    85801 aaacatgtgt accaaccagt cttccatcaa ttctcgtagt tcttctgata cttttggcaa
    85861 tacttctttg attgaacttg ccaaagcctc gcgacgagtc tgtgccgcta gaagcatacc
    85921 ataaccaaat tcagcattat cttccaataa agaatttgac catgcaggcc cttgaccaca
    85981 ttcattggtc gtataaggag aagcaggaga agcaccgccc caaatagaag aacagcctgt
    86041 cgcattcgcg atcatcattc gatcaccgaa catttgtgtc aataatttta cataaggtgt
    86101 ttcgccacag cctgagcaag cgcccgagaa ttcaagaagc ggttgattga attgtgtaga
    86161 caagacagta ttctttcctt tgactggatt tgctttttgt cttaatgtca ttgcaaaagc
    86221 ccaattcaca gcttgttctt tctgttcttc ataaggcttc atcactaaag ctttttcttt
    86281 tgcgggacaa gcctctacac acagaccaca acctgtacag tcttccactg aaacttggat
    86341 acggtatttt acgccgtctg cgcctcgcat ttctctcacg ataaatcctt ctggcgcttc
    86401 ttccatttcc tcttcatctg ctaaaaatgg acggatggca gcatgaggac aaacaaaggc
    86461 acattcatta cacatcgtac agcgatcact aatccactca ggtacttcta aagcgacgcc
    86521 ccgcttttcc atagaagccg ttcctaaagg catttcgcca gttgtcattc catttgctac
    86581 aagtgtacca acagagagtt gatttccttc ctgtgcattc actggttcaa gtatttcttg
    86641 tatataacca gtgttccttt tattgatttt ttcaggcatc tccaacgttc tccaatggtc
    86701 tgggatcgcg acttgatgaa gcagttcggt cgtccgatcg attgcttgta tgtttttttc
    86761 tacgatagcc attgatttat gtccatagct tttcagggct tcttctttta gcagcggaag
    86821 cacctcatca aaaggaatga tgtcagctaa tttaaaaaaa gccgtttcca ttgccgtatt
    86881 gatccggcgt cccaaaccga cttcttgggc caatctcatg gcattgatcg tatagaatct
    86941 aatgttattt tcagccaagt aacgttttaa ttttaagggc agatgcgttt ctaattgttc
    87001 gtcgctccaa agagtattca ataaaaaaat gccacctggc ttcaatcctt tgaccaagtc
    87061 atagctatgc aggtaagctg gtgtgtgaca ggcaacaagg tccgcatgct cgacaagata
    87121 cgctgaacgt atcggcgtat ctccaaaacg aagatgagag acagtcagac cgccagattt
    87181 tttcgaatca taataaaaat agccttgcgc atatttatcg gtgtgatctc caataatctt
    87241 gatagctgat ttatttgctc caactgttcc gtcagatcca aatccccaaa acttggcctg
    87301 gaatgtttgc ggttctgtca aatccagact ttcctttggt tccaaagatt gataagtcac
    87361 atcatccacg atccctatgg taaaccgttt tttgcgtatg cttgggtctt ttttcaattc
    87421 atcaaaaact gctgagattt ggtcgggagt cacatcttta gaccccaatc cgtagcgacc
    87481 accaataaca gccgggcgaa gttcagaatc atataacgca ctttgaacgt ctaataagag
    87541 aggttctcca cctgcccctg gttctttact tcgatcaaga acagcaattg tttttactgt
    87601 atgaggcaac ttttgcacga atgtttccac tggaaaagga cgataaagat ggatattcaa
    87661 aaaacctgtt ttccgtccgt ttgcattcaa gtaatcaatc gtttgttcaa tcgtctgtgc
    87721 aactgatccc atagaaacaa tgacttcttc cgcatcgtct gctccgtaat aagtcaccaa
    87781 gtcataagaa gttcctcgta atcggttgat ctctttcata tatttttgaa cgatttcagg
    87841 taatttatca taataggggt tgatcgtttc ccgttgttga aaatggatgt ctggattctg
    87901 gttcgtacca ctaacagtcg gatgattggg attcatccct cgtttacgaa atgaagctaa
    87961 tttctcctga ttgatcagag gagctaattc ttcatattcc aaaacttcga ttttttgaat
    88021 ctcgtgactc gtacggaatc catcaaagaa attcaaaaaa ggtacttctc cttcaataga
    88081 agcaagatgg gcaacaggtg ctaaatccat cacttcttgt acactgcttt cagctaacat
    88141 agcaaatcct gtttgtcttg cagccatcac gtctccgtga tctccgaaaa tattcaacgc
    88201 atttgtcgtc actgcccgac tagcaacatg gaaaacactt ggcagtagtt ccccagcaat
    88261 cttgtacata ttcggtatca tcaaaagcag cccttgagaa gcggtatatg tagttgtcag
    88321 tgcaccagtt ttcaatgatc catggaccac accagctgct cctgcttcgg attgcatttc
    88381 tactactttt actggttgac cgaatacatt ttcttttcct gctgctgacc attgatctac
    88441 taattcagcc atggtcgaac tgggtgttat tggatatatt gcagcaatct cggtaaatgc
    88501 ataagaaata tatgcggctg ccgtattgcc atccattgtt ttcatttttc gcatgattca
    88561 tcatccttgt tcatattctc tcttattatc tgttccatta taacgtaaaa cattccattt
    88621 tttcttttat ttataaaaat atttcacata tgtctatttg aattggtcat ctaattttta
    88681 gacaaacagt ctaaatcgtt gccttttttt gtcagacatg tataaactat aagcgaaaga
    88741 ggtgttttaa catggatata tcagtgattg atgcaacgaa aacaaataca caaaaaggaa
    88801 tcctttatgg ttcatcgaat gctcctaaaa aaatggtcga attcatcaac ctatcttgtc
    88861 cgtattgccg acaatggttt gaagaatctt atgagctatt agaagaagca gtccaaagcg
    88921 ggcaattgca gcgagtgatc aaattgttcg ataaagaaaa agaaagcttg cagcgaggaa
    88981 acgtgatgca ccggtatctc acaatcagcg atggtcaaaa agcgatcaaa gagatcaaac
    89041 aaatattcga tactcaagac gagtggaaac atctttctct ccaagaagta gcggattttg
    89101 cagtagataa actaaagctg acagaacaaa aagacgaaca gctgagccaa gctgtcatca
    89161 acgaagctga gcaagcacat attcgatttg ttccgactgt catcttgggt aaagaaatct
    89221 ttgatgaatc tatttcaata gaggaattaa aagaattgat ccaaacaaaa taaaaaaata
    89281 aggagcgtag gacaaaagta aagaacactt ttgtctcacg ctctaaacac ctataaacgg
    89341 cggaagcaga agcaactcct tcttatttct tggagttaaa cgcttctgtc ccagcctcct
    89401 tattccgaat aaatggtgtc caaaagcttg catctgccag attatctcat ttcttatgac
    89461 aagctgagca aattatctcc accaaataat ttattcatat ttttgacttg cgcatactaa
    89521 ctaattctta aattttcata caaaatttct caaattccct taacgagtaa tatttatgta
    89581 cggtgattca cactagtatc ccaatcactt tttaagttat cccgatttca attctacttc
    89641 aaaaatagtt gcttttatgt atgctgttag ttgattagat ggtcgtttgt tgagctaggt
    89701 cgtttatttt aaacaagttg tttctttagc ttatacctat ctcgtctacc aaacgcaaga
    89761 tggcaaacac aaatgcatac gcatctgtat ttgccacttt gattcgtata aagctaacaa
    89821 ctcctaactc ctctcattta atagttgctc tcttttttat tcagatacat ttttgctgat
    89881 cacagcctag tatttcaatg gttttgaaca ctaaaccaaa ttgttccatt acaacattca
    89941 ctggctgatg aaatggatta atagatttcg attattcaaa gcgattttct attggtggat
    90001 tgtgttccca actaacaaga cggattccaa agtcattata taactcttca agagttaacc
    90061 ctttcctgtt cccaatagat gtatctctcc cacgctcgat ataaaatatg gagtttcgaa
    90121 tgccaagacc tggcaaatct ttccttgtta cagttacact gctactaact tccccatata
    90181 aaatccagct cctccaaaat gataatatat ttttcgacca tcatctaact catgtaacaa
    90241 taattgttgt gccctcttgt cactcgccaa atgcaaacgc cacgtgcgat accatgccat
    90301 gctacctgca tctgcagaaa atccatcaaa taatgcaaca attgtatctt ctatcacaat
    90361 cctttcccca tctacttcat attctaatct aaaatcaaac tcccctcggg taatcgcagg
    90421 tcttggtggc ttaggaccca atattattct acttaaaacc gcattcgttc ccagatgaaa
    90481 cacagctata acaacaataa tgattaatat tttgtttcta tttgataaat ttctcattat
    90541 tcgcaaatca cctctacatt ctcacatact agttcgtaat actcctttaa tatcaaaata
    90601 tctactgatc gtaataatat tcgctatgaa ttcctactac tcgtataaga aaggccaaat
    90661 catcagcttt tacttgctga tgatttggct tgtttctatt tatcgtaatt gttttctaat
    90721 caccaaaatt gttaacaatc ggttcagata tttcccaatt cactagcctt atattatatt
    90781 gatttaacaa ttcttcttca tttattgtat tttgatcaag cggaccaaat tgttctaata
    90841 attctttgtc atgtaaaata tcccttctag taatgcctct ttccctttca atgaaagaag
    90901 cactaggaaa agtatgatta taaccattca acctatctcc catgtaataa ttagcaggtc
    90961 ctggaaaata aaatatttgc ttattatcac taacttctaa cagcaacact aaatcttctc
    91021 cgctggctaa acgacttgtc catctacgat atctccctat gccttcgttc attccaaatc
    91081 catcaaattc aactattaac gtgtcttcta tcacatgtaa ttcttcattc aattcatact
    91141 caagtctaaa aggaaattct ccatatgtaa tctctggcaa tggtggtgct ggagttctcc
    91201 aaataaatac aataactgtg aatagaaaaa atccaacgat cgatataaat gttatgagac
    91261 ctgctcgaag taaaacccga tataataaaa atgctgtatt tcgctttgct atatctagaa
    91321 aaataaaaag tactgcaatc ataatgatag taaaagttgt aaaaaaacgc atttttgccc
    91381 tccctttata aacatcgatt ctcaaattct aaaccaaatc ttggtagttc aagttcaggt
    91441 ataacgtatt ccaaccatac atctcaatta ctatttatga acttatgatt atcactgaat
    91501 aaatcactcc atccccttgt taaaacttct acattaatta cacctggttg ttttcgacta
    91561 gctaatccaa tgaacttatc aaccatcctt atccaccaac cacgagaatc ttgaccagaa
    91621 gtaagaagag cagttctcaa ggatgatcga gcagttcctt ccagtttttt aattaatcag
    91681 gccagttata gataaatact tgatcttatc tatatcagga cttctatatt aagcaaaaat
    91741 ttatgtacaa aactaaaatc ttcattcagt tgtctccccg ctttttatac gattgtatta
    91801 aaaacaatag aagcctttcc aaattatgtt actccagtaa agatggaatt atagtttgac
    91861 ggaagaaaag caagtataac ttttaaaaaa aactgataca ttcgcatctc ctgcttcatg
    91921 tatcagtttt ttctttggat taaactgctt cttcacgcag tttttccgca aattcattta
    91981 ttttgcgaat tcggtgatta attcccgatt ttgatatcgc acctgaggga atcatttccc
    92041 ctaattcttt caagctgacc tctggatgtt gaagccgaag ttctgcgatt tcctgaagtt
    92101 tttcaggaag ggcatgtaga ccgacctttg cttcgatcaa ttcgatgttt tcgatttgtt
    92161 tagaagccgc atcaatcgtc ttgttcagat ttgctgtctc acaattgact agacgattga
    92221 ccgaatttct catatcccgt acgattcgaa catcttcaaa tcggagcatt gagttggttg
    92281 caccaatcaa tgttaagaaa tcagcaattt tttctgctcc ttttaaataa gaaatatagc
    92341 cattgcgccg ttcaagcgtt cgagcatgta gaccgtaata atttaacata tcgcaaatgt
    92401 cttggttatg ctgttcataa atagaaaata tttccaaatg ataacggctc gtttccggat
    92461 tattgactga acctgaggcc ataaaagcac cacgtaagta agaacgcatt ttttgcgaat
    92521 ttcccataat ctcgtccgaa acgtgtccat tgaacatcat accatccatg atatcaagat
    92581 ccatcaacac atcttttgtg ccttgcttca accggacgat atagacattg ttctttttta
    92641 atttcatttt tttacgaacg agtagctcac ttcgtacctg ataatgatct ttcaaaagag
    92701 aatagatacg tctggcaatt gcagcatttt ctgtttggac gttcaaaata aactgctggt
    92761 tcacaatacc taaagagccg ttcattcgca gcaaagcagc taattccgct ttcgcatgtt
    92821 ctcgatgaac ttccaaacca gttagttctt tcttcacatc tgcagcaaat gacatcctat
    92881 tctcctcctt tctccaacaa taaattgctt aatattttga accgaaaact aaacggaaca
    92941 attcttctac tactttgtct ccatcgtgga agacaccacc atctttcaat tccaaaaagt
    93001 cggtggaaat cactcgacat ccttcatttc tcaatccgtt gaagtcgtgt gcgacttgta
    93061 ctagatactc atcatagatc tctgggtcca tataatcttc aggcactttt tccgtattga
    93121 ccaatactgt atcgataaaa ggctgattca aatgcttgtg taaaacacgt atatgatcgg
    93181 catctgtgaa atgttccgtt tccccttttt gggtcatgat attacagata tagacggttt
    93241 cagcagttgt cttgagcatt gcttcgccga tttctggtat cactaaattt ggaaggatac
    93301 tggtgaataa acttccgggt cccaaaacga ccatgtcggc gtccataatt gctgaaacga
    93361 cttttcgagc cgctttggct tcacttttat catttgtgtt gcagacatac acacggtcaa
    93421 tggttttacg gtcgatcgcg attttagatt ctcctactgc agtcgttccg tctttaaaga
    93481 cagcatgcaa ggtcaaagga gtttctgaag aaggataaac atgtccatct acatgcatca
    93541 tttttgctaa taattgaatc gcctcataag tgcttccccg catctcggaa actgcggcaa
    93601 tgatcagatt accgatcgta tgattggcta aaaactgatc ttcctttttg aaacggtatt
    93661 ggaaaatatc ttcatagaat tgtggcatat ccgataacgc cactaagaca tttctcaagt
    93721 caccaggagg agccatagcg atcgattctc tgattgcacc gctgcttccc ccatcatctg
    93781 caacagtcac tactgctgtg atatctgctc cttgatttcg caaacttttc aggataactg
    93841 gcagaccagt gccaccgccg accactacga tctttggttt tcttatccgg taggttttca
    93901 ttttcacgac cggttcaccg tctctttcct tttatccttg tcccgatgtg tcacattgac
    93961 tttatagttt ttccctaaag catttgccaa acgttcagtc aatgccacag aacggtgttg
    94021 accgcctgta cagccgattg caactgtcaa gctgcttttt ccttctttaa tgtatccagg
    94081 caatacagtt tccagaagat ccaaaaattt atggtagaat gattgcgttt catcaaagga
    94141 catcacataa tcatagactg gctgatcttt tccggtcaat ggtcttagtt ccgggatata
    94201 atgagggttg ggcaaaaaac gcacatccat cacaatatcc gaatcgatcg gcaacccgta
    94261 tttaaaacca aatgatacaa attcgatccg aaatccttgg tctccctttg atttgaatga
    94321 ctcattgatt ttttcacgta attgtcgtgg tgtcaatgtc gtcgtatcaa tgatcacgga
    94381 agcttgggct ttcaagtcgt ctagtatcgc ccgttcttta cgtatccctt cagtgatcaa
    94441 accgtccatc gccattggat gtgctcgtct tgtttctttg tagcgagcca ctagttcttc
    94501 gtctgaagca tctaaaaata agatagaagt gtcgatgaag ttcgtatttt cgatttcgat
    94561 cagcatgttt tggatttctt caaagaagga acgagaacgt aaatccactc ctaaagccat
    94621 ttttgtgact ttgcctgatt ctttgattaa ttcccagaat tttggaatca atcgaggcgg
    94681 catattatcg atacaaaaat agcccatatc ttcaaaactt tggatagcta ctgttttccc
    94741 tgcaccgctc atacctgtta tgatgactaa ttgcaaatta tctgtcataa ttacgaactc
    94801 ctctcacatt gtgcatagta aaattatatc attccgggcg tatcattacc agttttcttt
    94861 ttcttttaca aaaagatttg gaccaaatat cttttaggat atttgcccca aatccaattt
    94921 ttttactttt ccgatgctgt atccaatgcc ttgcttcttt cagctagcgc tcgttcaatc
    94981 gtccgatctg ctaaaataga ttgggcatcg atcactggat aatggttatc ttcagcaaat
    95041 tcatgcatca atgacaattc tgtgcaacct aagataactt tttcacagtt taggcgctct
    95101 accgcttctt ctaagatttc gtaatatagc tcttgattca aataatccga ttccttgatc
    95161 tcatgataga tcagataatt gattttttct tgaagaacag tatcaggaat cactgtttcg
    95221 aatccaagat ttttgacttc gcgttcatag attccagctt tcatacttcc ttctgttcct
    95281 agaatcgcta ctctccctgt cgtatgctga cggacaagtt catttgctgc ttctcttggc
    95341 atatgaagga taggaatatc cgttgccgct tgtaattctt caaaaaagta gtgtgcggtg
    95401 ttacaagtca acacaatgaa gttcggctgc aataaatttt gtttctcaat atcatccagc
    95461 agaaacggca ttggattttc ttctgatcga tctaaaatat aagctgtacg atcaggaact
    95521 gttgcatgat tgaataagac ataattcaaa tattcttgat cttttgtagc tttcgttcgg
    95581 tggttgatca agcgaacaaa actttctgtc gccatcgtgc ccattccgcc taaaatactg
    95641 aaaaaattct ccatgaacta gccttctttc acatgaaaat actttttgaa tcttggaata
    95701 tagttatgga acatatattt catcaacaac cagcgcttga ttgaccggtc tttttcataa
    95761 aacacagtag taccgtaacg attttctttg atcatttgct cagctagttt tttgtcttca
    95821 ttttcccgag catattctaa aaagattttt ttgggcacac ccatccataa tcttgcttta
    95881 tctgattgat tcgttccata ggtcgtttca acaaagggtt tgttaaacac tcgatcttct
    95941 gtgacgaaac gagccagatt caagccgttc aatgtcacaa agaaactgct acgaccttgt
    96001 ctcaaattga tttcaaataa tttatattct ccatctctag gatcatactt catatcgaaa
    96061 ttggcaaagc ctgtatattc gattttttcc aaaaatgcct taatggtttg atagattttt
    96121 tcgttataat ctggcatgat cacaacgtaa ttaccaatcg atgctggtgt aggatcttct
    96181 aacagtggat gaccaagaca catcatccga acttgatgat cttcatctac atacgcattt
    96241 agcacacgca tattactatc atcacccggg ataaaatctt gtacaatcat ttcactttta
    96301 taaccggctt cgtatatccg gcctaaaatt aaatcgaact cttctctagt ctctaaaata
    96361 aaagcttttt ttcttccttc aaattggaca gaaagatact ccacactgtt tgcaggtttc
    96421 aaagcaactg ggaattcaaa aggcaattcc tgttccaaat gtccatttac taacatttct
    96481 tctctgacga tcaatgtttt aggatacgga agatcatatt cttcacaaac ttcataaaag
    96541 cttactttat tgatcagtct ttcaaataaa gaataatcaa tatatggaca gataaaatat
    96601 tctgacagct cttgtttatg ttgggagatc aattctgcat atccatcacc acaagcaatc
    96661 aaaagatatt ttttactctt atcagaatat ctttcttttg ccaaacgaag catcgtctcg
    96721 ataaacacag ggtctttatc aaatcctgga ataacttcta cattgacaat tttactgtag
    96781 cgggtagggg ctaactgatc cgaagcatat gcttggcaga ttttgccgta tgcttcatta
    96841 aaagaacgtg ccataccata aacgttcatg tcgcttccta ataatatagg gataaattct
    96901 tcattttcaa tactgttcat cattatctct cacttactca taatctcttg tttttctttt
    96961 ccaactacac aaggaatatc ataccataaa gaaaaaagca atcaaaggaa actcctccga
    97021 ttgctcaatt cttttttgaa cggattgaac ttataggaag tttttgtcta taccaatcag
    97081 tgtagaaaga actaccatgt aatgattcta gaacactgat attaataaaa caagcaaaaa
    97141 ttcatttttt tatgtttttt taactatctc agctatctat cctctttata ccattatttt
    97201 tttcaaatag tgacctgtat aactttcttt tacgttaatg atttcttctg gtgttccagt
    97261 cgcaaggatc gttcctccac cttctccacc ttctggtcct aaatcaatga tataatctgc
    97321 tgttttgatt acatctagat tgtgttcaat cactaaaact gtgttaccag catctactaa
    97381 tctttgtaat acatgcaaca agcgggcgat gtcatcgcta tgaagtcctg tcgttggttc
    97441 atctagtata tagaaattct ttccattaga gattttgtgc aattcactgg caagtttcat
    97501 ccgttgtgcc tcaccaccgg acaatgtcgt tgctggttgc cccatcgtca cataacctaa
    97561 gccaacatca acaatcgttt gcagtttgcg atgaatcttt ggaatgtgct tgaagaattc
    97621 tacagcatct tctactgtca tttccaaaat atcagcaatg ctttttcctt tgtaatgcac
    97681 ttctaatgtt tcagagttat atcgtttgcc atgacatact tcacaaggaa catagacatc
    97741 aggcaaaaag tgcatttcga tcttaataat tccatccccg cgacaagctt cacaacgacc
    97801 gccttttacg ttgaaactaa aacgcccttt cttataaccc cgcatttttg cctcgttcgt
    97861 ttgagcaaat aacccacgga tatcatcaaa tacacttgta taagtcgctg gattacttct
    97921 cggcgtccgg ccgattgggc tttgatcgat atcgatgatc ttttcgatac tttcgtagcc
    97981 ggaaattgtc ttgaatttac ctggcttagc agaattctta tttaattttt gcgctaacga
    98041 tttctttaag atactattga ccaatgtact ctttccagaa ccagacacac ctgtgactgc
    98101 tacaaattct cctaatggaa attcaacact aatatttttc agattgtttt ctgaagcacc
    98161 agtgatcttg attgctttcc cattaccttt acggcgttct tttggtacag ggattgattt
    98221 tttccctgaa agatatttcc ctgtcaaaga attttcatca ttagctactt cttcaggagt
    98281 gcctgcagcc acgatttgtc cgccttgttc tccggcccca ggtccgacgt caatcagata
    98341 atctgctgcc cacatcgtat cttcatcatg ttcgactaca acgagggtgt tgccaagatc
    98401 acgcattttc tttaatgagc caatcagtcg atcgttgtcg cgttgatgca atccgatcga
    98461 tggttcatct aaaatataca aaactccaga aagattcgat ccaatttgtg tagccaatct
    98521 gattcgctgc gcttctcccc cagataatgt tccggaagcg cgactcaaag ttagatagtc
    98581 tagtcccaca ttttccaaga atgaaagtct gtcatttact tcttttaaaa tcggtttggc
    98641 aattactttc tcttgttcag ataacgttaa gtttgagaag aaatctactg ctttttgaat
    98701 agacaactca tttgtttgcc cgatattcgt tccattgatt tgaacagata gagcttgggg
    98761 attcaatcga taacctttac atgtctgaca agttaattct gtcatataaa gacgcatttg
    98821 ctcacgcgtg aaatcactgt tcgtatcatg ataacgacgc tcgatattct tcaatacccc
    98881 ttcgaaagtc gtttctacat cgcgaacgcc accaaaatca ttttcataat ggaaatggaa
    98941 aagatcatct gtcccattca agatgatatc ctgatgctct tccggtaatt cttcgaatgg
    99001 tgtatctaaa tcgataccga aactttcaca agcctgcgct aacatttgag gataatactg
    99061 agagctgatt ggattccatg gaacgatagc accttgacta agtgtcttcg ttctatctgg
    99121 aatcactaaa tcttgatcga cttctaattt gacacctaaa ccatcacagt ctggacaagc
    99181 tccaaaaggc gcattgaatg agaacagtct tggttctagt tcgccaacag taaatccgca
    99241 ataaggacag gcatagtgtt cgctaaacaa catttcttct tcaccgatca cgtcaaccag
    99301 agcatagccg tctgccaatc gcagagccgc ctctaaagaa tcaaatagac gagaacgaat
    99361 cccttctttc acaatgatcc ggtcgatgac gatcgcgata tcatgttttt tgtttttctc
    99421 cagctcaggc acttcactta aatcatagat ctcaccatcg actcgaacgc ggacgtatcc
    99481 ctctttttgg attcgttcga atattttttt atgttgtcct tttttcttta ccacgatcgg
    99541 tgccaacact tggatctttg tgcgctcagg caaagccatt acttgatctg ccatttgatc
    99601 tactgattga ctggtaatct cgatatgatc gttgggacaa atcggatgtc ctacacgtgc
    99661 aaacaacaag cgtaaatagt cattgatttc cgtcacagta cctactgtcg accgaggatt
    99721 tttacttgtt gttttctgat caatagaaat cgccggactc aatccgtcga tgctatctac
    99781 gtccggctta tccatttgtc ctaaaaactg acgagcataa gcagaaaggc tctctacgta
    99841 tctccgctgc ccttcagcgt acagcgtatc aaatgctagg gaactttttc cagaaccgga
    99901 caaacctgta acgaccacga atttatctcg tggaatcgtt acatctatat ttttcaagtt
    99961 atgggcacgt gcaccgtgaa tcgtaatttt atcgttagcc atgttgtcct cctactttga
   100021 agcttttaat tctaaaatca tatctcgcac attagcggct gtttcaaaat ccaatgcttt
   100081 cgccgcattt ttcatttctt tttctaattt catcaataac gtatcttttt cttctttcgt
   100141 catttcttca tacgagactt gtaccgcttc ttttgtatca tcttccgatt ctttcgtaat
   100201 agaaatcaaa tctcggattt ctttaatgat cgtttttgga acgatcccat gttcttcatt
   100261 gtacttctgc tgaatcgtac ggcgtcttga tgtttcatcc atcgctagac gcatagaatc
   100321 tgttactttg tctgcgtaca tgattacttt accttcttca ttccgagcag ctcgtccaat
   100381 cgtttggact aaggaacgtt cactacgtaa aaagccttct ttatccgcat ccaagatagc
   100441 aacaagggaa acttcaggta cgtctaatcc ttcacgaagc aaattgattc caactaacac
   100501 atcaaattca cctaatcgaa gatctcgaat gatttctgtt cgttccagcg ttttgatatc
   100561 actatgcaaa tatttgactt tgatccctaa ttctttgaaa taatctgtga ggtcttctgc
   100621 catttttttc gtcaatgtcg ttacaaacac ccgctggtct ttttcgactc gttcatttat
   100681 ttcgcctact agatcatcga tttgtcccat gattggacga acttcgatca ctgggtcaag
   100741 cagccctgtc ggacggatga tttgttggat aacggtatct gtttgttcat gttcatatgg
   100801 tcctggcgtc gcagagacat aaatgatctg attgacatgt ttttcaaact cttctaaccg
   100861 aagtggccga ttgtctaaag cagatggcaa acggaaaccg taatctacta gcatctgttt
   100921 tctcgctcgg tcaccattat acattcctct gacttgaggc atggttacgt gcgattcgtc
   100981 cgccacgatc aaaaaatctt caggaaagaa atccaatagc gtataaggcg gttcaccttc
   101041 tttacgtccg tccatatgcc gagaataatt ttcaattccc gacgtgtagc ccatttctaa
   101101 catcatttca atatcgtaat tggttcgctg ttctaaacgt tgagcttcca gaagtttgtt
   101161 ttcatttcgc aagacagtta aacgctgttc taattccgct ttgatattcg ctacggcatg
   101221 ctccatatgt tcatcattcg tcacaaagtg agtcgccggg aagattgcca cgtgttccgt
   101281 ttctcctaaa acttcacctg tcagtgcatt gacttctcgg atacgttcga cctcatctcc
   101341 aaaaaactcc acacgtaagg cacgttcatc tcttgaagcc ggaaaaatct ccacgacatc
   101401 accgcgcact cgaaaacgac cgcgctgaaa atctatatca tttctttcga attggatact
   101461 gactaagtca cgaatcaact gattgcgatc tagttctgct ccctgtcgaa tcgaaactac
   101521 ttgtttttgg tactcaaaag gcgaacctaa tccaaaaata catgaaacgg atgcaatgac
   101581 gatcacatca tttctttcta ataatgaact agtagccgaa tgtcgtaatt tgtcgatctc
   101641 atcatttaca cttgaatctt tttcgatata cgtatcgctt gaaggcacat aagcttcagg
   101701 ctgataataa tcataataag aaacaaaata ttcaacagca ttgttaggga aaaattcctt
   101761 gaattccccg tacagctgac cagccaatgt tttgttatgg gcaataatta gtgttggttt
   101821 attcactttc tcaataagat tggaaatcgt atatgttttc cctgttccag tcgcacctaa
   101881 taagatttgt gcttttttcc cgccgactac gccatccact aattggttaa tcgcttcagg
   101941 ctgatcacca gccggttggt atttcgagac aagctcaaag tgtcgggagg tatctctctc
   102001 aatcattctt cgtcctcctt ttcattcatt ccttttcatt ttacgaccaa taaaaagaaa
   102061 aggaaagcaa aggcattcct tactaaaaaa ttataccaca ccgaacatac tttcgccact
   102121 tttttatctt attatgcatg ttacgtttta taacataaaa tgttagaaac tcgcattttt
   102181 ttcatttttt cgagcttaca gtcttgtctg attcagcact tttcgcctat aataataaaa
   102241 tattggaaat ttaggaggtt gattatgaaa agaaagcatt tatttcactc atttgttttc
   102301 ttaatgacag gacttgtgac acttgcgttt ggacatgttg cacaagctga caacactaca
   102361 acggcagagt caagcacaag cgagacaatg caaaaaatag agcccaaaaa agacgtatac
   102421 gtaattgcca gtgactctgc ttttgctcca tttgaatttc aaaacgcaaa gggcgagtac
   102481 gaaggcatcg atgtaaaatt gatggaaaac atcgccaaac tgcaaggatt taacatccga
   102541 atggactacc gtggtttctc tgctgctttg caagctttgg aatcaggaca agcagacgga
   102601 atgattgccg gaatgactgt aacagatgaa cgaaagaaaa gctatgattt ttctgattct
   102661 tactttgata gcgggatcca attagctgtc aaaaaaggag atacttcgat caagtcttat
   102721 gaagatttga aaggaaaaac agtcggagca aaagttggta ccgaaagtgc tgaattttta
   102781 agcgaccatg aatctgaata tggctattct atcaaatatt ttgatgctgc tgatcaattg
   102841 tacgacgggt taaaagttgg cgcaatcgat gcaatgatgg atgattatcc ggtaatcggt
   102901 tatgcgatca aacaaggaca agaattggaa acaccgatca aaaaagaatc tggcggaaaa
   102961 tatgcatttg ccgttaaaaa aggacaaaat cctgagcttc ttaatatgtt caatgaagga
   103021 ttgaaagaat taaaaagaac aggcgaatac gataagatta ttaaagaata tgtagcggat
   103081 ggttcagaaa cttccagtga gtcttccact gctgatgaat caacatttct tggacttatc
   103141 caaaacaact atcaagcatt gttgaaagga ctttggagaa caattctcct aactttgatc
   103201 tcctttgctt tggctatagt aatcggtgtg atttttggat tattcagtgt agcaccaata
   103261 cgcgctcttc gcacaattgc tagtatttat gtcgatatca tccgaggaat tccaatgatg
   103321 gttcttgcgt tctttatttt ctttgggttg cctggtatcg ttggatttac gattcctgac
   103381 ttccttgctg gtgttatcac cttgactctt aacgcaagtg cttatatcgc agagattgtt
   103441 cgaggcggga tcaacgccgt tcctgtcgga caaatggaag cttccagaag tttaggtctt
   103501 tcttataatc ggacaatgca aaaaatcatt ttgccgcaag cgatacgcat catgatccca
   103561 tcattcgtca atcagttcgt tatctcatta aaagacacaa cgatcatttc cgcaatcggt
   103621 gtagtcgaat tgctgcaaac tggtaagatc attgttgcac gtacaacaca aagtacgtat
   103681 gtttacttga tcattgcgat catgtacttg attttgatca cagcattgac aaaattagca
   103741 aaagttttag aaaagaaggt gaagtaagat ggcagaaaaa atcttagttg agcatttagt
   103801 caaaaaatac ggcgataaca cagtattaaa cgacatcaat gtgtccatca acgaaggaga
   103861 tgtcgtctgt atcattggcc cttcaggttc aggaaaaagt acatttttgc gttgcttgaa
   103921 tcgtttagaa gaaccttctg acggtgaaat tattattgac ggcgcccact tgatggataa
   103981 aagcacagat atcaacttgg tgagacaaca tatcggaatg gtattccagc actttaacct
   104041 attccctcat ctttctattt tggaaaacat cattttagca ccgatggacc tgaagaaaga
   104101 atctagagaa gaagcagaga aaaaagcaat caaattgctg cagacagttg gtctagaaga
   104161 taaaaaagat atgtatcctg aaatgctttc cggtggacaa aagcagcgtg tggctattgc
   104221 tcgtgctcta gcgatgaacc ctgacatcat gctttttgat gagcctacat ctgcactcga
   104281 ccccgaaatg gtcggtgatg tcttgaacgt catgaaaaag ttagcagaac aaggtatgac
   104341 gatggttatc gttacgcacg aaatgggctt cgctaaagaa gtggccaatc gtgtcatgtt
   104401 cattgacgac ggaaactttt tagaagacgg aacgcctcaa caaatctttg aaaatccgca
   104461 aaatgaacgg acaaaagatt tcttgaataa agtattaaat atttaataaa aaccaaaaat
   104521 gcccggtcgc ttcatgacga ccgggcattt ttagtttctt gttttttcac ttaactgtac
   104581 cagccgcatc gcttgttctc ctgcacaaac aagcaatcgc tctacctgtt ccatcgcata
   104641 agaaagtacc atcggttcat tgacgatatc aagtacatag tcgataccac cttcagaaac
   104701 tatgatgatt atcttattct aaagctggct tttttgttgg tacaaacatg acaactacaa
   104761 aaaa
//
LOCUS       DANJMI010000003        92825 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000003 DANJMI010000000
VERSION     DANJMI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 92825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 92825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.003"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(7..1581)
                     /locus_tag="PWU81_000247"
     CDS             complement(7..1581)
                     /locus_tag="PWU81_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL1108985.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(1836..2345)
                     /gene="mreD"
                     /locus_tag="PWU81_000248"
     CDS             complement(1836..2345)
                     /gene="mreD"
                     /locus_tag="PWU81_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL1108986.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(2349..3203)
                     /gene="mreC"
                     /locus_tag="PWU81_000249"
     CDS             complement(2349..3203)
                     /gene="mreC"
                     /locus_tag="PWU81_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL1108987.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(3358..3996)
                     /locus_tag="PWU81_000250"
     CDS             complement(3358..3996)
                     /locus_tag="PWU81_000250"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDL1108988.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(3997..4647)
                     /locus_tag="PWU81_000251"
     CDS             complement(3997..4647)
                     /locus_tag="PWU81_000251"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL1108989.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(4660..5559)
                     /gene="rsgA"
                     /locus_tag="PWU81_000252"
     CDS             complement(4660..5559)
                     /gene="rsgA"
                     /locus_tag="PWU81_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDL1108990.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(5724..7793)
                     /gene="pknB"
                     /locus_tag="PWU81_000253"
     CDS             complement(5724..7793)
                     /gene="pknB"
                     /locus_tag="PWU81_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDL1108991.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(7790..8530)
                     /locus_tag="PWU81_000254"
     CDS             complement(7790..8530)
                     /locus_tag="PWU81_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDL1108992.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(8543..9901)
                     /gene="rsmB"
                     /locus_tag="PWU81_000255"
     CDS             complement(8543..9901)
                     /gene="rsmB"
                     /locus_tag="PWU81_000255"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL1108993.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(9901..10839)
                     /locus_tag="PWU81_000256"
     CDS             complement(9901..10839)
                     /locus_tag="PWU81_000256"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL1108994.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(10832..11323)
                     /gene="def"
                     /locus_tag="PWU81_000257"
     CDS             complement(10832..11323)
                     /gene="def"
                     /locus_tag="PWU81_000257"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL1108995.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(11338..13746)
                     /gene="priA"
                     /locus_tag="PWU81_000258"
     CDS             complement(11338..13746)
                     /gene="priA"
                     /locus_tag="PWU81_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL1108996.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(13999..14304)
                     /locus_tag="PWU81_000259"
     CDS             complement(13999..14304)
                     /locus_tag="PWU81_000259"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL1108997.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(14309..14923)
                     /gene="gmk"
                     /locus_tag="PWU81_000260"
     CDS             complement(14309..14923)
                     /gene="gmk"
                     /locus_tag="PWU81_000260"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL1108998.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(15410..15694)
                     /locus_tag="PWU81_000261"
     CDS             complement(15410..15694)
                     /locus_tag="PWU81_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1108999.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(15712..16602)
                     /locus_tag="PWU81_000262"
     CDS             complement(15712..16602)
                     /locus_tag="PWU81_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL1109000.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(16622..16840)
                     /locus_tag="PWU81_000263"
     CDS             complement(16622..16840)
                     /locus_tag="PWU81_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HDL1109001.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(16810..18510)
                     /locus_tag="PWU81_000264"
     CDS             complement(16810..18510)
                     /locus_tag="PWU81_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL1109002.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGRGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            18777..19550
                     /locus_tag="PWU81_000265"
     CDS             18777..19550
                     /locus_tag="PWU81_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109003.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(19631..20200)
                     /gene="thiT"
                     /locus_tag="PWU81_000266"
     CDS             complement(19631..20200)
                     /gene="thiT"
                     /locus_tag="PWU81_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HDL1109004.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(20266..20368)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(20374..21345)
                     /locus_tag="PWU81_000267"
     CDS             complement(20374..21345)
                     /locus_tag="PWU81_000267"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL1109005.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(21439..22812)
                     /gene="glmU"
                     /locus_tag="PWU81_000268"
     CDS             complement(21439..22812)
                     /gene="glmU"
                     /locus_tag="PWU81_000268"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL1109006.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(23315..24133)
                     /gene="purR"
                     /locus_tag="PWU81_000269"
     CDS             complement(23315..24133)
                     /gene="purR"
                     /locus_tag="PWU81_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDL1109007.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(24300..25124)
                     /locus_tag="PWU81_000270"
     CDS             complement(24300..25124)
                     /locus_tag="PWU81_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL1109008.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(25097..25777)
                     /locus_tag="PWU81_000271"
     CDS             complement(25097..25777)
                     /locus_tag="PWU81_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL1109009.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(25800..26738)
                     /locus_tag="PWU81_000272"
     CDS             complement(25800..26738)
                     /locus_tag="PWU81_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDL1109010.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(26983..27846)
                     /locus_tag="PWU81_000273"
     CDS             complement(26983..27846)
                     /locus_tag="PWU81_000273"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL1109011.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(27988..30561)
                     /gene="mprF"
                     /locus_tag="PWU81_000274"
     CDS             complement(27988..30561)
                     /gene="mprF"
                     /locus_tag="PWU81_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL1109012.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            30702..31508
                     /gene="proC"
                     /locus_tag="PWU81_000275"
     CDS             30702..31508
                     /gene="proC"
                     /locus_tag="PWU81_000275"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL1109013.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(31587..33143)
                     /gene="rny"
                     /locus_tag="PWU81_000276"
     CDS             complement(31587..33143)
                     /gene="rny"
                     /locus_tag="PWU81_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDL1109014.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(33554..34603)
                     /gene="recA"
                     /locus_tag="PWU81_000277"
     CDS             complement(33554..34603)
                     /gene="recA"
                     /locus_tag="PWU81_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL1109015.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(34712..35953)
                     /locus_tag="PWU81_000278"
     CDS             complement(34712..35953)
                     /locus_tag="PWU81_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDL1109016.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(36193..36771)
                     /gene="pgsA"
                     /locus_tag="PWU81_000279"
     CDS             complement(36193..36771)
                     /gene="pgsA"
                     /locus_tag="PWU81_000279"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL1109017.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(36980..37903)
                     /locus_tag="PWU81_000280"
     CDS             complement(36980..37903)
                     /locus_tag="PWU81_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1109018.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(38642..39928)
                     /locus_tag="PWU81_000281"
     CDS             complement(38642..39928)
                     /locus_tag="PWU81_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL1109019.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(39921..41189)
                     /locus_tag="PWU81_000282"
     CDS             complement(39921..41189)
                     /locus_tag="PWU81_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL1109020.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            41441..41902
                     /gene="mscL"
                     /locus_tag="PWU81_000283"
     CDS             41441..41902
                     /gene="mscL"
                     /locus_tag="PWU81_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL1109021.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(41961..42407)
                     /locus_tag="PWU81_000284"
     CDS             complement(41961..42407)
                     /locus_tag="PWU81_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1109022.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(42500..42745)
                     /locus_tag="PWU81_000285"
     CDS             complement(42500..42745)
                     /locus_tag="PWU81_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDL1109023.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(42860..43438)
                     /locus_tag="PWU81_000286"
     CDS             complement(42860..43438)
                     /locus_tag="PWU81_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL1109024.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(43515..44045)
                     /locus_tag="PWU81_000287"
     CDS             complement(43515..44045)
                     /locus_tag="PWU81_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDL1109025.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(44049..44909)
                     /gene="rlmB"
                     /locus_tag="PWU81_000288"
     CDS             complement(44049..44909)
                     /gene="rlmB"
                     /locus_tag="PWU81_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL1109026.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(44902..45297)
                     /locus_tag="PWU81_000289"
     CDS             complement(44902..45297)
                     /locus_tag="PWU81_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDL1109027.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(45294..46706)
                     /locus_tag="PWU81_000290"
     CDS             complement(45294..46706)
                     /locus_tag="PWU81_000290"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL1109028.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(46703..47239)
                     /gene="cysE"
                     /locus_tag="PWU81_000291"
     CDS             complement(46703..47239)
                     /gene="cysE"
                     /locus_tag="PWU81_000291"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL1109029.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(47325..47564)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(47561..49021)
                     /locus_tag="PWU81_000292"
     CDS             complement(47561..49021)
                     /locus_tag="PWU81_000292"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL1109030.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(49191..50348)
                     /locus_tag="PWU81_000293"
     CDS             complement(49191..50348)
                     /locus_tag="PWU81_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDL1109031.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(50382..51752)
                     /gene="radA"
                     /locus_tag="PWU81_000294"
     CDS             complement(50382..51752)
                     /gene="radA"
                     /locus_tag="PWU81_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL1109032.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(51815..52342)
                     /locus_tag="PWU81_000295"
     CDS             complement(51815..52342)
                     /locus_tag="PWU81_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL1109033.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            52572..53378
                     /gene="proB"
                     /locus_tag="PWU81_000296"
     CDS             52572..53378
                     /gene="proB"
                     /locus_tag="PWU81_000296"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL1109034.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            53375..54622
                     /locus_tag="PWU81_000297"
     CDS             53375..54622
                     /locus_tag="PWU81_000297"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL1109035.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            54698..55042
                     /locus_tag="PWU81_000298"
     CDS             54698..55042
                     /locus_tag="PWU81_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109036.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(55159..55485)
                     /locus_tag="PWU81_000299"
     CDS             complement(55159..55485)
                     /locus_tag="PWU81_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109037.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(56100..57302)
                     /locus_tag="PWU81_000300"
     CDS             complement(56100..57302)
                     /locus_tag="PWU81_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1109038.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            57419..57934
                     /locus_tag="PWU81_000301"
     CDS             57419..57934
                     /locus_tag="PWU81_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL1109039.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(58133..58579)
                     /locus_tag="PWU81_000302"
     CDS             complement(58133..58579)
                     /locus_tag="PWU81_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL1109040.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(58626..60104)
                     /gene="msr(C)"
                     /locus_tag="PWU81_000303"
     CDS             complement(58626..60104)
                     /gene="msr(C)"
                     /locus_tag="PWU81_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL1109041.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(60458..61087)
                     /locus_tag="PWU81_000304"
     CDS             complement(60458..61087)
                     /locus_tag="PWU81_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL1109042.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(61071..61535)
                     /locus_tag="PWU81_000305"
     CDS             complement(61071..61535)
                     /locus_tag="PWU81_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL1109043.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(62022..63290)
                     /locus_tag="PWU81_000306"
     CDS             complement(62022..63290)
                     /locus_tag="PWU81_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL1109044.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(63521..64258)
                     /locus_tag="PWU81_000307"
     CDS             complement(63521..64258)
                     /locus_tag="PWU81_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1109045.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(64325..64894)
                     /locus_tag="PWU81_000308"
     CDS             complement(64325..64894)
                     /locus_tag="PWU81_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL1109046.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            65022..65894
                     /locus_tag="PWU81_000309"
     CDS             65022..65894
                     /locus_tag="PWU81_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL1109047.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            66236..67108
                     /locus_tag="PWU81_000310"
     CDS             66236..67108
                     /locus_tag="PWU81_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL1109048.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            67105..69063
                     /locus_tag="PWU81_000311"
     CDS             67105..69063
                     /locus_tag="PWU81_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1109049.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(69395..70246)
                     /locus_tag="PWU81_000312"
     CDS             complement(69395..70246)
                     /locus_tag="PWU81_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1109050.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            70369..70836
                     /locus_tag="PWU81_000313"
     CDS             70369..70836
                     /locus_tag="PWU81_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDL1109051.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(71099..71581)
                     /locus_tag="PWU81_000314"
     CDS             complement(71099..71581)
                     /locus_tag="PWU81_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDL1109052.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(71681..72544)
                     /locus_tag="PWU81_000315"
     CDS             complement(71681..72544)
                     /locus_tag="PWU81_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL1109053.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            73142..74017
                     /locus_tag="PWU81_000316"
     CDS             73142..74017
                     /locus_tag="PWU81_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109054.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(74162..74314)
                     /locus_tag="PWU81_000317"
                     /pseudo
     CDS             complement(74162..74314)
                     /locus_tag="PWU81_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(74582..75913)
                     /locus_tag="PWU81_000318"
     CDS             complement(74582..75913)
                     /locus_tag="PWU81_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1109055.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(76062..76775)
                     /locus_tag="PWU81_000319"
     CDS             complement(76062..76775)
                     /locus_tag="PWU81_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1109056.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            76950..78374
                     /locus_tag="PWU81_000320"
     CDS             76950..78374
                     /locus_tag="PWU81_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL1109057.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            78673..79380
                     /locus_tag="PWU81_000321"
     CDS             78673..79380
                     /locus_tag="PWU81_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL1109058.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            79367..79690
                     /locus_tag="PWU81_000322"
     CDS             79367..79690
                     /locus_tag="PWU81_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL1109059.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(79779..80816)
                     /locus_tag="PWU81_000323"
     CDS             complement(79779..80816)
                     /locus_tag="PWU81_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL1109060.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(81137..82120)
                     /locus_tag="PWU81_000324"
     CDS             complement(81137..82120)
                     /locus_tag="PWU81_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDL1109061.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(82535..83305)
                     /locus_tag="PWU81_000325"
     CDS             complement(82535..83305)
                     /locus_tag="PWU81_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1109062.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(83298..84233)
                     /locus_tag="PWU81_000326"
     CDS             complement(83298..84233)
                     /locus_tag="PWU81_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1109063.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(84549..86198)
                     /locus_tag="PWU81_000327"
     CDS             complement(84549..86198)
                     /locus_tag="PWU81_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL1109064.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(86315..88918)
                     /locus_tag="PWU81_000328"
     CDS             complement(86315..88918)
                     /locus_tag="PWU81_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL1109065.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(88930..89979)
                     /locus_tag="PWU81_000329"
     CDS             complement(88930..89979)
                     /locus_tag="PWU81_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDL1109066.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(90005..90319)
                     /locus_tag="PWU81_000330"
     CDS             complement(90005..90319)
                     /locus_tag="PWU81_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDL1109067.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(90335..90781)
                     /locus_tag="PWU81_000331"
     CDS             complement(90335..90781)
                     /locus_tag="PWU81_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1109068.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(90782..92704)
                     /locus_tag="PWU81_000332"
     CDS             complement(90782..92704)
                     /locus_tag="PWU81_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDL1109069.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT        26484 a        19599 c        16323 g        30419 t
ORIGIN      
        1 tcattcttac atgctgacag caaagtcagg ggcaaaccat tgaactgaac caacttttac
       61 gctttcgcct ggttgaggag catggatata ttgtcctccg cctaaagcaa tcgctacgtg
      121 gtaagttccg ccttgtgacc cccagaataa caagtctcca gcttttgctt gagaaactga
      181 aatttttgtt ccagctgatt cttgaggaac tgtccaacca ccgatgtcac gaccagttac
      241 ttgcatgtaa acatagcgtg tgaaccctga gcagtcaaag ccacttggat ctttaccgcc
      301 ccaaacatat ggagtaccaa tatatttgta agcttctgct acgattgctg caccattcac
      361 acttccagaa ggagctggag caggtgttac aggttttggt tgttcaggtg ttggtgttgg
      421 cgttggagcc ggagtactac ttcctgtgcc gtttcctgta tcaacacttt gatctgttga
      481 tggtgttgta ggcgcaggtg ttgttgattc ttctgttgtt gaagattcag gcactgttgt
      541 tgattcttct gttgctgaag attcaggcac tgttgttgat tcttctgttg ctgaagattc
      601 aggcgctgtt gttgattctt ctgttgctga agattcaggc gctgttgatt cttctgttga
      661 agattcaggt gttgttgatt cttctgttgc tgaagattgt gtagctgatg atgattcttc
      721 agtcattgaa gattgtgtag ctgatgattc ttctgttgct gagctttgtg cagtagatga
      781 agcttgagta gcttgtgctt cagcctctgc ttgttcgcgt gcttcttttt cagctttttc
      841 ttgtgcagct tgttgacgag cttgttctgc tagacgttgt tgttcacgaa tacgagcttg
      901 ctctgcttca gcttctgctt tttgacggtt caagtcagct ttcttgtctt cagcagttgc
      961 ttgttcagca gctaatgaag ttttcagtac gttcaaatca gcttgtttag ataaaagatc
     1021 gcctttttga gattccaaag cagcttggtt ttcagctaat tcttttagtt tagcatcgtt
     1081 ttcagctttt ttgtcttcta cagctttttt atcttgtttt tgttgttcca tcaagtcgtt
     1141 gtttgctttt accatcgttg tcattgcttg aacgcgtcca atagcatctg ctaatgaatc
     1201 agcattcaat acagcatcga tgtagtttga gcttgtgttg cttacttgtg cttcgcgtgc
     1261 ttgtttttgg attgtatctt cacgtttttc aatacgttct tgtaaatctg caatgtcttt
     1321 tactaattgt gcagattctt gacgcaatgt atcttgtttt gctaataggt cttgtgcttg
     1381 tgtgttgatt tcagaaactt ggctttctaa ggcatcgatt tgtgattgtg catctgcttg
     1441 ttgatttttc aagtctgcaa tcttttgatc ttgctgttgt atctgagaat caaaatcgtc
     1501 cgccgcggca gcgatcggtg atgctacggc agtcaaggtc attgaacata ccattactgc
     1561 tgatattaaa ctctttttca ctcttcattc ctccgactgg cttaatttaa taaatatcta
     1621 aatatattta agtctttctt aacgatataa aaattgtacc atagcagtgt gtcaacgata
     1681 taatagttat gttacaaaaa tgtttcaaaa caacttttaa aaatgacaaa actgttcgtt
     1741 tcagaaaatg atcatgaaaa agccgcgtta agtctagcat ttcttaagat aacaacgatt
     1801 ctttttcgct ttcgacctaa aaataaaaaa agaaattatt catcactgaa taatttctta
     1861 aagggaaaaa tgaataatac aaatataatc atgttcaaca gcagtgtagg tcctaaaaac
     1921 cttgtaataa aatacgtgtt atttactact gctaatttaa aaatcaactg gaccaccatc
     1981 gtaaacaatt cataacccgt gacaaagatg atcatactga aaaattcagt aaagatgttg
     2041 acatggataa cgtctttcat tacatacatc aaccatacaa ttaaagggag agctactgca
     2101 tagataccga taacgccaat atagtaagcg tcatagatag ccccgagaac aatcgtggta
     2161 atcagtaaat aacgtttttc aaacgccata ctgcaacata ataatgccaa tatcagaaag
     2221 tgggcatttg acatataggt gcctttcgac cactccccta gcattcttgt taaatgacca
     2281 tctatcaaca tcaaaaagaa caaaacgatt ggcagatagt acttcattgt ttctttcttc
     2341 aacatggact aatcctccgc tagccgttgg acaatcgtca caactgacac gtcatacatt
     2401 tctgcataag gtttcacata aacttcccga tccagcccat aactatctgg tttcgtttta
     2461 ataaccgtac cgatcggcaa gttagctgga gaattccctc caagaccgga tgtctggaca
     2521 acatcccctt ctttgatatc cgtatcacca gttaattggg tcaccactaa ggcatggagc
     2581 ttttcatcat agtttttaag caaaccaaac gcttcgccat tagccgaaga tacccgtact
     2641 ggaaaatgat tggagctttc attagaggat gacagtaatt cgattttaga cgaagccgta
     2701 ttgacctcga tcactcggcc aatcaatcct ttttgcgaca tgactgccat atttgcttcg
     2761 ataccgtctt tagaaccctt gtctacgatc agcatgtcct gccaactgtc aggcgaacga
     2821 gtaatgacat ttgccgttac tttttcgtaa ctagtcagcg tctgattcaa ctcaagttct
     2881 tttttcaacg cttcgatttc tctctcgtaa ttcttgtttt gctgtgaaac gtcatcatag
     2941 ctatcgattt tttcttttag ccgctcattt tcggcataag ttgaaaaaag attatggaca
     3001 gaatctactc catttcgtac ccaccgtact ggggcagaga ttgttttatc aaccattgca
     3061 acactgtcat taacgatgga ttggaagaaa ttgggctttc cttcattcgc tcgctgcgca
     3121 gcggtcacac ttagtatcgt caccacgatg ataacaataa ccaaggtaat aataatattt
     3181 ttattaggat tgaacttctt cacaaagaca cctcgaatta attagactct tgtgttcaat
     3241 tttatcacta aatgccgtga aacaaaaggt aagttgctcc tcttaagctt ttttttagca
     3301 aataatagat tctgtcattt ttagcctgaa agacaaattt ttttatttgt ttatggatca
     3361 atctttgctt tgaataactg caatcatacc atctgaaaaa gaaaattctg cggtatctga
     3421 aagaaattcg ttacttgcat aagatgtcgg atattctact tctttatttt ccaatacata
     3481 cttgctgcct tttaatgtaa gattcgagac gggagtcaag cagcaataag ctaaatactt
     3541 catatctggt atctgtctta ctatatgctc tccaggcaaa taataagaca aataattttg
     3601 acaatctctg ataataagct gatgggcaaa gggtttgaag cgaggttcca tacctaacca
     3661 gagattggct aaaagatgat ccaatcttcc gcccgtagct ccaatcaaag tgacttccgc
     3721 ttgcgggtaa cgttcaaatg tttttaagat acctaactgt gtatctgtat cgtctttttc
     3781 tgcaggggaa cgggtatact ctttggcttt tgcaaatact tccttctgct cttcgttcga
     3841 caatgaatca aaatcaccca ctgccagatc tactgggtag cctgcttgaa gcaggtacag
     3901 cgcaccacgg tctatcccga cataggcatc gtatttggac agcagagact gcggccagtt
     3961 ttgcggatta cctccagcag ctaataaaat tttcatttag tctaatgcct cttttaatcg
     4021 ttcgatttgt ccgactggat tttcggctcc atagatatat gatcctgcaa cgaagacatc
     4081 cgcccctgca tctttacaga tttttgctgt ttctggaacg attccgccat ctacttcgat
     4141 tgcataagtc caattgttct ctctacgtag atcagcgagt tccgtaattt tttctactgt
     4201 ttcttcaata aagctttgtc cgccgaaccc aggattgaca gtcatgacta gtacttgatc
     4261 agccaaaggc aaaacatgtt tgatcatgga gactggtgtt cctggattga taactactcc
     4321 agctttaact cctaagcttt ttgtcatttg aagtgcccga tgaatatgag gagttgcttc
     4381 gacgtggaca gtaatgatat ctgctccagc ttttgcaaat gctggaatgt agttttctgg
     4441 atcaacgatc atcagatgga cgtctaacgg taatttagta acaggacgga ttgcctgaac
     4501 aatatttggc cctaatgtga tatttgggac aaatcggcca tccattacat ctacgtggat
     4561 gtaatctgcg cctccttttt ccactaactc aatgtctctt tgtaaattgg caaaatctgc
     4621 actcaaaatg gatggtgcta tcttcattct tgtttcctcc tattttcgtt taccttcttt
     4681 ttttgcatac attggttttc ttttttcgat ttcttgtaag aattgcaaat aattgtcgta
     4741 acgtgtctgt gcgataccac ctgtttctac tctatgtttt acttcgcagc caggttcttt
     4801 agcatgcatg cactctctaa atttgcaatg gacagaagct tctacaaatt caggaaattg
     4861 tctcggcagt tctcctgctt caattgtcaa aaagtcaatc gagctaaatc ctggtgtatc
     4921 tgctactaac ccgccataga taggaatcag ttccacatga cgtgttgtat gccgtcctct
     4981 tccaagtgca tcagaaatct ctcctgttgc taaattcagt tccggtgata tttgattcag
     5041 tagcgtcgat tttcctgctc cagactgacc cataaagacc gatagccgtt ctgggaagta
     5101 acggatcagt tctgtcgtat cacccgtatg attgggtaca atcaccggat agcctatttt
     5161 agtataggtt tgcttgatct cttcgatttt ttctgggtcg tctgacaagt cggtctttgt
     5221 caaaaaaata atcggttcga tcgactcgta ttccaaggta actagaaagc ggtcaagtag
     5281 attataagaa aatgcaggct ctacgatact tgtgacaatg attccaagat caacatttgt
     5341 gactggtggg cgaactaatt gattttttct cggcaagatt tccagaagat agccgtctgt
     5401 ttgattttca ctctcaaaga tcacctgatc tccgacaagc ggcgtgattt tccgattccg
     5461 gaaatttcct cttcctcttg tttgataggt ctctccgttt gcgtatacat agtaaaaacc
     5521 gctgattgct tttctgattt gtcctttgat aagtgccacc tccgtttttg tttattttac
     5581 catacgaaga ggaaagtagg tgttattcca tgcaaacttc tcaaagtata tggcttgaaa
     5641 attgatcaaa agagatagaa acacaaaaaa gagaatgcaa caaatcaatg tcgcattccc
     5701 ttctgatata ttttattcag cacttatttt gaactttctg aggtgctagc tgatgaactt
     5761 gtggagctgg aagattcttc ttttgaagag ttgcttgtgc tactggagct acttgagctg
     5821 ctagaactgt ctgaagagtc tttttcagaa ctagaagacg ctggatctgg tcctttagag
     5881 taaattacgt tcacaaccat tcctttggta attgtcgttc ctgcccctgg tactgtacga
     5941 atcactttat ctttatccac cgtgtttgaa tattcggaag tctcatgccc gatatattct
     6001 gcaccgacac tagctaaata actttgtgct tcgctttttg tatagccgga gatatcattc
     6061 aatttaggct ctgttccttg gcttacgaca aaattgatgt aagtttcttc tgctacaact
     6121 tcacccgagg ctggatcctg actgatgatc gaacctgatg gtacagtgtc gctatagtca
     6181 taagtagcac taatattatt gatattgaat ccttgtgtaa caagccaatt acgaacagtt
     6241 tcgtagtttt ctcctacatg actactcaaa gtcactgttt ctggtccttt gctgactgtg
     6301 aaagtaatcg tgtctgtttt aggatcgact tctgtatctt ctgatggaga ttgcgaaatg
     6361 atcattccgc tttctacctc tgaactgaat tcctcttctt tttgaatcaa gtcagcagaa
     6421 aaccctaatt ctttcaaact tgcttcggca tcttcataac ttttaccact gtaatcatct
     6481 aatttgattt ttttcgtgcc gctactaata tacagagtga cttcgcgttt ttgcttgact
     6541 gtcgttcccg ctgccgggtc tgtcttcact acattacctt cttcgatttt gtcatctgca
     6601 atttcttctg tttttgttgc tacttctaat ttcgcttttg cgagtgtttc tctagcagaa
     6661 gcttcagaaa gatcagaaac atcaggaaca acaacttctc ctcgcccgcc agaagcaaag
     6721 aatacagctg ttccgattcc taaaacagca agaatagcta ataggatcag ccaccatttt
     6781 ttctgtcctt tctttttagg atcaggcaga acttcttcct cctctggtaa ggtttcgttt
     6841 tctggcttgt cttttggcag aggcatagat ttgaatgaat caggcattgg tgcatcttcc
     6901 gtaaggggtg tgataacttt tgtttcatta tccatcacat gaggctgcca tttttcttcg
     6961 tttgcacgag ctggtgataa tactgtagaa agatcatcag acatttcctc ggctgactta
     7021 taacggtctg caggttcttt agccgttgcc cgtaaaatca cgttttccag tgcttgtggc
     7081 acatttggat caagtgcttt tatagatgga agatcatctt gaaaatgttt taaagcaatt
     7141 gttacagcag attcaccatc aaacggtacg ctacccgtca acatctcata aaggatgatc
     7201 cctaaagcat aaatatctga ctgcttcgtt gccatacttc ctctggcctg ctcaggggac
     7261 agataatgaa cagatcctag catcgtattc gtctgcgtga tcgatgtttc agataaagcg
     7321 atagcaatcc cgaaatccgt gatttttaca acaccttcat tatcgatcag tacgttttgg
     7381 ggtttcaaat ctcggtggat gatttgatgg ttatgagcta aagaaatagc agaaaggatc
     7441 tgctgcataa tatttactgc tgtttcataa ggaacaggat aatgtgtttg gatatatcgt
     7501 ttcaagtcca ttcctttgac gtattccatc accagatact gcatgttgtc ttcttcacca
     7561 acatcgtaaa cactaacaat gtttggatga acgagttctg ttgctgctaa agcttctctc
     7621 tgaaatcgtc gaatcgcagt ctggtcattt tgaaagtcaa atcgtaagac tttaactgct
     7681 acatcacgat ccaagattaa atcgtgcgcc aaaaatacat ttgccatacc accgctaccg
     7741 atatttccag tgatttgata acggccattt aattttttgc ccaactcgat catacactgt
     7801 cctccttata ttcgataaga agaacggtga tattatccgc tccgcctgct tcattggcac
     7861 gatcaatcag ctcagttact ttatgagaca atgggccttc ttgattgatg atcgttccta
     7921 tttcttcttc agacagcatg ttcgtcaagc catctgaaca taacagcagt cgatctttta
     7981 gttgccaaag atgagtagaa acatctactt cgactgttcc aggcatgccg acagaacgtg
     8041 ttaaaatatt tttccttgga tggttcactg ccatttcttc gctgatttct cctgatttga
     8101 ctagttcatt gactaaagaa tgatcctccg tcaattgcat gattttatct tcacgaatca
     8161 aataagctct gctatcccca acattggcaa tcgtgaacca gtcatctgac agagcggccg
     8221 ctacgattgt tgttcccata ccgttatatt ccggctgttc ttgtccacgc tgataaatca
     8281 gcatattttc ttcttgaatc gattgaatga accattgggc gattttttca tcgtcaacag
     8341 cttcttgttt ttcccaagct tcgcctagat tcgttaccgc catctgactg gcaatgtctc
     8401 cggcacgatg accacccatc ccgtctgcta atacagcaag cttgagacct gaacggctgg
     8461 taaatacact ggcatagtct tgatttgtgt tacgtcgtct tcctacatct gattgatatt
     8521 caatctgcat tttttcacct cattactttt tgcgcaaaca acaaatgaaa aatccatctg
     8581 tgtaaaactg atgcggatac aaagtcaaca ttttctcttc gattgatgat tgtagcatag
     8641 gattggtcaa aacatcaatc ctctcaaaat cagggtgtct ttccaagaaa gcagaaacaa
     8701 cgtcttggtt ttcttctttt aagatcgtac aagtgctata agtcagaata ccggaagact
     8761 ttaaagttgg tgcgcagctt tctaaaattt ccaattggat ttttggtaaa ttcgctaatt
     8821 catccggctg ttttttataa cgaatatctg gttttcgccg aagcagccca agacctgaac
     8881 aaggcgcgtc tactaaaatc cgatcaaact gctctgcttc gaactgttta ctgacttctc
     8941 tagcatccat cttttgagcg tagacagcag aagagacacc tagacgtgct gcattatctt
     9001 caatgagttt gactttgtgt tcgtgtacat ctaaagcgac cacttctcca ccgcatgacg
     9061 gatctaaaaa agaagcaata tgcgtcgtct tacctccggg tgctgcacag gcatctaaga
     9121 catgatgttc aggctcgatt tgcatagacg gcgctacaag catcgaactt tcatcttgga
     9181 tcgtcatgac gccatcgtgg aacaatgagc tagaagctaa atgccctttt ttcgctacta
     9241 taccgtatgg actgacttgg cttttttcag ctgtgatctc ttcttcttgc aaaacagtga
     9301 tggcctgatc tctggaaagt cggcgtgtat ctactcgggc actagcatgg ctaggctgat
     9361 atagcgataa accgagcttt tcagtttctt cgtatccgat ttgatccacc agtcgtttcg
     9421 tcatccatag aggcaggctg attttgacag aaagtctttc gataggatca gcaatgcctt
     9481 gcagatctgg tgctccttga cgctgataat tcctcaagac accatttaca aatttccctg
     9541 tcccaggatt tccttttact tttgcgattt cgaccgcctc gtttaaaata gcatgtgcag
     9601 ggactttatc taaaaattct aattggtata cagaaagata aagaagcatc ttgacccagt
     9661 catccacttt ttttgcttta gcgatcaatg gtgcaacgta aaattccaat agtcgctgat
     9721 ggctaatcgt accatagatc aattctgtaa ataaacccac atctttttga tttagctcgc
     9781 tttgtttgat catctcattc aacaaaagat tcgagtaggc gccacccttg ttcactcgct
     9841 ctaatgtcag taatgccata tatcgtactg attttttcat ttttttaggg atttttttca
     9901 ttatttcacc tgctgaccaa tctgaacttg ctgaccgctt ccgttaagga aatcatgtac
     9961 ggcctgtttt cctttaccag ctggctgtaa ttcattgatg gctaagacgg tctgtttacc
    10021 gcaagcaatc catagatttt ttttgtcttt ccggatgatc gtaccaggtg ctttgtctgt
    10081 agtttctgcc aatacttcaa ccgataataa tttccagcgt atgccctcat aggtcgtgaa
    10141 tgcaattggc caaggacgca ttccgcgtac ttggttgtca atttcttctg cagttttatg
    10201 ccaatcgatt gcttcttgtt cccgtgtgat gtttggcgaa aaagttgctt ctgactcgtc
    10261 ttgaggtact ggtttttgtc catccaaaat attgggcaat gtttctagca atagctttct
    10321 tcctaaaagg ctaagctttt caaacatcgt ccctacatca tcttgtttag tgattggtat
    10381 gctttcttgc gcatagattc cgcctgcatc catttttttg atcatttcca tgatcgttac
    10441 accagtttct ttctccccat tcatgatggc ataatgaaca ggcgcacctc cccgatattt
    10501 aggaagtaaa gaagcatgga cattgattgc ccctaatttt ggtacttgca ataacttttc
    10561 aggcaagaat tggccaaatg ccgcagtgac gatcacatca ggcaccaatt caatgatttc
    10621 ttccatttct ggcgaaccgg agattttctc tggctgcagc acacggatac cgtgcttcag
    10681 agccgctttt tttaccggtg tcggcgtaat tactcgtttc cgcccaactg gacggtcggg
    10741 ctgtgttacc actgcctgga tctcgtaacc tgattctatc agcccttcaa gaatcggcac
    10801 agaaaaagca ggtgtgccca taaatactag cttagtcatc aagatgttcc tccatatatt
    10861 cttcaagttc ttcttccggg atctgctgta tcattttctc aatgaacaag ataccgtcta
    10921 agtgatcgat ttcatgctgg aaggcacggg ctaaatagcc aaaagccgtc acttctatct
    10981 cttctccgtc tcgatcataa taacgcacgg tcacttcatc tgctcgcttg accgttccat
    11041 agacatgcgg aatactgaga caaccttcta cgtcaaggct ttcacctttt gcttcgatga
    11101 tttctgggtt aatcagttca aatttctcgc cttcatctac ttcgatcaca gcaattctct
    11161 tgttttgtcc tacttgagga gcagcgatcc ctattccgtc attcgcaatc attgtctcat
    11221 ataaattgtc taataaagcg attgtctcgt ccgtgattac atcgattggc tgagcagttc
    11281 tttttaattt atcattaggg tggatcaata taggatagcg cataatttgg ccctttctta
    11341 aataaagttt aatggttcgt tgtcaattgc cacaaaaaga ccttgtcgct gttctttctg
    11401 gcttccattc aaaatttcat ccaaaacatg aggaagattc ggttcatgtt tatatttgat
    11461 gattagttga taataatatc ggtttttcac tctcaaaatc atgctgggcg ttggccctaa
    11521 tagcaagctt tgagggctta acacttcttt tagctgattg gcaatctgaa agattttctt
    11581 tgccgctact tgttcttcag gatggctggc ggttattttc acagtaaagt aataaggagg
    11641 atagcctcca cgatgccgta catgcatttc ctgctgataa aactgctcat aattttgttt
    11701 tttagccaat tctatggcgt aatgttgagg attgaatgtt tggatcacga cttcgcctgc
    11761 tttttccgct cgccctgctc gtcctgaaac ttgggtaagc aattggaatg tatgctcact
    11821 agaacgaaaa tccggtaaat tcagggaggt atcagcattc aacaccccaa ctaatgtcac
    11881 ttctggaaag tccaaacctt tagcaatcat ttgtgtccct aataatatat ctgcttcttt
    11941 tgcaccgaat ttttgtaaaa tctgttcgtg tgcccctttt cttcgagtgg tatctacatc
    12001 catacgcaaa atcctagctt gcggataaag ttctcggagt tcctcttcta ctttttgagt
    12061 acctgttcca tagtagcgga ttttattgcc gccacaatta ggacagcgat tgggaatagg
    12121 ttcttcatgt ccgcaataat ggcagcgcat tgaacgtgta tccatatgca gtgtaagaga
    12181 gatatcgcaa ttaggacatg gaagaacaaa tccacagtcc ctacacatca caaaagacga
    12241 atatcctcga cgattgagca gtaggacagt ttgttctttt ttgtctaatc gattttgtat
    12301 tttttcctgc aaattagcag aaaaagtaga ggtccgatga ttctgaaact cttcacgcat
    12361 atctacgatc tcaatagctg gaagctgtgc agctgctttg gctcggtggt ttaactgcaa
    12421 taactgataa acgccttttt gagctcgtgc tcttgattct aaagagggcg tcgcgcttcc
    12481 tagaactatt gggcaatgat gatatttact gcgccaaatt gctagatctc tcgcatgata
    12541 acgcggtgtc tcatcttgtt tgtaacttga ttcatgttct tcatcgataa tgatcagccc
    12601 gatatttttc agcggggcaa aaatagcaga tcgtgcgcct actactactt gcgcttcttc
    12661 ccgctcaatc ttgcgccatt catcatattt ttcaccttgg gaaagtccgc tatgtaagac
    12721 cgcaactgat tctccgaagc gtcctttgaa tcgctcgacc atttgcggag tcaatgcgat
    12781 ttcaggcact aacataatag ccgttttatt ttctgacaaa acatcagcga ttgcctgaag
    12841 atatacttct gttttcccgc tgccagtgat tccctccagc aaaaagacct gatctttttg
    12901 ctgctgacct gctgtgatga tttgttcgac agcatttttt tgttctgcat tcagttccaa
    12961 tgccgtcgtt tggtcaaata ctcggtcttt ataagggtcg cgataacgtt ctgattctat
    13021 aaactccagc cagccctttt cttttccttg attcaagaca gcagtactga ttcctttatt
    13081 tttgaaataa gcaattggtt cttcttggct cagttcagat aaacacaaaa gaagttcttt
    13141 ttgtttcttg gcattttgcc gtaattttgc ccattcttct tcgatttgct tttcagttaa
    13201 caaagaacgg accaaacgaa tcgtttttac tttatttcgt gtatggactt catatttgat
    13261 atctactaat tgctcttggc gccagcgaat caattgagaa aggctgcctt cttcttctgc
    13321 ttccttccag gaaatcactt ctcgctgatc aaacaatacc tgaacttctg gtgtgtctgc
    13381 caaaggatag atcaatttat catattctgc tttcatcaca ctcggaagca tcgtttgtag
    13441 acaagtgatt ttgaatgcgt aagtaatttt tttcatataa tcagctaatg aaagtaattc
    13501 ttcattgacc accggagcaa ggtctagcaa acgaataact gccttcaaag atggattagc
    13561 tgattcttct gatttttgga tagccataac aaatccttgg acatgacgat tgccattacc
    13621 aaaagggact tctactcgca tcccgacttg caaagctgtt tccatttcag agggaaccaa
    13681 ataagtgaat ggctgatcgg tctgcatagt aggtacgtcc acaatgactt cagctgttaa
    13741 tgtcatggtt cgtttcctcc tttttttgtc ttttcttatc aagcgacaaa aagttccttg
    13801 cctattttac tagagatcga tggatttgtc ttatcttctg ccaaatccta acaaaaaatt
    13861 aacagcagat aagaaaaaaa agaactgtgt caaaaagttc agacacagcc ttttctacca
    13921 gacaataagt ggcgaatgtt tgaaaaggaa acagctgggt ttgcttttct ttttagatgt
    13981 tcgctctatt tttctttatt acattttttg ctcttcacgg atacgtgatt ccaattcgtg
    14041 ttgttcacgc tctttttgtg cttgacgttc ctcttcttcc atcttcaaac gttcacgttt
    14101 taattcagga tgtggatcat tgactacatt tcctgcatcg atttcttcta aagcttgacc
    14161 gacgcttttt actgaatcaa aagaatctag agtaggctgt gcacttgcat ccaattcatg
    14221 cgcgcgttta cttgctaaaa tcactagaga atattttgaa tttacacgat ctaatagtga
    14281 gtcaattgaa ggttttaaca tcataagact acatctcctt taacatttta atatatttac
    14341 cgattacgcg atctactcga aaatgttcac tggcaataat gtttttgatt cgttcaactg
    14401 ctagcggcac ttcatcattt acaacagcat agtcgtaaag agccatcatt tcgatttctt
    14461 cttttgcaac tttcattcgt ttttcgatta cttcatctgc atctgttcct cggcctacga
    14521 tccgtgattt taattccgct aaatctggcg gagtcaaaaa gatgaataca ccatcgggca
    14581 ctttttcttt gacttgtttt gctccttgga cttcaatctc caagaaaaca tcttttcctt
    14641 catccaatgt cttgttgaca tatgacaaag gagttccata ataattcccg acatattctg
    14701 cgtattccag catctctcca gcttcgatca ttgattcaaa ctcttctttg cttcggaaga
    14761 aataatcaac gccttcgacc tccccttcac gcattttacg ggtagtcatt gagatcgaat
    14821 actgaaaatc attatcctca ctctcaaaaa ttgcttttcg tactgtgcct tttcctacac
    14881 cggatggacc agacaacacg attaataatc cacgctctga catgacgacg tcctttcaac
    14941 tagtcttttt gtacttatcg tttattttgc actttttttc tgcaactttc aaggttatct
    15001 ttaattatag ctagaaattg gtaaaaaagc taccctgtac caagaggtcg taaaaaagct
    15061 gtcctgcatc aagtaataag aacagccaaa cataaacagc ttctcatgtt tttcgtttgg
    15121 ttggggttat tatcgcagat gcaagctttt gaacgccgtg tataagaggt cgtgaagaag
    15181 ccgattagtt tcgagtatga agaaaaattt tgtgaaaata gtttctcaca tttttgcata
    15241 aatttggctt aataccgaga aaatggtttt tgaacatcac ttattcaaaa taaaaaaaga
    15301 ttgtgacacg aattttgtca caacctttaa atgtattcct agagcaaaga agctaagcga
    15361 agaaatccaa ttctgtttat ttattgtatt tctttgttca gcatccttgc taaaagccca
    15421 tattggacaa agtattcact acacccatga tctcatcttt tcgagaacgt gctttttctg
    15481 tcggatcttc tacctcttct gtgtcaattc gaacaataaa tttccgattc atcgtcacgt
    15541 aagcaccgtc gttgatcaaa ataaacagtt cgttctcatt atagtctgca caggcatctt
    15601 tccatacgtc aaagccgttt ttttcagaag ggatatcccg tttaaccaca gtttttccac
    15661 ttaattcgtg gaacgttacg tcataatatt tcattatctc tacctctttc gtcattcaat
    15721 attttgtact tgttctctga ttttttctaa gatcgtctta gactggacga cttgattttt
    15781 aatttcgatt gcatttgatt tggaaccgat cgtgttgatt tcccgattca tttcttggat
    15841 caggaaatct aattcccgac ctacaggaca ttgtgcttcg ataagttcat ctagtttttg
    15901 tacatggatc gccagacgat ccagttcctc gtgaatatct ccgcgttcca acaagatggc
    15961 tagctccgtc aacaaccgtt gctcatcgac tttcgctcca agaaattcct ccagcttttt
    16021 ttgatacctt atttgaaatt ccttttcata gatttctaca aaactatcga gtttttttag
    16081 tacttctttg aattcttcag tattcttttt caacactaaa gctaaagctt gaccttcttt
    16141 ttctcgactc gcctcaatag aatcaagtgc gcttttcaat gtatctagag ctaactgtgc
    16201 caattcttcc atgctctctt ccggtttttc ttctaccgtt acgaagtttt cgtccagcac
    16261 gaagcgttcc gccatccgac taattgacac ttgattttga ctaccgtatc gttgttgaat
    16321 tccgtctgtc aattgagtga taagtgaatc aatcaattcc cagttgatga cgatctgttt
    16381 ttcgtttttg ccaatataag ctagattcac aaatacttcg attcgaccgc ggcttaactg
    16441 ctgtttaata agctgacgaa aatcgttttc aacaaagttc aggattttag gacttcgtat
    16501 ttgaacatct agaaaacgct ggttgacact ttttatttca atatctaact gatacgcttt
    16561 tgtttcgcgg atggctttgc caaaaccagt catacttttc attcgacagg gtccttcctc
    16621 attacatatt ttctattgct ttttgcagtt gttcaaactc ttcgttcgtc aatgtcaggc
    16681 ctttccccat tttttcatgg tctggcgccc aatctcgaag atcgaatttt ggcgggcgac
    16741 cattccagct gactaggttc agttctttac gccacccctt gttgttttct gacaatatag
    16801 cgatttcttc tacgatttca tatgaaaatt cttgtgccat ggttgttcct ccttgagata
    16861 atataaatat aggatcagta atgtgtcaac tggttgctta agcaattcat gctgcaaacg
    16921 atccttgcgt tttccttgta gcagataatc aaaataaaca gttttcgtgc gctcattttg
    16981 tattttttgc tggtattttc gataccttcg ccattcttca gtacttaatt gaccaatttg
    17041 tgccaataaa tcatccggca tactttttat atcttgttgg cagatcattt gaatcatcat
    17101 gtctacttga tagatttcat tgggcagtaa aaaatgtttt tttgctaaaa gttctagcat
    17161 cgccaatccg catgtctctg gtacgaccct gttcttatat aaattacgca gtttttgcca
    17221 aaattttatg cttttgaaaa aagataattc taactgaatt tcttttgtta agtaagacaa
    17281 aaatcctcgc cagtatgttc ttgcaggttt aacagaatga aatgtatacc agttcccata
    17341 gtaatagcct aatcgttttt tcatttccag atagcctaat tgtaaattta attggttcct
    17401 ttggctatca aaaagcagaa agctgcctag tgtccacaat gtttgacatt tccaatggat
    17461 aaaaatgtct ggcatacgaa tttttttcgc tgggcctggc ccttgcacat ctacgacaaa
    17521 tgcctctgtt gctcctttgt taatcgcaat atcaataggt attttgttgc gatacccacc
    17581 gtctgcatat ttattttttc cgatctttcg ataagccata gcgggataaa atgaagcaga
    17641 ggcgagtatc caatctggta tttcttctgt ttttgtctgt tggatatcag tcactacttc
    17701 cgctagttct ggcagtttag tagacaccgt atataaacgt attttgggat tatgtttgag
    17761 taaggacaaa tccaaattgt ctttgaccat cttttccaat ggagcagaag aaaccccgcc
    17821 ttcaattagt gctgttttcg tcatttggcg tctttctcga tagaagcgtt cacgaaggtc
    17881 ttcaactgca gccatttctg gtaacgcaag cacttgtctt gtcgacagct ttttccataa
    17941 attcacagct tttttcatat ctccttgtaa aatcaatgcg ccgttcaacg cacctaccga
    18001 ggtacctgtg ataatctcaa agttgatatc atgttccttc agcgcctgcc acacaccaat
    18061 ctgataagca ccgtgcgctc ctcctctacc taaaacaagt gctgtatgat agaccagctt
    18121 tttatttaat tgtgtggttc cttgatagcc gtttttatga aaaatacgca accattcttc
    18181 tgatagagga gattgaaata aaaaacaaca gttttcttta ataaaaaaac gagcgcaagc
    18241 ttccatatac cgaaaaaatt ttttccattc aaaagtttca ccttcaatca acagattatt
    18301 aaaaataaca tctcctctac ttttagcaat agtaaaatag acggtatatt ttttcgatac
    18361 tgctttaaaa gcacattgct tttttgcaga acgccaaaaa gcgtctctac tttcttgatg
    18421 actttgaaaa aatggaaggg gttgttttgc ttgctggata ggcgaataca gcgacatttt
    18481 ctgtgaaaag taaacttttt ttatctccat cgcgctctcc tttttcttca agtataccag
    18541 tacgtcataa aaaggtcatg agtctcgtat tcgactttgt agattccata agaaaacaat
    18601 tcataaaacc tcctttaaat aaaaaaatac aaaaatcaca aaaattaatt gaaaatgaga
    18661 gtgttttcaa tgaaaacgtg attttgtaat ggaattttaa aataaaagaa acaagctttt
    18721 ttaatgcaaa tgttgtaaaa taaaagagat taagaggaaa taagaggagg aacatgatga
    18781 ctgagtcagt ttatgtacat attgatacca caagtaatgc tgttttaaca aaagggctga
    18841 ctgctactga ctttgcaaac agtatcgtgc attttcctca aaacttgctc ttgcttgatc
    18901 catctgcttc tgctggagaa tatgaatccc atacagggct gaaagtaatc cgtggtacag
    18961 aaaatattca gcgtttcttt tcctcttctc gtaacaggcg tggattcgat gaattgaagt
    19021 ggatcgactt tactgattta acgatgctga aagaattgac acctttagaa atttcagaat
    19081 tattatattt tggtcatatg aaaacacatc ttcattcgcc gtttttttat aaactacaga
    19141 ataattttgt atacttcgat cttaatgatc agctgaatcg gatttattat cgctatttag
    19201 atgaatttta taggattttt gctagtaaat taagttcgat cttgtcagat aagatcaatg
    19261 taaaaaaatc ttttttcaaa aaagaaacac ctgttgaaaa aatcagtgtg gaattattga
    19321 aagaaatgcg taatttgatg caggaaggga tcattttttc ttttgatcaa gttggtttga
    19381 tcgatggtaa atatcgtatc ccaatctatg tgattgaaga ttatatttgg aaattaaaag
    19441 gcacccggta tcgagaagag gaaatcatcg ctactttaat ctactctgtc accgatcgac
    19501 actggcaatt ggtagaagat gccgatcttt cttatctgcc attgaagtag atctatttct
    19561 tatcaaaaaa taaaaaggaa acaagaaact aggacatgat tctgtctcaa gtttcttgct
    19621 tcttttttat ttacagctcc tgtcgtttat ctggcacaaa aatcgaagga gtcgtttgtt
    19681 ggatccctaa taaaacgatg atcgttacaa tagctgtact caacccgctg gcaccattca
    19741 ttacgaaaga aaagagccaa ggattcatcc cccatagtgc atacgctccc cagaaaacca
    19801 ctcctgcaat aaaatgccaa aaatatctgg aaagtgcgcc aatcaatgca ccaaacacga
    19861 tgctttttct aatttgtttc gtattatcgt ctacatagcc tttttggaca tttgaagcca
    19921 tgagccctgc tgcgcctgca aaagtatagg caatcgggta ctcaatcaac acttgaacga
    19981 cacttaaata ataaacttgt cccaacggaa aatgtaggat gccccaaatg aaacctgcca
    20041 gcatccctgc ctttgtccct cttcttaacg caaaaagagt caatggaatc tgccctaatg
    20101 aaatagaaaa actgctgccg atctggatcg gaatcaacga caacaccatc gctaacgctg
    20161 caacaatcgt tccttctacc caaactctat tttttcccat aaaaaaactt cctcctttga
    20221 ataaaaaagt cacaaaggag gaagcagctt tcgaaaatag tcaaacagct gtttattcac
    20281 aattcctacg ctcgcataac cgatacaggt acgaagggtt tagaaataga ttcaatctca
    20341 gcccaattgg actccccttt gtgagtttat ttattattta ttttttgttt caaacaatgg
    20401 actaactggt ttgttttcat ggatacgttt gattgcatct cccattagtc cgccaacact
    20461 tacttcatcg attttgtcga ttttacgatc ttctggaaga tagatcgagt ctgtaacaac
    20521 tagacgctcg atagctgaat cttctattct ttgtaaagca gggccggata atactggatg
    20581 tgtacatgaa gcatacacac tagttgcacc agcttctttc aaagcattgg cagccaatgt
    20641 gatcgtacct gctgtatcga tcatatcatc gatcagtaca caagttttgc ctttgacttg
    20701 tccaatgata ttcattactt ctgcaacatt tgcttttgga cggcgtttgt ctatgatcgc
    20761 aatcggagat ttcaaaaatt ccgctaattt acgtgcccgt gtgacaccac catggtctgg
    20821 tgaaacaacg acaacatcat cgcctttgat tcctttttct aagaaataat tggcaataag
    20881 tggcgcaccc atcaaatgat cgactggaat atcaaagaaa ccttggattt ggactgcatg
    20941 aaggtccaaa gtcagcattc ttgttgcacc agctttttga atcatgtttg ctactaattt
    21001 cgctgtgatt ggttcccgtg cacgtgcttt acggtcttga cgcgcatagc cataataagg
    21061 catgactacg ttgattgttt ttgcacttgc tcgtttcagt gcgtcaatca tgatcagcaa
    21121 ctccatcaag ttatcattga ctgggctgct cgttgattga ataacataga catgcgatcc
    21181 acgaatactt tcttcaatgt ttacttgaat ttctccatca ctaaattgag taacagatga
    21241 ttttcctaac tcgacgccga ctgctgctgc aattttctct gctaatgggc gattggaatt
    21301 taacgcaaaa atttttaatc ttgggtcaaa gtaatgtttt gacatgggga cctccacttt
    21361 ctttttcaca actctagaat tctacctaat aaatagtagt catatttttt aaataaatca
    21421 agtgattttg caaaagtctt agtttagata agggagtttt tttgcatatc cttctttatt
    21481 gacttgtctt gctctcgcaa ttgccagcgc atattctgga atattgtctg taattgttga
    21541 cccggcagca atcgaactgt ttttagctac ctcgacaggt ccgatgatat tcgtgctaga
    21601 accaataaag ctgtggtcgc cgacagtcgt atgatgtttg ttctttccat catagttgac
    21661 gaagacaaca ccacatccga cattgatttc ttctcccagc gtagcatcac caacataagt
    21721 caagtgaccg actttagttc ctttgccgat ttgagcattt ttcacttcca caaagtttcc
    21781 gatatggacg ttttcgccaa tttcagcttt tggtcgtaaa tgtgcataag gtcccacatc
    21841 tgcatgtttc ttgacatggc tgctttcaat gactgagttt tcaatgacga cgtgatcctc
    21901 aatcctactg tctacgattt ttgaatgtga cccgatgaca caatcagaac caatgactgt
    21961 atttccttgg atttgtacac ctgcttcgat caatgtgtct ggaccgattt ctacgcctgc
    22021 atcgatgtat gtcgttgctg aatcgacaaa gcttacgcca ttgaccatat gcatctggtt
    22081 aattcgcttt ctcatgatct catttgcttt agctaacgcg atccgatcat tgactcccat
    22141 tgattcttca aaatcatccg tttgataagc agcaactgta tggccttctt cttttaagat
    22201 ttcgatgata tctgttagat agtattcacc ttgagcattg tttgttccaa ctttgcttaa
    22261 cgcatcgaac aaagcttcgt tgtcaaaaca gtaagttcct gtattgattt cttgtacaag
    22321 agcttcttct ggtgttgcat ctttttgttc cacgattttt tctacgatcc caatatggtc
    22381 acggatgatt cgtccatatc ctgttgggtt ttctgcttgt gccgtcaaga tcgttgcact
    22441 tgcatttttt ccttggtgat attcaaaaag attgttcagc gtttcagtag tcaataatgg
    22501 tgtatcgcca ctgataacta acgttgttcc tttttttcct tccaagaaag aagcagcttg
    22561 caaaacagca tgtcctgttc ccaattgttc tgcttgtaac gcatatttac tgcgttctcc
    22621 tagttgtgct ttaacttgtt ctgctccgtg tccaacgatc gtaatcactt catctggttt
    22681 cgtttcactg acccgattaa tgatatgttc taccataggt ttaccagaaa cgggatgtaa
    22741 aactttatat aattttgact tcatgcgggt acctttccct gccgccaaaa taattgcata
    22801 acgtgcgtcc aactgcgtca ctcctaaata tagtttgctg atttcagttt atcccagtat
    22861 acccattttt tcaacaaaac aagtgatttt cttttatccc agcgttttct ggataaaaaa
    22921 atagatagac cataacgggc ctatctattt ggtaaccggt gctctcctgc caactcctcg
    22981 gtcacaagtc acactactca taaaacatga aaagctgttt tttgaaatcg tttcttgtga
    23041 cttagagtta aacggtaggc ttacaacctc ttcattaaac gtttttcaat cagctgacct
    23101 tcggtattag ctcaataaac ggcaaaatat gaggagcaat ttttccgttt attgagctaa
    23161 tactcaggac aggacgctga tttcacaacc tctagataaa cggtgttcaa aagccagttt
    23221 ctcggtatta agccaaattt atgaaaaaat gtggaaagcc attttcacaa aatttttctt
    23281 actactcgaa actaagcggc tttttcacaa cctcttattc tgaaaaataa tttcctggca
    23341 caaccgtaat atttttcgtt tgagtatcta catctttgac ataaagcagt gacgtataat
    23401 catcgatggc gcgatgaccg ttgaattttg attcggcaaa tacagtgatg ccaacaagtt
    23461 ctgattcgaa ttcttcaatc aagctcttca ttccgttgac tgttccgccg cctttcatga
    23521 aatcatccac tacaaggaca cgtgaaccac gtttcaaact tctcttagac agttccatct
    23581 tttcaattct ttcagaagat ccagacacat agttgacact tactgttgat ccttctgtta
    23641 tttttgagtc ccggcgtaca ataacgaatg gcgcattcag ataataggac acagcttggg
    23701 caataggtac gccttttgtg gctacagtca ttacagcatc gattttttca ccaaggtatt
    23761 tagaagcaat gattcttcca acttgacgca gcagttcagg ttctcctaac aaatcagaga
    23821 gataaacata tcctcctgga agcagacggt cttgttctga tagacgttct gctagagatt
    23881 caatatattt tttcgcctct tcatatgaaa tttcagggat gaacaataca ccacctgcag
    23941 cacccgggat tgtttccaag gttccatacc ctcgttcttt aaatgttttc ttgatgattc
    24001 ccaaatcttc actgatagaa gatttagcag attcataacg ttcggcaaag cttgttaagg
    24061 aaatcaattc atgcgggtga ttcaataaat actgtgtcat gtcaactaga cgttcactgc
    24121 gacgtacttt cacctattcc aactcctttt aagaaatttt atcgttttac attatataag
    24181 tttttcttca aaaattcgag cattttatga aaaaataaga aaatagttcg gtatttatta
    24241 aaataaaaag aagcctataa aaaatttttc gtttttataa ggcttctttt ctttataatt
    24301 tagttatttt ttcttctgaa ggcaaccaat attcgtttga tgatatttac aatcaggaac
    24361 aaagcaataa agactaatgt gattgttgct cctggaggtg tatctagata aaacgagcta
    24421 gttagtcctg acagcatccc tagaaatcct acacaaacac tgatagcaat cacgacattg
    24481 aagcttttgc ctaatcgcat accgattgct gcgggaagaa ccatgattgc ggatatcaaa
    24541 agtgcaccgg caatcgggat catcacagca atagccacac cagtaatcac attgaacatc
    24601 atcgacatca aatgaacggg tagcccatcc acgtgggcgg tatcttcatc aaaagtcaga
    24661 acatacatcg gccgtttgaa taaaaagaac aatataccga tggcaacgaa taagattcct
    24721 aaaaagatga cttgctccca tgtaatggtc acgatcgaac caaacagata ggattggata
    24781 cttgttgctg aattccctcc gctcaaattc atcaatacta acgctagcgc caaaccacca
    24841 gccattaaaa tggcaatgga gatttctgaa taggttcggt agatcgttcg cagatactcc
    24901 aatatcacag ctgctaatac aacgactagc atggtcgtaa gtgtaggatt aaggttcaaa
    24961 aagaatccta acgctacccc tgctaaagag acatgagaaa gcgtatcagc catcagcgat
    25021 tgtcttctga tcactaaaaa cacacctagc atcggggcga ttccggcaat gaagatagcg
    25081 gcaagaaacg ctcttctcat gaattcatat gaaagcaacg ccattgagaa tcctcctttc
    25141 tcaccagtcg gatctgtctg tccgcatact gtttgatatc ttcatgatca tgggtgatca
    25201 ttagaactga tttcccatgg acatgcgcac tatgtcggag aagacgataa aattcatttc
    25261 tggattcttc atccatacct gttgttggtt catcaagaac aaataagtca gggtcagtag
    25321 caaaaatacg cgctaaactg attcgctgct tttgtccgcc agacaattca cctattcgcc
    25381 gatttcgcat atcccacatc cctacagctt ctaaagcacg ctgtacatgt tgatgatctt
    25441 tttcagagag acgcttgaac cagcggcctc ttggatatct tcctgaacgg actaattcga
    25501 tcaccgtact aggaaaacct gcattgaaag atgcaacttg ttgaggaata taaccgatac
    25561 ttaatttttt tccttcttga tttttctcag caatctttat cattcctttt gaaggtttca
    25621 gtaatcctaa cgatgctttc accaaggttg acttggctgc tccattttct ccagttaaaa
    25681 tgacaaattc acccgcatct acatgataag agacatcttc tagtacaggt tcatcatcat
    25741 agtaaaaggt caaatcagat atttcgatat aacgcacctt acaacctcca ttctaaaact
    25801 taattgatac tttcttttaa agctttcaga tttttttcca tgacagaaat ataattttcg
    25861 cctgctttga tttgttcatt agtcaagctt tctaaaggat tcaacacttc tagcttcact
    25921 cctgtttcat tcgacaatgt ctcagctacc ttcgaagagg cattttcttc aaaataaatc
    25981 actttgatgt tgtttttctt cacaaattct tttagctcag ctaagcgact tggtgtgggt
    26041 tcctgatctg gtgaaacgcc cgaaatcgct acttgtttta agtcgtattg attcgctaaa
    26101 taagcaaaag cagcatgctg agtgacaaat gtgcggtttg ttgcatcttt tagctcatcg
    26161 gagtattttt gatccagtgc ttcaagcttt ttgatataag catcggcatt cttcgtaaaa
    26221 gtttcctttt tatctggata tttttcgcct aattcttcag caatcgttct gacttctttt
    26281 atagctaata ccggatcgag ccaaacatgt ggatcgtatt catgtgaatg accatgccct
    26341 tcttctgatt cttcatgttc gtctgcttct tgcgattcta aacggtcgat tcctttagct
    26401 gcttcgatca tcaaggtctg tttgctatcc actgaatcct ttaacgaatc cacaaaaaat
    26461 tccatgtctg aactattata cacgaaaaca tctgcatcgg aaatttctgc tctttctttt
    26521 gcacttggtt cgaaatcatg cggctccgtt ccagcaggga tcaaaagttt cacgtttcct
    26581 tcatccccta cgatctcttt tgtaaaatca tacatcggat aaaaagtcgt aacgatcgtc
    26641 aaatcctctt ttttgtctgc ttccttgttc gtatttccac atgctgcaaa cagtaaagca
    26701 cctatcatta ccgtagctgt caaaagatat tttttcattt atatgctcct ttcaatgaac
    26761 aaatcggaat tttacgattt attaacgtga actaatgtat caaaataaaa aaacacagtc
    26821 aatagaaatc catcaatttc tgatttaagc atgtatattc ttgattaaat cgtaaaatgg
    26881 atgagttgct gctcatatct agccatttca ctacaataaa aaactgaaga ataccatttt
    26941 aggattcctc agtttttcat cacctatttt acttcttata ttttattgca gggttcgtac
    27001 taaataaact tcctcacaaa aaccttttaa cccattatat actctctgcg ctcttgaata
    27061 atgttgacac aaggcaaaaa cggttggtcc gcttccactc atcaatgcag catctgctcc
    27121 gtacttgatc atacgctctt tgatttgatg gatgactgga tgccgctttc ccgttatatc
    27181 ttctaaactg tttcccattt ttttgatcat ctgctgataa tcttgctctc ggatggcttc
    27241 agcaagtccg tggatgtctt gatgttggat ggcttgcgta tctacttctc taaacactgt
    27301 acgtgtagag acactcattc ttggtttgac caacacaacc cagcactgtg gcatagaagg
    27361 taatacttgg atttcttccc ctttgccacc gacaaatgcc gtacttccgt agacacagta
    27421 aggaacatct gtaccgactt ccaaaccgat ttctgccagt tcttctaacg aacagcccag
    27481 attccataat tggttgatcc ctcttagagc agctgcacag tctgtacttc ctccgcctaa
    27541 tccagctgct actggaatat tcttcttaac aaagatcttg acaccggttt tgatattgta
    27601 tttatttttt agcaataaag cagcttgata aacattgttc cgttcatcta ccggtaaaaa
    27661 tgctttattt gtttctacaa tgattttgtc ttctgttatt tcttcaaaaa atagatgatc
    27721 tgccaagtct acgctcgaca tcaccatttc caactcatga tacccatctt gccgtctata
    27781 taatacatcc agtcctaaat ttatcttggc tggcgctttt tcaatcaatt ccatcctctc
    27841 acccattcga aaagtctaag tcgcaagtat tctatcatag ggaaaaataa aagtatagct
    27901 tatgtctgcc tagtggttga ttttgtcatc gtttcaacag ctttcacaga attttctctt
    27961 cataatccaa acaagttcta gtgattctta taattctgtg agcacttctt ctgccaaact
    28021 cggaacattt cctttttctt ttcctatcag actgagtagc tgcatggtag caaatccgaa
    28081 attacttcct ttcctataag caaaataacg tccttcccaa tgatcgacat attttaattt
    28141 cttttcataa gcagactgga aggaatagaa aaaattacca taattatata cgatatgcat
    28201 gactttttct tgaaagaaag aaaaatctgt ttctccaaca ttagctaatg gagctaagcc
    28261 tagattggct tccgagtagc ccattttctc gtatacaata aacagattgg ctaacagata
    28321 gtcagctaat tcctcagatt ctttaggtaa gacccgcaac aaatcatatc ccgcctgctt
    28381 cttcgtcatc ggctgagcgg tgatgaagcc aacgactttt tgatctttac ggacaatacc
    28441 gacaccgcta gataatacat atgatttatc aaatcgtccc acagagaagt atttttctcg
    28501 tgcaccttga agccattttt gtgaaacttc atttacagct tgatgtaatt cttccggcag
    28561 ttgatcgtac caagtaaaat ggtatccttc tttcatcaaa cgttgatatt caagttgctg
    28621 actcagccga gacaagtctt ctgttgctag atcaacaatc cctgcttcac ctactttcgt
    28681 gaagtgaaaa cccaattcat gaagcatcac tacgtagctt tctgatatct tataaaacgc
    28741 aagctgatag cctaaacaat ctgcttgttt cataaactga ttcgttgctg cccgcatcat
    28801 tttaggattg ccaactggat ctcctaaaac aaagcatttg tttcctttga tctgataacc
    28861 aaaaacgact tgatcttggt cgtcttcttg gtaataaaaa taactgtaac ctgacaagcg
    28921 aagctgatgg cttgtagctg tgcctccgta tgtttccaat aaacggtcaa aacggtcttt
    28981 ttcgtactca ctgccaagct tcgtagtatc ttcagctaaa tattgataaa gcgccactaa
    29041 agctagcaat gagatgccca aaccaataag ccctgagaac cacacatcat ctgaaggaaa
    29101 caaaataaaa tgatttcctg ttattcgacc gactaaaaag cgtccgctgt gatagcctgc
    29161 aattgcgtaa acgataaaca aaaagccgaa caagctagca tcaaagatta tagctcccca
    29221 agaataaacg aattttgttc gataaaattc ttttctggat aaccacacag ctaacagaat
    29281 caagacatat accaatatca actgccaaga taacgtacgt gtgattgtat ttaaaatacc
    29341 aaaagatagt aaagcaatcg taggcagaaa tgcctttttg acttttactg aaagtgctct
    29401 agcaaggccg agtaataaaa agccaaccaa tagattcact gtctgatcta aaaaattgaa
    29461 tgagaacggc aataatactt gaaatagccg actgattgca gacagattag tgattgttga
    29521 tagaagtacc atcatgatcc ccgcaaaata gacagcactt acgagcacga tatgcgcaac
    29581 cttttggaaa aacagtcgcg gaatgttatc taaaaatcga ttgattttga ctcctgtatg
    29641 tgtgatgaac aatagaagtc ccgtcataaa aggtagaaca taataaaaca accgataata
    29701 taatagccaa actactgcta catcttgccg aacagacaat tgactcattc ctaagatcat
    29761 caaaacgtca aaagtcccca ttccacctgg gaccatcgtc agcattccta tcaatgtagc
    29821 aatcatgaac ataggataga tcgaaagcat cgaaatatct attctcatca acttgccgat
    29881 gatcaaaaat accgcaaggg ctcccgtcca ttgtccaaag gatgccagta gcagaccaag
    29941 caagcctttt ggataaaact cgaagaacaa tgtctttctc tttagataag caaaaaagaa
    30001 aatgactggt gctaaaaaac taccgccgaa aagccagatc caatagcttc gaaaaatact
    30061 ttctgactga ataaaaaaga catcaataaa tgtaagaaac gaccatacag acaaaccagt
    30121 cagcataaat agtgcgactt ttgatacagt ggctaaaacc atttttctat ttgtctgctt
    30181 tccataaaaa ctagcccgca gacttgctcc taccacacca ccaaaaccgg cgagattgtt
    30241 gatcgtgttt gttacccacg cagcaagaag aaactctttt ctttccattt ttggttttcc
    30301 ttgtttttct aagactctga tcgtcaccca atcatacaac aacatcggag tgacacccag
    30361 cagtccagcc agaatcatga gcaggatcgt catgcggctt tgttgtccca tcctttgcca
    30421 aatatcttgc caactcatgc cattagcaat atttgcgact tgatttgcca caaagactaa
    30481 cacggagccg aaaaaaatca gttttaaaat cagactatgt tcctttatcc attgcagcat
    30541 caagtatcgt ttatttttca cattttccca ttcctaactt gtatactttt gtgtcatacc
    30601 ccaatgttat cttatttatt ttaatttttc cactttagac ctgttctttg cttgcgatga
    30661 tagaaaaaga aagatatcct aatgctaaag gagaggataa catgaaagta ggatttatcg
    30721 gcacagggaa tatggcacaa gctattattg atggtttatt aaaaaaagga acagtctcga
    30781 aagaagaggt gttcgtttcc agtggacatt ttgaaaatgc acaaaaattt gctgctgaaa
    30841 ctggcgtcca tgcatgcaaa agtaatcagg aagttgcatc acaatcagat atcgttcttt
    30901 tagccgtgaa accaaaaatc attgaaaaag tattggaaga actaagagaa tatagccaga
    30961 aagtcttatt catttctatt gcttcaggaa aatcattaat agatttagaa acagcgcttg
    31021 aagatcctca ggcctccatc gtacgtgtga tgccgaatgt caatgtcagc gtaggagcag
    31081 gcgtttctgc gatttgcaaa aatgaagctg ccacggagga acagcttcag caagctacga
    31141 tgatttttga agcaatcggc agcgtctatc ctttagcaga atcagatttc agtacattca
    31201 ttggccttgc tggcagctca cccgcattcg tttacatgct gatcgatgcg atgggacgga
    31261 ctggtgtact gaatggtttg tccaaacaga cagccactga gattgctgca aaagcaatta
    31321 tgggttcttg tcaaaaattc ctttcaagtg ataaaaatcc atgggaactt gtagaccaag
    31381 tttcttctcc tggcggtaca actgttgcag gtgtagttgc cttagaagaa gcaggattca
    31441 tcccagcaat catcaaagga atcaatacga cgatcgagaa agatcttgag atgttgaata
    31501 aaaaataatg atagcaatgt tttttcgtgc cttacaaaaa aagaagagct gtggcttttg
    31561 ccgcggctct tcttttgtat tttttattat ttagcataat cagttgcacg agtttcccga
    31621 ataacgatta ctttgatgtg tccaggataa tccagttctt cttcgattcg tttacgaata
    31681 tcacgtacta aacgaacagc gtctaaatcc gagatttctt ctggcttgac cattacccga
    31741 acttcgcgtc cagcttgaac tgcgtaactt gattctacac cggcgaagct gttcgaaata
    31801 ttttccagac ttcttaaacg acggatgtag ttttctaatg attcacttcg cgcaccaggt
    31861 cttgctgctg agagcgcatc tgctgcagct acaaggacag agataacaga agtagcttcc
    31921 acatctccat gatgagaagc gatcgcgttg attactactg ggttttcttt gtacttggca
    31981 gctaattccg caccgatctc aacgtgagat ccttcaatct catggtcaag tgctttccca
    32041 atatcatgga gcaacccggc acgttttgct aattggatat cttcgccaag ctctgctgct
    32101 aagataccag ctaattttgc aacctcgacc gaatgcttca agacgttttg tccataacta
    32161 gttctgaatc gcagacgtcc gatgatcttg attagatctg gatgaagtgt atgagcaccg
    32221 acttcaaacg ctgcctcttc cccgtattcg cgaatacgtt catccatttc tttacgagat
    32281 ttctcgacca tttcttcgat acgcgctgga tggattcgtc catcttgtat cagtttttcc
    32341 aaggtcatcc gtgcgatttc tcttcggatt ggatcgaatc cggaaagaac aactgcttcc
    32401 ggtgtatcat cgatgattaa atcgatacca gtcagcgtct caagcgttcg aatattacga
    32461 ccttctcgtc caatgatacg acctttcatt tcatcattcg gaagggtaac gactgaaaca
    32521 gtcgtttctg aaacttgatc tgctgcgcag cgttgaatcg ctaatgataa taagtttttc
    32581 gctttgcgat ctgcttcttc tttggcacgc tgttcagatt cttttaccat tagagttaac
    32641 tcatgattca gctcttcttc agtcgatttc atgatgacat ctcgtgcgtc ctcttttgat
    32701 aaaccagcaa ttttttctag ttctttttgt tgatcttcaa tcattttttc aacgtctttt
    32761 tctcgctctt caattaattg ctgttttgaa ctaagtgctt cttctttact ttcaagtgag
    32821 ttctcgcgtt tttctaacga gtcatcttta cgattcagcg tttgttcgcg ttgaatcaaa
    32881 cgattctctt gagattttaa ttctaactta ctttctctca gctcactttc aacttcagaa
    32941 cggtatttct gattttcttc cttagcttct aacaatgctt ctttttttag agtttctgct
    33001 tctttttttg cactatcgat gattccagca gctgatgatt gtgcaccagc tatttctttc
    33061 tcgtgacgtg actttgcgac cataaaccct aaaccaagac cgacaattaa accgatgata
    33121 gcgaggagaa tgttgaatac cataaaatcc acctccatac tatctttttc atttttatca
    33181 tctatacagc ttctatgata aattattcta aactcgtcaa tatgcatctt cgcatatatg
    33241 tgtacaaaaa acatgcacaa ctttattcta atgtttgtac cccgtagtgt caactttaaa
    33301 taaacaaaga aacggctttc tacttattca ttaaacgatg gtttttaaaa taaatgacac
    33361 tagtttcata taggtaaacc tttaaattga gaaacttttc tattaaatta ttctttgtag
    33421 atgaacagat atttttattt tatgtaccca ctcgtataat taaataactc acgatttctt
    33481 tattcaataa aaaaacggca ggtcaaggag agtacctact cttcagacca gccgtttttg
    33541 tttatcataa tttttattct tctactaaag gcaattcttc ttgtcctttt tcatcttctg
    33601 ggacagcgac ttcttcacca ataccataag ctgctctaac tagagcagaa acttctgcca
    33661 tcatttcagg atgattagcc atatagattt tagcgttttc ccgtccttga ccaatccgat
    33721 cttctttata agaataccaa gcaccgcttt tatcaacaat gtctttttct actgccatgt
    33781 ctagaagttc tccttcttgt gagatcccta aaccatacat gacatctact tcagcgattt
    33841 tgaaaggagg tgctactttg ttttttacga ctttgatctt tgtacggtta ccaacaatat
    33901 ctgtaccttg tttcaattgt tctgcacgac gtacttccaa acggatcgtt gcgtagaatt
    33961 tcaatgcgcg tcctccagga gtgatttctg gattcccaaa catcacgcct actttttcac
    34021 ggatttggtt gatgaaaata gcgattgttt ttgttttgtt gatcgaacca gagagtttac
    34081 gcaatgcttg agacatcaaa cgtgcttgta acccgacatg tgagtcaccc atttcgccgt
    34141 cgatttctgc tcgtggaact aaagcagcaa ctgaatcgac tactacgata tctacagccc
    34201 cacttgatac taaagcatca gcgatctcta gaccttgttc tcctgtgtca ggctgtgaaa
    34261 gaagtagttc atcgatattc acacctaatt tttgtgcata ttgcggatct aacgcatgct
    34321 cagcatcaat gaaagcggcc gttccgccat ttttttgtac ttctgcaata gcgtgtagtg
    34381 caactgttgt tttacctgaa ctttcaggtc catatacttc gatgatacgt ccacgggggt
    34441 aaccgccgac acctaaagcg acatctaacg caagagagcc acttggaatt gtagagattt
    34501 gttggtcgat tttttcgccc aatttcataa tagagccttt accatagctc ttttcaattt
    34561 ttttcagtgc agcatctaag gctgctttac gatcatctgc caatacgata atcctcctta
    34621 tatttacaaa tcagagcaac atttcattat ccttatcata tcttttttag tgaaaaaaag
    34681 caagaaaaaa ccgaacaaat attcgcattt atcattttct taaaagctgt tttctcaaaa
    34741 gatcaaatcc ttgcataacc gcattatatc tgatagcagc acgatctctg gcaaagtgat
    34801 atttttccga aaccacgccg tctttagatg aaatagcgat ccatactgtt cctgcagatt
    34861 gaccttctaa cgtgtccggt ccagccactc cggtgaaagc tacactgtaa tctgttcttg
    34921 ctatctcacg tgcttgaatg gccatctgct ccacacattc tttgctgact gttccatatt
    34981 ctgctaatag ctccggatca atctccagaa aaccagcttt tgtttggagc gagtaggtaa
    35041 cgaatccgcc tggaaatact tcagatacac cagcaatgtc gcctaatgcg gcttggaagg
    35101 cgccggctgt caaactttcc gctgctgtta cagtctgctt gttttctttc agtagttcca
    35161 ctacgacttt tgctagcgaa ttatcatcgc cgtatccata aaaatattcc cctactcgtt
    35221 cttggatttt ttcttctaat gctaaaagag cttgattccc tgcctgaaca tcatttgttt
    35281 tcactgtcag tcgtaatgtc acttcatttg gtttggcata tggtgcgatc gttggattga
    35341 tctgtgtctc aatcagatct tttagctcag tgaccagtcg cgattcacca atcccataaa
    35401 agcgcaaaac tttagaaatt agtttttctt cagaaggaaa acgttcctct agtaaaggac
    35461 gtacttgttc cacaaacatt ggtttcaact cattaggcgg accgggaaga aggatatacg
    35521 catgtgtatc tgtttgataa aaagtcccta aagcaagccc tgtacgattt ggtagaggaa
    35581 ccccgccttc gataatctgg gactgttgca gattgttttt ggtcattttt cgatgggttg
    35641 tttcaaaata tgccaataat tttttgtatc cttccgtatt ttgtatgaga gacttaccga
    35701 ggtgagccgc agttgcttct tttgtcaggt catcttctgt cggtcctaat ccgccacata
    35761 agagtatgag ttcacttctt gtttcagcta gagcaatcaa ctcttctaaa cgctgctgat
    35821 tatctcctac aactgtttga taataaactt cgatacctag atcagctagc tgttctgaca
    35881 agaaagtggc attcgtattc acgacttgtc ctagtaaaag ttctgttccg acagctatta
    35941 tttctgcttt cattacttcc cgctccctta tatagtatac gctgtatttt tatatttttt
    36001 tatttaaaaa gagggcaagg agctgtgaca aaaatcttgt cacagctcct tgttccaaat
    36061 aaacagtgtt ctcctgccaa aacctcggtc ataagtcaca atatacataa aacatgaaaa
    36121 gctgtttttt gaaattatcc cttgtgactc agagttagac ggcaggctca caacctctcc
    36181 actaaacgat acttacattg atcctttgaa cacaccgctg tttttgatga agtaatccac
    36241 acctgaatag atagtaaaga tcagacaagc ataaagcatg atttgatcta ctggaatacc
    36301 gatcaggctg aatgggatat tattgataaa cagcaagata atagcaacca tttgtgtagc
    36361 cgtcttgact tttcccggcc aagctgcagc catgacttct ccatgttcga caagtagtaa
    36421 acgcaagcct gtgacggcta attcacgaca gacaatgatc gctactaccc aaccaggggc
    36481 ctttccttgg tcaaccaaca tgataaaagc agtcataact agcattttat ctgccaacgg
    36541 gtccgcaaac ttgccaaaat tagtgacaag gcctcgcgca cgggcaattt ttccatctaa
    36601 ccagtctgtg atactagcta aagcaaaaat aactgcacct actagctgtg tgacagccag
    36661 tgtggtgtct cctacgttaa attgtcccca atctaaaggt aaacctacaa ccaaaatgaa
    36721 aatcgggatc atacaaattc taagtacagt taatttattt ggtaaattca cactatcgac
    36781 tcctttcttc cctattctac gtgactatca gcgaagtttc caccgatatt atttactttt
    36841 ccaaatgaat ttattcaaag gagtattttt attgaaattg atctttttga attaacgaag
    36901 aaggaccgcg acacagattt gtcacgatcc ttctttttta tacctacaag taaacagatg
    36961 cgtaaactaa tttatacgat cattctgcgt attgaagagt aaggttcaca ttttttggac
    37021 tctgattgtt ttcccctgga ttaactggta cggattctcc attcgctttg atcgttgcat
    37081 tgcttgctgc accaatcgtg atggtcgcat ttgttgctgt ttccggtaac acggtactct
    37141 gtgtttcatt tgctgccagt gtcccttgat aaacataggc attgtctact agcacaccta
    37201 accatacacg attgacagca gtaaattcaa gggttacagg cttggtagcg tctgtgatat
    37261 ttactgcgat ggcactgcca gaatcttctc ctgtagctat tgccattttc ttttcttcac
    37321 ttgattttgt tgttgattct actgtagaag actctttggt ctgcgtgctt tgctctgagg
    37381 aagaggactc ctcgactgat ccttctactt taagcgaaga agctgtgtct ccgattattg
    37441 gatcagcttg acgatcttgc caagtcatat agcccacgac aacgacaatc gccaaagcaa
    37501 ccaatcctaa taaaatgaca ggaagacttg tccaaaagcg acttggatgc gattcttcct
    37561 gatgcattgc ttttctagaa gtacttactg tttcaggctg cggacgttca gggaccgatc
    37621 ctgtcaattt gcttttccca tcaaaaacat cgactaaatg gtctccatct tctcctacag
    37681 cttcagcgta ttgccgaata aatgcccgca cgtaaaattt accaggcaat tggtcaaaat
    37741 catcattgtc gattgcttcc aaataacgtt tttgaatttt tgtaatctgc tgtaattcat
    37801 ctaaagatat ttttttattc aggcgtgctt gccgtaattt ttctccgatg gtttcaaaag
    37861 ccactcgttc atcttctcca gtctgtttga ttctccgaag catctgctgt tgcaacgaaa
    37921 gacgttatgt ttgggtgctg tgacaaaagt catgtcacag ctcctttttt ccgaataaac
    37981 attgttctcc tgccaactcc tcggtcacaa gtcacaatat ccaataaata taagaagctg
    38041 ttttttgaac ttgttccttg tgactcggag ttaaacggca ggctcacgac ctctttctta
    38101 aacggtgttc aatcagctgt tcctcggtat tagctcaaaa accggcaaaa tataaggaga
    38161 tatttttccg tatgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttcct
    38221 taaacggtgt tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacattaa
    38281 gaagctgttt ttacttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc
    38341 ttggtacacg gtgttccaga agtagcttct tcggcaataa gccgaaatat cacaaaaatt
    38401 tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac
    38461 ctctttgatt acttcgtttg attttatttt aatataagtt gattaaaaga gagcatgatt
    38521 tgttttattt tctcactaat ttttccaaca caactaagaa taccataaaa gctgattttg
    38581 gaggaggttc ctactacaag tctaaaacga aatttaagac ttttcctcat aaaatactgg
    38641 tttattttgg tctcatgtaa aactcgctga aggcctcttc acttataaat gcttccccag
    38701 ctgccagtac gtctttcatt tggatcgaat cgatgatttc aggaagatca aataaagttc
    38761 gatcgccaaa taaactttgc gtaaattgat tggcaatata ttcgatcgaa tttaatgatt
    38821 ggaaatattg tccaagcatt ttcttcttca gcaaatcgag atttgtttct gacacttccg
    38881 gatcatcagc gaatccaaga atgatttctt tgactttttc tgctgctttc tctggttcgt
    38941 ccgtatctcc gctgaagtcc gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa
    39001 agctgtcatc gataattcct tgattgtaca tcgctagata gtttcgagaa gtattgccta
    39061 aaatcatttg gaacaacaga ttgattgctg ttttatagcg taacagttct gttccttctt
    39121 gcggcaatgt gtctaatcct ttaataccaa gtacaaattt atctcgtgta atggctgctt
    39181 ccaatgcact ttgtttgatg atttcttttg tgttttcagg gaaataacgt acaatttctt
    39241 gctttggagg gaaatttttt gcttcttggt tttcccgaat aagtttcatc aatttctcag
    39301 gctccatctt accaactaca aataaaacca tattgcttgg ctgataaaac gtccgatagc
    39361 acgtatacaa gtcttgagct gtgattttgt cgatactttc aaccgtgccc gcaatatcga
    39421 tatgcaatgg gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc cgccagttag
    39481 ggtcgtcttc atacatttgg atttcctgac cgatgattcc tttttccttg tttacagttt
    39541 cttctgtgaa gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc ttttctacct
    39601 gatccgtcgt agaaaacaga tagctggttt tcgtaaaact tgtgaaagca ttggcggaag
    39661 ctccttgttt cccaaataat tgaaagacat caccatcttc tttttcaaac aacttatgtt
    39721 ctaaaaaatg ggcaattcca tctggaactt tcaccttttc tttttcacca tagggaataa
    39781 attcgttatc gatcgaacca taattcgtac tgaacaaacc atatgtcttg tgatagtcat
    39841 ttttaggcaa caaatacacg gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat
    39901 tgatttgctc atattctgtt ttattcattt gcatttcctc ctgctaaaaa gaagattgcc
    39961 tgcaattcga tttgctcagc tacttgttgt atctctttga tcgttacgct ctccatacgt
    40021 ttcaaccatt cttcatcaga taattttgtt tccggcagcc agctgtccaa aaaagaagct
    40081 tcgattgccg cttgcggaga gtctagagaa agcagatatt ggtttctcag cattgctttt
    40141 gtctgagaca gttccaactc tgttatttca ccatttcgca aactttctaa ttgctcgtga
    40201 atcagatgaa gtacttgatt tcggttcttt tcatcgatac cggtttggac actcatgaat
    40261 cctcggaacg tatccacgct actagaggca taataagcta gactttcttt ctctctgaca
    40321 ttcatgaaaa gtttagagtg aggaaaaccg ccaaataaac cgttaaagac cattagcgca
    40381 aaacgttctg gttcatcgta atacacattt gtctgataag ctaggttcag ctttgcttga
    40441 acgattggct cacgtacttg tcgttccttc acgatattca cttgaggttg ggtataaaac
    40501 agatctggat aaactgtttc ccgcgactca aatggtaatt gcccaactgc ttcagctgct
    40561 ttgtccggat caacatcgcc gacaacaaaa atgtcgattt gatcatttgc catcatttct
    40621 tgatagtagg acgctagcga actagcagtg atcttgtcca aatcttcgac tacgcccaac
    40681 cctgggatct tttgttccgg gctgttttca aaataaagtt cttgcaaggc ataggaagca
    40741 aaagtttgtt tatcttcctt caaactttcc aaatatagac gcagattgtt cttttccaag
    40801 tcaaacgtca actgatcaaa ctgatgattt ttaatatttg gataaaataa aacctctttt
    40861 aaaaaatcta ccgcttgaga aaaaaggttt ggatcattga catattcacc attcacaaaa
    40921 ctgatccctg cgtttagcca atgtaaattt cctttttttc taactgataa tccaaagctt
    40981 gcgccataaa gctctgcaag ttgcgcactt aactttgtct gatccgaata attcaaacta
    41041 tttgtttcca acatgctgct gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc
    41101 gcagtaaaac ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga
    41161 ttcacgcctt ctgccaattg atacaccata tttatatgct ccttttcaca gttgattgcc
    41221 cgcttactct tttaatgggt caacctgcct tttttactta ttcctctgct tgaaatttat
    41281 aagttcaggc aattcgccgt tttttctaaa tgatgttaca ctttatagta tagcatagat
    41341 gttttttatc aaaagaaaca gacggctaaa agcgtatatt gtttttttat tttttatttt
    41401 tcgatatact aaagacaaat aaaagaaggg agggtcattc atgatcaaag aattcaaaga
    41461 gttcatcatg cgtggagatg tcttggatct tgctgtcggc gttgttattg gtagtgcgtt
    41521 tacaagcatc gtaaaccaag tggtgcaagg cttgatcaca ccactaatag gttggtttgt
    41581 cgcaatgatc acaggtactt ctgatttaga aggagcactt tcaattcttg actggtcgcc
    41641 gacaaaaggc gtcacatttg cgtttggtaa cgttattagt gctatcatta catttctgat
    41701 cactggtttt gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc
    41761 caaagaagag caagtggaag tggaagaaac tcctactgca gaagattatc ttagtgacat
    41821 acgcgatctt cttgccaaac aagtggcaga agacaataca catccagtag agcaaacaaa
    41881 tctttccgat acttctaaat aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc
    41941 ggctttcttt ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga
    42001 atatcatctt ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct
    42061 tgtaggtatt cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg
    42121 ttaatcgttt cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca
    42181 aggacacgtc caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc
    42241 ttcccatcag tatcttgaaa aaacgtatgc caagcttctc catcaatgtc atcaacttct
    42301 tgataaggac actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa
    42361 aactctgctg ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc
    42421 cctgcttttt tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa
    42481 tctcaggctc gtttgctttt taaactgctt cagttaaaaa ttcgatctca actgtccgag
    42541 taagtacatc tgaatagctg taagatactc gttcaaaaga attttcttca ggatccaaat
    42601 caacaacgaa tactgaagga taggtttctg ttaaaacgcc gcggcgttct gtttgacgct
    42661 ttcttcctgt ctgagcaacc aacatgattg ggctgccgat acgaccttct aaatctttct
    42721 tgattgaagc taaagttgtt ggcatacagt tcacctctac tatcaactat accacatatt
    42781 tttaaaattt tcaagtttac cataaaaaaa aaagaaatgc aataaaaata aataagaagc
    42841 tcattaagat ttttctaaat tattcctcat tataaaattt caagtgtttt tttattttga
    42901 gttttactct cccgtaagcg ctcattactt gcttgtgatc cattgctaaa cattcagcca
    42961 tgtctacaaa atcaacgcct tgcatataat aatgaaaaat tttagcttct atttttgaaa
    43021 actggatttc gcaattttcg attgcttctt ttaataaaag atgctgaatt gctaaaggtt
    43081 cttgcaatgt gatctctgct aaaaagtctg gtccttctgt ctctattttt tggtcgattg
    43141 agacttcatc tttttgatca cgacgcttca gcgcgttctg cttcctcaac agagaataga
    43201 tttgattgct tataacgctt ttataataac ttccaaagct aacacctttg cttttttcgt
    43261 aagttctcaa tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat
    43321 aatttcttaa ataataggtg tttttcaaac gtagtaaaag tggaaaatat ttttgataca
    43381 gggcagcaaa tgcttcttcg tcaccattac gtgcagcgcg caataactcc tcagacataa
    43441 aataattcct ccataaattc gtttatggag gaatcttatc atcaatgatt catcgaactg
    43501 attagaacga tatgttattt attttttctc attaaatcgt ccaatttttg ggacaatttt
    43561 tttaactgct cttcattcca tggagaatta cgtcggaaat caataaaatg catatcttta
    43621 ttttcttggg ctattgtttt ttccgttttt tttatcgtct tatataattc ataagcagaa
    43681 gttcgtaatg ctccttggga aaagatcatc cactgttctg ctaaatcgct tgtcgccact
    43741 gtcacttgtg tcaaacggtc attcagctga ccagcgattc tctcgatata actgtcagcc
    43801 gtttcgtctt ctttcgtaaa aacaacatcc aactggtatt tcttataatt ttgttggatg
    43861 cccggaacta gttgggcatc gaagacaacg atgacttcca ttccttcata tttcgcataa
    43921 tttgataaac gatgtaaaag agcttctctt gcatccgcca ttttgtcctg ctttttaagc
    43981 tggacaagtt ccggccacga accaatcatg ttgtagccgt ctacaatcaa cagctgtttt
    44041 ttcatggtct actccccctg acgttgggaa aaagcctgat acataagcaa accagcagca
    44101 acactggcat ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt
    44161 tcttttttca atcctggact cattcctttg ccttcattac caatgactaa agcaattgat
    44221 cctttggcat tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat
    44281 cctgtttctt tgagctgttg cacagcttgt accaaattcg tcacgcgtgc aacagggaca
    44341 tgttcgactg ccccagtgga agtctttgtt acgataggtg tgattccaac tgctcggtgt
    44401 tttggtatga tcacaccatc gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa
    44461 ttgtgtggat cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt tttctctttt
    44521 gttcgttcaa tcagttggtc aagggataaa tattcataag gtgtaatcgc aagaacgact
    44581 ccttgatgca cgccgtgatc gctcaaagtt tctaattttt gtttaggaac ccatttgact
    44641 ggcacagctt gttcacgtgc gatttctttc agttcttcta ctttttctcc tcgtgaatct
    44701 tcttgaagaa agagtttatt ccctcgccct tgttgtagcg cttctactgc ggcatggtgc
    44761 ccaaagacga aattatcttc taatgcttct tcattttgat cagaaactga ctgatttcgt
    44821 ccagtttgct ttttatttga atatctattt tgttttttgt cttcaaatcc acgtttcgat
    44881 tgatgtttgt ttgttctttc tttacccatt tttttcacca actttctgaa tacaccagcg
    44941 gatcaattct tccatccgct cggtttgttt tgtcaaatgc agatagccca tgagcgcttc
    45001 gaaccctgtt gcgacccgat acgtcgtaac atcagcattt tttgcgctcg tatggctttt
    45061 agcatttctt cctctgcgat aataaagttc ttcttcttct gtcaaaagat gttcttccag
    45121 catttgctga atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg
    45181 cagcttattg ggttttgttt ctccttgatc aatcaggtgg tcacgaacat agatttcgta
    45241 aatcgcatct ccaacataag ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct
    45301 tctcttctcc atcgggttcc ttgcggtgta tcttctaata agatcccttt ttctttcaac
    45361 aaatcacgaa tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg atttcgttct
    45421 tcgatcaatt gttcaatctc ttcatctaat aactcttctg ctgtaaataa aataccgaag
    45481 atcgaaagcc attgcgagaa taaagcttct ccttgtgcca agacagcggc agaaactttc
    45541 ggctggtcta gataagtgtt cagccactta gcaagctcat agaccactgt gatcccattt
    45601 gctgcattaa aatcatcgtc catttcttcg atgaatcgct gttctaaatt ttctaacaaa
    45661 gcgattttat ccgcatcatc tgccaaattc tctgaggcag tttcttggcg gaatttcaaa
    45721 ttgtcaaacg tattcttcag tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca
    45781 ctgtagcgga tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa gacttgtgga
    45841 tcaacgtttt tgatcatttc atgtacagtg atgaaattcc ctagagattt actcattttt
    45901 tcatcgtctt cgccaatcgt tacatagcca ttgtgcatcc agtaattagc gaattttttc
    45961 cctgttttcg cttcactttg tgcgatttca ttttcatgat gaggaaattc taaatcttgc
    46021 ccgccaccat ggatatcgat cgtttctcct aaatgttttg ttgccataac ggaacattca
    46081 atatgccaac cgggacgtcc tttgccccat ggtgaatccc aagaaatctc gccaggtttt
    46141 gcttgtttcc ataaagcgaa atctaatggg tcttcttttt gctgctgttc ttcccctgta
    46201 cgctgacttg ctccgacttc cagttcatcg atggattggt cacttaattt cccataattt
    46261 tcaaatttac gggtacggta atacacatca cctgaagatt cataagcata gcctttttca
    46321 accaacactt gaataaatgc taaaatatct ggcatatgat ccatgactct tggatgcagt
    46381 gtagcaggtt cgacattcaa cgctttcgta tcttcttcaa atgctcggat gaaacgatca
    46441 gcaacttctg gtgctgtaat acctaattcg tttgctgtac ggatgatttt atcgtctaca
    46501 tccgtgaaat tcgatacgaa atcgacttta tagcctcgat attcaaaata gcgtcgaatt
    46561 gtatcgaaag caatggtgct acgagcattt ccgatatgga tataattata cacagtcggt
    46621 ccgcagacat acataccgac atgtcccttt tgtataggga tgaattcttc tttttttctg
    46681 gtcaatgtat tgtagatttt tatcattctg atctcctcac tttttctcct ttgattcgaa
    46741 cgacttttgc tggtactcct accgctgtag cgtcgtctgg aacatctttc aacacaacgg
    46801 cacctgcacc gattttggcg ttttttccga tcgttaccgg tcctaatatc tgcgcattcg
    46861 cagagagcat ggctccttgt ttaacagttg ggtgtctttt acccgtttct tttcctgtgc
    46921 cgcctaatgt tacgccatgg aataaaatca catcgtcctc gatttctgcg gtttcgccaa
    46981 tgacgattcc cattccatgg tcgatgaaca cgccagttcc aatcgttgct cctggatgga
    47041 tctcaacacc agttaggaaa cgccagaatt gagcattgat cttcgccaat aaaaagagcc
    47101 ggtgctgata taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg
    47161 tcaaaacgat ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg
    47221 ctcgggtaaa ccaactcatg actttccact cctttcgtaa gaattttatg taggaaatca
    47281 tcttcttata aagaaaagaa gcgcctttta aataagtttc cttatcaaaa gacgctttcc
    47341 gcgtggttcc acttttcttc ataatcaatg attatctctg acagctaacg cctgttatac
    47401 gttcttggct actttgtttc ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga
    47461 ccactcccag aggcatttcg gcaaccattc attgatacct tgcaccaccc ggtatctctc
    47521 ttgtaatgat cagctgctta cttttttctg ttcatcgtgt ttagttcagt acttgattta
    47581 aatgtgctaa tgatttttca cgaccaagca attcgatcgt ttcaggcaat tctggaccat
    47641 gcatttgtcc tgatacagct acacggattg gcatgaaaag attttttcct tttacgccag
    47701 tttctttttg aacagctttg atggcagctt tgacagttgg agcatctact acttccattt
    47761 gttctagctg tgctttgaat gcgtggagaa cagtaggaac tgtttcacca gcaagtactt
    47821 cttttgctgc ttcattcaat acaggatgtt cattgaaaaa tagcgtagac agatcaacga
    47881 tttctgcagc atagctcatt tgaggttgat acaggctaac gatcttcttc acccattcga
    47941 ttgtcttttc atctgggttt tctgctacac gtccatcagc gattagataa ggcagacaca
    48001 tgtcagtaaa tgcatctaga tccattgctt tgatgtaatg gttgtttacc cattgcagtt
    48061 ttttcgcatc gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac attttgatca
    48121 attcttggtg ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa
    48181 agttgaacat tgcatctggt agatagccta attcgcgata ttgttcgata aattgcaaga
    48241 ttgattcgtc acgtttgctt agttttttac ctgtttcact gttgatgatc aatgtcatat
    48301 gtccgaaggt tggtggtgtc cagccaaatg cttcatagat catcagttgt tttggtgtat
    48361 tggcaatatg gtcatctcca cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg
    48421 caacagcaaa attataagtt ggcataccat cgcgtttttg gatgacaaag tcgccaccga
    48481 tattatcgga ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg tattctgtat
    48541 tgcgaggcac acggaaacgt acaacagatt ctagaccttg tgcttctttt tctgcttgtt
    48601 cttcaggtgt caagttcgca catgttcctg cgtaatgagg catttctcct cttgcgcgtt
    48661 gtgcttcccg ttctgcttct aattcttctt ctgtgcaata gcatttgtat gctcgattgc
    48721 tagcaagcag ctgatcaatc agcggttgat agatttcttt acgttcagat tgacggtaag
    48781 ggccgtattc tccaggattt tcaggagatt catcccaatt cattcccaac caagcaaggt
    48841 tttccaactg gcttttttcg ccatcttcga tattacgttt ttggtcagta tcctcaatac
    48901 gaataataaa gtctccatca ttgtggcggg caaacaaata attgaataat gcagttcttg
    48961 catttccaat atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt actttcgtca
    49021 ttttcttcct cttctttcaa actagacttt gactaacagc aaaaattccg tcagttacgg
    49081 cttctcaaat atcatttatt taaagccact cagcacaaat tttacctttt ctaaggtaaa
    49141 tagtaaagcc aactggttga atttccttac cagttggctg aatttctttt ttattttgat
    49201 tgtttagtct ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt
    49261 ttagcaaaga tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac gacttcgata
    49321 cgttcattca tgtaatgctg tccatcttct acgacaacca tcgtgccgtc atcaaggtag
    49381 gctactcctt gttggcgttc tgtcccggct tttactacta gaacattcat cgtttcacca
    49441 gggatgacga ctggttttac ggcatttgcc aacgcattga tattaagaac aggtacattt
    49501 tggaattcag agactttatt caagttatag tcattggtta caatgacacc atctaaaagc
    49561 ttagccaatt tgatcaattt gctgtctact tcactgatat cttcaaagtc tccatcgtac
    49621 atttcgacag atataccctc ttctttttgc aaggcattta ggatatctaa tccgcgacga
    49681 ccacgcacac gtttcaaact atcccctgag tccgcgatgt attgtagttc gtaaagaaca
    49741 aaattaggga tcatcaaggt tccttctaag aatccagtct ttgtaatatc ataaatccga
    49801 ccgtcaatga tcacgcttgt atccaagatt ttatatttat ggaaattttc tcctgatttt
    49861 cgttctaaca gttgactctc aacttgacta gctgtttctt gctcgatttt tttgcttcgt
    49921 gaaccaaaaa ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag
    49981 cctaagatga tcatcagtaa aatcggcaat acactattga cgaatggaat ttccatattg
    50041 taaagagggg tcgagatcac aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg
    50101 aagagaagat aagtcaagct gacctcactt agtttttgtt ctacttgttt gataatagct
    50161 gagatatgtt tggccaacag caaagataat aaaaagaaaa taagtgcgcc taacaggctg
    50221 ttggtaaatg tattattcag ccatccgttt gactgttgac cgattgcctg ccaagctgag
    50281 ggtaaaaatg aaatacccaa gcttgcgccg acaatgatca tcagcaaagt aatgacacgt
    50341 ttttgcatcg tttttcctcc gtttcgacaa tcatttatta gttagcggaa cacacgcttc
    50401 aacgtttcag ccaaagttgc tactccaact atctcgattc cttttggcgc ttcccagtta
    50461 cctaagttgt ttttaggtag gtatactttt gtgaagccga gtttttggac ttctttgact
    50521 cgttgttcaa tatgactgac acgtctgatt tctccagtca gtccaatttc accgatgaaa
    50581 cattccgttg gctgtgttcc tttttcttta tagcttgaag caatactgac agcgatagct
    50641 aaatcaatcg ctggttcatt taattttacc ccacctgcag cttttaagta agcatcttga
    50701 ttctgaagaa gcaacccagc tcttttttcc agcactgcca tgatcagaga tacccgattg
    50761 aaatcaagac ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt gactaatgct
    50821 tggatctcta ccaaaattgg acgtgtccct tccattgcca caacgatagc agagccagtt
    50881 gcaccgtcta ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc ttccaatcca
    50941 tgctcatgca tttcaaaaat cccaatctca ttcgttgagc caaatcggtt ttttactgct
    51001 cgcaaaatcc gaaaactatg atgttgttca ccttcaaaat ataacaccgt atccaccata
    51061 tgttctaaca ttcttgggcc ggcgatcgag ccttctttgg taacatgacc gacgataaaa
    51121 atggctatcc catttgtttt agcggttttg agtagttctg cagtggtttc tcttacttgg
    51181 ctgacacttc ctgccacact tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg
    51241 ataacataat caggggagat atgttcgatg gcacgactga tttcgttcat atctgtttca
    51301 gcgtatagat aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc
    51361 tctgcgcttt cttcgccgga tacatataag acttttccgc ctatggctgc taattgctga
    51421 gaaacttgta acagcaaagt ggatttcccg atacctggat cgccaccaat caagaccata
    51481 gaccctggca ccacacctcc gcctaacaca cggttcagtt cttctaattt tgttttgatc
    51541 cgaggttctt ttttagggac gacgtctgcg atttttgttg gttttgcttt ttctcctgtc
    51601 aaacttgtcc gagttctgcg gtccgttgta tcttgttcga tttcttcgac catcgtattc
    51661 cattttccgc aatttgggca gcgtcccaag aacttaggcg acacataccc gcaattttgg
    51721 caaacaaatt gtacttttga tttttttgcc attgtaccgc ctccttttat cctaatcatg
    51781 agacattaaa tcaagcttaa gtatctttaa cgttttattt actggaagaa ccaaatccgc
    51841 cttgacgttg agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa aagatccctt
    51901 ggccgatacg ctcgcctttt ttgatcacta catccttgaa accaaagttg gtgaattgga
    51961 acatgatatg gccttcattt gcctcattat tgtaataatc actgtcaatc acacctactc
    52021 cgtttgtcaa cactaaaaac cgcttcaacg gattgcttga acggttagca agctgcaaaa
    52081 attcatcttc tcccatgtaa gctttgattc cagtaggcac gagggttggt ttcaattgtt
    52141 tttcattttc tgatacatct tttttcaatc catctgcgac tgtttccaac ccctttttcc
    52201 agcctgcttt ccagatactt ggaatcacga cctcttctgc tgcttcaaaa tcatagccag
    52261 cagcatgatg tgtagcgcgc tgcggtagat tgatattttg atctttatat ttagaaataa
    52321 cttcaaatcc tctttttctc accggttttc ctccctaaat aaactactcc tattctaaca
    52381 tatccgaggc tgtaaaagac tttctttaca aattcttcaa gaaattctgc tatcttaaag
    52441 taaaccatta attcgttctt tttccaaacg actgcaagtt gtacaaaaaa actatttttt
    52501 acatgacttt actcgtcaaa acaagtagaa tagaacgaaa ttaatgagtg gatatttgat
    52561 tgaggtgtca catgcgtaaa gaaattaaaa atgcaaaacg aattattgtc aaagtaggaa
    52621 caagtacatt gatttatccc aatggaaata tcaatcttgc ggcaatcgat cagttagctt
    52681 ttgtcctttc tgatcttaga aatcggggaa aggaagtgat cttagtctca tcaggagctg
    52741 taggcgttgg tatgcataaa ttacatttgt cagaacgtcc gactttgatt cctgaacagc
    52801 aagctgcggc ggctgttggt caagccgaac tgatgaatat ctataatcaa agattcagtg
    52861 tatataatca acagacggca cagatcctcg tgacaagaga cgtggtagat tatcctgaaa
    52921 gtcgtaaaaa tgtgaggaat acattggaac agcttttgat tatggacacg atccctatcg
    52981 tgaacgaaaa cgatactgta gcagtggatg aattagatca tctgacaaaa tttggcgata
    53041 atgatcagct gtcagccatc atggcaaaac ttgtcgcggc agaccttttg atcgtccttt
    53101 ctgatatcga cgggttttat tcagataatc cgactacaaa tcccgaggct cagatgtatc
    53161 gctatatcga taaaatcgat gaaaagattc tggcacaagc aggtggtgct ggtagcacat
    53221 ttggaacagg cggtatgcag agtaaattaa aagccgcttc taggatattt gaagcaaaca
    53281 gcgcaatgat tttagcaaat ggaaaaaatc cggcaattat ttttgatatt cttgcaggaa
    53341 aagaagtagg tactttattt aaggaggact tgaaatgact gacttgatgc aattaggaca
    53401 acaggcaaaa gatagcgcgc aacaactagc acttatgcct acaaaccgca aaaatgagct
    53461 acttgttcaa atggcacaaa ccatcaaaaa gaatcaacaa gcgatcattg aagcaaatca
    53521 taaagactta gaaaaagcag tcgaaaacaa tatcagtgaa acgatgcaag accgcttgca
    53581 gctgacacct gagcgaatca atgccatggc tacagagatc gaaaaaattg cttcgttaga
    53641 cgatcctatt ggaaaagtag atgagatgtg gacgaacacc gacggattga gaatcggcaa
    53701 aaagcgcgtg ccactaggcg tgatcggcat catttacgaa tctcgtccaa atgtcacgac
    53761 agatgcagct agtctagcat tcaaatcagg gaatgccgtt atccttcgcg gtggcaaaga
    53821 agcattcttc tcgaatcaat tattagtcca attattgcag caagtactgg tcagtgctgg
    53881 cgaatctcct tatgccatcc agtttgtcga tgatacttct catgaaactg caggacagtt
    53941 gatgcgattg acagaatact tagatgtgct gattcctcga ggcggagcaa aattaatcca
    54001 acgagtcaaa gaacaggcaa cggtacctat cattgaaaca ggtacaggaa ataatcacgt
    54061 atatatcgat aaagaagcac agttatctat ggcagtgaac atcatcgtca atgcaaaagc
    54121 ttcccgtcca tctgtctgta acgcggcaga aacattgctg atccattctg agatcgcacc
    54181 ttttttcttg ccagctattg aaaaagaact ggtagaacac ggggtctctt tacgtgcgga
    54241 tgctcgtgcg ttagaatatc tagagacggc tgctttagca gaagacacag attgggatac
    54301 tgaatatcta gattatatcc ttgctgtcaa agtggtcgat tctttggatg aagcaattgc
    54361 acatatcaat cggcataata ctaaacattc agaaacgatc gtgacagata gttatgcggc
    54421 tagtcaacgt ttcttaaatg aagtggacgc ggcggctgtc tatgtcaatg cctccactcg
    54481 ttttacagac ggatcggtat ttggttttgg tgcagagatt ggtatctcta ctcaaaagtt
    54541 gcatgcccgt ggtccaatgg ggttaaatga attaacttcc acaaaatata tcatttatgg
    54601 cgatggacaa atccgcgaat aatgtttcgt gtgaaacatt attcccgcct tttgcctttt
    54661 ttctgtaaag gttctatcca actagaaagg tgtcaatatg tttgaaattt taatgatttt
    54721 ttttatttac cttatttctc tgaatatcgc tgcttttctg ggagtcggta ttttatccct
    54781 tttctttcaa ttcaaaaaac gaagcatagg gagtcaacgg gaaaaatggt cgcagtattt
    54841 tgacaaaatc gggcaaaaag ggctggttac acgcttgcac attagttaca tggttgcatt
    54901 atgcttatta gcgacaatca attactattc ttttttcgat cattcaatcg cttatacgat
    54961 tacgctactg attgctggga tttttcactt atcttataaa tatcagctga ataaaaacca
    55021 cttgaatcgg actttccgct aaaaaatatc cgtacgataa gtagagtgcc ttgaagatcg
    55081 gcaaaaaact gccggacttt ttagaggctg tgacaaagat tttgtcacag cctctttttc
    55141 catataaacg gcgttcgcct accagctgcc tggtaaatct gtttcctctt cgttaagaag
    55201 cgaaaatccg tcaaatgttt cgccatcata ataacgaagc agctcatcca tggacaagtt
    55261 catcgttttt tctttttctt cttccgctga ttcagccagt agctgttccc gatacacctg
    55321 atacatacca gctatttttt gctgcatcgc ttttctagtt tcaccttccg cattgattat
    55381 tggcaattct ttgatatagc cacgataaat caaccgatca tctgaaaatt caatgggtaa
    55441 ttcaacgagt gtcaacccga cttcctgaaa atattcttcc agcatctatt atccccccat
    55501 tctttttctt aaatgaattt tagcttacct actgaaaatt ttccagtttc attattttga
    55561 gaaaaaaagg gaatgtgaca aaaatcgtgt cacatcccct ttttccgaat aaatggtgtt
    55621 cttctgccaa ctcctcaatc ataagtcaca atattcaaca aacatgagaa actgtttttt
    55681 gaaattgttc cttgtgattc agagttaaac gacagtctca caacctcttg gtaaacggcg
    55741 ttcaatcagc tgaccctcgg tattagctca aaaaacggta aaatatgaga agctattttt
    55801 ccgttttgtt tctctaatac ttaggataaa acgctgattt tacaacctct gttaaacggt
    55861 gttcaatcag ctgtccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt
    55921 tccgtttgtt tctctaatac tcaggacaaa acgctgattt tacaacctcc tgataaacgg
    55981 tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat gagaagctat
    56041 ttttgtttgg ctgttcttat gacttgatgc aggacagctt tttcacaacc tctgatttct
    56101 tattcttctt catttgtagc aaaataatta cggtgataaa tgatacaaca tacactgata
    56161 agaacaatgg cacctgcaaa ataaaacgca tagctttcac caatatgttc accaatcaca
    56221 cctgcaataa atggtgctaa agctccaccg aaaaagcgga caaaactata gctggacgaa
    56281 gcaacgtttc gttgcagacc tggtatttcc atagcaatcg tcgtcaacag tgtattgata
    56341 attccttgga aaaatcctgc taacacaatg ctgatcggta caaggactgg catagaagtt
    56401 ccagctccta ataacagcaa acaaataatg aataagacaa atgctgctaa aattgtccga
    56461 taagtcgtaa acttccgctc aagagccggt gcaacgaaaa tcgaagcaat agccagaagc
    56521 acaccccagc caaaaaatac aaaaccgact tctaattcag aatagccaat caataaaaat
    56581 ggcgcatacg caagtaaagt aaagaatccg aaattatata acaatgcgat tagtccaact
    56641 gaacgaagtt tatgattgct taacgctttg acaccttccc aaaggcctac ttttctcttt
    56701 ggtttctcag tttcaggtaa aagtaccgct agggctataa aaccgcaaaa catcaaaaat
    56761 gctaccccaa aaaatggata acgccatgaa ccactgccca agaccccacc gactagaggg
    56821 ccaacagcca ttccgcagcc aagtgctgct tcatacatca tgattgcttt ttctgtgtta
    56881 ccaactaaaa tactgacgat cgttgctaaa gcagtagaga caaataaagc attcccgaca
    56941 ccccagccgg cacgtaaccc aatcagcatt ccgatattcc cagataatcc gccaagacca
    57001 gcgaatacaa cgatgataaa caatcctgtc atcaatgttt ttttcaaacc gatccgacta
    57061 gataagaaac ctgtaaatag catcaccaca ccagtgacta acatgtaact agtaaataac
    57121 agggtcgttt cagctggcgt agcgtttaac tgtgcagcaa tcgtttttaa gattgggtct
    57181 actagaccca ctcccataaa agcgatcata caggtagctg ctacaactag cgctgaactt
    57241 ttcccctttg tttcttcatt tgtttgttct tgtatatcta tttgattctc caatatttcc
    57301 atccctttct ctctattagc atcatacatg atttatttta catgtcaata tgcacatgta
    57361 aaacgtggaa actttactgt gatataataa agaaaataag acgagaggta ggatttttat
    57421 gaatacttta gcctatatat tattatgcgt gcttttatcc aaaccacgtt ctgggtatga
    57481 attgaagcag ctgatcacaa ttttttggga agcccatcac agtcagatct atacaacgct
    57541 tgccaagtta gaccagcagg gttatgtgac cgttttggac aaagacgagc actctcaaaa
    57601 aaagatctac cacttgactg atgaaggacg tgctcttgta gaggaatgga tcaaagagga
    57661 aactcccgct cctacgcaaa aagatgaatt tctagcaaaa atctatgctt tttcgacgct
    57721 cgataaaaca acagctactg gactgctatt cactagagaa caacagctga acaaaatcat
    57781 gcaaaaaaat caaaaaaaat tgaatgacct ctcgctagac aagaaacaag aatttggacg
    57841 ttatgttgtg atccagcgca gaattatgct ttgtcagcag gaactccaat ggtgtcagca
    57901 agtaagagaa caaatgcagc agttttttga gtaagggata aacagaacct ttctagaaat
    57961 atctattgaa gagcaaatta tcttcaataa aagagcaata cagttggaaa agaaaaagcg
    58021 atccgaacga caggccaaaa cctatcgttc ggatcactat ttttctagaa ctgcattcaa
    58081 gagactatga caaaaagcat gccaaggcct tttttcaaat aacagtgttc catcattcat
    58141 tactttgaat tgcttttagc acacttcttg aaagcgggcc aacgatgatc aactgcaacg
    58201 gtaaagcggc aatcacattc attttccaag cattcatata agcagagaat aattgatcag
    58261 gtattccttg ttccatgatc acaccgaata aagacataca tgtcaccatt cctaaaacca
    58321 tcaagcatga aatagttaag atcatttgga ccttgctttc tggatttact ggtagtttaa
    58381 aggcaatttt tttcgccagc acaccgatga tgaagacatc caaaatgaaa gccaccacga
    58441 atccaggcac tagaccaatc aacaaacttg ataaatgaag atcttgatgc agccataaat
    58501 tatacgcgct catccccaag accatcaaaa aacacatcat tgttgtaaaa attattcctt
    58561 cttttttatt tgtaggcaat ctgtaccact cctttaaatt tgtcacgaaa atcatcgtag
    58621 cacatttaaa aatttctcgt aagtactttt tttattttct gcccttttat ttcataaact
    58681 tcatcagccg tacgctcgac aaagtaagaa tcatgagaag taaacaaaat cgttcccgga
    58741 tatatttgca ttagcttctc taaagcttcg atcgttgcca tatcaataaa attagtcggt
    58801 tcatctaaca gcaacacatt acttggctta gtaaaaagca aagcaatcgt cagacgggtc
    58861 gcttctcccc cacttaatgt acataacggt ttcgtcaaag tttcctgagc aaaccctaag
    58921 tgattcagca aactacggac aagcgattct gaaaactctg tttgtctcat gacaaaacga
    58981 atgatggatt cttcagatgt catttgataa cccttttgct gatatacttg aaagcttact
    59041 ttcggagaga gaaggattcc ttctccttgt ttgcggatat gttctaataa ggttgtctta
    59101 cctgaaccat tttcaccgac aatcgcgatt tttttaccta aagaaaactg aaaatcacca
    59161 tttaccagta atgttctccc acttctttcc aattggacat tttgtcccat gatgggatag
    59221 cggctgtgga gttcaagaga ttttggtact ggaaaacgga tttgtttcat ctgctgcggc
    59281 ttggttgttt ctcctatttt ttggagtctt ctctctaatg ttttcgcatt cttttggatg
    59341 gctttttgta cactgtcttt ttgtttagag gaagacaacc gatccagtct gatttgttgc
    59401 tttttctttt tactggacac tttttccaac ttttccgctt gttttttctt ttcttggatc
    59461 gatttttcca atcgtttttt ctctttcatg atcgactctg cttctcgtag ctgagtcttt
    59521 ttctccaatt ccttttgagt gagataggca ctataattcc ccgaaaactc tcggatttct
    59581 ccgtctttta cttcccagat tttcgatgct agctcatcta gaaaatagcg atcatgacta
    59641 acaaaaacca gcgtaccata ataataacga agctcttcaa tcagtcgatc aatacttttt
    59701 ctatctagat gggttgttgg ctcgtctaga agcatccctt gttcataaga agaaagaaga
    59761 cgtatcaact cgatctttcg ttcttcccct ccgctccatt tccgctctga agtttcttcc
    59821 agtcttaact ggaaatgact ggctaattcc aaatttaggt cctttgcttc agcgagatag
    59881 gttaattgag gcaggtagtt gaaggtgatc tctgtttgga ttttcccatg atcgggaaaa
    59941 agttcaccgg ctattagttt gagcaaagtc gatttaccga caccattttc tcctatgatc
    60001 cctatacgat cttgctgata gacacgaaga gaatcaatag atagttctag ctggttgcca
    60061 aagctgactt gtagatttgt tatatttact gctaaatttt ccatatgttt tacactccta
    60121 tttcatgtaa aacaacaatt atcgatacta aaaaagagca gaaaaatctg tttgcaaaaa
    60181 aactgcatca catcttttcc tactctgaac gaaataacta tgactaaatc accaaatctg
    60241 tcatagcttt cacctattct actgtcgttc gaaaaaatgg acatactcca tccatgaaat
    60301 tgttgtttat acatgtcttt tgaatttatt tagttgttat tcaaaagttc gaaacgtaat
    60361 ttcatcgatg cagtcatttt tccgacctcc ttttttatta ggttaccaca cacgattcaa
    60421 aaaaagcaat agctactgaa tagtaactga aaacctttta ttcgaacata gtgatctttt
    60481 ctggtgctaa acgaacagac tcgtaacctt cttctaccgc ttttcctatt gataaaataa
    60541 taactggaac ataacggtct ttatccaaac caaatgcctc tgccaactga tctgcttcga
    60601 atccaccaat tggatttgtt tcatagccat gatcacgcgc aactaacatg aattgcatag
    60661 ccgctaagct agaatcgact ttgactacat cgttcatttc ttctcttgaa aaactcttgt
    60721 aataaggaat gatcgcagct aattgttgat ctctgacttc ttttggcatc ttccctgatt
    60781 cgtaagcttg gttgtagatt tcttcgccat attcgtaaca ttgcatatcg ccaaaaatca
    60841 tgaccattgc agaagaagta tcattttgtc tcgtattgaa acggatcaaa ggacgcaaga
    60901 tttcttttgc ttcctcgctt tcagcgacca caaagcgcca tggtttcata ttgacagaag
    60961 aaggtgcagt cgtcgcttct ttgatcattt ccagcatttc ttcgtgtggg attttgtatg
    61021 tttcatcata tacgcgaacg gatcttctac cagtcataat tgttgaaaaa tcattttttt
    61081 gttccattat tattaacctc tttcgtttct gttgttatat tgcaaagttg ggtattcaat
    61141 ttatctaaaa gtgttagaaa ttgtttcatt tctacatcgc tgaaagccgg ggatagcacg
    61201 gaacctgtat ctttatggtc catttcacaa tgcttgattt tcttctgggc tttttctgtt
    61261 agcgctacat acacttcccg gttgttctga ggatttctct gacgcgaaac atagccattt
    61321 gcttccaata ccttcagatg acgagtaact gctccttgat caatctgcaa cttttcttgc
    61381 aaactgcttt gtagcaatgg attttcttct ttcagtctca tcatgatctc gtatctagta
    61441 agacttatac cgatattttc ttcaaagatc tggacgattt tttgatcggc taatttgatt
    61501 tgatataaaa ggtgactaat ctcttctaaa gacataattt cctcctttct aataattgac
    61561 tcgtcaacga ttgacttgtc aataatagaa taaaaaaaga aaaaagtcaa agaatcagct
    61621 gtgtaataaa aaaaacgctc tcaatagaaa agtatagaga aaagaaattt tttgtagaat
    61681 gaacggaaaa aatgataaag aaagcacaac gctaaaacca agattattga ttcttatcaa
    61741 actatagaag atagctcttc tgcagtagat aattttacac cgccaaaaat ttccgaaatc
    61801 gccttttgca agaatcccgt cgaatattag aaattggatc agtatgctta aacagtaata
    61861 aaaaagttat gacaagatgt tgtcacaacc ttttttgagt aaacggtgtt caaaaacttg
    61921 catctgcggt aataagttca accaaacaaa aaacatgaaa atctgttttt gtttggctgt
    61981 tcttactact tgatgcaggg cagctttttc actacctctt tttaataacc ataaaatcct
    62041 attccttcat atctccagcc catcggtgtt agccaatctc tttccttagc actcaaggta
    62101 tagtgatggg taccagtttt ttcatttgga ttaaatagtc gataaattgg cttggaacca
    62161 ccagaataaa aacttagacc ttcataacgc caaccgattt ttgttaaaga gttgatctca
    62221 gattgtgcca ttgtgtaatg atggtctcca ctgtaaggat tgtataaacg gtagacaggt
    62281 tgtccgctgt ttggtgcttg ccaagcaatc ccttcatatt cccagcctac agttggcaga
    62341 taattttttt ctccggcatt caatgtatat aaatgttctc ctgtatgacg gttatacaga
    62401 cgatacatcg gagaagttga agttggtgct gtatcatact gtgtcagatt ataggtttcg
    62461 attacattgt tcaatgtaga accataatta ggcgcagtgg cataacgacc tgttagccaa
    62521 gccgtagcat ctttgtaaga gttcgtattg cttttccatg caccggaata gtagtaaacg
    62581 cctggctgaa aagaagtcgt cttcaatacc gttacgttat cttgcaatga ttctttgtac
    62641 gaaggatatt ttctaaaagc ggcattcacc gtaatccatt gcccattcac atattccgaa
    62701 gtaggcatcg taacagattg gccattatag cttcctttaa tcccaaatag attgtaatta
    62761 ggggcagaag ccaaagtact gttcccccag ccactttcaa cgatggcttg agcaatcatg
    62821 actgatgcat ataagtcatt ggcacttgcc aaactttgtg cagttgaggc aatactatca
    62881 atgaacgctt gttgagaatt agcagatgag cgagcataaa aagcatagcc agtatagttt
    62941 tcgaattcag acaaatctgc ttgatgggta tgtgtcaatg tttgcccatc tgtcaattgg
    63001 ttcgtgattt cttctgcatt ctctggattg ttctgagctt ctgctgatgt actgtcttcg
    63061 gtttgtgttt gttctgttgt ttcagttgtt ggttcttcgg ttgaaattgt attttctgag
    63121 acagtagctg actgcaaagt actttctgta tcgttttcaa ttcctgtttc tgaagaactc
    63181 gatgtctgag ttgcgacagt ttctgtcgat tcttcagcta gcactgttgt gttggcaact
    63241 gctagatttc ctaaaagaat aacgctaaat aataaattct ttgctttcat cttttttctc
    63301 ctttgaatta tgaaactttt cctaaataac agtgtaacta aaaaagtaaa agcaagtcaa
    63361 tgtccatatt taaataacat ttgtttcttt ttaataaatt tgctttttct gcataaaaag
    63421 acgtaaatga agaatattct tctccattca cgccttaaat tgatggtcgt ttgaataact
    63481 atttaacaag taattctctc tggatattat ccgttatgga ttatacttcc tgtgtcgtcg
    63541 gacggataat catgttgctg acagacatcc ggtctggcgt ttctatcgca aatgctacag
    63601 cagaagctat atcttcagat gttaatcccc attctttttg cgcttcatga agttcaagag
    63661 cagccttttt gtcgctgatg gtttgatata attcagtttg aacagctcct ggagagatga
    63721 tcgtagattt gatgttattt tgccgttgct cttgacgtaa tccttccatg attgcacgga
    63781 cagcaaattt tgttccgcaa taaacggcgg aatcaggata aacaacatgt cccgccacag
    63841 aatcagttga aatgatatgc ccagattttt gtttttccat gattggaagt acggcggaaa
    63901 ttccatttaa cacacccatg atgttgatat caagcatatt ttgccattca tcacggtgac
    63961 cttcaactaa tggagcggtt ggcatgattc ctgcattatt gtacaataca tcgattcgac
    64021 cgaacttttc catcgctaaa tcgataactg cttggacttg tgcaaaatct cttacatctg
    64081 ctgtttgaat atatagctca gcttctggta aagattcttt tattgctttt aggcgatctt
    64141 ctcggcgtgc agctataact agtttagccc ctttttctgc taatagcttc gttgttgctt
    64201 caccgattcc gcttgaagcg cccatgatga caatcacttt ttctgataaa tcattcattc
    64261 tattctcctc ctgttttgaa aaatctcttg cttatactca ctatctctta tttcatctta
    64321 ctgttcaaat aatccgttcg acaggtttga cgattttggc agggattcct gcaacgatgg
    64381 tattgtctgg gacatctttc gtaacagttg cattcgctgc aacaatggca ttctcgccaa
    64441 tcgttacgcc tggcagaata gttgctcctg ctccgatcca agcatttttc ttgatgtgga
    64501 ttggctttac tgacaagcct cttcgttctt ttggatcggt tagatggttc accgtgatga
    64561 ggttgacacg aggaccaatc aatactgaat cttctaaaca gatgccacct aagtcaacga
    64621 acatcacttg acgattgata aagacatttt tccctatact aatatgtttg ccgaaatctg
    64681 tataaaaagg cagcagaaca gtcaccgtat catctacttt gtcactaatg atattttcta
    64741 gccgtttgcg gatatctgct ggttcatggt acccagtatt cagcgctgct aaaggctctt
    64801 cgttttccgc ttggatctga tggatcactt caaagagcgg atccgttact cgaatctctt
    64861 tattgacgat ttgtttttcg atgaggcttg tcattttgct ccctcctttt atccttactt
    64921 tataacggac ggcttatgat gtctaatact tataaaacat acttaactat tccttttaac
    64981 tatacttagt ggtatgcttt tttaaaaaca ggagggaaaa aatggatatt cgtatactga
    65041 cgtatttttt agcagtagct agagaaaaaa atatcagccg agcagcaaaa tctctgcata
    65101 tcacgcaacc tactctctca aaacaactga aagatttaga agaagaactt ggtgtgacac
    65161 ttttcactcg aggctcccga gagatacagt tgacggattc tggtcgatat ctttattcaa
    65221 aaggaaaaga aatcttgtcg ttggtagaaa aaacggcgaa caatttaaca aatgacgcaa
    65281 tcatctctgg agaaatctac attggcggag gcgaaactaa agcaatctct ttcatttctg
    65341 atagccttca ttcgttgatc aaggcgcatc ctgatatcca gtttcattta tatagcggga
    65401 atgcagatga tatcatagac aaactggata gcggattact tgattttgga ctcgtaatcg
    65461 aaccgacaaa caaacaaaaa taccagtcat ttcgactgcc agcccaagat cgttggggac
    65521 ttttgataca tcggaatcat cctttagcaa agcaaacagt catccatccg caggaactga
    65581 aaactgttcc tttgatcatt tcccggcaaa cgtcagtgga cagtcaacta gctgaatggc
    65641 taggtttcag catagatcag ttatcgatcg tcggaacata taacttattg tacaatgctt
    65701 ctttgatggt cgataacggg acttttggtg cattagctct agatgggatc atcaatactt
    65761 atgggacgga aaaagtattc atcccactcg atccgccatt acaagcttca ctgaatctca
    65821 tttggaaaaa agaccagcct ctttctcctg ctgctcgtaa atttttagat gtcctaacgg
    65881 agaaatacag ttaagtagag tttttttcct tcgctttcag ctttttaaca acgaataagt
    65941 taagcaaaag atccaatgat taaataacat aagtttctta atttgttcct tccttttatt
    66001 tctgataatt ccagtaaatt actataatta taaaaaaaga attgacaaaa cataactgtc
    66061 tatataaaat tgattacgtt aacaataatt cgtttcatgt gactagatca tactaggcat
    66121 gaaaaatctc tgaggaacat ggtttcccta ggaggttttt cgtgtctatt tttgtttggc
    66181 cgcaatcaac tggtctagca aacgtgaaaa ccaaatcttc aaaaagggtg cgcgcatgaa
    66241 aatcaaaaag atctttaatc aaaatgcagt attggtagat gacggtggac aggaaaaagt
    66301 agctattgga aaagggattg gatttgacag aaaaagaaat gatttgattt ttgatcgtga
    66361 tgtcgaacgg atatttgtca tggaaccaga aggtcaaatc aagttgcaaa atttacttaa
    66421 tcagattgat gaacgctttt tatttgcagc agaaacgatc attgatcatg cggaaactgt
    66481 tttgatggaa aagttgaatg aacatgtatt gatcgctttg accgaccaca ttgctttttc
    66541 tgcggagaac attaataacg gtatcgtcat ccgcaacaaa cttttaagag aaatcgaagt
    66601 gctttacagt gatgaattca gtattgctca atgggcagta gagtatctga caaaagagct
    66661 tgggattcca tatacttatg acgaagcagg atatatcgca atccatattc acagcggacg
    66721 aagcggtcgg aacaataacc atcgcagtat ccgtgaagtg acgattgtat ctgacatcat
    66781 ccatttagtg gaatcagaat tggaaacaga tatccattca gaaccatttt cactgaatta
    66841 ttcaagactg gtgaatcatt tacgactgct tctacaacga acccatgcgc aacaatatgc
    66901 tgttttagac acggaaatcg tagaaatggt caaacgaaaa tacccagaaa gctataagat
    66961 ttctaaaaaa atccgtgtgt tgctgaccaa agaataccag ctcgcgatca cgaaagaaga
    67021 actgggttac ttggccatcc atatcgagcg tttaagaagc gcaatcgtac aaacaaatgt
    67081 taacaatcat gaggaggaaa aaaattgaaa gcatatctac aacgaatggg acgttccctt
    67141 caacttccag ttgcggtctt acctgctgct gctttactag tagggatcgg aaactggtgg
    67201 gcatcttata gtaacgacat tgtcgctcat tttttacaag ctggcggtaa cgcagtttta
    67261 ggtcaattac ctttgctgtt tgccgttggg ttagcactag ggatgtcagt ggacaaatca
    67321 ggtgctgctg ccttggcagg tttggtcgca tttgaagttc cagtaaatgt tttaaaaact
    67381 gattcagtag ctactttatt gaatatcaaa ccagaatcag ttgatccatc ttttgctcag
    67441 atcagtaacg tatttattgg tattattgcc ggattgattg cggcagctct ttataatcgt
    67501 ttccacgaag caaaattacc gatggcttta tctttcttta gcggcaagcg tctagtgcca
    67561 atcttggctg cctttgtcat gttgctgatc agtgcagtgc tactgctcgt atggcctccg
    67621 gtttactctg ctttagtttc ttttgctaaa ttcattttga gtttaggctg gggtggtgca
    67681 ggtctttatg gcttcttcaa ccgtttactg atccctacag gattgcatca agcaatgaac
    67741 tccgtgttct ggtttgacgt agcaggaatc aatgatattg gtaaattttt ggcttctgaa
    67801 ggaacaaaag gcgtaacagg aatgtatcaa gctggatttt tcccaatcat gatgttcggg
    67861 cttccagcag gagcatatgc tatttacaga aatgcacgtc cagaaaacaa agcaaaaaca
    67921 gcttccttga tgttagcagc agcttttgct tcattcttta ctggtgtaac agaaccactt
    67981 gaattctcat ttatgttcgt tgcttggcca ttatatgtct tgcatgcaat tttcactggg
    68041 atttctttag cagtgagtgc attcttccat tggacagcag gatttgcctt tagtgccgga
    68101 tttgtcgatt acttcctatc tttgaaaaat ccgattgcga atcagccatt gatgttgatc
    68161 gtccaaggat tagtaacagc agtgatttac tactttggct ttaactttgc tatcaaaaaa
    68221 ttccatctga tgactcctgg ccgcgaggaa gcagatcttt cagatgacga tactgcaacg
    68281 actaattcag acaataaata tgcggcacaa gcaagcaaaa tctacgctgc attaggcgga
    68341 gccgacaatg taacttcgat cgataactgt actacacgcc ttcgtttgca agtcaaagat
    68401 acaggcctga tcgatcaaaa taaaatcaaa gccactggcg tacctggtat gaagatcatc
    68461 gacggaaaaa atgcgcaagt catcgtaggt acagaagtac aattcgttgc agatgagatg
    68521 gcaaaactac atggtggtgc tgcaacacga cctgcacaaa caaacaccgt ggttaaaaca
    68581 gaaacatttg ccggtggaac gatcgaacaa gatatttacg ctatcgcaaa tgggaaactg
    68641 attccaatca ctgaagtttc agatgatgta ttcgcggaaa aaatgatggg tgacggctat
    68701 gctgtattac cagaaaatgg agaaatcttc tctccaatcg ctggtacgat cacaaacatt
    68761 ttcccaacga aacatgctgt cggaatccaa acagatgctg gtatcgaagt cttgcttcat
    68821 atgggcatca acactgttga cttaaaaggc gaaccattca ctttatatgt aaaagaaggt
    68881 caaaaagtcg caagaggtca attgattgct ttagttgatc ttgccgctat tcaatctgca
    68941 ggcaaaaaca cggatatggt cgttgtattc acaaatggcg acaaagtcca aagcttagaa
    69001 atcgaacccg cacgtgatgt aaaagcaaat gataaaatcg gctctgtttc caacaaagcc
    69061 tgagccatcg tcttccccag acttttttat cgataaacaa acaggagctg agacaaaaat
    69121 cttgtctcag ctcctttttc cgaataaaaa cggcgttctc ctgccaactc cgcggtaata
    69181 agtccagcca aacgtaacat atgaagagct attttttatt tgactcttct tattacttga
    69241 tgcagaacag cttttttaca acctctgata aacggtgttc aaaaaccagt ttatcggtat
    69301 gaagtcaaat ttatgcaaaa atgtggaaag ccattttcac aaaatttttc ttcatactca
    69361 aaactaatcg gctttttcac aacctcttta tacattattc ctgatccaat atttctttta
    69421 atgctgcttc aagagaagtt aatggttttc ccaatacttt ttctaaatca tcagaggaaa
    69481 tagctaactg atccttttta atatcatgtt ggatcgctaa aaacatgtca gctgcttctt
    69541 gagggaatcc ggcttctttt agattttcga caaacgctgc atcacttgct tcaataattt
    69601 ctaacttttt acctgtcgca cgctctaatg ccttggctaa ttcttcatag tcagttagat
    69661 tgccggataa ttctaagatt tctggagagt cactttctac tagtactttt gctgcagctt
    69721 ctgcatattc acgtttcaat gcccaacctg tttttccatt tcctgcagca taaacgaatt
    69781 gaccagtttt taaagcctgc ccaatgatag gtaattcatt ctctacatac caattattcc
    69841 gtaaaaatgt atgggaaata cctgaatcct taatcatttt ttcagtgaat tggtgatctg
    69901 gcgctaatac actgatcgag tgatcggcat ctgcaaaact tgtataagcg atataagata
    69961 ccccagctgc ttgtgctgcc ttcactacat tcttatgttc ggcttgacga tttcctggaa
    70021 cgccagagac aaaaagcaat cgatctattc ctttcaacgc ttctttcatt gagtctaaat
    70081 catcataatc accaatacgt atattaaatc cagcttcttt cagtgcttct cctttttctt
    70141 cactacgtac tagtacatag atttcatttt gtggaaccat cttttttaag aattccagcg
    70201 cataaccacc aaaacctcca gttgctcctg ttactaaata atgcatattt gccactccct
    70261 attcttttta tttgttaaga tttacagctg tacatttata aattacacct ttatcataaa
    70321 gagtgtggta agataagtca agcaaaaaaa atgaaaggat gacaactgat gaagtattct
    70381 ctgcaattta gtgatgcaat ccatgtactt gcctatattg aaatgtttca agatacccga
    70441 tcactttcta gtgagatgat tgccagtagt atcgaaacaa atgcagcaaa tgtacgtaaa
    70501 atcatgagtc agctcaaaaa atccggtttg atctttacaa aaaccggaaa accgcagcct
    70561 tcccttgcca aaagtccaga tcagatttct ttactagatg tctatcgcag catcgaaggt
    70621 aatacacagc ttatccaagt cgatccaaaa acaaatccaa attgcgtcgt tggtgcgaat
    70681 atccaagaag ctttagaaga aagctacgct cggctgcaga aaaaggcaga agaagaaatg
    70741 gctaagatca cacttgatag tctgatacat aaaatagccg ttttggaaaa agaaaaaaga
    70801 ccagaaaaca caggcctcgt agagagattt ttataattct ctctactgtg cctgcatttt
    70861 tctgatcgga atcgtctaca taaattcttt tattctccgg gggacaaaag ccttgtcatg
    70921 cccctttttc ctctctaatg ctcaggtcaa agcgctgaag tcatgacgtc ttgataaacg
    70981 gtgttcaagt agctgaccct cggcattagt tctgaaaaat gcaaaatatg gaaagctatt
    71041 tcccattttt cctctctaat gctcaggtca aagcgctact ttcacgacct ctatattatc
    71101 atggtttatt gactaccggt atccagattt ctcccatata tttcccgttg gtaactccca
    71161 tttcgactac agcatttgga cccccaacat aggcgtaatc ttctacttct cttaaaacat
    71221 gcccaaaggc cgtattcgtc aaatcatttc ttaattcttc aggcgattct gctaaagcga
    71281 ttacaacaag atacgttcct tttgggaatg ggatgattcg ggtagtgttt ggaacatcct
    71341 gactcgtttg aacaccgaca tagtacatca ttttcccgtt atagggttcg ttgactgtaa
    71401 agaaataatg attttcagca gcttgtttca atgcatctat tgttccatta ccgataactt
    71461 tttgctcaaa tgcttgtttc tctgcagcca aaccgacaaa atccgtattg ctactcttca
    71521 attccacacc aatacccgtc accgtaaaat ctggtttttc ttcgatccta tactttgcca
    71581 tttctcccac cctttctatt atattaagtt catcttacat aaaattaaag ggaaatgaca
    71641 cgattacgct cttttttcta gggatatttt tattttttaa ctattttttt gctttcactg
    71701 caatccaaat ctcactgtaa acattactga aatcctctcc ccattttgta aaagagatct
    71761 cgggagcttc gactaactca taatctgaag aaggaagcca ttctgcatag attttccccc
    71821 aagtatcttg taaagtttgg ggaaatggcc ctttattagg aaaaatggcc caagtgtgtt
    71881 cttctatagc aagcatttct aaatcagcgt acggattttc ttttgtcgta gcaaagccaa
    71941 tcatgtgcat caaactgccc ttttcttcca agcgggctcc atcaaatcca taagaaacgt
    72001 tgattacttg atttggatat aaatctccta acttatgcat ctcttccctt tgagtctccg
    72061 tgatcgtctg cgctaacttt tgtatttctt gattgactcc ttcaaatttg acaggaactt
    72121 ttttcgccac acctgccaca taaaactgtt ctttcttttc gatacgaaat tccatcgatt
    72181 ttcctcctct gacatcaata aaaaatgtca agcgtggaaa agatttttgt atgccgtatc
    72241 tgctgatttc tgaaggcaga tatcctgacc aatcacgaaa agctcttgaa aaaccttcga
    72301 ctgtttcata tccatatcta aacgccgttt ctgtcactct tgatccatct aggagatcgc
    72361 gattagccaa agccaatcta cggtttttga tatagtccat tagcgacatc cctgcaataa
    72421 acgtaaagat gcgttttaat tgatattcag agattcctgt attttcggct attattttat
    72481 aggaaagtcc ttctgtcaaa tgttcttcga tgtaatcgat caattggttc agatctttaa
    72541 gcatatttat cttcctcctt gcttgccttt ttatcatatt atcaaagaaa gtcaattact
    72601 tgatgaatca ggaaccaatt tagtcagatg tagaaaatgt tgtatggatc taaagaaaat
    72661 tctcaagcag cgtttcgtaa cggataattg gtattatctg cctatctatg ttttaccttt
    72721 agaaaaataa attttgggga agacacattc taagtaaaca aagcggtaaa aatatctttt
    72781 attcccttag ttaatccgct tactacctac tgtacaaagc aaattgttgt tcaaactgct
    72841 gatcagaacg ctgcttggat ttttctttgc gatacatcat acctctttta tcaattggaa
    72901 caattattta tttctaacaa aatcacttca tttttgtcag ataaataaaa aacaaaaaaa
    72961 gttccatctt taaaacacgc tattctttta ctttttctca cattctctaa gaaaagaaaa
    73021 ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc aaatatgtaa aatcagctac
    73081 ttttttctta aacgtttctt tatactaaat ataaataagt gtttcatgaa aggagaacta
    73141 gatgaataat tggcttgagt attttcctga aaacgttttg gaacgcggtt acagctatca
    73201 tctgcacggt tttgtccgtc atttgaacta tacaagcaag tacttgtccg caacagtttc
    73261 tggcacggaa gattataagg tagtgatcac ttgggatgaa aagaccaaca tgacttgtga
    73321 ttgtctatat gccatagaag gaaaaaaatg caaacacatg gctgctgtct tattcgctta
    73381 cgaagaacgt ccaataaaaa aatccaacta ctcactttcc gaactctcgt cccttgtttc
    73441 ctccgctagt tcctctcttg tacgggagct tttaacagag attcttatag aacatccgca
    73501 atttatcgaa cgcttcaaag ttaagatgcc atttcatgca ataaattatt cagataaatt
    73561 aacaactatc atccataaat acgatcatat tatcaagaaa aacaaaaaca ggaaaacagc
    73621 gaagttcatc atggagatgc gaaagttcat ccaagaagca gttgaatcgc tcattcagca
    73681 aaatgcctat cttcccgcat tcgaattgat caatgaagtg attgctactc tcgaaacatt
    73741 ctattgggag cctgaggatg aacgaaccct tcttctgata gaggactgtt attatttatg
    73801 gaaagaatta ttagcagaag cacctcatgc agaaaaaagg caaatgttct cttggttcgt
    73861 ctgccaagtc gatcatactg acgcctctta ttcaaaacgc tactccataa aaatcctgaa
    73921 agaagacttt cgggaaaaag aattttctaa ccaaaagaaa aaaatagata aaaaaactaa
    73981 agaaacggta aagaaagacg atttcaatga aaaatagcaa gtaaacgata acaaccatcc
    74041 taaatccaca agaaatacag cagaccttgc tttaaaaaca cgaatcatgc aaaggatgaa
    74101 gtaaagaaga cttaacgcgt acccaatatt agtcatttga ctattaggta cgcgttttgt
    74161 tttataagtc atgacaaatt tccttcgttg ctgggtagat tttccggcat tgttgataga
    74221 ttttatgata cttttctaca ttttgtaaga gaggttccac tgcatctgta tattggataa
    74281 aattctgaca acaactttca ggtgtgtcga accactctaa tctttaaaga taaatattaa
    74341 aataacgatt aataatatta acagtcttta tttttcacca ccaaaaacct atctttgcat
    74401 atactaaata gacgtatata cacagatagg tttgtcttgt gaaaaataat gattatcctt
    74461 accaaaaaat gtttgttttt ctacatgttc gtacttatca aaaaatatga tccttattga
    74521 aattactggc tatttatatt ccaacgactt cttgtgttaa tttttgttat ctcatagtaa
    74581 attatgtttc attttcactt aatttagttg tcacgtgttc gtcaattcgt aaaaacggta
    74641 aatatataaa cacacttgcg gctataatta atatttgtaa aacagctgct cgccagtctc
    74701 cggctgttgc taaaaatccc gatataactg gtggcgtagt ccaaggaatt tgcactacta
    74761 catgattaat taatcccaca gcagttgcaa aatatgctac tgtcaaagaa aaaatcggtg
    74821 caagcacaaa gggaataatc agactgatat taaatacaat aggcaatcca aaaataatag
    74881 gttcgcttat attgaatata gcgggagtta ccgataattt agcaaagtcc ttataatctt
    74941 ttctacgact gaagataaat attgcaatca ataaagcaat cgtatttccc gatccaccca
    75001 ttaccgcaaa ggtatcttta aaggccatat tgataatatg tggaatttgc tctttgtttg
    75061 cataagctgc catattttct tgcatattct gaatcaaaaa cggatctaac aatgaacctg
    75121 atattactgc ttgatggata ccaaatccaa aaaatagatt agcaatagat gtaattaaca
    75181 aaaatcctgg taaaccagtt gtaattttac ttaatggctc tgtaactatc gtggtaatta
    75241 gcttatttat atccatatta aacaattgaa tgattataaa tgacaataaa gcaaacgagg
    75301 taatcgtaat aatgattggg attaatacat taaaagactt taaaacagcg ggaggaatat
    75361 ttccggatag ctgaatctgt agtttcttat tattagaaag tttaataaat aattctgtag
    75421 ccgctaatcc aacaaatata cctacaaaca ttcctgaagc tcctgtatag gatataggta
    75481 tcacacccgt cacttcaaca gctttatccg cattttcagg agtaaacatc atctgcatag
    75541 gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc tgcaatatga tttgtataat
    75601 ttctgcttgc acacatatga taagatactg ctgctacaac taataatgta gttatgctta
    75661 atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt ggtcaatgta ctagtatcga
    75721 caatttcccc catgaatcct gttggttcta agatcactga attaataagt gtcacaaatc
    75781 ctgctaacat cataaatggc ataaatgtag caaacgcatc tcgcaaagtt cttaaatgga
    75841 tctgattccc caacttagtc gcaactatac caaatttttc aaatacaatg tccttcctgc
    75901 tacttttgcc catatttttt ctcctttatg cttattgtaa tcgattactt tttccaccca
    75961 aataatacat cacttatcaa aaaaagtaaa tacttttaat taaatatata tatctttttt
    76021 agtataaaaa aataagctct gaaaaaataa ttttcccgag cttatttttc aacaaaaaca
    76081 gcttcaaaaa caaagtcttg atataaatgt ctcgataccg aatactcgaa aggtttacca
    76141 gacgatagag caacaacttt gtctatctca atcataaatt cacctggatc tatttttaat
    76201 atttttgcgt caaattcatt cgcagtatct cctttgatac gtatgtgcga agtctttatt
    76261 tccagaccca gttcatttgt gatataagca tatacagatt tctttaaatg ttttggttcc
    76321 aagcctggta tgactgctaa tggcatgaat gtttgctcaa tcgagtatgg ttgacaatca
    76381 ataaaacggc ttcgtacaat attatataca tagtctttta cactgatact taatttatca
    76441 gctacttttt tcgatggaag ttcaattgag aaagtatgga tttctgaatc tacgtgttct
    76501 tcaccaacca cgctaaacaa accttcgttt ggaccaagaa ctcgtgagtt atcaattggt
    76561 gttctgacga cttcagtccc atacccactt ctacttcgca aaactccttc ggaaacaagt
    76621 aagtctacgc ctttctttac tgttaacttg cttgtatgat aaataactgc caattcatct
    76681 cctgtaggaa gtttctcacc aattttatac tctccattta caattttttt ctttatatct
    76741 aagtaaattt ccatatactt cgcaattttt ttcaatggtg accctccaat caaaaacata
    76801 tcttcatcta tactttaaca taaataaacc gataaaatcc acatcaaaat tatacacaca
    76861 ataaaaaaga tatatatatt taaacaaaac tatttacttt aagttgtaag cggtgttatc
    76921 attaagtgga aacaggaggt aaaaaaatta tgacaactat gaaaaagcta acttttccag
    76981 atgatttttg gtggggttcc gcttggtcag ctgagcaagc agaaggaaaa ggagaaacag
    77041 gaaaagcaga gactatctgg gatcgctggt ttaaagaaca accttaccgg ttttatcaac
    77101 gaatcgattc ctctattaca accgatcaca tccatcaata taaagaagat atagaactaa
    77161 tgaaaaaaac tgggcataac tcctttcgcg tttctatttc ttgggcacga atgtttccaa
    77221 atggcggtac cggaaaagta aatcccaaag ccatttcttt ctatcgaaaa ttattttccg
    77281 aaatgaataa taacggaatc aaagtatttg ccaacttata tcattttgac atgcctgcta
    77341 ctcttcaaga taaaggagga tgggaatcac gtgaagtagt tgatgcttat gttcattacg
    77401 ctgaaacttg ttttaaagaa tttggtgact tagtttgtca ttggtttacc ttcaatgaac
    77461 cacttgggcc tattttaggt acttatcttg aagattttca ttatccaaac ctcattgact
    77521 ttaaacgcgg agcgcaagca gcattcttca ctatacttgc acacgcaaaa gcaatacaag
    77581 tatttaaaga actagctctt cctagtaaaa taggcgttat tctaaatttg agtcctactt
    77641 ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga aatcgctgat ttattttata
    77701 cgcggagctt tttagatcct atggttaaag ggaatttttc taaaaagctt atcctgttat
    77761 tacaagaata taatcagctt cctgaagatt atacgaaaga agatcttgta tgtatctacg
    77821 aaaataccgc tcaaattctt ggactaaact attacgagcc acgtcgcgtc aaatctcgtt
    77881 cttctatggt ggatacatct agtccatttt taccagagtg gtttttcgaa ccacatatca
    77941 tgcctggacg acgaatgaac gaataccggg gatgggaaat ttacgaaaaa ggaatttatg
    78001 atttgtgcat ggatatcaag aaccattatg gaaacatcga agcatttatc tcggagaacg
    78061 gaatgggcgt tgcggatgaa gaacgtttca tggatgatac agggcaaata attgatgatt
    78121 atcggattga gtatatcaaa gatcatcttg cttacttatg gaaggcaata aatgaaggat
    78181 gtaacattaa ggggtatcat ctttggacat ttattgattg ctggtcatgg atcaacgcct
    78241 ataaaaaccg atatgggctg gtttcactcg accttactac tcaaaaaaga acaattaaaa
    78301 aaagtggcga attttacaag caattaagtg atacaaatgg atttagatac gatgtagact
    78361 tgttagttga ataatattct aaaattccac ttgaagtgaa cacattagaa aacaagaata
    78421 atgagaaata ccaataacaa atactttttc ttcatacttc atttatactt gtcagtataa
    78481 gagactgtga caaatgtagt gatcctcaga agttaggtct ggaaatctaa cttgagaaga
    78541 tttttttaaa ctacgtccag tcttttttat ttgtttaaaa ttgaagttga caagtcttca
    78601 ttaaaaattt agaatattca caagtttgac gacaattatg aaaatttatg tcgtgctaaa
    78661 ggagttttgt tattggatga aaagttagat ataaaaactg cgatcaaaga cacgttacct
    78721 accgtttttg gttatatcgg tattggactt gcatttggta tcgttgggaa agctgccggg
    78781 tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct atgctggttc tgcccaattt
    78841 atcacggtca gcatgcttgc tagccatagt ccactgctct ccatcgttct ctcgaccttt
    78901 ctagtcaatt cccgaatgat tctgatgagt atgacgattg ctccttattt caagaaaaat
    78961 cggctgcttc aaaatctgct gattggcaca ttgctgacag atgaaagttt cgcactggga
    79021 atgaataaat tgaactatac tggacaaaag ttaaactttc gttggatgaa tacagctaat
    79081 tggatttctt atttgacgtg ggtgggttct tctcttgttg gcgcactttt agggaatttt
    79141 attactgatc cgaaaaagtt tggtttggat ttcgccatcg tcgctatgtt tatcgggtta
    79201 ctttatcttc aagtcatttc tgatagaaac acctctaaac gattacagtt gatcctgatt
    79261 ggtctcactt tgattctggt atatgtcgga ctgattttta tcccaagtaa cttagtgatt
    79321 ttagttgtaa cactgattgg gtgcggattg ggggtgtgga tcaaacatgc cttcttttga
    79381 gtttacattg ctgactattt taggttgtac tttagcaact tggctttcta ggatcctacc
    79441 atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg cttgagtatt taagttttgt
    79501 cccgatcgtc attatgtctg ctttatggtt cagcagtcta tttacccaaa atatcggaca
    79561 tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt cctacacttt tagctgctat
    79621 tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt ctttcccttt cgttgattcg
    79681 cttattataa ataaaaaaac aagagcttat ggcaaattac tgtcatgcgc tcttgttttt
    79741 atcaaagtca aataagaaaa aaatcggcgt attcttgctt atccgttgaa cggaccacta
    79801 acaacgactt ttcctaatat atgtcctgtt tctacgtctt tggttgcttg tttcaagttc
    79861 ttcgcattca tcccatctga atagaccttg ctcaatgtac tgcggataat cccttcattt
    79921 gctaaagcag caatgtgcgc taaagcttct ccttgcgttt cgatttcata gttatagtct
    79981 gttttagcga acatatactc ccagtcgaag ctgatagatt ggtttttcca aacacctata
    80041 tctaacggct ctttgatccc tacgatcgtt cccacatgtc caaatggtcg gacaaggtgt
    80101 ttgatttgat caaagtagcg ggtaatatcg aataatacag cgacatactc tacttgatcg
    80161 attcctaatt cacgaaaact tttacttaag tcgtgatgat aatcgattgg atagtctaca
    80221 cctgtttttt tcagccattc aaaattcttc ggacttgcag tcgcgtagac ttctaggcca
    80281 gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc cagctccgtt gatcactaag
    80341 atttttttcc cgcggttggc attttcttct ggagtcagac caaatttttc aaataacaat
    80401 tcataagcgg tcaatgaggt caacggcaag gctgcggctt cttcatctga aagatttttt
    80461 ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt cttgattgct tcctgctctt
    80521 gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa atttttgcac ttcatcacct
    80581 acagcaacaa ctttcccgac tccatcaaac cctaagatac gtaaagcatt tcttatcccg
    80641 ttatcttggc gaagtttcgt atcgacagga ttaacagata ctgcgttgac ttttaccaga
    80701 agatcccgtg aactgggtac aggtatctgt tctttttcat caataaagct ttttggatca
    80761 tcgatcgaca acccttcata aaaaccgact accttcatta attccttttc tgacatacga
    80821 attcctcctt agcttcatca agttggagca tccatacagt tttcatttca atatatatag
    80881 tatgctattt gttccacttt tattcatcat aattattctc ttacttaaag taaactattt
    80941 gcactttttt ggttaaaaaa gaggttgcga caagactttt gtcacaacct cttttaaacg
    81001 gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg acaaaatatg aggagctatt
    81061 tttgtttggc tgttcttatt acttgatgca ggacagcttt ttcacgacct ctaattcacg
    81121 gtgttcaagc aagaaatcat tgttcactga aaagcgttgg atgttctttt tcaaaggtgt
    81181 ctaagatctc tcgtacttca tcagtagatt tcgtattcat caactgaact cgcaagtcat
    81241 ttgctcctgg aaatcctttg atatagattt taaagaaacg atgtagtcct gtgatggaac
    81301 gcgggatgat ttctgaataa tgatcttgca gatctagttg cagacgaagt agtccgatca
    81361 gttctttttc tgtatgttcc ctcggttctt tttcaaatgc ataaggattc ttgaaaatcc
    81421 cccggccaat catgatgcca tctacgccat atttttcggc caattcttct cctgtctggc
    81481 gatctgggat atctccgttg atcgtgatca gtgtttgcgg agcgatttgg tcacgtaaag
    81541 caatgatttt agggatgact tcccaatgtg catctacttt actcatctct ttacgcgtac
    81601 gcaaatggac agaaagattg gctatatctt gatgaagtag gtgactgatc cattcttcca
    81661 tctcagacat ttccgtatag ccgatacgcg tcttgacgct gacaggcaaa ccgccagctt
    81721 tagccgcttc aatcaattgt gccgctactt ctgggcgaag gatcaagcca ctgccttttc
    81781 cacgttctgc tacattaggg actgggcagc ccatgttgat atcgattcct tggaagccca
    81841 tttctgccat agcaatgctc atttctctaa aaaattctgg cttgtctccc caaatatggg
    81901 caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc tcgtacactg tcttttccat
    81961 ctggatgaca gaaactgtct gagtttgtaa actcagtaaa gaagacatcc ggtgctgctg
    82021 catgctttac gacatggcga aatacaacat ctgttacatc ttccattggt gctaaaacaa
    82081 aaaaaggctt tggcagctct ttccaaaaat tagtagtcat ttatttgtta ctctccattc
    82141 tatcaagcag tgaagtcgca atctttctca aaaacattgt cattatacta ggaaacaaaa
    82201 aaagaagcaa agtgaacagc ttaaaagatg tttctgattg agcaaaaaaa gactgtgaca
    82261 aaaatcttat cacaattcct tttccggaat aaatggtgtt caaaagcttg catctgcggt
    82321 aataagtcca accaaacgaa aaataggagg agctattttt gtttggctgt tcttattact
    82381 tgatgcagaa cagctttttc acaacctctc cactaaacgg tgttcaatca gctgaccctc
    82441 ggtattagct caacaaacga caaaatatga ggaactattt tcccgtttgt ttctctaata
    82501 ctcaggtcaa aacgctgatt tcacaacctc ttttctaact tctcaatcct cttcctttat
    82561 tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa tagcacaagt acgatcatcg
    82621 atgttgctac tggcacatcg attgttccaa ggaatccgta acggaaaccg gagatcatat
    82681 aaacgatcgg attgactttc gaaacagctt gccaaaatgg cggcaacata gagatggcat
    82741 agaaaacgcc acctagataa gttaacggct gcaagacaaa tgtaggcacg atcgatacat
    82801 cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc taacgaaaac aagattgccg
    82861 tcatcaataa tgtaatgatg accatgaacc atgaatacac atgaagtggt acaaagaaca
    82921 aggaaatgat cgttacgaga gagccgacga gaatactgcg acccactccg ccaatcacaa
    82981 agccccaaat gatcacatgc gtaggaacag gcgctaccaa gatttcttcg atgtttttct
    83041 ggaatttttg agagaagaag gacgaagaaa cgttggcata cgaactagtg atagcagaca
    83101 tcataatcaa accaggcacg atgaattcca tatatgaaaa accacccatg tcaccgatcc
    83161 gaccaccgat catttttccg aaaataacaa agtacaagga tgtcgtgatc acaggcggaa
    83221 ccagtgtttg gacccagata cgcaaatagc ggttcgtttc cttgatagct aaacttttta
    83281 atgcagtaaa ataaagatta aacatttttc tcctccgtat gattattttc ttctgtgatt
    83341 ttcaaaaaca gttcttctaa acgattcgac ttattcctca tagaaagaac tttgattcct
    83401 tgtcttgtca gctgatcgaa gattccgttc accccttgat ttcgttcaac ttcgacagcg
    83461 attgtctgtt catcttcaaa tgtacaagcg tatccttcaa tcacaggttt ggtctcatat
    83521 ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg atagaagctc tttcatacta
    83581 gtattctcga tcagttcacc tgattggatg atcccgatgt tgcgacaaag catttcagct
    83641 tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat tttcgttcaa ttctcggaga
    83701 aactcccaca tctcgcgtct caattcaata tcgactcctg ctgttggttc atccaagatc
    83761 agcaaacgtg gttcatgcat caatgcccta gcaatcatca gtcttcgctt cattcccccg
    83821 gaaagcatac gtgcacggac atttctcttt tcccaaagat tggactgctt caaatatttt
    83881 tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt agcctgcttg attcacgacg
    83941 atttgctgga ccgtttcaaa gggattgaaa ttgaactctt gcgggactaa tccaatctgt
    84001 tgctttgctc ggactagatc cgtatccaga tcataaccga agacattcac ttttccagat
    84061 gttttattga ctaaagaagt aatgattccg atcgttgtag acttcccagc tccatttggt
    84121 cctaatagcg catagaaatc tccttctttc acggaaagat cgattcctcg caatgcttcg
    84181 accccacttc cataaacctt tttcaaatta tttatttcaa gcgcatttgc catttatttc
    84241 ctatctcctt atagacatga ttctttattg tatcatggaa tacagcagat gaaaaaacaa
    84301 cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa atctcctaat gaaagacatc
    84361 aatcggacga agtcagacaa ccttttcaga aaaaacaaat cgaagataaa ggattactat
    84421 tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga acacttttgt ctcacgctct
    84481 aaacacgtat aaacggcgga agcaaaagcc aagatttttg atccaataaa tactttcgat
    84541 cacttcgcct atcccaatac atgccactgc cgcttttcat caaataacgc ggcttccgtt
    84601 tttgcggttt ctactaaagt ctctgggtaa tccaaaactt tcagcaaggc aatcgcattt
    84661 cgggtccttg aaggtccttc ttttaatttg aaatcaaaac tgattccttt tccttctgtt
    84721 acttgttctt caaagtggac attctcacaa taattcttta aaatctctgt caattcgata
    84781 tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa gccaccgaac gactgaagcg
    84841 gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa tctcatctac gaagcagtag
    84901 cagcgttctt tggaagccac ttggtccaac aaacgtttga tcgatttgat ttctgaaaca
    84961 aaataactgt cgccttcaaa taaatcatct tcgattgcca tggaagtcaa cacatgtcca
    85021 ggttgcaggg taaactgttc tgcaatcgct gtttggatcg tctgtgctag aatacagctg
    85081 atggctatac ttttgacata cgtagatttt cctgatgcat tggaacccgt gactaatgta
    85141 ttcttctgcc agttgaccgg attcactacc gcttctttta acaatggatg atagatatct
    85201 ttcgctgtca caccgccctc agtaaactct ggtcggcaag tgatgggcat ataagtacgg
    85261 taattcaata ttgcagcggc tacttctaat tctcccagca attcccacag cttgatggct
    85321 tcagttgaat gcttttctaa tttagtgaga acgaaatgat aggagataaa tggcagcatg
    85381 aacatgatgt tgacatattc aaaaatcaac tctgcttcac tcccactctt catacgaaat
    85441 gaaaaaccta atttagggat gtctcttaat ggtttagttt gctgtttcag ctcttcttga
    85501 agtggtgtcg gcagttttgc tagctgattg gcggctgaaa tcgttgaaac caaataactc
    85561 atgctattca gctctgtttc tagtgtttgt ttcttcacta gataatagac cgtattgaat
    85621 actaacccac cgattgccaa atagattcct agtacttgtc ctaaaagaag caaacccaaa
    85681 cccaccagcg gaagcaagcc aagaaaaata aataaaccta cgcgtccaat ctttttactt
    85741 tttccatccg ctagatattg ttttgcaaaa ttatgatctt tttttcctaa acgagcgaat
    85801 tgatattgga ttttttctct cagttgagga ttttcctgat aaaaagcaat cagtttttct
    85861 aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt gatacaacgc ttctgaacct
    85921 acactagaat aggtactgtt gattccttcg aatacttcga acatatccag gtcataccaa
    85981 gtaagatcat cgatttcgct atcccatgaa cggaacttct tctccgtgag ccatgcttcc
    86041 ttcaagcttt cttctttatc aaatctcggt tgataaggaa ttttttccca tttgctacga
    86101 actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag caactaataa aataacagct
    86161 gcaataataa ttaaaacgat aaattctctt tgacccatat ccctctccgt tcctttattt
    86221 gttagcttta gtatagcact gttgcgctat ttgaaaagag ggtaaaaaaa cacctttcaa
    86281 aataaaaggt gtttttttct tgtatattac atatttatct ctcatttatc aaaattgaca
    86341 aataatcata gggaggaatt gttgttaatt cttgtttatt ctccagtgca ttgacaactg
    86401 tcccaaggga aaggatagcc tcaggtacta ttttttcttg ttctgttgga ttagctaagc
    86461 gaagaacaaa tccttgttca tgaaaataag aaggataaat cgatgataca attaagtctt
    86521 caggtaattc aatcaatgaa aatttacgag gaacatcaag ttcattttgt aatggccaaa
    86581 tcttgttatc taacctgtga ataaatacat tcagtttctg cttttgataa gaaatagatt
    86641 gttctaaccg tttttctgcc tgttttgcca ctttgaactc attaaatctt ccttcttcta
    86701 cattaaatcc aaaagtgact ttatactcac ctatctcttg agctagcggt gtttccatca
    86761 tgacatgccc ttcatttgtc gtatctcctg aagctcgccc aggtcgccaa gcaagattag
    86821 gttttccaag ttcccctgtt gttgaaaata aggttatgat taattgattt tctatacgtt
    86881 catactcttt taaaccatct gcataaaacg taaaataatg atcattttct gccacagaga
    86941 cactcttatc gaatatttct aaattaacag gtttctctac catagtttct gaccaattcg
    87001 gaggaatgac atcaggggta ttttcaataa atccgttttg aatttgtgca attgattttt
    87061 cagatttatc taaaacatta attgctaagc gcatacgatg cgagtaaaca gtattatgaa
    87121 ttttgagtgt gccttcaacg atttgacttt ctcccagact gaacgatatt tcataagata
    87181 tttcttccat ttcgggtttt tcagataaac gatcttttaa atctttagga agttgagctt
    87241 ttccgtaaac aactaatgtt tcttgtaaag aatctttata tactttagct gtttctaatt
    87301 ttagagatag ttctgtatct ccttctaatg gtgaataatc atacgtatcg ccatcatttg
    87361 ctgaatcgat caaatgaacg aaatcaagat attgtctatc tccaactatc aaattcaatt
    87421 gcccatcttt aaaagaaagt ttatatttat tgttacttat ctcagttttg tctatctcga
    87481 ttgctgtttc taatttttca ttatttttct caaattcaac aacagtgtat cctagtgctg
    87541 gcacatttac atttattttt atgtcaagtt cataataagc tggttcatca aaatattcga
    87601 aacctgtagc aatcatccga taagcatttt tccgttctgg aaaataaact tctttttcaa
    87661 tgatattata gggactacct ttgaagtgta ttttctttga atcagctact acatgcacga
    87721 ttttcgatcc actataattc tcagtcgttg tgttgaaaac aaggacttca ttcgatttta
    87781 gctttaatgc atcagccatc cgttttacaa taaggttttc gattccgtca gctatctcat
    87841 tagcttcttt gattcgatgg aaaatatctt ccgcaacatt atctgaaaca cagccaccaa
    87901 tactatcatg agcctggttt tgcaaaatct ttttccataa ccgacgaagt aggcctttac
    87961 tgatatggat accatttctt tctgcaatca gcatgagcgg ctcaactcgt tttagaattt
    88021 tttgttccaa tttaaaattt tctattttca tttttgttct tactgagcca atagaacgat
    88081 gaacacgaga gtaaaccggc tgacgaagtt cacctttata ttcaggtaat tcttttttgt
    88141 ttctgataac ttctacataa gaaggataat ctgaaataat attgtggaat ttactgtatt
    88201 tattcaactt ctctacgact tgatcaaaat ttaaaatcat tgacttttga tctacacccg
    88261 aaggtactaa aatatctttg ttttttcctt gcatatgaag catttcaact gcttcatcca
    88321 acttttcttt tgcaaattca ggaatatctt taattgcgtc taaggtcatc attccagtca
    88381 tataaccaaa tggaaaattc atggcataaa caaatttatc tcccaatcct ttccagataa
    88441 aataaggaga tggaaccatc gtttcaaagt ttaatcctcg ccaacacatg aaattatcga
    88501 tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa cccaaacgtg tcaggcaaat
    88561 accctaccat cattggatct ccatatttaa ggatcgtatc actgattcca atcattaagt
    88621 ttcttaaaat tgattcagca tccactaata aggcatctgt ttgtgcatac cacggtccca
    88681 cgaatagttg tcctgttttc atcagtcgtt ggataacctt aagcttttgc ggattgatct
    88741 ccaaatactc atcgacgatt gaagtttgtc catctaaaca aaagtttact ttagggttcc
    88801 tttccaattc atctagaact tcagtaaaga cctgatcaga caatacgatg gcatcttcat
    88861 ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat tatatgtgca tttaccatat
    88921 cagattctcc tactttcctt cttctactga atttacttgt tgttcttttt tatacttgat
    88981 ggattttagt gcattgacca ttaacgctgt aaccactccc ccaataacaa ttgatactaa
    89041 atataaaggt atattcgtaa caaaaggaat tgccataaag gttgaaagca ttgtttcttg
    89101 agtaacacca aaaaatccat ttagaccaca tgctactgca gtgcccacca cgatgcttgg
    89161 tattactcgt aaaggatctt tcacggcaaa cgggatcgtc ccttcagtaa tccccacaag
    89221 gcccataatc atagctgtct ttgcattatc tcgttccgat tcatcataaa gttttttgtt
    89281 taaaattgtt gctaaaaaca ttccaaatgg cggaatagca attcctaata acatggctgt
    89341 actaggtcca aagcttccgc ttgaaaaaga agcaacccca aaagcatatg cgattttatt
    89401 gataggtcct cccatatcaa acgccatcat tcctcctaaa atagccgaca tcaaagcagc
    89461 atttgctcct gacatgcctt ctagccaatt agtcagtcct gttgtcattg cttgaatagg
    89521 cgtagctagt acataccata atatgattcc aacaactaat gtagtaacaa aaggaatcag
    89581 cataattgat tttagactaa taacttgcat cggtactttt acttttttca atgcattagc
    89641 taaatagcca caagctaatc cagcaaccat tcctccaata aatcccatac caagattttg
    89701 acacatcatc ccagtgataa aagctggtgc tagaccggga cggtcagcaa ttgaatatgc
    89761 gatataggct gaaatcatcg gtacgataaa ctgtaatccc gtctgaccaa tcattcgcag
    89821 gactccccag aatgtatccg tctggtccca aacacctgtg ccaccaaaaa tgactgcaat
    89881 agaagttagt aaccctgctg ctacgacaag cgggatcata taggaaaccc cactttggaa
    89941 atgatttaga atttctcttc cttgtttctt gaattccatt agtaactccc ccacgatata
    90001 ttttttattt gttaattaat gcaattgctt catcaatcgt ctcttcacca tcttttacta
    90061 cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa gcgacctcgt tctgaaacat
    90121 ttgtatctac tgcaaatatg acaacatctg cctcggaaac atccgaaatt gaaagtttgt
    90181 tttctatccc catagccccc tgtgtttcta ctttaatatc taaccctttc ttcttcgcaa
    90241 acttttctaa attagctttt gccatataag tatgggcaat ccctgcgata catgctgtta
    90301 ctgctactat ttttgtcata tgaaattcct ccttttaaaa tgatagagct tttaccaatt
    90361 cttttttatt tctcgaatct tttattcttt taacaaactc gtcatccatc aatcttccag
    90421 ataattctgc taaaatacgt aaatgattag tacctttatc agtgtctttt attgctaaca
    90481 aaaagacgag atcaactggt tgatcatcta atgcgcccca ttcaacagac cgctttaact
    90541 tggctacagc cactgttgat tcaattactg ctaggctttt gccatgagga atggcgatat
    90601 ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa tacgtctttt tgaaattgtt
    90661 tgtggtcaga cacaaaccca ttagtctcta acaatttcga taatttttcg actacacttt
    90721 ttttatcaga tgcatttaaa tctatcacta tattatttat attgattttt tcactgatca
    90781 tttataagcc ttctttcaat atataaacta atttttcaaa cgacgaactc gtttttaaat
    90841 ttgctaataa ctcatgattc gaagcgattt cattgaatgt ttcataaatt ttattcaatt
    90901 caggttttac tttttcatta agtgcaaaaa agaaaacaga atgaacaagt gtcccatccc
    90961 aatcaattcc ttctggatta acgtagacac agattactgg cttttttata aaatctactt
    91021 cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga tagtttttct cttttgattg
    91081 ctccctctag tatcccttct ttcgcgtaat tttcttttac caattttttg gtaattatat
    91141 ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa aaagcaaaat cgtttatcta
    91201 ataaatttag aaaagcatct tctgtttgtt catttaactg tagatactgt tttacttttc
    91261 tgacatctgc ccctaacatc aaaggactaa caacaataac aggaaaattt gtattttcaa
    91321 tcggaatcgt gcttataaca agttcatcag tgacttttct atcttgtagt tcattgatgc
    91381 ttagtacatc ttctatctca accatatctg caaaaccgtt atttattctt tgctctaata
    91441 attttgatgt tccatatccg ctactacaaa ctaatatcac tttcgttttg tcaggtttgt
    91501 aacgatccaa aagtgattga atatggagtg ttacgtaagc gatttcatct tcattaaaat
    91561 gtatacagaa tctctcttcg atttcctcta ataaatctac acttatcata aatgaccttt
    91621 taaaagaata tttgattttg tccgtataag ggttatagat atttactccc aatttgagtc
    91681 ttttaactgc tgcattgaga tgtaatacta gattattgat caattcttca tcaggataaa
    91741 attcatttaa tttaattctt actaactctt gtagctcaac acccatatct aaataggaaa
    91801 gcttcgtttg atctaagtcg tgattcatag catttttttc aatcgaagta atgtgaatgt
    91861 caacataagc ttgttcatct attggcacaa taattgaaaa tgctttttca atcattttga
    91921 ttaaaataag tgtattcttt gagatttctt cttcagaatt attactacgt gataagaaat
    91981 tgcttgtttc aatcctttct atcgcaatag caaggtggat agctaaagtt tgaaattcat
    92041 aatctgtaaa tttcaattgt gtcttattca tgtatcgatt aagcaattcg gctacttttt
    92101 tcaatatgtc cttatttaag aaattattcg cttcaccaat gaaattgata ttcaagtcta
    92161 agagatctgt tttactgtta gatgctgtaa agccacccca ataatagttc acaacttctg
    92221 acatcaacct tcttttactt ttttcatcag ctttgatgcg cacgccattt cgatctgaaa
    92281 ctaaggacat cccatgcttg tttagtcttt ttctaacatc tttcattgtg ctttctaccg
    92341 tcgacctgct cacgtataat tcgtcagcaa gattttgaat ctttagagaa tcattagtat
    92401 tcaataattt agcaaacaca taagttagac ggtcctccgg agagtctgcg atattgattt
    92461 tatgactgga taataaattt cgaagtgttt ctttttttcc aataagcgaa acgccttttt
    92521 taggttgaac caacatttgt acatctggca ttttttggat tatttctttc aaaattaaaa
    92581 tatctttgct aattgttttc gtagatacat ataattgttc tgataattct ttgtaagttg
    92641 tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg ccgttcgcta aattcgatgc
    92701 ccattatctt cacctcctga atacagctta agtgtacaat tattatctct attttcatac
    92761 agaagaaagt tcttgattaa tgtagaagtt tctaatattc ttgattattt caaatcaata
    92821 aaaaa
//
LOCUS       DANJMI010000004        92020 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000004 DANJMI010000000
VERSION     DANJMI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 92020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 92020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.004"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(551..2164)
                     /locus_tag="PWU81_000333"
     CDS             complement(551..2164)
                     /locus_tag="PWU81_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL1109070.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2305..3213)
                     /locus_tag="PWU81_000334"
     CDS             complement(2305..3213)
                     /locus_tag="PWU81_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1109071.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3396..4520
                     /locus_tag="PWU81_000335"
     CDS             3396..4520
                     /locus_tag="PWU81_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL1109072.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4747..5664
                     /locus_tag="PWU81_000336"
     CDS             4747..5664
                     /locus_tag="PWU81_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1109073.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5657..6490
                     /locus_tag="PWU81_000337"
     CDS             5657..6490
                     /locus_tag="PWU81_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1109074.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6573..7691
                     /locus_tag="PWU81_000338"
     CDS             6573..7691
                     /locus_tag="PWU81_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL1109075.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7685..9583
                     /locus_tag="PWU81_000339"
     CDS             7685..9583
                     /locus_tag="PWU81_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HDL1109076.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9577..10800
                     /locus_tag="PWU81_000340"
     CDS             9577..10800
                     /locus_tag="PWU81_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL1109077.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10886..12820)
                     /locus_tag="PWU81_000341"
     CDS             complement(10886..12820)
                     /locus_tag="PWU81_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL1109078.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13046..13702
                     /locus_tag="PWU81_000342"
     CDS             13046..13702
                     /locus_tag="PWU81_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL1109079.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13776..14129)
                     /locus_tag="PWU81_000343"
     CDS             complement(13776..14129)
                     /locus_tag="PWU81_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL1109080.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14321..15250
                     /locus_tag="PWU81_000344"
     CDS             14321..15250
                     /locus_tag="PWU81_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDL1109081.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15261..16097
                     /locus_tag="PWU81_000345"
     CDS             15261..16097
                     /locus_tag="PWU81_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HDL1109082.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16345..17121
                     /locus_tag="PWU81_000346"
     CDS             16345..17121
                     /locus_tag="PWU81_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1109083.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17297..18040
                     /locus_tag="PWU81_000347"
     CDS             17297..18040
                     /locus_tag="PWU81_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1109084.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18033..19613
                     /locus_tag="PWU81_000348"
     CDS             18033..19613
                     /locus_tag="PWU81_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDL1109085.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19864..20343)
                     /locus_tag="PWU81_000349"
     CDS             complement(19864..20343)
                     /locus_tag="PWU81_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL1109086.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20359..21111)
                     /locus_tag="PWU81_000350"
     CDS             complement(20359..21111)
                     /locus_tag="PWU81_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL1109087.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21228..21298
                     /locus_tag="PWU81_000351"
     tRNA            21228..21298
                     /locus_tag="PWU81_000351"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21260..21262,aa:Gly,seq:ccc)
     gene            21659..22384
                     /locus_tag="PWU81_000352"
     CDS             21659..22384
                     /locus_tag="PWU81_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL1109088.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22639..22905
                     /locus_tag="PWU81_000353"
     CDS             22639..22905
                     /locus_tag="PWU81_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDL1109089.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22902..23333
                     /gene="ruvX"
                     /locus_tag="PWU81_000354"
     CDS             22902..23333
                     /gene="ruvX"
                     /locus_tag="PWU81_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL1109090.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23358..23663
                     /locus_tag="PWU81_000355"
     CDS             23358..23663
                     /locus_tag="PWU81_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDL1109091.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(23744..24889)
                     /locus_tag="PWU81_000356"
     CDS             complement(23744..24889)
                     /locus_tag="PWU81_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1109092.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(24950..25597)
                     /locus_tag="PWU81_000357"
     CDS             complement(24950..25597)
                     /locus_tag="PWU81_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1109093.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            25791..26084
                     /locus_tag="PWU81_000358"
     CDS             25791..26084
                     /locus_tag="PWU81_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109094.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            26128..26439
                     /locus_tag="PWU81_000359"
     CDS             26128..26439
                     /locus_tag="PWU81_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109095.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            26436..27060
                     /locus_tag="PWU81_000360"
                     /pseudo
     CDS             26436..27060
                     /locus_tag="PWU81_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            27201..27359
                     /locus_tag="PWU81_000361"
     CDS             27201..27359
                     /locus_tag="PWU81_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109096.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            27360..27722
                     /locus_tag="PWU81_000362"
     CDS             27360..27722
                     /locus_tag="PWU81_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109097.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            27759..28607
                     /locus_tag="PWU81_000363"
     CDS             27759..28607
                     /locus_tag="PWU81_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HDL1109098.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            28597..30072
                     /locus_tag="PWU81_000364"
     CDS             28597..30072
                     /locus_tag="PWU81_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDL1109099.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            30338..30745
                     /locus_tag="PWU81_000365"
     CDS             30338..30745
                     /locus_tag="PWU81_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HDL1109100.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            30748..30963
                     /locus_tag="PWU81_000366"
     CDS             30748..30963
                     /locus_tag="PWU81_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109101.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            30967..31347
                     /locus_tag="PWU81_000367"
     CDS             30967..31347
                     /locus_tag="PWU81_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL1109102.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            31481..31636
                     /locus_tag="PWU81_000368"
     CDS             31481..31636
                     /locus_tag="PWU81_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HDL1109103.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            31705..32178
                     /locus_tag="PWU81_000369"
     CDS             31705..32178
                     /locus_tag="PWU81_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDL1109104.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            32175..33869
                     /locus_tag="PWU81_000370"
     CDS             32175..33869
                     /locus_tag="PWU81_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL1109105.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            33835..34020
                     /locus_tag="PWU81_000371"
     CDS             33835..34020
                     /locus_tag="PWU81_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109106.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            34024..35199
                     /locus_tag="PWU81_000372"
     CDS             34024..35199
                     /locus_tag="PWU81_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL1109107.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            35192..36715
                     /locus_tag="PWU81_000373"
     CDS             35192..36715
                     /locus_tag="PWU81_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL1109108.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            36771..37055
                     /locus_tag="PWU81_000374"
     CDS             36771..37055
                     /locus_tag="PWU81_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL1109109.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            37042..37377
                     /locus_tag="PWU81_000375"
     CDS             37042..37377
                     /locus_tag="PWU81_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL1109110.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            37672..38298
                     /locus_tag="PWU81_000376"
     CDS             37672..38298
                     /locus_tag="PWU81_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109111.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            39040..39876
                     /locus_tag="PWU81_000377"
     CDS             39040..39876
                     /locus_tag="PWU81_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL1109112.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            39940..40371
                     /locus_tag="PWU81_000378"
     CDS             39940..40371
                     /locus_tag="PWU81_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1109113.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(40433..41755)
                     /locus_tag="PWU81_000379"
     CDS             complement(40433..41755)
                     /locus_tag="PWU81_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HDL1109114.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(41909..43549)
                     /locus_tag="PWU81_000380"
     CDS             complement(41909..43549)
                     /locus_tag="PWU81_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDL1109115.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            43882..44352
                     /locus_tag="PWU81_000381"
     CDS             43882..44352
                     /locus_tag="PWU81_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HDL1109116.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            44574..45677
                     /locus_tag="PWU81_000382"
     CDS             44574..45677
                     /locus_tag="PWU81_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL1109117.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            45961..46641
                     /locus_tag="PWU81_000383"
     CDS             45961..46641
                     /locus_tag="PWU81_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL1109118.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            46731..47765
                     /locus_tag="PWU81_000384"
     CDS             46731..47765
                     /locus_tag="PWU81_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL1109119.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            47779..48114
                     /locus_tag="PWU81_000385"
     CDS             47779..48114
                     /locus_tag="PWU81_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109120.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            48098..50578
                     /locus_tag="PWU81_000386"
     CDS             48098..50578
                     /locus_tag="PWU81_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109121.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            50634..52193
                     /locus_tag="PWU81_000387"
     CDS             50634..52193
                     /locus_tag="PWU81_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1109122.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            52190..53554
                     /locus_tag="PWU81_000388"
     CDS             52190..53554
                     /locus_tag="PWU81_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL1109123.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            53725..54795
                     /locus_tag="PWU81_000389"
     CDS             53725..54795
                     /locus_tag="PWU81_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1109124.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            54810..55478
                     /locus_tag="PWU81_000390"
     CDS             54810..55478
                     /locus_tag="PWU81_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1109125.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            55498..56076
                     /locus_tag="PWU81_000391"
     CDS             55498..56076
                     /locus_tag="PWU81_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1109126.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            56477..57388
                     /locus_tag="PWU81_000392"
     CDS             56477..57388
                     /locus_tag="PWU81_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL1109127.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(57766..58683)
                     /locus_tag="PWU81_000393"
     CDS             complement(57766..58683)
                     /locus_tag="PWU81_000393"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDL1109128.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            58885..59322
                     /gene="zapA"
                     /locus_tag="PWU81_000394"
     CDS             58885..59322
                     /gene="zapA"
                     /locus_tag="PWU81_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL1109129.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            59380..59928
                     /locus_tag="PWU81_000395"
     CDS             59380..59928
                     /locus_tag="PWU81_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDL1109130.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            60043..62403
                     /locus_tag="PWU81_000396"
     CDS             60043..62403
                     /locus_tag="PWU81_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDL1109131.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            62490..62804
                     /gene="trxA"
                     /locus_tag="PWU81_000397"
     CDS             62490..62804
                     /gene="trxA"
                     /locus_tag="PWU81_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL1109132.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            62986..64764
                     /gene="uvrC"
                     /locus_tag="PWU81_000398"
     CDS             62986..64764
                     /gene="uvrC"
                     /locus_tag="PWU81_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL1109133.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            64909..65808
                     /locus_tag="PWU81_000399"
     CDS             64909..65808
                     /locus_tag="PWU81_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1109134.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            65801..67063
                     /locus_tag="PWU81_000400"
     CDS             65801..67063
                     /locus_tag="PWU81_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1109135.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            67224..69122
                     /locus_tag="PWU81_000401"
     CDS             67224..69122
                     /locus_tag="PWU81_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HDL1109136.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(69452..70171)
                     /locus_tag="PWU81_000402"
     CDS             complement(69452..70171)
                     /locus_tag="PWU81_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDL1109137.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            70508..70840
                     /locus_tag="PWU81_000403"
     CDS             70508..70840
                     /locus_tag="PWU81_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109138.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            70827..72830
                     /locus_tag="PWU81_000404"
     CDS             70827..72830
                     /locus_tag="PWU81_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDL1109139.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            72849..73319
                     /locus_tag="PWU81_000405"
     CDS             72849..73319
                     /locus_tag="PWU81_000405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDL1109140.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            73340..73924
                     /locus_tag="PWU81_000406"
     CDS             73340..73924
                     /locus_tag="PWU81_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HDL1109141.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            73939..74925
                     /locus_tag="PWU81_000407"
     CDS             73939..74925
                     /locus_tag="PWU81_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HDL1109142.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            74915..75226
                     /locus_tag="PWU81_000408"
     CDS             74915..75226
                     /locus_tag="PWU81_000408"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDL1109143.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            75248..77029
                     /locus_tag="PWU81_000409"
     CDS             75248..77029
                     /locus_tag="PWU81_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDL1109144.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            77022..78398
                     /locus_tag="PWU81_000410"
     CDS             77022..78398
                     /locus_tag="PWU81_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDL1109145.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            78405..79040
                     /locus_tag="PWU81_000411"
     CDS             78405..79040
                     /locus_tag="PWU81_000411"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDL1109146.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            79070..80425
                     /locus_tag="PWU81_000412"
     CDS             79070..80425
                     /locus_tag="PWU81_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDL1109147.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(80617..82011)
                     /locus_tag="PWU81_000413"
     CDS             complement(80617..82011)
                     /locus_tag="PWU81_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL1109148.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            82103..83074
                     /locus_tag="PWU81_000414"
     CDS             82103..83074
                     /locus_tag="PWU81_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDL1109149.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            83071..83853
                     /locus_tag="PWU81_000415"
     CDS             83071..83853
                     /locus_tag="PWU81_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL1109150.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(84022..84393)
                     /locus_tag="PWU81_000416"
     CDS             complement(84022..84393)
                     /locus_tag="PWU81_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1109151.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            84527..84970
                     /locus_tag="PWU81_000417"
     CDS             84527..84970
                     /locus_tag="PWU81_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL1109152.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(85013..85822)
                     /gene="yidA"
                     /locus_tag="PWU81_000418"
     CDS             complement(85013..85822)
                     /gene="yidA"
                     /locus_tag="PWU81_000418"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDL1109153.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(85841..87208)
                     /locus_tag="PWU81_000419"
     CDS             complement(85841..87208)
                     /locus_tag="PWU81_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL1109154.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(87192..88022)
                     /locus_tag="PWU81_000420"
     CDS             complement(87192..88022)
                     /locus_tag="PWU81_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDL1109155.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            88185..88622
                     /locus_tag="PWU81_000421"
     CDS             88185..88622
                     /locus_tag="PWU81_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDL1109156.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            88698..89318
                     /locus_tag="PWU81_000422"
     CDS             88698..89318
                     /locus_tag="PWU81_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDL1109157.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            89507..90013
                     /locus_tag="PWU81_000423"
     CDS             89507..90013
                     /locus_tag="PWU81_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDL1109158.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            90030..90929
                     /locus_tag="PWU81_000424"
     CDS             90030..90929
                     /locus_tag="PWU81_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL1109159.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            91016..91900
                     /locus_tag="PWU81_000425"
     CDS             91016..91900
                     /locus_tag="PWU81_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL1109160.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT        30402 a        15609 c        18940 g        27069 t
ORIGIN      
        1 taaataaata ggaacaaaaa aattaaatta tttaatacac acattaggga tcattcattg
       61 aaggattaga agacaacgag gatggcgaaa aatatgatta caatagaaaa gtcgttctaa
      121 accaatcagt tcctagtatc acacaacgca agccagtcga tttggtgaga ttagaggagt
      181 gaaagcaaac tttattatat aagaaaaaat attcgaaagg gatgctagtt taggtactag
      241 tgctgataga ttaatatgat gcctcgtaat attaggcgta gttactctag agacatcact
      301 atggactatg atttgtctca gagtcacaac atgttttttt atgaaaagaa acagctaaaa
      361 taattaaatt gcgcaatgtt taaactttct taaactataa cctaataaat gtgaagaaat
      421 aatgcacaat ttcttcacat ttatcttcta ttctatatac ataccaacca acaaccctat
      481 atactttttc atttttactc ctcaagtaaa aataactcct agaccgcggc tacccaccgc
      541 ggtcgttttt ttattcttca atcaaaatcg ctttgatacc caaacctgcc aagattcggg
      601 cagaagtata agtattcaat cctcttctga aagtaatgta aactttttta tcttttggga
      661 tttcattgat tcgatctcgt aactcattca ttggaatatt ttttgttgcc gagatactgc
      721 cagaaggtgc tttattaggt tcacgtatat ctaaaaagaa cgcttctttc aaatcgtttt
      781 cgggtatatc acttgctttg atcgtttcta cgatattcgt catttgattg attgcgacat
      841 aaccagctat attcaacggg tcacgggtag tagaatacgg tggagagtaa ggcaactcca
      901 aatcaggcaa atcaaagact gtcaagtttc cggtgattgc tacagaaagt tctcccatcc
      961 gtttatcgac acctttttct ccaaatgctt gtccacctaa aatacgaccg ctatctgcat
     1021 caaaaatcag ctttagattt aatcttgtag cttctggata gaaataggca tggtcaaagg
     1081 gagtgataaa aacagtttta taatttgtta tgcctgatgc ttttaattca tgttctgtca
     1141 ttcccgcata acttgctgta tgatcaaaga tttttgcgac acctgcacca atatatccgc
     1201 gatagcgcat aggcgtacca tttaaaatat ctgccagcat atgtccttga cgattggccg
     1261 cactggaaag catactttgg atcggctgac cagtcactac actagtcgtt tcgataatat
     1321 cgccaatcgc atagatgtcc ggcagattgg tttgtaactg gtcatcaaca atgatctgac
     1381 ctgtatctga taattgtatg ccggttgctt tcaccacttc attcttcgga gaaacaccaa
     1441 cagcaaaaat tagcatatca gcagaaagga cggagccatc tgataatact atttctcgac
     1501 ctttatcacg gatctcagta actcttgttt ctaaatgaac ggctaatcct tctttcagca
     1561 atttgtcata aactaaatta gcaatttctg gatcataagg gaaagcgact tgtggcaatt
     1621 gatcgattaa agtcacgtct aagccgcgat gcttgaaatt ctcagctaat tccagaccca
     1681 taacacctgc accaagaatg acgacatgtt gtggattttt ttcttcaagg aacgatttga
     1741 ttcgatccgc atccgtcaca ctacggagga caaagccgtt ttctgcttca aaaactccgg
     1801 gaatatccgg ataatctggt ctagcaccag aagagatgat caacttatcg taatctgttt
     1861 ttatttcttc atttgtggat aggtctttaa ctgttaatcg cttagtagaa ggatcgattg
     1921 ctgttacttc gtgtttagta aagacatcga tgttgttttt tgttttcaat atttctggtg
     1981 tacgttcgat cagcgagtca cggtcagtga tcacacctcc taaataataa ggcaaagcac
     2041 aacttgcata agaaatattt tctccgcgtt catagataat gatttcgtgc gcttcattca
     2101 atcgacggaa acgagtggca aatgaaggac cgccagctac accgccaatg acaacaactt
     2161 tcataaaaac atctcctttt ctacgttgat cgtactatgt tatggtaaaa aacagcaaat
     2221 aattgtgccg gaaaattatt gtttattttg aatagacaga aaatatagag aaataatttg
     2281 tggataaaac ctaaaaattt ttttctattt taaggataga taatactttg aaggggataa
     2341 ccctgtatat tttttaaaag ctgaagaaaa atgatggatc gaatgaaatc ctaatatatc
     2401 tgcaatttct gtataggtta attcattttc tagcacgaga cgttttgctt tttccatttt
     2461 tagttcttta aaatagctaa taacactttt tccattcact tttctaaaat gtttttttaa
     2521 gctggctgga ctgataaaaa aaatttgggc taattgttca agcaaaaaat tttctgaaag
     2581 atgttcttct aaatatcgta tgattggatc aactaattca tctgaataaa gaatatgctt
     2641 ttttttagga atgactttct catttgaagg tattgtcaat aaagcaagtt cctgttgata
     2701 atgccttggt aaggaagaca attgaatttt ttgcagacaa taagtaacta ataaggaaag
     2761 caaatgaatg ctcatctgtt ggcatagtat gaaaaaagaa ttgatttcct gatcatagga
     2821 aatagctggc ttactatgtt ttagataatc aattttgaac tgttcatttt tcagaaaagt
     2881 aatgatagat gttatttctt gatctataga aaaagattgt cccgtaatat gtgctaataa
     2941 ttgctcatca aaataaaact ggataatgat aaaagtttgt gtttcctttt gagtagaaaa
     3001 gatttggact tcttgctgtt tatcaatgaa taaaagggaa ggattatcta gttctataga
     3061 tttatgtgca aattcagcat ataaattagc gctttcattt attagtagtc gaaaataggg
     3121 agcagcagat aaatgaagat gggtatctat ggaataggtt agtgtttttt tgacaagatt
     3181 ggaggattgg ttgtttgtta acaaaagaac cactttcctt tctcttttta tataaaaagc
     3241 tttctattta taattaatga ctattataac aaaaatgaaa agccaaaacg acaagtaaaa
     3301 tattttttta tataaatatt tgtgttacta attccgttaa tatacataag tgaaagcgtt
     3361 ttatataaga acttgaaaag aaagaaggga tagacatgaa aaaaagatac aaattggtga
     3421 tagctttgct aaacattacc ctattattca gcttaagtgc atgtggaaaa actaacgaag
     3481 aagtaacagc aaacatcgca gatccaatga taaaaaaagc cactacatta gaagtatatg
     3541 tcgcaaacgg caaactgaca aactatgttg aaaatgctgc aaaaatctat aaccgcaaaa
     3601 ctggagcaaa tattgactta aaaattgtga atgtagcttc gggaacagca acagtacaaa
     3661 tgatcacccc aaaattggta acacacgaag atatgccgga tattatctcc atggcagaca
     3721 cttcagcagc aggtgtgctg gaaaaatttc aagatgcttt ttatagtgct gatgaatatg
     3781 gattttatga caaatatggc gatcagtttt atgatcaaaa attgaatatt ttgcgtcaac
     3841 aaacgagtaa tgaaacgatt attccttggg ctagtgattt taccccagca ctttgttatt
     3901 atcaaccaaa aaaatttgct gaaataggtg tagattttga agaaatcagt tcttgggaag
     3961 aatatatcca ggtggcaaaa gcattaaaag aaaaaactgg tagttttgga attgccttgc
     4021 cagaatctgg ggaccaagaa ttgtttatca acatgctagc ccagcagaat caatctttgc
     4081 ttgatgaaga aggaaatatc cgtctgaata ctaaagaagc gaaacatgct gcaaagttaa
     4141 tcaaagaaat gatcgactca gatattgtac atttctacgg tgcacaagat gctgagaaag
     4201 catttcaaga atctgcaatg tttgttgctg gcggctggta tgctacgaat atgtctttga
     4261 attttcctga tgcatctgac aaatggcgca tggctccact gattcctttt tcaaaagaaa
     4321 atccaggaaa aggtgcagta tcaggaggat caagttttta tgtccctaaa gatgcaaaaa
     4381 atccacaagt ggcacaacaa tttcttacat ttatgctgac agatgaagaa tgcttagcca
     4441 acgcactgga gctgggcgtg gctacatcta actcattggc ctatcaaaca gaagcagctg
     4501 agaaaaagtt tgactactaa ggaggacaaa aatactatga actattgaat aaaatcaatg
     4561 aaaatacatc aaatactgtt ttaccagcca cttattcaga tgctacgtct tatttgacta
     4621 ccgctgcata taactattgg caaacaggtg attttgaaaa ttcatatcta aaagaagcag
     4681 aaaattttgc tcataagtac ggcgtaaaat taactggtga ttaaaaaata ttaggaggac
     4741 ttaacgatga tgcaatcttc gttaccaagc aagacaataa agaacagcaa gaaaaaagca
     4801 acagatacgt ctcataaaaa aagtgcttat ggattggtca caccttttgt tattttcttt
     4861 attgtgacga ttttgattcc catcattatc tcgtttgctt ttagttttat gaaaattggc
     4921 tactcatttg aatttgttgg atttgacaat tatctagcta tcttccgaga tccattgttt
     4981 attaaatctt acacgaatgt tttgattatc atggcaggat caattccaat aacgatgcta
     5041 ttagctattt actttgctgt tttattgaat tcagcacagt taaaaggaaa aggatttttt
     5101 agaactgtct attatattcc aaccattaca tcaacggtgg ctgtagcttc cgtattcatg
     5161 acttttttca atccgactgg gttattcaat ggatttttga atgcgctagg aattaacggt
     5221 gtacagtggt taacaaatcc tttttggata cgaatatcaa tgatcatcac gatgatttgg
     5281 cttagtgtag gatataacac tattttattt ttagctggtc ttcaagggat ttctaaagag
     5341 atatacgaaa gtgctgaaat agatggagca tcgaaatttc ggcaattctt tttattaacg
     5401 atccccatgc taaagccaat cattttaatg gccacagttt tagccactat caatggcttg
     5461 ggtaatttca atatcccaaa tatattcttt gggacttcca atggaccaga aaattcagct
     5521 ttggtcgtag gagtcaattt atataaaaca agttttgaaa tggttgattt tggaaaagcc
     5581 tctgctattg cctggacgat ggtgtttgta tctgttatat tatcggctat tcaatttaaa
     5641 ttcggaggtg ataaaaatga ctaaacaaaa gatacaaagc agaattttta cgtacgtcat
     5701 tattgtgttt ggtgccttaa tatttttgat gccatattta tatatgattg tagcttcaac
     5761 gcaaagtaat caagaaattt taagtggcgg aatcaatttc aagtttgggg atatgctatt
     5821 aaaaaactgg caagccatca tggcgagata taattatccc ttgattattt ggaattcgat
     5881 tgttattgct gtaatcagta cagttttagc tacagccagt tctacaatgg cgggttatgc
     5941 attagcaaag tatcgtttta tggggaataa actgttgttc aatacagtta tgctatctag
     6001 gatggtgcct ttttttacca cattgattcc tttattttac attatgagtc gtttaggatt
     6061 atcgaatact tatgcaggtg tgatcatccc ttcgattgcc agtacaactt cagtgtttat
     6121 gatgcgacaa tatagcagtc aatttcctac agcactgatg gaggctgcca gaatcgatgg
     6181 tgccaatgag tgggtgattt ttacgaaaat cgctgtaccg attttatcac ctacgattat
     6241 tactagtggt ttacttatct ttatgggaag ttggaatcaa tatctatttc cattagtcat
     6301 gctttcagat aaagataaat atacggttcc cttaattatc aaaaatatga cggtttctag
     6361 tacaggtgag ccaattaatt atggcgcaat tatgctaatt ttagcaactt cagtgatccc
     6421 aatggtcttg atttatgtgt gggcgcagtc taaatttaag gagaatgaca ttggagctgc
     6481 aaataagtaa aaaaaataac gaatgttaac catctagttc gttctaggag caactccaca
     6541 ttcataaaaa ttaaaagaat ggcgggatat ccatgaatga acagacgaag gaatggtcaa
     6601 gaaaaatttt gaatgtagct tctgaaaaga tagatgcgga attagacaga atggggaata
     6661 aaattccata tatttcttac aacggaaaat atacagacgt aataaaggac agaggaatcg
     6721 actggtggac caatggtttt tggagtggta ttttatggca gctctttcac aagacaaaac
     6781 gagaaaagtt caaagaggca gcaatacaac aagaacaatt attagaatct gccctgtacc
     6841 atttttcaga cgttcatcat gatgtaggtt ttttattctt accatcggct attgcacatt
     6901 atcaacagtc tcaaaacaaa catagtcgac aactagggtt acatgcagct acggtacttg
     6961 ctggacgcta taatatttca gggaactatt tagtagcatg gaatgaccat aaacctggtt
     7021 ggatgattat tgatagtatg atgaatttac cattactgta ttgggcttcg agggaaactg
     7081 gtgatccgag gttctctaaa attgctcggc agcatgcaga tacaacagct aagtatctag
     7141 tgaggaaaga tggatcaacc gggcatatcg cagaatttga tgcagaatca gggaaatata
     7201 ttcggttact tggcgggcaa ggatacaatg agaattcttg ttggagcaga ggaaatgcat
     7261 gggctttata tggattcgcg ttaagttatc gttacacaaa aaatccgtac tatctagcta
     7321 tagcagaaaa gatagcagta aattttattc agaaaatcga aaaaactggt tatattcctc
     7381 tcattgattt tgctgtaaaa gattcgaata ttgataccag tgctggagta tgcgctgcat
     7441 gcggtcttct ggaattagca ggatatgttt cctctgtacg atcaaaaatg tatcatgaag
     7501 ctgctatcaa tatactccaa aagacagaaa agcactatgc aaattggaat atggatgaag
     7561 atgggatcat tggaggcgga gcagaagcat accatagaga actgacttat aatgtgccac
     7621 tgatctattc tgattatttt tttattgaag gcttacttcg attagaacag tcggaattag
     7681 ctatttggta gtaaaaagag gagaaaatac aatgaaacat ccagtaccac tattaaggac
     7741 cagacaggat tactttgact tatttaaaaa gataaatcaa ccactccagc cgttttatcg
     7801 agcaagtggc gcacaaattg attatataca tcaaggggta ggatatggaa ataaaattgc
     7861 aggaatggaa ggattctctc gggttttatg gggagcgggg cccgcaattg accaattaga
     7921 tgaacgatgg ttgaaattaa ttctatcagg aataaaagct ggaacagatc cttcccatcc
     7981 cgattattgg ggagaaatac atgaaagaga ccagcgtatg gtagaaatgc ccgcaatgtt
     8041 attagcgtta tatcatcatg aacaattatt atggaaaaaa ttagatacag gagaaaaaag
     8101 ccagattatt cattggttct cgcaaatttt tgaatatgaa tgtgcagatg gtaattggca
     8161 attttttaag ataattgtcg gaacaattat ccaaaaattt ggttatccag ttaaagaatc
     8221 agcactgaaa gaagcatttg ataatataga aaattgttat cttcaaaatg gctggtatcg
     8281 agacagttca agaggtcgcc aagattatta taatcctttt gcatttcatt attatggttt
     8341 gatttatagc gtactagaac cagaaaagcc cattagccag atatataaga accgagctat
     8401 tgatttttct agagactata ttcatttttt tgcggaagat ggtgcaaatg tgccttttgg
     8461 tcgaagcatg atttatcgtt ttgcagtttc aagcttttgg tcagctatgt tgtgggccga
     8521 tcttttgcct tttaaattag gtgaaatcaa ggggttagtc aatcggaata taagatggtg
     8581 gatgcaaaaa gaaatatttg atgaaaaagg tatcctgaat attggttata cctaccctca
     8641 attgacattg actgaaccct ataatagtac gttatctcca ttatggctga ataagatttt
     8701 tttattgttg gcgctacctg aagaccatga gtattggacg gctaaagaaa tgcctatgcc
     8761 tattcgtgaa ggaactaaac ttctttcaga agccaatttg ctcatacaac atgataatgg
     8821 acatacattt tttttgaatg cgggacaatt agggccaaat ttccatacat tgaccaatga
     8881 aaaatattta aaatttgctt attctagtca atttggtttt tcgataccgc gagcgaatca
     8941 attgaaagca gaaatggcaa tggattcaat gctagggatt cagcgagcgg acacatatat
     9001 cacgaccagt tttaagaatg agagcatcaa aacctatgga cagttcatcg ttcgaaataa
     9061 tgtacgagac attgtatgta agaaagaggt acttgcctct acatgggaac ctactttaca
     9121 ggcaaaaatc agaacttggc ttattccttt tgaaggctgg cagattcgcg tacacaaagt
     9181 agaattagat acagaatatg ttctatatga gacagggttt gctattgaat gttctcctga
     9241 gaaagaagga ataatcatag aagaagacaa ggaaaactat agggtgagtg aagctggctt
     9301 ttcaggtatt atctgtttaa gtgacgatac tattaaacga aaatccacag ctattatggg
     9361 tttgcccaat acaaacttga tgacatggga aaacactttt attcctggat tagagggtac
     9421 atttggaaaa ggaacccatt ggctcggaac aggagtggtt gctcatcaag atcgggatta
     9481 tagtttagaa gtatggaaca atcgtcccaa aattgatttt gatgggacaa ccgggctaac
     9541 tatccaaaat aaaaacaata aaaaaagact aaaattatgc taaaggctgg tggggaaatg
     9601 cttgataaag tgatgtttga agagataata catccagaaa aatggataaa tgaatgttat
     9661 aaagaattaa tggaaaaaga atggaataat gagatcgagc gttgtcgcag acacacggag
     9721 aggttaatca aacgtatcag taacagtcag tatcaattcc caagcgagtg tcctataaaa
     9781 ggacgttatc cattaagaga caatattggt tggacagaag gattttggac aggaattaat
     9841 tggttgttat ttgagaagac gggtgatcct ttatttcaac aaacagctga aaaagcagat
     9901 atagatttta tccacaggat tgactgtcaa attatcacta atcatcacga tcttggtttt
     9961 ttgtattctt tatcaaccgt agcagattat aaaataacgg gaaatcgaaa aatgaaggaa
    10021 gcttcaattg atgctgcaga aacattactt cagcgttttt gggagaaggc aggagttata
    10081 caagcttggg gagatcctag tgatccttca caacaaggta gaatgatcat ggattgttgc
    10141 atgaatcttc cattattgta ttgggcgagt gaagaaactg gaaatcaaaa atataaaaaa
    10201 gctgctcagg ctcatattca aaatgcagca gattatttag taagagaaga tggttcaact
    10261 tatcatacat tttatatgga tgtcaaagat ggaaaaccaa gatatggaag tacgcatcaa
    10321 ggttatagtg ataattcctg ttgggctaga ggccaagcat gggggattta cggttttgca
    10381 ttaagttatc gttatttgaa agaacaaaaa tattttgatt taagcaaaat attagccaat
    10441 tattttatga atcggttgcc agaagatacc atttgttatt gggatttgat ctttgatgat
    10501 tctagcagag aagaaaaaga tacatctagt atggtgatca tggtttgtgg attattagaa
    10561 ttaatagaat caggtctatt agacaaaaca gaaaagaact tataccagtc tgctgtttgg
    10621 aaaattatgc gacaattgaa agaaagatat gctacaacgg aagaagaaga tggcttttta
    10681 acacagtctg tttataataa gcccaaaaat aatggggtaa atacaccttg tatttggggt
    10741 gattactatt atttagaagc tttagtgaga atgactagta attggtcttc ttattggtga
    10801 aaaaggtaaa tatggaattt ttgtttgata aaataaaaaa agaaaaacca aaacagcatg
    10861 aatggtttct gttttggttt tttcgttaat tttcatattc ttctaattcc aagcttagtt
    10921 cttcccattc cgtcaatttt gtttcttgat cttgtctagc agcttctaaa tcttggttca
    10981 gctcattgag ttttacgtga tcttccaggt tttctggcgc gaccatttct tgttccaact
    11041 gatcgatcca ttcatcgatc cgcgccattt cttcttcaac ctgagtaatt tttcgttgaa
    11101 ggctacggat aagcttttgc tgttctttgc tttggtaata atcagatttg ttcgtgggag
    11161 caggagcaga ttctggctca tcttgtgcca acaattcggc gatttcttct tcttcctgct
    11221 ttttctctaa ataataatca tagtctccta gatagacttt gctgccattt tcagagagtt
    11281 cgacgacttt tgtcgcgatc cgattgatga aatatcggtc atgggaaaca aaaagcagtg
    11341 tgccgtcata atcgatcaat gcattttcta atacttcttt gttgtcaata tctaaatggt
    11401 ttgttggttc atccaaaatc aaaaaatttt ctttgttcat cgctaacttg gctaaagcaa
    11461 cccgtgcttt ttcaccgcca cttagcagag ggattggttt ttctacatct tcgccagaaa
    11521 ataagaaagc acctaaagca cttcggatct ctttttctgg agtggtagga tgctcatccc
    11581 acaattctgc taggatcgtc ttgtttgtat gcagatcagc ttgaccttga tcgtaatagc
    11641 cgatggtgac atttgtccct agcgtttctt ttccacggat aaaagggatc tggtgaatga
    11701 ttgatttcag caacgtcgat ttcccgatac cattcggacc tactagagca atcgcttctt
    11761 gacgacggat atctagattg attggttcag ataaggtgac attatcatat ccgatagcgg
    11821 catcttcgat ttgcaagact acatttccag aaggcttttc tatactgaat agaaagtttg
    11881 cttgtttttc atccccttga ggacgatcca gccggtccat tttttctaat gtctttctgc
    11941 ggctttgcgc tcgttttgtg gtagaggcgc gaacaaggtt ccgagcaacg aagtcttcta
    12001 atttatggat ttctgtttgc tgcttttcat aagccttcca ttcacttgct aactgttctg
    12061 ctttcaaatc tagatatttc gaatagttac ctttgtaatg gcgcattttt cttctgctta
    12121 attcatagac ttcattgact actttatcta aaaaataacg gtcatgggaa acaatcaata
    12181 atgcaccagt atagctttgc agataattct caagccagga aagtgtctcg atatctaaat
    12241 ggttcgttgg ttcatctaga atcaaaatgt cagggtgttg caaaagcatg cgagctaaag
    12301 ctaaacgtgt gcgctgtccg cctgataaag tatcgatttt ctgatcataa aatgaagaat
    12361 cgaatttaaa cccatgcaat acggaacgaa tttcattttc ataaccatag ccgtttcgat
    12421 ctgaaaaatc gtgttgcagc cggtcgtatt cttttaaata agaactataa gcaggtgtgt
    12481 ctggcgtacc ttcgctaatg aaaatctcta gctcgcgcat ccgctcttcc atttgtctaa
    12541 cttgttcaaa agctttcagc atctcgttcc aaactgtttc attggaatca agtcctgtgt
    12601 cctgagcgag gtatccgaga gtcgctgttt tatttttagc gatctttcct tcatcaggtg
    12661 cttcaattcc agcaatgatc tttaataaag tcgattttcc agcgccattt ctgccaacta
    12721 aagcgacacg agcgcctgta ctgatttcta aatgtatgtt ttcaaataag acttctgcac
    12781 caaaatggcg agcaatctga ttggcttgta ataatatcat ggaatcctct tttctacttt
    12841 gtgaattctc ctcaaataaa aaactatttt tcaattagta gtctaccata aattccatta
    12901 tactgcgaga tttgcagaaa tgataaataa atgaataaag aataaaattt cacaatatct
    12961 gagcaatgga attgattttt tcctataaat actgctatga tggagaaatc aaagatgtaa
    13021 ttagacaata cgtggaggtc atttcgtgaa agatcatatt attccaaagg cgacagctaa
    13081 aagactgcct ttgtattatc gctatttaag gattttaaat aatgctggaa agaaaaaagt
    13141 gtcttcaact gaattaagtg aagccgttca agtagatagt gcaacgatcc gtcgagattt
    13201 ttcttatttt ggcgaattag gaaaacgtgg gtatggttat gatgtagaag atcttatgca
    13261 cttttttgca aagacattaa acgacgatca attgacaaat gttgcgctga tcggtgtagg
    13321 gaacttggga agtgctttac taaaatacaa attccatcaa agtaatagca tacgtatcag
    13381 ttgtgctttt gatgtgaatg aggaaatcgt tgggcgcatc gtagacggta tccccgttta
    13441 tccaatggaa gatatgtcag aacaaatcaa gatccaacaa atcgaagtag caattttaac
    13501 gatacccgct gaaagagcac aagaagtagt caatcagtta acggaagcag gaatcaaagg
    13561 aatcttgaac tttaccgctg ctcgattgaa tgctccgtca gatgtattga tccaaaatgt
    13621 tgatttgacg aatgaattgc aaaccttgat ctatttcttg cattctgacg caaatgcgca
    13681 ttccgaaaat acagcagaat aaaaataaaa agcgattgaa caaatgaagg caaaactttc
    13741 acttgttcaa tcgctttttt ctgtttggac aacgattatt ttgttcggaa gatttcttca
    13801 taagaaaggg aatcatcttc tgctgtcagt tcatctagta gataacggat ttttttaaag
    13861 tcgatgaacc gtgcttgccg aaagacttct tttccttgat gaaaaatcag gacaactggc
    13921 gcagtcagca cttcaaattt actagctact tctggtgttt caaatgcatc caaatgaagt
    13981 gccggaatat cataatgtgt gagaagttcc tctagtcgtg gacgaacagc gtgacaaaca
    14041 ctgcaattag gcatcgaaag ataaatgacg gcaacggatg actgttgcag tatgtcttga
    14101 acttcagtca agttagtggt ttgtttcatt tttattctcc cttttatgat agaagtgata
    14161 tgtatgagac cttaatttaa tgggaataac agaaaaagtc aattgaagtg ctttggtaaa
    14221 aaataagagt tgtgtaaaaa tttacagagc aacttgtttt ttttgaataa aagcgtatat
    14281 ttgcaaagcg aatgaactag aaataaaaag gaggaaactc atgttttctt ttattccaaa
    14341 ttcggttttg cagatgagta cgattttctt atcgatcgtt attgaagcat tacctttcgt
    14401 attattaggc tgtcttatct caggcgcctt acaagtattt ttgaccccag aacgtgtgaa
    14461 gagatggctt cctgaaaatc gtatcttctc tatttttact ggttcgatct taggattttt
    14521 ctttccttca tgtgaatgcg gcatcgtacc gatcgttcat caatttgtta aaaaaggcgt
    14581 accagttcac acagcgtttg cttttatgct gactgctccg atcatcaatc caatcgtgat
    14641 tttttccact tttatcgcat ttggcaattc ttggcagatg gcaagctggc gggtgatagg
    14701 cagctttttt gttgcccttg tgattggtgt ctggcttgcc tattttcaaa aagacgctgt
    14761 attgaaagaa aaaatcacgt ctaaagtagc agaaaatcac catcatcatg tccatgaaga
    14821 agaacaaagt gagaagaatg aaccaaaaaa tcagctgatc catcaaactg ggcatgtgtt
    14881 gacacattca atcgacgaat tttttgatac aggtcgttat ttgatcattg gcggattaat
    14941 cgcagctgcg atgcagactt atttaccaac tagaattatg ctaacgctcg gctcaacaaa
    15001 gattttagcc attatcatca tgctgttatt ggcctttacg atgtctcttt gctcagaagc
    15061 agatgcgttt ataggatctt ctttgttaag tctgttcggc acagcacctg tagctgcctt
    15121 tttggtcttt ggcccaatgg tcgatatcaa aaatctactg atgatgaaac gatattttac
    15181 aggacgcttt attttatcgc tgatcggact gattgccctt agcgttattg gattcacatt
    15241 atttatttag gagggaaaat atgctgcggt ttttgatttt atctgggtat ttcgaattga
    15301 tgatgtactt gcaagtttct ggcaaactcg atcaatatat caatgtccat tatcgctact
    15361 tagctattct ttcaatgatt ttatctgctc ttttggcttt agttcagctg tatttgtggg
    15421 tcaaaaacgg aaaagcagaa gaaaaggcac acgagcacga tcatgaccat gggttgtcaa
    15481 aaccttatca acgtggaatc gcttatgtac tgctagcttt gccagtaatc gtggggacgt
    15541 tgtttccaac agttagtctc gacactacga tcgtagaagc caaaggattt aatttcccag
    15601 taagtaaaga atcagtaggg gatccggaga tgcagacaca atacttaaag ccggatacca
    15661 gtatttattt caacaaatca gactacttag accaaatgaa tgagttgaaa gaaaagtatg
    15721 gggataagca aactatcaag atcacagatg aaaactattt agaagttatg gagctaattt
    15781 ataattaccc tagcgaattc attggcaaaa aaatcagcta tgaaggattt gtttaccaaa
    15841 caccgaacga tagcaataca gatatcttct tgttccgatt cggcattatc cattgtgtgg
    15901 cagattcagg agtctttggt ttgttgacgc atatgccgga aggagtaacg gttaaaaatg
    15961 atgaatggta taaaatagaa ggaaccatcc aatcagacta ttatcaacca ttcaagcgtg
    16021 aaatcccatc tgtagttgtt acaaacgctg agaaagtctc tgctccgaaa aatcagtatg
    16081 tgtatcgttc tttttaaaag aacaaaataa ataaacatag ctcagacagc tgacacgcaa
    16141 ggacgtcggt tgtctggctt tttttgcatg atgtattcat ttgtttgata tttattttag
    16201 aagcagcatc ctgaaagtat ttgatatcgg tttcaaaggt gttggaaaag attgtgaaga
    16261 gaattacaat ttattcctaa catgtatgtt gaaaagttgt atgaaatgat tttgatagta
    16321 aaatatcgag tggaggtact ttttatggta acgcgtatat ttattttgga tgatagtttg
    16381 gtttttgaaa aaatgctagt agaaattttg gaagaatcca gaattttatt aagtccttgg
    16441 aaatttgaga tcgttcaggg attgaacgtt atacaatttg ttgaattcat taaaaataat
    16501 gaaatctatt cctcagatat ttttttccta gacatcgatt tgaacgccta ttatagcggt
    16561 atcgaactag gcgaaatgct tagagaaaag aatgaaaagt gtaaaattgt ttatctaacg
    16621 tctcatccga ataaagcaat aactgttatt aatcgaaaga ttacgcctag tggttatttg
    16681 ataaaagaat tcacttatga aaaaaataaa aaaagcatcc aaagttactt attgaaagaa
    16741 aaagaaaact atcctgttca acatgctacg aaaaaaaggt ggacaactgt ccgttatggt
    16801 aatagcgatc actatatcca ttacgatgaa atcctttatc taatgagcat gccaggttat
    16861 aaaaataaaa taggcctagt attgaaagat gaagagattc tcgtaaacgg cacaattaaa
    16921 aaatataaaa aatcactcga atatgagtat ttatgtaaag atttaaaagc ttatatcatt
    16981 aatactgaaa acattacatc tattagcaga gtaaacgaaa tagtaacatt tgcaaatgga
    17041 gaacaactag agatgggaaa gagaagtatt gataaactaa agaattttat gcaagaaaag
    17101 atagaaaatg aatcatttta aagaaaacaa agactcatat cttacctgtt agaagagtgt
    17161 tttcctgtag aagagaacaa cccaagtagc agcctagtcg tttctatcct tttttgttat
    17221 gcactctttt tgacatttac tccaccaata acgaagggta caataaaaag taataacaaa
    17281 caaaaggagt acagaaatga ttgaattcat caatgtaagt aaaagttacg ggaaaaaaca
    17341 agcattaaaa gacttgtctc tctcgataag acaaggggaa atttttggat ttttaggaca
    17401 taatggtgcc ggaaaatcaa ctacgattaa aagtttagtt agtattattg aaccatcatc
    17461 gggaacgatt ttagtagatg gaatgaaatt gacggaaaat cgattgagta tcaaacaaaa
    17521 aatcggttat gtgcctgatt ccccagatat ttttttacaa ttgacggctg gagaatattg
    17581 ggacttgatt tcagctgcct atgaattaga cacacaaaaa aaagaaaagc gtttagcaga
    17641 attgacagcg ctttttgata tgtatagtca tcaaaatgaa acaatcgcaa gtttttcaca
    17701 tggaatgcgt caaaaaacga ttttgatcgg cacgttgctg ccagatccag atatttgggt
    17761 attggatgaa cctttgcaag gacttgatcc tcaagcagca tttgatttga aagaaatgat
    17821 gaaggctcat gccgcaaaag gaaaaacagt cattttttct acacatgtat tggataccgc
    17881 acaacagtta tgcgataaac tggctatttt gaagaaaggt gaattgattt atcagggggc
    17941 agttagtgaa ttattgaatg gctcacctga tgaaacgcta gaaaagattt atttgaaaat
    18001 ggctggaagg caagcgacag gagatggacc gtatgtataa gcgacaatta ttagaattaa
    18061 ttcgagttaa tcttttatat gttaatccac agctcacgaa taaagcaaga gagaaaggaa
    18121 aaaagggaaa gcgattaact atctatcttt tgtctcaaaa tcttttttca gggctgattt
    18181 ttttactgat ttacggattt gctatgtttg ctatgaactt tgttaaattg ccaggtttct
    18241 ttacttatta cgtggcactt tttggtatgt taggtttaac tcaaagtata tcagtcattt
    18301 ataatatctt ttttgaagga aatgacctgc aagtattttt gcctctgcct tttggacaag
    18361 aacaggtttt ttcagcaaag attttggtag ttgctttgac catcattcct tttgtttttc
    18421 cgcttctagt catgtttatt ttaaccggat ggcgagctga tctagggatt ttgcttacta
    18481 tcattatctc aatccttttg ttttctctat ttttgattat tcttttttgt ctggctagtt
    18541 tgattgtttt tggtttggct cgaacagcag tgtttaaaaa acatagaaaa acagtttcta
    18601 gtatcttgct tggcagctcg atgctgatcg catttgctgg aatcatgtgg atgaatcatc
    18661 aaacggggac tattgaaaat gagttaacag atcggcatcc gatttcgata ttattgccat
    18721 tctatgatgc tgccagtcag ccactttctc aacaaggttt attgggttgg ggaatactag
    18781 ctatcacggc tctgggattt ttccttgctg taaaacagct gatagtacca aagcttacag
    18841 agcaactgac tgattcaaac agtgagttca aaataaaaag aacccataaa aagaaccaaa
    18901 atgtgcatca gcttttattt agttacaatg ctcaattagt aaaagaacct aatttgatta
    18961 tgcaagttct ttcttcttcc ttgttaactc caatcatttt tattcttgct tttgccctag
    19021 gaggcgaaat aaaattaact gatttagata ataagttaat cggtgttgtt ttcctggtag
    19081 ggatcgcatt ggctttctta acagttaatc agacttcttt tatcagcaat cttatttctt
    19141 tggatcaaga aaattttctg tttatccgtt ctttgccaat ctctatgaac caatatttaa
    19201 aagaaaagtt cagattcgga tggatattgc agagtttttt aactggtggg atcgcattat
    19261 taagcggact gtcttttcag ctgcctttgt tttttatccc tagcttgcta tttggttctt
    19321 tattcggctg ctacttattg tcactacgtt atttcgctag agattatcgg ttgctgctgc
    19381 tgaactggac caatattaac caattattta acagaggttc aggaagtttg ggtttagtcg
    19441 caactatgat gggttcattg atcatcagta ttattcttat cgtcttatac ggaatgttgg
    19501 ctttgtttta tccattttgg ttagtgaata ttcctgtgat cggtttagtt ctaatacttt
    19561 ccgctctatg gatcgtttat tatcaacgaa cgttttggaa aaaatttgaa taaagaagag
    19621 gggccgtgac aaaaatcatg tcgcagcccc ttttttacag atagagggtg ttcaaaagtc
    19681 agtttctcag tattaagtca attttatgta aaaatgtgag aagctatttt tacaaaactt
    19741 tttttaatac tcataattac ttcttattgc tcctgcggtt acttgtcgca atgtttgtga
    19801 gatattttgt catcgctcaa ggctgaacgt ctctttcaca accttttctt tgttacactt
    19861 cgtttattta aaaagccgtt tttttcgttt catcatgatt gcgtcacgct ttactttgaa
    19921 attacggatg ttatggttaa agacttctga taatacaaga cctaaagcga ttgctccagc
    19981 aatcataatt gcttggaccg cataatgagc cgcttctcga taggattcag taacgagatt
    20041 tcgaaccgct tgataagcta aaccaccagg gactagagga accatcccgg gaatattgaa
    20101 aatcgttacg ggcattttca aacgtttaga gaaaagatcg ctgatgaatg ctactcctaa
    20161 agaaccaagc aatgaaccaa aagcagcaga tccgcctatt tgaacacaga tccagtagat
    20221 catccagcca aaagcaccag aaagaccaca acaaatcaat gaacgcctag gtacatttgt
    20281 aataacggca aatgccgcag tcgctaaaaa actaaaagaa aactgaataa atatcgtccc
    20341 catacttgtg ttcctttcct aataaaaaag ctggaaaata aaagcaatcg caaaaccgat
    20401 catcgtagca gtcatcatcg cttctgttcc gcgagaaact cctgagacat aatgtccagc
    20461 tagcaaatca cggatagcgt tcgtaatttg aactccagga actaaaggca tcacacatcc
    20521 gataatgatt agatcctgat tgcttcctaa gccaaaacgt acgctgtaga tagcggctat
    20581 gccaatcaat aaagaagata aaaattccga taaaaatttg attctaaaaa atttcaaact
    20641 atatagataa gctgcgtatc ccaatcctcc aatgactaaa gtcacaggta aatcggtcat
    20701 ctttccgcca aataacacca taattgttcc actgatcact gccgcgctaa cgatttgcaa
    20761 ccagacagga aagaaagaac ggtctcggtc gattgcttgc agttcgcagt aaagttcttc
    20821 taatgttagc tctgaagcaa catatttacg tgaaagatca tttactttta ctactttttc
    20881 taaattaatc gaacggttgg taatttgttc cattcggatg gtcgaggtac gatcaagacc
    20941 aataaagaga ccggtttgag tgacatagct gaccaaacgg aaatttccag aagctaaagc
    21001 aattcgactc atcgtatctt ctacccgata catctccgca ttgctttcca tcatgatttt
    21061 cccagcgagt aaacaagtgt ccagcaacag atctaattct tttcccgtca tagcatcttc
    21121 tccaataatt gattgattag ttttatttta ctacaaaaca taccagaagc gtaaagaata
    21181 aaaaaacttg aggaaggaat aaaagaggcg tatactgttt ttgtattgcg ggtgtagttt
    21241 agtggtaaaa tcccagcttc ccaagctgat gtcgcgggtt cgattcccgt cacccgctta
    21301 aaataggttg tgaaaaagct gctctgcaac aagtaataag aacagccaag caaaaacaac
    21361 ttcttatgtt tttaacttgg atggacttat taccgcagat gcaagctttt gcgcaccgtt
    21421 taccaagcgg ttgtgaaatc agtgtattgt cttgagtatg aagaaaattt ttgaataata
    21481 ataaatacct tacagataga gtggtgactg attagagacg aggaatccgt ctcttttttg
    21541 tatttttaag ccatcttaaa acaggtttta ttttttgctg tttctatttc tttctagtat
    21601 ttatttatag gggaacatat gttaagattt agggagctgt tagtaaagga gtaaagaaat
    21661 gagcaaaaaa acaaccaagg cgatcgtggg gatcgtcgct gctgtgattg caggggcatt
    21721 cggtgtttcc accacacaaa aagattcaga tatctttcag cagattacaa ctatttttga
    21781 ttccacacca caagcttctc aagtagcgga tgtgccagaa tatgatggaa aacatcaaga
    21841 aatagaaatc aatgggaatc agccggaatt tacaaaagag gatcttagcc tttccaaagg
    21901 aacatgggaa agctattcaa atattgataa attgaacaga gtaggacaag ccaatgctat
    21961 gcttggaaaa gatttgttcc ctactgaaaa gagagagcct ttgtatattg acccgacagg
    22021 ttggaaacaa aagaaattaa gtgacgggca atggttatat aatcgcagcc atttaattgg
    22081 tttccagctg actggtcaaa ataacaacat taaaaacttg atgacaggga cacgttcgtt
    22141 gaatgctcca tacatgcttg ctcacgaaaa tgatattgcg gcatatatca aagaaacaaa
    22201 tcatcatgta cgctaccgtg tcactcctca ttttgagggg aatgaactgg tagccagggg
    22261 agtgcaattg gaagcagaaa gcattgaaga caaaaaaatt gaattcaatg tctttatcta
    22321 caatgttcaa gatggctaca cgatcaatta tgagacagga caagcaacaa aaacaaaaaa
    22381 ttagtagcag ctattcgaat tttttatgaa tattatgaaa aaattgtgta aaaacataca
    22441 ttcaaagaaa tatagcgtat actttatgaa aagagtactt ttgataaagc tacagaaaaa
    22501 aggaaaaaaa catacaaata gtgtttctta atttattcgg aagtttggaa tatttgtttg
    22561 tagttttccc tttcttcgag taaaataagg ttgtagaagg taagctggaa ttaccttgcg
    22621 ggaaagaggt gtagaatcat gggttttaca gatgaaaccg tacgttttgg ttttgacgat
    22681 agtcgcaaaa aagaagtgag cgaaacatta gcgattgttt atcgggcttt ggaagaaaaa
    22741 ggctacaatc caatcaatca gattgtcggc tacttgcttt ctggagaccc gtcctacatt
    22801 cctcgttatc gtgatgctcg aaatttgatt cgccgtcacg aacgcgatga gatcatggaa
    22861 gtaattgtca aggactacct ctcaaatcat ggagtgaatt tatgagagta atgggcttgg
    22921 atgtcggttc taagacagta ggtgttgcta tcagtgaccc ctttggctgg acagctcaag
    22981 gagtagagat cattcggatc aatgaagcag aagaagagtt tggctttgat cggttgaagg
    23041 aattagtagc acaatatgaa gtagaccggt ttgtagttgg attacctaaa aatatgaaca
    23101 attccatcgg accaagggca gaagcttcca tgtcatacgg agaaaagatc aaagaactat
    23161 tctccttgcc agttgattat caagatgaac gattaaccac tgttcaggca gaacgtatgc
    23221 tagtagagca ggcaaatact tcaagagcca aacgaaaaaa agtcatcgat aaattagcag
    23281 ctgtgatgat cttacaaaat tatttagacg gctcaggtaa gcaattattt taataataaa
    23341 agaaaagagg taatcttatg acagaacata accattccca cgaccatgaa ggacacgaac
    23401 acatcacatt ggtggatgac caaggaaatg aaacattata tgaaatccta ctaacagttg
    23461 acggacaaga agaatttgga cgtaactatg ttcttttata tcctgctggt gtttcagaag
    23521 atgaagatgt agaattacaa gcttatgcat acatcgaaaa cgaagatgga acagaaggcg
    23581 agttagaaca aatcgaaaca gacgcagaat gggacatgat tgaagaagta ttcaatacat
    23641 tcatggcgga agaagaagaa tagactataa agctaggaga aaccttgata tgatagggtt
    23701 tctccttttt ttatttttta ctgtatgcca tattgtatgc catctagtca atatttaagt
    23761 gttgtactaa tttttcaaca gtcttttctt tcgtttcttt tgccaagtga ccgtaacaat
    23821 cgatcgtctg actaattttt gtatgccgta atctagtttg aacttctacc atagtagctc
    23881 cagcgtccaa gagcattgaa gcatgagtgt gtcttgctac atgcacaccg agaaatggaa
    23941 ccatagcttt ttcacaaata gtaatcaatc ttgcataaat agcgtggtgt gtgattagtt
    24001 ctttatagat accacaaaaa atcatagtcg gatcagttac accaagtgtc agcatatatt
    24061 tccgctgatt aatctgccat ttttttaata tagaaattgt tctttcatct agtggaagaa
    24121 ccccagcact acaatcagtt ttaggatcac tgattttata gccatttttg gattggctta
    24181 gtgttttatt gatggtgacc gtattttctt gaaaatcaat gtctgaccaa ttcaatgcaa
    24241 gggcttcact gatcctaacc ccgctataaa agagaaagcg tagtaacgtt ttatcaaagt
    24301 cgtttcgata agtttcaggt ttgctgttta ggtaatcaaa caaggtgatc agttgttctt
    24361 tactgtaaac tttgaccttt tctttatgct tagtggtggt tcttttaggc attaatacgt
    24421 tgtctagtgg attagtctca ataatcccaa gattgatagc gtagctaaaa atacgcttag
    24481 aagttgaatg aagtaccttg tattggtcag tagtttcagc ccagctattt acaatttgtt
    24541 gggctttttt taccgtgact tcattcacac gaagatttcc atattttgga agaatccact
    24601 ttctaaactt gatttcagtt ttcatgaagg tggcttctcg gacagtggtt ttatatgttt
    24661 ctagccacaa gtcataaact tctttaaacg tttcgttgtt cgctttttta ataccgtttt
    24721 tatcgtagtc cacttgcaag cgtgacaagg caagctgggc ttcttttttt gttttgtaac
    24781 ctcgtctacg aaccttatca ggtttgccag tcattttgtt cattccaaga taggcttcat
    24841 agagccaaaa tttacctttt ttagtttcat attgcttgaa tgttgccata atagttttcc
    24901 ctccatagtg tagcaagccg tgggggcggt tggaggaggc agtttattat tattttgaat
    24961 agtgtttatt tagctcagta acaagagctg tgaactcttt tagaagatca ttttcataaa
    25021 attcttgata ctcttttttc tcatctgtat ttttttctga atcattaatt aatgagtcat
    25081 atcgtaaaag cgtatttaga agagctgcga gataagcaat tggggtactc ccgtcaggaa
    25141 attcaattcg agatcctgtt ttaagatatt ttattgcgtt tgttaaataa atcttctgtg
    25201 agacagtcag gttatcttct tcgatgttat tgatagcatt agaaagatta gaatcaatta
    25261 tagagttatt tttttcaata gtttttttca attgcttttc aagcacattt cgcttaattt
    25321 cgggcatcat atgcatatct cttaatgtat aagatccgtt taatagatag gtagtggata
    25381 catctaatac ctttgaaagt tgtttaagcc tggtgttatt gggaagatta tagccatttt
    25441 cccaattact aacggcacct ttactggccg gtggggataa tttacctcca agttgttcca
    25501 ttgagagtcc cttttcaatt ctagccagtc taattctttg tcctgtctcc actgcttcgg
    25561 ggtatttttt tctagccaca ttttcaccac cattcatata ttttctctac tatatcacga
    25621 tttaaatatg tgtgaaacat aaaagttcaa aaaagtatgt taaaattctt gcaaatgaaa
    25681 aggtatgatg gtatattgag tttataagag gtatagaaat gtattcaaga gcctcttgca
    25741 tttttttaaa atctttgtat agaaatgtat gctaacgagg ggggataaat atggaattaa
    25801 aaattgacaa actcacgctt gatgatccag cttttaaaat tattgacgat cgagttgttc
    25861 aacaagtaga gcaaactcaa ggaacgttac tttcattatt agaaaaaaag taccagtttc
    25921 caaaatatat gaacaaggca caagctgcta agtatatgaa cgtttcatac aatactatga
    25981 tgcagaaata tgttcctaac ggtttaaagt tgattatagt tgatggagtc gtaagaattt
    26041 cacaagatga atgtgatcgc tttatggaag aataccaaaa ataaacgcaa gccgtggggg
    26101 caaagggatt aataggagga aataataatg gaatcgacac aaaataaagc aatcgaaaaa
    26161 gtattatcaa caattcttac agaagacaca gcggcagaat tagcaaactt ggaaggcaaa
    26221 gcgctagaag aaacgtttga atgtttgtac gaacaaatgg actatcaaaa attgctacca
    26281 caagaaccaa cggtaagcgg agtattgcgt ggcttgaacg atttagtaca agcagagttt
    26341 aaagaacgac tttctattga agagtatcaa gaaattttgt atcagcaagt tgagttgtta
    26401 gcgagcttgc tagggcttga actggaggac gttgaatgaa aactgaaatt tggaacggac
    26461 acattataag atttgtcgat attaatgatg aatggtgggc agtggcgaaa gatgttgcgg
    26521 aagcgttagg tcttaaacag gttactagag ccattcactc tttgcctaaa gatggggtta
    26581 ctactagtaa ggtcatcgat tcattgggca gaacacaaga tgtaaacatc atcaacgaaa
    26641 aaaaatattt accgcatggc attcaaaagc cgtaaaaaag aagccgaggc atttcaagac
    26701 tggattttcg acatcatcaa agaactacgc cagtccacag gacttgaagg ctttcaagta
    26761 ttccgcatgc tagacaaaga acaccaaaaa gaagcaatga ctaaattaag ccacgctatt
    26821 actgaaccaa aaccagtaga ctacattaaa gcgaatgtga tagccaataa agctgtatca
    26881 actatttatg gtcattccaa aatggtaaaa aagaaagata tgactcctga aatgttggtt
    26941 gatcgtgaac ctattttaga tgagacagta gaattaatga ctgttaaaga aaaatatggg
    27001 ttgcagtttt cagtgagtga gaaaatttat aatcgttctg ctgaattgca gacaacttag
    27061 gagcattaac atgatcgaga acaaagaaaa agccttatcc ataacagtct tggcggacag
    27121 gataaaggca acttaattgg gataaaaccc gatattcttt gttccgatta taccatatta
    27181 aaaaatcgga gggaaccatt atgaaacgaa acttaattat aggaagcatt ttaatcggcg
    27241 gaattttcgg cttattacca ttaattgcaa aaatagctat ctttgcagga ttagcgttat
    27301 atctcttcac tgcttatgac gagtacgact atcaattaag aactggaggg cataagtaga
    27361 tgtacaaaac aaaactatta aaccaactcg atagccttga attagaggaa attaatcaag
    27421 ggatcgctga actagaaaat aatatcggga aaacctactt tggaaattca ttcaatgaaa
    27481 aactaacagt tttgtatgta ctcaaaaaac atgcagaaca caaaataatt tgtcgagaaa
    27541 tcaacgagct aaaaaatcag attttaaccg catggttaaa cattactgat atgcaagaag
    27601 caagggtgaa aacatttaat acatgggtaa agtatcagaa tcaattaaaa ggtgctgaat
    27661 ttgttcggga tgggttgaaa tacgaacttg agcagttgaa gctcatggag gtgtctgaat
    27721 gatttatacc agtattttga aaaaggagga gaggtttatt ggcaaataat tatcaagcag
    27781 caacggaatt acaaaagtcg ggatggtcaa tttatccctt atctcctgga actaaaattc
    27841 caataaaagg ctctagtggt tggaatgatg caactaacga ttctaatcaa attaatgaat
    27901 ggtggacgga agatgcaact agaaatattg ggttaatgct tgaagatcat caaatggtgg
    27961 tagttgacct agatcaacat tctgaaacac gaaacggcgt tgagaattat aaaaaacttg
    28021 cttcaatgta tgatccattt ccttctactt atacggaaat cacaccaggt aaaggaatcc
    28081 atttcttctt taaaattcct gatggtgtaa ttatccaaca agaaacaggt gcatttgctg
    28141 atatcttcgg gcgtgatgat aaaggtaaaa gtttaagcgg gattgatatt atcactaaag
    28201 gagttccaat agcgccaact atgattattt caagaagtgt tgggagagca tatgaaccta
    28261 ttgaaaacgt atctctaaat aatattgcac cagctcccga ttggctagtg aacctactgc
    28321 taaaaaaacc gcaagaaata aagactcaat accgacccgt agcaaaaaca gggacagcaa
    28381 aaaaattaga tatgattgtt caaggcgctg gcgaaggtag tagaaacgat catttaacta
    28441 agttatgcgg ttggttacta tggcacggtg tagataatca aactttagca gaattgattt
    28501 atacagcaaa tgcgtacaat gaaccaccgc taaaagataa agaagtgaat caaattattc
    28561 gatcgatgat taaaaaagat ttgaggggga aagctcatgg ctcataagca taaattagat
    28621 agtattttag actttcccga agccagcgag cgtgaagaca atatcattga attgaaaacc
    28681 tggatgtcac gattaagatg caataaagac gatcagatta aaagtaacag tgttgtgaat
    28741 gcagaattga ttttaaccaa tgatagcaat ttagctggaa caattgctta taacgaattt
    28801 agcggataca ttcacctatt aaaagattct ccttggatca atcgtaccgc tggagaatgg
    28861 gaagacagtt ttgaggatgc cttaacggca tatattgaag aaaactacaa tgtagttttt
    28921 gatgacaaca aaatacacaa agcagtagta aatgtggcta gaaagaatgt tttcaaccca
    28981 gtaaaagaac gtatcgaaaa agttaaatgg gatcaacagc ctagactgga aacactgttt
    29041 attgaccttt taggggtaga cgacaacctt tacacacgag aagtaacaaa acgttggata
    29101 gtcggatcag tcgcacgtat ctataaacca ggaataaagt ttgagatcgt tccagttttg
    29161 gatggaccgc aagggattgg taagagtacc gttccagcat tactttacac ggatgatttt
    29221 ttcaccgatt cattagattc gctaggtgaa aagaaagatg attatatgca gcttcaaggc
    29281 aatgtaatta ttgaacttgg agaattatct tcaatgaaca aaactaaaat tgaacagata
    29341 aaaaacttca tcagtgccaa gatcgataaa attagacctc cttatggtcg gaatgtcata
    29401 gcttgggcaa ggcaatgtgt atttattgga acgtcgaatg atgggcagta tttgaaagat
    29461 gatacaggaa accgccgttt ttatcctttg ccttgtaaga ataagccaaa gatgaatccg
    29521 tttaaaacaa aggatgaata ttttcttcaa gtgttagctg aagcaaaggt gctttttgat
    29581 aaaggacaac gaatctattt tagtcccgat gaagataaag aagtcttaga aattgcaaaa
    29641 gactatcaag aggatgccaa gatggagaat ccaattaaag aagcaatcac taaatatttg
    29701 gaaatggaaa tcccgtataa atgggaagaa gctccagcgt gggcgagacg ttcttattat
    29761 cagaactatc ctgacagtaa atgtaatgag aaaaatttac agcatttcta taatggagct
    29821 actatgcaac aagagtttta tttagttgat agcgtactca ccgctgatat tttggaagct
    29881 gtttttgata aacaagcaaa ggatttattg aatggtcgat cagatgcaga aactaaaaag
    29941 attgccttga ttattagcag tattcctggt tgggaacgga agcaactgcc aaaaagaaat
    30001 aaacgacgag gattttacaa taagtcaaat gcctatgaaa ataaaaatcg agctggaaag
    30061 tacaaaaagt aacgggtagt aacgggtcag taacacctag cccgttacta gtttaccctt
    30121 actgccccaa gggatacagc aatttggtaa cggggtaaca ccttatttcc ttaaaacttt
    30181 tgaaatagtt agctaatatg ctggggaata atgttactgg attttacccg ttacctgtta
    30241 ccaaaacgct agaacccttg tccccgttgg gattgagtgg taacgggtac ctgttacgta
    30301 ggtgttacca cgtgttacga atcaaggagg aaagtaaatg ctcaatatta tttcaacaaa
    30361 caaagcaccg aactttcaat atacagatga aatggatcgg tttctaatga acacgctggc
    30421 atttagtgtg ggattggtaa cagaagatta ttccacattt gatccagaag tattaaagat
    30481 aatggaagaa gaacctgatt ggctgcagga gtcagtagcg tggtgtcaat cgctagtagt
    30541 gggatcattg gtagatagtg gcaactatga tgatacaggt gagctaatgg atgaatttaa
    30601 ttgtctgctt aacctatatg atcgagcgag acaacgagag cttacatcga atgaggataa
    30661 tttgttttta aacatccatg ataagttctt ggctttgcta ctaacagatg atgaattgat
    30721 aactaattta ttggaggtag aataattatg catatggaaa gtattagata cttcgatggt
    30781 gagaaacaca ttgagtattc aaacactcaa gaagatgtag actttattag ttttgaggaa
    30841 gataaaatca ctaaagttaa ttttaaagac ggaacgtgct tgaagatagt ttcgccttat
    30901 attgaataca aatcaaaatg ggaacaagat gattctgacg aacgagatcg ctttggttgg
    30961 taatctatgg cagttaaaaa acaatgtaat catgctggat gtaaaacatt gattgattat
    31021 aggcagaagt attgtgggaa gcataaagca aagcaaacgg caattaagcg tgaagaaaga
    31081 aagcaaagcg aaggtaaata ttttcaattc tatcaaagta gaacatggcg aaaggcttca
    31141 tacttgtatc ggttaaatca tccagtatgt gaggattgcc tagaagaagg cttgataagg
    31201 aaagctgatg ttgtagatca caaaattgaa ctaaaagatg attggaatag acggttagat
    31261 gaaactaatt ttcgttcttt atgccatgca caccataatt ccaagacagc caatgaaaga
    31321 caaagacgag aaaagagtac cctttgagtt aagggggcat agtgtgaaac ttactgacaa
    31381 tcgatgccta ctcattttgg tacaaataac cgttgtgaaa aggcataagg gtaattacaa
    31441 ataacgatta cacttgtaat cacaagtgaa aaagtatata atgaaagtag gagatttatt
    31501 gaaaaataca attgttatta ctgataaaac agggaaacag cgcaccattg agttacctca
    31561 ttttggtcaa gtgaccattc aaatgcagaa cggaaaaatc atttatatag ataaattaga
    31621 taaagaaaaa ttctgatcga aaaacgaagg aatgtgagct taattgctta cgttcctttt
    31681 tcttttgtct gaaaggagga caacatggga caccctaaaa tattacacga tacaaaaggc
    31741 aatatatcaa gtgaagaaaa agcaatccgt gtggacgcta gagaagaatt gttcaagcag
    31801 caaccattaa taaatatcac gccccctgac tggatggcag cgagcgctag aagtgaatgg
    31861 aatcgtatcg taccaacatt aaaaaaagat tatccattga gtgaagcaga ttatggttca
    31921 ttggtagcct attgtttagc ctttgctcga atgaaaacag ccgaagccga gataagaaaa
    31981 tcaggtacgt ttatcacatg cgaaaacgga gtaaagaaag ccaatccagc ggttcgggtt
    32041 caatcacaag ctatgagtga tttgaaaaaa caagccacct cactaggtat gaccttagaa
    32101 tcacgatcaa aactagcttt gaacaaggtt aaaaatgatg aacccgaaga cccgttcaaa
    32161 gagttgatga attcatgaat gattacattg agaaagtcct atcaggtaag ctgattgcac
    32221 ccaagaaaat tatccaagcg tgtgagcgtc atataagcga tttggagcgt tctaagtcag
    32281 atagcttccc ttatgtgttc gatgaagaac aagccaccaa agcgattaaa ttcattgagt
    32341 tgctaccatc tacggacggt aaagcaatca agatgttagg atttcaaaaa ttcattctag
    32401 gcagtcttta tggctggcgt actaaagaag ggaattacag gcgattcaat cgagcgttta
    32461 ccagtatgag ccgtaagaat gggaaaacgt atatcgcaag tggcatggct gccaatgcgt
    32521 tgattatgga acaagaacca gcagaagcaa ggcaagtatt gtttgtaagt aacgctttga
    32581 aacaagctaa attgggctat gatatgctgt ctaattcact tagaaacgtg gtcaagtcta
    32641 gtaagttttt aagaccacaa ctgaaaatta tgaactctaa gattcagcac ttgccctcta
    32701 attcgttcgc tatggcactg gctagtgaaa ccagcacgct agatgggttt gcaccaacaa
    32761 ccgcaattct tgacgagtgg cacgaagcaa aaactcgtaa aacgtacaac gtcattaagt
    32821 caggaatgac ccaacaaaag aacggcttat tgtgtgttat tagtaccgct gggcttgatt
    32881 taaacgttcc tatgtacgag gaatacttat tgttagatcg tgtgctaaaa ggcgaagaac
    32941 aagccgacag gtactttata gcgatatggg aattggacga tcccgaagaa attcacgatc
    33001 aagagaaatg gatcaaagcc aatccgattt ttgaaagtga agaaatcaaa aaagtaatga
    33061 ttccaaccat tcaagatgat gtgaaccttg ctttgaaaca aaataacctt aattctgtat
    33121 tggtgaaaaa cttcaattta tggagacaag cgagtgagga cagctatatg attgctgaag
    33181 actggcaggc aaccgaagta gaaccacaag atattacagg caagcccgtt tatatcggag
    33241 tggatttatc taaaacagat gatttaacta gcgtttcatg gatcgtaccg ctagataacg
    33301 gcgaacttta ttgtgattca catagctttg tagccaccaa atatgggctt caagacaaag
    33361 aaaagcgcga cggtttgcct tatcgagaac tagaaaaagc tggtgagtgt tccattactc
    33421 aattagaaag tggaatcgtg gactatgacc aagtatttca gtttattcaa gatttgattc
    33481 aagaaaatga tttggaatgt atggggattt gttatgaccc gtataacgct aattcgctta
    33541 tcagtaaagc tgaaaaagcc aactacccaa tgttagaagt aagacaagga acgattactc
    33601 taaacgttcc gacccgaact tttagagaac aagtttatga aggcaacgtt attcacaata
    33661 aaaatacgat tctcacccat gcagtgaaca acgctatttt aaaaacggat aacaacggca
    33721 ttcagattaa taaatcaaag aacagtaaca aaattgatcc aatagctgca ttaatcaatg
    33781 cctatgtgtt tgcaatggat tacttcacca caacggaagg agcgaaagca gacaatgaat
    33841 tttatacaag tgaagaattt tctttctaat tacattcata ccgttctttt acttcttgga
    33901 ttggtgtgtg tgttggttgc tatcacctta ctaacaaatg tctactatgg cttgttagcg
    33961 ctgggcatag tgcttattgg ggtagcggtc atgctaaata cagaacagaa gggaggttaa
    34021 aagatggcat tttttaaagc gagacaaaat acaacgggag atcctttcct acaacatgtg
    34081 gtatcaatcc aatcggatga ttacaccacc agctttacaa gcgttcgtgc attaagaaat
    34141 agtgatgtgt ttgcagccgt tcgaattatt gctagtgata ttgcttcaag tccgattcaa
    34201 ttggtcaaaa acaatatgcc acaagctgat aatgaactgg tgaagttact aaacgagaaa
    34261 cctaattcag aaatggatgg ttggcattta aagtttgctt tggcggtcaa tatgttgcta
    34321 aacggtaata gctttgcaga gattaaacgc aacggtgaaa aggtagaaga aattcactta
    34381 ttacctaact caagtgtaac ggttactcaa ttagataatg gtacgttgtc ttatcagatt
    34441 ggtgataaaa aaagacgtgt gaagtctagc gatattttgc acttcaaata tttcacccaa
    34501 gatggtttaa caggattacc accactttat gcgttacgtg atgaactgaa aattcaacaa
    34561 gctggcaacc gcacattgca caatttcttt actcgtggtg tcagtgggtc aggtattttg
    34621 aaggttcata agtcagattt agacggatca gcaaagaacg caattcgtga aaagtttgaa
    34681 gaagccaacg gttcaagtag tggagataat gctcttagaa cgatcattct tgatgaaaca
    34741 atggactaca aaacattaga aataaacacc gatgttttga agcttatcaa ttccaatgat
    34801 tggaacacaa aacaaattgc caaggcgttt ggtgtaccga ttgagcgttt aggcgttgaa
    34861 aatgaacatt ctagcaccgt tcaaagtaat ctccagtata tccaaagtac gctgattcat
    34921 tactttaatg tgtttgtgag tgaattagat acgaaacttg aaaccaatat ccgctttaat
    34981 tccgatcagt tactagagac tgatcctgaa acaaaagtaa aaaatgtatt ggatcaggtc
    35041 aaagggtcac ttctcacgat taatgagggg cggtcgaaaa tggggctacc ccccatggat
    35101 ggtggcgatc gtttactagc aagtttgaac tttacgtatt tagatacgtt ggagaaatat
    35161 caattaaaag aacaggaagg agttacacca gttgaataat gaagaagaaa aggaaaaacg
    35221 gctgacagaa gaagctgagc taacagccga ttctcccaaa gtagagaaag aaaatgaaaa
    35281 acaaccaaca gacggcaaaa ctatttcagg ctatgcgttg aaattcgggc aaccgtcaaa
    35341 agatttaggc ggctttgtag aagtgattac acccgaagca ttaaaagagg tggatttatc
    35401 aaatgtgttc ttattatatg gacacgatta ttcaaagcca ctagctagtg tcaaagcagg
    35461 tacgttgaaa ttgaatgtcg atgatacagg attacatttt gaagcagagc taaccgacac
    35521 gacctattca aatgatgtgt atgagaatat ctcaaaaggg gttgttgatt ctatgtcctt
    35581 tgggttcgtg cttggattag attcattcga caaaaaagaa gatggcacaa ttgaacgatc
    35641 aatagataaa atcaaagcac ttaatgaaat tagtgtcgtg accgttcccg cttatgattc
    35701 atcaaatgtc caagtaaata agcgttctta cgaatcgttt atgagtaaca accaagcaaa
    35761 acaaacaaac aatagcttag aatccacttc taaagcacaa aaggagagta ataacatgga
    35821 aaaaacttta atcgataacg aaaaaacaga attgcgtggg tatgaagaat atatccgttc
    35881 acaaggcgaa gtgcgtgatg gagtcactac tgtaaatgca gcggcagttg ttcccgaaga
    35941 agtaatcggt gaagtctttg atttgaaacg ttcaaattat aacttagctc aatatgcaac
    36001 agtaaaaaca gtatcaaatg gacaaggtaa atatccagta gcaactaacc aacaagcagt
    36061 gttagcaaca aaagctgaac ttgctgaaat tggtgatatt gacgctgaaa tgtttacttc
    36121 agttgattat aaagtagaaa ctcgtgctgg taagattgcc ttatcaaatg aggttgtgga
    36181 agattcagca gtgaatattg tacaagaggt caaagatcag ttagcgaaat tggtagaaaa
    36241 cactgacaat aagcatatca tggatttatt aaaaacattc actaagaaaa cggctgctac
    36301 gttggacgat ttgaaacaac tatacaatgt ggcattagac ccagcattaa ataaaatggt
    36361 aattctaaac caaagcggat attaccacct agatacattg aaagattcag atggacgtta
    36421 cattttacaa cccgatgtga cagcacctag tggtaaatca ttattcggta tgccagtagt
    36481 attgattgca gatacattgt ttgccaatcc taaagctgga acattcccta tgattatggg
    36541 ggatattgca caatctatct ttgttgctcg tagaaatcaa gtaacgactc aatgggaaaa
    36601 attcgattac tactcacaag gacttgcagt gatcgttcgc aacgattata agaaaattga
    36661 tcaaaatgct tcagtgtata ttgagtttac gccagttgta acaccaaaag tatagaaaaa
    36721 tattgggcgg cggtttatcc cactgccttt ttttcattag gagagataaa atggtaattt
    36781 tggatagcat aaaaaaaagt atgcggatcg atcacactat tgatgatgac tttattcaac
    36841 aattgattga tacagcaggc gaatatataa aaagtgctat tgatagtagc gcaacggata
    36901 aagatatgga taattatcag caatttgatt tggcggtgtc attacttact caacattggt
    36961 atttgaaccg tcaagaagcc agcagcgaac ggataccagt aacggtacaa gcattagtac
    37021 aacaaatgag aggtgcttat tatgccaatc attaagaatg taaatgaatt gactgagaga
    37081 gtcaagttta aaaagacaaa acgggtaaaa gatgaagatg ggcagatggt ggatagtgaa
    37141 gaaactgtat ttgaatgttg ggctaatgtg cgttcacaaa tgctaaaaga cgtgcttgct
    37201 agtgtaggta ccattcttga aggaacgttg acgtttatta ttcgatacga tcaagattat
    37261 gaactaacaa acgatatgaa agtagcttgg aaaaacaaga gttataaaat tatttcaatc
    37321 aatgaaggaa cggcgtttaa agattataca acgatcatag ctaaacagat ttcttagaat
    37381 gattacactt gtaaacgttg tgggtattta gtataattaa ggtagtaaat gaagggttag
    37441 ctgcctgaat tttgcaagac ttagctagtc gaaatctatt gttggactga aaattgtagg
    37501 tgtggttgca aacatgctgg actaagaaag atgatagatg atgaacgttc aattttaata
    37561 caggcatgct aggactgatt ggatggtttt ggcaagctga ttgtgtagaa atcaaagaac
    37621 acgtctgttg tggacgtgtt ttttttgtta taattcaaaa gaggtgattt aatggacaaa
    37681 gcagagttta caaattttta ttggaagtat tatctacatt tagaaaatga gtttattaat
    37741 accacagatt ttgtgatgct cgatgagaat aatttcaaaa cattctctat tgaatatcaa
    37801 aaactactac tagcgattgg ttcggagtgt gagattattt tcaaagaact ttgcggattt
    37861 gatagcagta gcaataaaag cataactgat tttaaagctg ttattaaatc tagcgaatta
    37921 atgtgtttgg ataatggtgt tagggttatg aattctttaa cattgacttc actaaaacct
    37981 tttggagatg ggtggcctga agatacacca gattggtgga aaatatataa cagagtaaaa
    38041 catggtcgtt cttcaaatta taaggaagca aatttggaaa atgtgttgta tgctttagca
    38101 agtctatatt tattagagga atatttgcat aagaaagtaa tactagaagg tgaagtagac
    38161 tttataagtc cagagtcaaa tctatttacg ttatcttggg aaagaaaaca ttcgtcgatg
    38221 caaaaagtga cgtttgaaaa agtggattca aattatgtag ctccactcat tgattttaaa
    38281 gaacaagaag aaatttaaaa acccccacgg cttattgaag tttttgtatg ccatattgta
    38341 tgccatttgg tttaaatcgc ttgaaaaata gtgatagata actctaattc atttagtcat
    38401 aaacgctgtt ttgataacct ttatagttct ttgatatggg tttctcattc atggcggaag
    38461 aagaagaata gaacaaaaga atgaattaat aagatataaa aaaagggatt gtgacaaacg
    38521 tcttgtcaca atcccttttt ccaaataaac gatgttcaat cagctgcccc tcggcattcg
    38581 ctcgaaaaac ggcgaaatat gaagaactat tttgccattt gtttctctaa taatcaggtc
    38641 aaaacgttga cttcacagtc tcttccttaa acggtgttct cctgccaact cctcggtcac
    38701 aagtcacaat attcaacaaa catgagaagc tgttttttga aattgttcct tgtgactcgg
    38761 agctgaacgg caggctcacg acctctttct taaacggtgt tcaaaagctt gcatctgcgg
    38821 taataagttc aaccaaacga aaattatgag gagctatttt tgtttggctg ttcttattac
    38881 ttgatgcagg acagcttttt cacaacctct tttcgtttag cactaaattt ccttaaaaag
    38941 tagaaaagct ttcgaagcct cgtttgtttc ttttactgta taataaaaga aaagagtaac
    39001 aatcgctaaa agatataaac aagagaaagg aaatacaaga tgattggtct gatttatttt
    39061 gtggtgatcg ttctggcgaa cacagttggc gctatttccg ggatgggtgg aggcgtgttg
    39121 atcaaaccga ttcttgatct tatcggcgcc cattctgttg cagggatttc tttttactca
    39181 acagttgcgg tattcacgat gtccattgtt tcgactgtgc gacaagtatc aagcgggaaa
    39241 tcactgaatt ggcagattgt cggttgggta tctagtggag ctgttgttgg aggagtagtc
    39301 ggcaacattg tattcgaagt ttttttacag ttatttgaaa acgagaagca tgtgcagctg
    39361 atccaaattt ttttgacggt attgaccttg gtttttgcgt tcttctacac caagcatcat
    39421 cagccaaaat ttcatttgac gtcatggact tggtacctta tatgtggggg ggttctgggc
    39481 tttttagcta gttttttagg tattggtggt ggaccagtca atgtttcttt gttgatgctg
    39541 atgtttgcat tgccaataaa agaagcaaca ctatattctc tatctacgat tttcttttct
    39601 caactggcaa aattggtaac gattgcttta acaagtagct ttatgcgctt tgatttatct
    39661 atgttgtttt atgttatccc cgctgctgtt attggaggtt tgtggggagc aagatttagc
    39721 agggttttgt ctccaaaaaa agtgaccttt atttttcaag taattgtcct cgtggttctt
    39781 ttgatcaatc tttacaatgc tttcgtcatc ttacaaacat taggagaatc tagactaacg
    39841 caagtcaaaa tcactgaaac atattgttct aaatgaaaaa aggagtatac tgtgataaaa
    39901 aaattcattt aaaaaaataa aaattggctg gtgatttgga tgttgcggga aatgcgttta
    39961 ggagatgcag aagcactacg agaaatcaat gcggaacaat taggatatga tgtaccatta
    40021 accgttacgg tgagacaaat gaagaaactg ttgaaggagc cagaaaaaaa ttttttcttg
    40081 gtctatgaag atcctacttc taaaaaagca gctggctatg tacaagcgga gtcctatgaa
    40141 tcgattttca gcgatccgat gttcaatatt atggcacttg ctgtgtctaa acatgctgaa
    40201 aaaagaggaa tcggaaaggc cttgatgact ggcgtagaag aagcgaaaag acgaggaata
    40261 tcagcaataa gacttaattc tgcagaatac agagtagaag cacaccgatt ttatgaacgc
    40321 atcggttatc attccgataa aatgcaaaaa agatttttga aaatcctgta aatttttatc
    40381 aaaaaatacc tggtcatcaa gccgatactt gatgatcagg tatttttttt gactaagaaa
    40441 gtaattgcat caagaaacct gagatgatca aaacgatggc accaccaagc cgattgccca
    40501 tttgagcaaa agcaataagt tccatccgat tagctgcaga taagacagct acatttcctg
    40561 taccacccat cgagttgttg cataagccag cagtaatagt tgattcgatt ggatataaac
    40621 caaacaaacg accgacaaat cctgaaacta ctgaaatcgc gataacactt gtcaaacata
    40681 agacaacaaa ttgccatgtc aaagcagaag ccaatacatt tagattcaat aacgcaatac
    40741 cgataccggc aagaacagct gctgtcaagt ttttgacaat caagttgttg aacatgatag
    40801 ctgcatcttc gtaatattct ggtaataggt ttgcgatttt acagataaca acgataatga
    40861 tcatgaatgc gtaagcatgg actttaggaa caaaatagtt acagatttgt cctaagatga
    40921 agaaagtcaa tgataccatc aaaccgacac ctaattgtgg aatggaaggt tttacatttc
    40981 taggttttcc atcgccaagg tcgccttttt ccattaattt accattaccg ttgaattttg
    41041 gcattgattg tcccgcacga gcaactaacg ctgcaccaat gatagctagg acatttgtca
    41101 ttgctgtcgc aggaatcagg cgggaaatga tagaagaagc gtccgtacct aaaacatttg
    41161 cataaatcgt tgataaaggt acagctccgg ctccaacacc accagccatt gcgggcaatg
    41221 agatatataa aacggaattt gcaaaaccat ttccgataag catacctacg agtcctacgg
    41281 caaaaaagga gacgaccatt gaaataagtg caacgggaat aaaacgaaca gaagctttca
    41341 tcaatagatt acggttcata ccaaggatgc taccagtaat caaggcagca atataaaagt
    41401 ctaaaaagcc catattgctg acaaagtttt ctgtggattt tactacatac tcaggtaaga
    41461 tcccaaatgt agcaatcgcg gcagaggcaa agatcgcaaa aacggagcca ccacctaaat
    41521 aacttttgac gatagggatt tttgttccga taaaatgaaa taaattaccg aaaatgacca
    41581 aaacagcgat tgggccaatc atattcgtcg gtaattttcc taaagccatt gcaatgatca
    41641 ggacaaggac catgatcaaa taaaggggca aacttacgcc ggtaattttt gttgaccaaa
    41701 acccttcttt tttcttttcc tcaactgcag ttgcggttgt tgttttttgt gccattttta
    41761 cagatccttt cttattctgt atcgtatgaa aaaagccaaa gagaagcggc aaaagagcag
    41821 tagttggctg gctgctgttt tgccgcagtc gcttttttct ttttagacgt catacaaagt
    41881 ttctgtatgt tcttttacta taacaatctt aatcttctaa agatttatat tcaggaaccc
    41941 atttagcaga ttcaacagct tctttgatat ccgtgatttc ttcacgattc aatttttgtg
    42001 caacaactga ttttgcgaca gtttcagcaa tcgtttgaga gaattcagtg atttttgcta
    42061 ctggaggcaa gatagctgct cctggttttg ttacatcaac gattccgcct aatgctcgac
    42121 tagcttgaga aatgatttca gcatttactc gtgtagctgt tgaagcaatc aaaccaagcc
    42181 ctaaacctgg gtacatcaac gcattatttg cttgaccgat ttggtaagtt acaccgttat
    42241 attccacatc agcagcaggg atacctgttc caactaaagc ttttccgtcc gtccattcaa
    42301 tcaaatcttt tgcttttgct tctgccaatt ttgttggatt agacaaaggc atgatgattg
    42361 gacgtggggt atgagccgcc atttctttca cgatttcttc agtgaatgct ccaggttggg
    42421 tagaagtacc gatcatgatc gttggatgga ttgcttttac aactgcttct aagtttgtca
    42481 attcatcagc attagtgaat tctgagcgtt tacgagcgaa tgggatttga ccaggagtca
    42541 atccttttgt atcttcaaac aataaaccct gtttatctac ttgatagaaa tgttggcgtg
    42601 cttcttcttc aggtacacct tgacggatca tttcatctaa aaggatgttt gcgataccta
    42661 caccagcagt accagcacca aatgtcaaga tcgtttgatc tttcaattgc tcacctgata
    42721 tattcaatcc acctaataca ccagctagta caacgattcc tgtgccttgg atatcatcat
    42781 tgaaagtagt gattttatct tcgtattttt ctaagatcgt tgcagcattt cctcgtccaa
    42841 aatcttccca atgtaataac aattctggga ataattcagt agctgattga acgaattgat
    42901 caatgaattc gtagtaagtt tcgccttcta cacgagcatg acgattccct aagtataatg
    42961 gattattcaa taattcttcg ttattagtac cagcgtcgat tgatacaggc aagacttgtg
    43021 caggattgat accagccgca gcagtgtaga ccatcaattt accgatagcg atgtcaacac
    43081 cgttcacacc ccagtcaccc atacctaaga ttccttctgc atctgtcaca acgatcaaac
    43141 gaatgtcgcg tccatctgct gcgtttttca aacttgcttt gatattttct ggttcgtcga
    43201 ttgaaagaaa tgcagcatct tgtggtttca agaaaatttc gttgtattgt tcgattgaat
    43261 ccgcaacgac aggatcataa acgacaggca tgaattcaac taaatgttcg cccatcaatt
    43321 tataaaacaa tgtccggttc gtattgaata gattcattaa gaagatacgt ttttccaaat
    43381 cggtctgttt gcttaagtat tgaccgtaag cttggacaga ttgttgttcc aatgtttgga
    43441 cagtacttgg aagcatacca gtgattccgt attttgcccg ttcttctttt gtaaaagctg
    43501 ttcctttatt tagaaaagga ttatttaata agtttagccc tgtaagcatg aatgattcct
    43561 cctaagtttt aatacaaacg aagtatagga gtcagatgag gatatagtca aaaagagcag
    43621 gtataataaa ttttatttat atgagtaata gagtcaaaag aaatcaaaat agcggtattt
    43681 gttcttttta tatcaacaat ccttgcttga ttactatgcg tatctcttat atgctaaaga
    43741 aagaaaagta gaagaataaa ggatatgtta gatagataaa tagaatctct gaagaataaa
    43801 tcacgtgatc gtgacgataa aatacactta cgtcaggtga taaaaaatac ataagtagaa
    43861 aactaagagg aggaacagaa aatgaaagaa gttcaattga tcagaaaaag cgaactatca
    43921 gaaggtggtt gcaatgcatg cggcgtagta gaagcaacaa gctatacatt aaagcttggt
    43981 gccaacaaag cgataatctc tgagttgaca gtaggcggtc ttgtggacag tctggcgctt
    44041 gctgaaggtt ttattggaga agatatttat gagatgtttt cagaaatccg tcagttaaaa
    44101 aaaggggaaa actgcataga ggttcatcat gagagcccga atgtgagatt caaacgtggt
    44161 gataatgaga tgatcttcaa taatcatgtg tccgatcaca ccgagctata tggaatcgtg
    44221 aatcaaatac taactgagct ttttggattg ggaccttatg cgttcaagga agaaaacggt
    44281 aatccaaaat taaacgaaga atggcaagaa acgattgaaa tacaaagaaa caacccgcac
    44341 ttatttcaat aggcatccac agtatttcgt aaaagggact ttgacacgac ttttgtcaaa
    44401 gtcccatcgt tttttatcta tttgtgttct aaacatttct ttcagagtag taacaactgg
    44461 aatgactgaa atgtaaacag attatttgcc tgtatcaata cggaagagta atataaagtg
    44521 tgtaaacgct atcatattat tcattcaatt ttttatagag ggaaggcatt gaaatggaaa
    44581 aaacgtatca agcaggtaca aatgaaggaa tagtggattt tatcaatatg gaagatttag
    44641 aaatagccgc ttcacaagtg attccagctg gtggttatgg ttacatcagc agtggtgctg
    44701 gcgacttatt tacttatcaa gaaaatgaac gggcattcaa tcatcagctg attattcctc
    44761 atgtattaaa agatgtagaa ttacccgata caactacaca ttttgacaaa gaaacgctga
    44821 ctgcaccaat catcatggca ccagttgctg ctcatggctt agctcatgtg aaggcggaga
    44881 aagcttctgc aaaaggtgta gcagattttg ggacgatcta tacagcaagt tcttacgctt
    44941 cttgtacatt agaagagata agagaagcag gcggggagaa agcaccgcaa tggtttcaat
    45001 tttatatgag caaagatgat gggatcaatt tggatattct tgaagtggcc aagcgtaatg
    45061 gagcaaaagc aatcgtattg acagcggatg caacagttgg cggaaaccgt gaaacagacc
    45121 gcagaaatgg gtttaccttc ccactgccaa tgccgatcgt tcaggcttat caatcaggag
    45181 taggccaaac gatggatgct gtgtacaaat cttccaaaca gaagcttagt ccaaaagatg
    45241 tagaatttat tgctgctcat tctgatttgc cagtctatgt gaaaggtgtc cagtcagaag
    45301 aagatgtcta tcgttcatta gaatctggtg ctggcggaat ctgggtatct aatcatggtg
    45361 gccgtcaact agatggtggt ccagcagcgt ttgattcgtt gcaatatgtg gcagaagcag
    45421 tagataaacg tgtaccaatc gtgtttgaca gcggtgtacg acgcggacag catgttttca
    45481 aagcaatcgc atcaggtgca gatcttgttg ctattggcag acccgttatt tatggattgt
    45541 ctttaggagg aagtacagga gtccatcaag tgtttgattt ctttaaaaca gaattggaaa
    45601 tggtcatgca attagctgga acacaaacag tagaagatat caaaaaaata aaattgcgtg
    45661 agaatcgttt tatttaagaa acaaatgacg aaaaaaatag gagtgcggca gatgatgccg
    45721 tactcctatt tttatagttc aaggatgtca ctaatagaat aatttgaaac gaaaaaataa
    45781 aattgaaata ttttttcata cttaataaaa aaggaagaat attcatgtaa acgattttat
    45841 tgtttttttc tcttttatac aaaaagggtc ataatttcaa gtaagcggat gaattcatga
    45901 ggattttttg catactgtct ctactatact taggtcacta agaaaaagga gtgatttatt
    45961 atgaaaaaga acatgattgg gacattatta ctttcaacac ttcttctagg cggcttggca
    46021 acaccagctt ttgctgaggg tcaagcgact tcaaaaggag atattacctt tactgaacca
    46081 acaaataccg tggagcctct taaccctact gatccttcaa aaccagtaga gccagcagat
    46141 ccggaaaatc cagcaactgg ccagacaggt cctttaacac tggatgtagt tcccgaattg
    46201 ccatttggta cacacgagat tgaaagcgga acaaaaacgt atcaagtaga cgcttcaaaa
    46261 aacgatacac cttatctcca agtatcagat cgtcgaggtg taggtgcaga tggccaagca
    46321 caagggtgga acgtgacagt ttcggtttca gattttgtaa acggttcaca agttttgcaa
    46381 ggcgctgaat tagattttgg aacatccact gttaaaagta cgtcagataa cgaatccact
    46441 ggaccaacta gtcaaactgt aactgggttg agtaaagcaa gtgcagcgac accaatattt
    46501 acagcagcaa aagatcaagg tctgggaaca tggctttctg tttatgatcc agcaaatatc
    46561 acactgaaag tgccgaaagc agcagctgga acattcacgg ctgatttgac ttggaacctc
    46621 gtggctggcc cagtagcata gagtatgaaa taaattcaac aaactgcggt gaaaatacta
    46681 taggtgaagg attttttacc gcagtttttg ttgaaaggat gtggtggaca atgaatcaaa
    46741 gtaaacgact gtttgtttcg tttttttcta ttttaagtct atttttcatt tttccgtcaa
    46801 tcagccaagc aaaggattca gcaggagact ttggaatcaa accggtattt ccagagaacc
    46861 aaatagataa agcaatcggt tattttgatt tattagtggc accggaacaa aatcagacac
    46921 tagaagtaat tatcagcaat tcttcagatg aagaacgaac atttgaggtc tctgtcaatc
    46981 cggcggtaac gagtgatggt ggaacgatcg attatagtca aaaaaaccca acgttagatg
    47041 agacattgcc gtttgatgtc cgtgacgttt tgctcattgc gaaaaaggaa atcaatgtat
    47101 cagcacatgc agaaacaact gtacctatag aagtgaaaat cccagcaaaa agctttaagg
    47161 gacgtgtatt agcgggtatt catgtttcac ctaaagaaga agctgaaaca gaaaatgcta
    47221 cagaaggagc acaaatcaaa aatcgtattg cttataatct cgcagtcgtt ttacaagaaa
    47281 gtcaagagac gatagaaccg gatctgaaac ttttatcagg agatttggac gaggtcaacg
    47341 caaagccaac tgttcagctt cgtttccaaa atcctcagcc taggattatc agcaatttga
    47401 tatttacgag caagatcttt tatgaaaatc agctttatat tgaaaatacg tctaatgcat
    47461 ttttagttgc accaaattcg aattttcact tgaatcttga tttagctggt gataaagcaa
    47521 aagccggcga ttaccgcgca gaaatcatag caaaatcagg cgacagtaat gaatggcgat
    47581 ttacacaaaa ctttacgatc aaaaaagaaa aagcacaaaa agtaaatgaa aattccgttt
    47641 ttgcagtaca agaacaatct ttcccatggc tttatgcagg attggttgct gcagttctag
    47701 tgttatggtt gattttgctg tttttgtggg tccacaagaa aaagaaaaaa ggagaaacaa
    47761 catgatgagg tgatttcgtt gaaaacatcg atcttattgg gagggattat tgcaagtttg
    47821 ctgttcttca gctggcaaga gccagtccaa gcagatacca aaagttcagg tacgattaca
    47881 ttttatgatc taagtccaat tccgcatcca agtggttcag tcaatggcgc atcaaaacag
    47941 cagacacagc taccgattta taaagaatct aatcaacatg gcgtattccc ggagacgaat
    48001 gaacaaaaga tccgttttct ttatttgatc attggtatag aattatggct tcttgcgtgg
    48061 ttgttgttat ggagaagaaa ggaaaaggag gatgaacatg ttgctacaaa ataaattttc
    48121 attttttcgc tccttctttt ttacttcgct ttttgtttta agtatattta tcttttcacc
    48181 ttcggcagaa gctgttggag caacgcctcc tgccatttct tatgatccat acacgtatga
    48241 aaatatcgta cctccaaatg gtggacgtag tgcagtcaat ggcgggaagc ttgtccaact
    48301 aactggggct aaccaattgc ctaaattgac tgctacttat ttgaacgatg gttcaaatga
    48361 tccttggaaa ggtggcacgg tccgttttca ttgggaaagc catgtgaaac taacaaatgt
    48421 acttgatttg gtgaatatca ctgcacaagg atttgcattt gtggcgatca tcaatttgcc
    48481 ggaacatgta tcaagcagtg atgtattaaa agcaattgac tggaacaacg cctacttgta
    48541 tattgccggt gcccccatca aggttactag cggaagttta ttatcagcag gaagccatac
    48601 cttgcgtttt gggatggggt cgtgggatac aaatactcta ttgacagata ttattgctat
    48661 tattacgcga ggcgaattca acgtggacga tatcccgctt caatttgatg ttacagctaa
    48721 tgtcgcgaag atgacagaaa acggagataa atatgatacg agtccaaaca aaattttgac
    48781 caagggtaaa ttacaacctt ctatgtctaa aaaggccgtt ttttctgttg atttttatga
    48841 ttcgaataat attatccaag atcgtatgtt caattcgatt ttagctccag ctgcaggtag
    48901 attttatcct aaattaactg gcggaaactt gaatccagga gcatatgcat tttctgcaag
    48961 tgcagagata aatacgtgga atagttacat ctcaccaagt gattcaacag gaaaatataa
    49021 tacatttgct gataccacag aatacgtgga tggaaataac cagaaccttc caggctctac
    49081 caatagtcgg acattgacga tggggctgac tgaaggacgt tttgatcagt tgccatttag
    49141 tcgctttgat cgcgtagtca actattttac aggaaaaaac gttacacaag gagcaatact
    49201 gactcatact ccagcaagca caagtggatt gaatcagaca gccaatatcg tgtacagtgg
    49261 gaaagatgcg caaggcggat tgctttctcc tgttgcttta aaggtattgg agcagtacaa
    49321 gctagacgga acggttttac caacaaactt aacagccgat agtcagtggg gaaagctcct
    49381 ttcttctgca tcgtaccaag tcacggccaa ttggtcagca actgaactaa aaaagggttc
    49441 gatccattac aggctttatt cgaaaggaac ccaaaaatta gcgacaggat ggacagatca
    49501 acttttccaa acgatccaat caaataacgg gggtgtaaat acagctactt ctacactacc
    49561 tgcattggct tcagggcaat attattttga ttatcgcttg gtggacgatg tacttactaa
    49621 catgtatccg tcttttgcgt acaaatggca gtctgaacaa gctggaatca ccactcttcc
    49681 tcaaataaca gtggctaatt ttccatctgt gagtgcagat agtcagctaa aaaaccttag
    49741 cagacaacct gagacaggag atcccctgtc tgctctatca ggagatacga ttcaagaaaa
    49801 tgtgacattc acgttaacaa aatctgggga tgcgataacc gacaaaaaaa tcacgatctc
    49861 acttcctgaa aatactactt atatcaatgg ttccttaaaa ttaaatggta caactatcgc
    49921 agataccggc attcaacaag gagtttctgt cccagcagat ctattgagca aaataggaga
    49981 taccattcat cttacttaca actatcaatt aaatactgta gatacttctt tacaatccgt
    50041 cagcatactg acaaaggcag ctgtactttc cagcaatatt acattagcag atggtgctaa
    50101 gcttcctaac ccagttgtcc aaacatctgc taaaacaatt cttgtcccca aacaagaatt
    50161 aacattagtt aatgtaccag atgattttac atttggtaac gatctcccca aaccgttgaa
    50221 gacaagttat tatgaatcaa aaggcgattt ttcttttgat gtgcgggata ctcggcttcc
    50281 aagcacgtct ccttggcaac tgacaggtac attgacttct ttatttaaga atgatcaagg
    50341 tcaagaatta agcggaacaa aattatactt caatcattct ggatcaaagc aattgattca
    50401 gcaaggtcaa aacacgttga tttatgagag tgatggaaca gctaaaggag aggttctcgt
    50461 tgactttccc gataccgatg gtttactctt agaggtaaat agcagtacga atgctcaacc
    50521 tggcgcaacg tatcaaggca tggtcacatg ggaactgaca gctgggccaa ccagctaagt
    50581 gattagaatt tgtatggatc tgtgataact gtcacagatc ctctttttca aggatgagaa
    50641 ggaaacttct gctgttaaaa tcagaaatgc ttgaaggagt ggacttggaa cttttcagtt
    50701 tgcttgaaaa aaatgaacaa cggcaaatca aacttttcca acttttatta gaggaaaaag
    50761 gagaggtttc cgttaataac gtacttaccc gcttatcagt tgatcgggct accttgaaag
    50821 aagatctgtt ttatttacaa gctaacttgg aaaatatcaa acaattggaa attgttatcc
    50881 aagaagggaa tattttatta atcagacatg gaaatatagc agcagtcgat gtttattatc
    50941 attgttactt gcgctattct acaaaatatc agatacttct ttatctgttg gaaaatggct
    51001 catttgatcg gcagaaactt gtcatggaac ttaatatcag ttcggctact ttgtctcgca
    51061 gagtgaagga attaaatgat tctttaaatg agtttcatct gaaaataaaa agcgggcggc
    51121 tgaccggtcc agaaagtcaa attcgttatt tttatttcca attattttgg tttggacgtc
    51181 cctatcttga aaacaaaaaa gaatacagtg atttatcagg aaacactttt ttagatctgc
    51241 tgcaaaaaga gtttagtttt gctattacag aagaaggacg attgaaattt gaaatatggc
    51301 tttccattat gaagaagcga atgcgctgta cgaaaaaatc caaaccagat tatagcaatc
    51361 ttcctgttgg attgtttcaa acgacacaat ttttattgga cctccataca tttttgaccc
    51421 gttacttctt tcaatatgcc tttccttgga atcaaaatga aacgttggtt ttttatcttt
    51481 tcatgatcag taactttaca ttagatacac ataactcgca tgtccaggag tttttgataa
    51541 gcgaaaaata caaagacgac gtagtagctc aattgaatcg aacgtttaaa caatcgatag
    51601 aagaatattt ctctttttat gtttttccag aagagatttc gcaaaagatt gcgttgacag
    51661 tgatgcaaaa tcacgctcat tttgcctatt ttagaggatg gatgaatatt tttggccaag
    51721 aaggattaaa acagcggtta actgaagtag aagataaaca attcctggtt ttatgtgaaa
    51781 gattggtcag acaaacattt gctattgttg attttactga agtagacgag gaaaatgccc
    51841 gctttgaatt atttgctcgc tatgcatcta ttttacggac gctgatcaag cagttagaat
    51901 tacgtctgca aattgcttat gatcttcctt atgaaaggct aatggcggat atcgtgaaag
    51961 aaatcatcca ggaacaattg ctgaatacga tcaaatatga tttagtcgaa tacgagaaag
    52021 aaaagcagta tgatatcatt ttgactagcc aattgaaaga gtacccgtca cagaagtccg
    52081 ctaaagtttt tgtattcact agcaacgaat ataattatga tttcccctat ttgaaccaat
    52141 ttttaaaaga atgttattta gaaaaattga atttaaggat gaataaaaca tgaaaatcgt
    52201 gatcattggt gcttcccatg aaggcatcac agctgcccta aatctaagaa aacttcaacc
    52261 gaaagcagaa gttttactga tcgatgaaga tcataaagat ggactaggct atgtttcaaa
    52321 tggaatcaac ctttatttaa aaggaaagat acgtagttta gctgaagttg cccataacat
    52381 gcggactctt caagaaagtg gggcaaaact gatcaccgaa tggagagtaa ctgaacttga
    52441 tccagacaaa catcagctca tcctcgcatc caaagaagga aaaaacgaga ccataaccta
    52501 tgataaatta atcgtagcga caggttcctc tcctgtcacc ttgtataaac aaattgaagc
    52561 tgaaaatgtc tacacctata aaaatctagt gcaatctaag caagtacttg ccgctttaaa
    52621 agaagcaaaa gaagtagtga tcttcggtgc tggttacatt ggattggaat tagcagatgc
    52681 tttgagaaat aaaggataca ttattcatct agttgattat atgccgaatg ttttatcaag
    52741 atatttcgat aaggatatga tcaacagttt tcaaaatcag ctgcaaacta agcaaataaa
    52801 tttttatcca aatgaattct tgatcgattg gaaaaaatca gaagagaaag tagtatccgt
    52861 acaacttctg tctcaaacaa taaaggcaga tatggtcatt ttttctgccc aaactcgtcc
    52921 taatacgaca ttgttaaaag aaaaagtggc tttgtatgag gatgagactg tgatagtcaa
    52981 tgaatatctg cagacaagtg atccggacat ctatgcgatt ggagatatcg tcccagtcag
    53041 ttttgacaaa aataaacggc atctcttttt acctttagtt actcgagctg tacatatggc
    53101 aagagcagta gcgctaactt tgagtggtca gccaaccgct tacgacctca gacaaaagat
    53161 aacagcagca gtcattatag actacttttt aggaactgta gggctgacag aagatgaagc
    53221 gccattcttg gaacaaaata ccggttcgtg ttcaggagaa tttgatctat ttccacaata
    53281 cgacgaagaa aacaaaacag taaatgcaaa gctgatctac catccggata cattagaaat
    53341 aattggcgga cagctgataa gccaagaatt cttattatca gatttgaacc tgcttgcgga
    53401 tatcgtgaaa cacaaaacta cgattccaca gctagcagtt gaggattttg gatttctgtc
    53461 tgaatacacc tctcgttttc actacctcaa tgagttagct tttaaagtac tagctaagag
    53521 agcaaatagc aatagagtag ataaaagact gtgagaaata tcacggtctt ttattgtgtg
    53581 gggatataat aaatattaat caatcgagaa taaaaaattc atataatgaa atagcggaag
    53641 aattttttga gcataatatt cgccaaaaat ttatcaagtg ataaaattgt tgtataaaat
    53701 gattatttca ggaggaggag ttaaatgttt ttagcattaa atgaaattat gcattcaaag
    53761 ttacgttatg cgttagttgc aggggtgatg tttttgatcg cctacctggt attttttttg
    53821 actgggttag cttatggctt agctcaagat aatcgaacag cagtcgacaa gtgggaggca
    53881 gattcgattg tgctttcgaa agatgcaaat tcaaatctag ggatgtccat gattactaag
    53941 aagatagcag aagaagtcga aggtgggaaa gtggcgtatc ttgcccaaac acctggagtc
    54001 gtgactagta aagacagtac agaagaagga aaaatcaatg ttagcttctt cggtatcgat
    54061 aaaaatcaat tcatcatgcc caatcttgtt gaaggaaaag cttttgataa cgatgatgag
    54121 gcagtaggcg atattagttt gaaagaagaa tatggtctag ccataggaga tacagttaag
    54181 ttatctggta gtgacaagac gtttaaacta actggcttta cggatcatgc caaattcaat
    54241 gtctcaccag ttctctatac aaccatcaat gcttaccaag aaatccgctt cgaaaaagaa
    54301 gatacaagtg aaaatgcacg gattaacgca atagttgtca gaggtaaaat cagtaattta
    54361 cctgaagatt tagaacagat caaaatttct aaattcatca atgaattacc tggatataat
    54421 gcgcaagtat tgacttttgg atttatgatc ggatttttga tcgttattgc ggctattgtg
    54481 atcgggattt tcatttatgt actgacgatg caaaagatta atatctttgg tgtaatgaaa
    54541 gcacaaggga tcacaggagg atttattgcg cgttctgtag tggcacaaac attcatttta
    54601 tcatttgttg gtattcttct tggtctatta ggaacagtgg gaacatccct tgttctacct
    54661 gatgcggttc cattccaaag caattggctg ttttttggcg tcattagctt gttgatgtta
    54721 gtagtagctg tgttaggcgc actattttct gttcggacaa tcgtaaaaat cgatccgttg
    54781 aaggcaattg gataagaagg aggaagaaca tgaaagcaat agaatttaaa gaagtagaaa
    54841 aaagtttcca agatggcgac caagtagtta aagcattgaa agaaacaacc atgagtatcg
    54901 ataaagggga atttgttgca atcatcggtc cttctggttc aggtaaaagt acttttttga
    54961 ctttggcagg cggattgcag acgcctagca gtggctctgt atttatcaat gatcaagaat
    55021 tcagtaatca aaaagaaagc gaacgcgtta aactacgttt taaagaaatt ggctttattt
    55081 tgcaagcttc caatctggtt ccttttctga cagtaaaaaa gcagttatta ttagtagata
    55141 agattaaaca tgaacatcgt caaaaagaag caaacgaatt atttgagcag cttggcgtgg
    55201 ataagttgat caacaagtat ccagaagaac tatctggtgg agaacggcaa agggtcgcaa
    55261 tcgctcgtgc attgtacaat gatccgtcca ttattctagc agatgaacca actgctagtt
    55321 tggattcaga aaaggcacga gaagtagtcg atatcttggc aaaagaatca aaagacaaaa
    55381 ataaagcaac gatcatggtt actcacgata cacggctgat tgacaaatgc gatagaatat
    55441 ttatgatgga agatggtgta ttgaaagaaa aagaataacc aaaaagcagg tggaaaaatg
    55501 gtccatcgat acaacaaaga tacacagcaa aaaattttga aagaagcgac tgacttgttt
    55561 atgtccaagg gatatctcgg cacttcaaca agagaaatcg cacaaaaagt tgggatcacc
    55621 cagcccaatc tctatcatta tttcggtgat aaagaaaaac tatatacggc agttttagaa
    55681 aaccacttaa aagatgttgg aaaagctttg agagaaatcg ttcaaacaag cgaaacggga
    55741 tttcaagtaa ccttgacaaa aatggcgcag tttttgattg agacacatct ggtcgatttg
    55801 tttctgatgc ttcacgatct tgaaagcaat ctttcaaagg aaacaagaga tcatcttttc
    55861 tttctatgga agaaaaatta tcgtgaacct tttgaagaaa tcttttcagc aaaccaatct
    55921 gtgctaagaa atgggatttc aaaagaaatc gcagcaaggc attttttctt agtgttagca
    55981 ccttacatca ctcggtcgaa aagacctgtg gaagaaccgc taaatgtggc gcaattgatc
    56041 gatttatatt tgaatggtgt attaggagaa gcgtaaagct gttatatagg gagaaaatag
    56101 ttaggtgaag aagcaagata tgacgaagat cgttatatct tgcttcttta tataggtagt
    56161 ttttcataaa tacaaatttg ttattctctc atgattatca gggtatattt ttatttatta
    56221 tgtcggtgtt ccaggaagtg ttttaattta ataacgaaga aattaattgt ttgtagaatg
    56281 aaggaaacta tttattgggt agatgaatca ccacgaatag tcaaaaggag atttcaggat
    56341 atgccaaaaa tgaaaaaaat cattttagca gtaatgttat tttctacggt tatatctttt
    56401 acagcccctt ttttgcttac ttaagctgat tttaagtttt ggcccttagt ctggatctac
    56461 gaggtgagag tcaagaatga aaaaaataat tggagctttt gtatttgcaa tcattatcat
    56521 tattatctgt tttcaaatag atgtttttgg tcaaaaaaag atagttggac aaacggtcag
    56581 cagtacgaac agtacggtta caaagacaaa ggaatcattt gatgatacag aacaagagac
    56641 attaatcaaa atcggaaaaa atgaccgtac actatataca gatgactcgc taactacccc
    56701 acttgcaact ataaacggcg gagagctggc ggagtttctt tctgaaacaa aaaatgctta
    56761 tcagatcaag acaaatgatg gctatactgg atatttagcc ctcgaagatg gaaccaaagt
    56821 cacaaagaat atacaaacaa aaccggaaga gctatctgat gcagtgattg tattagatcc
    56881 tgggcatggc ggaaatgata ctggggcatt aagcaatgat gaacaaacag aagaaaaaga
    56941 tataacatta agtacagcca taaaagtaaa aaaggcgcta gaggatgctg gagcaacggt
    57001 ttatttgacc catactacag atgaattagt acagcttgga gatatatgcg actacagtga
    57061 agaaaaaaag gcagatgtct ttatcagttt acacgcagac tctacggaat atgcgaatga
    57121 agcaactgga atcacgacat attattacta tggacaagag gaaacattag cacagacaat
    57181 tgctgactcc ttcactgatc tgcctctgga ttcaagagga atctccactg ggaactatca
    57241 agttcttcga gaaaatcttc aaccttctat tttaatcgag atgggttata tgaataatga
    57301 tagtgattta aaggaattag taacagacag ttatcagcag cagattgctg caagtttgac
    57361 acaagcattg accacttatt ttcagtaata aataagccca gccaaacaaa aatagctcct
    57421 catatttttc gtttggccgg gcttattatc gcagatgcaa gcttttaaac accgtttaag
    57481 gaagaggtcg tgagcctgcc gttcaactct gagtcacaag gagcaatttc aaaaaacagc
    57541 ttctcatgtt tgatgactat tgtgacttgt gaccgaggag ttggcatgag aacaccgttt
    57601 aagaaaaagg ttgtgaaatc aacgttttgt tctgagtatt agagaaacaa acggaaaaat
    57661 agttcctcat attttgccgt ttgttgagct aataccgagg atcagatgat tgaacaccgt
    57721 ttattcggaa caagggactg tgacatgact tttgtcacag ttccattatt tttttagtag
    57781 cttcaaagct ttttctgtat tggcaaaatg aagtttggca tatttttcaa gcataggcat
    57841 gccgcctttc tccaatagtt ttgccgctac ttggtcagat ttcgttccag ctcctgacgg
    57901 aagggtgatt ccagcttcag ctgatgcttt gtctaactct tccaaaaagc tggcacgact
    57961 gatgatagaa gcagcagcaa cagctaaatg atattgttct cctttagtaa cgaagtacag
    58021 tttttcttgt aattgatttt tttctaaacg cgcatatttt ttaaaatttg cttccggcgt
    58081 aaactgatcg atcaaaatcg cttctggttt ttctggctcg atttttttta gcagcaaatg
    58141 gatcgcttga ttgtgcaaag cgactttcat ccgaacggca ttgtattttg gttggatctc
    58201 attgtatttt tctggagata agacaagcag tttatagggg atggtttttt ttaagatgtc
    58261 agcaagctgg atgatttggc tatcttttaa ctcttttgaa tctctaacac caagtttacg
    58321 taattctgca agctgatcac gtttcacata agcagcacat actgttagcg gaccgaaata
    58381 gctgccattt ccgacctcat cactacctaa aacggataat tgactgatat tttttggcaa
    58441 agaagaggct gtttttgctg gcttggcttg tttatcctgt gcctcccccc atttatcagc
    58501 ttctattgct gcattcgtac cttgaaacat cacttttccg gatgtataag cggtaatggt
    58561 cgtcccgtct ttttttgcag caaagctagt ataaggaatc tgtttattta acaaataaga
    58621 gcgatagtat gctttcattt gactaagttg gtcgtttgtt gctttgataa tgacgttgct
    58681 cataaaattc tcctcgtttt tatacataaa actataccgc aactcacgac ttttcgtttt
    58741 ctaagagatt gcgagacata gcattttttt aaagtttatc atataatagt taagtatgtg
    58801 aaaaatattt gtggagaacg gtgaaagaac caacttcatg gtacactaaa taaaactttc
    58861 atcaaatagg aggaaatgat tcgtatggcg cacgaaaaaa cacgctacaa agcggtgatt
    58921 gccaaccaaa catatactat tatcggacgg gaaaccaagc atcatatgga tatcgtaacg
    58981 aagctgatca atgaacagtt agcagaattg aaacaattat caccacaaat ggataatgaa
    59041 caagcagcaa tcttgatggc tgtcaatgcg ctttcggatc aattgaagaa acaagaaaga
    59101 atattggagc tggaagaaga aacagcagaa ttaaagaaaa aaatgatcaa atttacagag
    59161 cttgaaaatc gtgtgaaacg cattgaagca atcgagaacg aagctcggga agtattgaaa
    59221 gaaaatggtc aagcagatca taccattaaa aatcatgtgg aagcacaaca gattttaaac
    59281 gagaaacgaa aaggacaaat taaacaaaaa tcttctaatt aagatccttt tatcaaaata
    59341 agaaaaattt aaaaaagtta gaagagaaag gaacgtagca tgctgagttt gctgatttta
    59401 tttttattgc taattgcgtt tttttctgga gcacgtcgcg ggtttgcttt gcaaatcata
    59461 ttcgcgattg gatatgtatt gtcatttatt gctgcccagc atttttacaa accattagca
    59521 agtcatttgg aattatacat tccataccca gctgtcaccc caacatcaaa gttagcattt
    59581 tttgatcagg tatttgcctt tcacctagat gaagcttttt atgcaggaac tgcttttctg
    59641 attattttgc taattggctg gctgcttacc cgatttgtgg gggtttttgt ccatggattg
    59701 acgtatgtgc cgatcttgag acaggtagac tggattgcag gaggcatcct gtcactaatc
    59761 atggcttacg ttactatatt tttgattttg cagttacttg cctttgttcc attagatgtg
    59821 gtccaaaatc aattcggcga gcatagtttc gctcgtttta tcgttgagag aacgccattt
    59881 cttaccaaca agatgtatga gctttgggta acgaatattt tgaattaagg aaaatagtgc
    59941 tggaaaatat ttcgtttttt gtacagagta gtttatttaa gaaggctgcg tagcgtatgt
    60001 ccggcacagc tcttttttca tagtgaaaga taaggtgata aaatgaataa ccgaatcctt
    60061 gaaacattgg aatttgaaaa agtcaaacag atggtcagac aatttgtagt gactgcacaa
    60121 ggaaaagaag aactggcaga attggtacca gtgagtgaga aacaaacgat cacaaactgg
    60181 cttcaagaaa ctgaggatgg attgaaagtg caacgtctgc gaggcgggat ccctatacct
    60241 aaaattgaaa atatccgtcc ccatatgaaa cggattgaga tcggagcaga cttgaatgga
    60301 gtggagcttg cacaagtttc cagagtattg tctacgacaa gcgaattgaa tcgtttcatt
    60361 gatgatcttt ctgacagtga aatcgaattt gcacgtttgt atatgtgggc agatcagtta
    60421 gtcacgatcc ctgttttgag ccgccgttta aaggaagcaa tcgatgaaga cggacgcgtg
    60481 actgatgacg cttctcctga gttaaagagc ataagacaga acattcggcg aagtgaacaa
    60541 gctgttcgtg aacagttaga tgggatcgtc cgtgggaaaa atgcaaagta tttaagtgat
    60601 gcgatcatca ccatgcgaaa tgaccgctac gttatcccag tcaaacaaga ataccgcggt
    60661 gtatttggcg gtgtagtcca cgatcaaagt gcttcaggtc agactttatt tattgaacca
    60721 aaacaagtgg tggatttgaa taatcgtttg cgtcaatacc aaatcgctga acgaaatgag
    60781 attcaacgta ttttaagtga actttctgct gaacttgttc cccatcgtca agaaatcatc
    60841 cacaatactt atgtgattgg taaaatggat ctgatgaatg caaaagcacg atttggtaaa
    60901 gaagtaaaag ccatcgtgcc gggaatcagt gaagacaatc atgtcgtctt gaaacaagca
    60961 agacatccac tgatcgatca ggaaaaagta gtttctaatg acatcacaat cggaaaagac
    61021 tatcaagcca tcgtcattac aggacctaac acaggtggga aaacgatcac gttgaagact
    61081 ttaggtctgt tacaactgat ggggcaagca ggattaccga ttcctgcagg tgaagaaagt
    61141 caaatcggta tttttgagga agtatttgcg gatataggag acgaacagtc gattgagcaa
    61201 agtctatcga ccttttcttc tcatatgacg aacactgtag atattttatc aaaagtcaat
    61261 gaaaaaagtc tggtattatt tgacgagctc ggtgcaggaa cagatccgca agaaggtgct
    61321 gctctagcaa tcgcaatctt agatgatctt ggtaaaaagt ctgcttatgt gatggcaaca
    61381 acgcattatc ctgaattgaa agtttatgga tataatcggg caaatacgat taatgccagc
    61441 atggagtttg atgtggatac attaagtcca acgtatcgtt tgctgattgg cgttcctgga
    61501 cgaagcaatg cgtttgaaat ctctagccga ttaggtcttg atacagaagt tattgatgaa
    61561 gcgaagcagt tgatgaatga tgagagccaa gatttaaacg aaatgatcac agatttagag
    61621 aatcgccgga aaatggcaga gaccgaatat ctggaaatgc gtcattttgt ttctgaggca
    61681 caagaacttc acgatgattt gaaagaagct tacagctatt tctttgaaga acgtgaaaaa
    61741 gaaatggaaa aagcaaagaa aaaagcaaat gaagtcgtgt cagaggcgga agaaaaagca
    61801 gaaaaaatca ttgctgatat tcgtaaaatg caacagcaga ttgggcaagg aaatgtgaaa
    61861 gagcatcagt tgatcgatgc aaagacccaa ttagcaaatc tacatcaaga agaaactttg
    61921 aagaaaaata aagtattgaa gaaggcgaaa gaacaaaaaa cgttgaaacc gggagacgaa
    61981 gtgttggtta cgacttacgg ccaaagaggg acgcttttac gtaaaaacgg gaatcagtgg
    62041 caagtagaaa tcggtatatt aaaaatgaat gtgtccgaag acgagctgac gcctgttgct
    62101 cctcaaaaag agccgacaca acgagttatc catgctgtac gatctgaatc tagtagtcac
    62161 gtgccgaatc aattggacct tcgaggaaaa cgatacgaag aagcgctaag tgaggtagat
    62221 caatatttgg attctgccat tctagctggt tatcctcaag tgactattgt ccacggaaaa
    62281 ggaacaggcg cacttcgcaa gggaattact gattatctta aaaaccatcg gagcgtcaaa
    62341 agctttgagt ttgcaccggc aaaccaaggc ggaaatggtg cgacaattgt taaatttaag
    62401 taaagcttac taaaggtaag cagatggttc gctatccaag tatagaaatg ctatactagt
    62461 catagatttt aaaaatggag gtctttgata tgtcacaagt aattacagat aagacattta
    62521 atgaagaaac agataaagga cttgtattga ttgatttttg ggcaacttgg tgtggtccat
    62581 gtcgtatgca agcaccgatt ttggatcagt tggaacaaga atatgatgaa gaagaattca
    62641 gaattgctaa aatggatgtg gatgaaaatc cagaaactcc tcaacaattc ggtatcatga
    62701 gcattccaac attaatgttg aaaaaagatg gtcaagtagt agaaaaagct gttggtgttc
    62761 attcaaaaga acaactacgt caaatgatcg atcaatattt ataaaaactt acagcaaaaa
    62821 taagctgagg gtgggactga atccagttcc gtcctcggtt tttttgcggt tgagttaagg
    62881 ttattcaaat taatattttt tgagtataat tacgttgtca ggctggattg tttttttgac
    62941 agacatgcta taataacttt aattgaatat gaaggggagt accagatgaa cgaacgaatc
    63001 aaaaacaaat tagctttact tcctgatcag cccggctgtt acttgatgaa agataaaaat
    63061 ggaacgatca tttatgtcgg caaagccaaa atattaaaaa atcgtgtccg ctcatatttt
    63121 actggtagcc ataatacgaa aacggaacgg cttgttagtg aaatcgaaga ctttgaatac
    63181 atcgttacag aatcgaatat cgaagcattg ctcttagaga tcaatctgat aaagaaaaat
    63241 gatcctaaat acaacattat gttaaaggat gataaaacct atcctttcct taagataacg
    63301 aatgaaaaat atccccgttt ggtgattact cgtaaggtac taaaagacaa agcactgtat
    63361 tttggaccat atcccgatgt cggtgcggcg aatgagacaa agaagatcct cgatcgatta
    63421 tttcctctga gaaaatgcaa acctagtcag acgaaggagc cttgtcttta ctatcatttg
    63481 ggacaatgcc tgtgtcctta ttatttcgac gtagatccga aagtctatac gaaaatcgtt
    63541 gatgaagtga aaaacttttt aaacggagga cacgaaaaaa tcgagtcaga gatccatcag
    63601 aaaatggaaa aagctgctga aaacatggag tttgaaaaag ctgcagaata ccgagaccag
    63661 atccgtgcca ttgagacaat catgactcgc caaaaaatga caaatactga tttactggat
    63721 cgagatgtat ttggttatgc gattgataaa ggatggatgt gtgtccaagt gtttttcgta
    63781 agacaaggaa agctaattga acgagatgtt tcaatgtttc ctttctataa cgaagcagaa
    63841 gaagatttct tgacttacct cggtcagttt tatcaggaaa atgagcattt cattcctaaa
    63901 gaagtcttga ttcctgatac gatcgacaag cccagtgtag aagctttgtt agctacgaaa
    63961 gtgctgcaac caaaacgagg agaaaagaaa aaattagttg aattggcgag taagaatgcc
    64021 tcagttgctt tgaatgaacg gtttgatctg attgccagaa aacaggaacg gacgatcgga
    64081 gcagttgaac gtttaggaaa cgcgatgaat atcccgaccc caatacgtat cgagtctttt
    64141 gataactcta atatcatggg gacgaatcct gtggctgcga tggtcgtatt cattgatgga
    64201 aaaccggcaa gaaaagaata tcggaaatat aaaataaaaa cagtcaacgg accggatgat
    64261 tatgcatcaa tgagagaagt catctatcgc agatattcgc gagtgattcg cgaagggttg
    64321 cctttacctg atttgatctt gatcgatgga ggaaaaggac aagtagatgt ggcaaaagat
    64381 gtcttagaaa atcaattagg aatcgatatt cctattgctg gcatggtaaa aaacgataaa
    64441 cataaaacaa atgagttgct attcggtaca gatttggctg tagtaccgct tgagcgaaat
    64501 tcaccagagt ttttcttact ccagcgaatc caagatgaag tccatcgatt cgcaatcact
    64561 ttccatcggc agctacgcag taaaaacagt tttgcttccc gattagatga aattgaaggg
    64621 ttaggaccaa aacgaaaaaa aatgctgctt aaagagttca aatcgttgaa aaatatcaca
    64681 gcagccacga cagaagaatt acaagaaatc ggactgccga aaaatgtcgc acaaaacgta
    64741 tttgataaat tacatcagga atgaggaaag ggcactttac aaagaaagta aagtgtccta
    64801 tcattttatg tacaacaaaa agagcgttta cgttttgttg gtaaataata aatcgcttac
    64861 gtctggtgtc taaggatata tgtgatttct tgaagaagga gtgaagaaat gttagaagtc
    64921 aaggatcttg ttaaaacttt tgggaactac acagctgtcg atcatgtctc tttccaaatc
    64981 cccgatggaa aaatcatggg gctaattggc caaaacgggg ctgggaagac aacgactttt
    65041 cgtttgatct tggatttttt gacacaagac caaggagaag tgttatggaa tggccatcat
    65101 cttggggaga aagattatga tgtcatcggt tatcttccgg aggaacgggg actttatccg
    65161 aaagtaacga ttcaagacca actgctttat tttgcagaac ttagagggaa atcaagaaaa
    65221 gagattgaac caaaaatcga cttttggatg gaaaaatttc aggtaaaagg gaaaaagacg
    65281 gataaagtaa aatctttgtc taaaggaaat cagcaaaaag tccagctgat cgccacgtta
    65341 atccatgaac ctaaattgat cattttggat gagccattca gcgggttaga tccggtaaat
    65401 gctgaattgc taaaagatgg aatattggaa ttgaaaaaac atggttcatg tgtcattttt
    65461 tctagccaca atatggataa tgtagaaaaa atctgcgatc atttgatcat gttgcgaaac
    65521 ggaaaaatgg ttttgaatgg aaaagtacat gagattcgtg aatcatttgg ccgtacgaag
    65581 ttattcttgg aatctggttt aagccaacag gaagtagaag aaatcgaagg cgtgaaaaaa
    65641 gtgatccttc atgaagacaa tacgttagag attaccttag aagatcctga agctgggaaa
    65701 gaaatcttta ctcgtgcgac tcagttcggc tacattccta tgtttaacca gcaaccgcca
    65761 actttggaag agattttcaa attgaaagca ggtgaaatcg atgcgtaagt tttggattat
    65821 taccaaagat gtctatttaa aaaatgttaa gtcgatttct ttcttgatca tgatattagt
    65881 cccttttatc atgctgggta tcatttatgt agcaggaaac tttgctcaac aaaatagtga
    65941 tgtcaataaa atcggcgtat tggctgataa tgagcaaatc agtcagcaaa tgagccaagt
    66001 caaaacagat gattttgagt tcaaagcagt tgattctgaa gaaaaagcac aaaaagagct
    66061 ttcggaagaa aaaatcgatg cgtatttagt agtctcaact gagaatgata agataaatgg
    66121 gatgctttat agcgaaaatt cattaggaca aagtacacaa ctactgatac aacaacaact
    66181 gactggcttg caatcgatgc ttcgagcgag tcaattaggc atttcaccag aagaagtagc
    66241 tagcttgagc caacaagcaa gtttgactcg acaaaaagtc aattttgatt caaaaggaaa
    66301 aatgacagtc ggagaagata actcgacagt ccaatacgtc gtaagttatg tagcaacgat
    66361 tgtcttgttc attattattt tgacgtatgc gcagatcatt gcacaagaga tcgcttctga
    66421 aaaaggcacg cggatcatgg aagtgatcct gtccagtacg actgcacaaa agcattttta
    66481 cggaaaatta acaggtgttt tacttgttgc attaacgcaa atggcattat acggtattat
    66541 ttttggtata ggatacagtc aattcaaaga tatggaaatg gtcaaaaata tattagacgg
    66601 tatctcattg gataacttga ttggaccatt cttgtggtat tctcttctat tcatgatctt
    66661 tggcattttg attttttctg ttttagcagc tttatgtggc tctttggtca acaaagcaga
    66721 agatacagca aaagcaattt tgccagtgac ttatctatct ctaggcggat acatgcttgg
    66781 attgatacta ggagcttcag atccaaacaa tatcgttatt cggatcactt cgtatattcc
    66841 attcttatct tcatatatta tgccgattcg cttagcaaat gagacagtcg gtatgggtgg
    66901 agcattgatt tccttgttgg tacttgtagc cgtaacattc ttattgatga tcttgtctgc
    66961 aaacatgtac aaatcgaatg tactcgttta tagcgaaggc ggattatgga gttcattgaa
    67021 acaatcgatt tcaattatga ggaatgaacg aaaaaaagca taaaacaatc ctaaaataca
    67081 gaaggaagct ggacatctat tgttcccagc ttctttttgt atagacctat tttatttatt
    67141 ttactatatt ggatatatat tcattgaaaa tagtgtataa caactttata atattgagta
    67201 taccaatttt tggaggcgtt ttaatggacc gttatttaga tttactttca gaaaaattcc
    67261 cctcaacgga ggatgtcatc acggaaatca tcaatctcga agcaattttg cagcttccaa
    67321 aaggaacaga gttatttttg agtgatatac atggcgaatt tcctgctttt gatcatattc
    67381 tgcgtatcgg ttcaggaaat cttaaagaaa agatcaaaga ccttttttct gaacaaatga
    67441 cggaaacgga tatcagccaa ttcactattt ttacggctta tccagaatat gcattaacga
    67501 ctgattggta caaagaacaa gataaatcgc agctgatcca tcatttgatc gatcttttac
    67561 ggtttacgac agtcaaatat acccggtcaa aagttcgaaa aggattgccg aaagaatata
    67621 gctatatcat tgaggaactc ttgtatatcg atgaccgtat caatggaaaa aaagactatg
    67681 taaaaaaaat cattgaacaa cttgttttga tgaaggaaga agaaaaattt ttaacgaaac
    67741 ttgcacagac gatccaaaaa tctgtcatcg atcatttgca tatcgttgga gatatttttg
    67801 accgaggaac tcaagcagcc aaagtgatgg atcgaatcat ggatttttct tctgtagata
    67861 tccaatgggg aaatcatgat attttgtgga ttggtgcgta ttgcggctct gaggcatgtt
    67921 tactgacact tttacggatt gcagcacgct ataattattt atttgaatta gagaaagaat
    67981 atgggttgaa tttacgtcca ttgttttctt ttgctcatca aacttatcag aagaatccag
    68041 tatttgcgcc taaaaacagt aaaaatctat ccgaaagaga gcaagaagaa ttagaaaaaa
    68101 tccatcaggc actttctatc cttcaattca aatcagaaca tcagttattg gatcgtcgac
    68161 ctgaatttaa aatggacgat cgtaaattgc tggaaaaaat cgactatgaa gcatcaacga
    68221 ttgatttaga aggtcagctc tatgagttga aaggaacttg ttttcaaacg atccagccag
    68281 atgatccaaa aaaattcttg gaagaggaac aaaaagtgat tgatagtttg atgtattcat
    68341 tccagcactc tttgagaatg caaaaacata tgacgttttt gatcgaaaaa gggggaatgt
    68401 atctcacgaa caatcagcat gtgctgtttc atggctgtat acctgtagac gagaagggac
    68461 agctcttagc atttgaatta gaccaatctt atcgcggaaa agaactgctg ggcttttttg
    68521 aaaaacagat aactgaaagc gcaaaagatc tgactgcaaa agaagattta gcaaccgatt
    68581 tgatttggta tgcgtggagt gggccatttt cgcctttatt cggcaagagt aaaatggcga
    68641 cttttgaacg ttattttctc acagagcctc atacgcacgt cgagaaaagc aatccttatt
    68701 atcacttgcg agatgaagag tggttcagtg aaaaaatttt agcagaattc gacacaaaag
    68761 aagaagggtc agcaatcatt aatgggcaca cgccagtaaa agtaaaaaaa ggggagtcac
    68821 cgatcaaagc aaatggaaaa gtatttgtca ttgacggagg tttatccaaa gcttatcaaa
    68881 aaacgacagg aattgccggt tattcactat tatgcaattc ttatggtttc caaatcgtga
    68941 cacattatcc gtttttgcct attgaacaac aatttgcaaa aaaatccgac caaacaaatg
    69001 tgaaaaaagt aatcgaacag caactaccac gcaaactcaa tcgtgataca acaaaaggta
    69061 tggaactgca gcagcagatt acccagctca aaaggctgtt ggactatcga aaggacgatt
    69121 gagaagaatt ccagtgaaca ttgttccata gactggagac tgagacaaaa gtcgtgtcgc
    69181 agtctctttt tccggataaa cggtgttctt ctgccaactc ctcggtcaca agtcacacta
    69241 ttcaacaaac atgaaaagct gttttttgaa attgctcctt gtgactcaga gttgaacggc
    69301 aggctcacga cctcttggta taaacggtgt tcaaaagcca gtttctcggt attaagccaa
    69361 atttatgcaa aaatgtgaaa agccattttc acaaaatttt tcttaatact cgaaactaaa
    69421 cagctttttc acaaccccct cagaaagtct attaaaattc attcacaaag aaatcgaata
    69481 agcgctgttc agatggatgt acacggtggg taagttccgg atgccattga acaccgagga
    69541 ttttatgcat atcatctttg ctttgaaccg cttcaacgag tccatcgtta gaaaaagcga
    69601 tggcttttaa taaaggtgaa agatctttga ctgcctgatg atggtaagaa ttcacaaaat
    69661 atggatcggg gagaaggttg cttaacaaac tgtctgaaac aatcttgact tcgtgagtcg
    69721 taaattgcgg ggcagtgggc tgttgttcat gttttactga ccactttgga tataatgaaa
    69781 gatcttgata aagtgtacct tccaaaacga cattcaatag ttggagtccc cggcagactg
    69841 caaaaattgg ttttctttgt ttcaatgctt ccaagatcag tgcctgttca aattggtctc
    69901 gttgaatatt tgtttccgtt agctttggat gaggatcttc catgtaaagt tgtggactga
    69961 catcttgacc acctgcaaga ataagtttgt cgatttgtcc aatcagttga ggagctgaat
    70021 ctggagacat gactggcaga ataagaggca atccttgagc atctaaaact gcatccacga
    70081 atccttgcgg tgtatagctc acttgatttc cttggaaagt atctgttgct cgaatcagtt
    70141 gatttccggc aatgccgatg attggtttca tttgtattcc ccctaaagta gacttattgt
    70201 aacaaggatt taaaaaaaat taaactatgt aagacttgcc cctatactta tctgttcttg
    70261 ctgtgttact atctgtaaag agaaatttcg tgtgaaattc atgacgatag ttgaaaatat
    70321 tgcttaattt gttataataa aaagtaagca gaaataaaaa ggttgggcgt aagcttcaag
    70381 gatctagttc ttcaaggggt gagaaggaca ttttattgaa ggggcctgcc tatactaagt
    70441 tatctttaga ggtacatact ttcaatgaaa aaagaaagca tgtacctctt ttttaaatcg
    70501 aaaggaagtg aagcaagtgt tagaagcttt ggaacaaatc cgaaaagcag aagaagaaaa
    70561 tgaaaagcgt gctcaaacac tgcggcagca gctgaagatc tatgcaacag aaaaagagca
    70621 ggaattggaa acgatccaaa caaactatcg acaacaacta aaacagttgg tggaagaaaa
    70681 agaacaattt gaaatacaag aattagaaaa agaaaaaatg aaattgaaag cagaagcaga
    70741 caaggtgcaa gcaactttga aagatcaata tcaaaagcat cagagtcaag cgatcggtgc
    70801 gatcattgaa agggtgaaag aaacttatgg ccgtcactaa gatggaaaaa gtgaccctca
    70861 tttcagataa aagaaatcag gagaggattt tacaagcagt acaaggaatc caaaacgttg
    70921 aaatccggga tctgtatcaa aatagaacaa acaaccagtg ggtggagaag tattttccag
    70981 ataccacgat tattgataaa gaagcaaaat taagcgcttt aggtaatcgg ttgaaagaca
    71041 tccaagaagc tatccaattc atacaacacc acggcgatag ccgtcaaaag aaactccatt
    71101 tgaaacgtca agaactttcc ttacaagatt tggaagccaa ttattccgaa gaaaatttca
    71161 taaataaatt gaaagaagtc cttgatttaa aagaaaagtg ggaacaacta actgaagaac
    71221 gtgatcgttt agaagaagag gaagaatggc ttcttcattg gcaacagctg gatattgctc
    71281 caagtatgta tgcaagtaag acgaccattt ttgagatggg gactgtgagt gcagcaaatt
    71341 ttgaaagttt caaggaagaa ctgagtaagc ttgatgacgt ttatatggaa gaaatagatt
    71401 atacgccaaa acaagtttat ctttcgtatg ttgctttaaa aaaatcagtt gctgttatag
    71461 aagaaattgc tgggaaatac gggttcaata aagaaacgta tccttatgaa caaaatccta
    71521 aagaaaaact gcaagagacc aagcagcaat tatccattgt tcacgagcag caaaagaaga
    71581 taacgacagc gttaggtcag tgcagtggct atatccaaga atttgaatgg gtagaagaag
    71641 tcacacttgc cgttgcagaa cgagaaaaaa taaaagaacg gtttatccat gcgacttatc
    71701 tgatcgtttt gcaaggttgg gtagatgcag aagaaaaaca gaatttgctg catgtgttaa
    71761 acgaaaccgt atcagaagaa gatatatacg tcagtttcga aacaccaaac tcagaagaga
    71821 ttcagcagga agtccctact aaattgaaaa accatccgct tgtcgaacct tttgaattgc
    71881 tgactgaaat gtacagtctg ccaaaatatg aagaagtaga tccaacacct tggatgacac
    71941 cattttatct tgtcttcttc gggatgatgg tcgcagatgt tggttatggt ttgctgatgc
    72001 tcattgggac aatactagct caaaaactac ttgttctacc acgcgggatg aaacgtttcg
    72061 taaaattctt tgagattctc tcgataccat cgatcatctg gggattgatc tacagttcat
    72121 ttttcggaca gtcattacca aaagaactgt ttggcatccg cttgcctttc ccaatcctat
    72181 ctacgacgga tgacgtcaat acgattttga ttttatccgt gattttcggt ctgatacaga
    72241 ttttggttgg attatttgtg gcagcaaagg aaaatatcag gagaaaagat tacttgagtg
    72301 ctgtcagtga tggatttgcc tggcaaggga tcctaattgg gatcgttatt gcgttggttg
    72361 gtgccatgtt gataaaaaat acagcatttg tttaccttgg cgctgggcta tcgatcattg
    72421 ctgccttatg tatcttgatc gttccgatta tccaaacgcc gtctaaagtc aaaggtgctg
    72481 ccaaaggtgc gtataatctt tacggactga ccggctatat cggtgattta gtcagttata
    72541 cacgtttgat ggcattgggt atttcaggcg gaagtattgg agcggcattc aatatgctag
    72601 ttgctttcat gccgccagct gctcgattca gtgtagggat ccttttgatc atcgctttac
    72661 acgcactgaa tatgtttttg acactgctaa gtgcctatgt ccatggcgca cgattgcagt
    72721 atgtcgaatt ttttgggaag ttttatacag gaggcggacg tgcctttgat ccgctgaaaa
    72781 cggcggaaaa atacgtcaac atcaatcaca agaaaaaaaa caataaataa ttggaggatt
    72841 tttatataat gatggattac ttaattagtc aaaatggtgg aatggtattt gcggtattag
    72901 cgatggcaac agcgacgatt ttttcaggga tcggttcagc taaaggtgtt ggaatgacag
    72961 gtgaggcagc agcagcattg acaacaagtc aacccgaaaa attcgggcag gctctgattc
    73021 tgcaattgct tccaggcacg caaggtttgt atggattcgt tattgccttt ttgattttta
    73081 tcaacttagg aaatgacatg tcagttgtac aaggactgaa ctttctaggt gcttctctgc
    73141 cgattgcttt tacaggcttg ttttcaggta ttgcccaagg aaaagtcgca gcggcaggta
    73201 ttcaaatttt agcgaaaaaa cctgaacatg ctacaaaagg aatcattttt gctgcgatgg
    73261 ttgaaacata cgcgatttta ggtttcgtta tttccttcct attagtgttg aatgcctaag
    73321 cataaaggag gaaaagcaga tgaacgccat tgaaaaaatc atctctcaga tgaacgaagc
    73381 agcagagcag gaaagagcgg cgttagaaca agaagagcga atgaagatcg atcaaaactt
    73441 tgaacaaaaa cgtacgcaag ttgaaacaga acatcaaaaa caaaaagaaa aacaaatcga
    73501 attactcgaa aaaaaatacc gtcagctaag gaatcgccag caagtagaag tacgacaaga
    73561 aaatttgaat gcgaagcagg aatttttaag acgattgttt gcagatgcgg taactgaaat
    73621 ggaaaattgg gatgaatctg aacaaatcca gtttatcaaa aatgcattgt attcattacc
    73681 tttgacagga aaagtagcat tcattgctgg agaaaaatca gcagcctatc tttctcagac
    73741 gttgttggat gaatggaaca atgaacttcc ctttatgatg gttttaagtg atgaaactgt
    73801 tgctgatcaa gcgggttttc tgataaacga tcaaggagtt cagtataatt ttcttttcag
    73861 cagccttgta caagatatcc aaggaacgat gagttttgag attgccaatc aattattcga
    73921 ataaggaggg atcagtgtat ggaataccac gaactaaatc ccttgattcg cggaagagag
    73981 cttgaacttt tgtccaatga gacatttgaa cggatgattc aaacaaattc tgtcgaagca
    74041 cttggtgaaa tattgaaatc cacgatttac agtccatata tttatgacgg atatgaatat
    74101 gacttcgaac agaatctttt aaaggagcag agccgtctat ttcgctggtt aaaggaatca
    74161 gcaccggagc ctgaaatcgt ttggatctat acgatgcgtt atactttcca taatctgaag
    74221 gttttgacaa aagctgagat gacaaatcaa aatctggacc atttgtatat cgacgatgga
    74281 ttttattcat tagaaacctt gaaggatgcc attcatacgc aagtttcaaa tgaacttcct
    74341 gaaagcatta tggacagtat acgagaagta cgtgattatt ttgaagagtc taccattctt
    74401 cagggaattg acgtgattta tgatcgacag tttttgacag agcagcgacg gcttgccgat
    74461 aagctaggat atcaagaatt gctgacagaa gtcattgctt ttattgactt gaccaatatc
    74521 acaacgatgg ctcgaggaat attgcagaaa agaacatatg ggttcatgac gacggtactt
    74581 tccagctcgg gaagtatcga aaaagaagta tttttgaact ttgtagataa agatatggaa
    74641 gattacacga attttctatt agagacagat tatgctgatt ttctgcgccc tgtcatccgt
    74701 gagggaaata ttgatctcgt aaagcttgaa cagttgaaag atgattatct aagttcattg
    74761 tatcaagaag cacaaaccca ggctttcgga ccactgccac tactagcctt tttgaacgca
    74821 aaagaggtag aaagcaaaaa ccttcgttta ctggttatcg gcaaaagaag ccatttcgaa
    74881 tcagaaacaa tcagaaagcg ggtgagacaa gtctatgacg cataagatcg gtgtagttgg
    74941 cgacaaagat tctgttttgc cattcaaatt gtttggcttt gatgttcgtt atggtacaac
    75001 caagcaaacc gttcgtcagg caattgaaga aatggcaaag aatgagtatg gcgtgattta
    75061 tgtcacagag caatgtgcag cattgagccc agaaacgatc gaacgttaca aggaacaaat
    75121 gatacctgct atcgtattga ttcccaatca tcaaggatca ttagggatag ggctggaaga
    75181 aattcaaaaa aacgtagaaa aagcagtagg tcaaaatatc ttatagtatg aaaaggagat
    75241 agaaattttg caaattggaa aaatcataaa agtctccgga cctcttgtaa tggctgagaa
    75301 catgtctgat gccagtatcc aagacatgtg tctagtggga gatctaggag ttatcggaga
    75361 aattatcgag atgcgtggcg atgtggcgtc tattcaagta tatgaagaga cttctggaat
    75421 tggtccaggc gaaccggttc gctctactgg tgaagcttta tctgttgaat taggaccagg
    75481 gatcatttca caaatgtttg acggaatcca acggccatta gataccttta tggaaatcac
    75541 acaaagtaat ttcttagggc gcggagttca attgcccgca ttggaccatg agaaaaaatg
    75601 gtggttcgaa ccaacagtag aagccggaga aacggtgagt gctggagatg ttatcgggat
    75661 cgtagaagaa acaaaagtta taaaacataa aatcatggtg ccaaatggga taaaaggaac
    75721 gatcaaaaca atcgaaaaag gttcattcac tattgatgaa acagtatgtg tcattgaaac
    75781 cgaaaatggt gacaaaaatt tgacgatgct tcaaaaatgg ccagtaagac gtagtcggcc
    75841 aatcaaagaa aaattaaacc cggatgcacc catgatcact ggacagcgag tgattgatac
    75901 attcttccca gtgacaaaag gaggagcagc agctgtacct ggtccttttg gtgcagggaa
    75961 aacagtcgtt cagcatcaaa ttgcaaaatg ggccgatgtg gacatggttg tctatgtggg
    76021 atgtggcgag cgtggaaatg agatgacgga tgttttaaat gaattccctg aattgattga
    76081 ccctaataca ggagaatcgt tgatggaacg tacggtattg attgctaata catcaaacat
    76141 gccagtagca gctcgagaag cttctatcta tacgggaatc acaattgcgg aatatttccg
    76201 tgacatgggt tatgacgttg ctatcatggc agactccact tcacgatggg cagaagcact
    76261 tcgtgagatg agcggacgtt tggaagaaat gcctggtgat gaaggttatc cagcttatct
    76321 tggttcacgg cttgcggaat actatgaacg atctggaaga gtcattgcat taggatcaga
    76381 aggtcgcgaa ggaagtataa ccgcaatcag tgctgtatcc ccgtctggag gggatatttc
    76441 ggaaccagtc acacaaaata cattgcgggt cgtaaaagta ttctggggtc ttgattccag
    76501 tttagcccaa aaaagacatt ttccttcgat cgattggata caaagctatt ctctttattc
    76561 aacagaagta gggaagtata tggatcatat ccttcaagct gactgggcat ccatggtcac
    76621 cgagggcatg cgtattttgc aggaagaaca gcagctgaat gaaatcgtgc gtttggtagg
    76681 gattgattcc ctttcagata atgaccgtct gacattagaa gtggcaaaat ccattcgtga
    76741 agattatttg cagcaaaatg cttttgatga tgtcgataca ttcacttcaa gagagaaaca
    76801 attcaaaatg ttgaagatga tcttgacctt tggtgaagaa ggacgccgtg cgttgtcgtt
    76861 aggtgcttat ttaagtgaga tcatggacgg taccgtatcg attcgtgaaa ggatcggacg
    76921 aagcaaatat atcccggaaa cagaattaga aaaactagat caaattattg gtgaaatcaa
    76981 gcaaacgatg caaacgatcg tttcagaagg aggcatgagc aatgattaaa gaatatcgta
    77041 cgatcggtga agtcgtcggt ccattgatgg cggttgaaaa agttgctggt gtcaaatatg
    77101 aagaattaat cgaagtccgt atgcaaaatg gcgagattcg tcgtggacaa gtattggaag
    77161 tccaagaaga taaagcaatg gtccaaatat tcgaaggaac aagtggtatc aatttacgtg
    77221 actcttctgt acgattttta ggccatcctt tagaactagg tgtatcggaa gacatgatcg
    77281 gtcgagtatt cgatggtttg ggacgaccga aagataacgg accagagatc ctgcctgaaa
    77341 agcatttgga tattaacgga gaagtaatca atccagtagc tcgggattat ccagatgagt
    77401 ttatccaaac aggtatctct gcaatcgatc atttgaatac attagttcgt gggcaaaaat
    77461 taccagtatt ttctggctct ggtcttcctc ataaagaatt agctgctcag atcgcgcgac
    77521 aagcaacggt actcgattca tcagatgatt tcgccgtagt ctttgctgct attggaatca
    77581 cttttgaaga agctgaattt ttcatggaag attttcgtca aacgggcgca atcgaccgtt
    77641 ctgtcatgtt tatgaattta gcaaacgacc ctgcaatcga acgtatcgct actcctcgta
    77701 tggctttgac tgctgcagaa tatcttgctt atgaaaaggg aatgcatgtt ttggttatca
    77761 tgacagacat gaccaactat gcagaggcat tgcgagagat ttctgcagca cggcgagaag
    77821 ttccaggaag aagaggatat cctgggtatc tttacacgaa cttagctact ttattcgaac
    77881 gagcaggaag aatccgcgga ctaaaaggat cggttactca aattccgatt ctaacaatgc
    77941 cagaagacga taaaactcat ccaatccctg acttgactgg ttatattaca gaaggacaga
    78001 tcatcttgac tagagagctt tacaagagtg ggattcagcc accaattgat gtactcccgt
    78061 ctctttcgcg gttaaaagat aagggaactg gacccggaaa gacaagagaa gaccatgcag
    78121 ctacgatgaa tcagcttttt gcagcctatg cacaaggaaa acaggcaaaa gaattagccg
    78181 tagttttagg tgaatctgct ttatctgatg tagacaagat ctatgcgaag tttgctgagc
    78241 ggtttgaaaa agaatatgtc aatcaaggat tctacacgaa ccgttcgatt actgaaacac
    78301 ttgatctagg gtgggaactg ctatcgatgt tgccaagaac cgaattaaaa cgaatcaaag
    78361 acgacatgct ggacaaatat ctgccagaag ggaagtgagt tctgatggca cgattgaatg
    78421 tgaatccgac ccgcatggaa ttgactcgat tgaaaaaaca gttgacaact gccacaagag
    78481 ggcataaatt attaaaagat aagcaagatg aactgatgcg tcaattcatc ctgttgattc
    78541 gaaagaacaa ccagctgcgt caagaaatgg aagagtctat gacagaagca atgtctgatt
    78601 ttgtattggc aaatgcttcg atcacggaac ctttcatcga agaattattt gttttgccgg
    78661 cggaaaacgt tgatttgtct atcgtagaca aaaatatcat gagtgtaaaa gtacctgtga
    78721 tgaattttga atatgatgaa cagctgacag acacaccttt ggaatacggg tatttgaatt
    78781 ccagtgccga actggatcgg tcaatcgatc ggtttactac cctgcttcca aaacttttgg
    78841 aactgacaga aatcgaaaaa acttgtcaac tgatggcgag tgaaatcgaa aagacacgtc
    78901 ggcgagtcaa tgcattagaa tatatgacga tccctcaatt agaagaaacg atctattata
    78961 ttcgtatgaa gctggaagaa aacgagcgcg cagaagtaac aaggttgatc aaagtaaaaa
    79021 atatgggtac cactgactaa gtggtatctt tttttaaaag aggaaaacga tgattaaaca
    79081 aagcaaacaa ataaagaaac gtttgccggc tgttcagctt ctcgcattag gattttttct
    79141 cctgatttta gttggcggcg gcctacttac cctaccattt tttagtaata gtgggcatgg
    79201 gacaaatttt atcgatgcgc ttttcactgc aacttctgct gtctgtgtaa cgggattgac
    79261 aacattgaat acagcagagc attggaacag tgctggtcaa tttttgatca tgcttttgat
    79321 tgagatcggc ggtcttggtt ttatgatggt tccgattcta tgctttgcat tgataaagaa
    79381 gaaagtcagt ctcagcacac gtattatatt aaaagaagcg ctgaatctag aagaagtatc
    79441 aggtgtgatg aagctcatgc tttacatcct aaaattggcc tttgtgatcc aattgattgg
    79501 tacggttgct ttatcttttg tatttgtgcc tgaatttggc tggttgaaag gtatctggta
    79561 cagtttgttc catgcaatct ctagtttttg taatgcaggg tttgatttgt taggagacag
    79621 tttagccaat caccagcaaa atgtttattt gatcatggtg gtatctgctt tgatcatttc
    79681 tggtgggtta ggcttcatcg tatggcgtga tttactggaa tatcatcaga aaaagaagat
    79741 gacgattcat tcaaaaatcg cattaaccgt tacaggtacc cttttattag gcggattcgt
    79801 tgtgttcttt ttcacagagc gcaatggggc aacgctagtg gaagggaata tgtttcaacg
    79861 attggcaaac acctttttta tgagtgtgac acctagaacg gctggatatt attcagttga
    79921 ttacctccat atgagccatg caggtctgat tttaacgatg tttttgatgt acatcggcgg
    79981 aacatctggg tcgacggccg gcggattgaa aacaactacg ttaggtgtgt tgctgatcca
    80041 gatgcgttcg atgttgaaag gacgtacgag agcggaagca tttggccgta ccatccgaca
    80101 aggcgcagtg ttgcgggcac ttactttatt ttttgttacg ttgacgttgt gtgtagtcgc
    80161 catcatgatt ttatcggtga ctgaaacgat acctaaaact tctggcatcg aatatattgc
    80221 ttttgaggtc ttctctgcgt ttggtacagt aggtcttacg atgggattga cgccagactt
    80281 gactgcaata gggaaattga tcattatagc gcttatgtac attggacgag ttggtgtatt
    80341 gactgtggtc ttctctctga tgacaaaagc acaaagaaca caatcaaaat acaaatatcc
    80401 agaagaaagt gtcatgattg gataatttca tcttctgaca gaaacctagt gatttagtaa
    80461 aataagaaaa taaaataatt tttcttgctt cttggatact ttaagtgcaa aatgttcgtt
    80521 tattttttga ggcactaccc gttgttgaac ctcgggtagt gcctcttttt tattgaaagg
    80581 aatcaataag atttgattcc tttatttatc aataggttat aattcatatt tttttatttt
    80641 ttcttttacg atacttagtc cagaagctaa aatttcaagg ttattagagg atagggaaat
    80701 tcgaacgaat gcatctggtt gcgcacgtgt agtaaagcgt ctggaatgat aaagtcggat
    80761 accgttagca agaaagtcat tctcaattat cttagggtca atctcaggat caactggaag
    80821 aattcgatag taagggacac gattcgttgg tttttctact ttgaaaagag aatcaaaaag
    80881 ctgattggct ctttttgtaa gacttaattt agtttctaag atcctatttg cttcatttga
    80941 atggaggatt tgagtgatga cttccacatc taatccagaa gttttcacat ttatattaaa
    81001 aattgcttgt tctatcttgc tttggacatg tactggaact acaagataag caactcgcaa
    81061 tccagaacaa ataaattttg tcattccagc taagtagatt gtttggttag gtaataaatt
    81121 ttggaaagga ggaatgatcg tatgttgata gtaagttgtt aaaaatgaat ggatatcatc
    81181 ttcaataacc catagacctt ctttttcaat cacttcggct aattgctctc gtcttttgtg
    81241 agagatttga aaccctatag gattattaca agaaggcata agaaagacac catgaatctt
    81301 cttttttcta cattcctgaa tcaatatatc aggtcgcatt ccttgctcgt caaaatcaat
    81361 tggaacgatc tctaaatgat ataactgagc caattcaatg aaattagcgt atgtatatcg
    81421 gtctgttgct attctatcac ctggagagaa aagtgcagaa agtgtgatag ccaatccatt
    81481 ttgaacaccg gaaacaatag atagagattc taaggacgga atgactcctt gagtttttaa
    81541 ccaatttgct gcagtagtca attgaaaatt tgtgcctttg ggatcttggt aattaagcaa
    81601 accaacaaga tttgggttat tactagttga ttgaataaac gggacaacga atttattaca
    81661 ttcttcaaaa gaactaacca gaccaaaatc aatgatatgt tcggttactt cattggtaga
    81721 gatggtgatg gactcaaaga cattgggaga aataaatgtg ccacttccaa tattcgtata
    81781 cagcaaacct ttctcaattc catatttata agccttgcca acggtagtaa aatttaaatc
    81841 taaataatca gctagctctc tttgggaggg taactgagtg tttttctgta atttattctt
    81901 taaaatatct tgttctaatt gttgaatcaa cgatcgataa taaggacgct tcaatttttg
    81961 tttgtctggt cgccaagtta acgggtaatt gtcataagaa ttaataggca ttttctccac
    82021 tccttaaaat tgtataccat acaattttac cattgattgt attgatttta gaagttaatc
    82081 ttttgtgagg agggaattgt ctatgaaaat tattaatttt gaggaagtag tagcttcttt
    82141 tagttttgaa gaagcgattc aagtaatgaa aaaatgtttt tttgattatg aaagtggtaa
    82201 aatcagccaa aatcctcgat tggtggaaat tttacctgac ggaaaaggca aaaatatgtt
    82261 tgcgatgatg cctgcttatt tgggggagaa cagatatttt ggcacaaaag tcattaccgc
    82321 ttttccggat aatcatcagt atcatttacc ttctcattta ggagagattt tcttgtttga
    82381 tgctcaaaac ggactaccca aagcattgat aaacgcaaat gcagtcacct ggattcgaac
    82441 agctgctgta tctgcgctgg caacagatta cttagcaaat ccgcatgcag aaacacttgc
    82501 tttaattgga gctggtcaac aagcattgtc gcatttgact gcgatttcta ccattcgtcc
    82561 catcactaca gtgtttgttt acgatatttt agaagaaaga cgaaatactt ttatcgctaa
    82621 agctaaaaaa cagtatccta aaataacgtt tataaatgca atatcagtag aagatgcagt
    82681 acgtccagcg gaaatcattt gtacgttgac ctctagtaaa gaggcatttc ttcaaaaaga
    82741 gtgggtaagg gaagacgcac atatcaatgc tgtgggcaca tttactcctg atacaagaga
    82801 gataagtaca tcgttgatgg aaacttctca aatctatgta gatgattact cagctgcctt
    82861 aacagaaagc ggggacctgc ttatagccat ggcagaagga gatttagagg aatcttgtat
    82921 tgttggtaca ttaggcgaat tagtttctga gaaaaaaaca ttttataaag gagaatcgag
    82981 gccgacgatt ttcgacgcag ttggtctagc tattgaagat ctttgttgtg cagaatatat
    83041 ttataacaaa ttagagaaag gggctttaaa atgaacttag attattacgt agttgatgca
    83101 tttgcagatg aggtgttcaa aggaaatcca gcagcggttt atgtgctaga agaatggctg
    83161 ccagaaggta cgatgcaaaa aattgccatt gaaaacaatt tatctgaaac agcatttaca
    83221 gtgaagaaaa accaagaatt tgagcttaga tggtttacgc cagatcgaga aatcgatctt
    83281 tgtggtcacg cgacattagc aacagcattt gttttattta actattacaa aatacctgat
    83341 gaaacgatta aattttctac tcaaagcggt aatctatttg tgacaagaat taaagattat
    83401 tattacatgg attttcctag tattatgccg aaaaaagtgc cgatattgtc tgaatatgaa
    83461 gaagcgattg gggctaagat taaagaagcc tacttagcac gtgatttatt tttcgtatta
    83521 gaagatgaaa aaacggtggc taaattgcag ccagatttta cagctatcaa aaattttgaa
    83581 ttaggaatag gagtcatcgt gacagcagaa agtgtgcaaa aagactttgt ttctagaacc
    83641 ttctttccaa aattaacaat caatgaagat ccagtatgtg gatcagcaca ttctaatctt
    83701 attccttatt gggcagaaag aatgaataaa acaaaattgt cagcttatca aatgtcatca
    83761 aggggcgggg atctagaatg tgaattaaaa gaagatcgtg tgataatagg aggaaaagca
    83821 gttttgtttt ctaaaggtac ctcgtttatt tgatttcttt caaatgaatt aaaaataaat
    83881 tggtggagag tggcaacctt atagctacaa tggttgttaa aaaaagacgc aaactttttc
    83941 aatttaatga aaagtttgcg tctcatttta attattggtg tttgtttttt attcaatcaa
    84001 gagtaaaatg ccccatacta actatcctcg gatgacttcg attttataac catcagggtc
    84061 aatgacaaag tagtaagaag gcgcagtgcc aggtaatcct tttaagtcag taatctcaag
    84121 accagcagct ttatgttgct cgtgtaattt ttctagatca tcagctgcta tagcgatatg
    84181 accatagccg tctcctagat catacgcacc atgatcatag ttgtaagtca actctaattc
    84241 atagtcatcc ccaggaagtg tcaaataaac aagtgtaaat ttgttttctg gaaaatcacg
    84301 gcggcggctt tcttcaaaac caaatgcctt ttgataaaat tcgattgatg cttcaagatc
    84361 tttcactcga acacatgtgt gagccatttt catagattca tctctccttt tccttttact
    84421 caatcataac aaaaagaagt aaggaaatag ttgatttgtg cttgttcatg agaaatttct
    84481 catgtaaaat ggatagaaaa taaaactgtt aaggtggaaa ctattcatgg ataaaccaat
    84541 ttttggtgaa aaaaagcaat ttgctgatta tcaaacccgt tatgcagcat atattgtcat
    84601 agaaagagat cagcagattg cagtaatcga ggctccaaat ggtgcttttt ttcttcctgg
    84661 cggggaaatt gaaggagaag agacgaaaga agaagcgatt gtccgtgagc tgatagaaga
    84721 aatgggaatt gtggcagaaa tttcttctta tctgggagaa gctgatgagt atttttactc
    84781 gaattatcgt gcaacttatt attataatcc aggatatttc tttgttgctg attcttggaa
    84841 gcgtatagga gaaccaactg aaaagaccaa taaaatttgg tgggtgagcc ctcaagaagc
    84901 gcttgtgaaa ttgaaacgtg gtagccaccg atgggcagtc gagagatgga tcgaaaaaaa
    84961 taaaaactga aagaaagcga gtagatcagc tttctctcag tttttttatt tattacagta
    85021 catatttttt gattgcttca gctacaccgt gctcgtcgtt tgatgaagtg atgacatttg
    85081 ctgcttcttt gacaagagaa acggcatttc ccattgctac tcctagacca gcatattcaa
    85141 tcatggataa gtcgttctcg ttatctccga tagccatgat ttcatcttga tcaataccaa
    85201 ggtgctgtgc gagattcgca actgccgcac ctttacttgc ttctttattt agtacttcga
    85261 agtaataggg cgcacttttg accgttgtgt atttatcacg gaaagtttgc ggtaaacgcg
    85321 caattgctgc atctaaaatt tctggttcgt cgatcatcat cattttgacg atgttcatgt
    85381 ctggtgtcat ttcttcaggt gttcgatatt ttagcggcat cttgactaaa cttgcttcgt
    85441 ggatcgtata agcgctgata tctcgattag gcgtgtagat cgctttgtca tcgatcgaat
    85501 ggagatgact gccgacacgg cgtgccatta cttcgatctc taggaaatct tcatgtgtca
    85561 gtccgtgacg ggcgatgatt ttacctgatt tcgtttgttg tacgagtgcc ccgttataag
    85621 taataacgta atcattatct tgaaaaaggt tcaactcgtt taactgttcc tggacacctg
    85681 gtaagggacg gccagtgcat aaaacgatcg aaacaccttt tgcaacagca gcggcaatcg
    85741 cttcttttac ttcttttgtt acttctcttt tctcatttaa taaagtacca tcgatgtcga
    85801 tagcgactag tttgatagac ataatttcct ccaatatatt ttaatttatt tcaatcaatg
    85861 cgccattacg gatatggctg gcaaattctt gataagtttc gtcaaataaa tcataatgat
    85921 cacgcaagga cggatcaacc atttcttttg gaaagaaaaa tcgctcatct ccttgttctt
    85981 gtcccgctaa tgcagcaaca agctgactaa cctgtgataa ttctatcaaa gttccgtcat
    86041 ttcgcaaaat ctcgatttgt gtgcgatgtc tgccttgttc tgggcgataa aaatcataag
    86101 gtagatcgta gctagaattc acagcagtgt aataaacagg attatatccg accttttcta
    86161 ctaacaaagt gagttcttgg actaattttg aatcccggtt gccagaaaaa gtagcagatt
    86221 tcagtggttt acgattcaaa aaacgtttag ctaaatcatt taaaatagga tcagcagatt
    86281 cagaccattg agtaaagtaa gtgcccaaga ccccatcatc aagtttgagg tattcttcta
    86341 aagtaaagct tccgtcaaga aatggcagaa gtaattgaga atggagttca aaactgctgg
    86401 catcttcttg atacaactcg tgtgcgcgat gaagcagatg ctccaaaatt acttccatac
    86461 cacgggaaac aggatggaag tacacttgca catacatttg atatcggcta acgatataat
    86521 cttccactgc atgcatgcca ctcatggaaa aagcgatccc gccttcataa ggacgaatca
    86581 cacgtaatat tcgggtaaga tcaaatgttc catattctgt tccagtgaaa taagcatccc
    86641 gaagcaaata gtccattcga tctgcatcga tctgactaga aatcatttgg acaacttgtg
    86701 ggttggggta agttttttgg atcacactag ctaccttttc aggaaaacct tcttctactc
    86761 ggttaagaat ctgatacact tctgtttctg gagaagtgat gatctctaca gtgatggctt
    86821 catgattcgt atggaagata tgttcaaatg tatgagagta aggaccatga ccgacatcat
    86881 gcaacagtgc cgcacataaa gtcaccagcc gttcctgatc atcccagcct ccgttgccta
    86941 ttttttcttt tgaaaaattt cgagaaaaga tatcgcagat tcgtcttgaa atctcataaa
    87001 cgcctaatga atgagtaaaa cggctatgtt ctgctccgtg aaaagtgaaa gaagaagtgc
    87061 ctaattgttt aatgcgtcgt agacgttgga cttctttcga attgatcaaa tctaagatta
    87121 cttgatgctg gacatggata tagttatgga ctggatcacg aaaaactttt tccataggca
    87181 gcatttgata tttataaggg gttgtcataa tgtagctcct ttatgttatt acgttgttcc
    87241 attctctgga tttggttgtt ccattgctcc agctggattt ctttttttag ccagtcggta
    87301 gaatcctgta aatcacttgc agaatacagc ttttcaaaag cttttgcaaa tgcttctttg
    87361 acttctttta caaaaagggg ttggtcaagc agttcttcaa gtgttgccat tgtagcagga
    87421 tccactgcag gatagccatt tgtaccaaag tcttctttta aagcagattg ataaaatcgc
    87481 ttcatgactt gcccacgttt ttcctgatta ccgttgacac ttaagtacat cataacagaa
    87541 atcccatttt tgatccgacg ctgagcgata ccggcaaatt ttttgccatg gatgcttaaa
    87601 tcataagttc caggacaata agaatgggtg atttcgaatg cttgtatcgg tgcctttgga
    87661 aaagcgtatt tagtcaatag gagcatttgc tcgtaagcgt catctatggt tatttttttt
    87721 gtgtcacttt gggggaaaat caaagaaata ttcaaaattc cttcatctga tacgatccct
    87781 aatccacctg aatttctgat cactggagcg taacctgcat cttccagtgt ttggatcccg
    87841 tcacttagaa aaggagttcg aacatccttc atacctaaga tgactgtgca gtcatattgc
    87901 cagaaatgta aaaagatttg ctggtgttct ttagaatatt ccgttaatat atctgttaat
    87961 gcaaaagggg aaaagtcgtt tttttgataa atgtgctgat ctagtcgaat cgcagggttc
    88021 attcaaccgg ctcctttgta taaattcata ttttctacaa tattatactc taaaatcaaa
    88081 gagttgtttt ggaaaaaagg ttttctgtaa aaatggacaa gttggttgac aaaacaaaag
    88141 aaactaggaa caatgtagct atgagaagta aaggagaatg aataatggaa ttatcacaag
    88201 gaacaccgat ttcaatcaaa ttgcgtacaa aagtaaaaca gcaaggggaa gtgcaagatt
    88261 tttattttga tttgaaaggg caaatggtga caattggcga tacgctgtac atacgttata
    88321 aagagattca ggaggagacg ggcgatgaga ttcctgtaac gatcaaatta gttccagatg
    88381 gtcatgtaca gctgatccgt gcaggagaaa tgcggatgcg tttgaaattt ggctataaag
    88441 aacggttaga aacatcctat cgaacaccat acggtatgat ccagattgct acttttacga
    88501 aagaactgca tgttagtttg aaagaccgtc ccacagcagg gaaggttcga atcgattatg
    88561 atctgttcat gggaccggaa aaaatcggcg aatattattt gaccttggat tttacagcgt
    88621 aaatataaaa gaaaagtttg attttttgtg tctgattttg tatgataagg agtagactgt
    88681 gaaaggacgt gtcctgattg gaaattagtg tatttgaagg tgcaaacaaa agcgaattat
    88741 caatgatcga agtagcacac gcgatcttag aacaacgtgg cgatgtcatg gatttttctg
    88801 atttagctaa ccagatccaa aactatcttg ggaaatcaga cagtgaaatt cgtgattcac
    88861 tcgcacaatt ttatactgac ttgaatattg acggcagctt tatctcatta ggggataatc
    88921 gttggggctt gcgttcatgg tatgctatcg attcgatcga tgaagaagta aaccatggaa
    88981 tggacgaaga ggatgaagat actccacgtc gccgtaaacg caagaaagtc aacgccttta
    89041 tcaatgatga tgaagacgcg atcgattaca atgacgatga cccagaagat acagatctga
    89101 cagaagaaga tgacgatgat ctgtttgatg acgatgatga tgaagatgaa gaaatcgctg
    89161 cttataattc tgacttgcaa gaaatcggag cagatgaccc gacagatgat gaagaagact
    89221 tgccaggaaa tatcgaagaa gatttaagta tcatagacga agacgatgat gatgaagatt
    89281 atgaagagga agaattttct gactctgaaa aagaataaac attattttct tacgagaggt
    89341 tgtgacagag acgatcagcc tatcagaata aggaggtgtg acacgatttt tgtcacacct
    89401 tcttttttgt atataaaaat cccaagtaaa tacataaaaa ataaaaaaat tgaaactttt
    89461 agcgcacctt gtacgtcata ttaagtgagg cagaaaggag tggagaatga tttttactgg
    89521 atataagaaa aaacggcaga tcgaaaaata ccgtcacctg ctgaatggac tcatcgaaac
    89581 tgattatcaa aaaatgttcc gtatcacgat gaattacgta catgataaag aagaggcctt
    89641 agatgtgatg caagatagtt ttcataaagc cctgaactat tttgaaaaag aaaaagagat
    89701 cgagcatttt agtgcatggt tttatcggat attgatccgc acggcattag atagttggcg
    89761 cagacaaaaa aggacgcctg tggacttatt tgaaattcaa gatatccccc aacagttacc
    89821 tttagaaact tccgttgcag agctccatat gattttagat aaaatagaca gtccggaaaa
    89881 agagattctg atcctctact tttttgaagg atttcgtttg aaagaaattg cgactatctt
    89941 ggatatgaat gaaaacacag ttaaaacgaa aatgtaccgc tcattgaatg ctttacgtca
    90001 aatcctagaa tgaaggagag aagagtgcca tgtattccca aaaagaaaat ttaaaaaaac
    90061 taaagaaaac ttataaagaa caagcaattc ccaaagggct taaagaggag atccgccagc
    90121 gttttattat cgaagagcaa tcatttataa aagcaaagcg gagaaagaga cgtctccaag
    90181 ggttcggagt atctttggct atcgtaatga ttacaacaag tagccttctt tttaatagtc
    90241 aagttcgaag ctttgcagaa gatttaccca tcttaggatc agtcattgaa ttgatcttag
    90301 gcgaacgctt tactgatcga tctgaaaaaa tcgatattca ggtaccgaag atcaatacac
    90361 aaaatgagaa agaaaataaa acaattcatg gattaaatca aaaatacttt cgcgaaggac
    90421 aagcagattt tgaaaaagct gaaaaagaat atggaaattt tgaaacggat cattaccaag
    90481 ttttgggaga ttatcagaaa atcgtagacg ataaccggtt tctcgtgatt gaaaggcaga
    90541 tcacccaaac agcagcagat agccatgttg aaaaaagata cgacacaatc gacaagaaaa
    90601 acagtgtcca gctctcactt ccacttttat tcaaagatga cacctatcta tctgttttga
    90661 caaaagaagt caaacggcag atggccgaac aagtcaagga agatccatct aagtactatt
    90721 ggactgagca agatattcaa gaagggacga tagaaaaacc aacacttgtt acgcctacac
    90781 gaagttttta tttgaacaaa gagcatcaat tagttttgac tttttctcaa tacgaaattg
    90841 ctcctggtta tatgggtaca ccagaatttg tcattcctaa gtccgtgaca aaaacgattt
    90901 tggcatcgga agattatttg gatcattaga cgagaagtag ttgcgactag cagtttcaat
    90961 tttcaagtaa gtacaagtaa ataataagaa gagaaaaaga gaaagaagag atatagtgaa
    91021 aagaaattat ttagttatca gtattattgc tttatccgtg cttttgtttt tcgaaattac
    91081 ttcggtggcc tccttgatca ccgaagtaaa aggacaaaca cactcttcgg agcattcgtc
    91141 acaatctttc aaacaatcag aacaatcaac cattaaaagc caatcaactg aaacagctaa
    91201 cgtcaaagaa gccattcaag aatcagcgga aaaggtacaa agtcagtcat cacaatcaac
    91261 acaagacagt acaaaaacga gccgagatcc ggctgtcaaa agcgtcgcaa tcagctttga
    91321 tgatggacca ggagcgacca caacaccgca gcttctgcga atcttaaaag aaaaaaacgt
    91381 gcatgccact tttttcgtct taggagaaaa tacggcgcag catcctgaaa tcgtgaagca
    91441 gacagctgaa gcagggcacg agattggcaa tcacacatac gatcatcaga atctggccac
    91501 tctttctgcc cagtcgatga cggaggaagt gacgaaagca gacactgaga tcaaaaaagt
    91561 cactggcaaa acccccactt ttgttcgtcc gccctatggt tctgtcacga atgtaggagc
    91621 aacgatcatc cagcgaccga tcatcgaatg gtctgttgat tcagaagatt ggaagacaag
    91681 aaatcctgat ttgattttgc agaagatcca agcaacagta tacgatgggg caattatttt
    91741 attccatgat atctatcctg aaacgattcg tgccgttcct caagtgatcg attatctgaa
    91801 agagcaaggg tatcgcatca cgacagttgg ggatttatta gggcatccaa cggctgtcga
    91861 gaactattat ggcagaaacg atcatcggcc agttcagtaa gtgagacaag tatctttaat
    91921 aaataacaca ttttaataag tagttgaaca cataactacc ttacttgcaa aggcttaaca
    91981 atatctatac aggtacaaca aaaaactgtc caccaatttt
//
LOCUS       DANJMI010000005        78080 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000005 DANJMI010000000
VERSION     DANJMI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.005"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(407..1576)
                     /locus_tag="PWU81_000426"
     CDS             complement(407..1576)
                     /locus_tag="PWU81_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HDL1109161.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            complement(1647..2197)
                     /locus_tag="PWU81_000427"
                     /pseudo
     CDS             complement(1647..2197)
                     /locus_tag="PWU81_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
     gene            complement(2400..2879)
                     /gene="rlmH"
                     /locus_tag="PWU81_000428"
     CDS             complement(2400..2879)
                     /gene="rlmH"
                     /locus_tag="PWU81_000428"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL1109162.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            3307..4596
                     /locus_tag="PWU81_000429"
     CDS             3307..4596
                     /locus_tag="PWU81_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDL1109163.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4635..5240)
                     /locus_tag="PWU81_000430"
     CDS             complement(4635..5240)
                     /locus_tag="PWU81_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDL1109164.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5445..5909)
                     /locus_tag="PWU81_000431"
     CDS             complement(5445..5909)
                     /locus_tag="PWU81_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL1109165.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5931..6251)
                     /locus_tag="PWU81_000432"
     CDS             complement(5931..6251)
                     /locus_tag="PWU81_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL1109166.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(6395..7474)
                     /gene="pepA"
                     /locus_tag="PWU81_000433"
     CDS             complement(6395..7474)
                     /gene="pepA"
                     /locus_tag="PWU81_000433"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HDL1109167.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7674..8024
                     /locus_tag="PWU81_000434"
     CDS             7674..8024
                     /locus_tag="PWU81_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL1109168.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            8247..9194
                     /locus_tag="PWU81_000435"
     CDS             8247..9194
                     /locus_tag="PWU81_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL1109169.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9251..10669)
                     /gene="pepV"
                     /locus_tag="PWU81_000436"
     CDS             complement(9251..10669)
                     /gene="pepV"
                     /locus_tag="PWU81_000436"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDL1109170.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10818..11663
                     /locus_tag="PWU81_000437"
     CDS             10818..11663
                     /locus_tag="PWU81_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDL1109171.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11667..12107
                     /locus_tag="PWU81_000438"
     CDS             11667..12107
                     /locus_tag="PWU81_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDL1109172.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            12157..12351
                     /locus_tag="PWU81_000439"
     CDS             12157..12351
                     /locus_tag="PWU81_000439"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109173.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12487..13164
                     /locus_tag="PWU81_000440"
     CDS             12487..13164
                     /locus_tag="PWU81_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1109174.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(13572..14261)
                     /locus_tag="PWU81_000441"
     CDS             complement(13572..14261)
                     /locus_tag="PWU81_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109175.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(14236..16365)
                     /locus_tag="PWU81_000442"
     CDS             complement(14236..16365)
                     /locus_tag="PWU81_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDL1109176.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(16413..17750)
                     /locus_tag="PWU81_000443"
     CDS             complement(16413..17750)
                     /locus_tag="PWU81_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDL1109177.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(18002..18361)
                     /gene="rplT"
                     /locus_tag="PWU81_000444"
     CDS             complement(18002..18361)
                     /gene="rplT"
                     /locus_tag="PWU81_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL1109178.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(18414..18614)
                     /gene="rpmI"
                     /locus_tag="PWU81_000445"
     CDS             complement(18414..18614)
                     /gene="rpmI"
                     /locus_tag="PWU81_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL1109179.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18686..19210)
                     /gene="infC"
                     /locus_tag="PWU81_000446"
     CDS             complement(18686..19210)
                     /gene="infC"
                     /locus_tag="PWU81_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL1109180.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(19254..19379)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19530..20162
                     /locus_tag="PWU81_000447"
     CDS             19530..20162
                     /locus_tag="PWU81_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL1109181.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(20164..21069)
                     /locus_tag="PWU81_000448"
     CDS             complement(20164..21069)
                     /locus_tag="PWU81_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL1109182.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(21154..21813)
                     /locus_tag="PWU81_000449"
     CDS             complement(21154..21813)
                     /locus_tag="PWU81_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL1109183.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            22129..23073
                     /gene="mvk"
                     /locus_tag="PWU81_000450"
     CDS             22129..23073
                     /gene="mvk"
                     /locus_tag="PWU81_000450"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDL1109184.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23066..24043
                     /gene="mvaD"
                     /locus_tag="PWU81_000451"
     CDS             23066..24043
                     /gene="mvaD"
                     /locus_tag="PWU81_000451"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDL1109185.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            24056..25141
                     /locus_tag="PWU81_000452"
     CDS             24056..25141
                     /locus_tag="PWU81_000452"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDL1109186.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            25138..26202
                     /locus_tag="PWU81_000453"
     CDS             25138..26202
                     /locus_tag="PWU81_000453"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDL1109187.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26451..27254)
                     /locus_tag="PWU81_000454"
     CDS             complement(26451..27254)
                     /locus_tag="PWU81_000454"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDL1109188.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(27274..28116)
                     /gene="kduI"
                     /locus_tag="PWU81_000455"
     CDS             complement(27274..28116)
                     /gene="kduI"
                     /locus_tag="PWU81_000455"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDL1109189.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(28132..29139)
                     /locus_tag="PWU81_000456"
     CDS             complement(28132..29139)
                     /locus_tag="PWU81_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL1109190.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(29146..29790)
                     /locus_tag="PWU81_000457"
     CDS             complement(29146..29790)
                     /locus_tag="PWU81_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL1109191.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29890..30648)
                     /locus_tag="PWU81_000458"
     CDS             complement(29890..30648)
                     /locus_tag="PWU81_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL1109192.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30775..31926)
                     /gene="fucO"
                     /locus_tag="PWU81_000459"
     CDS             complement(30775..31926)
                     /gene="fucO"
                     /locus_tag="PWU81_000459"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL1109193.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(32008..32319)
                     /gene="rhaM"
                     /locus_tag="PWU81_000460"
     CDS             complement(32008..32319)
                     /gene="rhaM"
                     /locus_tag="PWU81_000460"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDL1109194.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(32321..33166)
                     /gene="rhaD"
                     /locus_tag="PWU81_000461"
     CDS             complement(32321..33166)
                     /gene="rhaD"
                     /locus_tag="PWU81_000461"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDL1109195.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(33180..34436)
                     /gene="rhaA"
                     /locus_tag="PWU81_000462"
     CDS             complement(33180..34436)
                     /gene="rhaA"
                     /locus_tag="PWU81_000462"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDL1109196.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(34433..35893)
                     /gene="rhaB"
                     /locus_tag="PWU81_000463"
     CDS             complement(34433..35893)
                     /gene="rhaB"
                     /locus_tag="PWU81_000463"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDL1109197.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            36046..36915
                     /locus_tag="PWU81_000464"
     CDS             36046..36915
                     /locus_tag="PWU81_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HDL1109198.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(37041..37940)
                     /locus_tag="PWU81_000465"
     CDS             complement(37041..37940)
                     /locus_tag="PWU81_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1109199.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(38105..38929)
                     /locus_tag="PWU81_000466"
     CDS             complement(38105..38929)
                     /locus_tag="PWU81_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL1109200.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(38940..40235)
                     /locus_tag="PWU81_000467"
     CDS             complement(38940..40235)
                     /locus_tag="PWU81_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDL1109201.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(40251..40730)
                     /locus_tag="PWU81_000468"
     CDS             complement(40251..40730)
                     /locus_tag="PWU81_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL1109202.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(40736..41764)
                     /locus_tag="PWU81_000469"
     CDS             complement(40736..41764)
                     /locus_tag="PWU81_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL1109203.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41781..42887)
                     /locus_tag="PWU81_000470"
     CDS             complement(41781..42887)
                     /locus_tag="PWU81_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL1109204.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(43116..45713)
                     /gene="adhE"
                     /locus_tag="PWU81_000471"
                     /gene_synonym="adhC"
     CDS             complement(43116..45713)
                     /gene="adhE"
                     /locus_tag="PWU81_000471"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL1109205.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(46018..46398)
                     /gene="yajC"
                     /locus_tag="PWU81_000472"
     CDS             complement(46018..46398)
                     /gene="yajC"
                     /locus_tag="PWU81_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL1109206.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(46495..47640)
                     /gene="tgt"
                     /locus_tag="PWU81_000473"
     CDS             complement(46495..47640)
                     /gene="tgt"
                     /locus_tag="PWU81_000473"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL1109207.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            47858..48556
                     /locus_tag="PWU81_000474"
     CDS             47858..48556
                     /locus_tag="PWU81_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL1109208.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(48724..51363)
                     /locus_tag="PWU81_000475"
     CDS             complement(48724..51363)
                     /locus_tag="PWU81_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL1109209.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(51982..53013)
                     /gene="queA"
                     /locus_tag="PWU81_000476"
     CDS             complement(51982..53013)
                     /gene="queA"
                     /locus_tag="PWU81_000476"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL1109210.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            53206..54381
                     /locus_tag="PWU81_000477"
     CDS             53206..54381
                     /locus_tag="PWU81_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL1109211.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            54385..55026
                     /locus_tag="PWU81_000478"
     CDS             54385..55026
                     /locus_tag="PWU81_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1109212.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            55023..55940
                     /locus_tag="PWU81_000479"
     CDS             55023..55940
                     /locus_tag="PWU81_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1109213.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            55937..56608
                     /locus_tag="PWU81_000480"
     CDS             55937..56608
                     /locus_tag="PWU81_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1109214.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(57149..57637)
                     /locus_tag="PWU81_000481"
     CDS             complement(57149..57637)
                     /locus_tag="PWU81_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HDL1109215.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(57838..58104)
                     /locus_tag="PWU81_000482"
     CDS             complement(57838..58104)
                     /locus_tag="PWU81_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL1109216.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(58104..58367)
                     /locus_tag="PWU81_000483"
     CDS             complement(58104..58367)
                     /locus_tag="PWU81_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL1109217.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            58843..59028
                     /locus_tag="PWU81_000484"
     CDS             58843..59028
                     /locus_tag="PWU81_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL1109218.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(59170..60993)
                     /locus_tag="PWU81_000485"
     CDS             complement(59170..60993)
                     /locus_tag="PWU81_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDL1109219.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(61591..64296)
                     /locus_tag="PWU81_000486"
     CDS             complement(61591..64296)
                     /locus_tag="PWU81_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDL1109220.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(64602..65549)
                     /locus_tag="PWU81_000487"
     CDS             complement(64602..65549)
                     /locus_tag="PWU81_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDL1109221.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            65739..66008
                     /gene="rpsN"
                     /locus_tag="PWU81_000488"
     CDS             65739..66008
                     /gene="rpsN"
                     /locus_tag="PWU81_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL1109222.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(66184..66546)
                     /locus_tag="PWU81_000489"
     CDS             complement(66184..66546)
                     /locus_tag="PWU81_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL1109223.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(66557..67678)
                     /locus_tag="PWU81_000490"
     CDS             complement(66557..67678)
                     /locus_tag="PWU81_000490"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL1109224.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(67694..68044)
                     /locus_tag="PWU81_000491"
     CDS             complement(67694..68044)
                     /locus_tag="PWU81_000491"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDL1109225.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(68174..69685)
                     /locus_tag="PWU81_000492"
     CDS             complement(68174..69685)
                     /locus_tag="PWU81_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL1109226.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(70007..71359)
                     /locus_tag="PWU81_000493"
     CDS             complement(70007..71359)
                     /locus_tag="PWU81_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL1109227.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(71512..72588)
                     /locus_tag="PWU81_000494"
     CDS             complement(71512..72588)
                     /locus_tag="PWU81_000494"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL1109228.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            72784..74529
                     /locus_tag="PWU81_000495"
     CDS             72784..74529
                     /locus_tag="PWU81_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDL1109229.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(74809..75246)
                     /locus_tag="PWU81_000496"
     CDS             complement(74809..75246)
                     /locus_tag="PWU81_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDL1109230.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(75230..76603)
                     /locus_tag="PWU81_000497"
     CDS             complement(75230..76603)
                     /locus_tag="PWU81_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109231.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(76925..77413)
                     /locus_tag="PWU81_000498"
     CDS             complement(76925..77413)
                     /locus_tag="PWU81_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1109232.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(77435..78037)
                     /locus_tag="PWU81_000499"
     CDS             complement(77435..78037)
                     /locus_tag="PWU81_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL1109233.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        22472 a        16581 c        14194 g        24833 t
ORIGIN      
        1 cttatatata taaacttttg aaagtcaata aaatcaaggg atttagttta tgattttata
       61 tcatttcagt aactcagaat attccgactt ttttgtacaa aaaaattaac cttacttata
      121 tgaagattct aagctcttat ttaaatttta gacgggcagt tatccgtaga agttcttaga
      181 atagctaatt tacataatta aattatatga aatttaagtt tgaacaatat actaagaact
      241 ccccttacta taaagtgaaa taaattagca tatgaataac cgtgctacca agcttttcat
      301 taataaaata tttttattaa tattttataa ctttacgcaa gatcataatt ttgtcgggag
      361 cgtcaataat tttgtgtaaa tggaaaagcc cattctgttg atcaagttaa aacattgatt
      421 ctaatgtatc atgtatttct ttaaaacctt tatgaactcg gcatagaaac ttttgattat
      481 agttgttgaa ttgagatact agaaatctct ccagagattc ttcattagga aattgctctt
      541 ttcttttggt atatttcttt aattgtttgt taaaaccttc aatcaaatta gttgaataaa
      601 tagttcttct gattgacgga gggaaattat aaaaggttaa tatagcagga ttcatgagca
      661 atttaaccac tcgtggatac tgctttttcc atttatctat cataaacctt atttgattat
      721 ttgcttcttc ttttgaactt gcttggtaaa ctaatttgaa atcgctacag atttcttgtc
      781 gatcactaac acgaacctta tgagcgatat ttctagatat atggacacaa cattgttgaa
      841 attgagcatt tggataaaca ctatgaatac tatcgtcaat accacttaaa ccatctgtta
      901 cgactaacaa aacctcttct aaaccacggt cttttagatc ttgaagaatc tctttccaaa
      961 cataagcaga ttcagttgga gctatagtaa atcctagaac ctctttagtt ccatccaacc
     1021 tgataccaat tactatatag acagcttctt ttgatacggt ttgtcttttt aagggaatat
     1081 gagtagcatc cataaaaatg actgagtatt tagcttctaa agttctttct ttaaatgccg
     1141 aaacatcttc agacacaatt ttactcatat ttgaaatagt ttgtggtttg taataatgac
     1201 catacatttt ttcgattaga tcagagattt cggacatagt aattcctttt tgaactagct
     1261 gaataatagt ggtttctaaa gagtcattgg ttcttttata agcaggtaaa gtttgttgag
     1321 aaaagtctcc attcctatct ctaggtatta ctaaattcaa ttcaccgtac tctgttttaa
     1381 aagagcgcga ataattccca ttacgggaat tacctgaatt aaatccagct ctatcatact
     1441 tttcatagtc aagaaatacc gttaattcag cttgtaataa agcgttaata gccaattcta
     1501 ggtgacgacg aaacaaatca tccaaatcac ctttattgat tagtatttcc ataatttctg
     1561 tagtaaaatc gttcatatgg aagtcctctt ttctgtgaat gtgttgtggt aactctattc
     1621 tacagaaggg acttcctttt ttctatttac acaaaatatt ttacactctc ttcaacgata
     1681 ttcttgtaag taattatgcc ccaactagga tttgtgggtt catttccatc tggagttaaa
     1741 aaaacaaaaa tttttttata gttagaataa ttgcgttcta tatatatttg gtatttatta
     1801 agttgccctc cagactccga agacagaatt ttattttcga tgcacaccac tattttattt
     1861 ttttccgata ccaataaaat atcaatattc ttattttctc tgtaaacaat taaatcttca
     1921 tagctattaa gtaaagcatc aaaataattg atagcagctt tggtatcatc attagaatct
     1981 atataccttt tcaaaaacat tttaaaaaac tcatcagata aattgtgggt ttcatttgga
     2041 gcaaacaaaa aagctaaaaa attactatgt cttatttcat ttttagatag cttaagtatc
     2101 tgaaatatat ttgttttccc agtccattta gatagttttc ttcaatatca tccatatcct
     2161 ctaatagatt ttttaaacac tttttttcat ctatcatgtt ttttctcctt gccattattc
     2221 taactaaaat tttacgtatt tgtttctatg tctacattat ctttttaaca gtatctattt
     2281 ttcttttgca acttacttgg ctaccaaagc aaataaaaaa gttatttata aatttctccc
     2341 taccactacc tattgctatt atttaaaata gggataattt tttctcttaa aaaatatcat
     2401 tatttgtggt atggagcccc cgcattgatc cggaaggcac ggtagatttg ctcgacaagg
     2461 atcagacgca tcaattgatg tggatacgtc atttttccaa aagagatctg agcattgctt
     2521 cttttcatga cggcttcgct gagtcctaaa gagccgccga taacaaaaac aaggtggctt
     2581 tttccttgga tacctaattg gtcgatttgt ttagcaaaag attcacttgt aggatttttc
     2641 ccttcaattg ctaaggcata gacgtattct tgttctttga ttttcgctaa gatccgttcc
     2701 ccttcttttt cttttacttg tctcatttgc gcttcgctta aattctccgg ggctttttcg
     2761 tcaggaactt cgatcaattc gatttttgca taggcattca gccgtttgac atactcattg
     2821 ataccttgga tcaggtattt ttcttttaat tttcctactg agataatttt gatattcatg
     2881 ttttctctcc tttggtcttc atgctcactt tgtttattat aatcaatttt ttcgtctata
     2941 tgtttatttt ggctattttt gttaaaaaga tttccccaac agttatgcac aacatccaca
     3001 ggtttatcca cagaaaacag tgttatttta ccttatttca tcacattcat taggcaaaca
     3061 tacgttttat aagaatttat ccactttata cacaggctgt ggatatgttt tatcccttat
     3121 ttttccaatc ctatccgcgt agttttacac agtatctgtg gaaaaagaaa atcaaatatt
     3181 ttcatctgaa aacaatgatt tttcctaaag tttttcataa aaagttcaca gtctcaaagt
     3241 agactaaagt caacaaataa gactatacta acagtagata aatgaatgta attggaggag
     3301 gaacacatgg atagaaaaaa tgtgacacca aagatgaaaa aaagtaaaaa taatagcctc
     3361 tggcgtaagt taggtctggg gcttgttgga gggatcgttg gaggactact gacagctggc
     3421 attttttatg cagcgatggg cactggcaac aatagttcta ctgctacaag cggcaatcag
     3481 aattctgccg gtgaaacagt tgtggaaaat gtcaaagtca atgtagattc tgatatcacc
     3541 aaagcggtag ataaagttca agacgctgtc gtttctgtta tcaacttaca aagtcaaaac
     3601 caatcaagcg gttttggcgg actattcggg cagcaagaag aaagtagcag ctcagatgat
     3661 agtaatttgg aagcttatag tgaaggaagc ggcgtgatct acaaaaaaga cgggaatact
     3721 gcttatgtag tcacgaataa tcacgtagta gatggccagc aaggtcttga agtcttgatg
     3781 aaagacggta cgaaggtaaa agctgaatta gttggaacag atgcttattc agatttagcg
     3841 gtgttgaaga tcaattcaga caaagtcgaa acagttgcct cttttggtga ctcaagtgct
     3901 ctgaaggttg gtgagcctgc gattgcgatt ggttccccac taggatctga atatgcaaat
     3961 tctgtcactt caggaatcat ttcttccttg aaccgacaag tgacaagtac gaatgaatcg
     4021 aacgaaacag tcaatatcaa tgcgatccaa acagatgcag caatcaaccc tggaaattct
     4081 ggtggtcctt tagttaatat cgaaggacaa gttatcggta tcaactcaag taagatcgca
     4141 agtacttctg aatctacttc taatgtaagc gtagaaggaa tgggatttgc tattccaagt
     4201 aacgatgtag tgaatatcat caatcaactg gaaaaagacg gaaaagtgac acgtccagct
     4261 ctaggtatca caatggtcga cttatctgcc gtttctactc agcaacaaga acaaatcttg
     4321 aagattcctt catctgtaac taacggtgtc atcgtgacaa atgtccaaac agctacaccg
     4381 gctgaaaaag ctggattgaa acaatacgac gtcattacta agatcgatga caaagaagtt
     4441 tcttctggtg tagaacttca atctgctttg tataagaaaa aagtgggaga ttctgtcaaa
     4501 atcacttact atgaaggctc gaaagaaaaa acaactactg ttgaattgac gatcgatcaa
     4561 tcggcattaa aacaaaatca aaatagtgaa aattaataaa aaagaggttg tgacaaagac
     4621 ttgtcacaac ctctttattt tggaaatgct tctccgacta cggcatcctc aggcaatact
     4681 aagattcctt tttttgctgg agcatttggc aaatgcaatt cacgagcgga acagatcatt
     4741 ccatagctat ctacccctcg caaagttcct ggccagatca tcattccatc tggcatcata
     4801 gctcctggtt ttgcaacgac aactttttgt ccagcttcga tattgggtgc accgcaaacg
     4861 atttgcaata ctgtaccatt gtctacttct gtctcagtaa tcgataaatg gtcagaatca
     4921 ggatgtggga cgcatgtttt tacgtacccg acaacgatct tcggttcatg atcagcttca
     4981 agctgttctt cgaatcctgc ttctttgatc aatcgattca atttcgctaa ttcctcatct
     5041 gttaagctta cttggccgtt cccttcgatt gttagatgat cggagatatg gaaaaagttc
     5101 caagcaacaa cttgaccttg ttcatctttg atccgagcaa cattccattt gcgttcaaca
     5161 gtgttttctg ttcctttatc gtcggctata ataaccatca aggtgtctcc gacgtgtgct
     5221 ttgttataag caaaaatcat gttctttcct ccattatcct ttgctgagat ttatcttaac
     5281 aaaaaattgc attcactgca tgaaattttt ttcatgcttt atttccgaag aagcctcttc
     5341 tggaacacca tttattcgta acaagggact gtgacaagac ttttgtcaca gtccctttac
     5401 tgttttagtt aattgattaa aaaggtcgta caagttccac ttgcttaatt acttggctgt
     5461 tcttcttcgt cagaaacgat caagacatca caaggtgaat gacgtatcac gtagctggct
     5521 acacttcctg tcataaagcg ttcgactgca tttagacctg actgcccaac catgattaga
     5581 tcgatctcat atttttttgg caatttgact gccatggatt cacgaacaga accatatgca
     5641 ataattcctt ctatatttgt gtaatcaaca gatttcccat attctttaca ttcttcgatc
     5701 atttccttgg ctgtttcagt ttgctgatcg actagatttt gatcaatagg tgcatagccc
     5761 ataaagttat aaaattgctg atcgattacg tttgctacat atactttccc attattacgg
     5821 cgagccactt ctaccgcttt tttgaaagca gcaagtgctt gttcactacc gtcgatacct
     5881 actaaaatat tcttatattc ttgattcatc ttgacccctc ctaacagatg tcagcctttt
     5941 gttacacttt ctaggaacga ttcgatttcc tgttttgttt tacgatcttt attcactaga
     6001 cgacctaatt cttttccgtc ttggatcacg acaaaacttg ggataccaaa aatgttccat
     6061 tcagcggcta catctatgta cttgtcacgg tctacttcaa taaattgcca atcagaaaag
     6121 tcattttcaa tttcaggcat ttgcggtttg ataaaacggc agtctccgca ccagccggca
     6181 gtaaagaaaa atacactctt tccagtttct acgtagcctg ctaattcttc taaactattt
     6241 gggataatca ttctttgtcc tctactttct gtttatgtat ttttcaatta aattatacac
     6301 ctttctttcg aactaacaaa cattgatcct ctttttataa aaaaaatctg gcaaaaagca
     6361 atcttttgcc agattttata gtttcactat cgcattattt tccgtatacg atcgtattta
     6421 ctgttgattt atccaaacct ttcagtgttt gaatcaacat ttctcgtgca gcttcaaaat
     6481 cgcgaatact gaacatcgtt tgatgcgtat ggatataacg tcccactacg ccaatcactg
     6541 tacttgggat tccttcgttt tgtgtatgag cagcacctgc atctgtaccg ccttttgaaa
     6601 caaagtattg ataagggata ttgtttgttt cagcgatatc cagcaagtac tcgcgcaaac
     6661 gaggaagcat gatcaaacct ggatcttgga tacgcatcaa tgtaccttct cctaaatgtc
     6721 cgaatgtgcc atttttcgtc actgtatcat ctgctgcaga gcagtctact gcaaagaaca
     6781 gatctggctt gaatttattg acagaagctt tagaaccacg caagcctact tcttcctgga
     6841 cattcgcgcc tgcaatcaat gtatgaccta atgtttcatt ttgcaacgct tctaaagctt
     6901 ctaataccat tgtacagcca tagcggttgt cccatgattt actgataata ttctttccgt
     6961 tggctgtttt gatcgtttca gcgtaaggaa cgattgtgtc tcctggaagg acaccgtaac
     7021 tcattgcctc ttctcttgat tcaaacccag catcgaacaa aatatcggtt acttctactg
     7081 atttttgtcc gcttgttcct cgcaataaat gtgggggaac ggaagaagaa atacatggat
     7141 aattcttacc agtagaggtt ttaagtgtaa aacgctgagc agaaacaacg taaggattcc
     7201 agccgcctag aggtacgact ttaaaaagcc cgttttcttg gatttgtgtc agcatgaatc
     7261 cgacttcatc catatgtgct gcgaccatta cacggggtgc atccgcttct tcatgatggc
     7321 gcagaccaaa aatgccgccc aaaccatcta actgtaactc atctaccagc ggttccatat
     7381 gactttccat ataagcgcga acatcttgtt caaaaccgct tgttccttgt aattctgtta
     7441 actctttgat tcgttgaaat gttttttctt ccatatgcta ttcgtctaaa tttcagacta
     7501 aactcctctc agacttatat tgatctgcgg ataaaatatg attttttctg cagaccgatc
     7561 gtatttatta tagcttattt tttctggtag cggttattta tttcaaagaa aatatgagag
     7621 tgtggtaaaa taagcacaag gagtaacaag tgagaagaag gaggtatgtc cacatgtatg
     7681 aagaaagcga actaagctat tttaaaggcg ggttagcagt tggaatcggt ctaggattat
     7741 tgagcggggt agcttcaacg atttggtatc atcgcaaaaa agtattgtca gcagaccaag
     7801 tacttgaaaa tatcaagtca tcttttctaa aagaaggtgc aatcgaaggt tcttggatct
     7861 cttttgaaaa ggaacctatt aggaaattcg ccgttcggtc gagtgcttat cggggaggtc
     7921 tttctcgaat agaagatggc gaaactgttt attatgaatt tttagcagat gctcatacag
     7981 gaaccgtatt agaaatttca agaaaaaaag gtgcaacaga ttaatacgtc aaaaaagtga
     8041 gagaacgaat acgcacccac tattcaaaag agaggtatca tgtccttctt ttgtagtggg
     8101 tggtttttta gatagagctt tccaaacata tattccagta ttttcttttt aaataaatga
     8161 tatttagcgg gaaaaactag catttccttt gattatgagg tatttttaat ttagcttttg
     8221 ttttctatag ttaaggagag tgaaaaatgg aacaacaaga aaaaattgct gttctgattc
     8281 cttgttacaa tgaggaagca acaatttcaa cagtcattgc agactttaaa cgagaattac
     8341 cagaagcaga tatttatgtc tatgataata attcgaccga tcgaacatac gaattggccg
     8401 ttcaaggtgg agcaatcgta aaaaaagaac ctcgtcaagg aaaaggaaat gtgatccgcc
     8461 aaatgttctt tgatatcgat gccgactatt atttaatggt agatggcgat gatacttatc
     8521 ctgcagaagc tgtacatggt ttgctcgaga aattacgttc tggtgaagca gatatggtca
     8581 ttggtgatcg cctatcaaat ggaacctact ttgatgagaa taagagacct ttccacgatt
     8641 ttggaaataa ccttgttaga aatacaatta ctagaatgta taaaacaaaa attcttgatg
     8701 tcatgaccgg ctatcgagga ttcaatcgta tctttgtcaa aagttttcct attatgagtt
     8761 ctggttttca aatcgaaacg gaattgacga tccatgcttt agataaaaaa ttcaagttcg
     8821 ttgaaatccc aatcgattat cgtgatcgtc ctgaaggcag cgaatcaaaa ttgaatacat
     8881 tctctgacgg attcaaagtg atcatgatga ttattaaaat gtttaaagat tataagcctt
     8941 tgcttttctt tgggatttgg accatcttct tctttttatt tggattggtc acggggattc
     9001 ctgtgattcg cgaatatatg ctgacaagct ttattacacg catcccttct gcgattttaa
     9061 gtactggatt gatgacttta gcacttcttt cattagtaac agggttgatt ttggatacag
     9121 tcgtcacgaa cgctaagaaa gaatatgaat tgaatctata tcatgtttat catgaatacc
     9181 acagtagaaa gtaaaaaaag gatcaagact gcaaaaataa tttgcaattc ttgatcctct
     9241 ttttaattaa ttatttcgcc aaacgataga tggcatctgc atagattgct gttgctctga
     9301 acaagtcatc aagactcata aattcgtttg cttgatgcat tgtatcagta tatcctggga
     9361 acatcgctcc ataagcaacg ccacgtttca acaaacgtcc gtaagttccg ccaccgatga
     9421 tttgttcgta gcctttttct ccagtgtggt cttcatagac ttgcaataat gtagccacca
     9481 atggatcatc cattggtacg taatgtggtt ccattacgcg agcacctcga gtaactgttg
     9541 cgccttcttg ccccactgtt tgttcgatgt cactttgcaa tccgtctact gttacacctt
     9601 ttggaaaacg gaaattcata ttgatgaagt tcgcttcctc tgtaccattt tcttcaaaag
     9661 caaatagtcc agcattcatt gtcaatttgc ccattttttc atcttcataa gcgactccta
     9721 gtttttctcc ataaaaatct tcatggacat aagcagctgc tacatggata aattgtttag
     9781 cagatcctag gaattcatag ttatctaaga acaatgccaa gaaagaacct gcattgatac
     9841 cagattgtgg gcttgcacca tgtgcgcctt taccaactaa ttcgattttt acatacgtat
     9901 tgtccgcttc aatcgttccg cttactggat ttgcttcgat gaattggtaa aatgcttctt
     9961 ccatcgcgat agctgcatct gcacttggta cttgaagtgc tgcagttgct gttcctggca
    10021 ccatattttc gcgaagtccg gaaacaaaag atttcaagac ataatctcct gcattgtcgc
    10081 ctttgaagcg taaagctaaa gaaacatttc ctttttcacc attgatgatt gggaattcag
    10141 catctggtga gaaaccaaaa tctggttctt cttcatgttc gaaataataa gccatatcgc
    10201 cccagccgct ttcttcatcg ctgccgacta cgaagcgaac tttttttgat agtttcaagt
    10261 caagttcttt aatgatcttc atcgcatagt aagctgccat acttggacct ttgtcatcgc
    10321 ttgatccgcg tgcaaagatc tttccatcct tgattactgg ttcataagga tctgtgtccc
    10381 agccatctcc tgcaggaacg acatccatgt gtccaaatat ccctagcgtt tcgtctcctt
    10441 cgccaagatc aatatgccct gcatagttat cgacattttt tactttaaat ccatcacgtt
    10501 cgccataagc taacatatgt ttcaatgcgt cgcgaggtcc aggtccaaat ggtgcatctg
    10561 gtgttacttt tgagtcatcg cgctcgctgt tgacacgtaa aagatttttc aagtcttcaa
    10621 gcaagtcttc tttacgtgct tctacttctt tttgccaatc aattgtcata aagttaatcc
    10681 tccacttatt taagtcttcg tgttcatgat accacaaatt acagaaaaaa atagttcaaa
    10741 aaatctgaca attccttttc ttatctgttt ttctctaaaa aaaatatacg ctataatgag
    10801 taaaaggagg tttttagatg ctgataaacg aacaactagt gaaaagagtc atcacacctc
    10861 gtccaaaaat ttcacataaa ggaacatttg gacgtatcgt tttgattggc ggcaatgaac
    10921 aatatggcgg agcaatcatt atgagtgctt tagctgccgt acacagcgga gcgggtctca
    10981 cgactgtagt aactgatgaa aaaaatcgga gtgctcttca cagtcatctg ccagaagcaa
    11041 tgattgtagt ttggacggat actgaccagt ttctttccgt aataaagcaa gcagatgtac
    11101 tcttgattgg tcccggattg ggtacttctt ctaagagttt ggaacgtttc aaacaagtac
    11161 tagaaaacca atcaaaagaa caatggctgg tgattgatgg ctctgcttta agtttacttg
    11221 ctgctaatca tcttttgcta ccctatcccg aacaaactgt acttacacct catcaaatgg
    11281 agtggcaacg gttgagttct ctaccgatac aagaacaacg ggataaagaa aacaaaaaga
    11341 agcaacagga aatcggtgct gtgctcgttt tgaaaagtca tgagacaaaa atttatggtc
    11401 accagcactt ctacaccaat cctttaggtt ctgctgctat ggcaacgggc ggcatgggtg
    11461 acactttagc tgggatgatc acaggatttt tagcccagtt tcctcaaaat gaccaaacga
    11521 ttgcagcagc tatttatctt catagtttca ttggagacca gctggcaaaa gaacaatata
    11581 ttgttttgcc gacacaaatc agcgaagcac ttccgaaatg gatgaattat ttttgtaaaa
    11641 aatcaatgga aaacgaggca tgagtcatgg agaaatttac aggttatgtg cgagaacctt
    11701 tttattatga aacagatcaa atggggatta ttcatcacag caattatatc cgctggtttg
    11761 aagaggctcg ggtagcctta ttggtacatc tagggtttcc ctataaaaaa atcgaagaat
    11821 ctgggatcat catccccgtt ctggaagtaa attgcagcta taaagaaatg atccattacg
    11881 gagaaaaagt gagcatccat ccaactatcc aaaagtatac ggggacacgg ttggatttta
    11941 gctacgaaat tgtgggaact gaagacaaaa aattaaaaac tactggcagt agtaaacact
    12001 gttttttact ttctgattcg caacgattag tgaagctgag caaagtcaat ccggagttag
    12061 atcagttatt ccaagcgtac gcttctttgg atgatgtaaa ttcttaataa agaccgtcta
    12121 agcatttttc ttgtattgtg tcgagtagtt cgtataatga aaagaaaaaa gcaggtgatc
    12181 cccatgtatg tcgttaaagt aatgcacggt tacatcgata aaactggctg tcgcactcgg
    12241 gaaaaaaact tagacaatct gctcattttc aaagacaaaa aagaatccga agcttttgct
    12301 aaaagaatcg gcggtcgtgt caaaccgatc caagaggtac gaccagattg attcaatatt
    12361 tttcttttcg aggctctaat aggcatgcct atcagggctt tttttgcaga ctgtgttttt
    12421 atgcccacac gatttgtaag cgatttatgt tataatagat tatatctgca tagaaaggaa
    12481 ggttaaatgg ttatcaataa tttgcaacag caagcttatg aatctatacg taaacaaatc
    12541 atttattccg agttagcacc tggaagtaaa atctccgata aagtgttaga agaacaacta
    12601 gctatcgggc gtacaccgat tcgagaagca ctgatccaac tgcgcaatca agagcttgta
    12661 aaaaccattc cacagtccgg cacttatgtt tcaaaaatcg atatccgatc tgcttcatta
    12721 gcccgttata cgcgggaaaa gttggaaaaa ccgatcctgc aagaatgcag tgcgaaaatg
    12781 accaagcaag atcaagctaa actagaaaaa atactagaac aaaccgatca ggcagtaatc
    12841 gctcaaaata aaaaagcttt ttttgatttg gatactgatt ttcaccgaac ttgttatgaa
    12901 attgctggaa aacgagaaat ttgggattgg ctagaaagtt atagtacgcg tttcagatgg
    12961 ttacgtttga cgatttcaga attagactgg ggtcgagtat tagatgaaca tcagacaatg
    13021 cttcaatcaa tgattgatca taactttgat gaagtcggat ttctttgcac gatgcattta
    13081 catatgatta tcgaggagca agaatacgtg attcataatt accccgatta ttttgaagat
    13141 attcaggaca gacccatcaa ataagaggtt ttggaatcag cgttttgacc tgagtattag
    13201 agaaagaaac ggcaaaatag ttcctcatat tttgccgttt tttttgagct aataccgagg
    13261 gtcagctgat tgcacaccgt ttattgagag gttgtgaaaa agctgccctg catcaagtaa
    13321 taagaacagc caagtaaaaa tagcttctta tgtttttaac ttggctggac ttattaccgc
    13381 agatgcaagc ttttgaacac cgttttccaa gaggtcgtga gcctgccgtt catctccgag
    13441 tcacaaggaa caatttcaaa aaacagcttc tcatgtttgt tgaatatttt gacttgtgac
    13501 cgaggagttg acaggagaac atcgtttatt cggaaaaaaa ggactgtgac acgacttttg
    13561 tcacagtctc tttatttgta atctaaatca tactccgtac atcttttttc tatttttttt
    13621 atttcttcta agatcccttg gctacgaatt tcattatatg gccaaagtaa gactgcctct
    13681 aatacataag gcgaaaaatc gctttgattc atctcttttg aaaaactcat cgctgctatc
    13741 tcgctcaatg cacgaatgtt tgattgatgt gtataaaaag gcaaccgcca cagcacaatt
    13801 ttttcgcttt gcgtataaat tcccttaaca gaagcttcga tgtggtgaaa tagttcatgt
    13861 ccaataatca aagcttgcgc tgtttcttta gagatcgaag ctagtcctga acattttaat
    13921 ttttctaacg gctccgtcat caatcgaatc ccttcatctt ctgcataagt tgcaaaagtg
    13981 attcgttcgt ttatttgctc tgattcacag gaaacgattg gaacagccat ttgttcacaa
    14041 atttctccta catcggcaaa agggtacttt tttttgatgc tacatgccgt ttcgatacca
    14101 caatcgattg attgttgtat aagaattttc tgcttggaca aaggaatctt tttccaaaat
    14161 aaatctcgcc gaaactggta gaatccccaa atttcttcgg ggatctgttg taattcgttt
    14221 agtaactctt ctaacttatg tttgtttgat atttccggca acgacgatca cttcttcctc
    14281 cggttccata gtagatactt ccagatccat caaaagctgt agttgttcca gatccgtcgc
    14341 tgtaaaatca gagactcttg ctcctaagca gagataaaat tctggtcggg caatcaattc
    14401 agtttgataa actgccatat cttcccaaag ttgttcgaca gctgtcatat aattggctgc
    14461 agttgttttc atacatgaag catgtccacg ttggaccttg ataaatcctt tttgatcgat
    14521 gatccgaaga cttccagcat tttttgaacg attctgttcg ccgcatacat aaaagaaagg
    14581 tgtattttcc aataagcaaa cttcattttt atttaatcgc atatcctctg cagcaagctc
    14641 tagtgcttct tctgttgtga tctccttggt tagatcggtt gctttcactt ccgtagaacc
    14701 agtagcgata gccgttactt ttgaagtctg tggatcgatt tctacgtgga cttcgatcga
    14761 ttctggtgta gcgcctgatt caatcgcttt attcatcgct tcatttttca acgatcgtat
    14821 atcttcttta cttggcgaag ggatgatacg ttctacaaca tctcggacca ttgcaagtgc
    14881 tacaccaata gaagaaatca cttcggcgtt ttcaggaatc gaatatttca cacccatttt
    14941 attactaaag taagtaatca atgaggcagc gccgccccct acacctacaa gagaaatctg
    15001 atctttctcc aagtgatact tgtctgccaa ttctaaaatc accggttcaa tcttagcata
    15061 ggatttttcc atgatctgtt ctgcgatgtc ttcaaccgtc gtatgacaat aatcagctaa
    15121 tgcttggatg gcttttcgtg cagatgggac atttccataa gaaaaatgtt cttcttgcac
    15181 taatccaagg acattggctg cacatgtatt tgtaatagtc acttcttctc cgtcttccat
    15241 cactaccttg acgtaatcag cagggtctcc ttctttagga gagaaaaatt caaccttagg
    15301 acctttgatt ttttctgtct ccgtaaatac tgcataatct agaccggcaa tatgtgcaga
    15361 acgtggaccg acatcgatga ttcctgactg attagcccgt accatcgaac cgcctgccac
    15421 acctagaacc cgaacatcta atgaagagat atatgttggg tggccaccta caattgaata
    15481 atcgattgct ggacgtccat ttttaatgac cccgatattg gttgttgttc cgcctacttc
    15541 aaaataaaca ccattagacg ctctcaagta catcaaagag cccatgactg atgctgctgg
    15601 accggaaagc atggttaaaa caggtcgttt tttcatttca ttgatttcca ttactccgcc
    15661 gtctccgcgc ataatcatca aagaaacatt gacccctgct tctcgtacgg aatcttctgt
    15721 agaggttgcc gtatctagca tttttggtaa aatagatgca ttgattgctg ctgtccgagt
    15781 tcttcttgtc aatccatata attttgtgat gtcagaagcc atcgttgtag ggatcgattt
    15841 taccgatgct gcttcataaa caactcgttc tggttcccca ttatctactc caaatgccat
    15901 agaagaaacc aagacctccg ctctttctct ttctaaagaa gaaatcgttt ctgaaacacg
    15961 gtctacattt aagtgtttga caggtaagaa tgcattgaca atctctattt ttttcttatt
    16021 ccctaaatcg atatcgttca atctagtttg tcgttttgct aaaaaacctt ctaatccacc
    16081 ttttgccatc ccgattacgc cgacttttgc tacgtctcct tcaatcagtg cattagtcgc
    16141 ttgtgtggta gaatgtgcaa caaacacaac atcttctgga ctgatattat tttccagcaa
    16201 acaattttga aaagactgaa cgacacctgc ggctactcca cggacatcat cgtgggttgt
    16261 tttcactgaa gattttccga tgatttcgtg cgtcgcatta tcgattgcga ctgctttcgt
    16321 atgtgtaccg ccaacatcaa ttcccattcg cacaaaacgt ttcattttct tttcctccat
    16381 tcatctaatc gtttttatcg acgttttgtt tactaggcgt tcccgtacaa taagaacgtg
    16441 atcaggcata agatcgctcc gatgatccaa ccagttggga tggatttctt catatattct
    16501 cgagaagtta cttttgtata acctaatccc caagcaaccc aagattgtgt aatatctaaa
    16561 tgctgaggag caatcgttgt gattgcaaat aacgggaaca aaaaggctgg cgacatcgtt
    16621 ggattaacag ctaatacgac agctaaaata gctgagcctg aaccgactag attcattgga
    16681 ccgcggaaaa atcctagtgg tgtcaatata gcaaatacga ttgccaagac taatggtgat
    16741 tgcggtatcc aatctccaaa aatagctgaa aaataaggag aagcatacgc agctgaatta
    16801 ttgaacatcg ctaaggtcat caagaaacca accattggcg caacatctac tgcgccatct
    16861 gtaaataatt tcgcaaactg acgacagatt tctttataac ctccgcgcaa ttttccagta
    16921 aacagcaatg cccagatacc tgctagaata aaacctaaga tgattggaag atctaaaaga
    16981 acgaccccta aaactggtaa taatacggct aaccatgaga tcattggtgc gtcgccacct
    17041 tcagctggta cgtttgctgc cattgcatag cgtttctttt tgttcattga tatattagcg
    17101 accgttagta ccacaactaa gcttacaatc atcccaatca taccaatttg gaaatagtca
    17161 ttataagtat agctttcagc cgctggattg aaaccagcat aaatcgtttg atattgtttg
    17221 aaattgacga tattagcaaa gattccagcc ataatcgagc ccataaatga gaacagtgca
    17281 accggtactg aaatacctaa tgacatcaag attggcaaca caataactgc aatagaaatc
    17341 actggtccga ttcctgtcat cgacatgaat ataacagcgg ttacaataca tagtaagctc
    17401 atcgtgattc ttggcttgtc ccctccaagt tcaactacct ttcgaatcag ggtagctgcg
    17461 attccgctat ctactagaac acgcccgaaa aaggcaccaa agaagacatt taccaagatc
    17521 gatttggcgt actcagctgg ccccgtcgta tagatatgcg tcaacacatc aattactcct
    17581 tgcccttcca tcgccgggtt tggtgaaaac gtgtttccga ctagcgctaa tcccatccat
    17641 acagtagcca tgatggcaaa gccgaccatc aagttgtagc cttttacaca gtaccagacc
    17701 atgaaaaaga atgagataac taacaaaata ccaataataa tatcactcat gttattcctc
    17761 cgtccatttt gtttttctga taagcaaact aatatatcag tcaaactcat tctaacttat
    17821 gtagttagcg ctttcattaa agggtgacca taaatgttca gttttcaatt aaaaaacctt
    17881 aattatgcta ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa
    17941 taaaaaaaga ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag
    18001 cttatttagc caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt
    18061 taacagccaa gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt
    18121 gcattaattt tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc
    18181 acaatttacg gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat
    18241 tcattacttg ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg
    18301 ctaattttag cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca
    18361 tgtataattc ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc
    18421 atttttgcta gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta
    18481 cgcaattgac ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg
    18541 aagcgtttca atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt
    18601 ttttgttttg gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg
    18661 tccaacagcc agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga
    18721 acatgctgcg tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt
    18781 cagctaagcg attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga
    18841 aacggataga agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt
    18901 ttgtattgaa gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca
    18961 ctttttgttt tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt
    19021 agtccatgat tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt
    19081 ttgcttgttc tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat
    19141 ctgaaccgat caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg
    19201 ctatggtcat tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa
    19261 taaaaaaacg agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa
    19321 ctatctagcc cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc
    19381 tcaaccatca tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc
    19441 ttccttttct gaaaatgatt agattagcag actttctagc agtgattccc gcttctttgc
    19501 tatactaaag aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc
    19561 ttcctcttct tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc
    19621 tgatcaacgg gggtgaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc
    19681 atatccaaaa acttacgatg caaaaaacag ttttacgtca ttttgaatgc agtaatgtca
    19741 tttttgaaaa atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg
    19801 tgtttcatca atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta
    19861 ctttcactga ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct
    19921 ccttcgatca ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt
    19981 ttttgtctga caatcagttg accggttcga actggtttcg aacttctctg aagggattag
    20041 actttacgac taatttcttc acagcaatcg ctttatctca agattctttg gcgggactaa
    20101 tcgtcaacca agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact
    20161 agatcaacga atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat
    20221 gatcaaggcg attccccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc
    20281 gaagaatcct gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt
    20341 catcgtgtca ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa
    20401 aatctgcgaa gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gcgcatcgtc
    20461 caactcttct ttttcctttt ctgacaactt gcgataaata gccaatgcct tgatttgttc
    20521 taaaagtact acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc
    20581 tgaaagttgc agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg
    20641 acgttcacta ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt
    20701 acgaaacaat aaactgtata cactcatgtc tggcattcgt tccaaaagcg atcgaattag
    20761 tcgaatcacg tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat
    20821 aaaagcgata ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga
    20881 tacaagcagt aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc
    20941 tagagaataa gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc
    21001 tagcacatcg ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt
    21061 ttcccgcata ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa
    21121 taaaggttta ccctttattt gagcaactgt aaatcaaatg gctactttgg tttggcgaat
    21181 ctctgctaat ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac
    21241 aaaattatag cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca
    21301 ttgatcgtaa cgtctgttca attctttgta ataatcgtat agacttggat caaaggacaa
    21361 ttgttcatac gaacgaccac gtttttcgat acgctccaac atcgtatcaa aagatacacg
    21421 aatatgaact aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat
    21481 gttttccagc agttcatcat acacacgaac ttctgtttct gttgctcttc ctagatctgc
    21541 atttagatgg aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc
    21601 tttcattgct tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa
    21661 agcatactgt tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc
    21721 ataaaatgcc tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc
    21781 accaattgta cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa
    21841 tattgtgttt aactccaacc tttttaaata tacccccaat atctagtagt gtcaattgta
    21901 tttttttcta gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc
    21961 tttagaaaac gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat
    22021 gttttttgag acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg
    22081 ctttattttt tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat
    22141 ggccaaggag aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga
    22201 gaaccagcga ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg
    22261 gatgcaatgg acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct
    22321 catgcattaa aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa
    22381 aaaaacttgc aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc
    22441 gctgcagtcg ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct
    22501 cgtgaaatac tattagaaaa tgtccagctt tcggaaaaaa tcgcccatgg taatcctagt
    22561 ggaatcgatg cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct
    22621 ttcgactact tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa
    22681 ggacaaacaa gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa
    22741 actggacaaa tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt
    22801 gaaaattcac cagaaacgtt ggcacagaca atggatgaat cacaatcact tctggaaaag
    22861 ctgacaatca gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca
    22921 ttgggtgcca aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca
    22981 aaaacaaaag cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg
    23041 atacaaggat taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat
    23101 tgctctaatc aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag
    23161 cctttcgtta acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta
    23221 tatggtagat gaattttatc tagatggcac cttgcaagac gaaaaagcaa caaaaaaagt
    23281 cagtcagttt cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat
    23341 cagtcagaat ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc
    23401 tttagcaggc gcttgcaata ctgctcttaa gcttggatta gatgatctct ctctttcaag
    23461 atttgctcga cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgtcgaatg
    23521 ggaaaaaggc catgacgact taagttctta cgctaagcca gtcccttccg attctttcga
    23581 agacgattta gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag
    23641 aaatgggatg cgtcggacag tcgaaacatc cagtttttat caaggctggt tagattccgt
    23701 tgaaggggat ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg
    23761 agaaacgatg gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc
    23821 attcacttac tggtctcctg acagcttaaa agcgatggat gcggtgcgac aattgagaaa
    23881 gcaaggcatt ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga
    23941 aaacagccat ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt
    24001 aattactgca catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat
    24061 tgaagtatct gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg
    24121 ccatccagca gttatcgctg cagtcgatca attcgtgaca gtcactgtag aatctgcacg
    24181 aaaagtcgga agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg
    24241 caacggagaa ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat
    24301 tcgcttgact gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa
    24361 agtaacaagt gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc
    24421 cgtgaccgtt gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcagtt
    24481 ggagattttc aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg
    24541 cgacatcgct gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg
    24601 gctccaagaa caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg
    24661 tctatccatt gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg
    24721 tagccctgcc tctacttctg atttggtcga tcaagttcac cgttcgagag aagataaaat
    24781 ggtggcttat cagcttttct taaaaaatag tacagaatgt gtcaacgaaa tgatcaaagg
    24841 gtttaaagaa aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact
    24901 gcatgattta tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg
    24961 taatttagct gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg ggggcgattg
    25021 cggaatcgta attgttgacc agaaatctgg cattcttcct ttaatgagtg catgggaaaa
    25081 agcagaaatc actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg
    25141 aatcgaaaag acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa
    25201 tttgacttcg tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat
    25261 ctaagtacac aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact
    25321 ggcggaagcg aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca
    25381 gatttgatga ttgccactgg ttcagtcagc gctgccttga aagtcccttc tttagccgat
    25441 acttatacga ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca
    25501 gggacttctg tcgaaagagc caaagaagct atccgattgt ttcacgcaga tgccttacag
    25561 attcatttga atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat
    25621 tggaaagtac ttattcaaga aactcaaaca gccattgacg tccccctcat cgtaaaagaa
    25681 gtcggttttg gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca
    25741 gttgatatca gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc
    25801 aagcgggaac tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa
    25861 gcaaatgaag cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat
    25921 gacatattta aagccctttg tctcggcgca aatgccgtgg gtacttcagg aaccgtactg
    25981 acgcatctga tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa
    26041 gagcttcgtc tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa
    26101 tctctgatct tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg
    26161 aagtatggaa gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttgtctcac
    26221 gctctaaaca cgtacaaacg gcggaagtaa aagcaactcc ttcggaaata agctgaaatt
    26281 cacaaaaatt tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag ttaaatgctt
    26341 ctgtcccagc ctcttttttc gaataaatga tgtttaatca gctgtccttc ggtattcgtt
    26401 caatccgctg atttcacaac ctctccacta aacagtgtac taaagctctt ttaaggttgt
    26461 tttccgatat aggcaagtat gcctccgtcc acatacaaga catggccgtt aacgaaatca
    26521 gatgcgctag aagccaagaa gacagatggt ccagccaaat cgattggctc gccccagcgt
    26581 gctgctggcg tacgtccaac gataaattga tcgaatggat gacgttcacc gttttcttgt
    26641 gtttcgcgta atggagcagt ttgcggtgta gcaatataac ctggtccaat tccattacat
    26701 tggatattgt attgaccgta ttcagaagcg atgtttttcg tcagcatttt taatccgcct
    26761 tttgccgcag cataagcact gaccgtttcg cgtcctaatt cactcatcat ggagcagata
    26821 ttgatgattt taccgccgcc tttttcgatc atatccggaa taactgcttt tgaaacgatg
    26881 aatggggcat ttaaatccac gtcgattact tggcggaagt ctgccgcatc catttcgatc
    26941 atcggtgtcc gtttgatgat ccctgcattg ttgactagaa tatcaactga acctacatct
    27001 tctttgatct gttgaaccat cgcttgaacc gcttcttcat cggtcacatc gcagacataa
    27061 ccttttgcat cgatgcctgc ttctttataa tgcgctatac cttcatcaac tgattcttga
    27121 ttcaagttat taaatacgat ttttgctcct gctgtagcta aagagcgtgc aatctcaaat
    27181 ccaataccat agacagcacc ggtgactaat gctacttttc catctaaacg aaacatatcc
    27241 atactaaatt ccatcatttt ctctccctta tgcttatttt aattgatcca tttgcaccat
    27301 atccatgtct gtataggtga tattttctcc gcacattgcc cagatgaatg agtaattgct
    27361 tgtacctaca cctgaatgga tcgaccagct tggcgaaata atggcttgtt cattgtctac
    27421 aactaaatgt tttgtttcat ctggtttgcc catcatgtgg aacacacgtg tatcttcatt
    27481 ggcataatca aagtagacat aagcttccat acgacgttca tgtgtatgac aaggcatcgt
    27541 attccaagcg cttcctggtt ctaaaatcgt ataacccatt tgtaattgac agctttcgca
    27601 tacatttgga tggatgtatt ggtagatttt acgttcattc agtgtcaggc cttcaccagt
    27661 ctccattggt tttacttggt cgatactgat ttttacattt ggatatttat gatgagctgg
    27721 aacagaacta atatagaatt tagctgggtt ttcagcatct tgagaagaaa atacaacatg
    27781 tcgtgtttct tttccgatat agtatccgtc ttgttttttc atttcttctt tttgcccatc
    27841 gatctcgatg aatcctggtc caccgatatt gatcacacct agttcacgac gctctaagaa
    27901 gtaatcaaca cctagttctt tgtctaatgt gatttctaat tctttttcag taggtgtcac
    27961 tccaccaaag atcattcgat cgttgtgcgt ataagtcaaa ctgattttcc ctggttcaaa
    28021 taccttttct actaaaaatt cttttctcaa ttgttccgtt gaataatgac ggatatcttc
    28081 tggactgtgt gtataacgtg tttccatttc ttgcattttc ttctactcct tctatcgaat
    28141 gatttttcca gagccgcatg ctaaaaattg tgcgacttct tcttgactaa attggttgca
    28201 gtccccatgg atcgtatgct tcaaggcaga agctgccgta gcaaaatcaa tgatttcttg
    28261 cggagacata ttggataaaa tcccatggag cacgccacct gcaaaagcat ctccgccacc
    28321 tactcgatcc acgattgggg tgattctatg gacttgggat tccacatatt catggttcat
    28381 ccacaatgtc cccttcaatt cattttcact tgctgaatga acggtacgct tggtcgaata
    28441 aaacacctga atattcggga acttttcctg cattttttga taataataaa ttagaggttg
    28501 ttcttctgct tctggcgctt tctcgatacc taacaaataa agagcatcca tttttccagc
    28561 tgagcagata tctactaaag gtaaaagctg atggattact tcgctggctt cttgctgtgt
    28621 ccataatttt ccacgatagt tgatatcgaa gctgatcagg cagccagctt tttttgcagc
    28681 ttctatcacc tgtttcgtca ttttttgcca ttttttagac aaagctgggg tgattcctga
    28741 aacatgaaat atatctattc cttggaataa ctcctctagc gaccattcca aatgatccat
    28801 ttcagcaaaa cttgatccgg cacgatcata gatcactgag gcggcgcgtt cgccactacc
    28861 agtttccata taataagtac ctagtcttga tccaccagat aatacctgtt tcgtatctac
    28921 tccataacat tgcaagtgct ttttgactgc ttttcccaat gcgttatcag gtacttttgt
    28981 agcaaaaatc gcttcatgtc cgtaattagc taaagaaatt gctacgtttg cttcccctcc
    29041 gccataatgg gcagcgaatc gttcacttga atcgatacgg gttccctgct ctgtggacaa
    29101 tcgcaacatg atttctccga gtgtcactac ttttcccata agcagctacc tcttgatttc
    29161 tttcattttt gctgcatact gttgcgcgac ttccgttact ttgtcaaaat ctcctgtagc
    29221 tgcgggtgct aaaaggttcc cacctactcc gactgttacg acaccagcat cgaaccactc
    29281 tttcatattg tccagactca caccgccagt tggcatgata ttgacttgag gcatcggcgc
    29341 cttgaaggca gaaatgatac ttggtccata agcgcttcca ggaaatagtt tgacgatatc
    29401 taccccgctt ttcagcgctg ttttgatctc ggtaatagtc atacatcctg gtaagtaagg
    29461 aatttgatac agattgcaca tctctgccgt ttcttgatca aaggaagggc taacaatata
    29521 ctctgcgcca gctaagatag ccaagcgagc tgtgacagcg tctaatactg tgcctgcgcc
    29581 aatcactatt tctggttggt ctttgtagaa tgacaaaagt tctttgatta cttgatctgc
    29641 ttgagggact gtaaaagtca actcaatacc agttagacca cctttgatga ttgcgtggca
    29701 agcattcaaa gcttcttctt tgctttttcc gcgtaccacc gcaatcactc cggcgttttt
    29761 caatcgagat aaaatatcaa tttttttcat cttctccacc tatttcctat ttagaaattt
    29821 tattttataa cttggaaatc tatacccata ttatacggca gaaaagaaaa attgcaaacg
    29881 cttttttcat tatttttgaa ttctttgctc gatttcgctt tttgtttgct tgagggcact
    29941 gattatttgt tcttttgtct catctgttaa acgatatttc ggcatactta cactaaatgc
    30001 accaatgatt tgttcgtttt tcacgattga ggctcctata caaaaaatat cacgttcgat
    30061 ttcttcatcg tcaaatgcga tgtgggtttc ttttaccgtt ttgatttcct ttttcaaccg
    30121 taatgcattg gtgatcgtat attctgtgta agggatcaac ggatgctttt caagataatc
    30181 agtgtactgc tcttcactga attctgctaa tacggctttg cccatcgcag aattgtataa
    30241 tggtcgggtg attccgattt ttgaagacat gcggatcgtt tgatgttttg gatctagttt
    30301 gttcacgtac aagatctcat tgttgtccaa tacccctaag tggatagtct catcgatctt
    30361 ttgctgcagc tgttctaaaa acggtaaagt caactctacc aaatcgattt gttcaatgtt
    30421 tttattcgcg tagcgaataa gctttgcacc taaacgatag tttttctctg aatttttatt
    30481 tacgtagccg atcatcaaca aagtatctag tattttcaag actgtagaag aggtcattcc
    30541 cacacccttt gcgatcgtct gcaaagagac atcttggttt tccgctaaat agtctagtat
    30601 tttagaggct ttgatcaata cagttccata aggcttttta ttttccatat aatattctcc
    30661 tatttcttta taagaaaaat tatagcggaa ataccatcaa tttaaaagag atatccagat
    30721 ataaactacc atttctcttt acaggcaatt taatgttgaa gatccgcttc gtttttacat
    30781 caaactttga tataaagcaa tgatttcttc tactgtcgtt tctcttggat ttcctggtgt
    30841 gcatacatct cgcaaagcat cttctgcaat tgctggaata tctgcttctt ttacacctaa
    30901 ttcagaaatg gtttctggga ttccaaccgc tttgctcaac cgatcgatct caccacaagc
    30961 cgcttctctt acatcttcta aagacatttc agctgctcct ttaatcccta gaacaagagc
    31021 gatctcacgg tacttttctc cagtatattc tttgttatat ttcatgacag ttggcaaaag
    31081 tgctgcacac gctacgccat gaggaatgtt gtaccatgca cttaacgggt gagccatccc
    31141 gtgtaccagt cctaaaccga catttgagaa gcccattccc gcaatatact gtcctaatgc
    31201 cattttttct cgcccatttt gatcgccatc tacagaagct tccaaagaat ggccgatgat
    31261 ttcgattgct tttaaatgca gcatatcagt catttcccaa gcgccttttg tgataaaccc
    31321 ttcaatagca tgcgtcatag catccatacc tgttgatgca gccaattttt tcggcattcc
    31381 catcatcata tcgctgtcga tgaacgcgac gattgggata tcatgaggat caacacaaac
    31441 aaattttcgg tgatttactt catctgtgat gacgtagttg atcgttactt ctgctgctgt
    31501 tccagaagtc gttggtatag ctaatatagg taaacatgga tttttcgtat cagcaacacc
    31561 ttctaaacta atgacgtctg aaaactctgg attcgtcaaa ataatcccaa tggcttttgc
    31621 cgtatcgatt ggcgaacctc caccgattgc gatcaaacag tctgcctctg cctctttaac
    31681 aaaagccact ccatctttta cattttggat cgttggatta ggtacgatcc cgtcgtataa
    31741 agcataagcg attgctgctt tatctagaag ttctgttact tttgttgcta taccgtgctc
    31801 gatcaatcct ttgtcagtaa tgacagctgc tttgccgaat cctcgctgct gaaattcagg
    31861 gacgatcgac tcaatcgctc cttttccaaa atacgatgtt tcatttaaaa tcattcgatt
    31921 actcattttc tattcctcct aatttgtatt atttttcaca ataaaaacga aagaacccca
    31981 agcaaccggt tgcccagttg cttgttgcta cagttcaaag actggcatca attcccaact
    32041 gactggagag gcatctggat ttgtctccat gatatcttcc atgtacttcc accatttttg
    32101 attgatagat gtatttgcca ttttttccca taacttttca tcttcgatca ctaagtaacc
    32161 aaataacaaa cttgtactcg gatcaagata gatcgaatag tcgatgaccc cgtgttcacg
    32221 aagtgctgta cgcatttctg gccaaagttc atcatgtctt tttttgtatt cttcttgttt
    32281 ttctgggtaa actttcatgc aaaaagcttt tttcaccatg tcagacacct gctttcgtat
    32341 gaacttcctc caaataacct gcttttggag tgacaccaaa cgcatctgct aaatgccaca
    32401 ggttttcgtc cgtgattgtt tgcaagattg gtccttgcgc tttgacgtac gtatagactt
    32461 ctgctgcctt ttctgctgtc tcgatcaaac caaagacttc atccatgtct tttcctgacc
    32521 cgtagattcc atgttgtggc cataaaacca atcgagtttc tttcattttt tcagctgttg
    32581 cttccccaat ctcatttgtt cccggaacca tccacggaat gatggaaatt ccttctggaa
    32641 aaacaactaa gcattccgta cacatttgcc atagcgtacg actaaaggaa cgttcgtcta
    32701 attcatgcgt aaaactcatc gctaatagat gactagcatg gcaatgcata acgatccgat
    32761 tatctgggtc tacttctaaa cgggcgatat gactcatgaa atgacttggc aactcgcttg
    32821 taggaaggca ttcgttttcc agcccccaca aaagttccag tgttttgcca tcttcagcaa
    32881 ctcgaacgat tccaagattc tcggcaggtt cgctcgctac atttttaaaa tatcttcctg
    32941 aaccagttac aataaaatag catcctgcta aacggctggc atcaaaaatc atcgagatgc
    33001 ggcggatcac tttttcagga tccaagaacg gtgtgatctc ttcttctttt agtaaataag
    33061 aaatattccc gccatttctt tcatcccatc ccaagcgata aagattcgac gtcgtttcga
    33121 tcatttcttt cacataaggc gcttgtaaaa tatctatttt tttcaatata gccacactcc
    33181 tattttctta attccaacac gtctttttga tattcctgga cttcttccag ccaatccaaa
    33241 ccaactggaa cttgattttt gtaacagaaa taattccata catcggcata cgggaaatct
    33301 ttcaattctt ccgtataagc cattcttgaa gtaaagtcaa aagccagctc catttctttc
    33361 aatcgctcaa ctggttccag catagctttc agcagtgctt tttgtgtatt tctcgtaccg
    33421 atgacccaag cagcgatacg gttgatcgtt gcatcaaaga aatccagacc gatatttgtt
    33481 ttttccaaca attcgttacg gactaattct ttagcgattt cctgcaattc atcatccatc
    33541 aaaaccacat ggtctgaatc ccaacgcact ggacgggaaa catgcaacat gattcctttg
    33601 ctgaataaag ctaatgaaga taatttattg gaaatcactt ctgttggatg aaaatgtcca
    33661 gcatctagac aaacaagttt gtcacgagtc aacccgtaac ccatataaaa ttcatgggaa
    33721 ccgactgtat aagcttcagc gcctaaacca aatagtttgc tttctactgc atcttgtaca
    33781 taccgttcat ctaactcttc tgtaaaaatt tcatcgagtg attccattaa acgttcccgt
    33841 ggggtaaaac gatcaatcgg attatctttc atcccatcag gtacccaaaa gttattgata
    33901 cttgtttgcc cagtttcttt cccaaagtac tccgcgattt tgcggcttct tttcccgtgt
    33961 tcgatccagt attcacgaac ttctttatct ggatgagcca atgtaaagcc gtctttcatc
    34021 attggatgcg aaaagaaagt cggattgaag tcaagaccga tttcttcttt ttttgcccac
    34081 tctacccatt tttcaaaatg tttcggctca atttcattta gatcgacaga ttcttctgta
    34141 tccaaataga tagcatgaag gttcaatttg tgtttgcccg gtatcaacga ataagccttc
    34201 tccagatctt gtctaagctc ttcaggtgtt cgtgcggctc ctggaaaatt ccctgtcgcc
    34261 atgattccac cggtcaattc gccatccggt gtcaagaaac ctttgacatc atctccttgc
    34321 cagcaatgca tagaaatttt gatgtcctgt atcttttcta gtacagcatc tgtatctaca
    34381 ccgatagttg catatttttc ttttgcttgt tcgtaatttc tttcaatcgt gttcatacga
    34441 ttactccttt gattttattc acgtaccatt ccatcgtgtt ttgcttattt tcatctgggt
    34501 aaaaagtttc acatggaaac gactgtttga ttacatttct tgcttgttct atattcgaaa
    34561 actgacctgt actgagcatt tgcatggcaa cattcccgat tgctgtggct tcactaggtc
    34621 ctgcttctac aatgattccg gcataatctg ctgtcaattg attcagtaga cgattattcg
    34681 atccgccccc tacgatgtgc agcttttcca atttcgttcc agttaagtgt tctactttga
    34741 tcagctcttg tgcgtaacaa agagcaagat tatcatacac acaacgagcg atagctcccg
    34801 atgagacagg gactggctgc tctgtttcaa aacagtattg ctgaatctct tctatcatat
    34861 ttgggggatt caaaaatcga gggtcattga tttccatcac ttgctgcagc ggtttttctt
    34921 ttgcagcaag ctctgccaat tccgcataac tgtactcgta ctcttgcatt cgggcaactt
    34981 cctggatcaa ccacatcccc atgatgtttt tcaaaaaacg caccgtgtta tgggcacccc
    35041 attcattcgt ataattttca gaaaaagcat tgcgagaaac attggcgatc tttgtttcaa
    35101 tccctaaaag cgaccatgtc ccactgctga tataacccca acgttctcct tgacctggcg
    35161 ttcctaatac agctgacgcc gtatcatgac ttgctactgt gataaaggtc acatctggca
    35221 aatcatattt tgaaaattgt tctttttgca gatctcctag tatcgttccc ggctctgtca
    35281 atggtggaaa taattccttc gagacaccga ttttttcaag aagttctatt tcccattctt
    35341 ttgtccctgc attcagcagc tgcatagtag atgcattagt tttctccgtc accatgttac
    35401 cagtaaagac ataccctaaa taatcaggta tcagtaacaa tttatgtgct tttgccaaat
    35461 tttctttttc ttcagcaaac aattggaaca aggtattgaa aggctggatc tgaatccctg
    35521 tctgctcata caacttatct agagaataga tttcagaaaa ctgttctact gctccattgg
    35581 tacgcttgtc ccgataagca atcggatcat ttatccgatc cccttgttca tcaatcagac
    35641 agtaatcgac tgcccatgta tcgatccccg caaaaaggcg atcaatccct gtctgcttta
    35701 atttttcaaa tccaatcaaa atctctttga taagataatc gatcttccag cgatcgtgac
    35761 cgtctttgtt ccgaaagccg tttttgaacc ggtgtacttc ttccaagatc aaagaacctt
    35821 ttgcatccct cgtacttttc attagtcgtc cgcttgatgc accgatatcg atggcaacaa
    35881 atgttggatt catttctcca cctcttttac tttattgttg attagcactt tattaataaa
    35941 gcgttatcat ttattgacaa tgctatcata atacagaatc ttatattttg cttcacacta
    36001 ttttggtata taattgcatt atcttgacaa gttgggagtg tttacatgga ttactgggaa
    36061 aaagccaacc actcatggtc ggaagactca aaaagagtga tcttgacgcc tacacaaaga
    36121 agcaggaatt tattttttta tatccaagaa atcggttact tcaaagcacc aatcccttac
    36181 tatactgaac gagcacattt accttcttac ttgataaagt ttactttgtc tggcaaaggt
    36241 tcgcttacat ataatgataa tcagcaacag ctaaaagctg gagacttatt ttttattgac
    36301 tgccaagaat atcagcgtta tcaaacgatc agcgaagaac catgggaaat ggattggatc
    36361 catttgaatg gtcagaacgt ccaatcattt tatcaggagt tcacaaaaga cggccataac
    36421 accttccatt caacaggaga tccatcagaa aacaagatcc accatttgat cacagaattg
    36481 atccacatgc aacaaggcac gaatgcccgt accgatttcc agtcctctgt agctatccac
    36541 gagttgctga atgaactgat tttgcaaaag tatcagctag atttcgaaga agaggatatt
    36601 cccgcctacg tttacaaact gaagcaatac ttagacgaac atttcaaaga aacgattaca
    36661 cttgatcaat tagaacaaca attcttattg aataaatatc agctgagcaa agatttttcc
    36721 aaatttatcg gttctccacc aatcgattat ttgatcagca aaaaactttc ttacgctaaa
    36781 gaccttcttc ggtatacaga atttacggtt cgaatgattt ccgaagaaat agggattgag
    36841 aattttgctt actttagccg cctgttcaaa gcaaaaacag gactatcgcc gagagacttt
    36901 cgtaaattca gctgattcat ataatatgta cgtcattttt tcaaaatatt ttgaaatata
    36961 tagataaaaa taaaataacc gcagtttctg cttctatgta aacaacagca gctactgcgg
    37021 ttatttttta gtgatcattc tcattgttca tcttcaaaat gttcataatg aaccaactgt
    37081 ttctgtgtat cacgtttcaa aaattcttta ggcgttatgc cgaactctct cttgaatgct
    37141 ttcagaaaat gcggataaga agaaaaacca cttttactat atacagcact agagctgcgg
    37201 tattctcgca acaagtgctt ggcatagatc agctttttct tcattacata ctgatgaaac
    37261 gtaaatccag tgtacttttt aaattctcgt gtgatatggt atttgctgac aaaaaactgc
    37321 ttttccattt cctctaaaga aagcggatga gataaatttt ttgagatata ttgtaacatt
    37381 tgttccacac gctcattagg aactacagca ttctgttctt gcgcactctc ttctttttgt
    37441 acaacttgat tgagaaaaat caagtattct accagagctt gttcatattt tatgtcttca
    37501 ccgtaaattt ttttatcagg aggatggtcg ataaatgata atttttgctt caatacttcc
    37561 tgttctattt gcaatacacg actcctcttc tctcccattg gttgaaaaca agccgataga
    37621 ttagaccatg gcgtggaacg actttctaga aaagaagggg tgacaaagat atagacacgc
    37681 tcgaatacat ctgaagattg gcggacgata cgatgcaaat cattggaatg gatgagcagc
    37741 acacttcctg ctttgatcgt aaattgtcgt ccgttcaaat aaaaagtcgc ttctcccttt
    37801 aatgtagcat ggatctcata gaaatcatga tgatgataga tcgttttatt ttggctgacg
    37861 acatctttga catgatagat ttcatactga tcagaaacca tcgtattgac ttgttcaccg
    37921 ctgattttct tttggagcat gtgccgcacc tccttttgtt cacttatgca caattttatt
    37981 ataccacgtt ttttgtaatc aaaaagagct gcttttatat actcttataa aaaagaagag
    38041 acgtggcatc ttctagcccc gccccctcca ttctaaatat tagttaacca acgtcttctt
    38101 tacattattc aagaagactt cttccataac gaatcccatc atcttttgtt tcttcaagaa
    38161 ccacatacag gtacggacga ttcttcatga tgatttcttt gatttttgtg tactcgatca
    38221 caccgtctcc caaatttacc ggcacgattt tttcctgttc gacacgaaaa tctttcaaat
    38281 gaaccgcaca tatcttttca ccaaaacgtt caaaagcttc ttctaccaat tggacttgtt
    38341 cttgataatc actacttgtg atcaaattgg tcggatctaa aatgattcct agatagtccg
    38401 aatctactgc ttggatcagc tgctctaccc ttgctaaaga atacaccgga tgattcaaac
    38461 ctggttctat ccccaccatc atttggtatt tttctccagc ttttaccaag cgacggataa
    38521 cggacacagc ttcagcaaat gcttcatctg tgaaattttc ttccgtatat tggatttccg
    38581 gcaaaacaca tccagtttcc gaagcaacca tcgacgcacc aaaggaagaa gcatagcgaa
    38641 gatactgctc gaactttgtc agcaactctt gtcgtatcgt taaatctgga tgaatcatat
    38701 tgatatagca acttaaaatt cctacggtca cttcttgctt ttctaaaact cgtttgacat
    38761 aatttcccat cccagaattg atctcatttg cgccagaagg tatttcagga aacgaaagac
    38821 ctaaggcgag ctgcagtgta tgaatccctt gttcttttgt tttttctgca agatcttcta
    38881 tcgtttggac ttcggaaaga tcatgtcctc taattgtaat gttcaacata actattctcc
    38941 taactcagca tattgggtag ccacatgacg atttgcggga agtaagtaat caaaaacaag
    39001 acgactaaga taaccccata aaaaggaaga agcggtttca gcattgcctc tgcttttacg
    39061 cctcctacac ttgcaccgac atatagtcct gtacctactg gcggcgtgat ggagcctaca
    39121 cacagattca tgattgaaaa caacccgtaa tgtacaggat caacccctac acttgtgacg
    39181 attggtaaaa atattggtgt aaaaatcaag atagcaggcg ccacatccat aaacataccc
    39241 accagtaata acacaagatt gactaataac agaataataa atttattatc cgttacgccc
    39301 ataatcaacg aactaattgc ctgaggaata ccggtaaacg ccatggcgaa agacatcatg
    39361 gaagaagtag ccaataagaa catgatcgtc cctgacattg ctactgattg aaacaacata
    39421 ttaggtatat cttttagttt taccgtttta tagtaaaaca tagcaatcaa cagggagtaa
    39481 atgacagcta tcgctgaagc ttctactgct gtaaatatcc ccgtcaagat accaccgata
    39541 atgatgacaa tcaatagaag gctaggaatc gcctctttta ttatttttcc agcatgagcc
    39601 atctgtcctt tttcgacaac agggtattta ttctttttag caacgatgaa tgctactagc
    39661 atgattccta gcgcccacaa agcgcctacg agataacctc ccataaacaa agcagagatc
    39721 gaagcaccac cagcaacaag tgaatacatg ataaaagcag tacttggagg aatgaccata
    39781 cctgtcggag ctgacgcgat attgaccgct gtcgcaaact tccggtcata tccttcttcc
    39841 acttgctgag ggatcaacac tccgccaatc gcggtagaag cggcaatggc cgagctagag
    39901 atggaaccaa ataaagcatt ccctaacaca ttcgtatgag ccaaagcccc cggcacgcgc
    39961 ccgacgaaca acatcgcaaa atccactagt ttcttagcta tccctccatt attcatgata
    40021 atgcccgaga gaacgaaaaa cggaatagcg atcagcgaaa aactattgat acttcccacc
    40081 attttttgag ctgaactaaa agcagaaaca tcaaacggta taactaacaa taaagtcatc
    40141 atcgatgaga atgcaatact tatagcaatc ggcatcccta atacaagcaa cacaacaaat
    40201 acaaaaaata acactaatcc ggcttctatt gccattttat tcctccattt ttatattttt
    40261 tcttgaattg ctggcttgtt gcttaatgta tcgattagat tgatcaggct atagataatg
    40321 atgagtaccc ccgatacagg tactgacaga taaacatatc ccataggaat acttaaagaa
    40381 ggcgagatct gccccattgt caatgtgaca gcattccatc cgccatacac catgatcatc
    40441 gcagcaaata ccagaaataa actttgaaca acaacttcta aaattttact ttttttgcca
    40501 gttaattttt cacttaaaac cgttacggct aaatgtgctt tttgtcccac aacatacgcg
    40561 gaagacaaca tggcaaacca gacaagaccg aatcgaacaa gctcttccgt gaccgtattt
    40621 ggatttccta aaaccgttct ggaaaatact tgccataaaa caacagcgac catcccaaca
    40681 agcaaaacag ctcccaggat ctctaatacc ttgtttaaaa tcattttcat cacagctacg
    40741 ctccttgctc ttggatcttt tgataaaggt ctttgtaggt aggatcgttt ttgaatatat
    40801 catgaagtgg ggcaactgcc tctcgaaacg gtgtattatc tacatcattg aattggactc
    40861 catgttcttc ttgtgctaaa gctttttctt cttcaattgc ttttgcaaaa gcttcaattt
    40921 caaaaaccgt tgattctttc gctgcttctt caatggcttt ttcctgctca tcagttagct
    40981 gttctttgat ggcatcattc atgatgatga tatctggcac acgtgtatgc tcatcatagg
    41041 aatagtattt agccacacca ccatgtccag ctgtatacaa gacaaattca ttgttttctg
    41101 atccgttgat caaattggat tgaagagacg tgtacacttc atcacttccc ataggtacgg
    41161 gcgaaccgcc taaaagctct accattttga tagcggtttc gctttgcatc acacggatct
    41221 tctttccctt caaatcttcc ggcgtatgga tcggaccgtc taccatataa aagctgcgct
    41281 gaccagaatc ataataggtc aatccggtaa atcctaaatt tttggtcgat tggtagattg
    41341 gctccattat ttcttcatta tccatcacct taaagaaatg ttcttcatca ttgaatagat
    41401 aaggaactga aaaaatcgaa taatctttag agaaactttc caacgctgag gcacttactt
    41461 ttgtgaaatc tactgctcct gactgtgtca attcgatcag ttcacgttct ccacccagtt
    41521 gtccatctgg gaaatactgg atctgtacgg aaccatctgt tttttctttt actaaacgcc
    41581 caaattcatt cattgcatca attactggct ggctgttgct tgcataagca aagcgcaagg
    41641 taactatctc atttacatct gcatctgcga cagttgccac actagaatta tttccgcagc
    41701 cgcttagacc tagcagacct aggctaagcg aacttgctgc aaacagcagt gatgtttttt
    41761 tcatatgggt ttcctttctt ttaacaataa gagacgagca attctgtcaa acaaagtacc
    41821 gttaacgact gtccataagg cattgctgtg attccgatat tccgataaaa atctaaactg
    41881 tcccccattc ctgtcccgat tgaaacgttt tgaacttctc ctttttcatc gatttgttca
    41941 agcaatcctt ggatagcacg ataagcgact tctttatatt cttgtggtaa ataacgttta
    42001 tggaccgctt tcaaaatacc gtaagcaaat cctgcagttg cagaagattc tacgtaagaa
    42061 gaaggatcat ccaacaacgt atgccataaa ccgctttcag actgataagt ttttaatgca
    42121 cgcacttgtg cttcaagtgt ttcgatcaat aatttgcgca atgcatcttc tttcgataat
    42181 tctaagattt caatgatctc tggaatagca atcgtcaacc agcagtttcc acgagcccaa
    42241 aatgcttcgg catagttatg atttccttca aacgtccagc cgtgatacca caatcctgtt
    42301 cgtttatcca tcaagtattt gatatggatc ataaactggt aacgagcttc ttctaaatat
    42361 tctgggcgat tcaacagttt cccgattttt gccaaaggaa gcactgtcat catcaatgta
    42421 tcgtcccaca actgattctt attttccggt ccatatgtgg catgctgtaa cccgtcttca
    42481 tttgtccgag gcatgtcatt catgacccat tctgcccatt gttctaaata aggcaaatag
    42541 cgtgaatctt tcgtgtcttc atacaagtaa gccatagtca atagcggtgc cattgtattg
    42601 acgtttttgg gcggagcgcc ttcttccata cgatcctcaa accattcatt gaccacttga
    42661 taagcgcgag gattttttgt catctggtaa tttttataaa taccatatag accgacacct
    42721 tgtggccaat tccacactgt ccagcttttg tcatctactt tcagtccatc aaagtccaac
    42781 aaaaattcac cattatcgtc tttgatcgtt gtcaggtttt caacgagcag gtcaattttt
    42841 tctttcacta attctttagg tacaaaaatc tcttctttaa taatcatctt tacgccacct
    42901 ttttaaacgc tttcatttta tatccttaat atagcagtaa taccgtccac tataaatatc
    42961 gctttttgaa caaaaactgt ctaaaattga cctaaatgtt aaataacaaa agtaactttc
    43021 agttacttgg aaattaaaaa aagaacttgg agaaacaacg ttttccaagt tctttttcat
    43081 ctattttctt atcttttttc tacgctttct tttgcttatc cgttgtaagc atcaatgatg
    43141 atctgtttca attcagagat caatggttct ttcggattag ctgtcgtaca ttggtcttcg
    43201 taagctaatt cagccatacg gtctacggtt gtatctagtg tttcttgtgt gactccttga
    43261 gcttttagac tcatgttgat gccgacagat tttcctaatt cataaacagc cgttgctaaa
    43321 gcttcgacta attcctctgt tgtttcgcct ttcagaccta agaattttgc gatatctgcg
    43381 taatctgtat ctgcacggaa atagtcatat ttagggaaca tcgcatgttt ttgtggatct
    43441 tttgcattat agcggataat atgcggtaaa agaatcgcat ttgtccgtcc atgagggatc
    43501 ccgtattccc cgccgatttt atgtgcgact gagtgacaaa tacctaggaa tgcgttagca
    43561 aacgccatac cagccattgt agaagcgttg tgcatttttt cacgtgattc catatcaggt
    43621 gtttttactg atttttctaa atagtcaaag actaacttga ttgcttgtag gctcaatcca
    43681 cgtgtataat ctgaagccat gactgaaaca taggattcga ttgcatgtgt taacacatcc
    43741 atcccggtat ctgctgtgac agatgctgga actgacatga cgaattgagg atcgacgatt
    43801 gcgacatctg gcgtcaatgc ataatctgct aacggatatt tcacatgtgt ttcactgtca
    43861 gtgatgaccg caaatggcgt tacttcagaa ccagtccctg atgttgtcgg gatacatacg
    43921 aattgtgttt tttctggttt gtcgatctta tatgtccgtt tacggatatc caagaatttt
    43981 tgttttgcac caaagaaaga tgtgtctggg tgttcataga acatccacat tcccttagct
    44041 gcgtccattg cagaaccgcc tccaagagcg attactgtat ctggcttgaa gtctgccatc
    44101 agtttcgttc ctgcgtatac ggtatttgtc gatgggttcg gttcaacatc tgagaagact
    44161 tcgatcttca catcattttt acgtcgtgcc aatacttcac gaactgtatc gcagtaaccg
    44221 aattggacca taccagggtc acaaacgatc atgacacgtt caacgttttc cattttctct
    44281 aagtacaata gagagttctt ttcaaagaag atttttggcg gtaatttgaa ccattgcatg
    44341 ttatttctcc gtttcgctac agttttgaca ttgattaagt ttacagcaga gacgttacga
    44401 gaaacagagt tcttaccata agaaccgcat cccaaagtca atgacggaat catttcgtta
    44461 tagatatcac cgatgccgcc ttctgcagat ggtgtattca ctaaaatacg acaagctttc
    44521 attcgaagac cgaatttgac atgcaactct tcgttttctg aatggataac tgctgtatgt
    44581 cctaatcctc caagattcaa cataccttca cacaaatcaa acgcatggtc cgtgttgtta
    44641 gctttgatca tggcaagaac tggagaaagt ttttctcttg atagcggata atctgggcct
    44701 acgccttcca attcagcaat cagcattttc gttccttttg gtacactgat tcctgccaat
    44761 tttgcgattt ccatcgcaga atgtccaacg attgatggat tgaccgcata ttttccttcg
    44821 ttcatcactg catcttctaa tttttgcaat tcatctggtt ttacgatata aacttggtgt
    44881 gcttggaatt ctgcttttac tgctgcatag atttctttat ccacgatcac agcttgttca
    44941 gaagcacaaa tcattccatt atcaaatgtt ttcgaaacga tcaaatcgtt cactgcacgt
    45001 ttaatttttg ctgttttttc aatataagct ggtacgttac ctggacctac acctaaagct
    45061 ggttttcctg tagagtaagc agacttgacc attccagctc cacctgtcgc taagacgatc
    45121 gcgatacctg gatggttcat tagcatagaa gtcgcttcga tcgaaggatg ttcgatccat
    45181 tggatacaat tttcaggagc tccggcagcg atagcagcat cacgtaccac tcgtgctgct
    45241 tctgcagaag atttttgggc acttggatgg aaagcaaaaa cgattggatt tcttgttttc
    45301 aacgcgatca ttgatttgaa aatcgttgtg gaagtcggat tcgttgttgg agttactccg
    45361 caaaccacac cgactggttc agcaatttca atcaaccctt tttggacatc ttcattgatc
    45421 acaccaactg ttttatcatg tttgatatta tgccaaatat attcggacgc atacatattt
    45481 ttgattgctt tatcttcgta aacaccgcgg cctgtttcct ctacagccat cttcgctaat
    45541 ggcatatgtt gatccaaagc tgccattgcc atttcatgca cgatatggtc tactttttct
    45601 tgatcaaagc tttccatttc tttcaatgct gcatttgctt tttctgccaa atcatcaatc
    45661 attgcttgta cgtcaattgt cttcgttttt tctttttcta ttactttagc catcattatc
    45721 ctcctaatgg gtcatggtta tttgttaaat aattcacatg tatatcatac aatatttatt
    45781 tttctatgta aacacttttt tgtgattttt ttcacagtta tttttaaata taaaattgct
    45841 ttatttacag gtgtttgtat agaaaaatat ataaagttat ttattattat ttatatgaca
    45901 cacccgatta ttcaatattg agcaatatat cccatgctct tatctctttc ttttttgaat
    45961 aaaaaatgag gtaggacatc tttctgccct acctcgtctt taaataagct ttgatactta
    46021 tttttcttcc ttctcttctg tctcttccag cgtttctgtt gtttcagttt ttacttcaac
    46081 tgtctcagct ggttcttctg ttgtttcaac agtagtcgtt gcatcatttg ttacgacagt
    46141 ccctggtttg accgttttga ttgctgcacg atcgtactca aggaagatcc cttcgcagtc
    46201 taaaacaact gtccgtttgt cattgtcgat ttctgaaaca actccatgta atccgccgat
    46261 cgttacgact gaatcacctg gtttcatgct atctaacaag ttttgacgtt cttgctgttg
    46321 tttcttttgc gaacgtgaca taaaaaacca cattgctaat agcgcgataa acatcacaag
    46381 cattggtaat gttcccattt ttttcacctc actaattctg tatctacaaa aaacacttta
    46441 gcagaaaaat gctgataaca ctagtttttt acttaatttt taaaagattt tcctttagaa
    46501 gctttttgcg ttttctttat tgaacccgta ttcttcaaaa aaggcttctc taaattcaag
    46561 caaattatca tccatgattg cttgacgtac ttgtttcatt agattcaaca agaagtataa
    46621 attgtggtaa gaagtcaaac gaatcccaaa tgtttcatca catttgatca aatggcggat
    46681 ataggcacgt gtatagtttt tacatgtata acaatcacat ttctcatcaa ttggacggaa
    46741 atcatgggca tatttcgcat ttttcactac tagacggcct tgagaagtca tacatgtgcc
    46801 atttcgagcg attcgagtag gcaagacaca gtcaaacatg tctactccac ggataacacc
    46861 atcgatcaat gaatcagcag caccgacacc catcaagtaa cgtggtttgt tttcggggat
    46921 caacggtgta gtgaattcta atacacgatt catttctgat ttaggttccc cgacagataa
    46981 cccgccgatt gagtaaccag ggaaatccat cccgactaga tctttcgcgc tttgacgacg
    47041 aagatcttca aaccctgctc cttggatgat tccaaataat ccttgacggt ctggattagc
    47101 atgagctttc aatcctcgtt ctgcccatcg agacgtacgt tctactgatt ttttcacgta
    47161 gtcgtaactt tcatcgaatg gtggacattc gtcaaaactc atcatgatat cagagcccaa
    47221 tttattttgg atgttgattg ctttttctgg tgataaaaac atacgcgaac cgttcaagtg
    47281 atttttgaaa tgcacgcctt cttcttcgat attccgcata tcacttaatg aaaaaacttg
    47341 aaagccgcct gaatctgtta aaatcggttg atcccagttc ataaatttat gcagtccgcc
    47401 agcttcttct actaagtctt caccagggcg tagccataaa tgatatgtat tacttaaaat
    47461 aacgccagca cccatttctt tcaactcttc tggggacatt gtttttactg tagctaatgt
    47521 tcctactggc atgaacattg gtgtggggaa agttccgtgt ggcgtgatca attcccctaa
    47581 acgcgcgcca gtatgttttt ctttcttgat taaacgatag cgaatggcag gttgcgtcat
    47641 tcatttccct actttctata taaaatatct tgacaactcc caccatcata atcgtttcaa
    47701 gtgctttttg caagttccct cggcttcctt ctactttatt taatattctt ttttttatcc
    47761 tttttttatg aataacaaaa aaaagaaaaa cattatgaca gaaatatttt ttgatacatg
    47821 gtatattggt gttattaaag aaaaggaggg aaaaatcatg caatcttctg cagaattaaa
    47881 acgccaagca aaagagagtc taaaaggacg ttggggacaa gctgttttac taaatctaat
    47941 ccctacattg attggtattg ctactattct tcttattatg attcctatta tcttgtttgc
    48001 caccaccata tatgatggat cagcgagcgt ctcagaagcg acaacttcag gaggcggtag
    48061 ttcaggaggc ggtggcggaa ttatttccag catcattagc gctttgttca tgagcggtat
    48121 ttcttggact tatcttgata ttttacgtgg acaaaaagat cagatcgaac cattcaaaga
    48181 tgcatttcgt ggattcaaag gtttgtttat cggcggagtg atcagcttag ctgtgctgat
    48241 ctcgatcttt acaacattat ggggtttgct attagttatt cctggaatca ttaaaagcta
    48301 ttcttattct caaagctatt ttatttacta cgatatcgtc caagaaacag gacaaacacc
    48361 taaagtactg gatacgatca ctgcaagtcg aaaattgatg gatggttata aaggacaact
    48421 tttctggtta gatctttcat ttatcggctg gcatatctta gcattagcaa ctgctggtat
    48481 tggttatcta tggctcagcc catacatttc tgcaacaaaa gcagcatttt atgaacaatt
    48541 gccaaaagat atttaaaaga gagtgtgact tgtgaccgag ggggtggcag gagaacaccg
    48601 tttatcgaga ggcagtaaaa gaaacattca gcccattagc aaaaaggagg tgtgacaaca
    48661 ttcatgtcac acctcctttt tgtatagttc cagtaaaaag aaaatcaact tagaaacagt
    48721 taatcattcc tcttcttctg cttccggtaa tcgatggaac ttgatcagtt cattcaccgc
    48781 gattggtacg atagataaaa tccctaccaa gaaccagtga ttcaaactga tgtgtactag
    48841 gccgaatagt tcttgcgtaa atggcagcaa agcaacagcc gttgtaatag ctaatgccag
    48901 taacgccatt tctaccaaag ctttattggt cgctaaattg actttgaaga tcgattggct
    48961 gctgcgtaca ttgaacacat gcagaatcga tgaataagct aagatcaaga atgcaacagt
    49021 ttgaccgaca tgctgacttg cgtctaccca tgggctgata gtatcgacat aactacctaa
    49081 ataataacca aacaaggtta cgatggtgaa gacccctgca tttatggcaa ttttttgcaa
    49141 taaacctcga gcaaagatcc cttcattttt cggaatgggt ggctgatcca tgatgccgtg
    49201 ttccgctggc tctcttccta aagcaaatcc tggaagtcca tcagcaacta cattgacaaa
    49261 cagtagttgc acagctgtaa atggtgctcc ccatcctaac agcatcgcaa tcaaaacaat
    49321 aaagatctcc gaaatattac agcttagcaa aaagttgata gctttacgaa tattctgata
    49381 aaccgaacgt ccttgactga cagcgtctac gatcgtcgca aagttgtcat ccgtcaaaat
    49441 catatctgcg gcactttgcg ccacatctgt tcctgtgatc cccatggcac acccaacgtt
    49501 acttgctttc aacgctggtg catcattcac accatcccca gtcatcgcta cgactgcacc
    49561 cgtacgttgc caagatttga cgatccggat cttgtcttct ggcgttaccc gagcatagac
    49621 agagagagct ttgacacgcg catctaattc ttcgtctgat aattgatgca gttctgatcc
    49681 tgaaagtgct tctgacggat cattcaagat acctaattct ttcgctatgg cactggcagt
    49741 caccacatga tctccagtga tcatgactgt tttgatgcca gcctttttcg ctcgcttgat
    49801 tgctccttta ctttctggac gtggtggatc gatcattccg atcagaccaa tcaaacgtaa
    49861 gtctttttcc agtgcttcag aagaaatatc ttgcggttct tcgtcataag tagcataacc
    49921 aacagcaatc actctcagtg cccgtttacc aaagcgatca ttaacgatcg ccgcttgttc
    49981 tacatcgcct gaactaaagc gcggagcaag aacatcgaat gctcctttgg tgattgagat
    50041 gtatttttcg cccatttggt gaactgttgt catcattttt cgttcagaat caaaaggaag
    50101 ctcgtttact cgtggatatt tttcttctaa ttctgccttt gtatgataat tctcttcaac
    50161 agcccttacg atggctactt ctgttggatt acctttgata acaaggtcgt catcttcttg
    50221 ctcgaccgtc acatctgtac atagtgcagc cattttcaat atttccatcg cgctgttcgt
    50281 cattgaatct tcgatattcg ttacttcatc ttctttgctc catacccggc ggacgcgcat
    50341 ttcattttgt gtcagcgttc ctgttttgtc tgagcagata acattagcgg ttcctagcgt
    50401 ttcaactgct ggcaactgtc ggatgatcgc atgtttcctc gccatcttct gcacaccgta
    50461 tgcaagtgtc aacgtaacaa taacagtcaa ggtttccggt acagctgcaa cagccaagga
    50521 aatcgaagtc atgaacatct caaggagtgg ttctccttgc agttcaccaa tcacaaatac
    50581 taaagccgca gccgctaaag cgatcatact gattcgtttg cctagctgat tcaatcggac
    50641 ctgtaaaggt gttttttgca tagtattttc gttcaaaaga tccgcgatct tacccatttc
    50701 tgtagccatg ccaatggcag tgacgatcgc tttcccacga cctgcagcaa ccgtacatcc
    50761 tttaaatacc atattaagtt ggtcacctaa tgactcatct tcttcagcaa taaatgctgc
    50821 atctttttca actgcttcac tttctccagt caacgccgat tcttctacac gtaattgtga
    50881 tgcttcgatc aaacgggcat cggcagaaat agcatcaccg gactctaaaa caagaatatc
    50941 acccactacc agttgctctg actcaacagt ggtcactttg ccgtctcgta tgacagttgc
    51001 cgtttgttta ttcatatctt gcaaggattc cagtgctttt tctgcgtttc cttcttggac
    51061 aatcgccaat accgagttga tcacaacgat agcgatgatc agcagtcctt caaataaatc
    51121 cccatgttct gtagcaaatg cggtataaaa ggaaatcacc gctgctacaa gaaggatgat
    51181 cgttgtaaaa tcggacaagc ttcgaaagaa ttttttgatc atcgattctt tcggcgcctc
    51241 atcgaattta ttcaatccct gcttttttcg ccgttcatcc acttcttgtg aagacaaccc
    51301 agtatctgga tctgcttctg ttaccttctt tacttcttct attgtctgct tgtattcttc
    51361 catcctattc ctcctttaaa atcttcgtac aaaaaaaggt acttatttta ctataacatc
    51421 ctttttttat ttacttcaaa taaagggctt atcgattatt tgttaagaaa gcgtaaaatt
    51481 taaagtgctt atatttgcaa acgctaccaa aggatgctat tatatagatg taaggaagga
    51541 ggataccatc aaatagacag aagaatttct cgatagaaca gtgtaagcaa acaaagttgt
    51601 tagaagtaca ggtttcaccc agacatgcgt gtagagtaca aacctataaa tagtgaatgc
    51661 gtatggatgt ttggatcacg aagtatccga aacaagaagt aatgcgatac gtaacaattg
    51721 aataacgtaa acgtctgaaa attgaatagg aacaagagat gtttcaaatg ttgaagatgt
    51781 aacgaaaaca aaatttttgt ttaacatgaa gaaattcaac accgtgcttt gcatgtatga
    51841 tggaaagtta caatcatttc tgtttatttg aatccttccg aggtacaaga aagccgagct
    51901 tatcgagagc tcggcttttt ctttgtcata aaatctttgt aaagcttttt tgttattcaa
    51961 aactagcggc ttttcataac cttatttcac gaacatcgca tctccaaaac taaagaaacg
    52021 atagcgctct tcaatcgcat gctggtaagc agataaagtc aattctcttc ctgcaaaggc
    52081 actaactaac atgactaaag tcgattttgg caaatggaaa ttagttgaga aagcttctac
    52141 cactttgaat tcatagccag gcgtaatgaa aatatcagtc catccgctgt ctgctttgat
    52201 ttctccatta aatttcgttc caatcgtttc taatgttcga attgaagttg ttccgacagc
    52261 cacgattttc ccgccatttt tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa
    52321 acggtagaat tcactatgca tatggtgttc ttcaatttta tctacgctga caggtcggaa
    52381 tgtacctaac cctacatgta gtgtcagata gactaattca acaccctttg cttcgatctc
    52441 atcaagtaat tctttcgtaa aatgcaatcc agctgttggc gctgcagcag aaccattttc
    52501 tttcgcatag acagtttgat agcgatctgg gtcttccaga cgttctttga tatatggagg
    52561 caatggcatt tctcccaatg attcaagaat ctctagaaaa attccttcat actcaaagtc
    52621 aatgatccgt ccgccgtgtt ccaattcttc tttcactact gcttgcaatc tgccatcacc
    52681 aaaagagatt cttgtcccaa ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt
    52741 atctccttgt gtattcgtta gaagaaggac ttctaaatgc gcaccagtat ctggtttttc
    52801 tccatataaa cgagcaggca gaactcgtgt attattcata actagcgcat caccaggctg
    52861 taactcttca atgatttcat ggaaatgttt atcttccatc tctccagttt tccgatccaa
    52921 aacaagtaaa cgagaatgat cgcggtcttt taatggtgtt tgtgcgatta attcttccgg
    52981 caagtcaaaa tcaaaatctt ctgttgttaa catatcgtca ctcactttct atcatgtgct
    53041 tcatcattct atcatgtttt tcgttctgtt ggcaaaatca gcactttttc acttctattt
    53101 tttgttttcc taaataaaaa aactacccta tttgtatttt ttcattgttt taggcatact
    53161 agtaatagat agttcttcat tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa
    53221 aatgtgtcaa aaatttataa aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt
    53281 gaaaagggtg agttcatttg ttttatcggt acgagcggaa gcggaaaaac gacgacgatg
    53341 cggatgatca accgaatgac tgatccaaca caagggaaaa ttttgattga tggaaaatcc
    53401 atccaagaaa ttgatccagt ggaattgcga cgtcagatcg gctatgttat ccaaagtatt
    53461 ggattgatgc ctcacatgac gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa
    53521 gtcagtcaag aagaacggaa taaaacagct gaaaaaatga ttgatcttgt ggaattgccc
    53581 cgtgagatgt tagatcgcta cccacatgaa ttatctggtg gtcagcagca gcgtattgga
    53641 gtcgttcgtg cccttgcagc gaatcaagat atcatcttga tggacgagcc attcggtgcg
    53701 cttgatccaa tcactcgtga ttccttgcaa gaccttgtca aagatttaca agagcgtttg
    53761 ggaaaaacga tcgtatttgt aacccatgac atggatgaag cgattaaact agcaagccgt
    53821 atcgccatca tgagtgaagg ccgtgtgatc caatttgata caccagaaaa tattttacgt
    53881 catcctgcaa acgatttcgt agaagagttg attggagaag atcgcctact acaagccaag
    53941 ccaaatgcga caagagtcgg cgaagttatg ctaaatacag caatcacgat tacaccggaa
    54001 aaatcattac aagaagcaat caaactgatg agggaaaaac gtgtagatac gttgttagtt
    54061 accgataatt cgaatgtatt aaaaggattt atcgatgtag aaacgttgaa taaaaaacga
    54121 ggaaaagtat ccagtgtcgg cgacattttg aataaacatg ttttttacgt aaaagaatcc
    54181 gcctatcttc gtgatacctt gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc
    54241 gttgatgaac agaacaaagt cgtaggtatc ctgactcgtg catcattagt ggatatcgtc
    54301 tacgatgtga tttggggaga agatcccgca actactgatg ttgcagagtc agctactcct
    54361 gaagaatcta aggaggtctg acctatgcag cagttttttc atcagcacgg ttcagaaatt
    54421 ctctcaaaag gttttgaaca tctttatatt tcaggacttg ctttgatcat tggtatcatc
    54481 attgctgtac cgcttggtgt cttactgaca cgttttccaa aagctgccac gattgtgatt
    54541 ggcttaacca gtgccttgca gacaatccct tctcttgctt tattggcatt gatgatcccg
    54601 ctatttggcg taggtaaatt acctgcgatt atcgcactct ttatttattc attattgccg
    54661 attttacgta atacgtacat tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca
    54721 aaaggcatgg ggatgactga gttccaatcc attttttccg ttgaattgcc cattgctatg
    54781 ccaacgatca tggctggtat tcgtttagct gccgtttacg tgattgcatg ggctacactt
    54841 gcctcttata tcggcgctgg tggtttaggc gatttgattt ttagtggact gaataattac
    54901 caaccagatc tcattttggc aggtacgatc cctgtaacaa ttttagcact attggctgat
    54961 tggcttctag gtctcttgga acaccggctg acaccgatcg cacttagaga ggagaatgaa
    55021 gaatgaaacg tatactaaaa atgaccatgg ttcttttctc tcttcttata cttgcgagtt
    55081 gttccttccc tggtcttgcc agcaatacag atgaagacac catttcgatc acaggtggaa
    55141 tcacttcaga agcacagatc ttagcaagtc tagttgccgg aatggttgaa cattatacag
    55201 ataaaaatac cgcaatcatc aacaatctgg ctactacaac tatcaaccat caagcgatga
    55261 tgaacggaga tgcttcaatt tcagcggctc gctacacagg aacagattta acgactacat
    55321 tgaaccttcc tcctgaaaaa gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg
    55381 agaaaagata cggtcaaacg tggttcccgt cctatagttt tgaaaatacg tatgtctttc
    55441 ttgttcgaaa agacaccgca caaaaatacc atctatctaa agtcagcgat ctgaaaaatg
    55501 tagcggatga attagtagct ggtgtagata cttcttggat caatcgtaaa ggagacggtt
    55561 atgacggctt ccaagaaact tatggcttct ccttcaattc gatattacct atgcagattg
    55621 gacttgttta tgacgcagtc gaagcaggaa aaatggatat tgttcttggc tactctacgg
    55681 atggacggat cgccagttat gatctagtca tgttggaaga tgatcgtcaa ttcttcccac
    55741 catatgatgc tgctccagtt gtagacaaca agatattagc tgatacgcct catttaaaag
    55801 aagccttaaa taagttaggt cataccatca gtacggaaaa aatgcaggaa ttgaactacg
    55861 aagcagataa taatctagtg gaaccatcag tagtagcaca acgtttctta caagaaaacc
    55921 actattttga agggaagtga agcaattgga taaaatgaat gtctttcaac aatttttcta
    55981 ttatttccag gaaaatggct cctatatctt tgcacaattt atccgtcact tcctgatttc
    56041 tatctatggt gtcctctttg cagcagttgt cgggattccg gttgggatca tgatttctag
    56101 gagacgtaaa ttagcaaact gggtgattcg tttggcgaat atcatccaga cgatcccttc
    56161 tctagccatg atctccattt taatcattgg attaggcctg ggagtcaatg tcgtgattgt
    56221 tactgtattt ttgtattccc tcttaccgat catcaagaat acgtataccg ggatgatcca
    56281 agtagacaaa aacatccttg atgtcggcaa aggcatgggg atgactgcta gacaacgact
    56341 attcatggta gagttaccac tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt
    56401 agttgttgcc atcgggataa ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga
    56461 tatcattatc cgaggaacca atgcaacaga gggcacatcg attattttag caggtgcatt
    56521 gccaactgca ttaatggcga tcatcacgga ctggctttta ggtattttgg aaagacggct
    56581 agatccagcc agccgtacat cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc
    56641 atttgttggt cgcaaaggct tttttagttc tgtcattttt aaggcaataa tccactctga
    56701 ttcttccctc cttatgggca aagagttgct tttcttagct atttatccat tccgtatact
    56761 ccaacttcat actgccaacg ggttcatatc tgcatccacc gactaagttt ttttcttatc
    56821 cgctaaggca ttttgccaat ccattttccc atcactcgtc atggaattca taccacatga
    56881 tctctaaata ctcgtctgct tgttctttga attgggcatc aattcttaca tcctttggcg
    56941 tggctttcat ttaagccttt tccatcagtc ctcgattgtt cgatgccttc agctgcatca
    57001 tcaaaactgg tgactgccgt atcggtaact tcgatcatct tttatacaat cgggaacttg
    57061 ccatcctcat ccttggctaa aaacatatct agccattcca gcttctcgcc atgggaggtt
    57121 tgcggttcct aaaaccggta cacgattctt atggactgca tccgtcgcaa ctagacttgg
    57181 cgatacgatg atccttcacc agaagagcac ccccgtaaac caattgatcg atatattgcc
    57241 aataagaaaa gacattggcg taaaagatat cgatccctcg tgatgcattg ccgttgatac
    57301 tgctattcat ggccgaaagc gccacgactt taacttcttc cgtttgagtg acattcatgt
    57361 tcggcaatgt cgctttggat acccgctctg ctaaaggaac gacactggta ttgtatttcg
    57421 tatctgtatc ttttccaaaa gaccatgcca acgaatcctc tgggaaccaa taagacgact
    57481 ccggttgatt gtccattgct tttttcaaca tacttttggc tgtttctcga taaacgacat
    57541 ttccaattgt cttcgcctca ctttggcgat aatcgatggt cgttgccact gccaacacta
    57601 acaacgatgc tccaatttta ctgattttcc ttgtcacact tcttcctcct tgttttacat
    57661 aaacccgtca acattccatt cctaaactca aacaatgacc cttcaaaggt ggagatatag
    57721 gaacaaatta aagaaatcat gcaataaatc caagaaggtt ggttttagaa caaaaaaaga
    57781 gtctcctttt tggggagact ctttgttaac tatctaaaaa ggttggctct tctcacttca
    57841 ctcataatgt gtccaggcag aataaatttc tacaacttta ttttcctcat caactttgta
    57901 aaccactcga tgctgtctat tcagcctgcg tgagtatctg ccttcatgtg taggtctcag
    57961 cttttcaaaa gactgcgtgg gcatatatgg atcttccttc aatgtctgaa tcacttcttc
    58021 aaattgtttc tttaaattcg tttgcttgat ttttcttaaa tccactttcg cagagttttt
    58081 gattgcgact gtatagttac tcatcaaaga gcttcccaat caatatcatc gatattcgta
    58141 aagctactgt cgtctttttc gcggtctcgt actttatcta aagtacctgt ttgctcaaga
    58201 aaaagggttt cttgaatcgc tctccagtct tccagcccaa tcaatacagc attgtttcca
    58261 ctacgatcac taataatctc gatttcagta tgattttcat ttacctcttt caataactga
    58321 taaaaatttt tacgagcagt gctaggattc atcgttgtat tagccattgt ctctcctcct
    58381 ttttctgata taattatagc gtacttgatt aagtacgtca aaaaaataca ataaagcact
    58441 taaattcagc aaacttttcc tcttttttac atagagaaaa agaccccttt ttagggagga
    58501 tctttgtcca cgctcacttc ttactccttt acgactagcg gtagccaaac gtaaagtaat
    58561 tggtaacaac gtatcatctt tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca
    58621 acaccaatac acgaaggttc ctcgttgcag tgtgacttga ttgatatctt gaataacctg
    58681 aggatacgca cgaaagattt gtaacggtta ttcaaaactg attttggccg tcaagtcttc
    58741 tactgcctca aaaatgattg cctctttcgt ctcctgtcat tcatgttcac tatcgatttg
    58801 atacttgctt agaccgttgg gtttgtgaat caccaattaa aaatggcact gagttatggt
    58861 aacaaaaaat ggtcacttga cgaggctgag attcctactt atgaagacac atggcactta
    58921 agagtcgagg taaaggaaga agctgaaaat ataggaggct gtacattatt tggcaaagga
    58981 ttcggaaatt atgatcaaga catcaaaatc aaaatgtttt acagctaaat aggaagaata
    59041 ataacatttc atacgctgtt cccatcttta atggcttata gagggcacct ttagctgaga
    59101 ataagacaaa acagaccctc tagcttcaat taagagctag agggtctgtt tttgatttta
    59161 aaacttggtt tatttcttca aatggtagct ataggtgctg acaacgatat tttcacgcca
    59221 agacaatcgc aatcttaacc gcaaacttgt ttggttatgg agaatattct gccagcgtct
    59281 gtttggtaca aattgaggaa tcaacacagt agttgtatag ttttgtttcg ctgcattttt
    59341 acttacgata tctacataac ggataattgg attttcaatc gagcgataag aagagtggac
    59401 gattgaaaaa cggacatctg ggaaatgctt tttaaattct gcttcgattt ctttttcttt
    59461 ttcgacatct tcgtctagcg aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc
    59521 gtaattcaac gcaccgatat ttacccgtgt cacattcccg actaatacaa gaacggtatt
    59581 cccgtcataa tcatgtagtt ttgcatcatt ttctaaacga agctgttctg ctactttttt
    59641 ataatgatca tggatcttat aaaaaatgaa cataacgatt ggcataatga tgaagaaagg
    59701 ccaaatgtcc cctaaccgat agacaaacaa aatcgcaatg attgcatatg agatgaacgc
    59761 acccgtaata ttggcaatcg atttgcttaa ccatcttttt ccttcttttt tccatttgac
    59821 gaccatccct gtttgagata aagcaaatgg aatgaacacg ccgatagagt ataacggaat
    59881 caagcgttct gttgagccat ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt
    59941 gataatccca ttggaatagc ctagtcgatc cccacgatct tgatacatgt gaggcatgaa
    60001 tttatctttt gccagattgt atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc
    60061 aactgctaaa atcagcgctg tagcaaattg aaggacgtaa tacaaaatac catgtccaaa
    60121 gacagcttta ccaatttgag acaaaacagt gacttctttt tcaggaacga tcccatacca
    60181 atagttgata aatgtgatcc ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa
    60241 tgtagcagcg gcattttttg ctcttggctt cttaaagaat ggtacagcat tactaatcgc
    60301 ctccacccca gttaaagagg aagaaccaga agaaaatgct cgtaaaatca aagcgataga
    60361 aacacccggc acagccgctc ctggaagtgc agtagcttgc aatggcacag cacctgtcac
    60421 gatattatat aaaccaacaa cgatcagtaa cgagatcaca aggataaatg tgtacaccgg
    60481 aaataatagg aacgatgcag attccctcag cccacgaaga ttcagcatca tcagtagcaa
    60541 aacgattgtc acagaaatag cgacttgatg accgtagagt gctggcaccg ctgatgtgat
    60601 cgcttcagca cctgctgaga cagatactgc tactgtcaac atgtagtcga tcaataaaga
    60661 gccaccggaa atcaagccag cattctttcc aagattctca ctgctgacga catatgctcc
    60721 cccgccatga ggataagcat ggatgatttg acgatatgat agcgttaatg aaatcaataa
    60781 aataataaca aaagcggcaa tgggtagcga ataccagatg gctgcagcag acaaagcgac
    60841 taaaacgaca acgatttgtt ccgtaccata tgcaatagat gataacgcat cagacgacaa
    60901 taatgccaaa gcagcaaatc gggttaattt gtgctcatca ttttcagcag attttaaagg
    60961 cttacctact aacaaccttt ttaagtaatc caatttctaa actcctttac acacaatttt
    61021 acttcaagtt ttatagtcga aaatttatag taaacaagat gttatagtac gctcactgac
    61081 cttaaaagtc aataaacttt aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct
    61141 ttttcaaacg ctttaataca gcaaaaatct aacatttttt tatgggcttt ttctttgaat
    61201 tctataataa aaggatagaa cttgacttag aaaatcaagt tctatccttt ttgatatctt
    61261 aacttttgga agagagatgt aaggctgctt tctacccatc attcttacta ctcgaagcat
    61321 agcgcagttg tcacaacctc ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa
    61381 gcccaaccaa attaaaaaca taagaagctg tttttatttg gtcgttctta ttacttgatg
    61441 cagagcagct tttttacaac ctcttggtac acggtgttca atcagctgac cctcggtatt
    61501 agctcaaaaa aacggcaaaa tatgaggaac tatttttcca tttttttctc taatactcag
    61561 gtcaaaacac tgatttcaca acctcttttt ttaatgaaaa atatggctct catgcgccgc
    61621 ttctttgaat ttcgtcattt tttcttcgat gaacggatag attgccgttc ctacgattcc
    61681 aaagacaaca aacaaaccga tcatgatcca gattgctttc gtagcatttg gccaataaat
    61741 tcctcctgca gattcccgta acaagtttac cgcatgagta aatggcaaga acggattgat
    61801 catttggaag aatttccctg agacttggat cggatagttc ccgcctccgc cagagatcga
    61861 cagtactaag atgataatag cgatcccttt accgacatta tcgaataatg cagccaagac
    61921 atagacgatc atcatgaacg ctaaggcgat caagacagca aatgacacgc tgtagaccgg
    61981 attttggaca tctactccta gtaagaaaat attccctaat gtaacgatca agctttgcgc
    62041 aatcgccatt gtcaaaaatg tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt
    62101 gtttcggtct ttctttccta aatagtattc tgtcgtagca acacttgaaa gaagaacagc
    62161 gccgacccac agacatagtg cagtataaaa cggggtgcta gctgaaccgt tatttgcgat
    62221 tggataaagc gcattggttt gtagctcaac aggttgtgta aagaagtcac tttcttttgt
    62281 tgcgtctagt ttcaataatt tgatgacttc gcctagatcg acttcttgtt cgccttttcg
    62341 gatcgcgtct gctgcttttt ggataccagc tttgatattt ggccagtcat tgttgatcaa
    62401 gtccgcagca gcgttcacag ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa
    62461 tgtaccggtg atttcatttt ttagtgctgg ccaatcattt tggataaaat cagcggccaa
    62521 tcctaatttt tgctgaatga tcggtaattc attgttgtat aggtctgccc cttcattgat
    62581 gccattgacg atggtatcca tatttccatt cagcattgta ttggcatcat ggatttcttg
    62641 tccaattgca ggcatttctt tttgatattt ttctaaaatt gccactgcat tcgttacagt
    62701 tgctttcgtt gagctgagaa gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc
    62761 ttgtgcagtc ccaatcgtat cgatgacttg gtctagtaga gaagtgacag tggaactgat
    62821 ttgacctgta ttgataccag caatgatgcc attgatatta ttaacaacgg catcaatttg
    62881 atctagcaat tcatcaatag ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga
    62941 taggcggttt tgcagattgc ttagcaaggg ttttaattga tttagctgat caataattgg
    63001 ctgtaatttc gtatttccag cacttgtttg caatttagtc aggaactcga ccagtttatc
    63061 gattgcattt tgttgttgag atattccgtc aataaagccg ttcaatacat tgctgatgtt
    63121 tttcttttca tcatcagtca gcttaccatc ttcaatagct gttcttacag tatctattaa
    63181 cgaacctgtc gttgttgcca cttgtccaat tgacttcaat gtaacatcta cactgtttgt
    63241 gatgcttggt atcgcatctt tcagctgaga ggcacctgat tttgtttggt ttgctagatc
    63301 acttgcctga ttttctaaat ttttcacatc tggcaagata tcttgtactt gttggatgat
    63361 cgtcaagcct tgttttgctt catcgattcc ttggttcatt gtctcttgga tcgaagcaaa
    63421 atcgccgtcg atctctgcta gttgcttacc ggcattttgg atctctggga ttttttcttg
    63481 caaatcaagg atgatttttg cttgatcttt caactcaggc attttgtcat tcaatgcaac
    63541 gactttttca cccatctcat ctactttagg gatgtaatcc acgaattcat ttgctttatt
    63601 cagtttttcc ttgatttcag gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc
    63661 gtcgatcgtg tccaaatttt catctgttga taaaatcata tctttaactt tattgatact
    63721 tactaggttc gtatcgatgt cgtaacctat ctcattgaat actttcaata aagtcgaact
    63781 ggctgttttg ataaattcat ttgtgatttg cgattggatc gaacttgctc ctttatcggt
    63841 gattttcggt gcaatcgcat tgattttttc atttaccgta tattcgattt ttggtttctt
    63901 gatatcacca ctagtaaaac tcagcagatc ttctgagaaa tcctttggca agtaaatacc
    63961 tgcgtaatac tttccggaac ggactccgtc ttctaattgt tcttttgaat caacgaattg
    64021 ccatcctaat tgtttatttt tatgaagcga ttcgatgact tgctccccaa tggccacttc
    64081 tttaccttga aactctgctg gcttgtctgc actgtaaaca gcaattggca gctctccagt
    64141 atttccatat ggatcccata atgctttgat gttaaaccac gcatatagcg aagggatgat
    64201 catgatcgcg atgatcagaa gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag
    64261 caaatataat cgaagcgtat ttttgattga ctgcatagtt ctcccccttc ttcttttttc
    64321 agctgaatta ctttaccatg tatttcatac ttttttcaat cggctttttt caaaaaagtc
    64381 gaatttttgg acatatcacg gcaattgtct cattgtaatc tcttacatat tgagaaataa
    64441 ttaaggatta cagcctttac tttatcatga aataaaaggg aaagataatt ttatgccaaa
    64501 gtttttatct attatacgta tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac
    64561 aaaaatgttt cagctttttt cgttttttat tcctgtattt cttatttttc gaatgacagg
    64621 atgattttcc cagcaggagg atgtccttca actagctgat gtgcttggat gacatgctta
    64681 agtgaagcag acagattcat tgaaataaaa acatgaaagg ctcctttttg gtaggctttg
    64741 attatgccag caaaggcttc tgcatctaga tattcctttc ttggaacaaa cgattcataa
    64801 aagccttcct ttttttgccg caaatctaga tctgggaggt catttaatgc aacatacctg
    64861 ccgcctggtt tcatgatctg gatgccggtt tctcctttga tacttccttt cgtcgcatcg
    64921 attacaaggt ctgcctgatc agcaaattgc agacctggat ttgttttatc ataagcagca
    64981 aatgctgaag ctcctaattt ttttaccttt tcctcatttt tactggatgc actggttaag
    65041 attcggattc ccttttcatg gagaagctgg atcagacttg acccgaccgc acctgatgct
    65101 cctagtatca ttacagtatc tgtcggctgg atttccgtca aatggttgat tagattataa
    65161 gccgtaattc ctggcgtgac cattccggct gcttcttccc aactcatttt atctggaatt
    65221 tttgctactt ttgctgatgg caacacgact tcttctccgt aagtcccgcc gactgcatgg
    65281 acagctactc gatcacctac atgaacagtt gtgacatctg aaccaatctc tgtcacaata
    65341 ccagcgccat cattacctgg tacataaggg aatttcaatg ttcgtatctc tttcatttct
    65401 cctaaacgca aggcaacatc ataaggattc acactgaatg cttttatttc tacacgaaca
    65461 tgtccagaac cgacttctct gggatgagca tcgatttctt caaaaacatc ttgtgcttct
    65521 ccgtaaccgt taattgcaaa tcttcgcatg gtcaccatct ccctttttct ttaagtttac
    65581 gtttatgcct ttattttgac aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca
    65641 aaatagttgt cttttttcgc gtaacgtgtt aaattattta tctaaaagcg taaagattac
    65701 gatttacgct aataaaaaat aattggagga atttgttgat ggctaaaaaa tcaaagattg
    65761 ccaaagcaaa aaaacagcaa gaaacgattg aacgctatgc tgctttacgc caggaattaa
    65821 aagcgcaaaa agattacgaa ggcttgcgta aacttccttt agatgctcgt ccagaacgat
    65881 tgaaaaatcg tgatttaatc gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt
    65941 cccgtataaa attccgtgaa ttagcacata aaggtttgat tccaggagta aaaaaagcca
    66001 gctggtaatc caagtctggc tgtaatgtaa aggaggaggg cgcatggacc actccttgga
    66061 tcatcaaaaa agatacgttc gcttcttcta gcagaaatgc tggtactaga agcagacgta
    66121 tcttttttta cgcacagctg gtcctttttt atatgtaaag gctgcatggt tacttcgcta
    66181 tatttattta gtaacaggag tcagtgcttc gatacccaca ctaattttca atgctcggtc
    66241 aacttctgcc ataatgggtc gatccaaatg gcagaccttt tctttcaatc ttgatttatc
    66301 gatcgttctg atctgctcca acaggataac ggagtctctt tctattcctg ttccagtagc
    66361 tttgatccca atgtgagtcg gaagcttagg tttagccatt tttgccgtta tggccgcaac
    66421 aatgatcgtc gggctaaaat ggttacctag attattttga ataaccaaaa ctgggcgtgt
    66481 tccgccttgc tccgatccca cgactggaga aagatcagca aaataaatat ctcctctttt
    66541 taccatagga tcctccttat tcttgatatt ctcttggtat ccgctgtcct aatccgcaag
    66601 ccacttcata gtggatcgta cctaattgat ctgctatatc ctgcatagtg atttctttgt
    66661 ctgcattttt gccgataagc gtcacttttg ttccgacagg aaattcttga ggcaatcgga
    66721 tcatcagttg atccatacat acacgcccaa tcgtttcaca gtagttccct tctaccaata
    66781 gagaaaaacc ctgcatcttt cgtggccagc catctgcata accgatcggt atcgttccga
    66841 tccactctgc ttcaggagtg atatatgttt ctccatagcc gattccttca cctgctggga
    66901 gttttttgac ttgaatcaat tcagaaacca gctcaagtgc tggctgcaat ggataaactt
    66961 ccggtagtgc gtgcccagag ggattaagtc cgtacattgc tactccataa cgaatcatat
    67021 tgccgattgt ttcatcatgc cataaagccg ttgcactatt gctgacatgt acatatcgag
    67081 gaagttcggg aagttttttc agcacttcta taaagcgttc tttttgcaga ttccaatatg
    67141 tatcatctgc ctgatctgct gtagaaaaat gggtaaataa accttcccat tctaatacac
    67201 gactttcttt gatgaatgca gctgcctctt ttacttcttc aggtgtacaa aatccgatac
    67261 gacccatccc agtgtctgct tttagatgaa tcgaaagagg cgtctctagc ttacttttct
    67321 ctaaaacagc agctgctgct tgcaaccatt cccgtgtacc tgcagttact gataaatcat
    67381 gagcaacagc tacagtcagt gcgtcgtaag gaaccatatt caagataagg atgggctgag
    67441 taaatccagc ttctctcaac tcgactcctt catctaaatt tgaaacacaa aagcccgaag
    67501 ctccacctgc aactgctgcc tcagctgttt caatagctcc atgaccgtat ccgttcgctt
    67561 ttactactgc aaacaattct tttccttgcg gaagtcttgc aacttcattt gctacatttt
    67621 cagttatagc ttttttatgt atcactgctt tcgttgggcg gtgccaagcg acaaccatcg
    67681 tttttccttc tttctagtct tccaaaataa tttgtgcaaa agctgtttca tttgtatgag
    67741 taatactgac ccatacattg ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt
    67801 cattttttaa aatctcgaca tcttgaaagg taacttttcc gatcccagtt ccccaagctt
    67861 tagagaaagc ctctttgcaa gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag
    67921 gcaaggactg gaatagtttc atttcatcag aggtcaagat tcgtgcaatg aattttggtt
    67981 tttcttcgat cagccgagtg atcctaggaa gttcgactgc atcgatccca attcctttta
    68041 tcatatgctt ttctcttttc aataaattta gaacactctc attctaccaa attataacca
    68101 ttttgccaca aataagtcca caaataacaa aaatgaaagt ttctcttaaa aaagagaaac
    68161 tttcatttta tgtttaatcg ttatttgaac ggattacaaa tccgcgtttt ttatcattgt
    68221 gacgtttacc gccatctttt tggctgttat tatttttgct ataaccgcct tttgatttgt
    68281 ttccacgata attaccgccg tcacgacgat tacggttgtt tccgccacca cgtttgccat
    68341 ttttattgta tccttttttc tgcattggca atgggcgttc tggtgtgatc ttcactggca
    68401 ctgcatcagc tggatctttt gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag
    68461 aatattcttc taataattta tctgctgttt gcaggtattt atctagacca ttttctgcaa
    68521 gttttgtttc gatttgttca actgcagcac ctaattgacc tttgaatgct tctttttcgg
    68581 ttggtggacg taaagtcgtc atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt
    68641 ccatttcatt tggtgtaacg aaagtcacag acataccgcc ttttccggca cggcctgtac
    68701 gtccgatacg gtgaacgtag ctttctggat cttgagggat atcatagttg tacacatgag
    68761 tgacacctga aatatctaat ccacgtgcag caacgtctgt cgctacaagg atatctaaat
    68821 gaccgctctt aaatgaacgc aatacgctca ttcgtttttg ttgagacaaa tcgccatgga
    68881 ttccttctgc acggtaaccg cgtgcttcta atccgcgtgc caattcgtct acacggcgtt
    68941 ttgtacgtcc gaagacgatt gttaattcag gtgtttgaac atcaaacaag cgagtcatga
    69001 tatcgaattt ttcgtattct tttgcgcgga catagtattg atcgatcaag tcagctgtca
    69061 tttctttcgc tttgatcttc acatgatgtg gatttttcat gaatttaacg ccaatattct
    69121 tgatagctgg cggcatcgtt gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg
    69181 aaatgatttt ttcgatatct tccaagaagc ccatattcaa catttcatct gcttcatcca
    69241 aaactaatgt ttgaacggtt ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac
    69301 gtcctggtgt acccacaacg atatgtggac gatctttcaa gccacggatc tgacggccaa
    69361 tatctgctcc gccgtaaacg gcttgtacac gtactttttt gtcgcgccct aaacgatata
    69421 attcttcttg tgtttggata gctaattcac gtgttggtgc gataactaaa ccttgtaatt
    69481 cgtgacgatc tggatcgatt ttttctaaca ttggtaatcc aaatgcagct gttttccctg
    69541 tacctgtctg tgcttgtcca ataacgtctt ttccagctaa tgctagtgga atcgtttctg
    69601 cttggatagg cgttgcttct tcaaaaccag cgcgttcgac tgatttcaac aattctggtg
    69661 ataattctag ttctttaaat ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt
    69721 agcttttttg ctaccccgtc gatttgttcg gtcaaagtgt attgtttctt tttgacagtg
    69781 gccacgcacg tttttatcca ttcttagccc ctgtggcacc accgaaaaat cactttgcga
    69841 agtttaggca ttagaaaagc caaacgggtc gcgttcagct ttatcattat tatgacatct
    69901 ggcttcaaca agttgtttct atggcaaaac cagtccaatt tctcttcatc tttcggcaat
    69961 aggttattct aacatagttt ttcgttttct gcaagtttac agatttttat ttcatttgct
    70021 gaagttgttc taccacttca aataagccca tgctgttgct gccttttagc ataatactat
    70081 ctgttgcttc aagattttgt ttgatagcat caatcaattt attcttttct ttttcaaaat
    70141 agaacacagg tagttggggg tatttttggt tcaattcttc ttttagtgca tgcatttgtt
    70201 ccccataaag gaaaatcatg gcgaatttct caggatcgat atgttcactc atttgcgcat
    70261 gcatatttaa agactctttc cctaattcca gcatatctgc taacacagcg attctgcgtc
    70321 cttttgttgg cagattttta aaagtatcta ataccagtcc catggctgtt ggatttgcat
    70381 tgtagacatc gctcaaaata gctgcaccat ttcctgccgt tagccattcg gttctgtttt
    70441 gagtgataga aacatgggcg agcccttggc gtatctcagg tatggacagt ccaaagaagc
    70501 gcccgatcgt atacgcaatc aaagcattcg ttacattata acttccaggc agcgggatcg
    70561 taaatgtttc tccttctact acaaaatcgg tttgtgcttt tccttttgct gtcacttccg
    70621 catgaacatc gccatttcca ataccgaatg ttgtgactgt ttgtactaat gttgccgtta
    70681 aaggtgtcag caacggttca tctgcgggca caacgagtaa tccgtctgat acaagtccgt
    70741 cagtgatttc cattttggct ttagcgattc cttctcgcga cccaagattt tctatgtgtg
    70801 cttcaccgat cattgtgatt gctgcagctt ttggttgagc cataagtgaa agttccgtga
    70861 tttcattggc gtgatccatg cccatttcca aaacgatctt ttctgtatct tggggcatgt
    70921 gcaaaatcgt gtaaggaaga ccaatattat tgttgtaatt tccttgcgtc ttatatgttt
    70981 tgtatttttc agacaagaca gacgcggtta gatctttcgt ggtggtttta ccattgcttc
    71041 ctgtaattgc aacgacttct gcgcctactt tttccaagta ataggttgcc aattgctgca
    71101 tggctttcaa aggatctgct acaggaataa ctggaaaatt ttttggagct tgttcaacag
    71161 gcaagctcca aaatgaagca gatgctccgt ttgtgatcgc ttgctggata aattcatgtc
    71221 catctcgcgt accagcaaga ggaacgaata aacttccttc ggttatcttc cggctgtcaa
    71281 attcgacatt ggtaatcttt gtcagtgcag agcattcgct gcccacggct tgtgcaattt
    71341 cttgtattgt tagattcatt gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc
    71401 ctaaacggcg ggttcgcatg actctcactt ccaagtaaat ttctctattc gtatttagaa
    71461 gctagaatga acgcggttac gcaatcactc cagcctcttc tttatttctc tttattcatt
    71521 tttttcaaaa aaagattgac gctgatggta tcgattcatt cctaactgga tcagttcttc
    71581 aatcaaatca ccgtatttca agcccatatt ttcccataag agtgggtaca tactgaactc
    71641 cgtaaatcct ggcatagagt ttaattcatt caggaataat tcatttttat ttgtcaaaaa
    71701 gaaatcgcac cggctcaatc cgcttccacc taacatcgtg taagctaact tcgcgtactc
    71761 ttgcgctttt tgataaactt cttctggcac ttcggctgga atctgcattt cgattttatt
    71821 attgatatat tttgcttcat aatcatagaa tgctacgtct tttacgactt caccaggcaa
    71881 agtcgtccga acgtcttcat ttcctaatac agcaacttcg atttcgcgcg cttcaattcc
    71941 ttgttcaacg attgctcgag aatcatactg ataggctgtt gctaaagcat tttgcagctc
    72001 ttctcggttt tctgcttttg taatgccgac actagaaccc atattcgccg gtttgacaaa
    72061 catcggataa agcaaagaac cttcacattg atcaaatact tttttaggat tttctttcca
    72121 ttgattctta agtactggta cataaggaac ttgcggcaca ccagcagctt gtaaaatata
    72181 cttggtcatg attttatcca tggcacatgc actggtcaat acgcctgcgc cgacataagg
    72241 catattcaat gtctctaaga agccttggat cgttccatct tccccgtttg gcccatgtaa
    72301 aactggaaaa acgatggctc cttcttcttt gatttcgccc ggattgatca cttttcctgt
    72361 aaagccttcc tctgtctgtc cacttgggtc ccatgaaaga tgcaagacat ctttgctggc
    72421 aggtttttct gttagtaatg gacctttgac ccattgtcct tcttttgtaa taaatacgag
    72481 ttgaacttgg taataattat aataaatggc atttaaaact gaaaatgcgg aaagaatgga
    72541 cacttcatgc tctgcgctgc gtccgccata tagtaaagta atcttcaaaa gattgtcctc
    72601 ctgtattcta aatcgttcag tgatttactc tcatatttta gcacaaaaca aaaataaacg
    72661 aataacttat ttataaaaaa acgagccagc ttctaaaaga acgagcgtca tttatttttt
    72721 gtcagctaga ttatttgtcc cgttttttat aaaaagttat gataaagaaa aggagggatg
    72781 atgatggcgc aaacaaatcc acgcagccgc ttggcagaaa tcattcgagt gttcatcaat
    72841 tacaatgtag tgccaaactt tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt
    72901 gaagaactag gccccacctt catcaagata ggacagatgc tttctgttcg agaagatttg
    72961 ctttcttctg ccttcactca aacattcaaa acattacaag atagtgtacc tagcgacaca
    73021 ttttcaacgg tcaaaaagac gatcgaaaca gaactttctc tttcattatc agatatattc
    73081 gatgattttt caaaatcgcc ttttgcctct gcttctatgg ggcaggctca tcgtgccaag
    73141 ctgaagaacg gtgacgcggt agtcgttaag atccaacacc ctaacatcgc cgaagagatc
    73201 cgacttgatc ttcaactatt tgaacgagcg atccctttga tcaaatacat tcctgaaact
    73261 agcgtagttg atctaaaagg ggtactccaa gaagtaaaac gttccttgat caatgaaatg
    73321 gacttcttaa aagaaagtca aaatggcgaa caattctatc aaaagaacaa tggctggaaa
    73381 gagatccgca gccccaagat ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa
    73441 gaaatgtctg gtaaaaactt gaatcattta atgaacgccg aaaacaaaac tgaaactttt
    73501 atcactggaa ttcaaaacaa gcagctgaaa caagaagttg caaagctttt agttgagaac
    73561 tttatgaaac aggtgtttga tgatggtttt ttccatgctg atcctcatcc agggaatctg
    73621 ctattccatg tactaacaaa agaagaacag acacaagcta gccgaaaaac tgaaacagtc
    73681 catgaaaaag agttcggttc ctttgctttt cgagcctcca cttctgcaga agaccctgtt
    73741 gcgccatata ccatcaacta catcgatttt ggtatgatgg ggcatctttc tgcaggactt
    73801 aggcaaaaac tcacacaagc cgttttagcg ctgtatacga aagatgctta tcgaatcgaa
    73861 aaagcggtcc ttcgactttg tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa
    73921 gaactgacaa gttttcttga acagtattat gattctccta ttgatgaaat caacttgcaa
    73981 gaagtattca cacatgtcgt gacgatttgt catcaaaata atctgcagtt cgatcgtgat
    74041 atcacgcttt tgctgaaagc atttgggaca ttagaagggg tgatccgcgt cctcgatcct
    74101 gaagtttccc tcatggaagt cgcttcgcct tttgctcaac attattttct cactcatctg
    74161 gatgtagaag atacattgaa acaaagcgga ttagacttat tggaagggat gaaggctgcg
    74221 cctaagatcc ctcaacagct ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa
    74281 gtcaatttgg aattgaaaaa gcaagacaaa ttattgagca ggatcgaaag tatgatcaat
    74341 cggcttgttt tcggtatgat tttagctgca ttgatcgtag ggtcctccct gcttgtccaa
    74401 gcagcacctg tagaaaacgc agaggtcgtt tccttattag ggatatttac gtatgctatt
    74461 gccgcattcg tcattatttt tttggcaatc gatgcattga tccaaatgta taagaagagg
    74521 aaaaagtaga aaaaggttgt gacagaaaga atcagcttca agaaataaga aggggtttta
    74581 cataaattgt ttctcaaatt tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct
    74641 gaaacaccga gaataaaaaa tgtgaaaaag ctgttctgca cccagtaata agaacaatca
    74701 aaaagggaac atggaacaca tttatcctgt ttccctgttc cctttttgat gtattcgagc
    74761 ataaataaaa taccttcgtt acctactatc ttctgtactt ggtaaacctt agtcaattgc
    74821 cttttttaac gcttgacgaa aagacatatg atctttctcc gtaaagggtt ttggaccctt
    74881 tgtccttatg ccagcttttc ttaactttcc actatgataa cgttgttcta gaagcgtgcc
    74941 gatattcgct tcgtcaaatt cttttccaga gtgatggaac actcgcacac ccttcggcaa
    75001 tacaagtgaa gctaaaccat aatcttgcgt gatcaaaaag tcattcttct taaccatacc
    75061 gacgatttta taatccgcac tatcagctcc gcggtctacg tagacgattt ggacatgatt
    75121 agcgtattct ttggtcgtat aatggtccac actggtcacg ataaccacgt caagaccata
    75181 ggattctcct aattttatga catcttcttt tactggagaa ccatctccat caatgaatag
    75241 cctcattctt tttcgccttc ttccataaat gataaacagt tactaatccg ctcagtaaaa
    75301 gaccaacatt gatccatttt gccaagataa gcagttgtac actttctatc ggcactgatt
    75361 catggttcag cgtgatcaat aatagatgga ttggctctcc catttctttt gctaaaacaa
    75421 acaaaagcgt catcaataaa aactggtgga tcacactccc tttattttga gtagtatact
    75481 tgacaacgat ccggcgatcc attggaatag ctgtatcagt ttgataatat tccttattta
    75541 gccagctgcc tgttactacg tgccagaaac taagcagaaa gacacctatc gcaaaaccat
    75601 ttggaatcaa gaggaccaat aaagaagaac tcagtccaag caattgcata gccaatggag
    75661 accatgttaa acgtttcaaa atccatggtc cgactatttt tgctgcaatc aagcctgctg
    75721 cataaggcaa atacataaaa atcgaaagtt tttctaatcc gtacagtatg ccaacataaa
    75781 aactgctaaa taaaaaaagg aaattcccta gcattccatt tagaaaagta aacagattta
    75841 gccagcttgg caacttccat tccttcttct ggtgtcctaa aacaaacacg aacaagatga
    75901 acagcagcaa gaaaccataa atagcaagat caaaaagcct ttcatcgaat aacaatcggg
    75961 catttctcaa aagaaacaat aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag
    76021 ctaccagatt tcttttgatg tctttgaaat ccagttcgta acgcggataa tgactaaccg
    76081 tatggtaggc catgacgtaa aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg
    76141 gtgcaggcaa ctcaatcgct tgatacagga gaaatagcca gatagctgca aataagtact
    76201 ttttcccatc catattgata aaaccttgcg tcttttcgtg aaaattgacc gtggtattag
    76261 caggtacaag ccatgctgca ctcagcccta gcaaaacact tgaaaaataa aataaaggaa
    76321 aataaaatac gcctaatatt ccaagcaatg aaccagcacc accaatcagc agagcgatca
    76381 tcagcaacgt gtaactatct agaccaaatc gtagactgtt gactaaaaat agtccagtca
    76441 tccgaaaggc ataaaacagc gtaaatggca aaatcgtcag ccaattatgc gagtctgtta
    76501 gtcccaaaaa taaaagataa ggaataaaaa taaccgtgtt acttaagata aagggcgttg
    76561 tttgtgtaaa attacgccca aatcgtttga gtttttcgaa catgacaatg gctccttaaa
    76621 cagtcttata actaatctgc tcttcgattt tagcattaaa ggggaaataa accaatttta
    76681 cagaatatat tcttgtaaaa aaaaatacag aaagaagcat aagcttcttt ctgcattttt
    76741 acggtacttg attactcaag cagacaagcc cttctccaca aaagtcttgc ccgacacccc
    76801 tgcatccggc aggtgataaa gatagaaatc aattatcctg tgcctccaaa atcaattagg
    76861 agctctgtaa accagagtcc ttctcgaccc gtcgcataaa tttactttaa cacagttcct
    76921 atgtttatgc gagagctttt ttcattttat aatcgaccat ttgaaatcct tgaccgatat
    76981 ctgaaataag ttcttctgta atctcaaatc cttcatgctt ataaacctcg attgcttgat
    77041 gattcccctg attcactcgc aaaaagatct ctctttcctt tgtttgcgca gcaagttctt
    77101 cataccagcg aaaaactgaa gaagtcaatc cttgaccacg tactttattt agcaaatata
    77161 atttacttaa ataaattttt ccatttatct cttcataagc agtgtaaccg acagtctttt
    77221 cttcgctttt taataaaaag taggacacac cttctgtcag ttctttttga atctgttttt
    77281 ttgactgata agttttcagc atgtaatcca cttgtttttg accaatgatc ggcacaaaaa
    77341 cttccggcca tacttcttgt atgactgcca tcagctcatc cagttcattt tctgttactt
    77401 ttattttttc catacaccca cgtccttttt tacattataa atgaccttgc gcttgatgga
    77461 ataaaaagag catgatcatg ctatacacac ccaccgacca cggtttcaag aaaatcacaa
    77521 tctgcataaa ttccctaaaa gataatttcg tcaaaccagc tactagacag atcaagtcgt
    77581 ctggcgcaaa gggaagcagc aaggtaacaa ctagtacttt tttaatatct ttcgtttttc
    77641 ttgtcagcaa ttcatcaaat ttctgatact tttttggcga taaaataagc tggacaaagg
    77701 ctacgccata gtaacggaca agcaaaaatc cgattgcttc cccaatcact aggccaatac
    77761 aactatataa aagcccaaat ccattgccaa acatcagcat gcccgctact gaggagatcc
    77821 ctcccggcaa aataggaatg atcacttgga tcacttgcaa cagaacaaat atcatcactg
    77881 catacttgcc aaattgctgg ataaacgctt gcaaagacgt gctcgatcga aaaatcccat
    77941 gatgataagc ataaagaatc aacacaaaga aaaaaaacag accaataatg ggcatgatct
    78001 gaagacattt ttgtaaaact gatccttttt tagacatcta tattccccac atcttttttc
    78061 atttattaaa actgcctcct
//
LOCUS       DANJMI010000006        76490 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000006 DANJMI010000000
VERSION     DANJMI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.006"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..420
                     /locus_tag="PWU81_000500"
     CDS             <1..420
                     /locus_tag="PWU81_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDL1109234.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(504..1226)
                     /locus_tag="PWU81_000501"
     CDS             complement(504..1226)
                     /locus_tag="PWU81_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL1109235.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(1216..1953)
                     /locus_tag="PWU81_000502"
     CDS             complement(1216..1953)
                     /locus_tag="PWU81_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1109236.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1965..2957)
                     /locus_tag="PWU81_000503"
     CDS             complement(1965..2957)
                     /locus_tag="PWU81_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1109237.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2972..3715)
                     /locus_tag="PWU81_000504"
     CDS             complement(2972..3715)
                     /locus_tag="PWU81_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1109238.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3789..3893)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3956..5278)
                     /locus_tag="PWU81_000505"
     CDS             complement(3956..5278)
                     /locus_tag="PWU81_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL1109239.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(5265..6230)
                     /locus_tag="PWU81_000506"
     CDS             complement(5265..6230)
                     /locus_tag="PWU81_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1109240.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(6227..7765)
                     /locus_tag="PWU81_000507"
     CDS             complement(6227..7765)
                     /locus_tag="PWU81_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDL1109241.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(8181..8468)
                     /locus_tag="PWU81_000508"
     CDS             complement(8181..8468)
                     /locus_tag="PWU81_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109242.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8602..9270
                     /locus_tag="PWU81_000509"
     CDS             8602..9270
                     /locus_tag="PWU81_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL1109243.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9561..10301)
                     /locus_tag="PWU81_000510"
     CDS             complement(9561..10301)
                     /locus_tag="PWU81_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109244.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10431..11378)
                     /locus_tag="PWU81_000511"
     CDS             complement(10431..11378)
                     /locus_tag="PWU81_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1109245.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11507..12271)
                     /locus_tag="PWU81_000512"
     CDS             complement(11507..12271)
                     /locus_tag="PWU81_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL1109246.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            complement(12275..12664)
                     /locus_tag="PWU81_000513"
     CDS             complement(12275..12664)
                     /locus_tag="PWU81_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109247.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12799..13341
                     /locus_tag="PWU81_000514"
     CDS             12799..13341
                     /locus_tag="PWU81_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDL1109248.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13489..14121
                     /locus_tag="PWU81_000515"
     CDS             13489..14121
                     /locus_tag="PWU81_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109249.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(14255..14380)
                     /locus_tag="PWU81_000516"
     CDS             complement(14255..14380)
                     /locus_tag="PWU81_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1109250.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(14514..15407)
                     /locus_tag="PWU81_000517"
     CDS             complement(14514..15407)
                     /locus_tag="PWU81_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HDL1109251.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(15589..16515)
                     /locus_tag="PWU81_000518"
     CDS             complement(15589..16515)
                     /locus_tag="PWU81_000518"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDL1109252.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="PWU81_000519"
     CDS             complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="PWU81_000519"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDL1109253.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="PWU81_000520"
     CDS             complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="PWU81_000520"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL1109254.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(20113..22563)
                     /gene="parC"
                     /locus_tag="PWU81_000521"
     CDS             complement(20113..22563)
                     /gene="parC"
                     /locus_tag="PWU81_000521"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL1109255.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22575..24620)
                     /gene="parE"
                     /locus_tag="PWU81_000522"
     CDS             complement(22575..24620)
                     /gene="parE"
                     /locus_tag="PWU81_000522"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL1109256.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24786..25220)
                     /locus_tag="PWU81_000523"
     CDS             complement(24786..25220)
                     /locus_tag="PWU81_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDL1109257.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25347..25985
                     /gene="plsY"
                     /locus_tag="PWU81_000524"
     CDS             25347..25985
                     /gene="plsY"
                     /locus_tag="PWU81_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL1109258.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(26117..26992)
                     /locus_tag="PWU81_000525"
     CDS             complement(26117..26992)
                     /locus_tag="PWU81_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDL1109259.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27074..27865)
                     /gene="codY"
                     /locus_tag="PWU81_000526"
     CDS             complement(27074..27865)
                     /gene="codY"
                     /locus_tag="PWU81_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDL1109260.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="PWU81_000527"
     CDS             complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="PWU81_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDL1109261.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="PWU81_000528"
     CDS             complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="PWU81_000528"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HDL1109262.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="PWU81_000529"
     CDS             complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="PWU81_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL1109263.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="PWU81_000530"
     CDS             complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="PWU81_000530"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HDL1109264.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(32330..34408)
                     /gene="topA"
                     /locus_tag="PWU81_000531"
     CDS             complement(32330..34408)
                     /gene="topA"
                     /locus_tag="PWU81_000531"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL1109265.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="PWU81_000532"
     CDS             complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="PWU81_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL1109266.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(35443..36210)
                     /locus_tag="PWU81_000533"
     CDS             complement(35443..36210)
                     /locus_tag="PWU81_000533"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL1109267.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="PWU81_000534"
     CDS             complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="PWU81_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDL1109268.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            37323..37721
                     /locus_tag="PWU81_000535"
     CDS             37323..37721
                     /locus_tag="PWU81_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1109269.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            38000..38500
                     /locus_tag="PWU81_000536"
     CDS             38000..38500
                     /locus_tag="PWU81_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL1109270.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(38583..39134)
                     /gene="lepB"
                     /locus_tag="PWU81_000537"
     CDS             complement(38583..39134)
                     /gene="lepB"
                     /locus_tag="PWU81_000537"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL1109271.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(39286..39513)
                     /locus_tag="PWU81_000538"
     CDS             complement(39286..39513)
                     /locus_tag="PWU81_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDL1109272.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(39510..40028)
                     /gene="msrA"
                     /locus_tag="PWU81_000539"
     CDS             complement(39510..40028)
                     /gene="msrA"
                     /locus_tag="PWU81_000539"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL1109273.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(40048..40761)
                     /locus_tag="PWU81_000540"
     CDS             complement(40048..40761)
                     /locus_tag="PWU81_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HDL1109274.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(40764..41660)
                     /locus_tag="PWU81_000541"
     CDS             complement(40764..41660)
                     /locus_tag="PWU81_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL1109275.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(41818..42660)
                     /locus_tag="PWU81_000542"
     CDS             complement(41818..42660)
                     /locus_tag="PWU81_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL1109276.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            42792..43445
                     /locus_tag="PWU81_000543"
     CDS             42792..43445
                     /locus_tag="PWU81_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDL1109277.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(43508..44035)
                     /locus_tag="PWU81_000544"
     CDS             complement(43508..44035)
                     /locus_tag="PWU81_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL1109278.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(44055..45002)
                     /locus_tag="PWU81_000545"
     CDS             complement(44055..45002)
                     /locus_tag="PWU81_000545"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL1109279.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(45014..46900)
                     /locus_tag="PWU81_000546"
     CDS             complement(45014..46900)
                     /locus_tag="PWU81_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL1109280.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(47060..49138)
                     /locus_tag="PWU81_000547"
     CDS             complement(47060..49138)
                     /locus_tag="PWU81_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL1109281.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(49151..49426)
                     /locus_tag="PWU81_000548"
     CDS             complement(49151..49426)
                     /locus_tag="PWU81_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109282.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(49788..50243)
                     /locus_tag="PWU81_000549"
     CDS             complement(49788..50243)
                     /locus_tag="PWU81_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109283.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(50397..51605)
                     /locus_tag="PWU81_000550"
     CDS             complement(50397..51605)
                     /locus_tag="PWU81_000550"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDL1109284.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(51717..52049)
                     /locus_tag="PWU81_000551"
     CDS             complement(51717..52049)
                     /locus_tag="PWU81_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL1109285.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            52197..53072
                     /locus_tag="PWU81_000552"
     CDS             52197..53072
                     /locus_tag="PWU81_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL1109286.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(53154..53735)
                     /locus_tag="PWU81_000553"
     CDS             complement(53154..53735)
                     /locus_tag="PWU81_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDL1109287.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(53741..55000)
                     /locus_tag="PWU81_000554"
     CDS             complement(53741..55000)
                     /locus_tag="PWU81_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL1109288.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(55002..56048)
                     /locus_tag="PWU81_000555"
     CDS             complement(55002..56048)
                     /locus_tag="PWU81_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109289.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(56283..56558)
                     /locus_tag="PWU81_000556"
     CDS             complement(56283..56558)
                     /locus_tag="PWU81_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL1109290.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(56801..58111)
                     /gene="der"
                     /locus_tag="PWU81_000557"
     CDS             complement(56801..58111)
                     /gene="der"
                     /locus_tag="PWU81_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL1109291.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(58293..59522)
                     /gene="rpsA"
                     /locus_tag="PWU81_000558"
     CDS             complement(58293..59522)
                     /gene="rpsA"
                     /locus_tag="PWU81_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL1109292.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(59657..60337)
                     /locus_tag="PWU81_000559"
     CDS             complement(59657..60337)
                     /locus_tag="PWU81_000559"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL1109293.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(60386..61021)
                     /locus_tag="PWU81_000560"
     CDS             complement(60386..61021)
                     /locus_tag="PWU81_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL1109294.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(61087..62490)
                     /locus_tag="PWU81_000561"
     CDS             complement(61087..62490)
                     /locus_tag="PWU81_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDL1109295.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(62477..63508)
                     /locus_tag="PWU81_000562"
     CDS             complement(62477..63508)
                     /locus_tag="PWU81_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1109296.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            63539..63760
                     /locus_tag="PWU81_000563"
     CDS             63539..63760
                     /locus_tag="PWU81_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDL1109297.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(63925..64662)
                     /locus_tag="PWU81_000564"
     CDS             complement(63925..64662)
                     /locus_tag="PWU81_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HDL1109298.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(64815..66155)
                     /locus_tag="PWU81_000565"
     CDS             complement(64815..66155)
                     /locus_tag="PWU81_000565"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDL1109299.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(66392..67915)
                     /locus_tag="PWU81_000566"
     CDS             complement(66392..67915)
                     /locus_tag="PWU81_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDL1109300.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(68293..68904)
                     /locus_tag="PWU81_000567"
     CDS             complement(68293..68904)
                     /locus_tag="PWU81_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL1109301.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(69027..69152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(69234..69950)
                     /locus_tag="PWU81_000568"
     CDS             complement(69234..69950)
                     /locus_tag="PWU81_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL1109302.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(69956..70525)
                     /gene="scpB"
                     /locus_tag="PWU81_000569"
     CDS             complement(69956..70525)
                     /gene="scpB"
                     /locus_tag="PWU81_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDL1109303.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(70509..71306)
                     /locus_tag="PWU81_000570"
     CDS             complement(70509..71306)
                     /locus_tag="PWU81_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDL1109304.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(71276..71647)
                     /locus_tag="PWU81_000571"
     CDS             complement(71276..71647)
                     /locus_tag="PWU81_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL1109305.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(71666..72553)
                     /gene="xerD"
                     /locus_tag="PWU81_000572"
     CDS             complement(71666..72553)
                     /gene="xerD"
                     /locus_tag="PWU81_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL1109306.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(72649..73122)
                     /locus_tag="PWU81_000573"
     CDS             complement(72649..73122)
                     /locus_tag="PWU81_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL1109307.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(73227..74090)
                     /locus_tag="PWU81_000574"
     CDS             complement(73227..74090)
                     /locus_tag="PWU81_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL1109308.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(74286..76385)
                     /locus_tag="PWU81_000575"
     CDS             complement(74286..76385)
                     /locus_tag="PWU81_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL1109309.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT        20953 a        16098 c        13038 g        26401 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacaac ctgttgtaca agaaacggtt
      121 caaacggaaa aacaagcagc gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa
      181 actgctgcta ctccagcaag cacaagctct gcaaaagaat ggatcgcaca aaaagaatca
      241 agcggttctt acacagcaac gaacggacgt tacatcggac gttaccaatt agattcttct
      301 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
      361 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      421 gattcattta ttaatcaaac agtttacttt tatacaaaga aagccaaaaa gaggtgcgac
      481 actagttgtg tcacatctct tttttattta cctaatctaa tgaaacgttc caagttttga
      541 taagtatttt ttttaacttc ttcaactgat agctctttga tctcagcgat tgtctttaac
      601 tgttcttcaa gcagtgagac ataatctttt tctgaaaaaa tcccccctaa agcccactct
      661 gctgcatgta gtccatccgt ttcaataagt aagtggtcga ttgggactgc tttagctagt
      721 tgccagactg ctggattctt tttcaaatca agaccaatgc tgaaataaca gcctaaatca
      781 atatagtctt gttgatagtc caaagaagaa taccaatgga tcaaatagtg attagggtaa
      841 gcttggatat aatttaagat ctctttttcg catccttttg tgtgcagtac aacaggtttc
      901 tcatttatgg ctgcaaaagc aagctgtcgc tcaaatactt ttttttgtgt agtcatcggt
      961 acattcgtcc aaatattatc taaccctatt tcaccgatga tccttgcttt tttcaaaaaa
     1021 ggctcaactt gttcaaacgt atagctatct gctttccaag gatggatacc gtaacttaag
     1081 gcttgagtct tacttgctgc aagctgtctg ttttccttcc attcttctgg cgaatcacaa
     1141 ttgatgatcg tcaaaatcgt gtgttcctgc tgaaggagtg ctaaactctg actcccatct
     1201 aaatgacaat gagcatcact taacatctaa ttcacctgcc aataactgat aaatcgtttg
     1261 cttcaactct gattgctgca gtagccattc acgtgaccgt cgtttttcag gcacttgaat
     1321 ctcagcaatc acatgtgccg gacgggcgga aagcacaaga atcctgtctg ataaataaat
     1381 tgcttcttcg atatcatgcg tcacaagtaa agtagtccgc tttgcttttt taggcagctt
     1441 caacatccag tcttgcatct gccctctggt catagcatct aacgctccaa atggttcatc
     1501 aagaagcaag taagaagaag gattcatgat tgttcgtaac aaggccgttc gctgcttcat
     1561 cccgccagat aattgttccg gcaaaaaatg ttcatagccg tttaacccaa agatatccag
     1621 ataatccctt acttgttctt cattgattgt tagatgattg attttttgat ataacgtaac
     1681 gttttgatag acgctcagcc aaggaagtaa catatcttct tgcggcatat aactaaatgc
     1741 tgtatggaga ccagttactt tttgatcgtc tactttgatc gttccagaag tcataggctg
     1801 gactcctgtt aaagttttca gcaatgttga cttaccggct ccgcttggcg cgacaatcga
     1861 tgtaaaggta tctttaggga tagtgaaatg gatgtcttct aatattttca ggttttcaat
     1921 gactacattc aagtggctga tttggattga catatctatc actcctattt tataaactga
     1981 ttagtgaata gttcttgcga aggtacatct tgcttgatca atccgttttc ctgcataaaa
     2041 gctgtataat tgcgccaaac tttttcttcc atcaatcccc aattctcagg atcatccgta
     2101 taattttttg acaagaatgc ttgtgattct tctaaaaacg tccgatcatt ttcttttgca
     2161 tatttttgca acagtttagc actgtcattt ggatccttaa tagcttcctg atagcctttt
     2221 ttcgtggcat ccataaaaga ctgtaccaat tctggatcac ttttgatcag ttgatcattc
     2281 gtaataatta ccggagtata gtaatctaac cgttcatcta attctttcaa tgggatatag
     2341 tttagttcaa tacccgcttc ttttgctttg atcatatccc aaccttcaaa gtaccattgg
     2401 atatctgagg attttcctaa gctttctggc ccttctccat tgcttcctac cattgtcagt
     2461 tttgaaaagt ccccgccgtc tttttccatc acagctttta agacagcttc ttcacttgga
     2521 ctttgccaac ctgcatatgt ttttccttca aaatcttttg gtgaatggat gttactgtct
     2581 gatagggtgg cgaatccaga agtattatgt ttgatcactg tagcaattgc tttgactggt
     2641 atattctgtc catctgcgtg agcataagtg acatcttctt gataacttac accaaattgt
     2701 gctttgtccg cacctactag accaatactt gtcgaattat ctcctggttc gatgatttgg
     2761 acatttaaac cagcctcttt ataatagcct ttttcttttg ctaggtagat gccagtatga
     2821 ttagtattcg gtacataatc caaaacaagt gtcacttttt tcaaatcctt tatttcttgt
     2881 ttgctacttg tttcttttgc agtgccacaa gcggataaaa gcaacagtag cggtaataaa
     2941 agccataatt ttttcatttg atccccttct cttaaaaaat ataaagcata cgataatacc
     3001 cttttttcaa taatgaacaa gcgccattca gcaataaact caatagaatg acacaaatga
     3061 tcgcagcaaa aactttatcg atctggtagc tgttttttac gcgtatcatg taatacccta
     3121 agcctgcttg agcgcttagc cattctccaa cgactgctcc gctgacacaa taagtagcag
     3181 ccacttttaa accggaaaag aagccggata aacccgctgg tattttatat attcgataaa
     3241 tcttccctgt atctgccccc atcgttttca aaaagatgat ttgctctttt gatactttcc
     3301 tcaatgtatc cgaaaaagaa actacgatcg gaaaatagct tatcaagaca acaaggatca
     3361 cttttggaag cataccgaaa ccaaaccata gggtaagtaa tggccctaac accatgattg
     3421 gcaacatttg tgaaatcact agtaacggat aaaaagacat ttctaaaaac ttccaataat
     3481 ccattgccaa ggcagtaata aaagaaagaa cgctagctat taccaaacca agcactgctt
     3541 catttaatgt cgtgacagaa tgggaaagta atacttgtgc atctgccgct aatgcagaaa
     3601 atacagcagc aggtccgggt aaaataaagg caggtatttt ccaaactatg acaacaatct
     3661 gccaaataat taaaatgaat gccatactga ctgctggata aagatttttc ttcattttgt
     3721 tacctccttt cttctaatat gaataacaaa aaacgcttga aatgggcacg acatagtaac
     3781 ccgcttcaag cgtctgctat gtttctccct tcaccagtat taactgacag gttcaatggg
     3841 tctaagttca caacttactc tcatgtcgtc tacgacatcc ccagaaacag gtattcattt
     3901 tttaaagtgt agcacaagaa ggaaaaagca acaagcgctt tagttatcat gctgattatt
     3961 tcaataaata ggagagatca cttgccaatg tcggataccc catgatatag ctatccaact
     4021 cttttttagt caatttttga ttgatgacga gtgtcaaatc atcgatcaat tcgtctgctt
     4081 gctcgctaat cactgcagca ccaatcaaat aattttgctg gtcaaaggtc aattcagcct
     4141 tagcaactgg ttcattgaca cgatgatagg tgaaccaact ggtaagatcc atctctacca
     4201 cttgatctga cgaagaggcg tgactttttg taactcctac ttcagcaagt ttaggactgg
     4261 cgtagacgat cgtaggtata atcggatatt tgataggttc gcttgttgca tccgttatcc
     4321 gcttagctac ataatttcct tcaaaagtgg cgactggtgt caattttggg gttttccttg
     4381 aaacaatatc tccacacgca aaaatttttg gattgctcgt ttgcagataa tcattgactg
     4441 caattccgtg tttatcaaag acgatattcg cttgctcaag tgctaaagat tctgtattcg
     4501 gctgtctccc agtcgcacaa aaaatcatat ctgctactaa ttccgtctct tttccgacta
     4561 aacgatagcg agtaccttct gaaatgatct tttgggtttc aacaccaaaa gcaaactgaa
     4621 tgccagatgc ttccatttga tggacggctt cctctaccaa agacgcctca aattctttta
     4681 atggccgttg attatgatgg acaatggtta ctttgctccc tgcagcattg gcaatcgtag
     4741 ctagctcaaa agcaatataa ccaccaccga taaaaatgat ttctttaggc agtacaggca
     4801 atgataaaaa gtctgcacta gttttcaagt attcctttcc ctccactggt aagatcgtag
     4861 gtctttgccc ggttgccaaa actaagtaat ccgcatggaa gacttcttcg ttgacttcaa
     4921 tagatgaatc atccaaaaaa cgagcggttc ctgaaagatg atcgatttca gcttctgcta
     4981 attgtttttt tcggctttct gggactggat ccgtaaatgt tcgtttgaat ttttgaagtt
     5041 cttcccagtt ggcagttggg atctcgttaa aaccttttcc actcaattgt tttacccgat
     5101 tacgtgcctc tactgcactt aacaagactt ttttaggatc acatccacgg ttaggacaag
     5161 tcccgcccca tagatcttct tcgaccacta gtactgtttt acctgcttct ttcaatccat
     5221 aagcagcact taagccgctc acacctgaac cgatgatgat tgcatcatat gttttcattt
     5281 ccttcctcct ctcgacttaa aaaagcaaag atttctttac ttgtcagcga cccaatactt
     5341 ctttgctttt ctactatcac aatgcttgga tacaccgaaa gaagcgaata tacttctttc
     5401 ataggcgtat caaagcaaac ttgttgacga gcattttctg gtatttcatt taagaaatat
     5461 ccattgacga ctaaatcttc tactcgccac tctttcgtct gtttcggttc atcaaagaaa
     5521 tgccgaacgt agtcgttttt aggattcatc aataactgct ctggtgtgtc acattggata
     5581 atttctccat ccttcataaa tgcaatacga tcacctagct tgattgcttc ttccatattg
     5641 tgcgtaacga aaacgatggt cgtgcccaat tcttcgtgta aggacaaaac caattcttgt
     5701 aatgagtttc ttgaaatcgg atctaatgcg ctaaatggct cgtccatcaa aatcacatct
     5761 gggcttgcag caatagctcg gatgatcccg atccgttgct gttctcctcc agacaactct
     5821 ctaggcatac gatctcgata aatatcagga gataagccaa ctttttgaag aagttcatct
     5881 acacggtctg ctcttttctg cttttcccaa cctaacattt cagggatgac ttcaatattt
     5941 tgcttgactg tcatggtcgg gaacaaagcg atttgctgca gcacatatcc catattccac
     6001 cgcattttct gcagattata ttcatttaac gattttcctt tgaagtagat gtctcctgct
     6061 gatggtttag ataatccatt gatcattttc aaagtagttg tttttccact accggaagga
     6121 ccaaccaaaa caaagatttc tccttttttg attgccagat ttaggtcatc gatgacatac
     6181 tttccatcat actttttttg cacatgtttg aactcgatta atttagtcac tgatcttcct
     6241 cctttactaa tccttcattt ttcaggaaga ttcgtgctac ttctgtcggc tttttctttt
     6301 cgacattgac ttggtaattc atatcaatca tttgatcctc tgttattttt ccgcttaatt
     6361 tattcagtgc tttaacgatt tcaggatgtt ctttagcaaa atcttttttc atcagcggtg
     6421 cgccttgata agctgggaat aaccctcgat catcttctag agtcactaga tcgtactcct
     6481 tgagctggct atctgtggaa taggcatcca caatattgac atctccatta ttgattgctt
     6541 ggtaacgcaa acttggctcc atgctttgta cacttgaaaa attcaagcca tatgcttctt
     6601 ggattccttt atatccatct gaacgatcaa taaattccag tgtgaatccc gctttgatct
     6661 gatcagagat agctgccaaa tcggaaatct ttttaagttg atgttcgttc gcaaaagatc
     6721 tcttgactgc caaggcatac gtattttggt aagccattgg ttttaataat gtcatatcga
     6781 attgcttatt caataattct tctgcttcgt catatgtttt ttcttttgtc aaatcagcac
     6841 ttttcattgc ttcaggtacc ttcaccaagc tttctaggac agtacctgtg aactcaggat
     6901 agatatcgat ttgaccttga tccagtgcat tgaacaaaaa actcgtcttg ccaaaattcg
     6961 gttttaattc aacttctgtt tttgtgttgt cttcaatcaa ttctttatac atttggatca
     7021 ggatttcagg ttccgctcct aattttccag caatcgtgat ggtttcattt ttaaacaagg
     7081 cagactgata taaagaaacg ccgccaaata aacacaccac tgctgctaaa gtaatgatgg
     7141 aatatcgagg tttcttatgc tgcaaccagc gaatcagtcc gctgaatata attgcaagca
     7201 aggcagaact gatcgcacca atcaatgtca aaacaggtgt atttcggtca atacctaaca
     7261 aaataaatgt ccctaatcca cctgctccga tcaatgctgc caaagtagcc gtaccgatga
     7321 ttaaaactaa ggcagttcgg atacctgaga taatgacagg taatgcgatg ggaagctcta
     7381 ctttcagcaa gcgtctcatt ctggacatac caaatgctac cgctgcttct tcaatagacg
     7441 ggtcaatttc agctagacca atatacgtgt tttggaaaat cggaagcaaa gcgtaaataa
     7501 caagtgcaat caatgctgga acagttccga tccctacaaa tgggataagt aaacctaata
     7561 aggcaagtga aggaatcgtt tggagcacac ccgtgatctg caataagact tctgccattt
     7621 ttttatgatt ggctgcccaa atcgctaaag gaatagcaat cagtgctgca atcagcaaag
     7681 aaatgagaga aatggacaga tgttgaaatg cagcttccag caactcgcct tttctttcac
     7741 ttaatgtttg tataaaatta ttcatgcgac cttcctttct gttattcttt aagtataaac
     7801 atttctttcc tttttcccaa ctaacttgtc ttgccttaac cttacagcac gaacaaaaaa
     7861 aagaaaaaaa tacctctaaa tatgctgaca aaaaaagaat ccaagcaaca aagtcggatc
     7921 aactttttgg ttctcgtttt ttcactacga ataccataaa aatctatacg aaattgtcac
     7981 ttggaatcag ctatatcttt tttacacaca caacttttat tttaataaaa agaaacagcc
     8041 aataaagtag aaatctcgtg ggggagaaca cctttattgg cctgttcaac gaatattata
     8101 ctgtatcatg acactttttt gccaaaaatg taaaaaatgg ttgttttgac atcataaacg
     8161 tcttgttttc ttatttttac ttatatcacc actttatttt ctatctcttt cactccataa
     8221 agaggaatga gttcttcttt tgactgataa taaacagctt tctcacggtt cttcatcaaa
     8281 aaaagaaaga tgtctttcat agcaagtggt gctttgccgt gttcaaataa aaagataaat
     8341 tgatttgctt tatttatctg gaccattgtc acaggcacca gtttttgttc ttgagccaaa
     8401 aaaactcctt gatactctgt ttttttgggt gattttgtaa gaattgtcaa taagtctgct
     8461 gctcgcattt ttccactcct tatttgataa ataaaaagaa acagccaaaa ataatttttt
     8521 ttcattttcc tcttgaagta agagtataag tcagtataat aaagaagttg ctgaaaattt
     8581 tcaaaagaaa agaggctaga aatgaaaaaa ttaaatttat ttacagaatt gtgcggaatc
     8641 attttaggcg caggaatcta cggattagct gtgacaggaa tcaatattcc atctaagctg
     8701 gcagatggtg gggttactgg tattgctttg cttttgcata atctttttgg attcgctcct
     8761 tctgtcactt cactgctttt taatttacct ttattgctta tctcattgtt tctttttgga
     8821 aaacacgctt ttctccggac gatcgtcgga acatttgcgc tagttttctt tttacattta
     8881 tgggagagcc ttcatataca ttttgctgta gatagtctac tgatcaatag tttgatgaca
     8941 ggtattcttt ctggcattgg ttgcggtctc gtctttcgat tcggcggaag cacaggcgga
     9001 acagatatca tttatcaagc cattgaaaaa tattttcata ttaagattgg caaaagtctt
     9061 ttcttgatta cttttggtat tttagttgtc tccttgcttt atttggatct cacacatttt
     9121 ctgtatacat tacttagctg ttctattcta tcttatacat tgaataaagt gaagtatctg
     9181 gaaattggat ctgttttgcc ccaattggaa gcaaaacaga ctacagaaac atttgaacca
     9241 cttgatcctc ttgaacagtt agagcactga ttcttatttt tcactacttt atttaattta
     9301 cagaaatatc attatgtaaa ctaaaatgat attattcagt aaaatcttct ttataaaaaa
     9361 ataaactatt tttgcaaacg atctgcgaaa atagtttgtt tttttatttt gccattattt
     9421 tcttacttat tgggagaaaa aattcgctta aactcaaaaa aatttgagga tacgatataa
     9481 actttcggat agagtataaa ataaggaaac gccgcagcgt ttcctttcca actgtagccg
     9541 aatataaatt tgtatatcac ttatttaaaa tagctatttt ctcccaaaga gtttccttga
     9601 tcatcgatca atccttgtaa tgagcgttct aagataccat ggcttttttt cgattttttt
     9661 tcttctgact gctttgtttc ctctaacgtt tttgttcgat tgaatgttcg tacagacggc
     9721 tcaccatttc ttttttcttg atacggcgtc ggttctgtat cgaacaacag ataagagtca
     9781 tccgtttttt tcatcgattc gattaaatga tcctcagaaa ttttgctttc tctagggtct
     9841 gggataatcg atgcgggtac atatttagga acaaaataag aacgctcccg caccttcgtg
     9901 tcaacagatg cgtttcgtgt agtatgcttg gactctgtcc ttgtttcact ttgtctgaca
     9961 gaacgagagg aatcaccaaa aagttttttt cgtcctgtcg atgtgatttc tgattggtaa
    10021 tggccggtat aatccggtaa ttttccttta tgcttttcca aattttcctt acgcggagct
    10081 gacttcaatc catttgttgc acgacgcttg cgacggattt tctcggtgtt cgtgttcgtg
    10141 aaatgaaatt ctttcatctg ttcaaacggg gactgattct gttcagtcaa aagatcttct
    10201 tgtccgtcaa ataatactcg cttcttgtcg ttggttaatt ttacgcctaa ctcatcatcg
    10261 tacgcaggaa aacggaaatg ttttcgtctg atttctttca ttcaaaccat ccttctcaaa
    10321 aatggaactt ttctcaataa tagcacagat tcattgtttt cagtagcaat gcgctgatta
    10381 ttttaagaaa atagcgatga atttcataaa tatatatata gtaataagac ttaattcgtt
    10441 gtctctctga taactagttc aggaaaaaaa cgacgataac cttgactcgc ccctttttct
    10501 tctatttttt ttaacaacat ttctccacat acttcaccca tacgtataat atcttgcttg
    10561 atcgttgtca atttaggtga actgatttgg tctaaaaata ccccatcaaa tccaatcaca
    10621 ccaaattctt cgggcaagtg tcctccagca tttataatcc ctcttactgc tcctagtgct
    10681 agccgatctg aactacatac aaaacaagtt ttatctgtaa aatctgccca gttttctgtc
    10741 atatatcttt ctacttctgt cgaatgattt ggaaaccgca agattttact aggaagatca
    10801 acttccttca tcacagataa ataaccagtt tcccttgaaa tttcaaaagg ctcgtccact
    10861 tcgataccga tataaataat cgtttgataa tctctttcta aggcatattg cgttgctttt
    10921 gctgtaccat attcattatc gctgtctaca taatcatatc cttcagaatt ttccccaaac
    10981 aagacaactg gtttttccaa acggtcgatc caatcgaagt ctttttttct gacacctgtg
    11041 atgatgtatc catcacaaga accgacgtcg aaggcatcat ttgtaactaa ttggagagaa
    11101 taatgcgctt taccgatccg tttagcgatt cccatcagca aattcatgta atagggctca
    11161 gttgtgtcga tttcttccag tataaataat ttgatgatct gcgaccgatt cgtgacaagt
    11221 gcttttgcag caatatttgg acggtaatcc agttcagcca ttgctgagaa aactaactct
    11281 ttcaattcgt cagtcacctg ttccggatga ttgatgactc gagagaccgt catttttgaa
    11341 acattggcac gttttgcaac atctgctaaa gtgggcatgc tctcttcctt tctaacatgt
    11401 tgtcattctt actatcaatt ataccaaatt ttcctaacag agtttattat agcccaaaat
    11461 aaaaaacgga aaatcctcta tcgtcatgag tgattttccg tttcaattat ttatactttt
    11521 ttatcaaatt ttttgctaaa aaagtggaag cttcttccat tagttgtatt tgtaccatat
    11581 gtcgctgtgt ttttgaggaa cgaaaaacaa tttcttttcc gtaagactta tgccgatttt
    11641 tttctacaaa tcgttccgtt tgtatataag ggattttttc gtcagcttgt ccatgccaaa
    11701 agaacagagg acgtccagct aacttttccg gctgcaatga caaatcatag ctcttaaccc
    11761 atgaaatcaa atcaaaatag tcttttggta cgacataatt atattccgat gcgcgcctta
    11821 cgatttcttt tccatattcc aaaggtgcag gggaacccat caaacacacg cccgctacta
    11881 tatgaggatg tttagccaac aaggcgcatg ttgtcattcc ccccatcgaa accccgccaa
    11941 ctgcaagctg atcatgtaga agacctcttt tttctaaatg cttgataagt gtgtcaaatt
    12001 caaacagatt tccgtagata ctatcccaaa atgtatagga tggaatagaa gaaaccggct
    12061 gtttgcgctc cccatgattc atcgcatctg gcaggatcac acgaatgccc tgctgcgcaa
    12121 tttttcttcc ttgcgtcaaa actaactcct tacttgtctg ccatccgtga taatagatga
    12181 caacgggaat acgttcctct cttaattctt tcagaacaac ttctaacact ggaatatgat
    12241 ggacagaacg tttcaatata attggattca attgttactc acctattttc attaaatttc
    12301 ctgatcgcca tagcaaccat gctccaacag ttgtctggaa caggttcagt gtaccaaata
    12361 atataaatgc aggactgaat ggctcgggaa ataagcacag agaacaaagt cccaccactc
    12421 ctaatgctag cgaaaggagt ggataaatag aaaaattcaa aaaactggca acgctaccga
    12481 aaatattacc aataaacagt atcaaaaaga aaaaaacgct taaaattaaa ccatctgtca
    12541 gcgacatctg aaaaacaatg ttctctacct caattggctg ttgaatcgaa tagacaccta
    12601 agtatatact gagaaggaga cttagcacgt ggctggcaca aaacacacga ttgaccgttt
    12661 tcattttaat cacctaacct atctttataa ttctattata atcattttat catttttttg
    12721 tatgtataaa gtttcaattt ttcttcactt acgatttttt ttgatattgt tgttatataa
    12781 atgaaaggag ttttacaaat gattgatcca aaaatgttgg ggatgatctt cctcattaat
    12841 tttgcttata tcactctgaa cacgttacgt ttcatgttga caatgaaagg ctatcgactg
    12901 attgctccac ttgtcagtat ggtcgagatc acgatttata tcgtcggctt atcccttgtt
    12961 ttagatcgtt tagataatcc actgaattta tttgtctatg cgttaggtta cgcagtaggt
    13021 atcagtgtcg gaattttgat tgaagacaaa ttggcattag gatacatcat ggtcacaacg
    13081 attttgccag caaatagcgc agaagataaa agtttgccgg gtatactgag agaacacggt
    13141 tatggtgtga cacagacttc tgcaatgggg ctggaaggtg agcggctagt tctcgaaata
    13201 ctttctccta gaaaaagcga acgtgaactg tatcgcctga tcaaagaaat cgaagagcgt
    13261 gcttttatca tttcttatga gccaaaatat atttcaggtg gtttctggac taaaaaagtg
    13321 agaaaacgac aaggaaaata actcacaaac ttaaaaactg tctaatatct aaattaaatt
    13381 ttaaatgtta gacagttttt atttattatt ttgattaaaa aacatcgaca tatactcatc
    13441 aatttgttat tatactttat atacttatca atttaatttg gagatattat ggaaattcaa
    13501 cgtagaacca ctaatttttt acatttaggc tctttttttg caactttgac gatcttattt
    13561 tttctatgtt attcatacct taagacctca catgcgcctt ctagtgcatt aggctggttg
    13621 cttttggatc attctgatat cacagaagcc gaagaaaatc cgtttttttt gattttgtct
    13681 tctctttgcg gcttactgtt ttctgtttat ttcggtgcct atttttttca tcgacatcct
    13741 ggtacgttaa acgaagcttc aagcgtattg attgcattta aaacgttttc ttttttagca
    13801 attctttttt attcgtttaa tcacaatacg cttttcttct ttatcatgaa tggtttagtc
    13861 gtgattttat ccatttttac ttattttatt ttttcactca tttttagcca attgaaatgt
    13921 ccgctcttct ttcgattagt tcctgtcacc tgttttattt acagcttgtt ttcctttttt
    13981 gtccttgctt taatgccact gaccaaccct tcgacgatcc aagtcgcagg aaatctgttg
    14041 gatatgctct tcatcgtcag tctaggtgta tttatgtggc aaagaaaaaa caaacaaaat
    14101 aaaaagcatt ttgaagcctg actattacga cttcataaat gcttttttag ttttaaacac
    14161 ttcaatctaa aagaataacc caagattgga taaattatta atgactcgcc attcgtttct
    14221 tagtgattct ggattatcaa atggacttcc taatctaatc agctagttct tcccataaaa
    14281 tgccatactt gactgaatca taatattgat tatttaaaaa tctaactttt cttatcacta
    14341 cttcctttgt cattccacat ttctcaccat ttttttgcat tcctttgttt ccagaccatg
    14401 tagtgaatct aataggtggt agataggcaa aatactcaaa taattctttt aaccataaag
    14461 gaagagaaag gctcctcctg caagagtcag tataattttt gcttactgct ctcttaggtt
    14521 ttactcacta aaactgttcc atattctttg atataagaaa atgcatgcgg tataaatttc
    14581 ttgctcagct cagaaaagat ttgtcgttta agaaaggcca tttcctccat tgtcaatgaa
    14641 ggatccaatg ttggtaattc aatctcaaaa ttgttttttt cgacaatagg taattctaat
    14701 gtgaatgatt cttcttcctt tatagtattc gtcacaagta gagactgatg gaaaatacta
    14761 ggccggcgat ttttatattt attcttacgt atcctgtcaa cgataacttc tatttgatca
    14821 aaaaagtacg attcatttac gtctccaata agttcggcta attctttgaa tgccttcgtt
    14881 acaggaagct gattcttctg tacaaaaaag taattagcca aagtaagcgg gaagtgttct
    14941 atagaggaaa gtgtgataaa acggtacttc tcatttcctg cagttgtcct cagcaattct
    15001 gtctctcggc tgtttcttac tccataaaaa tacgcttctt tccgttcatc ttcgaaacaa
    15061 tacgcccaaa taacttctcc catcgctaat ttttgttcat caacagagag tctcttcaat
    15121 tgttcagcta tggtttcagc agccgaagtg gcttgttgca agtaatcttt aggatcaaat
    15181 attggaaagt tcaaatgata tcttcgttct tccctcttga ttaatcctgc atcgatcaat
    15241 aaatcaagaa aatgggaaaa tttattttgc gaaaattctt ttttcaattc tcttagaatc
    15301 gtttcttgtt ctcgatgttc atatagataa tcggtcaatg aagaaaaaag cggatcatcg
    15361 gtcagttttt gataaaagtt ttccttattt tttgaataat agttcatata tgttctcttt
    15421 tctatccgtt ttattttact taaaaggctt ttacgtaaaa aggaggaaga acaagagtca
    15481 taattgactg ttattctcct ccatttcttc ggcctttagt atgttctatg tttcttttta
    15541 tatcaatcgc ttatccattc attttgaatg cttcatacta ccatttgatt agctaaatac
    15601 agccgtcaat tgaggtacga cttgtttttt acgtgaaacg acacctttaa gcaaagcacg
    15661 gttgttggtc aaagttgttt ggaaagcttc ttctactttt tcttttcctt taccagcaac
    15721 aagtaattct gaatcactgt ctaagatgtt tgtgacaact aatacgaaca gatcgtagcc
    15781 atttgctttc atttcatctt ccattgctgc ttctagctct gcttgtcgat ccatcacttc
    15841 attaaggtcc actgtattga cttgagcgat acgaacgttg gaactgccca ttgggaaact
    15901 ttttgcatcc aaatcaagaa gaaccgcagc tgatttatca cttagatttg ttcctgcttt
    15961 caacatttca agtccataag tgtttagatc gacttcagcg attgctgcaa gttcttttgc
    16021 agctgcttca tcttcaggcg tacatgttgg tgatttgaac aacaatgtat ctgaaatgat
    16081 agctgagagc atgattcctg cgatttcttt aggaattgtt acttggtttt ctttatatag
    16141 ttttaaaata atcgtacttg tacatccaac tggttctgca cgatagtaca atggattagc
    16201 tgtttcaaag tttgcaatac gatggtggtc tactacagct aaaatattta cttcgctgat
    16261 atctgaaata ctttgttgga attcattatg atccacaagc atgacatctg cattttctgc
    16321 tttagctgac tctactactc gtggtgcaga tagctgaaag tggtctaatg cgtattgtgt
    16381 ttcttcatta ggtgtaccta gagcaactgc ttctgtttct ttacctaatt cattttgcaa
    16441 gtgtgaaaac gcaatggctg ccccgattgc atctgtatct ggattttggt gaccaaaaac
    16501 taaaattttt gacatgtcat tcgctccttt gtgatttctc ctatttaata atagcaaaaa
    16561 aacggaaaga agcaatactt atcttctttc cgatgcgacc tatcgcgtta aatattttgt
    16621 atactcttct gtatgaagca atcgtttagc attttcgaca cgttcttttg taggtggttc
    16681 gatcccttct aatggatatt tcaaaccaag tgtttcccac ttgtattttc ccatcgtatg
    16741 ataaggaaga atctctactc tgtctacatt tgacaaggtt tgaataaagt tgtctagtcg
    16801 gatcaaatat tcatcataat cacttcgctc aggaactagt acatggcgaa tccaaaccgg
    16861 tttgttgatt tttgacaaat attgagccat ttctaaaata ttttcattgt cccaatgcgt
    16921 tagtttttta tgctcttcat tatcgatgtg tttgatgtca aataacagta aatctgtata
    16981 cttcatcaat tcttgaaaac gtgaaaagaa aggttcttca tacgtaaaag gttttccgca
    17041 tgtatccaat gtagtatgca tcttccgctc ttttgctttt ttaaatagtt cgatcaaaaa
    17101 atcgatctgc aatagaggtt ctccaccact aacagtaata ccacctttat ctccccagta
    17161 aggtctgaag cgttctgctt tatctaaaag ttcatctgct gtgtattctt tccctccgcc
    17221 aatattccaa gtatctggat tgtgacaaaa ttggcaacgc atgcggcatc cttgcataaa
    17281 cacgacaaag cgcagtccag gtccgtctac tgaaccgaaa gtttcgatag aatgaacata
    17341 gccgattgtt ttttcttcca tttaagttca catcttttct ttgatttcat tttaagtaca
    17401 agttagctgt cgaagcgaag ctttctgaac aaaatttttc gcaattgccg cttcgttatg
    17461 tgtaaaattt acagggacgg aacatacaag tcctgcccct gtaaatcgtt tctttcaaat
    17521 aagcatactg attattcgat tttgatgcga tggctattca acgtcctttc gacgctaagt
    17581 cgaaaaactt ttttcacgaa ctttttacat tctgtcgtgt gaagttctca tcaagacatc
    17641 taattgttgt tctcttgtca agtcacggaa tttaactgca taaccagata cccggatcgt
    17701 caagttcgga tatttcattg gattttccat tgcatcacgc aataaatcat tactgaatac
    17761 attgacattc aaatgataag ctccttttga gaagtatcca tctaagacat tacgcaagtt
    17821 ttcgattctt gtatcttgtt cttttccaag acctgaagga ttgattgttt gggtatttga
    17881 aattccatcc aatgcacagc cgtaattcat tttagctgtt gagttcaggc tagctaataa
    17941 cccactttgt tccgcattgt aacttggatt tgctcctggc gcaagtggtt ttccagcttc
    18001 acggccatca ggagtatttc ctgtattttt accgtaaaca acatttgaag taatcgtcaa
    18061 taatgaagtt gttggttttg agttgcggta agttttatga cgtttgattt gtcccatgaa
    18121 gtattccaag atccatgaaa ctaattcatc tgcacggtca tcatcatttc cgtatttagg
    18181 gaagtcgcca tccacttcaa aatcaactgc tattcctttt tcattacgga ttgcttttac
    18241 ttttgcatat ttgatcgctg acaagctgtc tgccgcatga gagatcccag caatacctgt
    18301 agcaaatgtg cgttgcaagt cagaatccat caaagccagc tgagctgctt cataagaata
    18361 tttatcatgc atataatgaa tgatgttcaa cgtattgaca tatagttctg ctaaccaatc
    18421 catcatattc atgaagttat gtttcacttc ttcaaagtct agtatttctg atgtgattgg
    18481 acgatattct ggtccaactt gggcgccagt cacttcatcg actccgccat tgatggcata
    18541 taatagtgtt ttagccaagt tagcacgagc gccaaagaat tgcatgtctt ttccgacaac
    18601 tgttcctgat acacagcaag caatcgcaca atcatcagaa ccccattgtt cacgaagctg
    18661 attatcattt tcgaattgaa tcgaagaact ttcgatagcg attctgctag catatgttct
    18721 gaatccttct ggaaggtgtt cagaatacaa aacagtcaag tttggttctg gtgaaggtcc
    18781 catatttgtt aatgtatgaa gcagacggaa atcattcttt gtaactaatg aacgaccatc
    18841 taatcccata cctgcaatgg ataaagtagc ccagataggg aatcctgaga ataattggtt
    18901 atattcaggt gtacgcgcga atttgaccat acgtaatttc attactaagt ggtcgatcaa
    18961 ttcttgtgct tctttttctg tgattactcc attatccaaa tcacgttgga tatagatatc
    19021 aaggaatgct gagatacgtc cgattgacat cgcagcacca ttttgtgatt tgattgctgc
    19081 taaatatccg aagtatagcc attggacagc ttcttttgca tttgcagctg gtttagaaat
    19141 atcatagcca tatttcgcag ccatttcttt catttgcgct aatgcacgat attgttcagt
    19201 gatttcttca cgtaaacgaa tcacttcttc actcattgta tgataaccag tcatatcatg
    19261 atcttttttc ttttgttcca tcaaatagtc gattccatac aatgccacac gacgatagtc
    19321 accgatgata cgtccgcgac cgtaagcgtc tggaagccct gtaatgattt tgtttttacg
    19381 agcagcacgc atttcagcag tataagcgtc aaatacacct tgattatgtg ttttgcgcca
    19441 gtcagtgaaa atatgtgtca attcatcgtt tggtgtatag ccattgcttt ttagcgcatt
    19501 gttagccata ttgattccgc cgaacggcat gaatgcttgt ttcaaaggtt tgtctgtctg
    19561 tagaccgacg attttttcca aatctttatc caaatagcct gcatcgtgtg aagtaatagt
    19621 tgatacgatt tctgtgtcca tatcatagac accgttacgc tcgaattgga tatcatttaa
    19681 cttttgcaat tcatcccata atttttctgt tgcttctgtt gggccttcca aaaagctgtc
    19741 atcaccgcga tattcagtgt agttattttg aataaagtct ctagtgtcta cgccttcttt
    19801 ccatttggta cctttaaatc cattccattg ctccattgta tggacctcct aaatttctgt
    19861 tttatgtaac agtttttacg tgattagtat aacacagcac cctatctttg caagggtttt
    19921 cttgtttttt atttaacaat ttttatgttt tatttacata tatttaaatc aacgttgaca
    19981 taccaacatt taagcattgt gaacactttc atcttttttt gcaaacgtat aaggatttat
    20041 taaaaatgaa tgaaaaagag acgccacctg tattaataca gatggcgtct cttcattaaa
    20101 atactacaac agttacgact cgatgaaaat ctcaggagca tcgataactt cgatgacttc
    20161 tccacctttt ttctcatcca tcgcaaatga tccattcgat gttcgatcac ctatagggaa
    20221 atcagttggg tcaatcgtta actgctgccc tttttgattc atcaaggtta tttcttttga
    20281 tcttccttcc gacatataga taatacggtg cggatttttt ttcagctcac gcatgaccgt
    20341 cactccccgc ttcgcacgtg aagtttggct gatttcttga gcaagcatgc gtttaacagc
    20401 tcctcgctga gtaacgatta cgatcggcgt atcaccttct gctaacacaa gcagcccgtt
    20461 gataatccta tctccatctt tcaaattcat agacttcact ccagctgcct ttgctccaac
    20521 aacgggaaca tctgttaaag gatagcgcaa acccattcct tgatgcgtta ctaagaaaac
    20581 atctaagcct ggctgatctt ctttcagata cacagcaatc agttcatctt cttccgcctt
    20641 tagcttcata cagctgagcg gacggctctt ataggtgcgc catggttcga actctgtcat
    20701 tcgtgtttgc ttgatcaatc cattcttgct gatgaataca aatgttttct ctgcttgaat
    20761 tttttgatat gggaaaacag ctaaaatcga ttcgtctaca gacagattgg agattgtttg
    20821 agaaatgtgc tcaccagcat ctttccattt caaatcgggt agttcatgga cagggcggta
    20881 gatcacattt gctttatttg tgatcagaag aacatggtcc aatgtactca attcacctga
    20941 gtatagcaag tagtcgcctt ctcgcatccc gatttcttct ggtttggaag cactataaga
    21001 acgaaggctt gttcgcttca catagccttc tcgcgtcaca ctgacaatta cgtcttcttg
    21061 tgccactaag acttgcgtat caattttgat ttcttcgatt tcgtcttcga ttgtcgttag
    21121 acgagcggaa gaataatttt ttttcacttc tcgcaattct tttttcataa cagagaaaag
    21181 ctctttgtca tttgaaagga ttttattcaa ctctgtgatt tgcgcaatca agctttcgga
    21241 ttccttttga agctctgtga tatccgtgtt cgtcaaacgg tataattgca atgtgacaat
    21301 tgcttctgct tgttcttctg taaattgaaa gacgtcgact aaattctttt tggcgtcttt
    21361 tttgtcttta ctttcacgaa tcgttgcgat tacttcatcc agaatcgata aagctttcat
    21421 caatccttct acgatatgct gtcttttttg agctttggct aaatcgaatt gactacgttt
    21481 tgttatgact tgctttcgat gctcgatata acttcttaaa atgtctttca gtccaacttg
    21541 atgcggagtc atatggtcga tagcgaccat attgaaattg taattgatct gtagctcagt
    21601 gtttttgaac aaataattca aaattccttg agcattggcg tcttttttca attcgacgac
    21661 aatttgcaga cctgttctat cactttcatc tcgtacttca gctattccat cgattttctt
    21721 attcaaacgg atctcatcca tttttttcac caaggtcgct ttatttactt catatgggat
    21781 ttctgtgatc acgatttgct gtttattacc cttcattgcc tctatctttg ttttcgaacg
    21841 aagaataact tttcctcgtc ctgtttcata agcttttttg atttcatctt ttccttgcaa
    21901 gatccctcct gttggaaagt caggtccagg aatatattcc atcaattttt caaggctcgc
    21961 ttgtggatga tcgatcatat agattgtgcc atcgatcact tcagctaagt tatgagtcgg
    22021 gatttctgtt gcatatccag cagatatacc tgtcgatcca ttgacgagaa ggtttgggta
    22081 tcgagctgga aggactgttg gttctttttc tgtatcatca aaattccaca cgagatcgac
    22141 tgtttctttc tcgatatctg ctagcatctc tccacttaac ttggataatc tggcttcagt
    22201 ataacgcatc gcagcaggtg gatcaccgtc catactcccg ttattcccgt gcatttcgat
    22261 cagtacttcc cgcaacttcc agtcttggct catgcggacc atcgcttcat aaatactgat
    22321 gtcaccatga ggatgataat tccccatgat atttccgaca gattttgctg atttacggaa
    22381 tcctttgtca tacgtattgc catctttgtt catggcaaat aatatccggc gttgcactgg
    22441 cttcaatcca tcacggatat caggcagtgc acgttcttgg ataatatatt tggaataacg
    22501 tccgaagcgg tcacccatca cttcttctaa agttaattct tgtacttctt gtcgattttc
    22561 catcaggtga cctcctattc tacttcaaat aactgattgt tttcattttt atctgctcgt
    22621 tcttcatcta ataaatcatt agaaactgaa ggagagattt cagtatcctc ttttttatct
    22681 aagatactgc catcttcttc gagcgtaaat tgcacgtgat tttcgatcca ttttcttcga
    22741 ggttctactt tgtcacccat cagtgtggtt actcgacgtt cagcttgcgc agcatcatcg
    22801 atacggacgc ggatcaacgt acgggaagtc ggatccatcg ttgtttccca caattgttcc
    22861 gcattcatct ctccaagtcc tttatatcgc tgcagcatat atcctttgcc gactttttta
    22921 atcattgctg ccaattcgtc atctgtccac gcatattcaa tgacttgctt ctttccttgt
    22981 cctttggata ctttatataa aggcggtaac gcgatatata ctttcccagc ttcaatcagc
    23041 ggtttcatat aacgataaaa gaaagtaagc agtaatactt gaatatgggc gccgtctgta
    23101 tccgcatcgg tcatgatgat tactttgtcg taattacagt cttcgataga aaactcagga
    23161 ccgactcctg caccaatcgt atagatcatc gtattgattt cttcattttt caagatatct
    23221 tgcattttag ctttttcggt attgatgact tttcctcgta aaggtagaat tgcttggaac
    23281 tttctgtctc ttccttgttt agcagatccg ccagcagaat ccccttcgac taaatacaat
    23341 tcatttttct tagggtttcg ggattgtgcg ggtgtcaatt ttcctgaaag aagagattcg
    23401 ccttttttac gcttcttccc atttcggctt tcttctctgg ctttacgtgc tgcttctctc
    23461 gcttctctgg ctttgattgc tttacggacc aacatctggc tcatttcgct attttcctgc
    23521 aaataaaagc ccatttgttc actgatgacg ttatctacta cggtacgagc aactggcgta
    23581 ccaagttttt cttttgtttg cccttcaaat tgcagaagat tttcaggtac acggatagaa
    23641 agtacagcag ctaacccttc tctgaaatca ctaccttcca gattcttgtc ccgttctttc
    23701 agcaaaccaa cttttctcgc atattcatta taagatttcg tcatcgccgc tttcatacca
    23761 gcttcatggg ttcccccatc ttttgtccga acgttattga caaaagataa aacattttct
    23821 gaataaccgt cattgtattg ataagcgacc tctacttcga taccttcttt ttctcctgaa
    23881 aaataaacaa ctggtgtcaa tgtatctttt tcttcattca gataatcaac aaattctttg
    23941 attccttctt cgtaatgaaa aatttccttg actggttctt ctccacgaag atcagaaaga
    24001 gagattttaa cgccttttag aagaaaggca gattctctta accgttcggc taaaatttca
    24061 tatgaaaatt tcgttgtact gaatattgta tcatctggta aaaaatggac agtcgtcccg
    24121 tttgcttttt ttgtttttcc tatctttttc aaagtaccgt caggtttacc gccgttttta
    24181 aatttctgct gatactctac gccatctcta acgatcgtta caattagcca tttcgataag
    24241 gcatttacca cactggctcc tacaccgtgc agtccaccag aagttttgta ccctccttga
    24301 ccaaactttc cgccagcatg cagcacagta aaaatcactt caactgttgg tattccggaa
    24361 gcatgcattc ctacaggcat cccacgtccg ctatctgcaa cagttatact attgtcttca
    24421 tggatggtca catcaatctc actgccataa cctgataatg cttcgtcaac tgcattatcg
    24481 acgatttcat agactaaatg atgcaagcct cggctgtcag tggatccaat gtacatccct
    24541 ggtctttttc taactgcctc taatccttcc aatacttgga tggacgagtc attgtattcg
    24601 ttgtttactt tttttgccaa ggcaaacgct ccttcaataa tactttgcgg caaaaccgca
    24661 ccacgattat catactagaa aaggtcgtga aaaaaaagag ggtagacgag aaatcaattt
    24721 taattatctc ctaaccgctt tttcgacatt cccctctatc gatcatctct tctttgtggt
    24781 catttttatt tttggttttc tttcaactta ccaagttcaa tcttaataca acgatccatt
    24841 acgatatctg tacgtcctgc ttttcttagc atctcagctg cttctttgtt ttcaatccct
    24901 aattgtgccc agaacacttt agcatcagtt ttcaaaaaat cacctgctat ttcaggtaaa
    24961 aattcactcc gtctaaatac atctacgata tcgattggat catcgatttc tgtcaaattc
    25021 gcatagactt tctcgcctag aatttcttca ccagccaata atggatttac tggtatgatt
    25081 ttatatccat tgtcttgcaa tacctgtgca accatgtaac ttgttcgttc tggtttattg
    25141 ctcaagccta cgacggcaat attttttgct tcttccaaat agttgaaaat cgtttcttgt
    25201 gttggatttt ttacagacat ttctgccacc ccttgctttt ctttttattc catctattat
    25261 acttgtatct ttcatttttt caattgttcc tgttacctat tcatgctaaa ataaaggata
    25321 gttttcaact tgaaagaggg taaaaaatga aattgatctt attacttatt ttagcatatc
    25381 ttttaggttc tattccttcc ggtgtctgga ttggcaaatt gtttttcaaa aaagatatcc
    25441 gacaacacgg gagcggtaat actggaacga ccaatacatt tcgtgtatta ggcaaaacag
    25501 ccgggattgt tgtcctgctt atggatattt taaaaggtac attggctact tgtttgccga
    25561 tgatcttcca cctttctggg attaaccctc tttggtttgg ggtatgcgcc attcttggcc
    25621 atacatttcc gatttttgct gggttcaaag gaggaaaagc tgttgcgaca agtgcaggaa
    25681 tgcttttagg atttaatcct gtattttttg tttattcttg tatcatcttt atcgttagtt
    25741 tattttgtac aagtatggtg agtttgagca gtatgatcag tgcagtattg atcactttat
    25801 ctacgatcgt ccttccttat gtagctcctg tgattttggc taagcccaat tggttactga
    25861 cgatcattgc ctttttagtt tcttccttta tcttttatcg gcataaagac aatatcaagc
    25921 gtatcagaaa ccatactgaa agtcgaatcc cgtttggttt gaatagcagc aagaaaaaat
    25981 cctgatactt tttcattcca aagagataaa acgggagtgt gacaaatcga tgtcacactc
    26041 ccttattact tacaggatag cttttttaca caccttggta aacggtgttc cctgctaact
    26101 tctcggtcac aacctcttac ttcactgtga tggaatattt tgtcttgaag atttcatggg
    26161 cacctagttg attgatccct actttttcca tcaagtttcc actactgttc accgtgtcag
    26221 cgattccgca ccacggttcg atacaaacga aaggcgcttc ttttggataa ggagaccaga
    26281 tccctacata aggcatatct ttatagctta aagttacgct atgggaagat ttttcacttc
    26341 gaatggcgta cgcatttaga ccccttgttt caaaaaccaa ggcatcttgc tcaaaaagtt
    26401 cacgattcaa agccagattc gtatttgtct gacctaacgt cttttgatcc atatcgatga
    26461 atgggccttt caatggtagg cgtaatcttg atttcaatgg agaaaatgcc agatagaaat
    26521 cttcaaatga caatccttct tcaagaggta cattgaaagc aggatgtcca ccgatagaaa
    26581 agaacatttc tttttcaccg gtatttgcca cttgataatg aaccgtgatg ccgtctcctc
    26641 ctaattcata gctgatcact aattcaaaat caaatggata cttttctaat gtctccggcg
    26701 tactccttaa gatgaaacta gccatttctt gtccatgttc aatgacttca aaatccatat
    26761 cccgagcaaa tccgtgttgc cccatttgat atgttttccc ttgataaaca tactgatcat
    26821 cttttaaacg tccaacaaaa gggaataaaa ccggtgcatg tctgccccaa tacgctggat
    26881 caccttgcca gatatactct aaaccatgtt cttttgattt cagactgatc aattccgcac
    26941 cgtgttcagc gatttcggcg atcagctggt cattttgaat aataacagtc attgccattc
    27001 ctccaacttc gacttacttt ttaaaatggg aaggctggaa caaacatttc tatctgcccc
    27061 agtcccctta cttctaataa ctgtgttttg ctaactcgtc tttaaaacgg gaattcaata
    27121 cttttaaata agttcccttc atgcctaacg aacgagattc aatgattcca gctgattcca
    27181 attttcgtaa agcattgacg atcacagaac gagtgatgcc gatactatcg gcaatattag
    27241 aagcagtcag acgaccttca tctccatcca atgcttcaaa gatagcttgt acagccttca
    27301 attcgctata agataatgtg ttgattgcca tttggacagc cgttgcactc cgtacatctg
    27361 cttcgatgct tcgagacttc tgataaagga tctgcatacc gacaaccgta gctccatatt
    27421 ctgctaagac tagatcgtca tctgcaaacg attgttccat acgagataag ataatcgtac
    27481 ccaaacgttc gccagctcca aagatcggaa cgacagtcgt cagaccaaat ggatatttct
    27541 ctcttgactc taccggaaat gcagtaatat cactagtaat tgggatatta gcttcagttt
    27601 tcatcaactg tgccactgta tcagtataag attcaggaaa ttgtttctca acaaacattg
    27661 ttttgattcg ttcattgttc acatcgtgac gttcattata acctagtaat actccgtcac
    27721 ttcctatgat ataggtgttg ctgtcaagaa taccacctag tgtgacagcc attttatcgt
    27781 aaggcaaatc tgcttgcatg tcaaaagtat tcttttgctg caaaagtgtg tttatcttgc
    27841 gggttttcgt tagcaagtct gtcattgtcg tcctccgatt ttttataaaa tatagcgact
    27901 taaatcttca tttgccgcaa ttgagccaag tttgtcatcc acatatgctt ctgtgatcgt
    27961 tatttctccc atttgcatat ctggtgcttc aaataataga tcttctagta gtttttccaa
    28021 aatggtatgc agtctgcgtg cgccgatatt atctgtgtca cggttgacat cgaaagcgat
    28081 ttgagcgatc cgttcaattg cttcttttgt aaacgtaact gacacatttt ctgtaccgat
    28141 caacgcgacg tattgtttta ctaaagcatt atttggttcg gtcagaatct tcacaaaatc
    28201 ttcagctgtc aaatcatcta attctacccg tatcgggaag cgtccttgaa gttctggaat
    28261 tagatcactt ggtttagaca aatggaaagc tcctgaagca atgaatagga cgtgatctgt
    28321 ttggatgatc ccgtattttg tgtttacttg tgaaccttcg acaattggca atatgtctct
    28381 ttggacgcct tctctggata cttctccaga ttgctgtgat ttagaagtga tcttatcgat
    28441 ttcatcgata aaaataattc cactttcttc cgctaatttg attgcctcac tatggatatc
    28501 agcatccttg actaattttg ctgattcttc cttgatcaat aactctaaag cttctttgac
    28561 tgttaccgtc cgttccactt ttttcttcgg tgtcaaagca cctagagttt catttaggtc
    28621 gatccccatt tgctccaatc cattgttcat cattggtgca ggtttctttt ggtcttcaac
    28681 ttcaatggtt acttcacgac tatccagtaa acccttttgt agttgttcca agactgtacg
    28741 gcgattgatt cgaatatctt ctgtcacttc ttcttgcggt tcagcggtat tttgctgact
    28801 agcattaaaa agattcatca tttgttcaaa ttgattagtg gattgtttct gttctttttt
    28861 gataccaggt accaagattt ttaccaaacg tttttctgca ttttttttag cacttgtgta
    28921 tacacggctg tactgctctt tttctacgat ttggatcgca ttttctacta aatcccgaac
    28981 cattgattct acatctcttc cgacataacc gacttcagta aattttgttg cttcgacttt
    29041 gacaaatggt gcattgacga tttttgccaa acgtcgtgcg atttctgttt taccgacccc
    29101 tgtaggtccg atcatcagca tattttttgg tgtaatatct tgttgcattt tttcatctaa
    29161 ctgcaaacgg cgatagcggt tacgcaaagc aacggcaaca gatttttttg ctgcgtgctg
    29221 tcctacgata tattcatcta attcacttac gatttgtctt ggtgtttgtt ttaattcata
    29281 catatctact cacctcttac agttcttcta cgataatatt atgattagta aagatacaga
    29341 tatctgcggc aatatttaac gcattttctg caatttcttt cgcactcatg tcgttttgag
    29401 catagttttt cattgctcgg gctgcagcca acgcaaaatt ccctcctgat ccgatagcta
    29461 agataccatc atctggtgtg ataacttctc ctgtacctga aacaagaagc atttctttat
    29521 cattcattac gatcagcatc gcttcaagtt tttgcataga ttgctgtgtc cgccattctt
    29581 gagctaattc gacagctgcg cgttgtagat tcccgttata ttcgtttagt ttgccttcaa
    29641 atttttcttc taaagtaaac gcatctgcaa cacttccggc gaaacctaca acaacttctc
    29701 cattgtagat acgtcgtact ttctttgcag tgcctttcat gacaacagat tcacccattg
    29761 ttacttggcc atctccagcc atagcaaatt tcccgtcttt ttcgacggca cagatagtgg
    29821 ttgaatgaaa tggtgattcg accatgtttg tttcctccta aatttgttct tttccttatt
    29881 tttacgctct tggatggaaa gaacgatagt ttttttgtaa actttctttt gttacatggg
    29941 catagatctg ggtagtcgat aaattggcat gtcctagcaa ttcttggact gtccgcaaat
    30001 ctgccccatt atttaataaa tgagtagcaa atgtatggcg cagcatgtga ggatggatat
    30061 cactatccaa actgcttttt ttgatcattt gattcaagac atattcaatt ccagtaggcg
    30121 tgattggttt tccatgatga ttcacaaaaa cgatatcatg ctcttcgtga tattttgtca
    30181 tcaatacctg gcggccttcc ctgaaatatt cttgcagagc atcttgagca aaagaaccta
    30241 gaggagcata tctctctttg ttccctttac catggatcaa catgacctct gaatcaaaat
    30301 caatgtctga taccgacaaa ctgctacact cacttaaacg gatgcctgac ccgtaaagga
    30361 cttccagtaa agcgcggttc ctcaaatcca aaggactatc tcctgctacg ctgtcaaaaa
    30421 gtgcttgcat ttcattttca taaaaaaaac gtggcagtct caaatttttc tttttgagat
    30481 gaacgtaaga aaatggattt tcttttatca cttcttcttt gagaagatac tgataaaacg
    30541 aacgtaggct ggctatcttg cgactgatag aatttctgct atagctagga cgatgatcag
    30601 ctaatgcact caaatagaca cgaaccgtaa aatattccgc cttcagcaga tcagcgtctc
    30661 cagattcctc caaaaaatgt ttgaaatgga gaatgtcttc ttcatatgct tctttcgttt
    30721 tttctgaata tcctctttcg acaatcaaat aacgcaaaaa tttttgttcc cacgtggctt
    30781 ctgtcatttt ctcaccttcc ttttgtccca gagaataatt tagcacagcg tttataaaaa
    30841 gacaaataaa ttgtcagaat ttaaccaata tttctaagtt tgttcacaat tggcgcaatt
    30901 cctcacattt tgtttgagaa attcttaaga aaaacgcaag aaaaagagcg agactatccg
    30961 tctcaccctc ttcatggagt ttccttactc atttgggatt gcagtttttt ctaattctgt
    31021 ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt gcttcatatc ttgctttttt
    31081 gtctcgaatg cgctctggca gttcaggaaa cagaccatag ttggcattca tcggttggaa
    31141 atgttttcct tccgcatgag taatgtagta agccatactt ccaatagctg tttcttgtgg
    31201 aaactcgact ggttccattt cttttgctaa gcgtgcggcg ttgattccag caagtagtcc
    31261 gctcgctgca ctttcaacat agccttctac accagtcatc tgacctgcaa agaacagatc
    31321 ttcgcgtttc gttgattggt aagtcggttt cagcaattct ggcgaattca taaagctgtt
    31381 tcggtgcatc acaccatagc gaacgaattc agcattttct aacccaggaa tcatccggaa
    31441 aacacgtttt tgctcgcccc atttcaagtg agtctgaaag cccacgatgt tgtataaaga
    31501 agctgcagca ttgtcttgac gcagctggat aacagcataa ggacgtttgc cagttttagg
    31561 atcttccaat ccaactggtt tcataggtcc gaaaagcatc gttttgaatc ctcgctgtgc
    31621 cattacttca attggcatac atccttcaaa atatttttct ttctcaaaag atttaagttc
    31681 tgccacttcg gcattgatta gtgcctcatg gaaagctttg aattcctctt ctgtcatcgg
    31741 acagttcaag taagctgctt ctcctttgtc atagcgggac ttcaaatata ctttatccat
    31801 atcgatcgta gctttgtcta cgattggtgc agctgcatca tagaaataaa aaccatctga
    31861 accattgaat gctttgatgc tttctgctaa tggatgagaa gtcaaaggtc ctgttgctac
    31921 tacggtgatt ccttccggta tttcagtaat ttcttcgttt atcactgtta ctaatggatg
    31981 ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg cggtcaacag ctaaggcacc
    32041 accagctggc actgctgttt catctgccga agtgattact accgaattca atcggcgcat
    32101 ttcttctttc aataacccta ctgcattagc caaactgttt cctcttaatg aattggaaca
    32161 tactagttca gcaaaattac ttgtgtgatg cgcctcagtc gatttgactg gacgcatttc
    32221 atataaacgg acagggactc ctgcttgtgc gatctgccaa gcagcttcac ttccggcaag
    32281 ccctgctcca acaacattta ccattttcaa tgaaagaacc tcttttctat tatttttgga
    32341 tgtcttcttc gtaatctcca tttacacaga ctacttgttt accgcctttg actttcttct
    32401 cgaccagata tccgtcacat tttggacaat ttctgcccac tggtttatcc caagaagtga
    32461 attcacattc tgggaaacgg gaacaaccat agaatagtct gtttttctta gattttcgtt
    32521 caatgacttg tccttcatga catactgggc aagtcacacc gatttctttt acgattggtt
    32581 tcgtattccg acaatcagga aaattactgc aagcataaaa cttcccatat ttccctaatt
    32641 ttatcaccat cggatgtcca cagacatcac aatcgaatcc tgctggttca tctttgattt
    32701 gaattttttc gattttttct tccgcattcg tcagttcttt ttcaaacggt ttatagaaac
    32761 gatcaaccac tttgacccac gcttccgtgc cttcttcaat tttatccaaa tctccttcca
    32821 tttctgctgt gaaatggata tcgacgattt gagggaagaa ttcacagatt aatgaattga
    32881 caatttctcc tagttcagtc ggctcaaatc ttttttgtgt caattttaca tagtagcgtc
    32941 tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg ccctaccccg ttttcttcta
    33001 gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg ttgagtaaaa tgttgttttg
    33061 gttcaatatc aacagattgg actacatcgc cttcttcaag ttctggaaga atattttctt
    33121 tctcttcttt gccgtcatct ctaccttcca catacacttg catgaatcct ttgaatttga
    33181 tttttgaacc gttcgcaatg aagatgacac cattttgttc taatgtcact ttcattgtat
    33241 ctaaaatagc tggtgtcatc tgactagcca caaaacgtga ccagatcaat gtataaagtt
    33301 tcaattgatc tttatctaaa tattgtttga gttcatctgg tgtacgaagc acactcgttg
    33361 gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt tttgatcttt cggtgatcat
    33421 gtctagaaaa ttcttttcca tacgtatctt caataaatgc tgcggcctct gcttttgctg
    33481 tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat caagccgact gttccttgtt
    33541 tacccaaagt gattccttca tataattgct gtgctaccat catggttttt cttgttcgaa
    33601 agtttaactt acgggcagct tcttgttgca ggctacttgt agtgaaagga ttcgcagggt
    33661 ttcttttgcg ctctttcttt tctacttttt taacttcgaa gtctgttcca tcaattctag
    33721 cagtgacttc ttttacactt tgtgcatctg gtaatttctt tttcttaccg tcgattcccc
    33781 agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt accgtcgatg ctccaatatt
    33841 cttctggaac aaattctcgg atctcttttt cacggtcaat aataattttt aaagcgacag
    33901 attggacacg tcctgcactt aaccctttct tgaccttacg ccagagaata ggactgatcg
    33961 aataaccaac gattctgtct aaggtacgac gagcttgctg tgcatctact aaatctacat
    34021 cgatcgttct tggttcctta aaagctgctt tgactgcgtc ttttgtgatt tcattgaaga
    34081 ccacccgatt tttttctttc agatccaacc caagtaggta cgctaaatgc caagcaatcg
    34141 cttctccttc tctatccggg tcacttgcca agtaaacttt ttgagctttt tttgctgcgg
    34201 ctttcaggct tttgatgaca tcgccttttc cgcgtataga aatataatgg ggttcataat
    34261 tgttttcaaa atcgataccc attttacttt tcggtaaatc tcttatatgg ccaacactgg
    34321 ccattacttt atagtttcgt ccaagatatt tctcaattgt cttggctttt gcaggtgatt
    34381 ctacaatcac taaatattta tacgccatta ctgaatggac tcctttccat tgtcgtgttc
    34441 ctactattaa aagaggattc atacaaatcg tagctaatca tatccattca gaaaacgttt
    34501 gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa attctgccaa gatatcttca
    34561 cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat gacatccggt agaatgttct
    34621 gttaaaatat ttcctggaac agaaaaaact gttcttccaa gatccaatgc ttgctgtgct
    34681 gttatcagag agccgctttt tttcttggct tcaaagacac atgttccttt agacaaagct
    34741 gcgataatcc gatttctcat tggaaaacaa tgctttttcg ggccggattg aggaggatat
    34801 tcactcaaaa gaagatgatc atttgcaatc acctcttgca agtcacgatt ttcagcagga
    34861 tagacttgct ctatccctgt tccaatgact gcgattgtcc gtccaccatg agagattgct
    34921 gcttggtgac cgtatgtatc tatccctttc gcgcagccac tgacgatcac atatccagct
    34981 tctatcactg gttttagtaa ataataaact gtttttttgg ctaaagcaga agtgtgtctt
    35041 ccaccaacaa cgcttaacat gttacaagaa agtaaagata gatcaccatt ataaaacagt
    35101 acaattggcg gataggtcaa gtgaagcagc tgagggggat atttgggatc aaagcaagtg
    35161 ataaacgact gtttcgattg gattttgccc cagttagcgt gaatttcctg ccaacttttg
    35221 cgaaacagct cccggtgttt ggttatttta cctatcttaa tgatgtcttc aaatccgatt
    35281 tctttataag gatactgcat catccagtta gcaagtgccc attttccttg actactgatt
    35341 ccttttgtat aagcaagttt caataaattt tcttctattt gatacatcat aaaaccgcct
    35401 ttcgtctctc tttctaataa gatacgtaaa agcggctgtt ttttattgta aatatttttt
    35461 tacaggtgca aaacttttac gatggattgg cgtaattcct tgtcgttcaa gtccttctaa
    35521 gtgttccttt gttccataac ctgcattatg cgagaaacca tatccaggat aaagcacgtc
    35581 atattctttc atcaattggt ctcgatatac tttagcaatg atacttgcag cagcaatcga
    35641 cacagatcgt gcatcccctt tgattatttt ttcctgctga ataggcaaat ccaaggtcat
    35701 cgcatcgatc aacaaatagc ttggtacaat ggacaatttt tctactgctt ggatcatcgc
    35761 taatttactt gcttcataaa tattcacatc atcgattgtc tgctgatcaa cgatcccgat
    35821 cccgattgcc agggcttttt cttgaacttg ttgataaaga agttctcgct ttttctctga
    35881 taattgtttg gagtcattca gccctaatat gatctcattt ttcggcaaga taactgcagc
    35941 tgccacgaca ggtccagcta atggtcctct tcctacttca tcaatgccgg caatccattg
    36001 gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt tcttcatatt tttccttaat
    36061 taatgcttcc tttttcaatc gtcgttcaaa agaagccagt gcttgctgca cgcctttgcg
    36121 tggatcattt cttaatgatt ctagtgtagg atcatctatt ctctgtatgc ttttcaatcg
    36181 ttctttgata ctttggatgg attcagtcat cacttttttg ctccatttct tcgcaacgat
    36241 ccagtgtgta tcgtccaagt tttccttgac gaatatcatg cacgactaat tcgctgccac
    36301 gatcatagtc ttctttatac ccacgttttg ctgtaatatc catcagtaat tccggtgcca
    36361 atttttcttc ttgctcgatt gataaaccat aacgatcgct gattcttcct gaataatatc
    36421 gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa atggacaagc tggtctttga
    36481 tcgcgcctgt cagcgctagt tttttgccga tttcttcatc ttcaaatttg ggccataaaa
    36541 tccctggtgt gtctaacaat tccaactcat tacctaaacg aagccactgc tgaccttttg
    36601 ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc tactagtcga ttcatcaacg
    36661 tagatttccc aacatttgga atacctaaga ccattgcccg aatagcgcga ggcttgatcc
    36721 cccgagcagc atctcttgcc cgtttttctt tcaatgcttc tttagccgca ggtactagac
    36781 ctttaattcc tttactttct tgtgccgtga tagctaatgc aagatggcct tgtttacgaa
    36841 aatattcttg ccacagcttt gtctgttgtg gatctgccag atccgcttta ttcaataaaa
    36901 caaggcgagg tttttgctga atgatctgat ctagcattgg attacgagaa gataaaggta
    36961 atcgtgcgtc taccaattca aatacgatgt ccacatattt cattttttca gaaacttctc
    37021 gtcgggcttt tgccatatgt cccggaaacc attggatggt cattgttctc tccattcctt
    37081 tcataccgga atattccccg gctttacttg tttttttctt tttcaatata aattcacttt
    37141 tttattataa agaaattcca tctattagtt aagtgaaagt gaaccagtcc tcattctatc
    37201 acatcttcac ttttttgatg atgcaaattt ttattttggt ttctatatat gaatctaaat
    37261 ttttgacagc gtttcctaaa taagagatga tagacgaaca aaacagaaag gagacgaaac
    37321 aaatgaatag tatggttttt gttaattttc ctgttgctga tgtcaatcgt tccgttgctt
    37381 tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc tactgaagaa gctggttcaa
    37441 tggtatggga tgacactttt tggataatgc ttctctctca tgatttttat aaaaaattta
    37501 taaaagataa agaaatagct gataataaaa aaacaagtgg tgctttgatt tcgtttagtg
    37561 ttgaaaatgc ggatgaagta aaaaaatttg ctcaaattgc aaaagaaaat ggtggtactt
    37621 tccacgaagt agatatggga atgcctgaag aagaaatgtt ttcattagaa gtccaagatc
    37681 tagacggaaa tacattagaa cccgtatgga tgaagatgta agcagataac taatctgacg
    37741 ttttttataa caaaaaaaca aaggcggatg atacgataag tattctccgc ctctattttt
    37801 tttattcgta caatctcttt tttacagttt ataaaagcag tcttctcacc ctttttttaa
    37861 ttgttagatg ccagtgtatg taaatcacca atgtgtcatg acacattggt gattttatgg
    37921 tatcctttta ttattaactg acaaaggaga gaatattttg aacactttga ttgcaaaaga
    37981 cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg acctctgctc ttttattagc
    38041 ccttgatgtc atcttataca agatagcgat tggtcctgct tattttcaaa tcacattcgg
    38101 atttttaagt atggcgctta tgggttatct atatggaccg atctgggctg gtttgcttga
    38161 aatgttgtcg aacactttaa atttcacgat atttggttca ggaacgtttc aagctgcttt
    38221 tcttatcacg gctttccttg gtggatttat taatggctta tttttatatc ataaaaagat
    38281 aaacctgcct tccgtcatcg ctgcccaatt attgattatg atcccagtca gcctttttat
    38341 caattcttgg ctgatgtctg ttttgtttgg tgcagatttc aaagtaattt ttctaggtag
    38401 attcatcagg aatatgatcc aaatccctat tcaaataatg gttgtttatt catttatcaa
    38461 aactttagag gctagaaaag tgtttaaacg agctgtttga ttttacgtac taaggtagta
    38521 aactgatttt ggattaaaca ataaagcgtg cagtctcaac agactgcacg ctttattgtt
    38581 tctcactgga tccattttat ttcatcaagt ggataataga caaatcttgc tttacctaga
    38641 atatcagttt tattgatcgt gccaaacgaa cgactatctt ttgaaactct tcgattatct
    38701 ccaagtacaa aataattatc ttccggaagc ttggattgtc ccgttagctc ttgtagtgta
    38761 aagtccgtcg tataggatgc atttgcatga tctgatttca ggtttttagt tagataaggt
    38821 tccttaacta ctttcccatt gatcttcaat tgatcatttt ggtagctgac attttctcct
    38881 ggaagaccga ttactcgttt gatataaatc gtgccatctg ctaattgaaa aacaacaata
    38941 tcaaatcgtc tgatctccga aaatttttcc atcacaacca tatcaccttg cttcaacacg
    39001 ttttccattg aattgccttc cactggtacc gggatgaata aaaatccccg caacatgaac
    39061 gcaaggaata cagaaacaag taagtatttg aatacaagcc agaagcgatt aatatagcgt
    39121 tgtttttttg tcaatctcta tcaatccatc cctctttttt tcattctttc tttattataa
    39181 gaaaaatatc aaaaaaatgc gactagatag ctgaaataaa aataattctt tcaattaggc
    39241 tattggacaa aaaacttttt tgatcataaa gtacaaaact atctcttata tgtcatgatt
    39301 attttctaaa tatttttccc atgcttcgtc aaagatatcc atggacggca aatagtcgac
    39361 attcagttcc agataattag aaatttcttc gtagctagag ctgtgcttag gaaattggat
    39421 gtctttaccg gcttctgtag cgaaaacaga taaagcatca tgtgtgccac cgcctcgcag
    39481 tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt tcctccaatt ttcttcgata
    39541 aaggcagccc ggcgtgcatg gtttctttta aaattttcag gatgcttttt ataatactct
    39601 tgatggtctt cttctgctag ataaaatggt tgggctggtt cgatttttgt aacgattggc
    39661 tgttcgaaac gtccactttc agctaactgt ttcttacttt tttcagcaat gatccgctgt
    39721 tcttctgtca aataaaagat caccgggcga taattgtcac cgcgatcctc gaattgtccg
    39781 aaagcgtcag ttggatcagt ttgctgccag taaagttcta ctaattgttc atatgaaatc
    39841 aatgttggat catagataat ctccactgct tctgtatgtc cagtcgtttg gctttttact
    39901 tgttcataag tcggatctgg tacatgccct ccagtatatc ctgacatcac tgtatggata
    39961 ccaggaagcg tgtcgaaagg ctggatcata caccagaagc agcctcctgc aaatattgct
    40021 cgttccatta tgattccccc tctaatacta ctcatccgat gattttttat tttcttttgt
    40081 cttgctttca gtactgctgg tgctagtttt gttggtattt gagttgtttg aagcatcaga
    40141 ggtatctttt ggcaaatata gactaaaccg aatgtcatca tttactaagt cgattttatc
    40201 tgctttaata tacatcccgt tttgcattcg gaattgatcc aacctaagca tgatagtcag
    40261 atcatctgta ttgacttcga tccaatctgg taatttatag tttctcttga tcatattcat
    40321 cacatctttg attggcaaac tcaatgttcc gatagaaaga cttctagctt ttagttggac
    40381 atttccgttt tccatcacat acggatcaaa atataaatag aaatcgatag gaaaacctag
    40441 tacttcgaaa gtaccattga gtagtgcttc attttccaaa taaaatttat attcaatatc
    40501 actatctttt tgatactcac taaggaaaaa gctgatgatt tgattgactt tttctttatt
    40561 tgaattgatc gttaagacag gatctccttg tctttccgta agagctggta tttctgggag
    40621 ttctggttcc cgattagcaa aaatacggct gcctaaaaat acggtcgaac caagtactac
    40681 cccaagaagg ataaggaagg cgaatttcca gtaatttgtt ttctttgttt tgtttgaaga
    40741 tacgtcagat cttgttttca tgtttattga tcatcctcct tctctaacca ttggttttgt
    40801 gtctcgatca gctgatctct cactgcattt gccatcaatt gataacctaa attatttgga
    40861 tggaaatgat cttcttcata gagcacatta tttttgatgc catttagatt ttctgtcgtg
    40921 ctagttgttt gatctgttcc ttcttctgtg acacctactt cctgattcaa tccttgataa
    40981 agcaaatcat tgattgggat aaagtagcag ttcggcattt tttttgtcac cgtttctgtt
    41041 cctttgttcc aattgttaac aattgtctgc atgtcagtga tctcaggaaa gttcaaataa
    41101 aatggattgt agatgcctaa aatgtaaata ggtgcggttt tattgaggga tcgaaattct
    41161 tccaatagtt ctgtgacatt ttcctgatag tttttcagtg gctttttaaa ggaattgacc
    41221 gttaacccga ataaattatt ttggataact ttcatcaaat cattaccgcc aacagtcagt
    41281 gtgataaaat cagctgtttt gatattgttt cgaatttctt cttttgtttt tactcgcttt
    41341 aagatctggt cacttcgttc accatttaca ccatagtttt ctgtttcaac cgcggtcaaa
    41401 ttataacggt cttttagatc atttgcgaca aggggaacga atcctccttg cttggtttga
    41461 tctcctactc cttcagtcag cgaatctccg atagctatgt agtgaagttt ttctttttta
    41521 tttggatctt ctctcacagt agttactggt ttcattagtg ggccagcttt cggtatagaa
    41581 gcagctaaac ctgcatagat gatgatcgca attaataatg ggaacaaaac gaggatcaaa
    41641 tgtcgtttcg ttaatttcat tttttcacct cttgtattcg attggttcta atgttatcaa
    41701 aaattttatc aaaaaggtag tctttatgaa aaaagaaaac aattattttc ctaattttat
    41761 aaagaaaaag agaataagct gcaagccttt caggctgagg gcttatcctc ttttcgacta
    41821 atctacataa tacatgattg caaacgcgtc tttaccagta tgcgtagcga taaccggatt
    41881 cgtatgcagt acagggatat gcatgtccgg gaataattct tgcaaaccct ctttgaattc
    41941 ttgcgccaat tcttttgcat ccgcatgtga aataccgatt tgtcgcacat ttggcaattc
    42001 tttcagttca tttttcaaat tattaaacca tttatcgaac gttttctttc ctcgcccctt
    42061 agttacaggg attaattcag aatgggcgaa attcatcacc acgcgtatgt tcaaaacact
    42121 agacagcaaa ccagttgttc ggctgattct tccacctttt accaagttat ctagtgtgga
    42181 tattccgatg tataattttg tattgtcttg tacttttttg acttcttcaa ggattttctc
    42241 tactgtttta ccttcttttg ctaattcagc tgctcgaatt acctgaaaag ataatccttg
    42301 gtcagtggta tcactgtcga taaccgttac tttcgttttt gttagattac tggcttgtct
    42361 tgctgcttct actgttccac tcaagccttt tgtcatatgg atcgaaatca cttcactgcc
    42421 gtcttctcct agctcatcat acatcgagat gaattctcca attggtggtt ggcttgtttt
    42481 gggcagattt tttgctttcg ccatcatttc cataaatctt tcgccgtcta gttggtcatc
    42541 atcgggatat actgttccgt cgatcatgat ggatagcggc atgacacgaa tgcctaaagc
    42601 atcgcgtaca tttttttcca tcgtacatga agaatctgtt acgattttta catttgtcat
    42661 tattttctct ctctctttct ttaacaagat gccttattca tgttaaacta catgtaagac
    42721 aatttctatt tttagtataa cagacaaata atgatttttc attaattaca cctagtaaag
    42781 gagccgtttt catggaaaaa acgaaattta atcgaacgta tgaaatatta aatgaagtgt
    42841 ttaatgcagt cacccatgga attgggactg gattgagtat cgcgggacta gtggttcttt
    42901 tagtaaaagg tgcccacttg cattcctccc tccatattgt ctcttatgct atctatggat
    42961 cgatgatgat tcttttattt ttaacgtcta ctttgtttca ttcattgatt tttacaaaag
    43021 caaaaaaggt gttccaagta ttcgatcata gttcgatttt tcttttgatt gcaggaagtt
    43081 atactccttt ttgtttgctg agtatccgag gatggctagg ttggacttta tttgtattaa
    43141 tctggttgtt tgcgattctt ggcattatat ataaagcgtt gacgttgcat aaacgggaga
    43201 gagtatcaaa agtatctacg atcatctata tttttatggg gtggctctgt gtcgtggctg
    43261 ctgttcctct ttatcactct cttggaatga ctggtgttat tttgatggta gcaggcgggc
    43321 tttcttacac gataggcgca ttcttttata gcttgaattc tatccgctat atgcatgttg
    43381 tttggcattt gtttgttatg ttaggtgcag gttttatgta cttctctgtt ttattcttta
    43441 cttaataaaa aaaataaagc tatggatggg acagctctaa ataacagcta gtctatccat
    43501 agctttttta tatcgtcaat tcgcctgtcc cgctttttac aggattctca ttacgctgat
    43561 acgtttcaaa ttgatgcgga taacgatttt tttcatccat ttctccttga ctagtattga
    43621 tcagtgacca atcagaccag tcaacaggtg gaaaataagc atctccatcg aatacatgat
    43681 ggatgactgt acggtataat atcgtacaaa atggcagaaa ttcttgataa acagctgacc
    43741 ctccagcgat gaaaaagata ccgtctgttt ctttttccag agcaagcacg tcttccacac
    43801 tatgcatgac atgcacgcct tctgctttat aggaagaatc tctcgtcaaa acaatcgtct
    43861 gacgattcgg caacggacgg ctacccatcc cttcgaatgt ttttcgtccc atcactagtg
    43921 tatttgcttc tgtcatttgt ttgaagaatt tcagatcatt tggcaaacgc caaggaagac
    43981 ggtcttcttt tccaatcaat ccgttttcat cctgtgccca aattgcagct aacattttct
    44041 catcctttct gattctaaac agcgatagga gctttgatcg ccggatgtgg gtcatatcct
    44101 tctaattgga tatcttccat ctcaaaatca aatactgact gcttttcctg attcaaaact
    44161 aaagtcggaa atgcgcgtat ttctcggctc aattgttctt tcatttgttc gatatgattg
    44221 atataaagat gagcatctcc taatgtgtga acaaattcgc caaccgctaa acctgtttca
    44281 tgtgcaatta ggtgagtcag caacgcataa cttgcaatgt tgaacggaac gcctagaaac
    44341 acatccccac tgcgttgata caattggcag cttagccgtc cctcttgaac ataaaattga
    44401 aacatcgtat gacaaggagg caaagccatg ctaggaacat cttccggatt ccatgcagaa
    44461 acgatcaatc ttctagaatc aggagtcttt ttgattgctt cgatcacttg tttgatttga
    44521 tcgataaagc caccgtcttt tgtttcccag tgacgccatt gatagccgta gatgtgtcca
    44581 agatcgccgt atgtttcagc aaaggaatca tcggttagga tcttctcgca aaaaagcttg
    44641 ctctgctttt tatattcttc attgaattca ggatctgtca aacatctatg gccaaaatca
    44701 gtcatatctg gtccattata gtctggactt ttgatgtagc gttcaaatgc ccattcatcc
    44761 caaatatggt ttttattttg taatagatag cggatattcg tatctccttt taaaaaccaa
    44821 agcaattcgc ttttgatcaa tccaaaaggt actcgtttcg ttgttaacaa aggaaaccct
    44881 ttttgcagat caaaccgcat ttggtatccg aataagctta atgtgcctgt gcctgttctg
    44941 tcttctttac gatgcccttc ttccaacaat tttcttccta agtcaagata cgcttgttcc
    45001 atcctttttc ctcctaatct tctacccatt cactcaagta ttcccaccgt gccatttttt
    45061 cttcaagttg tgcttctgtt tcagatacgt cgttttgcaa ttcttgtaat cgagtgaaat
    45121 tatctccttg atgattcatc tcttcttgga gcaatgagat tttttcttca agttctgcaa
    45181 tctcatcttc aatcgtttcc cattcttttt gttccatgta agataatttc ttcttttcag
    45241 caggttcctt gctctgtatt tttggtttcg ctttttcagc aggctgctgg gtctcttttt
    45301 gcttcagtaa ataactggac atgtttccgt aataaagttc ttgttttccg tttccttgga
    45361 atactaataa cttatctgct accttgtcca agaaataacg gtcatgggaa acagtgatca
    45421 cagcacctgg aaaacttcta aaataatctt ccaaaatcgt caatgttgct atgtccaaat
    45481 cattggtcgg ttcatccaat aataaaacat taggttgctg aatcagcagt ttcaacaaaa
    45541 ataaccggcg tttttctccg cctgaaagtt tgcctatcag tgtcccatgc ataaatcttg
    45601 gaaataaaaa tcgttcaagt aattctgcta ctccgatttg actgccatct gctgtttttg
    45661 cttcttcagc agcttcttgt aagtaagcaa tcatccgttt atctggtgcc atttcttcat
    45721 tttcttgtgt ataatacgca agatgaaccg tttcaccgat agacattgta ccgctatcca
    45781 ggggaattct gccagctaga atattaagca atgtcgattt tcccgcacca tttttaccag
    45841 taatacctag tctttcccga gattgaatca ataaatctaa atgttctagc agtgtttggt
    45901 catttatacg gtaactgcca tctttgattt ccagtacttt tttccctaaa cgttgtgtcg
    45961 ctaggttcag ctctaaatca tcttcttggt ttacttggta caagttctcc tttagatctt
    46021 cgaaacggtt gatccgtgct tgctgcttgg tgccacgtgc ctgtacgcca gccctcatcc
    46081 aagctaattc ctgtttataa agccgcttgc gcttttcttc ttgttctact tctacacgat
    46141 cacgttctgc tttttctaag acgtaagctt cataatttcc tttatactcg tataattttc
    46201 caaatgaaag ttcgaagatt cggtttgcta ctctatctag aaaataacga tcgtgggtaa
    46261 ccatcaatag tgcgccacgg tattgtttca aatagttttc caaccactcg atcgcttcat
    46321 aatctaaatg gtttgttggt tcgtctagta atagtaaatc gggttcatca atcaacactt
    46381 gcgccagact aactcgtttt ttctgaccac ctgataaagt actgattttt ttatctaatt
    46441 cactgattcc taatttttgc aaaataatct ttgcgttggt atctgtagtc caagcatctt
    46501 ctttattcat ttgttcttct gctttagcat aacgtttttg cacatcaggg tcttctccgt
    46561 tttcccctag ttcgatcaat gcttcctcat atgctcgaac agtttggatc aaggggctgt
    46621 ttccttgaaa gactgcttgg agaacagtta attcatcgga aaattcagct gcttgagaaa
    46681 gataaccaat acggtaatca gttggataaa acactgtttg ccgatcaccg tcgctgctat
    46741 cgataccggc aataatattt aagagacttg ttttacccgt cccgttcgtc ccaatcaacc
    46801 cgatacggtc tttttcgtgg accaaaaaag aaatctggtc aaacaaatct ttttcaccat
    46861 aagttttttt gaggtcagtt acttttaatt ctttcaacat gttccctcct tatcttggat
    46921 attgtatcat aaaaatagtg aacttcagga aaattttttt ggtatttgtt ctttttgtgt
    46981 tctcaaaaaa agaacaggac cgattcgatc ctgttctgat atgttttgtt ttttataaaa
    47041 cttgaaaatt tttacctttt tattttattg gactcgtttt ttttgacttt gctcgaaacg
    47101 caaatcggaa agaaaagtta tcagatggtt tgttcatcgt ataagattct aacaaattga
    47161 tattcctgcg atagctgttc gcagaaaacg tcgtgatttt ttcagcaact tcgtattcat
    47221 caatgatttt tctttcaaga taataacctc ttagcatttt tttcgtaaac agactatctt
    47281 gctggatcat ccacgaagaa atcaaattcc ccgagttcat ttttattcct tcaagtttgc
    47341 gttgctggat aaaaaattga accatcgttt catcataaat atcagtcaag ttatctaaac
    47401 tttcaataat caactgagta tttttttgat atgtctctct gatttcttgg atatcttcat
    47461 cactaataaa tgtctgacga tcagcatatc ttctttgcca aaacattttc ggatggagaa
    47521 cgattcgcat taacctacgg acaacaatga accaaaagcc gataaaagac aaaatctggc
    47581 ttgtgataga atgttcgaat cgagaaagaa aacgggagta aaaacgataa ccattagaag
    47641 aaagcttgcc cgaacgataa ctttcatcca atccatcccg ttcgatcgac aaaatcagtg
    47701 cttgtatttc ttgtacttct tgcttttcat catcggttaa atcttccaag tacagatcac
    47761 gtatccgttc ttgataattt tctatcactg cttcagtggc taaaaattct tttgtattac
    47821 gatcaatttc ctttatttct tttcgcaaaa cttcaacgac ctcttctaaa atcatcaaag
    47881 cattcaaatc cgtgcttttc gcttcctctg tttccgtcag cataggcaag acaatgatcc
    47941 ctatgaccaa agtcactaag atgacacccg ccgtcagaaa caaaagcaaa gaccgctggt
    48001 aaaacatcat attattgatt gaaaatggca aaataaaaat cgtagccaaa ctgactgttc
    48061 cctttacacc gccaaatgtt aagatcaaga tctcgttcaa ctgttttttg aactttttac
    48121 tgccatcttt gaatacataa aaaagactga tagaaatgaa tctgatcaca aaaagcatga
    48181 cagagattaa tacgataatc gctaataaaa gaccgtttgg atataaaccg tctccccata
    48241 ctggcgagaa cacttgcgtc aattcgattc ctaaaaatat aaatactaat gcattcagtg
    48301 taaaagcaat agttgtccat gtactgtggg acaagctcga caattctgca tcaaacacag
    48361 agattttcct aaaaccagaa gcttggagaa tacctgctgc cactgcagca atgattccag
    48421 atacatcaaa tgcctctgcc aatacatagg ctagaaaagg aagcaaaagt tcgatcagca
    48481 gataccctgt gacatcttgt gctgatgctt tttcgatcaa atgaattatt ttttgtttga
    48541 accagacaag caaaaagccg atcaaagctc ccccaatact agaaactact agactcatcc
    48601 ccgcgtttac tgctgaaaaa gatccggtga tcagcgcacc aagagcaaac tggaaggcag
    48661 taacaccaga tgcatcattc aaaagacctt ctccttccaa aatatgcatc gcctttttag
    48721 ggatattcac tcgtccagaa agagaactga ctgctaccgc atctgtcgga cctaacgctg
    48781 ctccaaaagc aaagcaggca gcaagtggga cacttggcaa caggaatgat aacgtcgcac
    48841 ctaccccgac aagggtcaaa atgactcctc caaatgccaa gaacaaaatt gtcccgaaat
    48901 ttttgagaat cgaagaaata tctgcttttt ctccttctct aaataacaat ggtgcaataa
    48961 tcataactaa gaacaattca ggttcaaaat cgattgattg tccccactct gtcagaccta
    49021 ataccactcc aaggaaaatt tggatcagtg gagctggaat cattggcaaa atgcggctag
    49081 ctatgttcga taaggtgatc gttactgcaa atacaataac taactcagca aattccatcg
    49141 tccatcccct ttatttttta ttttgtgtaa gcatttttaa aaattgttct tttttcttta
    49201 aaattttggt ttcgactact tcaacctgtg tttggttccc ctctaattca cactgtacac
    49261 gttttgactc caaacgaagc agatcatgtt tcagacgttc caactgacga tacatcaata
    49321 tatttctttt acgcaactta caaagtattt gacggcggtc atccatcgaa agggcgccat
    49381 agcgatcatg caacagtttt ttttgttgtt caaaactctc tctcattttc cagatctcct
    49441 atacacaaac ttccatcatt atagcaaagt tctttttgga tgaaaaatat ttcattgttt
    49501 catcgtttat tgctagttag aaatttccat aaatttttca acgcctcatt ctattccaaa
    49561 tagtatcaga tatcaagtaa aacttaagaa agataaaagt ttctgacaaa aggatgttta
    49621 ctttcccatt cctgcctttc ttgttctttt atcacaacaa aaaaatgggg ctgcgacaaa
    49681 aatcttgtca cagtccctta ttctgaataa acggtgtgga agagacagcc tatcagtagt
    49741 aataagacac atctctttca cagcctcatt tttgcgtaaa aatcgattta ttcgaaaaat
    49801 tccccgtata catcttctac acgttgatct ggattgatca cgatatacaa gttacctgtt
    49861 ttttcgctga tttttgttga ttgatattga ttgtctaatc cttctgttcc tttatctaga
    49921 tatgggaagt acacaagatc aggtgtgcca tttcggtaaa ggatttccat tcgctcgata
    49981 tcttcgtatt tcaatgcgcg attaaacatg cccatttcca gtccgcctag gttgatatca
    50041 tgtgtcgcaa ctgcgtcccc ttcatggaag atttcgattt taaatccagc acacggatgg
    50101 atttcaacaa attcactgcc attgattcgt ccaaaacttg ttgtgatttg tttgatccaa
    50161 agatcgccga tatgacggcg gttgatcgtc caggtttctc catttctaaa ataaaatttt
    50221 agcgctgtca tttctttact cattaaaaag actcctttat gatgtaattt attcttgtac
    50281 tacctcagtt tagcacaaat ttatgtaagt ggtcacatct tatgtttttt cttctaatga
    50341 cgtatctgcc cactttaagt agccaacaaa gctgagcaga tacagtctag tgttttttaa
    50401 atcaattttt ctttcgataa aaaatccaaa acctgttttt tatcattttt tattctttgt
    50461 tctacgacgg ctttttcagc taattgcaaa gcttcactaa cccacttacc aggttttttt
    50521 ccagtttctt ctagcaagat tttaccatct actgctaatt cgcttaatga cttgataggt
    50581 aaacgatcat aggattctcc tacggcatcc atctgaggtg tttcgtcaaa atagatcatc
    50641 aattcttcta cttgcagtgc atgttttttg cctaagaagt acaattcgaa tggttccaaa
    50701 gctccagttt ctcgttgtcg tagtccttgg acaagtgctt gaacgtcttt aatcatttgg
    50761 ttagaacact tccacgattt caaaaaggaa cgaatctcat tttcttttag atccagttgg
    50821 ctgatcaata atgcccatgc ttggctctct tctttcaatg ggacggatgg taattcagca
    50881 aaacgtaaaa gtgcttcgcc ttcttctttc aaaccgggac agcaaatgta gcactctgtt
    50941 tcaataaatg catggattcc cgcggcccgt tcatttccca agagcatctt gatgaattcc
    51001 acatttatac gttctacaga tattttacct aatagctcgt ggttttcttt gatggcgtag
    51061 aaggtttctt cctctaattt gaaccctagt tgactgacaa aacgcaaccc gcgcatcatt
    51121 cgtaaggcat cttcatggaa ccgctcatga ggattgccta cagctcgcaa gatctgttct
    51181 tctagatctt gcagaccatc gaacaaatcg atgatttctc catcttcttt caaagcaaac
    51241 gcattgatcg taaaatctct tcttttcaaa tcttctgcca acgaacgaac gaattcgaca
    51301 tgatctggtc tacggtagtc ctgataagtt gattccgtcc gaaatgtggt gatctcatat
    51361 tgttcatcat tttctaatac caacacagtt ccatgttcga tcccaatatc tatcgttttt
    51421 ggaaaaagct gtttgatttc ttctggaaat gcgcttgttg cgatatccac atcatggatt
    51481 ggctggttca gcaagatatc acgaacactt ccacctacaa aataagcttc atatcctgca
    51541 ttctctaatt ttttcagaac tggaattgcc ttttggtatt ccagaggtaa ttgttttaat
    51601 ttcatttttc caacctttcc tgcgtgtttc ttgtcctgag ataaagactc agttgcatgg
    51661 tgactgtttg acaagtccgt ttcagagtct tcaaggccag gaccacttca tcttgtttac
    51721 tcgtccttac gctcaaagcg attatggtct ctttgattga atttttccat gtttttttca
    51781 aaaacttcag ttaagtcgat attcaatgaa ttagccatga tcatcgtaac gaataaaaca
    51841 tcaccaagtt cttctgctac cgagttaact ggttcagaag cctttttgct tttttctcca
    51901 taagtgtggt tgacttctct tgccaattca cctacttctt cagttaatcg agccatctga
    51961 gctaatggag agaaataccc caccttgaat tgctggatat attcatccac ttcctgctgc
    52021 atactgtata aagaacgctt tttatccatc tttttccctc caaaactatc ttaccaaaac
    52081 tttaacgcaa atcctagata agagcaattg agaaaatcga atttccatgc tacatttagg
    52141 aaggaatttc taaatggaaa ttctgtgaaa atacataatt gaaagctgga attcatatgg
    52201 tcaaacgaaa aacagtaaaa gaagttcttt ttatcattat aggaacaagt atttatgcat
    52261 ttggtcttgt ttacttgaac atcgctaatc atttagcaga agggggagtc tcagggatca
    52321 ccttgatctt aagagcttta tttggtattg atccagctta tacgacttta ttaatcaaca
    52381 tcccattgat tttactagga ggaaagatcc tcggtaaacg ttccttggcc tatactgttc
    52441 taggtacact ttctttgtca ttctttttat ggatctggca aagggttcca ctagaaatca
    52501 atttacagca tgatctactg atcgtttctc tacttgcggg tttagttgca ggtgtaggaa
    52561 gcgggatcgt ctaccgcatg ggtggaacaa caggcggcag tgatattatc gctcggattt
    52621 tagaacaaaa ttacggcatc actatggggc gttccctatt ggcatttgat atcatcgtct
    52681 tacttgcttc attaacctat atcgacttga aacgtatgat gtatacgttg attgtttcgt
    52741 atgttttcag tcgtgtgatt gattcgatct tagatggcgg atattctgca aaaggtctac
    52801 ttgtcgtctc caacaaaagc gaagagattg caccactact gatgaccggt ctgcaaagag
    52861 gtgtgacttt cctttctgga gaaggcgcat tctctggtaa ttcaaaaaaa atcatctata
    52921 tggtcgttag ctctagtgaa ttaactgaag ctaagaggat cgtccacgag attgacgaaa
    52981 aagctttcct ttcaatcatc aatgtgcatg aagtggaagg gcaaggcttt acttatttga
    53041 agccacagac aagattactg aaaaaactat aagaaaaaag acaaagatag gctatgagga
    53101 aagattccac atccagctga cctttgtctt ttttagtact atactatttt taactaattg
    53161 ttatgttcac ttacccggtt acttttcttt tgtgtctgca aaaggatctt tcggttaact
    53221 tcatcagata atttcaaaaa agcatcccta tctccatctt ctagtgcttg atcgatttga
    53281 gcataaagca tttttagttt tgcctcttct tctttttctt gaaaatatcg ttcaattaat
    53341 tcagtatctt cttcactgac tgaagaatcc caagaagaaa atggattgtc ttctaagata
    53401 gatagatatt ctcttgtctg ccaagcattt tcgaatatac attccacata taaaggcttt
    53461 ttccaattca aacggatttc atgaaaaatc tggtctgtgt ccgtaaattc ttttccctgc
    53521 aaaaacaatt tcatgggttc cccttcaaca gatagatcac tgatctgcag tcctcgatcc
    53581 gcaaaattcg ccccttcaac aaagtggaca ttgcttaaaa tcgcttcatg attggcaaga
    53641 taattcaaga tccaaaaaac ttctcttcgg ctaaaagata catgattgac catccacgtt
    53701 aaaaaattct tcttttcccg tacatcaatt agcatttttc tcacctctct gttaggtcat
    53761 ctaataatga cagtgcctcc atatctcctg gttcaagtgt taaataataa gtcaataatt
    53821 cttgcgctct tttcagctgc ccttcttctc ttaagaaaaa ggcatactct ttcaagaaat
    53881 ctggttcatg tgatagttca tgataagcct gttcatagtg tacagctgca acggtaaagt
    53941 cttctaattc attataagca tgggctaggt tccattctgc ataaggatta gcagtttctt
    54001 ccatctgatt gatcacttgt atcacatctt catatctttc atcatccaag taaagattac
    54061 ttaaagtaag taatgtttca tcttgtttgt ctcctacttc taaagctttc agtaaaagtt
    54121 cttctgctcg ctgtctatca tgcagacgga aagcattttc cgaggccagg tgataaagtt
    54181 cgacttggta aggattttct ttgattcctt cttctaacac tgtttgtgct tcttcaatca
    54241 tttcttcttc ttgaagagct tgtcctagat atagatataa cgattgataa tgcggattaa
    54301 cttctcgaag ttcttccaga tagttgattg cctgctgatt ttctttcaat tgaagattcg
    54361 taaaagctag ctggaacagt cgatcatctg tcctttcttc tttcagtgct tcttctaaag
    54421 gtacgatagc tgcttcgaat tctccttgca tacttaagga ttgtcctaaa cgttcctgca
    54481 tcgagattcc tgaaatttca tttatcccgc ttgccaacaa tgtttcataa accactgctg
    54541 cctctttgaa acgatcaact gaaaaataca attcagctag cgcaaactgg atcaatggtt
    54601 catcaggaag caacccagct gcttctttca attttgcttc actcacttca gggattccga
    54661 taacttggta caaatcagct atcgccaata agctttgcgg atagtaatca ctttgcttat
    54721 ccactttttc caggtattca aaagctaagt cgatctcatt atcttcgatt gcaatttctg
    54781 ctaacgggat gttcagtccg tcattttctg ggtattggtt gatcagttgt tcaaaaatct
    54841 gttttgcttc gatcaaaaag ccaatagcca gcaattcttc gcctagttca gctaatatat
    54901 ctgctgggtc ttcttttaat gctttttcta aaaagatttg tgcttgtgac aagtcttcat
    54961 ctgataaagc ttgcaacatt ttttcgctat ttgtattcat ttcaattccc tcttatctca
    55021 ctgacgattt gttgaaatac cgtcattgtt ctcaataatt ctttttcgtt tggatgttgg
    55081 tatccgtcta ttatacctat tttttctaaa acaagtattt ttttgctttt ttctgccaaa
    55141 aaaagaactt tttccaaata ctccatcttt gaaatctgtt ccagccagtg gattggatag
    55201 ccaagcaagt ccagccacac aaaaaagttt tttagttgga aatcaaaagg atatgcttgg
    55261 agattccaca cgaaatgaaa catcattcct aaaaattttt tcatactagc agaaagtaga
    55321 tggccatttt cggtcagata aaaagcttgt tcaaacagtt taccataatt tgacaggttt
    55381 tctgcctcta attgataaaa atagatcatt tcttcgatca tcccggcaaa cggccgagtc
    55441 gttaattgct ttttatttgg atagttttta tacagatttt gcaaaaaacg atgatcacaa
    55501 atcattccgc aacagataaa agtcaaaaag tcaatctgct ttccgtccac ctgccgctcg
    55561 ctgattgttt gatcataaac gatgcgttct ggcagattga tggtttgcag cactacacga
    55621 tttggtagct gcaaaatcgt ccgctgcggt gttaatgctc ggcttagaga acggatcgat
    55681 acagggatca tccatagtga tgaatcaagg gttgtatgtt tttgaaaaaa acctgccaga
    55741 tccatgatcc cttcatttcc aaatccgatg ataaacatgg attgattttc gggatagcgt
    55801 ttgacgaaat caagcaggtt ctttaattct gttaaatgat tgcattgagt gttggcacaa
    55861 atataccagt cattgtttgc ttgatttgca aataacgggt tgatcttatc tgcaaacaaa
    55921 tcataatagc gctgattcgt taaaaataag attggctgat caatggaaag ttctcttagc
    55981 tgactgacta gcgtttctcc gtaaaacact ttcgtcaacg catcttctac ttgataatca
    56041 agttccatac gcatccctcc tcttcttatt tttattttac cattagtaag caaattctag
    56101 aaaagataag agcaaagctc acctcataac ttgcaaataa ataaaaatta aataaaatac
    56161 atgtgttttc tatgtatgac atacggattc ggaatctctg tctcttattt attccttttc
    56221 tgctaggaaa taaaaagaaa agcacttgta gccctagagc cacaagcgct ttttcaactg
    56281 tcttatttaa cagcgtcttt taatgcttta cctggtttga atgcaggtac tttgctagct
    56341 ggaatttcga tttcttcacc agtttgtggg ttacgacctt tgcgagctgc gcgttcgcga
    56401 acttcaaagt taccaaaacc gattaattga actttttcgc cgttagctaa agcatcttga
    56461 atagttgaaa atacagcatc tactgctgca gttgcatctt ttttagttaa atcagtagct
    56521 gaagcaactt tttcgattaa ttctgctttg tttgccatgg atagattcac ctcctccaca
    56581 agagatatga tagaaatatc atttagttaa aactttctga gtggtccaga agtttaaaaa
    56641 gaaatattga ttaacttcaa tattctgtta tcacgatatc atatgaaccg cttaatagca
    56701 aggtttttac caattttcac atgctttttt tagtcataga aacgtaacaa acgaaactgt
    56761 ctttttcttt tgaaaaaaaa acgtcgatat ggtaaaatgc ctattttctc ctacgaggga
    56821 taattcggat ggacgtccct tcaaaagtaa atgcttttct gatttgattt tctaagaaac
    56881 gttcataaga aaaatgcatc aattcctctt cattgacaaa aacgacaaat gtaggtggtt
    56941 tgacagcgac ttgtgtagca taaaaaatct tcaggcgttt tcctttatct gttggtgttg
    57001 gattgattgc tacagcatcc ataatgatat cattcaacac agccgaagag atacgaagat
    57061 tctggttcat acttacttgt tcgatcattt caggcagacg ttccaaccgt tgtttagtca
    57121 ctgcagaaac aaaaacgatt ggagcgtaat caagataccg gaattcatca cgaatctctt
    57181 gttcgaaatc acgcattgta ttggtttctt tctttacaag atcccattta ttcacgacga
    57241 taataattcc acgaccagct tcatgggcgt aacccgctac tcgcttgtct tgttcccgaa
    57301 tcccttcttc agcattcagt accatcaaaa cgatatcaga acggtcaatg gcacgcattg
    57361 cacgcatcac gctgtatttt tcagtatttt cgtatacttt tccgcgttta cgcataccag
    57421 ctgtatctat cattaagaat ttctgaccac tttcagaaac gaagtgggtg tcgattgcgt
    57481 ctcgggtcgt tccttcaata tccgaaacaa ttacacgttc ttctcctaaa atcgcattga
    57541 tcaaagagga tttaccaaca tttgggcgac caatcaaact aaacttaatc gtgtcgtcgt
    57601 cctcttcttc agtctcttca gagaaatact tcactgcttc atctaaaata tcaccaatcc
    57661 ctagtccatg gcttcctgaa acaggataag gatcgcctag ccctaaggaa taaaattcat
    57721 agatatcgtt tctcatttct ggattatcta ctttattgac tgctaaaatc actggtttat
    57781 tacttctgta taaaattttc gcaaccatct catctgcgtc tgtcacgcct tcgcgtccgc
    57841 tgactacaca aatgattaca tctgcttctt caatcgcgat ttctgcttga tgcttgatct
    57901 gatccataaa aggttcatcg ccaagatcaa ttccgcctgt gtcgatcacg ctgaattctc
    57961 gacctaacca ttcacctttt gcataaatac gatcacgagt tactccagga gtatcttcga
    58021 caatcgagat acgctcacca gcgatgcggt taaaaatcgt agacttaccg acgttcgggc
    58081 ggccaacgat tgcaattgtt ggatttgcca tattatttcc tcctcacaag tttccaatct
    58141 cacttagttt aacgaatgca ttgccaactt tcaagcatag attgtcaatt aaacgaagta
    58201 tttttattcc ttattgaaat actaatcgct tttcaaaaga aaaactgaga caaaagcagc
    58261 tgctttcatc tcagtcacag atacaaaatc aattattttt cagaatcatc ggttgctttt
    58321 tcttctactt cttctttcaa cgcatcacct aaaatgtctc ccatagtaaa tccagtattt
    58381 tcttctggta attcataaga ctcaacattt tttggttctt cttctgaagc tggtttttct
    58441 tctaaagctt tgatactcaa tgcaatacga tgttcttctg gatggatctc taatactttc
    58501 actttcactt cgtcaccttc atgaagcact tcatgtggtg tcgcaatatg tttatgtgaa
    58561 atttgagaga tatggactag cccttctact ccagggaaca cttcaacaaa ggcaccgaag
    58621 cttgtcaaac gtttaactgt tccgtctaat actgtgccaa ctggagcttt tgtatcgatt
    58681 tctgaccaag gtccaggctg tgtgtctttg attgacaagg aaacacgttc ttgttcagga
    58741 ttgatagaca acactttgac atttacatca tcattgacag ccaatacatc acttggtttt
    58801 gctacatgac tatgtgaaat ctctgagaca tggactagac cgtcaattcc gcctaggtcg
    58861 acaaatgcgc caaaatcagt aagacgtgca acacgtccac tgagaacatc tccctcatgg
    58921 atagttgaaa gaatttcttt tttcttttct tctctttcgt tttccactac cgctttatgc
    58981 gataagatca aacgattttc tgatggttcg atttcgatga ttttaaagct caatgttttg
    59041 cctttatagt ctgaaaagtc tgcaacaaaa tgatcttcca ccattgatgc agggacaaaa
    59101 ccacgtacac cgacgtcaac gactaaaccg cctttgacaa catttgtaac aggtgcttcg
    59161 atgaccttgc cctcttggaa atctctttcg atatcttccc aaactttttt agcatctaaa
    59221 cggcgttttg aaagtagata gctgccgttt tctttatctt tgccgatcgt actgataacg
    59281 actaaatcta atacatcacc gacttttact acttcgctga tatcttctac cggcaaagtt
    59341 gataattctt ttgccggaac gactccttca actccagccc cttcgattcc gacaatcact
    59401 tgtttgtctt caatgactaa aacttcgccc ttaacaacat cgccaacttt tacttcttgt
    59461 acgcttttca ttgcgtcttc cattgactgg ttgttctctt gattttgatc aaattctgtc
    59521 ataacacttg tcctcctaac caacattctg ccgtaagtac ggtactgttt ttagtttaat
    59581 tgattattca aaaaaaataa agcgatttcg ttcgttttcc ttgaaaaaac aattgttttt
    59641 catttttttt cttaatttaa aaaagaagat aacctttttt caaaacaaca gcttttattg
    59701 ctgcaacgac ttctggaatc gatttgccag tcgtatcgat acgaacagca tcctctgctt
    59761 gcttcagcgg agagacgtcc cttgttgaat cataatggtc tctttcctgt atctctttcg
    59821 tcagtgtttc aagatcagtc ggaattcctt tttcttggtt ctctttgaaa cgtctttctg
    59881 cacgctcact tacgcttgct actaaaaaga ttttcacttc tgcattcggt aatacagctg
    59941 tcccgatgtc tcttccgtcc atgacaacgc caccgcgttc ccctattttt cgttggacag
    60001 caactaattc ttctctgact tttccatgtg cagagacttc agaaacagct tttgtgacat
    60061 ctggttgacg gatttctaaa gtcacatctt ttgatgctat gaatacatgc tgtccatctt
    60121 cttgttgcga aaaagagata ggatactttt tgatcagcga aactagccct tcttcatctg
    60181 aaaaagatac attgttcgta atagctaaat aagttactgc gcgatacatg gcaccggtat
    60241 ctgtataaat ataattcaat tcttttgcta atatcttggc aacggtactt ttcccagatg
    60301 aagcaggccc gtcgattgca atactgattt gtgtcatgat ataactcctt tttctcagaa
    60361 aaaaagccgc aaagcgcggc tattattatt taattcttaa ttcttgacca gggtaaagca
    60421 taaagttgtt tggatctaag ccattcaatt caaacaattg atcagtggtg atccctgcac
    60481 gctcagccac ttgttttgga ccttcgcctg cttgaacagt cgttgttgaa ccggctgcag
    60541 ctgatgaaga aggtgttgtt tctgctgcat tgctgcttga atcccctgct tgataagttg
    60601 cctcacttga ggaagtcggt gttgtctctg ctgcaatact gctttctgac gttacacctg
    60661 tatcttgaga cacagtagat tcttccgcga ttgtactaga ttctactgtt gaagtctcag
    60721 ttgactcctc ttttgttgta cttgtagaac tttctactac tgatgtcgaa gtttcagaag
    60781 aagttgctgc tgttgcattg ttgcttgatc catttctgat ccagaaataa gcacctgctg
    60841 gaatagcgat acataagacc agtaaaatca aaaatagcga aagaaataat gtatttcctt
    60901 tcttttgttg tctctgagaa cttcttgatg aaacttcatc atctgtatcg tagattggtt
    60961 gttcccatgg ttcttgtgtt tcgttatttt ctgtagattg atgtctatct tttctgctca
    61021 ctgttttacc tcctaaactc tatcgtgtgt attataccat aggaaaaagt gtcttgtcct
    61081 aaaaaatcaa tgttccatgt tttttttaaa taaatttagt aaaatcgttc cccaatgaag
    61141 ttgttcagtc ttagaaaaaa gcttcctctt tggcagaagt tctgcttttg ctccatcaat
    61201 atcacaacaa ttctgcggct tttcggagag tttttcgcca aaataagcta agatgaattt
    61261 tctgcggcag ttttcttcat taatatagcg aagcatctgc tgcaggcgga attctttttc
    61321 tttttcctga cgtttgagcc gttccaccca attttctggt ttttcagact gctggatcag
    61381 ctccatccat tttttttgca gctcatcaaa gggagcttgc tctgtggcat attccaaata
    61441 aagctcaaaa ctttgtcgct gttctctaga aagttgatta aagaaataat ggatacgttc
    61501 atccccactt tgataaaaaa gaattgctgc actttcttcc tgatctctgc ctgctcgacc
    61561 gatttcttgt acatagtttt ctagactgtc tggcaaatca taatggacca cataacggat
    61621 atcagattta tcgatcccca taccaaatgc attcgtagca atgagaaact gcagctgatt
    61681 ttttacaaac tgctgttgaa gttgtcttct ttgagctgca tcaagaccac cgtgatagta
    61741 tccaactgta aaacgttccc tgaataaatg atacaatcgt tcgacttctt tttttgttgc
    61801 acaataaatg attgccgctc cttgtgctct ttccatgaag tgttcaagtt cttgttcttt
    61861 ttgctgcgtt ttccgaacaa aaagcgagat attttgccga ttgactgatt gtctgatctc
    61921 ttttggtggt cgatcaaata gtacatgacg tatatctctt gctactaaat ctgttgctgt
    61981 cgccgttagt gccagtgtga ccgggtttcc taaaagcttt cggatttttc ctaactgttg
    62041 atattctgga cggaaatcaa ctccccattg agacacgcaa tgagcctcat ctactacata
    62101 taacgcaata ttttgttttt tcagatgctc taaaagagaa ggattagtca acatttcggg
    62161 actcaaaaac aaaaatttat attgcgatag atgacggagg acgtatcttc tttcagattg
    62221 cgaaagtaaa ctattgaaag cagctactcg tttctcccct cgtttttgta attgcgtcac
    62281 ttgatcttcc attagagaaa gcagcggtga cacgatgatg accagacctt ccattaaata
    62341 ccccgtcaat tgatagcaaa ggctttttcc cgttcctgta ggcaaaatag cgagagtatc
    62401 ttctccatca agcaacgcct gaataatttc ttcttggccg ggacgaaatg aatcaaatcc
    62461 aaaatatttt tttaattcat ttattaacat tctgtatcgc ttcctttctc aatagataga
    62521 tttgatacaa acgaaattct ccataatcta attgtccatc tacattccat tcgctaaagc
    62581 gccactctcg cacggaatcc ttatttggtt ccaaacgttc gattgtttct gcagagagaa
    62641 tccactcgta tggaaaatca tcaaaaaaca aggcccactc aataagatga tctgttactg
    62701 ttcctctttt taaatgacgg attgccatga tctggtcaat tgtctgacca ttcataaaca
    62761 tctgcttggt gatcatcatg ctttgattga aattttgctg taataatgaa tcgatcaaag
    62821 cgtgccaaag cgaatctggc cgtttttcga tttgtgctaa caaaaggtgg atacatctgc
    62881 tttgttgcag ataaaccgct gactcatcat tgttttttgc cagctgataa ggaagcagcc
    62941 ctgttcgctc atttccagat aattggttag ctaaaaaatc tgctgcttct tctggtatct
    63001 ccgaaaacag ctgcaccaaa ctctcatacg cagtatcaat caaaatttca cgtggcaatt
    63061 ggctttcact aagccatttt tttagttgga aagtataaaa aggacttgtc tctgctggaa
    63121 gatagtcttg aatacccgtt gccagattag aaattacttg aacagcaaat ttaatgagcc
    63181 gccaactagt ttcccctgtt ctgccatagc ggtcaaaacg caaaccagta tgttctattt
    63241 gatgttcgga caacatatca gcccctgcag gcgttagctt tgcttcattt tcatttattt
    63301 cgatccaacc ttgctgcgca attttttgca aaatttgata aaacttatcc tgcttcaatt
    63361 ctggaaaact gttatggata aacaaaagtt cgtgggtaaa tccataaata agtactgaag
    63421 tcgttcgttt tcccacaagg agttggtaaa gagaactaat ccgcagcttg tgtcctgatg
    63481 aaaatagcga taaaataaaa acaatcatat tattctcctt aagttaggat ggttagaaat
    63541 gtcactacta tgtaaacttg tacctgaacg ctgtattgca tgcggtcttt gtcaaatcga
    63601 agcgccagat attttcgatt atgatgaaga aggaatcgtt ttatttgccc aagaaccaga
    63661 ggctcatcaa cagttcgtga tgccggaaga agaaacatat gtaaaaaaag cgtatcaaaa
    63721 atgtccagtt cgagcgattt tattagaaaa taaaatataa atataaacga aactttaaaa
    63781 acgtacacta agttctctaa acttgaaaat aagccgggat ctgtttctct tgttccgtga
    63841 aatcccgact tattttgaaa gattcatttg ctatctatag ttaattgact agttttttca
    63901 cggaccctat aggacccatt tatattattt caactttttc agccagtttt ccaatgtatt
    63961 gacgatcaca gtttgctggt ctgcgttaga aatttctgcc tctccatctc ctttttgctc
    64021 accatagctg ccaaaacctc catgatttcc gccattgatt gatacatact ctgttctttc
    64081 cggtaaatac tgcttagctt cttgataagc ttcctgattc aatactttat cttttgtcgc
    64141 cgtgatcgat aaaacaggaa cagctgattc tgtcaatgtt ccttttttgt cgggataact
    64201 tgccatgaag aatacccctt ttaaatgttt gtctgaatga ttcttattga cataacgact
    64261 tgccattacg ccacctaatg aatggccacc gatcacataa tcggaatttg gaaaatcttc
    64321 tttgacctta tccgctttgt ttccgcccat cactgctaga tcaagtggca ttttgacaat
    64381 agcgactgaa aagccgtttt ttgctaaatc ttgcgcccaa atactataac tttctggctc
    64441 tacgagagca ccaggataaa aaataagtaa tggagacttt ccatgattct ttttctctcc
    64501 ttcaaaaaat aagtaatctt ctttgttttt tccttctttt accaacactt gcgcttctga
    64561 cgatggttcg tacgtatttt gctgtatata gtaataaccg gctccaccga gtaatcctaa
    64621 aatgatgact aagccaatta gtattcgtct aaatattttc actttctccc accttttcta
    64681 tctgagtgac ccctttttct taatcaagta tgattatacc ttaaaaaaat aagagagaaa
    64741 tgcaaacttt ccagcatcaa ataaaaaaag acggaatatc catttgaata tccgcccttt
    64801 ttgtatgttt atttttattt agcacataaa cgatacacag cttctgcata aatttccatt
    64861 gctttataca tactacttaa ttcccatcgt tcattcgcct ggtgcataaa gtcaggagta
    64921 tcagggaaca tcgcaccgaa tgccacacac tggttcatcg tacgagcaaa agtagcgccg
    64981 cctgatacaa acggttcagt catgtctcca gtaatatcgc gatatgtccc caatagattt
    65041 ttcactaact cactgtctag tggcacgtat aatgaatcaa ggaagtcaaa ttcttcataa
    65101 gtcaacccat atttttctgc tgttttagtt aatttagcta ctaaatcatc tttcttgaat
    65161 gtaacaggga ttctcatatc tacaccgatt tttgtttgtt ctggtgtgat ttccaaactt
    65221 gcaaagttca tcgttagctc gccggattgt tcatcttctg ttttgcccac gacagcttca
    65281 ccagttgcat tttcttgaac taattttcct aagaaattga ttgggccaaa atcaaatact
    65341 tctgaaagag caattgccag acgagaaata gcattcactc cttgtgctgc atctttcgcg
    65401 tggatacttt taccaagaac gacgatgcct tcaccttgtt cttcgaaatc gaattcgtgt
    65461 tttttcaatg cttcttttac ttcagaaagc ttttctccag agtaaggagc tgcatcagga
    65521 acaacattca acgctcctcc agcttttaca gaaaactcgt tcgttcctgg tcctgtcaag
    65581 taagcttgca ataagccttt ttctgcataa attaaaggaa attcagcatc tggcgcaaat
    65641 ccttgcgtaa ttccttcttc tttttcgtta tatttttcta aacaacgcca taaattttct
    65701 tcatctgtac caaagataaa acgaatacgt gtcttgaatt ccacgccagc atcaatcaaa
    65761 gctttgacgg catacattgc cgcaatagaa ggacctttgt catcttggga tccgcgacca
    65821 actaaccagc cgtcttttat agttggatcg aatggttttg tttcccaatt attttcgtcc
    65881 cctgctggaa caacgtccat atgacacaag ataccgaata attcatctcc agagccaatc
    65941 tctgaataac cgtaatagcc ttctggatct ttaaatgttc taaatcctag cttttcactg
    66001 atttccaata ctttatccaa agcttcaatc acttttttac cgaagggatg tccttgtcct
    66061 gaatctgctt catctagtac ggaaggaatt cggatcaatt cgctcaatga ttctaatgct
    66121 tgttcatgat gttgttctgt cacaaatttt ttcatgatat tatcctccta atatgttaaa
    66181 aagggtcatg tcaaattcct ccgacacaac ctcttcttct caaataatga cgttttaagc
    66241 ctgctccatc agcaagcaga atgttcttaa caaatgatga aagaaaaaac actcactgaa
    66301 tggagctatt gacttatcca cgtgcttatc gatagccgtt tatcggtttc ttcattctta
    66361 tgatagacct tgtacctgta tttgtctagg tttagaacat tgctgctaca cctaggatca
    66421 cacagatggc tacaaataat atcgcaatta atttaccggt aaatttccac cacactgaaa
    66481 catcgaaacg agcaattgct acagcaccca ttacgatagc agatgttggt gtaattaagt
    66541 ttactaaacc agatgcagat tgataagcag tgattactaa atccttaccg acgccggcaa
    66601 attcacccat aggtcccatg attcccattg ttgcagcagc taagccggaa gttgaaggaa
    66661 tcaagaatga catagggata tagaaaataa atgtaataat gatgaataca cttgaagata
    66721 atgaacttaa tccttgttcg ccccaatgta agatggtgtc tgtgattaat ccattgttca
    66781 taaccacttg gataccacga gcaacagcta ctacgattgc tacaccgatc aaatcagaag
    66841 caccagctaa aaattcagag acaaattcag actctttcat gccataagtg attgctacta
    66901 cgatggacat tactaagaat aacatagtaa tttctgggaa gtaccagtca cctaatggaa
    66961 gcatgctttt acctagaacc gtacctagta ccggaaggtt tgttaaccag tttgtcaatg
    67021 attcaaaaat cgtgaaattc gcattcaagg ttgtccaagg aatcaaactg atgatcatga
    67081 taccaaaagt aataaagaac aatacattaa catgtttttg ttttttcgtg attacttctt
    67141 gacgaccgat tgcttcaata tcgaaatgct tcatatcttc tttacgttgg ttgtatacta
    67201 atgattttgt tggatctttt tcgatctttg atgcataacg atacacatat acaatagaaa
    67261 tagctaacaa aataacgaac atcaagaatc gaagaccaat accttcaccc atcgaaatcc
    67321 caacagcttg tgaagctacg cctgttgcaa aaggatttac cgttgaagct agacagccta
    67381 cttgagaccc tactaatacg attgctacag ctactactgt atcaaagcct acagccatca
    67441 tgactgggat cagtaaagca tagaatggaa tcgtttcttc tgccattccg taagtagagc
    67501 cgcctaatgc aaataacagc atcaatactg gaataagcat tttttctttt cctttgaatt
    67561 tcttcacaac tgaagcaatc ccggcatcta gtgcccctgt tttgttgata atacctaaaa
    67621 atccaccaac aaccaaaatg aagaaagaga ttgggattgc agcaggtgtt tctttagtta
    67681 gctcccccgc tggtacacct agcatcccat tgattggcgc catgaaaata tcccacagcc
    67741 cttgaggatt ttgttgtact cgttcatatg atccagcgac gatgttgcct gcatcgtcta
    67801 cactatagtg cccagctggt ataaaccaag taagtacagc gattactgca ataataataa
    67861 ataagaccgt atacgctgaa ggcatttgaa attttttctt ttttgtttgt tccatcttct
    67921 tcttcctttc ttttccgtta gagacaacaa ttttggctct aatgttattt tatcaaaaag
    67981 agtatcagaa caaaaataat aacacgcatt ttgtaaaact tttgttcaaa aagttcgttt
    68041 ttccctattc cctcctcttt tttcaggttt ttaaagcttt cttctttttc attgtagcgt
    68101 ttcctttttt ttataaacaa acattttcgt cttttaaaag gagaagaatt tcacatttgc
    68161 aaagaattga ttgattttta tgcataaatc aaaaaaatac tcaaaacaaa caaataaaac
    68221 gtttacatta ttgcatgtct tgtatttttt tccaaaaaaa aagaccaaga aatttcttga
    68281 tctttttgat atttatttga ctgctgtatg acgttgttcg aactgatgtt gttttcgact
    68341 taaccatgga agaagttgcg catgcagcac taaaaagaca gcactcacga tgaatccttt
    68401 gatcaagttg aaaggaacga tcccaatcag tacatagttt gctaaagaca tgcccagcat
    68461 ctgtgttgcg ttcagcccta aaaacattaa gtaaagaggc gtaataacga aataatttgc
    68521 gatactcata aaaatagtta gagcgatggt tcctgtaatg acacctaaaa atttgtttct
    68581 tcctttatgc tgtttcttta cactaaagaa ataataaatc gggaatgtaa agatcgttgt
    68641 tgataagaaa ctagcagtgt ctcctaccaa attatccgga gaaaaacctg tagtcagcag
    68701 atgtaatact gaacgcaaga acgctgttgc aactccggcc gctggaccga ataagaacat
    68761 gctgatcaat accgagatat cactaaaatc gatcttcaaa aaactaaaag ccggcatgat
    68821 cggaaaagca aaatactgta aaactaatgc gatcgcagca aacatcgcta ctgcaaccat
    68881 tttctgtaca cgggtgttct tcatttgaaa tcctcctgtt aggactcctc ttcagcgaac
    68941 tttttgacgt ttctctccaa atgaaaaaac tctattttcc agggaaaata gagttagagt
    69001 tatttgatca agcgtcgtca aataagctct atcttcttta tccagactat actgtcggta
    69061 tcggaatttc accgattcag ctattttttt gcaaaatagg tcgtggacta taaccaccgg
    69121 tcgggaattt caccctgccc ctgaagacga accttatata atttttgttt acatattcat
    69181 tatacatatc tattcaaaaa acgcaacatc taacacttat aaaaagtaag tgttcatttt
    69241 gagtggttca gcaaactgct gacttctttt ttgttcagtt cacggtaatc cccaggacgt
    69301 aggccttgta aagtcagatc tccgtatttt tcacgtttta gtttttgcac aggaagacca
    69361 actgcttgaa gcatattttt cacttgatga ttacgtcctt cgtggatcgt caattccacg
    69421 atactgtggt ttgtcttagg atccactgat aaaatttgga atttagctgg agatgttttt
    69481 cgtccttcga tctttattcc ttttgtcaaa ggtgcaagca tcgattttgt agcgatccct
    69541 tttacctttg caacgtaaac tttatccact tcatgttttg gatgtgtcag gcgctgagca
    69601 aaatctccat catttgtcag tagcaagatc cccgaagtat cgtaatccag tcgacctaca
    69661 ggatagatac gttcttttac catcggcaaa agatccgtaa caacttttct tcctttgtca
    69721 tctgcaacag cagaaataac tcctttaggt ttgtacaata aatagtaacc gtattcttct
    69781 tgataaattg ggacttcatc cacttccaca gtgtcatgtt tcccaacttt tatccctaat
    69841 tcccttacaa cttgtccgtt tacttttaca cgtcctgcaa ggatgaattc ttctgctttt
    69901 cgacgagaag tgatgccagc gtgggcaatt actttctgca atctctccat tacaattatt
    69961 ctcctttatt cattccatca aagaatagat ctggtgacac gtcttcttca agtgaaattt
    70021 ccatttcttg tgtatcaggc agttcttcta atgatttcaa accaaaataa tctaagaagt
    70081 agccagttgt tccatataat atcggccgac ctggtccttc tactctacct ttttcttcaa
    70141 tcagtttatg ggcagctaga cgctgtaccg ctcctgaaac ctgaactcct cgaatctgct
    70201 cgatttcagc tcttgtgatt ggctgtttat aagcgatgat tgctaaggtt tcgacggcgg
    70261 cttgagataa tgtgtttgat gtcccttgcg catatcgctt aagtaaggga gccagctctt
    70321 ttttagtcgt taatacataa tggctttccg tttcaaaaat atgcaaagct gagtgcgagt
    70381 tttgagtgta agattctttt aattctttca ctaaacttgt gacttctgct tcgctgagat
    70441 ttaacagtcc gcttaattct tcattactga tcccttcctc gccaacaata aaaagaaggg
    70501 cttcaagttc acttaatttc gtcatgttcg gtatctccta ctgctggata aagcataatt
    70561 gattcatagt tttctttttg ttgcgcaatg atctgccctt ttttcatcaa ttctagcaaa
    70621 gccataaaag tagtgacaac ttcactttta gaaggtgact caaaaaaaga atcgaaagta
    70681 cagccatgcg catttctttt tatacgctct tcgataaata ccattcgttc ttcgatcgtt
    70741 gtgtcatcgg gcgtgatcgt tttttccact ttttgagaaa atttcttacg ttctaagatt
    70801 ttctgcatgg cacggatcaa atcgattttt ttgacttgtc ctcgcttcaa cggcttttca
    70861 gcatccgcta acccatcgat gttcataggt tcttttgtgt aataaagact tcgttcctct
    70921 gctttttctg tcagtctttc agctgcatat ttatatttac ggtattctaa aagctgagtg
    70981 accaattctt ctcgaggatc ttcttcccat aattcttcat cgtctgtgac gaattcttgg
    71041 acaggaagca atgtcttgct tttgattgcc atcaatgtag cagccatcac taagtattct
    71101 cctgcaacag ctagctcgag tgttttcatt gtatggatat aattcatata ttgctccgtg
    71161 atcgtagcga tcggtatatc atatatatcg atttccattt gctgaatcag atgaagcagc
    71221 agatcgagag gaccctcgaa tacatctaat ttaatattga tctccgtcaa ctcattcact
    71281 ctttcttctt tttctttgct agccattttc caatgtaagg ctctgtgatt tcagttgcac
    71341 ggatggagac atccgggtac ttttcctgta attcatctaa catttgaaag cccatattct
    71401 caccgcgata agacggattc aaagaaatgt catggatgat cagctgatcg ttcgttgccc
    71461 agtaaatacc aataatccct tgaatgttgg ttgaaccttc tggcgtaaag aaaaaaagtt
    71521 cataatcgtc ttcagattcg tacgtatcaa tctccttcaa caaagaagtt ttacctgtca
    71581 gtttcttatg gaaactcaat agtcccatag caattttacg gttttctttc cggtaatgtg
    71641 ttaacatctt ctcaccttcc tatgactaag cccttggaaa atgctgctta taaacatccg
    71701 tcattctttt cttcgtgata tgtgtataaa tctgagtcgt agagatatcc gcgtgaccta
    71761 acaattcctg tactgtccgc aaatcagctc cattttctag cagatgtgtg gcaaagctat
    71821 gccgcagcgt atgtggtgtt acttctttgt aaatcccagc ctctcgtact aactgcttca
    71881 gatttttcca gattccttgc cgagaaagac ctttcccatg attattgaca aatacatgca
    71941 tttcagatgc gtcctttaca agtaatggac gtgcttcgtc taagtagcgt tccagccatt
    72001 ggatcgcata atcacctaaa ggaatgatcc gctctttatc tccttttcca actgtctgta
    72061 ccagtccaag agaaagatga agatctccca atttcaatcc aatcaattca ctgacgcgca
    72121 tcccagtagc atacatgact tccaagattg cacgatctct tatccctaac gtttttgtcg
    72181 tatcaggtgc ttcaatcagc ttttctactt ctgtcagagt caatgtactt ggtaatttct
    72241 gtgctttctt cggcgtatcg atatgttgca tcggatcatg atcagtgata cgctcttgcc
    72301 gtaaaaactg atggaatctt ctgagacttg agatcatccg agtaacagtt gctgtcgctt
    72361 ttttttcttc atgcaacttc tccagaaaac ggatgactaa aaagcgatcg acatcctgcc
    72421 atgaagcaat tttctgctcc tgtagaaaat gcaagtactg ttccaaatca cgttcatagc
    72481 tttgtcgtgt attggcggac aatccccgtt caatattcaa gtagtggaga taatcgttga
    72541 tttgctcttt cataataatc cccctcatgc ctctctatca tatcagatac cgcttcattc
    72601 tgatagtctt tttccataat ctgagcaaag aaaaagccgt ctagacactc agacggactg
    72661 gtttcttttt tctttttcat agcattcctg acagattcca tggaacgtta gccgatgatc
    72721 ttttactaaa aaatgataac gttcttctac tacttgctcc acttcaagta aaagatcctc
    72781 ttctacttcc tcgatcgtgc cgcagttcaa gcatagtaaa tgatgatgga aatgtttggc
    72841 gccttctttt ctcaaatcat aacgggctaa tccatcatta aaacttactt tatcgacaat
    72901 tttcaattct gtcaagattt caagggttcg ataaacagta gcaagtccaa tttccggact
    72961 cttttttttc actaaaaaga agatttcttc tgcagataaa tgctctttct cattttccaa
    73021 aagcacaagc agagtagctt ctctttgcgg tgtcagctta aaccccgctt catgtagctg
    73081 ctgttttgtt tttttcatcg ccgaaatagc attcatgacc atttactcaa ccccaacaat
    73141 ttataataat tacaaataaa aaaagatata acactatgtt aattaaaatt attataagct
    73201 agttaagaaa aacatgcaag ggattctcaa ttagattttt tgactagttt tgtcacaccg
    73261 ttttcttgtg taatcaatcc ttgtttcaaa agattaccta atgcgcgctt aaatgctcct
    73321 ttgctgatac caaaagcttt catgatttct tctggatccg atttatctgt aaagttgatt
    73381 tgctgatctg cagaacgttc taaaatggtt aaaatcattt gtgcatcatc tgatatagct
    73441 tcatatgctc ttggcttcaa tgaaagattc agtacaccgt ctggtcggac accaatcact
    73501 cgacctttta tctgctcgcc tagacgaggt tcattatatc gttcagaagg atggataaac
    73561 ccaagataga aatcttccgt taaaacgtac gtacctgtta atttcaagcg gtatgctgtt
    73621 cctatcacat ctttgttttt catttcttct gttccatatt tgcttaaaga tttgaatatt
    73681 ttttcatctg ctaaactgcc ccaaatacgg tcttttgcat caactctaag ggaaatcatc
    73741 aagcgatccc cttttttagg ccatagctct gtcattgtcg gcaattcatc aagagatacg
    73801 acaaagtctt tatctggaag accgatatcg acaaacgcac ctaaatcttt tcgtgtagca
    73861 gtaacgattc caaaagcgta atgaccgata cggcttttag gaatttgtgt agtgatccga
    73921 ttttcatgtt tttgattttg gtaaccgaat ccttcaactg cttcaccgat tttgtgttca
    73981 ccttctgatt tgcttaagcc aaaggtttgt ccgttttttt ggacaaagta gtttttttca
    74041 ttttcatcaa taataatacc tgtaaatata gttgctaata aatcgttcat ttttttctcc
    74101 ctctatagtg aaaggttttc ttaatacatt tctttgctat cataacataa tgttatctac
    74161 ttgaatagct gagccttggt ttggaaaggt gaaaaaagga gtatgacaac agttgtgtca
    74221 tactcccctt tttcgaataa acggtgttaa acggcaggct cacagcctct ttcatttgcc
    74281 taaaattaat tgctcccatt tcctccgaac catccagttt tacagttgat ggttacgatg
    74341 taaaatggca aagcactttc tccaatgcct ttcatcaatg cagattttgt tgaagtggca
    74401 tatagattat aatagccttc cccattttcg attctggctt tttctaatct gtaacctcct
    74461 tcaacgatat atccacgttg gatacatgta gcaatcactt tgctttggac acctggtgcc
    74521 cacgtccatg ctggattaga tgcatcggca atatcacttg ctctggcaga tttgattggc
    74581 tgattcgctg agtttgaaga ggaaggggtc gaggatgccg cttcttgctg tgctgcttgt
    74641 gcgaccgcag cttgttgagc ggcttgagct gcagctgctg cttgagcggc ttgttttgct
    74701 tgttcttcag ctgctttttt cgcttcttct gcttttttct tttcctcttc ttcttttttc
    74761 tctttatcag tgagcatctt atctacttgt ttcaattgtt cattcaactg agcagataga
    74821 tctgtatttt tcactttttc tactgcttct tttgccgtct tgtattggtc gcgtgttgct
    74881 gaatccacta cttttccgtc tttaaagact atagctgttt tttctttagc tgtttgcaat
    74941 tcagcatatt gatttttagc ttctgtttgt gcttgttgaa tcaattttcc aaaatctgtg
    75001 ttatccgttt cgattgcttg gatctcttta tctgctttca acttgatgtc atctgctaga
    75061 tgatcatcga cgatggcagg tgctatgaac cactcattca ctttttggat atctcggatc
    75121 ttagattgga tatcttggta agttgtttct aactctgaat atcctttttc attcttaaga
    75181 ctggataaat cattttttag ttttgataaa tcttgatgaa tcatatctgc acgaataaat
    75241 tgatggtgat catctgtata aaaggcagca agtttctcat gaagttcttc catttgttgc
    75301 tgtctttttg cttctgctgc tacttcggct tctacttttt gatgatggtc ataataagcc
    75361 cagcttcctc caccgagtag aagtactgct gctaaaacga gataaggtac tttttttgat
    75421 tttttctttt gcgctttatc tggatctgtt ttttctaagt ttgattcttg atgtgttttt
    75481 tcagtatctg gcacgattgt tattgatttc gcttctgctt caaacagctt aggctggttc
    75541 tcttcttttt ctatttttgc ttcaagatca gtaccctctt tgcttacaga ttgattttca
    75601 gcatcggatt cactatttat ttcatcgggc tctgttgact ttttgtctgt gttttcgagt
    75661 atttcttttt tctctgcttc cggcagttta ttcgtttttt ccttttctaa ttgtttttca
    75721 gtaaatactt cagttttttc tgtgatatcc gtttttacgt cttttatttt tactgtttct
    75781 ttacctgtgg cgttttctct atcagagtct ttttcagttt cttgttcctt ttctggctct
    75841 actttgtttt cctgtgcaat tttttcactg atcattttga gcagctcttt cgcgtgctct
    75901 tcttttgtat cttcacgatg tgctttgata taagcggata gtatcgaatt ttcttcctca
    75961 ttatcgacga tttctttcag ttcagctact cttctttttt gttttgattc aagcgtttct
    76021 tcttgattct ttcctgattc ttccgaagtc tcatgaacag ttgctttctt ctgttcattt
    76081 ttctctatat tagttacttg atcgtgagca ggtggttctt cgtcttctgg ttctgctttt
    76141 tcgaataatt ccattacaga ttctagaggt aaatctttca attccgacca ttcgattgga
    76201 tcgtttaatt ctgtatcagg aaagactgca tcttcagttg atttatcctg catagctgca
    76261 tctgtatttg tttcttcaat tgattctttt atttgctgat cttctgcagt tatttgatga
    76321 ccgcagtctg gacagatagt attctgctcc atttttttcg atccgcaatt tggacatttt
    76381 ttcatcactt agcgatcggc tcctttacta ggtttacgct accatttaat ctatcatatt
    76441 ctatacgtca cgccaatagc ttatttttag gatttactta aaaataatga
//
LOCUS       DANJMI010000007        72221 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000007 DANJMI010000000
VERSION     DANJMI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.007"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..1087
                     /locus_tag="PWU81_000576"
     CDS             <1..1087
                     /locus_tag="PWU81_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1109310.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="PWU81_000577"
     CDS             complement(1305..2447)
                     /locus_tag="PWU81_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1109311.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="PWU81_000578"
     CDS             complement(2452..2637)
                     /locus_tag="PWU81_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL1109312.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="PWU81_000579"
     CDS             complement(2991..3236)
                     /locus_tag="PWU81_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1109313.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="PWU81_000580"
     CDS             complement(3230..3631)
                     /locus_tag="PWU81_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL1109314.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="PWU81_000581"
     CDS             complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="PWU81_000581"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDL1109315.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5649..7415)
                     /locus_tag="PWU81_000582"
     CDS             complement(5649..7415)
                     /locus_tag="PWU81_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HDL1109316.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7428..8222)
                     /locus_tag="PWU81_000583"
     CDS             complement(7428..8222)
                     /locus_tag="PWU81_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL1109317.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8232..9608)
                     /locus_tag="PWU81_000584"
     CDS             complement(8232..9608)
                     /locus_tag="PWU81_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1109318.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(9728..10375)
                     /locus_tag="PWU81_000585"
     CDS             complement(9728..10375)
                     /locus_tag="PWU81_000585"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HDL1109319.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="PWU81_000586"
     CDS             complement(10459..11886)
                     /gene="uxaC"
                     /locus_tag="PWU81_000586"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL1109320.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="PWU81_000587"
     CDS             complement(11960..13039)
                     /gene="uxuA"
                     /locus_tag="PWU81_000587"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL1109321.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(13042..14673)
                     /locus_tag="PWU81_000588"
     CDS             complement(13042..14673)
                     /locus_tag="PWU81_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL1109322.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            14977..15648
                     /locus_tag="PWU81_000589"
     CDS             14977..15648
                     /locus_tag="PWU81_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1109323.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(16373..16708)
                     /locus_tag="PWU81_000590"
                     /pseudo
     CDS             complement(16373..16708)
                     /locus_tag="PWU81_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16805..17257)
                     /locus_tag="PWU81_000591"
     CDS             complement(16805..17257)
                     /locus_tag="PWU81_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109324.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(17264..17812)
                     /locus_tag="PWU81_000592"
     CDS             complement(17264..17812)
                     /locus_tag="PWU81_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL1109325.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(17857..18189)
                     /locus_tag="PWU81_000593"
     CDS             complement(17857..18189)
                     /locus_tag="PWU81_000593"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109326.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(18203..18460)
                     /locus_tag="PWU81_000594"
     CDS             complement(18203..18460)
                     /locus_tag="PWU81_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109327.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(18655..19197)
                     /locus_tag="PWU81_000595"
     CDS             complement(18655..19197)
                     /locus_tag="PWU81_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109328.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            complement(19218..20309)
                     /locus_tag="PWU81_000596"
     CDS             complement(19218..20309)
                     /locus_tag="PWU81_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL1109329.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(20317..22527)
                     /locus_tag="PWU81_000597"
     CDS             complement(20317..22527)
                     /locus_tag="PWU81_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109330.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(22543..25071)
                     /locus_tag="PWU81_000598"
     CDS             complement(22543..25071)
                     /locus_tag="PWU81_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL1109331.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(25123..25533)
                     /locus_tag="PWU81_000599"
     CDS             complement(25123..25533)
                     /locus_tag="PWU81_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109332.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(25546..25764)
                     /locus_tag="PWU81_000600"
     CDS             complement(25546..25764)
                     /locus_tag="PWU81_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109333.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(25761..26753)
                     /locus_tag="PWU81_000601"
     CDS             complement(25761..26753)
                     /locus_tag="PWU81_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL1109334.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(26832..27743)
                     /locus_tag="PWU81_000602"
     CDS             complement(26832..27743)
                     /locus_tag="PWU81_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL1109335.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVSAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(27762..28106)
                     /locus_tag="PWU81_000603"
     CDS             complement(27762..28106)
                     /locus_tag="PWU81_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109336.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(28126..28682)
                     /locus_tag="PWU81_000604"
                     /pseudo
     CDS             complement(28126..28682)
                     /locus_tag="PWU81_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28763..29263)
                     /locus_tag="PWU81_000605"
     CDS             complement(28763..29263)
                     /locus_tag="PWU81_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109337.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(29264..29809)
                     /locus_tag="PWU81_000606"
     CDS             complement(29264..29809)
                     /locus_tag="PWU81_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109338.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(29806..31062)
                     /locus_tag="PWU81_000607"
     CDS             complement(29806..31062)
                     /locus_tag="PWU81_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL1109339.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(31422..31733)
                     /locus_tag="PWU81_000608"
     CDS             complement(31422..31733)
                     /locus_tag="PWU81_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109340.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(31756..33066)
                     /locus_tag="PWU81_000609"
     CDS             complement(31756..33066)
                     /locus_tag="PWU81_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDL1109341.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(33680..34132)
                     /locus_tag="PWU81_000610"
     CDS             complement(33680..34132)
                     /locus_tag="PWU81_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109342.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(34134..34499)
                     /locus_tag="PWU81_000611"
     CDS             complement(34134..34499)
                     /locus_tag="PWU81_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109343.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(34598..38881)
                     /locus_tag="PWU81_000612"
     CDS             complement(34598..38881)
                     /locus_tag="PWU81_000612"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HDL1109344.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(39028..39267)
                     /locus_tag="PWU81_000613"
     CDS             complement(39028..39267)
                     /locus_tag="PWU81_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109345.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(39357..39629)
                     /locus_tag="PWU81_000614"
     CDS             complement(39357..39629)
                     /locus_tag="PWU81_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109346.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(39712..39963)
                     /locus_tag="PWU81_000615"
     CDS             complement(39712..39963)
                     /locus_tag="PWU81_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109347.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            complement(40959..41870)
                     /locus_tag="PWU81_000616"
     CDS             complement(40959..41870)
                     /locus_tag="PWU81_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1109348.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            complement(42066..43901)
                     /gene="lepA"
                     /locus_tag="PWU81_000617"
     CDS             complement(42066..43901)
                     /gene="lepA"
                     /locus_tag="PWU81_000617"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL1109349.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            44048..45268
                     /locus_tag="PWU81_000618"
     CDS             44048..45268
                     /locus_tag="PWU81_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1109350.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            45288..45782
                     /locus_tag="PWU81_000619"
     CDS             45288..45782
                     /locus_tag="PWU81_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL1109351.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            45760..46446
                     /locus_tag="PWU81_000620"
     CDS             45760..46446
                     /locus_tag="PWU81_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109352.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(46597..49206)
                     /gene="clpB"
                     /locus_tag="PWU81_000621"
     CDS             complement(46597..49206)
                     /gene="clpB"
                     /locus_tag="PWU81_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL1109353.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(49457..50503)
                     /locus_tag="PWU81_000622"
     CDS             complement(49457..50503)
                     /locus_tag="PWU81_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDL1109354.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(50514..50786)
                     /locus_tag="PWU81_000623"
     CDS             complement(50514..50786)
                     /locus_tag="PWU81_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL1109355.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(50821..51729)
                     /locus_tag="PWU81_000624"
     CDS             complement(50821..51729)
                     /locus_tag="PWU81_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDL1109356.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(51861..53093)
                     /gene="pepT"
                     /locus_tag="PWU81_000625"
     CDS             complement(51861..53093)
                     /gene="pepT"
                     /locus_tag="PWU81_000625"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL1109357.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(53108..54229)
                     /locus_tag="PWU81_000626"
     CDS             complement(53108..54229)
                     /locus_tag="PWU81_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL1109358.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(54226..54927)
                     /locus_tag="PWU81_000627"
     CDS             complement(54226..54927)
                     /locus_tag="PWU81_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDL1109359.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            complement(55071..55511)
                     /locus_tag="PWU81_000628"
     CDS             complement(55071..55511)
                     /locus_tag="PWU81_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL1109360.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            complement(55640..56566)
                     /gene="dnaI"
                     /locus_tag="PWU81_000629"
     CDS             complement(55640..56566)
                     /gene="dnaI"
                     /locus_tag="PWU81_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDL1109361.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            complement(56566..57939)
                     /locus_tag="PWU81_000630"
     CDS             complement(56566..57939)
                     /locus_tag="PWU81_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL1109362.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            complement(57962..58453)
                     /gene="nrdR"
                     /locus_tag="PWU81_000631"
     CDS             complement(57962..58453)
                     /gene="nrdR"
                     /locus_tag="PWU81_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDL1109363.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            complement(58765..59097)
                     /locus_tag="PWU81_000632"
     CDS             complement(58765..59097)
                     /locus_tag="PWU81_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL1109364.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            complement(59149..59778)
                     /locus_tag="PWU81_000633"
     CDS             complement(59149..59778)
                     /locus_tag="PWU81_000633"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL1109365.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            complement(59742..60578)
                     /gene="mutM"
                     /locus_tag="PWU81_000634"
     CDS             complement(59742..60578)
                     /gene="mutM"
                     /locus_tag="PWU81_000634"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HDL1109366.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            complement(60623..63268)
                     /gene="polA"
                     /locus_tag="PWU81_000635"
     CDS             complement(60623..63268)
                     /gene="polA"
                     /locus_tag="PWU81_000635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL1109367.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            complement(63394..64077)
                     /locus_tag="PWU81_000636"
     CDS             complement(63394..64077)
                     /locus_tag="PWU81_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDL1109368.1"
                     /translation="MNNPSTLMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            complement(64070..64555)
                     /locus_tag="PWU81_000637"
     CDS             complement(64070..64555)
                     /locus_tag="PWU81_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDL1109369.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            complement(64862..66196)
                     /locus_tag="PWU81_000638"
     CDS             complement(64862..66196)
                     /locus_tag="PWU81_000638"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL1109370.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            complement(66327..67088)
                     /locus_tag="PWU81_000639"
     CDS             complement(66327..67088)
                     /locus_tag="PWU81_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL1109371.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            complement(67104..67586)
                     /locus_tag="PWU81_000640"
     CDS             complement(67104..67586)
                     /locus_tag="PWU81_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDL1109372.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            complement(67606..69786)
                     /locus_tag="PWU81_000641"
     CDS             complement(67606..69786)
                     /locus_tag="PWU81_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL1109373.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            complement(69930..70661)
                     /locus_tag="PWU81_000642"
     CDS             complement(69930..70661)
                     /locus_tag="PWU81_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDL1109374.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            complement(70730..71671)
                     /locus_tag="PWU81_000643"
     CDS             complement(70730..71671)
                     /locus_tag="PWU81_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDL1109375.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            complement(71851..72219)
                     /locus_tag="PWU81_000644"
     CDS             complement(71851..72219)
                     /locus_tag="PWU81_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL1109376.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT        21128 a        15163 c        11245 g        24685 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gctaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcatt ctttaaacag aaaatctttc tataaaaaac aaaaaaaaac gattaggcta
     3781 ggctaatcgt tttttgaaca ttcttaaaaa atccaaatta ttttttataa ctgaaattgg
     3841 gtattgatga ccatcgtttc tgtagatagt aagcaatcat tttaggtttt ctatctctag
     3901 taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgacttgtt gcaaaatctg
     3961 caaaattcca cacttgttca ccaacaaaat tttcacatgt atctattact tcgtgatttg
     4021 cttcgtaata agccacttga tactcttctg taaacattgt tggtactgta tcatgaaatc
     4081 cagctactgt atctgctcca tattctgtaa acattattgg cgtatcagga catctttcca
     4141 tccatccatc tagctctttt ttcaacagat attttgcatg tgccagatcc cctcccgaaa
     4201 cataccaacc ataataacga tttaaacata aaacatcgac aatatctcct accttacaaa
     4261 catctggcgt tgattttaga taagtaacga ttgttacagg tcttttctgc ggatcaagtt
     4321 ctttagttaa ttcaactaaa ggttcaaaat attctttagc accttcggaa tccgaatctg
     4381 gttcatttgc aatagaccac attacaacac ttgggtggtt cttgtcgcgt tctatcaatt
     4441 ccttgatcac cgttttatga gaattttctg ttcgtatctc tttccacgta ttatgttttt
     4501 ccacattatc atctaataaa gtggccatga aatttaaatg taaccctact gcaggtactt
     4561 catcaataac tacaatacct tctcgatctg ctaatcgcat aatttcttct gagtaaggat
     4621 aatgtgatgt tctaaaagaa ttagcaccta tccatttcat taaattaaaa tctttaatat
     4681 ttacagcctc attcaaacca cgtccattaa catacgaatc ctcatgctta ccaaatcctt
     4741 taaaataaaa aggtttatta ttaatcaaaa atttgccatt ggtaacctga acactgcgaa
     4801 taccaaattc ttctgtatag gtatcgatca ctttattatc atcaattaaa ctaattttca
     4861 tactatataa ataagcgtct agaggttccc aaaaaattgg attcttaagc tgaatcattc
     4921 ctttctttcc ttcagcttca gctacaagtt gagcctcttc gtcatatatc gctattaaga
     4981 tttttccggt ccattctgta tcgatattga attctacact tgcatccaaa tcagttattc
     5041 ttgtattaat agtaatatca cttatatagt ctttcggagt tgtatataac ttaacaggtc
     5101 tatgaatacc tgcataattg aagaaatcaa aattaactaa gttttccttt tttatatccc
     5161 cattttcctg tactgtttcc ttcataatcc ccacaggtaa agtactatcg tcaataatat
     5221 tgtttacagc aactgtaatt cgattattcc cgtcttttac aaaccgatta atttcagact
     5281 caaatggtgt aaacccacct ttgtgttgag taataaactt tccattcaaa aaaacttttg
     5341 cttcatgtgt tgcagatcca aaacgaagaa ctaatctttc ttctaataat cttttaggaa
     5401 gagtcacttc tttttcatac cacacccatc caacatgatt tctaatgttc tctgtttcaa
     5461 ataaatcatt ataagaagag ggaactgaaa tcggataact ctctttttca tttattggtt
     5521 ctaaagcaag ttcttcctgt aagtcatcat ttgtatctaa tttaaatctc cagagaccgc
     5581 ttaagtcaat tacttgcctc gtctctgtca taatgggata caacatataa accactccta
     5641 tctaaatatt aaataaatgt atcctttaaa ccacaataag catctattgg acttactccc
     5701 ttaaattcag aaagacccat tagtttgtcc accaatattt ctaaagtttc ttgttttcca
     5761 tcataagtat taatataagt ctttacttga ggtacatcag ccaaatgaaa cggacactgt
     5821 acagatacaa aaattgttgg aatttcatga atgtaaaacg gtatatccgg agttccttta
     5881 cttgctggcc agacaattcg ttgaactgtg cctggattaa cattagctat attaatgatc
     5941 aagtcatatt ttttagttaa atttttaatt ggtctcttag cagcatatat tccagctata
     6001 tttttaattt tttcttcttg cgataatcct ttcaattctt cttcaggtga aatgtatttt
     6061 tcaacttcaa atccttttgc tgacaaaatc tgtttcaata aatcaatggc ttgaggtcta
     6121 tttccagcaa ctacttcacc aaacccacct tttaccccat gtacatctac tagtaaaatc
     6181 cttttatatt tttctggtac aataggccaa atatcttgtt tatctttaac taatgtaatt
     6241 gcttggtcag ccacctcttt aaaaattgat ttattttcaa ttaaaccaat tttttccaga
     6301 gcttcttgct taggtaataa aatttgttct tttgcttttt tgtgtaatcc caatttagct
     6361 tttaacccta aaattctttt taaggcatct aataaccgat catcagtgat aattccttta
     6421 cgatagcctt ccatcatata attaaagtct tcatctggat cattaaaaaa tagaaataga
     6481 tcacatccag ctgcaataga agtgggcaat aattctgatc gtttcattgc cccagtcatt
     6541 cctaacatat gactagcatc agtaactact aatccattaa atccgagttt tcctctaagt
     6601 aagtcagtga ttaattcttt tgataaagtt gcaggaagta aatcttcatc tttcagatca
     6661 ggattaaaat gttttgaata agctggcata tggatatgac ctgccataat tgaaggtaat
     6721 ccagcttcta ttaagcctga gtatactttt ccaaaagaat tatcccattc ttccgtacct
     6781 aaactattaa tactaaaaga tagatgctga tctcgttcgt caataccgtc accagggaaa
     6841 tgtttagctg caggcaaaat tccactctcc tgaatacctt tcatataagc taaactaagt
     6901 tctaaaactt tatcagggtt ttgtccaaaa gatcgactag aaatgatagg atttctccaa
     6961 tttctattaa tatctacaat aggagcaaaa ctccagttac atccaatagc cgatgcttca
     7021 atcccagaaa ctctccctaa ttcataagca tatttcgcat catttgttgc accaattttt
     7081 acttctacac caacttcagt accatctgta caagctccgt taccacctgc ttccgtatta
     7141 gcagcaatta atagaggaat tttactttct gtttgtaaga tatagttttg atcatatacc
     7201 tcatcagcag ttcctggatt atatcgtaca gctccaatat ggtactgatt gaccatatta
     7261 ctcaagtaat ttttatcacg actagatccc atattaacaa acaattgacc tattttttct
     7321 tccaaagtca tagaatcaat cgtctcttca atccaagaaa tagcatcttc atctaaatta
     7381 tatggttttt ttcgtaaatc tacttttata gtcattttct ttctcctcta cttattagta
     7441 agatttttcc aaaaagtatc tatatatttt tgttcaatat ctacataatc tcccgataaa
     7501 catttaaata aaacatcagt tcttaagttt tcccatgatt ctttttcttt attgaccaaa
     7561 ataggataaa agaaaacttt atttttattt gcagcttcta gatcaccagg agaatcacca
     7621 atcattaata tcttatccaa atcacaacca ttttcaatta cacttcgaat aatatcttct
     7681 ttttttcctt tatcttgaca acacagttca tcaactaatg ataataattc atgtctttcc
     7741 cattcttctt gaacagcttc cttattagct gagcttacaa taattatctt cccatatttt
     7801 ttcagagctt ttatagtatc tctagcactt aaaaagggtt tatctaatcc ttttgcttga
     7861 ctaatctgtt gattgacttc ttccgaccat tttaaagctt ttaataaatc ctctgactga
     7921 gaatattcta tttccctctt caaagatgga ttagacaatt cttttgttag agatacccag
     7981 tgctttaatc gattaatcga tgttacatca tttttttcta atgttttaac taaacctaaa
     8041 aatctgttta ttccacgagt ctctgaatat aaattaatat tattccaatc ctgtaggaat
     8101 ttttctctat gatgaacatc aaaaatctct gcagcaattg gtccgaaaaa gagattgtgt
     8161 ttataagtca tcgtatccat aacacagcca tctgaatcaa tacaaaatat ccaattattc
     8221 atatttctct cctatttatg tttttgtttt aaaagctcgc aattttctaa gactttatct
     8281 ttagataatg gataaaggaa aaataaaatt aatcctacaa tcagaaaaca aattgatgga
     8341 attaatgttg aagcgttata taacttttct aaaacatctc ccgtttgtac atttgctaaa
     8401 gagtcatagc ctataataga aagagtccaa cctccgacgc ctccagctag agcttgacct
     8461 atttttctag caaaagaata taccgcatat acggtcccat cttctctttg atcagacttt
     8521 acttcttgat catcaataac atcagtaata tttgcccaaa taacagtatt aaacacaccc
     8581 aaacccatat agccaaccga actaagaatt aagaatacat acatattact agcttttatc
     8641 aaaaataaga tcaaatatac tattgctgaa aagaacattc ctactgatgc cagttctttt
     8701 tttccatatt ttttacttaa aaacaaagtt aaaggcgaaa caactattaa aaccaaaata
     8761 ggattgataa aattcattaa aactattccc tgtgcatcat tatagtaatc aggaaataca
     8821 taattattca tactcataat taataattga gcaaggagta agaaaatagc cgcaccaatt
     8881 atagaaagta atgccctatt tttaactaac ccttttaaag attttaacaa tgaaactttg
     8941 tattgagatt ggtcaattct tactcgttca gtagttagtt tataacatat taaataacaa
     9001 ataagtgcac atatcgaaaa tattcccgca acaattgtaa aagttgatcc accacgaact
     9061 atttgagcgc ctgaatcatc tcttgtataa ataaataatg gtgttaatac accaatgacc
     9121 aaacttgcta aagtagctcc aacatttcgg aatgtcgata gcattgttct ttcttctgta
     9181 atagaagtaa tagctgatgc cattgatcca taaggaatat ttatagatgt gtaaaaaata
     9241 ctcccccata atatatacgt aacatacatg tatactacct taacgctcat agaagctgaa
     9301 atcatagaag tttgatacat taaaaaactt gctaaagcga ctggaccact cattcttaaa
     9361 atccacggtt taaacttacc ctgcttagtt gctttcgatc tatcaacaat acgccccata
     9421 gttacatcag taactgcatc gataaatctt gctactaaaa ataatgttcc aacggcagct
     9481 ccgcttatcc ccaatacttt tgtataaaag atcattaaaa aagaactagc aaaaatgaaa
     9541 gtaaaatcgt ttccaaaatc tccaaacata tatcctattt tgtctcttat accaaatttt
     9601 ctttccatac aattctctcc cttctgttgt taagcgtttt cattctgatg aaagtacgta
     9661 ctatactctg aagcgtaaag agtcagtatt tattttttac atttcagagt attatttgtt
     9721 ttttctccta ataattcttt atctcataaa acttatctat atatttacta gctactcgac
     9781 ttacttcttc aaaatcacct ttttcaacag aagccaataa attcccacct acaccaactg
     9841 caacaactcc tacttcaaac caatcttcta agttctctat acttactccg ccagtaggca
     9901 ttatatttac gtgtggtaat ggagcttgat aggcttttac aatggatgga ccaaaagcag
     9961 aacctgggaa tagtttaatg atatctacgc cagcagtaag tgcctccttt atctcagtaa
    10021 ttgtcataca acctggtaaa taaggaattt gatataaatt acaaatctca gctgtttgtc
    10081 gatcaaaaga tggactaacg atatattctg ccccagccat aattgcgata cgagcagtaa
    10141 acgcatctaa aaccgtaccg gctccgatta ttacatcttt ttgatcagca tattttttag
    10201 caatttcttt gattatttct tcagcattag gaactgtaaa agttagttca atccctttaa
    10261 ttccacccgc aataattgaa tttattgctt taatggcctc atccttattt ttcccacgta
    10321 caaccgcaat gactccagcc gcttctaact tacttaaaat ttctactttt ttcatatcta
    10381 tctccttcct ttatggatac atactttgtc tagctgtact aacttacaaa gaataaattt
    10441 aagcttttat ttcaagtatt attttctact tccgatttca aaaaaattaa aataattata
    10501 cgcattacga taacaaatat tctccactat ctgactaaga tacatttcat catctggaat
    10561 tcggcctgct ttaacccact cgcttagtaa ttgacataat actcgacgaa aatactcatg
    10621 gcgaggatag cttagaaaac ttcgagaatc agtcaacata cctgtaaaat tcgacaacaa
    10681 actttgttgt gccatcgtag tcaactgttg acgcattcct tcagctgtat cattgaacca
    10741 ccaagcacat cccaattgga ttttttgaat cataccccct tggaagcttt gcattgctgt
    10801 tgctagtgga agccaatcag ttggatttaa tgaatataaa attgtttttg gtaagcaccc
    10861 actcttttca gcggtatcga acaatgctac aacattttgt gtcaaactag cttgatcccc
    10921 catcgaatca ccaccgacat ctactccaat ttgttccttt aatatacttg aattatttct
    10981 taaagcattt aaatgcattt gcatcgtcca accatatctt gtatactcag acatcaaatg
    11041 taattgaata cctgtaacaa attgtgcaat ttctgataaa tggaaatccg tttttcctgc
    11101 taaagctttt attaacagat tgtcgagtat tttttcatct gctatttcat aaaaataagt
    11161 attcaaaccg tgatctgcta atcgacagcc aatttgatga aaatgatcca ctcgttggct
    11221 caatgcttgc ttcaacccat taaagtcttt gattggtata gccgctgttt gactaagttt
    11281 taaaatatag ttcgaaaagc ctgatgattt gatactccag accttatctg gacggaaagt
    11341 aggtaatacc ttgaaagaat tatcctgttt agaaagctca gtatgatacc acaagtcatc
    11401 tgtaggatca tccgttgtac agatggcttt cacatccatc atctccaaca attctttcgg
    11461 gcgataggaa tccgtttgaa ttttttcgtt agcacgtcgc caaatttctg gtgcattctt
    11521 ctcagaaatt gtcaaatcaa tatcaaagta acgttgtaat tccaaatgag accattcaaa
    11581 aataggatta cctggcgcct tctccactgt ttttacaaaa gctaaaaatt tttcataatc
    11641 atcacctgga ccactgatca actcttcttc tataccatta gctcgcatca atcgccattt
    11701 ataatgatct ccagctcctt tatcatttaa ccatacctga gtcaaattct tgtattgctt
    11761 attttctagc aaatatgctg ggtctaaatg acaatgatag tcgataattg gttggtgttt
    11821 tgcaaattta tgaaaaagtc ttttagctat ttcattctct aataaaaagt tcttatctaa
    11881 aaacatattt aaacactcct ttccttccgt tagtttttag aagagaaaat ttttcctcta
    11941 aataaactcc cttatataat tatttactcg cccccttttc aagcgcttcc caaataccat
    12001 ttaaataaac tgcacctaga gctcggtcat ataatccata accaggtcta ccggtttctc
    12061 cccaaatcat ccgaccatga tctggacgaa tatagccatt aaatccatta tcgtacattg
    12121 tttttaatat cttatacata tctagagaac catattctga tttatgcggc gcttcgtaaa
    12181 aatcgagtcc attgtcactt aaatatttga tattacgaac atgtgcaaaa ggaatccgat
    12241 cccgttgaca aaattcagat agaatttcat agacatcatt atctggtcgt tctgcaatac
    12301 ttccagtaca taatgtaata ccattagaag gagaatccac tacattacac aaccaatcta
    12361 aatcgtcacg atttttaaca atacgaggta atccgaaaat ggaatatggt ggatcatctg
    12421 ggtgaactgc cattttaatc ccaacttctt cacatgtcgg gataattgct ttgagaaaat
    12481 atgcaaagtt ctctcgtaat ttctcttcat caacttcttt ataggcttct aataattgct
    12541 taatttgtga taaccgatga ggttcccaac ccggcaattc aaaattatta gtagaatttt
    12601 ctacttctct aataatttcc tccggatcag tagggatatt ttttgaaata taggctaacg
    12661 tagaagaccc atcatctaaa cgataatcca aatccgattt tacccaatca aatactggca
    12721 taaaattata acagataacc tcaatcccaa attctgccaa attgcgaatc gtctgtttat
    12781 aattctcaat gtattgatct cgtgttggta aaccaatttt gatatcatca tgaacattaa
    12841 cactctcaat aactttcaag gtaagtccat tggcttcgac ttcatctttt aaagatttaa
    12901 ttcgttctct cggccaaacc tctccggctg gtatatccat taatgttcca acaattcctt
    12961 tcattgctgg gatctgacga atttcttgta gtgagatttt ttcttcattt gccccatacc
    13021 atctaaaagt catttccata gttactttcc tccaacttct gttatatatt tattgattgt
    13081 tttacgaact gcttgaggac ctactagcat ttcgcaaaag attttttcag ttttctttcc
    13141 taccccaact tgatataaat ctaaaccaaa gatttcttta ttttctaaaa tttcctgtac
    13201 cgcacaatga atctttgaag gatcttgttc acctaatttc aatactgata atttatcctg
    13261 caactctacc aataacggat caggacttgg cacaaaattt tccccttgat catcgactgc
    13321 cagtaaataa cgtaaccaag cagcaatagc gataggaata aatgttagat tttctgcatt
    13381 tccgtctttt tcgacatact ttttaattgt ttcaccatag cgaatcgcta ctttttgaga
    13441 agtatctgtt gcaattcgtt gtggtgtatc tggaatatag ggattaggca aacgtttatt
    13501 aattacttca tcaataaatt gttttggatc aataatgcct ggattttcaa ctactggtaa
    13561 accttctaaa taacctattc ctttgattaa accaaccaaa tcatcatcct gcatctctgc
    13621 tgaaattgat tgatatccta gcagacaacc tgttactgcc aaagcagtat gaagtggatt
    13681 caagcttgtc gtaaccttca tgatgtctgc tttatcaacc gtctctctat ctgtcataat
    13741 cacaccagcc ttttccaatt taggccggcc atttggaaaa ctatcttcaa taactaaata
    13801 atgaactacc tcagtattag tgaaagcagc aatattggta tgtttttttg tgtgaatgat
    13861 atctaaatcc tcgagcccca tcatcaatag atcttccttt acattctctg aaggatttgg
    13921 cgtaattcga tcaatcatac tccaaggaaa agtcactttt tctttatcct ttaaataagc
    13981 aataaatttt tcagaaacat atccttcttt ttgccaagct tctgcaatcg taaaaatact
    14041 atcttgaaaa cgttgaccat tctgtgagaa attatcagta gaaaccatcg caatcggcaa
    14101 ctctccagca ttaaatctgc tatataacaa ggatgttaca atactcatcg tatgttgagg
    14161 attgctaggt ccagatttga tatcagcttt gactaccgaa aagaattgcc ctttagaatc
    14221 ttttaaactg taaccttttt cagtaatagt aaacgttgta aactgtaatt taggatcttc
    14281 aaaatattta accactcttt gatagtccgc tatattcttc ggattgcaat ataaactatc
    14341 agccgttact gccaaaagtc tttttttgag ttcaccatct ccactcataa caatttccaa
    14401 aatgtcattg ttatagtcat gataagcttt ttccactact tcttcatcaa atgtttcaca
    14461 cacaactact cctgtttcta attcattttg atctgctaag cattgagcaa tttctgcatg
    14521 aaatcctcga taaagatttc ctccaccaaa atgaagccat actggatgtt gctggccttc
    14581 ttgcttaact tctttgacat caaaacacgg cagtagaatc tgcatttcat tgaattcttc
    14641 gatagaattt tgccaatttt taatcgaaac cataattaac ctcctcaaac tgatatgtta
    14701 gttttgatac attttatatt ttaaaaccca ttgtaaccgt tgtcaatata aaaagggaat
    14761 aaacttgctt ttttctacca tatcagaatg attgtatttt ttattcagaa aaataaaata
    14821 ctccttcttt ttggactgat tttatcgaaa cttatttttt ctttttttca tgtattcttt
    14881 tatttttata actagtgtat tacttcaaaa tttgatatta tatggcatga acagaattaa
    14941 aatatcagtt aaaataaaag ttaaggagca ttgtgtatga caattaaaac tagtctccaa
    15001 aatcaagctt accaatctat tcgccgacaa attatctatg ctgacttaga acctggaaaa
    15061 aaagtatcag aaaaaaaatt agaagaaaca ctttctattg gtcgtacacc aatacgtgaa
    15121 gcactgattc aactccgaca acaaggtctt gttgatacaa ttcctcaaag cggaaattat
    15181 atctcattaa ttgatttaga ttcggccaaa aatgctcgat ttgtcagaga acatttggaa
    15241 cgacaaatta tgttagagtg ttgtgccaaa atgaataacc aaaaaaaacg gattttagaa
    15301 actataattt cagaacaaga aaaagctgtt agtcaaaaaa atgctcgtag tttttttcat
    15361 aatgataatt tattccataa agcttgtttt gaaattgcag agcgagaaga agtatggcaa
    15421 tggttagaaa ataacaatac acacttagaa cgatttcgat ggctacgagt aaaaacggaa
    15481 ggattaaagt gggaaaaaat tatggaacaa cattatcaac tatttgaggc attaacaaaa
    15541 caagatccag aagaagctaa ctttctaaca tcgttgcatc ttcatttaat gcttaatgag
    15601 caaaatattg ttttagctac tcatccaaaa tatttcaaaa atatataact tttaactctt
    15661 tttaaattaa atggttgttc tgatcagaaa aatatcgctt gttttcttat tatgtaatat
    15721 atttcataaa ctattctgca taacaactat tgtttgtact ttttacattg gataactaat
    15781 tatgactgct gataactttt gatattaaac tagtatctcc aatacaaata aaaaatagat
    15841 atcttgttca tcaaaattag ttcgtggata cttaccttca tttacatcat tcagcggttt
    15901 cccattttct tttattctat tatttcattt tctaatagac ttggcttcat ttcgcaatac
    15961 tttttttcaa taggcatcaa attggtgttg tttagatatt tagttgtagt tttgtgacat
    16021 gataagtatg ctcctttttc tcttttccac taatacaacg atcaaagggt gcttttttca
    16081 aaaaactaga aaaatcaaaa taagattcgt attttgattc tatatatcct gtttcaacta
    16141 catcttatat ttcacaatag aataaatagc cttttgcata gctgcttaac ttataagaaa
    16201 ttgcaattta cttcttcaaa gtgaaaacga atatgagtaa aataaaatta ttcttcttac
    16261 agataattaa aaatagcgcg taacaaaaat cggcattcaa tcacataatt aggattgatg
    16321 ccgattactt atattctacg tatagtttta gtattcttat tttttatcac cagtaaccaa
    16381 ttgagaacag ccctaaagca gaatagtctt ttatctcaaa acgcttaatt tcaggcttag
    16441 gaatatgaat aatcttttga gtatattctt tctcttttaa tcttactgaa gaaataccac
    16501 tgcctatctt ttttacaaat atttcatttg gtgtacagac taataaaaca atatttgtaa
    16561 tcaatccttg cactaaagaa ttaaaaggaa gactatttaa aacagggtgt gcataactat
    16621 aacatgtgaa gtagttattt tgaactttaa cttttaaaaa ctttaaaaat aatcttattt
    16681 gttcttctaa acaatcatct atactcatat aatcactcca ctaattaatc tccccaaaaa
    16741 gacacagcat taagagatat accaacacaa cagtgcaaat aactctacat cttccttcgc
    16801 aaccttattt caataaacca taaaaattat tttttaatag aaactgaaaa ttaaaactag
    16861 aaaaagtagt tttaccgaat gtaagagcat catcaccatc aatatagaaa tacattcttt
    16921 tccagttaca tttaattttt aaacaaaatt ctccccaaaa aggaactttt tctagtgaaa
    16981 acttggtaat ctctttgtaa ttaattcttg ttaaagaata attaaacact tcatttttac
    17041 ttgttatttt ttcattgact ataactagtt catctggtaa aaaaattaac caacaaacat
    17101 cgacaaacag taaatgatat aatccatttc cacctccaag catattcaat actggtgaac
    17161 atgccttaga aacacgaata tagttatggt ttaaatagat attttcttta aacattgctt
    17221 gtttaaaagc catatgctct tgttctaaac ttttcataaa cttttactct aatgcacagc
    17281 tagcagcagc tgctgcaatt cctgctacaa tatttacctt agctaactct ttagctgcag
    17341 cttttcctaa ttgtttttct gcaattttta ataataaatc cgcagctttt tcaaaattaa
    17401 aagcttttaa tgcttcaata aaatctccct gcgtacctat agcccaagcc actcctcctc
    17461 caggaagaga attaatggct acacatccag catatcgttt ccaatcaact ttctccattc
    17521 taggaacaat aatatgttca taaaagactc ttgctgctct tccttgttcg ttatcttgac
    17581 tgattcgttc acgaatagca ccaaaatcct ttatatggta tacaccatgt tcgtctaaaa
    17641 aaccaactgt atcaaaataa aaagttaatt catctactaa tttattcttt atttctaaat
    17701 caactgtatt cactttttct gtggcataca cacttgttcc agtactaaca aaagtactta
    17761 aacctaataa gaaaacacct aaaacaccaa atattttaat tattcttttc ataatttctc
    17821 ctccataaaa aataagttac tttttaaata tcttgtttat gaattcattc cccaaaggcc
    17881 ttttaagatt gatacagcag tccgttcact tcttttagac ttgtcataca agtcttttaa
    17941 atgatcccat atcagactag tttgtctatc tagcttcaat ccattaaacg ctaatgaacg
    18001 gttcattttt tctattactt tagataaagc tacagaaatt tttatacctt cacaacgcgc
    18061 atcttggatt tcaataatag tatcatcaat tagtttttga atttcttgcc tatcaacata
    18121 ttcaatctta gaaagagaat cttttaaagc tactaatgat tcaacgattt cttttttctc
    18181 ttcagacatt ttcttcctcc ttctagtgac ccgtaaagcc atgtagagca gagtccattg
    18241 aaccagtaat tacactatct acaacgccat tccaatttac tctacatttt ccatttttca
    18301 gatttgtcgg tccatacgga tagccttttt gacattcaat gttcggatta taccagatag
    18361 gattatacca ttcagccgca gaagcagatt cagtcgatac aaacaatccc acaccaacaa
    18421 atccaataca catcacacta tagccaatta gttttttcat ctcgtatcca tctcctaaaa
    18481 aatagaatag ttcttatagt tattcataag acatattttt tccgtttttg ttttatcctt
    18541 tctgtcctat aacaactttt ttcaagtaaa gaatgacata gtttttctta aaaaaacagt
    18601 ctgttttttt ctatttcttc ggtgtataat aaatatgagt gttcattttt tattttaata
    18661 cgcactccgt acataataaa tcttgatcgc atcaatcttt tgagcttctt gacctacttt
    18721 caattgaagt aatacaaatt gagtaacctt attcattgtt tcttctccaa ataacaaata
    18781 ttgatccgga ttctctatat cttgtgtcac ctggtaaagt ttcccagttg ttttcatttg
    18841 tacatgcggg tctacatcaa tactattgtc tttaatacaa cgatctgtca tatagtccct
    18901 cacagcttga ttgcgtttat aaacatctgt gaaattactc cattttctag caaaatcttt
    18961 taagaaattt tttgtcattt catctatttg catttgttgc gctgtctcgt cttctaatga
    19021 accaatttga ctgttacttt cagtactttg aagagttcca cttgtttctt ttactgcttc
    19081 gattgtttct tctgtcgttc tcaattgtgt tttttgtttt tcagaagaaa accaatgaca
    19141 accagtaaga cctatcaaac ataaccaact aaatactaaa attatccctt ttttcacgtt
    19201 tctagtcact gcctttctta tttcacctta cgaaccagac tacgaatttt tgtttgattg
    19261 tacgtgcgtt gataccgagc cataatccgt ccttgctcgg cattctgttc cacactagag
    19321 aaatgttgtc cgccatatga aacatctgtt ataattccgg tgtgaccata gattccagga
    19381 gacaatgcac cacctgcttg ccaattcacc acatctcctg cttgtaaatc acttggtttt
    19441 gggttcatga ttacttgcca accataggtt gtccagttat aatcttcccc tattaacatc
    19501 gcaaacatgt gtccagaacc cattaactgt gggccaccta attgagatac ataccatgcc
    19561 gtaccaccat aacattcccc attatttacg gatcgtccaa tgatccgatc caaaaccttc
    19621 gcattgcctt tactttttcc tgcaataagc gaagtttttt ttgttggtag gctattccaa
    19681 tgcttgatcg caaaagtatc atattccgtt aagttatagg acgttatgat actcattgct
    19741 cctcgataat acggactccc ttcggtaata cctgctgccg catagcctcc acgacctaga
    19801 atcaataaac aacttccagg atctttattg cccacacatt gattaaagtt gctttggctc
    19861 gccaccataa agccataata gtcataacaa gcttttaagt tcggaaactt catatagaaa
    19921 ccaccttcag aaccaccaat tccacgtgca gaaccttgtg ggtagggaca accaggaaac
    19981 caggtaatcc caaaaaagtt cgtatcgttt cttccagatt gtgactgtcc ataagctgat
    20041 tcatagccta attgagaaag aatcacactc ggtaataact gatactttac agctgcacca
    20101 actgctgctt gcgcatgttc atctgtgatt ggaccacgtc ctttaatctc tgaaaaaatc
    20161 ggtaatcctt cggtatattc cccattaaaa gcgacagcta gacctgtagc ttctcctcca
    20221 tgctcagaaa gttctcctga aaaaaagaat ggcaatagaa aaaagggaag aataaagaaa
    20281 agaagacaac ctagacaccc tttcttcatt tcttcctcat cctttcttcg ataatggttt
    20341 ttggactgtt cgtccttttg gtaaagtaaa atcagttcct ggttttgcgt ccaaaatcgg
    20401 cagtgtttta ggatcctttt ctattttttc cgattgctct gttggcttat ctaaagaaga
    20461 aaatgcaggt tgtaacgatt ttggtgcgga aatcgacggt ttactttcag acgaacgttg
    20521 ccccggttgt tgcacagaac gatttcctga tagagtggct accgacgaat taggaacaga
    20581 gtattggttc aataactgct gcattgctgg ataactccca tttttcgcct gatttttcac
    20641 ttggcgaatc tgttgtgaaa gagaaccagc agctgttaat tgtggcacgg gtacttttcc
    20701 agaaggcaac ggaatcgatt gaccaacaaa agcgatacct ttatttaaca atggttttct
    20761 ttgattattc ccttgttgct tactcaattg tgccaatgat tgtacctgtt gttttggtct
    20821 tactgaattc atggacgaat ttggaatcaa accatttgaa agaccagaat tccactctgt
    20881 tttcgatttg gtagaagaca tttcaggctc taccccatga taacgaacat gacgaccact
    20941 ataaggcaag gcttgatttg aacgcccacc tagttcaaag ggattaacca cttgcctccc
    21001 ttttaccgat tcttgtggtg tacgtttttg ttgaaacttt tgaaatccat gtatcgctgc
    21061 agaagcgagt ggattcgttg ctcgatacac attccctgct aatcctgcac ctttgattgc
    21121 caaattttgt ccagcagttg atagttgacc aggcagttgt ttcatttgag acaaagaatg
    21181 acttgctatg tttttcgtgt tgtttatagt ttgatctgca gctcctaacc ctagtttctt
    21241 taataaatct ttccgtttta caatgagtaa aattgatagg agtgctttga caaacatatt
    21301 tcctaaaaaa ccaaaaacag tattcattgg aattaaatcg tctactatgt tatagatcaa
    21361 aaatgccacc aaaataatca cactgtaaaa agatttttga aacatcaagc ccaaaaagga
    21421 ctttaaggta ttcatgagca tataatcaaa tactggaaca aaagaagcga gtaatgaaaa
    21481 aggaatcaca agtaataaac acaacaccaa catttgaaag ataaaacgaa tcatgccaat
    21541 cactacaagt ggtaatccaa tacttagatt aagcaatggc accatagaac caacgactgc
    21601 tttataaaca gctttagttg gttgaatata gacacgataa gccttctttt tccctctatc
    21661 ttctcctgca gcagtggtca cttttgtctc aatatcagat aattcttcac ttgacgcttc
    21721 cttggctaga aattcttgtg gttcaatctc tgcttgatac aattcttcta aatttgttgt
    21781 cccataattt aacaatatat acggatcaac aacagccgct ttataataaa gttggcgcac
    21841 tttttgaatg gcttcctttt gtgtcgttgg cactaaaaca ccattgacat cttttgtcgc
    21901 caattcatct agactatttg tagcagaaaa aataaagcct tctacctcca cggacaaatc
    21961 atttaaccct ttggtaaaag cttctccttt actaggtacg ttactaccat agccaaacca
    22021 aaccattgaa aaaatgacaa cgaagagaaa tcgaataaac accatttttg cgtaatgttt
    22081 ccctctaaat atacgaagag taaatagata taacacatac aagacgacaa tccccgcacc
    22141 aataggatcg tatatttttg aataaatagt atccgaaaaa gtgaaaaact ggtgaattaa
    22201 ctcgttcatt gcagctcctt catagagctt ctcaatgaca aaatcaaggc cttgccaaat
    22261 caatttactt aaactaaaga acatattcgt catcattttt agaaaatctg tgccatttgc
    22321 cccaaataac attttgccta atgaaaattc actatcagat aaataagaag aaaaggtatc
    22381 aatatgaaat ttcccaatct catagatcga ttgatccacc tgcttttctg ccgcatacac
    22441 caatccacca acaaaaagca gtccgaatcc aatcgttaaa aaaaacaaaa cgcaccattt
    22501 cttttcctta tgctttatat aagccattgt ttcacctcat ttctattgca tgattaacgt
    22561 atcctgttct tctacttgag agtgacttgt tttttccgtt gtttgtaatg cagcggtcat
    22621 ttcttcaaac aaactgtgta cattgagttt tcctacacgt ccatagggat ctaacatcca
    22681 acactgtcct ttaatcatgt ggctcatttc agctaagttc gcttcattta cttccaaacg
    22741 taaatgattc aaaatctttt ctcgctcact gtcttcatca aaagcaaaga cgacaccaaa
    22801 attccctttt tctttatctg aacctaagtc accaataaat tgtgtatcta acactaattg
    22861 attcatctgg gaacgcccca cacgtgccat tgctgcaata atactacgtc ctgcgttcga
    22921 attattaaat acccatgctt ctgtaaaata aatcgctgtt ttcttactac tatctcgttt
    22981 tccaaacatt tcacaaaaac gacctaaact aatcatcaaa cacaaagact ttcgatccgc
    23041 atctgtatat agttctggtc ggacattgtc atttggtaat ttcaaatctt ggatctctaa
    23101 aatcgttgtt ttggcattaa agtctaatcc cgggttttct ccataagaaa atcctaaacg
    23161 taaaatcgaa tcttctaccg tcaagcgtaa aaactcacca tattctcgaa cctctttttg
    23221 ttccatttgt tctaaatagt cgaccacatg catcaagcca acttgttgtc cggcttcttt
    23281 ttgaactaaa acttcttgga ttccctttaa taccgcccct tttaaaaggt gattggtacc
    23341 taacggactt aactcatcaa tcatatcctg ggctacttgt ttcgcatcta ctcgggataa
    23401 acaaacaatt ggatctaata caccataatt acggggattt gtagcatcta acgtgacata
    23461 actcattttt tgcaaatgtt ctacaaacaa agggtaatgt ttctgataat acgggtcttg
    23521 aatgagtttc gtaaaccatt tgcgcttttc tcctttggga tccacaaata aactttgtac
    23581 attcataaac gaactataca aaaagagcaa gccaactaaa aaggtcttcc ctttaccggt
    23641 ttttccagtt actgctacat gaggcgcatc aaacaacgca cctttgattc cttccgcaat
    23701 ggctagaaga ttcaaaaaga caaaacgatt tgacgcataa attaattctt ctaatggtgt
    23761 tttttctcct cgagaaccac catcccctaa tgtatcttgc cgaccaatat accagccgga
    23821 ttccatacct aattctgata ctgctgaaaa caaccattcg ccaactgctt ctacagttgt
    23881 tcggtgcttc caatgtaact gccgtcccaa tggttgccct aataaacatt tatagaacaa
    23941 ttctcgttga tcattttttg cacggtggat atggataatc gtatgaagtt gttgaataac
    24001 cgttgcacta ttttgacgaa cttcttcaac cgtttgaccg tatacaataa aacatgcgat
    24061 ccaatcgacg acaggttgcc cattatctac tcgttgcttt aaatgtttta ctaatcctcg
    24121 aacagttaat aagcgttcac taggttcttc atcattttga accaattcct tatgctctgt
    24181 tttgaaccgt tccgatagcc aattcagttt taaaccaata cctccctgtc ctttcaagcg
    24241 tggatagtga attttcattc gcaattctgc tgggaaagaa agatcctgag caaattgaaa
    24301 tagatcacaa tttctcatat tttcaggtgt ttccccaata ggcaacaatg ctaaataact
    24361 actccctaac tcatcttcta attgaagaag gcctttatgt tccttcggta tacgtacata
    24421 cgttccttga ttgtctttca gtacttccca attattttgt tcttctactt tgtgagccat
    24481 tcctcgaata tattgcagac gattgagata caacagctct ttttttacta agggacgaat
    24541 agaaaagcta taaagtaatt gtgtcacttc ttttacataa ggatcacttt cagaaaacaa
    24601 agtttccaaa tcatctactt ctaatccaaa taagcgtgct aaacttcctg ctaaatctaa
    24661 ccagattaga tttattacat catgaaaact ctccaccgtt gctttttctt tgactttgat
    24721 tcctaaaata aatgaaggac gatagacttg accttctcgt tccaacaatg tcactgtttc
    24781 ttttgcataa tacgcaccaa ttgcttgact atcgggagaa aatccctttc ctaatgtgtc
    24841 aaacttcgtc cgtaaattca ttgttcgtgg atacatcacc aactccacat cttcataatc
    24901 tgctaaaagc aacagtaaac gagccatcat ttcttttatt tcctgtgtct tttttggtgt
    24961 gttttttgaa attgttcgtg gaaaaacatg atagtaggcc cagatacaat cctctccatt
    25021 taacaaacaa tttttcttga atgcttttac tggttctttt acaagcacca atgaatttca
    25081 cctcccaata aaaaaggaac aaccagttga ttgtcccttt ttctaatcct ctaaataaac
    25141 atacttattc acaaaaagta attcttccaa tgcattgttt gataccttgt cccagttata
    25201 atacacacaa tcaccattca tcatacgaat catataacaa aaccaatccc ataaataaaa
    25261 atgcactttt tttccatcag gttttagttt tgccagtgct aacacacata aataggggag
    25321 tcctatataa aaaacaactc ctgctggtgg aaaaaaagaa atgacccaac caataaatga
    25381 acgtaaaaat aaatattcaa agattacgac caacaaaaaa gttgctgcat tgactaatgc
    25441 aactggaaat ggaagacgcc aattgcttgt aatctgttgt acgcgataag gttccgcata
    25501 aacaatcgaa taatcataag gttcccaatc cacttcacta tcctcctaat ccataaataa
    25561 tttttcaatg attttaccaa acgcatcaaa tacacgtgtt ggatcattta agaagaaata
    25621 aactgcggca cctgctgcga tagatattaa tgcatttcct acttttgatt tcataaaatt
    25681 tcgcaatccc agataaacaa ccaccattat taaaaaatag ccaccttgcg ttaatataaa
    25741 atctacaatt cccttcagtg tcatttccgt tcttctcctt tcctatccaa ccaattatgt
    25801 gttaattttt caacataata ctgatttgtt tttttcgtaa tgacaatcgt catttgttct
    25861 ttttgtaaaa gtgacgtatc accatctcga aagacgaccg ttgcttttac aataaaaggt
    25921 tttttcgttt ctttataacg caatactttc atctctactt gatcaacttt agctgtttga
    25981 ttgagtcctt caggttcttt catcatgtat gacatatctt ttgcattgtt tttcgcatac
    26041 ttatctaaga aatcttccac aaatccttca acatctttcg cttccgacgc atcaaccgtt
    26101 tcgtataatg tgtcatcata agtaatcatt cgttgttttt ctgtattctt tggtagtgcc
    26161 gtaaaataag gataagcaat aatacgacag cccttgtttg aaatagaaaa gggaagattg
    26221 agtacttgtg tgattgtcac acttgtttct ttcttactct ctggattcat cgagcgaata
    26281 tcataagtta ctttataact agcagttgga atggtttcaa tttcttttaa tccaataaac
    26341 gaagcagcag ttaattttcg aacacctgaa acacttcctg gttcttgaat aaagttttgt
    26401 gcataaaact ctttttttaa tgtttcttga cgttgttgtt cttgttcatg attatcgaat
    26461 gtattcatat acaaagcaac aaaacgattc ataaaggatt caatttcagg tgtgtacgtt
    26521 aattctgacc gactttgtac ttctttcttt tgtaattgtt ggatttcatc tttcaaaaac
    26581 gacacttgaa ccttcgtttt catggattga aaaaatgcca agccccctga aaaacaaaga
    26641 aataaaacca aacaccaaat ccatttcact gctttttttg aatggatttt cttttgcttc
    26701 ggtactttct ctgtataaac tttttctttc cgccgtagtt gtcgtttttt cataagctac
    26761 tccttctagt atgtttttac tagtacgtac gattgacaac gataagcttg tttttctgaa
    26821 aaatagtcca ttcaattacc ctcattttct attccttttc tctttctttt atatccaaca
    26881 aatccagcaa tacttgtcaa cactcctcct agaacagaaa gtgcaacatg tatttcttca
    26941 ccagtctttg gtaagacttt tgtttttcca tctgctgttt tgatttcaat attgccactt
    27001 ggaagtttct ttacaggaat cgacaaatcc tttaccaaat tcccttcact atcttggatt
    27061 aatggaatac catcttttac accaacaatt gtttgatcac ctgctacttt taatggggtg
    27121 gttggtgttg gaatatgggt caattcctct acattactag tgacaatcgg tacactcatt
    27181 ccttgagaag tattcgtcgt aatttcaccg cttggggtta ccgatactgt gacttctttt
    27241 gctgaaacaa taggtttatt ttcttttgga attgcttctg atttttcaac aggttgtttc
    27301 ggttcttctt tttgttcttc actggtagac gacatcgttg gctgttccgt acttgacgaa
    27361 tcagtattct tttcagaaga actggttgtt cctgattgtt ccggagttgt gactatactt
    27421 gaatccgttg ttgttgaact atgcacacct tcttcttcga tcgaatcagt cggtaaagtt
    27481 gattcaggaa actctggttc tggtgtcact ggctcttttt caggtttctc tgactgcaat
    27541 tgttcaggtt ctgctggttt ttccgttgtt cctccattta gctctgtctc aacatctggt
    27601 aatccttccc ctggcaatgt ttctacaatt tctgacttag gtgtttctac agtttcatct
    27661 gcataaacca atgaactccc taattgaaac gaaaataatg aaattcctag tgttgttacg
    27721 actgctaatg ttgttttttt catgattatt cctccaattt tttatgcaat tcgtgttttg
    27781 ttcatagacg ccgcactaat attatctgaa cgataaaaga actcccgtcc gttttcttct
    27841 aacccaacat atggattatt tttgaataat tgaatcaata gttcactggt gttgtatttt
    27901 ggatagtttg caaaaaaata atactgtgct tgtcgcccat ccgaaaattc aacaagtaat
    27961 tcataacgat accgcttcaa tccttttcgt tgttttaaag gatctctata ccaacttgtc
    28021 catcgttcca ttcgtggata gagatagaca cgatacacat agaacaatgc cataccacat
    28081 accaccaaaa ataataatcg ccacataaaa attccccctt ttcttctacc tttcttgaat
    28141 catcgaacat tcataccctc tatagagatt tgaaaaatcc atcatcaatt gttcatataa
    28201 acgttgttgc aaatagcgat tgtttcgaat acataattct tcatagccat caatcacttg
    28261 atcttctttt tcatagcatc ttgtagctct atgacgctca gcttgcaaga cataaccaaa
    28321 ctctgcacca tatcgttcta ctgttaataa ctgttgacta cgcaataatg caatataaca
    28381 atcacaaaaa tgttcaaccg acatttgatt tttctcacgt ttgtgaaata ggtattcttt
    28441 agaaagcaca ttattcgtta aacaatctga taaatagcga taatctggcg gtcggttatt
    28501 ttcatctgtg aatttttccc ataataaact gatttttatt cgttcagcgg ctagatagtc
    28561 ttgttcgcgt ggataaaatt catcaatcgc acaaagtttt ttatcacctg aataaacaaa
    28621 tccatataat gcgaaacgaa ctcttccttt tcaattggct ctcctaaaaa ttccagtgac
    28681 ataccatctt ctccttccta aaaaaaataa aaaagcagaa accgatttgg cttctacttt
    28741 tctttccttt tatagctctt ttttacggaa gaatccgcaa atgacatttt ttcaaaaaat
    28801 caatcggcac aagttcacga aaacttggag caattttgga tagaatcact tccccatctt
    28861 gtaaaataca atcataaaaa tcatcagtta cttgatactt acgcttctta caataattga
    28921 taaaatagta gcgatcattt tctatttcca ttcgttgttc tactaagcgc atacgaacat
    28981 catcatagaa ataagccaca aatcgccctt caagttcatg tttttcaatg ggatactctt
    29041 ctggtaacaa tctatttttt cgtcgtttat acattgctgg ataccttaga agaagttcat
    29101 ctaaacgtct gtcagtatat ggtaactgac gaatcaacga ttctaatcgt tctttataca
    29161 gatagaataa ttggcgcaag caatagtgta ccgcctctgg attttctaat actgttggat
    29221 cacgtgacca atacgcacca acataaatca taattttttt catttagaca atcacaccag
    29281 caatactaat gaccgatcca caataggtag aacaagtggc atagcgacca atttcctcta
    29341 attctatctc aatctcttct aaagaaagat atgtctgttc ttctgttggc tggcgactca
    29401 tttcccaagt gacagtcacc aatgaaattt cttccataag aggtttttcg ctgctaagtg
    29461 ctgccacaca gttttcatat atcttcattt tatcgcagcc ttttttaacc gctaaaatcg
    29521 caggtattgg caatgaaaat cgataaaagc gaatgaattc tagccttcct ttccttcggc
    29581 taaattccaa ctcttcgagt tcttgttcgt atcccttacg aacttctaat aagcgtgcaa
    29641 tttcttgcca gttctctttt tcctgataac gtccagcttt taccgcttgt tcaatataag
    29701 gaactcgaaa acgaaaataa gacaaatcaa ccatcttttc atttaatgct tcgattgcag
    29761 cctttaaatc caacaggcgc agccattctg ttgtcgtata tgcattcatg caattttccc
    29821 tcctgacgtt cccatccatt ctagatagtt ttgttgctcg tcatttagtt cggcattatc
    29881 aatcccttct tgtacaaagt ctcttcctaa aatacgatct gcttcttgta acaactttaa
    29941 tgtcggagct acttgtttca aaaaccagcg ttctgaacga tctaatgaga atttttctgg
    30001 ctttactgta aaatccaatt cttccatcga acgaaataat tttgcccatc gtttatcaac
    30061 aatccgtgca tcactttcca gtctatccca aacaataagt tttgcattaa ttaaaccaac
    30121 gccaatctct cctaaacgtt tcggtgaatc acctaaaata aatgcttcta ctgcttgtgt
    30181 tgctttctca ttggctaacc gtaactcata tcgattataa ataccatatt tcgttaatgc
    30241 ttctaattct gaaatttttt ctcggttcgc aatttcaaaa cgtttctcat aaaaattaaa
    30301 aaagagagga ctctttcgcg atccaaaata caaagataat ccttcacttc gaaaatagcc
    30361 atcatctaaa aaacaaccgc caatattctg gaatctctta aagtccattt ctaataaacc
    30421 tgctttttgt ttttctagta attcaaagag atcgaaatgt tcttccgttt ccaatcccca
    30481 aagttcatct aacgcaatat cgaatcggct acattgcaca cgtgtataac cagcttccaa
    30541 ctttccttga tttttccaaa tggaatatag ccatgttcgc caatctgttt gttgttcacg
    30601 catgacttcc tcatattccc ggcaaccttg tcctgaaaat tgcacaagta cattttctgt
    30661 tacagttcgt tttgaatgcc agaacaccca gatattccca cgaacagaaa gacagtcaaa
    30721 aagatacaag cccgttggac gtgtttcaaa ttctgtcaga tccattcgta atacacgttg
    30781 aatcactgtt ttgtagtctg tcccaaaaaa ggaaagacgc aaataatcaa tcattgctcg
    30841 aagatctatt ccgcttttca tatacaatcg aaagcgttcc ttgattcgtt gttcaaaatc
    30901 ctctgggatt tttcttttcc cttgttccat ctttctaatg taatcttttg agcagcccat
    30961 caattgcgca aatcttgctt gtgatagcgg ctttttatct ccacttatat tatgaattgc
    31021 acttaacttc tttctgtagc tttttagctc aatcggtgtc attttctcgc tccttttata
    31081 aaataacaaa atcgattttt ttaacccttt agaaacaccg atatatcaac tagtccccct
    31141 cgttttttgg gaaaagtagt acaactgctg accttagata atcacttgat acaaaagggt
    31201 ttataaaact tttttctatg tgttatgtta tttctgcccc ccatattata tatcgggggg
    31261 ctttacacaa agaggccgtg cgcaagcgca cgacctcttt gtgagctgca cacgctgcta
    31321 gcgagtgcgc attataggcc catgcttcgc agccttaatg cgcaccgcca ggcagagtgc
    31381 ggctaccaga ctacgtctag agccctcccg atagataata actacaaaat taaatgatct
    31441 aattttgtta tttcactata tttttcagga gaaaaaacgc gataaattga caaagttaaa
    31501 tcctcttcta aatctactcg ataatgatac aatcgagtca atcgttctat atcttttttc
    31561 gttagcaata cttgattaat aacttttcct gtaaaaacac gttctttgcg caaaaagcgg
    31621 taatcagtta cttgttcaat acgattcaac caatcataaa acgttctgga agaaagagaa
    31681 ggattaatca ttcgtattgc tgtagctact ctataataac aactctctgc catttaactt
    31741 cacctcctta gttgattaca aaatatgcgc atcagaaaaa cttgttcctt ttggatcttt
    31801 tgctaaatac atttggcgct ctagttgttc ttgtaacaac tgattatcat ctcccatcgt
    31861 ttgatcttga ctatcctctt ttactgtaaa atcatagaca atcatttcag taaatttctc
    31921 aaattctttg ataaagtgat aatctttgta aacaaatggc gcaaaaaact ctacaggtac
    31981 atcaaatcct ggaaaagcat atccacgacc aggttgatct ttgttgtaaa atggcttatt
    32041 tttaaaatct tctccaaaag tcattaaata accaatggga gacaatcgac caacagaaat
    32101 acggaatgtt aaattatccc gaacaccact tggaaaatat tctgcatctg gtcgttgcat
    32161 tgctaaaata atgtacatcc ctgcttgacg accctttaat actaatggct gaatcaattt
    32221 atcgactcta tttctcgctt ccatatccaa cgaaccataa aaagctgccc attcatcaaa
    32281 aaagataaaa tgcggtggta acccataata agcataattt ttccctggtt catatttagg
    32341 atctttcatg gttttcatat aggcataccg ttgattcatt tcttttaatg catttcgtaa
    32401 acaaataatc atttcttcat ttattttgta aaacacatgt cctttgaata catcgacgtc
    32461 acctaatgct gctaaatctg ccgcttttcc atcgcaaata tctactgtcc caacacttaa
    32521 acaagcttga attaaagaat acaataaata ggttttccca ccaccgattg caccagcaat
    32581 caacatattt ggtgattgat catataccca atccaaagtt ttcattaaat gaatacgtcc
    32641 attttctgct atacattgat caataggaat ccgaccagaa tctgaaagga atacataatt
    32701 gattcgggct ttttctcgtt cttcacgaat taaatcacgc aaaaatactt cagataacaa
    32761 atgacccatt tttaaaaatc gttcgtgaaa acgcccacca tctaaaggaa aagaaaccac
    32821 taattcttgt ttcttttgat aaaaataaac ttttggtaat ctcatttttt cccttgtttg
    32881 tttctcacta acttgtttct ttgtataaac aaaccgattg tcaattaaca ttcgaacaag
    32941 taactgacgc caataaacag aggcaaaata tccttctttt agtcgcgacc aatgcatcaa
    33001 ccctaaaatt aacccactaa ccagcaacaa atacaacaca atacttattc caggaataag
    33061 atacatttgc caattcgaaa acgtacttaa acgtggataa aatactttcc aactaaacca
    33121 acctaacaga atcaaaaatg gaaacaaaaa gaaaccataa tagcaaaacc ataaataacg
    33181 attgaaatag cgaattcgcc gaccacgata cttgaaaaaa tgagctttca attgtcacat
    33241 tcctcctttc tgtttttact tttcctgtgc taaaaacttt tgacaaaaag caactaatac
    33301 aggaatatca catttagtaa aaaaacataa tcgaaaaaga tttaatcgat ttttataagg
    33361 gtgtgcacac atattacata ttcgtactat acgaatccaa atcacttcct gatgatcttg
    33421 tttccactgg tacggtaatg aaaaacgaac aagatcacgt aaaaacatac actgcccaat
    33481 aaccgcacaa atatatgcat ttgtttgtac aaaaatcgtt ggattgtctc caaaatactc
    33541 aatcaattgt tggatatttt gataacaagc acaatctctc aaagtatttg attggtttta
    33601 tctgaattca aaatctcccc ttccttctat cttagataaa aagaatagga agactccttc
    33661 ctattctttc aaaaaaacct tattttttct ctttttcctt agaatcaaca cttggttgtt
    33721 tttgatctaa acctttttct tgtgattgtt tcatattagg attatttgtt ggagcttcta
    33781 ctttctctaa tcctttcgca taaatcacta aatctgaata ttcacgattg ccatcttgtg
    33841 ctggaatata taccacatga tattctggat caattaattt cacaggatca cgtcgtttaa
    33901 aaccagtcaa atctacatct gctggaaatt taactcgtcg tgcaatcttt ggttcatctg
    33961 gatttccaaa aactaattgt gctcgctgtg atcgaatttc tcccgtataa tcgcctgttt
    34021 cattatcaaa tacacgatca gactcttcta atgcgccaaa gcgtacggct cctaaacaag
    34081 tttcatctaa tgcttctgta atgtcataat tatttggaaa ttttaatccc atcttacaaa
    34141 acctccccaa tttttacacc agttaaaaag acaccaacat cttgatagcc agttgttcga
    34201 cgctctgttt gtcccatatc atttgtttca cgactattca ctcgttcttt acgaaaaaca
    34261 aaataagctt cttcaaatga aatacgcacc tctttttctg gtaatcgtaa tcttttatcc
    34321 tcttcagctg tgagataaac aaaaaaagca ggctgttctt ctcgcgaaca ctctgcttgt
    34381 aattgaacat atcgcccaga cacaccatta atttcctttg gtggattagg ttcccgtgaa
    34441 cccaataaaa atacttgacc taaaaactct ttcttcatac gcaatgcttt ttctgccata
    34501 aagtttttcc tcctcttaaa aaccaaaata aattctaaat atttctacat tacaatcaaa
    34561 caagcggtta aaaatcctaa ccgcttgttc ctcatgacta atctttggct ttacgtactt
    34621 tataaagtgt tatacctaaa gcaatgacta atactactaa tccaagaacc ataagtatac
    34681 tacttgtttg ttcacctgtt tttggcaaca ttggtacaac agtagctggt ttaacccgtt
    34741 gttctggttt cactggttgc ggtggttttg gcgcttccac aatttgaacc gtttgatctt
    34801 tgtttgttag atctacattg tgtttcgcca cttcttcaaa cgtttctttc tcatcttccg
    34861 ttttcgcttg gagccattcc gcaaaactca agccttttcc tgcaagattg cgaccgtcca
    34921 ctaagaattc aaataagaat tccatcattt ctttatcggc cgcaaagact ttttccgcat
    34981 gagtgccttc aaatggtgtg ccgtctggtg ttaagtagcc ttgaacaatt aagcgataga
    35041 tttgaccaat cgcaagaccg tctactttga cccattcaag cacagtttga tttggcaagg
    35101 ccaagatctc ttttccgcca tttttattcg tcgctttcgt cgtaatggtt ggattactga
    35161 ttttgatggt ttggtcattg tcggtaatat ctgcgtgaga agccaataat acgccttctc
    35221 taaacagatc ttcaaagacc acgacttctt tgcctcgcaa cgcacgtgcg tctagaataa
    35281 agtctagatt gactgtacca tccgttgttt ctgccacaaa cgttaaggta cttgtgactt
    35341 cttttccatc tactagcact ggtttttccg ttactttgtc cattagaaca ccgttgactg
    35401 tgtatgtttt tccaacgacc aaatcgtggt atttcacagc gtctgtaatg gtgactttcg
    35461 ttaatggatt aacctctttc aagccagtgg gtttatgtgt agccgttgta cgtacacttg
    35521 ggttagttac tcgtaccgtt tgccctttgt cgttgatgtc tttatggttt gctagttctt
    35581 catcatttct aaaaagactt tcaaacacga cgatttcacg accatttata cctgtcaaat
    35641 caaaggtaaa tggtacgatc accgtttcat tttctgcttt ggcaataaag ccaacactcg
    35701 tttctaccgt atttccatgc acaagcaagg gttcaccggt tgctttatcc attaagatcc
    35761 ctttgaccaa gtatgctttt cctggaagaa tctcggtaat ttcaacggta tcgtttaagg
    35821 taaacaatcc tgttgggtct gcgactgtca atccgtcttc aaagacagcc gtggtatgaa
    35881 cggttggatt attcactttc acactttggg cttcatcgtt gatatccgca tgtgctgcaa
    35941 cttcaatgcc atcacggaaa agtttttcaa aaacaacgat cgtttgacct ttcagaacac
    36001 tagcattaac cacaaattca aggtcttttg tgccattgac tttgtctgca ataaaggcca
    36061 aggtacttgt gatttctttg ccatcttgta aaattggcgc attcgttgcc ttgttcatca
    36121 atactcctga aatggtgtat tcttttccga caaacaagtt ggtataagaa acactgtctt
    36181 taatgacgat cgtttctaat ggatcaacca ctttttcacc atcggcttta tttgacgctt
    36241 gtgtttctag ttttggtgga atcaagtaat tacgtacaga aaccactaac gtttccccat
    36301 catctggcgc attttgtttg aatgcaaccg tgaatgcttg tttatctgta tttggttgga
    36361 aactttctaa tggggcattt tccaagaagt aatagtcatc ggcaactaga cctttcacta
    36421 aaatttcccc gttttgatca gtggtatatt caccaattgc ttgattttta ccgttataca
    36481 aggtaaagtt cacacctgcg agcggcgttt tcttcttgtt ccattcttca tacttgaata
    36541 attttacact tttccgtgct tgacggttcg caaaatggat cactttgatc ggttctgtat
    36601 cattggtaat cgagaacact aatgggtttt gattcaaaac gtaatttacg ggggctttga
    36661 tttcttccaa acgatagcga tcttttccat aagtaagtgt accgttagtt aggaaaaagc
    36721 ctttttcatt ggttttaaag atatccgttg tttctgtatc atttggaatc tccattgtga
    36781 cataacgatt ggctaaagta tcaaaaatct tgaattctgt attagcaagt ggaatcactt
    36841 ttcctgtttc ttgatccact ttcaccactt taagacgttg ttcaattact ttatcttcta
    36901 aaacataatg gaaagtttgc ccattttcag aaattgtgac ttcaagtggt tcaaatagtt
    36961 tatacccttc ttttcctttg gtttcagaaa gaatgtacgt atcaaagaca acggtatttt
    37021 taccaaaagt agcataacca tcattgtctg tcacaacggt tttcaccact ttgccagttg
    37081 tttttgacgt caaagtgatt tcaatgccag ctaattttgg tttgttatct ggattcgttg
    37141 aatcgactaa atctttatta gcaactttca ctaaatcaat ggttcctttg atcacttgat
    37201 cttttgcggt cgcattgaca gtttttccag gttcaacaac aatttcaatt gcttcatcag
    37261 aaagcacata accttttggc gctttgattt cttttaaaac atatttgcca ggatcaagat
    37321 ctttaatttc tgcctgtact ttgccgttaa tttttttcaa ggtaatttct ttaatctttt
    37381 tcccatctgc ttgatacaaa ccatagaccg cattttctaa agaagctgca ccttgtgctt
    37441 tcacaccagt ttccgcatct tctttaaaca cacgaatgtt tccttgtttt agaatttgta
    37501 aattaataac agacgcatta ggttctgttg gatgtaacac accaattgtt tgagctcctg
    37561 gacgacgata ggctaatggc gcaccttgaa ctttaatttt attcaattga atttttgctt
    37621 tatccggtgc ctcttttgtg actgtaatcc atacttcatt gccgtgaata gatacattga
    37681 caccttttgg tttttgaatg tctttcacat atttcaaaaa atcattttta ttctttactt
    37741 tcaacgtttc gccaattttt accgtatgtg tttgattatt aaaagttgct ttttcaccaa
    37801 agttattaat caaacgttga atttctgctt ttcgacgatc ataatcaaca cggatatttt
    37861 tgattgggat ttttgtgccc aaagcttccc aaatcataaa ttgtgccgca atgtattgtt
    37921 catcggattt gtctttattt gtgacatatc ctaaataaga gatcaagctt gtttttttcc
    37981 gtacatttga atttttcaaa tagtttttca aagcagtagc tgtataaact tgaccttctg
    38041 cggctgagac accaggctca atacaaaacg ccacttgtcc attaatctca aaacgaacca
    38101 tataatttaa agtgaagttt agattgttcg gactagaagt aacaccaata ttttcgcccc
    38161 aatcaatttt ccgaccacct aaaacagctg cattggttgc acggcgaaca cgaaccgaac
    38221 gattggttgg tactgttact aacagtgtag gtgtacctgt ttttggaatg ttctgctctt
    38281 ttttcttttg attttcaata ctagccgtag attctttgtt tggtttcgtt ggttgatttt
    38341 gactagctgc ggttgaagag attgtttttt caacgttttc ctcatttttt ccttctttgt
    38401 cagcagtagc cgctggttgt tccactgttt cttctattcg tttcttttct tccgcttgtt
    38461 tctcagaaga ctctggctgc acagaaggcg ttgcttcggc agctttttct ccttctagag
    38521 tggacgtttc tgaagcaggg gttgtaattg aggcagtttc ttcaactggt tgtgaagtac
    38581 caggactatt ccctgggtgt tgtgtttccc caatcgtctg atcgagagtc gttttggaat
    38641 tgaccgttgg cgcttctgtc acaggggcct gttcaacagt ggcttcacca gctgggaccc
    38701 cggcttctgc tggttggact tctgtcactt cagaagcgac ttgggcaatg gcttccatcg
    38761 taggttgtat agccgtcgtt tcttctgcat gtgcttgagc agttcccacc aagaaacaga
    38821 attgagaaaa agccaccaat gcgagtgctt tcaaccaatt cattttcgtt tttgcataca
    38881 caagaattcc tccttttcct gagaatttta tataataaaa caaaacgccg ttaccaaaag
    38941 tgataacgac gtgaactgtc tcttctattt ttatctggcg ctcttatctg aacacatttc
    39001 atcaatgatt ttaccctccc actataatta cagaacaaaa aaagaacctg cttcgtggaa
    39061 atcaactgtc catttcccaa ttggaatacc agcttttttg gcaacttcac catcaccaaa
    39121 tctcatgcca taccaattac taccttcatc cttaatttct ttccaaccat atgacatagc
    39181 ttcatctaat gaatcaaaaa tcatgacact attgccaatt gttacaggat ctggcttaag
    39241 atcttctgga agagaaatat caatcatacc ataatgatcc gattcctcag tattattgtc
    39301 attactagct actggtttgg ttgttctact ggcttatttt gatacaacaa gtactcttat
    39361 tcttctttta cttctccgtt ttcaatttcc cattcaaacc actccgcaaa gtcttgctta
    39421 aaaccatcta ctcgatacct attttgtaaa taggcatctt ctgacgtcgt aggatctaaa
    39481 taggtaggtt tattctctgg acgagcatag acacctaccc atgtttcgta atctccttca
    39541 aaataactat ctaccaaata gccttttttt ctaagaatct cccgaacttg atttttaatt
    39601 attgatactt gttcaaaacc atcactcatt ctatcacctc aaataactct agattctcat
    39661 aaatattccc gacaatttca atcctatcaa tgatctctaa caaatcgcta cttatgcttc
    39721 cccattcgta taagaatttc ccttccgcaa aataaatttt ccataaactt cttcatgatc
    39781 gtcccaacca atatcactct cgaaaaattc cacgttgttc ttatctttta gctcagtaaa
    39841 tgccataagt tcaacatgtt ttccaacctc gaaacaatca caccacccat caaccgttac
    39901 acgtgtgcct tcaataccta agaaactttg tcctcgtgta ttaaacacta aaactttccg
    39961 cattttttgc tcattaatta atcacgcgcg aaatctagga atcacaactt attcgcttct
    40021 tctttataaa taaaaaaact ccaataatcg gaaattacta tcttttcctc attccttttt
    40081 taaatcttca tacttcttct tatctttgtc ctaacctaat aacacaaatt ttgaagaaaa
    40141 actcagtaaa acctgattct ttttttatct tcacaacaac caactttttc ttcagatcct
    40201 tttactagaa cttgtataca aaaattgttg aatgctgata taatttgcgg ctacttgaaa
    40261 aaacgataga tgtaaaaaga taaatagaat cattttttat tctatatcag tctatgattc
    40321 attgcttatc aatcacccaa cccattcgat gccgtttttt ttagagaaat ttaaaaaaga
    40381 ccgtacgaat ctcgcattat ccgattactt ttccagaaaa gatccccaat tctgtataaa
    40441 taaccctatc ttcttgttgg tttcattttc tcatataaga attcagtcaa tctacccact
    40501 atgaccatta ctacgtccaa cacaactgac atattaacca cgtaagtatt ccctcacaga
    40561 tctccgaatc tccatggtat ggattcccat cctgacgctg cagaggacaa catatttcat
    40621 gtggctctgt acccttttga tttggaaaca ctacaaatca atactcatct ataacttatc
    40681 aagcgaactt tcgctaggct tctagtgatt actaaattgt tggataacat acatagccca
    40741 ttttatctag ctgttattca taacagttag ttcttgtatt acttcttccg cttgtgttgc
    40801 cattgagctt gtcaaaaagt tacttagaaa aaagctgcca gtaaagcaac tttcaacaaa
    40861 ccctttttgt ttcttatcat atcaagaaac ctccttttat aaattttttc tataataaaa
    40921 aacgtcgata acttaataag tatcgacgta aactattttt atatcccgta aatcaaaaat
    40981 tctaaagaaa gatttcctat ataagcatat tggataattc gattagtatt gggtatatct
    41041 ctaccattct catacctatc tatggaccat tttgaaattg aaggagaaaa aagctttcca
    41101 aactcctccc tttctagata ataatctttc ctaattttcc tcatccttaa tcccatacta
    41161 aaagaattta tctttttaaa atcgctaact ctttcatctt ttggaagtat attcttataa
    41221 tcttctccgt acataaggta aggaacagtg accttaccaa gataggcaat attcattagt
    41281 ttttcaatat ctggaatatt aatacctttt tcccatctac ttataacatt tttgcctgta
    41341 tagatggtaa agtttttacc aaatttttca agcgtctctt cccggttctt tcggacttct
    41401 cgtattcttt ttcccataac aattttatcg tcttttgata aactaaatat ctcactatca
    41461 cgattaatct ccttcaaaat ttctttttca ttacttaata gaaaaatatc gatttttttt
    41521 tgcatttctt ttgacggatt tctctttcta tttaacacat aactgagtaa tacttcacta
    41581 attcctatat tatcagctaa ctctttgtta agtaacttat tcatatctct gtatgcaatt
    41641 agtttaccga tgagaatctc aatcttatct tcctcatctt ttattagagt ttctatattc
    41701 agaatcttgc ttagagtatt atctacgtct ttagggattt ttcttgaccc tttttcatat
    41761 ttaactaaag tactttgtga aatacctgct aaatcagcta actttttttg agatagtttt
    41821 ttctcttttc ttagagattt aagaaccctt ccacttgaat tatcaaccat gaaaaaagca
    41881 cctcctatga gatgctttca tcatatactg acagctaaaa atcttcaaga aaaatattcc
    41941 aattttgtta attttagtat cgccgtgata accaaatgac agtttaaaat cttttataca
    42001 gataatcctc agtgttgcat ctactcaaaa aggtttattt aataggtttt tcgattgatt
    42061 ataagttatt tcacactatc ttgctcatcc attttcagaa cggccatgaa ggcttcttgc
    42121 ggaacttcga cggagccgat ttgtttcatc cgcttcttcc cttctttttg tttctctaag
    42181 agtttacgtt tacgagaaac atctccacca taacatttag ccaagacgtt tttacgcaag
    42241 gctttgatgt ctgaacgggc aacgattttt tgtccaatag ctgcttggat tggcacctca
    42301 aattgttggc gtgggattag ttttttcagt ttttcaacga ttgcttttcc acgttcatag
    42361 gcaaagtctc ggtgaacgat aaagcttaat gcatcgactt tttctccatt cagcaagatg
    42421 tccatcttca ctaacttgct tttttgataa ccagacattt cataatccaa tgaggcatag
    42481 cctttcgtgc ttgatttcaa tttgtcgaaa aagtcaaaga caatttcaga aagcggaata
    42541 ttatagacga catttactcg gtaatcatct aaataatcca tcgtaatgaa ttctccccgt
    42601 tttcgttgag aaagttccat tactgcaccg acaaaatcat ttgggaccat gatctgtgct
    42661 ttgacgaatg gttcttctac atcctgaatc gttacaggtt caggaaaatc tgccgggtta
    42721 tctactgtag cagtcgtacc atccgtttta ttaacatggt aaataacgga tggtgcagtc
    42781 gtgatcaatt ctagattgaa ttcccgttcc aagcgttctt ggacgacatc catatgaaga
    42841 agtccgagga atccacagcg gaacccaaaa cctaatgctt gagaggtttc tggttcgaat
    42901 tgtaaagcag catcattcaa ttgcagtttt tctaaagctt cccgcaaatc gttgtaacga
    42961 gaagtatcaa ttggataaag accacaataa accatcggat tcatttttcg gtaacctggt
    43021 aaagcttcag cggcaggatt gtctgctaac gttactgtat cccctactcg tgtatcttgg
    43081 acagttttga tacttgctgt gatataaccg acatcaccga ccatcaaaaa gtctctagca
    43141 actgcttttg gcgagaagac cccgacctct gtaacatcaa atgttttgcc attactcatc
    43201 agtttgattt tgtctccggg tttgaccata ccgtccgtga tccggacatt tagaacaaca
    43261 ccgcggtaac tatcataaat agagtcaaag atcaaagctt ttagcggtgc atctaagtct
    43321 ccacttggtg caggaacata ttccacgatc tgctcgagaa tatcttcaat cccaatccct
    43381 gatttggcac tggctaatac agcttcacta gcatcgattc cgattacgtc ttcgatttct
    43441 tgacggacac gttcgggatc tgctgcgggt aggtcgattt tattgatgac tggcaggatc
    43501 tccaaatcat tgtctaacgc taaataaaca ttggccaatg tttgtgcttc gattccttgt
    43561 gctgcatcga ccactaaaac ggcaccctca caggcagcaa ggcttcgaga gacttcatag
    43621 gtgaagtcga cgtgccctgg ggtgtcaatc aagtgaaaga tatatgtttc tccatttttc
    43681 gctgtgtaat tgagttctac agcatttaat ttaatcgtga ttccacgttc acgttccaaa
    43741 tccatcgagt ctaacagctg atcttgcatt tcgcgagacg tcacagtgtg cgtcatttct
    43801 aaaatacgat cggccaatgt agatttccca tggtcgatat gagcgatgat cgaaaaatta
    43861 cgaatctgct cttggcgttc tttcattttc tttatatcca tagcgtctcc tcttttcata
    43921 taatcagcac tttccattat accaacgaaa agcagtaatt taaagagttt ttaattagtc
    43981 agctatagaa gttttttttg tagaattttg ctatactgtt atcaagctaa taaatggaga
    44041 tgaaaaaatg gatcaaaaag attttagaga aaatcttgaa ttaaaaccgg tcactcaaga
    44101 acatatcgat caattcaatg aattgctttc ctatgtgttc caagtgactg aagcagacat
    44161 cgaggaaagc gggtttgaga ataaacgagc ttttatccgt tccaaaaaac cgattttgga
    44221 agtatcgaaa gtgttcggat ggttccacga aaataaactg atctctcaaa tcgctatcta
    44281 tccttgtgaa gtcaatattc acggtgcacg ttataaaatg ggtggtgtga ctggagttgg
    44341 aacttatcca gaatatgcta accatggatt gatgcaagat ctaattatcg ttgcattgga
    44401 taatatgcgc aagaataagc aatggatctc ttatttgtat ccttacagta tcccctacta
    44461 tcggcgtaaa ggatgggaaa tcatgtctga taaactttcc tttaaaatca gagatacaca
    44521 gctacctaaa caaatcccag taagtggtat ggttgagcga ttaccagttg atcatccaga
    44581 cgtattttcc gtttatgaca aatttgccca acaaaaccat ggtgctttat tccgcagtga
    44641 ttttcattgg gaagagtatt ggcgtttcga aaatgaagat gagcgcacag ccgctgtcta
    44701 ctacgatagc aaccatgaac caagaggtgt tctgttctac tgggtagctg aagaaatatt
    44761 tcacgtaaaa gaaatgttct atcttgatca agaagctcga aatggcttat ggaactttat
    44821 ttccgcacat ttttccatga tctactgggt acatggagat atttataaaa atgagccttt
    44881 agcatttttg attgaagata gtcagatcaa agagcagatc gaaccatatt ttatggctcg
    44941 gatcgttgat gtaaaagaat tccttcaacg gtttccgttt gttggaacag ctgatgcttt
    45001 tcatttcatc attgaagatc ctgtcgctcc ttggaataat ggtgtcttcg ctttaacatg
    45061 ggatgaacag ggacaagtcc gtgtcttaaa tgaacctata ggaaaatccg ttcgactgga
    45121 tatccaaaca ttgacttgct tgatgatgaa ttatcgccgt gcttcttatt tggcaagaat
    45181 cgaacggtta gaaacagatg aagaaacact gaagtcacta gagcgtatca ttccaaacat
    45241 ggaagcttac tttagcgatt atttttgata aaaaggatga tgtttagatg aaaatttatt
    45301 ttgccgcacc aatgtttgct aaaagtgatt tgttatataa ccaacagtta gttaaagaaa
    45361 tcagagagct ctcttctgag ctttcgatct atcttcctca agaaaatgaa gcaatcaatg
    45421 acaaaactgc ttatgcagac agtcgaatga ttgctcttgc tgacacagaa aaagtgttgg
    45481 ctagtgattt aatgattgct ttactagatg ggctgacgat cgatgccggg gtagcatcgg
    45541 aaatcggtgt ggcttatgcg aaaggcatcc cagtgatcgg cttatatact gatactcgac
    45601 agcaaggcgg aacccacccg aaaaaaatcg ctgcactaca agaaacagct gaaaatcaat
    45661 ttcactacct gaacttatat acaataggtt tgatcaaatt gaacggaaaa gtggtttctt
    45721 cagaaattga gttgctttca gaagtaaaaa gatttttaga tggagggact ttcagtgatt
    45781 aaagaaatca aaaaggtgca aatagcttta cttgcttttg gtgtattcgt tgtctgctat
    45841 aatttttatg aatttatcac acaaaaatat tcgacttctc aaggaatcac atttatcgtg
    45901 gaatctttat tagggattgc attgattttc atgccacaag tcattttgaa agttttcaaa
    45961 cttaaaatac cagcagcaat cgttttgttt tactggtttt tcttgttcat ctctgtcttt
    46021 ttaggcacag ggatgcattt gatcagtatc atctcttttt gggacaaaat cttacatgcg
    46081 gtgagtccaa tggtcctgac agctcttggc tatggattga ttggttactt gatgaaagat
    46141 gctgagatat caaaaaccag tccttggttg ttcctcttat tcggctttgc gtttgctgga
    46201 ctttgcggcg tgttttggga gttctgggaa ttcttatgcg accagttctt aggcatgaat
    46261 cttcaacgtt ttgctgcttc tgatggcaca ctgtttgtcg gacgagcagc attgatggat
    46321 acgatgggcg acttgctgac aaatacgata ggtgctgcct taatgggatt gtttgcatgg
    46381 tctcaaagta aaaaagatga gcgttacttt gaaagttata aattagaaaa agtgaaaaat
    46441 acctaataaa agaggttgtg acagaagcgg gcagcttcaa gaaatttcgg cttatttttc
    46501 gaagaaacta cttctggaac actgtttatt tgaaaaaagg aggtctgaca ctagttgtgt
    46561 cagacccctt ttttctttta tagtctcaat ggaagattag acgatttctt ctagctcttc
    46621 tgttttgaaa tgaagttgtc catcaagtaa agtaatcgtt acttttgatt taggcataac
    46681 atgtcctgca acgatttctt tagctagtgg tgtttctact tccttagtga taaatcgttt
    46741 caatggtctt gcaccatatg ctggttcata agcattttca gcaatccacg ttttggcttc
    46801 atcactgatt gtcagcagga tttcttgatg ttccagacgt tgtgccaatt gcgccaccat
    46861 tttgtcaacg attcctttca cgttgtctag gctcaatggt gtaaataaaa tcgtgtcgtc
    46921 gattcggttc aagaattctg gtttgaagtt tccacgtaat agcgtgttga cttgttcagc
    46981 cactgcttct ggaatcgttc catcagcagt cacgccttcc agcagtaact gtgaaccgat
    47041 attgctggtc atgatcagaa ccgtattttt gaaatctact actcgccctt ttgaatcagt
    47101 caagcgtccg tcatctagga cttgtaacaa gatattgaag acatcgggat gcgctttttc
    47161 gatttcatcc aacaagacga ttgtataagg atttcgtctg acagcttccg ttaattgacc
    47221 accttcttcg taacctacat agcctggagg agctccgact agacgagaca cagcatgttt
    47281 ttccatatat tcactcatat cgatccgtac catatgatct tctgagtcaa acaagttctc
    47341 agctaacgct ttcgctaatt cggtttttcc gacaccagtt ggtcctagga aaaggaaaga
    47401 acctagagga cgatttgggt cttgcaatcc agcccgtgaa cgaattaccg cgtcactgac
    47461 cgcatcaact gcttcatctt gaccaatcac tcgtttatga agtgtttcat tcaatttaat
    47521 tagtttttct cgttcacctt ctacaagttt cgttacgggg atacccgtta atctgccaac
    47581 gacttgagca atctcatttt cggtaacgga ctcttgaacc atcttaatct cgctgtcttt
    47641 tgctttagct tctaattcct tcaattcttt ttctaattga ggtatcgtac catgacgcaa
    47701 tacagcagcg cgttctaaat catagttatt ttctgcatct tctaattcgt gttttgcttt
    47761 atcgatttca gcacgttttg ctgaaacaga attgacttct tctttttctg tttcccactg
    47821 catcttcatc gcatttgctt cttcacgaag ttcagctaat tcttcttgca aatttttcaa
    47881 gcgtttttta cttgcatcat ctgattcttt tttcagtgca gcttcttcga tttccaactg
    47941 catcaaacgt ctagtaactt gatctaattc cgttggcata gagttcattt ctacccgaat
    48001 cgttgcactg gcttcatcga tcaaatcgat tgctttgtcc ggtaagaacc gatcagtaat
    48061 ataacggtca gataaagtag ctgctgctac taatgcgttg tcatggatgt taacaccatg
    48121 gtggatctca aaacgttctt ttaatccacg cagaatactg atcgtatctt ccactgttgg
    48181 ttctttgact aatacctttt gaaagcgacg ttccaacgct ttgtcttttt ccatatattg
    48241 acggtactca tctaaagttg tggcaccgat caaatgtaat tcaccgcgag caagcattgg
    48301 tttcaacaga tttccggcat ccatacttcc ttctgttttt cctgccccta cgatattgtg
    48361 gatttcatcg atgaacaaga ggatacgtcc atcacttttt ttgacttctt tcaagactgc
    48421 ttttagtctt tcttcaaatt caccacggaa ttttgctcca gcgatcaatg cacccatatc
    48481 taatgaaaaa atcgttttat cttttaaatt ttcaggcaca tctttgcgta cgatacgttg
    48541 agcaagccct tcaacgattg ccgttttccc tacaccgggt tctcctatca gaacaggatt
    48601 attttttgtt tttcgtgaaa gaatacggat gacatcacgg atttcttcat cacgaccgat
    48661 gattggatcc atttttccgc ttttgacttg ctgtactaaa tcaacgccgt atttttctaa
    48721 tgctttgtat tgttcttctt ggttttgtga ggtcactctt tctcctcctc tcatctcttc
    48781 gatgttttta cgcaattctt tttcagataa tccgttttta ttcaaataaa cagttaacgg
    48841 ataatttttt aatttcatca acgctaaaat aacgacttcc gttgaaagaa actcgtcgcc
    48901 gaagctttca cgtatttggt ctgcttcatt caataatcga aagaaattct ggctgaggtt
    48961 ttgtccatat tggacgtttc caccagaaac aactggatac tcatccagta gacgatcaat
    49021 ttcatgttca aaagattcga catccaatcc ggcatccgta tagaaatttc gaccaaaatg
    49081 attagattgt aaaaagattt tccatacatg ggcaatgtcg atatcttgat gttttcgagt
    49141 gaccgcaatc tgttgagctt cagcaatcgc ttcttgaagc gtcgtagtca ttttctcaat
    49201 attcatacag gtataccctc ctaattaaag gtaagatcac ttatttcttt gatctttaca
    49261 aaaactatta tatcgctttg gtcaattttg gtcaaaatat acacatagtt ttttgacttt
    49321 ttttctcgac ttatataaat cttaaaaaaa caaaattgat tgtatgtata taaaaaaaga
    49381 gccaaacaaa cgtaaaatta tcttatccat aacgtttttc tttgatttga taaaaagggt
    49441 agatcttata aaagacctac ccttgatttt ctttattctc tttttttact tctattattt
    49501 cttctttgat cactcttcgc cgttcagcaa tcacatacac aaatccatag agaaatggca
    49561 agataaagga actgaaacga tacagtaaaa gcgaagaaac tgcgtcaact gttccaacaa
    49621 ctggtctgaa taataacaaa tagacaaatt caaaggaacc aataccagca ggcgtaggaa
    49681 taacccctga aagcactact gcaaagctga taaacgaaat ggtcagtaga aaatctattt
    49741 taggatggtt ttcaatcaga atcaaataag gaagtacata ccaaaataca agttttaaca
    49801 tattccatag ataaatccgc aatagcgcag tcctgtcttg gataatggtt tggaccgttt
    49861 cccttaaaga gtagatctgc tgattgcatg tatccaccca tttccgcaaa cgctcacttt
    49921 taaataactt attcgataag ctgacaaaaa atatttgaac attcaagctg acggacatcg
    49981 ccaataaaaa ggcaacaatc acgatcgtca aaaggatacc agcgatgata aaccagatca
    50041 ttgatggacc attagaataa aaaagagaga attggatgac caagccagct aatgcataag
    50101 tgatcactgc cagtttgtac ataatcatat ggagtgctgt cacccctgct ccctgggata
    50161 gcttcattcc ttttttgtta taaaaataaa tctccgagat cagcgttcct gtaccaaacg
    50221 aaacgattcg gtaaaacgca atatagctag aggtcaagaa cccatctttc gttgtaaaat
    50281 tcggcgcgaa aggttcagca atttctttga ttgagcgccc ttcagcgatt tggtaaactg
    50341 tccctaatat aacagccgct gccaaaacaa caacactggt agacagcaat tcggcaaaga
    50401 tatctgcaaa tgattgattg attaaataat aaataatggc aaaaatcaca atcagcaaaa
    50461 gcagattcag aatgatttta gttttcgttc ctttagacga catctcctgc tccttaatgg
    50521 aaaaaataaa tgagaatcaa atcaacaagt acacctgcag cgaatcctat agtaaacttc
    50581 tttttccgag ttttatgatg gaaaactttt cgagagatca atcctcccaa accaccgcct
    50641 aacaggctga caaatatcaa attattctca ggaacacgcc agccgctagt tctagcctga
    50701 tgtttatcat accccatcag acaaaataag taaaaattca caatgaaaag gtaaaaccac
    50761 aaaggattgt gtaacaatac atccattact cttcttccaa ctttctgtta actactattt
    50821 ctaagctttt tcttctttct ttggtgaata aactgttaat ccttttgata caacagataa
    50881 ttcaactgga agatcatctg tcgtgtctcc atccgttcga gtgcccacag atttttcctg
    50941 cgcttggatt ttcatgcgtt tggctgtgaa ataagtaatg ccggggatcg tatagaattt
    51001 gcctctcatg attctaggca aataaaaaat aaatcggaaa atatctaagg aaggtacgat
    51061 cgttacatga aattttccat catctagggt cgctgagtcg tcaaaatttg taaaaccgcc
    51121 cacggagtta gacatcgtga ctgtcaacag ttgggatttt cctttccaat gctcctcgtc
    51181 tgtttctaaa tccaaatgat attttttctt cttcagcaat aacttaaaaa acttcttgat
    51241 aaagatccac gagccgtaac gctgtttttc ttcttgactg atccaaactg ctgtatctgc
    51301 taagattccg attgtcagtg tagagataat gacttcatca ttgacttgac catagtctat
    51361 tttccgtaca ttgccttcca ggatcacgtc agcagcttct tcttttttga ggggaatctc
    51421 aagaacacgt gcaaggttgt tgacagttcc acctggaagt aatccgacat ggtattgaga
    51481 taatgttttt tggaacgctt ggacgatatg atgaatcgtg ccgtctccac ctataattac
    51541 taaagtatca gcttctatgg cttctgcttt cttgactaat tccgcatcat caatcgatgg
    51601 gccagtttca gctagttcta catataattc tggctgcttt tcttttacat aatcttgaaa
    51661 ccagcgtgct aatttctcac cctcatcttt ccctgaactt ttgttataaa aaactagcag
    51721 cttttccatt ctttacctcc tatttactct tcaaaaaaga gggcgggaca accaattcca
    51781 ttgtcctgcc ctttagcctt agacagatga cattccatca ttgggacgaa cgccttgtca
    51841 taagctgttc tttttcgatt ttaactcttt gtgttcaatt ctgcaatttt tacaatcacg
    51901 tctgttgctt tttccattac ttgtaaagaa acaaattcga atcgtccgtg catattttct
    51961 cctccggcaa atagatttgg cgtcggaata cccatgtaag agatttttga accatctgtt
    52021 cctccacgaa ctggttcgat cactggagag ataccaagct cgatcattgc ttgtttcgct
    52081 aattccacga tgctcatatc tttttcaatg atttctttca tgttgtaata ttgatcatac
    52141 atatctactt tgattcgttc ttgatcaaat cgctgattca actcttcttg gatatgtgtg
    52201 atttgtgctt tgcgtgcttc gaatttttca cgatcatgat cgcgaatgat ataactcata
    52261 gaagcttctt caggattccc atccaatgcc atcaaatgga agaaaccttc gtatccttcc
    52321 gttttttctg gaacttcatc ggctggaagc tgattatgaa aatcgattgc taactgtaga
    52381 gcattgatca ttgtatcttt tgccgtccct ggatgtacat ttttcccttg gatcgtaata
    52441 tttgcttgag cagcactaaa cgtttcatac tgcaactcac ctaaaggacc accatcgact
    52501 gtataagcaa aatctacatc gaattgctca acatcgaatt tatccgctcc gacaccgatt
    52561 tcttcatcag gaccaaaagc cactttgatg gtaccgtgag ggatagaagg attattgatc
    52621 aagatctcca ttgcagtcat gatttccgca atccctgatt tgtcatctgc tcccagtaat
    52681 gtactgccat ctgttgtgat caatgtgtgg ccttcataat ttttcaaatt tggaaaatct
    52741 tttgtattca atgtatagcg ttcttccgca tctagtacga tcgtagactc gccatcatag
    52801 ttttcaatga tttgtggatt gactcctact gcattaaaat ctgctgtatc catgtgggaa
    52861 ataaatccaa tggaacgcac ttcatgatca acattgcttg gtaatgttcc cataacgaat
    52921 ccgttatctt cattatagaa aacatcagct aagcctaact cttctaattc ttttttcaat
    52981 tcttgcgcaa aagcaacttg tgtctgagtc gatggagtcg ttttgcttgt tgcatcggag
    53041 cgtgtttctg ttttgacata tcgtaaaaaa cgtggcaaaa gattttcgta catagtattc
    53101 tctcctttta attaaattga aatgggtttg tatttgtttc agaaacgaaa aagttaatat
    53161 cccaattttc ttcttgtttc caagattcaa atttctcgat aaatcgttgc ttgcataacg
    53221 attcaatata atgaccggga tcaattgcaa tcaacccagc gctttgcata tcatgagccg
    53281 tatgataata gatatctccc gtgatataga catctgctct ttgggcgatt gcttgcggat
    53341 aaaatttttc accgcttcca ccgcaaatcg caatgcgctt cacagaggat tttgcatttt
    53401 ttggctggac tattctcagt ccatctagtt ggaacgcttc ttttacttgt tctacaaatg
    53461 cctctataga aatttcttgc ggcaattcac ctactcgtcc taacccaaac atttcgactg
    53521 gttcatctat agcgaaaaga tcataagcag gttcctcata aggatgagct gaccgcatgg
    53581 cttgaatcac ttgtttttcg atagtttccg gcaaaataac ctcaactttt gcctcttgaa
    53641 cttgttctgt cttccctact ttcccaatcg ctggttgtgc accagcttcc ggagtaaatc
    53701 gaccgtgtcc aatcgaggta aaacttgtac cggtataatc accttgtgta ccagcaccag
    53761 ctgcagctaa tacttcacgc attttctgtg catgatccac aggtacataa acggccagtt
    53821 ttttataatg aatctcatga gtctttacta aataactctc cacttcaata ccgagaagtt
    53881 cacaaaacca gtcattcagt ccatcccaaa taatatccat gttcgtgtgt gctgcataga
    53941 cggcaatatc atgtttcaac aaatctgcat acattttttc ttgtggctga tcagtaacca
    54001 gccgttttac aggacgaaag attggaggat gtttagcaat cagcaaatcg atttttttct
    54061 tgattgcttc ttcgaccact tctggacgaa cgtctaatgt catcattact cgttggattg
    54121 gcttatccaa tgttccgata tgcagtccga ctgggtctcc ttcttcagct agccactgag
    54181 gacaatagct ttcaaacttt tggatgaaag tgcgtccgct gattgtcatg gctttaaaac
    54241 ctcctcaatc atttggattt cctgctcgat ttgtttttga cgtgcctctt gattagctgc
    54301 ttgtttgagc tgttcacgga tagcttttct ttgttgaagc tctcttgtcc attttgattt
    54361 gaagatctca cttttctctt caagcagaaa tggcccgaac atcagctcca tttcattgta
    54421 agtaactggt ttttgttctt tttcggctac aatgatttca tatatttttt tattttcttc
    54481 aagtattttt tctgattgga ttttatactg attttcttgc agccaagatc gtaatgtacg
    54541 ctctccgatg tttggctgaa ggatcaaacg ttcttgtccg cttaagcgtt gattcaactt
    54601 gccagcttct aggatatctc ggatcagtga acctcccata cccgcaatcg tgacagctgt
    54661 aataccatct tgtggctgga tagcatctag accatttgca aggcgtacgg tgatacggtc
    54721 ttctaatcca tcttttttga cttgcttacg tgctgattca taaggacctt gaacgacttc
    54781 accagcaact gcatattcga tttttcctct taatatcaat gcaacgggta agtaagcgtg
    54841 atctgatcca atgtctgcta aacgagcgga ttcagggaca aagtcaccca ctgcggctaa
    54901 gcgctgagat aaatctaaat ggttcatagc ttcactcctt ctagataatt atagcatttt
    54961 ccaaataaaa taaggagact aggaataaaa aaattcccat atctccttaa aaaatatacc
    55021 ttttcgttct ataaaacaac attttcttat ttttgaaaaa atgttattgg ttatttgttc
    55081 aatgaaacat caataatcga atctccagga acttgcggtg ggatagacat cgtcatgatc
    55141 ggcatctcaa ctaatgtaaa ttggactttg tcaccgattt tcaattcatt tatgtccacg
    55201 cttatttttt cagcagtagc atttaagatc actccgtcat tgctgaatga cgttcccata
    55261 ttcgctggat cttctttggc catgacatcg acgagtttca actgaagcgt ttcttcttct
    55321 gcttctactt gagtgatact ttcaatcgtt ccgacaaata tgctttgtct ctccgttttc
    55381 gtactgcttg tttttactgt ttgacccgca tcaatcgaat caacagaacc tgtgttgcta
    55441 tctgcgctac cgcagcctgc taaaagaaaa aatagaccaa ctacagatat actagaaaaa
    55501 atagccctca taaaaaatcc tccgtttaag tttctgcatt acttgttgcg gtgattgtct
    55561 ccggtcattt ctgtccatat aatgattcgc tgacctgctt ttactactag gtcaaatgtc
    55621 agttggactt gtcaactgtt caaccattgc gtcggttgcg accagtcatt tcgatttctt
    55681 ttgtcaaata acgtatgcgt tccattatcc gtttcgcttt tagcggctct tcatctccac
    55741 gttgagtgac ggacaaatgc tgttccaatt catccattgt aaaattcgat gagaagaagg
    55801 ttggtaactg ttcctgcatc cgatattgaa ggataacacc gaatacttcg tctcgtatcc
    55861 aactactcat cgcatctgca ccaatgtcat cgatcatcaa aataggtgaa cgtttcactg
    55921 catctaactt ttctgccact tgatcttttc cgattgcctg tttcatctca acggcaaatg
    55981 tcggaaaatg gactagcgtc gtggtgaatc cagcaatcgc taattctctc gctgttgctc
    56041 ctaataaata tgtttttcca ataccaaaac ttcctactag atacagtcct ttatgatacc
    56101 cttttggatc agcagtatat ttctctacaa aatctagggc ttctaagctc gcatatcctc
    56161 gtccatctgt acgttcaaag ttttcaaaag aagccgattg aatatctttt ggcatatcca
    56221 ttgcttttac tcggcttctg atttcttctt gtctttgccg tgctaacaat gcttccgttg
    56281 gtacataagt gacatccaca taatgaaaat tcaacgtcag cttcggctca tagccgggag
    56341 cgatcatcgt tggatcgttt ttttgatatt tacgcttttc ttgcacgaat tcatacaatt
    56401 tagcgtaact tttttcaata tccgcttgtg tcaattcttc ttggtgtgcg cttagaaaag
    56461 cttgaacatc cgcatcattt aagacttcag cgatcatttc agcatacttt tcttgataat
    56521 tgcgtttgct gatgatcctc ttcaattctt tgcctacgtc ttccactaga tatcaccctc
    56581 cttttttgcc aggtattctt taaaacggcg gtcaatctct gcttttttct cgctgctgat
    56641 ctgtttttca tcttttggct ggttgaccca atcaggaagc ttttcccgtc gaacatttcg
    56701 cttgccagtt tgataatttg tttgcttctt ttgttttgat tgttttgctt gattacttat
    56761 ttgccgaatg tgctggatcg cttcttctgc tgtggcgatt ttcttttgtg cccattcatt
    56821 tgccactgta tttacataac tggcatttaa gctagcatta ttcttgatca ctagtacata
    56881 attcagcaaa acattgatca cgctactgga aagacctgat tgagaaacaa gatcttgcag
    56941 taaccagttt tcttgtttag aaacatatcc gcctttttct gacttgattg cttccagata
    57001 tttcatcggt ggaatgctct gtgcttcttt tatcagctga agcgctgcgt ttgtcaaatg
    57061 ttcgtttgac gagggttcat tactgttttc agaaaccgca actgtttctt ttgtttgctt
    57121 caccggacgt ctctgcattg ccaaaatggc tttttgcagt tctttttcag atactttttg
    57181 ttcactaaag tcataagatt gactgatcag ttcgccaagt gtcaattcat cgaaaccgta
    57241 aagctgatga tacatgtcga cttgtttgat gattggatca tcaagacttg caatatgttt
    57301 ttttgccagc caatcattca aaaactgcca atcgaatgtc gaatctgctt caagtaatgc
    57361 tactgattca gattcttcaa aagatttatt cagcgattcg atgttttctt gattagcagc
    57421 gtacactgct tcattgaagc gataaacatc taaaaatttt tttgtgattt tttgatatcc
    57481 ctctgctgta tgccttttag gttcaaagaa ttgggtaagc ttctggaatc gtcgttcacc
    57541 aactttttcc agaagaagaa aagccagaac agagtctttg aaaaatgctt taggaatgag
    57601 tggttctaac agctcataaa taaaacgagg tcccagatca ctatcttctt taaaataaac
    57661 atccagtaag ccgattcctt ctagcttttt tcttgcatca taaaattgcg ggataccgca
    57721 gcctagacta ctaagcagat ctgcatgcat cccttcagga cctttgccag attcttgaga
    57781 gatttctgat aacagggtga aatagatact caaggcttct ccgccgatta gtggctgata
    57841 aagatacagc aaggcgttct tgctgtcttc tctcagcgga gccgcttttc ttccttggta
    57901 aatatttttt ggttgaagct ctttccaagc attttccaag gaaatgcctc ctttttgttt
    57961 cttaattgtt ttctttatta ttctctttat tattttcttt tgctttatcc acgatatctt
    58021 gcaactcttt taagaaaaca gacatatctt tgaactggcg ataaacactt gcaaaacgaa
    58081 tataagcgat ttcgtctaaa tcaactaaat cttccatcac atattcacca attagattcg
    58141 tcgatacttc gttttctccg agactgcgta cgcggttttc gacatggttg acgatttgtt
    58201 ccatttgttc catagctaca ggtctttttt ctgctgaacg gatgagtcct cttaaaattt
    58261 tatcgcgatt gaactcttcg cgttccccgt ttttcttgat tactaataac ggagcagctt
    58321 caattcgttc aaatgtagtg aatcggaacg aacaattctc acattcgcga cggcgacgga
    58381 tcgcacgtcc atcatctgct tgacggctgt cgattactcg tgaattatta tgttgacatc
    58441 tgggacatct cattttgtct cacctcttgc atcgcatttt atataaagga tgtgtctttc
    58501 tttacaacta gttgtattat aacacgaaac aaatcacaaa cgcgactgtc ctacccaaaa
    58561 aacacggata taagaacaat tttttctact ttcatgcgta attgtgtccc ctgtccttac
    58621 taaatttcaa ttttccaata ctgatcacct tgattactga gaaataactt acacaaaagc
    58681 tgatttagtc atctagcaca aaaaacttga agcagatgaa caccttctca tctgcttcaa
    58741 gtcacatctc gattttttac tattttataa cttctcttcg attcctgtat agtgtttcat
    58801 tcgctgttcc tcgatttttt gctcatcggg aatgtggtca ctttctggga gcggtgtttt
    58861 actacgatca ggtgtcggat tttttcgttc ttgagtcaaa tcttgccgct tcgcttcttc
    58921 ttctgcctgc tggtcttcta tttcatcgat tcttgcttgg tactgctttt ctttttcttc
    58981 tgctgtttct gattgcgtac gaccttcatc gattagattt tctttattga gcatctcgcc
    59041 gattttttct tttgcgttac ctacgacttt atccttttga ttttttaatg atcccatcgc
    59101 tatccctcac tttctatata ctcaggatag caaaattgac acagatactc aaagaaagcg
    59161 attagcaaaa agccaatttt cgatttgtcc cacaagtatt tcttttgttc cattgttgtc
    59221 gaacaaaata tccgcacgct gctttttttc ttcgattgga agttgactac taattctctg
    59281 ctgcgcttgt tctagcgtca gttgattacg tttcatcaaa cgctgaagct gagtttctgg
    59341 cgtagtataa acgacagcta ctgcatccat atagtggtca taatgacctt cgtagagcaa
    59401 tgggatatct acaattacta aaggatgatt ggaacttaga gaggcgattt gatcttttat
    59461 cgcttttcgg ataaacggat ctaatagctg atcaagtttt tgccgttttt cctttgatgg
    59521 aaaaacaatc atcccaagtt tccctcgatc tagttgccca tcaggctgca atacctcttg
    59581 gccaaacact gatacaattt tcctcagagc aagcgttcca ggctcaacag tttctctggc
    59641 aatcacatcg ccatcgatca aaggaaatcc atgagactga aagactttgg ctgccgtact
    59701 tttaccggta gctattccac ccgtcaaacc taaaacaaat cctactttgc gatttttagc
    59761 ctttgacaat tcggacaata gtgcgtcccc ctttgtgcca cttttgtttt cacgatcggc
    59821 gttccacaac gtatacatgg tttgccagtt tgctgataga catgcagtgc tacttgaaat
    59881 ccgcctgatt ctcctaatgc attcaaataa ctgcgaatcg tggtacctcc ggcttctatt
    59941 gcacgatcca aaacatcaat gatcgataga cgtaattgtt ctacctcttt ggggcgcagt
    60001 gtgttcgccg gttgttctgg atggatcttc gcttcccata atgcttcatc tacataaata
    60061 ttacctagac cagtcactag acgttggtct agcagcaatg gcttgattgc tttgtgggac
    60121 tttttcagtc catttgcgaa atctgctaat aagaagctat ctggcaaagg ctcaggtccc
    60181 aatttcatga ttcctcggta agttgcactt gctccttttt cgacgattgt catacgacca
    60241 aattttcgta catcgttgta gcgaagttgg cttccatcct caaaaaagaa aagtacatgc
    60301 gtatgtttat cgattggtgt attttctttc gtaaattgat attttccttc cattcgcaag
    60361 tgtgagatca gttcacaatg atcgagatga aaaattaaaa acttccctct acgtccaatg
    60421 gattggatgg tttcgccaac aagagaagcc tcaaagatgg gtgtctctgg ttgctcgatg
    60481 atcttgggcc acaagacctg aactttttga atcgtttttc ctactactaa ccgttccaat
    60541 cctttacgaa cggtttctac ttctggtaat tctggcattt tttcttttca ccttcattat
    60601 attgctctgt cttaaacaat gtttattttg cttcgtacca cgtttttccc caactgctgt
    60661 cagtgatcag tggtacatga agagaaacag cttgttccat aacttccttc accaatttat
    60721 ctaatgattc aagttctttt tccggtacct cgaagacaag ttcatcatga acttgtaaca
    60781 gcatcgtagc ttgaagacct gtttctttta atcgtttatc caattcgatc atcgcgattt
    60841 tcaaaatatc agcagcactt ccttggattg gtgtattgat agctgttcgt tccgcaaaag
    60901 aacggatatt gtaatttctt gaattgatgt ctggcagata tcgacggcgg tgatacagcg
    60961 tttcgacgta gccttggtct tttgcttcgc gtacgattct ttccatatat tctttgacac
    61021 ctgggtattt ttcaaagtac gtatcgatgt attgctgtgc tgcttttcgg ctgattccta
    61081 gattttgtga aagtccgtaa tcagagatgc catacacgat accaaaattc actgcttttg
    61141 cctgcctacg catatttggt gtgacatcct ctgccttttc gattccaaat acacgcatcg
    61201 ctgtgctggc atggatatct tgaccttcta ggaaggcagc tttcaaatgt tcatcatttg
    61261 agatatgcgc aagtacgcgt aactcgattt gtgaatagtc agaagaataa atcagccagt
    61321 ctttttctct aggaacaaat gcctgacgta tctttctccc ttcatctaat cgaatcggga
    61381 tgttctgcaa attaggatca acagagctta atcgtcctgt ttgggtcaat gtctggacat
    61441 aacgggtatg cacttttcca tctgattgga tcactttcag cagtccctcg acataggtag
    61501 attgaatttt tgcaatttga cgataggtga ggatatcctc tacgattggt gcttgttcac
    61561 gaagctgttc cagtacatct acggctgtgg aatatccagt ttttgttttt ttgataacgg
    61621 gtaatcccat tttttcaaat aagatcactc ctaattgctt tggtgaattc aaattgaatt
    61681 cttcgccggc ttcttcgtag attttttgtt cgatctctct taagcgagca gagaactcgc
    61741 ccttcatttc ttgtaagcga gtcgcatcta ctttgatccc gcttatttcc atctctgcca
    61801 aaatgataga caatggcagt tccattttcc ggaaaagatc ttcttggttt ttttctgcca
    61861 gttcttggct caattttggt gtcaacgcat tgattgcagc tacttttcgc gctaaatgag
    61921 caaaaaagag ttcctcttct tctggtaaac cttttttcgc ccctttgccg tatacagctt
    61981 catctgattg gatatcgttg tatccgtaat gagcagctat tccttcgata tctgtgcttt
    62041 tttcattcgt atcgagaagg tatgcaccta gcaagacatc aaaatcgatt cctttcgttt
    62101 ttccagtata gcggtttaaa gcaacataag ttcgtttcgc atcatatact tttttcagcc
    62161 gagtcggatc agtgatccaa gaatggaacg ctcggttttc gaatacagat agatcgttcg
    62221 tgacataaat tttcttttct gtcccccacg ctactccgac gatttcctct gtatgataat
    62281 tgtcgcccat catttccacg tacaacgcca tatccgtagt aaacatttcc tctttgaatt
    62341 catgaacgac ttcaaacaaa atatcatcca tttttacttc ttcttctacg atattcaatt
    62401 tagaaaggaa ctgattgaaa tccatttctt tataaaacgg aacgagttta tcgagatttt
    62461 tcccttcata cttcaatgag tcgatagaga catccacagg cgctgaagta ttgatggtcg
    62521 ccaatttttt cgataagaaa gcctgctctt tgtcatttat cagattttct ttcattttgc
    62581 tttgcttcat ttcatcgata tgttcataaa ttccttcgac tgagccatat tgtttcaata
    62641 gcttgatagc tgttttttca ccgattttag tcacaccggg gatattgtca gaggcatctc
    62701 cggctagtcc cttcatgtca ataatctgtt ttggcgtcaa cccatcatat ttttccgcta
    62761 catgttctgg tgtatagctt tcgatatcgc tgacaccttt tacagtgata tccactttta
    62821 cttctttact agctaattga gtcaaatccc gatcacctga aagcacgacg acatcaaatt
    62881 gatctttgtc tactttttct gccaaagtac cgataatatc atctgcttca taattgggca
    62941 attcgtaata ctgtacccct agtcctgtca acaaatcacg tatatatggc atttgttctt
    63001 tgaattcgcc aggtgttttg gatcttccac ctttgtactc cgcatacatt tctgtccgaa
    63061 aagtcgtttt tcctgcatcg aacgcaacca gaacatgagt cggttgctct ttttgcataa
    63121 cattttcaaa catcgtattg aatgcataga ttgcatttgt atgaagaccg tttctatttt
    63181 tgaaacgttc aagagaattg tgaagcgcaa agaatgcgcg aaaagccaca ctgttcccat
    63241 caacgagtaa taatttattt tttgtcatct tcttaactcc ttactgtaaa gttcttattc
    63301 attctaacaa agaatcctgc ggaatgcgaa aagaaaaagc gggaccatac caaataattc
    63361 ggtatagccc caactgatga atttttttct tttttagttg ttccgtccaa agattctcag
    63421 aaacgccaag aacaagttga taaagtccaa atacagcttc aatgccatga atacagcaat
    63481 tccactatgt gctgttccgt ttgtctggac atagatatta cggatgcttt ggttatcata
    63541 agctgtaatc cctgcaaaga tgacgactaa cagtagggag atgaacagct caactacgct
    63601 acttctcaaa atgaagatgt tcaagactag tgcaatgatc actccgatta aagctgcctg
    63661 tcctgctctt ccaaccccgc taagatctct ttttgttgtt actccgacaa cagacatccc
    63721 catgaacatc aaagtagctg tcaagaaagc ttgcgttact gttccaatat cgtagtatgc
    63781 aagagtaacc gctaatgtta tgccattcaa aagagagtag gcaataaatc caccgattgc
    63841 taagctcgga ttcttttgtg cttttacgcc tagaaccatt actaaaatca gttcaacgat
    63901 ccacagaccc acaaagccta gtggaaaatt attgataaaa gttgctaatt gcaaaggaaa
    63961 tacccaaaga gccaagtaag aagtgactgc actaagggca agtcccatac ctaaaaatcc
    64021 ataaactttt gcataaaaac gattgatacc tgacattaat gtacttggat tattcattca
    64081 attcaccttc actttcgatt gctatttcca attcaggaac ttgtggcggt tggaccataa
    64141 cgacagcatc caacacacga tcttcgttcg tattctccgc ttctacagaa atcgtcacca
    64201 catccttcat cttatctact tttatgacct caaactgaaa agtcagtgtt tcataatgga
    64261 aaaccggttc aataaaattg acagaaaaat tgacaacatg tgatcctggt ccaggaagat
    64321 gctttgaaat tgcacttgtg atgatcccca tcagcatcac tgatggaacg attggctgtt
    64381 gatattctgt tttttgtgca aagtcatgtt ggatatacaa aggatttgca tcattcgtca
    64441 atcccaaata cagcaaaagc tggttatctt caatagattc tgttaaggat aacgagtcgc
    64501 cttcctcgat ttcatttatt gtttttccta gttttctcgg cttacctatg ttcaagatag
    64561 aaaccctcca gtttattttt aatcatacat tattgtacca aaagtagcgt aaaaggcaag
    64621 ttctgctccc ttgatttaac agattcctat agtttacaga ggtcgtgaaa aagctgtcct
    64681 gcatcaaata ataagaacag ccaagaaaaa acagcttctc atgtttttga tttgattggg
    64741 cttattaccg cagatgcaag cttttgaaca ccgtttatca taagttgcaa aaaagggggc
    64801 ttggcaagat ttttgccact ccccctcttt ctatttttat aaggacactg cagtaattgt
    64861 attacagtac atttttcgtc gtgctgctta aaagtttttc atacgcttgt tcaaattttt
    64921 gaacatctcc agcacccatg aagattacta ctgcatcatg ataatctaac aatggagata
    64981 cattattttc tttgatgact tctccacctt ttttgatttt cgctccaagg tcttcaatct
    65041 taacgtttcc ttgttcttcc cgagccgaac caaaaatatc gcataagtaa actttatcag
    65101 ctaaatcaag tgcttctgca aattcatcca tcaatgcaat cgtacgcgta aacgtatggg
    65161 gttggaaaac tgcaattatt tctttatctg gatatttttg gcgtgcgcca tcaatcgttg
    65221 ctttgatctc tgcagggtga tgcgcataat catcgactac ggtcatgtct gctacgatct
    65281 tttcactgaa tcgacgtttt actcctggga aagtcagcat ttcttctgca acttctttca
    65341 gatccagttt ttcgaaataa gctactgcaa taacacctaa agcatttaag atattgtgtt
    65401 taccgaatgc tggtacagtg aagtgcccaa caaattcatc cccatgatag acatcaaaag
    65461 cagaaccaga agttgtgcgt tcgatgtttc gtgcttggat atcatcgttt tctgtgacac
    65521 cataataata aatcggcaca tttgctttta atttgcgtaa ataagcatcg tcaccatacg
    65581 caaaaatagc ttttttcact tgtccagcca ttgtttggaa agctgtaaat acatcgtcga
    65641 tacttgtata ataatccgga tgatcaaaat cgatattggt catgattgca tagtctggtg
    65701 aataggcaag gaaatgacgg cgatattcac aggcctcaaa tgaaaagaat tccgcttgcg
    65761 gatcaccatg tcctgttcca tctccaatta gataacttgt tggacggaca ccactcaata
    65821 catgagagag cagtccagtc gtacttgttt ttccgtgaga gcctgttaca gcgatactcg
    65881 tataattttg gataaaatga ccaataaagt cgtgatagcg aatcacttcc aaacctaatt
    65941 cttttgctcg ctggatttct tcatgtgaat caggaaatgc atttcctgca atgatcgtca
    66001 tccctggttt tacattatct gcgttaaatg gcagaatcgt gatatttgct ttttctaaat
    66061 ctctttgtgt aaagaaatat ttttcaatgt ctgatccttg gacatttaga ccttgttcat
    66121 gtaaaacaag agccaaggag ctcattcctg aacctttgat accaacaaaa tgatacaatt
    66181 tattttgatt ttccatttga atcctcttct cttatctagc gtccattctt ttagctatta
    66241 cattcaacac gctcctcatt atcttataaa cgattgaaaa tttcaaccaa atttatttgg
    66301 aaaggaggat tcgcttgcta aaatggtcaa gtacgagaaa aatccgtttt tattgactct
    66361 ttaattctgg tgtaagaaga ccaaacttcc tctgatatat caatgtgagg aataaaatct
    66421 tttcctttcg gaccatagcc gttctcatcg tcatggagac gttccatttc tcctaatact
    66481 agcgcatgga aatattcctt aggccattga tgattcagtt cttttgctgt aaagatgatc
    66541 tcttcagaaa agtgaaggat ttccggcaca aatggcacag cattcgcgaa aatagtttgt
    66601 tcattttgcc atactttctc aaaagttgct cgagtcctgc gttgcagtgt cggatcattc
    66661 atcaataaga ttttttctgg tacagctcct aaggaatgaa gaatctccag tgaaaaagtg
    66721 gcgttctctc cagaatttgt agacttcgat tctatcagaa aagccttatc gggcaaatca
    66781 tagacttctt ttagatactg acgacagatt tcactttcac tcatgccatc ttcaaaatga
    66841 aatccttgct tttcaaaatt ctcataaagg attctagtcg catgccccac ccctccgacc
    66901 aaaaacaatt gatctacttg gccatttaaa tacatttgtg ctgctttatc agctaagata
    66961 ggaagacagt ttccagctaa aataagtgta ggtgccgtca gttcctcaaa atttccctct
    67021 tcaggtgcgg ctaaaaaatt taatagctga ttccagtctt ttagattcct ctgctcactt
    67081 ttttccaaag gccttctatc ctttcatctg attttttgtg cttcattaaa gtcttctctt
    67141 tttgtctcaa tccatttgat ttctccatga catcttccac aacgatattt gcgtacatcg
    67201 attcttctct tgcggcagat cgtggcagaa caatcttggc atcgataata ctccatcttt
    67261 atctgtttct tcgtcaaggc tggtgcatag cgcaaccctc ctgttttttt caaaagatac
    67321 ttgaaatcca tatctttatg ctgataccct cttccagcta gatgaagatg ataatggcaa
    67381 agttcatgtt tgatgattcc tgcaatcgtc tcttcgtctg actcttcaaa tagcttcgca
    67441 ttaaaatcta aatgatgatc gtttaaatga tagcgcccgc cagttgtacg taagcgtgga
    67501 ttgaacattg cttggtgctt gaaaggaagc tggaagcttt ttaatgatat ctcttctact
    67561 aactgctgca gttcttcttg tgtcaatttt tgttactcct cattgttact cttcataagg
    67621 ggaaagcatc gtcaaactga ttctgccttt tttcgtatcg acttgttcta tccaaactgt
    67681 tacgatatct cctactgaca cgacatctgt aggatgcttg acgaattttt tgctgagttt
    67741 cgatatatgg acaagtccgt cttgcttcac accgatatca acaaatgcgc cgaagtcaat
    67801 gacattacgg accgttccag tcaattccat ccctggtttc aagtcttcca tactcaatac
    67861 atctgtacgt aataagggcg ctggcatctc atcacgcata tctcgtccag gttgtgtcag
    67921 cccagctaaa atatcggcta acgtttcttc tccgactgat aggctttcag ccagtttctc
    67981 tacggataat cgtgtcaatt tttctactgc ttcttctgta ccaagctctt tttctgaaag
    68041 ttggactgaa gttaatattt cttttgcaat agaataactt tcgggatgga tacctgtgtt
    68101 gtccaaaatg tttttcccac cagggactcg caagaatccg attgcttgtt catatgcttt
    68161 tggtcctaag cgtggcactt ttttcaattg agttcgtgcg gtgaattctc cgttttcttc
    68221 acgataaatg acaatgtttt gggcagttgt tttattcaat cctgatatat gctgcaagag
    68281 ctgcgggctt gctgtattca catccacacc tacttggtta accgctgttt ccacaacaaa
    68341 atccaattgt tcagccaaac gtttttgaga aacgtcatgt tggtactggc ctactccgac
    68401 agctttagga tcgattttga ctaattcagc taaaggatct tgcaggcgac gggcaataga
    68461 aacagcactt ctttcttcaa cttgtagatg cggaaattct tttcgtgcaa tttcgctagc
    68521 ggagtaaaca gaagcgcctg cttcgttgac gatagcgtaa taggttttat ggtcggcgga
    68581 tttcaactgc tcagccacaa aaagttcaga ttcccgactg gctgtaccat ttccgatagc
    68641 taccatatct acttgatatt tattaattaa ttggataaaa gctggtccag cagcttctct
    68701 ttttgcttgc gctgcaggtt tatgaggata aataacttcg attgctaaaa ctttccctgt
    68761 cgcatctacg actgctagtt tacatccagt acgataagcc ggatcaaatc ccaggacaac
    68821 tttacctttt agtggcggtt gcaacaaaag attacggagg ttttcgccga aaatagcgat
    68881 tgcttgctca tctgcctttt cagtcaattc attgcgcagt tctcgctcga ttgctggctg
    68941 aataaagcgt ttataactat cctgataggc ttctctgacg aatgaagaag aaggagaggc
    69001 aggatttttg actatttggc gatccaagta agctaaaatt gctgcttcat ctacttgaaa
    69061 gaatactttc agaatatctt ctttttctcc acgattagta gctaatattc gatgagaaac
    69121 cattttatgg acgggttctg caaagtcata atacatttca tatacgcctt tttcatctgc
    69181 ttgctcatcc ttgacattgc tgacatacat acctttgtta tacgtataag accgaatcca
    69241 agtccgaaat tttgcattat cgctgacttg ttcagcaata atctcatgag caccgtgcaa
    69301 cgcttcttcg gcagaagaga cttctttttc tttgtcaata tatttttctg cttcagattg
    69361 cacctctcca tctgtaagct gcatcagcca cttcgcgagt ggttcaagtc ctttttcttt
    69421 agcaatcgtt gcttttgtcc gtcgtttttg tttgtatgga cgatacaaat cttccacttg
    69481 ttgcattttc acggcttggg tgatttctgc ttctaactcg ggagtcagtt tcccttgttc
    69541 atcgattagc cgaatcactt cagttttacg tttttctaag ttttcaagat aggcataccg
    69601 ctcttcgatt tcacgaatct ggacttcatc aagacttcca gtcatttctt ttcggtaacg
    69661 cgcgataaaa ggaacagtgt ttccttcatt taacaaatta agtacagtag tcaactgctt
    69721 aggacgatag tctgaaagtt ctttttgtac aagctgaatg atcgtctggt tcaaattttc
    69781 cgtcattaaa atgcctcttt tctgctaaaa attgcttctt tattctatca tactttttat
    69841 cctgggttaa actactgaaa gaaacaatat tctctataaa aataaaaaga gccgcaacaa
    69901 agtcacagct ccttcattct ttccattagt cagattccat gccaaggtcc ttcgttgttc
    69961 tgatattcaa tatcttgaag aataccttcc ttgtcaaaaa caaccccatg caatgaccct
    70021 ccgaatacag ccccaccgtc cattccgatt ttatgatcct gaatccagag gtctgttgtc
    70081 tgcatatctc catggagcat tggtgtaatc gtatgtccaa atacaattgt cttacctgta
    70141 ttgtttttcc cttcatgaaa tggttccctt atccaaataa agtctcttag acttgtctta
    70201 cgccagtctt tctttgtcaa atccacaccg gcatggacaa aaagataatt ttcccattca
    70261 taatacaagg ggcgctggac taaaaattta atcagttctg aatatctcga ttggatcatc
    70321 atcgagattt cagttgggga gtattcctcc gatgcccctg ggtgaatcag gctttcaaac
    70381 gtttcttttc caccatttct catataaggc gcataccagt cctccggtga ttgcagaaat
    70441 tgcaaaaaat attcttcatg gtttccccgg aggtaaatgg ctcctgtttc ttcgactaat
    70501 tgctttccta ataaaaaaca ttcttttgta cgaggacctc gatcattgag atcccctaat
    70561 aaaacaagct gatggatctt tggatcataa tagtgaataa gtttttcaaa aagctcatgt
    70621 ttgccatgga catccccaat agcataaatc aatgatttca tgtggatcac catcctcaac
    70681 gctagtatag cgcatattgg aaatggtcga caatcaaaac tattgttttc tatttaatta
    70741 aagctgtaaa ccgcagttct gcttcttctg gagagattcc atggtcttga acatagcgat
    70801 tgcgcggatg tttcgcacac tctatgctac atgctcctaa atgtttttct tcattttcaa
    70861 tggaagcaag aatctgacga ttgcactcag gattgctaca attgatatat cgttcacatg
    70921 gtgttccgtc aaaccagtcg cgaccgacga tttgtttatc tacttggttg atatcgacag
    70981 cgatccgttc atcaaacaca tacatcttgc cgtcccataa ttctccttgg acttcaggat
    71041 ccttaccata agtagcaata ccgccatgca gctgtccgac atctttgaat ccttcacgga
    71101 caagccaacc agaaaacttc tcgcaacgaa tgccaccagt acagtaggtc actacccgtt
    71161 tttccataaa ttcttctttg tgctcgcgga tccagtctgg aagctctcga aaactgcgta
    71221 tatctgggcg tacagcccct cgaaaatgtc ccaagtcata ttcataatca ttccgtgcat
    71281 caatcaccac tgtctgttca tctaaaatag cttctttaaa ttcttgcggt gaaagatagg
    71341 cacctgtcac ttggtttgga tcaatgtcct cttctagctt taaacttaca agctctggac
    71401 ggtatcttac aaacatcttc ttaaaggcat gtccttcgct tggatcgatc ttgaacatcg
    71461 tatcagcaaa tcgaggatca gctttcattg ctttcatata ggcagtcgtg ttttcaacgg
    71521 ttccagacac agtaccattt attccttctt cagctactag aatacgacct ttaagatcca
    71581 aagatttgca aaaggctaaa tgatttttgg caaattcctc tggttcttcg atttttgtat
    71641 aattgtaata aagcagtact tgataatcca tgtatttctt ccttccttct tttcgttcct
    71701 aaacagtata aagaaggcgt tcgcatttga cgatttattt gcttgtgtat aattacgcat
    71761 ggaaagtttt ttgtttacat aatattatta tttgaattaa aaagacttcg ctttcctgtt
    71821 tcgcacagaa aaacaaagtc tttatttcta ttatttacct gtaaccgcaa agtagttgcc
    71881 atcgggatca gcaaagttaa agacatattc ttctccaagc tgtaccatat cacctaatgt
    71941 gactgattgc gtctgcattt ttttatatag tccaaagata tctttactgt aaaacatcaa
    72001 agatggggca ctagtagctg tgccaggtga atgggactca acaaatgctc gatcatataa
    72061 cagaaatgag gtttgactgt cctcaactcc tacttcaatt ataagcgtcc catccatttc
    72121 ttgacgatct ttttccgcaa agccaactgc tctccaaaac gttgcatttt tttctacatc
    72181 gtccacataa agcatgattt ttatttcatt cgtaaacatc t
//
LOCUS       DANJMI010000008        71212 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000008 DANJMI010000000
VERSION     DANJMI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.008"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            189..419
                     /locus_tag="PWU81_000645"
     CDS             189..419
                     /locus_tag="PWU81_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDL1109377.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            516..1817
                     /gene="murA"
                     /locus_tag="PWU81_000646"
     CDS             516..1817
                     /gene="murA"
                     /locus_tag="PWU81_000646"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL1109378.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1830..2006
                     /locus_tag="PWU81_000647"
     CDS             1830..2006
                     /locus_tag="PWU81_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL1109379.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2141..2437
                     /gene="yidD"
                     /locus_tag="PWU81_000648"
     CDS             2141..2437
                     /gene="yidD"
                     /locus_tag="PWU81_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL1109380.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2622..3089
                     /locus_tag="PWU81_000649"
     CDS             2622..3089
                     /locus_tag="PWU81_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1109381.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHPYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3174..4058
                     /locus_tag="PWU81_000650"
     CDS             3174..4058
                     /locus_tag="PWU81_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL1109382.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4172..4408
                     /locus_tag="PWU81_000651"
     CDS             4172..4408
                     /locus_tag="PWU81_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HDL1109383.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4496..4576
                     /locus_tag="PWU81_000652"
     tRNA            4496..4576
                     /locus_tag="PWU81_000652"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4530..4532,aa:Tyr,seq:gta)
     gene            4583..4654
                     /locus_tag="PWU81_000653"
     tRNA            4583..4654
                     /locus_tag="PWU81_000653"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4615..4617,aa:Gln,seq:ttg)
     gene            4779..4949
                     /locus_tag="PWU81_000654"
     CDS             4779..4949
                     /locus_tag="PWU81_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109384.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5169..5876
                     /locus_tag="PWU81_000655"
     CDS             5169..5876
                     /locus_tag="PWU81_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1109385.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5935..6579)
                     /locus_tag="PWU81_000656"
     CDS             complement(5935..6579)
                     /locus_tag="PWU81_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL1109386.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6705..6992
                     /locus_tag="PWU81_000657"
     CDS             6705..6992
                     /locus_tag="PWU81_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109387.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7100..7420
                     /locus_tag="PWU81_000658"
     CDS             7100..7420
                     /locus_tag="PWU81_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1109388.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7501..8814
                     /locus_tag="PWU81_000659"
     CDS             7501..8814
                     /locus_tag="PWU81_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL1109389.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            8811..9530
                     /locus_tag="PWU81_000660"
     CDS             8811..9530
                     /locus_tag="PWU81_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDL1109390.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9658..10209
                     /gene="raiA"
                     /locus_tag="PWU81_000661"
     CDS             9658..10209
                     /gene="raiA"
                     /locus_tag="PWU81_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL1109391.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10431..12965
                     /gene="secA"
                     /locus_tag="PWU81_000662"
     CDS             10431..12965
                     /gene="secA"
                     /locus_tag="PWU81_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL1109392.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13091..14192
                     /gene="prfB"
                     /locus_tag="PWU81_000663"
     CDS             join(13091..13162,13164..14192)
                     /gene="prfB"
                     /locus_tag="PWU81_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL1109393.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14297..14983
                     /gene="ftsE"
                     /locus_tag="PWU81_000664"
     CDS             14297..14983
                     /gene="ftsE"
                     /locus_tag="PWU81_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL1109394.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14967..15860
                     /locus_tag="PWU81_000665"
     CDS             14967..15860
                     /locus_tag="PWU81_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1109395.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            16012..17460
                     /gene="cls"
                     /locus_tag="PWU81_000666"
     CDS             16012..17460
                     /gene="cls"
                     /locus_tag="PWU81_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL1109396.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17797..18654
                     /locus_tag="PWU81_000667"
     CDS             17797..18654
                     /locus_tag="PWU81_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL1109397.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18769..19689
                     /gene="pstC"
                     /locus_tag="PWU81_000668"
     CDS             18769..19689
                     /gene="pstC"
                     /locus_tag="PWU81_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL1109398.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19689..20573
                     /gene="pstA"
                     /locus_tag="PWU81_000669"
     CDS             19689..20573
                     /gene="pstA"
                     /locus_tag="PWU81_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL1109399.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20588..21394
                     /gene="pstB"
                     /locus_tag="PWU81_000670"
     CDS             20588..21394
                     /gene="pstB"
                     /locus_tag="PWU81_000670"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL1109400.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21410..22168
                     /locus_tag="PWU81_000671"
     CDS             21410..22168
                     /locus_tag="PWU81_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL1109401.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22181..22858
                     /gene="phoU"
                     /locus_tag="PWU81_000672"
     CDS             22181..22858
                     /gene="phoU"
                     /locus_tag="PWU81_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDL1109402.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23096..24127
                     /locus_tag="PWU81_000673"
     CDS             23096..24127
                     /locus_tag="PWU81_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL1109403.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24124..24891
                     /locus_tag="PWU81_000674"
     CDS             24124..24891
                     /locus_tag="PWU81_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL1109404.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24991..26559
                     /gene="liaX"
                     /locus_tag="PWU81_000675"
     CDS             24991..26559
                     /gene="liaX"
                     /locus_tag="PWU81_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HDL1109405.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26856..27176
                     /locus_tag="PWU81_000676"
     CDS             26856..27176
                     /locus_tag="PWU81_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HDL1109406.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPFARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27176..27526
                     /locus_tag="PWU81_000677"
     CDS             27176..27526
                     /locus_tag="PWU81_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL1109407.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27693..28631
                     /locus_tag="PWU81_000678"
     CDS             27693..28631
                     /locus_tag="PWU81_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDL1109408.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28646..29476
                     /locus_tag="PWU81_000679"
     CDS             28646..29476
                     /locus_tag="PWU81_000679"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL1109409.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29520..30545
                     /locus_tag="PWU81_000680"
     CDS             29520..30545
                     /locus_tag="PWU81_000680"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL1109410.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30560..31498
                     /gene="galU"
                     /locus_tag="PWU81_000681"
     CDS             30560..31498
                     /gene="galU"
                     /locus_tag="PWU81_000681"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL1109411.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31623..32063
                     /locus_tag="PWU81_000682"
     CDS             31623..32063
                     /locus_tag="PWU81_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDL1109412.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32066..32668
                     /locus_tag="PWU81_000683"
     CDS             32066..32668
                     /locus_tag="PWU81_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL1109413.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32727..33833)
                     /locus_tag="PWU81_000684"
     CDS             complement(32727..33833)
                     /locus_tag="PWU81_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL1109414.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34043..35044
                     /gene="ccpA"
                     /locus_tag="PWU81_000685"
     CDS             34043..35044
                     /gene="ccpA"
                     /locus_tag="PWU81_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDL1109415.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35381..37726)
                     /locus_tag="PWU81_000686"
     CDS             complement(35381..37726)
                     /locus_tag="PWU81_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL1109416.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            38005..38907
                     /locus_tag="PWU81_000687"
     CDS             38005..38907
                     /locus_tag="PWU81_000687"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL1109417.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            39120..40895
                     /locus_tag="PWU81_000688"
     CDS             39120..40895
                     /locus_tag="PWU81_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDL1109418.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40961..42547)
                     /locus_tag="PWU81_000689"
     CDS             complement(40961..42547)
                     /locus_tag="PWU81_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDL1109419.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            42798..43022
                     /locus_tag="PWU81_000690"
     CDS             42798..43022
                     /locus_tag="PWU81_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDL1109420.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43163..44914
                     /locus_tag="PWU81_000691"
     CDS             43163..44914
                     /locus_tag="PWU81_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1109421.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44914..46698
                     /locus_tag="PWU81_000692"
     CDS             44914..46698
                     /locus_tag="PWU81_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1109422.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            47024..47875
                     /locus_tag="PWU81_000693"
     CDS             47024..47875
                     /locus_tag="PWU81_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL1109423.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47971..49851
                     /locus_tag="PWU81_000694"
     CDS             47971..49851
                     /locus_tag="PWU81_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL1109424.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(49891..50793)
                     /locus_tag="PWU81_000695"
     CDS             complement(49891..50793)
                     /locus_tag="PWU81_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL1109425.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            51024..52427
                     /locus_tag="PWU81_000696"
     CDS             51024..52427
                     /locus_tag="PWU81_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL1109426.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52427..53860
                     /locus_tag="PWU81_000697"
     CDS             52427..53860
                     /locus_tag="PWU81_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL1109427.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            54005..54931
                     /gene="trxB"
                     /locus_tag="PWU81_000698"
     CDS             54005..54931
                     /gene="trxB"
                     /locus_tag="PWU81_000698"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL1109428.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55145..56872
                     /locus_tag="PWU81_000699"
     CDS             55145..56872
                     /locus_tag="PWU81_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL1109429.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56887..57237
                     /locus_tag="PWU81_000700"
     CDS             56887..57237
                     /locus_tag="PWU81_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL1109430.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(57329..58189)
                     /locus_tag="PWU81_000701"
     CDS             complement(57329..58189)
                     /locus_tag="PWU81_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDL1109431.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58281..58709
                     /locus_tag="PWU81_000702"
     CDS             58281..58709
                     /locus_tag="PWU81_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL1109432.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58871..59047
                     /locus_tag="PWU81_000703"
     CDS             58871..59047
                     /locus_tag="PWU81_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL1109433.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            59074..59520
                     /locus_tag="PWU81_000704"
     CDS             59074..59520
                     /locus_tag="PWU81_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDL1109434.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(59674..61053)
                     /gene="brnQ"
                     /locus_tag="PWU81_000705"
     CDS             complement(59674..61053)
                     /gene="brnQ"
                     /locus_tag="PWU81_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL1109435.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            61759..62730
                     /locus_tag="PWU81_000706"
     CDS             61759..62730
                     /locus_tag="PWU81_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL1109436.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            62754..64946
                     /locus_tag="PWU81_000707"
     CDS             62754..64946
                     /locus_tag="PWU81_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDL1109437.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            64963..65439
                     /gene="ybeY"
                     /locus_tag="PWU81_000708"
     CDS             64963..65439
                     /gene="ybeY"
                     /locus_tag="PWU81_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL1109438.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            65423..65818
                     /locus_tag="PWU81_000709"
     CDS             65423..65818
                     /locus_tag="PWU81_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HDL1109439.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            65833..66732
                     /gene="era"
                     /locus_tag="PWU81_000710"
     CDS             65833..66732
                     /gene="era"
                     /locus_tag="PWU81_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL1109440.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            66875..67687
                     /gene="recO"
                     /locus_tag="PWU81_000711"
     CDS             66875..67687
                     /gene="recO"
                     /locus_tag="PWU81_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL1109441.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            68071..68988
                     /gene="glyQ"
                     /locus_tag="PWU81_000712"
     CDS             68071..68988
                     /gene="glyQ"
                     /locus_tag="PWU81_000712"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL1109442.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            68990..71065
                     /locus_tag="PWU81_000713"
     CDS             68990..71065
                     /locus_tag="PWU81_000713"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL1109443.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        23286 a        12470 c        15245 g        20211 t
ORIGIN      
        1 atttggaata agggactgcg acaagacttt tgtctcagtc cctttttttg catattttta
       61 gcatttattt cctaaaaaat gtaaggggat tcctatattt ttaagtatac ttgtttttcc
      121 ttttatacat tttatggtat tatcatttta ttagttatta taaaacaatc gttagaaagg
      181 acgtcattat gcaagtatac ggaattgatg cggtgcttcg aatcgtgagt catttggcgt
      241 ttatctactt agccttctgg tctttacgtt cgttacgcat tgaaactttt ttcagaccaa
      301 tgcataatac gcaaattaga atggcaatcg tacttttttc aattttttta gggtacactg
      361 caagtaattt tttcttagaa gttatcgctt tatgccgaaa tcttttcgtt agcctgtaaa
      421 ttttaaaaaa aggtactatt ttaaaagcaa ggatgttata atagaaaaga ttttgagtaa
      481 gtgtatgctc attggaattt tttggaggga acaacatgga agagatcatc gtaagaggtg
      541 gcaatcaatt aaatggaaca gtaagaattg aaggagctaa aaatgctgtt ttgcctattt
      601 tagctgctag tttgttagca gaagaaggta ttacgacatt agacaacgtt ccgattcttt
      661 ctgatgtctt cactatgaat caagtgattc gtcacttaaa cgtagatgta gattttgatg
      721 aacaaaagaa tcaagtaaca atcgacgctt caagacaatt agaaatcgaa gcgccttatg
      781 agtatgttag tcaaatgcgt gcttcaatcg ttgtgatggg tccgctactt gccagaaatg
      841 gacatgcaaa agtagctatg cctggtggat gtgcgattgg taaacgacca atcgatttgc
      901 atttaaaagg atttcaagct ttaggagcaa aaattattca aaaaaacggc tatatcgaag
      961 caattgcgga tgaattgatc ggtaacacga tttatttaga tttccctagt gtaggtgcta
     1021 ctcaaaatat catgatggct gccgtaaaag caaaaggaac aaccatcatt gaaaacgtag
     1081 ctcgcgaacc agaaatcgtt gatttagcaa acattttgaa taaaatgggc gctcaagtct
     1141 atggagctgg tactgaaaca atgcgtattg aaggcgtgga tcatttacat gcagtcaacc
     1201 actcaatcgt acaagatcgt atcgaagcag gtacctttat ggttgctgct gcaatgacac
     1261 aaggcaatgt gctgattgca gatgctatct ctgaacataa ccgcccattg atttctaaat
     1321 tgatcgaaat gggtgcagaa atcattgaag aagaaggcgg agtccgtgtc atcggaccaa
     1381 aacatatctt acctacagat gtaaaaacga tgcctcatcc tggtttccct acagatatgc
     1441 aagctcaaat gacagctatc caattagttg cagaaggtac aagtgtagtg actgaaacag
     1501 tgtttgaaaa tcgtttccaa catttagaag agatgcgccg tatgaacgct catgtcaaaa
     1561 tcgacggaaa tgtagcaatc atggatggaa accatgaatt gcaaggtgca gaagtatatg
     1621 caacagactt gcgggcagca gcagcattag ttttagctgg cttgaaagca aatggaatca
     1681 cacgtgtgcg taacttgaat tatctagacc gtggatatta taatttccat atcaaactac
     1741 agcaattagg tgcagacgtt gaacgtgtag acatggatca aacttctgct gaaaaaacag
     1801 cacaaacgat tgcttaagga agtggctgaa tgagtaacca acgatatatc ttattaactt
     1861 tgataaagat tttaatggtg atcttattgc ttatccttct ttttgtggcc ggaacgatga
     1921 tcggatatgg tgtcatcggt ggcggcaatc catttaaagt atttcagcca agtttgtgga
     1981 ttcatattcg agactttttt cattgataaa taagaatcac agtggttcta gtcgatatga
     2041 agtgagccat gaagcagaac atgataaaag aaaacagaag ggaacgttga cgttagtcgc
     2101 gatccctttt tttagtaaaa gaaaagaagg aatctaattg atgaagaaga tattgattta
     2161 tttagttaga ggatatcagc gctttatttc gcctgcattt ccacctagct gccgttatta
     2221 cccaacctgt tccaattata tgataaaagc aattcaaacc catggcgcgc taaaagggac
     2281 ggtaatgggg actgcacgga tcttacgctg tcatcctttt gtcaaaggcg gtatcgatta
     2341 tgtaccattg acattccgat taacgcgaaa taagaaagat aaaaaagaac cagattataa
     2401 aaaggttgtg aaaaatacgg gtagcactga gcgataaaga aacaatttgg aaaatagctt
     2461 cttatatttt gcaaaattgt gagttattgc cgaagtgttg gattttgaac accgtcaaaa
     2521 aaaaggttgt gaaaaatacg gctagcactg agcgataaag aaaaagagga aatttattgt
     2581 caaagtaaca actccgcaac tacttatatg ggggacaaaa aatgatcgaa gaactaaaca
     2641 agaaagattt accatggtcc ttgttattag atgcagatcc tgataaagaa aaagttcaag
     2701 tatatgtttc cagagggagt ggctggatat ggaaagaaaa agaaaaaaca ataggtgtac
     2761 tgatctatgt ggcaagggaa aaagaatacg aaatcgtcaa tgtagcagta accccaacgt
     2821 atcaaggaaa ggggattggc ggaaaattac ttgagacagc ttttcagaag ctttcacaac
     2881 tcgcttccag tcaaactcag atcatcatac gaaccggcag tactagcagt gcggcgttac
     2941 atccctatca aaaaatgggc tatgtggaga ttggtcgagt caaagactat tttatccatc
     3001 attactctga gcccattttt gaaaatggag atcaattaag ggatcaagtg attttagcaa
     3061 ttcacttacc taaaaatagc tttaactaat aaaatttcaa ttcaagaaaa tgctttcaaa
     3121 tgctaaatag atacattaca atagctttag caaacaaaaa ggagaatatt tttatgggaa
     3181 tattagtttt tgattttggt ggatcggctg tcaaatatgg ttgttgggat ggcaaagaga
     3241 ttaaaggtaa gggacaattt gcaacgcctg aatcttggga agaaatgaaa gcgcaattat
     3301 ttcaagttta taaaaaaata ctcgtttctt ttgaagtcga aggaattgga atcagctctc
     3361 ctggagtagt gaataatgag aaacaggtga ttgagggaat cagtgcgatt ccttatattc
     3421 atggatttaa tattttccga gatttggaaa ctctgtttca acttccagta acgattgaaa
     3481 atgacgccaa ctgtgccgga atggctgaat tttatgaagg tgctgcccgc gattatcaag
     3541 atgttgcttt tgttgtggca ggaactggga ttggcggggc gttgttcacg aaaggacaaa
     3601 tcaataaagg ggcacatctt tacggtggag aatttgggtt gatgttctta aatggcgata
     3661 agacattcag taaacttggt acagcggtac agatggcttg gcgttattgt gaacgcaaag
     3721 gtctcgataa aaatgcttat accggaaaag atgtctttga attagcagaa actggagatg
     3781 caatagcaaa agaagaagtt gagagttttt atacgtatct tacaaaggga ttattcagta
     3841 ttcaattttc gtttgatcca gaagtgatcg tattaggcgg cggggtttct gcaaaagaag
     3901 ggctcattga tgagatcaaa agccgaatga agaagctgac agaacaattc gatctgcatg
     3961 atttcgaacc gcagatttta ttatgtgaat acagaaacga tgctaatctt gtaggtgcag
     4021 cagcaaacta tctcgctgta cgtcaagggg agaattaaga gctttagaga aggtgcagct
     4081 tgcataaaag aagtgaatga aatgaatagg agatttttac ctaaggaatg ttttatttgc
     4141 tgcagtatga taaaatatct gagaggtgaa aatgatgtca aaaaaaaata aagatattga
     4201 agtgcgtata gaagaagtga aaaaagatat caaaggtaag aattatgaag tcactcagtt
     4261 atttataggg aaaaaaatga ttggtgagat cttagcctat ggaccaaaag aatatgaaat
     4321 tttctttggt gaagaagatt ttggaaaaga aaaaagtctt gaaaatgcga tagaaacagt
     4381 gattcgacac tggaatctgc acgaataaaa aaatatcaaa aaaactttca ataaagggtt
     4441 gcaaaaatct ggaaaattag gtattataac taagtcaggt tgatacaaac ctttcggagg
     4501 agtagcgaag tggctaaacg cgacggactg taaatccgtt ccttagggtt cagtggttcg
     4561 aatccactct cctccatttc attggggtat agccaagcgg taaggcaagg gactttgact
     4621 ccctcatgcg ttggttcgaa tccagctacc ccagtatacc acaatcgtaa gctctcgtta
     4681 gttcgagagc tctttttttt gctttgctac cttgttcctt gaagattctg ctgatttaag
     4741 atagagttaa gttaactaca agaattggag ggatccacat gaaatggaaa gaaaaagtag
     4801 acgaagcagc tgaaaagtta tatgatttgg tcaaatcaga aaaatacaat attgaagtga
     4861 atattccgaa aaaaggcgga aaagcggtcc gggtaaaatc aaaacgccca acgaatcata
     4921 caaaaaaatg gatggctaaa aaccgctaat aataacaaaa gatccataag tcggcatttt
     4981 catcgatttt agggtctttt gttattttct aggttggaca tatctaaaaa attgtttctg
     5041 acaataggag taggaagaac gatgtgtgtc taaatcctct gttgatggat agaaaccttt
     5101 atctttttca gttgtgaatc aggtatatac cagttataat agaagaaacg aaaggacggg
     5161 tgtacgttat gtcgaatcaa ttacctgtat atatacagat tcatgaccaa ttaaaaaatg
     5221 aaatcgaaca aggtatatgg aaagtcggag accgtcttcc ttcagaaaga gaattagcag
     5281 tgaaattcaa tgtcagccgt atgacgttga gacaagcaat tcaaaacttg gcagatgaag
     5341 gaatcttaga aagaaagatc ggttcgggta catatgttgc acgagaaaaa gtacaagaaa
     5401 aaatgtctgg tgcaacaagt tttacagaaa ttatggaatc acaaaatcga gtaccgtcta
     5461 gtcggacaat ctcttatttc ttgacttcgc caagctcaag tgaaatggaa aaactaaact
     5521 tgagtaaaga tgacacgatc ttgcgaatgg aacgtattcg tttcgcagat gatgtcccta
     5581 tttgttttga agttgccagt gtgccacaaa agctgatcga tggctacagc aaagcagaga
     5641 ttactgaatc gttgtatcgc acgctagaag aaaaaggcgg gaaaaagatc ggtccagcga
     5701 atcaaacgat tactgctgtg ttggcttctg agaaaattgc tgactattta gaggtaaaac
     5761 gaggagatgc agttcttcgt ttgcgccaga tttcttattt tgaagacggt acgccgtttg
     5821 agtatgtgcg tacgcaatac gtaggaaatc gttttgagtt ttatttggaa aaataaaaaa
     5881 caggtgatcc ctgattccgt attagaaata aaagagaatc agaatcacct gtttttatat
     5941 atgatcagtt accagttgct cgtgataaga aacaggaccc tcttcaaatt ggacttgtcc
     6001 gttcagcagc tcaatgatgg attctttaaa gtggtcagca cttttttcat ctacagcaca
     6061 gatgacgcta acttgatcag tatattggat ttcttttata aaaaaatgtt ggagttcaag
     6121 ataattctgt aattttccta gttgcggata cgtgatcatc acagagatct cctgttgcaa
     6181 ggtaccttct acgattccaa tgtcagacaa tgcttgagag accgcatgag tgtatgctcg
     6241 tattaatccg cctgtgccga gtttcgttcc tccaaaatat cgagtgacta ctgcagctac
     6301 attgatcagt tgattttttt tgaggacttc caacatcggt atgcccgccg ttccacttgg
     6361 ttctccatca tcgctgcttc gctggatttc atttttttct ccaatgatga aggcgctgca
     6421 gttatggttc gctttccaat gttctttttt tatttcttga atgaaacttt ttgcttcgtc
     6481 ctctgattct attcgtctaa gagaacagat aaagcgtgat tttttgatct cgatctcact
     6541 ttgtccattt tcttttattg tacgatagcg ctcgatcatc ttgtcacctc ttttttcatt
     6601 ttctttattc agtataaata aagttctagg ttgtttcaac actgtaacgg ttgcgcaatt
     6661 gctgtagtat gcgataataa agacaaaggc aggtggatct ggggatgcag gaacgaatga
     6721 aaaagtacga tgcaattacg cattatttga agaacaatgg cggctctcaa gtaacgttga
     6781 cctttaccca atttgacgaa ctgctgtttc ccagtaacgg acttcccaaa acagcaaggg
     6841 aaagtacaga ttggtgggca aatgattata aacaccctga aaaaggtgcc tatggctgga
     6901 taaatgcagg ctatgaagta gttgtcatca atttggacaa agaatatgtc gtgttcaata
     6961 aattagtgaa atctagttgg ctatttgatt aaataaaccg cgtttagaaa acgttgacaa
     7021 atttctttct ttttatataa tagagtaaca gacgcgaaga aaacgtttgc gtagtttgtt
     7081 ttaaaaagga gagaaaagta tggctaaaaa aacaatcatg cttgtatgtt cggctggtat
     7141 gagcacaagt ttactagtaa caaaaatgca aaaagctgct gaagcaaaag gaatcgactc
     7201 tgatattttc gctgtgtccg cttctgacgc agataacaat cttgcaaata aagacgtgga
     7261 tgtgttgtta ttaggcccac aagttcgctt tatgaaagct gattttgaga aacgtctgga
     7321 accaaaaggc attcctttag atgtcatcaa tatggctgat tatggtatga tgaatggcga
     7381 aaaagtgtta gatcaagcaa tcacgctcat aggtgaataa atagaacagt ataactatcg
     7441 aacaagcgat ccaaaaaata gatcgcttgt tttttcgtat atttttttag ggcgaataat
     7501 atgaatcatt tttggggtag aaggcttctg acgagagata ttaaagcaat agactgtgag
     7561 gaggtaaatt taccaaatta tgagaagatc gcagcagtgg aacgtttgag caactggatt
     7621 ctttgtcatc gctgtggaat gagaacgtcc gtcattgatg gacagcttgc agattacggt
     7681 tatttttgta cacattgtct atatttaggg cgatgcgaca ctcaacaaga tttatatttg
     7741 tttgatcagc ctgaagcaaa acctcgagaa gtcgtctttt tttggacagg acagcttact
     7801 gaaaaacaaa cggaaatctc aaagagaata ctttgccatt cagaaaaaag acatcatctt
     7861 atttgggctg taacaggtgc agggaaaaca gagatgcttt atcctatttt agtggaaact
     7921 ttaaaagctg gaggaagagt agcgatttgt acaccgcgag tcgatgtttg taatgaattg
     7981 tttttacgct atcgttatgt ttttccagaa gaaaaaatca cgttgctcca tgggaataca
     8041 gcaacggctt atacctttac ttcttttgtt atctgtacga ttcatcagct ataccgtttt
     8101 tatcggtcct ttgatttaat agtgatcgat gagatcgatg cgtttcctta taacggggat
     8161 gcaggattag ctcatgctgt gcaaacagcg attaaaccag acggacgatt catctactta
     8221 agtgccacac ctgataaaaa gttactaaag gagatcaagc agacgtttga catccacaaa
     8281 cttgctttac gctttcatcg gcgtttgctt cctgaacccg tcttggtttt catgaataat
     8341 tggcgacaaa aatgcaagaa caagaagaag ataaaatctt tggtgcggat cattcatcaa
     8401 ttaattaaag aaaaccatat ccttttattt tgtccaagta tcacgatgat gcatcgtttg
     8461 aaagccacac ttcaagaggc tcttccgaga tatgagatca cagatgtttc ttcaagagac
     8521 aatcagcggg cggaaaaagt ccagaaaatg cgagaagcaa aatatgacat actgttaacc
     8581 acgatgatcc ttgaacgtgg agtcacattt gaaagaatat ccgttatcgt tttaggagcg
     8641 gatcatccgg tttttactaa atcttctttg gtacaaatcg caggaagagc cgatcgtaaa
     8701 gggccttata cgaacagtca agtatatttt ttctatgaag aaaaaactgc agctattcga
     8761 aaagcttgtc aggaaatcaa agaaatgaat gaagaaggga agaagatact gtgaggtgca
     8821 gttgctgcag taaagatatc gtccaaaatt taacattcag cgagattatt ggtgtttcga
     8881 aatacagatt acctcagact tgttcagttt gtcgcgcaca atttcattta ttggaaacaa
     8941 gaggctgttc tggctgtcaa aaaccaatcc atttcaatct agagagtacg aaaaagaaag
     9001 taaattctat ccccgtttac tgcagtgact gtcaagaatg gaagaagaaa tatccgacct
     9061 atgattttca gcaccgtgcc ttttttgtgt acgatgaaaa tatgaaggcc tggctcaatc
     9121 aatacaagtt tatgggagat atccgactag caggcacctt tgcatataaa tggacgcaga
     9181 taaaaaaaga atacccgaca tacatctatt gtccaatacc tttatctgaa aaaaggcgaa
     9241 cagaaagagg attcaatcaa gtaagcgaga tgttgaaagc agcaagaatt ccttttcaga
     9301 tgctactcaa aagagagaag catctgctgg ctcaagcaaa aaacacgcga aaggaaagat
     9361 tggagatgcc tcaacctttt gaacttgcag tcaccttttc taagatacaa aataaaaaaa
     9421 tattgattat tgatgatgtc tatacaacag gacagactct tttccacgca gcagattgtt
     9481 tgcagatcgc acagccgaaa atcatccgaa ctatttcttt ggcgagatga attagatgtg
     9541 gaaatttgat agcgatttaa tttgcaaata acctagagtt cgatataata gaattaagaa
     9601 gagggaaaga gtggtccttc tccttcttga ttgtggtaga cgaaaggggt aatttttatg
     9661 tttagatata atgtacgcgg cgaaaatatc gaagtaactg aagcgattcg cgactacgtt
     9721 gagaaaaaag taggcaaact agaacgctat ttcagtgacg cacccgatgc aacggcgcat
     9781 gtgaacttaa aagtttatac agaaaaaaca gcaaaagtgg aagtaacgat cccattacct
     9841 tatcttgtat tacgtgccga agaaacttct ccagatttat acgcaagtat cgatttggtt
     9901 gttgacaaat tggaacgcca aatccgcaaa ttcaaaacaa aaattaatcg taaatcccgt
     9961 gaaacgggca tgaatactgc aaatgctgct gctttattca atgaagagga cgaaaacgaa
    10021 aatgattctg aattagatat cgtacgtacg aaacgccttt cattgaaacc aatggatagt
    10081 gaagaagcag tcttgcaaat gaacatgctg ggacataact tctttatttt tgaagatgca
    10141 gaaacaaatg gtacaagtat cgtttaccgc cgtaaagatg gaaaatacgg actgattgaa
    10201 acagactgat cagccttact taaataacaa cgaaaacttg cagcagctac tgacagctgc
    10261 cgcaagtttt tttgagtttc ctcctctttt agatgaaata tgaataaatt atgaagttct
    10321 tactttaata cgcttaagat atttcaatta tctataacta atgataaaat gaaaagtaaa
    10381 gtctacatag tctggataat acacagaagg aaaaggagat tacacaacgc atggccaatt
    10441 tgattcgttc acttatcgaa aacgataaga aagaactaaa acgtttagat agcattgcaa
    10501 aaaaagttga agcatacgct gaccaaatgg cagcattaac tgatgaacaa ttacaggcaa
    10561 aaactgctga attcaagggc agataccaaa aaggagaaac attagatcaa ttacttccgg
    10621 aagcttttgc agtcgtccga gaagcagcaa aacgtgtatt aggtttatat ccataccacg
    10681 tacaattgat gggtggaatc gtgcttcacg atggaaatat ccctgaaatg cgtactggtg
    10741 aaggtaaaac cttgaccgca acgatgccag tatatctaaa tgctctgact ggcgaagggg
    10801 tacacgtagt taccgtcaat gaatatttag caacccgtga ctcgacggaa atgggcgaac
    10861 tttataattt cttaggatta acagtggggt tgaatatcaa ctcaaaatct tcggatgaaa
    10921 aacgcgaggc ttataattgt gatatcactt atagtacgaa taatgaatta ggatttgact
    10981 acttgagaga caatatggtc gtttaccgcc atcaaatggt acaacgtccg ctgaattatg
    11041 ccattgtcga tgaagttgac tcaatcttga ttgatgaagc gagaacgccg ctgatcattt
    11101 cagggcaagc agaaaaatcc acagctttat atacaagagc agacaacttc gtgaaacgtt
    11161 tgaaagaaga agaagattat aaaatcgatg tacaatcaaa aacaatcagt ttaaccgaag
    11221 cagggatcga aaaagcagaa gaaacattcg gtttggataa cttatacgac attgaaaata
    11281 ctgcattgac ccatcacttg gatcaagcat tgagagccaa ctatatcatg cttttagata
    11341 tcgattatgt tgtccaagac ggaaaagtac tgattgttga ccagttcact ggtcggatca
    11401 tggatggacg ccgttattca gatggacttc accaagcaat cgaagctaaa gaaggcgtag
    11461 agatcgaaga cgaaacgaaa accatggcga cgatcacttt ccagaactat ttccgtatgt
    11521 acaagaaact tgcgggaatg actggtacag ctaaaacaga agaagaagaa ttcagagaaa
    11581 tttataacat ccaagtatac cagattccga caaatcgtcc ggttatccgt gatgatcgtc
    11641 cagatttgct ttacccaaca ttacaaagca agttccacgc tgtcgtgcaa gatatcaaag
    11701 atcgctaccg caaaggtcaa cctgtgttag tcggaactgt tgcggttgaa acatctgagt
    11761 tattatcaga tatgctgaat caagaacgta ttcctcatga agtgctgaat gcgaaaaatc
    11821 actttaaaga agcagagatc atcatgaatg ctggtcaaaa aggtgctgtg acgattgcta
    11881 ccaacatggc tggtcgggga acagatatca agttaggact tggggtaaga gaacttggtg
    11941 gtctagcagt tatcgggaca gaacgacatg aatctcgtcg tatcgataac cagcttcgag
    12001 gacgtgcagg acgtcaagga gaccctggta tgtcacagtt ctacttgtca ttggaagatg
    12061 atttgatgaa acgattcggt tcagaacgaa tcaaatcttt cttagatcgg atgaagatcg
    12121 gtgaggaaga tgctgttatc caaagcaaga tgctgacgaa acaagtagaa tctgcacaaa
    12181 aacgtgtaga aggaaataac tatgacacac gtaaaaacgt cttacaatac gatgatgtga
    12241 tgcgtgaaca acgtgaagta atctataaac agcgacaaga agtaatcatg gaagaaaaaa
    12301 gcttatccaa gccactaatg aacatggtca aacggacaat cagtcgtgta gtagatgcgc
    12361 atacacaatt agagaaagaa aattggaatt tggaaagtat catcgatttt gccggcagta
    12421 ctcttgtcca tgaagatagt attactcttg cagatatcga aggaaaaaca gctcaggaga
    12481 tcaaagagta tctaaacgaa cgagcacaag aagtatttaa taccaaagcg gctcaactaa
    12541 atggtccaga gcaactgttg gaatttgaaa aagtcgtgat cttgcgtgtt gtggactcta
    12601 aatggacaga ccatattgat gcaatggatc aattacgtca gtcaatcggc ttacgtgctt
    12661 atggacagaa caatccatta gtcgaatatc aaacagaagg ctataagatg tttgaagata
    12721 tggtaggcgc catcgagtat gaagtcactc gaatctttat gaaagctgag atccgtcaaa
    12781 acgtacagcg tgaacaggtt tctcaaggaa cagcttctca ctcagaagac ggagatgcca
    12841 aagaaaatag taatactagc gcgaaaaaac agccaatcca tgtgaacaag gtcggaagaa
    12901 atgatccttg tccttgtgga agcgggaaaa aatataagaa ctgtcatgga aaaaatgcaa
    12961 actaatttct gacagcagga ttggtgagac aaattctttt tttaagaatt cgtctcacct
    13021 tctttctgtt atttgctgat tctgttagac tagatataat gaaaaaagta tagtgaaaga
    13081 aggaaaaaac atggaaatat ccgaaattcg aaatctcctg acagaaatgc aggaaaaggt
    13141 cacaagcttc aggaggtctc tttgacttag accagctgga agaagacatt gcggaagcag
    13201 aagcaaaaat ggctgaacct ggtttttggg atgatagcga agctgctcaa aaagtgatca
    13261 gtgaaaacaa tgcaaataaa gaaacttatg atcaattcca ttctcttgct gaagaactgg
    13321 aagaaatcga attgatggtg gagatgttgc aggaagaacc tgatgcagcc atgcaagaag
    13381 aagcagaaac acgtatcaat gcgctagatg aaaaaatgaa gacatatgaa ttatctatgt
    13441 tgttagatgg tccttatgat cgaaacaacg ccatcgttga attgcatcca ggggctggcg
    13501 gaacggagtc ccaagattgg ggcagtatgc ttttacgtat gtatacacgc tgggcagagc
    13561 agcatgggta tcaagtagag acactagatt atcaagcagg tgatgaagca ggaatcaaga
    13621 gtgtgaccct actgatcaaa ggacacaacg catttggtta tctgaaatca gaaaaaggtg
    13681 ttcatcgcct tgtacgtatc tcgccttttg actcagcgaa acgacgtcat acttcttttt
    13741 gttcggtaga tgtgatgcca gagcttgatg aaacgatcga tatagaaatc aatccagatg
    13801 atttgaaaat cgatacgtac cgagcaagtg gtgcaggtgg tcagcacatc aataaaacag
    13861 aatctgctgt acggatcact cacttgccta caggaacagt agtagctagt caggcacaac
    13921 gttcacaatt gaaaaaccgt gagcaagcga tggggatgtt aaaagctaag ctttatcaat
    13981 tagaagtgga aaagaaagaa caagaagcag ctgcacttcg aggagaacaa aaagaaatcg
    14041 gctggggttc acagatccgt tcttatgttt tccatccata ttctatggta aaagaccatc
    14101 ggacaaacgt agagacagga aatgtccaag cagttatgga cggtgacctt gatttattta
    14161 ttgacggcta tttaaaaatg catctacaat aagaacccat tgttaaattg aaataaaatt
    14221 gtaagatttt gcaagcatgt tgaaacattg ccatgctata atagctaaag actgaaaaag
    14281 atatggagag aacgccatga ttgaaatgaa ggatgtaatg aagaagtact caaatggtac
    14341 gaccgctatc cgcaatctct caattgtcat cgaacaagga gagtttgtct atgtggttgg
    14401 tccgtctgga gccggtaaat ctacattcat caaattgatg tatcgagaag aaaaagcttc
    14461 aaaaggaact ttgaacgtcg caggtcatga tctaatagca atcaaaaacc gcgaagttcc
    14521 ttatctacgc agagaaatcg gcgtggtgtt ccaagactat aaattattac caagaaaaac
    14581 agtatatgaa aatgttgcgt acgctatgca agttatcggc agaaaacctc gggatatcaa
    14641 aaaacgagta atggaagtgc tggaccttgt tggtttgaaa cacaaagttc gagtctttcc
    14701 tagcgaatta tccggtgggg aacagcaacg tgtctctatt gctcgggcaa tagtgaatac
    14761 gcctaaagta cttatcgcgg atgaaccgac aggaaatctg gatccagaaa actcatggga
    14821 aatcatgaaa ttattagatc ggatcaatgc ccaaggaact actgtcgtga tggctacaca
    14881 taacagtacg atcgtaaata cgattcggca ccgagtaatt gctatcgaaa acggccgcat
    14941 tattcgagat caagcggaag gggaatatgg atacgatgat tagaaccttt ttccgccatt
    15001 tattagaaag tatcaagagt ttgaaacgta acggctggat gacagttgct tcagtcagtg
    15061 cggtaacgat cacattgact ttagtcggtg tattcatggc agttatcatg aacgcaacta
    15121 aattagctca agatatagaa ggaaacgttg atgtctctgt attcgtagat atcggtacaa
    15181 aagacgaaga gatgaaaaag ctagaaaaag aattgaatga cttagatcat gttaaaaaag
    15241 ttgaattttc aagcaaagaa caggaattga aaaaaatcca aaacgaaatg ggtgattcct
    15301 ggaaattgtt tgaaggtgac aataacccgt tatatgatgt gtatgtagta agtgctactg
    15361 atccttctta tacaaaacaa gtagcagaag aagcagcaga tttgaagaat gtttctcgtt
    15421 ctgattacgg tggagcttcc tcagatcgga ttttccaaat cgctggtgca gttcggacat
    15481 ggggattagt tgcagcggca ttgctgctgt tcgtagcgat gtttctcatt tcgaacacga
    15541 ttcggatcac gattttgtct agacagcgtg aaattcaaat catgcgttta gtcggtgcaa
    15601 aaaatggata tattcgctgg ccgtttttcc ttgaaggtgg ctggatcggt ctgatcggtg
    15661 cgattcttcc gattttgatc atcacatttg gttatgcatg gtttttccaa ttgatcaatc
    15721 catcattatt acgttcccat tattcattga ttcatccaca aaatatcgtt tggaaaatca
    15781 atcttttaat ggtcggcatt ggtgtgatca ttggttcact tggctcgatc atttctatga
    15841 gacgtttctt gaaattctaa taccacaata taaataaaat gagaaagttg gacagtttcg
    15901 ttccagcttt ctttttttat tggctaggag aatagaagga agcggctttt ttccctttac
    15961 tttaaaaaac aacttgggta ttcttaagtt ggtagaagaa gaggtgaagc cgtgaaaatt
    16021 atcgttgaca atttttttac gatccttttg attatgaata ttttgctttc ctttatcatc
    16081 gtttttcgag aaagaaagga aactgctcaa acatgggcat gggtgctcgt tttgatgttc
    16141 attccagttg taggatttat tttatatatt tttttaggaa gagggatttc caaagagaaa
    16201 atctttgatt tgaagatcca agggaaaatc ggtaagaacc tggaaattga agaagacaaa
    16261 caggcattga tgcgagggct ttatcctcat ccgcctactg gacaagtaga tgtgaaacaa
    16321 ttgatctata tgctcacggt atttgaaagt acgctctata cgactaaaaa tgaaatcact
    16381 ttatttacag atggtcgtga aaaattcgat gcgctgattg aagatatcaa gcaggcagaa
    16441 gaccatatcc acttccagta ttatatttat cgcagtgatg cattaggtga agaagttcga
    16501 gatgcattga cagaggcggc aagacgaggg gtaaaagtca gagtccttct tgatgcatgg
    16561 ggttccacac aagtttcttc cagctttttt cagaatttga aaaaagcagg cggagaagtc
    16621 gcatttttct ttcctctatt tgttccgtat attaatcctc ggatcaatta tcgcaatcat
    16681 cgtaaaatcg tagttattga tgggaaaatc ggatatacag gtgggttcaa tgttggaaat
    16741 gaatatctag gattggtaaa gaaatttggt tattggcgag acaatcattt gcgtatttac
    16801 ggagaagcgg tgtatacctt gcaaaaccgt tttttgatgg actggaattc tcagcataca
    16861 aaagaagtag gctattctcc caaatttttt ccgtccattc actctactgg tgatatagct
    16921 gttcaaatcg tgacaagcgg accagatacc gaacatgaac aaatcaaaat gacttatttg
    16981 aaaatgattt cgttagccaa acgggagata ttgatccaaa cgccttatta cataccggat
    17041 ggatcgattc atgaagcact gaagttggct cttctttcag gcgtaaaagt tcatatccaa
    17101 atacctaaca aaccagatca tcttctcgtt tactgggcaa cgtattcttt cgctgcagag
    17161 ctaatcgagt atggagcaag gatcgaaaca tatgagaatg gatttattca tgcaaagacg
    17221 atgattatcg atggtggcat tgtttcagtt ggctcagcaa atatcgatgt tcgttcattt
    17281 cgcttggact ttgaggtcaa tacactggtt tatgatgaac gaatggcttc acgtgtaaga
    17341 aaagcttttt ttgaagactc gaaaatttct acgcatctca caaaagaaat gtatgaaaac
    17401 cgaggaataa tcatcaagat gaaagaagga ttagcaagat tgatttcgcc gctcctttaa
    17461 aacataaaaa ggacatgaga accatcaaca gatggagatc tcgtgtcctt ttctaacggg
    17521 gccatttctg agagtacgtc agaaatggag aaagcgtctc gcatgagcga aattaacttt
    17581 ggggtatctc ttgttttttg gggaaagcaa gaaataagct aatatatgct attaaaagag
    17641 acacatttca aagccagtat agcatagaaa cttttaatga gatagttttt tttacaatta
    17701 tgagaattta ttttgttatt taatgatttt gattcgtttt catcaagtaa tgaaaatgat
    17761 aaaataaaca tactttagga ttaaaggaga tttctaatga aaaaacttaa attattctta
    17821 ccaataatag gattagctat tcttttatca ggctgttcaa agtggattga tcgaggagaa
    17881 tccattacgg ctgttggctc ttctgcttta cagcctttag tagaaacagt tgctgaaact
    17941 taccagacag aaaatcctgg aaaattcatc aatgtacaag ggggcggaag cggaactggt
    18001 ttgtctcaag tacagtcagg agcagtcgac atcggaaact ctgatttgtt tgcggaagaa
    18061 aaatcgggga tcgatgcctc agcattagtc gatcatcgag tagcagttgt tgggatcact
    18121 cctattgtga acaaggatat tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta
    18181 tttacaggcg agatcaaaaa ctggagtgaa gtcggtggaa aagatcagcc agtagttata
    18241 ttgaaccgcg cttcaggaag cggaacccgt agcacgtttg aaaaatgggt attggacgga
    18301 gaaacagctg ttcaggcaca agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc
    18361 gacacacccg gtgcaatcag ctatgttgct ttttcttatg tttccgatga tgtagatacg
    18421 ttgaatgtta acggtgtaga accgacagat gaaaatgtga caacgaacaa atggacgatt
    18481 tgggcatatg agcatatgta cacaagagat catccaaaaa aactgacaaa agaattttta
    18541 gactatatct tatctgatga agtacaagat aacatcgttg gagaactagg ttatatccct
    18601 gtttctcata tgaaagtcga acgtgattgg caaggaaata tcgttaaaga gtaatcttta
    18661 cacaaaattt acaaacttag ctaaactttt acatgttatt aaaaccaaat tgatgttcaa
    18721 tttgtacact gagtgagtat gttaaacctg attaaggggg agtcatctgt ggaagatgtg
    18781 aaaaaagtat tattaacaaa atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc
    18841 agttttttat gtatcgcgct gatcgtgttg gttgtattat ctatttttta ttttgtagct
    18901 agtaaaggat tggccacctt ttttactgat aagatcaatg tatttgattt tctgtttggt
    18961 acagattgga atccaagcca aatcggagca aatgggaaac caatggtagg ggcattgcct
    19021 atgatcacag gttcgtttat tgtgaccttt ttatctgcta tcgttgccac gccatttgcc
    19081 attggtgcag cagtattcat gacggagatc tctcctaaaa acggaacgaa gatcttacag
    19141 ccggtcatcg agctgcttgt aggtattcct tctgtcgttt atggttttat cggtttatca
    19201 gttatcgtgc cttttgtcag aagtattttt ggcgggactg ggtttggtat cttagctggt
    19261 acatttgtgt tgttcgttat gatcttgccg actatcacaa cgatgactgt tgatgcatta
    19321 aaggcagtgc cacggcacta tcgggaagct tcattagcgc ttggtgccac aagatggcaa
    19381 acaatctaca aagtcgtgct acgagcagcc gtgcccggga ttttaacagc agtcgttttt
    19441 ggtatggctc gtgcgtttgg tgaagcctta gctatccaaa tggtcatcgg taatgcagca
    19501 ttgatgccaa caggtcttac tactcctgct tctacattga cgtccatctt aacaatggga
    19561 atcggaaata caatcatggg aacagttgaa aataatgtgt tatggtcatt agcgctgatc
    19621 ttgctattga tgtcattatt ctttaatatc gtgatccgta tgattgggaa gaaaggagag
    19681 ctgaaataat gaacgcaaaa cgctcagata aaattgctac aggtgtttta tatatagttt
    19741 cggggatcat cgtattgatt ttagcagctt tactacttta tatcttagtt cggggaatac
    19801 cgcacatttc atggtccttc ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa
    19861 tcgggatcca gctatttaac tcgatttatt tgcttttgat cacgatgatc atcagttttc
    19921 ctatttcttt aggtgcgggt atctatcttt cagaatatgc tggaaaaaac tggctaacag
    19981 atgtgattcg cacgtcgatt gaaatcttga gctcgttgcc ttctgtcgtt gtcggtttat
    20041 tcggtttctt ggttttcgtt attcaattcc agtatggttt ctcgattatt tctggtgcat
    20101 tggctttgac tgtattcaac ttgcctcttt tgactagaac agttgaggag tctttacaag
    20161 cggtccatta cacacaacga gaagcaggat tggccttagg tttgtcacgc tgggaaacag
    20221 tgataaaagt ggtggttcca gaagcgacac cagggatttt gactggtgtg atcctaagtt
    20281 ctggaagaat ttttggtgaa gcagctgcat tgatctatac ggctggacaa agtgcacctg
    20341 cgttagattt tacgaactgg aatccattaa gtgtttcaag cccgatcagc attttccgtc
    20401 aagctgaaac attagctgtc catatttgga agatcaatac agaaggcacg atgcctgatg
    20461 gtgtttctgt ttcggctggc gcttctgccg tgttgattat tgctgtttta ctgttcaact
    20521 tcggcgctcg tgcgattggc aaacgattat accgaaaaat gacttcagcc taaggagaag
    20581 aagcaaaatg aaaaaatata actggaatga aacaaatgtc atcaccatgc caaaagagaa
    20641 agaagtagca ctatatacgg atgatctaca tgtctggtat ggcgacaatg aagcaatcaa
    20701 aggagtggat ctggaatttg aaaaaaataa gatcactgca ctgattggtc cttccggctg
    20761 cggaaaatct acgtatcttc gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa
    20821 aatcactggg aaaatcatgt atcaaggcgt agatattaat gcgcctcaag tggatgttta
    20881 tgagatgcgc aaacggattg gcatggtttt tcaacgtcct aatccgttca gcaagtcgat
    20941 ttatgacaat attacatttg ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga
    21001 tgaaatcgtt gaaacaagct tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt
    21061 ggacaaaagt gccttggctt tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc
    21121 gatcgcgatg aaacctgata tcttgctgct agacgagccg gcaagtgctt tggaccctat
    21181 ttcgacagga acggttgaag aaacacttgt aaacttgaaa gaagactata cgatcatcat
    21241 cgtgacgcat aacatgcaac aagcagctcg tatcagcgac tatactgcct ttttctattt
    21301 aggaaaagta atggaatatg acaaaactaa caagatattt accagaccaa aaattcaggc
    21361 aaccgaagat tatgtgtccg gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga
    21421 gattatttca tctaaagatc ttcatctgta ttatggaaag aaagaagcac taaaagggat
    21481 cgatctaagc ttcaatcaag gggaaatcac tgcgatgatc ggtccttctg gttgcggaaa
    21541 atccacttat ctgcgttctt tgaaccggat gaatgacctg attccgaatg taacgatcac
    21601 cggaagtgtt ttatataaag acaaagacat ctatggtcca aaaacagatg ttgttgaact
    21661 aagaaaagaa atcggcatgg tattccagca gccaaaccca tttccttttt ctatctatga
    21721 aaatgtcatc tatggattga aattaaaagg ggaaaaagat aaaaaagtcc ttgatcaagt
    21781 agttgaagaa agcctgaagg cagcgtctgt ttgggatgat gtgaaagaca aattgcataa
    21841 aagtgcttta tctttatccg gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc
    21901 agttaatccg gaaatcatct tattggatga accaacaagt gcgcttgatc ctgtttctac
    21961 tgggaaaatc gaaagtatgc tgttagaatt aagagagcgg tatacgatga tcatcgtgac
    22021 ccataatatg tctcaagctt cacgtatttc agataagacg gcattctttt tagatggtca
    22081 cttgatcgaa tacaatgata cgaagaagat attcatgaat ccagaaaaac aagagacaga
    22141 agattatatt tcaggtcgat ttggataagg aggaaacgga atgctacgta cacaatttga
    22201 agaagaattg ctgaatctcc ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc
    22261 cgtgcacaag tcagttcgtg catacatcaa acacgataaa atcttagccc aagaagttat
    22321 cgaaaacgat ataaatatca ataacatgga aacacgcttg gagaagaaaa gttttgaaat
    22381 gatcgcattg caacagccag ttacaactga tttgcgtatg atcattacgg tcatgaaagc
    22441 tagctcggat ttagaacgta tggccgatca tgcggtgtct gttgctaaat ccacgattcg
    22501 cgtaaaaggg cagacgagaa taccggaaat cgaaaaagag atctccgata tgtccgatta
    22561 tgtgaaaaaa atggtggaca acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc
    22621 acgtacgatc gccaatatgg atcaaagagt caatgactat ttcaatcgaa tctataatgc
    22681 aacaatcaaa aatatgcagg aaaatcctga gacagtcatc agtggaacag actacttgaa
    22741 tgttgccagt tatctagaac ggatcggaga ctatgtgaca aacatctgtg aatggatcgt
    22801 ctacttggct acagggaaaa tcactgagtt aaatacgaac cacaatgaga ttttctaaac
    22861 tgaagctgtg attatgccgt ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa
    22921 aagctgttct gcatcaagta ataagaacag tcaaacaaaa aatagctcct catatttttc
    22981 gtttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt cgggaaaggg
    23041 tttgtgacaa aacatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca
    23101 aaaaaaaatt agcatcatca ctatattttt tatttgtgtg cttacttcat gggttattca
    23161 gcatcaagcc gctttatatg aagtacctgt catcgagata gaaaaagtac gaataaaaga
    23221 ggatcatcaa ttgattacag gaaaactgat gaacacggat caaaaaggag aatcgattta
    23281 tttatcggtt ccttatcaaa aaaatcaaat agaaacagca aatataaaag cgagacaaat
    23341 gtatctagtc caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa atgatggctg
    23401 gcttttcttc ttcacaagta tcttcattgg cgccatgttg cttattggtg gaaaacaggg
    23461 tctatttgct ttaggaagtc tcatcatcaa tatcgtttta cttttacttt tgttgctcat
    23521 ctttactaaa acagaaggga tttctctatt aaatatatct atcctgttcg tcgttatagg
    23581 gatgattatt agcatattgg ctttagatgg atggaataga agcagtataa tcaaaatttc
    23641 agctgcagtt acgagtgttt ttttagcatt ttttatttgt cttttgacaa tgaatcattg
    23701 gaaagaccaa ggactgcgct atgaggaact gaattatctg acacgaccgt atcgttctgt
    23761 ttttctttct agcgtactga ttggaacagc tggaggaaca ctcgataccg ttatcacagt
    23821 cattgcgaca ttagaagaat tgactaagcg acaaccagaa ataacagcga aacagttatg
    23881 gctttcagga aggcaagtgg gtcgagatgt gattggctcg atgaccaata tccttttgtt
    23941 cgtctatatc agtgggtcag ttccatcgct tcttttatat ttaggaaatc aatggtcgtt
    24001 caaagaaacg atagaacagc atttatcatt ggaattttta cgcgtcattg ctggcagctt
    24061 aggaatagta ttatcgatcc ctatctcggt cctctttttt ctgactgtaa ggaggctgaa
    24121 acgatgaatg tacttattat acttgttggg attttagcaa tcagcgtgct acttattggc
    24181 ggtacgcaag gtatgtatat tcttttaggc ttattcatca atttagggat ctttttcctg
    24241 ttgttattcg gttatcatca gaagtggccg atactggcat tgtcggtcat aggattttta
    24301 ttgattgctg tggttatctt attttttatc aacggttata atctaaaaat gcgtgcagct
    24361 tttgtgagta tactgatctt tttattttgt ttcctactat tgcttcctat cactgatttt
    24421 ttagcaatcc aaggttttac atcggtagaa ctagaagaat tatctggctt agataagact
    24481 ttagcgattg attttagatt acttacgaga tcgttgcttc tgattagtct tagtggtgct
    24541 gttttagatg cgagtgtagc aatttctagt gggacattcg aagtatatca ggcaaatccg
    24601 caattatctt tttaccaatt gcgccattct agtttcgcta ttgctaaaaa aatattggcg
    24661 tcaacagtaa tgacacttct ttttgcattc atgggaagtt ctttagcgtt gatcctctgg
    24721 tttattgatt tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa
    24781 tatacgatgg ctattcttac cacattgtcc gcactacttg ttcttccttt aacctgcgtg
    24841 actacagcat atctctttac aaaaaagaaa gaacaactca agatggagta aaagttacaa
    24901 cttaagtcct atgacatttt tactgaatca gtgcataata tggatgtaag gaaaagcttg
    24961 caaacaattt tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat tttagattta
    25021 gtgaaaaaag gaatcctttc gactgaagaa ggcttagatt tattagaaag catggcaact
    25081 gaaaaagacg aaaagcaaat caaaaaagaa gcagacaagg taactgctag tcataaagaa
    25141 aaagatcagg caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga aatttctgag
    25201 ccagttgatc caaaggaaag agaaagacaa gatcaagaaa acttagaaaa gatcttagat
    25261 gaactagcaa ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc
    25321 gctgacaata aggcagcacg caatgagaaa caggaagcat tgatgcagct gaatacaaaa
    25381 gaagaattag gcgagttgac agaagaagaa ttagcacagc gtcagacgtt agaagcagaa
    25441 atcaaagaat tagaagctag tggcgataca ctgttagaag aacaattgaa attagaagca
    25501 gaattaaaag atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt tactgataag
    25561 tttgaattgc cagatgattg gaaagatcaa gcaacagata cactgaatca agtaggcgaa
    25621 aaaatgtcag aagctggatc acagttaggc aaattcttga agaaaacatt ccaaactgtt
    25681 tcagaaacag tgaatgacaa catggaatgg aaagatgtca gcttacgtgt ccctgggatt
    25741 gcgacaacga aatttgagca tgaattttac tatgaagctc ctgcagcaag tatccttgat
    25801 atcaaggcag caaacggaaa cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt
    25861 gaagctaaga tcaaacttta cggaaaaatg ggtgctgaac catttgaagc attctctgaa
    25921 cggagccaga ttgaggttaa tgaagatcat atctccttcc aaattccaaa taaacgtgtg
    25981 cgtgcagacc ttgttttcta cttacctaaa cgagtatacg accacgcagc tattaaactt
    26041 ctaaatggca acatcatgat tgaaacattg gaagctaaag atatttatac aaaatctacg
    26101 aatggtaaca tcatcgtgga ccaattaact gcaacgatgc tggaagtaga aggtgtaaac
    26161 ggcaatatcg atattcgaaa cgggaacatc ttagattcta ttatcgaaac agtaaatggt
    26221 acagtgacat ttggtgcgac agcagaaaac ctttctgtgt ctttggtaaa tggtgatgta
    26281 cgtcttacga ttaaagaaga taatttgaaa aaagtagaag caagttcagt gaacggaaat
    26341 gtcaaagtcg ctttaccaga tggcatcggt ttggaaggac atgctaaaac aagtcttgga
    26401 agtatcaaca gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga acggacaaac
    26461 caaatgttgc aattccgtcg tgtttcagat ggtgaaatcg cgcaagtgca actttcaacg
    26521 acaacaggaa gtatctattt aaaagataca gataaataaa gaggttgtga aagagatgtt
    26581 cagccttgag caataagaag cgattttgta aaatagcttc ccctatttta caaaattgtg
    26641 ccttattgcc gatagtctat ctcttgaaca ccgtttacca agagattatg aacctgccaa
    26701 ttatcgccga gtcacaagga ataatttcag aaaaagctcg tcacgtttta tgaatatggt
    26761 aatttgtgac cgagaatttt gcagaaagac accgattaag aagaaggttg tcacaacgat
    26821 tttcttcatt taaaatagta gaacagaggt gagacatgat gaaaaaaaag ttgacaaaat
    26881 ctaataaaaa tgttgttttg acagggactt tagcaggaat aggagaatac atcgggattg
    26941 atccgactgt tatccgtgtg ctttatgtat tcctgagctt agttgctttt ggaagtccaa
    27001 ttattttata cattttatta gctttgatta tcccatttgc gcgttctgca cgaacaaact
    27061 atggacataa caatgcatat tatgaaaaaa acaattatcg tgaacaaact cgtgaacaaa
    27121 caaaaaaacg caaagaagcg gaaaaaatcg atgatgacga atggagtgac ttttaatgtc
    27181 gtactttcaa cgtattttag ttaatacgtt aacattcata tcattggctg ttctttttcc
    27241 tgcgatggtc catgtgagca gtatttggac ggccgttctt gccagcttcg tattgtctgt
    27301 gttgaatatg tttgtcaaac cgattttgac gatactgtcc ctgcccttta cattagtcac
    27361 attgggattg tttacgtttg ttatcaatgc agcgattctt aaaatgacct ccttttttgt
    27421 gggagcagaa cattttagtt tttcttcatt cggtgcagcg ttgttagtag ctatcttgat
    27481 gtcgcttgtc aatgtaattg tcagcgaaca taacagtaga aaataaaaga aagctgtgcc
    27541 gaactatcgg cgcagttttt ttatttagaa tcaggaatgc gttttctatt acattggtaa
    27601 ttaaataaaa acgttttttt taattggtag tacttttggc tagacaatga taaaatgata
    27661 gagaacactc taatagaaat gaggtagcat gaatgtcaga agttgtaaaa gttagccaat
    27721 tagtgaaaaa attatctttg gaaatcgtga caggcgacga gaaaagctta gaccgtgtca
    27781 tcgtaactgg ggacatctca cgcccaggac tagagttgac tggttatttt aattattatt
    27841 cccataatcg tttacagcta tttggcagta aagaaatcag ttttgcggaa cggatgatcc
    27901 cagaagagcg tttgatcatc atgagaagat tatgtagtga agatatgccc gcatttatta
    27961 tttcaagagg attaccagct ccagaagaaa tgatccaggc agcaaataaa aatgggatag
    28021 ctgtactgcg ttctcccatc tcaacttctc gtttattagg agaattatcc agttatttag
    28081 atggtcgcct tgcccctcga acaagtgtcc acggtgtttt agtggatgtt tacggattag
    28141 gtgtactgat tcaaggtgac agtgggatag gaaaaagtga aacagcgttg gagttgatca
    28201 aacgcggcca tcgattgatc gcagacgatc gagtagatgt ttatcagcaa gatgaattaa
    28261 cggtgatcgg agaaccgcct aaaatcctgg aacatctgat tgaaatccga ggaatcggga
    28321 tcattgatgt aatgaatctt tttggtgcga gtgcggtacg tggctctatg caagtccagt
    28381 tggctgttta tctggaagca tgggcaaaag ataagaaata cgaccgtctg ggctctgaag
    28441 acacgaccgt tgaaatcgcg gaagtgggca ttccacaaat aaaaatccct gtcaaaacag
    28501 gacgaaacgt tgcaatcatc attgaagtag ccgcgatgaa tttccgtgca cgtacaatgg
    28561 gctttgacgc aacgaaaaca tttgaagaaa gattaagtcg cttgattgaa gaaaactcag
    28621 gcaacgacta gcaaggagtg acaatatgtt agcacaaatc aatcgagttg cttttgagct
    28681 tgtagggatt cctatctact ggtatgcatt gatcatcgta tcaggtatca ttatcgctat
    28741 gtggcttagc agcagagagg ctgttcgggt aggattaaaa gcagatgatg tgacagattt
    28801 tatgttgtgg ggattgccta tctccattat tggcgctcgc ttatattatg ttttatttga
    28861 tcttccccag tatatagctg atccaatcca aatattcaat atccgttcag gaggcttagc
    28921 tatttatggc ggactgattg caggcgcgat cacgctgtat ttttttacta aatatcattt
    28981 tatttctttg tggacttttt tagatatcgc tgcaccaagt gtattattag cacaggcaat
    29041 cggtcgttgg ggaaatttca tgaaccacga agcatatgga ccagacacga cgcgaagctt
    29101 cttagaaagt ctgcatttac cgcaattcat cattgataat atgtatatcg aagggatcta
    29161 tcgtcaacca acatttcttt atgaatcact ttggagtttg attggattta ttttgctttt
    29221 gtttttacga aagaaaccac accttttgaa aaaaggcgaa gtccttttat gttatctaat
    29281 gtggtatagt ttcgggcgtt tctttattga aggattacgt acagacagtc tttacttttt
    29341 aggtgtgatc cgtatttctc aattattatc tgctgtgtta tttgtcggta cattgattct
    29401 atttatttgg cgcagaaaaa aaggaaattt accggattat gaccgctcat taggaaaaaa
    29461 tcaacaaatt atctgatttt tagagcgtta aaaatgaaaa aaatttcagg agtggtaaaa
    29521 tgaattatag agtagcggtt ttaggaccag gctcttgggg tactgctttg gcacaagtcc
    29581 tcgccgaaaa cggacatgac gttcggattt ggggacatcg tgcttcacaa gtagaagaaa
    29641 tcaacaccca acacacgaat caacgttatt taccagatca ttatctaccc gattccatcc
    29701 aagcattcac caatatggaa gatgcagtaa aagacgcaga tgcggtgcta ttcgtcattc
    29761 caacgaaagc aatacgttct gttgcaaaag aactagtgcc taaattgaat acaaagccag
    29821 tgatcatcca tgcaagtaaa ggattagaac agggaacaca taaaaggatc tctgacatct
    29881 tgttagagga aatcccagca gaaaaaagaa aagctgttgt tgttctttct ggaccaagcc
    29941 atgcagaaga agtagcaaaa catgacatca ctacgatcac agctgcaagt acagatgcaa
    30001 aagcagcaaa atatgtacaa gagctgttca tgaataatta ttttcggatc tataccaatc
    30061 ctgatgtgat cggtgtagag atgggggcag ctttgaaaaa tattattgct attggtgctg
    30121 gcgctattca cggcttaggg tttggcgata atgcaaaggc agcaatcatg acgcgcggat
    30181 tagctgagat cagccgttta ggtgtagcaa tgggtgccaa cccgctgacc tttatcggat
    30241 taagcggaat aggcgatcta gtcgttactt gcacaagtgt tcattctagg aactggcgcg
    30301 caggcaactt gttaggacaa gggcagcccc tagaagaggt gctagccaat atgggaatgg
    30361 tcgttgaagg tgtgaatact acccaagcag cgatggaact agcccaaatg atggaaatcg
    30421 aaatgcccat cactcaaaca atctatgaag tgctgtatga acaaaaagaa atcaaaacag
    30481 ccgcaaaaga aatcatgtta cgtgatggaa aaaaagaaaa tgaattctca ttaacaattt
    30541 tttgaaggag cgaatgtata tgaaagttaa aaaagcagtt attccagcag caggattagg
    30601 aacaagattt ttaccagcaa caaaagctat ggcaaaggaa atgttgccaa ttgttgataa
    30661 accaacaatc cagttcatcg ttgaagaagc gttgaaatca ggtatcgaag acatcttgat
    30721 cgttacagga aaagctaaac gaccaatcga agatcacttt gattcaaata ttgaattaga
    30781 aacaaaccta agtgaaaaag gaaagaccga attattgaaa ttagtagaag aaacgacaga
    30841 tgtcaacttg catttcatcc gtcaatctca tccaaagggt ttaggacatg ccgttcttca
    30901 agcaagagcc tttattggaa atgaaccatt tgtcgttatg ctaggcgatg atataatgga
    30961 agacgaagta ccattgacac agcaattaat cgatgattac gaacgtactc atgcttctac
    31021 gatcgccgtt atgcaagttc ctcatgatga aacttcaaaa tacggaatta tcaatccagg
    31081 agaagtattg gaagatgggt tatacaatgt caaaaacttt gttgaaaaac cagatccaag
    31141 caaagcacca agtgatttag ctattattgg acgttactta ttgactccag aaatttttga
    31201 tgtattgtcc gagcaacaac caggagccgg aaatgaaatc caactaactg acgcaatcga
    31261 tacattgaat aaaacacaac gcgtatttgc ccgtaaattc acaggtaaac gttacgatgt
    31321 cggtgacaaa tttggtttta tgaaaacaag tattgaatat ggtctaacac atccagaagt
    31381 aggtgcacct ttacgtgagt acatcctgtc tttaggtaaa acattagaag cagaaggaag
    31441 caagatcgat cataatccaa aaggaataac agaaatcaaa gaagaaaaga aaaaataacc
    31501 cttttttcta acgagcttca gctgctaatt gaataaaaag tagttgaagc tcgtttttat
    31561 ttgtttaaaa aacacgaaaa tgctatcctt atagaaaaga gagacaagaa gggagtttga
    31621 tcatgtcagg aggagaaatt gcaggattga tcgcagcaat cgcctttgcg atacttgtcg
    31681 tttttatcgt tcgtgtgttg ttgcaagttt ctaaaacttt agaaaaagta gatcagactg
    31741 tagcagaagc aaatacgacg atcgaaatcg tcacaaaaga cgtagatctt ttgtctagac
    31801 aagtagaagg cttactggtc aaaagtaatg aactgctctt ggatatcaat ggaaaagtcg
    31861 caacgatcga tccattgttc acagcagttg ctgatttaag tgaaagtgta tcagaactta
    31921 atcattctag taaaaacatc gcgacgaaag tcggcggaat cggtaaaacg acagctaaag
    31981 cgacggttgc cggaaaagtc ggtgggacag cgatgaaatt ttttaaaaac aaaaaacata
    32041 ctgaaacgac aggaggaaaa taaaaatggc taaaaaaggt ggatttttat taggtgcagt
    32101 agtcggaggc acagccgcag caatcgccgc gttattgtta gcaccaaaat ctggtaaaga
    32161 acttcgggat gaattagctg cccaagcgga tgatttgatg gatatggctt cagattacac
    32221 agatatggcg aaagaaaaat cgactgagtt aacaggtatt gcaaaagaaa aagcgcaaga
    32281 tctttctcaa caagcaggtg atctagcagg tagtgtcaaa caaaaagtaa gtgaatcagc
    32341 agatgaagca ggaagtgaat ctgacgatat cttgttaaat cttaaagaac aatcagctga
    32401 cttatctaat cgtttcaaaa aaacggcaga agatatgaaa aaacctgcag atgaattagg
    32461 aagtattgct aaagatactt cagaagatat catgattgat gtaaaagacg ctgctggtaa
    32521 agcaggagat actgtcaatg aaggaatgtc agaagcaaaa ccagttgcag atgactcaaa
    32581 agaaatgatg aaaatggcaa aagaagatgc gaaagaagct aaaaaagaaa taaaagatac
    32641 aatgacagat tcatctacaa atcactaaaa tcacaaattt atagaataaa aaggtgggag
    32701 cggactagct ctcacctttt ttactcttat ttttctaaaa caagtaattc ttttggtgta
    32761 gtcgtgaatg gcacacagcc atcgcttgtc acatatacac agtcttcgat acgtacccct
    32821 actttttcag gaatatagat tcctggttca agagagaagc acatcccttc ttcgattact
    32881 aagtcatttc cttctacaag ggaagggaat tcgtggacgg ttgttccgat cccgtgacct
    32941 aaacggtgat tgaaatattc gccgtagcca tagctgttga tcacgtttcg tgcgatttga
    33001 tcaagttcac ctgctgtcac acctggacgg acggcttctt gtgcggcaag ctgtgcttca
    33061 agagtgatgt tgtagatttt ttcttggaag tcggttggtt tttgataagc aattgtacga
    33121 gtagcatcac tgcaatagcc attccaaaca acgccaagat cgaataacac aagttcattc
    33181 ggtgaaatct tcgtatttcc tggtgtacca tgagggcttg cagcatttgc tcctgcaagg
    33241 actaatgtgt cgaaagacat ttgtgaaaca ccttgttttt tcaattggta ttcaatttct
    33301 gcaatgatcg cttgttcagt cacgccttct ttcactgctt taaagccaat ctcaaatgca
    33361 acatcggccc atttaccagc ttcgatcagt ttgtcttttt cctctagagt tttgatcagt
    33421 tgtagacgtt ggatgatgga agttagatca gtgtaagaat ccgttcccgt caagatatca
    33481 gagatagcat cgaagcgctc gacagtcaag gcgttctttt caatccctac attgccaggc
    33541 tgaccatagc gagcacgaag ttcttttccg atgatctccc aagggttttc gctgtctaaa
    33601 tagccacgga catcatacgc ccaactactg ttttgagcat cttcaacttc caaagcagga
    33661 gtgaaaagga aaggatcttg ttcaagaggg ataaaaagag ctagtacacg ctcatgagga
    33721 tcactttcgt atccggaaaa gtaagcgatg tggccagggt cgctgatata agccaattcg
    33781 acttggtgtt gtgccatcca ttgacgtagt tcattgattt ttacgttatt catttggcaa
    33841 cctactttct aaattaattt tgtattcaat aattgtacca ctgatttatt aaaagaccaa
    33901 cgttctaaaa actgaaaaaa gtaaaaattt tcatgaaaac agagataaaa cggttgcaat
    33961 tcttgagatt atttgatatt ctattttaga aatgtaagac gtattttcag aaaacatata
    34021 aaatatgaat aggagaacac aaatggaaaa gcaaacaatc actatctatg atgtagcgag
    34081 agaggcaaat gtttcgatgg ctactgtttc acgtgtcgta aatggcaatc caaacgttaa
    34141 accagcaaca agaaaaaaag tcttggaagt tatcgatcgc ttggattatc gtccaaacgc
    34201 agttgcgcgt ggcttggcaa gtaagaaaac aacaacagtt ggtgtaatca tccctgatgt
    34261 cagcaatatg ttctttgctt ctttagcacg tggtatcgat gatgtggcaa caatgtacaa
    34321 atacaatatt atcttagcaa attcagatgg caacgaccaa aaagaagtca atgtattgaa
    34381 taacttactg gctaagcaag tggacggcgt gatcttcatg ggtcaccata tcacagacga
    34441 aatccgtgga gaattttcac gttcaaaaac tcctgttgta ttagctggct ctattgatcc
    34501 agatgaacaa gtaggtagcg taaacattga ttatgtatcc gcaacaaaag atgcagtaaa
    34561 caaattggca aaaagcggca ataaaaaaat cgcttttgtc agtggtgcgt tgatcgatcc
    34621 catcaatgga caaaatcgtc tgaaaggcta taaagaagca ttaagcgaaa ataacctacc
    34681 atatagcgaa ggattgatct ttgaagcaca atacaacttc aaagatggtc tatctctagt
    34741 cgatcggatc cataatagcg gtgcaacagc tgtctatgtt tcagatgatg aattagccat
    34801 cggtattttg gatggtttat tagaccgtgg cgtgaaagta ccggaagaat tcgaaatcat
    34861 cacaagtaac aattcactac tgacagaagt agctcgtcct cgtttaacaa gtatcacaca
    34921 accattatac gatttagggg cggtatctat gcgcctattg acgaaaatga tgaataaaga
    34981 agaagtcgaa gaaaaaacga ttatcttgcc ttatggaatc gaagaaaaag gatctacaaa
    35041 ataataaaag gacaaccatt cagaggttgt gtgcctgccg tttaactctg cgttacaagg
    35101 agcaatttca aaaaacagca tctcatgttt gatgaatatt gtgactcgtg attcggagtt
    35161 ggcaggagaa caccgtttat ttgggaaaag gggttatgac aaaattttgt tataatcctt
    35221 tttttgtgtc ttaaaaaaga gagaaagtgg ttgaaattac atttatcgta tataaaacaa
    35281 tccgtgtata gatgctgcat tttgttcacc accatcaaaa aaataaggga ctgtgacaat
    35341 actattgtca caatccctta ttactgtgta ttctattaaa tcaatcatct ttctcatctg
    35401 attctttagt tgcttgattc gctcttgcat aagctgaagc ggtgttccag taatctgtat
    35461 agttttcatc ggtaccgcca acgccaaatt taaaggcgct tttcttcggt ccatttttcg
    35521 cccatagcga ttcaactgtt tttcctggtg tttggatgga ttttttattg acagtaactt
    35581 ttccaggtgt ttgaccagta aatccagata cttttttctt catcacatca gatgataatt
    35641 cgaatttctc atcagtgcca aaaatgtcag gttttgcttg gtaagcttga ttgatcagat
    35701 tggcaagata attagccgaa cgtatccttg ctttgctgtc cattccagta ttgtcatcgt
    35761 gccctgccca gctactgatg gtaatagacg gagtagagac aattagccat gaatctcggt
    35821 attcattggt agaaccagtt tttccgaccc aatctgcttt tcctagattt ccgtttaagc
    35881 ttgagatggc atctttgaat ggtgtagtga tttttgcatt gatcacacta cgcatcatgt
    35941 cattcatgat cgatgccgtt gcttccgaat agatccggac agagttgctt tcgtgtttat
    36001 aaattacatt tcctgcgtta tctgtgatgg aatcgatgat atagccttct tcatagacgc
    36061 cgccgtttgc taaagcctga aatccgttgg tctgttgaag cgtcgtgaca tctactgtac
    36121 ctaaaggagc agattcaacg ccccaattgt catttgccgg gtagttcatc tttgccaagt
    36181 agtgctcgta agcatattgt ttcgatccac cggaattcaa cacatcttga tataagtgat
    36241 aagccgggat attgttggac cattctagtg actcacggac ggtttggaag gtgttcgatc
    36301 cttcgttagt agcattgacg attttctcgc cagcattttc tccttcttgc caagttgttg
    36361 cataatctga tacacgggat tctgatccga taagccccat gtcgattgca ggtccataaa
    36421 caagaactgg tttgattgat gagccagctt gacgttctgt atcgaaggca tggttatttt
    36481 gattctgact gtaattccgt ccgccaacga atccgtagat gcggccagtt ctgttatcca
    36541 tcaatacatt tccggtttct acttgactgc cagcaccatc atctaacatg tatccataat
    36601 tttcaacgcc attttgcaat gcagcataga catctttatc gattgtcgaa tggattgtat
    36661 atcctttatt ttggatcgtt tgctgtgcct tttgataata agcgtcactg accgatgaat
    36721 cagacatatc agcgttgtct ttttcagcca attgttgtgc aatgattctt gttgcttcat
    36781 tcatcacagt ataatacaag aattctctgt cagtctgttc tgcgatttgt tgtggtagaa
    36841 agtcttttgt aaggtcatag gcttttgctt cttcgtattg ttctttcgta atttggcctt
    36901 cacgatacat actgaataaa acaaaatctt ttctggcaag gccggcagaa agatcttctt
    36961 ttagcgcgcc tgtgttcgtg tatggactat acgtgatcgg gctttggggt aatccagcga
    37021 tataagcagc ttgcggcagc gtcacatctt ttgcatctac accaaagatt ccttttgcag
    37081 cttcctggac tccagcaatg ttttgtccct tattattacg accaaatggt gagacattca
    37141 aatacgtagc aatgatctca tcttttgaaa agtatttttc tacttgtgca gaaagaatga
    37201 tctcgtttgc tttacgtttg aatgttgttt catctgtaag gatctgctgt ttgactaatt
    37261 gctgtgtcaa agttgatcca ccagaagagc caataccagt agcttcagat actaatgctc
    37321 gtaaaacagc tttaggaacg taaccttggt gtttatcgaa gtattcatcc tctgtgctga
    37381 taattgcagt tttcaataaa ggtgaaatct tatcagatga aatagtcgtg cgcatcagat
    37441 ctgtctggat ttttgatatc tctgtattat ctgcataaac aatctttgac gtttctgtaa
    37501 tgtttcctag ttctgtctgc agtgcttttt tagtcggcaa ttgtgtgtct tcgacgagat
    37561 aggcaaaata gccagcgcct attccaatac ctagagctgc gaacatcaaa agaagaacag
    37621 taataaaaac agtcaatgat tgaaaaactc taaaaataat cttaggaaca aaccattttt
    37681 ctttttttct tctattctgt ttttttcgag tatttttgcg tggcaaaagg gtcacctcaa
    37741 ctaatggttt tatttcgcta attatagcaa ataatagaac aaaaaaacag acaaggctgt
    37801 ttttggcagg aatttctttt ttaaaagagt taaaacgtga gaaaatgatc tttattataa
    37861 aagagttctt aagcttttct ttagagaaaa tttcagttac tatggagaac agggcaaaga
    37921 aaaatcaatc attttctttg tatatcatct taccaatata taggaattgt tagtaacaat
    37981 tgcctttcaa ggaggaatcc atccatgtta ttaggctcac acgtcagcat gagcggaaag
    38041 aaaatgctgt taggagcagc agcagaagca gccagttatg gatcttcgac ttttatgatc
    38101 tatacaggcg cccctcaaaa cactagaaga aaagccatcg aagagatgaa tattgatgcc
    38161 ggaaaagcgt acatggcaga aaacggtcta acaaatatcg tcgttcatgc cccttatatc
    38221 attaatttgg ggaatacgat caaaccagaa atgtttcctt ttgccgtcca atttctgaga
    38281 gaagagattg ttcgtgcgga agctttaggc gctactcaaa ttacacttca cccaggtgct
    38341 catgtagggg caggaacaga agccggaatt gctagaatta ttgaaggctt gaatgaagtt
    38401 ttgacaaaag aacagacggc acaaatcgct ttggagacaa tggcaggtaa aggtacggaa
    38461 atcggtcgaa catttgagga attagcgcaa atcatcgatg gagtaaaatt aaatgaaaaa
    38521 ttatccgtta cttttgacac ctgtcatacg aatgatgccg gttataatat taaagaagac
    38581 ttttcaggtg taatggaaga gtttgataag atcatcgggt tagatcggct taaagtactc
    38641 catatcaatg attctaaaaa tccgcaaggt tcgcataaag accggcacgc caatattggt
    38701 tttggaacaa tcggattcga cacgctgaat aagatcgtcc atgatccaat gtttgacaat
    38761 atttcgaaaa tcttggaaac tccctatgtc ggcaaagata aaaagaaagc ttacgcacca
    38821 tatggaaaag aaatcgccat gttcaaagca caaatattcg atccagaagt gtttcctgag
    38881 ttattagcag aagaagtgat actctaagat tttctatctg acagcagcaa aaaaggacca
    38941 ggcacattgc cttgtccttt tttgtttaag aaacaaagga gtactttttg cagtcttttt
    39001 catcatgcat cttctccccc ctctttttaa atttagacaa acaaggtata ctgttagaaa
    39061 agtaaaaaaa cgaaaagttt cacaagcaag tggcaaaacg tgattggaag gacgaaaaga
    39121 tgctgaacac tttaaaaccc ttgatcaacc aactaaaaaa gaaacataca tactttattc
    39181 ttggcatagc cctattattt acaattattc gtttgtattt atatagtaaa gcaatctatg
    39241 gaacagacat gtcggcaacc tatgatgacc agctgctgat tcgctatgct caaaatattt
    39301 tggacggtaa atggctaggg gagtatacga ctacaacact ttccaaagga atctcctaca
    39361 gcttattttt agtacttgca cataaactgc atctttctta tgctgttttg ttcggcatgt
    39421 tcagtatttt tgccagtata ttggttgcac tcgcgtttaa accgatcgtg aagaacaagt
    39481 atcttctttt aagtatctat ttttattttt tgttttctcc gattagtttt acacatgaat
    39541 attccactcg aacatatcgt aatgccctcg tgattccggc tgttgagatc attatcgctt
    39601 gtttactagc tatttattac cgcaaatacg gttctagcag aaaattgctg ccatggtttt
    39661 tagggctttc tgtcgctttt cctttctttt ggttcattcg cgaagattct atctggcttc
    39721 ttccttttac tgtagtagct ttgggtatca gtttcgtcca gatcttgcta gggcaagact
    39781 tttctttcaa agaagtaaag aagttaaaaa aacgactctt tcttctctcg aaacgggaat
    39841 ggctgaaact ttcattgttt ttacttccta tagttggctt ttccatcgtc tatcagtgga
    39901 tcgaatataa aaatgagaca acatacggta ttgcaaccat caatgaccgt tccagtggag
    39961 aatttggacg tttgatgaaa caactgatcc gaatagatga tgaaacgaac ttgaataaag
    40021 aaaacagtga gatatgggtc tcacatgctg cactaaataa agcattagcc gcatctccta
    40081 cattccatac tgtttctacc cgtattgacg aattatatac gactcacgcg tggactcaag
    40141 gtggaaaagt aaaagaatta cccggagata tcatcttttg ggcattgcga gatgtggtaa
    40201 gcgaatctgg ttattaccaa aacaatgcaa aagaaaccaa tgatttttgg aaaaaagtca
    40261 accaagaatt agaagaagcc tatcacaagg gaaccatcaa aaagaaaaaa gagttctatc
    40321 tgatgggatc aggagatgga aagacgatga gtgatctgcc aatcgtattc gatttccttc
    40381 aatcaggata cgcttatgca tggcgatata aagatttcca tcaaggagga aattatagtt
    40441 tcggaacgca agaacaagtc aaacaggctc aagaattatt aaaccttccg ctaatggaga
    40501 attggatcga tagtaatgaa ccgagaacac ctaatttcca attaacaaaa tcagcaaaag
    40561 tcgcgaatgt catcataaaa atttaccaag ttctggctat tcccgtgctg attgcttcaa
    40621 tagcagggat attacttatg atactaggaa ccgtatttgc aaaagctgat cgtgcctatt
    40681 atagttcctc tttgattgtg gtcaccggat taatactgac acaattggtt tttcttttcg
    40741 gtgtctcgtg gttttgttct tatgcgccag aaaaaaagga ttatttttta agtgtctaca
    40801 ctggcgcagg cgtgccatta acgcaattca tcgtagtaac aagcatgcta gttttcagta
    40861 aattaaatct gaaaaaggct ttatcttcta aataaaaaga cccttggtag ctagattttt
    40921 aacctaacta tcaagggtct ttgtgtggaa aaatcagttt ctattcgatt tcttctaccg
    40981 tttcctgcaa acgctgttca tattttttgt ttcctttata taaatcacca atcgtccatg
    41041 ccaacaggag aaggacaaga acaatgatta cataagcgat gtgatcagcc aatgctagtt
    41101 gagcagaagt ggcttgttca ccaaagaaac tttcgatctg tcctggtaat cctcgtaagt
    41161 tcaagtaagt tagactaatc acagagaacc agccaagaag ttttacgatg aatgaatttt
    41221 taaatcggct acccatctcc gcttggctgt tggtcatcat cagtaaaggg atcatcgaaa
    41281 acggcaacgc aaaagccaag aacacctggg attcattcat caagttattc aaagcggtat
    41341 gttcttcgat cgttcctttt gtacttgtaa acaaaacaca aatcaataca ggaatcacag
    41401 aaagcaatcg tgtgaccagt cttcttagcc aaatcgggat acgcatatgg atgaagcctt
    41461 ccatgatgac ttgaccggtc aacgtaccag taatagtaga gttctggcca gaagcaagaa
    41521 gtgctacagc aaataaagtg gataagatac cagattttgc tacagcaatc aagataccat
    41581 tgctcatagc tgatggatca gataaggctt catacagacc aaaaaaggaa ggatcttgga
    41641 cagaaccggt tttgaagaca gcgacaccca tgatcaatag cagtgcattg actagaaaag
    41701 ccatcgacaa ttggatatta gagtcccaag tagaaaaacg tacagcttgt gctacatctt
    41761 cttcgtcttg gtgatcaatc ttccgagttt gagaaactgc tgaatgaaga tataagttat
    41821 gcggcatgac tgttgcacca ataatcccta aagctccagt cagcggagtt tcgccgccga
    41881 tgaccggtga gtctgagaat gtatgactgt tagggataaa gccagcaaat aaactgtgcc
    41941 aatctgggtt agacaagcca acttgataga caaaaacgaa taaaatgacg aaaattaagc
    42001 agacaaccag tgcttcgatc ttacgaaacc cgacttttgt cagtagtaag agaacgaaaa
    42061 catctaatac agtaataaag acggcaatca ccaatggaat atcaaaaagt aagtagaggg
    42121 cgattgctgc accaatgact tctgcgatgt ctgttgccat aatggctaat tcagtcaaga
    42181 tccataaaat gacacccaat gtcttacttg tgcgtgctcg gattgcttgg gccaaatcca
    42241 tttgggtcac gattcctaat tttgctgcca tatactgtaa aagcatagca atcagactag
    42301 acatcaaaat aactgacatc aataaatatt ggaaattttg tccaccagtg atcgaagtgg
    42361 accagttacc tggatccata taaccaactg ctactaatgc acctggtcca gagtaagcaa
    42421 gaagtgtttt aaaaaaactt ttcccctttg gtacatcaac tgttccattt acttcagcta
    42481 aagataaacc atttgtatgt tcaataagtc gatgcttatg aaattgtttg ttggattgat
    42541 tattcaagaa aaaaccacct ttcctattta tctacgatta cattctacac ctcaaaaaga
    42601 aaaaattaaa gaaaaatatt cggtatgcct aatttatttt cgtggcgcgt cttgttagat
    42661 gacgaaagaa attttgttct cctctaaaaa aaccactatt ttttgcatga aactaaagaa
    42721 tacgcctttc ttttttcaat aaactttagt aaaataagtc attgagagca attcggaaat
    42781 agaaaggaga gatttatatg ttatcaacag gtattgtcgt acttattgct attattgctt
    42841 tgctaatcgg cgcagtaggt ggctttttcc tagcgcgtaa atatatgaaa gactatttag
    42901 aaaaaaatcc tcccgttaat gaggacatgt tgcgatcaat gatgatgtca atgggacaaa
    42961 aaccatctga gaagaaaatt cgccagatga tgcagcaaat gaagaaccaa gggaaaaaat
    43021 aggttctgac ccaacgactt tttacgggct gattttttca gtccgttttt tattttgtaa
    43081 tttgttagac tattcagatt attcagcaaa aagacaagca cagacaatcc taggctcttg
    43141 ataaagacaa aggaggagta aaatgtcgat ttttagaaag ttaggctggt tttttcgtca
    43201 ggaaaagaaa cattatttaa ttggggtttt ctcgctattc gcagtggcct tggtacagtt
    43261 agttcctcca aaagtgattg ggatcatcat tgatgagatt gccgataaaa atttatcagt
    43321 aaagatcatt ttcgggtgga tcttcctatt gattatcgct gctttcttac aatatctttt
    43381 tcgttatatc tggcggacga atatttgggg aagtgcagct cggttggaga aagaacttcg
    43441 gcagcagtta ttcaaacatt ttacaaaaat ggacaatctc ttttaccaaa aatatcggac
    43501 tggggatttg atggctcatg caacgaatga tttgaatgcg atccagaatg tagcaggagc
    43561 tggtatcttg acctttgcag attcagtgat tacgggcgga acaacgatca ttgtgatgat
    43621 tttgtttatt gattggcgct taacactgat tgcgctgatt cctctgcctt tgctggccgt
    43681 cacttcacga gtgctggggt cgaaactcca tgatgctttt cgtgattcgc aagaagcttt
    43741 ttctagtatc aatgataaga cacaagaaag tatcactggg atcaaagtca ttaaaacatt
    43801 tggacaagaa aaagaagatc tagcggattt tgaagaaaag attgaagatg caattattaa
    43861 aaacaaacga gtcaattttc tagactcctt atttgatcca ttcattacct tgattatcgg
    43921 tctgtcttat gttgcaacaa ttattttagg tggaaagtat gtcttggaag gcacgatctc
    43981 gatcgggcag atggtctcat ttattagtta tatcggtatg cttgtttggc cgatgtttgc
    44041 gattggtcga ttgttcaacg tattggaaag aggaaatgct tcttatgatc gcgtcaatga
    44101 acttttgcat gaaaaaacgc atatcgtgga acaactgggt gcagtgcaga ccccggtaaa
    44161 aggcgagctg aatgtagcta tttcctcttt taattatccg aaagaagaat caccagcatt
    44221 agaacaaatc aagtttgcaa tccaagaagg agaaacatta ggtattgttg ggaaaactgg
    44281 tgcgggaaaa acaacgatca tcaaattact gatgcgtgaa tacgatgatt atcaagggaa
    44341 aatccaattt ggcggacgtt cgatcaaaga atattcattg aatgctttat taggatcaat
    44401 cggctatgtg cctcaggatc acttcttgtt ttcgatgacc attcgagata atatccgttt
    44461 ttctaatcca aaactttcag aagaagaagt agagcatgcg gcttctttag catttatcaa
    44521 caatgaaatc aaggaattac caaaaggtta tgatacctta gttggagaac gtggagtctc
    44581 tttatctggc ggacagaaac aaaggatttc gattgctcga gcattgatca aaaatcctga
    44641 attattaata ttagatgatg cactttctgc agtagatgcc aaaacagaag aagctatctt
    44701 gcataattta aaagaaataa gaaaagagaa aacaactatc attgcagctc accgtttaag
    44761 tagcgtgatg catgcaaaag aaatcattgt tgtcgatcaa gggaaaatca ttgaacgagg
    44821 aacccatcat gagctgcttg cactaggcgg ctggtatgaa caaatgtggg agaaacagca
    44881 attagaagcg aagatagaag gaggcgaggc ctgatggatg aaaattatca atcggaatgg
    44941 acaaaatcga ttccacttaa agaacagaaa aagatcatca aacgactgat gaagttcgcc
    45001 aaacctttta gaacaacgtt catcgttgct atcttatttg cgtttgctct gtctgtgatc
    45061 aatgtcctat tgccaaggat catccaaact tttatggatg atcatctggc taaacagtcg
    45121 gctacgacac aagttatttt gttttttgct ggtatgtact tatttggagt cctagtaaaa
    45181 agtatcattt ggtttttcca atggtatttg tattccatgg cttcattgaa gacctatcag
    45241 tatattaggg taaaattatt tgaaaagctg catacactag gtatgcgtta ttttgatcag
    45301 acaccggcag gatcaaccgt ttctcgagtg acgaatgaca cagaaacgct atttgaattt
    45361 tggtacgtct ttttgatggt attgactggg atctttgcag tcgtttcttc attcattgcc
    45421 atgtttcaaa tcaatggaga aattgccttg tggactttgg tgttcttgcc gattttagga
    45481 attgtcatct ggtattacca gaaatttagt tctcgtattt accgaaatat gcgggaaaaa
    45541 ttaagtcagc tgaatacaaa attgaatgaa tcgatttccg gcatgcgtat tatccagcag
    45601 tttcgccaag aagctcgttt agctaaagaa tttgaacaag tgaacaatga gtatttagac
    45661 acgcgctatg cgatgatcaa aacgaattcg attctgttaa gtccgatcat caattttcta
    45721 tatgcattgg cgattgcgtt ggcgttgact ttatttggga tcgatgcgtt gcattctccc
    45781 gtagaagttg gactgatcta tgcatttacg acttatgtcc agggattttt taatccaatg
    45841 tctcaaatga tggacttttt gagcatcttc acagatggga tggttgctgg tagtcgtatc
    45901 ttgaagatta tggatacgga agaatatgca ccacaacaaa accctggagc agatcaaatg
    45961 attacttcag gtaagattga gtttcgcaat gtaagctttt cttatgatgg agagcacaat
    46021 gtattaaaca atatctcttt tgttgctaat ccgggagaaa ccgttgcatt agtgggacac
    46081 accggaagtg ggaaaagctc gatcatcaat gtgttgatgc ggttttacga attttatgaa
    46141 ggtcagatat tgattgatga tcatgacatt cgagaatatc caatggaaga gttacgtaag
    46201 aaaatggggc tggtcttgca agatgctttc ttgttttatg gggatatcgc aggaaatatt
    46261 cggatgatga atccagctat cacagacgaa cagatacgcc atgcggctga gtttgttcaa
    46321 gcaaacaaat tcatcgatga attaccaaaa ggatatcact caaaagtgat cgaacatggt
    46381 gccagctatt ctagtggaca acgacagctg atcacttttg cgcgaacaat cgtgactgat
    46441 cctaaaattc tagtattgga tgaagcaaca gcaaatatcg atactgaaac agaaggattg
    46501 atccaagaag gattggcgaa aatgcgacaa ggacggacaa cgattgcgat tgcccatcgg
    46561 ctgtcaacga ttcgagatgc agaattgatt ttagttttag acaaaggtca tatagtagaa
    46621 cgcgggaatc atgaaacact tctagcgcaa aatggtttgt atgcagatat gtacaaactg
    46681 caaagtagtg gtatataaaa gatgttgtga cagtagcatt ttgacctaag cactagagtg
    46741 aaaataaaac atagcttccc atatttttca tctgtgagag ttagtgtcgt agttaccaga
    46801 tttaaaaaag acgacgtcag ctagtcagaa gtgcctaata gcccctaaga aagcgttgac
    46861 aaaatcgttt gctttcgata atataaagaa aacgatacaa cttggatagt gattgcctga
    46921 tgcaagctaa acctgatgag tctcccatgg tttcatggac ggctttttgg gtttttcttt
    46981 ttgttttgga tacagcccta tatatcatgg aaaggagcaa aacatgctga ttgagaaagt
    47041 actgaataac aacgtcgtaa taacaaggga tgaaagagat cgggaaatga ttgtgatggg
    47101 aaaaggattg gcattcaaaa agaaggtggg tgacccgatt cttgactcat gcatcgacaa
    47161 aaaatttcag ttgacaaatg acggactttc acaacagttc caagaattgg cacaagcgat
    47221 tcctcttgag tacttagaaa tttcttgtga aatcattgaa tatacaaaag aggctctgca
    47281 agtccattta aatgattctg tgtatatttc attgacggat cacttgtata cagctatcga
    47341 acgtcaaaaa atggggatct ttgtgccaaa tgtattgctg tatgatatca aacaactgtt
    47401 tcctaaagag tatgccattg ggaaaaaaac agtgaagaaa atctcggata aataccacat
    47461 caagttatca gaagacgaag ccggatttat cgcattgcat ttagtcaatg cacaaggcat
    47521 tcaaagccag caagacaata cctacctgtt taccacaaca atacaggata ttttaaatat
    47581 cgtccgctat tactataaga ttacatttga tgaagattca gtccactttt ttcgattcgt
    47641 cacacacctg cgtttttttc tgacacgtgt cctagatgga gcgaaatcag aagatggaga
    47701 agtttctgag gaattgttcc aagttattca aaaaaaatat aaggatacgt ttggttgcgt
    47761 tgagaaaata gcagactttt taaccggggc tttcgactac cagatgtcca cggatgaaaa
    47821 aatgtattta acgatccata ctgcgcgttt agtgcatgag aatcgttgta aataaataaa
    47881 aaaggaggac aaattaacaa gaacgtatga aataagggta gtgacattca gttggctaaa
    47941 ccttccacta attaattgga gggaatagaa atgggaaaat accaggaatt agcagaaaac
    48001 atcgtaaaga atatcggagg aaaagaaaat atccgtagct tgacacactg tgtgacgcga
    48061 cttcgtttca aactaaagga ggaaaataaa gcaaaggatg atgttttgaa aaagatggaa
    48121 gggatagtca ccatcatgaa aagcggcggg caatatcaag ttgtaatcgg gaatcatgtg
    48181 ccagatgtct atgcagaagt actgacagta gccgggattt ccgatgaagc agaagaagag
    48241 gtttcttcaa gtagtaatct gttcaatcga ttaatcgaca tattaagtgg ctgtttccag
    48301 ccttttttag gtgctttggc tgcagcggga atggtcaaag gattaaatgc tttgttcttg
    48361 tttctcaaat tttatactga aggatcgggt acgtacatca tgctgaatgg gataggggat
    48421 gcaatttttt atttcatgcc agtgatctta gggtacacag cagcgaaaaa atttcatctg
    48481 aatccgatgg tggggctcgt gattggtgca gcactttgtt atccgactgt acaaggagca
    48541 gcactacaaa gtggtttccc tgatggcagt cctgcgcctt atagcttgct gggtcttccg
    48601 gcttatgaaa catttttggg tatcccatgg gtgggcagca gttatactag tagcgtcgta
    48661 cctgttatct tcattatcgc atttgccgga caagtccaaa aaattgccaa gaaatggatt
    48721 cctgcagtcg tccagacctt tttggtttct ttttctgtgt tattaatcgc acttcctgct
    48781 ggctttttga ttatcggtcc gattgtcagt atactgacag atctattaag cacgggattc
    48841 caagcattga tggacttttc tccagctgtg tatggtgcag ttctagggtt tctttggcaa
    48901 gtactggtga ttttcggact tcattggagt attatccctc ttgcgattat gcagctagcg
    48961 caaaatgggt ggagtcagat gttagtaggc gtttatggcg cgagttttgc acagacagca
    49021 gtggtattgg cgatgtattt caagttcaag gatcaaaaaa tgaaggcgct ttgcatgcca
    49081 gctgtgattt caggaatctt cggcgtaaca gaaccggcta tttatggaat caccttaccg
    49141 aagaagaagc catttattta ctcgatgatt ggtggtgctg tttcaggaat ctatttgatg
    49201 gcatctggaa caatcagcta cataatgggc ggattaggga tttttggggt ggtaaactac
    49261 atcacggaaa cagacgccag tggtatgttt caagcgttta tcggcattgc tattgcttcg
    49321 gtaatcgggt tttcgctgac ttttttcttt tggaaggatg aagcaggtga aacagatgct
    49381 atacagaaaa aatcaggtaa agaaattgta aaaacaccga tacaaggaca aatcgctcca
    49441 ctaaatgcag cgaaggatgc agcttttgca caaggaacac taggtcgagg aatcttaatc
    49501 taccctgaaa aaggagaagt acgtgcccct tttgacggaa cagttatgac cctgttcccg
    49561 acgaaacatg caatcggaat ggtttctgaa acagggctgg aattgctgat tcatgtcgga
    49621 ttggatactg tgcagttgga agggaaatat tttgaaagct tagtgcagca aggagaaaga
    49681 gtgagacaag gcgatctgtt gctacgcttc gacaaaaaga aaatcgaaaa ggaaggatac
    49741 agtttagaaa cacctgtgat cgtcaccaat tacacggatt atttggatat tttggaaaac
    49801 caaggggaga cggtcgggcc agacgatgca cttatcaccg cactgacctg aacggttagg
    49861 tgaagaatgg actaggctta actattcttt ttattccact tctttatttt caaaattgaa
    49921 aggctctttc atgatggctg agctgatagg gcgtttatcg aatttttgac cgacagaaat
    49981 tagatggact aaattctgtt gataattttc tgcaaaaaca caactatata acacgtctag
    50041 aagataacaa attgatgtat tgatcgtaaa attaccaatt tttgaataca gtcgctcgcg
    50101 tgtcgtaagt cgcaaagcgg ctgtactcat tgcagatagc gttgaatcac cgatactggt
    50161 caaagctaaa attggaacat cattttgttt caatatttct gcggtacgaa ggatcacatt
    50221 gttttcaccc gtataagaaa tcagaatccc gcacatatcg ctcgtacaat tgtacgcttc
    50281 atagaatgat tcaccaaaag tggtgacaat tgtgacatcc attttgatgc gcttcatttt
    50341 cagcatgaaa tcttgagaaa tcaaagtatt cgagttactg gcgaagatca cgattttttt
    50401 tgcctggagc agtaaatatt tagctttttg cagttggtca tgatgaatga gagaaagtgt
    50461 atcatgaatc gtcatctgtt ctaaagaagc cattttattt gcgattgtca taataccgtc
    50521 tgtggtggaa aaaggaagat tcgcatctac gtcttgaaaa ttcccttcaa gataggctgt
    50581 ttcagctaaa aaagcattac gaaaactgac ccagccatca aaacccattt tttttgctac
    50641 acggatgagt gtagacgggt ggacataaca tcgctcagct atcgcttgga tggtcatttc
    50701 gccaagtgct ggagaatcgc ttaagagaaa ctggatcact tcattttctg aaggagaaaa
    50761 ggagatttct ttgatttttt cacgtaataa catgtagata ccttcttctt atttttttac
    50821 gtaaattata acttttttga aaatgttttg taaacggatt tatactatat aacttaaaat
    50881 aggctttttt tgacaagata tttacaaaaa tccactagaa aacagtaact tttgtaaatg
    50941 ttactggaaa gaaacggatt tctattgttt gttttgttta caaaaaagat aatgaaagtg
    51001 taaaaaagta aaggagtgag aacatggcaa aaggaatcaa aatcgctacg attggtggag
    51061 gcagcagtta cacccctgag ttgatggaag gatttatcaa aagatatgag gaattaccga
    51121 tacgagaaat ctggctcgta gatgtagaag aaggaaaaga aaagcttgag atcgttagtc
    51181 agatgtctca gcgtatgtgg gatgcttctc catatgaagt caagatctat gcaactctgg
    51241 atcgggaaga agcattgaaa gatgcggatt ttgttacgac tcagtttcgt gtcggtcaat
    51301 tagatgcacg aatcaaagac gagcggatcc ctagttatta tgggatgatt gggcaagaaa
    51361 caaatggtgc tggcgggata ttcaaagctt ttcgtaccgt tccagtcgtt ttggcaatcg
    51421 ttgaggacat gaagcgttta tgtccaaatg cttggctgat caatttcgcg aacccaagtg
    51481 ggatggtgac agaagcagtt atcagatatg gcaaatggga taaagtgatt ggattatgca
    51541 atgtgccagt gatggcgatg atgacagaac cagcaatgct tggcaagaaa aaagaagagc
    51601 tgatctacaa gtttgccggt ctaaatcatt tccattggca caaagtagca gataataaag
    51661 gaaatgaccg cacggcagaa ttgattgatc gtttatacga tgaagataat ggattaccga
    51721 aaaatatctt tgatatcccg tttttcaaag aacaattaaa acaaatgaac atgatccctt
    51781 gtggctacca ccgctattac tatcgtgaag aagaaatgct ggaacatgct ttagaagaat
    51841 atcaaacgat tggtacaaga gcgcagcaag tgaaagaaac agaagcagag ctatttgaat
    51901 tatacaaaga tccagaatta gaccataaac ctgaacaatt gcaaaaacga ggtggcgctt
    51961 attattcaga cgctgcctgt gaaacgattg cttcaatcta tggaaataaa gaaacacaga
    52021 tcgttgtatc tacaaagaat gatggagctg ttcctgacct gtcggcagat tgtgttgtag
    52081 aagtcactgc ctatgttggc gcacaaggag cgagaaatgt agcgtttggt tctcttcctg
    52141 ctgctgaaag aggctggttg caagtaatga agaatatgga attgctgacg atcgaagcag
    52201 ctgtgactgg tgattatggg acagctttac aggcgtttac gatcaatcca cttatacgat
    52261 caggaaagac agcaaaacgg atcatggatg agttgtttat tgcacaccag cagtatttac
    52321 cgcaattcaa ggaaacaatc agccgattaa aagaagaagg aatcgaagtt caagatgaac
    52381 tggcaagaga attatcaggt aatacacata taatggagga agtataatgg ggttcagaaa
    52441 agatttttta tggggcggtg ctacagcagc caatcaatgt gagggcgctt atgatgaagg
    52501 tggacgcgga ctagcaaacg tagatgttgt gccaatcgga aaagaccgtt ttcctgttat
    52561 caccggaaag aaaaaaatgt ttgaatttga tgaggatcat ttttatccag caaaaattgc
    52621 aatcgatatg tatcatcgtt ataaagaaga tattgctttg tttgcagaaa tggggttcaa
    52681 gacttatcga atgtctatcg cctggagtcg tatttttcca atgggggatg agaaagagcc
    52741 gaatgaagct ggcttgaaat tttatgagga tgtctttaaa gaatgccaaa agcacggaat
    52801 tgagccactt gtgacgatta cgcattttga ttgtccaatg catttgatca aagaatatgg
    52861 tgcgtggcgt tccagagaac tcgttggttt ttatgaaaat ctgtgtcgag tcatcttcaa
    52921 tcgctataaa ggacttgtca aatattggct gacattcaat gagatcaata tgatccttca
    52981 tgcaccattt atgggcgcag ggctgtattt tgaagaagga gaaaatgaag aacaaatcaa
    53041 atatcaagcg gcacatcatg agttggttgc aagtgccatc gcaacgaaga ttgctcatga
    53101 agtagatccg gagaacaaag taggctgtat gctggcagca ggagcatatt atccatacac
    53161 ttgcaatcct gaagatgttt gggcttctag aaaatcagat cgagaaaatt atttcttcat
    53221 tgatgtccag tcacgaggag aatatcccgc ttatgcattg aaagaaatgg agcgaaatgg
    53281 cattcagatc gaaatggaac caggggatgc cgcattgctg aaagaacata ctgttgattt
    53341 tatctctttt tcttattatt cttcacgagt ttcgactacc gacaacgaac tcttggaaca
    53401 gactgcagga aatatctttg cttctgtcaa aaatccatat ctgcaagcaa gtgaatgggg
    53461 gtggcaaatt gatccattag gcttgagaat aacgatgaat gatatttatg atcgttatca
    53521 aaaaccatta tttattgtag aaaacggctt aggtgcagtc gatacgccag atgaaaatgg
    53581 ttatgtggaa gatgattacc gaatcgatta cctggcagcg catatccagg cgatgaagga
    53641 tgcagtagaa ctagacggag tcgatctatt aggctatacg acttggggat gtatcgattt
    53701 agtatcagct ggtaccggtg agatgaagaa aaggtacgga ttcatttatg tcgatcgtga
    53761 taaccaagga aatggcacat tagcccgttc aaagaaaaaa tcattcaact ggtataaaaa
    53821 agtaattgat tcaaatggag aaaatctttc gaacaattga tcaatgattt ataaaagtct
    53881 attgtttttc agcggatttt tcgagtataa taaagctatt cgagaaacgc aagcagaaca
    53941 atagactttt tttcgcattg gctgtaaagt atgcttactt gattaagtaa aggagaatct
    54001 acatatgtat gatgtaatcg ttattggcgc aggtcctgct gggatgactg ctgctttgta
    54061 tgcttcacgt tcaaacttat ccgtattgat gatcgaacaa ggtgcccctg gcggccaaat
    54121 gaacaatacg gcagaaatcg aaaattatcc tggattcgat tcgatcatgg gccccgatct
    54181 agcttataag atgtatgaaa gtgtctcaag atttgggaca gaaaatgctt atggaatcgt
    54241 tcaagatatc aaggatcacg gcagccataa agaagtcatc tgtgaagata aaagttacga
    54301 aggaaaaaca gtcattatcg caacaggctg tatccaccgt aaattaggcg tacctggcga
    54361 ggaagaattt gctggacgag gcgtttctta ctgtgcagtc tgtgatggtg cgtttttccg
    54421 taacaaacgt ctagtagtca tcgggggtgg ggactcagca gtagaagaag ccatctattt
    54481 gacacaattt gcttctgaag tagtgatcgt ccaccgtaga gatgaattac gtgcacaaaa
    54541 gatcatccaa gaccgtgcat ttgcaaatga aaaaatttcc tttatgtggg atacagtcgt
    54601 tgacgaaatc gttggaaacg aaatggtagt gacaggtgtg aaagcacgta atgtcaaaac
    54661 cggcaatgaa tttgatttag cagcagacgg agtattcatc tatgttggac tggatccatt
    54721 aactgcacct tttagagaag caggtttgac gaacgaagct ggctggattc caacagacaa
    54781 cgaaatgaaa acgaatatgc caggcgtttt tgccatcgga gatgttcgtg aaaaagattt
    54841 gcgacaaatc actacagctg ttggtgacgg cggtattgct ggacaacaag tgtataaata
    54901 cttggagtct ttagaagaaa caacagcata agtttaaaaa gagtgaaaga acggtttcaa
    54961 ttgttctttt actctttttt ttcgcgtgaa atccactgaa tccgatttat gtgaatactt
    55021 gcaatcatgc ttgataaaag aatcctttct tgatttaagc gttttttggt cctcatactt
    55081 tcatatctaa taagtgaatg ctatacttat taagaaccat atttcagaaa gaggtgttat
    55141 tttcatgtct tgggaacaag tgtatgaaca atgggtacat gaagaaaatt tagaagaaaa
    55201 cttaaaaaaa caattaaatg atctgggtga agatcctgaa aaacttgaag atgcttttta
    55261 tgcaccgttg gaattcggta ctgcaggcat gcggggaatc ttaggacctg ggatcaatcg
    55321 aatgaacatc tatacggtaa gacaagcaac agaaggacta gcacgtttca tggatacaca
    55381 aggtgaagaa acaaaacgcc gaggtgtcgc tatcgcttat gactcacgcc atcagtcacc
    55441 agaatttgca atggaagcag ccaaaacact ggcacatcac aatatcccgt cttatgtatt
    55501 cgaaagtctg cgtccaacac cagagctttc ttttgcagtt cgtcatttga agacgtttgc
    55561 gggaatcatg gtaaccgcat ctcacaaccc agcggcttac aatggatata aagtctacgg
    55621 agaagatggc ggacaaatgc ctcctgcaga cgcagatgca ttgacgaaat atgtccgtga
    55681 agtcagcaat ccgctaaaag tcgaagtatt atctgacgaa gaagctaaac atagcgggtt
    55741 gatcacgatc atcggagaag aagtggacgc tgcttatcta gaagaaatca aagcagtgac
    55801 gattgatcgt gaattggttg aaacaatggg gaaagatttg aaacttgtgt acacgccact
    55861 tcatggaaca gggaaaatgt tgggcgaacg tgcgttgaaa caagctggat tcgaacaatt
    55921 cgtactagtt cccgaacaag ctgtggcaga tccagatttc actacggtca aatcgccaaa
    55981 tccagaagaa cattcagctt ttgaatatgc tatacgactt ggagaaaaag agggagctga
    56041 cttgctgatt gcaacagatc ctgatgcaga ccgtttaggc gcagcggttc gtttgccaga
    56101 tggttcttat gaagtattga ctgggaacca aatcggagca ttgtttgctc aatatatttt
    56161 ggaagcacat caatcaagag gcactttacc agaaaacgca gcggtattga aatcaatcgt
    56221 ttccagcgaa ttgcctacag caattgcgga acactacggt gctactatgt tcaatgtgtt
    56281 gacagggttt aaattcattg ctgaaaaaat ccaacaattt gaagaagacc attcacatac
    56341 ttttatgttt ggattcgaag aaagttatgg ttatttagtt aaaccttttg ttcgcgataa
    56401 agacgcgatc caagcattgc ttttattagc agaagtagca gcttactata aaaaacaagg
    56461 caaaacgttg tatgacggct tgcaaacgat ttttgaaacc tatggctatt tcgcagaaaa
    56521 aacgatttct gtgacaatga gcggacaaga aggttctgcg aaaatctctg ctttgatgga
    56581 aaaattccgt aaagaagcac cgacagaatt tgccggagca aaagttattc aaacagaaga
    56641 ctttaaagaa ttgacgagaa cagaccgtga aggaaacgta gaaaaactaa caacaccgcc
    56701 atccgacgta ttgaagtata cattggaaga aaatggctgg atcgctatcc gcccttcagg
    56761 aactgagccg aaaatcaagt tttatattgg tgtaaaagga agctctacag aagacgcagc
    56821 cgaaaaagta gcctcttatg aagcgattat caaaaagatg acagaagaat aaaaaacttg
    56881 agactgatgg aaaaagtggt aaactttaga agtcagtctt ttttcaagtt ggaggtgaat
    56941 ggattgccag acacgcaaaa atcaaaagta gagaaagtca gtcaattatt caaaatgttg
    57001 agtgacccca ctcgtcttaa aattctgcta tatctgaaag acggggaaca aaatgtgaca
    57061 gcaatcaccc aagcagtgga aatggagcag tctgctgtat cgcatcaact acgtttatta
    57121 agagaaaatc atgtcgtcaa gtctcatcgc gagggaaaag cgattttgta tagtcttgat
    57181 gattctcatg tactggatat tttgaaccag acactcaaac atgtggaaca tcaataacga
    57241 taggtgcaaa tagataaaca ctggagtgtg acaacgaaat ttcttcgttg tcacactcca
    57301 gtgtttttta agaaaaagac aaaaatgatt aatcgctttt ttctgttgtt tcagttccat
    57361 aataactatg atcttctagg tctaaagcat tcaaaataag cgaagccgtt tcttcaatag
    57421 ataaagatgc cacattgatc actacacatc ctagtttttc ataaagatca ttcgcaaaat
    57481 caagttcagc tctaattttt tcgatatcgg aataagcagt atcagggttt aatccgtaag
    57541 agcgcatccg ttctttacgg atattattta aaatatctgg gttattcgtt agtcccacga
    57601 tttttttagg atcgatctcc caaagctgtt tgggaatatg tgcttgtggg atcaaaggta
    57661 aattggcaac ttttaaattt ttgttagcta aaaacaagct caaaggcgtt ttggaagttc
    57721 gggatacgcc tagcaaaacg acatctgctt ctaaaaaacc acgaggatct ttcccatcat
    57781 cgtattttac agcaaattcc attgctttga tccgtttgaa atagttctca ttcaataaat
    57841 ggagggcacc aggttgccga attggcttga taccagttaa acgttcaatt tctgatatcg
    57901 gtggcgtcat cacatcaaaa ctaaataatt gatgttcttc acaaaaagaa cgtgttatct
    57961 cgatcagttc ttgattgatt aatgtatgca ggacaagtgc tttttcttct aatgcatctt
    58021 gcaatgcctg ccttaatgtt ttttcatctg tcacgaacgt tctgcggaat aggttgaact
    58081 caacggtagg atactgagcc atagtagctt gcgctagttt tgtcgcagtt tctccagcag
    58141 agtcggagat gacaaaaaaa gtaacgattt ctgtagaatc ggaagacatg gataaaacct
    58201 cacttgcatt ttttttatgt ttattatacc atactctaga atgcgtactt cgtaatcatt
    58261 ttgatttgga ggaataagtc atgaaaaaac agacattgat cgaacaatca ttagaaacat
    58321 tgaaaaataa cggttttaaa tatactaaaa aaagagaagc attgttaact tatttagtga
    58381 agcgtaaccg ctacgtatct gctaaagaag tttttgacta tatgaatcag gaattcaaag
    58441 gggtaagtta tgatacgatt taccggaatc tccatgattt tgcagagatc gatttactgg
    58501 aagaaaccga tttaaatgga gaaatgaagt ttcgttttca ttgctgtcag gggaaaatcg
    58561 gccatcacca tcattttatc tgtacagtgt gcggaacaac gaaagaaatc catatgtgtc
    58621 cgatgaatta tttcgaagaa cagctaggtg gatgcacgat cgaagggcat agattcgaga
    58681 ttttcggaag atgtgaaaat tgtcaataag gatttaaata aaataacatt tgataatttt
    58741 aaaaaaaagc aagatttcct tgacggtcaa aacagcatta gatataatat ttacaggaca
    58801 gtcttgtttt ttgagatata tttcaataac aattacggtt actttttaag ctcggaggga
    58861 gggaattaac atgtcaaaaa cagtcgttcg caaaaacgaa tcacttgacg atgctcttcg
    58921 tcgcttcaaa cgttccgtgt caaaagcggg tactttacaa gaatctcgta aacgtgaatt
    58981 ctacgaaaaa ccaagtgtaa gacgcaagaa aaaatctgaa gcagctagaa aacgcaaaaa
    59041 attctagttc tgtgatgaat tggagttggt agtgtgtcac tactaagtag attaaacgat
    59101 gacatgaaag cagcgatgaa agcgaaagat aaggaatctt tacaagtcat ccgaatgatc
    59161 aagtcatcta tacaaaacga gcagattaaa gaaggtcacg acttgacaga agaagaagaa
    59221 ctaacggtgt tgtctcgtga aatgaaacag cgacgtgatt ctttgcatga gtttgaagaa
    59281 gcaggacgtg acgatttagc tgaaaaagta aaaagtgaaa ttgtaatagt cgaaaaatat
    59341 atgccggaac aactttccga cgaagaaatt cgacaacttg ttcaagaagc aatcactcaa
    59401 actggtgctt catcaatgaa agaatttggc aaagttatgg gtgcaattat gcctaaagtc
    59461 aaaggaaaag cagatggaaa ccaagtgaat gcgattgtga aagaactatt acaatcttaa
    59521 ttttatcatt taaactgcgg acagtgatta tccgcagttt tttgtgttcg taaaaaaaga
    59581 actaaaaaac agctagaaga aagaatctta ttctttcttc tagctgtttt tttcctgttt
    59641 aatatctctt atgttaaatt gtgtttgatt tggttattct tctggtaact cacttgcttc
    59701 ttgtttatat cctgatacgc gatatacata ataaaagagc aagctgccag cgtaaccaag
    59761 caacgttggg acgacccagc caagacctaa tgcagcaaaa ggaacataat gatgataaaa
    59821 tcctagtaag cttgctacga cagtttgctg actcactaat gcaggcgaat ttgccaacat
    59881 atccaatacg gctggaacgg cagaaaagaa catcgttact tgatagacaa atggactctt
    59941 ttggaagaat tttgctgtta aggaaagtaa gattaacgca agtgacaatg gatacaaaag
    60001 catcaatact ggtaaagacc attggatgat attatccaat ccggcattcg caacgacaaa
    60061 agagacaaaa gtcgttaacc gcagccatgt catataactg actttaggga acagtttatg
    60121 gaaatcttgt gcaaaagaaa caacgagtcc catagcagta gtgaagacac ctaatgtgac
    60181 cattacccct aagaagctag caccgaagtt acctaaataa aaattagtga tctgagaaag
    60241 tgcagttccg ccattttctg ataaagacat cttattcaaa ctcatcgcac cgagaaggac
    60301 cagaccaaaa taaatcatga cttctaacgc aacgctcagg ctaccagctt ttgctgttaa
    60361 ttctgtgact tttttatctt taaaaccaag atcacgaact gcatggacaa aagtgacact
    60421 taaagcaagt aaagctactg catcaaccgt gttgtatcct tctaaaacgc catttgtcaa
    60481 agcgttcgtc tgataagaat ctgcagcaga ttggtttaga ttccccatgg gttggaataa
    60541 agaaacgaca aaaatgatag ctaataatgc aagaaacaag gagtttaaat actttccgac
    60601 atatttgacc aaacgtgttg gatgtacagt aaggaaataa gcaagtaaaa agaaaagagc
    60661 agagaataaa aacataccta atgaatcata tttttcaggc aaaaagggtt tagcagccat
    60721 ttcaaaagca gttgctgctg tccgcggtgt accaaagaat ggtccgatcg ttaaatgaac
    60781 aaggatcaag aacaaggcag cataccattt tccgacaggt ttagctaagt catacagacc
    60841 atcacttttt gtcactacta cagctaagat agctaataat gggaaaagag cgcctgagat
    60901 ggcaaatcct acaccagcgc tgatccagtt tgatccagcc atttgaccta agtgtacagg
    60961 aaagatcaga ttacctgacc cgaagaacat cccgaagata agtgaactga ccatcaataa
    61021 ttgcttccat gaataagttt ttgtttcttc cataaatttt tcctccaata attatcattt
    61081 tgagtaacaa aataaacgtt gcagaaacta gaaataccac taatatcaga ttccatgaaa
    61141 accaccacct taaaatataa aaaaagccct tttccacttg ggaaaagggc tctagattct
    61201 agaacggtac cacctttttt cgcttaaaaa agcttcttca ttcacggacg cttttcgata
    61261 aagttatccg ctagcctgat aatggaggca gccacaatga tctacgtttt cgacattgac
    61321 tcggaaatga tgttcgtcta ttcgatttat tgtcatttct cacttacatg actcactggg
    61381 aataaatcac tgaatagcct actgttttcc tcgacgcttt gctgtatatt tatttgttga
    61441 cgttataata cacctgtttt ttttgaaatg caagaatatt tcaaatattt tgttttcttt
    61501 ttttgaaaca agaaaaatac ccagagaata cttgctaagt tagacggaat acttgtcctc
    61561 tatcttatgc atagcttaca tgatcagctt tttttattca aacgagacct ttagagaatg
    61621 gaagcggcta gttttttaac gtttcttggt acttatggtt aagcaactct tttagaacct
    61681 tttcttattt gctatatagt ttcttctgag tatggtaaaa taggaaagaa tgaatcgaaa
    61741 aaaaggggat gtctattttt gacggaagag tctagttctt tagagattag attgacggct
    61801 gctgatgatg tcagcatgct tttaggtgca cacgacaagc atataaaact gattgaggaa
    61861 acaacggaaa caaccatcca tacacgtgga gaaatcattc agatcattgg tgagcaatcc
    61921 cagatttcct tggctgcctc agtcattcgg acattacagg aattgatcaa acgaggaatc
    61981 cacgtaagta cgcccgatgt tgtgacggca ttgaaaatgg ggcaaaaagg tacattagat
    62041 tattttgttg agatgtatga ggaagagatc gtcaaagacc gtaatggaaa acctatacgg
    62101 gtaaaaaaca gcggccaaaa gaaatatgtc gaatcggtag ccaaacatga tatcgtattt
    62161 ggcgtaggac cagccggtac aggaaaaact tttcttgcag tagtattagc aatcgctgcc
    62221 ttgaaaaaag gtgaagtgca aaaaatcatt ttgacgcggc ctgctgtgga agcaggtgaa
    62281 aatctaggct ttcttcccgg ggatctaaaa gaaaaagtgg atccttatct gcggcctgtt
    62341 tatgatgctt tataccaaat ttttggattg gatcatacga accgattgat ggaacgaggc
    62401 gtaatcgaaa tcgcaccttt agcttacatg cgggggcgaa cattggatga tgcattcgtt
    62461 atcttggacg aagcacaaaa cacgacagtc gctcagatga aaatgttctt gacccgtctc
    62521 ggttatcaat caaaaatgat tgtcaatggg gatacaagtc agatagactt gcctcgaggg
    62581 acgacaagtg gtcttgttca tgcagaacga acattaaagc aaatcaagaa aatcgatttt
    62641 gttaattttg aagcaagtga cgttgttcgg caccctgtag ttgccgaaat catcaaagct
    62701 tatgaaaagg cagatctcca tcaagaataa gctttgctta ggaggataag cacatgttga
    62761 ataaaacact gcagaaacta caagaaaaac ttggaaaggc agcattgcct ttcctgctgt
    62821 tcttgttttt tatcattatc tgcggcgtta catttagtag tgtcaaacaa aaggccaatg
    62881 atttcagaga aggccaagta gcagaagaaa gtatccgtgc gaacaaaacg atcgagaaca
    62941 cggaagaaac cgaacaaaaa agaaaactgg cagcagaagc agtcactcca gaatacactt
    63001 accaaaaaga tcttgcagat gaccagaaca atcggatcaa gcaactattt gaattgatcg
    63061 ataagacgaa tgatgccatc aacaaatctt atgacgaaaa gattgataag gcaaaggaag
    63121 atgaaaatat cccaaagcct actacagaag agcggatcgc cgcgctgaag aaaaattttg
    63181 aaaatgtgaa tgcagaaaat gttgtgtttt atcaaaaact accgaacaat ttttattcga
    63241 cgatatttga aatgactgaa actgagattc atactgtgag ggatgagagt ctgaagctca
    63301 ttgacgaaca gatgagcaaa caagttcgtg aaagtgaatt ggaagctttt aaacaagaag
    63361 cagaagagca aatccaatat ttgaatgtca caccagcaca acaacaaatg attcgctact
    63421 tagtggatca aggtatcgta gtgaatgacg tattgaatga gaaaaagaca gaagaattga
    63481 aacaatccgc acgagaagct gtacaaccag tgatgatctt ccaaggagaa atcattgttc
    63541 gtgaaggtaa tcaaatcgat gcttcagcta tgaaaaaatt ggaattacta ggactgacta
    63601 atcaaacaac ttcgattttt ccgctaattg ccatggtttt agctgttttg cttcaaatcg
    63661 gcgtactgat ttataattca cttcaattcc aaaatgcagg agaaagaaca aaatacgtgc
    63721 ttttctatgt gacagctatg tcagtcagca tagtgctgat gaaatttttc caattattcc
    63781 aaacggaaca agctacttat atcccgctgt tttatcccgc tgcttttgca ccacttgttt
    63841 taagcttttt cttgaatcga agagcaggga tcatggctgg tattttccaa gcagtatccg
    63901 cacttttcat tttttatgat tcgattggca cgaactttct aacgattatc ttgatgtctt
    63961 atgttttctc tgggctcatg gcaacgattg tcagacgcaa acggatctca gaacaaggat
    64021 tatctgctgc catgtgggtc attatttttc ctgtgatgat ggatgtcatt ttgatcgtct
    64081 atcaaggaat gagctttggt gatgcaacga cttggttaat gctcgtctgc gggttcactg
    64141 gtgcactctt ctcattcttg ctgacgatcg gtctacaccc atatattgaa ttgatggtca
    64201 atgatgacag tgtaattgta ttaaatgaac tcagcaatcc aaatcatcct ttattgaaac
    64261 agttgctgga agaagcgcca ggtacttatc atcatagtat gatggtagct aatctaagtg
    64321 ctaacgctgt agcagaaatc ggcggacgtt ctttgctaac tcgagtagct tgctattatc
    64381 atgatatcgg caagatcaaa catgctagct ttttcgttga aaacttgcct tcaggagcgg
    64441 aaaacccgca caatttctta ttgccagaag acagtaaaca tattatcttt ggacatgtga
    64501 cagatggagc caaaatattg gaagattata atatgccgca aatggtgatc gatatttgca
    64561 aacagcatca tggaaccacg ctaatgcgtt atttctacat caaagcgaaa gaacgcaatc
    64621 cggaagtaac agaagagcaa ttccgttatc ctggaccaaa accgcaaagt cgtgaagcag
    64681 ggatcgtcaa tattgctgac agttgtgagg ctgcagtacg ggcaatggat catccgacga
    64741 gcgaaaaaat cgaaagtttt gtccataatc ttatcgaaga gcgtatttcc gatggtcaat
    64801 tagacgatag tggactgacc ttgaaagaaa tccgaaaagt ggaaaaatca ttaatcagcg
    64861 gtttaagtag caccttccat tctcggatca aatatcctaa aatgaaaaca gaagttgaga
    64921 agataaagga agaacaagaa gaatagaaag aagcgatgta taatggatat tacctttatg
    64981 gatgaaacaa accaagtctc agaagagaaa atgaaagaaa tcgatgactt gctgcaattt
    65041 gcagcatctt atttaaagct gccagaggaa acagaaatgt ctgttacttt catggacaat
    65101 gcggcaatcc aagtaatcaa tcgtgattac cgtggaaaag atgcaccgac agacgtcatc
    65161 agttttgctc tggaagaaga aggcgaagac gaaatcccag tgatttttga agatggagag
    65221 aatccattac ctcgtgtact aggagacatc atgatctcta ttgaacgtgc tgctgaacaa
    65281 gcagaagctt acggccattc atatgatcgg gaacttggct ttttagcggt acatggattc
    65341 ttgcatatca acgggtatga ccatatgaca ccagaagatg agaaagaaat gtttggttta
    65401 cagaaagaga tcttggatgc ctatggactc aaaagataaa aacgtagaga aaaacaaaca
    65461 tttcctgatg tctgtggaat ttgctgtcca aggagtcaaa accgtttttg aggaagaacg
    65521 caatatgagg aaacatgttg cgttcggtat ccttgcttta tttatgggcg tgattttcca
    65581 gcttgatcgg atagagtggt tatggctgct tttagctgtt tttctcgttt ggatagtaga
    65641 gatcatcaat actgtttttg aaaatgtagt ggacatgttt acggattttc attttcatcc
    65701 gattgggaaa aaaatcaaag atatggcagc tggagcagtt ttagtgaccg cttgttttgc
    65761 tgtcatcatt gggttgattt tatttgtccc taaaatatat catttgttgt ttaattaaaa
    65821 aggagaacgt atatgccaga acataaatca gggtttgtcg caatcgtcgg ccgcccgaat
    65881 gtaggaaaat ccacattatt gaaccgaatc gtgggtcaaa aaattgccat tatgagtgac
    65941 aaggcacaga caactagaaa taaaatccaa ggagtatata ctacaccaga agctcagctg
    66001 atttttatcg atacaccagg aatccataaa ccaaagcatc gtctaggaga ttttatggta
    66061 gaagcagctt acagtgcgtt gaaagaagtg gatgcagtct tatttatgat cagtgctgat
    66121 caaaaacgtg gccggggaga cgattttatt attgaacgtc taaaaaacgt gcattctcct
    66181 gtttatctga ttatcaataa aatcgataag atccatccag acgagttgct gggaatcatc
    66241 gaagattatt cgacgcagat gccttttgca caagtgattc cgatctctgc tacagaagga
    66301 aacaatgtcg aacgcttaat ggatgtatta gtcagtgaaa tgccagaggg cccacaatat
    66361 tttccagatg atcaagtgac tgatcatcct gaatacttca tcgtttctga attgatccgt
    66421 gagaaagtct tgtttttaac acgagacgaa gtgcctcatt ctgtcgcagt cgttgttgat
    66481 tcgatgaagc gaaacgagaa tgataaaatc catatccagg ccacgatcat cgtcgaaaga
    66541 gatagccaaa aaggaatcat tatcggcaaa ggcggaaaaa tgctgaagca agtaggaacg
    66601 aaagcaagaa aggatatcga aaatctttta ggagacaaag tatttctaga actttgggtc
    66661 aaagtccaaa aagactggcg agacaaaaaa gtctatctgc aagacttcgg ctaccgaaaa
    66721 gacgagtact gatattctgg acttcttata ggttaggtat agaaataaac tggataaaaa
    66781 tccgtacgtt gagaaaaaca gcggaagatc gaaacataaa taatctggat cggcagaatc
    66841 tgcttttcca gatttatttt ttaggaggga gaatatgagc cagcttgcag aatcgaaagg
    66901 cttgatttta tctagccgta attacaaaga aaaagataaa ttagtcaaaa tctttacgga
    66961 atcttctggc aaaatgatgt tttatatcaa aggaatccat cggcaaaatc agccattagc
    67021 tccggctttg ctccccttta ccgaagcagt ctatatcggt aattttcgaa gtgaaggttt
    67081 gtcgttttta agcagtgtca aagacgtcca tgcgtttcgg aacatccaag aagatatctt
    67141 tttgagcggt tatggtacgt atttgctaaa tctagtagat gcagcaatcg aagatcatca
    67201 atatgacccg catttattcc aatttacgca acaagctctg cagcggatgg accaaggaga
    67261 tgatgcagaa atcatcacga atatctttga agtgcaaata ctgcaacgct tcggaatcgc
    67321 gccaatttgg acgcactgtg tcgtatgcgg ggaaacaaaa ggaaaatttg actattcttc
    67381 caaatatggc ggtgtgatat gcgagaaaca ctggccaatg gacgaacacc gttatcatgc
    67441 agatcctcgc gctgtttatt tcgttcgaat gttttcggct atttcttatg ataagatcag
    67501 tggaatccac ttgaaagaag aaacgaagca ggcaatccgt gacctcattg accagttgta
    67561 tgatgaatat gtcggtattc atctaaaaag caaaaagttc attgatcaaa tgaagtcttg
    67621 ggcacacgta ttaaagacgc cagagaaaaa tatgcctgaa aaagagatac ctgaaaaaga
    67681 agaataaatg aaagatacaa caaaaaaatc aaaactattt gacaaataca gggacttccg
    67741 tctattatta accgtagatt aaatagatcg ttcactgaga ggatctatta tacaaaccag
    67801 aaaactgcaa aatgaatacg tacattgacc aagatcagta aaaaacacgt ggtttagcga
    67861 gttcgggaga gtggaagccg gacccaaacg atttttgaag aggcacttgc gagtacgata
    67921 aatgaagctg ccgaatcttt attcggaagt agggtggaac cgcgaaatta ttcgtcccta
    67981 tgtctgtaat cagacatagg gactttttgt cgttttcttt cctgtattct tattgacagc
    68041 aacgcttaaa aattgaaagg ggcaaaacag atgagtaaaa aattgaccgt ccaagatatg
    68101 attttaacat tacaaaaatt ttggtcatca aacggctgca tgctgatgca agcttatgat
    68161 acagaaaaag gagcaggtac gatgagccca tatacattct tacgggcaat cggaccggaa
    68221 ccatggaatg ctgcttatgt agagccatcc cgccgaccag ctgacgggcg ttatggagaa
    68281 aacccaaacc gtttgtatca gcatcatcaa tttcaagtcg taatgaaacc ttcacctgaa
    68341 aatatccaag agctttattt agaaagcttg cgtcttttag gaattgatcc attagaacac
    68401 gatattcgct ttgttgaaga caactgggag aacccatcga tgggttgtgc cggtgtgggc
    68461 tgggaagtat ggcttgatgg gatggagatc acacaattca catacttcca gcaagtcgga
    68521 ggactacctt gtaaaccagt tacttctgag atcacttatg gactagaacg tcttgcttct
    68581 tatatccaag aagttgaaag tgtttatgat ctggaatggg ctgatggcgt gaaatatggt
    68641 gagatcttca aacaaccaga atatgaacat tctaaatatt cttttgaagt aagcgaccaa
    68701 gacctgctac taagcaattt tgatcgtttt gaaaaagaag ctaaacgctg tatcgatgaa
    68761 aatcttgttc atccagcgta tgattatatt ttgaaatgta gccatacatt caatttatta
    68821 gatgctcgcg gtgcagtatc cgtaacagag cgtgcaggtt atctttcccg tatccgcaac
    68881 atggctcgtg ctgtagccaa aatctttgtg gcagaacgcg aaaaattagg attcccttta
    68941 ttgaataaaa acacagcaaa tccagaaaac gtgaaggagg ctgaataaga tggcaaaaga
    69001 cttattactt gaaatcgggc tggaagaaat acctgctcat gtagtcacac caagcatgaa
    69061 acaattagaa gaaaaaacag caaaattttt agatgaaaac caattgactt atgatacgat
    69121 cgaaacgttt tctacaccgc gccgcttagc aatcaaagta acaaacattc cagaacgtca
    69181 agcagacagc gaagaagaag tcaaaggacc agccaaaaaa attgctttgg acgcagatgg
    69241 aaactggacg aaagcagctg aaggatttgt ccgcggacaa ggtctgacga cagatgctat
    69301 cacttttaga gaattaaaag gcgtggaata tgtctatgta acgaaataca ctcacggcaa
    69361 agaagcaaaa gaagtattga caggtttagc agatgtcatt aaaagcctca ctttcccagt
    69421 aacgatgcac tgggcagaat acgattttga atatattcgc cctattcact ggatcgtggc
    69481 attgttagat gacgaaataa tcccgtttga gatcttggat gtcaaaacag acagagcttc
    69541 aagaggtcat cgtttcttag gcgaagatgt gacatttacg catgcaaaac aatacgaaga
    69601 aaaattgaat gaacaatttg ttattgcttc accgcaaaaa agaaaagaaa tgatcatcac
    69661 acaagcaaac gaattagcag aaaaaaatca ctggatcatt gatctagatg aagatttact
    69721 agaagaagtc aacaatttag tcgaatatcc aactgtcttt tctggcgact tcgaagaaaa
    69781 atatctatct gttcctgaag aagtactagt cacttctatg aaagaacatc aacgttattt
    69841 cgaagtacgg aacgatcaag gtatgttgct tcctcatttt atttctgctc gtaatggcga
    69901 tcgggtaaaa ttggaaaatg tggtacgagg caatcaaaaa gtattgactg ctcgtttaga
    69961 agatgcggaa ttcttttata atgaagacaa gaaagcttca atcgaccaat acgttgaaaa
    70021 attaaaaact gttactttcc atgaaaaaat cggtagcatc tatgagaaaa tgcaacgggt
    70081 acaagtcatc gctcgcatca taggcgaaca agtaggacta tctgaggaag aattgatgga
    70141 tctgcaacgc gctgctgaaa tctataaatt tgatctagta accaacatgg taggagaatt
    70201 ccctgaacta caagggatca tgggcgaaaa atatgctctg cttaaaggcg agaaaccagc
    70261 cgtagcaaca gcgatccgtg aacattacat gcctatatcc agtgaaggag atttacctga
    70321 gactacagta ggttctgttt tagcgattgc tgataaatta gatagtatct tttctttctt
    70381 tgcagttggg atgatcccaa gtggctcaaa cgatccatat gctttgcgcc gtcaagcata
    70441 tggtgtcgtt cgaatcatcg aatcgaaaaa atgggccttt cctttgtctg ttctgcaaga
    70501 aacgattagt gaagttatca gtaaagatac ggatcgtttt ggcattggct tgtcagcggg
    70561 acaacaacag gtgatcgact ttatcaaagg ccgtctgcgc caattattga caacgaagaa
    70621 tatccgtcat gatgtcatcg aagctgtttt gaatgcagaa caaaaagatc tgacaaaagt
    70681 atttgcagca gcacaattgt tcaagcagca tcttgcagat gaagacttca aaccatcgat
    70741 ggaagctttg acacgtgtcg tgaacttagc gaaaaaagcg gagcttgaaa aacaatcaga
    70801 agtagaccct gaactgttcg aaaacgaagc ggagaaagaa ctgcacaaag cagtagaggc
    70861 aatgaaaaca agctttgctg accaaacgat caacgaaaat tatgatcagc ttgtaagcct
    70921 tcgcccactg atcgaaaatt attttgatca gacgatggtc atggcagaag aaccagcagt
    70981 acgtaagaac cgattaacgc aattaagtca aattgctgcc atggctttgt cactagcaag
    71041 tttagatcaa atcatcacaa aataatttct aatatgtaaa taaaccagag aacaggcaca
    71101 aaataggcta tttctctggt ttatttgtta taaatataaa taactattat taattcaatg
    71161 ttaatttttt tgttatttta ttttttgtgt aacttttaaa aacacctatg aa
//
LOCUS       DANJMI010000009        66270 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000009 DANJMI010000000
VERSION     DANJMI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.009"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(264..563)
                     /locus_tag="PWU81_000714"
     CDS             complement(264..563)
                     /locus_tag="PWU81_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL1109444.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            914..1546
                     /locus_tag="PWU81_000715"
     CDS             914..1546
                     /locus_tag="PWU81_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL1109445.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1669..2307
                     /locus_tag="PWU81_000716"
     CDS             1669..2307
                     /locus_tag="PWU81_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL1109446.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(2426..2498)
                     /locus_tag="PWU81_000717"
     tRNA            complement(2426..2498)
                     /locus_tag="PWU81_000717"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2463..2465),aa:Thr,seq:cgt)
     gene            2619..2861
                     /locus_tag="PWU81_000718"
     CDS             2619..2861
                     /locus_tag="PWU81_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109447.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(3000..3629)
                     /gene="upp"
                     /locus_tag="PWU81_000719"
     CDS             complement(3000..3629)
                     /gene="upp"
                     /locus_tag="PWU81_000719"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL1109448.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(3763..4710)
                     /locus_tag="PWU81_000720"
     CDS             complement(3763..4710)
                     /locus_tag="PWU81_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL1109449.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            complement(4863..6107)
                     /locus_tag="PWU81_000721"
     CDS             complement(4863..6107)
                     /locus_tag="PWU81_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL1109450.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            complement(6298..7323)
                     /locus_tag="PWU81_000722"
     CDS             complement(6298..7323)
                     /locus_tag="PWU81_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL1109451.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            complement(7345..7524)
                     /locus_tag="PWU81_000723"
     CDS             complement(7345..7524)
                     /locus_tag="PWU81_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109452.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            complement(7517..8356)
                     /gene="prmC"
                     /locus_tag="PWU81_000724"
     CDS             complement(7517..8356)
                     /gene="prmC"
                     /locus_tag="PWU81_000724"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL1109453.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(8349..9422)
                     /gene="prfA"
                     /locus_tag="PWU81_000725"
     CDS             complement(8349..9422)
                     /gene="prfA"
                     /locus_tag="PWU81_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL1109454.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(9425..10009)
                     /locus_tag="PWU81_000726"
     CDS             complement(9425..10009)
                     /locus_tag="PWU81_000726"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL1109455.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(10115..10584)
                     /locus_tag="PWU81_000727"
                     /pseudo
     CDS             complement(10115..10584)
                     /locus_tag="PWU81_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            10690..11292
                     /locus_tag="PWU81_000728"
     CDS             10690..11292
                     /locus_tag="PWU81_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL1109456.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(11396..11965)
                     /gene="lepB"
                     /locus_tag="PWU81_000729"
     CDS             complement(11396..11965)
                     /gene="lepB"
                     /locus_tag="PWU81_000729"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL1109457.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(12195..12962)
                     /locus_tag="PWU81_000730"
     CDS             complement(12195..12962)
                     /locus_tag="PWU81_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109458.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            complement(12959..13438)
                     /locus_tag="PWU81_000731"
     CDS             complement(12959..13438)
                     /locus_tag="PWU81_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HDL1109459.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(13563..14300)
                     /locus_tag="PWU81_000732"
     CDS             complement(13563..14300)
                     /locus_tag="PWU81_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL1109460.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            14461..15861
                     /locus_tag="PWU81_000733"
     CDS             14461..15861
                     /locus_tag="PWU81_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDL1109461.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            16084..17436
                     /locus_tag="PWU81_000734"
     CDS             16084..17436
                     /locus_tag="PWU81_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HDL1109462.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            17429..18127
                     /locus_tag="PWU81_000735"
     CDS             17429..18127
                     /locus_tag="PWU81_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDL1109463.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(18189..19340)
                     /locus_tag="PWU81_000736"
     CDS             complement(18189..19340)
                     /locus_tag="PWU81_000736"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL1109464.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(19353..19790)
                     /locus_tag="PWU81_000737"
     CDS             complement(19353..19790)
                     /locus_tag="PWU81_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1109465.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(19805..21571)
                     /locus_tag="PWU81_000738"
     CDS             complement(19805..21571)
                     /locus_tag="PWU81_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL1109466.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            21835..22512
                     /locus_tag="PWU81_000739"
     CDS             21835..22512
                     /locus_tag="PWU81_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1109467.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            22515..23642
                     /locus_tag="PWU81_000740"
     CDS             22515..23642
                     /locus_tag="PWU81_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1109468.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(23842..24282)
                     /locus_tag="PWU81_000741"
     CDS             complement(23842..24282)
                     /locus_tag="PWU81_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1109469.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(24293..26326)
                     /locus_tag="PWU81_000742"
     CDS             complement(24293..26326)
                     /locus_tag="PWU81_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL1109470.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(26328..28031)
                     /locus_tag="PWU81_000743"
     CDS             complement(26328..28031)
                     /locus_tag="PWU81_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1109471.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(28125..29075)
                     /gene="manA"
                     /locus_tag="PWU81_000744"
     CDS             complement(28125..29075)
                     /gene="manA"
                     /locus_tag="PWU81_000744"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDL1109472.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(29114..30139)
                     /locus_tag="PWU81_000745"
     CDS             complement(29114..30139)
                     /locus_tag="PWU81_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL1109473.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(30348..31217)
                     /locus_tag="PWU81_000746"
     CDS             complement(30348..31217)
                     /locus_tag="PWU81_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HDL1109474.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(31921..33813)
                     /locus_tag="PWU81_000747"
     CDS             complement(31921..33813)
                     /locus_tag="PWU81_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1109475.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(33810..35537)
                     /locus_tag="PWU81_000748"
     CDS             complement(33810..35537)
                     /locus_tag="PWU81_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1109476.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(35553..36203)
                     /locus_tag="PWU81_000749"
     CDS             complement(35553..36203)
                     /locus_tag="PWU81_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL1109477.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            36407..36703
                     /locus_tag="PWU81_000750"
     CDS             36407..36703
                     /locus_tag="PWU81_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL1109478.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            36895..37515
                     /locus_tag="PWU81_000751"
     CDS             36895..37515
                     /locus_tag="PWU81_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDL1109479.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(37607..38773)
                     /locus_tag="PWU81_000752"
     CDS             complement(37607..38773)
                     /locus_tag="PWU81_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL1109480.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(38785..39138)
                     /locus_tag="PWU81_000753"
     CDS             complement(38785..39138)
                     /locus_tag="PWU81_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL1109481.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            39266..40288
                     /locus_tag="PWU81_000754"
     CDS             39266..40288
                     /locus_tag="PWU81_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDL1109482.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            40307..40468
                     /locus_tag="PWU81_000755"
     CDS             40307..40468
                     /locus_tag="PWU81_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109483.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(40727..41161)
                     /locus_tag="PWU81_000756"
     CDS             complement(40727..41161)
                     /locus_tag="PWU81_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109484.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(41271..43130)
                     /locus_tag="PWU81_000757"
     CDS             complement(41271..43130)
                     /locus_tag="PWU81_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL1109485.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(43248..44408)
                     /locus_tag="PWU81_000758"
     CDS             complement(43248..44408)
                     /locus_tag="PWU81_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDL1109486.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(44715..46139)
                     /gene="araA"
                     /locus_tag="PWU81_000759"
     CDS             complement(44715..46139)
                     /gene="araA"
                     /locus_tag="PWU81_000759"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDL1109487.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(46155..46853)
                     /locus_tag="PWU81_000760"
     CDS             complement(46155..46853)
                     /locus_tag="PWU81_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL1109488.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(46810..48471)
                     /locus_tag="PWU81_000761"
     CDS             complement(46810..48471)
                     /locus_tag="PWU81_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL1109489.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            48689..50089
                     /locus_tag="PWU81_000762"
     CDS             48689..50089
                     /locus_tag="PWU81_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDL1109490.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(50341..51420)
                     /locus_tag="PWU81_000763"
     CDS             complement(50341..51420)
                     /locus_tag="PWU81_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1109491.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(51506..52465)
                     /locus_tag="PWU81_000764"
     CDS             complement(51506..52465)
                     /locus_tag="PWU81_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL1109492.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(52477..53988)
                     /locus_tag="PWU81_000765"
     CDS             complement(52477..53988)
                     /locus_tag="PWU81_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL1109493.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(54010..54573)
                     /locus_tag="PWU81_000766"
     CDS             complement(54010..54573)
                     /locus_tag="PWU81_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDL1109494.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(54579..55442)
                     /locus_tag="PWU81_000767"
     CDS             complement(54579..55442)
                     /locus_tag="PWU81_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1109495.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(55456..56352)
                     /locus_tag="PWU81_000768"
     CDS             complement(55456..56352)
                     /locus_tag="PWU81_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1109496.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(56527..57816)
                     /locus_tag="PWU81_000769"
     CDS             complement(56527..57816)
                     /locus_tag="PWU81_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1109497.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(57788..59305)
                     /locus_tag="PWU81_000770"
     CDS             complement(57788..59305)
                     /locus_tag="PWU81_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL1109498.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            59503..60393
                     /locus_tag="PWU81_000771"
     CDS             59503..60393
                     /locus_tag="PWU81_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDL1109499.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(60466..61290)
                     /gene="nadE"
                     /locus_tag="PWU81_000772"
     CDS             complement(60466..61290)
                     /gene="nadE"
                     /locus_tag="PWU81_000772"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDL1109500.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(61302..62774)
                     /locus_tag="PWU81_000773"
     CDS             complement(61302..62774)
                     /locus_tag="PWU81_000773"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL1109501.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(63411..63773)
                     /locus_tag="PWU81_000774"
     CDS             complement(63411..63773)
                     /locus_tag="PWU81_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL1109502.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            63996..64748
                     /locus_tag="PWU81_000775"
     CDS             63996..64748
                     /locus_tag="PWU81_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL1109503.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(64888..65811)
                     /locus_tag="PWU81_000776"
     CDS             complement(64888..65811)
                     /locus_tag="PWU81_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1109504.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(65826..66098)
                     /locus_tag="PWU81_000777"
     CDS             complement(65826..66098)
                     /locus_tag="PWU81_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109505.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        19192 a        13557 c        11489 g        22032 t
ORIGIN      
        1 tttttcaatc tatatttcca atttttaact tacaaaaatg caacattaat aaataacgat
       61 agtttcataa aatacacttt taattaacat tattagttta tactagctgt ttttgtgtta
      121 attaaaggat aaaatacaat ataaagaaac atctgtttct ttatttagaa taaaaaaata
      181 cagactctgt tcaaataaat gaacctagcc tgcattatga gattgactat actttttaat
      241 tcattgagat gaataactcg tttttataaa tgcccaaaag taatcggtgc cgaagccatt
      301 cctcgaccta attctttttc aaattttcct attgttgata tctccatata aaacggtgat
      361 actggtttag acaattctcc cctgattgct aacggacgta aactacgttc caaattattt
      421 atgactttag gataatactg acctttttct aagcctgtct ttgcgtctat caaaagtata
      481 cgttcctttt ctttgatctc tggatttaaa acgagatcat ataattttag taccatgttt
      541 tctatttctt cacttttaga catcacgatt tcctcccaaa aatatatttc ttcccactac
      601 attctataaa caaagtatac gctacatcta gattttttta aatcattact ggattgttaa
      661 aacctttcaa tacactgtta tggcaacgat ttttatttta aaaacattct aaattaaatc
      721 agtaaaaatt tttattctaa gatttttatt agaattacgc ctaatttgtg tctctcaggg
      781 caattctaag aacgtttcaa gagatagcga cactatcgtt atcgaaaaag gcgaaaacct
      841 atctcatttt ctgctaatgt aagttggttt agcttttgtt aatatgactt tatgaaaatg
      901 gaggtaatta aatatgattt ttcctaaagt taatgaagca agcttgaata aaggatcctt
      961 ttttcatatc tatgaaaacg aagattttga actgtataat atcctttcac aaaaagaggt
     1021 catcgaaaac caaataatct gccatccaat tatttacaaa tctattttca agtatgcatt
     1081 ttttgcttcc aatgattttc aaagaatgga gattaccgac tgtatattcg aaaactgtga
     1141 tttttcaggc tgtatattga caaaaagcat gttgcatcgc gtgaaatttg tcaattgtaa
     1201 attaacaggt actaaattga tggaaagtta tattgggaac acgttattcg agaattgtaa
     1261 aatggactat gtaaatttat ctggttcaaa cataaaagaa agtacctttg agcactctgt
     1321 cttatcaagc gcagattttg tagactgttc cctcactaaa ataatgtttt ctactaatga
     1381 tttagaaaat gctagctttt ttgatactga tttaaaaaat atcgatttaa gtagcaactc
     1441 ctttgaaatg attgaagtac gctcggaaca tctaaaaaat tgtacgctta atcagttgca
     1501 agcacttggt tttgctagaa aatttttaca aataaaagta gtataatcaa aatgaatata
     1561 taagataaat ttttagcaat acaaatatcc ataaaaaaac attgcctatt ttaactcttc
     1621 atgtatactg actctatctt caaaaactga atagtaggag ttattatcat gaataacatt
     1681 ttaggaaaaa aaatagaact taaaaaaggg atattgggca ctattgtcgt tgtactcggt
     1741 ttgttagggt tgctatttgc gccatccatt attagcattt tttctttaac tatcactgga
     1801 tttatagcat ttacacttgt ttttggtaaa aaagatacga aaaaaatgtt ctcgaaacct
     1861 gtcgctcctg tcaaaaatat agccaaatat ttttttatca atgtagtaat cagtgccgca
     1921 gtctcggtat ttttgcaaca aattttgaaa tggggtttaa caggcaatcc aataaatgag
     1981 gctttcagcc cgttattatt tattattttg cctatcatga tacttggaga agaattgttc
     2041 tcagtgtatt ttctcgctat tttttctagt aagttcagca ttcctgtcgc tagtttcttg
     2101 tcagcaatca tctttggatt tatccattac agtacttatg ataatggaaa tattttacac
     2161 actgtagcac acatcctttt gatacaaggg gtggcaagat tgttattcaa ccaagctgcc
     2221 attaaaagca actcgatcat tacaagttgg gcagttcatg tgatatttga tttcacagct
     2281 atattgctag tcgttttatt ctcctaaatt tttatagaaa taatacatgc tctattgcta
     2341 agaaagcata ctttatttcc tttaaacaaa aagacagatg tatcaatgga ttaaaaatcc
     2401 ttgatatatc tgtcttctct tttaatgcca gctataggga tcgaacctac gacctacgcg
     2461 ttacgagtgc gttgctctac caactgagct aagctggcct tatccaaaag aaattatata
     2521 ctctatttgg ctgattgtaa agagtccgat ggaaaattag tttaatttca tgatattttt
     2581 cataaaacgt ggtataattg atgtgaaagg caggttttat gaaaatggca acttttggaa
     2641 aatttgatac aactatcgta ccatcggaaa ttggtgaaaa attcaagatc aatactcacg
     2701 taatgttcac aattcacggc aagtcgcaaa ctggaacgat ttcaaagcaa ttgaaaaatt
     2761 ccgctgttgt tgagattgat gaaactgcag aaaatgcgaa cttgatgacg caaagtaagg
     2821 gtgtagtaat cgttaactac aaagaactaa aaaagatttg aatttgacca tctctgcatt
     2881 tttgggatgg tttttttata tgcaaacgaa agctgtgaaa aagctgttct gcatcaaacg
     2941 ttaaaaatga ccaaaaaagg atgtgacaaa agttgtgtca cacccttttt cgtatttact
     3001 tattttgtac caaacaaacg gtcacccgca tcccctagtc caggaacgat ataaccatgt
     3061 tcattcaatt tttcatctag agaagctgta tagatgtcaa tatcaggatg cgcttcttgc
     3121 aaagctttta ctccctcagg tgctgctacc aaacaaacaa atttaatgtt cgacgcgcca
     3181 cgttctttca atgcatcgat tgccatgatt gctgatccgc ctgttgccaa cattggatct
     3241 actacaaaca actgacgtgc atcaatgtct tctggaagtt tgacgaaata ttcatgtggt
     3301 tccaatgttt cttcatctcg ataaagaccg atatgtccca ctttagcagc tggaatcaat
     3361 tccaagatac cgtcaaccat tccaatacct gcgcgcaaga ttggaataat cgctactttt
     3421 ttaccagaca acgttttttg tgttgtttca gtgatcggtg tttcgatcac gatgtcttcc
     3481 aatggcatat cccgtgatac ttcatatgcc atcaacatag cgatttcatc gactacttca
     3541 cgaaaaacct ttgttccgca gtttttgtcc cgaataatag ttagtttgtg ttgaatcaat
     3601 ggatgatcga tcacttgaaa tttgcccatt ttttaagctc tcctttgttc tgtcgtttct
     3661 ttttaattgt aaaacaaaat caagtgattg actagtcaaa ctcgccattt ttcctaagaa
     3721 aatccgtttt tgaaaagaag ttttcctgcg gacaagcaaa gcttattctt ttttcttgat
     3781 ccaagaagta gataatgcca ttgctgcacg cgttgccttt gtctgatcca tagcatttac
     3841 catgacaaaa tcatggatga ttccttggaa gcgtacttgt gtaacaggaa cccctgcatc
     3901 acgaagtttg cgcgcataag cctcaccttc atctcttaat acatccgctt ctcccgttaa
     3961 aatcattgct gaaggaagat catttagttc atttatattt gctctcaaag gcgaagctgt
     4021 gatttcagca cgctcttctt cattcgtcgt atattggtcc caaaaccaca tcattccttc
     4081 tcttgtcaaa aaatagtttt ctccgaattc atgataggaa tctgtatcaa aatcagcgtt
     4141 tgtgactgga taatacagca attgcttgga tattgggagg ccctttcgtt cttttgttaa
     4201 gatcgtcatg actgtagcca tgttaccacc tactgaatct cctgcaacga ttagattctc
     4261 gagatccaga ttttttgact tggctaattt attcagctgt tgcaacacag cataattttg
     4321 ttcaattgct gttggatatt ttgcttctgg tgatcgacta tattctggaa agacaacgac
     4381 ggaattcgta cgaaccgcaa gttctctgat caatttatca tgggtttgag cactgccaaa
     4441 aacccagcct gctccatgaa tataaaaaat aactggtaat ttatccatat tcccttctgg
     4501 tcgtacgaga cgaatattga gttttcccca ttcgccagta tccaccatca agtcttcaat
     4561 atcgacggaa tatttgttca caggtgattc ttgtacttct tctagtaaaa ctcttccttc
     4621 ttccggtgct agttcataga tacgcggatg aggcgcattt gcatcactga atgctactgc
     4681 ttccttttct aacgagatac gatcagtcat gacttttcct ccctgagatt tagtgtgaag
     4741 actaaaatta cacctcatat actagagagc atcaccgata ttgtcaaaga atccgttttc
     4801 ccaaaaaata aacagaaaag cccaacattg aacttttctg tttactaatg gaatttaaat
     4861 ttttattttt tatgcaacgg aaatttttca gttaattcgc gtacacctgt tttgacttcg
     4921 tctagttgag cgttatcatc ttttgcttgt aatgctttga caactagttc agccactttt
     4981 acagcatctt cttctttgaa cccgcgagta gtgattgctg gcgtgccgat tcgaataccg
     5041 cttgttttga atggacttag tgtttcaaaa ggaatcgagt ttttatttac agtgatattc
     5101 acactgtcca aaatgctctc tgcttctttt ccatttatac ctaactcgcg aacatcgatc
     5161 aacatcagat gattatctgt cgcccctgaa atgactctgg ttccaattgc ttgattgaaa
     5221 actttgacca ttgcttttgc attggcgatg atttgttcac tatattcttt aaatgcagga
     5281 tctaatgctt ctttgaatgc tactgcttta cccgcaatca catgctctag agggccgcct
     5341 tgaataccag gaaataccgc actatttatt ttttttgcta atgcttcatc gtttgtcaaa
     5401 atcatgccgc ctcgagggcc acgcaatgtt ttatgagtgg tagtcgttgt gatatctgca
     5461 taaggaactg gattaggatg aagtcctgct gctactaatc ctgcgatatg tgccatatct
     5521 accatcagtt ttgcgcctac ttcatctgcg atttctctga atttcgcaaa gtcgatcgtt
     5581 cgtccgtaag cactggcacc tgctacgatc aattttggtt gatgttttct agctaaaata
     5641 cgtaccacat tataatcaat aacttctgtt gtcggatcaa caccgtaagc aacaaagtga
     5701 taggttttgc cactgaagtt gactggtgat ccatgtgtta gatgtccgcc tgcggaaaga
     5761 tccattccta agatcgtgtc ccctggttct accaaagcga gataagcagc cgtgttggct
     5821 tgagagccgg agtgaggctg gacgttcgca aatttcgctc cgaataattc ttttgcacgg
     5881 tcaatcgcta aattttcaac gatatcgaca aattcacacc caccataata gcgatgccct
     5941 ggataacctt ctgcgtattt atttgtcaag atacttcctt gtgcagccat cactgcttcg
     6001 gagacaaagt tttcagaggc aatcagctcc aaattatgtt cttgtctttc ttcttctttg
     6061 gcgatcgcag cccaaagatc agggtcaaac gttttgtaat ctaccacaaa caacagctcc
     6121 tttttatttt cctacaattt tattttccta caattttatt tttcccatca aaaacaaatt
     6181 gccaaatatt cgtatctctt ctattacgat tagttacgct tagtttttcg ttcactgata
     6241 gaagatttac ttgatagcat tgtatcatac taaaagaatg cgacaataac caattgatca
     6301 tttttcaaaa taattttgtc ctgctgcttt tttcaagcga ttcatgtaag cattccccaa
     6361 tccagtttct ggatacactt gtacaaaaat caaatctaat ccaagtgttg gttcatctaa
     6421 tcctcttaga cctgcaaaca atcctttagc cgccgcttct actgagttgt cgttatacat
     6481 ataaacagct gcagtatctg tccgcacttg atccgcaatt tcggggcttg ctatgacacc
     6541 tgcacggatc ttcttatttt ttgcccattg aactgcagtt gaccaatcgc catctctgac
     6601 catcagaaca cgtgtatcgg gtgagtagtg tttgtatttc atcccagggg cttttggtgt
     6661 ttcattttct tttacaagat gctgatcaat agcaacagga gattcgatga ctgcttcgat
     6721 ttgttctttc gttactgcac ctggtcgtaa aatcataggc atggctgttg gatcacttaa
     6781 atcaagaaca gtggattcta caccaattcg cgtagctcca tcatcaataa tcccggtaat
     6841 ttttccttgt agatcatgat atacatgatc tgcagtcgtt ggacttggtt ttcccgacgt
     6901 atttgcactt ggaccgacaa gcggaacacc ggaaagttcg attacttcta gtgttttttt
     6961 gttatccggt atacgaaatg cagctgtgga taatcctccc gtcacaactg agggcaaagt
     7021 gtcttttttg atcttaaata tcaaagttaa tggtccaggc cagaagttct tcacaatctt
     7081 ctctgctgcc ggatgaaagt tgtccacata ctcttttact tgttcaaaag aggcgacatg
     7141 gacgattaat ggattatctt gcggtctccc ttttacagaa aacactttct tgacagcgtt
     7201 tggtaaaaga gcgttggcgc ccaatccgta tactgtttcg gtaggaaaag caacaagttc
     7261 tccttctttc agtgcttcgg ctgcctcttg caattctgtt tcatcatatc gtttcgtttc
     7321 cacagtttat ccacaacccc tttttcatac attcttattt tgtctgagtt tatcctcaag
     7381 ttttccactg tttgtggata atctatttgt caatagtgga taaacttgtt gataaaccaa
     7441 aaagtcgttt acatccttgt caaataagtg tttttttata ataaaaaaac aaataaaaaa
     7501 tttttcatcc acaacttcaa ccataaactt ggatcattcg ttcattgcca aaaagatcct
     7561 tttttatcgc cactttttta tctggaaatg cttgctgaaa tatttttttt actgcttcac
     7621 cttggcgaaa tccgatttct aaaaaaatct ttccatttga ttttagtacg cttggtgctt
     7681 caactgctat tttttcataa atagctaatc catcgttctc agcaaaaagg gccattttag
     7741 gttcgaatga ccggacactt tcatccatca gttcccattc attccgactg atgtacggcg
     7801 gattagaaat aatcacatca aattgctgat ccattactgg tcctaatgta tctccatgat
     7861 aaaaactgat tttcgttcct tctttctgag cattttcttt cgctacttct aatgcttctt
     7921 ctgaaagatc cacagcagat atatgccaat tttttcttgc tgctttcaaa ctaatagcaa
     7981 ttgctccagt ccctgtccca atatctatca cttctaacgg tacaccaggc gtctcgtcta
     8041 aacaccattc gactaactcc tctgtttctg gtcgagggat cagtgttgct tcagttacct
     8101 ttaaccgatg atcataaaaa tctgcatatc ctagcaaata ctgaggtgga tgatctgcaa
     8161 gcagaagtcg cagatcggtt tcgatcaacc gctgttcttc ttctgaaatc tcttcgttca
     8221 tatgaaggag ccaatcaagt tttttccatt gttttcgttc caaaaataaa aattggatac
     8281 tatatgcttc tttcccatga tcctctaaaa aagaagaagc ccgtgacagg acttctcgat
     8341 aggtcatttt acccattttg catagcttcc agttttgatg tttgatcata catgatcaat
     8401 gcatcaacga tctcatctaa tttccctgct aaaatctgat ctaatttttg gatagttaat
     8461 ccgatacgat gatcagtcac acgattttgc gggaaattat acgtccggat ccgttctgaa
     8521 cgatccccag ttccgactgc tgattttcga ttggcatcat actcgctttg tgcttcttgc
     8581 tggatcttat cgtacacacg agcacgcaag attttcattg ctttttcacg gttcttgatt
     8641 tgagaacgtt catcttgcat agcaacaacg atccctgttg gtaagtgagt caaacgtaca
     8701 gcagatgctg ttttattgac gtgctgtccg ccagcacctg aagcatgata gatatctgta
     8761 cggatatctt tgtcttcaat atcgatctct acttcttcag cttctggcat cacgacaact
     8821 gttgcggtgg aagtatggat acgcccttgc gattcagttg aaggaacacg ctggacgcgg
     8881 tgcgcaccac tttcatattt taatttcgaa tatacgttgt ctccggtgat catcatgatc
     8941 acttctttat atccaccgat tccggtaacg ttagcttcca tcacttctgt tttccagcct
     9001 tgtgcttcag catacttttg gtacatattg aaaagatcgc cagcgaataa tgctgcttcg
     9061 tctccgcctg ctgctccacg gatttccatg atgatatttt tatcgtcatt tggatctttt
     9121 ggtaaaagca agatcttgat tttttcttct aagacttctt tttctttttt taattcagat
     9181 aattcttctt ttgctagttc agccatttca tcatccagtt tttcaccaag cagttcttct
     9241 gtatcggtga ttccttgtac tacttcttta taacgacgat aaacttcgac tgtttcgcgc
     9301 gtgtttgctt cttcttttga aagctgcata aaacgctttg tatcagaaat cacttctggg
     9361 tcacttaaaa gttctccaag ctcttcataa cggtcttcaa ttgcttgtaa ttgatcgtac
     9421 atgcttattc tccttctgta atcattgatg ggtgaaagta atgttttcga caaacggggt
     9481 aataagcttc atttccgcca atttgcactt gatcgccttc ataaacaggg tttccatcaa
     9541 tataatgtaa attcatgatt gcttttttgt ggcagaacca acagattgtt ttcatttctt
     9601 cgattttgtc tgcataaagc aataaatatt ttgatccttc aaataactcg tttcgaaaat
     9661 catttttcaa accaaatgcc ataaccggaa tatccaattc gtccacaatt ctcgtaaaag
     9721 caatgacatg tttctttttt aagaattgcg cttcatcgat cagcacacaa tagggtttgc
     9781 tttccatttt ctgaattacg tcaaatacgt ctgtttcttc aaatatcggc atcgcattac
     9841 gtttcagtcc gattcggctt gaaacaacgc ctaaaccatc tcttgtatca ataccgcttg
     9901 tcattaagac aactggctta ttttgttctt cataattatg tgcgactttt aggatctcga
     9961 tcgtcttgcc gctattcatt gcgccatact taaaaaatag ttgtgccatg ctctttttca
    10021 ccttccagtt tgttcctatg gtattatact tgattttcag cattttttac agtgagaatt
    10081 ttcatcaagc acagattagt attaaatcta ttgctcactt ttgttccaat tttttgacaa
    10141 gaactaaaca tgggttccat tcatcccata aatttggaaa aacttccaac cttgaaaatc
    10201 ctaatgattc gtagaaacaa atagtttgat catatataga gtagtgcccc tcgtctacag
    10261 tcttcacttg caaatatttg taatgttttg ctgcgtaacc ttctgcttct ttaaaaagtt
    10321 ttgttcctaa tccttggtga tgatagtctt tttttattcc catacaatag aatagatttc
    10381 ggcagtatct tcacttgtct gtgaaagagt gataaagcct aaatattctt cttttctttt
    10441 ttcacaccat aaaggtagtt tacttgattc ctcgatatat ttttccgttg attcaggaag
    10501 cccaaaccac tcaggcaagt ctagtaatat ttctcggaca cactttcctt tttcctctct
    10561 atcttttaca aacgatatcg gcatattctt tctagattct ccctttttga attgagtata
    10621 gcatgctttc tttcttttca cgtgctataa taagttaaaa tttcaaagtt ttacgtaagg
    10681 agctatttga tgacaaaaaa tatttttgat tccatcgcta accattatga ttcccctgac
    10741 cgtcaagcat tagccaagat tattcgcgaa gaactaacta cttatctacc gcgcgacagc
    10801 caccaaaaag tcctgctgga ttacggaggc ggaactgggc ttgttagttt accgcttgcg
    10861 gaaagattca aagaactgat cattgccgat gcttcagaaa ctatgctgaa aatggcagaa
    10921 gaaaagatcc aagcagcaga tctgaaaaat gtccgaacga tccacgctga tgcttctgtc
    10981 gaatttcctg ctgtccaagc taatctcatc cttctatcac ttgtactttt acacattcct
    11041 gatacggaga atattcttac aaaactttat gaaattttag ctcctggcgg tcaattgatc
    11101 atcgtagact ttgacaagaa tgaacaaatt agtcatccga aagtccataa tggctttagc
    11161 caagaagaat tgaataatcg attggaaaaa acgggctttg tctctgctac tagtcatacc
    11221 ttccatcgtg gggaaaagct atttatgaac aaaaacgctt cgcttttctt atccatcagc
    11281 caaaaagctt aaaatgagat tgcaaaaaaa gtgagcaact ccaagaaata agaaggaatt
    11341 ttcggaaaaa gaagctgtga caaaaaacat gtcacagctt ctttttattt tttcatcaca
    11401 tgattttaat ttctgaaaga ggccaataac ggaaaacaac ttttccttgg atcatatctt
    11461 tgtcgactaa cccaaacatt cggctgtctt ttgaaatcaa gcggttatcg cctagaacaa
    11521 gatactttcc ttttggcaca gtatattcaa agtcactcgt aaaggtggat gaactttctg
    11581 cttgtgcttg gaatcctgag ctataagcat attctccttg taacttatct tctgaaaatt
    11641 cttttttgaa ctcatccaga taaggttcat catacttttt gccattaatg gtcaactggt
    11701 cattttccat cttgactgta tctccaggca taccgatgat ccgtttaaca atcatccgtt
    11761 cttcatcccc cggttccttt gtagtaataa tatccattcg ttcatagttt gagatcttag
    11821 atgaaatcaa tcgttgtcca tcacttaatg tagggtccat cgaatgcccg tttacagtaa
    11881 ctggtgtaaa cacgtacact ctagctaata aaatagccgc taatacaagg atcaaaatcc
    11941 cccaattttt caaaaattct ttcacagctt acctcctact tgtattttcc tcgttattta
    12001 ttacgttttt tcacaatact tcgttgtact ttctttattc tacaatgttt ataaatgaaa
    12061 ggaaagaata agtgatgtgt tcgtcttatc tttctcgtta tataaaaaag gaacgtgaca
    12121 aaagacgtgt cacatccccc ttattccgaa taaacgtttt tatcgcaacc actgatacac
    12181 ggcgtttcat cacattatga atgtcttctt ctttggctac gactataaac cactggttct
    12241 tcctgcgact gtgccttttt tgcaatcaat ttcagacgat aaggctcatg atagcgtaac
    12301 ccgtctttga caaaatcacc gccgacgatg aacaatccga caggttccaa tgtgtcttct
    12361 gttgagtcaa gtcgaatggt ccctactttt tccatgtccg agtagcttcg tccaatattt
    12421 agaacatact ctttctctcc tgttttcttg acatagtaat aaggatacaa tgtatttcta
    12481 tcgaccagaa atccatccat gcgaccgagt tcatttgttt tgatctcacc atttccaatc
    12541 aaataccgca tattctcggc taatttcaat tgataatctt cgtcataaga aaccatgacc
    12601 tcttgtgtat tcactttatc gatcgaattt ccattatcta tcgattcttc tgtctggatg
    12661 ttgaacgctt catcaggtaa atacttcaca aaaagctgag tgacatcggc tttcgtgttt
    12721 ttatctaaaa agtaataaaa tacatttaaa acagtaaaat aaacaagcac tagaaatgca
    12781 agtccatata agaaaaattg aagatagttt tggttttgcc aaagtccata aacaattctc
    12841 aataaataaa gcgtgggaat cacacttaaa atcgtatatc cacggttaag atatttagga
    12901 ctgatgtcta aatagccaag gaatttatgc accccgttga taaggtctag taaaaatgtc
    12961 attgagaatc acctgcttgc tggttatacg tttgggtatt tcctgaatct tggttttgct
    13021 gtgctgtacg tgctgcatca tcattttgcg tgttatcatc tacagtcgta ctgcttggta
    13081 tcgtttcgga gctagcactt gtatccggac ttgtacttgg attggttgaa tccgatggag
    13141 tgccatctgg taccgtatta cttgtttcgc ttgagctgtc agttgtatat gtctcatcat
    13201 acgacgaact agttgttgta tcgtcccatg tacttgtcgt agtggtttca ttcaaacttt
    13261 ccgttgttgt tggttcagat gtcgagactg aagtagttgg tgttgtttct tctacagtac
    13321 tagaacttga acttggtgca cggtagctgg aactattcac aatacctgta gtagtcgcga
    13381 caatcgtcga gattgttaat acagcgattg gcttcaaaat aggtgctact tttcgcatct
    13441 tatctcttcc ttcattgaaa tttattcttg tttccttact ttactacgga caaattatac
    13501 agtctacctt atttctacca taaattcttt atttttttct tacgtttttc cgaaagattt
    13561 ctttaatttt cctcaaaaac tacggttgat gtttttaagt aagcttttgc ttcccgattg
    13621 atttctttca gatcgcatcc ttgctgcttg gcagcgaaca cgcaaccaca ataacattgg
    13681 cgataaatat catactcttt gcacatttgc aaagagcgtt catagccttt gtttttcttg
    13741 aaatcacttg gcaagtattg tgtcccgtaa atcttttgga tatccattcc gatctgattg
    13801 atcaaagctg catttttctt tggcgagatg gtcagggcac ttccaaagta atcgtatcct
    13861 agttcttgcg ctttttgcgc aacaagatct aatcgcatat tgaagcatgc actgcagcga
    13921 gcaccacctt ctttttcgtt ttcaagatgg tttgttaaaa ccattttggt gaattcattt
    13981 ggacggtatt catcaacgat tagcgcaaca tgatggtcag ttttctcatt aaattggtct
    14041 acaaatcgtt tttgttcatg taatcttcgc aaatactcac ttttaggatg aatattcgaa
    14101 tttgaaaaaa ataacgtgat ctctgcatat tgagacatga attccaatgt gtgcgtactg
    14161 caaggagcac agcatgtatg tattaatatc ttaggcttta cttcttcttt ctcccattgc
    14221 atgatcattt ttttcagaac tgtatcataa tttatttttt gatttttcat tttttctacg
    14281 atttgttgag cattaatcaa tttgttattc ctcctccttc ttttttctag gattttcctt
    14341 attataagcc atttctattt taatcgaaag ctgtattgtt atctgtttga taatttcttt
    14401 gagtattcct aattcccctt gtatactggt agaaacgact attgtcagga ggatagtact
    14461 gtgcatgttg atttattctt tcgaaggctg cagcggcgag gacagaagtt tgctgccaat
    14521 catttctcat cgatccaaat catcgttttt tattatatat tgatgacggt cttatcactc
    14581 gttctatttt atatgccgat ttttcgcgaa ccagatagcc atgtttcttt tgtggacatg
    14641 ctgttcatgg caatcagtac agtgagtgtg actggactat cgacttttga tatcaattct
    14701 gtgttcaatg ataacggtat tatactgctg gaaatattat ttcaagttgg tggtttgggg
    14761 atcatgatga tttctactgc ttttgtcatt ttttctaaac gacggatcac actgaaacag
    14821 cgacagctga tcatgacgga tatgaatcaa ccgcgtttgt ctggtatcgt ccgactgatt
    14881 cggatcactt ttgctatttt aatctggttt caattacttt ttgggacatt cttttctatt
    14941 tatttttatt acagaggcta ttttgatcgt tggcgtgatg ccgtttttta cggtttttat
    15001 caggcgatat cggctgtcac gaactctggg tttgacgtca ctggggattc catcaagccc
    15061 tttgcacatg actatttttt cttgatcctg atcatgtttt tgatttttgt cggaggaatt
    15121 ggttttcctg tattgatgga gtgccgggaa tggctattat acaaacgttc caatgcgaag
    15181 cttccgtttc gtttttcctt gtttaccaaa ttagctgtgt tagcttttgt tatcttgttt
    15241 gtttcgggaa ctgtcttgat ttatctattg gaaaaggacc atttattcca agattccaat
    15301 atgagtgtca aatggatcaa ttcgatgttt tattcgatta cgactcgtaa tgctggtctg
    15361 cagattcacg atttgggtga ttttcaaatc acaacactga tcattttttc tttactgatg
    15421 tttatcggat gcagtcctag ttctgtaggc ggcgggattc gaacaactac tgtagcaatc
    15481 atcggtttgt atctatattc cttcctcaaa agtgaagata acatcaatat ttttggacgg
    15541 agaatcgatc aagatgatgt gcgaaaatca gtcgttgtct ttatgctttc tcttggtatg
    15601 tgtttctttt gtatcgtttt tttatctgct acagaagaac aaacgctgat ctcgatcatc
    15661 atcgaagtaa cttctgcgtt tggaacaact ggtttatctt tagggattac cggagacttg
    15721 tctgttgtgg gcaaaatcac gattgctaca ctgatgttta ttgggcggat cggaatgcta
    15781 tatacattga tgttattcgt gccaaaagaa acacgagatc taggctatga atatccaagc
    15841 gagaaaatta ttatcggtta aaatgggaat ataggtaaga gggaataata aaatcacagc
    15901 agcttgaaac aatcattttg tttcaagctg tttttttgtt aaatcatgcg attttgtccg
    15961 atttcatgca ctttcagccg taaccttttc ttaagaataa gaaaagcaat ttaacctttt
    16021 tttcaacata tggtatgatt agttgaattt attcgtcagc aaatcgtttt tggaggttac
    16081 ataatgggaa tacgcagtca ttttgcgatt gcagttggaa aaacgtcgca atgggcatta
    16141 aaaacttttt ttaaaggcgg atcaagttta ccaggtaaac tagcgctgaa agtcgatcca
    16201 catatactag atagtttggc aaaagactac caagtcgtcg ttgtcaccgg aacaaacggt
    16261 aaaacattga ctaccgcttt aacagtcaat attttgcgcc agcaattcga tgaagttcta
    16321 accaacccta ctggtgcgaa tatggtacag ggaattgtat cgactttctt gcaggcaaaa
    16381 ccaaaaaaag gacagaaaaa gtttgctgtc ctagaaatag atgaagcaag tttgagcaaa
    16441 gtcacagaat atatgaaacc agaactgttc ttatttacaa atattttccg tgatcaaatg
    16501 gatcggtatg gagaaatcta tacgacctat aaaatgattg tagacggtgc cgcaaagtca
    16561 ccaaatgcaa cgattatcag taacggtgat tcaccaatct tcaattctat cgaaacagtc
    16621 aatcctagaa aatattatgg ctttgaccat gaagaagatc gtgaacaaat ggctcattac
    16681 aatacagacg gtgtcttatg tccaaattgt catcacatct tgcattataa aatgatcact
    16741 tatgcaaact tagggaaata ttactgtcca aattgtgact tcaaacgtcc tgaattggat
    16801 tatcgattaa ctgatatggt acggatggac aatgtgtcag cagatttcgt gatcgatggt
    16861 catgaatacg gaatcgaagt tggcggtatg tataatgtct acaatgcttt agcagctact
    16921 gcagtggcag agtacttcaa tgtcgatcct gataaaatca aacaaggttt aggatatgac
    16981 gagaaagtat tcggccgaca agaagtcatc aatatcaatg ggaaaaaatg tacattagta
    17041 cttgtcaaaa atcctgtagg tatcaatcaa gtaatcgata tgattggtct agctccttat
    17101 ccattttctt tagtggcttt attaaatgcc aattacgcag acggaatcga tgtcagctgg
    17161 atttgggatg gtaattttga aaactttgcc gatatggata tcccagctgt tatttctgga
    17221 ggagaccgtc atacagatat gactttacgt ctaaaagttg ccgggattcc agaaaataaa
    17281 ttgacagaaa caaaagaatt accagaagta atcgaaaaaa tcaaagaatt gccaactgag
    17341 catgtctata ttttagctac ttacactgct gtattgcagc ttcgtaaaga actagctgct
    17401 caaggattta tcaaaggagg aatgaaccgt ggctaattat gaactcaata tcgcccacct
    17461 ttatggaaat ttgatgaaca catatggtga taacggaaac cttttgatgt tgaaatatat
    17521 agcagaaaaa atgggggttt cttgccatac ggagatcgtt agtatacatg aaccttttga
    17581 tgctgataaa tatgacttgg ttttctttgg cggtggtcaa gactttgagc aattaatcat
    17641 ttcaaaagac atccagaaaa agaaagattc tttgatcaac tatatcgaaa atgatggcgt
    17701 catgttagcc atttgtggtg gctaccaatt gctaggtcac tactacatgg gagctaatgg
    17761 agaaaagatc caaggaatca gtgcactgga tcactacacg ttaagtcagg aaaataaccg
    17821 gtttatcggt gatatcgtta tccataacga agaattcaat gaaacttatt atggatttga
    17881 aaaccacaat ggtcggactt tcttaggtga aggtgaacga cctttgggga aagtggtaca
    17941 aggtcaagga aataatggtg aagaccaatc tgaaggtgtc atttaccgaa atgtcttcgg
    18001 ttcttatttc catggaccta tccttgcaag aaatgagaac ctggcagctc gtttgatccg
    18061 cctagcttta gaacaacgtt acggtaaaga attggatctg ccctatcttg aaggacaaac
    18121 agcctagtta ttcgaaaaag gggaatgtga caaaaatttc gtcacattcc cctttttcgt
    18181 atcctatttt aagcgacttg cttggcataa cggttcacgt tttgcttgat attcccgatt
    18241 gttttcggat cattgattcc tgtgacttct tgaatcaatt gttccaagtt ttcatggatc
    18301 ttcatttcat gcaattgacg gctttgttca tcttcaggat catagtaatt aaaaataatc
    18361 cccattgttg ccgtcaaatg tgggcaagtc aagttatgtt cttgtagttc tctcactgga
    18421 cgtgtaaatc tttcttgata acctaactta cgtaatggtg tccgagcgac acgtgtgatc
    18481 gcatcagaga tatttttgtt ttggaagcga tgaatgattt tttcaatata cgcatgatgc
    18541 tgctctgcat caaaccccca tttggcaatc agcaaacttc ctgtttcctg caaaactgat
    18601 tttaattgtg ccacaactaa cgcatcttgc attgcttcat caatggtctg gtaacctaac
    18661 aaggcacctg tataagcgac agtcgcatga ccagtattga cactaaatag tttgcgttcg
    18721 atatatggtt ctagatcatc tacatataaa acccctttta attggatttt tttatttttt
    18781 ctttggctat cgtcaatgac ccattctttg aaaggttcca cttggacgaa taaaggatct
    18841 tcatggtgtt gtaatgggac gattcgatcg actgctgcat ttggaaagcc gatatatttg
    18901 tcagtaaatg atgtttccgg aagatattta tacacttctt tttctagaaa tgctgaaccg
    18961 ccaatcatat tttcacaggc aatgatatct aaagatgttg tgcttccttc cttttctctt
    19021 tcttggatac cttttgcaat caactcagca ataaaaggta agatattcgg gccaatggcg
    19081 gtagtaacta aatctgcttc agcgatttct tggattactt tttctggatt tgtttgattg
    19141 ttgattcctt tgacattttt tacagtgatt ttttcttttg attcatctgc taattcgatt
    19201 tcgtattctt ttctttcatt taaagcatca atgatcgtgt gattcacatc tacgaaagta
    19261 atctcaaatc cattagctgc caaaacctct ccaataaatc ctcgaccgat atttcctgca
    19321 ccaaaatgta ctgctctcat ttttctctct ccttaagcaa ttgctagatt ttccgttact
    19381 tcttctttgc tgagcgcgtc tgctagctgg acaacattgt ccatatcact gcaataaagt
    19441 gcgatttgct gtacaagttg caaatgctca tctccgactc ctgcaatacc gaacaaaaca
    19501 gtcgcaatct tttcttcttc ttcggtacca aaatctacac catctggtac ttgaacgaca
    19561 cagatgccgg attttttcac atattttttt gcttcatctg ttccatgagg aatcgcgata
    19621 aaattcccca tataaacaga taacattttg tctctatcta ccatggcatc gatatattcc
    19681 tcatcaacgc accctgcttc tacaagcatc tgaccgcaat agcggatcgc ttcttctttg
    19741 gttttaaaag atcgattgag ctcgatcatg tcgaatggca aactttgcat gctgttttcc
    19801 tcccttaagc tgtctgtttc aaatattgtg caatcgattc gtatttttgc ggattgacca
    19861 tgctgttgac tgataaatga atggattgag gtgcagccat ctgtactttt tccgctaaac
    19921 tttctttggc tacgatcagt gaatcaggtt catcttttaa atcttggata ttgacttttc
    19981 ttaacgggat agttaaatgg ttcttgtcta atatatcttt gagcacggtc atccccattg
    20041 ttgctgatcc ggaacgatca tcgtggacaa gtagaatctc tttgatttgt ttgatgtttg
    20101 ctgaaagaaa ctcttctgct ggctcattct caatttcaga aactgatgtt ttctgcttgc
    20161 tgcttaattc ttgaatgatt tcatcatatc gaggtgaatt caaaaagttt tctactgaga
    20221 caaaacgcgc gctcggtgct ttttgttttg ctctttcttg tagttcgacc tgagtaacga
    20281 tcaatgtatt cgagtcatct gtcagttggc tgattgcttg attggtcacc gtttgaggca
    20341 agtctgcttc ctgtactttt tttctcaaga tcgaagcgcc catcgcactt gatcccattc
    20401 ctgcatcaca agcaaaaatg attttttgta cgccagagaa tgtagctcct tgaggaatac
    20461 cttgggcacc tttggaagct tgcttagctg cctttgtctc ttgaacagat ttttcgaagt
    20521 cttcatttgt atctttcgca tccgctttca aaattactgc agaaatacca aacgaaacag
    20581 ctgcggcaac aatcacgccc aaaataacag gaaggtaaga attgaacggt gtcatcgcta
    20641 gaatcgcaat gatcgaccct ggagaagctg gcgctcttag tccagcatcc atcaattgga
    20701 aaatgaaact tcctgacatt cctccgccta taactgataa gaataataac ggtttcatca
    20761 taacgtaagg aaaatagatt tcatggatcc cgcctaaaaa gtgaatgatc atggctcctg
    20821 gtgctgaaga ttttgctgct cctttgccaa acaaagtaaa agcaagtaat acgccaagtc
    20881 ctggaccagg atttgcttca agcaagaata gaatagattt tccagcctct gctacttgtt
    20941 cagttcccaa tggtgtcaaa atcccatgat tgattgcatt attcaaaaat aagatttttg
    21001 ctggttcaat aaaaatactt gtcaatggaa tcaaatgggc agataaaatg atctctacac
    21061 cttttgccat tgcttgagtc aatgcatcga caactggacc aatcgcagaa aaacctaata
    21121 acgctaatcc aaaaccgata agacctgctg aaaaattatt gactagcatt tcaaaaccgg
    21181 atttgatccg tttttggaat agctgatcaa atttcttgat ggcatatcca ccaagaggtc
    21241 ccatgatcat cgcgcctagc atcattggaa tatctgttcc gacaatcaca cccatcgttg
    21301 caattgcccc aacaactgat ccgcggtcac cagcaattac ttttccgcca gtataaccga
    21361 tcaataaagg aatcagataa gttagcattg ggcttaccat ggtagcgaaa gcctcatttg
    21421 gtaaataacc atctgggata aatagagccg ttaatacacc ccaggcaatc agtgctccaa
    21481 tatttggcat gaccatgctt gataaagtac tgcctaattt ttgtaccctc gcttttagcg
    21541 aaatgttgtt tttgggaatc gtccgttcca ttttgtctct ccctctctcc ttttgatact
    21601 ctaattgtat ccgcttacta aaaagagaaa tagtcgtact ttggcacgaa actttgtgcc
    21661 aaaggtttga gtatgtcagc aaaataaaaa gatttcggta tcagaaaact tcaattttaa
    21721 ataaaactgt tattttaatt tttttataat atgcaaacaa taacttttgt tagcatttaa
    21781 tttagatgtt ttccattcta ttatgttatt aaattttata ttggaggcct atctatgaca
    21841 ttactgatca aaaacatgaa catgaaacgt ggtaaaaaac gtgtgattcg acaattgaat
    21901 cttttagtag aatcaggaga agtagctgct ttgattgccc cgaacggatt tggcaaaaca
    21961 actttgctga atggcattgc ccaactgctg ccaacaactt atgatcaatt gtcccttcac
    22021 aacatctctc ctactgctta tacagattat cgccggtctt tcttttttct agaatcaacg
    22081 aatcagttat ccgactatct atcggctgtt gattatttga ctttgatcaa atactattgg
    22141 cagagcaaag tagaatgcca aactgttttc caacgattga agatgactga gtaccttact
    22201 ttgccgattg gaaaaatgtc tctaggaatg aaacaacatg tcttgcttgc tgcttacttg
    22261 atcagtgatt catctgtcat gttattcgat gaaccgttga acggattaga cccaggaagt
    22321 attgatatct tgaatagcct catcagacaa tggcagaaag aggggaagac gattcttctt
    22381 tcttcccacg ctatcgcgaa tatccaatcg atttgttcta aagcatgttt tctaaaaaat
    22441 ggagaaatcg tcacagaatc ctcaaatatg gaagagattt tagctattta tcgtaaatta
    22501 tatgaggtgt aactatgtct ttacttacat ttgaacgcaa tctcttacta aaaaacaaag
    22561 taaacttttt atttcctcta ttgttggtcg tgctctttgc attcccactt ttttttgatc
    22621 ataaacttgc ttatacagaa ttcgatgaac tgaatcataa ctatgaagag atgcaacgtt
    22681 tgattgaaac gttgaaagaa gatgagaatg agaaagaatt tgtggaaagt ttagagaaaa
    22741 gcaacaaact gatcgaagca atcttacatg caaaaaacac agggaatgtc cagcaaatgg
    22801 tcgaagcaac ttatcattat gaaaaagata tactggatcg actgatttct ggtcaaagac
    22861 aaggaattcc aatcattgaa caacaaaaac gtgtcgagtt gctccgttat atgaaagaac
    22921 atcagatcca aagatattct atttttgact tgccagcgca tctctcttta gcaaattact
    22981 acgaaaatat tttttcagga atgatttcta gttttttgat tttatgtata accgccttgt
    23041 ttctttcttc catcatctct tatgaaaaaa gaaagcaggt aatctctcta gtcaatctat
    23101 tactggatag tatggtaaag aaacatagca tccgttttac catttattat ggtgcagcta
    23161 tgctaagtct cgtgatgcca tttttgatcg tctctatact agtaataatc aaaaatggtt
    23221 tgggagactt ccgttatcct gtaggaacga tcattggtca ggagatacga atcctgccga
    23281 tgtatgagta tcttttccag agctttctgt tcctgttgtt gtgggtgctc tttttaagta
    23341 caatcagttt tcttctatct gctttatttg aacattctct agtcaatctt ttaggtacac
    23401 ttctttgtct tttcttagca gagtatcgct tgttttcttc gattggatgg atagaatcaa
    23461 tcagtcatta cttgccaact tcttatgttg attttcaaaa tgtgatcata ggaggagata
    23521 ttttctcgcc actagcttca gaacaggtta ctttcatgaa tggtatcttg acacttggta
    23581 tatggagtat cgttttatta ttcataggta tgggcactat ctatataaaa aagagctatt
    23641 aaaacacaaa aataataggg gcggtgacaa aaaccttgtc accgtcccct atttcaaata
    23701 aacggttttc tagaataaat agttttcaaa agcttgcatc cgcagtaata agaacctccc
    23761 aacaaaaata gctcctctta tttttatctt ggttgaagtt attacttgat gcaaggcagc
    23821 tttttttact gtgaacattg gttatatatg tccatgttca aaaatcattt ggacttcttc
    23881 aatggtttcc gcatcactga gcgccattac ttggtcgatg tctgaacaga aaaaggctaa
    23941 atcttggagc accgctaact gatgttcttt ttttaaagcc actacaaata agagtgtcgc
    24001 gatttttggt tcctcttttg ttccaaagtc cacaccatca ggcacttgga ctaaatagat
    24061 tccttcttct actacttctt catctttgtc agtcccatgt ggcaaggcta cgaaattccc
    24121 gatatagaca ctcgcttttt cttgtctttc gatcattgat tggatatatg ccggtttgat
    24181 tcccccttgt tgggtcatcc actggccgat ctcggtcaac gcttctttcc aattttgata
    24241 ggattgatcg agcaatattt tttctgctgg tagctgtatc atcttttcac cttcatttct
    24301 ttatttcttc aatcaggatt ttcgctaaca actgataaac gatttcttta tttcctgagc
    24361 ggaagatctc ggtgttcaag tcattcatga tgattgctcc actgatcttt cctaaaagaa
    24421 tcccgtcttt ttcttggatt ggcgaaggcg ccagcataac taacatacgg gacaaatcga
    24481 ctggctgtct atccatccct tcaatggtca acggatgttt caaattgaaa atacaaaaga
    24541 tcggtttaga aatcaatgag ctggatgtat ggaataaccc catatttgta tgaggaatac
    24601 cgataggtgc ttgttcatat cgcttaagca gccggcgatg aaccacctct tcatccgtta
    24661 ctagtccttc aggaaaataa ggaaataagc tgcgaatcgt ttcggaaatc gtctcaggat
    24721 tatccagttc ttggataaaa aaacgaccga gtaattgatt gatttgatcc atgaaatgca
    24781 ttgtttcctc ataagtatct tctatcatcc actcgttatt gctgttatac gtaatttttt
    24841 gccttttttt cttctcaata tgcgaaaaag cttcttttag ctgcttgact tcatcttcta
    24901 gcagtagcgg ggagactaaa atatattttc caccgtatcc tggtaaaata gatgtagaaa
    24961 taacaacatc atattcttct tgtaaatcga ttttttttaa atcagcaatc cgatagaaat
    25021 gaatttgttg gatgaaggga aaataccttt tcaatcgtag ttccaacatg ctagtagaag
    25081 ctaatccact tggcgaaaat cctgcaatac tgatattgct ttctttcgga cttcgttcca
    25141 atgaattcgc aaaatgaagg accatataag cgatctcttc ttcggataat tttttttctt
    25201 gaaaaatccg ctctactgct ttacggattg cattcgcaac ttcctcgtat tgaaccatga
    25261 ttttttctaa aatcggattg gtcagtgtct cttcttgtaa aacggctctt gaaaacacac
    25321 cagataaatg agtcaacagc attttgtaca aagtttcatc ttcataaaaa tggacattcg
    25381 tctcttcact tacttcttga atcagttgtt ttactaaata agcaagctgt gtatcgaacg
    25441 tctcttcaaa gaaattctga tcaaatgagt tcgtgaagtc atttaataat cctgcaaaaa
    25501 agacgatctc atttactgga taaagttgtt ttgtctcaga agcgatccgt gaaaacaatc
    25561 gtttagctgt ctgtagtaat tctttgtttg ccagactatt atatgtctca tttgtcagta
    25621 aatgattttt cgtcacacgc tccatcgtca atgtcagcat caaaaccaaa tgttcaagct
    25681 ttctatcact tagattggtc tttttttgga tcaattcttc cccgattact ttgcgtgcaa
    25741 gagatagatg ttgcggatca acgaatcgaa aaaaataatt cagtgatgca tcgaaagaaa
    25801 tactatgaaa aagttcatat tcattgattt ctaattctaa tacattcgct gtcagaagcc
    25861 ggcggtattt ttccggtcct tcgatctcgt atccctggtt ccgaatgatt tccaaaggca
    25921 gttggcgaat actttcttcc aactgtttga tatctgcata aaaagtggta ttactgatgc
    25981 gatatgattc catgaaatga gcaagactca acggctctgt cgtcagtaac aactcggcta
    26041 aaatcgcgtg ttgtcgttta gcagtggata agtcattact tttcccatct gtattttctt
    26101 ttaattgctc taatacttct tctgatgcct caatgacaaa tgagcctcta gctgcttttt
    26161 tcaaagaagc atttaccgat ttcaatgaga cttcaagatt atctaattcg cggtaaatag
    26221 tccgtttact gaccttcaat aaattcatca ttttttggat agatacaggt gacgggctac
    26281 tgagaaattc tgctaataat tgtttttctc tgggcgataa atacatctta tatttctccc
    26341 ttcacgagtt tttcgtattc attcgtagct acccaatgtt caactagaac tagccgaggg
    26401 atcttttctt ctagctgttc agttacagct agttctcttt gtgcgatata aatcgtttct
    26461 tcttggattt tgattttttc tattgattct tttgagataa caaactgttt tttcgctttt
    26521 tttgctaatt gacttaaaat cgtcatgccc atagtttgtg agccgcgtct gttgttttta
    26581 tacaaaaata caatattagc aggcgacgtt ttttgttctg tgatatctgc tatctctttt
    26641 ttttcttccc gctttggaaa ttggatcgct tcttttattc gtgacagcaa gatatctgtt
    26701 tccaaaaagt gatccagtac atagatttga ctttgaggta ctttttctct tgcaagaccc
    26761 gctagttcat gttgcacgat aaccagctga tttacttggt tattcaattc atagatagag
    26821 ctgtatccga cagaaagatc gattttttct gctgctaatt tctttttcag cagggcggct
    26881 cccatagcac ttgatcccat acctgagtcg caagcaaaaa tgacggaatg aatgatagat
    26941 ggttccaaga tttctcccct ttctttgacc gttttgttct ctttttcttt atttggtaat
    27001 ttcaatagta tcgctgcaca aatgaaggtc acaaatgtac tgattgctat ccctatactt
    27061 accccagcta gttgtggata aggcgcattg gcaaaaatca cgagtagtga cccaggcgat
    27121 acaggtgcag ctaatcccgt atctaaccat tgaaatatca acgtgccgct tgccccacca
    27181 gccattacag ctaaaaatag atacggtcgc agcaagacaa atggaaaata gatttcgtga
    27241 atcccgccaa tcgcatggat catcaatgct ccactcgcat ttactttttc ctgtttttct
    27301 gcaaaacaaa ggatagaaag taagatccct aaaccaggtc caggatttgc ctccatcaaa
    27361 aataaggaag agtacccgac tgtttgtgag aattcgattc ctaaaggtgt caatacacca
    27421 tgattgattg cattattgaa aaatagtact tttagaggtt ctaagatcag actgacaagt
    27481 ggtaacagat tcattgtgac tagccaagac acaacggagg cgagcaacaa gtgaacacta
    27541 tttaaaactg gttcaataaa aacgattcca attatacaaa taacgctacc agtcagtcct
    27601 actagtaggt tgctgacaag catttcatat ccagccttga atttcacatg gaatctagaa
    27661 acaaattgtt catatacgat gccggcaagc ggcccgataa tcatcgctcc tacgatttgt
    27721 ggagcattgg aacgagcgat caaccctaaa gaagcaattg caccggcgac tgcccctttt
    27781 tgtcccccta ccgatttccc gcctgaataa ctgatcaaga tgggtaaaag cagctggatc
    27841 atccaatggc atacctcttc taatgtggtg tgcaaatcgc cagacacacc ttttgttgct
    27901 aatgacaaga ttccccaagc gataaacaga cttagatttg gcatgatcat ttcatttaag
    27961 tgatgtccag cttttctcag ttgtttcata gacacgcgac tgctgttgtt tgctttccat
    28021 ttgtattcca tctttttcac cagacttcta tttattgatg gacggatttt atgtagaaaa
    28081 agcagcaaac gaaaaaccgt ctgctgcttt cagctatctg ggaactattt gcctggttca
    28141 gacgcaatga ttgctagttc accttggacc gcccactcag aaacgccatt tggaataata
    28201 cagcttgttc ctttttctaa tggatatgtt tttccgtcaa cgacaaggtc tcctgctcct
    28261 tcgattaccg tcatcaatgt ataaggggct tgttttttga aagatgtgat tcctttaata
    28321 tcccactcat atacattgaa aaactcagtt tctaaataag tgacgatcga tgaattccct
    28381 tttcggactt ctttgatttg taattctggt gttttcgcag ggacagttgt tacatcgatc
    28441 gattgttgga tatgcagctc tcttgtttgt ccttgcgcat cttttctgtc gtagtcatac
    28501 acgcggtaag tcgtgtcaga actttgctgt gtttctaaaa tcatgattcc tttaccaata
    28561 gcatggatcg ttccgcttgg tacataaaag aaatcacctt ttttaactgg tactttcttc
    28621 aaaaggtcat cccagcgccc atctttgatc atctcagcta gttcttctct tgtctttgca
    28681 tgatgaccat agatgatttc tgcaccaggt tcagcatcga tgatatacca gcattcagtt
    28741 tttcctaatt caccttcatg tttcatccca taggcatcat ctggatgtac ttgtacagat
    28801 agatcatctt ctgcgtctaa gatcttgatc aataatggaa acactggttc tgaaggatgg
    28861 ccaaacaatt ccttgtgttc tgaccatagt tcatctaatc ttttcccttt gaattcgcca
    28921 ttttctacta cactgacacc atgaggatgc gcactgatcg cccaatcttc tccgatttta
    28981 tcgttaggga tatcaaaacc aaagactgaa cgtaaacgac ttcctcccca aattttttct
    29041 tgaaaaactg gttttaaaaa cataggttcc tgcataattc aaactcctct catttttcat
    29101 agctattata gcattatttc ggtaataccg ctttctcttt caaaaagttt tcaacaattt
    29161 catctcgaca tatgagatac tccgcacgat gatcgacagg atgtacgcga tttgacaaaa
    29221 atatcaacgc ttcttgtgtt tttatatcaa tcaagaaaaa cgtacctgta taaccagtat
    29281 gaaaaagaat cgcatgaccg tcttctacat ccttttttaa gtcccatcct aaagaacgat
    29341 agccattttt tttcggtgtt tgatcttgca gtaaagaatc aatcgtctct tcttctaaaa
    29401 aacgaccatt acggattttt cccttgttca aatacatttg gacaaaagta atgctgtctt
    29461 tcaaatcaat aaagagaccc gcgttacctg catgttcagc caaaactctt gctttcggat
    29521 catgagtttg tccacgcaaa acctcacccg aaggcagttg ttctgtggga acgatttttt
    29581 tggaaggaaa aggaagaaat aagctgttca tcattcctaa tggttccaat acttgttctt
    29641 gaaagatttc cactgcagtt tcttggtaaa tttcctctag catcaaccca agaagaatcg
    29701 taccggcatc tgtatatttt acttcttttc ctagttgctc acctggttct agcttcaaat
    29761 aagcggcgat caattcttcc tgagatagct tatctctgtt tggtatccat gttttgatat
    29821 cagaagtatg tgtcagtaaa tgacgcaaag tgattgtctg attttcaaat tcaggaagat
    29881 atttatgaag cggttgatcg agttctatct ttccatcttc taatagacgt aaaactaatg
    29941 aagtggttgc aacgactttc gtcagtgagg ctgcatcaaa caacagatct tctgtcatca
    30001 gctgcttttc aggaacgact tgggcaaagc cggctgtaaa tgtttctact tgattttctt
    30061 ttatgaaggc gactgctaca cctggataaa atccttcttc catctttttc aaaataagct
    30121 gtttcgtgtg tggatacatc tttttctcct gcttttctat cagattctta cagtccaagc
    30181 ttaaagctta atctatgagg aatcaacctt ttttacacaa aaaaactgcg cgaaaaaccg
    30241 cgcagtttcc cccggtatac tattttctct ttctatctct agaataagcg atagtatgac
    30301 cgttcataaa atgacgagac atgcgacaat cgttaagaaa gaaaaagtta agcgtcgcat
    30361 cgaatgtcag ttttattctt ttcttttggc tcactgacga accaccattc gattcctata
    30421 gcatcataga ttgtgagtac tgtgtgcttt ttgtctaaga agaaatcaac tgatagttgc
    30481 tgcaagtgag caatcagtcc ttccatgctt tcagtatcga tcatgaattt cagaaaatca
    30541 agtccaatca cttcatctgt aggcaaatca attgcgcctc cgctagcttc tgtcaaacca
    30601 acatggaaat ctccttcatt caatacatgg atgccctctt cttcattatg taccttcaaa
    30661 ccaagtacat gattaagaaa ttggcgttct tttgttaagt tagtaacatt taaatggatt
    30721 ttttcaaaat aagaacctgt tgatagttta ctgaattttt cttcagattt tcttaataat
    30781 gcttcttgat ccattgtttc tggaatttct ctttggtcgt tcttattctc ttcatggtaa
    30841 atttccaatt cattctcttc tgggtctacg agcaaaatcc ccatacgtcc atgatcttct
    30901 aaagcttctt tgattggata attcatttta cggatacgac aaaggatatc tgccatttct
    30961 tctgtgctag ggataatcaa tgaaaaacgg ttcagctttt tgatcacccc catgtgatca
    31021 ttcgctctag gactttcttc cagcagcaac atttcacttc cagcttcttc tgttcctaaa
    31081 atggctaatt cgttttcttc tcttttcaaa tcaaagccaa caacgttctt gtaaaaatca
    31141 atcattttat ctcggtcttt cacccgaatg gctacacttt ttaaataggt ggcagccccc
    31201 aattgaaaat ctttcatatt tcttgctccc ctctacgtca agctttattt taacgatgga
    31261 ttcttcttct ttcaagcaat aactttgatt ttgttaaaaa aaatgtgaga aaattcgcat
    31321 tttgcttgtt ttttgtaaat taatgagtaa aaactcttat aaaaagaggt tgtgaaaaag
    31381 ctgttctgca tcaagtaata agaacagcca aacaaaaata gctcctccta tttttcgttt
    31441 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    31501 aaagtcgctt agtttcgagt agtaagaaaa attttgtgaa aatggctttc tacatttttg
    31561 cataaatttg gcttaatacc gagaaactgg cttttgaaca ccgtttacca agaggttgtg
    31621 agcctgccgt ttaactccga gtcacaagga gcaatttcaa aaaacagctt ctcaggtttg
    31681 ttgactattg tgacttgtga ccgaggagtt ggcaggagaa caccgtttaa ggaagaggtt
    31741 gtaaaatcag cggtttgacc tgagtattag agatacaaaa cggaaaaata gcttctcata
    31801 ttttgccgtt ttttgagcta ataccgaagg acagctgatt gaacaccgtt tattcggaaa
    31861 aagggactgt gacacgactt ttgtcacagc ccctaaaaaa aataataaat ttgtactatt
    31921 ctatgaagct tcttcttcag aaaactgact gttataaaga tctgcataga atccgtcttt
    31981 ttccatcaat gtatcgtggt ttcctgtttc tacgatcgaa ccatgattca tcacaataat
    32041 gttgtctgca tcgcgtattg tagacaagcg gtgagcaaca acgaaacttg tccggttttc
    32101 caataggcgg ttcatcgctt tttgaatcag tatttctgtt cgtgtatcta cactagatgt
    32161 tgcttcatca aggatcagga catctggatt tgctaagaat gcccgcgcaa ttgtgattag
    32221 ctgacgctgc ccttgcgaaa tattgcttgc atcctcgttt aatacagtgt tatatccttc
    32281 tggcaattta cgaacaaaat cgtctacgtg agcagcttta gcagcctcaa tgatttgttc
    32341 gtctgttgca tcgtcgttcc catatttgat attgtcatag atacttcctg taaacagcca
    32401 agtatcttgc agtaccattg aaaactgtgc gcgcaattca tcacgtgaaa gatcacgggt
    32461 atctacacca tcgtaacgaa tactacctcc agaaacatcg tagaaccgtt caagaaggtt
    32521 gatcagtgtc gtcttccctg caccagtcgg acctacaata gcgaccatct caccaggttt
    32581 gacatccaag ttaaaatcag tcattagcaa gttatctggt gtgtagccga attgaacatt
    32641 ttcaaaacga actttgtatg gtgtatcttt ttcttctgga aggccagatt tttcattgga
    32701 catctcttct tcatccaaca cttcaaatac ccgttcggct gaagcaactg ttgattggat
    32761 agtgttcatc aaacttgcaa gttgagtgat tggttgagag aactgtgttg tatattgcaa
    32821 gaacgcttga acatccccta atgtcatgtt cccatttgca acttttactc cgcctagtac
    32881 agcgacgaac acatagccta gatttttaac gaaattcatc aacggcatga tgattgccga
    32941 aataaattga gctttccaac cagcggcata cagattctga ttttcttttt caaatacttc
    33001 ctggtctttt tcagtatgat tgaatgtttt aacaatggta tgaccaccat atgtttcttc
    33061 tacttggtta ttcaataaac ctaaactttt ttgctgatct gcgaaaaaca cttgtgattt
    33121 cggcgctacg atcatcacga tgatcaagct taaagggatc atcaataacg cgactaacgt
    33181 caattgccag ctgattgaaa gcatcatcac gatgacccca atcaatgtga ctgcactagt
    33241 tacaaattga gtcaaatttt gctgcagcgt gcttgcgatg ttatccatat cattgattgc
    33301 tcgagacatg atatccccat ttgtatgtgt atcataatag gaaatcggta cacgattcat
    33361 tttggcttcc aaatcatgac gtaattcata aactgttcgt tgggaaacac gtgtcataat
    33421 aaattgctgt aaaaagttga atacagcaga aatcaaatac atcacgatta ctgtcgcaat
    33481 gatctgcgcg attttatcaa agtctatcgg aaattttgtt acttgttggc cttgttgcat
    33541 ctgttgagcg ccagccatta cacctttgaa tatttctgtg gttgctttcc ccaagacctt
    33601 tggtgtttgg atttggaaga tcacggctgc tattgctagt acaaatacag cgatgatcgc
    33661 aaccatacgt ttagacatat agccaaacaa tcggcgtacc gttccccaga aatttttagg
    33721 cttttcacct tttacaccga gatttctgcc aggtccacca tgtcctggac cgcctgctgg
    33781 tccatgagag ggacgtgtgt ttgttttact catgctaagt cctcctctct tagttgtgaa
    33841 tgaacgattt cttgataagt ttcgtttgtt gctaatagtt cttcatgtgt tcctttgcca
    33901 accagttttc cttcatctag cacaaggatc atatctgcat ccattactgt acttactcgt
    33961 tgagcaacta aaacggtgat actttctttc atattcgttt tcaatgcttg gcgaagattg
    34021 gcatccgttt tgaaatccaa cgctgagaag gaatcatcaa acacatagat atctgctgtt
    34081 ttgactaatg cccgtgcaat tgccagacgc tgtctttgtc caccagagaa gtttcctcct
    34141 ccttgttcca catggctatc taagccatct tccatctctg aaacaaaatc tttagcttgt
    34201 gcgatttcta atgctttcca aatcatttca tctgttgcat ccggcttacc atactgcata
    34261 ttgtctcgga tcgttccaga gaacagaact gctttttgag gaacaaagcc cattaattct
    34321 cgcaaattat attgagtcat gtcactaatg tccgttccat ttatttggat cgaaccagat
    34381 tcgatgtcat ataatcgagg gatcaaattg actaaggtcg ttttacctga accagtcccc
    34441 cctatgattg cgacgatctc gcccgacttg gcttggaaat caatatcttc caatgcaagt
    34501 ttttctgctc catgatatcg ataattgaca tgattgaagg ccaatgttgc gttttttcca
    34561 gcaaaagaaa ctgttttagg attttcaggg tcatggatac cgattttttc atccaacact
    34621 tcattaatac ggtcagctga agcttgtgca cgaggcacca tgataaaaat agcggaaagc
    34681 atcataaagc tgatcaagat ctgcatcgca taagtcataa atgcaatcag attaccgact
    34741 tccaaagtca tatctgctat gtagtgtcca ccaaaccaag tgattgctac gtttgtaccg
    34801 ctcatgatca atgtcatcat tggcatcatc aatgcgacga tcgtattaac tttgattgcc
    34861 gtatttgtat aatctttatt cgctaaatca aaacgatttt cttcaaagcg tgtcttatcg
    34921 aatgctcgaa tcactctgac ccctgtcaat ccttctcgaa ataccaggtt taaacggtca
    34981 gttttctttt gcatactttt gaataatgga acagcaaagt acatgattcc gccgatcaaa
    35041 ataatcatta ctgggatgac aactaagaag atttttgtca actgatgatc tttttgataa
    35101 gcaagtatgc tcgcgccaat caacgtgatt ggtgcattga tcatcatacg taaaaacatt
    35161 tgtgtaacca tttggatttg agtcacatca ttggtcgttc ttgtgatcaa agaggccgtt
    35221 ccatatttat caaacgcatc ttttgtcaga ctctctgatt ttttatagac atccgtacgc
    35281 aattgcttac ccagtttctg tgattcacga gtagcaaaaa atgtatttgc gatagcagct
    35341 aagatactga tcaaagaaaa gccgatcatc acaaaacctg tatgccaaat ataatcgaca
    35401 tctccttgcg ctaccccttt atcaataata ttggaggtaa gtgtcggcaa atataaatct
    35461 gccagtactt ggatcaccat gaaaactgca gctgcaattg ctgaagtcaa ggagatccgt
    35521 tttacgattt ttagcatcta gttcccttct ttctatttat atactccgtt tttcagccaa
    35581 gaaagtttca aattgaattc actgattgct tgctctactg agtaatcttc agatgttgaa
    35641 aataagcgat acgaatgcgc aattgttgta aatacagtat tcatcactaa ttggatcagt
    35701 aattccaatt catgatcatt ttttactttc agcttcgtcc gatcgatcaa ttcaagcaac
    35761 tcttttgcat cttgtttgtg ttctttatgt ggatcgtgag gcgttttttg gcgactcgta
    35821 tgaggagcga cttttcttga ggcacggtaa tccatgttca taaacaagtt acgataaaaa
    35881 gaagcattct cgccttccaa aatctcataa atcattttag aaaaatagga ttcgaatcct
    35941 ttgaatagat caccatcgga ttctttcatt agttgtaaca tatctttaga gctgtcacgt
    36001 ctcattgtct caaagtaata aaaataaaga tcctctttat cttcaaaata ctgatagaaa
    36061 ctgcctctag ggatatccgc taatttgacg atattagcaa tcgaagcttc ttttaagggt
    36121 actctagaaa actcgattct cgcagcctcg attagtcttt tttgtttttc tttaggcaaa
    36181 tggaaaaatg tctgtgtcgg catatggatt ctcctttctt tcaagatgac acattgtcat
    36241 atgacgctat gtcattataa cacctcctac ctgttttgca agacatttgc atagtttttt
    36301 tcattatcat ccgatacatt aaaaaagtga taagaggaat ccttccattt gaattaatga
    36361 gagttgccca ttaaaatagc cacatactaa acaaaggatg agataaatgg cccaatcaat
    36421 tagtgtccaa gacttcaata aattgcctct cgatgctgac agcatcgttt tagatacaag
    36481 aaatacctta gattacacaa aaaaccactt aagccactcg ctttctatcc ccgctgctat
    36541 cttgccaaga caattaaaaa atctagatcc aaagaaactt tattatattc taagctatac
    36601 aggcaggcga tcagaagcat tagccgatca acttactgct aatggttttc gtgctgttta
    36661 tatcattggt gggatggaag ctcttcagtc gagtgttgct tgaatattgc ttgttagatt
    36721 caaaagaggc tgtgacaaaa aatcgtgtca cagcctcttt tataccctgt ctttttttag
    36781 ttatttattc ttcaacctta tcaatatgac ttgatagcct tttctttttc gagtaaactg
    36841 attttgttag tcatagttat aacaatcata gcaataaaag gagaaggtaa gcatatggca
    36901 tctgttttag tagtcaaagg tcatccccta actgcagaag aatcgcgcac agttaaggca
    36961 ttgacgtctt ttttaacaag ttataaagaa aatcatccag atgatgaagt gactgttttg
    37021 gaactttatc gcgatgatat tccggaaatc gatgaagaat tattatcagg ttgggaagca
    37081 ttacgtgctg gtgcagaatt cacttcctta tcagaaagtc aacaacaaaa aattgctcgt
    37141 ttcaatgaat tgactgatca gttccttgct gctgataaaa tcgttatcgc aaacgcctta
    37201 tggaatctaa atatcccaac aaaattaaaa gcttggttcg ataccgtcaa tgtagctgga
    37261 aaaacgttcc gctatacaga aaacgggcca gaaggattag tgacaggtaa aaaagcactg
    37321 cacatccaat ctaatggcgg tgtatacaac ggacaagact ttgcctctca atatgtaaaa
    37381 ggtattttaa actttgtagg catcgatcaa gtagatcagc tattcatcga aggaatcgat
    37441 tatgaccctg accgtgcaga tgagttgatg caaaatgcat tagataaagc tgctgcttta
    37501 ggtaaatcat tttaaaaatt gagataaatg catacaaaag agacagagga aaaaatcttc
    37561 aaagcagcat ttgtgccttg gatattttcc catctgtctc tttttgttat tctttctgct
    37621 ttttcaacgg attttcttca taaagagaag agattgttgt gtaccattca aaaacatgag
    37681 taatcactac tttagctgct gcataaacag gaatccccaa gatcacaccg atgattccga
    37741 acaatttccc agaagtcagc agaactaata aaatcgttac tggatggatt gctaactgat
    37801 tccctagtac taatggagag atcaagcgtc cttctattgt ctgctctact gcaaaaacga
    37861 tcagtacttt gatgatcatg aatggacctg ctacaatggc taaaaagata gccggaatca
    37921 tcgctaaaaa tgatcctaag taagggatca ggtttaaaaa acccgcaata atccccaacg
    37981 tgacagcata ctctaaacca atgaccgcaa aaccaatcat aaacatgatt gccacagcaa
    38041 aagccacagt tagttgtcct cttatataag aagaaacttg actattcatt tcactcagca
    38101 cattcaacgt cggtttttgc atgcgtgtcg gtaaaaattt gacaaaataa ggtgctaatt
    38161 gtttaccgtc tttcaataga taaaataaga taaacggcat cgtcagcaga gccactaaaa
    38221 tagtcgctac cgcgccgaca aaacttccaa gactctgaac tgtactctta gatatatctt
    38281 ggatcatatc gcctgccgaa ttcatgaact tatcacctgc tgtttccaac tgttcgcgaa
    38341 attggttgaa cagcgggtcc cgcaaaatct cggttaattt attatcaatc gtatctacat
    38401 attgagggaa gttactgatg aagctcactg tttgttcctg aatcttaggg atgatcacta
    38461 cgctccccca tgcgattagt gcaacaacca aaacaaacag cccaatgata ctgtaaagcc
    38521 ttggtacttt tcgcttttca aagtaatcaa ctaccggatt catcaagtaa tacaagattc
    38581 ctgctaaaat gataggcaga cctacgattg ctaaaaattg ccagattggt gcaaacaaat
    38641 atgaaacttt tgtaaataaa aagacgatca gcaatattaa caatacaaca agtaaggctg
    38701 tgactacttg attatttaaa aaccagcgcc aaaaccacga taccggtctg ttcttcttat
    38761 tttttccttc cataattcct cctgttagtt ataaaaaatt tggcttccca aaccaatagc
    38821 aggcagtcta tgcggatgaa gatagattcc atttgcaatt tgtccttctt ctggacaagc
    38881 atcaaaaatc aatgatacgt gagctactgt ctttaaattc ccattggcca tcggaccgac
    38941 ataatcgatc gtatatacct gatcatcaaa agcaattgtt ccgcctgctt tcaaagaaaa
    39001 aggttcgtct tttgatactt cttgaatcac cgaatatttt cgtaaggcag ctgttgctgt
    39061 tttatcgaac aaaatcagca tgggttcttc cttgtttatt gcttctgtcc caatttctgt
    39121 aatctttgcc tgaatcattg cactctctcc tttaattgct ttcaaaaaca gtataacaca
    39181 ttctcattct ttagccgtgt ggaataatta tgtgatagaa taaaaacgaa aactatttca
    39241 ttacaattag gaggaatcag ctcatatgat cgaaaagata attttaggaa cctatacgcg
    39301 tcgcgaaagt aaaggaatct atacgatcga ccttgataca gaaaaagaag aattagccaa
    39361 cctttcatta gctgttgaag aaaatagccc tacctatgta gcaaaaagta aagctggcaa
    39421 tctttacaca gttactagtg tggatggatt aggtggtgcc ggtgcgtacg ataaggattt
    39481 tcattttttg aatgcggtaa cagagtctgg cgctcctttg tgttatgtag cggttgatga
    39541 agcacgtcaa ttactatacg gggcgaacta tcataaaggt gaagtaaatg tctaccatat
    39601 attaggagat ggcgaactga aagcagcaga ttcaatattc catcaagaag cgacaggtcc
    39661 tcataaaaac caagatcatg cgcatgtcca ctatacggat ctgactcctg accaacgatt
    39721 agtcgtatgt gatcttggta cagatcgtgt gtatacttat gatgtgtccg aaaacggcaa
    39781 attgaatctt gtaactacct tcactgcaga gccaggaaca ggccctcgcc atcttgtctt
    39841 tcatccaaat ggacaattcg cttatttatt tggtgaatta gacagcacag tccgcgtgct
    39901 ttcttataat aaagaaacag gtgcttttga agaattacag aaaatatcga ccttgccaga
    39961 agaattcgac ggtgaaaatg gaggggcagc tatccgcatt tcaaatgatg gcaagttcct
    40021 ttatgcttct aaccgaggac ataactcgat tgctgtattc gcagtttttg aagatggttc
    40081 ccacattcaa aacaagcaaa tcatctcaac agaaggggat tttccccgcg atttcgcact
    40141 agatccaagc aataattttg tcgtgtgtgc caatcagaac accgataatc tgacattata
    40201 tcgccgtcat cctgaaacag gattactgga attactgaaa aaagatgtct atgtaccaga
    40261 atgtgtctgt gtttattttg atcgttagta agaaaaggag aatgaaatga atcagcaaga
    40321 cgtattagac agactacggg aagaactaaa tattcctttc tttgatggga aaatcgaaga
    40381 taaagagtat agcgaagagg aatatcagaa actcaaagcc gatttacaga actactttga
    40441 acaatatgta cagaatgtag aaaactaatc agctttccat actatctgaa aagaacatca
    40501 ggacaagact tttccgtcta tcctgatgtt cttttctaca aataaaaaac cgccgagatg
    40561 gttagactcg acggaaaagg agtatttact cgattttgag taagtacaag gttgttgttg
    40621 gttataggta tgtcattatc ataaaggatt ttgatcactc tatcataaag gtaatattac
    40681 atttatatta gataactcta gtttttattg ttatcaaaga tttcttttat tttttcaaca
    40741 acaaattagt cattttttgg atggcagctg tcaattggtc tgtcgcttgg tcttgttttg
    40801 cttcagatgt attgaataga tcttcatcta cccctgattc aagatcgata ctttggatca
    40861 tttcttcaca gcctgctaca tatgaaagat aagctttttc aaaattttta tgaatgccca
    40921 ttgcttttgc tggtggacgc aaagtactga ctttcataag cattgcccga tatttttttg
    40981 ttccttcttc aaataacagg atgatctctt caagagtcga tttgtccagt tcacttgttt
    41041 ttccttcatc aattgcgttt ctgatcactt catatttcgg atgcattcct tctccgattg
    41101 cttctgtttc cttgacgatt tgatcgatgg actgcacata atagccaata ttcggtaaca
    41161 tgttcgtttt cctactttct ccattgttgt tattatttat tctacttgtt tttatcctca
    41221 aatgacaagt acttgaaaaa atttaatggt ttctatacag ctcgccattc ttattcgtgc
    41281 tcaaagaaac catctgctaa actatattgt ttccctgcga caatgtcata ttggatcaat
    41341 tgatattcgt cgtaaatctc ttgttgcgag cgattgaatt gcgcttcttt ataccactgt
    41401 ccgttttgat agtacagttc tcgttcaaac gccggaagtt cctgttctaa tgataaaagc
    41461 agctgataaa agccatttgt tgtctgattc gtctgttcca ttagattcgc tgcaaaataa
    41521 aacggactgg tgattgcatc gtgagtcgtt tgcttttcta attctccttt actgtcgaac
    41581 ataagaaatt gtgtttcatg caatgtttgt ttgtcatttt ttgcttggat cgtatctgaa
    41641 tagatccctg gaagatgatc cccccaaaaa acaactaaag tccttctaga aagattcttc
    41701 aattcttccg tgaactgctt cagtgccgtg ctgctatagg aaatatcttg cagataattc
    41761 tctaagtcaa gtgtcccact tccatctgaa gtttttgcag gatagtctag attatcatac
    41821 tttccatcgt agggcatatg tgtctgcatc gtgactaaat gaatgaactg aggtgttttt
    41881 tcttccttga gaagtgtcat tacttctttg taagctgact catcagaaat atatggatta
    41941 ttctctatcg tatccgtata ggtcatcgta cgctcgctga tgaactggtc aaatcctaat
    42001 gtctgataaa catcctctct tttatacatc gaagtattgt atggatggat cgctgtcgtc
    42061 tggtatgatt gtgcgtttaa cgctgaaacg atagaaggaa gctgatccat ttttggtaca
    42121 agcatcgtgt aaggggtggt gagttgcgca ttaaataacg ccatagaaaa accagtcaat
    42181 gcttcaaatt cgatatttgc tgttccaccg ccatagtttt gagaaagcat attgccacta
    42241 tatgtttgat cagctacttc ataataatct gccaatggct caccagatac ttctacgccg
    42301 tttaatcgac taggatctga gaaactttca ctcatcacga acacgatgtt cggcgactct
    42361 tcctctacag ttttattttt ttcatcggct agcttctggt atttttcagt gatttctttg
    42421 atcttttctt tcgaatatcc ttcaggttca tccattggtt ctaccttcag attgtacaaa
    42481 aacccaccga taaaaccagt gttatagtag ttcattttct ggctgtaagg tatccatagt
    42541 gccgttttat tatatgcttt tcgcaacaaa ttatcgggat tgttgaaatt actgatatac
    42601 cctagcagtc caattgccac aagcagtgtc gttaatcgga tcaattggaa tcgccttcct
    42661 tttttcaggc ttcgaaacat gtaccagcaa aacaatccta tcacggttcc tgcggcagct
    42721 aagatcactg taaacagcat agtgccggtc atatctttca gcaatccgat ttcagtgatc
    42781 atcttcaaat cgtctggata aatcggttct tgccgataac tcattttcat ataatttgca
    42841 aaacctaata caccgatggc tacgacatac gtaagggaac ctaaagggac agatcctgct
    42901 agtgcaatca gaaaaataag aataatcagc aagacgagac aggctaagaa gaatttttcc
    42961 gtatgccaag aaaaagcaaa tttcaacgca aggtctacgg atagatcatt ttgacaccac
    43021 tgcaaaaata aattactaaa aacaacaaga aaaaagatac tgatgattgc tgctgttcct
    43081 tttaaaatct ttttctgttt ggtcgaaagt ctgcttatat atcttttcaa atgcttgctc
    43141 cttcatttct atcaataaaa gaatgttgct tctcttattt tagttgtacg aaattcgaaa
    43201 gtaaagaacg gaaagaagaa ttaatgaaat ccaaatattc tctttcttta tggcctttct
    43261 ggtttcttta ataagcggac ctttcgtact ccagaaattt tttctagctg ttgtttcact
    43321 tgattgagct gttcaaaatc agtactgtct atgtcaatca atatgtagac atgctcattc
    43381 ttatggttac gcgatatatc tgcgatcgct agctcgttct cattcagcag agaaaatatc
    43441 tctgctaata tattggtttt cttttgataa aatatagtga atcgaaacgg cgcttggaaa
    43501 aacattcgtg cggatggata attgaccgct tctctaaccg taccgaacaa aagataatct
    43561 tttaaggctt caactgccaa acgtccgctg tctgccaaag cttcctgagt cgatccccct
    43621 agatgaggca acataagtat acggtcattt tctaagaact cttcttctgg aaaatcagtc
    43681 aagtatttag ccagatgttg tttttccaat gcttgcataa cagcatattt atcaacaatt
    43741 tcacttcttc ctacattgat aagtacagca tcttttttca ttttttctat attcgcctga
    43801 tcgatcaatc cttttgtatc ttctgtcaac ggcagcatga ctacgatgta atccgaggta
    43861 gaaagaactg tttctaaatc tgcttgccga acataatcca actgggcatc gcggatagaa
    43921 taacccaaga catccatccc taagctatag caacttagcg ctacttttgt gcctatcgct
    43981 ccaagaccaa gaagaccgat ggttttgcct tgcaattctc gtcccacata agcacttctt
    44041 ttgttttctg cctgttctaa aatattagga cctgttagtg tttgtaccat acgacttgct
    44101 tcgattatcg gacgggaaga taagagcaaa cagcacaaaa caagctcttt tactgcatta
    44161 gcatttacac ctggtgtatt catgacgatc gtcccgtttt cagaagcttt ttcaacgttg
    44221 atcgtgttca ccccaacacc agctcttgaa attgctagca aatctggtgt aagccattca
    44281 tctttcactt tcgtactacg aataactatt ccttcggctg gcaaatcatc tattattttg
    44341 aaatattccc cttgtaactt ttctatctct tgttctggaa tagcatcaat caaatgaacg
    44401 tgatacataa aaccccctcc ttactaacaa gtatctcgaa atttctgcaa ttagacaact
    44461 gtttaccatt tgacttccta gaagcaaaaa gagcagttag agcagagtag gctgagtgat
    44521 aaaaataagg ctatgacaag ttactgccat aagccttatt ttcgagagca aaatattgag
    44581 ggtgagatat cttgataaat ggtgttccgt tagctgacat tgataataag actaaatttc
    44641 aaaaaagttg aaaagcaaat ttcgtcaatc tcttcttatt tcttaaaact aagtggcttt
    44701 ttcacaacct ctttttatct aataaccact gtttccaact caactaattt tgcccacgtt
    44761 tcaatttgat ctgttgttag atttagcgag acaacggtat gatgaccgcc acctgcctga
    44821 atccatgaat ggacaccttc atggaaattc ggtttagttt tccataacac acgtgcaaca
    44881 ggtaaattag gtgcttcagt cgttggttca aaggcttcta cttcattgat cagcagttta
    44941 taatgcgtac cgaaatctgc catcgatacg actacccctt ctcctgcttt cccatcaaat
    45001 acaagacgcg ccggatcttc tctatctccc attgataaag gagaaacaac gattcttggt
    45061 ttcgtcgcag caagcgtcgg atctacttcc atcatatggg attccaagat tgcttcttgg
    45121 ccactagcta gctcgtaagt ataatcttcc atgaatcctg tatctttccc tcgagccatg
    45181 attttcagca accgatcaat cgctgcagtt ttccagtcgc cttccccagc aaaaccgtat
    45241 ccttctgcca tcaaacgttg tacagctagt ccaggtagtt gtttcattcc atacaggtct
    45301 tcaaaattag tcgtaaaagc gttatagcct ccttcttcta agaaacggcg aataccgatt
    45361 tcctgctgag cttgtacttt tacatgttcc tcccatgtct tttcttcata atcaccatag
    45421 tcgaaggtat acaattcctt gtactcttca aataattgat cgacttcttc gtctgatacg
    45481 cgatcgatta cttgtacaag atctccgata ccaaaataat caacagtcca accaaattga
    45541 atctgtgctt ccactttgtc tccttctgtg acacccacgt ttctcatgtt gtcaccaaaa
    45601 cgagccactt ttattccaaa actttcatta taggctactg caacatccat ccaatcagca
    45661 atctcttttt ggatctcagg tcgtttccag tagccaacga cgattttatt ttgtttattt
    45721 aatcgagcat tgatgaaccc atattcgcgg tcaccatgag cggattgatt taaattcatg
    45781 aaatccatat cgatcgtttt ccaaggaatg ctttcgttat attgcgtagc taaatgaagc
    45841 aatggttttt gtagaagctt tgtcccgcgg atccacattt ttgctggtga gaaagtatgc
    45901 atccaagtga tcacacctgc tacttcttct cgataattga cctctttcat gatttttgtg
    45961 atcgtatcag cagtaactgc taattcttga aggcggattg gataaggcaa ttggccattt
    46021 tcattcagtt catcgaccat ttcttgtgca tgtttcttta cttctcgcag tgcttcttcc
    46081 ccatataaat gctgtgatcc tacaacaaac caaaattctt tttctccaat attcaacata
    46141 attagccact ccttttattt gttttgtccg taatacgcat tttttccgtg ttttcgtaaa
    46201 taatgtttat ctaaaatccg ttgaggcagc tcttctgaaa atgaattgag ctgtctggta
    46261 aataaattca tcttgcatac ttcatctagt acgactgcgt tcattaccgc actttgcgca
    46321 tctttccccc aagtaaatgg accgtgtccg tgtaaaagta cccctggaat tgctaaggga
    46381 tcaattttgc gttctttgaa tgtttctatg ataacttttc ctgtttcata ttcgtacccg
    46441 ctgtcgattt cctgttgtgt caaaaatcga gcacaaggta ccgatccata aaaagtatct
    46501 gcatgcgtcg tccccatcgc aggaacatcc aatcctgctt gtgcccagat cgtcgcccat
    46561 gtcgagtgag tatggacgat tcctccgatt tgaggaaatg aacgatataa aacagcatga
    46621 gtaggcgtat ccgaagaggg attgagttta ccttcaatga tatgattgtc caagtccaca
    46681 acgaccatgt cgtctgctgt catctcttca taactgactc cgctaggttt gataacgaaa
    46741 tatcgtgttt caggatcaaa agcgctcaca tttccccatg tatatttgac gagtccgtgt
    46801 ttaggcaagt caagatttgc ttggtatact tcttctttca gttgttccag cattttctat
    46861 cactcctttt tcttgcaatt gatgaatcgc tgttttttca atgtcgatcc cctcacggta
    46921 acgtctcata aatatctgat agcctgctat ttcttctgct gaaggtgtga tggtgattcc
    46981 ttggtcttgt ccaaacacgt cttctgccaa gaagttttct aaagacttcc catgtcgatc
    47041 catcatatag gctgctaata acgcgattcc ccacgctcca ccttctccgg ctgtgtccat
    47101 cactgtaact ggtgcctcca tagcagatgc tagaatccgc tgtccaactt ctggtgtttt
    47161 aaaaatcccc ccatgcccca ctaaacgatc aatttggaca ttctcagatt tcaatatatc
    47221 cattccgatt ctcattgcac caaaagcact ggctaaatgg atccgcatga aattcgctaa
    47281 atcaaaacgg ctacctggtg tacgtacaaa taatggcctt ccttcattta ctcctgtaat
    47341 gttttcaccg gaaaaataac cataagaaag taatcccccg caatccgtat cgccttctag
    47401 cgctttgttg aataataccg tgaataattt atctctgctt atctccatac ccaaagaatt
    47461 ggtaaattct tcaaaaatac tgacccaagc attgatatcc gaagagcagt tattcgtatg
    47521 aaccatgcct actagttctc ctgaaggtgt agtgaccatg tcgatttccg gatggacatt
    47581 ttctagttct ttttctaata cgatcatagc gaaggcggaa gtaccagccg aaatgttccc
    47641 tgtccctttg gctacactgt tcgtagcgac cataccggtt cctgcatccc cttctggcgg
    47701 acaaaccggg attcctggtc tcaaatttcc cgtagggtcg atcagataag cccctgattc
    47761 gcttaacttg ccagcttctt ctcctgctac tagaacttta ggcaaaatgt gatccaagtc
    47821 aatcgctacg tgcgcttttt cgattaactg accaaaaata tttttcattt cttgatggta
    47881 ggttttcgtt gaaggatcga taggaaacat gcctgatgca tctcctacgc ctagtacttt
    47941 ctcaccagta agctgccaat ggacataacc tgcaagcgtt gtcaaaaaat taatttcttt
    48001 cacatgttct tctttattca aaatagattg atacaaatga gcgatactcc atctctgcgg
    48061 aatattgtat tgaaattttt ccgttaatat tcgttctgct tcttcagtga ttgtatttcg
    48121 ccaagtacga aatggtacta gtaactgttc cttgctatca aatgctagat aaccgtgcat
    48181 cattgcactg aacccaatcg acccgattgt cgtaagattc tcgccataga tttcgaatac
    48241 ttctgctgcc atttttcggt aactgacgcg cagtcctttc caaatatctt ctaatgaata
    48301 ggtccagata ccattttcta gtttgttttc ccactcataa ctaccagcgg caataggagt
    48361 aaaagatgaa tcaatcaata cagctttgat cctagttgat ccaaactcaa tccctaaaga
    48421 tgtacgacca tctatgacat ctaacatact ttgcgcattt acttccttca taatgattta
    48481 actccctcct tctcgtcaaa cctgatcaaa ccgtcttttt agtagcgctt acaatgatta
    48541 ttgtatactt ttgtacgtac atgtcaagca gttatttaca agaatacaat aaaaaacact
    48601 tgttttgtac gtacataagt tataaaatga aattgcttac aaaatctgat caaccgcctt
    48661 ctcgaaattc tagaaggaga gaatacttat gaaaaaagaa aaaaaagttt cctcatcgtt
    48721 tatttatttt tttggcgcat ttggcggcat tctatttgga tatgatattg gtgttatgac
    48781 cggcgcctta ccttttttac agcatgattg gggtttggca ggtaaagctt ctttgatagg
    48841 atggatcacg tcttcggtca tgttaggtgc gatccttggc ggctctttat ctggtcttct
    48901 ttctgataag cttggacgac gaaaaatgat tcttctttct gcacttattt ttatggctgg
    48961 ttctgtcctt tctgcatctg cccctcacaa tggttcttat tttctgattg cagcaaggat
    49021 tcttctaggt ttagctgttg gcgctgcatc tgccctagtt cctgcttata tgtctgaaat
    49081 ggctcctgct agactaaggg gaagattgtc agggatcaat caagtgatga ttgcttctgg
    49141 catgctcctt tcctatgttg cagattattt gctaaaaggg ttgcctgaaa cgatggcttg
    49201 gcgcgtgatg ttagggctcg ctgctgtccc cgcgctgatt ttatttttcg gtgttttagc
    49261 tctaccagag tcaccgcgtt ttcttatgca atctggccgc ttggaagagg caaaaagagt
    49321 attgaactat attcggacac caaaagaagc tgaacaggaa ttcgaacaaa tccagctgaa
    49381 tgtcaaacaa gaaaagacaa ccggaacttc ttggcatact ttgtttttag aaaaatatcg
    49441 ttccttagtc attgccggta ttggtgtagc agtattccag caatttcaag gagctaatgc
    49501 tattttttac tatattccgc tgatcgtaga aaaagcgact gggcatgcag cgagcgatgc
    49561 attgatgtgg ccgatcatcc aaggaatcat tttagttgct ggttctttgc tctttttggt
    49621 tattgcagac aagttcaatc ggcgaacttt attgaaaata ggcggtagcg tgatgggact
    49681 ttctttcatc ctaccagctg ttttaggaac tgtactggat gctcatacaa attcgttgtt
    49741 gattctcttg tttttatgta tttatgtcgc attttattct tgcacatggg ctccactgac
    49801 atgggtaatc gtaggcgaaa tattcccact agctgtccgc gggcgagcat ctggcctagc
    49861 ctcttctttt aattggattg gttctttcct agttggcttg ctttttcctg tgatgactgc
    49921 ttctatgtct caagaaatcg ttttcggcat ttttgggatc atctgctttt taggggtttt
    49981 atttattcaa gagatcgtac cagaaactcg tgggaaatct ttagaggaaa tcgaacaaag
    50041 tgcatctaaa aaaacttatc ctaaacgtat ttctatccat cattcttaaa gaggttgtga
    50101 cagaagtggt cagcttcaag aaataagaag gaaccgctta gtttccagtc acaaggaaca
    50161 atttcaaaaa acagcttctc atgtttgatg aatattgtga cttgtgacca gttggtagga
    50221 gaacaccgtt tagtggagag gtggtgaaag aaacgttcag ccttgagcga tgacaaaagc
    50281 tctcacaaac attgcgacga gtaaccgtag gagcaataag aggcggtaaa aacccgtctc
    50341 ttatttttcg ttttctttca ctgtttcccg ttcaactagt acaggttcat agagaatatt
    50401 tggctgtgcc tgtccttttt caatcgttgt gacgatccac cgtgcggcat cttgtcccaa
    50461 cttttctttt ggatgcgtca atgttgtcaa ttttttattc cctaaatggc ttaaagaaga
    50521 atcgtctgtt cctacgatcg ataggtcatc tggaatatga tagccatttt ctgtcaactt
    50581 atctagcatt aaactagcta tttgatcgtt atagcaaatg atgccatcta aagcttgttc
    50641 ttctttcaac cgttgtactg ttttttcagc gattgtttct cgtgtttccg tggtgaaagt
    50701 aatgatatct tccgtcaaaa aacgcacatt atactgttca cacgcgctga taaaaccttt
    50761 catcctgtat tttccttgaa gatcgtcgat ttttgtgatg aacaacagac gctgatgtcc
    50821 tttttttatc aaatattctg ctgccataaa accgactttc acatcatcta aacaaatata
    50881 agaggcattt aactcttcat aagcagcatt gatcatcacc atcggaatgc cttgttctct
    50941 caaagcaaca taaagtgcta agtttgggtt atactgatta cttttcgtag gctcaacgat
    51001 caaaccatct accccataag caatcatttt cttcaaacac tctttctcca attcgtgatc
    51061 gttattcgta ctggctaaaa gcaatgaata cccagattct cgtaattcat tttcgattcc
    51121 tcggataatc gacggaaaaa tataatcaga gagatatgtg gtgatgacac cgatcgtttt
    51181 tgttctgttc aatggtgttt tttctttctt atagctgtca tctacatatg tgcccgaccc
    51241 tttttcttta cgcagatagc cctcatttac caacacagcg attgcttgcc gaatcgtatg
    51301 tctgctgacg tcatatattt cttgcagctt gaattctgag ggaatggttg tatttacttc
    51361 gtattcctca cgaaggatct tttgttttat ctcttctgca atatcttgat attttgccat
    51421 ttgccttgcc cctttttatt tgtacgtaca tatatcataa cgagtctgca aggtttattc
    51481 aagcaaaaat caaggtttct tgtgctcata aggcatacgg aacaggttca ccaaattctg
    51541 gaaatccttg ctcattccac gtaaagcatt gcgcgttcgc gtgccgatta ggattgtcta
    51601 acggatcccc tttttggttc ttctctggtc gagcatgata gatcaaaata tcaaagcacc
    51661 cgtcttctga aaccgtaaac gagttgtgac caggaccaaa ttgcttattt tttttagaag
    51721 aaacgaatac tggctccgat aatttatgcc atgattcttt taataaaaga ttcttgtgct
    51781 catcagccca cagcaggccc atggcatagt tttcatctgt tgcactacca gaatacgtaa
    51841 tgaatatttt cccattgcga gtaaggactg ctggaccttc atttactgaa aaaccccgtt
    51901 tttcccattc atactcaggt atagaaagga ggatctgctt cccctttaac gtccaaggat
    51961 tatccatttc ggatagataa agattagaat tcccagtaat ttcaggatct tgttgggccc
    52021 acacataata tagctttcct tgagaagaga aagttgttcc gtctaaactg aaagtttccc
    52081 ttttcgtttt tatttgtccc ttttctatcc aatctgtatt cattggatct atatgctgat
    52141 tttcaagtac gaacatgcgg tgatgatgag attcatcacg tatagtttta tgattgcttg
    52201 cagcaaagta aatataccat tttccattga tataatggac ttcaggtgcc cagatcaatt
    52261 cactcatcgg tccttcttca tgagcctgcc atacaatcag gcttgcagca tgttctaatt
    52321 cattcagtga ctttgccctt cttaattcaa tcacttggta gcctggaacc gatcctgtaa
    52381 aataataata accatctgta tgtttatata cccaaggatc tgcccgttgc cgaacgatag
    52441 gattattata aagtgtcttc atcataacaa atttcctcac attttcttta ttctaattac
    52501 attccaagag tatttttcta tccctacatt taattcgtca tgtttttcta ttgaaaaata
    52561 gtttatttca ggttttattt tttctggttc ttcacgtgta tttttgtcca acagatcgct
    52621 actggttaat gtgtagtgct ctccttgatc tagcaaattg aaatcatgtt caatcgttat
    52681 cttttgttgt tcatttgtgc gattgatcaa gaaaatagtc agctgattgc cgttatctac
    52741 gatcacactg tccacataag gaacattttt tatcttttca caatcataaa cagggcaaat
    52801 gatttgttgt ttcaatactt gacctttagc atatttcgat aagtgttgca tcacataata
    52861 agttggattc ctccatgctt gtcctttagc atctgtcagt attaaaggta ttgtgtttac
    52921 taacaatgat tgacaactaa tctttacgcg atctgcgtgt cgtaaaatag ccagccctaa
    52981 tgaaccaaat aataatgtat cttccatagt aaagtaacac caatcgatgg gactaccgac
    53041 ttcaaaatcc ctatgtcttt tttccggttg ggcatgtaca ttccattcat caaacgctag
    53101 atacattgtt ttctcacttc tcaaatatcc ttttacataa tcacaagtag caacgatttc
    53161 ttcaatttga cgatcgaatc gataagcttt tgcaaggtat gtcgctgtat catcacgctc
    53221 atttttagga ggtttagtca agtcttcttc atcatactta tcaatatagt tatgcaaagc
    53281 taaataatct acattctcat aagcttcttc taatgcgata cgatcccatt cagggtacga
    53341 tggtaaacgt ggcgtagagc ttccaactaa gattgtttca attgaatcat ccactaactt
    53401 catgactttt gaggcttcat ttgctaacct accatattca taagcactct tagcccccac
    53461 ttgccatgga ccgtctagct cattccctaa acaccataat ttaacgccaa aaggcttttt
    53521 ttgcccattt tttattctta aatcactcca gaaggttcct tttggaaaat tacaatattc
    53581 tactaaattt tttgcttcat caattccttt tgttcctaaa ttcaccgtga atatcggttt
    53641 cgcttgtaca tgactgatcc atttaaaaaa ttcgtggagt ccaaaagaat ttggttctat
    53701 acctcgccat gctaaatcaa ttttttttgg tcgttgttct ttcctaccta cagtatccac
    53761 ccaatcataa ccagatgtaa agtttcctcc aggatagcga atgacaccta attctaattc
    53821 ctttacataa gagaggacat cttgtctaaa cccgtcacca tcagctgttt catggcccgg
    53881 ttcataaatc cctgtataga cgacacttcc catatgctca acaaatgagc caaacagccg
    53941 agaactgatc tctcctaccg taaaatcctc tctgattttt actttcactt cttcgtccaa
    54001 cagctttccc tacctttcat ttcctatagt caagataata gatacaagtt tccatacgcc
    54061 accagcaaag ataaaaataa aaggagggaa aagaaatatg agcagtacaa atcctaataa
    54121 caatacgcaa caaagaatat ttctcaaaaa atcaaaaaat actttccatg cactttgttc
    54181 aaaaatttgt aaaaatgtga tatctttgtt ttcagaaatc acatatatcc aggataaaag
    54241 aaacatggaa taaataagta aaactacata tccactaatt gaaagtacga ttgtctgttc
    54301 ttctaataat tgattaacaa gaaaataaat aatgatcgtt aacagactaa ggaacaagtt
    54361 cttaccaaaa tgttgcttta ttccttgaaa gaaaaaataa atgatatttc ctgatccctc
    54421 acctttccat tgcgccatta cataaaaaac agcacatgta gcaggcacga tagtaataac
    54481 aggcaaacac acgattaccc agagaatacc taataagatg taatcagaaa ccacaatcaa
    54541 ctgtccataa agtttagtct caactatttt catcttcctc actctttcat tccagacata
    54601 gctgttcctt gaacaaaata tttctgtgca aaaatataaa ccactagtaa tggcagcatt
    54661 gaaataaccg ttccagccat tacaggtcca taaaaagaaa tgttctgccc tgtaaataaa
    54721 gctaaccctg ctggtaaagt tctcattttt gaactacttg tcaatatcaa tggataaagc
    54781 aagtcattcc aactattcat cagcgtgaaa atccctaatg ataaaagagt aggtttgctc
    54841 aatggcaaca tgattttcaa aaaaatatta aactcactta atccatcaat gcgtgctgcc
    54901 tcttccaaag aatctggtaa agtaataaaa aaggaacgca tcatgaagat accaaatgca
    54961 gttgtcatac gaggaatgat caaacctgca taagtatcaa gcatgccaag caaattgact
    55021 tcaataaata gaggaatcat gaagacttgg aatgggatca tcattgtcag caaaacgaaa
    55081 tagaacaaaa ctgattttcc tttgaaacgc atacgagcaa atgcataccc cgcaagagaa
    55141 tcaaagaata cagaagtgat cactgtccct ccagcaaaaa tcactgtatt tttgatgtaa
    55201 tctaataaag ggatcgttgt ccaaacctta atatagttat ctagggtata gctatctgca
    55261 aatattctag gcggaaatga gataatgtct ttttcgtgtt taaaagaaga aataatcagc
    55321 cacaaaaaag gaaacgcaat aaccaacaat ataactagca tactaatcgt tcgaatgatt
    55381 tttttaccac taactgcttt tcgactattc acagtaactg taatcgagct ttctggtgcc
    55441 atacaggttt ccctcctatt tcatttgttt ttctttccgt gacatgtaca cattagtgga
    55501 gatagctata gttgcaacaa tgataaacaa aaccgaagac agtgctgatg catagcccaa
    55561 ttcataaggt gctgtaaatc ctcgactgta aatatattga acagctgttt ctgtcttaaa
    55621 ttgtgggcca ccagcggttg caacatatac ttggtcaaac acttgcatcg aaccaatcag
    55681 attcgttaaa atacaaaaac ctaaggaggg aacgatctga ggtagcgtaa tgttaaaaaa
    55741 ctgctgtcga tttgttgcac catccatttg agcagattca tacaaagagg gagagattcc
    55801 ctgaatagca gtcaagagaa tcgtcatagt aacaccaaaa ttcttccaaa cagtcatgat
    55861 ggctaccgtc ggcatagcaa gggttggatc gcgaaaaaat tgagggatcg gcaagccgac
    55921 ttgtgaaaac aagtaaggaa tcgcccctac attcggatca agcaacatag aaaaaataat
    55981 cgcaattgac gtcaatgaac aaataactgg aatgtaaaat gtcgaacgac aaaaacgatt
    56041 gaatctggta tttttagata atgctactgc tgctaataaa ccaacgataa tttgtgttgg
    56101 cgtttcgagt acggtgaaat ataaagaatg aatcaacgaa ttaactgctc gttcatcact
    56161 aaatatacgt gtgaaatttt caaaacttac aaaatttctt gatgtaaaga aaatattcaa
    56221 atctgtaaaa ctaatagcaa atgttcctaa aagcggtatg acagtaaata ataataaaac
    56281 taaaaaagat ggagttaaaa acaaatacgc agcacgatca ggacgattcc aatatttggt
    56341 gaatccgttc atatagtcca cctcatcttt cattttgata aaatatacaa tcttggttta
    56401 aacgaatctg aataagatat ccgaacaatc gctgtgattt ttgcaataac acctatctgc
    56461 aaatactagg tcgatcattc agatatctgt atcttgccaa cagtctatcc tgtataaagt
    56521 tctgtactat ttttgcagta attgattgat ggcatcatct gcatcgttca tgagtgtctc
    56581 aggatcttct cctgctaata atttttcaat cagtggattg atgatatcgt tattgatcgc
    56641 agagatttgg gtaaaacctg gtaaatatgg ctctgcatag tccatttgtt tcgataattc
    56701 agatacaata ggatcatttt ttacttcttc atcttttgct actgaatcca agtaggcagg
    56761 aaaaccattt tcgatactcc attttttacc aatttcagtt gtattccaat atttcaaaaa
    56821 ctcatagaca gcttcttttt tattattatc tgtacttgcc ggaatggcat atcctacgcc
    56881 atctaaaata gctgtttttt tgtctgaatc actcccttgt ggtaaggtag tcacattata
    56941 atcaatctcg tttgctttca atccgttatt taaccatggc ccattgattt caatggctag
    57001 ttgaccagca ttcattaaat tgtccatttc ggcgccagaa atcgattctg gaccagtctt
    57061 gtctacatgg atcatttttt ggatcgcttt taacgtttta aggttctcag gagacgcaaa
    57121 ttcagactta gtataatcat cgttcactaa tttaccgcca tttgctaaca tccagttata
    57181 taacggtgca gttccatcct tattgaagac aaaacctgat acatttttac tactatcagt
    57241 taattttggt gccatttctg tcaattcttc ccaagtagta ggtggttgtt cagtatctaa
    57301 tccagcttgt ttaaacaaat cattattcca atacatatac atgccttgta cctgcatggg
    57361 gacaaaatac tgtttacctt ctacttcacc taattcaata gccgtttctt taaaatcaga
    57421 tttatctact ccatcatatt cccagaaatc gtctaatggt tgtaccgctc cgttttctac
    57481 atactgtgca aaatctggat aatttaatgc aataaagtca ggtgcttttt tagaagaaat
    57541 cgctgtagga agtttttcat tcaaattggc ccaagtcatg atatccattt caattttgat
    57601 gtttttatca tttgttttat taaattcatt aacgatttct tgcaatattt caccatcaga
    57661 tgcagtaaac ccattccaaa aagtcaaagt cacttcttta tcagaatcag attctgctga
    57721 acttccgcca ccgcaagctc ctaaaatacc tgttgcagct accaacaaag agccaattaa
    57781 ccatttttta cctttcattt tttgttcctc cttcatctga taaacgacat ctgaccacat
    57841 tccaagatag cggtttcaat aaacaattga ttttcccatc ttcagctttt acatttgttg
    57901 aatgacttgg ttttacttgt tcacttttag gaagattagt tgcttttgta tcaaatccac
    57961 tcatttcaga aaaatcaatc actccctcta atgataattc agacaagtga acagttagat
    58021 tcatttcttc ttctccacgg ttaactgcaa atatgacaag ttctttgtca tttattacgg
    58081 ctatactttc taaatacggc acttcttcaa aatcttccgt cgcatagttt gctgaacgaa
    58141 tttgtgggct taacacctct ccttgtccat acacagcagc ttgcataaat ggataataga
    58201 tcgtctgtct ccaagcttct ccgtttgttt ctgtcctgat tggtgcaata acatttacca
    58261 attgagcaag acaagcaatc tttactctat ctgcatgttt caataaagtg attaatagac
    58321 agcctaatag taatgcatct tcaaaattat agttatcttc tagtaaagga ggagcaatct
    58381 gccaaggcgc tgcttgctca tcttgttccg ctgaatgata ccaaatattc cattcatcaa
    58441 aggacaggtt gatctgcttc ttactatgtt tcttcgcctt gactgtatcg cacatcgctg
    58501 tcacttcaga aatgaaccga tccatgtcta aggaacgtgc aagataatga gaaagatcat
    58561 ttttttggtt gccataatat tgatgcagag acaaataatc tatttgatcg tacgcttgat
    58621 caagaactgt taactcccaa tcaccaaatg taggcatttg ccgattcgaa cttccgcata
    58681 aaaccacctc aattgaatca tctacgagtt tcatcgcctt agccgtttcc tctgctaacc
    58741 gtccatattc ttccgctgtt ttatgtccaa tctgccaagg accgtccatt tcattcccta
    58801 aacaccatgt cttaattcca aaagggctct cttgaccatt cgaacgcctt aaatcactcc
    58861 aataagttcc tttaggaaaa ttacaatatt cgactaaatt acgagctgca tctactccac
    58921 gagtacccag attgacagcc atattgattt cggcccccac atgctgacac cagttataaa
    58981 attcatgaat cccaacttca ttcgtttcaa ttgtccgcca agctaaatcc aaccttctag
    59041 gtctatttgc tatgggccca atactgtctt cccaacgaaa tcctgacaca aaatttccac
    59101 caggatatct aatcatggga atgttcaatt cttttaccaa gtgaatgacg tcttggcgaa
    59161 atccactttt attagctgat ggatgatctg gctgataaat cccttcatat actgctcgtc
    59221 ccatatgttc aataaatgaa ctgtacaacc gcggatcaac tttgcctaca cgcttgacac
    59281 tactaatcaa cattgtggct tccataactg acctccttta atttatacat gaataataca
    59341 cctcgttgaa agcgttgtca ataatataaa tgttaaactt acttattttt tatcgatttt
    59401 aattaaccaa aatattaata atgattaaaa caaataattt aatataactt ttattaacaa
    59461 atatgttaat atataagaaa aaccttttaa gaggtgatat atatgcaact acaaataaac
    59521 gaagaatcct taccggtata tgaagcatta gctagtaaaa caaggatcaa gatcatccag
    59581 ctactttcca aaaaaaagat gaatgtcaaa gatttggcaa aagaacttgg agtgagcagt
    59641 gctatcacga cgatgcacgt gaaaaagcta gaggaagcaa acatcatcaa aacagaaaaa
    59701 gtaggacaac aaaaaatttc gagccttcgt gtcgataaaa tcgatatcag ttttccagaa
    59761 aaaatattca atgcttttga tacgaaagaa acatcgatac ctattggtca ctatacaaat
    59821 tatgcaattg aaccaacatg cggtttagct acgatccatg attttatcgg gaaagtggat
    59881 gaaccacgct attttatgga tcctcgaagg atggatgccc gcattctctg gtttaccagc
    59941 ggatttgtcg agtatcaggc acctaatttt ttaaatacag aggatacatt ggagatgttg
    60001 gaagtttctg tggaaatttc ttctgaattt cctttttcaa atgacaattg gccttcagat
    60061 atcactttca gcctaaatgg tgtggagcta ggaacatgga cgagccctgg agattttgct
    60121 gatatccgcg gtaaatatac gcctgattgg tatccagata atcttaatca atacggcttg
    60181 ctgaagacga tccgaattac caaacacttg acgaatatga atggagaacc cttgtcaaac
    60241 atcacgatca acgacatccc taaagagcaa gacacttggc atttgcgaat cgaagtgaaa
    60301 gacgatgcca aacatgtggg gggctgtact ttatttggta aaggattcgg taattacgat
    60361 caggatatca aattaaaagt ttactacagc tgatattttt cttttctatt ttataaaaca
    60421 aaaacagcca atccctatga agggacggct gtttttgctt tattgttatt tccagaaatc
    60481 atccaaaacg gaaattggca aatgtcgttt atgttgtgtc ttgttccacc atgcttcgat
    60541 tttttcttgt gcttgagcgg gaacttcttt tccttctaga taatcatcaa tttcattata
    60601 tgtgacacct agtgcgactt catctgcaat cattggtttc ccatcttcaa gatctgcagt
    60661 cggaattttt gtgtataatt tttccggtgc attcaatgct tgaagcaatt gtttcccttg
    60721 tcgtttgtcc aaacggaata atggcaaaat gtcggctcca ccatcaccaa atttcgtgaa
    60781 gaatccagta atgttttctg cagcatgatc tgtccctaat acggctcctg ctctttctcc
    60841 agctacggca tattgcgtaa tcatacgttg acgtgctttg atatttcctt tgttaaaatc
    60901 gctgattccc acacctgctt cgcttaatac ttgtacttgt gcatcaacag ctggtttgat
    60961 atttactcgc agactaacat cgggctggat aaagtcaaga gcagcttttg catcttcttc
    61021 atctgcttgt tcaccataag gaagacgaac agcgataaat tgatagtttt catcttttgt
    61081 ttcttcacgc atttcttcca tcgttagttg cgccaaacgg cctgctaaag tagaatcttg
    61141 gccgccgctg atacctagta caaaagtttt aagaaacgga tattttttca aatatgcttt
    61201 cataaagtca atacttttac gtatttcttc ttttggatca attgttggtt tgacacctaa
    61261 ttcattgata atttctgtct gcaggctcat ttattctcct cctatgcttc tgtatgaagt
    61321 tctgctacgc tttttttcat tcgttccatg atagccattt tatggttcca gcattcagta
    61381 gacagatcga ctgggtattt ttgcggattc aagtctcgtt tatactcttc ccatagtgaa
    61441 tctagatttt cttttgtata ttctttgatt tcgtttagtg caggtaattc gtaaactctt
    61501 tttccttcga taaagatatc ttgtaaaact gggcgagctg taaagtctcg aacagtttta
    61561 ttgataaacg tatgcactgg atggaacatg aagatagctt cttcttctcg agggtcttca
    61621 tcccataatg tcacgtagtc tccttcagat ttcccatcaa aatttcgagt gatgcgccac
    61681 acttgttttt tccctggagt agttactttt tctgcattgc ttgataactt gatcgtatcc
    61741 atcatttttc cttcatcatc ttcgatcgat actagcttga atactgcacc aagggctggc
    61801 tgatcatagg ctgtgatcaa ttttgtccca acaccccata catcaatttt agctttttgc
    61861 atctttaaat tcaaaatcgt tgcttcatcc aagtcattcg aagcatagat ttttgcttcc
    61921 gtaaatccgg ctgcatctaa ttgttcccga acacgcttag atatataggc catatctcca
    61981 ctgtcgatgc gtacgccttg gaagttgatt ttgtcaccta attctttcgc cacgcgaatc
    62041 gcacttggca cacctgattt caacgtatca taagtgtcga ctaagaacac gcagtcttta
    62101 tgggttttag cgtaggccat aaatgcttca taatcatttc cataagattg gactaacgaa
    62161 tgagcatggg tcccgctagc tggtatccca aatatcttcc cagcacgtac attgcttgtt
    62221 gcatctgctc cgccgatata tgccgctcta gttccccaga ctgctgcatc cagttcttgt
    62281 gcccgtctag ttccaaattc caatagtgga tcttctccga tgacagattt gatacgagct
    62341 gcttttgtcg cgatcaacgt ttggaaattg accatattca acagtgctgt ttctactaat
    62401 tggcagtgcg ctaaaggccc ttccacttgg attaatggtt catttgcaaa aactagttct
    62461 ccttcacgag cagaacgaac tgttgcttta aattcaaaat tttgtaaata ctccaaaaaa
    62521 ccttcaggat agacttctaa tccacgcaag taagcaatat cggaatcatt gaacgtcaga
    62581 ttctctaaat aattgactaa ccgttccaat cctgcaaaaa ccgcgtatcc gtggttaaaa
    62641 ggcatttctc gaaagtaaca ttcaaaaaca gcatgtaaat cagctcgacc taattcccag
    62701 tatgtctgca tcatattgat ttgatataga tccgtatgca gcgtcaaact gtcgtctggg
    62761 taagtattat tcatcttgta ttactcctct tggcaagttt tcatttccta cccattataa
    62821 caaaaaaaat taataggaaa agaaatcttc cattttttct gaaaaagcac atgttttacg
    62881 gatatttctt gctaaaaatg aactccatct gctaaaacta tcctttctta ttggtattta
    62941 acctaaacga gaaagaatac ttattttatt caatgtatcc gagacacgaa gaaattagcg
    63001 ttttctattt ttcttacttg ataaaataag tagattttcc tactacaatc gtagccgtta
    63061 agataccgat gattgaacaa atataaccaa atgtctctac aacataatca atggcaaaag
    63121 tttttgaagc aataatacgt ctttcctttt ttgcttctta tccccaaaga aacggatata
    63181 acttattatg tattgaattt ttttggaaag gcgaattatg acaagatata ccttgcgttg
    63241 aaaaaactcg cggaaaaaat atcacacgtt tttaaaaaga tacaaagtaa gaaagcagct
    63301 gtcaatagaa tggaaacaaa aatggcgaga catcaatcag tctcgccttg ttttttattg
    63361 tatacattag atatggagca ggttaccata aagatgatta ttagtcgtta ttaaatagaa
    63421 tccttcgtat ttttttgttc tatcactaca atatctttat ttggacgttt gaattgtatt
    63481 ccggcacgtt ctagctcctg tttcactcct acatggactt tgacatcttc tcgtgtcatt
    63541 tttaaaacgt gatgaatcgc atagtcgatt tcttgccttg tgattttttt cttcgtattg
    63601 atcatcacga cttgcttttt atcatatgtg tctaccaata gcagttgtgt tcgatctaac
    63661 gaaaataatt tgtaataatt aatagccgta ttcccaaaaa agaattccac tactttagga
    63721 taaaatgtct taatatttaa attactattc acaggtgttt cgattagctt catctcattc
    63781 ctcttttcta tatagtcgat ttagatccaa tcttaagtta tgttaccatc tttcctagca
    63841 ttaaggaatc ggttacaatt gaacctttaa agacaaaagg atttaccttt tatcacttaa
    63901 tagtataaca taatttcgtg agaaaaaaat acaatttttt cttgaccatc tcgttacgta
    63961 aaggattatg cttacatttg tcaggaggaa aaataatgga gtacacaata aagaaaatgt
    64021 cagagatatc aggagtcagt ccgagaacct tgcgctttta tgatgagatt ggactgctaa
    64081 aaccagctag gatcaattct tcaggttatc ggatttatgg caaaaaagaa gtcgatcgtc
    64141 tgcaacacat cttattttat cgaacaatgg cattcaagtt ggatgatatc caagaggtct
    64201 tagacaatcc atcttttgac caccagaaag ctttaatcaa gcatcagcaa atgttgctgg
    64261 aaaaacgggc acaaatcgat accctcttga caactgtcca acagacactt gatatgtacg
    64321 aaggaggaag aaaaatgagt gacaaagaaa aatttgaagg gtttaaacaa caaaagttga
    64381 aagaaaatga agaatcctat ggacaagaga ttcgacaaaa atacggaaaa aaaaaggtta
    64441 cagcatccaa tgaaaaatgg atgaatatgg ataaagaaac gtatgaagaa atgcaaactg
    64501 ttgaaaagca gatgttattt gatctcactc tttacttaaa agaaccagaa gatgaaagct
    64561 tagcagaccg tatcttccaa gctcataaga agtggttctc ttacagctgg cctgaatatc
    64621 agccaaaagc gcataaagga ctaggaatga tgtacgaagc cgatgaacgg ttcactgcat
    64681 attatgacga gcgaagtggt catggtgcag caaaagcttt aaatgaaatt atccaaaaaa
    64741 aggcgtaatc ttttacatga tccaacaaaa aagtacacgt aaaatgacct aataacaaaa
    64801 cagcagacac ccaagttaga tttttatgct aacttttggt gtctgctcca ttctttcaca
    64861 agttttttca tcctacttta aatatattca tcgttgaata aacgtataag gtattttcac
    64921 tttatccttt ttttctgtaa gaaacaattg aaatgccatt tctccgcaag cttccagatg
    64981 gtggtcgatg gttgggaaac caagaagctc gctgatcagc aaattatcgc gtccaatcac
    65041 cactggtgtc ttatcttgca gatagctttg caagatgcct gctgcaacca tatccgaatt
    65101 tgtaaagaca ccatcaactc caagctcttt aaaaaagaag cctgcttgtt ttcctttctc
    65161 tgcatctata caatcccaaa aaacaagtga ttcatcaaaa gatggaaaaa gttgatgaca
    65221 tagctgcaaa gtgatttttg agttataact tagcctgcca cttcgcccca acgtgatgcc
    65281 caattgcttt ataccttgct gcttcaaata agtaagacct tctgtaattg attgttctcg
    65341 atcaataaag acacagcttg cctgttttcc cttgatttct tcacaaaaaa ctagaggtcc
    65401 atatttttga tatgccaata aatcatctag tgtattggca cgagacgtaa tgatcaatcc
    65461 atcgaaagct ttagctgcaa attcttcaag ataccttcgc tctttttctt tatgatagcc
    65521 cgtcggtaaa agtgtcactt tgtaatcata atcaaacgct gcttcaatga tcccactgat
    65581 caactgatca aaatacggat gattggtata aggcagaatc actccaatgc ttttcgtttt
    65641 tccataactt aactgtctgg catttgcatt agggatataa tcgacttctt tcatgaccgc
    65701 aagtatttct ttcctttttt cctcatctac atatggatgt ccattgatta cacgtgaaat
    65761 agtagaaact gaataacctg tgagctttgc gatatctcga atgtttgcca ctgctgcttc
    65821 gccacctata caaaaagatt ttcttgtaaa tagtctacca cagctggacc gcattcaaaa
    65881 aactgataca tttttccttc atgcgaaaaa ctgatccgct gcccgcccca atatatatct
    65941 tttgtcaaat tttcgatttc attcattggt atttctttta ctatattcct attcaaccct
    66001 tgaaaactgt acaacgttaa tacggatccc ttgaactcga caaacgcata aaaataattg
    66061 ttcgttgtgc catctgttat ctctacataa gtattcattg caagccctcc ttttgatact
    66121 ttgagtatag tgtatgaaac gcgtctcata gcaaggagtt tgttttgcca aagaaaagtt
    66181 ttattcctct ttcattatta ccaaagtctc ttattttaaa ttgaatacat tataatgata
    66241 ctgtaaatat atctttatta ttagtgaatg
//
LOCUS       DANJMI010000010        65939 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000010 DANJMI010000000
VERSION     DANJMI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.010"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            624..1487
                     /locus_tag="PWU81_000778"
     CDS             624..1487
                     /locus_tag="PWU81_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL1109506.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1616..3238)
                     /locus_tag="PWU81_000779"
     CDS             complement(1616..3238)
                     /locus_tag="PWU81_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1109507.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3487..4008
                     /locus_tag="PWU81_000780"
     CDS             3487..4008
                     /locus_tag="PWU81_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL1109508.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4194..5924)
                     /gene="spxB"
                     /locus_tag="PWU81_000781"
     CDS             complement(4194..5924)
                     /gene="spxB"
                     /locus_tag="PWU81_000781"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDL1109509.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            6089..6907
                     /locus_tag="PWU81_000782"
     CDS             6089..6907
                     /locus_tag="PWU81_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL1109510.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(7010..7207)
                     /locus_tag="PWU81_000783"
     CDS             complement(7010..7207)
                     /locus_tag="PWU81_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109511.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7376..7954
                     /locus_tag="PWU81_000784"
     CDS             7376..7954
                     /locus_tag="PWU81_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL1109512.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            8049..8186
                     /locus_tag="PWU81_000785"
     CDS             8049..8186
                     /locus_tag="PWU81_000785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109513.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8999..9502)
                     /locus_tag="PWU81_000786"
     CDS             complement(8999..9502)
                     /locus_tag="PWU81_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL1109514.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9861..10886
                     /locus_tag="PWU81_000787"
     CDS             9861..10886
                     /locus_tag="PWU81_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL1109515.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11209..12087
                     /locus_tag="PWU81_000788"
                     /pseudo
     CDS             11209..12087
                     /locus_tag="PWU81_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
     gene            12563..12679
                     /locus_tag="PWU81_000789"
     CDS             12563..12679
                     /locus_tag="PWU81_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL1109516.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13607..13945
                     /locus_tag="PWU81_000790"
     CDS             13607..13945
                     /locus_tag="PWU81_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109517.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            14039..14269
                     /locus_tag="PWU81_000791"
     CDS             14039..14269
                     /locus_tag="PWU81_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109518.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14669..15040
                     /locus_tag="PWU81_000792"
     CDS             14669..15040
                     /locus_tag="PWU81_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL1109519.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15314..16006)
                     /locus_tag="PWU81_000793"
     CDS             complement(15314..16006)
                     /locus_tag="PWU81_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL1109520.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(16009..16794)
                     /locus_tag="PWU81_000794"
     CDS             complement(16009..16794)
                     /locus_tag="PWU81_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL1109521.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            17031..18431
                     /gene="lacG"
                     /locus_tag="PWU81_000795"
     CDS             17031..18431
                     /gene="lacG"
                     /locus_tag="PWU81_000795"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDL1109522.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18456..18773
                     /locus_tag="PWU81_000796"
     CDS             18456..18773
                     /locus_tag="PWU81_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL1109523.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18786..20459
                     /locus_tag="PWU81_000797"
     CDS             18786..20459
                     /locus_tag="PWU81_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HDL1109524.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20473..22104
                     /locus_tag="PWU81_000798"
     CDS             20473..22104
                     /locus_tag="PWU81_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HDL1109525.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22595..24160)
                     /gene="guaA"
                     /locus_tag="PWU81_000799"
     CDS             complement(22595..24160)
                     /gene="guaA"
                     /locus_tag="PWU81_000799"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL1109526.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(24219..25142)
                     /locus_tag="PWU81_000800"
     CDS             complement(24219..25142)
                     /locus_tag="PWU81_000800"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL1109527.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            25338..25943
                     /locus_tag="PWU81_000801"
     CDS             25338..25943
                     /locus_tag="PWU81_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL1109528.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            26251..27267
                     /locus_tag="PWU81_000802"
     CDS             26251..27267
                     /locus_tag="PWU81_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL1109529.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            27408..28709
                     /locus_tag="PWU81_000803"
     CDS             27408..28709
                     /locus_tag="PWU81_000803"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDL1109530.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28755..29417
                     /gene="deoC"
                     /locus_tag="PWU81_000804"
     CDS             28755..29417
                     /gene="deoC"
                     /locus_tag="PWU81_000804"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL1109531.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            29438..29836
                     /locus_tag="PWU81_000805"
     CDS             29438..29836
                     /locus_tag="PWU81_000805"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL1109532.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29902..30984
                     /locus_tag="PWU81_000806"
     CDS             29902..30984
                     /locus_tag="PWU81_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1109533.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            31196..32758
                     /locus_tag="PWU81_000807"
     CDS             31196..32758
                     /locus_tag="PWU81_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1109534.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32751..33884
                     /locus_tag="PWU81_000808"
     CDS             32751..33884
                     /locus_tag="PWU81_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1109535.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33884..34840
                     /locus_tag="PWU81_000809"
     CDS             33884..34840
                     /locus_tag="PWU81_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1109536.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34939..36105
                     /gene="deoB"
                     /locus_tag="PWU81_000810"
     CDS             34939..36105
                     /gene="deoB"
                     /locus_tag="PWU81_000810"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDL1109537.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            36129..36947
                     /locus_tag="PWU81_000811"
     CDS             36129..36947
                     /locus_tag="PWU81_000811"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL1109538.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36964..37671
                     /gene="deoD"
                     /locus_tag="PWU81_000812"
     CDS             36964..37671
                     /gene="deoD"
                     /locus_tag="PWU81_000812"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL1109539.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(37969..38655)
                     /gene="gpmA"
                     /locus_tag="PWU81_000813"
     CDS             complement(37969..38655)
                     /gene="gpmA"
                     /locus_tag="PWU81_000813"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL1109540.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(38827..39408)
                     /locus_tag="PWU81_000814"
     CDS             complement(38827..39408)
                     /locus_tag="PWU81_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1109541.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(39541..40224)
                     /gene="rpiA"
                     /locus_tag="PWU81_000815"
     CDS             complement(39541..40224)
                     /gene="rpiA"
                     /locus_tag="PWU81_000815"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL1109542.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            40543..40956
                     /gene="rpsL"
                     /locus_tag="PWU81_000816"
     CDS             40543..40956
                     /gene="rpsL"
                     /locus_tag="PWU81_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL1109543.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            41040..41510
                     /gene="rpsG"
                     /locus_tag="PWU81_000817"
     CDS             41040..41510
                     /gene="rpsG"
                     /locus_tag="PWU81_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL1109544.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            41604..43691
                     /gene="fusA"
                     /locus_tag="PWU81_000818"
     CDS             41604..43691
                     /gene="fusA"
                     /locus_tag="PWU81_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL1109545.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            43863..45050
                     /gene="tuf"
                     /locus_tag="PWU81_000819"
     CDS             43863..45050
                     /gene="tuf"
                     /locus_tag="PWU81_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL1109546.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            45457..45765
                     /gene="rpsJ"
                     /locus_tag="PWU81_000820"
     CDS             45457..45765
                     /gene="rpsJ"
                     /locus_tag="PWU81_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL1109547.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            45796..46425
                     /gene="rplC"
                     /locus_tag="PWU81_000821"
     CDS             45796..46425
                     /gene="rplC"
                     /locus_tag="PWU81_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL1109548.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            46453..47076
                     /gene="rplD"
                     /locus_tag="PWU81_000822"
     CDS             46453..47076
                     /gene="rplD"
                     /locus_tag="PWU81_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL1109549.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            47076..47366
                     /gene="rplW"
                     /locus_tag="PWU81_000823"
     CDS             47076..47366
                     /gene="rplW"
                     /locus_tag="PWU81_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL1109550.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            47405..48238
                     /gene="rplB"
                     /locus_tag="PWU81_000824"
     CDS             47405..48238
                     /gene="rplB"
                     /locus_tag="PWU81_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL1109551.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            48281..48559
                     /gene="rpsS"
                     /locus_tag="PWU81_000825"
     CDS             48281..48559
                     /gene="rpsS"
                     /locus_tag="PWU81_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL1109552.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            48582..48929
                     /gene="rplV"
                     /locus_tag="PWU81_000826"
     CDS             48582..48929
                     /gene="rplV"
                     /locus_tag="PWU81_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL1109553.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            48943..49599
                     /gene="rpsC"
                     /locus_tag="PWU81_000827"
     CDS             48943..49599
                     /gene="rpsC"
                     /locus_tag="PWU81_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL1109554.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            49602..50036
                     /gene="rplP"
                     /locus_tag="PWU81_000828"
     CDS             49602..50036
                     /gene="rplP"
                     /locus_tag="PWU81_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL1109555.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            50026..50214
                     /gene="rpmC"
                     /locus_tag="PWU81_000829"
     CDS             50026..50214
                     /gene="rpmC"
                     /locus_tag="PWU81_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL1109556.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            50239..50505
                     /gene="rpsQ"
                     /locus_tag="PWU81_000830"
     CDS             50239..50505
                     /gene="rpsQ"
                     /locus_tag="PWU81_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL1109557.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            50563..50931
                     /gene="rplN"
                     /locus_tag="PWU81_000831"
     CDS             50563..50931
                     /gene="rplN"
                     /locus_tag="PWU81_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL1109558.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            50968..51276
                     /gene="rplX"
                     /locus_tag="PWU81_000832"
     CDS             50968..51276
                     /gene="rplX"
                     /locus_tag="PWU81_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL1109559.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            51304..51843
                     /gene="rplE"
                     /locus_tag="PWU81_000833"
     CDS             51304..51843
                     /gene="rplE"
                     /locus_tag="PWU81_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL1109560.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            51862..52047
                     /locus_tag="PWU81_000834"
     CDS             51862..52047
                     /locus_tag="PWU81_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDL1109561.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            52083..52481
                     /gene="rpsH"
                     /locus_tag="PWU81_000835"
     CDS             52083..52481
                     /gene="rpsH"
                     /locus_tag="PWU81_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL1109562.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            52513..53049
                     /gene="rplF"
                     /locus_tag="PWU81_000836"
     CDS             52513..53049
                     /gene="rplF"
                     /locus_tag="PWU81_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL1109563.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            53210..53566
                     /gene="rplR"
                     /locus_tag="PWU81_000837"
     CDS             53210..53566
                     /gene="rplR"
                     /locus_tag="PWU81_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL1109564.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            53587..54087
                     /gene="rpsE"
                     /locus_tag="PWU81_000838"
     CDS             53587..54087
                     /gene="rpsE"
                     /locus_tag="PWU81_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL1109565.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            54102..54281
                     /gene="rpmD"
                     /locus_tag="PWU81_000839"
     CDS             54102..54281
                     /gene="rpmD"
                     /locus_tag="PWU81_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL1109566.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            54325..54765
                     /gene="rplO"
                     /locus_tag="PWU81_000840"
     CDS             54325..54765
                     /gene="rplO"
                     /locus_tag="PWU81_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL1109567.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            54765..56060
                     /gene="secY"
                     /locus_tag="PWU81_000841"
     CDS             54765..56060
                     /gene="secY"
                     /locus_tag="PWU81_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL1109568.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            56122..56769
                     /locus_tag="PWU81_000842"
     CDS             56122..56769
                     /locus_tag="PWU81_000842"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL1109569.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            56960..57178
                     /gene="infA"
                     /locus_tag="PWU81_000843"
     CDS             56960..57178
                     /gene="infA"
                     /locus_tag="PWU81_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL1109570.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            57211..57327
                     /gene="rpmJ"
                     /locus_tag="PWU81_000844"
     CDS             57211..57327
                     /gene="rpmJ"
                     /locus_tag="PWU81_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL1109571.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            57345..57710
                     /gene="rpsM"
                     /locus_tag="PWU81_000845"
     CDS             57345..57710
                     /gene="rpsM"
                     /locus_tag="PWU81_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL1109572.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            57738..58127
                     /gene="rpsK"
                     /locus_tag="PWU81_000846"
     CDS             57738..58127
                     /gene="rpsK"
                     /locus_tag="PWU81_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL1109573.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            58207..59145
                     /locus_tag="PWU81_000847"
     CDS             58207..59145
                     /locus_tag="PWU81_000847"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL1109574.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            59173..59553
                     /gene="rplQ"
                     /locus_tag="PWU81_000848"
     CDS             59173..59553
                     /gene="rplQ"
                     /locus_tag="PWU81_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL1109575.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            59825..60664
                     /locus_tag="PWU81_000849"
     CDS             59825..60664
                     /locus_tag="PWU81_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL1109576.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            60640..61509
                     /locus_tag="PWU81_000850"
     CDS             60640..61509
                     /locus_tag="PWU81_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL1109577.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            61499..62296
                     /locus_tag="PWU81_000851"
     CDS             61499..62296
                     /locus_tag="PWU81_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL1109578.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            62499..63245
                     /gene="truA"
                     /locus_tag="PWU81_000852"
     CDS             62499..63245
                     /gene="truA"
                     /locus_tag="PWU81_000852"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL1109579.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            63910..64147
                     /locus_tag="PWU81_000853"
                     /pseudo
     CDS             63910..64147
                     /locus_tag="PWU81_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(64291..65067)
                     /locus_tag="PWU81_000854"
     CDS             complement(64291..65067)
                     /locus_tag="PWU81_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL1109580.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            65190..65597
                     /locus_tag="PWU81_000855"
     CDS             65190..65597
                     /locus_tag="PWU81_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDL1109581.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT        20962 a        11760 c        13884 g        19333 t
ORIGIN      
        1 aaaccgtgtc acagtccctt tttccgaaca aacggtgttc aaaagcttgc atctacggca
       61 ctaagcccaa tcagatgaaa aatatggagt gctattttta tctgattgtt cttactgctt
      121 gatgcagagc agctttttca caacctctcc actaaacggt gttctcctgc caactcctcg
      181 gtcacaagtc acaatattca acaaacatga gaagctgttt tttgaaattg ttccttgtga
      241 ctcggagcta aacggcaggc tcacaacctc ttggtaaacg gtgttcaatc agctgaccct
      301 cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttttccgttt ttttctctaa
      361 tactcaggtc aaaacgctga tttcacaacc tctgattcac ggtgttccag aagtagcttc
      421 tacggaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa attccttctt
      481 atttcttgaa gctgaccact tctgtcacaa cctcttattt tgattagaac ttgcgaaaat
      541 aaaccggtta aagtgtgtct agcgcaagag atttgctata atagacgata agggtaaaat
      601 gtaacgagaa ggggttgaaa aatgtgtatc aaattgtaac agattcttgt tgtgatctgc
      661 cataccatgt attagcaaaa gaaaaagttg attttttatc aatgaatctc caattgaatg
      721 atcaagagtt agtagatgac ctcggaaaga cattcaatta tgaccgctac atgaatcagc
      781 tgaaaaatgg tgcgatgcct acgacatcac aaatcaatgt tggccgatat ttagaatttt
      841 tccgttctta tgcggaaaaa cagattcctg ttttatatgt ggctttttcc agtggattaa
      901 gcggctctta cagtagtgcg cttcaagcgg ttgaattgct gaaggaagaa tatgaagacc
      961 ctgagatcta tgtttttgat acaaaagctg caagtattgg ggaagggctt ttagtattgg
     1021 aagctgtaag attgaaaaaa gaaggtaaaa gcatcgaaga aaccctagag tggctggaaa
     1081 gtaattattt gaaagtccat tcatgggtaa cagtagacga tctgaaatat ctagaacgag
     1141 gcggtaggat ttccaaagca agtgctactc ttggcagtct attgagtgta aagcctatta
     1201 ttacggtgga taaagaagga aggctgacga atgttgacaa agttcgagga agaaacaagt
     1261 cgatccagac aatcgtcaat gagacggtaa aaacaatcgt tgcaccactt gatcaaatcg
     1321 tttatgtggc ttacgctggg gatctagaga gcgcggagaa ggcaaaacgg cttttggaag
     1381 agcagataaa gatgaaagat gtgaagcttt atcccttagg accaacgatc gccagtcaca
     1441 caggctatgg ctgtatcgcg attttttcga tgggaatcag tcgataaaag cagtaactca
     1501 ttctgtaaaa aagcccagaa attgattgta acaaaaacag cgactcttaa aagatagatg
     1561 agcatcctat ctcttagagg tcgctgtttt tttatttttt aagctaaata ggccattatg
     1621 cccataactc gttcaattgt ttttgaatat ctgcattttc caaaaattca tcatatgtcg
     1681 tatcagctcg atcgatcaca cctttatccg aaacggcaat gatgcggttt gccaatgttt
     1741 ggataaattg atggtcatgt gacgcaaata agattgagcc agtaaatgcc atcaagccgt
     1801 cattcaatgc tgtgattgat tctaagtcta aatggttcgt tggatcatct aatacaagta
     1861 cgttagcttt cgaaagcatc atttttgaaa gcatgcaacg gactttctct ccaccagata
     1921 aaacattcac tggtttcaat acttcttcac cagaaaacaa cattcttcct aagaagcttc
     1981 ttaagaaggt gttatcatct tcttctttac ttgcatattg acgcaaccag tctaaaatcg
     2041 ttagatcagt atcgaattct ttcgttgtat cctttggtaa ataagaatta cttgttgtca
     2101 caccccaacg tactgttcca gtatctggcg taatttcacc catgataact ttgaatagcg
     2161 ttgttgttgt tatatcattt tctgcgacga aagcgacctt gtcttctttg ctcaaattaa
     2221 aagatatatc atctaaaatt ttctttccat caatcgttac acttacattt tctacctgca
     2281 ataagtcgtt tccaatctca cgttcaggtt tgaatccaac gaatggataa cgtctagaag
     2341 aaggttggat atcgtctaaa gtaatttttt caagcatttt tttacgtgaa gttgcttgtt
     2401 ttgattttga tgcattcgca ctaaagcggg caataaattc ttggagttct ttgatttgtt
     2461 cttctttttt ggcatttgcg tttgcttgaa gtttagccgc tagttgacta gattccaacc
     2521 aaaaatcata atttcctaca taaagtttga ttttaccaaa atctagatcc gccatatgtg
     2581 tacatacttt gttcaaaaag tgacggtcat gggatacaac gatcacagta ttttcaaaat
     2641 tgatcaaaaa ttcttccagc caattgatcg attggatatc cagaccgttt gttggttcgt
     2701 ctagcaaaag aacatcaggt ttaccaaata gtgtttgtgc taataacact tttacctttt
     2761 gtccaccagt taattcactc atttgctgat ggtgcaattc ttcagggatg ttcagacctt
     2821 gcagtaaaac agctgcttct ggttcagctt cccatccatt aagctcagca aattctcctt
     2881 ctaattcagc tgctcgaata ccatcttcgt cagtgaaatc ctctttcatg taaatcgcat
     2941 ctttttcttt cagcacttca tacagacgct tgtgtcccat gatgactgtt tcaagtacag
     3001 tatagtcttc atagtcgtaa tggttctgtt tcaatacggc catccgttca tctggtccca
     3061 tagtaacgga acctgtggaa ggctgcagat cccctgataa cactttaagg aacgttgatt
     3121 tacctgcacc atttgcacca atcaagccat agcagtttcc gggggtaaat ttaatattca
     3181 catcatcaaa tagtttgcgg tctgaaaagt gcaaactcac atcatttaca gtaatcaatt
     3241 tttttcctca cttttatcat tattagtttt gacaagtctt atagaattat ataggggaaa
     3301 ggtaaaagat tcaaggcaat cgaacattct ttgtgtttca cgtgaaacag aatgaaaaaa
     3361 ataaaaataa aagcggagcg aaaatagcta ctataggatt tattagtttg caacagcata
     3421 atttttgacg ttatttataa gagggagtac tataaaactg taaaagaaat gaaagaagga
     3481 ttgaaaatgg atgaaaaaag aaccatcgac tggggctcat tgattttggg aattttattt
     3541 gtacttgtct cattgttatc atttcaagac cctgtcggaa atttagttgc aattgtagta
     3601 gtctttgcga tttttgcctt tattaaaggg atatttgaac tgtttgtacg gaacagatta
     3661 aaagagctaa caggatataa aggaaagatg cccttaataa tcgggatcat cgatattcta
     3721 gttggcgtct ttttcttatt caatattggt gcaggagtcg cagcattgcc ttttgttttt
     3781 gcagtatggt ttattgctga ttcagtttta gcgttattta ctgctgactt ggcaagagga
     3841 gtaagtgaag gatattattg gtttacgatc atcgtcaata ttttaggaat tttattagga
     3901 attttcttat tgtttaatcc aatttcttct gcgttgacat taagcttttt agttggattc
     3961 tattttatgt tatttggtat cacacatatc gtctatgcat ttagataagt aaaatcaaac
     4021 aaataaaaaa gccggcttcc gattacagtt ttactgttgt aattggaagc cggctttttt
     4081 atcatggaca aatatgcacg aggtgatttt taggtgtcga gacaaattta tgcaaaaatg
     4141 tgagaagcta tatacacaaa ttttatctta atactcgaag catagcgcag ttgtcacaac
     4201 ctcttttcat ataattcatt caatactggc aaatcatgga cttcatattt ttctttgaat
     4261 gcttggattt cttcttcaga atattttctt ggatctaaat gcagatcttc tactggcaat
     4321 gggcgcttgt tattgatctt cacatcgatt actacaggac gatctgattt tgcagctgca
     4381 tcaaaggcaa atcgaagttg cgagtattct gtgactgtaa agctatttgc cccgagcgct
     4441 tctccaactt taccgaaatc tgcatcttct aaaaatacac caaatttctt ttgttccgta
     4501 tcttcttgtt ctgcttcgat gaaaccaaaa gaatcattcg aaaagacaac attaataatt
     4561 ggaagctgat atttgacttg tgtaatgata tcttgcatga ccatagcaaa cccgccatca
     4621 ccacttaagg taaatacttg tttttctggg tagcttagtt gtgcggcaat tcctcctggc
     4681 acaccattac ccatagttgc gaaccatcca gatgtcgtat gtttttgttt accgttcata
     4741 ttcaataaac gaatagaatg gatagtcgtg ttaccgacat ctgtgacgaa aatcgcatcg
     4801 tcttcagcaa tacgattgat ttctttatag acagcctctg gacgaatcgg ttcttcctct
     4861 cgatcttcaa aagatttcaa ccaatttacc catttttctt tattttcttg atttgctagg
     4921 taccagctat cagcagggcg agcatcccct aactcgacaa gtctttgcaa agctgtgttt
     4981 gcatcaccga ggatcgacaa agatgtttca tggcgtcgtc caaattttga cgcatcgata
     5041 tcgatttgga taaattcagc agttggatta aaaaatgcac gtccaaatgg gaagtcgcta
     5101 ccgacaaata agatcagatc ggcttctgcc aatgcttcat ttccgggttt agtagcgaca
     5161 cgtccagcaa aaccaagaaa gttttcataa tcatcaggaa taattccttt tgctagaacg
     5221 gaagcgacaa caggaactga aaaatgttct gagaattctt tgatttgcgg aaatccgttt
     5281 cgcgttcctt gaccaacata aagaactggt ttttttgctt tttcaagata aggcaaagca
     5341 gctagcaaat ctttttcttc tggtaatatc acacttgttt tgtgagtgtg agcggtggag
     5401 aaaggttgtt cgtcaatttc ttcaaaacca agatcaacag ggatagtcac gatagccacg
     5461 cctttatgct cataggcggc tttaatggct tcatctacta catgcggaag actttgtggt
     5521 gtcatgactg tacgattgta tacgctaaca tctgcgaaaa taggattttc atttagttcc
     5581 tggaaagagt tatagttcat ggatgtagaa gcaacttgtc ctaagaacgc tactagaggc
     5641 acatggtcca tctgtgcatc atacaaacca ttgatcaaat gtgtcgcacc aggtcctgca
     5701 gagccgaaga cagcgccgac tttccccgtt aattttgcat ctgcagcggc tgcgagtgcg
     5761 ccgacttctt catgacgaac ttgaatgtag ttgatttcgg ttttttcttg atatagcgca
     5821 tccataattg aattgaaaga tcctccggga atgccgtaaa tatggtcgat accccagctt
     5881 tcaagaactt ttaccattgc gattcctgca ttgatttttg acatggtgtt tcactccttt
     5941 agcaatgatg atacataaat tttttgtatc tagagtatag aactttttat cgaaaatccc
     6001 aactaattcg tcttttcaca aaaatgtagt catgtttttg ggaatatgct agagtagtac
     6061 atagatagat tcaagagagg ggaatggcat ggttgaatgg ctattgagac ttgaaccttg
     6121 gcagcaagca cttgctggga cagcttttac ttattttatg acagctcttg gtgccgggtt
     6181 ggtttttttc tttaaagaaa tcaaaaaaga agtgctaaac ttgatgttag gatttgcttc
     6241 tggtgtcatg attgctgcaa gtttttggtc tttgttagat ccggctatta caaaagcaga
     6301 agaaaatgga gatattgctt ggctagttgt aagtattggg tttggtttag gcggtgtgtt
     6361 tttatatatg gcagataaaa cactgcccca tatgcatttt ggaccacaac atgaagcaga
     6421 aggattgcct acccatttaa aacggacgat cttgcttgtt ttttctatta cattgcataa
     6481 cattcctgaa ggtttagccg tgggggtagc attcggtgca gcagcaactg cggataatcc
     6541 gacagccgct atattagccg ctgtttcggt ggcgctaggt attggtatcc aaaacttccc
     6601 agaaggagca gctgtatcaa tcccgttgcg acaagaaggt ctgagtcgta aaaaggcctt
     6661 tgtttacgga caagcttctg gaatcgttga accaattgct ggagtgattg gagcgctact
     6721 agtaacgaaa gtggagctgc tgcttcctta tgcacttgct tttgccgcag gagcaatgat
     6781 ttatgtcgtg gtggaagaat tgatccctga agcacagcag acattgtcca gcaaacgtca
     6841 ttttgcagtt tttggcgtca tgagtggatt tatcattatg atgattttgg atgtggcatt
     6901 aggataaata aaaaaaatat atatgatata tcaaataggg ccgcgaactt atatcgcgac
     6961 cctatttttt tagtaagtta tatcataatg aagtgaatgg tatcatacat taataatagt
     7021 tttttctatg tgtatttcct ttgatccagc gaacaagcca gatgataagt gcgattggga
     7081 ttagaacttt gaccgcaaac cataaaagcc cgcttaggac actgacaaaa agactgatga
     7141 cgaagctagc tgcaattagc actaaaagaa ccatgaagat atttaagaac atattttttt
     7201 gacgcataca aaaccctcct cttgttcatt ctttactata gaaaaagtat aacagggatt
     7261 gtttaaaaac acctccaact gaagactgat ttttacttca tctacttaaa tgatcaaaca
     7321 attgcttttt ctctggttta tcatctaaaa tagagcggaa aaggaggaga atatcatgcc
     7381 taaaaatcgt cttgaagcat ttacagatgc tgtaatcgcg attatcatga ctattttagt
     7441 tttagaactt catgaaccta acggagacac gtgggaggcg ctcgctgcat taggttaccg
     7501 attttttatc tatattgtca gttttatcat tttagcgatt tattggaaca atcatcatca
     7561 tttatttcaa gttgtacata agataaatgg acgggtctta tgggcgaaca gtttctttgt
     7621 ttttgcgctt tcgctttttc cgtttgcaac ttcatggatc agtgagtaca tcaatagcct
     7681 ggctccagaa atgacttatg gttttgttac cttgaccgct aatattgcgt attatatctt
     7741 ggcaaaagaa cttgtcaaag caaataaagg ccacgagaaa gcattgaagg cgatgttgaa
     7801 taattacaag aaatcgtatc tatcgattgg tttgaatgtg ttaggaattg tcttagggta
     7861 tttagtagcg ccagtagccg tgctgatcgc aaacatcgtg attctagctg cttggatcat
     7921 cccaagtcgt accatcgaat cacaatatcg gtaaaatgat ccgtgtttaa aatttaagag
     7981 aaaaatatag aaaaaataaa taaacgatca aatatccgag tgatactagt attcaaagag
     8041 ggatgcagat ggatcaaaaa agaattcatg taggactgat tactagtttt gtcacgatgc
     8101 ttatttattt ggatcaaggt catttgactt ggacagtagt tggtgtcgta ggatttttga
     8161 cattattcac agcaataaaa aaataaacat ctttatgtaa tctgatgttc tatctattta
     8221 agaaaagcat gtttacagca gtatgattct gttgtaaacg tgtttttatt tgtggaaaat
     8281 ttaattttta gaaaagtgta tcgaaaaata gatagttagt ttttccacag tggataactt
     8341 cgtaagagat aaaaataaaa aattagtcaa agaagggcgt atagatttct tttgatagga
     8401 gatatacaac agaaatgaat aaatattaac gtgttttcaa acagtataaa acggtgtggt
     8461 ttttggaata aaaaaataac atatctcatt ttacactttt gttattttat acttaaaaaa
     8521 taacgacaca gaaaataccg tgtctcagat ttctctacta cttctaaaaa ctactgtttt
     8581 tttcctttga aatgccttca aaaagtagga tcaggcagaa tatactggca gaatcaagga
     8641 gaagggagcc taaataagga gaaaaaccat agaaaatata aagtaattac ttataaaaca
     8701 agctttttat agaaaaataa tatacctgag tagggtataa attaagacgg aattcctttc
     8761 tagattgaga tattgactag gaagaatgtt aagattttaa gcctttaagc taaaaaaaaa
     8821 ccctttttgt taaataaggg gaatatagcc tgtcagtttc aaactgcaag tcgtcctgct
     8881 gcaataatat agtagattag agatgaaaat agattttttt aaaccaatga ctgaataaaa
     8941 agaccgtcat agatacacat tggaatgtgt ctattacggt ctttttttac gaacgatttt
     9001 attgaagacg gacagttcgg gtcaaaaata aaaaagctac gatcagaaat aagaataaaa
     9061 tgtatcccaa aataatggct agacggtcta acttatttgt cgatagatgt ttgtttagaa
     9121 gttgataggc ccataaacct atcagtcctc ctgttgtatt agttatcaaa tcagtgatgt
     9181 ccgctacgcc aataccgaat aggaattgaa tcaattctgc caagaagctt acaaggaaaa
     9241 taatccctaa ttttttagaa aaagaaagtc tcttgaaatt gacattcaaa agcaccccaa
     9301 aaggaaaaaa gacaatcaag ttataagtgg tctcacgtaa taccgtcgta ttttcaaaag
     9361 aaaaagtaga aaaagggacg agattgacat tgctgtaaga ataagtgaga aattgtggaa
     9421 gattaaatga tagcttgaat aaaatcaacc ataatagaaa gaataaataa atccctaata
     9481 aaaattttga tacataccgc atagacatct cctccattta aaaagtatac catcgaaaac
     9541 cgctgaaaaa tcataaaaaa gtatgaagtt taaggaccaa cgtagttatc gtacgaaaat
     9601 tagaagcttt tttttgttgg gaatccggtt tctttaacaa agctaagata gcgagttgct
     9661 tggaaaagtt gctatctttt ttctgtcaaa acgactcgga atgttcgttt tttgttctaa
     9721 tttctccaag ttgtttactt tttgatacta agtttatgaa aaatcaagaa atagttgtta
     9781 tactaggtaa tgctgacaaa cgggattggc atttcatctt ggttcagctt tttttccaat
     9841 tactaggaag gagggataga ttgttcaaga cttattttcg tttcaataaa atacaagata
     9901 atccttttcg tatcatgaat ctgatgctac ttatctttat tgtattatgg ttaggttaca
     9961 taaatcatga ttttttgatt tcaagttttt catcattagg gatctttact tttttctatt
    10021 atcaaaacat accgatgaaa tatttgctga aacgtatgtt tctgatcggt tgcggactgt
    10081 tggttgcttt cgttttgggg atcttatcga cttatgtttt atggcttgaa ccatttgccg
    10141 tagctttggt tgctttttcc agcagattcg tgttgcgatt attccatatt tctaaaccag
    10201 gtggcttgtt ttttgcgatg ctttctgcca tgggaacgag catgcagctg ccaatcgctc
    10261 aattaccgat cgtctcactt tattttttta tgggtgtcgt ttttgccctg attgcggcag
    10321 tgattacaaa attactggat tcacggcctg agcagacaat tgaaaaagca actttaaaag
    10381 aaagatttca cgaagagcct ttagtgatca ttgattctgt tttctacagc gctgccttat
    10441 ttctttccgt ttatgtcagt cacggcttga atctgcataa tccttattgg ttgaccttgt
    10501 catgtgcatc gattttgcta gcagaaaact tggatgcgat gaagcatcga caagtccaat
    10561 atctgattgg atcgatgggt gggttatgtg tgtcggcatt cttgtcattt gtgccgttta
    10621 cgcagttgca gaccattttt ctgatcactt ttctatatgg aatcgcacaa tttctagttg
    10681 ctagaaatta tgcagttgct aatatttttc ttaatccgat ggcgttgatg ctatctacac
    10741 ttattcgtgg agcatacttg atttctttga tcgaatatcg ttttttaggg atcgtcatag
    10801 gaagtttcat cggtttaggt gttgcgtggg taatgacggt tggtttgcaa cattatttaa
    10861 cggttgttcg tcaaaaatta gaatagaagg acaaatatat gtgccaaaag aggttatgac
    10921 aagatttttg tcacaacctc taattcaagg tgttcaaaag ccagattctc agcattaagt
    10981 caaattcaca caaaaatgtg gaaaggaact ttcaaaaaaa ttttatattt attctatcaa
    11041 aaaattttgt ttatgatggt aactgaataa actttcctga gatggtcaat gctttccttg
    11101 aaagtcttac agatattctt tcttcataga aagtttaagt tttatagtta gtgtacaata
    11161 aaagtatgtt tataccatat ggtgtccgga taatcagtca tttaaacaat gaggttcaaa
    11221 gatatgaaaa ggggaaattt gatgaagaaa ataagcaatg aaatccaaat tctggcagaa
    11281 actttaactc ttcccaaagt cgttatagag caaatgattg tacaagcaaa aaacatcctt
    11341 gatgaggtgt ttgagcaata tagtcatgaa ctaatggatc ctaataaagc aaaggcgact
    11401 gcaaagaaaa tagacaaagt ctatgaagag gaaccttttg cagcattagc tatttatcta
    11461 tatgcagctt ctcaaagttg gaaaaaaatt tatcaaccat tgaagattcc aagaactatc
    11521 tatttggcaa caatgaatgc ttttactaga tttattcatg aacatttcca agtaacagga
    11581 agctatcgct ttgaccgtgg tttttggatt tggcattaca ctagtggctt gatctttcgg
    11641 attggtgaat tggagtacga aatgacatat tttccgaaag ggtacacgga tctaagatta
    11701 gaaggaaagc agtgcctttc tcttcatatt ccttccgatg cagatttatc tcagaataag
    11761 ataagagata gttacgaaag tgccattcgt ttttttgaac tttattttcc tgattaccat
    11821 taccaagtga tgtacacaga tacttggtta ctatcaccca atcttactaa atagttgaaa
    11881 aaagagtcaa aaatctgtct ttttgcagcc gattatcgat tattatcagt tgatgaacag
    11941 gacgatagtg gagtcccttg gatctttgga cgagtagatg cccaaataca tgattatcca
    12001 gaaagtacga gtttacagcg acaagcaaaa gagcaattgc ttgcaggcga gcacattggc
    12061 tctggcttag gtattcttac ttcataagtt tagtacttcg aaatagttta aaagagcgtg
    12121 agacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg gcggaagcag
    12181 aagcaactcc ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa
    12241 tttcttctta tttcttggag ttaaacgctt ctgtcccagc ctctttttta gtgttctatc
    12301 gaacacacag tacgttcaat aggtaaacta gctataacaa tagatattaa agttaacgga
    12361 caagagggcc ttttttaggt agctaaaacg taataatgaa aatttatttc cagagatagg
    12421 agtcaatcag ctagtctgat cgactgtttt aacaagttga ctttcatttt aacttttgat
    12481 accatttaaa acacaaatcg taattgtttc gatttgtgtt ttttagcatg actacaatca
    12541 aagaagaaag tgggtcgtcc atatggaaaa aacagatatc tcaagtgcat accgccgctt
    12601 gaaaagccca aatatcaaaa cacgaaaacg tgcgctgaag atcatcaaag atgtgaaacg
    12661 gaatagtggg aaaaggtaag aagaaggaaa cttaattaaa aaaatagccg aaaaacgttt
    12721 ttcggctatt ttcaataatc ggtttttgtg taaggtttcc gaaagaactg ctgttgattt
    12781 tccattttta acatatgata aattggtaga atctttcttt tcaaattgat accattccct
    12841 aattccagtt atatagttaa ctacagaacc acaaagcacg tacgtttata aaagtattat
    12901 ctgtagttat ttttcaattc cctttcaacc tcttattttg accagtagta ttgatcactt
    12961 taccttttaa cacctttgtt cgtttttgaa acatttttag tcaagaatgg aaacgtaact
    13021 acataatcaa aggttttgcg aaatatttca tgaaatattt catgtagaag cgagcaatac
    13081 ggttgcaaat aaccattatc gtttaataag cagttacgaa agctcccaat atctctaacg
    13141 ttcagctcac tccatttctt ttcttcaaaa aaatcagtga ttcgtatctc tttctatttg
    13201 tcgaaaaagt taactttaaa aagtaacgcc gaaaaaatat gcaaaaaaat aaaaaagcag
    13261 ttactattgc tagaataaca tcaaggaata ataagttttg gttttcagat aatagtcgat
    13321 aagttgtgta ttggaatttt agatacagta ataaagtatt tttcatgaaa ttttgtgaga
    13381 atcactatta caagtatctt taataaaatt aatggttaag gaggaactta aagtagaatg
    13441 taaatgaata gttgtagtca gtcgattgtg atactagact caaagttgca ggaataagtt
    13501 tttcaatttg tgattggaag ataaaaaatg aaaaagaagg gctttttttt atactttgtt
    13561 gattgtagtg tttattttcc ctctgtttag ccaagtaaca ttttctatga ctcaaccagt
    13621 ttcaagtgag gaacatgata acctttttcg tgctacagaa gagtttaaga aatatcagga
    13681 aaatattgtt ccggagactc cattagctag tgagccaatt actgatattg atggaaacca
    13741 aataggatgg tatatacagt acgaagttat ggtggtaaat aatgactcaa tagtaaaaaa
    13801 tacagtaggc ttagagctaa caccgattga aaaggtgagt tcattgttaa cgtatattta
    13861 tactgatgaa agtgacgaga ctgagatcat tttaacggat tactcaaaaa ttgctactga
    13921 taaaaagtat atattgtgga tgtagacact agtgaaatac tttatgatat agaaattgat
    13981 gaaaatttac tagatgtagc acaagaaatc gatcatcaaa agcaggaagc tattcgatat
    14041 gctggtagaa aagggaaaag aaaatattta ttacgtgaat gtagcaaaag ttagagaaga
    14101 tgaaaatgag tggaaagaat tcaagagtcg ctactcaatt aactcgactc cgacatttac
    14161 agtttacaga gaaggatcga tagaaaaaac tgttttttgg actaaagaaa gtggaatgtc
    14221 tttagctgaa gtagaagagt ttttggatta tgttagtatg caacagtaag ggagttctta
    14281 ttatgaaaaa ttttgacaca tattatgtta ttgttttttc gatatctttg ctgttaatat
    14341 tactaacttt aatgcagtat aaagagtatc cagagtattt tattttaact tttgtatatt
    14401 tagtaagtag ctacacactt ctatataaat tcagaagttc ttctagagtt tctaaagttt
    14461 gtttcttaat tagcttattg attctataat atttttgatt tatataggaa attaaaaata
    14521 ttatagaatc aacactcaga gaacgggttg gtttttaata gatttaataa gtactgataa
    14581 tgaaatttct cctggttggg aaaacgaagt tggttaccct gtagaagaat tttttcaaga
    14641 ttactttgcg gagtatgccg ataggataat gagagattac gtaacagata aaactcaaga
    14701 agtcatcaat caatcccagt cattaaatga attatctgat aaggcgaaag aagaagcagt
    14761 tgccattatt tcttcaggga ttagtgacta tattgatatg agtggtgaaa tcaattatgg
    14821 cgataaagac aaaattgtac gcatgataaa agcttttatc aaaacttttg atgtgaaggc
    14881 agatcttaag tttaatgata cttacctagt aggaaaatta attaatatcc ttgggacaga
    14941 gaaagatacg gaaacgttta tttcctcttt gtctatgttg tttacagata agcatttttc
    15001 aactcagctt ggtggagagg aactagtgga aatcttttaa ccattacgcc cagcattaat
    15061 aaaagtatat caaaaaagta ctcctgatga tttttaagat tggtttgaga aataatgtga
    15121 aaacactatt tttaatgtta attatagata gagatgaacg cgtcatatgg ttaaaagaat
    15181 gaaaaagtca aagatacact gctatatttc actaaggata tagcagtgta tctttcatgt
    15241 cgtttcactg gcagtttttt ataaaaggag gtgtgcattt cagttgcttt tctttggtag
    15301 ttagttgaat cgtttaagaa ttaacggatg tggcagacag gtgctctgtc caaaaaccag
    15361 taaaatcttt gatggttcca tctttattta gctgtgcctg ttcaaatact gttttgtttt
    15421 taccgaatgg atcaattgca ataattgttc cgatccccgc acgtttggca gcaagtaatc
    15481 ctgagtaagc atcttcgata acaagacaat cagccggagc taagctcagt ttgtctgccg
    15541 ctttaagaaa aatatccggt tgcggtttcc cagggaaaga gccgtcgtca tagacaatct
    15601 tgtcccagtc aaaccagcgg gctaaatcga aaatatcaaa ataaaaagca acattctctt
    15661 tgacagtagc cgtggcaatc gttaatggaa tctgttggtc agtcagctgg tcaagagtcg
    15721 cttcaagccc ttttgtcaac tgaagctcgt tttcattttg caagcaaagc atacgataat
    15781 agctttcttt ttcttctgac aatacggcga tctcagaggc ggtcaaatgc tctgaaatga
    15841 aatgtttcaa aatctccgta ttcgtccgac catgaatatt ttttaaaata tcctcgtctg
    15901 ataaaccatc ttttgtatga tcatgtatca tatgcagcca agcagcttca tgcagatgag
    15961 agtccaaaaa cattgttccg ttgaaatcaa atataatccc tttcatagtt acgctcctat
    16021 agtggtattt tcggttgaat cagcaatttg cagatcgaca tattgtgcat agtgctcttg
    16081 aacctcccgc gagatctggc gatccgtaat gagcagatcg aaatcatgca aattataaaa
    16141 cacataaaaa tcatctcgat tgaatttttt attatcaacg agtagatatt tcttacggga
    16201 attatttaga gcgatctgct gtgcttctcc ttcttcggta ctgtaagtag agatcgcttc
    16261 gttgtgaacg ccatttgaac tgataaatgc ttttgtaaac tttagtcttc ttaaattgtc
    16321 gttggtgatc ggtccgacaa atgcgccggt atttttccgg aaatcaccgc caatcagtag
    16381 aatttctggt ccatctgctt gagcaagaat atcaaaaact ggataattat ttgtaatgat
    16441 ccgaattttc ttgttcagta aatgcgtcgc caatagttcg attgttgtac ctggtcctaa
    16501 gaaaatcgtt tctccctcgt gaatcaagga ggctgctgtg gcagcaattt cttttttttc
    16561 ttgaatctgc agagtggctt tttcagtgtg agaaagttct tgatctaaag tgtaggaaag
    16621 actttgagcc cctccatgga cacgcagcag cttgcccgat ttttctaatt catctaaatc
    16681 tctgcgaacg gtcatatcag aaacatctaa ttgattgatg atttcattaa cggtaaggat
    16741 tcctttttta ttgaccattt ctacgatagt aagcaatctt tcttttttca acattgtctt
    16801 cacctctaat ttaaggataa tctatcatat aaacggacaa cagtcaatct aattcgaaca
    16861 aaagtaaaca tttagggtgg tttttatgtt tgttacaata gattttattg ctgttttctg
    16921 gtttttatga ttatttgtgt ttgtttttgt tgacaaatgt tttggatgcg tttacaatgc
    16981 aatcagttag aaagagcgaa aactgattat agatttgagg agagatgtac atgcttaaat
    17041 taccagaaga ttttattttt ggcggagcca cagcagccta tcaggttgaa ggagccacta
    17101 aggaaggtgg caaaggcgct gttgcttggg atgacttttt agaggaacaa gggcgattca
    17161 gtcccgatcc ggcaagtgat ttttatcatc aatatgcaaa ggacattgaa ttgtgtgaac
    17221 gttttggtgt aaatggtcta agattatcca ttgcttggag ccgcattttt cctgatggag
    17281 ctgggaaacc aaatccagaa ggaatcgctt tttatcatcg tgtgttcgaa gaatgtaaga
    17341 aaagaaacgt aacgccgttt gtgacgttgc atcattttga tacccctaaa cgtttgtttg
    17401 accatggaga ttttttgaat cgagaaacaa tagaagcatt tgtttcctat gccatttttt
    17461 gttttcatga attcaaagaa gtcaaggtgt ggagtacctt taatgaaatc tatccagttg
    17521 caaccaatca atatttgtta ggtgttttcc caccaggaat caaatatgat tttacaaaga
    17581 tcgtcgcctg tcttcataat atgatggttg ctcatgcccg tgtagtgaac tatttcaaag
    17641 aaaacgaact tccgggtgaa attggtgtag ttcactcatt agagacgaaa tatgcggcaa
    17701 cagacgcgcc ggaagataag catgctgcct ttttagacga tgcattgtcg atccgtttct
    17761 tattagatgc tacctattta ggctattatt caactgaaac cttgactgct cttgatgaga
    17821 tatgtgaagc aaatcaggcc agctatcatt ttcctgaaga agatttcgtt gaattgaaaa
    17881 aagcatccac gcgtaatgac tacttaggga tcaatcacta ccaatgccat tttgtcaaag
    17941 cttacgatgg agaaaatgca atccatcata acggtacggg tgaaaaaggg acctctgttt
    18001 ataaagtgaa gggaatcgga gaacgtattt ataaagaagg aattcctcga actgattggg
    18061 actggctgat ttatccagaa ggtctatatg acttgttgct gcgaattaaa agcgattacc
    18121 cacattacaa caaaatctat atcactgaga acgggatggg gtataaagat cagtttgaag
    18181 atggcattat tatggatcag ccgagaatcg actatttgag agtctatttg gaatccttaa
    18241 gcaaggcgat aacagccggg gtaaatgtga aggggtattt tttgtggtcg cttatggatc
    18301 ttttctcatg gacaaatggg tataacaaaa ggtatggctt gttttatgtt gattttgaaa
    18361 cccagaagcg ttacccaaaa gaatcagctt actggtacaa gttggtcagt gaaaccaaaa
    18421 cgattattta ataagtgata atggaggaag aaaagatgaa taaagaggaa ttacaaatgc
    18481 ttggttttga gatcgttgca tattcaggcg acgctcgcag tacattattg aaattattga
    18541 aagaagtaag acaaggaaac tttgaacatg ttgattcggc gttgaaagaa gccgacgaaa
    18601 atttaaccct tgcacataat tcgcaaacaa aaattttagc ggaagaagca tctgggaaag
    18661 agatggagat gggattcatt tttatccatg ggcaggatca tttaatgacc actttattgc
    18721 tgagagatct gattcaagac tttattgtac tgtacagaca gaatcaagga taggagaaga
    18781 acgctatgga tggaatcatt aaacaaatcg aaaaagggaa accatttttt gaaaaaatct
    18841 cacgtaatag ttatttagga gccgttcgtg acggattttt aacagcgatg ccagctattt
    18901 tattttcaag cattttcatt ttgattgctg cggttcctga gatttttggt taccaatggc
    18961 ctgaatctgt ctccacatgg ctatggaaag tctataatta ttcgatgggt gttgtagggt
    19021 tactcgtttc agcaaccact gctcgctgct tggctgattc gatgaatcgt cgcttaccga
    19081 aaaacaaagt aatcaacacc acctctgtga tgctggcctc tgtggtaagt ttcctgttgt
    19141 taagcgtctc tccgattgaa ggtggatttt caacggaata tatggggaca aagggactac
    19201 tggcttcctt tgtcgctgca tttttaacag ttaatttata taagttttgt gtgctgaaag
    19261 atattaccat taaaatgcct aaagaagttc ctggaacgat ttcgcaaacc ttccgagata
    19321 tttttccttt ctctttttca gtatttgcgg ccgttttaat tgatttaggc attcgacaaa
    19381 tcttccaaat ctcatttgca gaagcaatca ttacggtgct gcagccgcta tttacagcag
    19441 ctgacgggta cttaggaatc gctattatct ggggagcgat ggcgctgttt tggtttgtcg
    19501 gtgttcatgg accgtcgatc gtggagccgg caattgcggc aattatctat gccaatgtgg
    19561 aaaccaatct gcaattgttc aattccggtg aacaagcttc taaagtactg acggtgggtt
    19621 tagggaattt tgtaggaact atgggaggaa ctggggcaac attggttgtt ccttttatct
    19681 tcctactctt tgctaagtct aagcagttga aggcagttgg gaaagcttcc tttattccag
    19741 taagtttcgc tgtcaacgaa ccattgctat ttgcggcacc catgattttg aacccttatt
    19801 tttttattcc atttttgtta acacctgttg taaacgtttg cctgttcaag ttttttgtcg
    19861 atgtattggg catgaacagc tttatgtacg ttttgccttg ggcaactcca gcaccgatcg
    19921 gtttggttct aggaacgggc atgggtatct tagcttttgt tctagcagcg gttctaatcg
    19981 tcgttgactt cctgatctat ctaccatttt gcaaagcata cgatgctgtc ttagtagaga
    20041 aagaagcaca aaaagcagag gtgatcgcaa ccgaggaagg tgcttctgcc gatgtacgtg
    20101 aggaagtacc tgtggcagcg agtcaagtta tcagcgatgc aaataaagaa atcaatgtgt
    20161 tggtactttg tgctggcgca gggacaagtg ggatgttagc taatgcttta aaaaaaggtg
    20221 catctgaatc gaaagcaccg atcagtgcaa tggcgggtgc ttatggttct cattacgaaa
    20281 tgctgcagga ttttgatatg gtggtcttag caccgcaagt caattcttac tatgatgata
    20341 tcaaagaaga caccgatcgc ttaggaatca agctggttgc gacaaaagga gcagaatata
    20401 tcggattaac gagagatcct gaaaaagctg ttcagtttat tctgaaccaa tttcaataaa
    20461 ggaggagcca taatgaaaat gggaatttgg ttgcgtaaag ctgttttatt tgcagctgtg
    20521 ttaggggctt tgggggctgt ttcaaaaact gttgaagcat ctgccagcga atttattcgc
    20581 ggagcagata tttcgatcct gtcagatatg gaaaaatctg gtgcaaaata ttatgaagac
    20641 gatatttcaa aagatgcctt agagatttta aaaaataatg gtgtcaacta tgttcgactg
    20701 cgtttgtggc aggatcctta taatagtaat ggagaaagtt atggagcagg gacaaatgat
    20761 ctggaaacga cgattgacct tgcaaaacga gcaaaaaatc tgggacttaa ggtgctgctg
    20821 gattttcact acagtgattt ttgggttgat cctgggaaac agaatcttcc taaagcatgg
    20881 caaggtctga cctttgaaga aatgaatacg gcattgtacg actatacaaa gaacgttctg
    20941 tcggaaatga agcagctgga cgtttatcct gatatggttc agatcggcaa tgaactgaat
    21001 agcggaatgc tctggcctta tggcaagtct tggggagaag gcgggggaga atttgatcga
    21061 ttggctgctt ttttgaagtc tggtatccaa gctgttcgag acactcaacc taaaacaact
    21121 ccagtaatgc tccacttagc tgatggtggc gatacaggag catttacttg gtggtttgat
    21181 gaaatcacga gtcgaggtgt gtcatttgat ttaattggag tgtcgtatta tccgtattgg
    21241 cacggttcaa tgaatgaact tcaaaccaat atgaatgcga tcagccaacg gtatggtaaa
    21301 gaagtgatcg tagttgaaac agcttatggg catacaacag cgaatgccga tactatgcca
    21361 aatgcttttg gtgaagccga agcagctgcg gggggatatg aaccaacacc gacaggacaa
    21421 gcagagtatt tgctggatct tgccgatcgg attcaggcag taccgaataa tcgcggggcg
    21481 ggcttcttct attgggaacc tctttggtac aacggaaatg tgagttgggc aacacaagca
    21541 ggtatgaatt atctcagtgt tcaatccact atgggaaacg aatgggacaa ccaagcgatg
    21601 tttgattttg ctggaaacgc cttgccaagc ttgcgtgcgt ttaaacaagc aggggcgcaa
    21661 ataaatctag ttaaaaatgc aagtttcgaa agcgatggac caacgaccag tccttccaat
    21721 tggcaaacct ggtttcaaca aggaaccgct aagggtacga tcaaaacgga agcgggcact
    21781 ttttttgggg actacaaact aactttttgg caggatcaag catacgaggc atcagcttat
    21841 caaaatgtta gtggcataag caaaggaact tacacattga gtgcctatgt aatgtctggg
    21901 ggagagtttg atactaatca gttatatatc aaaaattatg gtggagcgga actaaatact
    21961 gcggttgtta aaacaagtga atggacaaaa attacaatca aagacattcc aattacaaca
    22021 ggtgtttgtg aaattggggt ttatagcaag gcttcaggaa atgcttggac gaacgttgat
    22081 tttgtttcat tggtgaaaca atagactgcg gatcgtttcg attcaagatc ggaggttccc
    22141 ttcagccaca tcgttagcgt agaaggcagg aatcgaacct gcacctcgcc tttgccaaaa
    22201 ataaccagaa acgatttcca gtcattttta ccaaaacgag taaagcacca gacttcatct
    22261 acactttgta ttatgctaaa gagagttcaa cgatttcgta agaagcaatg aaaaaattgc
    22321 aaaaagttgt gcagataaca taaagtttgt ttaagaaagg gtaaccttag aattccgtcg
    22381 tgaatatgat ttgagcactt agatggatag aaaaaagttc tcggaaaaca gttcgttttt
    22441 cgagaacttc tttttatgat aagaatgaaa caaattaact tatattatag tctcttttgg
    22501 tagggaacca tcttattttt aatgaaaatc aagcatttta gctattgaag aatgttgatt
    22561 tatagggatt ccccctccat attatgttgg ctctttattc ccactccact gttgctggtg
    22621 gtttagaagt gatgtcatac acgatacggt tgacgtgggc tacttcgttt acgatacgta
    22681 cagagatttt ttgcaagact tcccatggga tacgggcaaa gtcagcggtc attccgtcga
    22741 ttgaagttac agcacggata ccgactgtgt agtcgtaagt acgtccgtca cccataacac
    22801 cgacagagcg gatccctgga agaacagtga aatattgcca gatatcacgg tcaagtccag
    22861 cagctgcgat ttcttcacgc aagatagcat cagattcgcg tacgatttct agcttgtctt
    22921 cagtgatttc gccaagaaca cgaataccaa gaccaggacc agggaatggc tgacgccaaa
    22981 cgattgaatc aggcatgcct aactgagtcc ctaattcacg gacttcgtct ttgaataatg
    23041 tgtttaatgg ttcgatcaat tcaaattgca tatcttctgg cagtccacct acattatggt
    23101 gagatttgat cgtctgtgct gtttctgtac cagattcgat aacgtcagta tacaacgttc
    23161 cttgtgctag gaaagagacg ccttcttcac cagcaagttt tgttgcttcg tcatcaaaaa
    23221 catatacgaa ttcattccca atgattttac gtttttgttc tgggtcagat acacctttca
    23281 acttgtctaa gaagcgatct cttgcattga ctttgatgat gttcaagccg aattttccgc
    23341 ctagactttc cattacttgt tccgcttcgc ctttgcgtag cagaccgtgg tcaacaaaga
    23401 tagatgtcaa ttggtcaccg atcgcttttt gtaaaagaac cccaacgaca ctagaatcaa
    23461 cgccacctga aagaccaagc aagacttttt tgtcaccaac ttgttcacgg attttttcta
    23521 cttgcatatc gataaagtta tccattgtcc agttaccttc acagtgacaa acatcaaagg
    23581 caaagtggcg cagcaattcg attccgtatt cggaatgacg tacttctgca tggaattgga
    23641 taccataaaa attacgtgtt gtgtcttgga tagaagcaat cggacaatct ttgcttgttc
    23701 cgactgtttc aaagccttca ggaactttag ttaccaagtc accatggctc atccatactg
    23761 tttgttgttt tggtgtttct ttaaacaaga cagcttgatc gcttgttact tctaacaagg
    23821 ctttcccata ttcgcggttt ttagcaggct ctacggaacc gccaagtttg tacgtgatca
    23881 attgcatacc atagcaaatc cctaatacag gaatacctaa attgaagatc tcaggatcga
    23941 tcccaaatgc attctcatca tatacactgt ttggtccgcc agaaagaatg attccctttg
    24001 gattcatcgc ttttacttct tctgcagtaa tgcggtggct caataattcg gaaaagactc
    24061 caagatcacg aatacgtctt gtgatcaatt ggttatactg actaccgtag tctaatacga
    24121 taatcttttc aacgttcgtc atatcggcaa cgtttgtcaa atttattacc cctatccctt
    24181 taaattttat ctcaaaatag tcaaactatt ttaaaatgct aatattttcg catgaaaatc
    24241 gaatcgatga tatgattttc agttttatgg agaattatat cggctcttcc tcgtgtaggc
    24301 aagatatatt cctgtaaatt tttcaaattc acatttttcc atacatcttt tgccatttta
    24361 aaggcttctt cacgatctcc gatagcaaaa cgataataat aattctctgg attttggaaa
    24421 gccgtatcca gtaacgcacc aaaacgatcc aaataccatt tttcgatcag cacgggatcg
    24481 gcatcgacat agacagaaaa atcaaaaaag tcgctgacgt agatttgctg attcgcaggt
    24541 aattgaagcg tattgatccc ctcaacgatc aagatatcag gctgctggat caattcgtac
    24601 ttgtctggaa tgatatcata cacatcatgt gagtaaacag gtacttttat ctcttcctga
    24661 ccagatttta cacggttcaa gaaatcgatc agcatctcca tatcataact ttcaggaaat
    24721 cctttacggt ccatgattcc acgctctttt aatatacgat ttggataaag aaatccgtca
    24781 gtcgtgatca gctgaacgtt tcttcgtttg aaggtacgag acaaaatcgt ctgcagcagt
    24841 ctggctgttg tactttttcc aacggctaca cttccggcga tcccgataat aaaaggcggt
    24901 acttctacgt atttatgaag aaataaacct ttactcaaga acaaagattc aaattctttc
    24961 atataaagat aaatcagatg agcaagaggt acatagatat ctcgaacatc ctgcatggaa
    25021 atgcggtcat tcaaactttt gatattatct agttcatctt gtgtcaaagg tgcttgccca
    25081 ttacgataaa attctcgcca ttcttctctt gccacacggt agtagttcat tggatcattc
    25141 atattagcct cctgcgttct cccatcaatg gaactatttt aacacaaaat aaacgcaaac
    25201 cccaccactt aagagagatt gccgatcttt ttgtataatc tttataatag aaagaaacgg
    25261 agtcttattc ttgacttgcg cacggataca taaaaggata gactgttgtc atgaaagaaa
    25321 tgagaaggat ggaagacatg acaaatcatt actattctga acgcccagat acggcgcacg
    25381 attttgaaca atgggctttt gagttaaaag gcagaacctt tcaatttgta acagattctg
    25441 gtgtattttc acgtgaaaca gtggattatg gttcacgtgt cctgattgat gctttcgatt
    25501 gggaagagtt acctgatgaa ggaaagatct tagatgtggg atgcgggtat ggaccgattg
    25561 ggttagcttt ggcatttgcg acacagcggt ttgttgaaat ggtcgacatt aatgaacgag
    25621 cggtaagtct agcgcaagga aacgcaaagc gtaaccaaat cgaacatgtt gatatccatc
    25681 aatcgaatat ctatgaagca gttcatgaag aaacctatgc agctatcgta agcaatccgc
    25741 cgatccgtgc tggaaaaaaa gttgtacatg aaattttgac aggcgcatat cctcggttga
    25801 agaagggcgg tacactgaca attgtgatcc agaagaaaca aggagcgccg agtgcgcaaa
    25861 agaaaatgga agaaacattt ggtaatgcag aaatcgtcac aaaagataaa ggatattata
    25921 tcatcaaaag tgtgaaagaa taactagctg aaaaatagtg gagcgacgaa agagtggaac
    25981 tgggcgtgca tgtacgggta cgtatcagtt ctttttttgt tgagaagttt ttaaaaacaa
    26041 agaactattt tttcttcaaa aagaaatagt aggaacagag tcgttgtttt ttacgataaa
    26101 ataaataaga tacagaactt gtatagaagg ctgccaagtc ggtagataaa ggaggttttt
    26161 atcaaaaaac atactttcct tttttctcta ttcttttctt gggggttttg gtacactgat
    26221 agggaattta gatagaaggg agccaatagg atgaagctga ccatcagaga tatcgcagaa
    26281 atggcaggcg tatcagtaac aactgtctca cagattttga ataacaaagg aagcagattt
    26341 agtgataaga caagaaaaaa agtgttgtcg atcgtgaatg aacatcatta taagcccgat
    26401 tatttcgctt caaatgtgat caatcgacat tcaaagacca tcggaatgat cgtccctgat
    26461 gtcactgatt tcttcttctc caaactgatc gaaggtgtag agagctattt gaatcctttg
    26521 ggctatgtga tcatgttatg caattctcgt catagccaag aaaatgagat caagtattta
    26581 caagagcttt cccaccgctc agtggacgga atcttgctgg ccacaccaaa tattttacct
    26641 gagaaatatg cattagacag cggtttttat caaaaaatgc caatcatttt aatcgatcgc
    26701 ggattgaatc gtcgggataa cggacggctt atcgtaaagg aatatgaggg cgcctatcaa
    26761 gctgtttcgc tgtttattga aaatggccat acgaagatcg gcatgctgaa ggaaaccact
    26821 ggatactatc aattagaaga acgtttcaac ggatatcgtc atgcgctgaa agatcatggc
    26881 ctgcctttta atgggaaatt tgttgaacat ggagagttga ctgtccaagg tggatatact
    26941 gcatcaaaaa aattactcaa acataaagat atcacggcta ttttttgcgg gaatgatgcc
    27001 atggcgatcg gatgctatca ggcgatcgat gaattgggca aaaaaatccc agaagatatc
    27061 tcagtcatag gattcgatgg attaaagctg tcagaataca tgattccgca attgacgaca
    27121 gtgcagcagc caagttttga tattggattt tatgctgcac gtttcctgat cgatacgatc
    27181 gaatttcctc aaagaaaagt tccgaacaaa gtgttcgaaa caaaattaat tattcgtgag
    27241 agcgtaaaag tattgacaaa gggctaactc ggcggtatat tgcttatgta agcatttaca
    27301 aaggctagtt agtgcttttt ttatttttag taaaccagtt tactaaagtg gtttaccttt
    27361 cctaaaaata atgtataata ccgaatgaag acaaggagga tcaggcaatg cgaatggtgg
    27421 atctgattga aaaaaagagg gatggaaacg agctgtcgaa agaggaaatc gagtacatcg
    27481 tcacgaacta tacgaatgga aagattccag attatcaagt cagtgcatta ttgatggcaa
    27541 ttttttatca agacatgaca aatgaagaaa tcacgaactt aacattggcg attgctaatt
    27601 ctggtgatgt catcgatttg agttctcttg aaggaataaa ggtagataag cattcgacag
    27661 gtggcgttgg tgacacgacc acattgattt tagcccccct agtagctagt gtcggagtga
    27721 cagtggctaa aatgtctgga cgcggtctag gttacacagg tggtacacta gacaagctag
    27781 aagcaattcc cggatttcag atcgagcttt cggatgaaga atttgtacgc attgttaatg
    27841 aaagtaaagt agcggtaatt ggtcaatcag gaaacttggc accggcggac aaaaaattgt
    27901 atgccttgcg tgacgtcaca gcgaccgttg attctcttcc gctgattgcc agttcgatta
    27961 tgagtaaaaa aatcgcggca ggagcagatg caatcgtttt ggatgtgacc actggagacg
    28021 gtgctttcat gaagaacatc gaagatgcaa gacgtctggc aaagaccatg acgagcattg
    28081 ggaaactggc taaccgagaa acagtggcgg ttatctcaga tatgtcagaa cctcttggcg
    28141 aagctatcgg aaatagtcta gaagttgtcg aggcaatcga aacattacaa ggaaatggtc
    28201 cagaagatct agtagaaatg tgctacgcct taggaagcca aatggttgtt cttgctggca
    28261 aagctaagac aaccgatgaa gcaagaacgt tgcttcaaga agctctggaa tcaggtaaag
    28321 cgttagcaaa attcaaagaa atgatccaaa atcaaggggg agacccagcg atcgtggaac
    28381 atccagaacg aattttgact gcacgctaca caatggagct tcctgctaaa caaagcggag
    28441 ttgtgtcaaa aatcgtggca aatgaattag gaattgctgc aatgatgcta ggtgctgggc
    28501 gtaaaaccaa agaagaagat attgatcacg ctgttggatt gaaactgcat aaaaaaattg
    28561 gtgacactgt gacaaaaggt gagtcactat tgacgatcta tagtaacgac gaagaaatca
    28621 cttctgtcat tgaattatta tataaaaata ttgaaattgg tgaatcggca atgaagccaa
    28681 cgttgattca tgacatcatc acagaataaa ggaaaaaagc ggactttacc gttttaaaga
    28741 aaaggggaaa agaaatggaa ctaaaccgaa tgatcgacca tactattttg aaagcagacg
    28801 catctaaaga agctgtgatg caaattatcg aagaagcaaa aaaataccat ttttattctg
    28861 tctgcatcaa tccagcatgg gttgcgctag cgaaagaaca attacaaggt gagccagtag
    28921 ccgtatgtac agtaatcggg tttcctctag gagcaaacac acccgagaca aaagcatttg
    28981 aaacaacaga tgccatcaat aacggtgcag atgaagtcga tatggtcatc aatatcggtg
    29041 ctttgaaatc aaaggatgat caacaagtcc aaaaagatat cgaagcagtt gttgaagcag
    29101 caaaagatcg tgcgcttgtc aaagttatta ttgaaacatc gttattagat agagaagaaa
    29161 tcatccgagc ttgcgaaata gcaaaagctg caggcgctga ttttgtcaaa acatcaactg
    29221 gtttctcaac aggcggagca aaggtagagg atgtccgttt gatgcgtgaa actgtaggac
    29281 cagaaatggg tgtcaaagct tccggcggta tccataacga agaagaagca atggcaatga
    29341 tcgaagctgg tgctacaaga atcggaacaa gcgcaggagt agcaatcgta tctggttcaa
    29401 ctggtgaagg ctattaattt tttcaaagga gaagctcatg gcagaactca aacaagaatg
    29461 gatcaacaca gctattgaag cgttagataa agcctatgtt ccttattccc attttccggt
    29521 aggcgcttgt ctcgtaacgg aatcaggcaa gatttatcaa ggcatcaaca ttgagaatgc
    29581 atcatttgga ttaacgaatt gtgccgagcg cacagctttt ttcaaagcag tatctgaagg
    29641 agaaaaaaca tttacgcatc tagtcgtagc ggggcacacc cccgatccta tctctccttg
    29701 tggtgcatgt cgacaagtaa tggcagaatt ctgttcccct gatatgccgg ttacgctagt
    29761 aggagacaat ggtgtgacga aggcaacgac cgttcgtgca ctgctgcctt atgcatttac
    29821 tgaaaaagat ctataactca tgttcattca gtcagctgac tgttgaaaaa taaaacaatt
    29881 attttaggag gctttacaga aatgaaaaaa gcaaaattat ttggtttagg tgctgtcgca
    29941 ctagcagcag ggttattttt aggtgcatgc ggcaacaacg gctctactga ttcatccggt
    30001 ggcaaaagct catcagacac aacaactgca gcattgatca cagacacagg tggtgtagac
    30061 gaccgttcat tcaaccagtc agcttgggaa ggactagaaa aatgggggaa agatcaagga
    30121 ctttcacgag gaaatgatgg cttccaatat ttccaatctt caaatgaatc agattacatc
    30181 ccaaatattg accaagcatt gaatgctgga ttcaaaacga tttttggtat tggttataaa
    30241 ctaaaaccag cgatccaaga acaagcaaca aacaatacag gtacgaactt tgttattatc
    30301 gatgatgtca tcgatggatt agataacgtt gtatctgcaa ctttcaaaga caacgaagct
    30361 tcttatcttg caggtgttgc agcagcttat acgactgaaa caaacgtagt cggctttatc
    30421 ggcggtgtaa aaggtgaagt aatcgatcgt tttgatgctg gattcaaagc cggtgttgat
    30481 gcaggtgcga aagaattagg aaaagaaatc aaagtattga atcaatatgc tggtgacttc
    30541 tctgctccag ataaaggacg ttcaattgct caaggtatgt atgcacaaaa cgcagatatc
    30601 atcttccatg cttcaggcgg tacaggtaac ggtgtattcc aagaagcaaa atcattgaac
    30661 gaatcaggcg acaaaaaagt ttgggttatc ggtgttgacc gcgaccaatc agacgaaggc
    30721 gaatacacat taaacggcga aaagaaaaac ttcacattga catctacact gaaagcagtt
    30781 ggaacagtag tagaagattt ggcacaaaaa tcagcggacg gtaaattccc tggcggcgaa
    30841 cacactgttt atggattgaa agaagacggt gttggtctaa cagaaggaca actttctgac
    30901 gaagcgaaaa aagcagttga tgaagcaaaa gaaaaaatca tttcaggtga cgtaaaagtt
    30961 ccagaaactc ctgaagaaaa ctaaaaccat ttctatagcc ttgttagaca catgtttgac
    31021 aaggctatgg tttgattaga acaagaattg ttcatttatc caagctgcct tgtgacagtt
    31081 tggataaggg aacaactccc tgaagtaatg tttgtttcaa aagaagaacg ctcactaagc
    31141 gtgaatctta gtcaatcgtt gccctagata gaaatcgtta ggatgtgaaa aaaaggtggt
    31201 tcaagaaaat attgtcatag aaatgcagaa tattaccaaa gaatttggaa cattcaaggc
    31261 aaatgacaac atcaatttac aagtaaaggc aggagaaatt catgcgttgc ttggagaaaa
    31321 cggtgctggc aaatctacat tgatgaacgt gctttccgga ttattagagc cgacatcagg
    31381 gaaaattttg atgcgtggga aagaagtaaa gatcacaagt ccgaccaaag ccaaccaatt
    31441 agggattggg atggtccatc agcactttat gcttgttgat gcctttactg taacagaaaa
    31501 catcgtgttg ggaagcgagc ctagtcgcgc agggatgctt gaccataaaa aagcacgaaa
    31561 agagatccaa aaagtttctg aacagtatgg attgtcagtc aacccagatg cttacgttcg
    31621 tgatatttca gttggaatgg aacaacgggt agaaatttta aaaacacttt accgaggagc
    31681 agatgtactg atttttgatg agccgacagc tgtattgacc cctcaagaaa ttgatgaatt
    31741 aatcgtgatc atgaaggaat tagtcaaaga aggcaagtcg atcattttga ttacgcataa
    31801 gttagatgaa atcaaagcag tagctgaccg ctgtacagtt atccgccgtg gaaaaggaat
    31861 cggtacagtc aacgttaaag acgtaacctc acaacaatta gccgatatga tggttggaag
    31921 aacggtttca ttcaaaacga tgaaaaaaga agcgaagcct caagaagtcg ttttgtctat
    31981 tgaaaatcta gtggtgaaag aaaatcgtgg attagaagcc gtgaaaaact tgaacttaga
    32041 ggttcgtgct ggcgaagtac ttggtatcgc tggaatcgat ggaaacgggc agtcggagtt
    32101 gatccaagct ttgactggtt tgcgaaaggc agaaagcgga catatcaagc taaaagggga
    32161 agacatcact aataaaaaac cccgaaaaat cactgaacat ggtgtaggac acgtgccaga
    32221 agaccgtcat aaatacgggt tagtcctaga tatgacatta tctgaaaata tcgcactaca
    32281 aacctatcat caaaaacctt acagtaaaaa cggcatgctg aattattcag taatgaatga
    32341 acatgccaga gaattgatcg aagaatatga tgttcgaaca acgaatgaac ttgttcccgc
    32401 aaaagcttta tcaggcggaa atcagcaaaa agcaattatc gctcgtgaag tcgaccgaga
    32461 tcctgatctg ttgattgttg caaatccaac tcgtggactg gatgtaggtg cgatcgaatt
    32521 tattcataaa cgtctgatcg aacaaaggga caaatacaaa gcagtgttac tgatcagttt
    32581 cgaattagaa gaaattttaa atgtttcgga tcgtattgct gttatccatg aaggagaaat
    32641 tgtcggtatc gttgatccga aagaaacatc tgaaaatgaa ttaggtctat tgatggcggg
    32701 ctatacctta gaagaagcac gaaaagagtt agaaaagggg gctggcgcac atgaatgatc
    32761 gcagcgaaaa attccgtaat ttattagtgc ctgtactttc agttgtttta ggattgatct
    32821 taggcgcgat attgatggct gcttttggtt ttcagcccat ccaaggttat tcttctatgc
    32881 tagatgcagc ccttggttcg caaagaggga taggtgaaac gttaaggcaa gcaacgccgt
    32941 tgatttttac agcattaggt ttttctgtag ctaactcagc tgggtttttc aatattgggc
    33001 tttctggaca agcactttgc gggtggatcg tcagtgtctg ggtggcactt gcgtttccag
    33061 atctgccaaa agttgttttg cttccgttat gcgtgatctt aggtgcctta gctggtgcga
    33121 tggcagcggc tgttccaggt ttacttcgag cattttttgg aacaagtgaa gtgatcgtaa
    33181 caatcatgat gaactatatc ttattatacg taagcacatt tgctttacat gaagtgatgc
    33241 ctgaaagcta tcgctcaagt ttggactctt ccaatatgat cagtcaaaat gcctcactgc
    33301 ggttaccatg gttaacacaa gcttttggcg gctctcgcgt aaactcagga ttatttttag
    33361 caatcatcgc gcttattttc gtttggctgc tgatgaagaa aacgacttta ggatttgaaa
    33421 tccgttctgt tggattgaat ccttttgctt ctgagtatgc aggaatgagt agtaaacgta
    33481 cgatcgtttt gtcgatggtc atttctggcg ctttggcagg gcttggcggt gtggttgaag
    33541 gattaggaac gtatcaaaac ttctttgttc aaacgacttc gctatctatt ggatttgacg
    33601 gaatggctgt ttccttgctt ggttctggat cagcaatcgg tattctttta tcagcgatct
    33661 tgttcagtat tttgaagatc ggtggacttg gcatgcagac aggagcaggt gttccgtttg
    33721 agatcgtcaa tgtatcgatt gctttgatta tcttctttgt cgggatcaac ttcctgattc
    33781 gtttcgtcat ggctaaagtc ttccaaggga aaaaacaaga agagatcgtt gcgacaatag
    33841 aatcgaaacc aacgaatcaa aaccatgaag ggggcgaact ataatgtcga ctatcgaatt
    33901 aattgccttg atccttactt ctacactggt ttattcaaca ccgctaattc taacgtcact
    33961 aggcggaact ttttctgaac gaagcgggat cgtaaacgta gggctagagg gaatcatggt
    34021 catgggtgct tttagttcca tcgttttcaa cttgacgatg agtccatcac ttggttctat
    34081 gacaccatgg atcgctattt tagtgggtgg cgtcgtaggt atcctatttt ctctgattca
    34141 cgcagtagca acagtcagtt tgcgtgctga ccatattatc agcgggacag taattaatct
    34201 gatggcacct gctttaggtg ttttcttgat caaagcatgg tatggtaaag gtcaaactga
    34261 taacatcacg cagaatctgg ggtatttttc tttcccagga ttagcagata ttccggtcat
    34321 tggaccaatt ttctttaaaa atacattctt acctgcttat tgtgctattt tgattgctgt
    34381 cctagcttgg gtgatcatct tcaagacgaa attcggcttg cgcctccgtt cagttggtga
    34441 aaatcctcaa gctgccgaca cattaggtat caatgtatat gctatgcgtt attcaggcgt
    34501 actgatttct ggcttgttag gcggtatggg cggtgctgtt tttgctcagt cgatttccgg
    34561 taacttttca gctggtacga tcgttggaca agggttcatc tccatggctg ctatgatctt
    34621 tggtaagtgg aatcctttag gggcaatggg tgcctcattg ttctttggtt ttgctcaaag
    34681 cttgagtatc attggcgcac aattaccagt tatttcttcg attccgccag tattgctgca
    34741 aattgctcca tatttgctga caatcattgt gctggttgtt ttcttaggga aagcttctgg
    34801 tccaaaagca aacgggaaga actatatcaa atcaaaataa tcttttagcg aataaaaagg
    34861 gtgggttacg ggaagtgttt ttcgtctcgc cctttttgtt ttaaaggcaa taaaaataaa
    34921 aagaaagaag gaataatcat gtttaaacgc gtacatctga tcgtcatgga ttcagtagga
    34981 attggagaag caccagatgc cgataagttc ggtgatgttg gttctgatac attaggacac
    35041 attgcgaaag aagcaggatt gaacgttcca catttagagc agcttggttt aggaacgatc
    35101 cgtcctcttg aaggtgtgga atcagtgaaa gatcatcaag gctatgcgac gaaattagaa
    35161 gaagtttctg tagggaaaga tacgatgaca ggccactggg aaatcatggg attgaatatc
    35221 aaaactccat ttcgtgtgtt tcctaatgga ttccctgatg atctgatcaa acaaatcgaa
    35281 gaattttctg gccgtaaagt cgtatgtaac aaaccttata gcggaacagc tgttattgac
    35341 gattacggtg aacaccaaat gaaaacaggc gatttaattg tatatacttc tgccgatcct
    35401 gtccttcaga tcgcggcaca tgaagaaatc attcctttag aagaattata tcggatttgc
    35461 caattcgttc gtgatataac aaaagatgat ccgtatatga ttggccgaat cattgcacgt
    35521 ccgtacgtgg gtgaaccagg agacttcaca cgaacaagca atcgccatga ttatgcgttg
    35581 aatccattcg gacacacagt ccttgattct ttgaaagaag caggaaagga cgtgattgct
    35641 gttggaaaga tcaacgatat tttcaacgga caaggaatca ctgaatctgt ccgcacgaaa
    35701 agcaatatgg atggcgttga ccagctgctg aaagttatga aaaacgattt ttcaggaatc
    35761 agctttacca atttagtaga ttttgatgca ttatacggtc atcgtcggga tgtagtcggc
    35821 tatgcgcatg cgattgaaga atttgactta cgaattccag aattgcttga agcgatggaa
    35881 gaagacgact tgctaatgat tacagccgat cacggaaacg atccgacgtt tactggtacc
    35941 gatcatacgc gagaatatgt accgttattg gtttacagta aaaaaatgaa agaacaaggg
    36001 gcattgcctc aaggatatta ttcagatatt tcagcaacga ttgctgagaa ctttagtgtt
    36061 tcttcgactg aaaatggcga aagcttcttg aaattattaa aataatcttt ttaaatagga
    36121 gaaagcaaat gaccaagtta agcgagatgt tacaagaaac gacccagttt atccttgata
    36181 aaggagtaaa agaaattgat tttggtttga ttctaggttc aggattaggt gaattagcag
    36241 atgaaatcga agatgctgta atgatttctt atgatgagat cccgttcttt cctgtttcga
    36301 cagtagttgg gcatgcagga aagctagttt atgggtctct ggcaggcaag aaagtgttag
    36361 ccatgcaagg gagattccac tactatgaag gtcattccat gcaaacagtg acctatccag
    36421 tacgtgtgat ggccggacta ggggcccatt cagtcattgt gactaatgca tgtggtggtg
    36481 tcaacgaatc atttgtacca ggagatttga tgctgattac cgatcatatc aattttactg
    36541 gtgaaaatcc tttgatcggt ccgaatgaag atgcaatggg ccctagattt ccagatatga
    36601 gtcaagctta tacgcctgac tatcaagcaa cagcaaaagc agcagcagaa aaactaggtt
    36661 tggatcttaa agaaggcgta tatatgggct attctggacc aacttatgaa acgcctgcgg
    36721 agatccgaat gtctcggaca ataggagccg atgcagtagg gatgtcgact gttccagaag
    36781 tcatcgtagc agctcacagc ggattaaaag tgttaggaat ctcttgtatt accaacttag
    36841 cagcaggtat gcaagcgaat ctgagccatg aagaagttgt agaaacaact cagagagtca
    36901 aacaatcttt taaagcattg gttaaagaaa cattgacatt attgtaatag gaggaacaat
    36961 aaaatgagtg tgcatattga agcgaaagaa ggcgagatcg ccgataagat tttacttccg
    37021 ggagatcctt tgcgagctaa atatattgcg gagacttttt tggaagatcc agtttgttac
    37081 aatcaggtac gcggtatgtt aggctttacc ggcaaatata aaggagaacg tatttctgtt
    37141 caaggaacag gaatgggaat gccatcagcc accatctatg cccatgaatt gatccagtcg
    37201 tatggtgtaa agaaattgat tcgagtaggt acatgcggtg cattgtcaaa agacgtccat
    37261 gttcgtgatt tagtactagc acaaggtgca gcgaccagtt cttcgatgat cgaaaaaaat
    37321 ttccaagcct ttcattttcc tccaatcagt gatttcaatc tattactgaa agcatatgag
    37381 atagctaaag aaaagggcta tacggttcat gtaggaaatg ttctttcgga agattctttc
    37441 tacaaagatg atttgacaga gacgttccaa ttagctgaac ttggcgtttt aggagtggaa
    37501 atggaagcag cggctcttta ttacttaggc gcgaaatacc atgtgcagac attaagtttg
    37561 atgaccgtta gcgaccattt gatcaccggt gaagaaacaa cagctgcaga acgtcaatct
    37621 acgttcaatg aaatgatcga agtaggattg gaaacagcga tagcagaata agaaggaaat
    37681 gaaaaagctg ccctgcatta agtaataaga acatccaagc aaaaatagct cctcatgttt
    37741 ttgacttggc tggacttatt attgcaaatg taagcttttg aacaccgttt accaagaggt
    37801 tgtgaatctg ccgtttaact ctgagtcaca aggaacaatt tcaaaaaaca gcttttcatg
    37861 tttgatgaat attgtgactt gtgaccgaga agttggcagg agaaaaccgt ttatacgaaa
    37921 aaagggactg tgacaaatcg tgtcacagtc ccttttttat agtgtttttt ataggtagta
    37981 ttttttagca actgttaagt catcatttaa ttcataaaca agtggttgac ctgttggaat
    38041 ttcaagatcc atgatgtctt cgtcagagat accttcgata tgtttagcta gggcgcgtaa
    38101 tgagttacca tgtgcagcta ctaaaacagt tttgttgtct agtaatgctg gagcaatttc
    38161 atcttgccag aatggcaaag cgcgttctaa tgtcactttc aagttttctc caccaggaat
    38221 atcgcgtttg tctaacattg cgtaacgacg gtcatttgct gcagaacctt cgtcagtttc
    38281 ttccattaat ggaggaagag tatcgtaaga acgacgccag atgtgcactt gttcgtcgcc
    38341 gtatttttca gctgtttctt ttttgttcaa accttgtaat ttaccatagt gacgttcgtt
    38401 taagcgccat gatttgattt gtggtaccca aagttgatct gaatattcta agatcaagtt
    38461 acatgttttg attgcacgag ttaaaacaga agtgtaagca acatcaaatt cgattccagc
    38521 ttctttgatt ttgcgtccgc cttcttttgc ttcttcgatt ccttctggtg ctagatttac
    38581 atcagcccaa ccagtaaatt ggttcaatgc attccattca ctaagaccgt gacgagaaaa
    38641 aactaatttt ggcatgttta gctctccttt taagataggt ttatatttct gaaattttca
    38701 gttctgtttt attgtacaat tttttggaag agatttccat cgaaaaatta gataagattc
    38761 tatgagaaaa caagtagaga atataaaaaa acgaacagtg cccacgcaac tgttcgtttt
    38821 taggtattag atgcataatt gctcgatttc tttttcaggt aaattgatat atttataaca
    38881 tgttccaact gaaacaccgc attttgaaga aataaattgg atcgtttctt ttttttcata
    38941 atagagacgt cggatttttt tgatcgtttt tgcatcgatt tttggtcttc cgccgatttt
    39001 tcctttttta cgagcttcat ctaatccaac taatgtacgt tctttgatca atgcgcactc
    39061 catgtttgct aggttttcca ttaattggaa atagattttt cccatttcgc gcgtatcgat
    39121 tccttcatca agactaatca agtgcagccc gcgatctttg aataattgcg tgagttctgt
    39181 taattgccgc gtagatttgc ctaatcgatg aagttggcaa atgactagtg catcgccttg
    39241 ttgcatgtca gcgatcactg actccaaatg aacagcagta gaatcatttt gttccacttc
    39301 atatgtctca tggaaaattt cttgacaacc aaagtcagat aattgatcaa cctgagttgt
    39361 taaatcaacg tcatttatgg ttgtgcgtgc ataaccaata attttcatgg tgattccttt
    39421 ctagcagtga ttttctaact ggatataaca gtaaaaatgt agcataaagt acgtcaaaaa
    39481 gatagttttt tcgcaaattt tccgctgtct ttttttattg gatgatcaag cagtttttct
    39541 ttatttacga cggattgcgt caagaatctc aacgcctttt tctgagccga caactacttt
    39601 agaaaccatg tttaggaaaa gcccatgttc tactacccca acaagttcat ctaaataggt
    39661 agctaagctt tctggctgtt cgatgacttc tagatgcaga tcaatgatgt aatgaccacc
    39721 atctgtggtt aagacttctc cgttttcgtc tgtacgcaag acgggttgga atcttttttc
    39781 ttcaaaaata tgaagtagct gttgggagcc ataagggatc acttcaactg ggagtgggaa
    39841 cttcccaagt ttatcgacta atttagaaga atcaacgatc caaatcgttt cttttgagta
    39901 cgtagcgact attttctcga aaagcaaagc agcgccgccg cctttgatcc cttgaaaatc
    39961 agcactgatt tcatcagcac cgtctattgt caaatcgact acggggactt catcaatact
    40021 ctttaacggt attcctaatt tttccgcttg ttcttttgtc gcatttgaag tagtaactcc
    40081 tgtgatacgt aaaccttctt cacgcatacg gcgaccaatc tcttctacca tataataggc
    40141 ggttgaaccg gtcccgagac ctacgatcat cccgtccttt acgaatttcg cggcttcaat
    40201 gccagctaat tgttttaaat tcatctttgc gtccaccttt ttttagtttg agcatcgtgc
    40261 tttcctcact attttagcgt gatttacggt aaaaagatac cattcttttt tattccaata
    40321 aagctttttc aaaaaaaatt gcatctcttg ttgacatgtt ttttcattcg tgatattcta
    40381 acaaaggtgc tttatctaca ggtttctgtg aagaaacgtg ctgactgttt atcaaagcat
    40441 atttgataag gatgcagaca ttgcatttat tttttatcac aaaaaaagaa ccacctggat
    40501 gtgtggaact agacgcaaat cgtgggaagg aggaaacaag gaatgcctac aattaaccaa
    40561 ttagtacgta aacctcgtaa atcaaaggtg gaaaaatcta attcacctgc tttgaacaaa
    40621 ggatataaca gctttaagaa aactcaaact aacgtaaact cacctcaaaa acgtggggta
    40681 gcaactcgtg tgggtacaat gacacctaaa aaaccgaact cagctttacg taaatatgca
    40741 cgtgttcgtt tgtcaaactt aattgaagtt actgcttata tcccagggat tggtcataac
    40801 ttacaagaac acagtgttgt attattacgc ggtggtcgtg taaaagactt accaggggta
    40861 cgttaccata tcgttcgcgg tgctttagat actgctggtg ttaacgaccg taaacaaagc
    40921 cgttctaaat acggaactaa aaaacctaaa gcttaatttt aattattaaa caaatgaaac
    40981 ttgcttctac tatatagaag taattactat gaacaatatt tcggaaggag gagttacgga
    41041 tgccacgtaa aggtcctgtt gcaaaacgtg atgttttacc agatcctatt tataactcaa
    41101 aattagtaac tcgcttgatc aaccgtgtaa tggttgacgg aaaacgcggt attgcagcta
    41161 acattatcta taatgccttt gacttgatca aagaatctac aggtaacgat ccattagaag
    41221 tgtttgaaca agcaatgaaa aacgttcaac ctgttcttga agttaaagca cgtcgtgttg
    41281 ggggttcaaa ctaccaagta ccagttgaag ttcgccctga acgccgcgtt actctaggtc
    41341 ttcgttgggt agtaaactac gctcgcttgc gcggtgaaca cacaatggaa gaacgcttag
    41401 caaaagaaat catggatgct gcaaacaaca caggtgcttc tgttaaaaaa cgtgaagaca
    41461 ctcataaaat ggctgatgcc aaccgtgcgt ttgctcacta tcgctggtaa gattctctct
    41521 gtttggtcgg cttttataag tcgacctcag atagtgtcct tatttttagc aactagatag
    41581 taaagagagg agaaacaagg agaatgacca gagaattttc tttacaaaac actcgtaata
    41641 tcggtatcat ggcccacatc gatgctggta aaacgactgc tactgaacgt atcttgtact
    41701 atactggtaa aatccataaa attggtgaaa ctcatgaagg agcttcacaa atggactgga
    41761 tggaacaaga acaagaacgt ggtattacga tcacttctgc tgctactaca gcacaatgga
    41821 agggtcaccg aatcaacatc atcgacacac caggacacgt agacttcaca gttgaagttg
    41881 aacgttcatt gcgtgtactt gatggtgctg taactgttct tgatgcacaa tcaggtgttg
    41941 aacctcaaac tgaaacagtt tggcgtcaag caacaactta tggtgtacct cgtatcgtat
    42001 ttgcaaacaa aatggacaag atcggtgctg acttcttata ttcagtatca actttacatg
    42061 accgtttgca agcaaacgct cacccaatcc aattaccaat tggtgcggaa gacgacttca
    42121 caggaattat cgaccttgta acaatgaagg ctgaaatgta tacaaacgat cttggaacag
    42181 agatcgaaga aacagaaatt cctgaagaat accgcgaatt agctgaagaa tggcgcgaaa
    42241 aattggttga agctgtagct gaaactgatg aagaacttac tttgaaatac ttagaaggtg
    42301 aagaaatcac tgaagctgag ttaaaagaag gtatccgccg cgcaacagtt aacgtagaat
    42361 tctacccagt actatgtggt tcagcattta agaacaaagg ggttcaatta ctattggacg
    42421 ctgttcttga ttacttgcca tcaccgttag atatcccagc tatcaaaggg attgacccta
    42481 aaactgatga agaagtagaa cgtcctgcag acgacagcgc tcctttctca gctttagcat
    42541 ttaaagttat gactgaccca ttcgtaggtc gtttaacttt cttccgtgtg tactctggtg
    42601 ttttacaatc aggttcatac gtacaaaacg caacaaaagg caaacgtgaa cgtgttggtc
    42661 gtatcctaca gatgcatgcc aactctcgtt cagaaatctc tgaagtttac gctggagata
    42721 tcgctgctgc tgttggtttg aaagacacaa ctactgggga cacactatgt gatgaaaaga
    42781 gtctagttat tcttgaatcc atggaattcc cagaaccagt tatccaagtc gctattgaac
    42841 ctaaatcaaa agctgaccaa gataaaatgg gtgtggcttt acaaaaacta tctgaagaag
    42901 atcctacttt ccgtgctgaa acaaacgtgg aaacaggcga aacaatcatc gctggtatgg
    42961 gtgaacttca cttagatatc atcgttgacc gtatgcgtcg tgaattcaaa gttgaagcta
    43021 acgtaggtgc gcctcaagta tcttaccgtg aaactttccg tgctggcact caagccgaag
    43081 gtaagtttgt acgtcagtct ggtggtaaag gtcaatacgg tcacgtatgg attgaattta
    43141 caccaaatga agaaggtgct ggtttcgaat ttgaaaatgc gatcgtcggt ggggttgttc
    43201 ctcgtgaata cattccagct gttgaaactg gtttgaaaga tgctatggaa aatggtgtct
    43261 tagctggcta tccattagta gatatcaaag ctaaacttta cgatggttca taccatgatg
    43321 tcgattcaaa tgaaactgcc ttccgtgttg ctgcttctat ggcattacgt gcagcagcta
    43381 agaaagctaa tccagtaatc cttgaaccaa tcatggctgt agaagttgtt atccctgaag
    43441 attatttagg tgatgttatg ggtcacgtaa ctgctcgtcg tggacgtgtt gaaggtatgg
    43501 aagctcgcgc tggcggccaa caagttgttc gtgcgatggt tccacttgct gaaatgtttg
    43561 gttatgctac aactcttcgt tctgctactc aaggtcgtgg tacatttaca atgacatttg
    43621 accactacga agatgtacca aaatcagtac aagaagaaat cattaagaaa aacggcggaa
    43681 aagctgaata atttcttgaa accggatagt cctgcaaaga gattatccaa agagactata
    43741 tttttaatat agtttcgtgt aaaataatta tcgatgatat gggcatgtgg tgtaccttgt
    43801 gtacatcata tacctatcag acaaataaaa aatatattgt atttttagga ggacatttta
    43861 aaatggcaaa agaaaaattt gaccgttcta aaccacacgt taacattggt actatcggac
    43921 acgttgacca tggtaaaact acattaacag ctgcaatcac aactgtacta tctaagaaaa
    43981 acggcggcca agctatggct tacgatcaaa tcgatggtgc tccagaagaa cgcgaacgcg
    44041 gaatcacaat ctcaacagct cacgttgagt atgaaacaga tacacgccac tacgctcacg
    44101 ttgactgccc aggacacgcg gattacgtta aaaacatgat cactggtgct gctcaaatgg
    44161 acggagctat cttggtagtt tctgctgctg acggcccaat gcctcaaact cgtgaacaca
    44221 tcctattgtc tcgtcaagtt ggtgttcctt acatcgttgt attcttgaac aaagtagaca
    44281 tggttgatga cgaagaatta ctagaattag ttgaaatgga agttcgtgac ctattaacag
    44341 aatacgaatt ccctggtgac gatgttcctg tagttgctgg atcagctttg aaagctctag
    44401 aaggcgacgc ttcatacgaa gaaaaaattc ttgaattaat ggctgcagtt gacgaataca
    44461 tcccaactcc agaacgtgac aacgacaaac cattcatgat gccagttgaa gacgtgttct
    44521 caattactgg acgtggtact gttgctacag gtcgtgttga acgtggacaa gttcgcgttg
    44581 gtgacgaagt tgaagttgtt ggtattgctg aagaaacttc aaaaacaaca gttactggtg
    44641 ttgaaatgtt ccgtaaattg ttagactacg ctgaagctgg agacaacatt ggtgctttac
    44701 tacgtggtgt tgcacgtgaa gacatccaac gtggacaagt tttagctaaa ccaggtacaa
    44761 tcacacctca tacaaaattc tctgcagaag tatacgtgtt gacaaaagaa gaaggtggac
    44821 gtcatactcc attcttcact aactaccgtc cacaattcta cttccgtaca actgacgtaa
    44881 caggtgttgt tgaattacca gaaggaactg aaatggttat gcctggcgac aacgtaacaa
    44941 tggaagttga attaatccac ccaatcgcta tcgaaaacgg tactaaattc tcaatccgtg
    45001 aaggcggacg tacagttggt gccggtgtcg ttacagaaat caaagcttaa ttcacttaag
    45061 ttttaaagta aagcaacatt tattcggacg taatcgctcg gaacgaaggt cttaggatct
    45121 ttgttccgag cttttttatt tattgcagga tcaggaaaaa agacaggagt attataaata
    45181 gaagaaacac gaaataaaag agtcagtatt ttctaaatga aactttatga aaacaaaatg
    45241 ttttttacaa gaaaaagcct tgctttgtga gtttatatat attataatga caaaggtgcg
    45301 tttactttag tgaattgcac agacgtaatc agaatgattt cgtcggctaa gaaacgagag
    45361 gttgcgacac gcccggacgc tttgccacga acgagcgtga cggaaatttt cgtggagcta
    45421 tgtctacttt tcaaaatagg cgaaggaggg aacaagatgg caaaacaaaa aattcgtatc
    45481 cgtttaaaag cgtatgaaca ccgtatttta gatcaatcag cggataaaat cgtggaaact
    45541 gcaaaaagaa ctggagctag cgtgtctggt ccaattccat taccaactga acgcagtctt
    45601 tacacaatca tccgtgcgac tcataaatac aaagattcac gcgaacaatt cgaaatgcgt
    45661 acacacaaac gtcttatcga cattgtgaac ccaacaccaa aaactgttga tgctttgatg
    45721 aagctagact taccatctgg tgtaaacatc gaaatcaaat tataaaacta aaacaaaaat
    45781 aatggaggtg tactcatgac caaaggaatc ttagggaaaa aagtgggaat gacacaaatc
    45841 ttcactgaat ctggtgaatt aattccagta actgttgtag aagctactcc aaacgtagta
    45901 ctacaagtga aaacaatgga aacggatggc tacgaagcta ttcaagttgg ttaccaagat
    45961 atgcgtgaag ttttatcaaa caaacctgcg aaaggtcatg ttgcaaaagc aaacacggct
    46021 cctaagcgct tcattcgtga attcaaaaat gttgagctag gagaatatga agtaggaaaa
    46081 gaaattacag tagatgtttt ccaagcagga gacattattg atgtaacagg tactacgaaa
    46141 ggtaaaggat tccaaggggt tatcaaacgc cacggacaaa gccgcggacc aatggctcac
    46201 ggttctcgtt accatcgtcg tcctgggtca atgggtccag ttgcacctaa ccgtgtattt
    46261 aaaaacaaac gtcttgctgg ccgtatgggt ggcaaccgcg taactatcca aaacttggaa
    46321 gttgtacgtg tcgatgcaga aagaaatgtt atcttgatca aaggtaatgt tcctggagct
    46381 aaaaaatctt taattactat caaatcagct gtgaaagcta aataattgta ggaagaaagg
    46441 aggaactaag gaatgccgaa tgtagcatta ttcaaacaag atggaagcca aaacggtgaa
    46501 atcacactta acgaagaaat cttcggaatt gaacctaatg aaagtgttgt ttacgatgca
    46561 atcgtaatgc aacgtgcgtc attaagacaa ggaacacacg cggttaaaaa ccgtagcgct
    46621 gttcgcggcg gcggacgtaa accatggcgt caaaaaggaa caggtcgtgc acgtcaagga
    46681 tctatccgct caccacaatg gcgtggaggt ggcgtagtct ttggaccaac accacgttct
    46741 tacagctaca aacttcctaa aaaagttcgt cgtttagcaa tgaaatctgt attgtcagaa
    46801 aaagttgctg aaaacaactt ggtagcaatc gaaggcttga acttcgacgc accaaaaaca
    46861 aaagaattca aacaagttct tgctaactta tctatcgact ctaaagtatt ggttgttcta
    46921 gaaaaaggta acgactttgc tgctttatca gcacgtaact taccaaacgt ttctgtagta
    46981 acttctgata atgtgagtgt gctagacgtt gtatcaaata caaaagtgtt agcaacacaa
    47041 actgctctta ctcaaattga ggaggtgctt gcataatgaa cttactagac gtaatcaaac
    47101 gcccagtgat cactgaaaaa tcaatgcttg ccatggatga caagaaatac acttttgaag
    47161 tggacactcg cgcaaacaaa actttagtaa aacaagcggt agaagctgct tttgacgtta
    47221 aagtcaaaaa cgtgaacatc gtgaacgtac gtgcgaaatt caaacgtatg ggcaaatatg
    47281 caggatatac aaaaaaacgt cgcaaagcga tcgttacatt gactgaagat tcaaaagaaa
    47341 tccaattatt cgaagctgct gaataagcac taatagataa ctcattaaat taggagggaa
    47401 aaacgtggcg attaaaaagt acaaacctac ctcaaatggt cgtcgtaaca tgacaggttc
    47461 tgattttgct gaaatcacaa catcaacacc tgaaaaatca ttgttacaac cattgaaaaa
    47521 ccatgctgga cgtaacaaca acggccgcat cactgtacgt catcaaggtg gcggacacaa
    47581 acgccaatat cgcgtgatcg acttcaaacg taacaaagat aacgttgttg caactgttca
    47641 aacgatcgaa tatgatccaa accgttcagc taacatcgcg ttagtacact atgaagatgg
    47701 agtgaaagct tacatcttag cacctaaagg actagaagtg ggtatgaaac ttgtttcagg
    47761 tccagaagca gatatcaaag tcggaaatgc tcttccatta gaaaacattc cagttggtac
    47821 tgttatccac aacattgaaa tgaaacctgg taaaggtgga caattgatcc gttctgctgg
    47881 tacaagtgct caagtacttg gtaaagaagg caaatacgta ttgatccgct tgaactctgg
    47941 tgaagttcgt atgatcttag ctgcatgccg tgctacaatc ggttctgtag gtaacgaaca
    48001 acacgaatta atcaacatcg gtaaagctgg acgctctcgt tggatgcgta aacgcccaac
    48061 tgtacgtggt agcgtaatga accctaatga tcacccacac ggtggtggtg aaggtaaagc
    48121 tcctatcgga cgtaaagcac cagtttctcc ttggggtcaa ccagctcttg gttacaaaac
    48181 tcgtaacaaa aaagctaaat cagacaaact tattgttcgt cgtcgcaaaa ataaataatt
    48241 actttgacca tgtgtcgcac attttgagag gaggttcacc atgggtcgta gtttaaagaa
    48301 aggacctttt gtcgatgagc atttaatgaa aaaagttgaa gctcaagcag gggttgaaaa
    48361 gaaaaaagtt atcaaaactt ggtcacgccg ttcaacagtt ttcccaagtt ttgttggata
    48421 taccatcgca gtttatgatg gacgtaaaca tgtaccagtt tacatccaag aagacatggt
    48481 aggacacaaa ttaggtgaat ttgcacctac aagaacttat cgtggacatg ctgctgacga
    48541 taagaaaacg aaacgctaat ttgaggggag gataaatcca aatggcagaa caaatcacat
    48601 cagctaaagc aactgcaaaa acagttcgca cttcacctcg aaaagcccgt ttagtaattg
    48661 accttatcag aggtaaaagt gtcgcagatg caatttcaat cttgaaattc acaccaaaca
    48721 aatctgctgg aatcattgaa aaagtattga tgtcagcagt tgcgaatgca gaaaataact
    48781 ttgacttaga tgttgaaaac ttggtagtaa gcgaagcttt tgttaacgaa ggacctacaa
    48841 tgaaacgttt ccgtcctcgt gcaaaaggat cagcttctcc aatcaacaaa cgtacaagtc
    48901 atatcacggt agtcgtatca gaaaaataag gagggataat cagtgggtca aaaagtacat
    48961 ccaattggaa tgcgtgtagg catcatccgc gactgggatg ctaaatggta tgctgaaaaa
    49021 gagtatgctg agttcttaca cgaagattta agaatccgta aatttatttc aactaaactt
    49081 gctgatgctg ctgtatcaac agttgaaatc gaacgcgcaa caggtcgcgt aaacatttca
    49141 attcacacag ctaaaccagg tatggttatt ggtaaaggcg gttctgaagt cgaaagccta
    49201 agaaaagaat taaacaaatt aactggtaaa agaatccata tcaatatcgt ggaaatcaaa
    49261 aaaccagatc tagatgcaaa attagtaggt gaaggaatcg cacgtcaatt agaaaaccgt
    49321 gttgctttcc gtcgcgctca aaaacaagcc atccaacgct caatgcgtgc tggcgctcaa
    49381 gggatcaaaa ctcaagtatc tggtcgtcta aacggagcag atatcgctcg ttcagaagga
    49441 tactcagaag gaactgttcc tcttcatact ttacgtgcgg acattgatta cgcatgggaa
    49501 gaagcagata caacatacgg aaaactagga gttaaagtgt ggatctatcg tggagaaatt
    49561 cttccaacta aaaaaaacac tgagaaagga gggaaataat catgttagta cctaaacgtg
    49621 taaaacaccg tcgtgaattc cgcggaaaaa tgcgcgggga agctaaaggc ggaaaagaag
    49681 tagcattcgg tgaatacggt ttgcaagctg ttgattcaca ttggatcaca aaccgccaaa
    49741 tcgaagctgc tcgtatcgca atgactcgtt acatgaaacg tggtgggaaa gtatggatta
    49801 aaattttccc tcacaaatct tatactgcca aagcaattgg ggtacgtatg ggttctggta
    49861 aaggggcacc tgaaggatgg gttgcaccag taaaacgtgg taaaatcatg tttgaaatcg
    49921 caggcgttcc tgaagaagta gctcgtgaag cgttacgtct agcttctcac aaattaccaa
    49981 tgaaaactaa gatcgtaaaa cgtgaggaaa tgggtggtga atcgaatgaa ggttaaagaa
    50041 atcagagaat taaccactgc cgaaatgctt gatcaagaaa aacaattaaa agaagaattg
    50101 tttaatctta gattccaatt agcaacaggt caattagaaa acactgcacg tattaaagaa
    50161 gtacgtaaat cgattgcacg catcaaaact gtgttgcgtg aacaagctaa gtaattatgg
    50221 aaggaggcca ttaagcgtat gactgaagga agaaatcaac gcaaagttta tcaaggtcgc
    50281 gtggtttcag acaaaatgga taaaacaatt accgttgctg tcgaaacaaa gaaaaaccac
    50341 cctatctatg gcaaacgcat gaactattct aagaaataca aagtgcatga tgaaaacaac
    50401 acagcaaaag ttggcgacat cgtgagaatt atggaaactc gtccattatc agctacaaaa
    50461 cgtttccgtt tgttggaagt agtcgaagaa gcagttatta tctaataaca cgagattttc
    50521 ctatatagat tgaatgcaaa atctgaaagg aggatacaca acgtgatcca agcagaaagt
    50581 cgtttaaaag tcgctgataa ctcaggcgca cgtgaaattt tgacgatcaa agtccttggt
    50641 ggatctggtc gtaaaactgc taacatcggt gatgtgatcg ttgctacggt taaacaagca
    50701 acgccaggtg gggttgtcaa aaaaggtgac gtcgttaaag ccgttatcgt tcgaacaaaa
    50761 tcaggagctc gtcgtacaga cggttcatat attaaatttg atgaaaatgc tgcagtgatt
    50821 attcgtgatg ataaaagccc tcgtggaaca cgtatctttg ggccagttgc acgtgaacta
    50881 cgtgaaaaca acttcatgaa gatcgtttca ctagcaccag aagttttata atcttgagac
    50941 acttatcaaa ggaggtgcga aacagttatg tttgttaaaa aaggcgataa agttaaagtt
    51001 atcactggaa aagacaaaaa caaagaaggc gttgtactag cagcgttccc gaaaaaagat
    51061 aaagttattg tcgaaggtgt aaatgtcgtt aaaaaacacc aaaaaccttc tcaagctgct
    51121 cctcaaggtg gaattgttga gatggaagcg ccaattcatg tttctaacgt gatggtggtt
    51181 gactctactg gtgtagctgg ccgagttggc tacaaagaag tagacggcaa aaaagtccgt
    51241 gtttctaaaa aaaccggtga agttttagat aaataaaagt ttaaggaagg agggtcttac
    51301 tgaatgaacc gcctaaaaga aaaatatctt aaagaagtaa ctccatcatt gatggaaaaa
    51361 tttaactata gctctgttat gcaaacacct aaagttgaaa aaatcgtcat caacatgggt
    51421 gttggtgatg cggtatcaaa cgctaaaaac ttagataaag cagttgaaga attaactttg
    51481 atcactggtc aaaaaccaat gattacaaaa gctaaaaaat caatcgctgg attccgttta
    51541 cgtgaaggaa tgccaattgg tgcaaaagtt acattgcgcg gagaaagaat gtacgaattc
    51601 ttagacaaat tggtttctgt ttcactacca cgtgtacgtg acttccatgg tgtaagtaaa
    51661 aaagcctttg acggtcgtgg aaactatact ttaggtatta aagaacaatt gatcttccca
    51721 gaagtagatt atgatttagt agataaagta cgtggtatgg acatcgttat tgtaacaaca
    51781 gcaaactctg atgaagaatc tcgtgagttg ttgacacaat taggcatgcc attccaaaaa
    51841 taattaaagg aggcgaacta cgtggctaaa aaatcaatga ttgctaaaaa caaacgtcct
    51901 gcaaaatttt caacacaagc ttatactcgt tgcgaacgtt gcggacgtcc acattcagtt
    51961 tatcgtaagt tccatctttg ccgtatttgc ttccgcgaac ttgcctataa aggtcaaatt
    52021 cccggcgtga agaaagctag ctggtaaaaa gtaaattcgc ataagaagga ggtaaaagat
    52081 caatggtcat gacagatcca attgcagatt ttctaactcg tatccgtaac gcaaacatgg
    52141 ttaaacatga gtctttagaa gtacctgcat caaaaattaa acgtgacatc gctgaaatct
    52201 tgaaacgtga aggtttcgtt cgtgatgttg aatacatcga agatgacaaa caaggcgtga
    52261 tccgtgtttt cctaaaattt ggtaaaaacg gcgaacgtgt tatcacaaac ttaaaacgta
    52321 tctctaaacc aggtttgcgt gcttatgtca aagctgacga agtaccaaaa gtattgaacg
    52381 gtttaggtat cgcaatcatt tcgacttctg aaggtgttgt cactgataaa gaagctcgtg
    52441 aaaaaaatat cggcggcgaa gttatcgctt acgtatggta atcaagtaga atctacaagg
    52501 aggtgtcttt aagtgagccg tatcggaaat aaagtcgttg ttatccctga aggagtaaca
    52561 gttactcaag acggaaacaa cgttacagtt aaaggaccaa aaggtgaatt gactcgtact
    52621 ttttcagcag atatcaaaat gaatatcgaa ggaaacgaag taactttcac tcgtccaaac
    52681 gacagtaaag aaatgaaaac aattcatgga acaactcgtg cgaacttcaa caacatggtt
    52741 gtaggtgtca gcgaaggttt ccaaaaagcg ttagaactaa tcggggttgg gtaccgtgca
    52801 caattacaag gatcaaaact tgtattgaac gttggttact cacatccagt agaaatcaca
    52861 ccaccagctg gagtaactgt tgaagtacca gctaacacac aagtaattgt taaaggtgcg
    52921 aacaaagaag aagttggcga attagctgca aacattcgtg gaactcgtcc tccagaacct
    52981 tataaaggta aaggtattcg ttatgttggc gaatatgtac gccgtaaaga aggtaaaact
    53041 ggtaaataat cgtttagaaa aattgcatag aagccagcag caggaaaaag gcatttggct
    53101 gaagtttacc ttcaaaaaaa tacattttta cttgtgccgc agcttctatt tttcgaaacg
    53161 tatttactgc ttgaaatagt agtacaataa aaataaagag gtgacaattg tgattacaaa
    53221 accagataag aacaaaacac gtcaaaagag acatcgtcgt gtacgtaaca atatctctgg
    53281 tactgctgag cgcccacgct tgaacgtttt tcgttctaac aaaaacatct acgctcaaat
    53341 tattgatgac gtagcgggtg taacgctagc aagtgcctct accttggata aagaaatttc
    53401 aggtggaaca aaaacagaag cagcacaagc tgtcggtaaa ctagttgctg aacgtgcagc
    53461 tgaaaaaggc attaaagtag tagtctttga ccgtggtgga tacctttacc atggccgtgt
    53521 gcaagcttta gctgaagctg ctcgcgaaaa tggactagaa ttttagaaga aggaggaata
    53581 ccattcatgg tttatatcga tccaaaacat ttggaattag aagaccgcgt tgttgcaatt
    53641 aaccgtgtaa caaaagttgt taaaggtgga cgtcgtctac gttttgctgc tttagttgtt
    53701 gtcggtgaca aaaacggaca cgtagggttt ggtacaggta aagctcaaga agttcctgaa
    53761 gcaatccgta aagcaattga agatgcgaag aaaaacttag ttgaagtacc tatggttggt
    53821 tcaacaatcc cacacgaagt aatcggtgct tttagtggtg gtagaatcct aatgaaacct
    53881 gctgtagaag gttctggggt tgctgctggt ggaccagttc gtgccgtatt agaattagct
    53941 ggggtagcag atatcacttc taaatcttta ggctcaaata caccaatcaa cgttgttcgc
    54001 gcaacagttg aaggtttgaa acaattgaaa cgtgcagaag aagttgcaga acttcgcggc
    54061 aaatcagttg aagaattaat cggataagga ggacaacaat aatggctgaa ttaaaagtta
    54121 ctttaaaacg cagtattatc ggacgtcctc aaaaccaacg cgatacagtt aaagcgttag
    54181 gtcttacaaa gattaacagt tcagtggtta agccttctaa tgaagcaatt aaaggcatga
    54241 tcaatactgt ttctcaccta gtggacgttg aagaaattta atttagaaaa atcataacta
    54301 gaacattaag gaggtgccaa acctatgaaa cttcatgaat taaaacctgc tgaaggttca
    54361 cgccaagtac gtaaccgtgt tggtcgtggt acttcatctg gtaacggaaa aactgccgga
    54421 cgcggacaaa aaggacaaaa agctcgttca ggcggcggtg tgcgtttagg atttgaaggg
    54481 ggacaaacac cattgttccg tcgtttacca aaacgaggat tcacaaacgt gaaccgtaaa
    54541 gactatgcag tcattaatct tgatgtattg aaccgctttg aagacggagc tgaagtaact
    54601 cctgttgctt tagttgaagc tggaatcgtg aaaaacgaaa aagctggaat caaagtatta
    54661 gctaatgggg aactaaacaa gaaattaact gtgaaagcag caaaattctc taaagcagct
    54721 caagaagcaa ttgaagcggc tggtggatca atcgaggtga tctaatgttt agactattaa
    54781 aagatgcttt taaagtaaaa gacattagat cgaaaatcct atttactgta tttgtgttgt
    54841 ttgtatttcg cctaggtgca catattactg taccaggcgt aaatgctgct tcattgcaaa
    54901 acctttcttc tttgccattt ttcaacatgt tgaacttggt aagtggtagt gcgatgcaaa
    54961 acttttccat tttctcaatg ggggtttcac catacattac tgcttctatc gtcattcagc
    55021 ttcttcaaat ggacatcgtg cctcgatttg ttgaatggtc gaaacaaggg gaagttggtc
    55081 gtaaaaaact gaatcaagcg actcgatatc tgacattagt attggcattt atccaatctg
    55141 tcggtatcac tgctggattc aatcaatacg ctcaattagg tttcgtgaat aatcctaacg
    55201 tagcaactta cgtaacgatc ggtttgattc taacagccgg tacgatgttt gttacttggc
    55261 taggagaaca gatcacagaa aaaggtatcg gaaatggcgt atcaatgatc atttttgcag
    55321 gtatcatttc tagattgcct gaaagtatca gagaaattta tgaagattac tttataaata
    55381 ttgatccttc agacatatgg aaatctgcta tttttgtggc cattttacta gtagcggtct
    55441 tgttgatcgt gacattcgta acatttttcc aacaagctga acgcaagatc ccgatccaat
    55501 atacgaaacg cgtagctggc gcgcctacaa gcagctatct gccgttgaaa gtaaatgcag
    55561 ctggagttat tccagttatc tttgctagtt cgtttattgc tacgccaaat gctgttttac
    55621 aagcattgag cggtagattc ggttctgaaa actggtacaa agtgatgatg gagatcttta
    55681 actataatac agcacctggt gctacgattt atacgattct gatcgttgcc ttcactttct
    55741 tctacgcctt tgttcaagta aaccctgaga aattagcgga aaacttgcaa aagcaaggaa
    55801 gttatatacc aagcgtacgc cctggtaagg ggactgaaga gtatgtctca agattactga
    55861 tgcgtttaag tacagttggt gcagtcttcc ttggtttagt ggcattgctt ccaatcattg
    55921 ctcaaatggt ttgggatctt ccacaatcaa tcggattagg cggtacaagc ttactgatcg
    55981 ttatcggtgt agctttagaa actgcgaaac agttagaagg cttaatgctg aaacgtaaat
    56041 atgttggatt cattgagtaa gaaagcgtgt tgaggtttcc cctcaacaca cgttctcaca
    56101 catttattag gagggaaaac aatgaacctc attttaatgg ggctgccagg cgcaggtaaa
    56161 ggaacacaag ctgaaaaaat cattgatgct tatggtattc cgcacatctc aacaggagat
    56221 atgttccgcg cagcgatgca aaacgaaaca gctcttggcc tggaagcaaa gtcttatatg
    56281 gataaaggtg cattagttcc tgatgaagta acaaatggaa tcgtgaaaga gcgactagct
    56341 gaaccagata cagagaaagg cttcttatta gatggctttc cacgtacgct agatcaagca
    56401 aaagctctgg acgcaatgct aaaagatttg aataaaaaaa ttgatgctgt catcgatatc
    56461 catgttggtg aagaaatctt ggtcgaacgt ctagccggcc gatttatctg cagcaattgt
    56521 ggagcaactt accataaaat tttcaaccct acaaaagttg aagacacatg tgatcgttgc
    56581 ggtgggcatg aattctatca aagagaagat gataaacctg agacggttaa aaatcgtctg
    56641 gctatcaaca tcaaaaatag tgaaccaatc ttggcttatt ataaagaaca aggtttgcta
    56701 aacactattg atggtgctcg tgaaatcgat gctgtatttg cagatgtaca aaaaatcatt
    56761 gaaaaatagc acgattgccc gtagccgact tgcattatgc agttactctt gtcaagttca
    56821 ataaattatg atagaatatg tcagtccgac ttctagagaa tcctctagat gatccatttt
    56881 gagaattttg aggtgggaac tgcagtttcc gccattttat cgtgtgaaaa tgcgaaacaa
    56941 gtacaaggag gtactgtgcg tggcaaaaga agatatgatt gaagtcgaag gtacagtcgt
    57001 cgaaactttg ccgaatgcaa tgtttaaagt tgaactagaa aacggacacc aagttcttgc
    57061 tactgtttca ggtaaaattc gtatgcatta catccgtatc ttacctggtg acaaagtaac
    57121 ggttgaattg tctccatacg atcttaaccg tggccgtatt acttatcgct ttaaataatt
    57181 gtactccgta aaactacagg aggtattatc atgaaagtaa gaccatcagt aaaaccaatg
    57241 tgcgaacatt gtaaagtaat tcgtcgtaaa ggacgcgtta tggtgatttg cccagcaaat
    57301 ccaaaacata aacaacgtca aggataattg gaggtgctat aaatatggct cgtattgcag
    57361 gagtagatat tcctcgtgat aaacgtgtag tagtttcact tacttatatc tatggtattg
    57421 gtaacactac tgctaaaaag atcttagctg atgctggtgt atctgaagat gttcgcgttc
    57481 gtgaattaac gaatgaacaa acagatgcta tccgtgcaga agtagataaa ttaaaagttg
    57541 aaggggatct tcgtcgtgaa gtgaacttga acatcaaacg tttgatggaa atcggttctt
    57601 accgtggaat ccgtcatcgc cgtggattgc ctgttcgtgg acaaaacacg aaaaacaatg
    57661 cacgtactcg taaaggccca gctcgcgcta tcgctggcaa gaaaaaataa ttcttaagtg
    57721 aaggaggtta aaacttcatg gcagcaaaaa aagtgaatcg taaacgccgt gtgaagaaga
    57781 atatagaagc aggtattgca catatccatt ctacattcaa taacactatc atcatgatca
    57841 ctgatactca cggaaatgca ttagcgtggt catcagctgg ttcattaggt ttcaaaggaa
    57901 gcaaaaaatc aactcctttt gccgctcaaa tggcagcaga aactgctaca aaagcagcaa
    57961 tggaacacgg attaaagact gttgaagtaa cagttaaagg acctggttct ggacgtgaag
    58021 cagcaattcg ttcattacaa gctacaggtt tagaagtgac tgcaattcgt gacgtgactc
    58081 cagttcctca taatggtgcc cgccctccaa aacgccgtcg tgtttaatga gtgccgctca
    58141 ttttgagttt tatcagtaac gtcattgaac aagacacttt tcgttttgaa aggggtaaag
    58201 ataagaatga ttgagttcga aaaaccaaga atcgcaaaaa ttgatgaaga aaaagattat
    58261 ggcaagttca tcgttgaacc tcttgaaaga gggtatggga ctactttagg gaattcccta
    58321 cgtcgtattt tattatcttc tttgccaggt gctgccatca ctaatattca aatcgatggt
    58381 gtactgcatg aattctctac catcaaaggt gttagagaag acgtcactca aatcatcttg
    58441 aacattaaag gcttagcgct gaaaatgtat ggccaagaag aaaaaaccct tgaaatcgat
    58501 attaccggac ctgctacagt aactgctggc gatattatcg ttgatagtga tgtagaaatt
    58561 cttaacaaag atatgtacat ctgtagtgtc tctgaaggag ctacgttcca tgctcgctta
    58621 acagtgaaac ctggacgtgg ttatgttcaa gcagatgaaa ataaaaaaga agatatgcca
    58681 attggcgttc ttccagtcga ttcaatctac acacctgttc gtcgtgtgaa ctatcaagtt
    58741 gagaacacac gtgttggaca ccgtgaagat tttgataaat tgacgatgga gatttggaca
    58801 gatggttcaa ttgaaccgtt ggaagcaatg agtttgtctg cgaaaatcat gactgaacat
    58861 ttggatattt ttgtgaactt aactgatgaa gcaaaaaatg ctgaaatcat ggtcgaaaaa
    58921 gaagaaacac aaaaagaaaa aatgttggaa atgacgatcg aagagttaga cttgtctgtt
    58981 cgctcttaca attgtttgaa acgtgcagga atcaatactg tacaagaact tacaaataaa
    59041 tctgagcctg aaatgatcaa agtccgtaac ttaggacgta aatcattaga agaagttaaa
    59101 gcaaaattgc acgacttagg tttaggttta cgtaaagaag attaaaacct tttacgaagg
    59161 agggaaacca acgtgagtta tcgtaaacta ggacgcacat ctagccaacg taaagcgatg
    59221 ttgcgtgatt taacaactga tttattaatc aacgaacgta ttgtgactac tgaagctcgt
    59281 gcgaaagaag ttcgctcaac tgcagaaaaa atgatcactt taggtaaacg cggagatttg
    59341 catgcacgtc gtcaagctgc ggctttcgta cgtaatgaag ttgccagcgt gcgtgaagaa
    59401 aatgaggaca tcgtcattga atcagcttta caaaaactgt tcaacgatat tgcacctcgt
    59461 tacgcagaac gtcaaggcgg atacacacgc attttgaaga cagaaccaag acgcggtgat
    59521 ggtgcaccaa tggttgtctt agaacttgtc taaaatcaca ctaataacat cactttatag
    59581 atgattcttt tagagtgtta tgatgttgga agatattttt ccgagtctag ctcagcgctg
    59641 ctcctcaagc gaatatcgct tggggagtaa gcactcatca taaagtgttt ttttatacct
    59701 tcaattacct gtcatgacag atatgtttga aggcataaaa aaacatttta agcgagaaat
    59761 aattgaaaat tttgtctttt tttgatagtg ttagtaggaa ctttaggatg aagaaggagt
    59821 aatcatggag ccaattattg aattagaaaa gataaattat aaataccagc cagatgatct
    59881 tcgccccgca ttgaaagatg tctcctttac aattgataaa ggtgagtgga tcgctattat
    59941 tggacacaat ggttcaggaa aatcgacact tgccaaaacg atcaatggat tgcttctgcc
    60001 agaatcaggg atcgtgaaag tgggaaatca aatattggat gaagaaaata tttggacgat
    60061 ccgacagatg gttggaatgg tttttcaaaa tccagacaat cagtttgttg gttcgacagt
    60121 agaagatgac gttgcttttg gtcttgaaaa tcaagggatt cccagagaag aaatgctagt
    60181 gcgtgtgaaa gatgcacttg agaaagttag aatggctgag tttgcttcac gcgagcctgc
    60241 tcgtttatca ggagggcaaa aacaacgtgt tgccattgcc ggcgtggttg ccttacgacc
    60301 tgatatcatt attttagatg aagcaaccag tatgctagat ccagaaggtc gtgaagaagt
    60361 gatctctacg atcaagaaga tcaaagaaga aagtcaattg accgttatat ctatcactca
    60421 tgatattgat gaagcagcta atgccaatcg gatactagtg atgagacaag gcgaacttgt
    60481 ccgtgaaggt acaccaaaag aaattttttc tgctggtcct gaactgatcg acttaggtct
    60541 agatctacca tttcctgaaa aactgaagag cgcactgaaa gaacgtgggg ttgatgtgcc
    60601 cagtgaatat atgacagaag aaaggatggt ggattggtta tggacatccg ttttgaacaa
    60661 gtagacttca cttatcagcc gaatacacca tttgaacaaa gagccttatt tgacatcaat
    60721 atgacgatca aagaaaacag ttatactgca ttagtagggc ataccggaag cgggaaatca
    60781 accttacttc agcatttgaa tgcgcttgtt aagccaacaa gcggtacagt ccatattgga
    60841 gaacgagata ttcagccgga tacggataat aagaatttaa agcccatccg aaaaaaagtt
    60901 ggcatcgtct ttcagtttcc agaagcacag ctattcgaag aaacggtagc aaaagacatt
    60961 gctttcggtc ctaaaaactt tggtgtcagc gaagaagaag cactagtcct agcaaaagaa
    61021 acattagaac aagttgggct ggatgaaagc tatttggaac gttcgccatt tgaactttca
    61081 ggagggcaaa tgcgtagagt agcaatcgct ggtgtgcttg ccatgaggcc ggaagtactt
    61141 gtattggatg aaccaacggc aggacttgat ccccagggta gaaaagaaat gatggagatg
    61201 ttttggcgtc ttcataagga acagcagatc actattgtat tagttacgca tttaatggat
    61261 gatgtagcca attttgcaga ttatgtctat gtgttggaaa agggacgaat cgttaatagc
    61321 ggggagccgc aagaggtctt tcaaaacatt gaatggctaa aagaaaaaca actaggcgta
    61381 cctactgcca ccgagtttgc agaagaatta atggctaaag ggatgaactt tgctaccttg
    61441 cctttgacag ctgaagaatt agcagacgct atcgtccatt atgctggagg aaatgcccat
    61501 gatgaataaa ttgatatttg gtcgttatat gcctggagat tccttgatcc atcgattgga
    61561 tccccgagct aaattattag ccagttttta ttttatcgga atcatttttt tagcaaataa
    61621 ttgggaaacg taccttttgt tggcattttt cacattactt tctgtcttct tgtcaaaagt
    61681 agatttgaaa ttttttatcc gaggcattcg cccattgatc tggctaatcc tcttcactgt
    61741 agctttgcag gtcttgttta caagtggcgg agaaacttat tggagttggg gtgttttcca
    61801 tatcactgaa tttggtgtga tgaacggcct ctttattttc tgtcgattcg tgttgatcat
    61861 ttttatgtcg actctactca cgcttactac accgcctttg gaattatcgg atgcgattga
    61921 gtacttgctt cgtccattga aagccgtacg gttcccagtc cacgaaattt cattgatgct
    61981 gtcgattgct cttcgttttg tgcctacatt gatggacgaa acagaaaaga tcatgaacgc
    62041 tcagcgcgct cgcggtgttg attttggtga aggaaaccta gttcagaaaa tgaaagcagt
    62101 cattcctttg ctgattcctt tatttgtcag cagcttcaac cgtgcggaag atcttgcgac
    62161 tgctatggaa gcaagaggct acaaaggtgg ggaaggaaga acgaaatatc gtgtattgca
    62221 ttggcataat agagacacga tggttatcat tgcttttgta ttattaactg tgctactgat
    62281 ctttttgaga ggataaacgt tcagtgggcc gctatctatt cttttcttgc tgatctgttt
    62341 gcaagaaagg atgcgagcag tgtatgacaa gaaatattag ggtctgtggc agactaaggt
    62401 cacaggcctc tttcttttat ctgaaaagaa agaacgaaaa aattttataa aaaaatcatt
    62461 attccagata accttttagg aacaaaggag agaaaaacat ggtacgttac aaagcaatca
    62521 ttgcatacga cggaacaaat tttaatggat tccaaaaaca gccaaatggg cgtactgttc
    62581 aagaagaagt agaaaaaaca ttacaaaaaa tggcaaatgg aaaagaaatc acggtctttg
    62641 gttcgggaag gacagatgcc ggagtacatg cgattgggca agtaatccat tttgattatc
    62701 cagaggagcg tccgttagaa cgaatgcgtt tcgcgctaga tacccagtca ccagaggata
    62761 ttgctgtaag acaagtagaa attgtttcag aagacttcca cgcaagatat ttggtcaaag
    62821 aaaagacgta tcaatttcga gtggatatag ggaaaccacg cagtcctttt cgacgccatt
    62881 atgcaagtta ttttccttac cctcttgatt tggaaaaaat tcagcgggcg cttcctgatt
    62941 tattagggac tcacgatttc acctcttttt gtgcatcagg aagctcgata gaggataaag
    63001 tacgtacgat ctacgaggct aagatggagg taaatgaaac aaaagacgaa ttgttgttta
    63061 catttagggg aaatggcttc ttgtataaga tgatacggat cttagtagga acgcttttga
    63121 aaattggcaa tggccgattg cctgaagata gtatcccaga tattattgcg aaaaaagatc
    63181 gtaatgcagc aggacctaca gcacatcctg aaggacttta tttgtatgaa gtgaaatatg
    63241 aataagaggt tgtgacagaa gtggtcagct tcaagaaata agaaggaatt tacgaaaatt
    63301 acttctcaaa attttgcaaa atttcggctt attttcgaag gagctacttc tggaacaccg
    63361 tgaatcagag gttgtgaaaa agctgccctg catcaagtaa taagaacaac caaacaaaaa
    63421 tagctcgtca tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac
    63481 cgtttaagga agaggttgtg aaatcagcgt tttgacctga gtattagaga aacaaacgga
    63541 aaaatagttc ctcatatttt gccgtttgtt gagctaatac cgaaggtcag ctgattgaac
    63601 accgtttaag aaagaggtcg tgagcctgtc atttaactct gagtcacaag taacaatttc
    63661 aaaaaacagc ttctcatctt tgatgaatat cgtgacttgt ggacgaggcg ttgataagtg
    63721 aatcatcttt ttgttaccta ttttttctgt aaccaaaaaa cgatggaacg ttgttttgat
    63781 tgcccttaat gaggacagtc ggaacaggtt tgtcattttt atatttaaat ggcacaaatt
    63841 tttctaatta tttgtcattt agcgctgaaa atttttgcta tttatctaaa aaagtgataa
    63901 ctacttaaga tgaagcagaa tatttacgcc atacggcata caaccgcgaa aaatataaaa
    63961 aaaagaaaac gattgaacgc gtttttgcag tcgcaaagga aaagcatgga atgcgctaca
    64021 tcaagtatcg agacttggaa aacaatcagg tgtatacgct gattgttttt gctgcaatga
    64081 atctgaaaaa gttgtccact tggcagttgg aacatatgtc ttcatatctt ttattataca
    64141 aaaatagcaa aagcaccagt ctacactctt agtaaagagg aaactgatcc ttttgtctac
    64201 attcttaaag tacttaaact aagtacttta ttttactctt taggattgat acagtagcat
    64261 ctatcccttt attaataatc ttttattttt ttaaataaag atgttgtctc catcttcagg
    64321 cactaaaact ttttcggaaa ttcctttttc tttattgaaa gactttaatt cttctcttga
    64381 cagtccccaa tggtttacag cttccatatg aacggcaata attgtggaat ctggtattgc
    64441 tttatgcaat tcatataaat cgtctttccc cataataaga gattctccaa ctaaaaaatg
    64501 attatctcca gcatttgcta ctatgatttc tggattatac tgatcaatag tttgtttaac
    64561 agcttcatac caaactgtat ctcctgtaaa gtatagtgtc ttctcagtcg aatgtttgaa
    64621 aacaataccg caaacattac ctacataatt caagatttct cctctgccat gtttgccttt
    64681 tgttttaacc aattgtatag attcaaaaac tgtgtcttct cgtagtacct ccacattttt
    64741 aaaaccatat agtcgaattt cattcgcatc attttcatct tgcacaaata attttatttc
    64801 tttaggtagg acctctttcg ctatatcgtc ccaatgatct agatgtaaat gtgttacaat
    64861 aacagcatca ataccgttga taatatcttc gactggcatt ggcaaatcaa ctagaggatt
    64921 catttgatct tttcttggtg atggaaaagg acctttcgaa ataaatggtg gaagtgttcc
    64981 tttttccgct aacattggat caattaaaaa ttttttacct ccatagtcta agatagctgt
    65041 tgcattacga atgtgatgta tattcataaa cgttgtctcc tttttgttga tatgaaatat
    65101 ttttagtata attcaattcc atacaataaa taaaccgaac aaaaaaagtc ttttactatt
    65161 tttacttaat ttatgaaagg ttgtttacca tgttagacca aacagatata aatatcattg
    65221 aggaactatc aaaaaatagt cgaatcacaa tgaaagaact tggagaaaaa gtacatttga
    65281 ctggacccgc tgtctcggcg agagtgacaa aactagagga aagtggagtt attgaaaatt
    65341 atacagtaaa attaaatatg gaaaagctag ggttttcgat tcacgctttt ttaattataa
    65401 ttacgcaaaa tttaaatcat aaaccctatt tatcatttat taaaaatcat gaaaaattct
    65461 tgattagaaa ttataagatt agtggagggg gctgttatct tttggaatgt agatttactt
    65521 caaaaaaatc aatgaacagc tttttggaac agcttaatga atatgcgaat tataaattat
    65581 ctattgttat agattaataa taaaactagc agatggtaaa aatactaata tgcgagacac
    65641 acattcaacc ataactatat aacttatcaa tttaaaacta aactgatgga aacaagtaag
    65701 gcaaaaacat ttatattttg ccctacttat ttttttaatt aaattcattg atgattcata
    65761 tttagaaaat tctatgaagg ccaaatgagt atcctgtcac ttttttagat aaatagcaaa
    65821 aattttcagc gttaaatgac aaataattag aaatatttgt gccatttaaa tataaaaatg
    65881 acaccaagta tatcatgaac gcacaaaaag accaacaggg tataccctgt tggtctaaa
//
LOCUS       DANJMI010000011        64887 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000011 DANJMI010000000
VERSION     DANJMI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.011"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(<1..1421)
                     /locus_tag="PWU81_000856"
     CDS             complement(<1..1421)
                     /locus_tag="PWU81_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDL1109582.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
     gene            1673..2854
                     /locus_tag="PWU81_000857"
     CDS             1673..2854
                     /locus_tag="PWU81_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL1109583.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(2914..4542)
                     /gene="treC"
                     /locus_tag="PWU81_000858"
     CDS             complement(2914..4542)
                     /gene="treC"
                     /locus_tag="PWU81_000858"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDL1109584.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(4993..7704)
                     /locus_tag="PWU81_000859"
     CDS             complement(4993..7704)
                     /locus_tag="PWU81_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1109585.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7714..8415)
                     /locus_tag="PWU81_000860"
     CDS             complement(7714..8415)
                     /locus_tag="PWU81_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1109586.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8601..9200
                     /locus_tag="PWU81_000861"
     CDS             8601..9200
                     /locus_tag="PWU81_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL1109587.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9281..10294
                     /locus_tag="PWU81_000862"
     CDS             9281..10294
                     /locus_tag="PWU81_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL1109588.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10402..11319
                     /gene="rbsK"
                     /locus_tag="PWU81_000863"
     CDS             10402..11319
                     /gene="rbsK"
                     /locus_tag="PWU81_000863"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL1109589.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11347..11742
                     /gene="rbsD"
                     /locus_tag="PWU81_000864"
     CDS             11347..11742
                     /gene="rbsD"
                     /locus_tag="PWU81_000864"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL1109590.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11742..13115
                     /locus_tag="PWU81_000865"
     CDS             11742..13115
                     /locus_tag="PWU81_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1109591.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13472..15616
                     /locus_tag="PWU81_000866"
     CDS             13472..15616
                     /locus_tag="PWU81_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL1109592.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(15668..16708)
                     /locus_tag="PWU81_000867"
     CDS             complement(15668..16708)
                     /locus_tag="PWU81_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL1109593.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(16985..18787)
                     /locus_tag="PWU81_000868"
     CDS             complement(16985..18787)
                     /locus_tag="PWU81_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1109594.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18801..19553)
                     /locus_tag="PWU81_000869"
     CDS             complement(18801..19553)
                     /locus_tag="PWU81_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1109595.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19562..19924)
                     /locus_tag="PWU81_000870"
     CDS             complement(19562..19924)
                     /locus_tag="PWU81_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL1109596.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20100..20972)
                     /locus_tag="PWU81_000871"
     CDS             complement(20100..20972)
                     /locus_tag="PWU81_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL1109597.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21023..21511)
                     /locus_tag="PWU81_000872"
     CDS             complement(21023..21511)
                     /locus_tag="PWU81_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDL1109598.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(21649..22440)
                     /locus_tag="PWU81_000873"
     CDS             complement(21649..22440)
                     /locus_tag="PWU81_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDL1109599.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22495..23103)
                     /locus_tag="PWU81_000874"
     CDS             complement(22495..23103)
                     /locus_tag="PWU81_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDL1109600.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23096..23413)
                     /locus_tag="PWU81_000875"
     CDS             complement(23096..23413)
                     /locus_tag="PWU81_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL1109601.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23569..24297)
                     /locus_tag="PWU81_000876"
     CDS             complement(23569..24297)
                     /locus_tag="PWU81_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL1109602.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24456..25337
                     /locus_tag="PWU81_000877"
     CDS             24456..25337
                     /locus_tag="PWU81_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL1109603.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25574..26617)
                     /locus_tag="PWU81_000878"
     CDS             complement(25574..26617)
                     /locus_tag="PWU81_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL1109604.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26620..27288)
                     /locus_tag="PWU81_000879"
     CDS             complement(26620..27288)
                     /locus_tag="PWU81_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1109605.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27430..29766
                     /locus_tag="PWU81_000880"
     CDS             27430..29766
                     /locus_tag="PWU81_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1109606.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29881..30192)
                     /locus_tag="PWU81_000881"
     CDS             complement(29881..30192)
                     /locus_tag="PWU81_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109607.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30373..31848
                     /locus_tag="PWU81_000882"
     CDS             30373..31848
                     /locus_tag="PWU81_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDL1109608.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31895..32479
                     /locus_tag="PWU81_000883"
     CDS             31895..32479
                     /locus_tag="PWU81_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL1109609.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            32481..32864
                     /locus_tag="PWU81_000884"
     CDS             32481..32864
                     /locus_tag="PWU81_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HDL1109610.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(33181..33465)
                     /locus_tag="PWU81_000885"
     CDS             complement(33181..33465)
                     /locus_tag="PWU81_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HDL1109611.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33817..34527)
                     /locus_tag="PWU81_000886"
     CDS             complement(33817..34527)
                     /locus_tag="PWU81_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HDL1109612.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34524..35996)
                     /locus_tag="PWU81_000887"
     CDS             complement(34524..35996)
                     /locus_tag="PWU81_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL1109613.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36134..36607
                     /locus_tag="PWU81_000888"
     CDS             36134..36607
                     /locus_tag="PWU81_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL1109614.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36760..37311)
                     /locus_tag="PWU81_000889"
     CDS             complement(36760..37311)
                     /locus_tag="PWU81_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL1109615.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37416..37946
                     /locus_tag="PWU81_000890"
     CDS             37416..37946
                     /locus_tag="PWU81_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HDL1109616.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38429..38872)
                     /locus_tag="PWU81_000891"
     CDS             complement(38429..38872)
                     /locus_tag="PWU81_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1109617.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38888..40048)
                     /locus_tag="PWU81_000892"
     CDS             complement(38888..40048)
                     /locus_tag="PWU81_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL1109618.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40555..42255
                     /locus_tag="PWU81_000893"
     CDS             40555..42255
                     /locus_tag="PWU81_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109619.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42357..43190)
                     /locus_tag="PWU81_000894"
     CDS             complement(42357..43190)
                     /locus_tag="PWU81_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDL1109620.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43494..45659)
                     /locus_tag="PWU81_000895"
     CDS             complement(43494..45659)
                     /locus_tag="PWU81_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1109621.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45919..48213
                     /locus_tag="PWU81_000896"
     CDS             45919..48213
                     /locus_tag="PWU81_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL1109622.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48206..48871
                     /gene="pgmB"
                     /locus_tag="PWU81_000897"
     CDS             48206..48871
                     /gene="pgmB"
                     /locus_tag="PWU81_000897"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL1109623.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            48864..49871
                     /locus_tag="PWU81_000898"
     CDS             48864..49871
                     /locus_tag="PWU81_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL1109624.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49893..50912
                     /locus_tag="PWU81_000899"
     CDS             49893..50912
                     /locus_tag="PWU81_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1109625.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51193..52560)
                     /gene="nhaC"
                     /locus_tag="PWU81_000900"
     CDS             complement(51193..52560)
                     /gene="nhaC"
                     /locus_tag="PWU81_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL1109626.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52669..54090)
                     /locus_tag="PWU81_000901"
     CDS             complement(52669..54090)
                     /locus_tag="PWU81_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1109627.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54333..56210)
                     /gene="tdc"
                     /locus_tag="PWU81_000902"
     CDS             complement(54333..56210)
                     /gene="tdc"
                     /locus_tag="PWU81_000902"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL1109628.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56501..57757)
                     /locus_tag="PWU81_000903"
     CDS             complement(56501..57757)
                     /locus_tag="PWU81_000903"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL1109629.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57869..58074)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58337..59638
                     /locus_tag="PWU81_000904"
     CDS             58337..59638
                     /locus_tag="PWU81_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL1109630.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59707..60306)
                     /locus_tag="PWU81_000905"
     CDS             complement(59707..60306)
                     /locus_tag="PWU81_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1109631.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60560..61441
                     /locus_tag="PWU81_000906"
     CDS             60560..61441
                     /locus_tag="PWU81_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1109632.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61454..63064
                     /locus_tag="PWU81_000907"
     CDS             61454..63064
                     /locus_tag="PWU81_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HDL1109633.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63141..63800)
                     /locus_tag="PWU81_000908"
     CDS             complement(63141..63800)
                     /locus_tag="PWU81_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL1109634.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64083..64748)
                     /locus_tag="PWU81_000909"
     CDS             complement(64083..64748)
                     /locus_tag="PWU81_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL1109635.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT        19457 a        13218 c        11704 g        20508 t
ORIGIN      
        1 tttcctgcat ataaatgaaa atgttcggag tcagccggtg caatcgtgtg atcactgaat
       61 tgaatatatc taaaagcgcc actttcctta tcttctgctt cgaatagata ccttacgcca
      121 cggtttcctg cctcgtaggt caagatttct ttccctacat aacgataatt tgaacgtttc
      181 gttgttccat tcttaaacgt aaatgccatc tctttctcac tgatcttgat tgaagcaata
      241 tctgtttgat agcccttcgt ataatattct ttatattctt gtgctgtctt atcctgattc
      301 aatttttctt tatattcaaa aacaggatca aaagtcccat cttctacata cgtatctact
      361 gtttgccaag ttcctgccca atctgaaaga gagcgatctt gtacatcctc gtcttcaaaa
      421 taaccgttgt agacgctctt ttcttttttg ggggtcaaag tatcttctag cgggttttct
      481 gtctctgtcg tttttttcaa cgcttcgagg tttgcttcca tgatagaaag atagttttct
      541 ccattttcta tttgttcatt cgtcaatcct tccaaaggat tcaatacttc taaagataca
      601 cctgcttcgt ttgctaacgt tctggcaata ctatctttgg cgttttcttc aaaataaata
      661 tagttgattc catgttcttt aacaaagtgt ttcagttcag caattctcgc cgcagacggt
      721 tcttcactag aagaaagacc agcaatcgat acttgattca atccatattc taacgctaga
      781 tagttgaatg cagcatgttg cgtgacaaaa gatctttgtt ttgcttgctt aaaggtttct
      841 tgataaagct gatggagtgc ttgaagtttc tcggtatagg ctgctgcatt ttttgtccat
      901 acttcctgtt tttctgggta agcttcaatc agctgatcac gtatgtttgc tacctgcttg
      961 attgctaaac ttggagctag ccatacatgt gggtctagct catgtgtatg gccttcttcc
     1021 ccgtgttcat ggtcatgatc atgtccttct tcacttcccg gaagtaaaag cataccttct
     1081 gtcgctttta tcgtatggac attgccttct tgtaatgttt tttggattgc ggggacccat
     1141 gtttccatgt tttcatcatt ataaacaaag atatctgtat cttggatctt tgccatgtct
     1201 ttagcagaag gttcataatc gtgggaatca gtccctgctg gaatcaataa ctctacttct
     1261 ccttcatccc cgacgattgc ttttgtaaaa tcatacatgg gataaaaggt cgtcatcacg
     1321 ctcaattttt ctttactgtc ctcggctttc ccacttgccc cgcaagcagt taacatacct
     1381 gctaaaacca gcatggccaa tatacctatc aatcgtttca tttatgacac tcctcttctc
     1441 atcttctttc atttgaatgt tccgattcgc ttttcgcaaa ccgtaatagt tacgttttag
     1501 caacaggatt taatttatca tatggttaaa agaactgtca agcgttttct tattttaatt
     1561 gatgagaaag tttatatgaa tgattgacga tatttttgca atcaacctct atcattttcc
     1621 ttttataccc gcttttgtct ataatgaagt tatagataaa ggaggaaaaa gagtgaaaaa
     1681 agaaacagat acaacgaatc gtttgatccg ctttttagga ggaaaaacct cttactatgt
     1741 tttaggaata gttatcttac ttgcgctgat catttatttt ttccatcaga tctcgtttat
     1801 attcaagcct gtcggaatca ttatcgctac tatcttgccc ccattattgt ttgcactgat
     1861 cttgtattat cttttggaac cagtcatcaa acgtttgagc aagcaccttt cgaggaactt
     1921 gtcagtcatt atcgtttatg tgattatttt atttttattg gggcttggaa gcatatggct
     1981 tattcctttt cttgaagaac aggcaaagac attgatcagt agtttgccag atttgtttca
     2041 agattttcaa gagagtctcc gacaattttt ggagaaaaca ccttttgccg attcatttaa
     2101 tcaatttttc gcttctattg acgacgtgac gtctgatata gctggattta ttggaaatta
     2161 ttgggaaagt ggcgcacaac ggatcagcaa cattttttca actgttacag ctatttttat
     2221 tactttattt actggtccaa ttgtcgcttt tttccttttg agagatcctc aaaaatttta
     2281 tcagtcagtt ttagcgattg tcccaccagc atttcgtaca gattttaaaa atttgacaaa
     2341 gattgctaat caacaattgg gttcatttct aaaggggcaa atcattgctt catttatcct
     2401 tggagcagtc tactgggttt gtttcttact gatcggatta gaatttgcta gtgttttagc
     2461 tatttctgct gggttgttat gtatcattcc atatattggg ccgtttatcg ccttctttcc
     2521 aggtttgatc attgcattcc aagactctac ttttatggct gtcaaatttg tcattgtctg
     2581 gtttgccgta caacttctcc acggtgactt ggtgattcct cgagtaatgg gtgatcgttt
     2641 acagatccat ccgattacga tcttaatcgt tttacttgtc atgggtgatc taatgggaat
     2701 tgtgggcgtt atttttggta ttcctatcta tacgttagtc aaattactcg ttacttttat
     2761 tttccgtaaa ttcaaacaac gctacaataa attttatggc gataaaggag agtatgaaaa
     2821 ctcttcattt tctgaagaag attattttaa ataaaacaca aaacgtcgat cttacgagaa
     2881 tagctcgtag gaacgacgtt ttgtgttttt gttttaattt ttcagcaaag caattgcctg
     2941 gtatggtttt agctccagac tttgatgaag aacagtatcg gaataattat cgataagaat
     3001 attggcttct gtgaatcctt ctgggatggc gatccttgta tctttatcat agaaatttgt
     3061 taatacaagc agctgctgtc cttcaaattg gcgaagaaag ccataaactt ccggatgatt
     3121 tgcttcataa gcatgataat ctccttgtgc aacgattgga tatgtttttc tcagccgaat
     3181 caatttcttg taataggaca aaatagaatc ttcagaagat agttcttttt ctacattaat
     3241 ctcttctagc ttgcctccaa cttttaacca aggtttccct gtagtaaatc ctgcttgttc
     3301 tgtgtctgac cattgcatgg gtgttcgaga gttatcgcga gattttgctt tgatccgacg
     3361 aaacgcttct ttttctgtga atccttgttc aaggagtaac ttataagcat tcaaactttc
     3421 tacgtcaata tagtcggaaa tcgatgaata gtctgggtcg atcatgccga tttcttctcc
     3481 catataaata tagggcgtcc cacggttcaa atggatcatg gttgctagca tctctgctcc
     3541 ttgttttcga tactgatcga tagctacaaa tcgattgagc gcacgtggct gatcatgatt
     3601 attaaaaaat agagcattcc agccgttacc ctcactcatc ttttctcccc atgtatgaaa
     3661 tagtcgcttt aattctgcaa aatcaaatgg aggagtagac catttattcc cattttcata
     3721 atcgactttc aaatgatgga aattaaagac cattgataat tcttctctac taggttctgt
     3781 atacaagata caattttcga tcgtcgtaga gctcatttca ccgactgtga tgatagtgga
     3841 atccgtccca aacgttgctt ggttcaattc ttttagatac gtatgggtga ttggtttgtc
     3901 cgtatattca ggtttgccat cattttcttt attatttttc aattgttcgt ctttaccgat
     3961 aacattgata acatcgaaac ggaatccttt tacccctttt tccatccaaa aacgaacaac
     4021 tttgaatagt tcctctctta ctgcttggtt ccgccagttc aagtcagctt gtgtccgatc
     4081 atataagtgc aaataatatt tccccgtatc tccaaatggc gaccaagcac ttccaccaaa
     4141 ttttgatacc caatccgttg gctcttctct taaaatgaag aagtcctgat aatgctggtc
     4201 ccctgctagt gcttttttga accattcatg ttctgtcgaa acgtgattca gcaccatatc
     4261 gagcatgatt tctatccggt atttctttgc ttcggctatt agctgttcga attcctccat
     4321 cgtaccaaag ataggatcga tggctgtgta atcagagata tcataaccgt tgtctttttg
     4381 agggcttgga taaatagggt ttagccaaat catgtcgatg cctagatcat tcagatacgg
     4441 gagtttttct ttgattcctt ttaaatctcc aattccatca ccattactgt ctttgtaaga
     4501 tttgggatag atttggtaaa tgactttgtc tttaaatgtc atggatattt acctccattc
     4561 aactgattta ttacctacca caacttatat atacaagtta tcatttatta ttataactga
     4621 accgaaagcg tttgtaaaat ttatctttat acattctaag aggtctgttg cccaataaaa
     4681 aacaagagat cagacatgta tttgtctatc ctcttgttta aaatagcaaa atattcttgt
     4741 ttcttctata aatggtgttc aaaagctttt atctacggca atcagtcttc tcaaatgtaa
     4801 aacatgagga gctaatttta tttgagtatt ctttttgctc gatgcaggac ggctttttta
     4861 caacctctga ttcatggtgt tcaatcagct gtccttcggt attagctcaa aaaacggcaa
     4921 aatatgaaaa actattttgc cgtttgtttc tctaatactc aggtcaaacc gctgatttca
     4981 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5041 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5101 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5161 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5221 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5281 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5341 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5401 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5461 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5521 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5581 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5641 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5701 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5761 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5821 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5881 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     5941 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6001 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6061 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6121 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6181 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6241 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6301 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6361 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6421 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6481 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6541 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6601 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6661 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6721 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6781 ttgagataat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6841 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6901 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     6961 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7021 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7081 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7141 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7201 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7261 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7321 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7381 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7441 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7501 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7561 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7621 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7681 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7741 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7801 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7861 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7921 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     7981 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8041 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8101 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8161 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8221 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8281 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8341 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8401 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8461 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8521 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8581 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8641 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8701 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8761 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8821 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8881 aggattcttt gaaagataca gataatatgt tgactatcac aaaatacaat ccaagtgctt
     8941 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9001 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9061 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9121 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9181 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9241 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9301 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9361 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9421 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9481 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9541 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9601 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9661 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9721 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9781 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9841 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9901 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
     9961 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10021 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10081 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10141 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10201 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10261 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10321 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10381 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10441 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10501 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10561 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10621 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10681 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10741 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10801 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10861 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10921 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    10981 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11041 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11101 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11161 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11221 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11281 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11341 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11401 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11461 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11521 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11581 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11641 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11701 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11761 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11821 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11881 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    11941 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12001 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12061 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12121 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12181 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12241 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12301 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12361 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12421 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12481 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12541 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12601 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12661 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12721 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12781 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12841 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12901 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    12961 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13021 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13081 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13141 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13201 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13261 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13321 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13381 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13441 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13501 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13561 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13621 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13681 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13741 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13801 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13861 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13921 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    13981 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14041 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14101 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14161 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14221 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14281 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14341 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14401 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14461 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14521 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14581 ccaacctggc gacctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14641 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14701 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14761 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14821 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14881 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    14941 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15001 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15061 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15121 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15181 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15241 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15301 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15361 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15421 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15481 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15541 agctgcaact gttcctgtac taaccttgtt aaggttagac aaatcattct ggcttgatct
    15601 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15661 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15721 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15781 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15841 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15901 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    15961 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16021 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16081 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16141 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16201 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16261 gcgtataccg tacttctaca tctaaagttc caggaaatcc gttttccata tctgggctag
    16321 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16381 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16441 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16501 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16561 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16621 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16681 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16741 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16801 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16861 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16921 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    16981 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17041 agatgaaata caatacgaag aatatcgtaa acggaaccca aatatctttg tatggatttt
    17101 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17161 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17221 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17281 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17341 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17401 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17461 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17521 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17581 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17641 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17701 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17761 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17821 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17881 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    17941 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18001 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18061 tgaaataagt tcctaatacg atcgtaacta aggctacccc tataccaaag acttgatagg
    18121 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18181 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18241 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18301 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18361 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18421 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18481 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18541 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18601 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18661 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18721 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18781 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18841 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18901 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    18961 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19021 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19081 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19141 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19201 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19261 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19321 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19381 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19441 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19501 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19561 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19621 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19681 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19741 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19801 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19861 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19921 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    19981 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20041 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20101 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20161 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20221 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20281 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20341 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20401 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20461 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20521 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20581 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20641 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20701 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20761 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20821 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20881 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    20941 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21001 ataaatatct aatgttactt ttttaatttt tttttcgaat acgcgccatt acaccttcta
    21061 gttctaactc tttgtcttca gtgatgtacc aaccgcttgc ctgtgtccct ccggctgcct
    21121 gcggtaaata atccaatttt gttcgaaccc agtgcttttc ttctgtaaaa tagtcttcta
    21181 ccaagacctc tagatccatc cctgctgaaa gaggcaaatc ttcccgaaca gatttaaaga
    21241 taaatcgttc cttcttccca tcatacttca atattcctgt atcgataatc ggtaaagaat
    21301 aataatccag ctctttcttc accgtcaata aatgtttgaa aacttcatgg tacacttgct
    21361 ctaattggcg accttctgaa ttgttcgggt aattaaccat cactttttct ttgtctggat
    21421 catagaaggt ttggcttagt tgagtgatat aagtatgtaa tctttccatt tctgttttta
    21481 acttttctct ttgaccttct tctaccttca tatactcttt caccctgttt tctttttaga
    21541 tattttcttt cttattttac tatagttatg tgcgttaagc gatgaaaaac agcaaagatt
    21601 ggaaggatct ttgctgtttt ttcttaaaat atccagttga gtacaggatc aatctgtttc
    21661 attgtcctct tgatccgtcg taatattgat cgatgtgtct ttagcagtga tctttagctt
    21721 tttctgtcct tgttctaaca aagtatttgt tattggctgg tagtcttttt caatataaga
    21781 ttcaccagac gtttggatag tatagccact gttttccatc tcatgcaaag ataaattgcc
    21841 accgtttagg atgacttttc cttctttgct gatcaaactg tcttctagat ggaagttgct
    21901 gttttcacct gtgacgatca agttatccac attggcctca tctaatcgaa gtgagcctaa
    21961 ttcatctttc gcagttattg ttcctcctgt aaattcattg aacgataccc agccatcatg
    22021 tgtcttagta ttcacttttt tgattgatac ttcgttgata tcaattgcac tattattccc
    22081 ttcgattgtc aattcagata attcatgatc tgcagggaca acaatagtga tttgtgcact
    22141 tgtgtcttct aaactaatga ttgcacctgt tgattttttg actgcattca cagtcaacac
    22201 gccatttttc tcctttattt ctggcttttc tgtcttatca taagtcgttt ttatctcaaa
    22261 atccaatccg cgtatgaccg aaactgtttg attttctgca tggatgacga ttttgtttgc
    22321 atccttgatt ttttttgtct cagtaaccat atcgatcaac cgaggaccat tatcccaagt
    22381 cagactcttt tgagcgccta atgtataagt tgcacctgct aggaggccac cagtaagcat
    22441 cagaccaata ccggtaatca aaaaatgttt caatctcaat ttatgtgtcc tccttcaaac
    22501 gcccgctgaa tgcagatctc tttttgtaat aaaacgtcgt cccatccatt taacaaagtt
    22561 catcatgaca taaaaaagcc cttttgttcc tttcagcaac agtggcgaaa taaatactgc
    22621 gcttcctgtg gcgaacagac cgacaccgat aaagaataat ccactcagaa tcgattgtcc
    22681 aaacacactg attcctgaga tgatcatgaa taggccaaca cctaagatac tgatacataa
    22741 aacatagata ctaaaaatga gcgctagtaa agcaaccaat agtcccccaa taaccaatat
    22801 aaaccccagt gctagtggaa tgaggacagg agaagcaaat aaaccgataa tgattaacca
    22861 aatcaggcga atttgtcttt ttggattttt cactaactcg ttttctgctt gttcttcttc
    22921 tgaaaagtaa ctgattttta attttaaggc aaactgcttt ggtgtaccat attcatcaat
    22981 gatctgatct aatgtttttc cagaatctaa aaaatattcc tcataatact ggatcaaatc
    23041 aaaacgatcg tcttcaggaa tttcttgaag atgatccaca acttcattca aatattcatt
    23101 catttgtgct ctcctccaat aaccggtcta aagaatctcg aaaaacaatc cattcatcaa
    23161 taatttcttc taaatgacga cttcccattg gtgtcaacga gtaatatcga cggtttctgc
    23221 cttcaaaagg ttcatcatag gtctcaagat atccttcttt tttcaatctt ctcaaaaccg
    23281 gatatagtgt agattcactg acagagagac tttcttgtac gatttttgtt aaggcatacc
    23341 catacatatc ttccttgctt aatgctgcta gcacagtacc atcaagaaga acagttggaa
    23401 tctggatgtg cattacgtca ccttctattc tcaagtgtaa tattttattt caatctatat
    23461 tatacaacat ataatatagt ttcaaaatca gtttgtcaaa tacaaaaaaa ctgactaaaa
    23521 gatttgatcc tttttagtca gcaaaaaatg gcttctcttt gatttttatt ataacagtgg
    23581 cataatccaa tttgtccaca tccagctcag tggcatgata acgatcattg caggtatcat
    23641 gtcagctact gggaacattt tcacgttgat cattctaaaa ccggtagcga ccatcaagaa
    23701 tccgccgcaa gctttaaaat ctaagatcat gtctggtgta gtcaatggat aaataaattt
    23761 tgccaataag aacaaaatat aaaaaataat gaattgggga attgctatca cagaaacgac
    23821 ataccctaaa ttacacgcaa aaatagctgc tgtgaaaaaa tcaaggacag atttagaaat
    23881 caaaatcgta ctgtctcctg tcatcccaga atctaaactt ccatagatcc ccgtaccact
    23941 tgcacaaaat agaacaatga ccgtcactaa tgtgctgata aattcatcgt gggtcatacc
    24001 aagcttttca ctagggaaga ttttagaaat aggtacttgc atcagcgcag cccccttatt
    24061 tatcccatta cctaaatgaa caatcaaacc aatcgctgtt cctatcacta aagcaaaaat
    24121 aacagccggc atatttttca tcggcgcaat ggaatagatt cccatcccca tcgaacacac
    24181 accaaaaacc atcgtcagct gtgctttaaa atgttcactt aatttatttc ctactaatcc
    24241 gccaattagt ccgccaaaca aaacggaacc tacattgatc aaaactccag ttggcatcaa
    24301 aaaactcctc tctttttcta cgaagcgaaa aaaatcagaa aaatttctgt aattattgct
    24361 ttcgctgcaa gaaagattgt aacagacaga gaagattaca ccaattcatt ttttgtgtta
    24421 aagtattctt tattggaata agtaggagga atatgatgga tatccataaa ttcgaagttt
    24481 ttcttgattt agccgaaaca atgaatttca ccaaaactgc tgatcgacaa ttcaccaccc
    24541 aaggaaatat ttctaaacag attctttcgt tagaaacaga attagacgtc aaattattcg
    24601 aacgcgccca tagaaaaatc gaattgaccg aagctggaag cttattgctc ccatatgtaa
    24661 aaaatgtcgt agaacaatac catgctatgc aagaagtgct tgaaacctat acgaaagaca
    24721 acaatctttc attaaatatc ttaacgatac cgacaatgat caattataaa ggtttttcca
    24781 aaatcaccga gtttttgaaa cgccatcctg aatttacagt ccagttgaaa gaagtggaaa
    24841 gtgtcgaatt aacttacaat gacgccaatg aacatagtga cacgatccac tttgctagaa
    24901 gtttccaggc cccaccaagt tcgattgagt ggttgcctac tgaaacagac gattttgtag
    24961 cagttctacc taaaaatcat cctttagcta gtaaaaagaa attggatcta agcgaactga
    25021 aaaaggagaa ctttttgctc cttggcccca ctacgaactt gtatcaacct gtactagatc
    25081 tgtgtcgcga agctggattt gaaccaaaaa tctcttataa aggagcaaga atcgacttga
    25141 tcatcaatat gattgcaaat gacatgggga ttgctattgt tatggaaaaa acagtcaaga
    25201 atctattaaa agaaaatact gtgattctcc ctatttctcc aacaacagag agttatcttg
    25261 catttactcg taaagtcggt gagcattctt tggcttcaga tacattttgg gagtatttaa
    25321 atcaagaaga aaactaagcc ctacattttc tgtccatcta tccacccaaa aaaagagagc
    25381 gacgcatgac tttcgctctc tttcttgcat catgtccttg tttcaatgac acctctaaca
    25441 tttctcgtag ttcaataaga aagcagcgat ttttatgcca aaagaaccgt aagaacattt
    25501 ccctaatcac aaaaaaacga ctaaaaaccc ctaaaaacct tcaatagaat ttttaggggt
    25561 tttattcttt ttcctattgt ttacttaacc gtatcattgc tgtcgttcct actccttcga
    25621 tactgctata atcaatacgt ccatcatgca gttctagaat ttttttaacc agtgaaagtc
    25681 ctagcccatt cccagaaatt tgacgacttg tatctccttg ataaaatttt tcaaacattc
    25741 gatctctggt ctcttcattc attcctacac ctgaatcggt cacttccagt acaatctctg
    25801 tggctgtctc aaatagtttg atatgaatct gtccattgat ttgattgtac tttatggcgt
    25861 tatccatgat attcagccac acttggtaca aaaactcttc attgcctgtg taatttactg
    25921 cagcaagttc gatatccaat tctagctgtt ctttctccca tttaggctga aaaaaaacga
    25981 ttacttctct gatttgttca tctaatcgat actctttctt ttccagttgg atacgctggt
    26041 tttccagttt gttcaatttt aagatgttct ctgttaattg tgaaagctgc gtgatacttt
    26101 cgataatccg atataaaaat atttggcgtt cttcgtcaga gagatttggg gcttgtaaca
    26161 attgaacata tccttggatt gttgctagtg gtgttttgaa ttcatgggat acactggaca
    26221 caaagtcatt cctcagtgtc tcgatactat tgagttcttg gaccataacg ttgaaatctt
    26281 tatacagttg ctgtacttct gctactttct gctgttcatc cattcgaatc gaaaaatctc
    26341 ccgttgctac cagactcata tttgtcccta gcgcactgat tggatgcagg atacgccgcc
    26401 caacaaaaat cgatattcct gtacccacga ccatactgaa ccccgcaaag acgaataacg
    26461 ggtataacgt tggttttcca gccgattcag caggcgagat agaatgtcga gacaataaga
    26521 aaataaaacc gagaaaacac agcagtgtca ctaagatcga aatgaagaca atactagcaa
    26581 aatagatcca taattgtgaa aatagctttt ttttcatctt taaataaccg ccttatagcc
    26641 tagtccccgt actgtgatga tttgaaaatc ttccgtcttt tcaaaacggg tccttaaacg
    26701 tttgatatgt acatcgatcg ttcgttcttc tgtattcgta tctaatcccc aaatatcatc
    26761 catcaactgt tggcgagtga agatcttgtt gggataagac agtaatttat acagaagcag
    26821 aaattctttt tgaggcagaa tcaacgaatg ctgatcttgt aacactgtat aagcatctag
    26881 ctctaatttt gtttgtccga ttatcaagct actgctgtaa ttgatctttg aacgtcgaag
    26941 caatgcttcc acacgtagga tcatctcatt gacatcgatg ggttttgtca tataatcatc
    27001 tgctcctaat ttgaacccgc gtttcttatc ctcaaaatcg gcctttgccg taatcattag
    27061 aacaggtgtg tcaaatctgg tgctacgtaa tgagtctagc aagtcatagc catccatttt
    27121 cggcatcatg atatctgtaa tgattaattc tatacgtgtc gcttctagtt ttttcagcgc
    27181 atcctctcca ttcaatgcgg tgatgactga aaagcccgct cgttccaaca cagaacggta
    27241 aagcaactgt aattgcggat catcttctac tactaaaata cgattcattg atctccctct
    27301 gttttttgaa aaatttctag attttttctt gtagttcatc ttcagttaac atttatttcg
    27361 tatactacga tcattgttct gatatactta ttagtatacc gaatatttca atacgggaga
    27421 agggtgatta tgcttcaatt aaaagatata aaaaaatatt ataaagtcgg cgaaaccacc
    27481 accaaagcat tggatggtgt gtctgtcgct ttcagacaaa aagaattcgt agcgatttta
    27541 ggtcctagtg gttctggtaa aacgactatg ctgaatgtga ttggtggact agataattat
    27601 gattccggtg atatggtaat caacggaaag tctactaaag actttaagga cagtgactgg
    27661 gatgcctacc gaaacaactc tatcggtttc atttttcaaa gttacaactt gattggtcat
    27721 ctaggcatta ttgaaaacgt tgaacttggg atgacattaa gtggtgtttc taaagacgaa
    27781 aaacgaaaac gggcagaaga tgcattgcac cgagtcggct tgacaaatca tatgcataaa
    27841 aagccaaatc agttatctgg tggacaaatg cagcgagtgg ccatcgctcg agcactagcc
    27901 aatgacccag atatcctttt gtgcgatgaa ccaaccggag cgttagatac agaaacaagt
    27961 atccagatca tggaattgat ccaagagtta tcgaaagaaa aattagtgat catggtcaca
    28021 cataatcctg aactggccca tcaatacgca gatcgaatca ttgaattttc tgatggtaag
    28081 atcttaacag attcccatcc tcacatcgaa cgaccaaaag atgatcaatt taacttacgc
    28141 agaacaaaga tgagtttttg gacagcgtta aagctttcct tcaacaatat ccgaacaaaa
    28201 aaaggacgta ctttcttgac ctcctttgct tccagtattg ggatcatcgg aatcgcgatc
    28261 gttctgtccc tttcttctgg attccaaaaa caaatcgata acacgcaagc tgaaacgatg
    28321 gcgaaatttc cgattactat ttctaaggta acgactaacc agactcgtga cgatgccgga
    28381 ttgggtgcaa gcaaagcgga ttatccagac agcaaaacga ttactgcaaa agtcagcgaa
    28441 gaagataaag cacagcacac gaataagatt gatcaaacat acgtcgatta cgtaacagac
    28501 atcgatccta acttaagtaa taatatcgga tttacccgta ctacaggaat caatctttta
    28561 cgtgatgtaa atggcaaagt acagcctgtc agcttctcga atcaaaatcc tgatacggaa
    28621 tctctttctt tatctagtac gatgtcagca atgacaggtg tgggagtgtc aagtttccct
    28681 acacagcttg acactagcaa agaaaacttt ttaaaagaca attattcttt acttgccgga
    28741 agttatccag cctcagcaac agatgtcgtg ctgatcgtag acggcaataa taatactaat
    28801 atcaacgcat tgaagaatct tggatttgat gtgaaagaag acgaaaaact tgattttgat
    28861 gatatcgttg gtacaacatt caaattagta aacaacaata cttactatac aaaacttcca
    28921 actgggaatt ttatccctaa tacagattat gatgcgatgt atcagaatgc ttctgatgaa
    28981 ttgaagatct cggggatcct acgcgtgaaa tcttcttcta caatgaatct cttatctcca
    29041 ggaatcgctt atagcgatca attaacgact caaatcgtca acgaaaacaa agaatcagaa
    29101 atcgtcaaag ctcaaagaga cagtggtgta aatgtcttaa ctactgaaaa agttgacgaa
    29161 aatgccaaac aaacattgct tagttattta ggtggagaca gcttgccttc aagtatcatg
    29221 atttatccga acaattttga agacaaagaa aagatcttgg attatctaga tgattacaat
    29281 aaaggcaaat cagatgaaga taaaatcatt tatactgatt tagctggaac gatgactgaa
    29341 ctgactggcg gattgatgga tgctatcact tatgtcctta ttgcttttgc agggatctct
    29401 ttagtaacaa gtatgatcat gatcagtatc attacttata catctgtgat tgaacggaca
    29461 aaagaaatcg gtgtactaaa agctttaggt gcacgtaaaa aagacatcac tcgcgtattt
    29521 gacgcagaaa cgtgtatttt aggtatttct tctgggatat taggtgtgtt tattgcatgg
    29581 ctagctacgt tccctatcaa tagcatctta tacaacatga cggatctaaa aaatgttgct
    29641 caactaaatc ctgtccatgc gattattcta gtgatcgtct ctactatatt gacgatgctt
    29701 ggcggtcatc ttcctgcacg aatggcagcg aaaaaagacg ccgctatcgc tttgcgtgca
    29761 gaataaggtt gtgacaacca catttagatt tgagtcataa gagcgctgaa atagtagaaa
    29821 acagcaagaa acgaggaagt aatatgttcc ttgtttcgcg ctgttttttt taattatttt
    29881 ttataattct ggtatacttt tctttccttt gtaccacaac tcttctggcg tatacctgtc
    29941 ttttccattt atgaatactg ttccatctgc ttcaatcgtt acttcagtcc caatccacat
    30001 attacgagaa agtgcgagaa gattttttct ttttcctctt cctttttttc caaattgcaa
    30061 ggcatcggtt atctcttctg tccaatgaga tttcacgtat tttacttctc cgttttcaaa
    30121 tgtaactttc aaaatattcg ctatattttc cacttttact gctttaggaa atgactcctc
    30181 catttttttc actttccttc ctccaacact aagtatgcgc tttcttttag tgataagtat
    30241 atcatagttt attctattat caaaaaaacg catccattcc attgtagaat actttaccga
    30301 acaagttgaa ttgttttcct ctttttcacc ttttactata gataggtaaa gacaactaag
    30361 gaggaatctc tcatgacaga tcaaccgtat gatttaagaa aagtcatcga agaactaaaa
    30421 acacttccag gtcaatatca cgaaacagat attgaaatcg atcctgaagc agaattatct
    30481 ggtgtttacc gttatatcgg tgctggcggt acagtaaaac gtccgactca agaaggacca
    30541 gctatgactt tcaacaatat caaaggtttc cccggcacac gtgtgaacat cggaacaatg
    30601 gctagtcgta aacgtgtagg tgaaattttg caccatgatt atcgaacact tggtcgtttc
    30661 ttgaaagacg ctgtagaaaa tccaatcaaa cctgtgaaag tagacaaagc agacgctcca
    30721 gcacaagaag tcgttcatct agctaccgat gcagacttcg atattcgcaa cattttgcct
    30781 gcacctacaa atactcctga agatgctggt ccatacatca caatgggtgt agtcttagga
    30841 tcagatccag aaaaaacaat gagcgatgta acgatccatc gtatggttct agaagataaa
    30901 gatacgatcg gtatgtacat catgcctggc ggacgccaca ttggtcactt ccaaaaacaa
    30961 ttcgaagcct tgaataaacc aatgcctatc actatcaata ttggtttaga tccagcaatt
    31021 gccattggca cgactttcga accacctacc actccacttg gctacaatga actatggtgt
    31081 gcaggagctt tgcgtaacga acctattcaa atcgttgatg gtatagcagt cgatgaagtg
    31141 ggtattgctc gtgctgaatt catcattgaa gcagaaatca tgccaaatga aacaatgcaa
    31201 gaagatatca acacgaactc aggaaaagca atgccagaat ttcctggcta tactggggat
    31261 gctaatccag ctgtacaagt agtgaaagtc aaagcagtta ctcaccgaaa agacaatcca
    31321 atcatgcaaa caacgattgg tcccagtgaa gaacacgtat ctatggccgg tattcctaca
    31381 gaagcaagta ttcttgcctt ggtcgataaa gctattccag gaaaagtagt aaatgtctac
    31441 aatcctcctg caggtggtgg taaattgatg accatcatgc aaatccgtaa agaaaacgaa
    31501 gcggatgaag gtattcagcg tcaagcagct attcttgctt tatcagcctt caaagaattg
    31561 aagacagtca ttttagtcga tgaagatgtt gatattttcg acatgaacga tgtagtttgg
    31621 actttcaaca ctcgtttcca aggagataaa gatatcatcg tgatcccagg aatgaggaac
    31681 catccattgg acccatcaga attaccagaa tataatcctg gctctattcg tttccgtggg
    31741 ataagtgcaa aaactatctt tgacggtaca gttcctttcg atatgaaaga acattttgta
    31801 cgtgcaaaat tccaagaagt agaagattgg gaaaaatatt tgaaataatg aatgatctgc
    31861 aactgttttg acatcataaa aaggagtgcg gaaaatggaa caattggcac aacggaagaa
    31921 aaaaatcgtg attggtgtaa gtggcgcttc aggcaccatt tatgccatca acctagtgaa
    31981 gaaactaaaa gaatatccga tgatagaaac acacggaatc atcagtccat gggcaaaaca
    32041 gaacttaaag ctggaatccg acttatcact aactgaattc gaaagcctgt ttgattacca
    32101 ttattcaaac aaagatttag gtgctgctgt cgccagcgga tcatttttga ccgatggaat
    32161 gatcatcgta ccggcaagta tgaaaacagt cgcaggtatc tctgttggtt taggcgacaa
    32221 cctgattgca cgtgctgctg atgtaacaat gaaggagcaa cgaaagttag tcattgttcc
    32281 tcgtgaaacg ccattaaatg ccattcactt ggaaaacttg actaaattag cacgactagg
    32341 tgtgcaaatc ataccaccaa ttcctgcttt ttataatcag cctcaaacga tccaagatct
    32401 tgttaatcac catacaatga aacttttgga tcaatttgaa attgtaaatg agcattcaaa
    32461 acgttggcag ggtgattaat atggaaaaag aaacgatagt tgaagtaacc aaactaggtt
    32521 tcacgatcca agctgctgtt aaagtaatcg gtaaagactt attgatcact ttaacaggcg
    32581 gagatacacc acatatcgga acagtcacta cattttctcg tgatacagag atgcaaactg
    32641 tacgtttccc tagccacgat ggtcggttcc ataaagacga tgtcttagcc gaaaaaatag
    32701 ctgtgatcat caaaactgcc gttccgggaa gttgtacgat cacttccggt atccatgttg
    32761 accatatctc caatgcacaa atcaacgcct cttttccgat gtcagaagag ttaggttaca
    32821 agattttaga ttggttggaa aaaatagatt tccatatcaa ataaccagtt tattataaag
    32881 atggtgaaaa accattatga aagaagaggt tgtgaaatca gcgttatgac ctgagtatga
    32941 gagaaaaaaa tggaaaaata gcttctcata ttttgccgtt tttgagctaa taccgaggga
    33001 cagctgattg aacgccgttt atcaagaggt tgtgataaag ccgcttcgtt tcgagtagta
    33061 agaaaaattt tgtaaacatg ggtttccaca tttttgcata aatttgactt actaccgaga
    33121 aactggcttt tgaacaccgt ttatcaagag gttgtgacac gtcttttgtc acaacctctt
    33181 ttatttttgc ttcactgtaa ctgctacaat atcaccgatt tcttttccga tgtttttacg
    33241 aatatctttt cgtagtccaa ggatgtagca gactgaacca tctggatttt tcacgcccat
    33301 attcacgata ctgccttcat acggcacatt gtcaaaagtt gcttggactt taactcttcc
    33361 agcaccgaat tcttttctta tatcatacgg aaaaataata tatgccccgc ccttgccgac
    33421 ttcggaagca tagatttttg attcaaaaga ataaatttcc ctcattttat ttctcctatt
    33481 ttttacatct agtatagcat agcaaaaaaa agaaggaact gtgacaaaag tcttgtcgca
    33541 gttccttttt tcgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatag
    33601 tcaacaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttaaacggca
    33661 ggctcacaac ctcttcctta aacggtgttc aaaagcttgc atctacggca ctaagcccaa
    33721 tcagatgaaa aatatggagt gctattttta tctgattgtt cttactgctt gatgcagagc
    33781 agctttttca caacctccta catggtgttc aaaatattat tcgccttttg cttctttcac
    33841 taattttgcg aatgcttgtt gttggaattc agcagctaag gctttcgtac gttgttcttg
    33901 ctctgctttg ctcatttttg gattttcagt cattgctttt accataccgt cttgaacata
    33961 agcttttgct tcactttcca tcgtactttg ctgtttcttc actaattcag ctaacttggc
    34021 tgcttggtcc gtacttaatt ctacccatgt ctctgggatt ttgaatgtat tcactcggct
    34081 gtcatattcg tgattgttat cagcaatatt gaaccagaat ttatacatgt cgtttttgta
    34141 ttcccaataa gtagttttcg tcactttttc agcatcgccg tctgtctttt ctactttatt
    34201 tgtttcgtta tccgtaccag ttgcttttgg tttttcttgt ttttcatttg ttcggtaaag
    34261 atacactttt tcttttccat tccctaaagc tttatacaaa agcatatctg ctccttggga
    34321 atcagcactg gaaacaattt tttctgtttt tgtttccgtt actttttcca ttccaaagtg
    34381 attggacaaa ttagcgacga tcaaacttag tgaagcgacg aagattagtc cgaaaatcaa
    34441 agataaaaag gtttgccatg tttttttagc gaacacgttc gttaaagcaa aagcaagcaa
    34501 gctgacaatc aaaactccaa taatcatgca ttatcccccg ctttcattag aacaggttct
    34561 tttttcttca ataaaaaggc aataataaag ccaagaatcc caaagatgat cgctacgata
    34621 aacgcggcat ggtaacctga cagtgtggca ttgattgcct gatctttata agctaatggc
    34681 gtattatcca atacagattt gcttgggata tttgcttttg tcacattcgt caacacactg
    34741 atcaaaatag ctgtaccaat cgagcttgcg atttgacgga atgtattatt taccgctgtc
    34801 ccatgactga tcaagttatt tggcaatgca ttcataccag aagtcgttac cggcatcatg
    34861 accatggaga tcccaaacat acggatcgca taaaggatca cgatatataa aactggagtc
    34921 gtttttgtca agaaagcaaa tggtgcagtt gccgctgtca aaatgaacat ccctgtgatg
    34981 gctaaacgtt tcgcgccgta tttatcgaaa atcgcacccg tcacaggcat catagccccc
    35041 atgatcaatg cacccggcaa caacatcaag ccagaatgga atgctgattc tccacgaata
    35101 ttttgaatat acaatggtac gaccatttct gcccctacca tcgccatatt tgttacacca
    35161 gctaaaacag ctgcaatcgt gaatgtagat gatttgaata cacgaagttc caagaatgga
    35221 tgttccaaat gtaattgacg ccatgagaac aaggcgatga aaatcacacc aacaactaag
    35281 aaaccaatta ctttcaagct tccccagccg tcatctccta cactagaaaa tccgtaaagt
    35341 aaactaccga atccaatcgt tgataaaata gcggataaaa tatcaatgct aggatttgac
    35401 aatggaataa cacttttcat taggaagaat gctaaaacca agacaagaac aacgattggc
    35461 agtaccatac caaacaagtc acgccaatgg tagttatcaa tgatataacc agataatgta
    35521 gggcctaaag caggagcaag tccaataacg atccctgaca tccccattgc tgatcctctt
    35581 ttttctggcg ggaaaataga tagcatgata ttttgcaata atggcatcga tactcccaca
    35641 ccgcaagctt ggatcaaacg accgattagt aatgtactaa aattaggtgc tgtaaagcaa
    35701 gtaatcgttc cgatcaaaaa gatcgtcatg gcagaaagat ataaatttct tgaactgaat
    35761 ttattgatca gccatgctgt tattgggatc atgatcccgt tgactagcaa gaaaccagtc
    35821 gttaaccatt gcacatcaga cgctgaaata tcgaaagctt tcatcaatgt aggaaaagct
    35881 gtcgtcaata aggtctgatt caaaacggta caaaatgtac caattaataa gacaacaact
    35941 aaaagtgtcc gattatatgg ttttccgtaa atatctacgg gttggttgtt actcatattt
    36001 ctccttcttt ctgaattttt tctgaaacta ctagaatact ttagtcctaa attcgctatt
    36061 tgtcaaccta cttgacacat aagttaatac ggatataatt aagggaagca agaggacaaa
    36121 aggagtgaca aaaatggttg gggcttccct taaacgtttg ttggaaaatg atcaattggt
    36181 tgttggcatc agtgaattaa gtgatatcgt aggtgtgtct ccacgtcaat taaggtattg
    36241 ggaacaaaag ggattcattc gttcgatcga agctgatgcg aattgtccta gaaaatatcg
    36301 tttacctaca gtcataaaag tagaactgat caaaaaatat ttagatgacg gatttacttt
    36361 gtcaaaagct gcagaaaagg cagaaatccg ccaaaagaaa atgcaccatg tccgtaaagt
    36421 tttttcaaaa gcaatcaaag atatcgaatt gatcgacgaa cgctatacga ttttacaaat
    36481 cggtaacttc aaagaagaga atgctaagat gtacatcatt catgacaacg agacagacga
    36541 attaacttac aagctccttc ctgtcgatga aaaagctgac tacgaacagt tgttaaaaga
    36601 actctaaacg ccactaatct cctaagtacc aagcttttta ataagtagat gctacataaa
    36661 tacttgaaaa aaggcctgtt tgaacataag aatatccgaa cggtgatctg cgtttctttc
    36721 atatagaaag gaattctcca tcactgttcg gatatttttt tatttttttc ggttactttc
    36781 ttctaataca tctgttagcc aatttgcata ttctgtttcc cgattgatcg catgcgctaa
    36841 aatcaggtag tgtccgtaat tttcatgttt ttctttttca gtaggaaaga tcgttttttt
    36901 tcgctctaat agatgaagtc gtttttcttc atgaaggtca cgctgctgct gaataatttc
    36961 tactaatgcc ggatcattca cgtctttgac gaaatacaat ttcagaacaa attcgtctcg
    37021 attaaccggt agttcattgg tgggtgtatg aatccactct gccaattgtt ccttcccttc
    37081 ttctgttaat tcataaactt tcttttccaa tttttcacct gtgatctcta cttgatgctg
    37141 aatgattcct tgctcttcca tttttgcaag ttcaggataa atctgactat gttttgcttg
    37201 ccaaaattct cctatttcat ttttgaacga ttgattcaat tcatatcctg tcatcttctt
    37261 cttacttaat aaccctaaca aaatatatcg aagtgtattt tttcttgcca tcagttttcc
    37321 ctccttgaca aatctttaaa tccattctat cataaatgag taatcgaaaa catgtagttt
    37381 attacatgtt ttaatttata aggagatgct taaacatgaa agaatttaaa gaattggaag
    37441 attttattgg aactcacttt atctatacgt atgacaatgg ttgggaatat gaatggtatg
    37501 ccaaaaatga ccatactgtt gattaccgta tccatggcgg aatggtcaaa ggccgttggg
    37561 taaaagacca agaagctgat atcgttaaat tgacggacgg cgtattcaaa atcacttgga
    37621 cagagccaac agggacagac gtggcattgg actttatgcc aaatgaaaag aaacttcacg
    37681 gaatgatctt tttccctaaa tgggttgaag aacatccaga aatcactgtt actttccaaa
    37741 acgaacatat cgcagaaatg gaagcagctc gtgaaaaata tgaaacttat ccaaaattac
    37801 ttgttcctga atttgctacg atcacttata tgggtgatgc aggtatagac aatgaagatg
    37861 ttattagcga agaaccatac gatggaatgc cagatgatat ccgtagcgga aaatacttcg
    37921 atgaaaatta taaacgagta aaataaggtt gtgaaagaga ggttgtgaaa tcagcgttct
    37981 gacctgagta ttagagaaag aaacggaaaa atagttcctc atgttttgcc gttttttgag
    38041 ctaataccga gggtcagctg attgaacacc gtttatcaag aggttgtgaa atcagcgtta
    38101 tgctttgagt aataagaacg ctagttagaa aatagctttt catattttct aggctagtgg
    38161 gcttattacc gagaagcagc cgtttgaaca ccgtttatca agaggttgtg aaatcagcgt
    38221 tttgacctga gtattagaga aacaaatgga aaaatagttc ctcatatttt gccgtttttt
    38281 gagctaatac cgagggtcag ctgattgaac accgtttatc aaagattgta acaactgcat
    38341 attcacacag ttaaattagt gacttcaaaa tgaaataatg ctcccagcaa aaaattaggc
    38401 tgggagcatt atttcgttat tctgttcatc aaaaatcagc gttttgattc catttcatca
    38461 caagttcctc ctcatttact gcttcatctt tttgctgtgt ttgaattgta aaaccttgtg
    38521 aaagatagaa atgaatagct gattgatttt tttgatagac tgtcaaagtc agtgttttat
    38581 tggtttcttt tatttttttc attagttgcg taccgattct ttgttgccgg tattcccttt
    38641 tcacaaagat tcctgcgata aactgatcaa tcaccccagc aaaaccaata atttcttgat
    38701 ctatttctgc taagtaaatc actgcatctg gtaaagcttt cctaacagat tctacttgat
    38761 tctcccaata agttgatgga atgaattcat gagaatccaa gttagcatgc agccaaatcg
    38821 atagaacaga ttccagttct ttggcagaca attttgttgt tgtgcggatc attttttctc
    38881 tccttactta ttcattgatt ttaaaataaa aaccattttt ttgaagctct tttacaagtt
    38941 ttcgcccctc aaaaaagtat atcttctctt gttttacttt tctaatacca aaagaaacag
    39001 caactttagg atcatcagct gaaacgattc gataagttcc accaggcaat tgatccagct
    39061 tgactttccc atgatggtcg ctttgtactg aaaacggcac accatttcta gtaagtgttt
    39121 ttttcttatg tttcaaaatg aatgaactac catttaccgg tttccctgac gaatcactaa
    39181 taataaatga aaaatcaagc acttttttac ttaatctata ctgatagatt agtcgtccga
    39241 taaacactaa cagtatgact acagctatta tcgttcctgc aagaataaac cattgccgca
    39301 tggttctttc ctgatttcct tttccttctt ctttttttac cgattcgtta taaggaatcc
    39361 gatgtcctgt cactagcaaa cgatgcgtgt tgatcatata cggcgtacat gtaagcaagg
    39421 taactaagtc ttctccttct gaaatcgtta gaacagacgt gtcatctggc agtaccactt
    39481 gtatattttt aacttgataa gccaatttct cccccaaaac agtcaaaata aacgtgtctc
    39541 cgttttctaa acgatctaat tcggtaaata accgtcgatt aggtaaaccg ctgtgagcag
    39601 agatcactga atgcgtcgaa tttccaccaa gcgggaaaga ggttccttgc aagacgcctg
    39661 ctccattttc aaggatccta ccattgagcc gatcataaag tgaaatattt aattggatct
    39721 taggtatatt gatacttccg atcagccatt cctctaattg gctggatgtt gtactttcat
    39781 ttctgttctt ttcattgaaa ggatcagtac caggattgaa tccttggtca gttaatcgtt
    39841 ggtttttctc ctccattttc tttcgctgca cttcaatttc agctgcggtc cgtttttgta
    39901 cttgttcgat acgtttttga tcaataaaac tatttaaaga atccacataa aatggatata
    39961 aagcgatcag taaccctacg acgatacata ttaataaaaa caaacgaaac aaaagtcgac
    40021 gagactgttt ttgcttactc tgcttcacat gtgtcctccc ttgcaagttt ttttgttctt
    40081 tcttttatta tatgggttca ggaaaaaaat agaaaatgaa agccgatgag gttgtgaaaa
    40141 agctgtcctg catcaagtaa taagtacagc ccaaaaaaat agctactcat attttttgtt
    40201 gggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa gaggttgtga
    40261 aaactgcgtt atgctttgag taataagagc gctagtcaga aaatagcttc tcatattttc
    40321 taggctagtg agcttattac cgagaagcag ctgattgaac accgtttacc gagaggtcat
    40381 gaaaaagcta ctttgtttcg agtattaaga aaaattctgt ttcaaaacaa ggagatgcaa
    40441 caaaacttat gtcgcatcct ctctccttag aactttcact tatgtttttc ttgtaaaatt
    40501 cataaatatt ttcatgaatt ttctaatttc ttgcctacct ctcgcatagg atatatgatg
    40561 aatttatcta aagtttgcaa aggagttcga caattgaaaa aatatggccc atttttagca
    40621 atttttttca ttgctttggc atttctcatg cctcatttat tcacacataa actaattttg
    40681 ggtgctgatg ccccattcca ttacaatcgg ttctatgatg ccgcgcaaca aattaaatat
    40741 ggggatttcc attattttcc ttccctttat ggattccagc aaagtggcag gatcgtgaat
    40801 gccgtttacg gcccattttt cgcttattta caaggattga tcgtgttgct tgcaggtagc
    40861 tggtttcatt atcagattct ttcaaatttc attctttatc ttatttcagg attttctatg
    40921 ctgattttac tgagatatgt caatgttcga aattgggtaa gtgtaccagt agccgtttta
    40981 ttcatgagta cgtattctat tcaatactgg tctatcaacc aaggattcac tagctggggg
    41041 acttgttttt atcctttatg tatgatccct attgctgatt tcgtccttca taaaaagttc
    41101 cctgttatca aagtcgctgt ctcagtggga cttatgacac agatccattt actgtcttcg
    41161 atcatgttag ctttgatgta tatccctttt tatctttatt acttttttaa ccagactgaa
    41221 aaaggaaaag cactgcttca actgattaag tcggtcttgc tttattttgt tctgacagca
    41281 aatatttggg caggattagc tattgtttac agtgggaata agattcttcc tccctttgtt
    41341 aacagagaaa tgagtcaaaa ggcgatagat ctggatggca gttattggct ccattaccct
    41401 aaggcctttc ctatattgat attatcaggt tttttgcttt ttattttctt tcaacaaaag
    41461 aacaccttat tccaaaaaat cgtgttgtac acaagtcttg ttttcttttt tctttctact
    41521 agcttgattc catggacgac attgatcgaa aaaaatgtgc cttttattag tttgatccag
    41581 tttccatttc gatttttcgt gccatttacg ctattgcttc tactttacct tgccctttca
    41641 ctgaaccagt ggtctgaaca aaagtggttc cgtcttttaa gtatatttgg cattcttatt
    41701 ttttcagcac agacgatcca gaacttatac cagcatctgg aaaagtggga aaatgaaaca
    41761 tttgtcagtc gacatactta tctattcgat acgccggaag aagcaaggaa aacattcttc
    41821 agcaaagata tgtctaccag cttgttcgtt ttccaaaaaa ccacacctga ctatctgcca
    41881 atctataagg agacaaaaga caataaatac gaccggtata acgaagaaat actagaaaac
    41941 gaaacaactt ttataaaaac acatccaggc ggtcttttga caatcaagtg gaaaaacact
    42001 ggggacaaaa ctgttcatat ccctgttatc gtctacgatc ggactgtgct acagcaaaat
    42061 ggaaaaacat tgactgatta tgaagtaaca gatatcggga ccccaatcgt gaagcagcaa
    42121 aaaggaatca acgagctcac gcttcattat caaacgccaa tctatttcta tttcattctt
    42181 tctcttactt tgattggctg gtttaccttg ctatgtttat ttatttatca tagatacaag
    42241 cttttacgtg cttaggcaaa agggataaat acatccgatc aaaaaactct ccaaacggta
    42301 taatcgtttg gagagtttta ttttaatcag tttttttatg ttatatgtta tattttttat
    42361 tcgttcacat ctaattcgat ttccaccccg tagtgatcac tgatgattgg actatcgatc
    42421 ccatcaaaaa tccgttgata ttttttaacc tgcattcctt cagggacgaa aataaaatct
    42481 atacgcaatt tttctgtatt ttgttcccaa ccatctattt tcttttccac agtagctgct
    42541 tttccttttt cattcgcgac aacatacgaa tcttgaagaa gaaggcaact ttctcttaca
    42601 gtataataac cttgagagtc tactggatta ttgaaatcac ctaggaaaaa aagtggtacg
    42661 cgagtttcca aaaagtagtt ttccaagttt ttccactcaa aataaaagcc acttttttta
    42721 tctgtccacc aagaaaagtg gcagctcgag acaaaaatgt cctgatcatc tatctttgtt
    42781 ttcccaatca agatcttgcg tgtctgataa tcttctttct tttgaaattg ggagacggta
    42841 aggacctgac tttccaatgt ttttttcgta agcagtgcat ttccttcttc atatttatca
    42901 taaccgatgt ggctcatttc ccaactccaa aaatactctt ttccgtgttc ctttaaaagc
    42961 tgaactaaac ggtaggcaaa attatcttta cggataggta cttgattctt taccggacag
    43021 aacttcatta gcttcgcagg ttctacttct tcagattcca gtaattggtt gatttcttgt
    43081 aaagcaatca cttcataatc ttctaacagg atcttatctg ctaattcttg caattttttc
    43141 tctggttctg gctcaagcca gctatgcgtg ttgagtgtca tgaatttcat tggttttctc
    43201 cttgtcttca tttcgaaaca aaagggttgt gacaaaattt ttgtcacaac ctctccagca
    43261 aatagtgttc tcctgctaac tcctcagtaa taagctcaat ggaccaggag ctattttttg
    43321 tttggctgtt tttatcactt ggtgcagggc agctttttca cgacctctcc actaaacagt
    43381 attcaaactg ctgcttctcg gtaataagcc caaccaaacg aaaaatagga ggagctattt
    43441 ttgtttggct gttcttatga cttgatgcag gacagctttt tcacaacctc tccttacatt
    43501 ttaatggagc cgatcacttt accagctgtc actgtaccta attgcttgat cactacttct
    43561 tctactttat cgtttgtaaa tacgatcatg atcgttggat ctttttcttc gttttggatc
    43621 acatcaagat ccatctcagc taatttcgtt cctgttccta cttcttggcc ttctttgaca
    43681 gcaatctcaa aagctggctg attcatttgg actgtatcga ttcccatatg gatcaaaact
    43741 tcgataccat ctttcgtttc cagcccaatt gcatgtttgc tagggaaaat acttgtcact
    43801 tttccagcga tcggcgcaac aacttctctt gatgttggaa taacagcaaa gccgtcaccc
    43861 atcatttttt gtgagaatac cggatctttt acttcagata aaggaatcac ttctccagaa
    43921 gctgcggaaa ctagttcttg ttctatacct aagtattgct tactacctgc ttgtccattt
    43981 cctgtagaaa ccatttctgg ctctggaatc tctgctcctg attgcagcag atcttcgata
    44041 tctgatttca agacatctgc ttttggtcca taaactgctt ggactccatt gtctttgacg
    44101 atcaatccca tcgcccctgc tcgtttccag gcttcttcag tccctacttt ttctcgatca
    44161 tttacactta cgcgtaaccg agtcatacaa gcatcgacat ctttgatatt ttcttttcct
    44221 cccagcaaat gaatgatctg gaccacttgc tgatctgctg ttcctgttcc gctgttagat
    44281 acaacggttt cttcactgtc attgtcgtaa tttccgttac gtcccggtgt agcaaagttg
    44341 aattttttga tcaagaaatt tgctaagaaa tacgtaacaa caccaaaagc gatcacacac
    44401 aacacgaagt tgataagatc tccacctaga cccgctttga tagctaacgg tgtccgagtc
    44461 agcaattcga tattcccaaa tgaatggacc cgcaatggca agatatctgc catcgcaaat
    44521 gcagcccctt ggatcactga ataaatgaca tataatggca cagcagcaaa cataaacatg
    44581 aactccaatg gttctgttac acctgttaga aagacagcca acgctgctga aaaatacatt
    44641 gatttatatc ttgctttctt atcaggatcg acattacgat acatcgcaaa agccattccc
    44701 atcaagatac ctgaagaacc aatcatttgt cctactttga aacgagccgg cgtccagtta
    44761 tgcaacacaa actcatattg cgacatatcc cctgcacctt taagattcac taaatcagtg
    44821 gcccaagcca accataacgg atcttgccca aatacttgtg tccccgcctg tgcgcctgat
    44881 aaaatctcat acgatccgcc aagctgtgta tagttgatag gaatcgtcag catatgatgc
    44941 aatccaaatg gcaatagtaa acgttctaat gttccaaata aaaacggtgc tagaatcggt
    45001 gcgctttctt gtgattctgc aatccataaa ccaaaattat tgattccagc ctgtacgttt
    45061 ggccacacca cagcaagaat caatgccacg attgttgacc ataagataac aacaaacgga
    45121 acaaagcgct ttccattgaa aaatgacaaa gcatccggca attttctata attatagtac
    45181 ttattataag ccattgcccc gacaaatcca gcgatgatcc ctacgaatac tcccatattc
    45241 aaagctggtg cttccaatac acttgtaaaa aatcctttaa ccatgatctt cgtaccgaac
    45301 agagtgtggg taaatgcatc ttcattcgca agcatgtcac ttgtgacacc aaagatagcc
    45361 ccagtgatcc gattgattaa aataaatgag atcccagctg caaatgctcc gcctgctctt
    45421 tcttttgccc aacttcctcc tattgctaaa gcaaataaaa tatgaagatt accaataatt
    45481 gcccaaccaa tattttcaat tactccgccc gttgtgacaa gcggcgtcca atcaggattg
    45541 atcaagggaa ttgatttccc tatactgatc atcaacccag ctgccggcat aactgctacc
    45601 acaaccatta aagcctttcc aaatttttgc caaaactcaa aactaaatat ctttttcatt
    45661 ccgtttagct cctttacgca atcggtttcg ttaaacgtat tatagcgtgc gttttctttt
    45721 ttgtaaatgc tttatttttt atttttctgt ctataccatt ctataaaacc tataatacgt
    45781 gtgttccctt ctataatttt ctcctaaaaa tttatttttt taagcttttt tctatgaaat
    45841 attttctttt gtctattgac ttgtgcaatc ggttgcgttt attatgaacg tataaaacag
    45901 ctcattagga ggagaaaaat gaaacagatc aaacgactat ttgctattga tccatggaaa
    45961 atcagcacga cacaattaga taaaaaaaat cttcgactgc aagaatcttt aacaagtatt
    46021 ggaaacggtt atatggggat gcgcgggaac tttgaagaaa cttactcagg ggaccaccat
    46081 caaggtactt atttggcggg tgtctggtat cccgataaga cacgtgtcgg ctggtggaaa
    46141 aatggttatc cagaatactt tggaaaagtg atcaacgcct tgaactttat cgcgatagat
    46201 atttcgatca acgatcagct gatcgacttg gcttctttgg aaccagaaga cttttactgg
    46261 gaattagata tgaaaaatgg tgtattatct cgtacattca caatcactac ttctactaat
    46321 aaagtacgca tttccttcga acgttttctg agcattgtca aaaaagaatc agcttatatc
    46381 tatttaacta tagaaatgct tgaaggtagc ggaacagtca aactcgtatc aaaattggat
    46441 ggaaatgttc aaaacgaaga cagcaactat gatgaacatt tttggaatga gattgatcgt
    46501 ggaactatag aagaaatcgg ctatttgacg actaaaacta ttccaaacaa ctttgggatt
    46561 gaacaattta ctgttactgc agcaatgcgc catttcgtta atggaaaatc agtcatccct
    46621 gactatcacg aagatacttt agcattacat ggttgcttgt ctgcagaatt atctcaaggt
    46681 gagacatggt cagtggaaaa agaaattatc gttcttacta gtagagatat tccagaagcg
    46741 cagcaagcag aaaaagctgt ctcagaatta acgaaccaga caaacaatta tatccaagca
    46801 aaagaagagc aaacagcggc ttgggcagaa cgatggaagc ttgccgatgt ggtcatcgaa
    46861 ggagacgaag aagcacaaca aggtattcga ttcaatttgt tccaactatt ctctacttac
    46921 tacggcgaag atgaacgatt gaatatcggc cccaaaggat tcacaggaga aaaatacggc
    46981 ggagccactt actgggatac agaagcgtat gctgtccctc tttatcttgc tttagccaaa
    47041 cctgaagtaa ctaagaatct gctgaaatat cggcataatc aactgccgca agcaatccac
    47101 aacgctcagc aacaaggatt aaaaggcgcg ctttatccta tggtcacctt cactggtgtc
    47161 gaatgccata acgagtggga aatcacattc gaggagattc accgaaatgg cgcgattgcc
    47221 tatgcaatct acaattatac gaattacacc ggcgatacgg aatatttgaa aaatgaagga
    47281 ctggaagttt tagcagaaat cgcacgcttc tgggcagacc gtgtccattt ttcaaaacga
    47341 gcaaataaat acatgatcca cggtgtgacc ggcccaaacg aatatgagaa caatatcaat
    47401 aacaattggt atacaaatac gatcgcagct tgggtcttgc gttacacgcg agaaaattat
    47461 ttaacttatc aaaaagaagc tgctatcact atcagcgaag aagaactagc caaatggaaa
    47521 gatatcgcag agaacatgta cttcccagtt gacgaagaat taggaatctt tgtccagcat
    47581 gacacattct tagataaaga tctcatgcct gtatccgata ttcccacaag cgatcttcct
    47641 ctgaatcagc attggtcttg ggacaaaatc cttagatctt gcttcatcaa gcaggcagat
    47701 gtactacagg ggatttattt cttcaatgat acttttacaa aagaagaaaa gcaaaaaaac
    47761 tttgatttct atgaaccaat gactgtccat gagtcttccc tttcgccaag tatccattcg
    47821 attttagcag cagaattagg aatggaaagc aaagctgtag aaatgtacca gcgaacagct
    47881 cgcttggatt tggataatta caataacgac acagaagacg gcttgcacat cacttccatg
    47941 actggtagct ggttgtcgat tgtccaagga tttgctcaaa tgaaaactgc ccatgatcag
    48001 ttgagattcg caccatttct gccaaaaact tggaaagctt attctttcca tatcaattat
    48061 cgcggacgtt tattgttcat ctcagtcaca gatgaacaag ttactttcga gttattagaa
    48121 ggttctcctc tagaaattta cgtctatgaa cagtcttatt ctttagcgga tcgtctagaa
    48181 ataacgctaa gaaaggagga agcaaatgtt taaaggggta ttgtttgatt tagacggtgt
    48241 cattaccgat actgctgaat tccattatcg tgcttggcga aagcttggtc aggaaatcgg
    48301 cattacgatc gaccacacat tcaacgaaca gctaaaaggc gtcagccgag aagactcact
    48361 ctctttgctt ttagcttatg gcggaaaaga acactctttt tcaaaggaag aatttgcaga
    48421 attagcgaaa agaaaaaacg attattattt agagatgatc cagacgatcg aaccaaaaga
    48481 tgtcttcccc ggggtcgtgc ctttactgga ttctttaaaa gaagaaaaaa tcaagattgc
    48541 tcttgcttct gcaagtaaaa acgggccatt cctacttgaa aaaatgggtc ttacacctta
    48601 ctttgatgct attgccgacc cggcagaagc aacaaacgga aaacctgcgc cagatatctt
    48661 cctccttgca gcaaaagctg tcggactaaa agcagaagaa tgtatcggga tcgaagacgc
    48721 gcaagccggt atccaagcca tcctctcaag tggcgctcaa ccaattggcg tgggacgtgc
    48781 tgaagacctt ggagaggaga tcccgcttgt ccctgatacg acttttctta cgatcgatta
    48841 cttgaaagaa aagtggcatg accatggata atccagagat ctccgtcgta ccagttccag
    48901 agactgaaat aaagaaaatc acattgaaga acgactactt agaagtcgtt cttctcaatt
    48961 acggtgcccg cctgcaccaa atcttcgcac cagacaaaga aggaaaatca gaaaatgttt
    49021 tattgtctta cgatcgtttc gaagatgttc taacagacaa aagctttttt ggagcaactg
    49081 tcggacctgt agccggaagg atccgggatg cttcttgggg tgagcataga ttggagaaaa
    49141 actgcggatc acaccatatc cacggtggca cgaaaggctg gtctttccaa ttttgggaag
    49201 tagaagtttt caaaactcca tcatcaatag gtgtggtctt ttacttaaaa gatgaatttt
    49261 ctacctatcc aggaccaatc actgctacga tcacttaccg tttaacaaaa aacgaattag
    49321 agatgacaac ctctgctagt agccttgttg aaaccatctg caatccgacc aaccatgcct
    49381 acttcaacct ttcaggaaat ggaacaagag acatctatga acatcagtta acggtctttt
    49441 tagatggtgt gcttgaatta gatcaagaaa aactgcctac tggaaattgg acaaaaaaag
    49501 aaaaactgcc aatcgacttt agaaaatccc caacactgca agagatcctt gcatgttatc
    49561 cagatggact agatgatgta tttcttttgc atcatcctag gctttccaaa acttccttac
    49621 aattattcga gaagcattct ggtcgccaaa tgactatagc aacttctaat aaaagtatgg
    49681 tattattttc tacgactgga ttcgaagctg atttctctgt caatggaaaa cagatgcatt
    49741 caaactacgg attagcaatc gaaccgcaag aaatccctga tatcgttcat ttccccaaat
    49801 ttggatcgat caacctccat cctggtcaag aacggatcag tcagacgatt tatcggttct
    49861 ctgcacaata acaagagaaa ggtgttttta tcatggctat taccgtaaaa gatgtggcga
    49921 aaaaagccgg tgttgccacc tcgaccgtct ctcgtgtcat caacgatcat cctagtattt
    49981 ccgagacaac aaagaagaaa gtccgaaaaa ttatggatga actaggttat gttcctaaca
    50041 tgactgcccg caatctagga aaacgcattt ccagtgccat cggcgtaatc ttaccccctc
    50101 ttgattcaaa agagcggatc ggtaacccat tttatcttga gatcatggaa gcaatcaacg
    50161 aagaagcgcg gaattatgat atgactacag caatcgctac tgctaaaaat tttgaattgc
    50221 tgttagagaa cgtccagcgt atgcatttac aaaaacaagt agatggtttt attctagtgt
    50281 attcagatag tcaagatact gtcattgatt atttatacca aaaccacatc cctttcactt
    50341 tgatcggaca gccttatcat catgagaacg aaatcgttta tgtagataat gataaccagc
    50401 ttctaggtaa acaagcaaca gaatttttga tccagaatgg acataagaat atcctgtttg
    50461 tgacaaacac gactcatgaa agtttgtatt tcgaacgata tttcggttat caaaaagcaa
    50521 tgatgatggc tggtcttcct gcgttatcca gtgtagtact agagcatccg gaggattatc
    50581 tgtcctttga gaaaacgttg aaagaaaacg aagccacagc attggtggtc attgacgata
    50641 tcttcgccct tcgcacgatg cagttggcgc aaatgtatgg ctatgtcatt ccagagacac
    50701 tctccgtcat tagcttcaac aactcgatct tctcaacatt aacacaccct taccttacaa
    50761 gtatcgatat cgatatctcc gaattaggac gtgtcgctat gcaaaagctg cgcgaattga
    50821 tccatgaaga attgagcagt ggcgtacgtt tggtcatccc tcatcgcttg atcaaaagag
    50881 agacagtcgt tcctttagaa aacccatact aaatagctga ctcactaaat acgctgacca
    50941 tttatcctat ggtcagcttt ttatgtaaaa gaagatacta cttctaacac attgtttatc
    51001 tgtaaaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta
    51061 taaacggcgg aagcagaagc aactccttcg gaaataagct gaaattcaca aaaatttaaa
    51121 aagcaatttt cttgagtttc ttcttatttc ttggagttaa acgcttctgt cccagcctct
    51181 tttttccttc cttcaatttt ttacacgtcg acgattcaat aaaaaaccgc ctaatattgt
    51241 aaagacaggt gcaagaatcg agaaaaaggc gaatggcaag taatgcaacg cgctaacttt
    51301 catcgttccc ataatgaatg tgccgctgac tccccacggt acgagagaat tgacggtcgc
    51361 acctgaatcc gctagtgttc gtgtcaaata ggtttttgat aagccaacgt catcaaaaga
    51421 acgcttgaat gtttctcctg gaagaatgat ggacagatac tgttctccaa cgatcagatt
    51481 gattccgata cagctcaagg cagtaaaccc gactaaacga gaaggtcggt ccatcttttc
    51541 ttttaattct tgaacgatgg tatcaacgat cagatatttg atcagtaacc cacctaatgc
    51601 caatgcaagt aaaatcaaac tagcagaacc taacatactc atgatcccgc ctcgagaaag
    51661 cagttcatct agctttggat caggtgtatt tgcgacataa ccattcatca agatgtctgc
    51721 tgcttttccg accgtcaaac ttttctcatg gataaaagaa agaacgagag cagctgtcga
    51781 tcccgctagt aatgtaggga tagccggaat ttttttgatt gcccccccaa ataaaataac
    51841 tagtggaatc aatgtccaag gagagatcca aaagctcgct ttcatgtttt ggatcatttc
    51901 attgatacta gctagatcgg ctgaatgact gttatgtcct aatatcgtat aaaaaatcaa
    51961 cgaaaggacg aatgctggga aatccgtata acgcatacca gcgatatgtt cgaatagatt
    52021 aactcctcca atacccgctg ccaagttggt cgtatctgat aaaggcgagc agttattccc
    52081 taaaaaggca ccggaaacga ctgcgcctgt agtcattgca tcattgaatc caagtaaatg
    52141 accgatcccc aaaaaagcaa ttccaatcgt cgaaatcgtt gtaaatgaac tgccgacagt
    52201 cactccgaca attgcacaaa caagaaatgc tgtcggtaaa aagaaacgca cagaaacaag
    52261 tttaaatcca taaaccataa tcgtagggat ggttccagat aaaatccatg aagcaactaa
    52321 gacaccaatc aaaagaaaaa tcaggattgg aatgatccct ggagtgattc ctttgatgat
    52381 cccgtcatgg atctcatccc atgtgaactt tttaaatctg ccataaaaca acaaacacat
    52441 gaaagctgca aggatcggga tatgtggcga gagctggaat ccgatgatca gtactcctag
    52501 gagtgccaac aacacgatca atacagcgat actttctttt aaagaaatat ttttattcat
    52561 cgtcgatctc ctatttcaaa tgaaaaggcc ctgccatgaa aaaactattc acttggcagg
    52621 acctcctact aaatcagcga caaaacgtcg gctgaaagac ttattttttt atttttcttc
    52681 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52741 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52801 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52861 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52921 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    52981 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53041 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53101 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53161 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53221 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53281 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53341 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53401 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53461 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53521 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53581 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53641 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53701 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53761 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53821 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53881 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    53941 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54001 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54061 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54121 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54181 gggattcttt gagtttattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54241 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54301 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54361 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54421 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54481 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54541 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54601 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54661 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54721 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54781 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54841 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54901 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    54961 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55021 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55081 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55141 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55201 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55261 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55321 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55381 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55441 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55501 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55561 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55621 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55681 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55741 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55801 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55861 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55921 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    55981 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56041 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56101 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56161 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56221 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56281 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56341 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56401 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56461 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56521 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56581 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56641 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56701 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56761 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56821 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56881 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    56941 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57001 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57061 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57121 cttctgcttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57181 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57241 cgatagattg caggatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57301 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57361 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57421 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57481 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57541 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57601 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57661 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57721 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57781 tttatgaaag caggaacctg ctagcatatg taattaatta tttcttcagg ataggatagg
    57841 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57901 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    57961 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58021 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58081 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58141 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58201 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58261 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58321 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58381 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58441 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58501 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58561 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58621 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58681 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58741 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58801 tgattttgtt ttagttaatt tgaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58861 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58921 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    58981 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59041 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59101 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59161 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59221 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59281 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59341 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59401 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59461 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59521 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59581 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59641 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59701 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59761 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59821 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59881 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    59941 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60001 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60061 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60121 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60181 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60241 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60301 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60361 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60421 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
    60481 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
    60541 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
    60601 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
    60661 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
    60721 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
    60781 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
    60841 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
    60901 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
    60961 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
    61021 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
    61081 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
    61141 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca ctcgatgaag aaggaacact
    61201 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
    61261 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
    61321 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
    61381 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
    61441 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
    61501 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
    61561 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
    61621 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
    61681 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
    61741 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
    61801 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
    61861 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
    61921 agcggattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
    61981 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
    62041 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
    62101 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
    62161 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
    62221 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
    62281 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
    62341 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
    62401 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
    62461 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtag tcttgatttg
    62521 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
    62581 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
    62641 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
    62701 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
    62761 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
    62821 atggcttcct cgaatcggta aacttgctta tatctactta gcttactcct ttgcactcaa
    62881 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
    62941 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
    63001 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
    63061 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
    63121 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
    63181 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
    63241 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
    63301 tgatccttcc ctgtcgcttt agctaagtca agcagataga gagcgactcg ttcttctact
    63361 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
    63421 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
    63481 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
    63541 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
    63601 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
    63661 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
    63721 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
    63781 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
    63841 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
    63901 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
    63961 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
    64021 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
    64081 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
    64141 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
    64201 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
    64261 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
    64321 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
    64381 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
    64441 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
    64501 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
    64561 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
    64621 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
    64681 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
    64741 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
    64801 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
    64861 aactgcgatg agcatcaccg cagtttt
//
LOCUS       DANJMI010000012        63208 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000012 DANJMI010000000
VERSION     DANJMI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.012"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(151..2304)
                     /locus_tag="PWU81_000910"
     CDS             complement(151..2304)
                     /locus_tag="PWU81_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL1109636.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2598..3446)
                     /locus_tag="PWU81_000911"
     CDS             complement(2598..3446)
                     /locus_tag="PWU81_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL1109637.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="PWU81_000912"
     CDS             complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="PWU81_000912"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL1109638.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5947..6126)
                     /locus_tag="PWU81_000913"
     CDS             complement(5947..6126)
                     /locus_tag="PWU81_000913"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDL1109639.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="PWU81_000914"
     CDS             complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="PWU81_000914"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL1109640.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8274..10553
                     /locus_tag="PWU81_000915"
     CDS             8274..10553
                     /locus_tag="PWU81_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1109641.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10646..11293)
                     /locus_tag="PWU81_000916"
     CDS             complement(10646..11293)
                     /locus_tag="PWU81_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109642.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11472..12191)
                     /locus_tag="PWU81_000917"
     CDS             complement(11472..12191)
                     /locus_tag="PWU81_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1109643.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12322..13473)
                     /locus_tag="PWU81_000918"
     CDS             complement(12322..13473)
                     /locus_tag="PWU81_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDL1109644.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13690..14430)
                     /locus_tag="PWU81_000919"
     CDS             complement(13690..14430)
                     /locus_tag="PWU81_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL1109645.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="PWU81_000920"
     CDS             complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="PWU81_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL1109646.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14814..15407)
                     /gene="yqeK"
                     /locus_tag="PWU81_000921"
     CDS             complement(14814..15407)
                     /gene="yqeK"
                     /locus_tag="PWU81_000921"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDL1109647.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15391..16041)
                     /locus_tag="PWU81_000922"
     CDS             complement(15391..16041)
                     /locus_tag="PWU81_000922"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDL1109648.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="PWU81_000923"
     CDS             complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="PWU81_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL1109649.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="PWU81_000924"
     CDS             complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="PWU81_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDL1109650.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17484..18011)
                     /locus_tag="PWU81_000925"
     CDS             complement(17484..18011)
                     /locus_tag="PWU81_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDL1109651.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18191..18976)
                     /locus_tag="PWU81_000926"
     CDS             complement(18191..18976)
                     /locus_tag="PWU81_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL1109652.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18969..19838)
                     /locus_tag="PWU81_000927"
     CDS             complement(18969..19838)
                     /locus_tag="PWU81_000927"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDL1109653.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19839..21218)
                     /locus_tag="PWU81_000928"
     CDS             complement(19839..21218)
                     /locus_tag="PWU81_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL1109654.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="PWU81_000929"
     CDS             complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="PWU81_000929"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL1109655.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21670..22146)
                     /gene="accB"
                     /locus_tag="PWU81_000930"
     CDS             complement(21670..22146)
                     /gene="accB"
                     /locus_tag="PWU81_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL1109656.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="PWU81_000931"
     CDS             complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="PWU81_000931"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDL1109657.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="PWU81_000932"
     CDS             complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="PWU81_000932"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDL1109658.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="PWU81_000933"
     CDS             complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="PWU81_000933"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL1109659.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25176..25400)
                     /locus_tag="PWU81_000934"
     CDS             complement(25176..25400)
                     /locus_tag="PWU81_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL1109660.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25453..26415)
                     /locus_tag="PWU81_000935"
     CDS             complement(25453..26415)
                     /locus_tag="PWU81_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL1109661.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26425..26865)
                     /locus_tag="PWU81_000936"
     CDS             complement(26425..26865)
                     /locus_tag="PWU81_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL1109662.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27171..28136
                     /locus_tag="PWU81_000937"
     CDS             27171..28136
                     /locus_tag="PWU81_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL1109663.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28193..28423)
                     /locus_tag="PWU81_000938"
     CDS             complement(28193..28423)
                     /locus_tag="PWU81_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HDL1109664.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28509..29396)
                     /locus_tag="PWU81_000939"
     CDS             complement(28509..29396)
                     /locus_tag="PWU81_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL1109665.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29485..30708)
                     /locus_tag="PWU81_000940"
     CDS             complement(29485..30708)
                     /locus_tag="PWU81_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL1109666.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30725..31771)
                     /locus_tag="PWU81_000941"
     CDS             complement(30725..31771)
                     /locus_tag="PWU81_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL1109667.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(31935..33662)
                     /gene="ptsP"
                     /locus_tag="PWU81_000942"
     CDS             complement(31935..33662)
                     /gene="ptsP"
                     /locus_tag="PWU81_000942"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDL1109668.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(33662..33928)
                     /locus_tag="PWU81_000943"
     CDS             complement(33662..33928)
                     /locus_tag="PWU81_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDL1109669.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34407..36641
                     /locus_tag="PWU81_000944"
     CDS             34407..36641
                     /locus_tag="PWU81_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1109670.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36789..37109
                     /locus_tag="PWU81_000945"
     CDS             36789..37109
                     /locus_tag="PWU81_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL1109671.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(37314..37538)
                     /locus_tag="PWU81_000946"
     CDS             complement(37314..37538)
                     /locus_tag="PWU81_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HDL1109672.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(37736..39316)
                     /locus_tag="PWU81_000947"
     CDS             complement(37736..39316)
                     /locus_tag="PWU81_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL1109673.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(39717..41093)
                     /locus_tag="PWU81_000948"
     CDS             complement(39717..41093)
                     /locus_tag="PWU81_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL1109674.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(41221..42339)
                     /locus_tag="PWU81_000949"
     CDS             complement(41221..42339)
                     /locus_tag="PWU81_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL1109675.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            42481..42915
                     /locus_tag="PWU81_000950"
     CDS             42481..42915
                     /locus_tag="PWU81_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1109676.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(42993..43526)
                     /locus_tag="PWU81_000951"
     CDS             complement(42993..43526)
                     /locus_tag="PWU81_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDL1109677.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(43620..44798)
                     /gene="mutY"
                     /locus_tag="PWU81_000952"
     CDS             complement(43620..44798)
                     /gene="mutY"
                     /locus_tag="PWU81_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL1109678.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(44791..45588)
                     /gene="recX"
                     /locus_tag="PWU81_000953"
     CDS             complement(44791..45588)
                     /gene="recX"
                     /locus_tag="PWU81_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL1109679.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            45695..47077
                     /gene="rlmD"
                     /locus_tag="PWU81_000954"
     CDS             45695..47077
                     /gene="rlmD"
                     /locus_tag="PWU81_000954"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL1109680.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(47251..47829)
                     /locus_tag="PWU81_000955"
     CDS             complement(47251..47829)
                     /locus_tag="PWU81_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HDL1109681.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            48151..48621
                     /locus_tag="PWU81_000956"
     CDS             48151..48621
                     /locus_tag="PWU81_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDL1109682.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(48729..49955)
                     /locus_tag="PWU81_000957"
     CDS             complement(48729..49955)
                     /locus_tag="PWU81_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL1109683.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(50709..50909)
                     /locus_tag="PWU81_000958"
     CDS             complement(50709..50909)
                     /locus_tag="PWU81_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL1109684.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(51043..51651)
                     /locus_tag="PWU81_000959"
     CDS             complement(51043..51651)
                     /locus_tag="PWU81_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDL1109685.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(51736..52206)
                     /locus_tag="PWU81_000960"
     CDS             complement(51736..52206)
                     /locus_tag="PWU81_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDL1109686.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(52254..53168)
                     /locus_tag="PWU81_000961"
     CDS             complement(52254..53168)
                     /locus_tag="PWU81_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL1109687.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            53314..54117
                     /locus_tag="PWU81_000962"
     CDS             53314..54117
                     /locus_tag="PWU81_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HDL1109688.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            54295..55242
                     /locus_tag="PWU81_000963"
     CDS             54295..55242
                     /locus_tag="PWU81_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL1109689.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(55310..55603)
                     /locus_tag="PWU81_000964"
     CDS             complement(55310..55603)
                     /locus_tag="PWU81_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HDL1109690.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(55631..56296)
                     /locus_tag="PWU81_000965"
     CDS             complement(55631..56296)
                     /locus_tag="PWU81_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDL1109691.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(56436..57068)
                     /locus_tag="PWU81_000966"
     CDS             complement(56436..57068)
                     /locus_tag="PWU81_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1109692.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(57075..58142)
                     /locus_tag="PWU81_000967"
     CDS             complement(57075..58142)
                     /locus_tag="PWU81_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL1109693.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(58139..58870)
                     /locus_tag="PWU81_000968"
     CDS             complement(58139..58870)
                     /locus_tag="PWU81_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDL1109694.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(59039..59518)
                     /gene="greA"
                     /locus_tag="PWU81_000969"
     CDS             complement(59039..59518)
                     /gene="greA"
                     /locus_tag="PWU81_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL1109695.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(59654..60829)
                     /gene="mltG"
                     /locus_tag="PWU81_000970"
     CDS             complement(59654..60829)
                     /gene="mltG"
                     /locus_tag="PWU81_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL1109696.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            61033..62202
                     /locus_tag="PWU81_000971"
     CDS             61033..62202
                     /locus_tag="PWU81_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL1109697.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(62469..>63208)
                     /locus_tag="PWU81_000972"
     CDS             complement(62469..>63208)
                     /locus_tag="PWU81_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109698.1"
                     /translation="KLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRFM
                     IFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQGT
                     YSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTFK
                     SSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFSK
                     ISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT        18333 a        13231 c        10648 g        20996 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aaccaagaag tatttaatat
     2281 ttttttcata aaatctttca tcatttccta tccttccgcc tatttacatt catgcttcat
     2341 tgtacccaaa aacttcttaa taaaaaagaa aaaatgggcg gagcaagaaa ttctttacct
     2401 aatttcaaga taaaatcaac tttttgtttc acagcaatca cttaaagtta catatccaac
     2461 tatttttttc attaaaaaat aaacttattt ttgaaaatta tgttttctta agtcgtgaaa
     2521 cactttgatg gatcatttat ttgtggaaaa ctacgttttg ttattctaaa aaacttatat
     2581 aaaaaatagt aaggttttta tttcgaatcc ataaaatatt cttggattgc ttctcgtgtt
     2641 tttttcactt tttcgctaag cttttcgttt ttagccatat agaaataaaa aatgatatcg
     2701 atcgtagcaa attgggccaa aagtgaatta gttgccgcac ttcgattcgt gatttctttt
     2761 ggtctagatg tttgtacgag tacatcggct atcttgctaa gcgggttatt tccaaattgc
     2821 gtcaacgcaa tgatttcgat attcatgctt ttcgccagct cggctaatgc gactacatct
     2881 gcacttctac ctgagtttga aacaagccaa agcactttct ttttctcatt gctgacaagt
     2941 tgcgggagca agacgtggat atctttttca aaaatgattg gcttaccgac acgagaccat
     3001 ttttgcagaa tatcttctac tactaaagaa gaggcgccaa ccccatataa aaaaacagtc
     3061 tctgccgatt ccaaacactc tacgactttt tggatcgttt cttcttctag aatatctact
     3121 gtttctttta tcgctacttg tgcattgtta ccaagtttgt ttttgataga gtaaaaggac
     3181 tcatttgctt ctatatcact aaaaccttca tcgattccag tctgtagttc tgttgaaatc
     3241 gtgattttga agtccgtcag actttgaaaa ccgatttttt tggaaaaacg tacaactgtc
     3301 ggcgcactca acccggaagc ttctgctaat tcattagctg tcattgtagg gaccttctca
     3361 atattaccga taatgtagtt ggccaacttt ttttctgctg aagaataatc ttccatcata
     3421 ctttcaatac gactaattac gctcataagt tcctccaccc ttttaatgat agtctatgta
     3481 agtataccat atcagaccct tattcacggt cgtatactgg ataaaaaaca aaaaaacaat
     3541 ccgcccaaca gaatatctta tcccatcaat cccacagatt ctattgagca tctgtgtctt
     3601 tgaggaatac ctgttatcta ttgttcggat tgctttctat ctactacttt tttacaggaa
     3661 atttacctat tagttttctc tgctgtaatt tttgacttct gcttccatag catcaataaa
     3721 gatattcaaa tcttctacag ctgtttcttt acttccataa cgacgtacgt tcactgttgc
     3781 atcttccaat tccttgtctc caacaactaa ttggtaaggg atcttttgtg tttgagaggc
     3841 acgtatctta tagcccattt tttcattacg atcatcaact tctacacgca accccttcat
     3901 ttgcaaacgt tctttgattt cataagcata atcgccatgt gcatcaacag aaacagggat
     3961 gattgttgct tgtataggtg ccaaccatgt tggaaaagcg cctttgtata cttctgttaa
     4021 ataagcgaca aaacgttcca ttgtagaaac aattccgcgg tggataacta ctggacgatg
     4081 cgtattttcc ccgtcttctc caacatatgt caaatcaaag cgctctggca ataagaagtc
     4141 taattgtata gttgataatg tttcttctgt accaagagct gttttgactt gaacatctag
     4201 ttttggtccg taaaaggctg cctcgccttc tgcttcaaag taatctagtt ctaattcatc
     4261 catagcggct ttcagcattg cttgggcttt ttcccacatt gcgtcatcat caaaatattt
     4321 atctgtattg tttggatcgc gatagctcaa gcggaaacga taatctgtaa tattgaagtc
     4381 agcatatact gccaccatca attcaagcgt acgtttaaat tcatctttga tttgatcagg
     4441 acgaacaaat gtatgaccat cattcaacgt catttctcgt acacgttgta aacctgacaa
     4501 agcacctgat ttttcatagc gatgcatcat tcctaattca gcgatacgaa ttggtaattc
     4561 acgataagaa tggatcgtgt ttttgtagac catcatgtga tgagggcagt tcattgggcg
     4621 aagaacaagc atttcgccat ctcccatatc cattggcggg aacatgtctt cttgataatg
     4681 atcccagtgt ccagatgttt tgtacaactc aacatctccc attattggtg tatatacatg
     4741 ttggtaacct agactaactt ctttgtctac gatataacgt tcgatcgtgc gtcggattgt
     4801 tgcccctttt ggaagccaga atggcaaacc agaacctact tctggggaaa ccatgaacaa
     4861 gtcaagttct tttcctagtt tacgatggtc tctttctttt gcttcttcac ggagacggat
     4921 atattctttc aaagctttct tgtcaaaaaa ggctgtcccg tatacacgtt gcatcatttg
     4981 attgtttgag tttccacgcc aataagcacc tgccactgat aataatttaa atacttgtat
     5041 acggccagtg gatggaacat gtggtccacg acacaaatcg atgaattcgc cttgttgata
     5101 agcagtaatc acttcttctt ctggcaattc actaatcagt tctactttgt acggatcact
     5161 tgcaaaaatt tctaatgctt cctgttttga aagaacacgg cgttcgatcg gcagattttc
     5221 tttgacgatt ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag ttacttggtt
     5281 ttgtccatta tctgtatcgt agtaaaaccc tgaatcgata gctggtccaa cgccaaagtg
     5341 gatatttgga tagaggcgac gcatggcttg ggccatcaaa tgagctgctg aatgacggac
     5401 taagccaagt gcatcttcat ggtctggtgt gatgatctca atacttgcat cttcctcgat
     5461 tggacgagtc aaatcaacga gttttccgtt cactttacca gctaaagctt tttttgctaa
     5521 gctttttgaa atgctttcag caattgccaa tgttgttgtt ccagactcaa attctttgac
     5581 tgcaccatct ggaaaagtaa tttttatcat aatagtttcc tccttgaaat gttttgattg
     5641 atcagatcat caagctgaaa aacaaaaaaa tcctagcatc tcttgaaaag agatactagg
     5701 acgaaaagtt cgtggttcca cctaatgttc aaacgcaaat gcgttcctca agcgttgtaa
     5761 cggtccgacc gcccttgctt caacaagggt ttcgaaagtg gtcatccttt ggttttctcc
     5821 tggaaaagtt tcagcctagc ttttcctctc ttatcgaaga ctctcaaatt cagttggctt
     5881 tctcatcaat caattatgtg tttatttaac cactaataaa tagaaaaatc aagtgggaaa
     5941 ctaaacttat tttttccatt ccccatttac accgtatgtt ccttttttca tctcattgat
     6001 gatgacatgg atgtgttctt ttggtgcctg cgtgttttta tgaacagctt ccgtgatatc
     6061 tttcatcata ttttctaatt gttccggact gcgaccttct actaattcga catgtacgaa
     6121 tggcataatg ctctctcctc tttctgatcg ttcttttttc aactcgattc tatcatacta
     6181 tgtttgtctt gggtactgaa tcaggaaaaa tggctggatc tttttttgct ctcaaggcac
     6241 tcaaaataga gaccgtatcc acgacttctt gcaaaacagc tccgaataat gcggggatta
     6301 gtcccgtact tgcaattaac atcaaaaaaa tacaaataaa gatgccaatc agtacagact
     6361 gtttcgctat tctcatcgta tctcttgaaa tcactactgc ttttgctaca cgcaagagat
     6421 catctttcaa gatgaccgca tctgcacttt cactagcagc tgtagaacca tgagccccca
     6481 ttgcaatacc gatatctgca gcagcaagtg ctggtgcgtc attcacgccg tctcctacca
     6541 tcgcaacagg tctttgatct ttagggatac tttctaaaat ggtcagttta tcttgaggca
     6601 agctacgggc atgtacagaa tctaattgga ctttttctgc aatcgtttga gcaatggcag
     6661 atccatcccc ggtgatcatc atcaaatttt tcacatcaag ttttcttagc tccttgattg
     6721 tctttgctgc ttctgggcga atctgatccg aaaataggat cgttcctgca aaagatcgat
     6781 caattgttac atagattgct gtattggacg tttctatttc ttcagctgaa acaaaagagg
     6841 cacgtccaat ctttacttca tgattattga ctactgcttg gattccttga ccggtcactt
     6901 ctttcagttg actaacagga tacagttggc tgtttttcac gttgttcact aacgaacgag
     6961 ctaagatatg gctagattcc tgttctgcac tagctgcata cagaagtagc tcttcttttt
     7021 ttattccatt gactggctga atcgcctcta cttccaatat ccctttggta agcgttcctg
     7081 ttttatcaaa agcaatcgtt tttgtacgtg cgagcttttc aatcgttgtt ccagttttta
     7141 caacgatccc gtttttactt gaacggctca ttcctgcaac taaagcaatg ggcgcagcta
     7201 gaatcagtgg acaaggtgat gccactacca gtacctcagc aaatcgtaaa gggtcttttg
     7261 agaaccacca agaaatacca ccgatcaaat aagcaatcaa cgtgaaaggg actgcataac
     7321 gatcagctaa acggacaaat cgggcaggct tttcttttga ttcttcgact agtttaataa
     7381 tcatttgata ctggctgtct gcggcacgtt tttcagcgat catttggatc gatccgtctc
     7441 catttaaaga ccctgacata atctgatctc caggtttctt ggtttccggg cgggactcac
     7501 ctgtcaaaga agattcatcg accgaagatt ctcctttggt cactcgacca tctgcgggta
     7561 caagttcgcc aggcttgact agtaactggt catcaatatt tatttcatcg attactatat
     7621 cctgcaattt tccttctttg aatcgatggg caattcttgg ggtgttatct aacaaagact
     7681 gcagttcttt acccgctcgt ttattcgcgt aatcctctag actatctccg cccgtaagca
     7741 tgatcaatat catcaaacta gcccagtatt ctttgacggc taatgttgca aggattgcgg
     7801 taatcgctaa tacatctacg ccatatcttc ctgaacgtaa cgtcttgatc atttccaaaa
     7861 acatcaagaa ggcgagaatc cctccagtaa atgcgacaat cgcaaatgca agggccggct
     7921 ggtgaaaaac aaattcactt aataaagcta tcccaccaat aagcaaagta agtaaaaatt
     7981 ttttgaaatg tgtcattgtt ttttctcctc ttttatgtga cttagagagg ttagtaataa
     8041 atttccagct gcctctttca tcttaagcat agtccttgac tagcactttt taaaacaata
     8101 ggccttaatt gaaaacaact atcactatca gcgtttccaa aattcatcag ttctacaaac
     8161 aaaaatattc agcaaggatt actttttcga ttcatcagtt aaaaagtacc ttttttcgaa
     8221 aaactatgct atacttttaa ttcgttattt acggcggaag gagcaaacat gcgatgaatg
     8281 agaaacaact agaacaaacg cacgtggatg aaacgatcga actaatccag atcgaacaaa
     8341 atatgctgga tacaaaacaa caagaattga ctcaggaaat gaatcattcg acaaaagaca
     8401 cagctaatca caagattcga gctggttcca atgaagcttt ttatgaatct gctgttgaat
     8461 atcgccaaca tgagcaggaa ttgcttttga aataccatac tcttgaagca cagcaaaaac
     8521 gcctgcaaac gttgtctgtc atgaaagata gtccttattt tgcccgtatc gactttaaag
     8581 aagaccgtga aacagaaaca ttatatctag gtatcgcttc tttaagagat ctggcagaag
     8641 aaacaatcgt tattgactgg cgcgcaccaa ttgccaatct ttattatgaa ggtgagattg
     8701 gtcccgcatt ttatgaaact gaaacagata gaatagatgt cgatctgcag ttgaaacgac
     8761 aatttaaaat ccaagaagga cgtattttat ctatggtaga tacgtcggaa gtaatcaatg
     8821 atgacttttt gctagagata ttggatgaat catcaagcaa ccatatgaaa aacatcgtct
     8881 ctacgataca aaaagcacaa aatgcaatca ttcgggacac aacaagtaaa gtaatgctga
     8941 ttgaaggaat cgccggaagt ggaaagacat ccgctttgct tcaacgtatt gcttttctgt
     9001 tgtatcataa tcgcaagtgg ctcgaggctg aaaacgttct tctattttca cccaaccatt
     9061 tgttttctga ttacatctcg actgtattgc cttcattagg tgaaagcggc gtgcctacac
     9121 agacattcaa aaattatttg gtgcagctgc tgccagagtt ttctttacta gatgaacagc
     9181 aacaagaaag tggttttttg ctaggggaaa aagatccgat acaagtgatg aaaagcggat
     9241 tgaccttagt cgatcagatt gatcgctata tccaaggcat cacaaactac ggtcccttgt
     9301 tccgtgatat gaaaataaat ggccgtacga tcctatcaaa ggaatcgatt cgccaatggt
     9361 ataaagaaac aaatgaacaa ttacctttac atcatagact ttctctatta caaacgaagc
     9421 tcctgaaaaa attaggcggt ctgcaaaaag acgaaacacg tcagcaatgg gtcaaagatc
     9481 tggcggaaga acagctccaa gaactatacg caacagatcc aaatcttgaa tatacagaac
     9541 aaaaggagcg ttcattacgt aaaaaactgg ccaaccaaat cgtacgtaaa agattccgtc
     9601 gaatccatcg agggatcatg aattatcaat ttgtcaatat aacgaaacaa tatctccact
     9661 ttctacaaac tgtgccactt tctcttctaa cacgacataa aatcagcgag accaggtggc
     9721 aggaacattt gacaactgtt cgtacacact tgcgtcaaag agaattgcgt caagaggatg
     9781 ctgtactatt ctttttactg atgcgccgaa catatccagt gacggttaca cacaaagctc
     9841 gctatatctt tattgatgaa atgcaggatt tcgcaccatc acaagtggca ttgctgcaag
     9901 ccttgttccc aaaagcaaat ttgactttct gtggagatct aaatcaaaat gtctttggaa
     9961 atgaaacgat cgttggatca ttggaagacc ttttcgtgga ccgtgaagtc actcgtttcc
    10021 aattaacgac aagctatcgc tccacaaaag aaatcactga ttttgctaat cattttattg
    10081 tgaaagaaca tcaagtagaa acaacagcaa gaaaaggaga ccttcctttg ctcatccaag
    10141 gaacaacaga tgatatcaaa ttgaattggt tagcagatac cattcgaacg actcagggaa
    10201 aagcccggta ttggcgtaca gcaatcatag ctaaaacaca agaagaatgc gaagctcttt
    10261 atgctcaact gccagacgag ttgaaacaac aagtccagct gattttaaat gagagtgact
    10321 ttatgaagcg ttccattatt ttacttccag cctttttagc aaaaggtctg gagtttgacc
    10381 gagttttctt gtgggatgta gatgcagaac atttttcttc tcagcaagac aaattgatcc
    10441 tttacaccat gtgtacacgt gcgatgcatg aattgaccct ttttacctcc aataatgaaa
    10501 gtcctttgtt aaacaatatg gatatttcat cttatgagaa aaaagtactg taagtaaaaa
    10561 gacaactgct atcattaatg ttagcagttg tctttttatt tacaataata attgcagaaa
    10621 aagtaattca atcatttaag gtcatttatt ctatatataa atccaaatct gtttcaggat
    10681 attttaattt gacaagcgaa ataatcattt tcagttcttc catgttttct ggatattctg
    10741 ggtcaaatac cttgctatga cggccaatcc cgccattgtc gatcaatcgg taaattcgat
    10801 tccattcaat gaattggtca attgattgga aattgctgat tggatagccg ccacaatcca
    10861 aaatctctga tacacgaaat gctaggtatg cactatcgat cactttattt tttttcgtgt
    10921 ctatcttctc atttctttcg ataagaattt tttcaggacg atgtttcaag ctccactcta
    10981 atagtagaaa gaaagcctcc atccgatact cttcttcaaa taaccgaaag gctgaatcag
    11041 aacgacgaaa aagcagcgga atcagataat tttgtctata aatcagcatt ggtgcgcggt
    11101 ccaccataca ctctaaataa cggttcagat accctgtacc gacatagttt cctaatcgat
    11161 agccttcaac gccgaatctt gcgtattttt gcgctaattt tgaacaatca gttacgttca
    11221 cagctaactc ttctactttg cggtaaccgc ttaagatacg ttcaaaaggt tctaaagtga
    11281 tttttgcttc catattcttc cccgtccatc ttccacatac ttcatatcat ttcacatgat
    11341 acttatgctc tcatcatacc acaggttgaa ttagaaaaga aaataaatca cacaaaaaag
    11401 caagacaatc tgttagtgag attgtcttgc ttttcgaaaa agactgaata cttttcgtta
    11461 aaactggttg cttagtcttc taatgccaca ttatggaaca catgctgtac atcttctaga
    11521 tcttccagtg catcaatcat tttttcaaat gtttcttgat cttctggaga tagtgttacc
    11581 tcgttttgag ggatcatttg gatctcagcg attgaaaaat cagtgattcc tttttctttt
    11641 aatgcttctt gtatactatg gaaatccgct acttcaccgt aaacgatgat ttgtccttct
    11701 tcttccatta catcgcgaac atcgatgtct ttttccatta ggtattcaag gatctcgtca
    11761 gcgtcttctc ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga aaccgctccg
    11821 gaaacaccca tgttcccgcc atttttccca aatgctgccc gtacgtctgc tgctgttcga
    11881 ttcacattgt tcgtcaatgt atcaacaatc accattgagc cgcttggtcc aaatccttca
    11941 tagcgcaatt cagagtatgt ttcatctcct gcaccttttg ctttttcgat tgctcggtca
    12001 ataatatgtt ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg
    12061 tttgcatgcg gatcaggatc acctgatttt gctgccgcgt agatttcaat cccaaatttt
    12121 gcataaatcc ggctattatt agcatcttta gcagttttct ttgctacgat atttgcccat
    12181 ttacgtccca tattttcact tcgctttctg atttgatagt ttcataacgc aaaaacgtcc
    12241 tgttccatta tacttgtgaa tcacgattct gtgaagcttt ttttctttct tccttcattc
    12301 ttctcatttt tagtcaatta tctatactcg gattgggatt tgcccgaaat tctgttccgg
    12361 aatattgcct cccaattgat agatttgatc acttctaact aacagttccg cttcttgtgc
    12421 ctcttttttt ccgattcggg aataaataga aatctctttt ttttctttta agaatcttct
    12481 tccagcatcg gtaaatccaa gtagtcgaag ataattttta ttttgttgga caggaatctc
    12541 ttgctttttg atattcagca agacataaca agataatcgt tggatccgtg tccatgtata
    12601 tctttttgtt tttacctttg acacaaaatc ctcaaaacta tcagcagttg cagctgctgt
    12661 tttcatgcgc gattctattc cttcggtcat ctgatagata tctgtcagtt ccagcaaagt
    12721 agaagataac aaccgataac gcaaatacgg ccaataagat tcccaagtct gcagcgaaca
    12781 agttgaaaga tcttcttctg tctgaggcgg cacaagtcca gctattcttt ttccttcttc
    12841 taacgcatgg cgaatggctg ttgcactggc tatcctctga tctgtgatga ttttttcatg
    12901 atagcctgca cttttacgtc ggatcggatg taattccata gggtggacat acttggcatt
    12961 ttcctttgca taacttaatc ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc
    13021 gggatacaga ctgcgaaaaa cctcagtcat tttttgcgga taactatacg tttgatcaga
    13081 cagttcttga aatgcctctt ctatcctatc ttgattctct tggacaaagc gtccgaaact
    13141 gctgtaatca aacgaagtct cttcttctgt tccaaaacac aaactgtcac agtccagtgt
    13201 ttgcaagatt tttactgcac ctttagcaaa atagtcagct gactgaaccg cccattcgac
    13261 gggaagctcc actactaaat ccacaccatt ttgtaaagct tcattcgcgc gatgccattt
    13321 atcgatgata gccggttcgc ctctttgcaa aaaattcccg ctcattacag caatgacgac
    13381 ctctgctcct gttatttttc gtgccatttc cacatgatag cgatgtccat tgtgaaaagg
    13441 attgtactct acgataatcc cgcagctttt catgcgcatc ctccttcata gttatcgata
    13501 aaaaagaaat agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc gaactatata
    13561 aactattctc gcaaacacaa tacttctgcg acgaaaagct gcactgcaag agcaatgcct
    13621 atatcgttcg gatatataat ttatgttgaa gatttattct tttcttgtta actcgagcag
    13681 gcaaaaccgc tagtctttgt atgctacaaa gaaccagcgc tttgatttct ctgatgctgc
    13741 ttcgtctgta aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt ccagcattct
    13801 taaataactt tccaatggat aggtacgctc ttgatgtagt tcatcttcac gaataaatcg
    13861 accagaccct tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc
    13921 gcttcctgga taactatccc acaaaaaagc aaaattatcc ccttgataat gatagctata
    13981 ttctggaaaa acctcatcta cttgataggt ggagtgtacg tcaaataaaa atcggccatt
    14041 ttcttctaac gcttgataga cttcatcaaa cacttgctgt acctcttgtc tatttttcat
    14101 atagcacagc gaatcagaaa aacaggtaat tgcctgatat tgtccgattt ctgataaatc
    14161 gagcatattg ccttccacaa attggatcga tgcgtcttct tcatatgctc gctggctggc
    14221 aatcatcaac atctcttctg aaaggtctaa ccctgtcact ttatatccat cttttgcaaa
    14281 ggcaaccgct aatgctccag taccgcatgc cagttccaaa atattttttg tgtcttcttg
    14341 taaatgacgt ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca tcacttcatc
    14401 gtaaatgaaa gcaaatgttt cataagccat atgttaatcc aaccattcac taagatcgac
    14461 tagcggagca tctgaccata gtttttccag attatagaaa tttcgttctg gcgcatggaa
    14521 aacatgtacg atcacatctc caagatcgat caatacccat tttccgcctt cttttccttc
    14581 aatacgtttt acttcatatt tgttttcttc ttctttatca atgatttctt ctgtaattgc
    14641 gttgatttga cgttcgctat ttgctgaaca gatcatgaaa taatctgcca acaaggaaac
    14701 atttcgaacg tctaaagcga taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc
    14761 aatttttaat tgttctttac tatctatgat gatttcctcc taatatgagc agcttatttt
    14821 tttgctaccc agtaattata tgtttcgatt gtttttggat aaatctgttg ttcttgcgca
    14881 agcaaatggg ccaatgtatg ttttgtttca aatgctacag cttcgtctag atcagcccat
    14941 gcaatggctc gtgcatcttg taccccagga aaatctcgac ctggttcaat atagtctgct
    15001 acataaatga ttttgtcaag gagactcatc ttagcagccc cagtcgtatg cacccggatt
    15061 gcatgaagaa tctctgcatc ggtgattcct aattcccgct ctacaaacga agcgccaaca
    15121 agaccatgcc agattgcatt attataattc aataactccg gatcaaaacc gtctcttacg
    15181 attaccattt tgaactcctc atctggtcgt tcttttgcat aatcatgtgt caatgcagca
    15241 atactagctt tttcaggaga agcaccgtat tttttggcaa gagccacggc tgtttcttct
    15301 actccaagca catgcttgaa gcgttgttcg ctcattctca tctgtatttt ttgcatcagg
    15361 ttttctctag aataagcagt atattcatta ctatagtcca tattcataca acccttcatt
    15421 ttgaatataa tcaattactt tatctggaac aaggtagcgg atagaacagc cttctttgat
    15481 cttttgacgt attttcgtgg aactgatgtc gatctcaggt acatctaccc aaattacagg
    15541 atatggagta tccgttgtgt atcctggtcg acgtataccc acaaagttga ccatagaagt
    15601 cagctcatcg attttatacc attttggcaa atattctacc atatccccac cgataataaa
    15661 gtaataatct gtatccggat tgttttgcgt caattccttc atagtatcat aggtgtaact
    15721 tttcccacca cgagctaatt caatcgtttc aatctgcaaa aaaggattgt cttccaccgc
    15781 taattccaac atgttgagtc gatgttttgc atcaatcgtc tgtttctcat caacatgagg
    15841 tggctgataa gaaggcatca aaaatacgcg gtcaagacct aaattccggc cagcttgttc
    15901 agccatcact aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga tccctacttg
    15961 ctttttcttc tctaagaatt gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt
    16021 cttagcttgc gtattcatca tggtcactcc ttatattgct tttacttctt ttgatagcct
    16081 ttgatacttt tcttttgtgg aaggtttgta taatacaata acacgtccaa tgatttggac
    16141 gatttcgcaa tctatttctt tttctaatac ttgagctgct tcttctgcaa tctcatctgt
    16201 attttgaagt aacgatactt tgatcagttc tcttttttct aaagcttctt caatttgaac
    16261 gatcattgct gaattgatcc caccttttcc gatttgaaaa atcggctgaa gatgatgcgc
    16321 tttgctacgt aaaaaacgct tttgttttcc tcttaaactc ataattttcc ttcttcctta
    16381 aatcaatgct ttgcgtttca atacatctac accctttggc gcccagcccg caattactac
    16441 tggcgctgtt acagtgatcc agcctagacc ggcaaaaacg atgtctgttt tttctttcac
    16501 agaaaactca aaacgaacaa gctccggaaa tgcctcgact tcatcaggac gaggaggctg
    16561 taataatcca cctacatgtt tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg
    16621 aatatttaca tcatttgaca cataagcaat aaatgaagca cgattgccgc tgataaaatc
    16681 aaatcttgca agtccgccca agaaaagtgt ttgcccttca ttcagctgat aaactttagg
    16741 tttgatttct tttaacggtg caatgatccg caaatctttt ttacctaagt agtgcgccat
    16801 ttgatggcga tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat
    16861 ctcgatttta tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc
    16921 cgctgtattt ttgatgattt gattgataag cgtcgatttt ccgacatttg ttactccgac
    16981 gatatagaca tcccgtccat cgcggtattc ttcgattttt tctaatagtt ccgtcatctc
    17041 atgagatttc ttagcactag ttaataatac atctactgga cgaattcctg cttcgtaagc
    17101 acgttccttc atccattgtg ttaatttagt ttttttcaat gatttaggta atatatccac
    17161 tttgttccca actaaaagta ctggattgtc accgacaaaa cggtgtagtc ctgggattag
    17221 tgaaccattg aagtcaaaaa tatccaccac attgacgatc aatgcatctt tttgccctaa
    17281 ttcattcagt aagcgcaaaa agtcgtcatc cgtcaatgag acatcttggg tctcattata
    17341 atggcgtaat ctgaaacaac gctgacaata gacttccccg gaagctaaac ctttttccaa
    17401 agccgtttgc ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa cggcaccaca
    17461 gccgatacat tgtaattctt ctgtcattcg attccacctc gccagcccat atcagggtgt
    17521 tttgctaata gataggccat aattcttttt tcccaaaaac gattaaattg tgtcttccaa
    17581 ctatctgttg cgataatggg ttttacaagg atgctacgta tccctgcacg attggctcca
    17641 cgtatatcag tcataatctg atcgcctacc attactatct catcatctgc taaatccaat
    17701 tgtttctttg ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa
    17761 agatcaaatt tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga aacaacaaca
    17821 acagggattc ctgcattttt catttctaaa atccaagtaa gcaactcttc tgttccatct
    17881 ggattattcc atgcaatcaa cgtattgtct aaatcggtca atactgcttt gatgcctaga
    17941 ttctttaatt gggcaggtgt aatctgatag atggcttcta ccatccatgt tggtttgtaa
    18001 tttgaaaaca tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa gtactcctca
    18061 accccgtatt atactgcata tcagcaaaaa aaactacctc tatactcgtt cgtctttcag
    18121 aaaaagcatt tctttacaaa atgaccaaaa ataaagattg atcacagaaa aatttagaaa
    18181 caaaaaacaa ctaatattgg cggaacctct tgtatcgttt ttccagtaat ttatgctgat
    18241 ctaatcgaac aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt
    18301 tatatcgtgg caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta
    18361 actcgcgtag ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac
    18421 cgtctttcca caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt
    18481 ccagcatcca gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac
    18541 caatgatgat ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa
    18601 tagcctctcc ttcgccgcgc tcttctgctc ctacaccgca ataagcacct gctgtattga
    18661 tcaaggtaat caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc
    18721 gatagccttc aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc
    18781 ctttttgaat accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa
    18841 tcgcttcgtc atctgcaaat aaacgatcac catggcattc ctcgaaagaa tcgaagatct
    18901 cttcaaaaaa atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat
    18961 gtgctgtctt atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcgtatgg
    19021 attttctgtt tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag
    19081 aattcagcct tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt
    19141 cttccagcaa aaccgatcaa gctttggggt tctgctagaa taatatcgcc ttccattgca
    19201 aaactggcag ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct
    19261 tcattgctat gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt
    19321 ccttcttgca tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt
    19381 gttgctcgtt cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg
    19441 ataaaattag gatccatcac tccaataatg aagggaatcc cctgaatggt tgctttacca
    19501 gtgaggactg cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat
    19561 tcgggaaatg ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt
    19621 tcatcaataa tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga
    19681 gggcatactt tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac
    19741 tttgcccaca tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga
    19801 ttgatccgaa tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt
    19861 tctggtgtcc aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct
    19921 atgactgcag gatgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct
    19981 gtgataaatt cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca
    20041 tggacgatta tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa
    20101 actgcactgt ctactcgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc
    20161 cctggagaag gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg
    20221 tgtccttgga atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc
    20281 acctgttttt ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg
    20341 attcgagtat tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc
    20401 gttccggcat tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct
    20461 tctctttttg tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattgttg
    20521 cgttgtaaag aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag
    20581 atctgaactt ctatatggcg agcaggataa atgatttttt ccaaatacat atcatcattt
    20641 ccaaatgccg ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt
    20701 gattgaactt tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga
    20761 taaccaattc tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt
    20821 cctggaatga ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc
    20881 attgcatcga tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg
    20941 gaggcaaatc ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt
    21001 gtcacgattg cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc
    21061 gggccaatgc agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct
    21121 gaatatatcg ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg
    21181 atttcaccgc ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa
    21241 ggttaattcc gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc
    21301 ggctgaagct ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac
    21361 tttcttacgg aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg
    21421 ttcaagagaa agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc
    21481 tggcataaca ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt
    21541 tttcttagca acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat
    21601 tggataacga tgtggaatga tttcttttat ttcttgtatg ttcattgttt tccctctttc
    21661 ctgctttgct tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc
    21721 gttttctaca aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag
    21781 tttcatcgct tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt
    21841 gaaagctggt ttgtcgggcg ctggttgcag ataaacgatt cccacgattg gcgaaacaac
    21901 ctcgattcca tcaaatactg ccgattcttt ttctttcatt atttctggag cagttgttgc
    21961 ttgttcaggt atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat
    22021 attcatgcct cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa
    22081 atcgaattcc gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat
    22141 atccaaaatt attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct
    22201 aatgaattac tcattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc
    22261 aagtcaatct caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct
    22321 aatgccagca aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc
    22381 atcgatttcg tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt
    22441 gcttttgctt ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg
    22501 acttgttccg gctgtacacc agcttcttcc atcgcaagct tcattgcttt agatgcacca
    22561 gaaccatctg gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaacg
    22621 atttctccat agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga
    22681 acgcctgctc cttcacccat cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca
    22741 cgattaggat cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc
    22801 tcacaaatcg ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg
    22861 ttacggaatg cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta
    22921 caaatacctt ttgcacctac acgcaaagca atatttccag ctgccatgtt ggcaatcgcc
    22981 attggtacga acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg
    23041 ttttcgattg ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga
    23101 ttcatttttt ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca
    23161 tagatcgaaa acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg
    23221 aaatctttga cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc
    23281 ggtgcgatgc cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca
    23341 ataggtgaag tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt
    23401 tcttatccgt gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga
    23461 ttttgtgcta agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc
    23521 ggaatttgct tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt
    23581 gtgatgaatc ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc
    23641 acagattttg ttaatcctac tacgccagct ttactagctg cataatttgc ttgaccaata
    23701 tttccaatca acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc
    23761 atttttttca atacgtgctg cgtcatattg aaggttccaa tcaaattgat atccagacat
    23821 tttttgaaat cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg
    23881 tttactaaca catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct
    23941 ttctcataat cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt
    24001 tcttctatct tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct
    24061 tttgcaaaag ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca
    24121 ttttttcctt ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg
    24181 tctgcttatt ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc
    24241 ttaatacttt tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat
    24301 ggatactctc atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg
    24361 tttctttctt cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga
    24421 attgaatctg acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat
    24481 gaaaaggccc gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta
    24541 aaacagcttc gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat
    24601 aatttgcagg agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt
    24661 caatcggcgc attcatcact gcaaccattt tcccgcttcc agctggggca gcttctgtca
    24721 tataagcgcc tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag
    24781 ctactaatgc cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat
    24841 gctccttcaa taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt
    24901 gcgtctgatc caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa
    24961 tctcacttgc ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca
    25021 taccttgata ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca
    25081 atatatccag aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc
    25141 aggaaatgtc ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa
    25201 gtcactaaat cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca
    25261 tcttcaattt cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg
    25321 atgtcagtag tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct
    25381 tgaatttttt cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta
    25441 taatttaaca gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc
    25501 tgtaaatatt actttctgct gttttcctaa ggtgatcttt ccactagaaa ctgcttcatc
    25561 caataagata gggatgcttg cagcagatgt attcccatat ttatccatat tggtcaggaa
    25621 cttttcttgc ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg
    25681 atgcaaaagc agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac
    25741 gtctcgtacg gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc
    25801 tgaatcttct gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact
    25861 tctggtaccg tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa
    25921 gagaatccca cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat
    25981 tagcttagat agtgtttcac caccaatcac cagtccctta gttgagccgc agcggatcat
    26041 tttttcagcc atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc
    26101 gatagcatga gttgctccca atttcccttg tacttgtgct gctacagatg gtgtcgtgta
    26161 atcgggcgac attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg
    26221 ttttatcaat ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca
    26281 tcttcgttca cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt
    26341 ggaaagatca ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat
    26401 tccaaattgt tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt
    26461 cagtgcatcc agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac
    26521 catttcacga tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc
    26581 caactttaca acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac
    26641 taacttgtta atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc
    26701 tgaagttgtt tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga
    26761 cagatctttg aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa
    26821 gacactaaca agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact
    26881 aattagtttg attatcaaat cttttgatgt tcaaactata tcttaacttt tttcaaaatg
    26941 caaccttaaa agatcatttt tttactatat gagctttttt taagatttta ctcatttaaa
    27001 acaactgata aatcagcatt tataatttct tttattagaa aaatcatcta tctcacatat
    27061 tttgattatc aaagtatttt ctgtgaacag aactatagaa ttccctaaaa cttgtccatt
    27121 atttcaaaat ttgttatagt ctttttattc aaagcgtaaa aggagatcct atgttaaaag
    27181 aaaaaatgaa aactctcccc atgctgccgg gtgtctatct tatgaaagac cgagatggta
    27241 aggtcatcta tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc tattttcatc
    27301 agaataaaca gcattcaaaa aaagtattac gtatgattca tcatataaca gattttgatt
    27361 tcgtcgtagt agacacggaa ttagatgctt tattgctcga gtgtcaattg atccaacatt
    27421 accgtccttt ttataaccga caaatgaatt atttcagtaa ttataattat gtacatatta
    27481 ccaataaggg atttgtatta acagatactc ctacggctcg aacatacggg ccttttcgtc
    27541 tctataaaaa aatgccgtct attttgcgta taatggaaga aacttatcaa atgccttggt
    27601 tgtctgaaat tagtttgtta gctttgcgag tccagcttcc tgacctacag gaaatgactt
    27661 ttgaacagaa aaaaaaggaa ctccaagggc tgtttcaagg ccgaaataaa aagctactga
    27721 cttacttgaa aaaaagacaa caacatttca tctatcagtt gaatttcgaa aaagccggta
    27781 tgcttcaaaa agatatcgaa ttagttactt attttattag acggatccag gaacaaaagc
    27841 agtttttaag aacaccatcg ctcaccttca gcatgccatt agcagcagat gagagtcaga
    27901 aaaagcacta cctcgtttgt tatggtcaac ttgcagaaac aatgattacc tctggtgacg
    27961 tttctcctga cttctattat gaaaagaaag aagctcattt atctttgaaa cgtcaattgt
    28021 caaaagaaga aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa ttagaaaaag
    28081 aacagtttga aattgaactg ttaaacaaaa aagaagctga aaagcaacta aattagttgc
    28141 ttctcggctt cttttaggca tcttcaactt caaatgtcaa ttatcctaaa atttattgga
    28201 ttaagtcata gatttccatc gctacgatat cgatattatc aaattcgtat tgctgatgag
    28261 tatctgtttc agttaattca aatgtatcgg tcgctttatc ataagttact gtacacttct
    28321 ctacgccttc tttttcaaaa cggcgaactt gcagttcatt atctgctgac tcgaccatag
    28381 cctccagtcg ttttatgatt gcgactaatt gtgaatgtgt catcggaggt cctccttttc
    28441 ttatttctgt tatattgtat caaaagcata gcctacttgc attgattttc tctaaaaaaa
    28501 ctcgtttttt atgatttttt ttctggtatt tttccttcag gccaccaaag tttgatcaaa
    28561 gcttgggttc cgtcattctc aaaaccttta tctgccaact cttcatatag tttcaatgct
    28621 tgggtagttg ctggcaatac aagatccatt ttttctgctt cttctaatgc aatctttaaa
    28681 tctttgataa aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa aatacgagga
    28741 gaataattgc ttaaagacca gttagctgca cttccacctg aaagcgtctc aatcactttc
    28801 tttaaatcaa gtccgttttt ttgtgcatag acaagcattt ccgtcattcc tgtcatcgta
    28861 cctgcaatca tgatctgatt agccattttt gtatgctgac ctttcccagc agaaccatgc
    28921 aatgtaaatg tcgtaccaaa ttctttgaat aaaggcagta cttgatcata aacggcttct
    28981 tctccaccga ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc accggatacc
    29041 ggtgcatcta acgcatctgc gcctttttct tttgctgttt ttgcaatttt ttctgccagg
    29101 cttggtgtac ttgtagtcaa atcaactaaa atatgtcctg agatatctgc agaaaaaatc
    29161 cccgactgtc catagtagat ttcttctaca tctttggggt aacctaccat tgtaaagaga
    29221 atattacttt gttctgctac cgcttttggt gtatctgccc agatagctcc ttctgttact
    29281 aaaggatcag cttttgcttt tgtccgatta tagacagcta cttcgtgtcc tgcttctagt
    29341 agatggcgtg cgacagcact tcccatgacg cctgttccga taaaaccaag tttcattttt
    29401 tcgacctcct aaaaatcaaa aatccttgct tctatgctag tatacgctag aaacaaggat
    29461 tttttcataa aaaatgtttg aattttatct tttaaatgaa tttaatttta ttttgattcg
    29521 ttcaatcgag tcttcttctt cttttcttcg catctcttct tcatgataaa caaccatgtc
    29581 ttgataaaat tcgatcatag cttcgccgaa atgtgtagat gaaatatcat aaagtttagc
    29641 ttctaaaact tttggatctt taggaacttc actggaaata taatcaataa gtgctggtgc
    29701 aaagtcttcg tcggtttcaa aagttttccc taatgaagga tgatcgaaca atcggttcaa
    29761 ataagcattt ccttccacga cacaaggtgt tccggctgcc atcgcttctg tataagtaag
    29821 cccttgtgtt tctgaagtag aagcactgac aaaataatct gccgctttat aataaagtgc
    29881 tacatcattg ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact gcagttctga
    29941 gatcaattcc tctaactctt ctacataagg tccttttcct acgataacta atctggcgtt
    30001 aggataagcg gagatgacct gaggaaaccc attaatcaag gcttggatat ttttctcata
    30061 agaaatcctg cttaaagaaa gaatcatcaa atggttttcc tgaagaccta gtgactccct
    30121 taatttgctg atatcctgct gagtgatatc agaacgtttg aatttttcaa tgtcgatacc
    30181 agtaggaatc acgcgaagcg gtgctattac accatagctg cgtaatgtat caataacacg
    30241 ttcacttgga caaactacac ctgtcgtatg atttgcaaat aatctagaca agtattttac
    30301 atgggattgc ctgatcactt ttcctttacc gatataatga aggtagtctt catacatcgt
    30361 atggtaagta tggatcagag gtattttcaa cttcttagct accatcttcc caagtatccc
    30421 tgctccgaat tctgtatggg tatgaataag atctagttct agttctttgg caaccaaata
    30481 agcataccac atgcctctaa caacaactct tcgatccttg aaagaaataa atggtacact
    30541 tggcattcga atcacatctt tttccaactt atccgcatta ggatcggtcg ttgtaaaaat
    30601 atagacttca tgcccatgtc tttctaattc ttgttttaat gttttgatcg atgtagcaac
    30661 tccgctgact tgcggaaagt aggtatcggt aaaaaagccg attttcatgt ttttcgcctc
    30721 caaactagtc cttgacctga tttacttttt ttaatttgct agagactttt tcattcagta
    30781 cactttcgta tacattttta agttcttcac cgattttatt gatacttcgc tcttttgctg
    30841 tttgataacc ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt tggatcagtc
    30901 ggataaattc ttcgtttgtc ttccccatat agcagttctt gctatcttct aaccagccat
    30961 tatacactgg aatatctcga acaagtacgt tttgtttgct tgctaaagct tctaaaacaa
    31021 cgatcccttc tgtctcttca taagaaggga aaaagaatag atctgcacct gaatacgcac
    31081 cttctatgat ctctccacga atgtaaccag gaaacaggac attttcagga tgatcttctt
    31141 ttacaatttt ccggatgttc cgaggaatcg agtacatggg aacatgacca aaccaaataa
    31201 atgtgtattc gggcatcttc ttagcaattt ctacgaaatc aatgattcct ttacgttcga
    31261 aaaacaaacc gacacaaatg attacttttt gatccggaga cagtttgaaa tattctctga
    31321 acttttgttc tttcattgga tcagggtgat atttctctaa gtcgatccca ttggaaatag
    31381 cttgaatcgg cacacggata ccatagcttt ccaacaactg ttttgaataa ggtgttggcg
    31441 tgatcagata gtccgctttt tggtacaatc ctaccagata tttttttacg ataggagaaa
    31501 gctggttcga tctgataaag gaatttcgga aatcttcctc tgtagaatgc gcatgataga
    31561 caactttttt tccttctctt ctggctttat tgaccatatt atgactattg attccgtatg
    31621 tattgatgtg caggatgtca taatcttctt tttcatctag tgtataagag ataccgactt
    31681 ccgtcagagc acgtttttga tgatcaagcg cacgcccgat tccagatttt gcaagcatct
    31741 tttcactttc aaaatataat aataccttca ccttacgtcc cccttcactt atatagtata
    31801 acatagccta caaactgtca ctcaaactta agccctattt ttgtctattc ttactgacaa
    31861 aaacgttttc ttaaaaaaga acaaaaaaac tggcgtagct cttttacaag ccacaccagc
    31921 ttttgatacg ttgtttattt tacatattca tgaacaagtt caacgacttc ttccatcata
    31981 tcgcattctt tcaatgcacg atcagcaagt tcagccattt tcgctgtgtc caagcgtttc
    32041 attaaactac gcgttttaag aatagatgtc gcactcattg agaactcatc tagacccata
    32101 cctactagca atggaacagc tgtttgatct ccagccattt ctccacacat acctgcccat
    32161 tttccttcag cgtgtgctgc atcaattaca tttttgatca aacgtaaaat tgatgggtta
    32221 tatggttggt acaagtaaga aacgcgttcg ttcatacggt ctgctgccat tgtgtattga
    32281 atcaaatcgt tcgtacctac agagaagaaa tctacttctt ttgcaaactt gtctgctaag
    32341 actgctgctg ctggaatctc gatcatgatg ccgacttgga tcgtatcaga tacttccttg
    32401 ccttcactta tcaatttttg tttttcttct tcaaagatcg cttttgctgc gcggaattct
    32461 ttgagtgtag ctaccatagg gaacatgata cgtaaattac catgaacaga tgcacgaagc
    32521 aatgcacgca tttgtgtacg gaacatttcg tcgccttgtt cagataaact gatacgcaat
    32581 gcacgatatc ctaagaatgg attcatttca tgaggtaatt gcagatatgg tagttcttta
    32641 tctccaccga tgtccattgt acgaacaact acaggttttc cttccatacc ttctaataca
    32701 gctttgtaag cttcatattg gtcatcttct gttgggaagt ctggagaatc catatacagg
    32761 aattcagtac ggtacagacc aactgcttct ccaccattat tatggacacc aactagatct
    32821 tttggtgttc cgatgtttgc agctaattca aaatgcttgc cgtcagctgt gacagtttct
    32881 gcatttttta atttttccca ttctgctttt tgtgctgcgt aatctgcacc tgctttttca
    32941 aattcagctt tttgttcatc tgtaggatcg atgattacat cgccttcgat cccgttgaca
    33001 gccaaaatat cgccttcttt gactttagca gtaatttctt ttgtcccaac gattgctgga
    33061 atttctaaag aacgtgccat gatagcagaa tgtgaagtac gtccaccaat gtctgttaca
    33121 aacgctttta caaaattgcg atctaattgt gctgtatcac ttggagtcaa gtcatgtgca
    33181 acaacgacca cttcttcgtt gatcattgat ggatttggta aagtaacgcc taataagtgc
    33241 gctaaaatac gtttagcaac gtcacgaata tctgcagcac gctcttgcat ataagcatta
    33301 tcttccattg cttcgaacat accgatatac atatccgtta cttcttttag cgctgattca
    33361 gcatttacgc tgttgtcttt aatattttgc ttgatttgac ctaccatttc tggatctgaa
    33421 agaaccatca aatgggcatc aaatacttgc gcttctgctt cacctaagct ttgcgctgct
    33481 ttttcacgaa tttgctgcaa ttcttcagta gatttagcta atgcagcatc caaacgagct
    33541 tcttcagccg cagtatcttc tactgttgcc ttactgaatg ttaaatccgg ttgaactagc
    33601 aggtaagctt ttgcaacggc tacaccgtca ctagcggcaa tcccttttag catttcaacc
    33661 attattcaga taagccttct ttcttcattg tttcaacgat tgctgccatt gcatcagctt
    33721 cgtcagcacc ttctgcagta atagtaacat cagaaccttg acctacacct aaagacataa
    33781 cgcccatgat tgatttcaag tttactgatt tacctttata ctctaagttt acatcagagt
    33841 taaatttgct tgctgtttgt actaatagtg tagctggacg agcgtggatc ccagtttctg
    33901 ctactacgtg aaattctttc ttttccatat taatcggtct cctttaagtg agtgttgttt
    33961 ttatgtgagg gttttctttt ttagtaattc agagaatcct aacaaatgcc accctttcat
    34021 ctgtaaagat taacattttt tttcgttaga tacaacagtt ttcattcaat ttcagataaa
    34081 agtattcaaa ttattccgga ttctttttca tccttttctt cacattttat aacaaacgtg
    34141 tttatttcct ttttcttgtc gctttctctt ctcctgacgt ttatcattct ttcctttgac
    34201 cctctttgtt ttcatatgaa acgaattact tgcttccctt tttattttta gtataataag
    34261 agtcaaaggt caggaatggt caatagagat ttcacctttt ctacctcata aatctcttaa
    34321 aaaactaggg gttattcttt tacctcatga aaaacgagat gtaaaatatg gatgtaccca
    34381 tttttgtgaa aggacgtgaa tgattcatgc tttgtcaaaa ctgcggcaag aatgaggcaa
    34441 cgattcactt gtatgccact gtcaatggtc agcggacaca actcgattac tgtcagagct
    34501 gttatcaaaa aatcaaaaat caacaaaatg gaggtctgac taatatggcc caaaatgatc
    34561 catttggatt tggcagtttg gacgatcttt tccgttccat gtctcgccaa atgcaacagc
    34621 aaggaaatta tgagcaaaca ccaccaacac aatttggcgg aaacaacaac ttcaatggtg
    34681 gtcagccacc tcaaggcaat acagaaggtt tattgggaga atacggaatc aatatcacag
    34741 aacaagcacg acaaggtgat atcgatcctg tgatcggacg cgatgacgaa atcaaacgag
    34801 tcattgaaat tttaaatcgc cgaaccaaga acaatcctgt cttgattgga gaaccaggtg
    34861 ttggtaaaac agcagttgtt gaaggtttag cacaaaaaat cgtagatggt gatgtaccgc
    34921 aaaaattaat ggacaaagaa gtcattcgtt tagatgtcgt atctttagtc caaggtactg
    34981 gtatacgagg acaatttgaa gaacgtatgc aaaaattgat tgaagagatc cgtcaagcag
    35041 aaaatgtgat cctattcata gatgaagttc acgaaatcgt cggtgctggt tctgctggtg
    35101 atggaaatat ggatgctggt aatattttaa aaccagcttt agctcgagga gaattgcaaa
    35161 tggttggtgc tactacctta aatgagtacc gcatcatcga aaaagacgct gctttagaac
    35221 gtcgtatgca acctgtacgg gtagatgaac caactgtcga agaaacgatc agtattttga
    35281 aaggtttgca aaaacgttat gaagactatc atcatgtaaa atatactgat gaggccatcg
    35341 aagcagccgc aactttatcc aatcgttacg tccaagatcg cttcttgccg gataaagcca
    35401 tcgatttgct agatgaaaca ggttctaaga aaaacttgac gatccaaatc gttgatccta
    35461 aaacaatcga gaaaaaactg caagaagcag aagaacaaaa agttttagct tctcgagaag
    35521 aagatttcga aaaagcagct tattatcgcg atcagatcaa taaacttcaa aaaatgaaag
    35581 aacgtcagct cactgaagag gaaacacctg tgattaccga aaaagatatg gaaaaaatcg
    35641 ttgaacaacg gacaggtatt ccagtgggtg aattaaaaga aaaagaacag acacaactga
    35701 aaaacttagc agatgacttg aaagctcatg tcatcggaca agataacgct gtagatcgag
    35761 tagcgaaagc gattcgccga aaccgtgttg gattaaacaa acagaatcgt ccaatcggct
    35821 ccttcttatt tgttggacca actggtgtag gtaaaacaga gcttgcgaaa caacttgcct
    35881 acgaactatt cggttcacaa gattcaatga ttcgtttcga tatgtctgaa tatatggaaa
    35941 aacatagtgt ctctaaattg attggttctc ctccaggtta tgtcggatac gaagaagctg
    36001 gtcaattaac agaaaaagta cggagaaatc cttacagtct tgtcttgtta gatgaagtag
    36061 aaaaagctca tccagatgtt ttgcatatgt tcttgcaaat cttagatgat ggtcgattga
    36121 cagatgcaca aggaagaacg gtcagcttta aagatacgat catcattatg acaagtaatg
    36181 ccggaactgg taaagtagaa gcaaacgttg gttttggtgc ggcacgcgaa ggtgttactc
    36241 gttctgtatt gaaccaatta aacaactact ttacaccaga atttctgaac cgttttgacg
    36301 ggatcatcga attcagtgca ttatcaaaag aaaacttgat gacaatcgtc acactaatgc
    36361 tagacgatgt caaccaaatg ctggcagcac agcaacttca catcgaagtt cctacgaatg
    36421 tcaaagaaaa attagttgac ttaggctatg atccatctat gggtgctcgt cctcttcgac
    36481 gcacgatcca agaacaaatc gaagatggta tcgctgaatt ttatcttgat cacccttcta
    36541 tccatgagtt gaaagcaaaa ctggataaag atggaaaaat catcgttact tctaaaccag
    36601 aacgtttggc taaagaatca gccgaagaaa cggctgaata aatccagttt tatagcgtga
    36661 gacggtattt ctttgagaaa tgccgtctca ttcttttttc agaaaagtca aaatactgtt
    36721 cattttataa aaaagaaatt ataataagag aaacaagcaa ttaaaaatac ataaaagagg
    36781 tgagcgccat gaaattggta aatgtaacaa acagccattc ccgtcttgta ttaaaccagc
    36841 ttgaaaacac tgatgcccat ctagtcaagg tctacacagc cggaaatacc actattgtct
    36901 acactgaagc ccctgaacat aatgaaattc tgctgatcaa tgacaaacgt aaaatccagc
    36961 cgaaagaaat cgaagatgct aaaaattatt tcttagataa aatcaaagac gccgtttacc
    37021 acgaggatga aatcaaagta atcgaattgg caggccttgt tgaaatctcg attccaaaac
    37081 atgaattagg cgctacatta gccgtgtaaa caaattttga acataataat taaagagcgt
    37141 gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca
    37201 gaagcaactc cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga
    37261 attccagctt atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt
    37321 tttgattttt ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct
    37381 ttatttttaa ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg
    37441 tttttgtccc agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc
    37501 tgtttttttc tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta
    37561 atcatatgta cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat
    37621 atagtaaaaa gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg
    37681 tttttgtttg gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca
    37741 tcaaactttt taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt
    37801 tctcgaataa gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac
    37861 tcatcttctc atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag
    37921 acatgattac ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta
    37981 gctgaccaac agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg
    38041 ctccttcttg aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt
    38101 ttgcagtcac tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt
    38161 ttagttttcc ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga
    38221 tgatatcgcc tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga
    38281 cattgcttaa tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga
    38341 atgttccaga acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg
    38401 cttggatctt gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat
    38461 cttgtgtttt gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct
    38521 gtacaccgaa gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa
    38581 ttttttcttg attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt
    38641 gttcatataa ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca
    38701 attctactaa tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt
    38761 agatatcata aagaccttct agccctttac ccataccgat tggccaattc atcggataag
    38821 attcgatatc taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac
    38881 gatctagttt gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata
    38941 atttctttgt ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca
    39001 cggccatcaa agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga
    39061 tatttactcg tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac
    39121 gttgtttctc gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta
    39181 cggtcccggc ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt
    39241 tcccagcatc cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta
    39301 attttggatt attcataacc taactccatt tcttccattc aattatttat attttctcta
    39361 ggcacagatt gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc
    39421 gtgtagcagt ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc
    39481 ccacccaagc caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca
    39541 ggatagcttt ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca
    39601 gcgaatgaaa aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag
    39661 aaaagacgtg ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat
    39721 cttcttggct cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc
    39781 gcttctgttt cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc
    39841 atttcttcag aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc
    39901 ggaatcatgc ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc
    39961 agatcactgt caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat
    40021 tcgtgttcat cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct
    40081 ccgacgattt cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca
    40141 tctaataaaa tagccatctg attctgcgtc tttttcattt catataacag atcatcaata
    40201 aagaccgttt caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca
    40261 aagccgaatt tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta
    40321 tctttgtctt cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca
    40381 ttttcttcca cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact
    40441 tctcgggcaa ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta
    40501 ttcaatacgc cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct
    40561 tcatcatcaa aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc
    40621 cagacgaagg gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg
    40681 acttcttctg atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat
    40741 acataggtca aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca
    40801 cctaataccg gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg
    40861 ttgaccaaag tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt
    40921 tttaaaagct tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta
    40981 ttgatcgaaa caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg
    41041 atcaataata aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca
    41101 ttctctcctt ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca
    41161 cgttaaaatg gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac
    41221 ctacgaggtc gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt
    41281 gattatatag gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc
    41341 aagcaatccg gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat
    41401 tttcagtgat ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat
    41461 tagcacaacc acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa
    41521 tgcaggtgct agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat
    41581 gaccccaaat aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt
    41641 accaacgaat aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag
    41701 ttgccccaat aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt
    41761 tggcactagt ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat
    41821 tactacggtt gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact
    41881 aaggaattgc tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat
    41941 atctacttcc ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt
    42001 gatgaaatca ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac
    42061 actaagtaaa atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca
    42121 tattcttttg atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag
    42181 aaagcctgca atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa
    42241 aataaagttt atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga
    42301 ccaaaacatt agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct
    42361 tactagtata tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt
    42421 gccatacttt ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc
    42481 atgaaagttg tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc
    42541 aggcatcaag tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat
    42601 gaagcccatt gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta
    42661 cgtcttttgc ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa
    42721 tacagacacc aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat
    42781 caaggctata atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa
    42841 ctaggatata cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg
    42901 aaaaagactt tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa
    42961 atctctcact ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg
    43021 ttataccaaa tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca
    43081 accagaatct tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt
    43141 ttgctgtgca tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc
    43201 ttgatatcca agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa
    43261 gctagattac aataatagga aacccccttt tctctgatca ttgctattac attgaaccag
    43321 tattttttat gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat
    43381 tcagtgacta atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc
    43441 atggtatctc gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca
    43501 aactctcctt cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg
    43561 tgtttgggtg ttcattacac cgacatcata attattatat caataaaaat gagctttgcc
    43621 tagaaaggat tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc
    43681 ttaggaaaaa cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg
    43741 gtaaattctt gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc
    43801 agatgactaa aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc
    43861 tgcttttcaa atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt
    43921 tctggctttg tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc
    43981 attggaaatg tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac
    44041 gctccagaat gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt
    44101 tttgttttta caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg
    44161 atcggacaag tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc
    44221 attgcttggt tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca
    44281 aagattttcc tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact
    44341 actcgcatca cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc
    44401 gcgcctgtcg tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc
    44461 atttccccat caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt
    44521 gagtaataac caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc
    44581 tcttcaattg taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact
    44641 ctggtttgct gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc
    44701 caaggcaggt ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt
    44761 gtttctttat ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat
    44821 gaatcgctgg atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag
    44881 atttcttttt ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac
    44941 taaataatca tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg
    45001 gatcacttct tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc
    45061 tttttggctt tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg
    45121 aacttggtct tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc
    45181 tttttgctgc atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt
    45241 gcgcacataa ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc
    45301 tacgatttta tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc
    45361 tttttccgat cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc
    45421 tccgctactc ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa
    45481 tcgatagcgt actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac
    45541 gcgatagaaa gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct
    45601 cgaacatata ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga
    45661 ataaaccaca gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg
    45721 acaaacggtt cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt
    45781 caaacggttg atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat
    45841 cacgaaagca actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa
    45901 agatcgcgta gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca
    45961 cttagcttat caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa
    46021 attcaaacca gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc
    46081 ttggcattat agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat
    46141 tgaagctggt ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt
    46201 tcaagaacct cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga
    46261 tgcacccatc tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat
    46321 cggtgttaaa actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca
    46381 aaaaaacgca atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca
    46441 aaatgtccag gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg
    46501 aaaagaagcc attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt
    46561 ctttcaactc aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga
    46621 tgtccagcct aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag
    46681 tgtagcaaaa caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga
    46741 tgcaaaaaaa aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc
    46801 agagcaagtt ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga
    46861 tccaccacgt acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa
    46921 aaaacttgtt tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag
    46981 taaaaagttc aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg
    47041 tgaagttgtc gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc
    47101 tgccctgtat caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata
    47161 ctgtacttgt gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga
    47221 catagttttt gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata
    47281 aaatgcattt gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg
    47341 atcctgcgga agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc
    47401 tatttttgtg atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg
    47461 tttggtaatg tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat
    47521 atttaatgtt cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa
    47581 aggctcaaag ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt
    47641 agcttcgata ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc
    47701 tgctgatgtt cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc
    47761 tggaatgcta aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat
    47821 ctttttcatg tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta
    47881 acacaaacat tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat
    47941 gcagattata acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat
    48001 atgatctttt tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa
    48061 ttatcaattt ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt
    48121 aaactaatat tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg
    48181 gtttgtgtta taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc
    48241 ggaaaatatt actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg
    48301 ccgatgtgga ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac
    48361 aacaacaaaa aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat
    48421 tttatatggt ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt
    48481 acacttttag ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg
    48541 gcaatgtggc tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat
    48601 tcaatttatt ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa
    48661 aagtgccacc taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact
    48721 atttttttct agtacccact tgttgttcca tactcattat tctggtcata accataacca
    48781 ttttgttcct gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac
    48841 tgacttgtat cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg
    48901 ttttctgaat aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc
    48961 gaatcgccat cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta
    49021 ggtaatgtat cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga
    49081 agatcatttt ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc
    49141 aactggtctt gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg
    49201 tcatcccaat ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa
    49261 ttgcttacgc catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga
    49321 cgacgttgtc gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa
    49381 cctttttctc cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag
    49441 tcaattttat tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc
    49501 aaattgatcg ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg
    49561 gccatttcga caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct
    49621 acgatattcg tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga
    49681 ttaactgtta ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg
    49741 gtatctaaac ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca
    49801 gaacgtttgg aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct
    49861 tctcccataa atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct
    49921 aacaaggtta atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct
    49981 aaaatattgt ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact
    50041 ttaaaaaaca tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta
    50101 tcataccata ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat
    50161 ctttttttat gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg
    50221 cagtaaaatg atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga
    50281 ttatcattcg atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt
    50341 ctattcgttt tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc
    50401 attaccatca tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt
    50461 aaagtaatag aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt
    50521 tattcaacat ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc
    50581 ttgcttgata attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat
    50641 ctaaaaactt cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca
    50701 acaaattatt ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc
    50761 gtgaattcta cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag
    50821 aaatgaacaa atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg
    50881 ttgaaccatt taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa
    50941 gtaaccttgc aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta
    51001 ttttccaaat tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa
    51061 acgtttgttt acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg
    51121 gacattttta tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt
    51181 ctttccttcc atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt
    51241 ttccattaca agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc
    51301 ttctttaaaa gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt
    51361 aggttcgcca ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc
    51421 gccaccattg ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa
    51481 taattcttct attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt
    51541 cgtcacataa gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata
    51601 aggttccaat gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt
    51661 cctcctcaat gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt
    51721 cttcaattgt tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc
    51781 agtttcttta aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg
    51841 agtgatgatt ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc
    51901 ccccatgtcc aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag
    51961 tgcatgtgta aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc
    52021 aaacaataca gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata
    52081 ttggcgtgcg agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag
    52141 atagactagt atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg
    52201 cttcatgagc caacctacct tattaaaaat tcttatttat catactctat atttcaataa
    52261 ctttcatctt tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg
    52321 atcgtcacat gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc
    52381 ttttcaatct cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc
    52441 ttttcgctat actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca
    52501 atttcttgtt ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca
    52561 gcttttttga tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata
    52621 actggatcga tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt
    52681 accgaagaca gtgtatcact taacaaatgc aaataactag attttatatt caaactttca
    52741 tctgcttctt gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct
    52801 actgttagca tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca
    52861 acggttaaaa acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt
    52921 tctaaacgct ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt
    52981 gccccgtaag aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca
    53041 agagacaaac tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc
    53101 aaaaccacta ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct
    53161 tcctccattt tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc
    53221 accaatcatt cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt
    53281 gcaagtgatg acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt
    53341 tcctttatac gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt
    53401 gtctatcttt tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa
    53461 gtcttaaaca acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca
    53521 atccaaaacg ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc
    53581 attatcttca catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg
    53641 atggggaatt ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat
    53701 acaggcacga cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa
    53761 tctttaaaaa acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca
    53821 ctttggataa aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg
    53881 atcactttgc tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa
    53941 gctacttttc ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt
    54001 gcaacaatct tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca
    54061 ggaatcttta tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa
    54121 ataaagacaa ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt
    54181 gtcacagccc cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc
    54241 aaagttcttc ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca
    54301 gaaagaatca tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact
    54361 atagcgcctg gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag
    54421 atcagtttgg gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa
    54481 tgggtccaat ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa
    54541 catattttac tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta
    54601 ggaatcatcc ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg
    54661 aaatggttag gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt
    54721 ggaactggcc atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc
    54781 tatggaatca atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat
    54841 caactaaagg aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag
    54901 gaaacaactg ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc
    54961 atcaatgttg gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac
    55021 cagcaacttt cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat
    55081 cctctttgga aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat
    55141 tttcaaaaga aattaatggc tattttttta accaatctga aaagctactt tgccacccat
    55201 gagcttcaaa ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt
    55261 tcaaaagaaa aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt
    55321 taaagtgata ggttggctcc tccaggttgg gattataatc aacaagtaaa tcatatcctt
    55381 taaagaacca ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct
    55441 ttacaggatg ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg
    55501 ttttgccgta gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg
    55561 agtcaaacca cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc
    55621 tcttttcttt ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa
    55681 taaccagtag attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg
    55741 tttgatctac tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt
    55801 cagctaatgt tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt
    55861 tatctgacaa ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta
    55921 ttcccatgtc tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg
    55981 catgatactc attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca
    56041 gagtaactaa gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat
    56101 ttcttatgcc taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag
    56161 tggctatatt catatattca ttgacccgat cttcattact atcaatagcc agtacttctt
    56221 gaccagcttc gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg
    56281 caaaattttg tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc
    56341 tttacaaaaa caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt
    56401 ttctcagctt ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat
    56461 gcatagatag cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca
    56521 tgtgttttta ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt
    56581 ccttcagaaa tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca
    56641 ggctcatttc gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact
    56701 cgctcgccac gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc
    56761 aacaaatacc ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa
    56821 ctggttacga taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg
    56881 ataccatcca tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc
    56941 gccttctcat agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga
    57001 atagataaat aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact
    57061 tttatcatcc ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct
    57121 tgacctttaa aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata
    57181 ttgttcaatc cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca
    57241 ataatacgta acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct
    57301 ttagcatgtc ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca
    57361 atggaactag atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt
    57421 tgcaattcct tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact
    57481 ggacgtagat gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct
    57541 accattttca actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc
    57601 aatgccgaca tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca
    57661 cgggctaatc gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat
    57721 tggggacggc tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc
    57781 ttagtgattt ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga
    57841 agaagcaaca atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt
    57901 ttcccgtatt ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct
    57961 aaggaaatgg ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat
    58021 tctccccacc aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata
    58081 ataaggaaag ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc
    58141 atattcgtat cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag
    58201 ggttttcatc ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct
    58261 cttcttcaaa caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca
    58321 aaggtactaa gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt
    58381 ttggcaaaag cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga
    58441 tatcgtccca ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt
    58501 tccgctcatt attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc
    58561 gccaaaaagc atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa
    58621 ataaaacggc aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa
    58681 tgaccaaact tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca
    58741 tagctagata tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta
    58801 cgatttgcca aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag
    58861 aactattcat cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt
    58921 aactgttggc tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag
    58981 agaaaaagat ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact
    59041 acgcttgttc tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta
    59101 cttcatcatt tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt
    59161 tgccagaaaa cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat
    59221 ctggtagttc gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt
    59281 cgttatcgat gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa
    59341 caaatgcttg ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac
    59401 cgcgggcaat tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta
    59461 attctttttc taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt
    59521 gtaacgtctc cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc
    59581 aaaaaagatt accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct
    59641 agagcctgtt tttctaagaa ttatttacat atttttccac taattccata tgttcttcat
    59701 aagttttagc gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat
    59761 tttctgctgg ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag
    59821 gtccatagcc ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg
    59881 tcactaattc tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca
    59941 tatttgcttt gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta
    60001 cgccttcttt ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct
    60061 gcttgatagc tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca
    60121 ctagatcctt aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt
    60181 ccaaacggta ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt
    60241 ggtgcaactc gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt
    60301 taccagttac cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg
    60361 caattttccc atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc
    60421 caatctcgtc aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta
    60481 aatttttaaa ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct
    60541 ccaaaatctc accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt
    60601 ggtcatctga tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc
    60661 caagaaatcc gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa
    60721 tcttatcttc ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct
    60781 tactggcttc ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc
    60841 cttactatca agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat
    60901 tttatttaaa tattctatca aaaatgtaag tagtaaatcc attactataa gattaaaata
    60961 aatgttatca agatacaagc tattttagct ttattctttc tgtaaaaaag aaaggagact
    61021 aaataaacta ttatgaaaaa aggaaaacat gctaaaaaaa gaaaatattc atttttaatg
    61081 atcgttgtcg tgttgtttag ccttagtgcg ttctctacag gaactattct tggatggcaa
    61141 ttttacaata atgaacactc tcagatagtt gcccatgctc aatcagctca aaaaaagttg
    61201 atactgacta ccaatgaaca acagctcgtt gaaaaaatcg atcaactatt aagaaaaaat
    61261 gattatattg gctcaattta cgtaaagaaa aataatcgca tcatcatgga aaaaggctat
    61321 ggctatgcga ataaactcac gcaacaacca aatgatcctt ctctttatta tcagattggt
    61381 tcggttcaaa aagcattgac tactcttttg attttaaaac aagtcgagcg tgggcacctt
    61441 tctttagata ctaagctggc tcaattctat ccacaaattg ctggcagcca gcaaattact
    61501 atccgtgact tgctgtatat gcgctcaggc ttgcaccgaa ctgcttctcc aaccataccg
    61561 atgactgatg aagaagtcgt tcaatttgcg attcagcact taaaatttac agattatcag
    61621 acctctcatt atgattcatt gaactttacc atattgactg gaattttgat caaattaaca
    61681 aatcaaccgt atgaaaaatt attgaccgat gatatcatcg aacctttagg attacagcat
    61741 acaaaatttt atgaccaagt caaaaattca tccaatcatg cactatccta tcaaatgtct
    61801 aaagaggatg attactttaa accactgaca gaatctgaaa cagagatcca taacgaatta
    61861 gggactggta atatcagtat gtccgtctat gatttaaata cgctgtttac acaagtttta
    61921 tctggaaaac tagtttccaa agacttactc ttctcattat ggcgtgaaaa tgcgaatgga
    61981 catccatata gtggtggggt atatagcgga gataattata tcctgtcgca aggaaatatc
    62041 gactcttttg atacatctgc tatatttagc aaagattcac aggatgctat catcatggaa
    62101 agcaatataa aagctgacaa aactatcaaa ctgcctactg ctgatctaag aaaccaaatc
    62161 tatgacctga tggaaggaac tactaagttg gcaaatagct gatatgatga aaagatagca
    62221 aatactactt ctttgaatag catttgctta aaaaaagtac tcgactaaac gtaggaaaga
    62281 gtgtgggaca aatctaaaaa tcaatttttg ctacagactt taattccaaa taaacagaac
    62341 agaaagaggt gttcttcttt ctgttctgtc caaattattc taagatttct ctttaccaac
    62401 attatttgac aaagggccta ttaaatctaa aatagtatct ttagattgct aaaatatgtg
    62461 ctagcaaatc attcatggct tgttcttatt tttaatttta tgcaataaaa aagaagaaca
    62521 attagcgtga ttatagaaat aaataatgag attttagaaa aaaatgtgta tgaatacctg
    62581 attgttactt tatttattcc actttcactt tttactgtaa caaaaggacc tccttgtttt
    62641 agtactgtag cattttttga attaacagta accgttaatc ctttataccg gaatacaggt
    62701 aaaactattg ttttgccttt tgaagattta aacgtaaatg taatttcatc tttatttgga
    62761 gaaaagctac tataaataac ttgaccatct tctgtaactt gatggttttt caaaagcaat
    62821 tgatcttttt tatagcctat aggtacataa tcaaaattct caaattcttc aagtttattt
    62881 tccatttgtt cattagaata agttccttga aaattcattt ctttcaccat gttaactact
    62941 gatgaataat ttaaaattga aatagctaat attaataata gtataaaatt tcttctcctt
    63001 gtataagaaa atttttccaa ctgtggagaa ataaatagag atactgaaaa agatatgaac
    63061 aatgttgaaa atatcataaa acgccaagga gcttgaataa tatttaaagg agtattttgt
    63121 aaggcaaacc atggaaatat ttttgtacaa agtagaatag atattatacc cattatataa
    63181 gtgattttag ataagattcc taattttt
//
LOCUS       DANJMI010000013        61702 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000013 DANJMI010000000
VERSION     DANJMI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.013"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(122..499)
                     /locus_tag="PWU81_000973"
     CDS             complement(122..499)
                     /locus_tag="PWU81_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109699.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            755..1483
                     /locus_tag="PWU81_000974"
     CDS             755..1483
                     /locus_tag="PWU81_000974"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL1109700.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1483..1737
                     /gene="purS"
                     /locus_tag="PWU81_000975"
     CDS             1483..1737
                     /gene="purS"
                     /locus_tag="PWU81_000975"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDL1109701.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1739..2410
                     /gene="purQ"
                     /locus_tag="PWU81_000976"
     CDS             1739..2410
                     /gene="purQ"
                     /locus_tag="PWU81_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HDL1109702.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2411..4633
                     /gene="purL"
                     /locus_tag="PWU81_000977"
     CDS             2411..4633
                     /gene="purL"
                     /locus_tag="PWU81_000977"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDL1109703.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4618..6057
                     /locus_tag="PWU81_000978"
     CDS             4618..6057
                     /locus_tag="PWU81_000978"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL1109704.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6080..7132
                     /locus_tag="PWU81_000979"
     CDS             6080..7132
                     /locus_tag="PWU81_000979"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL1109705.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7129..7707
                     /gene="purN"
                     /locus_tag="PWU81_000980"
     CDS             7129..7707
                     /gene="purN"
                     /locus_tag="PWU81_000980"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL1109706.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7704..9239
                     /gene="purH"
                     /locus_tag="PWU81_000981"
     CDS             7704..9239
                     /gene="purH"
                     /locus_tag="PWU81_000981"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL1109707.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9255..10529
                     /gene="purD"
                     /locus_tag="PWU81_000982"
     CDS             9255..10529
                     /gene="purD"
                     /locus_tag="PWU81_000982"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL1109708.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10651..11565
                     /locus_tag="PWU81_000983"
     CDS             10651..11565
                     /locus_tag="PWU81_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL1109709.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11698..13263)
                     /locus_tag="PWU81_000984"
     CDS             complement(11698..13263)
                     /locus_tag="PWU81_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL1109710.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13256..14707)
                     /locus_tag="PWU81_000985"
     CDS             complement(13256..14707)
                     /locus_tag="PWU81_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL1109711.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14721..15737)
                     /locus_tag="PWU81_000986"
     CDS             complement(14721..15737)
                     /locus_tag="PWU81_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1109712.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15957..17045
                     /locus_tag="PWU81_000987"
     CDS             15957..17045
                     /locus_tag="PWU81_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HDL1109713.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17099..18625
                     /locus_tag="PWU81_000988"
     CDS             17099..18625
                     /locus_tag="PWU81_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL1109714.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18627..19832
                     /locus_tag="PWU81_000989"
     CDS             18627..19832
                     /locus_tag="PWU81_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1109715.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20089..21066
                     /gene="guaC"
                     /locus_tag="PWU81_000990"
     CDS             20089..21066
                     /gene="guaC"
                     /locus_tag="PWU81_000990"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDL1109716.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21330..22754
                     /locus_tag="PWU81_000991"
     CDS             21330..22754
                     /locus_tag="PWU81_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL1109717.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23215..24324
                     /gene="pdhA"
                     /locus_tag="PWU81_000992"
     CDS             23215..24324
                     /gene="pdhA"
                     /locus_tag="PWU81_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDL1109718.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24327..25304
                     /locus_tag="PWU81_000993"
     CDS             24327..25304
                     /locus_tag="PWU81_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDL1109719.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25329..26972
                     /locus_tag="PWU81_000994"
     CDS             25329..26972
                     /locus_tag="PWU81_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDL1109720.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26980..28386
                     /gene="lpdA"
                     /locus_tag="PWU81_000995"
     CDS             26980..28386
                     /gene="lpdA"
                     /locus_tag="PWU81_000995"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL1109721.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28548..28823
                     /locus_tag="PWU81_000996"
     CDS             28548..28823
                     /locus_tag="PWU81_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDL1109722.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28829..29596
                     /locus_tag="PWU81_000997"
     CDS             28829..29596
                     /locus_tag="PWU81_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDL1109723.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29764..31593
                     /gene="typA"
                     /locus_tag="PWU81_000998"
     CDS             29764..31593
                     /gene="typA"
                     /locus_tag="PWU81_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL1109724.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31854..33626
                     /gene="recQ"
                     /locus_tag="PWU81_000999"
     CDS             31854..33626
                     /gene="recQ"
                     /locus_tag="PWU81_000999"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL1109725.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33740..34042
                     /locus_tag="PWU81_001000"
     CDS             33740..34042
                     /locus_tag="PWU81_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDL1109726.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34045..35208
                     /locus_tag="PWU81_001001"
     CDS             34045..35208
                     /locus_tag="PWU81_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL1109727.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35234..38662
                     /locus_tag="PWU81_001002"
     CDS             35234..38662
                     /locus_tag="PWU81_001002"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDL1109728.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38683..39807
                     /locus_tag="PWU81_001003"
     CDS             38683..39807
                     /locus_tag="PWU81_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109729.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39797..40234
                     /locus_tag="PWU81_001004"
     CDS             39797..40234
                     /locus_tag="PWU81_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL1109730.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40218..40556
                     /locus_tag="PWU81_001005"
     CDS             40218..40556
                     /locus_tag="PWU81_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDL1109731.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40675..41235
                     /gene="rsmD"
                     /locus_tag="PWU81_001006"
     CDS             40675..41235
                     /gene="rsmD"
                     /locus_tag="PWU81_001006"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL1109732.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41232..41723
                     /gene="coaD"
                     /locus_tag="PWU81_001007"
     CDS             41232..41723
                     /gene="coaD"
                     /locus_tag="PWU81_001007"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL1109733.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41713..42756
                     /locus_tag="PWU81_001008"
     CDS             41713..42756
                     /locus_tag="PWU81_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDL1109734.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42915..43643
                     /locus_tag="PWU81_001009"
     CDS             42915..43643
                     /locus_tag="PWU81_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL1109735.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43759..44004
                     /locus_tag="PWU81_001010"
     CDS             43759..44004
                     /locus_tag="PWU81_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL1109736.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44001..44219
                     /locus_tag="PWU81_001011"
     CDS             44001..44219
                     /locus_tag="PWU81_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDL1109737.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44355..44894
                     /locus_tag="PWU81_001012"
     CDS             44355..44894
                     /locus_tag="PWU81_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDL1109738.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44887..47160
                     /locus_tag="PWU81_001013"
     CDS             44887..47160
                     /locus_tag="PWU81_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL1109739.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47198..48244
                     /gene="holA"
                     /locus_tag="PWU81_001014"
     CDS             47198..48244
                     /gene="holA"
                     /locus_tag="PWU81_001014"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL1109740.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48333..48587)
                     /locus_tag="PWU81_001015"
     CDS             complement(48333..48587)
                     /locus_tag="PWU81_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL1109741.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48987..49256)
                     /locus_tag="PWU81_001016"
     CDS             complement(48987..49256)
                     /locus_tag="PWU81_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109742.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49726..50181)
                     /locus_tag="PWU81_001017"
     CDS             complement(49726..50181)
                     /locus_tag="PWU81_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL1109743.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50319..52247)
                     /locus_tag="PWU81_001018"
     CDS             complement(50319..52247)
                     /locus_tag="PWU81_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL1109744.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52444..53277)
                     /locus_tag="PWU81_001019"
     CDS             complement(52444..53277)
                     /locus_tag="PWU81_001019"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDL1109745.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53469..54173)
                     /locus_tag="PWU81_001020"
     CDS             complement(53469..54173)
                     /locus_tag="PWU81_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL1109746.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54196..54822)
                     /locus_tag="PWU81_001021"
     CDS             complement(54196..54822)
                     /locus_tag="PWU81_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL1109747.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55148..57523
                     /locus_tag="PWU81_001022"
     CDS             55148..57523
                     /locus_tag="PWU81_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL1109748.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57547..58038)
                     /locus_tag="PWU81_001023"
     CDS             complement(57547..58038)
                     /locus_tag="PWU81_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL1109749.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58225..59145
                     /locus_tag="PWU81_001024"
     CDS             58225..59145
                     /locus_tag="PWU81_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL1109750.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59299..59643
                     /locus_tag="PWU81_001025"
     CDS             59299..59643
                     /locus_tag="PWU81_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDL1109751.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59833..60333)
                     /locus_tag="PWU81_001026"
     CDS             complement(59833..60333)
                     /locus_tag="PWU81_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1109752.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60861..60944
                     /locus_tag="PWU81_001027"
     tRNA            60861..60944
                     /locus_tag="PWU81_001027"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60895..60897,aa:Leu,seq:cag)
     gene            complement(61268..>61702)
                     /locus_tag="PWU81_001028"
     CDS             complement(61268..>61702)
                     /locus_tag="PWU81_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDL1109753.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        20233 a        10662 c        13216 g        17591 t
ORIGIN      
        1 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
       61 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
      121 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
      181 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
      241 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
      301 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
      361 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
      421 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
      481 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
      541 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
      601 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
      661 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
      721 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
      781 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
      841 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
      901 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
      961 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
     1021 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
     1081 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
     1141 tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag
     1201 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
     1261 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
     1321 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
     1381 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
     1441 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
     1501 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
     1561 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
     1621 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
     1681 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
     1741 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
     1801 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
     1861 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
     1921 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
     1981 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
     2041 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
     2101 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
     2161 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
     2221 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
     2281 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
     2341 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
     2401 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
     2461 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
     2521 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
     2581 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
     2641 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
     2701 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
     2761 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
     2821 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
     2881 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
     2941 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
     3001 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
     3061 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
     3121 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
     3181 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
     3241 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
     3301 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
     3361 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
     3421 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
     3481 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
     3541 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
     3601 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
     3661 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
     3721 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
     3781 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
     3841 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
     3901 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
     3961 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
     4021 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
     4081 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
     4141 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
     4201 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
     4261 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
     4321 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
     4381 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
     4441 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
     4501 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
     4561 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
     4621 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
     4681 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
     4741 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
     4801 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
     4861 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
     4921 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
     4981 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
     5041 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
     5101 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
     5161 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
     5221 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
     5281 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
     5341 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
     5401 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
     5461 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
     5521 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
     5581 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
     5641 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
     5701 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
     5761 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
     5821 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
     5881 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
     5941 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
     6001 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
     6061 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
     6121 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
     6181 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
     6241 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
     6301 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
     6361 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
     6421 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
     6481 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
     6541 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
     6601 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
     6661 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
     6721 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
     6781 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
     6841 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
     6901 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
     6961 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc aagaatcgaa
     7021 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
     7081 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
     7141 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
     7201 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
     7261 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
     7321 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
     7381 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
     7441 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
     7501 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
     7561 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
     7621 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
     7681 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
     7741 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
     7801 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
     7861 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
     7921 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
     7981 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
     8041 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
     8101 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
     8161 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
     8221 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
     8281 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
     8341 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
     8401 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
     8461 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
     8521 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
     8581 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
     8641 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
     8701 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
     8761 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
     8821 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
     8881 agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc aaacatgtga
     8941 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
     9001 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
     9061 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
     9121 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
     9181 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
     9241 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
     9301 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
     9361 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
     9421 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
     9481 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
     9541 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
     9601 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
     9661 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
     9721 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
     9781 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
     9841 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
     9901 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
     9961 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    10021 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    10081 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    10141 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    10201 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    10261 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    10321 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    10381 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    10441 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    10501 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    10561 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    10621 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    10681 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    10741 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    10801 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    10861 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    10921 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    10981 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg
    11041 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    11101 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    11161 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    11221 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    11281 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    11341 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    11401 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    11461 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    11521 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    11581 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    11641 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    11701 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    11761 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    11821 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    11881 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg
    11941 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    12001 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    12061 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    12121 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    12181 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    12241 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    12301 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    12361 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    12421 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    12481 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    12541 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    12601 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    12661 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    12721 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    12781 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    12841 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    12901 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    12961 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    13021 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    13081 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    13141 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    13201 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    13261 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    13321 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    13381 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    13441 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    13501 ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac
    13561 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    13621 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    13681 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    13741 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    13801 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    13861 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    13921 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    13981 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    14041 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    14101 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    14161 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    14221 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    14281 gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta ttgatcagct
    14341 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    14401 ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt tcttctagga
    14461 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    14521 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    14581 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    14641 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    14701 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    14761 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    14821 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    14881 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    14941 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    15001 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    15061 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    15121 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    15181 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    15241 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    15301 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    15361 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    15421 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    15481 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    15541 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    15601 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    15661 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    15721 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    15781 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    15841 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    15901 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    15961 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    16021 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    16081 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    16141 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    16201 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    16261 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    16321 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    16381 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    16441 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    16501 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    16561 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    16621 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    16681 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    16741 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    16801 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    16861 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    16921 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    16981 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    17041 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    17101 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    17161 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    17221 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    17281 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    17341 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    17401 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    17461 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    17521 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    17581 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    17641 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    17701 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    17761 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg
    17821 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    17881 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    17941 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    18001 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    18061 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    18121 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    18181 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    18241 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    18301 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    18361 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    18421 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    18481 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    18541 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    18601 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    18661 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    18721 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    18781 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    18841 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    18901 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    18961 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    19021 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    19081 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    19141 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    19201 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    19261 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    19321 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    19381 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    19441 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    19501 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    19561 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    19621 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    19681 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    19741 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    19801 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    19861 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    19921 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    19981 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    20041 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    20101 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    20161 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    20221 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    20281 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    20341 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    20401 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    20461 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    20521 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    20581 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    20641 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    20701 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    20761 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    20821 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    20881 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    20941 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    21001 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    21061 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata
    21121 agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag
    21181 atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag
    21241 tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag
    21301 aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac
    21361 cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc
    21421 ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca
    21481 ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca
    21541 gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc
    21601 ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag
    21661 caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc
    21721 ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag
    21781 aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta
    21841 aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa
    21901 tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt
    21961 ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga
    22021 tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga
    22081 aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac
    22141 gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg
    22201 tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc
    22261 cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg
    22321 gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg
    22381 agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg
    22441 atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt
    22501 ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga
    22561 ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc
    22621 ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca
    22681 gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt
    22741 ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct tggatttaaa
    22801 tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact aagaaaaaac
    22861 cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa caaaactgta
    22921 taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa aatagaaaaa
    22981 atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat gctgttaata
    23041 tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt gtatttctca
    23101 taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt tattcactgt
    23161 ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg acaaatggca
    23221 aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc agatttccca
    23281 gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt acctgattta
    23341 tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt attagaccaa
    23401 cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa
    23461 gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct tcttccaggt
    23521 tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc ttttttatgg
    23581 tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct accaccacaa
    23641 attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt gaagaaacga
    23701 aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca aggtgatttt
    23761 tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta tatccaaaac
    23821 aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa
    23881 aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc gcttgcagta
    23941 tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc agtcctaatc
    24001 gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc aacccgctac
    24061 cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg cttccgtaaa
    24121 tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact
    24181 aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca aaaggtttca
    24241 gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt
    24301 tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa gcaatcacag
    24361 atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg
    24421 ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc ggagaagacc
    24481 gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt
    24541 tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc gaagcaatgg
    24601 acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact agaaatctac
    24661 ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg cattcggata
    24721 atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct tccaaccctt
    24781 atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt gttttcctag
    24841 aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct tacgaagttc
    24901 ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc acatacggag
    24961 caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat atatctgttg
    25021 aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt caatccgtgg
    25081 aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc gttgccgcgc
    25141 aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca atcggacgag
    25201 tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg
    25261 caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa
    25321 acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc gcagaaggtg
    25381 aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat acattactag
    25441 aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga acggttaaaa
    25501 atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta gaaatcgatg
    25561 caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc caaactccag
    25621 ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca agcgaaggag
    25681 tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa atcgtcaaat
    25741 ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa gttcaaaatg
    25801 acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac gtgattgttc
    25861 cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct cctggacaca
    25921 atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat
    25981 caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg gctatgccat
    26041 ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact gcaactggaa
    26101 aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg
    26161 caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct cctgcagcag
    26221 aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac ttggaagaac
    26281 gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac agtaagcata
    26341 cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg gataatcgga
    26401 aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg ccatatgtcg
    26461 tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct atcgacgatg
    26521 caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact gatacggatc
    26581 acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt gctattgcag
    26641 acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca gaagatatgc
    26701 gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg tttacaccag
    26761 tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa caaccaatcg
    26821 taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc
    26881 accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa cgtcttttag
    26941 ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg agattttgct
    27001 gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc tgccatccgc
    27061 gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg cggtgtttgt
    27121 ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca ttatcaagag
    27181 tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa
    27241 acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt tggtttccta
    27301 ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca
    27361 ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc aatcatcgca
    27421 acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt gttagattct
    27481 actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg tggcggtgtg
    27541 attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac gattcttgaa
    27601 ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt agaagatgat
    27661 ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac
    27721 ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt tactgctgat
    27781 tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt agaacaagcg
    27841 ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg tacgaacgta
    27901 cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca caaagccagt
    27961 tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt agattataaa
    28021 gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat gacgatcaaa
    28081 gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc agggaacggt
    28141 cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac gatcgaagat
    28201 aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta
    28261 gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat tcatcctcat
    28321 ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt accaatccat
    28381 atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta
    28441 gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg gtttattacc
    28501 acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg caagaatatc
    28561 agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg aagatgtggg
    28621 aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg aagacttatc
    28681 agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga cgggaatttg
    28741 aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca
    28801 aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata tcaagtcctg
    28861 gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa
    28921 atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt ttctaatcga
    28981 aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag ggtgtaatca
    29041 attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca ccttgaattt
    29101 tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg gcgttggaaa
    29161 gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa aaggaatagg
    29221 cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat cggaaggctt
    29281 acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag aaattgcgga
    29341 aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg caatcttgaa
    29401 aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag cgggaaatgt
    29461 cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt tgacatttga
    29521 aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg cagacctttt
    29581 gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa
    29641 agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa aagtctgcta
    29701 taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa ggagttagtt
    29761 acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga
    29821 aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc acatacgcaa
    29881 ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta
    29941 gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga tactccagga
    30001 cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg
    30061 gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa aaaagcactg
    30121 gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc tgctcgtcct
    30181 gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga tgatgatcag
    30241 ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt gtcagatgat
    30301 ccagcagatc aagaaccaac catggcacct atttttgata caattattga aaaaatccct
    30361 gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact tgactacaac
    30421 gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa agtaggagac
    30481 caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac gaaattattt
    30541 ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga cttgattgct
    30601 gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat
    30661 gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt agttaataat
    30721 tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga agaacgtttg
    30781 atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc tccagatgca
    30841 tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa catgcgccgt
    30901 gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat cgacggtgta
    30961 aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat gggtagtgtt
    31021 atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac aggaaacgga
    31081 caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc aactgaattc
    31141 ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata tctgccaatg
    31201 cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa
    31261 gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt
    31321 acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga tttgactgtc
    31381 aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga ccaaacatct
    31441 gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt gaatgatgat
    31501 gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt agagaaaaat
    31561 gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag agagaaacca
    31621 gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt ggagaagtca
    31681 tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa
    31741 aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga aaggagatca
    31801 acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg
    31861 acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa gaaattatcc
    31921 agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt gggaaatcaa
    31981 tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg tcccctttaa
    32041 tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc gccactttta
    32101 tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc gatagacagc
    32161 agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt atccaagcaa
    32221 tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc tctcagtggg
    32281 ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa ctgccaaatc
    32341 gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct gacataaaaa
    32401 gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga gaaaatctac
    32461 gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat ttaaagatca
    32521 acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac cgtctctatc
    32581 atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta aatgagaatg
    32641 agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt gtagcgacaa
    32701 atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat tatcaaattc
    32761 ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat ggtctttcca
    32821 gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt tttatccagc
    32881 agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa gcgatgacag
    32941 aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt ggcgagacaa
    33001 gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta gaagtaacga
    33061 cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa
    33121 tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta ggttttggac
    33181 atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg
    33241 aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct gttttgaaag
    33301 taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga aaggaaccca
    33361 agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg ctgcgggaac
    33421 tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt tcagattcca
    33481 cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt cagataaaag
    33541 gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg ataaaaaatg
    33601 cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa taaaaacaac
    33661 tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat taaggaatta
    33721 gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa
    33781 tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc tctgtatctc
    33841 acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt cccggcaagt
    33901 cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga tgttaagtga
    33961 gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt atgataaaaa
    34021 agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca tctattagac
    34081 tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat ggtatatagt
    34141 tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt cattaaccag
    34201 agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat gaaaacagat
    34261 gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat tttgctgctg
    34321 attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca aattgcagga
    34381 ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct gtctctgact
    34441 ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa acgtccactt
    34501 gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg taatgcggca
    34561 gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta tgtgtatact
    34621 ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct gatcaatatc
    34681 acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt tgctatttac
    34741 caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg ctactatgcc
    34801 atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa aaaaggattt
    34861 ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact gggagtgatt
    34921 atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt ccttgtaggt
    34981 atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc aatgtttttg
    35041 atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac agggatcacg
    35101 ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg tgtcggcttt
    35161 gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata aaaaaagatg
    35221 taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt
    35281 atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag aagaagatga
    35341 atattccgta caccgtttca aagcggatga agcatattta gtaggacaag gaaaaaaacc
    35401 gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat gcggagctga
    35461 cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga
    35521 agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt ttggagataa
    35581 aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag gcacagatgg
    35641 accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt atccgattat
    35701 gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg atgaaaagag
    35761 tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga
    35821 agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga ttctcgggga
    35881 tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc gccgtcacca
    35941 aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg
    36001 ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt
    36061 tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc aagtggaaca
    36121 tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt tgattgctca
    36181 aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta aattaaatgg
    36241 ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt taccagacac
    36301 agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg atgtcggaaa
    36361 tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa aagtatgtac
    36421 gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga aagaatttag
    36481 aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac atcctgaatt
    36541 ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat ttgaatttcc
    36601 tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg
    36661 ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga
    36721 tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga agggtgccga
    36781 tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata cgacatttag
    36841 agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca agcagattgc
    36901 acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg ggggagcaac
    36961 atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga gaaaaattcg
    37021 ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta
    37081 ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag cgcaaggaat
    37141 cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa aaagtatcca
    37201 agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg gcgatatcaa
    37261 cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa aggaactaga
    37321 acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta aaccgcaggc
    37381 agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc atctgcatac
    37441 tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag caggagtaga
    37501 catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta gcatgagcag
    37561 cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg aaaatgcaca
    37621 gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg aaaacggctt
    37681 gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt attcgaatct
    37741 acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca aacaaatgta
    37801 tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat ctaaagtagt
    37861 tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata tttatgaaaa
    37921 aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag aactagggca
    37981 acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca gacctgcatt
    38041 gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt
    38101 ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt tgatgtatcc
    38161 gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta cattactgga
    38221 cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg
    38281 cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg aagggaatcg
    38341 cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag ataattcgat
    38401 catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac aaattggagc
    38461 aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg
    38521 tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag cacgttttga
    38581 cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg gtgacctttt
    38641 gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag actgggtttg
    38701 ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt cttgtttccg
    38761 ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat gacaacgtgg
    38821 aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc gatcgatcag
    38881 ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg ttttgaaaca
    38941 agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca agacagcaga
    39001 gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg
    39061 atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac ttttgagtac
    39121 catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg tccgctaatt
    39181 gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa cggcctattt
    39241 tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca agtgtttggt
    39301 gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat caatcgcgtt
    39361 aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga acttcccata
    39421 tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga ttttttgaca
    39481 aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc gctaggcagt
    39541 gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt aatcagtaaa
    39601 caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc tactttttcg
    39661 tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc gggaacggat
    39721 tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga agaaaacgga
    39781 gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg atcaatgcct
    39841 ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg aacataaaca
    39901 gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga
    39961 agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg aaaaaaaacg
    40021 agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag ctttccgata
    40081 tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg ccgaagaagt
    40141 ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa aacactttgg
    40201 atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca aaacgacgcg
    40261 ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt
    40321 tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa gagacattgg
    40381 aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa cgttcttacc
    40441 gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta ttaaaagacg
    40501 aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat ctttaaaaat
    40561 ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt ttgatcattg
    40621 tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga gtagatgcgg
    40681 ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga
    40741 ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata ttttgatgga
    40801 gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc agtatctaga
    40861 ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat catcaaagaa
    40921 aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga tgcggacaaa
    40981 gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct tgatccgcca
    41041 tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg acttttttct
    41101 aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga aactgtggct
    41161 ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat ctatagaaaa
    41221 gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt acaaaaggtc
    41281 acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt ggtgttttta
    41341 tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg atcacaaaag
    41401 ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa
    41461 cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc aaagactttg
    41521 aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg gaaactgtct
    41581 tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa gaagtactgc
    41641 actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct ttagctggaa
    41701 agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat catatttgga
    41761 atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc accgggagcc
    41821 acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg ttctgggtct
    41881 ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa
    41941 atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc gacaaatgaa
    42001 gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc aatcgaacaa
    42061 gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata tgtcatgagt
    42121 gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt aacaaaagta
    42181 gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag ccaaaaagtt
    42241 ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc ttctggtgat
    42301 ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac tgaccatacc
    42361 gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg gccatcagcg
    42421 ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt gcgacatggt
    42481 aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg aatcggagga
    42541 atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt tgcaccagat
    42601 gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa
    42661 gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta
    42721 caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa agaggggttg
    42781 tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa
    42841 acaaaagaaa aaattgacgt aattagatgt tgaagggacg attttgccag agctcactgt
    42901 ttcttcaatt ttaaatgaaa ggagtaatga atgattttta ctggtcattc tttgaaaaaa
    42961 gaacaatgat agaaaaaatc agcaagaaaa tactagcttc acccaaattg atcatttgga
    43021 tgtctatgat agttcttatc ttttgtatcg caagtggttt tttattatca aatttgctat
    43081 tttatagaga aacagaagag actgctgcaa aagaaatact ttcttcgttt agttccagtg
    43141 gagaagatcc atcagctgaa ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt
    43201 acaccgatat caaaggaagt gtgaaggagc ctggtatcta ttccttttct tcagaagaac
    43261 gggtctatga tgtactaaag cgtgcaggtg gattattaga agaagcggat agcgatcgaa
    43321 tcaacttttc tgcgaagata gaagatcagc aagtcctata tatccctgct gtaggtgaag
    43381 agctgccaga acatcttgat cagttggcat cctcggaagg aaaacagcct acagcagatt
    43441 ccgagcctcc caaaataaat atcaatacag ccactccatc agaacttcag cagattcctg
    43501 ggatcggatc agtcaaagcg caagaaatca ttcgatttag agaagaaaac ggatccttcc
    43561 agaaagtgga agacctgcag gagatttctg gtatcggtga aaagacagtt gaaaaactaa
    43621 aaaacttcgt tacaattaaa tagaatgatt caaaatcgac tggcacgagc aataaatgga
    43681 cgctaatgga tactaagaat aatccaattc taagagcttg gcaggacgag gaggtagtat
    43741 ttttggatag gaaatagctt gattgttcgc aaagaaacat taaaaaaacc aatgctgaat
    43801 gtctatctcc aaaataaaat atctgggatt catattatga atacggctgt cagcggcaat
    43861 aacagtcaag ctctacgaga acgctttgcc aaagatgtac ttagctatac agcagataaa
    43921 gtatttattc tcattgggac gaatgatcta gcagagcata aacaactttc taaagaaacg
    43981 taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa agactatagt tctgatcatg
    44041 ctttctttat cacgccacca gcagtcgatg agacaaaaca aaaatatcgg aataacaagt
    44101 tgatcaaaga atatatctct gttctcacag aagtagcaaa acgccatgat tgtgaagtat
    44161 cgatttttat acggaatttc ttgaacaaat ggattcgaag aatttttata atgggctaat
    44221 ggatgacgga cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg
    44281 ttatatttaa agaatatttt gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg
    44341 gaggaatata aaacatgtta gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag
    44401 ttactgataa gtcacataat gaacgcattc cttgggacca gtactttatg gctcaggcag
    44461 tactgttatc tttacgtagt acgtgtacac gtttagaagt aggagcgaca ctcgttcgag
    44521 aaaaaaggat cattgctggc ggctataatg gagcagtcag tggagatgtc cactgcatag
    44581 atgaagggtg ttacgtcgtg gatggacact gcttgcgaac gatccatgca gagatgaatg
    44641 cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc tatgtcacac
    44701 attttccatg tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt
    44761 atctccatga ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat
    44821 ctgtacacca agttgcttta gactcacagc atttttcaaa acttcaaaat gaattaacag
    44881 gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt tccggcaatt
    44941 acgtcgatga ttatctgttt gttgtattac actgacaatt ggggttatcg tttgcttctt
    45001 gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt
    45061 ttgtgtagca taacggtatc catttcttat aggctacaag agtattcatt tttagaggaa
    45121 aaaacaacat ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa tggtaatttt
    45181 ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc tccagctttc ttatcaagta
    45241 aacacagaag cagaattgaa aacatggctt aacttccgag gaaagggaat agaattgact
    45301 gctgaaggcg agttcatctc tccagcaaaa aagcggaatc tttataattt tgaccaagaa
    45361 gcttacttca ggaaccatag aatttctgga cggttccaaa ttcaaaagat tgaacagatc
    45421 accatgcaaa atcactggcg aaactggctt cagagaaaaa gaggatcttt tatctccttt
    45481 gtccaatctg aatttccgca aaaaacaagt gtatatatta attctttatt atggggctat
    45541 aaagatgcag gattcagaga accagaagaa atttttaggg agagtggttt gctgcatctg
    45601 ttcagtctat cgggtctaca catccaattt tatttagggt ggctgtatta tttgtttcga
    45661 cgtatcggat ggccactaca cgtcagtatt attccgttga gtctactaac tatctgttat
    45721 gtttctattg caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga
    45781 ttgctagtca aattgctgaa taagcattat tccaccatgg acttatttgc agttgtcttt
    45841 tggcttcttt tactatttga tcctcgattt ctttttcatg cgggtggaca gcttagtttt
    45901 ttcatgagtt ttttgtttat cctgatttct tttgatggag attggttgaa aaaaacggcc
    45961 tctcacgtcc tgtttactat gatcacgatg ccgttgattg tatggcattt ttatgagtgg
    46021 cctattttag gtagtctttt tacattactg attgcaccgg tattcaagtt tctttttctt
    46081 cctggtgtgt tatttctcac actcttcaat cgctggctgc cggaaacttt acttttgctg
    46141 atagaagagg gattaatctt atttgagcga ataatcgatt tttcttcagg gtcgtctctc
    46201 attataggac gtttgcccct tgtgctatct gggatgtata ttttcggatt actgatttgt
    46261 atggataggt tgaaagaaca acctgtgaaa cacttgtttt atgttggtct tttttctttt
    46321 atttttttga ctttgccatt tttacgcttt tcttcaacga tcgcttttat cgatgtcggt
    46381 caaggagaca gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt gatcgatact
    46441 ggaggtcagt tacactttga aagagaaaag tgggcgcaag gtatgatacg tcctcctgct
    46501 gaaagaaata tcgttccata tctaaaaggt caggggataa gtgtaatcga taaactcatc
    46561 ttgacacacg acgatacgga tcatgtggga gaattaccca cgttatctca gcattttact
    46621 atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg agccattaca aaggcattta
    46681 tctgaagctc aaaaaaatgg tactgagatc attgaaatca aacatgggga caggatcagc
    46741 ggttattatg atctttatgt attgactcct tttgaaaagg gagagggaag aaatgaagat
    46801 tcgattggtc tatggatgga atataacaat aggcgctttt tatttttagg agatttgaat
    46861 caagcaatgg aaaaacaatt gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa
    46921 ttaggtcatc acggtagccg tacgtcaagt aacacagatt ttctttccca tattgaagca
    46981 aaacatggga ttatatcctg cggcgtaaat aatcgttatg gtcacccgca cagcgaagta
    47041 ttggagacac tagaagaaaa tcagataaaa acatggcgaa cggatcaaca aggaatgatc
    47101 acctatcgct ggcatccagt ttttcatcct catgggctag tggaaacaat gatagactag
    47161 taattctcat ctgctcatgg taggataatt tttgaaagtg gtgagtaatt tgagtataca
    47221 agcagagtta caaaaaatac gtaaagaaaa acttgcatct tgttacttga ttttaggaac
    47281 agaaaagttt ctccaagatc aagttcggac agaaattgtc aaaaaaatcc aattagaggg
    47341 cgaagatgac ttaaattttt taagttttga catggaaaat gcaagtttag atgaggtagt
    47401 agctgaagca gagacgttgc cgtttttcgg agatcaacgg ttagtattcg tagagaatcc
    47461 ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg atttattgac
    47521 ggcttatttg aaagaaccat tagaatcgac tgtattagta ttctttgcgc catatgaaaa
    47581 gttggatgaa cggaaaaaag taacgaaaca attgaaaaaa acagcaatta tcatcaatgt
    47641 ccaacagttg aatgaaaaag aagtacgtca atatctgatg aataccttag agaattcctc
    47701 aatcaaaatg gaacgaagtg caattgattt attcttgagg ttgaccgact tagatttatc
    47761 caaaatgatg agagagttac agaaattggt cctttatgga caaaatcaac agcaaatcac
    47821 ggtaagagaa gtagaagaat tagttcctaa aacgttagaa cataatattt ttgacatgac
    47881 ccaatatatt ttaaaaggga agacggaaca agctttgcgt ttatacgaag atttagttat
    47941 gcaaggggaa gaaacaatca aaattaatgc gattttgctt tctcaattac gtttattttt
    48001 acagacaaaa tttcttgtaa agattggtta tcagcaggca aatattgctg aaacattgaa
    48061 aatccatcca tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg atgaacattt
    48121 gctggtacag ttgtttgatc aattagttga gatggattat caaatcaaaa cgggacaaat
    48181 cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca gggcagttgt taagaaatcg
    48241 ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg
    48301 acaaataaaa aaatcggccg aagccgattc ttttatttag cgattttttt gcttaaacga
    48361 gatttgtcgc ggttagcttt gtttttgtgg attagacctt ttgattcagc catatcaata
    48421 gctttaacag cttctttata taacgcatct acgttgtcag cacctgaagc tacagcatct
    48481 tcaaattttt tgattgcagt acgcatagcg cttgtttgag atgaattttt cacattagcg
    48541 ttttcgcttg tacgaacacg tttgattgca gattcaatgt ttggcattag tttcacctcc
    48601 gataacttaa gttcttacgc gtgttaccgc ataattcaac attcattatt atacctaaag
    48661 gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc ttaacagtct tttattagga
    48721 ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg
    48781 gaggcaatag cgtcaaacca aactagattt ttatttatct atttaaacaa agtttgaatg
    48841 ggaatgtttg gtactaagtt attatataac aaagagaacc gtttgcaaag ttttttttga
    48901 aaagggatac gattccctgt tgttctttgt ttttctatta agaaactgca gttattttat
    48961 aaccagtatc ctttgatcag taaaggctat tctggtgtac cgattatttt aatgtattca
    49021 tcgatatttt ttatcaacat tttttttatg accaactggt tccgacggtt ccatttgcca
    49081 cttacttcca gcacatattt atttaggggc aaacgtaaga tatattttgc caaatagcga
    49141 tctttaagca ggccattgat cacacggtcc tcaagctgca agttgatccc agtcagcatc
    49201 attggctgtt cttgtaaaac gtacgtatca attaaaagac cttcaaaagt ttccacaagc
    49261 gtcttccttt cttttcaaag taacctgaaa acaggcacta attcttccat tttgatattc
    49321 gttgaagtgg atgcgatcat tctttctttt gtccactgta atctagtata acaaaaaaga
    49381 gcagaagagc caaaaagaaa aaagagcatc gaattctata ggaagtctca ttggcacggc
    49441 ctaaattatt acaaaacatt gattaaaaaa agcccctgca tcgcaggagc atcccctata
    49501 tcgttttaat aggtcaatca aaaagcagta tcaaacacat tttgtatcaa atcaggaaat
    49561 aatagataag ctaagcccag aatcaaaagt accgctagaa gaaaccagcc gattgttcct
    49621 atgaaatact ttaatttttc gaacattgta tcacctcgtt gtcagtatat cacggatcag
    49681 taaattgggg gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc
    49741 aaatacttgt aagatacagt aagtgatacc agaagcaatg accggtccgg cggcgattcc
    49801 tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc
    49861 agaagcaatc aatcctacac ccttggaaga taagacagcc actagcccac cgcaaaaaac
    49921 agctacccag ccaatagggg atttaaaggc ttctaataga tcacgtagac cgatttctcc
    49981 tgttgcgata gggattagaa tagccgctgt gatgatcgtc accccaatat tgattccttt
    50041 gccttgaagc caagggaaaa aggaacggga ggctggtatg agcttcataa tcaaaacaaa
    50101 agctgccgct atcatcagac tttgattttt agctaaaatt gctacaagta aaataattcc
    50161 taaaaaaagc caactttcca tgaaatatat tcctcacttt cttcgaaaca atcataacat
    50221 gtttttagaa agttacaaaa gtaggaattc atccttcaag acctattcat aagctgtttt
    50281 tttcataaaa taaacaaaat cataaaaaaa cactttgctt atttgacaga agacagccgc
    50341 gctttgtata gtggacaaga cttacatttt tttgaagaat cagaaagatc aggtaaaggt
    50401 cttagtaaac gcaagccatt cagaataacc aatattgtgc ttccttcgtg tcctacaact
    50461 ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag aataacaaga
    50521 atcgaaaaaa taatattttg tttgatgatc cgccgcattt ttaaagatag ccgatgactg
    50581 tagacaaatt tatttaagtc gcttttcatt aagacgatgt ctgcgatatc catagcggca
    50641 tctgtccctt gccccatagc gacacctaaa gaagcggctg ctaaagcagg cgcatcattg
    50701 actccatcac caaccatcgc atttacttgg taaagttcat tctctttttt gactagttgt
    50761 gttttttctt ccggagtaca gcttgcatag taatgttcaa tccctacttg ttcagcaatg
    50821 gcctttgctg tgccagaatg gtcgccggtt atcatacttg tatggatgtt ttgggattta
    50881 aagtaggaaa ttacttgttg ggtatttgct tttggaatat ctagcagacc taaaacagca
    50941 attaactgct gatctttaga aagataaatc acggttttgc cttgattttc caaacgttcg
    51001 atcatttccg caatctcttt gctgattatc atcgtctcag gatcatatgc gtgcttgcca
    51061 actttccatt gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat ttcttctatt
    51121 ggtaagtatt ggatttcttc aggtattgtg caatcgaaat attgcaaaat cgcatgagca
    51181 agtggatgcg tcgttttttg ttccatagca acaagaagtt gttgtgccaa atccttttca
    51241 tcaaagaaaa agctgtcagt tacaactggt tttcctttag tgagcgttcc tgttttatca
    51301 aaggcaatcg cttttaattc agaaagcttt tctaaatgga taccgccttt gattaaaatc
    51361 ccattttttg cagaatttga aagagcagct agagaagctg gtgtagcaga tgcgacaagc
    51421 gcacaaggag aagccacaac taataatacc attcctcgat aaaagctttc atcccaactc
    51481 caacctagga aaaagtgggg gagaaggatc attagaggaa cagcaagcaa aatcaatttt
    51541 acataattat tttcgattcg ttcgataaat gaagctgttt gagttggcgt attctgcgct
    51601 tcttctacta attggataat cttagcaaag acagtttcgg aactcgtttt tgttactcga
    51661 atcgtgatgg gatttccttg attcagcgtt cctgcataca tctcatcgcc taaatatttt
    51721 tctacaggca cagattcacc gcttaatgca gactcatcga tcgttgcctg tgtgctttct
    51781 aaaaagccat ctatcggaac agctgctcct ttagcgacaa ataccaagtt atccaccttt
    51841 agttgagaaa catctacttc ttgcatttgt ccattttctt ttaataattg ggctttttgt
    51901 ggttgaagat tcatcaaagc agtaatttct ttttgacttt tattagttgt gtattcctct
    51961 aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta attaccgatc
    52021 aaacaggcac caatagcagc taaagccatc aataaatcca cattcagtgt ccgatcattc
    52081 cacaattctt tcaagccttc cgatgtttgt ttatatccac cgatgataat ggcactgata
    52141 taaaaaagtg gggtgatggt aagagagaat cggtcgaaga taaaaccgaa gatcataaat
    52201 aaaaggcagc cgattgttgc tgcaaaagct tttttctgat tagtcatcgt tattagacct
    52261 ccttgtcaaa acaaggatag cataataaaa atgtaattga taataataac agctaaatga
    52321 gaacgataat ggttatcatt tggtgattaa gcttgatgtt gtttttatta tttataatga
    52381 ttattattaa agaaaataaa aaaccagaac atttctcaaa taagagaagt gttctggttt
    52441 tttctacatt gcaataaacc aataaccaat caaaatgata ccaaggataa tacgatacca
    52501 gccaaaagcg gtaaagtcgt ttttcttcaa atagcttagt aagaatttga tagctaagac
    52561 ggaaaccaca aaagatacga atgttcctac aagaagaaca acagtttctt ccattttgaa
    52621 tgaattccct tcagctaaat atttgacgat cttcaagccg cttgcgccaa acatgacagg
    52681 aatccctaag aaaaatgaaa actctgtagc gacaaaacgc gaagcaccaa tcaagataga
    52741 tcctaggatc gttgcgccag aacgggatgt tccgggaaca agtgccaaga cttggaataa
    52801 cccaacaaga agagctgctt ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt
    52861 gtttttgttg cgtttttcga caataataaa ggcaataccg taaacgatca aaacaatggc
    52921 aactggtaag aacttgtgga aatttgcttc taaccaatca tccaaaggca aaccaatgat
    52981 gatggctggt atgcaagcaa cgatcacttt aaaccaaagt gtccacgtat cttttttttc
    53041 taccgttgat ttacttggag aaaatgggtt taacttgtga aaataaagaa cgacgacagc
    53101 caagatcgcc cctaattgga taacgacatt gaacatcgac ataaattgtg aactggcatc
    53161 cagtttgatg aattcatctg ctaggatcaa atgaccagta ctactgatgg gcagccattc
    53221 cgttattcct tcaataatac cgagaatcac tgctttgata atatttgcaa aaaacataaa
    53281 ttccttcctt cccaattaag atacatctta tagtataccg ttattttttg agaaacccaa
    53341 tctgtttttt aatattaaga aaaacttgag aaataaagca gataagtaca cgaagagaaa
    53401 aaagaagaca aagccctcgt tcagactttg ccttttatga ttctctggta aaaattaaaa
    53461 tatccccatt atagaaacga gtcgcgacta tgtgttttcc cgttatcgat ataagacgta
    53521 tcattgatgc cgtggataga aaatcgaccg tttgcataag tgacctttgt tacgctgcca
    53581 tttaaaagcc cagcacgaac aggctgttta ggatcgatca gattaagcaa aaaggcgatc
    53641 gtcaaaccat gagatacgac taaaacattt ccaccaccat ttttttcgca gtgatgggcg
    53701 atatcttcaa atcctgtcca tacacggtct cgcaatgttt cataagtttc cgcccaccca
    53761 gcggtatcta agttgtatac agcattagca atttgttcaa aagaaacttc tgtggtaaac
    53821 atttcatcat atgttttaaa attcaataca cgagggatca caccccacat ttccatgtca
    53881 tatccacctt caagagaccc gaaacaccat tctctaatcc ttttttcaat aaaataagga
    53941 atttcttttc ccttagaatg ttctcctaga ataatacggg cagtttcgat tgcacgacca
    54001 ctgtcactag aataagcaaa tgaaaagtcg acatttttta acccgcgacc taaataatga
    54061 atgccttctt gtccttgctt tgtgaggggt gtatcacacc agccttgtac tcgttcaatt
    54121 gtattgaaca tcgtttttcc gtgtcgtacg atataaagtg tagtttctgt cattttattc
    54181 tcctcctaag tcgttttaac gattgaaaaa aggatttgtc cttttttcct gttcaatcgt
    54241 cgttgcatcg ccatgtcctg gatagactgc aaattcatca ggtaatacaa acagctgggt
    54301 ccgaatacta tgaagtaatt gttctaaatt ccctgtatac agatcggttc taccgatact
    54361 tcctctaaac agggcgtcgc cagaaacgac aaaatcatca aaaataaagc tgacgcttcc
    54421 tatagaatgc cctggggtag ggacgacgcg aaaagacatg tttcctaaag tataagtttt
    54481 catttcaaat tcatattctg caggcgagac aataacatca gcaatatcat catgtctacc
    54541 gagtccagaa agattcaaaa tcggatcacc tagccatttt tgctctagtg ggctgacata
    54601 aactgggatt tgataatacg cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt
    54661 atgggtaagc aaaatcgcta ccggctgttg gtttgttttt ttgatttctt gtttgattcg
    54721 ttccgcatct tctccagggt caatgatcaa aagtgcttct tcgttgtaga ccaaataaca
    54781 gttttcttca atcattcctg tttttaatct ttcgatatgc atgtgcattc ctccttgata
    54841 gccaaaactt ctttcatcca ttagtataac gcattcattg ctaggaaaca aaaacaggta
    54901 cttatttctc tttacttatc tgaattatca gactatatga aaagtatttg gatattctaa
    54961 agagaaggaa agtaagtttt catagcattt taatatgtcc ttgttacatt agattcatcc
    55021 aaaaatccaa attctatatc ctatatccct gcctaatcag cagggtgttt ttttatgttt
    55081 ggaaagagag ctctttttgc taaactaggt tttttcaggt aatcttagag agaaagaggt
    55141 ggtggaaatg atcaaacagt caaaaatatc tgtgcggcac ttagtagaat ttattttgcg
    55201 acgtggaagt atcgacaatc gtaaaaaaag taaccatact gcattagaag gagcgagaat
    55261 ccaccgcaag ctccaaaaag atgcgggaga aaactatgaa aaagaagtct ttttaaaaac
    55321 aacagttcaa ttagatgaat attacctgat cgttgaaggc cgtgcagacg gtattttcaa
    55381 aagagacggt atttattata tagatgaaat caaaacctcc gaaccacgat ttgaagattt
    55441 agaaccagaa caagtggaac ttttctttca tcaagcaagg gtgtatgcct atatctatag
    55501 tcaagaacat gatttacaag aaataaatct gcaattgact tacttccaga cggcagaaga
    55561 agtgatcacg agaaaggttg aacatcagac aagagagcag ttagaaactt tttttaaaaa
    55621 gctgactgaa gattataaag aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa
    55681 tgcctctttg atggctttga aattccccca tgatgagtat aggaaaggtc agcgtgaact
    55741 agcggtagct gcttataaaa cgatccgtac gaaacagaaa ttgtttgtag aagcacctac
    55801 cggaacagga aaaacaatct ctacgttatt ccccgctttg aaagctgtag gggaagaaga
    55861 gggagaaaag atcttttatc taacggctaa aacaataact agacaagtag ctgaagatgc
    55921 tatgacagca ttaaaagata caggagctga agtaaaaagt gtcactttga cggcgaaaga
    55981 caagatctgc tttttagatg agacgacctg taatccagat cagtgcccat atgccaatgg
    56041 ttattacaat cggattaatg aaggtttatg ggatcttttg aatcatgaga atcagatcac
    56101 tcgtgaagtc atcgaaactt atgcaagaaa gcatacactt tgtcctttcg aactatcatt
    56161 agatgtcagt atctggtgtg acgtaatcat tggggattac aattatttat ttgatccaac
    56221 tgtttattta agacgttttt ttgaagatga aaaaaacgag gactatctgt ttttgattga
    56281 cgaagcacat aatttggtga atcgttcccg agagatgtat tcggcagaac tatcttatga
    56341 aaaaacgaaa cgaagtaagg aagctatacc aaaggaattc aaaaaacttc atcgccgttt
    56401 caacaaacta ttaaaagaat ttgattcgat ccgagaaata gcaaaagaag atcattggga
    56461 ttatcatcat caaaaagcac cggctgaatc tttagtaaag gcaggttatc aattaagcga
    56521 aaaaattaaa gaatggctgg cagaatttcc tgagcaccca tcacaagaac aattgcttcc
    56581 ttattatttt gatttgcttc attttttgaa ggtcagtgaa ttttatgatg atcactacga
    56641 aacaacagtc gaaaaaacgt atcgcgatct gatagtaaaa gagttttgta ttgatccaag
    56701 cttgtttctt gagcagtcgt tggataaagg aaaaagcagt attctttttt ctgctagttt
    56761 ttcaccactc tcttattatc aggaaacact ggggggacaa aaaagtctgg cttacaaact
    56821 gccgagtcct ttcccagaag gaaaccagca agttttcatc gcaaattacc tagagacgac
    56881 ttatcgaaaa agggaacaca gtctggcaaa attagtagag acgattcatg cttttgctga
    56941 tggaaaggtc gggaattatt tcgttttctt tccttcttat caatatttag atcttgcggt
    57001 ggaagcattc agacagcaat atcccgacag caatgtgcta atccaagaaa cggacatgaa
    57061 tgaggaagaa cgcgaacgat ttttaacaaa atttcaaaca aatcccgctg aaacattgat
    57121 tggattttgc gtacttggcg ggattttttc agaaggaatc gatttgagag gggaccgatt
    57181 gatcggcagt atgatcgtag gtgtaggttt gccgcagatg aatcatgagc aggaactgat
    57241 caaatcatat tatgatgaga aggaacacct tggatttgct tacgcttatc agcttcctgg
    57301 aatgaacaaa gtcttgcaag ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat
    57361 cgttgtattg gcagatcaac gattttctag tttttcttat cgacgtttat tcccacaaca
    57421 ttggcaaaca gcaagagaag tacgaagtgt tggggaattg gaagaaggaa tacagcgttt
    57481 ttggttatca aaagaagcag taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt
    57541 ttttgattaa cgatagttag tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa
    57601 agcaatcaca gcttgtagat catcacggtt cttacctgtc actctaatct gatcttcttg
    57661 gatttgtgct tttactttca gtttttcatt tttgatagct gtagtgattt tcttcgcgtt
    57721 ttcacgatcg attccgttga ccaaatcggc agtttgtcga gctttgccac ctagagcgtg
    57781 ttctgattcg gaaaaatgga tatttttgat tggcacattt cttttgatca gcttaccaaa
    57841 taatacatct ttgatctgtt cgattttgaa atcatcatcg ctcaatacaa ctagattgct
    57901 gttctctaat ttgatttcta ctgtagaatc cttgaagtca aagcgattgg acaattcttt
    57961 tgttgtttgc tgaatcgcat tttttacttc ttctgtattg acttctgata caatgtcgaa
    58021 gctagcatct tttgccatag gaaccctcct gatatacata taaatattta ccatacatct
    58081 atcttatcat aggattaggg cagattctta cctgtttgct aaaattcttc tcatttgagt
    58141 tcgaagtatt ttattttaat agataaactg ttattcttac atttggaata ccaaagtagg
    58201 ggagaggtgc ttgtgagtaa aattatgact gcgtatcata aaaatgaaag attaaagaaa
    58261 atagtggtta ttttgataac cggattgacc gctgcggtcg ctttaaatta ttttttgatt
    58321 ccagccaatg tcttttctgc agggatgaat gggatcgctc aaatcatttc ctcgctttta
    58381 tatgaatggt ttcatatcaa gatagacaca ggaacattta tttttctttt gaatatccct
    58441 gtgtttgtat tagggtttct caaagtagga aaacgagcaa cgatccttag ttttgtgaat
    58501 gttgtatgtg tatcggtgat gacgatgctg cttccagtcg gacagatcac tgataatatt
    58561 ttgatgaatg ccctagtagg cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa
    58621 atgggcttta caactggtgg gatggatatc atctctcttg tactttcaca aacaactgga
    58681 aaaacagtcg gaaactacat gctgattcta aatggtatca ttgtagtcat cgcaggcttc
    58741 ttctttaact gggaaagtgc cttgtatacg attatttcca tttatgcaat gactcaagta
    58801 attgatgcca tccacacgag tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca
    58861 gaagaggtaa cagaagaact agcaaaacgg atggtacgtg gaatgacatt actgccgtct
    58921 gtcggtggct attcaaaaaa acaagggaac atgatcatga tggtcatcac ccgctacgaa
    58981 atgtatgatc ttgatcagat cgttcatgaa atcgacgacg atgcatttat caatatttta
    59041 ccaactcatt cagtatttgg ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca
    59101 actggcgtat tgccagaaat aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt
    59161 ttagaataaa tttaatctat tacatctaat aagaacattt ttataaattt aaacgagaat
    59221 cttacttcta aattctcatt tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt
    59281 gttaaggagt gtcgtacgat gagcgaagca aaccaagaac ggtctggaca agaaatagaa
    59341 ctaatcaaag aacgtattct tgctgccttg gaaatggtga ttgatcctga attaggaatc
    59401 gacatcgtca atttaggctt gatttatgat atagaattca atcctgaaaa tggagaaaca
    59461 gtcattaaga tgactctaac aactatgggc tgtccattag cagatatttt aacagaatct
    59521 atccatgacg cattaaaaga agtgccagaa gtaacgaaag cagaagtcaa actagtctgg
    59581 tatccagcat ggacgacaga taagatgagc cgttatgcac ggattgcgtt ggggatacga
    59641 tagaaatctt ggctgactag ctatgagaag caaataggaa aaacaaaaaa gccttttatc
    59701 ctttctagaa gaaaagagat aaaaggtttc tttgtttata aaattttgtc tgctatatta
    59761 taagttaatt aaataaatgt gttctattga taaaggacat tcaaatccct ttccttttgg
    59821 aatccttctt ttttacatca gtttgatttt cgtatagtat tctttctcat cttcacgata
    59881 aatcgcagcc ccattttttt gttgtgcttt aagactgcca agattttttt tatttacaga
    59941 caaaagtgcc tcatcaatgc cgattttttt tgcctcttgt agtactaatg ctaatccttg
    60001 tgtggcatat ccttttttac gataattagg ggagatgccg taaccaatat ggccagcgcc
    60061 gtttcttaat gtttcgttca gtttatggcg caaattaaaa attcctacat actcatgttc
    60121 atcactttct aaaataaaga cggttgcggg aacgtaactc tcaggaagat cgattcctaa
    60181 agacgttttt tcgcattttt ttacataatc taaaaattca gcaaatgtat agccataagc
    60241 attgttgata aatccactct catttggtcc aaagctagtt tgtagctgat aagctttctt
    60301 taaatctgct tcccatagtt tgatcagttt catcgtattc acctctttaa gtaaccttat
    60361 tcatttgttt tctactatag cactattgtt gtaaatagcc atgcctattt ttatttattg
    60421 tgtcatatcg tattaagaat catcgaacaa tacaaatatg aattacaata aaaaaattat
    60481 ttgaagttcg ttcattcgtt acttatgatc tctagaagaa agaaaacgtg aattacttca
    60541 gcgtctagaa ttccagaagg atagtaatca acagaagaaa taatcaatat ccaaatgaaa
    60601 gaagatagct gaatcgagaa aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta
    60661 ctaatcccag agtcgttcgt ttaacattga aatcgatcat atggataaaa cagatttttt
    60721 caccaaccct caatagatga tggagttgct cggatgaatt atttgtgtag atggcataag
    60781 ggagggcgta cagaaatgaa aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat
    60841 cctaaactaa aagatgtgat gcggctatgg cggaattggc agacgcgcta gcttcaggcg
    60901 ctagtgaggg tgacctcgtg gaagttcgag tcttcttagc cgcataattt gtttttttat
    60961 aaagagaagg tacacctgga gcgatgagaa agaaaataac atattatcta aaagaatata
    61021 ggaaaaattt taaacaacgg aaccagattc aaataaatca aaaaaaattt gtgtgaaggt
    61081 gggacagttt aaaacctaat agattgattc aaatagtaga tttatagatt ctgatacaag
    61141 aaatactggc aaacgatagt taaggaataa agactaaact taaactcaaa aagaggtcat
    61201 gcgaaagaat aaatcgcatg acctcttact atattttgaa tgtgtttaaa cgttcaaata
    61261 atgaatctta gtaccaaccg tttgcttccc agaatgcttt cgccgcttcc caagaaccat
    61321 aacgtgaagt aacatattgt tctgctactc tttcttggtt tgcagcagag tagtcaccat
    61381 ttaagtaaga agaatctaat tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag
    61441 aaccgcttga ttctttttgt gcgatccatt cttttgcaga gcttgtactt gctggagtag
    61501 cagcagtttc tgttacagca ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt
    61561 ccgtttgaac cgtttcttgt acaacaggtt gttcagcttg aacaggttca gcttggactg
    61621 gtgcttcttg aacggctgct tgaggttcag cagctgcttg tgcagagcct tctgttggga
    61681 ttgttaattg ttgtcctgag ta
//
LOCUS       DANJMI010000014        58319 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000014 DANJMI010000000
VERSION     DANJMI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.014"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            114..560
                     /gene="nrdI"
                     /locus_tag="PWU81_001029"
     CDS             114..560
                     /gene="nrdI"
                     /locus_tag="PWU81_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL1109754.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(625..2607)
                     /locus_tag="PWU81_001030"
     CDS             complement(625..2607)
                     /locus_tag="PWU81_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL1109755.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(2621..3517)
                     /gene="murQ"
                     /locus_tag="PWU81_001031"
     CDS             complement(2621..3517)
                     /gene="murQ"
                     /locus_tag="PWU81_001031"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL1109756.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(3520..4581)
                     /locus_tag="PWU81_001032"
     CDS             complement(3520..4581)
                     /locus_tag="PWU81_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL1109757.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(4792..5358)
                     /locus_tag="PWU81_001033"
     CDS             complement(4792..5358)
                     /locus_tag="PWU81_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL1109758.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(5386..6888)
                     /gene="eat(A)"
                     /locus_tag="PWU81_001034"
     CDS             complement(5386..6888)
                     /gene="eat(A)"
                     /locus_tag="PWU81_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL1109759.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(7146..8267)
                     /locus_tag="PWU81_001035"
     CDS             complement(7146..8267)
                     /locus_tag="PWU81_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL1109760.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            8393..9334
                     /locus_tag="PWU81_001036"
     CDS             8393..9334
                     /locus_tag="PWU81_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL1109761.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(9484..10380)
                     /locus_tag="PWU81_001037"
     CDS             complement(9484..10380)
                     /locus_tag="PWU81_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL1109762.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            10649..11989
                     /locus_tag="PWU81_001038"
     CDS             10649..11989
                     /locus_tag="PWU81_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1109763.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            12207..12650
                     /locus_tag="PWU81_001039"
     CDS             12207..12650
                     /locus_tag="PWU81_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDL1109764.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(13154..14572)
                     /gene="nhaC"
                     /locus_tag="PWU81_001040"
     CDS             complement(13154..14572)
                     /gene="nhaC"
                     /locus_tag="PWU81_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL1109765.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(14975..16198)
                     /locus_tag="PWU81_001041"
     CDS             complement(14975..16198)
                     /locus_tag="PWU81_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDL1109766.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(16229..17068)
                     /locus_tag="PWU81_001042"
     CDS             complement(16229..17068)
                     /locus_tag="PWU81_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL1109767.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(17085..17765)
                     /locus_tag="PWU81_001043"
     CDS             complement(17085..17765)
                     /locus_tag="PWU81_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1109768.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(17767..18840)
                     /locus_tag="PWU81_001044"
     CDS             complement(17767..18840)
                     /locus_tag="PWU81_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1109769.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(18896..19134)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(19177..19410)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(19487..20158)
                     /locus_tag="PWU81_001045"
     CDS             complement(19487..20158)
                     /locus_tag="PWU81_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL1109770.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(20245..22923)
                     /gene="mgtA"
                     /locus_tag="PWU81_001046"
     CDS             complement(20245..22923)
                     /gene="mgtA"
                     /locus_tag="PWU81_001046"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDL1109771.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            23582..24019
                     /gene="ndk"
                     /locus_tag="PWU81_001047"
     CDS             23582..24019
                     /gene="ndk"
                     /locus_tag="PWU81_001047"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL1109772.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(24041..24907)
                     /locus_tag="PWU81_001048"
     CDS             complement(24041..24907)
                     /locus_tag="PWU81_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL1109773.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(25067..27160)
                     /locus_tag="PWU81_001049"
     CDS             complement(25067..27160)
                     /locus_tag="PWU81_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL1109774.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(27909..28532)
                     /locus_tag="PWU81_001050"
     CDS             complement(27909..28532)
                     /locus_tag="PWU81_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109775.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(28691..30088)
                     /locus_tag="PWU81_001051"
     CDS             complement(28691..30088)
                     /locus_tag="PWU81_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL1109776.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(30120..31091)
                     /locus_tag="PWU81_001052"
     CDS             complement(30120..31091)
                     /locus_tag="PWU81_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1109777.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            31465..31890
                     /locus_tag="PWU81_001053"
     CDS             31465..31890
                     /locus_tag="PWU81_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109778.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(32378..33079)
                     /locus_tag="PWU81_001054"
     CDS             complement(32378..33079)
                     /locus_tag="PWU81_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL1109779.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(33095..33535)
                     /locus_tag="PWU81_001055"
     CDS             complement(33095..33535)
                     /locus_tag="PWU81_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL1109780.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(33535..34110)
                     /locus_tag="PWU81_001056"
     CDS             complement(33535..34110)
                     /locus_tag="PWU81_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDL1109781.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(34958..35437)
                     /locus_tag="PWU81_001057"
                     /pseudo
     CDS             complement(34958..35437)
                     /locus_tag="PWU81_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(35596..36297)
                     /locus_tag="PWU81_001058"
     CDS             complement(35596..36297)
                     /locus_tag="PWU81_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1109782.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(36405..37814)
                     /locus_tag="PWU81_001059"
     CDS             complement(36405..37814)
                     /locus_tag="PWU81_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDL1109783.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(37862..39727)
                     /locus_tag="PWU81_001060"
     CDS             complement(37862..39727)
                     /locus_tag="PWU81_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL1109784.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(39848..40698)
                     /locus_tag="PWU81_001061"
                     /pseudo
     CDS             complement(39848..40698)
                     /locus_tag="PWU81_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(41008..41721)
                     /locus_tag="PWU81_001062"
     CDS             complement(41008..41721)
                     /locus_tag="PWU81_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL1109785.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(41789..42115)
                     /locus_tag="PWU81_001063"
     CDS             complement(41789..42115)
                     /locus_tag="PWU81_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL1109786.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(42238..42465)
                     /locus_tag="PWU81_001064"
     CDS             complement(42238..42465)
                     /locus_tag="PWU81_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL1109787.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(42787..44040)
                     /locus_tag="PWU81_001065"
     CDS             complement(42787..44040)
                     /locus_tag="PWU81_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL1109788.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            44101..44841
                     /locus_tag="PWU81_001066"
     CDS             44101..44841
                     /locus_tag="PWU81_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1109789.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(44927..45685)
                     /locus_tag="PWU81_001067"
     CDS             complement(44927..45685)
                     /locus_tag="PWU81_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL1109790.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(45682..46710)
                     /locus_tag="PWU81_001068"
                     /pseudo
     CDS             complement(45682..46710)
                     /locus_tag="PWU81_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(46730..49897)
                     /locus_tag="PWU81_001069"
     CDS             complement(46730..49897)
                     /locus_tag="PWU81_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109791.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(50121..51005)
                     /locus_tag="PWU81_001070"
     CDS             complement(50121..51005)
                     /locus_tag="PWU81_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL1109792.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(51100..51537)
                     /locus_tag="PWU81_001071"
     CDS             complement(51100..51537)
                     /locus_tag="PWU81_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109793.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(51754..52665)
                     /locus_tag="PWU81_001072"
     CDS             complement(51754..52665)
                     /locus_tag="PWU81_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL1109794.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(53122..53550)
                     /locus_tag="PWU81_001073"
     CDS             complement(53122..53550)
                     /locus_tag="PWU81_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109795.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(53578..54216)
                     /locus_tag="PWU81_001074"
     CDS             complement(53578..54216)
                     /locus_tag="PWU81_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1109796.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(54220..56202)
                     /locus_tag="PWU81_001075"
     CDS             complement(54220..56202)
                     /locus_tag="PWU81_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDL1109797.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            56382..57047
                     /locus_tag="PWU81_001076"
     CDS             56382..57047
                     /locus_tag="PWU81_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1109798.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
BASE COUNT        16870 a        12141 c         9861 g        19447 t
ORIGIN      
        1 ctaccggctt ttttctcatt atacaagcaa agcgcctagt ccctttccaa attacaccac
       61 caaaaaactt aaaaaagatg ctatactaaa aggaaagaat tggaggatga tccatgaaca
      121 ttttatatat atcgatttca ggaaatacgc gtgcctttgc caaacatcta gcagaatatg
      181 ctgaaaaaat gcatgcagaa gatcctgtca atcctgaagt tactttaaaa gaaatccatg
      241 aaaatagtga ttttacgaaa gaaacagaac cattttttac tttcgttcct acttatttag
      301 acggcggaaa tggtttagat aacggtgata cagaaatctt gaccgaaacg atgcgtgaat
      361 atctggaaca tgaagacaat cataaacttt gtcttggtgt agtcggtagc ggaaataaaa
      421 acttcaataa tcagtattgt ctaacagcca aacaatatgc acaaacattt ggctttcctt
      481 tcttggcaga ttatgaatta cggggaacac catctgatgt gacacgaatt tatcagattt
      541 tagcagaaaa caataaataa taaaaaagaa gaagctgaaa aatagatttc agtttcttct
      601 ttcgtttcta ttgtttctat catattaagc tttgacatcc atcacaggtg cttttgcttt
      661 tgctttacct gttttttcca agacaaagtt ttttacacga tccatattgg ttaccaagac
      721 gatcatggat gtttctttgc ctgctgcttt gatcgcttct aagtcagctt ctgctacaag
      781 cgtatcaggt gtcacttgct gcccttctgt tactttcaca tcaaatggcg taccgcctaa
      841 gtcaactgtg ttgattccca tatgaagcaa gatttctaaa ccatttggcg ttgtgatccc
      901 aatagcgtgt ttcgttggga agacagagct gactacccca gtaactggag aatagatatg
      961 tccgttttct ggttctacaa agtagccttc acccatcatc ttttgtgcaa aaactggatc
     1021 acttgcttta tctaaagaaa caactgttcc gtcagcaact gatgagagga tagtcacact
     1081 ttcagctgaa gtttgaggta ctactttttc aggcacttca tttactgtgg ctgtttcagt
     1141 cgctaattct tctttttcta aacggtcttt aggaataccg aagaagaatg tagcaacgaa
     1201 gccaccagca taagctgcca ataatccaag gatgtagcct aaccatttac cgtcagagat
     1261 caatgggatc aaagcggctc ctgaaggtcc aatagcgata gccccgacat ttccgatcat
     1321 tccgatcact gccccgccga tacctccacc gatacaagca gtaataaatg ggcgtcctag
     1381 aggtaatgtt actccgtaaa tcaatggttc gccgattcct aggatcccga ctggtaaagc
     1441 tcctttgatc atttctgtta attctttatc ttttttgcag cgaatccata atgccaatgc
     1501 tgcacccact tgtcctgcac cagccatcgc aagaattgga agcaatagcg tacgaccagt
     1561 ttcatcaatc atttgcacat ggattggagt caaaatttga tgcagtccga acataaccat
     1621 cggcaagaat aatgtgccta gaacgaagcc ggcaaatgct ccaccaacgc caagtaccca
     1681 gttgatccca ccgaccatgc catcagaaat gaaacctgct aaaggcatga tgaagaaaat
     1741 ttcaatcaga ccgatggcaa tcaatgaaag agttggtgta acgatgatat ctaccgcatc
     1801 aggtactact ttatgcagtt ttttctcaac gattgacaat agccaaacag cgaagatgac
     1861 acctaggatc ccaccttgtc cagctgcaag agcttcacca gtaaataagt ttttcagtgg
     1921 tgcttccgga ttcatccctg tcagcatcac aacggccccg ataactccac caagagttgg
     1981 tgttgcacca aaaacttgtg cagcattgat acctgtatag ataactaggt agctaaacat
     2041 cccgtttttc aagatgttca tcacatcgat atattgttgc catgttgctg catcaagtgt
     2101 tccggctgtg actaagtttg ctaaaactgc ggcaatccct gccacgatcc cagtaccgac
     2161 aaatgcagga atcattggca caaagatatt agaaatgtct tttaataccg atttgatttt
     2221 agatggtttt tgtttcgatt tttgtgccgc tttcatttct tgtgcccgtt cgtttacacg
     2281 gtcttttcca gatgctgatg aatttgctgt agctgtactt acagaagaaa gtttttctcc
     2341 taatttcacg cctgccaaat caaccatttg ctgtgctact ttgttcactt tcccaggtcc
     2401 gatgatgact tgaagctgtt catcttctac tacaccaagc actcctggaa tcgcttttaa
     2461 ttcagagaca tttacaagat catcattgat gatcttcata cgcacacgag tcatgcagtg
     2521 gacaatgctt tctacattgc ccatgccgcc tgccccttta taaatttgtt ttgccagttc
     2581 ttccaaagtc agttcgtttt ttgccatggt cttttccccc tcataaggtc ccttttacaa
     2641 atccgtttgc ttcagctaat tttgcttgag cttcctcttt atcactattt gtcaaaatca
     2701 tgacgatagc cagcttaaca ttttgatctg cttcttcaaa gtacttttca gctgtttcgt
     2761 aatcagcttc tgtcgcttgc atgatgattc gttttgagcg ttcgattagt ttttcatttg
     2821 ttggtttgac atcgaccatc aagttattga atactttacc tataccaatc atactgatag
     2881 tagaaatcat attcaagata agtttttgtg ctgttcctga cttcaatcga gtagagcctg
     2941 tcaaaaattc tggacctgca tcgacttcga ttgggaaatc cgcgtgttta ctgatttcgg
     3001 cttgtttatt gcaagaaatc gttcctgtga cagcaccgat cgattttgcg taatctaatc
     3061 caccgatcac atatggtgta cgaccgctag ctgcgatacc aataactata tcattttttg
     3121 tcaaattcag cttgattaga tcttcttgcc ctagctcata agaatcttcg gccccttcga
     3181 cagctacagt cattgcttct tctccaccag caattagacc tatgaccatt tccggttcta
     3241 caccgaatgt cggtacgcat tcagcagcat ctaacacacc tagtcttcca cttgttccgg
     3301 ctcccatata aatcagacgt ccaccttttt taaaggactc aatcgtgtta gcaatcactg
     3361 gttcgatact tgtcagttct ttttcaaccg cagcggccac tttctgatct tctttgttca
     3421 ttagttgaac ggcttgagaa atactcatct catctaatcc aaaagtttct tggtttctgc
     3481 gttccgttgt taggttctct aaattgattt tgctcatttt tatcttcctt cttttcttat
     3541 aaattgatag ttcataccag ctttgatttg gttgatcaat ggtagctctt ctttgataac
     3601 tttagctact acattcactt tttcatccgc tggcaaatca tatttggtta attggatctc
     3661 acccatatag cgcaaatact tttcgttatc taaagtaata gaccctttca tacgcgtagc
     3721 tgtatttctt gctgggatag gcggaatctc tcgaaaacgt gcatctacgc ttcgtatgac
     3781 atctcttgca tcgtcttgcc gattcgtatg cttccctaat atatattcgt aaaattcttc
     3841 atctacaggt tccgtatgga gcaagactgt ctgttctttt tggaaagaag caaattgctc
     3901 ctgaacgttt tctgacagac ctgcatctcc gatgtaaaca atatccgtgt tacttgctaa
     3961 tagatctaga gcgcctgcaa gaggatggat ccctcgatgt tcttccaaag ttggcaatcc
     4021 ttgatatagt gggcctctca actctgtatc tcctggtaca aaaccttgga caaggagacc
     4081 ttgaattttg agccattgat tttttttctg ataccattct ttgtccagcc ctgtttcggg
     4141 acgtggataa taattatgcc aagcttcaat ctgtgagaaa tcagcttctg cttctttcaa
     4201 ttcatcgata tctttaggcg tgatcgtgct agcattcaaa ctgattttca attgatgtga
     4261 ccagtcggca atctgctgat tggaaatatg ataatccatt cttaaacctg ttacaccaat
     4321 tgctttcaaa ggcgttagat cttctaatga aaaacccgct cgttccaatg cctctcctga
     4381 aatatcgacc atcaattcta gtttattctt tttcgcttcg cttcctagtt cgatcaatcg
     4441 cttttgatat gccgaaacat catcttctgg tatatgcatg gaagtgaaaa taccgacaaa
     4501 gccattggat gccatttggc ggatatactg tttcttctct tctgacaaag aatcgttcat
     4561 aaatattgaa aagccaaaca tattatccct ctttttctgt atccgtcttc atttctatga
     4621 ctagagtata tatctaaaga aacaaaattt caatactttt attaaaataa ttaaatttca
     4681 tttctaaagg ttgagtttat attatcaaaa gaattgtcat aacctaaaaa aggcactgct
     4741 aagcaaaagc agtgccttga aatattttat ttaaataaca caaaaacata attactcata
     4801 agaaaccaat tctgtattat ctaccatgaa tgtaaagaac ccctcattag gcatcggatg
     4861 aaaataagca ttgatcactc ttatcaaggc attatggcaa actaacagat agacattttc
     4921 ttcgtcttcg atgcattctt tcaataaagg aacgatccgt gcatacacat ctaagactga
     4981 ctccccattt gggaatcgta cagcgaacgc taaacgtgct ttttgaaagt tctcatcttt
     5041 gcttggcagt ccatcataat ccccaaaatc catttctacc aatcgttcat caacggtcag
     5101 cggcaagctg agacgatcag caactgcttg tgctgtatct cttgctcgtt gaagagggga
     5161 atggatgatt ttcgtgattg gaacaggcaa atctactacc ttttctgcta gtttctgtgc
     5221 ctgcatctgt cctttttcag tcagcggcac atctgcccgt ccgcaaacta atccttgtgc
     5281 gttccaagtt gtttctccgt gtctcgttac ataaagcatg tgattctctc cttcgttggc
     5341 aaagttcccc ttaaccatac cttgttgttc ttgagattac aagggttaga ttctctcaag
     5401 agaaataatc tcagtagata ctttatccag gaaggtttga tcatgttcca ctagtaacat
     5461 ggcaggtttc acgttcaaga tcagttgttc taattgttct tgattgaaga catccaaata
     5521 attcaatggt tcatcccatg tatatagttc agctggctgt gataaagatt tagccaattc
     5581 cacttttttc cgttggccca tactcatctg ctcgatcttg ttatgaaaaa catctctttc
     5641 catcccaagc tttcggaggt tgttcaaaaa tgcttggtag tctacttggt ttttctctgc
     5701 aaattccgcc aacgttcctc gattgtcttc ataattttgg cttgcataac taatgctcag
     5761 atgttttggc tggtattttt ctcctataac cttgccgttg aatgccccca gaagataatg
     5821 gataatggat gactttcctg caccgttagg acctgaaatc gccacccgct gatgaggctc
     5881 gattgtaaaa tgaattggct cgaataacag attttcatac cctaattgaa gatcttctac
     5941 gcttaaaagt cgcttatggt gagacgcttg gctattcatc gtcaaagaat cgatatattc
     6001 gatatctttt aatagttttt ctttctcgct gatctgggtc tccatccgat tcacgattgc
     6061 tttggatcgt ttcatcgtcc gtgcagcatc agcacccact gctcctttat tcactcgtct
     6121 agattcagta tcgatgaatc cgacttgttt ctttgtttta tctccttctc gggaacgaga
     6181 ccattcggct ttttcagctg ctgttttctt tagcctactg acttcttttt tcaatttttc
     6241 attttgagcc atttcaaatt catcacgaag ttttttctgt tcttcataga tagagaaatt
     6301 cccttgataa agttccagtt gacttttttc gattgctaaa acatggtcca ctacttcatc
     6361 gataaatccc cggtcatggc tgaccacgat gaagccttgt tttttctttt tcaaataagc
     6421 cgctacttgt tttctaccag agatatccaa atgattcgtt ggttcatcga ttaacgggaa
     6481 atgagtgtca tccacaaata aaagtgccag taggactttt gtcttctctc cccccgatag
     6541 tgtgctgaat tctctccata agatttcagg atctgtttgc atcaattgga gctctctttc
     6601 gatttcccat atctcaaaat ccgtaatatc atttaacacg taataggtta aacgttcttt
     6661 atcttttgtc tgttgcggga aataggcaaa ttcttgctga tggattactt gcccttgata
     6721 aggtagtttg ttttgtagaa tattcagtaa agttgtcttt cctcgaccgt ttcgtccgat
     6781 aagtcctagt ttccattgtg tgtcaaaatt tagatttgct tgttcaaata ataatgtgcc
     6841 ttggctgtcg tagccgaatg tcagattttt tatttcgatt ttagacattt tccatcacct
     6901 cgtaaaaaaa gaccagtcct attttcagaa ctggctaaag aggatgcaag ggttggatac
     6961 acacaaaaag acccatacat cgaaaaaacc aagccaatcc cgaaaatctg cacactgctt
     7021 ccttgtctcc acaaggtaca gtctgaatgc agctttcatc aatcgactta atttttcaat
     7081 gcacggatcc ctcactttgt cctatatttg aaattacgat agcataaaga aaatgatttt
     7141 gtcaactact tcgggaggat ggtgaaaaat ctgctaagtg tctgtcctaa gatttttgtt
     7201 ttttctaatg tttgcctgtg ttccattgct tcttctaaac ctattggacc caaatgaata
     7261 cttaagacaa ttgggagaaa atcagaaagt ccttctagct cttggtctct acttccgcaa
     7321 atagcgatta ttggttttcg atattttttt gcaagcactg ctacaccgta tggaactttt
     7381 ccgtggatcg tttgctgatc gatccgccct tctcctgtaa tgatgacatc tgcttgttcg
     7441 atcttttttt ccaatccagt gatttggctg actaaatcga aaccagaaac catcttcgct
     7501 cccaacaaaa aaagggctcc accaataccg cctgctgctc cacttcctgg aatatcatct
     7561 aaatccacct cccagaaatc ttttatttgc tgagcaatct ttcctgcttg tttatctaag
     7621 aaagctagcg tcgtatctat tcctcctttt tgtcttccaa attgccaagc agctccttgt
     7681 tgacctgtat atggattggt aacatctgca gcgataatca attttacttt agaaacaaga
     7741 acgtttcttc gatcaaaatg aagtttttta gtagataata aaggatttcc ttctgtgtac
     7801 ccttctaaag aagcacccaa ggattggagt aatcctagac caccatcgct acacccgctt
     7861 cctcctaaag tgacaataat ttgttctact tcttgaagca gtgcatcttt aataagttct
     7921 cctaaggcat aactggaagc aagtcggata gtttgatccg ttggtttatc aagtagatct
     7981 aaccccaatg cttgtgcaga ttcgatgact gcggtgtttt ttccttgcca agttgtcaat
     8041 aaatagctga tttttcgctg acggaataag agatccatcc catcaatggt acgccatgtt
     8101 cccctgagcg cgtaatagat tgcttctgca gtattttctc cgccatctgc aatcggcaca
     8161 ttgatttttc ttcctttacg atcttcccag ccatttagaa ctgcctgatt tagttcaaat
     8221 gaagaagcac ttcctttaaa tgaatcaatt gccgccaaaa tatccatctg tatccctcac
     8281 tttcttctta agtaagacac tttttttcat ttttttcaaa caaaacaatg ctattttctt
     8341 ccatttttct atatactaaa agggaactca ttgaataaag gagagaaaaa acatgaaaat
     8401 cgcaatagct ggggccggag caatgggcag ccgaatcgga ttgatgctac atcaaagcgg
     8461 aaatgaggtc ctcttgatcg atcgttggcc tgctcacatt gaagccattc gtacaaatgg
     8521 gttgattgct gactttaacg gtaaagaagt ggtcgcaaaa ctgccaatct attcaccaga
     8581 agaaatcatc gaaagcaacg aacacgtgga cttgatcgta gcattaacaa aagccaatca
     8641 attagatgat atgttttgtt cgatccaatc cattattact gacaatactt atgttttgtg
     8701 tttactaaat ggattaggtc atgaagatgt actggaaaaa tatgtgccaa agaaaaatat
     8761 cttatttggg atcacgatgt ggacagctgg tctagctggt cccggaaaag tcacattgtt
     8821 aggtgatgga gaaatcgaac tagagaactt ggaaccagaa ggcgaagcat ttacaaaaaa
     8881 agttgtagaa gtctttcaag aagcaaattt aaatccgatc tatagccata acgttcgtta
     8941 ttccatttgg cgaaaagctt gtgtaaatgg aacattgaat ggtttatgca cgattttgga
     9001 ttgtaatatt gctgaactag gcgcgcaaaa agcggcagag agcatggttc gaacaatcgt
     9061 atctgaattt gcttctatcg cagcaaaaga aggaatcata ctagatcaag aagaagtgta
     9121 ccaacatatt gcttctactt atgatcctga caacatcggg ttgcattacc catctatgta
     9181 ccaagacctg atcaaaaacc atcgtttgac tgaaatcgac tacatcaatg gtgccatttg
     9241 gcgaaaagga caaaaatatg atattgctac accatattgt gcctttttga cccaattagt
     9301 ccatgcaaaa gaaggaatcc ttggcgcaga ataaaaggct ctctgatgag aatcagctga
     9361 atcagcagct ggttctcttt ttttaagccc aataaaagat caaaaagaaa acacaactct
     9421 catgatgcca taagaattgt gcttgtttgt ttcagtacat aatctcttca aaatgtaggt
     9481 tttttatttg ttttcaacag aggagaggtc atattcgtat tgctcataaa tagtggaatc
     9541 gttagtgata aatcgttttg cccaattttc tgcatcataa aattccaata gctctgatag
     9601 gattggctta aatggtgtat gcagaaattc cggtaattca tctaaatcac gttctcgaag
     9661 caaaaaaata ttttcaggac gataaaaatg ataatgagca atcgcttgat tatccgtcac
     9721 tagtttcttt tgaaaataca tggattcaaa tgtccgcaaa gtcactcctt gctgaccctc
     9781 ccgcaacagt tccagaatcc cgcgcgattc ccctgttttt ttcaggtact cttcataagt
     9841 aagatatcca ggttgttcat tccctcgatg tggaagaatg aaatagttgg tcgtaacatc
     9901 tctttttata aattcctgct tatagctttc tgctcgttct tttctcccat tattcgttgc
     9961 accaaaaaat agatccgtct ttggttcgct agaagaaaaa ttcatatgtt tcgaataaaa
    10021 tgaactatta tgtttcagcc caagttcttt tgcttccaaa aaatcaaaat gataaatctc
    10081 atcgattgcc ggttcatctt gtgcctgctt cagcatcgcc tcgtgttctg ctttgaagtg
    10141 attccaaaaa aataacacaa tttttgcatt ggtaaatggg cgcacgcgtc gagccatctc
    10201 catatcaaat ccataatcta aaaaaacgat cagctgataa tttgccaact gttgtctagc
    10261 tgatttaatg aattccgact gtagtaaatc ttttccggac caaacatcta aagagcggat
    10321 ggaaggcaaa aaataaagtg atttattttt tgcaactagc agtacgtcat ttttgttcat
    10381 cttttccgcc tcactttctc tgtcactttt tcctatgata gcctgtttaa atagtaaaaa
    10441 gccagtaaaa cgtttcctat atttgtatga aaatttaaca tttgtatgaa aatatgatga
    10501 aataaatcat tcttctatta aaattcgcct aaaagttttc tgaatagttg taattgtctg
    10561 aaaatttgtt atcattagtc attgttaaat attgctattt attcttaata taaattttga
    10621 ttgtgaattt aaaagaaaga ggtcatttat ggaagataaa aaattgaaaa aacaacttag
    10681 ctccagacat atcaccatgt tagctttagg tggagcaatc ggtgcaggtc tatttaaagg
    10741 aagtggcgaa gcgattggga tcgccggccc ttctgtattg attgcttttt tgattggtgg
    10801 gctgatttta tatatcgtga tgaaaggtct gggaaaaatc gttttaagcg gtggcgacac
    10861 gcatcacgga ttatctggtt tgatacgccc ttatctaggt gctcattctg ctgattttac
    10921 tgattgggtc tactggtcta tgtggattat caatatcatc gctgaagctg ttgcagctgc
    10981 ttcctttttg cagttgtggt tcccgcacat tccagcctgg gtatttgttt ttatgttggc
    11041 tgttttaact acgatcatca atctttattc ggtacggtta ttcgcagaaa cagaatattg
    11101 gctggctttt gctaaaatca gtgtcattat cttactgatc atttttgcta cttatcttgt
    11161 cggtcagcaa atgttgggca caggcgtttt ccctaccctt cagcaattga ctgaccatgg
    11221 tgggtttact cctcatggta tgaagggaat catcagttct ttgcttgtag ttatctattc
    11281 ttacggcggt tctgaactga ttgctatcac ggtaagcgaa gcagatgatc ctaaacgtgc
    11341 gattcctaaa gcaatcaaag gtgtcatggg aagaatcatt tccttttaca tcattccttt
    11401 gtttttgctt ttgattcttt ttccttggaa tactttagca ggcacgaatg tcagtccttt
    11461 tgtgatggta tttgaaaaaa tgaatatccc atttgcagcg gatatcgtca attttgtgat
    11521 tgtccttgct ttgttctcat ctatcaattc gggtgtgtat gcatcttcaa gaattttgtt
    11581 cttccgtttg aaggatcgaa aaggttcctc tagaaaacta gctgttttaa acaagcatca
    11641 agtaccgcaa cgtgctgttt tattttgtac cagtgttctt tatcttggtg tagctctttc
    11701 ttattttgtc ggtgacaaat tatttggcta tctggcaggt tccctttcgt atacagtatt
    11761 attgatctgg atcattatca gtgcggcatc ctttgtactg gcactaagaa aaggaacatt
    11821 atgggataaa ggaatcagct tttttgcatt agctgtctta ggattgattt tccttggtat
    11881 cctgttcacg aactccattg gggtaactgt attgactggc ctgctttacc tgttcattta
    11941 tttcagttat caaaaaaaga atgatgcgtt cgtattgacg aatgaatgaa atcgatagaa
    12001 aattttattt tatttatgtg gaatagatgg agctattgaa gaaaggagtt gtgacaaaag
    12061 tcttgttgca actccttttt ccgaataaac agtctcatga cctcttggta aacggtgttc
    12121 aaaaaccctc tgtgcaaaat aattgattgt tctccatgct catgacatga tagaagaaag
    12181 gaatgaaaaa aaggagtttg ttttaaatga aaaagtattg gaagtttttg ttgatgatcg
    12241 gtgtttcaac ggtagttatg tttggtatga tgtacttgaa tgtctatgcg ctcgatcata
    12301 tcttcttcag tcgtacacga gtattcatgg cgttgatgat gggtgcagtc atggcagtca
    12361 tcatgctgtt gttcatgtgg aaaatgtatg aaaacaaaaa gataaatgtt atgatcttag
    12421 ccgctagtct tcttgttttt gccggttcct tgtttatggt acgaagccaa actactgtca
    12481 acgacacagc ttggatgaag gccatgattc ctcatcattc tatcgctatc ctggcaagta
    12541 aacgagctga tttaaaagat cctgaagtaa aaaaattagc ggaagaaatc attcaggcac
    12601 aagaagaaga gatcgacaaa atgaaaaaaa tgatcaaaga aatggaatga ccattatccg
    12661 tccaaataaa aaaagaggat gttccagaag ttatcagctt caagaaataa gaagaagcta
    12721 cttctggaac gccgtgaata aggggttgtg aaaaagctgc cctgcatcaa gtaataagaa
    12781 cagccaaaca aaaatagctt ctcctatttt tcgtttggtt aggcttatta ccgcagatgc
    12841 aagcttttga acaccgttta ttaagaggtt gtaaaatcag cgttctgacc tgagtattag
    12901 agagacaaat ggaaaaatag ttcctcatat tttgccattt tttgagctaa taccgagggt
    12961 cagctgattg aacaccgttt atcgagaggt tgtgaaaaag tcgcttagtt tcgcgtatta
    13021 agaaaaattt tgtgaaatgg ctttccacat ttttgtataa atttggctta ataccgagaa
    13081 actggctttt gaataccgtt tattcgtaaa agggattgtg acaaaagtct tgtcacaatc
    13141 cctttttatg tttttattgt tctaattgcg ctgtcttgct tactgcttct tgcggtttca
    13201 caaatttttt tgagattccc caacctgtga aggcactgac catcgaaaac acaggagaaa
    13261 gcaaactgaa aaatacgaat ggcaagtaag tgagtgtagg aataccaaat gtacttgcta
    13321 cgaaacttcc ggcaattccc caaggcacta agtagttgat tactgttcca ccatcttcta
    13381 gcgttctacc taatacagtt ggatcaaatc ctgctttttc ataagtttct ttaaatgcat
    13441 taccaggcag aataacagat aggaattgtt caccaataaa aatatttact ccgatacttg
    13501 tcatcaatgt tgtaaaaatc aaatttggtg tgttcgtcat ctttcttgat actcgttcca
    13561 tcacggttga gatcaagcca aattcgatca acaatccacc taatgacaaa gttagaataa
    13621 tcaatgacac agtaggcatc atactttcga ttcctccgcg gctaagcagt gaatcgattt
    13681 gttggtttcc agtatttgaa acaaaccctg attcgatgac cccagccaac ttcgttaagg
    13741 aagtttgtgg gttttgaata aaaatcattc ctactgctac agcgatattc aacaaaatcg
    13801 ttaaaattgc cggcaccttt ttccatgcac acagtaacat taaaaataaa ggaatcagtg
    13861 accagattga aatagtaaag tttgcttcta gaacatcagt cacttcacga atagatgaaa
    13921 gacttgcggc tttacttgtg tgtcctaaaa tcatgaataa aaagaaagaa acaacaaatg
    13981 ccgggacagt tgaccacatc atatgcttga tgtgtttgaa caaatcggca tcaacaatag
    14041 ctgctgctag atttgttgat tcggataatg gagacatctt atctccaaaa acggcacctg
    14101 aaacaatagc tccaacaacc agcgctggat ttaagcctag tgttgtccca ataccgaaaa
    14161 atgcaatccc aatcgttgac ataacagtaa aggcactccc tacagcgcta cctacgattg
    14221 cacacacaac aaatacagaa gggacaaacc actggacact gatcaatttg aacccaatga
    14281 ccattagagt tggaatgatc cctgcttgta tccatacagc aatcaatgaa ccgactaaaa
    14341 taaaaataaa gattgggatg attcctggtt tgatgccatt gatgattcct tcgtgcagcc
    14401 aatctgtcgg gtatcttttt atcactgcat acaacatgac cattccgatc gctgctaaga
    14461 tcgtgatatt aggtgagacc tttaatccga caacgccaat cgcaatgcct aaaacaataa
    14521 ccattaatac aagtgttgct tctaaaaaac ttggtgttcc tttttttccc atgataatct
    14581 ctcctttttt agttgttctt atgttctaaa aaagttaaga tatcggatta ctccgccgca
    14641 attcatccgc gattcctcct ttataaaaat aaaaagacct gttttaaact aaggacgaat
    14701 gactccgcgg taccacctta attaaaaaca gatctgtttt tcactttcaa acttttattc
    14761 aaatgtatgg actttccatg taattcgtca attaggcttg atcggttcac agcgaccacc
    14821 gactttctga acaaaacacc taaccgctac tgcgtgaaaa gttgacttgt acgaagttta
    14881 ccttccataa gatcatttgt caatgaaaaa gaaaatattt tttcattttt ctgaaaatta
    14941 tttttataac tttcacacac aagtttattt tttactagaa agtagcgccc tcttgttttt
    15001 ttcttgctac tatttcttga tgttcttgct tgattctttc taatagcttt ggctctttca
    15061 acagatcaag agctgtattg gcgagtagtt ctgctgcaat cgcaatagaa gcaaggcctt
    15121 tgtcactttt agcagcagct ttgaattctt ctgtgtgtcc ggcaatatat gtctcagaga
    15181 ttgctaccgt aggctggatt gttggtatga cttggctgac atttcctaca tcagaagaac
    15241 cgagacttgt tctttgttcc gtttctattt cttcatctgg aacacctgct tcttttgcat
    15301 gctggaagaa taagtcatca aaagaaggag taacgatgac atcatcaact gcattttgga
    15361 acaatccaaa ctcaaaagtc gtgcccgtac ttatcgcagc acctcgtacg atattttcca
    15421 ctttttcata tactttatct aatgtttgcc gatttcctgc acgtaaatag aaacgtccag
    15481 cagcgtaatc tggaatgacg ttggcagcta ctcctccgtc tgtaatgatc ccatggatat
    15541 tgacatcttt tggcaattgc aagcgtagag cgttgattcc attaaacact tggatcaatg
    15601 cttccaaagc atttactcct ttttcaggtg ctgctgctgc atgggaagcg acaccgtaaa
    15661 atttgatgtc gactggatca ttcgctaaag attctgtcgt tttcccataa cgaaaagctg
    15721 gatggacgca taacgctaca tcgacatcct caaaaaaacc ttctcgaaca aagctgcctt
    15781 tagctgatcc gttctcgccc ccttcttccc ctggggtgcc atatacacgg atttctccac
    15841 cagtttcatc aatgaattgc ttcactatgc tggcggctaa tacggagtaa gtaccaaaca
    15901 aattatgccc gcatgcatga ccaatacctg gtaaagcatc gaattctgcc aaaaaggcaa
    15961 ttgtgggacc gggtttattg gaaatataac gtgcatcaaa ggctgttcgg tgtccagcta
    16021 cttctttttc tacccaaaac ctttcttttt ctaattgttt aatcaacgct gctgaggaat
    16081 aaaactcata gttacttact tcaggatgtt cgtggatatc tagagcaagt gcttgatatt
    16141 ctggtaaacg atattggatt agatccttca attggtttgt tgagattgtt gttgtcataa
    16201 aaaattcctc ctatgctagt catttttctt attttctccc gaatgtttcc catgcaggca
    16261 cgcttgatcc ttttgatgtt ttctcgatta ctttgactgt gtcttctgtt tgataagcgt
    16321 ctactatttt ttgatacgtt ttattgtttt catcttcttt tcttgcaacg atgatattca
    16381 cataaggacg tgcatggtca tccactggtt ctaagaagat ggcatcagaa gcaggattaa
    16441 atccagcatc tacagctaca ccgctattga tcactgaaat gtctacatct gacaaagcac
    16501 gtgctgtttg ggaagcatct aattcagaaa tgtctagatt ttttctattc tcggtaatat
    16561 cagaaacagt gggcgcttgt ccttttgcct tatccacttt gatcaatcca gcagtttgca
    16621 ataacagaag agctctgccg ccatttgtaa catcattagg gattgctact tttgctccgt
    16681 ctttgatatc agctacatcc ttcaaagtgt tagaataaat tcccagcggt gcattgacag
    16741 tatttccgat cgatactaaa tcagttccgt gttctttatt gtaattgtct aaaaagaatt
    16801 gatgttggaa agaattcaaa tcaatcgatc catctgctaa agccacattt ggttgattgt
    16861 aatctgtaaa tttcacgtat tccaaattaa tgttctcctt tttcaaacgg tctttcacgt
    16921 tgtcccatac atctgtatct tctccaataa tccctagttt tacgatagtt tcttttttgt
    16981 cagttgtatc tgcttttcct tggctacacc ctgccaacca aataattgct acaaatgtaa
    17041 tgactacaaa acgaataatt tttttcattt tttctcctcc actttcaatg tgtaattttt
    17101 ttaatcagtt gattactgat aaattgactt aaaaaaacta agattagaat caataatgta
    17161 gccacaatcg ttacatctgt ttggaatcga ttataccctc tcgatatagc aagattccct
    17221 aatccgcctg cacctactgc tccagccatg gtagtcagtc cgatcacgtt gatgatagtc
    17281 aacgccgaga cccgaatgat accaggcaaa ccttctaaca aatacacacg taaaatgata
    17341 ccgattggac tggttcccat cgcttctgcc gcttcgatca ccccaggatc aacttctagc
    17401 agtgcgttct cgatttgacg agcaaaaaac ggaacaactc caaaaatcaa tggcactaaa
    17461 gctgctttcg caccaatcgt cgttccaact aagaaacgag tagtcaatgc taaaagagaa
    17521 agcaagataa tgaaagggat ggaacgaatg atattgatca gcttatcaag tatttcaaat
    17581 agcagacggt tagctagtaa tccattaggg cgtgtcacta ctaacgtcac acctaagatg
    17641 attccaatca tcccgccaat gaatgctgtc caaaaagaca tatatagtgt ttgcttcgtc
    17701 gcttcgacaa attcatccgg aatcaaataa acattgggca aatatgtttt aaagaaatca
    17761 gtcattttat cttcctcctt cgatcacttt gatacgacta tctaaatcgt ttttttgtaa
    17821 aatatttatt tttacaccag atttgatcaa ataatccaat gcttttttcc gctgtttggt
    17881 ttctccagct aaagtaacga tcaaactccc taacggcgtt tcttgtaata actctacatt
    17941 gccatataaa atattcgctg ttacttgata tttgctgtac agatgagcga ttagaggttc
    18001 gttcgtctga tttccaatat aacttaattc caccagccat ttattggcga tctgatcggc
    18061 aaaagcagaa tgggcaatga tcgtttctaa tgcttggtca agatgagtag ctgttcgaat
    18121 aaaatctttg gtcaattctt cttcaggatt gctgaagatc tggatgctgc tacctttttc
    18181 aacgattctt ccatcttcca taaccgctac cttgttgcag atttctttga caacttgcat
    18241 ctcatgagtg atcaggacga ttgttaaacc taactggcga ttcaattttt ttaacaatgc
    18301 aaggatctga agggtcgttt tcggatcaag tgcactggtt gcttcatcac ataataatac
    18361 cttaggttca ttcgccaaag ctcgtgcgat tgctactcgt tgtttttgtc cccccgacag
    18421 ttgtttagga taagcagatg ctttttcttc caaaccgaca agttccagta gttcattcac
    18481 tttttgacga cgttcctgtt tcgattttcc agaatacttt aaagaaaaat caacgttgtc
    18541 aaaaatagtt cggctttcca ttagattgaa atgctgaaag atcataccga tggcctttct
    18601 ttcttttcgc aattctttgg gagagagctg actcagattc gtctgattga caatgacttc
    18661 tcctgctgtt ggtttctgca gcaaattcat cacacgaact aacgtacttt tccctgcacc
    18721 tgagtaaccg acgattccat aaacatcgcc tttttcaatc gttaaatcta cagaatctac
    18781 tgcctgtatc tctttttctt tttgtttgaa acatacagaa acatctcgca attcaatcat
    18841 taaaattcct ccttatctaa aaaaagcgtc ctcaagaaaa tgaaaatgtc attttcttga
    18901 ggacgctttt ttgcgtgtta ccacctcatg ttcaagctgt gttcacacac aactcctctt
    18961 caagtacgat catactctag caagatatcg gtcgcagccg tcatcgcctt acatctatgc
    19021 tcgggatgaa ttgctcgatg gccattttcc aatagtcgtt ccttgcttct tctcagctac
    19081 cgaagctctc tgtcaaaggt tcttctatcg tacttaccat ttcaatgcaa ataatattca
    19141 aaaaaaaagt ccttttacta acataaacta tgtcagtaaa aggacgacgt atcgcggtac
    19201 cacctttttt cggaagtttc cttccctcaa gcccaacatc tcaaaaagag agattggcgt
    19261 tcgttttaac ggtcgaacag ttccgaattt gcctacttat cgacaaatta ttctccaagg
    19321 ccattttcag cttttcttac ttgactcttc tcaccagcca gagcctctct gcaaagtttc
    19381 caaacttact ttccttttca acgaaatcga tattttgttg ttgggattaa tctacatgag
    19441 gaaaactgaa aagtcaagta cgaaattcaa attatttttt ctatatttat ttttctgttt
    19501 ttttatttaa catttcaata acagttcgaa gatcttttcc ttccgcaaga agtttcaatt
    19561 tttccttttc ttcgacggta taatttcgga caaaactgga atcaggtatc aaaacaacgc
    19621 ctaatcctgc tcgtgaagca gctgttgctc ctactaaaga atcttccact gcaaaggctg
    19681 aactagcttc taatcctgtt cgttccaaag ctgtcagata aggaatcggt gaaggcttgt
    19741 tctccgacac ttcgtctccg aatgtaaacg tgtccaagta atcagctaat ttaaagtgat
    19801 ccaagatatc tgttgctctt ttcgtaacag tggtcgtagc aatgcctagt ttcatatgac
    19861 gttcttttaa gtctgccaat atttcttttg catatggttt taagtggatg gctccgcttg
    19921 tcagctgttg ataaaaatat tcttcacgct tcgctcggac ttcctgtact ttttcagggc
    19981 tacctgctgc tttcactaaa atatcaaaac tttgtttcca gttttttccg ccccaagcgt
    20041 caacgatttc ttttgggata tggatatcaa attgctgaaa tccccaaagc cagccatcac
    20101 gatacgtttt ttctgtgtca actaacaaac catctaaatc aaatattgca cctttcatct
    20161 atacattaat cctttccaaa acaatacaca tagtttagca cgactggtcc aaaaagaaca
    20221 gaagcaaaga accagccttc tcgtttataa gagttcttgg aatcgacgga tatagatttt
    20281 cttcacagct gtcacaagcg ccagataagc gatgactgta gcaaataacc ataaccagta
    20341 attgccagga agtggcatca agcctaattc agcaccaaaa cttgtaaaag gaaggacaga
    20401 accgacaata attcctaatg tagtgataac aaacatggca aatgttgcgt tgctttggat
    20461 aaatggtact ttcggtgtac gcagtgcatg gagcactaag gtttgtgtcc atagtgattc
    20521 gacaaaccat cctgcatgga ataatgcgat gaaaatcact cgctgtgaac tatccaatgt
    20581 atggaaactt ccgcccacta cagcaggaca aataacaaaa tacatcaaca agtaagtagt
    20641 aatatcaaat atcgaacttg ttggaccaaa gtaaaccatg aattccccaa tactagatgc
    20701 attccatttt tttggctcgt ccaaatattc tttatccatc cgatcccatg gcaaggaaat
    20761 gcaggaaaca tcatagatca gattcaaaaa caaaagttgt aaaggcagca ttggtaaaaa
    20821 tggcaggaac gtacttgcaa tcagcacgga aaacatattg ccaaaattag aacttgctgt
    20881 agccttgata tatttcatga tattcccaaa agtttctcgt ccagaaagaa ggcctctttc
    20941 caaaatcgtc aaatcttttt ctaaaaggat cacgtctgca gattctttcg caatatccac
    21001 agctgtatct acagaaatac cgatatcagc cactttcatt gcaggagcgt cattgatacc
    21061 atctcctaaa aatccgacag tatgaccatt tttacgtaaa gctgtcgtca aacgtgcttt
    21121 ttgctgagga ttcaatttta cgaatacttc atggtcttca gctacttgtg ctaattcatg
    21181 atcactcatt tccgagattt tctcaccggt aaccaattca ttgatctcta gacctacttg
    21241 acggcacact gaacgtgtaa ctaattcgtt gtccccagtc agaactttta cagctacacc
    21301 atgttctttc agagctttca acgcttgttt cgttgtttct tttggtggat caaggaaagc
    21361 aagataacca atcaaaacca tgtctgactc atctttgact gaaaattctc caacgacgct
    21421 tggattggtc ttttgtgcga caccaatcac tcgtaagcca tcttcattaa gtgactgtac
    21481 ttttgcaagt acggcttctt ttcgttcttt tgttaatggt gtaacagtac cgccaagatc
    21541 aataaattta gaaatgttca gcatttcttc aatcgcgcct tttgtgatca tttgtgtttt
    21601 accagctgga tcagcaacca caacactcat acgacgacgt tcaaaatcaa aaggaatttc
    21661 atccactttt tgataattga tcttatctgt atctaaagtt tctgttgctt catcaatgat
    21721 agccacatct aacaaatttt tcagccctgt ttgatagtag ctatttagat acgcatggcg
    21781 caaaacacga tcatcttcat ttccttcaca atccatatga tattcaagaa tgattttatc
    21841 ttgtgtcaat gtccccgttt tatccgtaca taacacatcg attgcaccaa agttctggat
    21901 ggcattcaaa tttttgatca ctgttccttt tttggccatc acacttgctc ctttgaccaa
    21961 attcgtcgta acgatcattg gaagcatttc aggtgttaga ccaactgcta cagatagtcc
    22021 aaaaagaaaa gcttctagcc aatccccttt tgtaagccca ttgatgacga taactgctgg
    22081 agccatcaat gacataaatt tgatcagtag catggatgtt ttatggatgc ctaattcaaa
    22141 gcttgattga attggttttt cagatagcgt ttctgctaca tgaccaaata gtgtttcatt
    22201 tccaacagaa acaatgattc cttccgcact tccactgatg acattacttc ccataaagac
    22261 aagattttcg taacttgttt cgctttgctg atcgtcttca catactttcg catgcttttc
    22321 tactggatag ctttcccctg tcaatgctgc ttgggaaatg aataaatcct ttgcggacaa
    22381 taaacgcata tccgctggta tcatatcccc tgctgaaagc cgaacaagat ctccacagac
    22441 gatttcttcc ataggaatct ctgtgtactt tccattacgt tttacagctg ccgttacttt
    22501 cacaagtgat ttcagttttt cagccgcttg attggaacgt acggactgaa tcaacgtcat
    22561 tgtcccgctg acgataacca tagtgaagat aatggctaca ccgaaaagat ctttatctcc
    22621 aggagctgct aatacatatt ccgtgatgaa tgaaataatt cctaatgcga tcaacactaa
    22681 tgtaaatggc gtgatgtatg ctttcaatat ttctacaatg aatggtgttt ttttgccata
    22741 tgaaatcgca ttttcaccgt aattttctct tgaacgttcg atttgttctt ctgtcaatcc
    22801 atttgctgat gcatggaata gttcaaataa ttcttgtttc gtttttccag caaaagctgc
    22861 atagattttt tgtgtatctt tctttttttc catctcaatc tctcctttgt gtccaaaaga
    22921 cagctgttct cgctgctttt ggtctttagc tattcatcgg atcgatagag aaaaaactga
    22981 aaggggctga gaacagatag ataatagccc tggcctttcc tctagatcct tgtttttcga
    23041 atgactgtca tccatttgtt ctcatctcct ttactaagta attttaattc atgacttcca
    23101 atcatttttc gaatagttat ttttccaatt agaccacctc ctttttattt ttcaccgatg
    23161 gggatttgcg gcaacaaaaa aacctctcgc caaatactca gcgaaaggtt tgaaaaagca
    23221 tattaaaaaa agcttctcag atctcatcgt tgagttttga cactgtacaa cgtaaaaagc
    23281 agatacctgc ttttagctac cttatgaaaa gccttatctc gacaattcct gttctaccca
    23341 ttggcgtctc tcgacgtttt tgggcagtag cctatgtttg tataggagcc tcacctaaca
    23401 aatcttattc ggttttcctt cgttaatata acaactaaaa aagaggatgt caaatcaatt
    23461 ttccatgttt ttcatacttt ttttaaaata cacacgttat attcctgtct cacgcggttt
    23521 tatttgtctc attttccacc tcccgctata atagaaatat agtaggaaac gaggtgctgt
    23581 tatggaaaga acacttgtta ttatcaagcc agacggcgtg agacgccatt tagtcggaag
    23641 catcatccaa cgttttgaag caaagggact ggccattgct gaaatgaagt ttgaaactat
    23701 gactcctgaa ttagcaaaag aacattatca gcacttgact gagcgcagtt tttttgatga
    23761 actgatcgat tacatgactt ccggtcctgt cgtttatctt gtgctggtcg gcgaggaagt
    23821 gatcgatatc gtccgtaaga tggttggagc aacaaaagct gccgatgctg ttccaggtac
    23881 gatccgagga gactacgcgc tgccggggac tgaaaacatc attcacgctt ctgattcccg
    23941 tgatgctgcg gtaaaagaga tcgcccgctt tttcccgcca ttcgatataa acaaaccgtc
    24001 tgaaaaagcg gcgaagtgat acaaattcta aactgctggt ctagttgctg ggttcagcaa
    24061 tggccagcag ttttttctct aaggacctcc gttgattctt tcctaacata tattcgagca
    24121 ctgtgaagaa ttcttggtag tctttcgtct tttcttcccc taaagcagca gaaatcaatc
    24181 tttgataata atctgccatc aatgtttcat agatcactgt acttctttct ttatgtgata
    24241 agctccgttt catatcttca ttcacactac gtgccttttt caaccgacca ttttctatat
    24301 aaaacgaagt caggttcatt aaaaaagtaa agagatactg ctcatgattg ttcaactgtg
    24361 ctaatcgtcg cattcttttt ttgattttca aataattgtt ctcaatgaac cgatcactga
    24421 atatccaagg aaaatcagct agaatagata gttcaaattg accatagtct ggtatctgca
    24481 tcaagtattc ttcaatgatt cggcattcat aacgtaaaaa ttcaaattct gtctcagata
    24541 cttctgtttt ttcgaccatg tgaagattca aaaccaaata atacagatct tctgttttct
    24601 taaaatgcct acggttattg ctgatctctt tataaggatc aaagctgtct tcttgtaccc
    24661 gatctagcat tcgtcttttt ttctttaact ttattggttc atccagctgg tttcgcatcc
    24721 attcatattc tgcaagtgtc atgttcattc gctctaaaaa tgatctcaat gttacaaatg
    24781 acattttttt cactgaattt gtctcaatcc cgactagaga agtcttaaaa cagattcctt
    24841 ccgctaattg ggactgtgtc aacttcttgt tgactcgaag tttcttgatc aattcaccat
    24901 catacataat agttctcctt tttaatttaa atttttccac attcaataaa tagatacgtt
    24961 aaaaaggaaa aatacacgaa aaaaataaaa aaatgttttt ttcataaaaa ataccaacct
    25021 tcaagcatta gaatgttgtc taactactga aggttggttt gattgtttat tgattatttt
    25081 tttgctgttc aacgtctgtt cttgttactg gatataagtt ggttgttgtt ttattaccat
    25141 tttttgaaaa gacacttgtc gaatgttcgc ctttttgctt ttcgattgct tcattacgtt
    25201 ccgcttcatt tttatagtca tattttttcg gatcaatcgt ctcaaatccg tttggcacat
    25261 aaaaccgcaa gagattttcc tgattcagct tatctgaaag acttaacgca gtcttgactt
    25321 tttcctgatc tttggagatt tcctcatcga ttcctgctgc tttcgtatcg atgatttctc
    25381 ctgtctgatt attgtaagct tttcctccta ggacggtgta tttgggtgtc acaaagttcc
    25441 catttcgaaa agcaactact tgatcatgct gagaagataa taagtctgta ccaaaatgaa
    25501 gataatcttt atcatcgatt cccaatagat gtagtaatgt cggcaaaaca tcgatttctc
    25561 caccgtattc atgattgact gtccctttag tataaccagg catatggatc atcaatggca
    25621 ctcgttgcat ctgtgcgttg tcaaattcat cccatgtatc tggatcttta ccaagagcgc
    25681 ttgctaaatc tttattatct gtattcgaga tcccaaaatg atccccgtaa atcacaaaga
    25741 ttgaattttg gtaaatacca gattttttca aatacgtaaa aaattgttcc aatgattgat
    25801 ccagataatg tgccgttcga acataattat ctactgtgct attgccagta ttcaaagatg
    25861 gaaagtcgaa attcttgtca tccgtataat aaggcgtgtg gttggttacg gaaagaaatt
    25921 tcgtgtaaaa cggctgttgt agatgctcaa gatattgggc agattctctg aataaatctt
    25981 tatctaatat cccataacct aacgtttcgt cggattcatc aaaataagat cgatcaaaga
    26041 aattgtcata acctaagttt ttatatacat gatctctatt ccaaaaacta gctacattcc
    26101 catggaaaac agcgcttgta tagccttgct tttgtccaag gatcgctggt gcggcttgaa
    26161 agacattgtc tgacccaagt tctgtgaata atgagccttg aggtaaacca aatgtgccag
    26221 tttccaacat attttcagca tcacttgtct ttccctgtcc tacttcatga aagaaattgt
    26281 caaagctgat cgttgcttga ttctggaaaa tactatttaa aaacggtgtg acttcttgtc
    26341 cgtctacctt catattaatc aaaaactgct ggaaactttc taaatggaga acgatcacat
    26401 ttttcccttt agcgatccca aatgtttcgg gatttggctc tgcataatgt ttctttgtat
    26461 aatctaaaac ttcatcaatc ccattactgc ttgcatgcgc gcgaacactg ctagtcattc
    26521 ctgttttgat tccgtcataa atagtatacg catcgattcc tagatatttg acaatatatg
    26581 aacggtcaaa tgttcgctgc agcaattgtg gacggttggc ttcgctcagc gctagatttc
    26641 ctgaaaaaat aagccctgaa agacaggtaa cagcgatagc catcggtttg ccgacagggt
    26701 tgcttttgat aggtattttc ttgaccttca accaaaccag tagcccaatg aacacagcga
    26761 tatccagcca gtagatgaca tcatggggct tcatcaaaga aaaggagctt cctgacaatc
    26821 cttgcgacac tttcgaaaag cctaatacag atttgactgt aatgaaatct gtgaattccc
    26881 gataaaaaat aatattcaag tacaacaaaa gcgtatttaa aatatcaatg atcaaaagaa
    26941 tagctaacgc tggctttggc tttttaatat acaaagaaag gccaaaaaac aataaggtag
    27001 tcgcaattgg gttgatccac aagataaagt actggatcgt accttccaca cctaatgaaa
    27061 aatctgcata gtaggcaatg atcgtcttca gccagaaaaa gaaaacagct gtcaaaacga
    27121 ttgctaaacg attttggcac aattttttaa gttgtgtcac gtatatcact tctctctatt
    27181 cacatttttt cttattttgc tcctgaaaaa tgaaaatttc ctaaaaaagt tgttatctta
    27241 agattttttt attctttcta aagattattc cgtccacttt tcacagcata aaaccgctgc
    27301 gatgtaagcg attaaactaa tgaagagata aaagtcttgt ggtgaccgct tcactccacg
    27361 gtattcaaaa gccagtttct cggtattcgt acaaaaaaag aggctgtgac aaaattcttg
    27421 tcgcagctcc ttatttcttg atgcaggaca gctttttcac aacttcttcc ttaaacggtg
    27481 ttctcctgcc aactcctcgg ccacaagtca caatatttat aaaacatgag aagctgtttt
    27541 ttgaaattgt tccttgtggc tcagagttaa acggcaggct caagacctct tgatacacgg
    27601 tgttcaatca gctgaccctc ggtattagat tatgtcagga cgctaatttt acaacctctt
    27661 ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaaccaaac gaaaaatata
    27721 aggagctatt ttttgaaatt gctccttgtg gctcagagtt aaacggcagg ctcaagacct
    27781 cttgatacac ggtgttcaat cagctgacct tcggtattag ctcaaaaaac ggcaaaatat
    27841 gaggaactat tttgccgttt gtttctctaa tactcaggtc aaaacgctga tttcacaacc
    27901 tctgtttttc attgtccata ggattgatat tgtccttgtg atgactcatt cgtaataatt
    27961 gaggcttgat tttcttcact tcccgttatc gtttgtccac tgccagaatc tgaaggactt
    28021 gttcctgtcg actcgttttt ttgagaagag acagttgttt tcttgacttg ttcatttgtc
    28081 tttttccatt ccgctaaacg atcggcgacc acttttttgt ctactttttc agaataagcg
    28141 gtgatcactt ttccattttt caaacgggct actttgtcgc catcatgagt caaaagcaaa
    28201 acggtcaaat gcttgtcttt aaattgatat tgtctgagag aaatcgcact aagtacgtca
    28261 tcgatattaa taaattgatt ttcttctttt ccttttggca aataaatgtc caattcgcct
    28321 ttttcgtaat tggcagttat tttatactcc gcccaccaat aagttgagaa ttcttttttg
    28381 aaattatcta ctgattcttt gcttgctacg attttttctg tttctttctg agcaagatct
    28441 gctgtcattt ttcctgaaaa tgctgcggat aaaataagca gtaagaaaaa accgaataac
    28501 caaaagagaa gaaacggtct ttttttcctc atgctttcct ccactcctat ttaattttga
    28561 ctattctatt atatacaaaa aatgaaatag aaagacaaaa aagttgaaaa agatcgtaaa
    28621 taattgaact tctttttcac aaaatggtaa gaatggagaa tttcacgcat ttgctctcaa
    28681 ctattctttc ttaagcctca aatccgtttt tatctgatac gtctttgaac caatgacccg
    28741 attttttgat tgttcttttt ccatcttgtg ctaagtctac tgaaataaag ccatatcgat
    28801 tcttatatgc attggtccac gaccagttat ccatacatgt ccacatatga tagcctaaac
    28861 aattggcacc ttcttgaatg gcttgatgga catatttcag atgagattgg acaaattcga
    28921 tccgataatc atcgtggatc atgccatctt ctttgataaa tcgtgcttcg ttttctacac
    28981 ccataccgtt ttcagaaata aagcagtcga tatttccata atgatctcgt acattgatca
    29041 aaatatcata gattccttgt tcatagatct cccatccgcg ataaggattc attttacgac
    29101 caggcatttc ataattgtcg aaataatcct caggcattgg accgttttta gtatcgattg
    29161 gtgtttcttt tgcttttacc cggcgtggct ggtaataatt gatacctaaa agatctactg
    29221 tattttcttt gataaccgtc aaatcctctg catggatcac aggcatgaga tcaagttctt
    29281 ttactatctc aatcaattca cttggaaact cgccttttac agctggatca aggaatgaac
    29341 gattgaagaa cgcatctgct atttgcgctg ctttaacatc ttcagggttt gattcgtctc
    29401 ttggatagct tggtgtcaga ttcaagatga taccaataac accaccttgc tccatttcat
    29461 ggtaagattt gatggctaac gctgaagcca acgcttcatg atagccaact tggatcgctt
    29521 ttttcatatc tgactcttct ggataatgcc atcgatataa ataacctgct tcgactggaa
    29581 cgattggttc attatgagta aaccattttt ttactttcga accaaacaat tcaaaacaac
    29641 ttttggcata tttttcatag gcttcaaccg tttctcggtt caaccatccg cctttttctt
    29701 gcagatacat cggcatatcg aaatgataca gattcacaaa gggttcgata ccgttttcaa
    29761 tcaactcatc aaaataacga tgatagaaac gaacggcttc ttcgttgatt gttccagatt
    29821 catcaggtat caaacggctc cattggatag atgttcgaaa tgaattatgc ccagtctgtt
    29881 tcatcaactg tacatcctct tgatacttcg tataaacttg cgaggttttt tcaggtccta
    29941 cttgatcaaa gaacttttgc ggttcttttt tgtaccaata atcaaaaatg ttctctcctt
    30001 ttccatcgcc tgaaatcctt ccttctgttt gtgggccact tgctgcagaa ccccaccaaa
    30061 aatttgctgg aaattgatat tttttcatca aagcacctct ttttcttttt tagttcgttt
    30121 tagttgattc catgtatgat attttccatc acaatcactt tttcattttc ttcctcttct
    30181 tttttgattc gttttaacag ctgttctgct gctcgttccc cgattttatc tgttggtggc
    30241 gtgatcgtga ttttcggaag gatactcaga tcgtttttat ctaaataatc atagtgactg
    30301 aacgcaatat ccttcccgat tctgacacct tcttgattga aacattcgat ggcaccaatc
    30361 gacatattgt aattgctgac aaaaatagca gttgcccctt tttctaataa tgcttttgcc
    30421 ccgtaatagc cgctttttct agaatagtct cctgtaaaga cctctaattg gatcgatgga
    30481 tcattttgga ccgcttcttt ggctcctgct aaacgttctc ttgctacgta gtctgtttga
    30541 gtggcagcaa taaatccgat ttttttatgt ccttgttcac catgatgagc gatcactttt
    30601 gctacacttt ctcgatcatt cactaatatt gtatcaatac ctgctggtcc ttctggacaa
    30661 ttcaatgcga taactggaat actcattttt gtgagcattt caaatccaga ccactcttct
    30721 tcggataaga agacaatcag cccatcgatc acatgcttca tcaggtattc aattttttgt
    30781 tcaatttttt ctggctgatc ggcaaacccg cttaataata agctataccc attttcttca
    30841 gctactcttt cgatccctga gacaacttcc gacccaaaac ggctggaaat cccgtttatc
    30901 aaaagtccaa tagagaagct tcgattattc ttcaaccctt tagcaatgat gttttcttta
    30961 tagtccaacg ctgaaatcgc ttctgctatt tttttcatgt tcttttcctt cagttgctga
    31021 ccattcaagt aacgggaaac tgttcccact gaaacaccag ccatcttagc cacatcacgg
    31081 atcgatgcca tacattctca ctcctgccag ttacatttga tttctctttc ttgttgttcg
    31141 attatgatga ccgcattctt tgcatagtgt tgcttgtcat caatatccat ataactaaag
    31201 tcttcagtag agcaacttcc tctcactatt aattatactt aaacaagtat ttgaaccggt
    31261 tcatttttaa tatagcattt ttttaaaaga ttgcaacact tttttaaaac gctttctgaa
    31321 agtggcggat tttttgcctt tttaaaattt tattctaaaa tttactctat ttgtgggtgt
    31381 tttgtgcgtt tttctcttcc atccgtttga agttttacat tccaatggta taatttcttt
    31441 atcctatcaa atgggggaag tattttgaag aaattaatcg tactatttat tttgtctgcg
    31501 ggacttttga cattgactgg ctgtgatttg tttggcggct ctgaccaatc ctcggacgaa
    31561 acaaccgatt cttctgtctt aaaagcggaa aaagcagata ccaccaacag tgtatatacg
    31621 ctaaaagtac gtaaagatat ggatttatcc gcgggacagt tgacatttac agataaaaaa
    31681 atggaatgga cacgcacgta tcagacacaa ggaactgatt cttctgaatc atctgaagaa
    31741 aaaacagtgc tgacagatat caagatcact accaaagatg acgaatatat tatttcagga
    31801 aaagaaaata aaaaagaaac aacagtcata tttacaaaaa tcggtaatta ccgtttgaag
    31861 gatgaggatg gcaacatcta cagtctatag tgccgtctgt tggatcttct tttgtttctc
    31921 cccttctttt ctgctatagt aaagaagcaa gatgattgta aaggagacga aatggatgat
    31981 cgatagtcct gattattgta catgtaataa caacaaaaat atccatacag aatttaatga
    32041 ccaaggaagc ttgagcatat gcgacaactg tcacaaacta gtcaaagatt catttaaaac
    32101 atttgaatct tatcctgttc aaaattatgt aaaaagtaga agaagataaa caaatccaag
    32161 caaaactcac tatggctgat ttttatgaac ttcaaactca gcttcttcct ttgatcaagc
    32221 ttcatcagta aaacaaacag tggatcattt cagtttacaa atcaaagagt aaagatttat
    32281 ttatttcaaa tatttgaata taaaataacc aaacgataga aaggcttatt ctacatcacg
    32341 tagtaatttt tctttcccgt cgtttggttt tttattttta gcctaattct tgaccttttg
    32401 catgctggtt atccgtaaaa tattttccag caggctttcc ttccaattca gctaattttt
    32461 cttggatctg acggatcacc ttttttgcat cttctggatt ttctacaaaa tcgaatttat
    32521 ccccatccac ttgtatttta ggacatacat caaaatcttc ataccattga ttatagcggg
    32581 tgttcaacgt ttcataatag gtatataacg tctcatcgct ttctagctgt tcatagtcac
    32641 gcccacgttt tttgatacgt tctagcatcg tgtccaaaga aactttgata tggaccatca
    32701 aatcaggccg tttttttgga gcaacatcat ctaactcgtt tagcatcgta tctaataagc
    32761 tatcgtactg ctgtacttcg atatccgtta cccgacctaa atcagcgttt agatggaaca
    32821 acaggctatc ttcatagatg gatcggtcta gcacattgtc atcgttcact aaagcatttt
    32881 tcattgctaa aaatcgcttg ttcaaaaaga agatttgcaa taaaaaagcg tactgctcag
    32941 gatttgaata aaagagcggc aatacttcgt tatcttcgac attttcataa aatggtttag
    33001 aatcaatctc ttttgcaagc atatccgtca aactgctctt acctgcacca atcgttcctg
    33061 aaatcaaaat cactgacata caacccttcc tcttctataa gtactcgcct acgtaagttt
    33121 caacaggcaa tgtttcaaaa tcatattcac gtacagcttg gtctgatttt aaatacgtat
    33181 ggagactttg tgtaatcatc aagttcgtag gtaccgtctc ttcttgtaca atgatcatcg
    33241 gttttttcaa catagttgcc acacctaatt cgtaagccgt tcctggatcg attgtttgat
    33301 gctcaaagtc aaggatagca acaacaaatt ctgcatttgt cagttcttcc atatcttttt
    33361 cgtatacttc ctgcgcccat ccagcgctga atagttcata attgctttct tggtgatgac
    33421 gtggagagta gaaacttgtg actgttttgt tttcctccaa cgctttttcg atacgactta
    33481 ctcgatcgat ttgctcctct gaaaaaaatg gtcctgctaa atatatttga ctcattattt
    33541 tgcctccttc aataataact gatttttaat agatactgct cgtaattcat tgaccagttc
    33601 caatggattg ttggaaaaga ttacttgatc caaccatttt ttatccataa aatcctcttt
    33661 tgcaaaagtt tctaataatt gttgcaagcc gctgtaataa ccgccaatat tcaatattcc
    33721 gattggcttg tcgggcgtca gaccgattgc tatccaagaa agaagttgtg agaattcctc
    33781 caatgtacct agacctccag ggaaaacgag gacagcatct cccttttcaa ataataatac
    33841 ctttctttca tcgatcgttt ctgtaggaat aaatgtagta acatcttttc caggcaattc
    33901 agtttcaaaa agcccattcg gatagatacc tgtgactttt ccgttatttt ccagtactgt
    33961 tttactaatc actcccataa tcccggagta tgaaccaccg taaaccagtt cgatttcttc
    34021 ttgtgccatg tattttccta acgtttgcgc aataaatttg taactttctt tattaccaaa
    34081 acgggaaccg caaaaaacag taagtttcat aaaaaaacac tccctaactc aaaagtctat
    34141 cttcattcta cacgtgtttt tttaaaataa aagactagat tttgtagttt tttagtggta
    34201 tataggatag acgccactat atatagtatt tagaaaaaaa tcatttttca gccattttat
    34261 agataaaaca aaaataaata acgtgttttc atcgatatga aatgacttac ttatagaaga
    34321 tactgggatt tcgtcctata tgccaataac gtgtattatt ttaaacaaca aatacttgtt
    34381 tcttttatta acgaaaaaat cattttgttc tcgtgttatg tttcactgat aaatgatgtt
    34441 tctctaaata aaaaagccga cgaactaaac gatcaaaatt tgatcatttt tgttcatcag
    34501 cttctcctaa aaatcttttt gttaacgata aatagctgga tttgtaaaaa tatcacttga
    34561 atcgtcactg ctggaagaaa attcagtgat aaccgcacct gttgcgcctg cctggaacta
    34621 gtgtttgata ttgggctcga acgtgtactg atctctagca tgaagaataa tttctttttt
    34681 tactgtataa taactttctt tcctttttgg aatagtacag gaaagttttt ttgtttcctt
    34741 tccttcgaca taaggtatac cagacccttt cgacaacgaa aggttctcat ttttccttca
    34801 tcgtcatttc aaggagggtg ccgatgctct ggacagtttc gatcgctcct aaaccaaaat
    34861 ctgtatattc gattctttct aactctgatt ctttcatacc attcctgtct caaggtacat
    34921 ctattgtact tttttcttgt aatccattct gcctctccta actaattgtc aaatcatccc
    34981 aagatatgtc ttcttcctct ttttcttctt ggatgatttc tgcattcgga tctactggtt
    35041 ttttcaataa taaaatggtt attcctgtga caaaagaacc agctaacaat gcaccaatga
    35101 atgcccatgg tctggtcatc gtagcgactg caaacattcc cgatgccgga atacggctat
    35161 ctgcgcccca aaacatgctt aatccgccac ctactgtccc accgactccc gttgctatga
    35221 cattgcgtgc caagtcgttc aatacgatag ggaaacatcc ctctgtgatc tgacagatcc
    35281 ccataggaaa agcggtcttc aatgtatcga tttcttgacg attgagtata tttttacgaa
    35341 tgattttccc taagaaataa gcgatcgtca agccgaatgg ggttaccatc gaagctaaaa
    35401 tcaatacagt gattggttca tagattcctt ctgaaaatcg tgccacaccc ccttatccag
    35461 aataaatggt aatccagaaa cagccgtgta tctgcggtaa taagcctaac caaacgaaaa
    35521 atatgagaag ctatttttgt ttggctgttc ttattacttg atgcagggca gctttttcac
    35581 aacctctttt tttatttatg tttcataatc aatatggtaa ggatgcagct ccgtatggta
    35641 gaccgcctcc gaatattcaa tcatttcccc aatttgcgta gacgatttcc gtatccgttt
    35701 gatgccgatc tgtgcgtctg ttttcagaat tccttgttct tcttctgtta aaaatactgc
    35761 cgagaaatca tcagaaaatg agtcaatagt atatcctttt ttcgtcagca aacgatacaa
    35821 agattctttg ccaaattctt ctctgctgaa ttgcatcatt tcttgtggaa gataatgaac
    35881 aaaataaata taaggctgat tgttcaaata atataagcgc ttaaaacaaa cagcttgctc
    35941 accgaaaaag gaaaacaaag gatcttcctt ctttatatgg accaattgca aatccagcgt
    36001 ttctttccgg acttcatatc gggagttctc caagatttgc gtaaagcttg ccgctttgga
    36061 taatcgattg taaggacgat tgcttagaac cgtcgtccca cgaccacttt tctttgcgac
    36121 atattcatct gatgccagca gttcgattgc ctttcggaca gtgattttgc tgacttgaaa
    36181 cagttcttct aattcacttt ctgtcggcaa catcgaaccg actggatatt ttcctgagaa
    36241 aatatcttct ttaattttat ctgcgacttc caagtatagt acacgtgtcc ttttcataac
    36301 gtcacccttt gctttactat tactaaaaga atagcacata aaaaataaaa aaagaacaca
    36361 aatcacatgt gagaagtgtg ttctttttca ttcagctgct ttaattacaa gtttacgcca
    36421 tttgaggcaa ttacttcttt ataccaatca aaagattttt tctttgatcg attcagtgtc
    36481 ccatttcctt catcgtctag atccacatag ataaagccgt aacgtttact catttctcct
    36541 gttgatgcac tgacaagatc gatacatccc caaggcgtat agcccatcaa atctacaccg
    36601 tctgctactg cattagccat cgcttcgata tgacgacgta aataatcgat ccgataatca
    36661 tcaataatcg tgccatcctc ttccatatga tcaatggctc caagaccgtt ttcaacaatg
    36721 aataatggtt tctggtaacg attgtataat tcattcaatg cgattctcag cccttctgga
    36781 tcgatctgcc agccccattc acttgcctca agaaacggat tttttactcc tcccagcaag
    36841 ttcccttctg aagttgctgc ttgtggatca gtctcattga ttgccgtaga catataataa
    36901 ctaaatccga tgtagtccac tggatattct tttagcaact ggaagtcttt ttccgtttgc
    36961 tctaattttt ccggatcgac tccatatttt ttatgggtac gcgctgtgta tgcagggtat
    37021 tctccacgta cttggacatc cgtgcagaaa aaattgaatt cttggttttg gatcattgtt
    37081 gctgcctgat tgactggatt ggcatcgatg ctgtaagttg ttgcatagat aatcatgcag
    37141 ccgatttgga tatccgaacg caattcgtgg ccgattttta cggctttgct acttgcaacg
    37201 aattgattat gccatgcttg gaaaatattt tggtactctc ctgctccatt gctttttact
    37261 aatccttgac ttaaagctgg aaagtgaaag gccgaattga tttcattgaa agtcatccaa
    37321 tatttgactt tactgctgta tctctctaat actgtctgag caaatcgttc atagaagtcg
    37381 atcagttttc gatttttcca gccaccgtat tcttttgcca aatgaagagg catttcgtaa
    37441 tgagaaatcg tgatcacagg ttcaatatca tattttaagc actcatcaat cagctgatca
    37501 taaaattcta atcccgcttc gttaggagta ccttcgtctc catttggaaa aattcgtgtc
    37561 caggcaatcg aaaaacgata acatttgaat cccatttccg caaacaaggc aagatcttct
    37621 ttgaaacgat cgtaatgatc gattcctcga tgattcgggt aaatatattt ttcttgatca
    37681 atcgtccagt caaattcttc actgccgata atcgcaaagc gctgcttacc tccaggcata
    37741 gcatctgcaa cggatagtcc ttttccgtct cgatcataag ccccttctaa ttgatttggc
    37801 agcagttgct ccaccccata aaaatttttc tgaaaaacgt gattgcatcc tgatttcctc
    37861 cttattttac aattgccaga acatcttcgt ttgcaataat ctctttttgg tctaattcaa
    37921 gtatatcggc aaatgcatca gtattcgtaa tgacaactgg agtgattacg ctatagccag
    37981 ctgctctaat tgcttcgatg tcaaaacgaa tcaataaatc tcctgctttg acgagatccc
    38041 cttgtttgac taagatctca aagccattcc catttaattc tactgtatcc atcccgatat
    38101 gaatcaaaat ctccgctccg tcagtcgttg tgatccctac agcatgtcca gttggaaaaa
    38161 tagtcgtgat ctctccgtct gcaggtgcaa ctagttctcc tacttctgga tcaatagcga
    38221 taccttttcc catcacgcca gaagaaaaaa cttggtctgg tacttcactc aatggcagta
    38281 ctttacccgt taaaggactt tttaaggtat gccgtgctga agtaatcggt tctcctgcat
    38341 tcgcatgttt gttttctaat tgattgtctt gtgtttgttc gtcaaatcct aaaatcaatg
    38401 tacctacgaa tgcaagaaca aaagcaattc ctgtagctat tactgctact gtcacatttg
    38461 actctacacc atccaccgta ttaatgaaag tcggaatcgt caataagctg accaaactgc
    38521 tggagaaagc ttttactccg gaaaatccga taatagctcc accaatacct ccagaaatac
    38581 aagcggcaat gaatggtttc ttcaaaggta atgtcacacc atacacagtt ggttcagtga
    38641 ttccaaataa cgaagtcact gtagaagaaa tccctaatgc tcgaagtttc gtatcttttg
    38701 ttcgcaatgc tacagctaaa gcagccccac cttgtgccaa gatggctgga agaagcattg
    38761 gcatcaatac atcaaaccca tattgttcga tattcaaaaa catgatcggt acgaatcccc
    38821 aatgcatgcc gaacatcacg aatacttgcc ataaacttcc catgattaga cctgctacga
    38881 ttggactaaa tccataaatc gagttgaaga gaccgcctaa agcattaccg gcaaccgtcc
    38941 cgattggtcc taaagcaaga aatgttaatg gaaccatcac tactaaaacg acaagcggaa
    39001 ctaaaatcat ttgcagaaat tgcggaatca ctttttttac gaatggctct aatttacttt
    39061 gaacccatac ggctagaatg attggtatga cagaagaagt ataaccagat ggtgaaagaa
    39121 tcactgggat cccaaagaaa ctgatagctt ctcccgcacc agcaactgct gtgatagctg
    39181 gatacacaag tgccatagca attgctactg ctaaaaactg gttggctttg aactttttag
    39241 ctgctgtaaa agcaagcatg acaggtaaga aggtaaagac accatctgct gcagcaaaca
    39301 atatttgata agtaccgcta tcagctgtca gccagcccat caccgttgcc aatgataaga
    39361 atcctttcaa taccccggcg gctgccatcg cccctagaaa aggtgtaaat atactagaga
    39421 taatatcgat cagacgattc aaaatatttt tggactcttc ttttccatcc tccgaaactg
    39481 caagaccttc ctcatccacg atcgattgat aaacatctgc tacattactg ccaatcacta
    39541 cttgatactg accgccactt tgtacgactt ggatcacgtc cgggtcttct tttaactttt
    39601 gcgtatctgc taaagattca tcttttaatc tgaatcgcaa ccgcgtagca caatgtacta
    39661 aacttttgac attcttttgt cctcctaccg cttcccatac acgacgacca actgcttgat
    39721 tatccattgt tttttccttc tttctttttt atttaaaaaa taaaaaccca gattgaaaaa
    39781 aacagtcaca aacattcgtg aacctatttt ttcaatccag gttttgcctg ccatacagta
    39841 acaatcctta tgattggatg ctttttttct cacttactac tctttgaata tgaatcgtta
    39901 aataaatctg ctctgcgatc cccatttcct gtttttgcgt ttgtcgaaga tactcattga
    39961 tccgttcgac acaatgaaac gcttttggat actttccttg aactaatgcg tagagaaatt
    40021 catcttgctc gtcagatcct tcccctttga gataacgctg ggcaaaaaac tgcagatgag
    40081 taatgatacg ctgataattc aaagattctt catcgaacac gatgccgaag aatttactga
    40141 tgatactcag tatatctctt accattgtgg tagcggtcat cgtcacttgc atgtcttcgt
    40201 ttccttgttg gctattcaca aaatgaaagg caataaaacc ggcttcatcc tcagaaagtt
    40261 ccacacccgt cttttgtttc acgaacaaaa gagcctcttt cgctgcttga tattctttcg
    40321 gataaaattt ttttgtttca aataataatg gattaggcag ttggatattt tcttttgctc
    40381 gtgtgatcgc ataatgaaga tgatcggtca atgtcagata aatattaggc gaaaattcaa
    40441 cttttagttt atcttcagcc agttcgacta cttgtttgac ccattcgatc tcctctgccg
    40501 gaatatcagc catcagctgc tggaattggt ttttagcaaa agaatctctt aaacaaattc
    40561 tttttggacc gcttctggcg aaatctcgtc cccaatcttt ttttgaaaag ctagtccttt
    40621 tcccatacaa acgacttctt ccccgaatcc atttttcgaa atgacgacat tgttattcaa
    40681 aattttatca attttcattt tgcttcgccc tctcaagaaa aataaaaaaa cctagaattc
    40741 gcctactgtt tcttagctga attctggttt tgcctgccgt acagtaacaa tccatttctt
    40801 attcaaaatc atcataccat cgaattttta tttatgcaag ccttttcata taaaaaataa
    40861 aaaaactgga acacaagtct atctagaaaa caaccaaatc agagatgcac ctctttgcgt
    40921 ttctcttttt ttcagcaaat tggtttttcc ttatcgattc atatcctttg gctgttttta
    40981 atctgtaacg acttatgtcc cagtatccta aaatgttttt gtctctagat tgaaagcaga
    41041 tctcatatca tctgtcgcat cttttatcga tgtaacactt tccagttctc cttgtacaac
    41101 tagtcgcgtg acggcgtcca tgtcgccaca agtaattagt gtgacaattt cttttccctc
    41161 gacttcattc agtaattggg tatctgtagg agcaactttt tctttaaaaa atgttttgta
    41221 tgtatacaca ttttctaaat ctgtcaaata gatcttggca cccatctcta aattttctag
    41281 cggtgtaaac agcaattcag ggttcgttgc acgatggctt gctaaggcat aatttccttt
    41341 tcccatttct tgatctggag ataatgttcc tgctccgtaa agtagtgacg tattatccaa
    41401 gcctttgaaa accggtaaat tgattgaaac tgaagggact gcgatactag caatcactgg
    41461 tagatctgtt cctctcattt gcgacctcat gaccccttct gtcgtcattg gttctaccgc
    41521 atcaaagtca aaactgacat ctttatcgtt attctttttc agatcttctt ttgttacatt
    41581 cgctatagca tatttatctc ctgtctctct gacaaaataa tccttgatct gttcattgaa
    41641 aatcaaagct agtcctacta ataacaagag gaacaagagg atattgacta gccatctttt
    41701 tgcttttcct ttttttttca ttttctctcc ctccagactc attttactgt ttttcttttg
    41761 ctctttccaa cagccgaatg acacatttct acaactctgt gtattttttt cgtacacctg
    41821 cgcttttttc tacaggatat ttcaaattat ttttgatcaa tttttcttca atgatctcat
    41881 ctagatccag gtgaaggttg tctgccatca tgtatgaata gatcaataca tctgccagtt
    41941 cttctttgat tcgttctaat tgtgtttctt ttacttcttc cggcgttttc cattgaaaaa
    42001 gttctaacaa ttcagatgct tccaaagaaa tagagatagc caaatctttt tcgttatgaa
    42061 attgtcgcca attacgttca tctctaaact ggttgacttt ttccatacta ttcatttgct
    42121 tgccttcttc caatcacttc ttacttgatt aatcatacca tttttttaca taaaaaatag
    42181 atgatgcttt taaaaaaact cctgttttct tttatttaaa ataaatgtca tagaaaatca
    42241 ataatttagg tagccaccat gtgtatgcag tagcgtgtct tcttcccttg aggctttttg
    42301 tttttcgata cgttcttctt cccaaacaga caacgctact agaatctctc ttaatgattt
    42361 tccttcttcg gtgagcgagt actctacttt aggcgggact tggttatagg cttttcgatg
    42421 gatactttca tcttgttcta actctcttaa ctgctgggtc agcatttttt ttgtgatcgt
    42481 agggatttgc ttgcttctcc acgcctaaaa taagcgatat tgctcttccc tttatctggt
    42541 gtaagcaaag cagttagcat tttgacgatc gtgggttccg cttttcactt cttatctact
    42601 tagtatcatg aagtatactt tcgtactaat actaagtagc ggaagtgtca actactttgt
    42661 ttaactacat agcaaaagaa acagaagggt gacaacggtc atcacgcttc tgtttctttt
    42721 gaatatgctg tgttgttgtc tctatctccg tgtagacttt cctcaaactt atcctttgac
    42781 agtaagtcag taggctgatt tgctctttct agatgccaga tacatttcta caaaaaaata
    42841 gtgattctgt tttgaaaatt cgatttttag ttctgcattt tcgtaaacat ccaacatttt
    42901 tttcgcactg tatagaccta acccgccatt ttttttagag gtttttcctc ttttgaccat
    42961 ctccgatagc gtcaaattcg ttggatttgc cttgttcgct atactaaaac gtgatccctc
    43021 tttttcttcc agcagattga tcgtaatcgg actctgatcg gctacacgat gatcgaatgc
    43081 gttagaaata aaaatagata aacagcgaat aaaaacaacc agatcaatcg ggattgtttt
    43141 cacttcgaca ggaataacta cttggatcgg gattttctcc gattctgctt tttctagaaa
    43201 ttttaaaaag acacccctga cagctgggga tacaatattt gttagctgtt gtatttgcgg
    43261 tttttcaaaa atagctgcag aatgatcttc taatatttct aaaaattcca acgtttcttt
    43321 tttattttcc tgaagagtaa tttttaatga aaacagtaga cttttataat catgtttgaa
    43381 ttcctctaga tgatggtaat attcttcatt tatttgatac agttgaagcg attcctgatg
    43441 ttgtctttct tttacttgga aactgatcaa cataatggaa aaaaacaaag aaaaaaaact
    43501 gatgactatc gacaaaagag atagaagcag ataattagat gagcggaagt gaacacttcc
    43561 caataccaag taaatcacgc cccagcttaa taaaatacaa atacctccaa aatagtgttc
    43621 agttggataa gtaaaaagca agttgagaaa tctcgtctgt ttgtctatac tttttactaa
    43681 tagatagaaa ataaatgcga taagtagtgc aaaaaaactt gctgttccaa gatgtctctt
    43741 tcctaaataa tccatcagat caaaaaccag aaaaaagctg agtccagtga tagcaaaaaa
    43801 aagattactg atcaaacagc tgataaacag gtttttcgta gtaaaataaa aaagaaacat
    43861 caataataag ccaactaggt aaggaaagct cactttaggg ataggaagta aaagtgtcac
    43921 caccatcaaa gctatacaac caaagctgtt ttttactagc agttgtttcg aaaagaataa
    43981 atagatacct aaatctaaaa tcaaagtaaa ggtcaaataa agtgtcactg ttgcgaccat
    44041 cttattcctc cattgttttg tttcaaaatg tacacatatt tttttaatag gagcctgcat
    44101 atgaaaaagt attttatcat agaagataac cagtttcaca gcgaatggat catcagagaa
    44161 ttggtcaatc atcatatcga gaatatcgtg cagctcactt ctatctctca aatcaatcag
    44221 ttgatccagt tgggcactat tgataatcac gatgttttct ttatcgatat cgacttgaag
    44281 caatcactca caggtattga tgtcgccgaa caaatacgta aaaaaaatgc acatagtaat
    44341 attgtttttt tcaccgctta tacgaatttt gcagtagaga tcatcaatcg gaaaatcctt
    44401 ccgattggat atatcagaaa agacaggaat ttatctgaac aaattcgtca agtgctagaa
    44461 cagatcaatt ggaaagaaca gcagatcgta aatgtcgata aaatctttat tgatcaaaaa
    44521 ggtggaagtc tcgtcctgta tcctcgggaa attttatata ttgaaagtat caaaggtttg
    44581 aaaaaccaag ttatggtcaa aacaactaca gaagataaat tagtgaatac ttctttaaaa
    44641 caattgaaga cagagttaga aaagtatgct tactttttac ttttaaaatc ttactgtatc
    44701 aacttatcgc aattgcagaa aattgattct gctcattatg tgttagaatt tttcaacggt
    44761 gactccttac tcgttggtcg aaagatcttt gaaaagacaa aggaacgttt ccatgacttc
    44821 caaaaaactg cttcgagttg atgaataaga gctgagacgt aagcatagca gatttcaggg
    44881 agtgggcctt gaatcggctc cactccctga aatcgttgat atgattttac gcttcctctt
    44941 ctgtaggagc atctgttaac gaccatgtga atgttgaaac gtagttgtct acagcataat
    45001 tggatacagg aacaaataat tccactgatt gtctcccttg ttccacattc tctcctaatg
    45061 aataaatcca tgtgccaatc ccttcatttg ctcctgcagt tactagatct actgcctgat
    45121 ctgctgggat ttcgattcct tcagcgccag ttccagaaac ggtagatggc attccactgc
    45181 tattagttgc gctatttcga atcacggcgt ttttgaattt cacctttgaa ccagttagtt
    45241 tattcgtatc agatgttgta ccaataaatt ctgttcgttt gactgataat ttccaaccgg
    45301 cataatttcc gcttttatcc gttacttgta cgaaattctg tacttgtgtt tgtgctccag
    45361 tactgtccgt tccgctttgt aatgcagcat atactgtttt tgctgtacct aacttgattg
    45421 ttccaaaatc aagatctgga gcttggtcaa tcgtcagtaa tccggatgtt ccctgatttg
    45481 gttcgtcagg tgtaaaagga gtatcaggat catttggatc aacaggagga actggaacaa
    45541 cgtcttcagg atttgcagca tctggtttgg tcacagaaat cgttccttct gtagtggctg
    45601 agctcttatt ctcagcaaaa acactttgtg tacataataa tagacttcca gctagaacgg
    45661 tacttaatgc aattgttttt ttcatttctt tttccttctt tcttttaaaa taaataatcc
    45721 agcaaataag ccaataagaa tgactccaac aagtatcagc catcgccagt caaatgtttc
    45781 tggctgattt tttaaatcaa tatccttttc attcaattgg tccgctgcat catcagttat
    45841 ttcaaaatcg gtatggagat tccagcgttt ttctttccaa gcacccttca caaccgcttg
    45901 ataatgtccc ggagctaatt tttttccttc taatgggaca agtaaatcaa aatttgtatt
    45961 gggtgctaca tctatttttt ctttttttat tgaaaggtaa ggctcttttg tttctttttt
    46021 gtagatttca accgatgtag tcaagccgaa catataagaa gcatttggat tttgaagatt
    46081 cgccttaatc acatttcttc catcgctttg atccgcataa gcttttttta atttcaaatc
    46141 tggggaagac tctttttcgc tatttcaaat caagattgct ttcacttgag aaaactgatt
    46201 gacgacacca actgtacttt tgttagaaac gttctctttt ttttcttgaa acgtgatccc
    46261 tcctgcgacc acaccagcca gctctttttc tggcgctttc agtgaaagtg tctcagtgac
    46321 cgcactttga ggaggaacag ttatttctcc cactgttttt gctaagtttt tcatatcata
    46381 acttaatgtt tcatcaaatt tactttttga ttctccatat tcgatcacac cgtttgaatt
    46441 cgtcgtagcg gagtggatca atgtctcaac tgttactgat ttatctgttc ggttaaagac
    46501 cgtgtagggg atttcctgtg ttttcccagg ctccatcctt acatttatat atgttttgtt
    46561 cttatcaatc tgattagccg gtaattcaaa atctaccgca aaattgaatt cggaggcatg
    46621 gacaggtgtc tgccaaagga tgaatccaag gagaaggatc ataagtaaag agcagttgtt
    46681 tcgatgactt ttattccata ttcgattcat caatcatcct ccttgatctt tattctgtcg
    46741 gcgcatctgc taatgtccat gttatctctg ctgagtaatt accagctatc tgattttctt
    46801 tggttaagac aagtttaaac gcccgaccgt tatttaaata ttctgaataa tctgcttgca
    46861 cattcgttga attttgtcct ctgacaattt ccagttcttc ttcattcact aattgatact
    46921 cctctccttt cttgaaatat aggacatcct gcatagaaaa tcctgcatca tttgtgaaag
    46981 gctgagataa ttttatcgtt aagatccacg gatttttcgt ttctcgctga tcataaatcc
    47041 ataatttttc ctgtatatcc atttggccaa atgcttcttg tgtcaaacca gttgctttga
    47101 tctgaccaaa atcgagtttg gaaggggcaa gaaatgagag accaccttct actacgatct
    47161 tcgtagcagg tgcaaaaggc ggggcatcat gataaacaac tggcgtatca cttggattgt
    47221 gattcccaat ttcatcgttg gtacttggct tgttctgcac acccttagca gtagcttctc
    47281 ctgcttggtc gtttaataca atttctaata catctcctgc aaaaagttga ttttctggga
    47341 tagaaagaac aaattcccct gtatcatcaa tagttgtttc tgacaaaaca gcttgatttt
    47401 ttttcaatga aaccaaggct cctgcttcac ctgtacctgt gatcgtgcga gaagctgcat
    47461 tgatcaatgt ggtacttaaa ttagcaggtt caggaggaat cacatcgact gtcgttttgg
    47521 cagagtccag ttctgtcact tcttggccac ctcttacttt tttagcagca acaacttgca
    47581 cttgatcctc cgtttttaat ctctcatcat tgggtacttg aacttcgaac aagccgcctt
    47641 gttcgccttc tccccatggg ttatatcctg ccccttcatt ttcatctgtt tgtccttgcg
    47701 tgagagcagt aatttctaca gatgatttcg tcgtatctgc gtaagtaata cgtaaagtca
    47761 cttctacttc gcctgtccaa gcatctcttg gctttgcctc gacaccttcg ggaacaacga
    47821 catgcccata gattttttta tctgcattcg ttggcactct taaggattca atcatcggga
    47881 gctgattatt ggcactgatt cgattataat ttttcattcc ggcacctgtc tgagcaaatt
    47941 catttgttaa acctgcacta gggcttgata cgaaattgcc taagtcggtc ccattgatcg
    48001 ataagtctgt catatcagaa aaataatccg ggtctttttc aaagttatcg aatgttgcgc
    48061 ctgtttttcg ccataaagcg atttctgaat gggcgatctt taaagaagaa tctccatttc
    48121 cttggaatac ccagccgcct atgccattgg tctcgcttcc aatccgtttg ttttggaaat
    48181 caaaataggt aggagattct aagtccacat tcaacacatc cccactgatg atgccggtct
    48241 tatcttcccc tgttttcccg accatgacta aataagtttt ttcacctgcc gtaaaattta
    48301 aatcaatatc tccagttgta tctacagttg ctccggaatc tgcgcgtata tcgatatttg
    48361 atttttctcc agtgactgta aaatcagctg aacctggact ttctagaaaa ccagaagcgt
    48421 cataaaattg gatcgcttga tttccttgat tgagtctgtt cacgtaagat ttgccatcac
    48481 ctgtatttct taccctcaat gagcctccat tggttactgt cagcttttgc gtaccattct
    48541 caaaacgaac tgcgctggaa cgcccactct gcttatcgat gtcgataact gatccggctc
    48601 cttctacgat caaagaaagg ccttttccta caaaacgcat cccattgttc ccgggttgat
    48661 cgtcatctcc agccattttg accgagattt gtccaccact tgatacatgg atttgactag
    48721 aatctccttg caagcgaaat ccgcccgtag ttgctgtatt gatggtgatt ttcccaccat
    48781 tcgatacagt gactttcgat cctgaacctc tcatttggat gattccgcga ttgttgtcat
    48841 tggtaatatt gatctttgta tttaccgaaa attcgcttcc cgaaccactg acttcaaaca
    48901 tgacattacc aggtagcgta tcgactgcct ctccttcatc gctaacgcca tctgcccgat
    48961 cataatcaag agagaccttt gaaccgctta agatatggat tcctggcgct gtttttccac
    49021 taccagtctc atctcctgaa tctagaaaac cgtagaattt ccctagatac ggtccataaa
    49081 gttgactacc attgatcaca ttcaattgat agcttctaca aaaataggct ttacccggag
    49141 cggcattcag tccttccttc actggttgga catccaacac accggaaaca ccatctaatg
    49201 tgacagttgc acgaggagcg taaatcagtc ctaatttgga cccatcatac aaatcaagtg
    49261 tcccggtaaa ttttatttct cccgtaaaat gatatctgct tccccaaaaa tcggtcatat
    49321 aagcaatgat aatggcattt ccatcgatat ttggtgtcaa tccacctgga tgtgcagctg
    49381 ttcctttaaa attctggaac tcgatcgact gaggtatctc attgatataa atatggtgtg
    49441 tgccgaaatt gtgagcgaat ccaccaaaat caataacgat atctcgtttg atctctaatt
    49501 ccctgtctaa agtcaaaggc tgaatcagtc ggatcttagt tactgattcg tctgcgagaa
    49561 gctgcgccag ttcttctgtt gtggcagctt cgctgtaccc atccgctgaa acagctcttg
    49621 ccagtgccgc ttcactgcta tcagacgttt gttcagctac ttctgaggtc tcacttgtct
    49681 tattttcttc ttgcctttta ttagtagaag cctcactttg agattctgta gtttcagaag
    49741 taatcgattc ttctgaaaaa gtagtcttgt caaaactttg attctctgat tgcgttgatt
    49801 cgtttgtttt ctctacatcg gcaaaaacgg atgtcaccat caagagaggg aagacaaaca
    49861 aaatcgacgc aatcagcttt tttacacatt gattcattct ccaccatcct ctctgacgtt
    49921 ttgacaattt tattttatcg ttttaatcta tttccaattg tactttcata tttaaaatat
    49981 gtatttttat ggaaaaattt ttctattagg tgtaaatttc ttttttgtac aaaaaaaagg
    50041 ctgtgacaaa agtcttgtca cagtccctta tcccgaacaa gcggtgttct cccgccaact
    50101 cctcggtcac aacctccatt ttaacaataa acctgtaaat gtgagggcaa tactttgatt
    50161 gtcacaggta atttatcccc ttcatctcca tcaacattcg tcgtcaattc ttcctcatca
    50221 gctaatgaca caactaattg ctcacaagta aggtaggcta gatttttcgt agattcatct
    50281 actcctttta ataggtctgg caatgtttta agcgtatcga tcaaagaaga atcttttaaa
    50341 taaaccaaat ggagcttacc atcatccact tttgcatttg gaagtaatgt ttcaaaaccg
    50401 ccgactgagt tagttagtcc tatcagcaag gtactgctct caatttcttc cttctttcca
    50461 tcaacttcta cgtgaaacga gtaggattgt gtggaagcta actgtttgat tcctgacatg
    50521 aaataagcta gttttccaaa tttcgtcttc ttctccgcat ctacatcatt gattgattca
    50581 gggatagagc caattgcgac gacattcata aaatagtcgt catttatttt tccaatatcc
    50641 aatggcttga ctgattcaat cgaaaaatga ttgatcgctt cctcaggttc tagcggtatc
    50701 cctaatgctc gagccaagtc attcaccgta cccaatggga aaaatccgaa atttggccga
    50761 tgttcttgtt cagcgattcc actgattcct tcgttgactg ttccatctcc gcccatgaca
    50821 aacacactat gatacccttc cgtagccgct tcgcgagtaa agttttttgc atcccctgct
    50881 ttttttgtat gcaagacgac cacttcatca aacaccgatt ccaattttgc tatcgctaat
    50941 tgctcaaatt cctttgcttg ttcaccgcct gaactggggt ttaccactaa taaaactttt
    51001 ttcatcactt tctttccctt ctgattgatt tctttaatat aacgatagca taaagaaaaa
    51061 aattaggctg tgatgaagta ctgttcatga aacattcatt tattttttga caagataaaa
    51121 catccatact atgaaactga ggaaaattaa aacaatgata taggaaataa aagaaaatga
    51181 taggcattgt atacctaata aagtaaaaat cttaggtggc agataaaaac acaaaaatag
    51241 caagagaatg taatagcgtt tcttccactt tttgttatca gaccttatag cgatcagagg
    51301 aataccaaat gcataactga aaaactcttg atgtactgtt aatatgtcac ggccttgaat
    51361 aataaatgcg acaatccccg ctataattag tgtgcctaac accaaaacgg aaaattgatg
    51421 aatatatttt atatacaaat gatccattgt acttcctgcc tttttatttg tgagaaaaac
    51481 aaatccgtat acgtgaataa cttctagaaa taaaagaaat ttgcaaaacc actccattta
    51541 tctacttcta cgtgcatttt ttgatattgt tttctttatt taagattata cagaaaaata
    51601 tctagggtcg ctggattcta agtaaaaaaa atttttaaaa taaaaagaac taaatcaagt
    51661 agtaattaaa ctactgttga tttagttcta ataaaaagat ttagatgaac agttttattg
    51721 atttaaatag ttcatatttt ttatttatac cgcttattta ctacttgctg tactagaatc
    51781 tgaagtgaat gtactcaaaa cattttcaaa gtctttgtct ttgattttta cattagcgtc
    51841 tttcaatact tcgccaatta ctttattctg gaaggtactg tcacttaatt ttgtgtctgt
    51901 tgcaatttct ttcaattctt ttttgtattt atccatgtcg ttgcctttgt tttggttttt
    51961 caccatttta accacataat attccgtagt ataagttgaa gcattggtag aagtgatcac
    52021 atcagaaact tgaccatctt tcaacttaaa ggctgcttct ttgacttctg ccggaaccgt
    52081 tgtagtagtg gaatcaaatt tcacttttcc gccatcttct tttgtcgtag tatcagttga
    52141 cttgtctttg gctaatttag agaaatcgtc tcccttatca gcagctttct tcacatcttt
    52201 tgcatcatct tcacttgcta cttggataat ttgagcttcc acttctggat ggaaagattc
    52261 ccaagccgtt tttaaatctt catcggtcaa tttgatatgt ttctttagac cttcttgaaa
    52321 ggctagactt tgtttgatac tgtctttgaa tgtttgttct gtgtaacctg cagatttcaa
    52381 ttgtgaatca aatgaatcac ccaattgttt ctttgtttga tcatattgag aatcgatttc
    52441 tttggttgat acgtcatctc catatttatc ttcaaatact tgataaacga tcaactgttg
    52501 cacagcggaa tctgctcctt gcattttttt caagctatcc attacttctt gttcgctgat
    52561 tgtaccgcct ttataactaa cggctgtttt ttcaccggca ttcgtacagc ctgacaataa
    52621 gccagtgcct agtacgatgg caagtgctaa aaatcttttt ttcatttatc aattctccta
    52681 cttttcgttt tttgcgaatt gctaaaaaca aaggtgttca agatgaaaat catcaagaac
    52741 acctttacta atctgctcga tacgtcctga atagatataa ggctacacaa tccagaaagt
    52801 tcgctcagca gaataaatgt caagtagcac atcacacctt aatatactac ttcttttgtt
    52861 accgtttttc aagtctatct tgcgttccgt atttaacgta actaattctt agaacaaatg
    52921 ttatcgattt tgtttttctt cttccgctgc tgggatattc aaatcagaca cttttatgtt
    52981 cgattttcca gacatttttt tgttgttttt attatgatat atttttatac aactaaaaat
    53041 gaatatatta aaactaccaa aaataacttt taacgctgtt tcattatatg ttttttctat
    53101 tttatataaa cttttggata cttaatacca accggttgga ttgtaccaac atgagaatgt
    53161 gtcagttcta tttcctgtcg cacttgcata ggcccatact ccttttgttg catttgctga
    53221 tcgaatagag tttccaactt tcgcttttgc accgtgacga ttatagacac gactatagtg
    53281 gttagaatga cctgtttttc tcgtacaagt tgtatcacta taataaaccc atcttccaga
    53341 gctatgatta attgctgttc tagaaatttc ccggtctact ggtaaatttg acttaataat
    53401 attaccttta gcatcaaaat gaatctctcc tggctttaaa gatttgtaat agatttcacc
    53461 gtgaactgaa tttgcttcca cttcagatga catagcgttt cctccaacaa aacctatccc
    53521 tagtgcacaa ataataactg cttttttcat aaaacgacat cctttccttt ttgattttta
    53581 aagcatgatc ttcttgtttc ctattttggt cagatatgga tcatgcgtga ctacgataac
    53641 taacgtacct tgggaagctt gttgcgataa gatttcaact actttatcct tatttctttt
    53701 atccaatgac ccagtcggtt catcggctaa aatgattttg ctattcttca ataagactct
    53761 cgcacaggcg attctttgct tctcaccacc agataatgtg tagataggcg tacttagctt
    53821 cttgtttata tttacttttt tcaatgcctc atactttgct gttcgcttat ctgaaacatt
    53881 taaattcgaa aacttcaatc caatatctaa attaaaatcg attgtttcat attcaatcaa
    53941 cccaaaatct tgaaaaagaa aagatatggt atttcttttt atcaagcgct tctctctccc
    54001 agttaattgt ttcgtctctt gatcgaacaa atagaattgt cctgaactga actgatcatt
    54061 caatcctaat atattcaata acgtactttt acctgctcca ctttctccaa caacagtcac
    54121 aagatcccca gcatccgcag tgaaacgatc caaatgaaga atcgttttgt ttcgaatgat
    54181 ttttttcgca ccttttatca ctatcaacgg ctccaattgt taacctccat tcatcagcaa
    54241 taactgactt ttcttttctg tagacaaaat aaaaacaact gaaagaataa aatcaatgaa
    54301 gctaaagaaa aaaatattaa aaagatattt aggctctctt tctataagaa aaaggaagat
    54361 tacacttgtc gttccccatt tgatgaggtt catcatgaag aaatctttat acgtttcaaa
    54421 gagcgaataa ccacttgaac gaaagatagc gattttccga caattgtaga caaaatatat
    54481 gcataacatg acatagctca acaagaagaa agaaaagaaa gtgagtaaaa atatctgtat
    54541 caagtaatga aatgatctgc ttaacacttt gacagctatc tcttcagtct gagtatacgg
    54601 aaatgataac tgcaagttat cacgtaactg actttcttca atcagctcaa ataacctttc
    54661 tttcgacaaa gagcctatct tgatctttaa cggtggatag atttctccgc tgagaatatt
    54721 tctttccaaa tacatactgt ttttcaatgt cagtatttca acaaccggat aatgttcgat
    54781 ccaggggata gaaggaataa atgtataaat gggttgattg ttttttataa atagaaattt
    54841 cggttctttt tcactgattt cttgataata ttctgttatt tgcgccaggt cattttttaa
    54901 atctgtatgt ctatatcgtt cagggatcaa tataacaggg tgcgtttctt tttcggttat
    54961 ttggatcaac cttccagaga catcaatcaa aggaaatttc ttaagataat tcagatttat
    55021 ttttatccct ctgccaaact gttcattttc ttcattcaag taatctgata cattgactaa
    55081 tatacttccg tttttattca aagtttcata taattcctga tctgctgctg atgcaaaacg
    55141 attggaataa ataaaatcca cgtggttttt cccgatggtt actggataaa atacatgata
    55201 gtcctcacca gcgatttctt ttggtggttc atgggaaaca gctaactgtg aagaagaaaa
    55261 aacgatagtt gctaattcga cattggcaac ggtcaaaaga agagaaaaca acaatccaaa
    55321 tgtaaaaaga atgtattgga tatagttgaa cgcttctctt aacgaagcga ttttttcaac
    55381 taataaaaga gaaatcaaag agataccata tattccgctg aacaagaaaa cgtacatctg
    55441 gaacaaataa agaaattgct ccttataata cataaaaata aaaccaccaa tgattcctgc
    55501 aattatgaat gtaataatga ttttcttttt ccccaataaa caccacaata cttgatgtaa
    55561 tgaaaagtca ttattcaaca ataaactgat ttctttattc atcaataaga aaaaggatga
    55621 taacaaaagg aataaaagaa tcaatccaat agttaaatac actcgataat tatatacgtc
    55681 tgtatttgaa ataaccgaat acgaatagct attttcaaag tcttcaaaaa tattagtggc
    55741 ataatcagtg tgaaatgctt gattgaatcg atcagtgaat agtgaaatga accgttcata
    55801 ggcttctttt tctctcgtca tcaagaaaaa ttcaccttca aaatctttta ttttgatcga
    55861 ttcttcaatg gacgttacgc gaaaggaggt attagaatag ggtaccttct cgtgagaaga
    55921 tttgacatcc ccttgataat acaatgtcat ttcaagcttt ggctgtatct tgaagttcca
    55981 agatgtatct acttcattgg attcatttac tgtttttttc atataagaaa gctcagtttc
    56041 tgatgagaca ttttcaaata ccatgattag ctgctcatat tcttcttgcc caataaaatg
    56101 ttgtggcaat cgaatcggag tataagtatc tgtttgatgg aaaaattcat ttgtccgata
    56161 gagtacctgt ttatttatac taaccaaggc aaaaccactc atgataccgg tcactatgat
    56221 tgtgagtaaa actgtgaact tttttttgat tatcaccact ctcccttact taaatgattc
    56281 tttgataaaa tcataccgtt tttcatcaaa aaaacgtatc tatcaagtgt ataggtcatg
    56341 tagaataaat ctatatactg tagaatcgaa aggagaagaa gatgaacacc ggagaaaaaa
    56401 tacgaactta ccgcagaaat tgtaagctta cgcaaaaaga attaggcgaa aaaattggtg
    56461 tttccgataa aacgatttct agttgggaaa attctcgtac aatgccagac cttgaaatgc
    56521 tgtcactttt acatcatact ttaggtctgc cgattgattt tccttctatt gaatcagcta
    56581 ccgaatcaat agaaccaaca ttgccagatg aacctcttgt taacaagaaa tcttattcat
    56641 caaaacaacc cttctcactc aaaaagacac tatctttctt tctgctcgtc ttttgtttgg
    56701 tctatctttt tcttatcaat tatcaataca aaaatgaaac gatcgatcga ttcaatcctt
    56761 ttttgaaacc acaaatcggc tataccacac tccctgtagg atacgtggac aattccatcg
    56821 aacgttgggt atgtaatgat atctttggcc atggtacatg gctgtctttt cacgctggag
    56881 aggtagaaaa agagaaaaac catgctgtaa tcaaacacaa aggaaaacac gtcatgaacg
    56941 ttcgcctgct cacttgggaa gccattccct cagattacca agaaatcctc agccaagact
    57001 atgacgacgc cttaaaaaga agccctcagg agccagatag gcattaagta ggagatgaac
    57061 gtagagatag aaaaaatcaa tgcacatgat cttatccaaa aaacaggggt acaaattaat
    57121 tgaaaaagcc ttgagatgaa agttcctaaa taaaaaagtt cagaatgata ggtatttctg
    57181 gcttatagta taatatttac gccacagcct cccaatgatt ctttttggaa tcataatcaa
    57241 ggatggacat tcacaaaaat attttgctca ttcgatccga atagattaac gaccaatcta
    57301 gtaagtaata tagattggta cgtagtcaag ctgcacataa tttagatcaa ttttagttct
    57361 tttttatatt tctatgatct gctcatttac ttttatatga tggcttacag taacataaat
    57421 aaatatgata atcgctggta gtttatccaa gagtactcaa taaaatacaa aatggattgt
    57481 tcaagcgcct atcataatga taaaatgaaa caaataaacg atacaaggag gaagagactt
    57541 tgagtaatgg cagaacaatt ttttcattgt aaaaattgtt cagttgttaa tctatgggat
    57601 ttcggtagca tgggcatttt tttatagagg aatcacacca acctggccgt tttatctctt
    57661 gttagtggga atattagtag gaaattttgc ccctcttcac tatcgtacga acttttttcc
    57721 aactgattct ggcctttttc ttaaaacaaa gacgcgaaaa ttagaaaaca ttattgaaat
    57781 gacaattacc tgtataatca ttttcttagt tatattccta aacgtcttct aataagaatc
    57841 tcttaaaatt agcttgttac agttagttac ctagtttata taacacagtt atcttatctt
    57901 ccttatcaac atcttttgcc aactctttgt cttaaattcg aaacatataa gcagagctac
    57961 gaacaccaag actgtttcgg ctctgttttt tgcatcaaat tcactcttat atagctgata
    58021 atctcggctc tttaaaagaa attattttcg cttcatttta ttataaaaag gctaggaacc
    58081 ttgatgttat caggttccta gcctttattt aagtattacc ccacagaccc ttccatatag
    58141 atttttaaat tacacactag tttaaactaa aacaaacaag gaaacactta ccatttaatc
    58201 ataaaattag ctcatatgaa tatattagaa tgtaaaatag actggtcaaa atctggtcaa
    58261 aaaagaggac taatacaggt agtcctcaga ctgtagacaa agtccatttt atggatgat
//
LOCUS       DANJMI010000015        57698 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000015 DANJMI010000000
VERSION     DANJMI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.015"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(93..1136)
                     /locus_tag="PWU81_001077"
     CDS             complement(93..1136)
                     /locus_tag="PWU81_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDL1109799.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1513..2478)
                     /locus_tag="PWU81_001078"
     CDS             complement(1513..2478)
                     /locus_tag="PWU81_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL1109800.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2712..3557
                     /locus_tag="PWU81_001079"
     CDS             2712..3557
                     /locus_tag="PWU81_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL1109801.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3748..4935
                     /locus_tag="PWU81_001080"
     CDS             3748..4935
                     /locus_tag="PWU81_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL1109802.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4941..5297
                     /locus_tag="PWU81_001081"
     CDS             4941..5297
                     /locus_tag="PWU81_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDL1109803.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5299..5637
                     /locus_tag="PWU81_001082"
     CDS             5299..5637
                     /locus_tag="PWU81_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDL1109804.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    5713..5969
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6032..7066
                     /locus_tag="PWU81_001083"
     CDS             6032..7066
                     /locus_tag="PWU81_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1109805.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7059..7745
                     /locus_tag="PWU81_001084"
     CDS             7059..7745
                     /locus_tag="PWU81_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1109806.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7769..8617
                     /locus_tag="PWU81_001085"
     CDS             7769..8617
                     /locus_tag="PWU81_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL1109807.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEEKPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            8814..9587
                     /gene="sufC"
                     /locus_tag="PWU81_001086"
     CDS             8814..9587
                     /gene="sufC"
                     /locus_tag="PWU81_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDL1109808.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            9600..10886
                     /gene="sufD"
                     /locus_tag="PWU81_001087"
     CDS             9600..10886
                     /gene="sufD"
                     /locus_tag="PWU81_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDL1109809.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            10883..12118
                     /locus_tag="PWU81_001088"
     CDS             10883..12118
                     /locus_tag="PWU81_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL1109810.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            12105..12575
                     /locus_tag="PWU81_001089"
     CDS             12105..12575
                     /locus_tag="PWU81_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDL1109811.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            12580..13971
                     /gene="sufB"
                     /locus_tag="PWU81_001090"
     CDS             12580..13971
                     /gene="sufB"
                     /locus_tag="PWU81_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDL1109812.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14069..15211)
                     /locus_tag="PWU81_001091"
     CDS             complement(14069..15211)
                     /locus_tag="PWU81_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1109813.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(15262..16059)
                     /locus_tag="PWU81_001092"
     CDS             complement(15262..16059)
                     /locus_tag="PWU81_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1109814.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
     gene            16261..16560
                     /locus_tag="PWU81_001093"
     CDS             16261..16560
                     /locus_tag="PWU81_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1109815.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            16825..17700
                     /locus_tag="PWU81_001094"
     CDS             16825..17700
                     /locus_tag="PWU81_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109816.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            17697..17915
                     /locus_tag="PWU81_001095"
     CDS             17697..17915
                     /locus_tag="PWU81_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109817.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            17902..20529
                     /locus_tag="PWU81_001096"
     CDS             17902..20529
                     /locus_tag="PWU81_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109818.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            20544..20762
                     /locus_tag="PWU81_001097"
     CDS             20544..20762
                     /locus_tag="PWU81_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109819.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            22281..22595
                     /locus_tag="PWU81_001098"
     CDS             22281..22595
                     /locus_tag="PWU81_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109820.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(22648..23421)
                     /locus_tag="PWU81_001099"
     CDS             complement(22648..23421)
                     /locus_tag="PWU81_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109821.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            23526..23771
                     /locus_tag="PWU81_001100"
     CDS             23526..23771
                     /locus_tag="PWU81_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1109822.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            23927..24778
                     /locus_tag="PWU81_001101"
     CDS             23927..24778
                     /locus_tag="PWU81_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HDL1109823.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            24811..25485
                     /locus_tag="PWU81_001102"
                     /pseudo
     CDS             24811..25485
                     /locus_tag="PWU81_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
     gene            25742..25930
                     /locus_tag="PWU81_001103"
                     /pseudo
     CDS             25742..25930
                     /locus_tag="PWU81_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            25976..26941
                     /locus_tag="PWU81_001104"
     CDS             25976..26941
                     /locus_tag="PWU81_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1109824.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            26963..27175
                     /locus_tag="PWU81_001105"
     CDS             26963..27175
                     /locus_tag="PWU81_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDL1109825.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            27250..27726
                     /locus_tag="PWU81_001106"
     CDS             27250..27726
                     /locus_tag="PWU81_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HDL1109826.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            27723..29531
                     /locus_tag="PWU81_001107"
     CDS             27723..29531
                     /locus_tag="PWU81_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDL1109827.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            29608..29823
                     /locus_tag="PWU81_001108"
     CDS             29608..29823
                     /locus_tag="PWU81_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HDL1109828.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            29915..30778
                     /locus_tag="PWU81_001109"
     CDS             29915..30778
                     /locus_tag="PWU81_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109829.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            30815..31351
                     /locus_tag="PWU81_001110"
     CDS             30815..31351
                     /locus_tag="PWU81_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HDL1109830.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            31365..31787
                     /locus_tag="PWU81_001111"
     CDS             31365..31787
                     /locus_tag="PWU81_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HDL1109831.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            31717..34116
                     /locus_tag="PWU81_001112"
     CDS             31717..34116
                     /locus_tag="PWU81_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HDL1109832.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            34148..36139
                     /locus_tag="PWU81_001113"
     CDS             34148..36139
                     /locus_tag="PWU81_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL1109833.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            36162..36413
                     /locus_tag="PWU81_001114"
     CDS             36162..36413
                     /locus_tag="PWU81_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HDL1109834.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            36403..37632
                     /locus_tag="PWU81_001115"
     CDS             36403..37632
                     /locus_tag="PWU81_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HDL1109835.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            37629..39710
                     /locus_tag="PWU81_001116"
     CDS             37629..39710
                     /locus_tag="PWU81_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HDL1109836.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            39859..43779
                     /locus_tag="PWU81_001117"
     CDS             39859..43779
                     /locus_tag="PWU81_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HDL1109837.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            43780..44724
                     /locus_tag="PWU81_001118"
     CDS             43780..44724
                     /locus_tag="PWU81_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109838.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            44728..44931
                     /locus_tag="PWU81_001119"
     CDS             44728..44931
                     /locus_tag="PWU81_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HDL1109839.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(45231..45467)
                     /locus_tag="PWU81_001120"
     CDS             complement(45231..45467)
                     /locus_tag="PWU81_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1109840.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(45650..46036)
                     /locus_tag="PWU81_001121"
     CDS             complement(45650..46036)
                     /locus_tag="PWU81_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HDL1109841.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(46018..47346)
                     /locus_tag="PWU81_001122"
     CDS             complement(46018..47346)
                     /locus_tag="PWU81_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL1109842.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(47307..47636)
                     /gene="mobC"
                     /locus_tag="PWU81_001123"
     CDS             complement(47307..47636)
                     /gene="mobC"
                     /locus_tag="PWU81_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDL1109843.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(47894..48264)
                     /locus_tag="PWU81_001124"
                     /pseudo
     CDS             complement(47894..48264)
                     /locus_tag="PWU81_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            48409..48594
                     /locus_tag="PWU81_001125"
     CDS             48409..48594
                     /locus_tag="PWU81_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL1109844.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            48752..48919
                     /locus_tag="PWU81_001126"
     CDS             48752..48919
                     /locus_tag="PWU81_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109845.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            49537..50196
                     /gene="vanR"
                     /locus_tag="PWU81_001127"
     CDS             49537..50196
                     /gene="vanR"
                     /locus_tag="PWU81_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HDL1109846.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            50199..51560
                     /gene="vanS"
                     /locus_tag="PWU81_001128"
     CDS             50199..51560
                     /gene="vanS"
                     /locus_tag="PWU81_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL1109847.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(51651..52952)
                     /locus_tag="PWU81_001129"
     CDS             complement(51651..52952)
                     /locus_tag="PWU81_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL1109848.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            53231..54037
                     /locus_tag="PWU81_001130"
     CDS             53231..54037
                     /locus_tag="PWU81_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HDL1109849.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            54055..54762
                     /locus_tag="PWU81_001131"
     CDS             54055..54762
                     /locus_tag="PWU81_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDL1109850.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            54759..55730
                     /locus_tag="PWU81_001132"
     CDS             54759..55730
                     /locus_tag="PWU81_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HDL1109851.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            55723..56751
                     /gene="vanB"
                     /locus_tag="PWU81_001133"
     CDS             55723..56751
                     /gene="vanB"
                     /locus_tag="PWU81_001133"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HDL1109852.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            56757..57365
                     /locus_tag="PWU81_001134"
     CDS             56757..57365
                     /locus_tag="PWU81_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HDL1109853.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(57431..57604)
                     /locus_tag="PWU81_001135"
     CDS             complement(57431..57604)
                     /locus_tag="PWU81_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109854.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT        17025 a        12357 c        13801 g        14515 t
ORIGIN      
        1 gttgttgtta gaaggatatc tgcaaaatta aaaacagttg tgattcggac attgttccat
       61 gcgagaatca caactgtttt ttcatgttca ttctaaactt tgatcagcca aataaatagt
      121 aaagccatca ctgtttggag aagcccgaca gctaatccat aagataagaa gcgtgtacct
      181 tctttcatga attttgcaaa gtctaaacgc agaccaattg ccgctaaagc agtgatctca
      241 aaccagctgc tgataaaatg tgccgattgg tcaataacta caggtaaaga aaacaagctg
      301 ttgaccaaac aaaagatcac aaaccctaat acataccaag gaagcggtaa ttttcgtttt
      361 ttctgcgtat cagttggcgt ttcaaacaga gctggttgtt tcttgtgcat gaatttttca
      421 aacagataga caacaacaac gagcatcata atacgtagaa tcttaaatag catggcgaat
      481 tcgacaacag aattgtcaag taagcttgcc ccagcgacta cttgaccaac agattggagt
      541 gtaccaccca aaagtgcact tttttcaatg agatttccac caaacaactg taatccaaga
      601 aaagggagag tcaacatcat cacagtaccc agtagattga ctagtgtgat gatctgtcct
      661 ttttcttcct ctttcgcatg gatggaaggg gcgattgaag caatagcaga agaaccacaa
      721 acagcgtttc ctccagccat catcaaagaa gaagtatcgc tgaattgcag ttttttacca
      781 atcaagtaag caccgatgat agtgactgac atttgcagta acacaaaaac aattcctttt
      841 acaccaattt gagcaatcgt ttgagctgtg acagttgcac ctagtaaaaa aacagagaat
      901 tctaatagtt tgctttcagc aaattttgtg ccgattgcta gaacaggctg tttaaaaaaa
      961 gtatttccta ataaaatacc caataaaata gcaatagtcg ctccacctaa ctgtggcaga
     1021 taaatagcaa gaagcctgct gaaggcagcg atggcaaaag aaagcagtaa accgggtaag
     1081 atttcgcgga ccgctgaatc ctttacagat tttattggat gtgacaatga tttcatttga
     1141 tcggttcctt tcgtcaataa atactaaaag catactcgaa catagtagca ctaaaccagt
     1201 attttgacaa aagaacaaga ggtcgtgaca aaagtcttgt acgacctctt gtttcgaata
     1261 aacggtgttc cagaagtagc ttctacggaa ataagccgaa atttcacaaa aatttgagaa
     1321 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctttta cgacctcttg
     1381 gtaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccagtac aaaaacatga
     1441 acagctgttt ttgtttggcc gttcttatta cttgatgcag aacagctttt tcacaaccta
     1501 ttgaatcgat tattaagcga tttttttaga aacaaatttt ccatgtttgc tatgttcttt
     1561 aattaagaaa cgtacgtaat gagcaagaac gtagaagaca actacacttg caaacagaca
     1621 ttccgcaact tttaagaaat ccaatacgct actatagata cctaaactgt tgaagtaatg
     1681 aacagtagta aatatagctg ttgcagagat aacaagcggg aatgtaaagg ctgcatagct
     1741 tggataaaaa cgaagcttga acaaacgtgg aagcatagac agcacaacga aatacaatac
     1801 ttgagataaa gcgaacaatg ctacgacaag ataaaaatta ggttcagcaa agtttttaag
     1861 ataacctgtt aggcaaagag aaccaggtgc tgcaacgatc gtaatcagcg gacgtgtcgg
     1921 atgagccata tttcttacga aaaagacacg tttgatgatg atcggcaata agaacgcata
     1981 gcagatcaat gaaatccaaa agaaaatttt tccaataagt gggaagaagt tcccggcagt
     2041 tacagtgatg accccaaatc caacgtaaat gataaaccag cttggataaa ttgcttcaat
     2101 tgaaagatca gctttgataa catggtgata attgaaataa aagacaagga tcacttggaa
     2161 gataacagcg cacagccaaa tgaatttgac aattggtgca atactttcat atgaaacaaa
     2221 ataagtacaa ataaccatga gtgacatcgt aaaagttggt gaaacggagg cgatgattgg
     2281 atcatgcaac gtttgtttcg tgtgttcgaa taagataaac aatttaacaa aaatgaatat
     2341 cataagaatc atagagatac cgccagttag attgcctaaa gcaacatggt gatagtcttt
     2401 aaacagattt ccaagggaag ctaacccaag gatcaacccg cagatcggga tcggaataat
     2461 tttcaaaaag ttcttcatat ataatattct cctttttatt taatagattt tagtaccaag
     2521 atgaacgaat acattgtaaa atgaatagga atgtatgatt acgagcaagc agacagctga
     2581 ttgtcttcat ctaagttact agttggatca gcaactgttt tgttgtacat ttctattata
     2641 gatggttttt tgtatattta aatcaaatag tttttatagt aattatcata aaaagttatg
     2701 gaagtggtgc catgtttaag ttattaacga cttttcgagc agtatatgaa acaagaaatt
     2761 tttccagagc agcagaagta ctgtttttat ctcaaccagc agtctcgaat caaatcaaac
     2821 agttggagcg ggaattgaat atcgatcttt ttcaacgtaa cggaagaaaa gagatgcttc
     2881 caactaagca agccgactta ttatatgaac ggacactggt tttgttagaa gaatgggaag
     2941 aaactcagca aaaattgctc aatgaacaaa atgaagaaga agtatgtcgg atcgttgctt
     3001 cccacacctt tgctgtctat cttttgcctc agttgatgaa acatctgatc aaaaggtttc
     3061 ctaaagtgca ttttgagatc gaaatcacga attctcatga atcattagaa caagtagcga
     3121 aacatgaatc cgattttggt tttatcgaaa agccattaga gacttccggc gtacaaagat
     3181 actctttgat ggaagaccag ctagtcatcg ctggggaccc aaaaagcaaa ttgtggcttg
     3241 taagagaagc aaactctggt gtttttcatt acacgcagcg ttattttgaa gagatgaata
     3301 tccaagataa gaagatgatc gtgaaaagta acgatgtaat tgttgcgttg ctgaaggaag
     3361 gaatcggtcg ctcgattttg tctaagcgct ctgttccaga aaatgtgcca atgactccgt
     3421 tgaatttcca tcgatatttt tatttcatcc aacgaaccaa tctggtttct gatcagctgc
     3481 tagagattgc cgaagagatc cttcgataca atgaaagcac agaaacttct cacagcgagg
     3541 agaatctcag ctggtagata attgcatgta tcaaaaaaag ttctatgata cactaaagat
     3601 aagcagtagg cttttgtact gcatttgctc aagcaactgc acaaacggaa agttaatttt
     3661 tcccaaatgt ctatggaaag actttccatt atctaactgg gccgctataa taatcagcag
     3721 tatggttttt aagaaaaggg atgaaacatg gaaaaaaaat taaaagcttc gaacgataat
     3781 cgtattgatt atggcgtgat tttaccagtc tttctgttat gtttgattgg tctgctctca
     3841 ttatatgtag cacttagcca tgatccgaac aatccagcag tcttgaaagg cgtagggatg
     3901 caagttgtct ggtatttagt tggagcggca gcaatcgttg tcatcatgca tttgaattcc
     3961 aaatggatct ggaaactgac tccttacctt tatggtttgg ggttagtggt aatgggactc
     4021 ttgctcaaat tctatgatca gaatttagct gcttcaacag ggtctaaaaa ctggtttcga
     4081 ttcggtgcgt ttactttcca gccagcagag ttgatgaaga ttgcgtacat cttgatgatg
     4141 gcactgatcg taactaaaca taatacgcaa gtaaaagaac ggacgatgaa atcggacttt
     4201 tggctgattg ggaagctctt gatcgtgaca ataccggttt tagcattgat catggcccaa
     4261 gatgactttg gaacgatgct tgtctttctt gcgatctttg gcgggatttt cctgatgtct
     4321 ggaatctctt ggcgcatcat cgcacctgta gtaatcttgg cagtagtcgt tggagcagga
     4381 acgatcttct tggttacgac tgaaggcgga agagacttgc tttataaagt tggattcaaa
     4441 tcttatcagt ttgcacgtat tgattcatgg ctggaccctt tccatgatac gtcaggaatg
     4501 agttatcagc cggcccaagg acttttggca attggtaccg gtggcttgtt tggtaaagga
     4561 ttcaacgtaa gcaatatcta tgtgcctgtt cgtgagtccg atatgatttt taccgttatc
     4621 ggtgaaaact tcggctttat cggtggtgca ttcgtcattt tcttgtattt tattttaatc
     4681 tatcgtatga ttcgggtgtg tttcgatacg aataatgaat tttacgccta tatcgcaagc
     4741 ggtttgatca tgatgctgct gttccatgtc tttgagaata ttggagcaaa tatcggatta
     4801 cttcctttaa cagggatccc attgcctttc attagtcagg gaggatcctc gattttaggg
     4861 aacatgatcg gtatcgggtt gatattatcg atgagatacc aaaatgaagc acctgcaaga
     4921 tcttcaagga ggtaagaaaa ttgtacacct tttactggta cccgaaatgt tcaacatgca
     4981 aaaaagccaa agcgtggtta gaccagcatg gcgttagtta tcaaaccgtt gatatgatca
     5041 aagaagtacc gaaggcctca acgattgaga cttggctaga aacgagtcaa gtaccaatcc
     5101 gtcgcctgtt caacacaagc gggatgaaat atcgggaact tggactgaaa gatcaagtag
     5161 gcgagtttac ggcttcaaaa gcgagccaag tcttatcgac agatggtatg ctgatcaagc
     5221 gaccactgat cttacgagag gaccagcttg ttgcaattgg attcaatgaa aaaatttatg
     5281 aaggagaact gatataaaat ggcaggcaag catttgaaga aaaaggacaa cctttggatc
     5341 ttatttaatg gaaaagaata ctgtgttggt ttgacaaatg aagcgcaaga agaacttgga
     5401 gacatcacgt ttgccaatct gccaaaagca ggacaatcgc taaaaaaaga tgaacctctg
     5461 atcgaagttg aagcggaaaa agcggtcaat gaatttgcca gcccgctaac gggtacagtt
     5521 tcttctgtta atgataaaat cggtgaagat atcaatatct tgaatgattc agatgaaatg
     5581 aatgcgtgga tcttaagttt caaagatgtt gatccaaaag aatttgacga attataaaat
     5641 cagaaaatca cttgaaaaaa gttgacactg attcttccag ttgctataat cataagcaat
     5701 cctatatgaa acgagaaagc tttgaaaaga agagtacatg tcacggacgt ttgaagagag
     5761 ccctgtttgc tgaaaatggg taacgaaaat gagatggaag atggtcttag agcagagtaa
     5821 gcgagcgtaa gcaaacttac tacgggagtg cccgttacag cgcgacagta tacggaaaga
     5881 agctaagtcc aagtactgga agaggctgtt attgcgaagt aacggtaaat caaggtggta
     5941 acacggaagt tcaagctttc gtccttttgc ttaaggcaag agggcgaagg cttttttgtt
     6001 tgaaaaatta taagagaaag atgtgaatgg catgtcatta atccaactaa cgaatgtcaa
     6061 aaagacattc aatgggaaaa acggaaacat ccaagccgtc aatgacgttt cactaaacgt
     6121 tgaaaaaggg gatatttatg gaatcgtcgg ctattctggc gcaggaaaaa gtacattagt
     6181 ccgtctcctg aatggacttg agcttcccac gagcggcgaa gtgatcgtaa acgatcaaga
     6241 tattacaaaa ttaaaaaaca aagaattacg tacattccga aagaaaatcg gtatgatctt
     6301 ccagcatttc aacctgttat ggtcaagaac tgtacttgaa aatatccagc tgccgctgga
     6361 attagctggc gtaccaaaga gcaaacgtaa agaacgggca gaagaactgt tgcgtttagt
     6421 cgggttagaa ggacgcggac aagcatatcc ttcacaactt tccggcggac agaaacaacg
     6481 cgtcggtatc gcacgagcat tggcaaatga tcctgagatc ctgctttgtg atgaggctac
     6541 tagcgcattg gacccgcaaa caacagaaga agtattagac ttattgttag cgatcaataa
     6601 gaaattgaat ttgaccatcg tcttgatcac acatgaaatg aatgtgatcc gcaaaatctg
     6661 caacaaagtc gctgtgatgg agttggggaa agtcgtagaa gaaggagacg tattgactgt
     6721 cttcagacaa ccgaaaaaag aagtaaccaa acgctttgta caacaagaca tcgaaccaga
     6781 agctgattcg acagaactat tagctgaact catcaaagaa aatccaagcg gccgtttggc
     6841 aactttgacc tttagcgaaa gaaatgccaa cgagccagtc atttcccaag cgattcgacg
     6901 ctataacatc gatgtcaacg ttgtgtatgg aaagatcaaa caagtgaaag aaggttcctt
     6961 tggttcattg actgtcttga tgactggtga aaccaaagaa ttagatcaag cagaagtgtt
     7021 catcaaagaa caaggagtag gaattgaggt gatccatcgt ggataaaagc ttttcagaaa
     7081 catatctaga ttttaaacaa atcaacccgg aaacgatgaa tcaggctatc cttgatacac
     7141 tatatatgac attaatctca atggtcttag tgacgatcat cggattagtt ttgggattga
     7201 tcctttactc tattggacgc aaaaacaaac cgtatgctcg aatcatttat ggaatcgtat
     7261 cgatcatcag taatattttc cggtcaacgc catttatgat cctgatggtt ttgatcattc
     7321 catttactaa agcaatcgta ggcactatgc taggtgcgca agcagccatt ccagctcttg
     7381 ttttatccgc agcaccattt tatgcccgtc tagtagagat tgcttttcgt gaagtcagcc
     7441 ctggtgtact ggaagcagca gatgcaatgg gagcaagtta ctgggaaatc atctggaagg
     7501 tcttgatacc tgaaagtatt cccgcattga tttctggatt aacagttaca acgatctcga
     7561 tgatcggatt tactgcgatg gccggcgcga tcggcgcagg tggacttgga ggtcttgctt
     7621 ggcaggaagg ctaccagcgt ggtaatctaa cagtgacatt cgttgcgact ttaattattt
     7681 tactaatcgt atttatcgtt caaggaatcg gtgatttctt aactaagaaa acagacaaaa
     7741 gataatagga tataggggga tgtaggatat gaaaaagaaa atttttggat ttgcagcagc
     7801 gcttttgctg actgtaggat tagcagcatg cggtaacaac agcgacagca aagattcagc
     7861 aaataaagca gacgacacga cattgaaagt cggcgcttca ccgacaccgc acgcagagat
     7921 tttggaacat gtgaaaccat tattgaaaga tgaaggaatc gatcttgaaa tcgtcaaatt
     7981 cgatgactat gtattaccga accaatcttt ggaagaaggc gacattgatg ccaattactt
     8041 ccagcatatt ccttatttga acaaagaaat caaagaaaaa ggatatgact ttgtcaatgc
     8101 cggtgctgtc cacatcgaac caatgggctt gtactctaaa aaagtcaaag atgtttccga
     8161 attaaaagat ggtgcaacaa tcattacttc aaattctgaa tcagactggg gacgcatcat
     8221 cacaattcta caagatgctg gcttagtcaa agtcaaagat ggagtagatc tggaaacagc
     8281 aacattcgat gatattgaag aaaaacctaa gaacttgaag ttcaaccata cgatcgaccc
     8341 atcactttta gcttctacgt accaaaatga tgaaggtgac ctagttgcaa tcaacgcaaa
     8401 cttcgcttac ggcgcagggt tgaatccatt agaagatgcc gtattgcttg aaaaggacaa
     8461 ctctccttac gtgaatatta ttgcgactcg taaaggtgat gaagacagtg acaaaatcaa
     8521 aaaactgatt gaagtgcttc accgcaaaga agtccaagaa tggatcacag acaaatgggg
     8581 cggctctgtt aaaccagtaa aggctgatgc aaaataaggt atggaacaaa aaagatgaga
     8641 aataagtctg taatggctta tttctcatct tttttatacc tattctaatt tagaaatcaa
     8701 aattgtttaa taataaataa aagatatttg attttagtaa ggaaggtcat tgcgcttcat
     8761 tctcaattag tctaaaatga gtgagaacga aagagaattt gggggtataa tgcatgtctg
     8821 tcttagaaat aaaaaatcta catgtgtcga tcgaggaaaa agaaatcctt aaaggcgtaa
     8881 acctaacaat gaaaaccgga gaaatccatg ccatcatggg acctaacggc acaggaaaat
     8941 ctaccttatc tgccgctatc atgggcaatc caaactatga agtaacagaa ggcgaaattt
     9001 tgtttgacgg gaaaaatgtt ttagacttgg aagtagatga acgtgcgcga cttggcttat
     9061 ttttagctat gcaatatcca agtgagatac caggtatcac gaatgcggaa ttcatgcgtg
     9121 cagcgatcaa tgcgaaacgt gatgaaaacg acaaaatttc tgttatggca tttttgaaaa
     9181 agttagatga aaaaatggct ttactaaata tgccagaaga aatggctgaa cgttatctga
     9241 acgaaggttt ttctggcgga gaaaagaaac ggaatgagat cctgcaattg ttgatgttag
     9301 aacctacatt tgcaatttta gacgaaatcg attcaggtct ggatatcgat gctttgaaag
     9361 tcgttgctaa aggagtaaat gaaatgcgcg gagacaattt cggtgcattg atcatcaccc
     9421 actatcaacg attattgaac tatatcacac ctgatgtcgt tcacatcatg atggaaggcc
     9481 gagtagtcat gacagggaat gctgacctag ctaaacgtct ggaagcagaa ggctacgctg
     9541 gtatcagtaa agaattagga atcgactata aagaagaaga agcgtaaggg ggagcaagaa
     9601 tgaaagaaaa aaactggctc gattatcttg acgcggtaaa tgatttttct ttaagtaaag
     9661 gcgaacctga ttggatgaga accttccgtc aagacgcact agcgaaagca gatgaactgc
     9721 cattgccgca tatcgatcgg gtgaaattcc atcgctggtc gttatttgat gtgaaagaaa
     9781 cacaaaccat cagtgaaaca ggcacgattc cggcatttga tgcaatgaaa gacaatcctg
     9841 tgcttgtcca acaaggttca tggacgattt ttgaacaact gcctgtggaa ttagcagaaa
     9901 aaggagtcat ttttaccgac ttgttcactg caatgatcga atatccagaa ctagtccaag
     9961 aatattatat gaaaaaagca gtaaacatga acgaagacca attgactgca ctccatgtag
    10021 cttttatgaa cagcggtatt ttcctgtatg tgcctaaaaa tgtcgtgatc gatgaaccac
    10081 tcgagtcctt attcattcaa gatggcgcat ccgatgagca cttcttcaag catgtgttga
    10141 tcgtggcaga tgaacatagt gaattttctt atctggaacg tttccagaca accaaagaac
    10201 aagtagcaaa atcatcaggt aatatcatcg tggaagtcat cgctaaagct ggttcaaaaa
    10261 tcaaatattc agccgtagat caattaggag aaaatattac ttcttatatg aatcgccgtg
    10321 ggcacatttt acgagatgct tctgtcgact gggcaattgg tgtcatgaat gacggtcatg
    10381 tgatcgctga ctttgactca gatttagcag gagaaggtgc acacgcagaa gtcaaaatcg
    10441 tggcaatcag ctcgggcaga caaatccaag gaatcgatac acgagtaacc aataaagccc
    10501 ctcatacgat cgggcatatt ttgcaacacg gagtgatccg agaaaaagga acattgacct
    10561 ttaatggtat cggccacatc ttgaaagggg caaaaggtgc agatgcccaa caagaaagtc
    10621 gtgtcttgat gctttctgat aaagcacgtg gtgatgcgaa tcctattctt ttgatcgatg
    10681 aaaacgaagt aactgctgga catgctgcca gtgtaggacg agttgatcca gaagaaatgt
    10741 actatctgat gagtagagga ttgcataaag aagaagcaga gcgcttagtg attcgaggat
    10801 tcctcggttc tgtcttaaca gcaatccctg tagaacaagt tcggaaagaa ttagtcgaag
    10861 taatcgaagg gaagttgaat ggatgatcga tgctaaaaag ctacgcaatg attttcctat
    10921 cttatttcaa gacgtcaatg atgaaccttt agtttatctg gataatgcag cgacgaccca
    10981 aaagccaaaa caagtactcc aggcattagc gtactattac gaacacgata acgccaatgt
    11041 tcatcgcggt gtccatacgt tagcagaacg cgcgacccat gattatgaac aagcaagaga
    11101 aaaagtacgg gcgttcatcc atgcaaaaga aacagcagaa gtgctgttta ctcgtggtac
    11161 gacgacaagc ttgaattggg tagccagaac cttcggcgac cgctatatca aagcaggaga
    11221 cgagatagtg ctctcttaca tggaacatca ttctaatgtt atcccgtggc agcagttggc
    11281 aaaacgaaca ggtgctgtct tgaaatatat cgaactaaca gaagaaggct tcctggatat
    11341 ggaggatgca gcgaaaaaga ttacggacaa aaccaaaatc gtctccattg ctcatgtatc
    11401 caatgtctta ggtgtcttca atcctgttca agaattagct gaacttgtcc atgaaaacgg
    11461 cggggtcatc gtagtagatg gtgctcaatc tgccccgcac atggccattg atgtccaagc
    11521 actagactgt gatttctttg ctttcagcgg acacaaaatg tgcggaccaa caggtattgg
    11581 tgttttatat ggtaaacgaa aatggttaga agaaatggaa cctgttgaat ttggtggcga
    11641 aatgatcgat tttgtcgaat tatacgacag tacatggaaa gaactacctt ggaaattcga
    11701 agcagggaca ccaaatatcg ccggcgctat cgcattaggc catgcaatcg attatttgca
    11761 aaaactcggt atggacaata tccatcgata tgaagaagaa ctagccgctt atgtgttgcc
    11821 aaaattacaa gcaattgatg gattgactgt ttatggtcca caagacccga attttcggac
    11881 aggagtcgtt gcattcaatt tggatgggtt acatccccat gatgtcgcga cagctttaga
    11941 catggaaggg gtagccgtca gagcaggtca tcattgtgcg caaccgctat tgaagtacct
    12001 tgaagtatca gcgactgctc gcgcgagctt ttatttctac aacacaacgc aagacgccga
    12061 ccgtttagtt gaagcaatca tggcaacaaa ggagtttttc caacatggga ctatctaaat
    12121 tagacaattt ataccgtcaa gtgatcttgg atcattcttc ccatccgcat catcgtggaa
    12181 gcttgactga ctcgacaagc aagatcgaat tgaacaaccc cacatgtgga gatgtgatcg
    12241 aattgcaagt cgatgtaaaa gacggaatca tcaaaaatat cgcctttgac ggcagtggct
    12301 gctccatcag tacggcaagt gcctcgatga tgacagacgc cgtcatcgga aaatcaatag
    12361 aagaagcaga acatttaact gtagactttc tacagcttgt ccaaggaaaa gaagtcaaag
    12421 atcccgacca attaggcgat gctgctatgc ttggtggtgt agctaaattt cccgctcgga
    12481 tcaaatgtgc gacacttgcc tggaaagcac taggaaaagc aatggaaaac gatggtaccg
    12541 cagctttcca tcattaccac aaggaagagg agtgagaaca tgggcgtacc agaattagaa
    12601 gaatataaat tcgggtttca cgatgatgtc gaaccggttt tcagtacagg agaaggcttg
    12661 acagaagaag tgatccgtga gatgtcaaga atcaaaggcg aaccagaatg gatgcttgaa
    12721 ttccgtctga aatctctaga aacatttaac aaaatgccaa tgcaaacatg gggccccgac
    12781 ctttcagaca tcgatttcga tgccatcaaa tattaccaaa aaccaagtga caaacccgct
    12841 cgtgactggg aagacgtacc agaaaaaatc aaagatacat ttgaaaagat tggtatacca
    12901 gaagctgaac gagcttatct agccggtgct tccgcacaat atgaatcaga agtcgtgtac
    12961 cataatatga aagatgaatt cgaaaagtta ggaatcgtct ttactgacac tgactctgct
    13021 ttgaaagaat accctgaatt attcaaagag tacttttcaa aacttgtacc gccaaccgac
    13081 aacaaattag cagcgctgaa ttcagccgta tggtcaggtg ggacctttat ttacgtgcca
    13141 aaaggcgtca aagtcgatgt tccattacaa acgtacttcc ggatcaatgc ggaaaacacg
    13201 gggcaattcg aacgtacgct gatcattgtt gacgaaggag caagcatcca ctatgtcgaa
    13261 ggttgtacgg cacctactta ttcaagtaac agcctgcatg ccgccatcgt agaaatcttt
    13321 acaagaaaag atgcgtattg ccgttacaca acgatccaaa actggtcaga caatgtgtac
    13381 aacttagtaa ccaaacgagc aaaagcatac gaaggcgcaa ccgttgaatg gatcgacgga
    13441 aacctaggtg caaaaacaac gatgaaatac ccaagtgttt acttagatgg aaaaggtgca
    13501 cgaggcacca tgctctccat cgcctttgca ggagcaaacc aaatccaaga tacgggtgca
    13561 aaaatgatcc acaatgcacc aaatacatct agctctatcg tctctaaatc aatttcaaaa
    13621 gacggcggag aagtaaacta tcgaggacaa gtaacctttg ccaaaaacag tgcgggttcg
    13681 atctctcata tcgaatgtga cacgatcatc atggacgaat ggtcgaaatc cgatacgatc
    13741 ccattcaatg agatccacaa cagccaagtc tctttagaac acgaagccaa agtctcaaaa
    13801 atctcagaag aacaactcta ctatctgatg agccgaggcc tgaccgaatc tgaagcaact
    13861 gaaatgatcg tcatgggctt tgttgaacca ttcaccaaag aacttccaat ggaatatgca
    13921 gtcgaactca atcgattgat cagttatgag atggaagggt ctgtaggtta ataactattt
    13981 cattgctcga aacgttgata ttaaagcgtt tcgagcgttt ttttatttgt attttaatgg
    14041 ttggctacgt ttttggctac gtttggaatt attcgcccat aaattttgca aatcggtcac
    14101 cagttttctt aacagattgt ggtgtgacat gagcatagat attcatggta gttttaatat
    14161 ctttatgacc taatcgttcc tgtacttctt ttatcgacgc accactttca aacaataagc
    14221 tacagtgggt atgcctaaat ccatgtggag taatttgagg ctccatcggt gtctttttgt
    14281 aaatccagtc taaccaatcg tttactactt gggggtagta caacttgttt gtgtttgtag
    14341 tgaaaagaaa ctgttttggt gaattcgtgt tatagccata taagatcatt attttcattt
    14401 gttcttgttg ccaaatacgc agttgtttaa gtgtaaaatc atcaagctga atcgttcttg
    14461 atgaattttt agttttaggt acttgtagaa ctacttgatt aaactcgtct tgagcgatag
    14521 ttttaccaat tgttattgtt ttattgaaat agtctatatc ttcccattga agcgccaatg
    14581 cttcgctttt acgcattcct gtataagcta ataaacggaa aaaagtgtac agtttaactg
    14641 aattattttc tttagtattc tgtaaaaaca gttgtaattg atctttcgtg taaaacttca
    14701 agggttgttc ttcttcaatt tttcttggaa gcaatgtttt agacatagga ttatccttac
    14761 agacttccat tgctactcca aatttaaata tttgagcagt tgtttttctc aagaaaccat
    14821 attgttttaa tgttttgtgc cattcattca cctgtttttg gcaatagcta acacttattt
    14881 tatccaatcg taattcccca aaagcaggta atatatggtt tttgcaaaaa cgttgagcaa
    14941 cggcaacaga agacggtttt actcttgttc tgtagttatc aatccataat tcatacaact
    15001 cttgaaaagt tgtaacattg attttaaact cttgtttttc tttaaagtct acaagtagtc
    15061 tattaagtgc tgtttgcgcc tcagcttttg atttgaatct tctacgcttt gtacgtatat
    15121 cttttccagt cttgggatct ttccctaaat aagtttcaaa tccccaatat ttttcgccat
    15181 tttgtaattt ataactaaaa aatgttgcca tttgattctc tccttacttc attattgcag
    15241 gtagagaagt tatttgaaga atcaattatt tttaaattta tcgtgattat cagttaaaaa
    15301 ttttgcgatt atactatttt tctcatatgg gaaattgtat tttttactaa gatctgaaat
    15361 aaatttttct gtttcaaatt ggtttttagt aattttataa aagaaatcct cgacagatga
    15421 atactttgaa atatctttat aggctaagtc catttcgtct aatagctgtt ttaaagaatc
    15481 aatcattctt tctttttcac cgtatttgaa atcaccattt cttatcttgt cttgaagttt
    15541 actgttggct cttttgaaaa atagctcttt agagatagag tgtctttggt tagcgtactc
    15601 ttcaatcaga ggattgatat tattagcaag tatctcattt agataaattt caaaatcttt
    15661 tgagtaatat tttgtaaata tattttttat tataggattc aataactcat agtctttatc
    15721 tagcgaaaac gtatcactat atctttcttg aatgtcttta aagactttgt gaggtatttc
    15781 tgggaagtcc ttaatatata gctcataccc tatatcgata aggtatgaag ttatatattc
    15841 ttctaaagtt ccccatttaa tccagtcaat tgttgaatta gtgatagttg ataataattt
    15901 cagcttttgt ggatttggta aattggtccc acgttcccag ttggatatgg tcgcacgtgg
    15961 tgaattatct attttccttc caagttcttc catagtaaaa ccacatgaaa ggcgtaattg
    16021 tttgattcgt tttcctgctt ctgaaggatt tggcttcatc ttttttctcc tttttatttt
    16081 taaagatagt atatcatatt tataaaataa aagtatttaa aagtatttac tttttatctg
    16141 gagtatgata ctttattctt ataaatacaa ataaatacat ttttttacgc aatttgtatt
    16201 tatttgtact tataaaggta gaggagttat agaataatta attaaaaagg agaggttttt
    16261 gtggaagcaa taaaattaat gatttcagaa gaacaggagg aaatgtttct aaagcagttt
    16321 tatcaaattg ctacaaaagc tattgaaaag gctcaagaaa atgcggggat gacaagggaa
    16381 tatctcaatc aaaaagaaat ggcggaatac ttaaatgtga gtgaaaactt tttaaaaaat
    16441 gctgtgagaa atgagggatt gcccgtagtt gaattagggg caagaaaatt ttattcgaaa
    16501 aaggcagtta atgagtttat gtttgctcga atgaaaagag gggaaaatat tggaagatga
    16561 tgaagtagaa attataaaaa aatgctttaa aaaaagaaaa aaagtgttcc caaaagtttg
    16621 cagactgggg gaacactcaa taataaagtg ctgagaaaac tttatttata gctattatat
    16681 caaaaaagaa ggtgaaaagg tgttttcttc tggaaatgtt gtagttgaca aacttagaga
    16741 aataaatttt gacggaaatg taatccacac acatggtacg atcaattaag aaaaaaaacg
    16801 aaaaaaggtg ttgagaagcc ctatttgaat gcaatagtta tcttagcaga aattacctat
    16861 tggtatagac ctaaaaaaat ttttaacgat gatggtcagc tacttggtta tcaaaaaaag
    16921 tttcttgaag atattttgca aaaatcatat aagcaattaa gcaaaaaaac aggattatca
    16981 gaacgagcta taaaagatgc aattgtgtac ttagagaaaa aggggattat caaaagggtt
    17041 tttagaaata tttattatga gaaaagagca gttacattat caaatgttat gtacattgtt
    17101 ttaaatgtac ataaattgat agaaattact tttcctactt tggattgcga aaaaaaggag
    17161 tatatgccga aaattcgtca gaggggtatc gaaaaaaaga gatatgtctc tttaaaaaaa
    17221 ggtagaggta tgtacaaatt tgtatatacg tatacaaaga ctacttcaca gaacatatca
    17281 aagactactc tcgttatgga agaagaggat attaatataa agaggattta cagtaagatt
    17341 agtgttcttt atgacgcaaa taacgaagat ggtcgaaaat tatttcaagt gtatcactat
    17401 ttgagaaacg ctaatgtaga tacagaagat atcagaaaaa tcattgaata tttatctgaa
    17461 aatactcagt atcttattgc tgagtgtatt ataaaacaac atgaagcatg cttttttcaa
    17521 agaaaaactg aagaagggtt atacgattat gtaacatatt ttttgaatgg acttcagaaa
    17581 agatataaaa gtcaattttt agaaaatgta gagcctatag atcagttcaa aaaagctttc
    17641 gaaatcaaag agttacctga aattactatg cataattggt tgaaaggaga agaatcatga
    17701 ttaaaaatcg cttaatagat caaatgtggt gtcttgatca aaatacagat actctcgaat
    17761 taatcgagag tatctgcttc aacacagtag ttctcgattt aagagctgaa aatgacaatt
    17821 gtgtaactca tatcataatt aatcaaaaaa tggctcagca gctctctgag agcctaagaa
    17881 aatgggctga aggagaaaac tatgaaagca attaaagaaa aaatcgttag tagaaaaata
    17941 aactggttag cactcagtgt acttgtttta atgattagcg ttgtttttac aagtaatatt
    18001 gttgaagcta gcattttcaa ttggtttgat gacacaaaaa gtcaaacaga gtttttgaat
    18061 aattctcttt atagacctta cctaaaaaaa tacaatggag atttccttag tcagttcggg
    18121 atttggcttg gatgggctgt ggtaaaaggc atgtttactg tgactgattc gattcaaaat
    18181 atgattccag atgtgttgga tttgttcaat ttcattgaaa gtacagggct aaataatgtt
    18241 tatcaatcag tcataaatac aattgtcgtt ggattaatga tcctttcatt gatgtttgtt
    18301 ggctacaaga tgattactgg aaaaggaaca atagatttaa gatctgttgg aatgaatata
    18361 gtgatgtctg tagcgttaat tcttttaatg ccaacaatga tttctagcgg tattcaattt
    18421 gccaaaatat tttacaatga tgctactacg attacaaatt cagatgacgg agtcgcttgg
    18481 tcattgataa aacaaggagt aacagatctg gcatatatta ataaaacaga tcaatactca
    18541 agcattgata aaacggagga tcgaaataag ttaacacgaa aaaattttca acagacagat
    18601 ttaacacaag tgttgacgga taaagtgatt gataaattgg aaaaagaaaa tccagctgcg
    18661 gataatcttc gctatgaatt agttgaaaat tctaataatg aatttgttgc cacaaagttt
    18721 tctgataact tcttatcaac attttctgat agcttgaaat caggttatta tcgatatcaa
    18781 gcaaatcttt gggggatttc gattgggttg acagcgttgg caatcgccta tgtgttcagc
    18841 gcgtttgtga tcattacagc aatattagaa cttgcattta aacgtgtgtt aggtgttctt
    18901 gtttttgcga cagatattga aacaggacaa cgttcaaaag ttgttttatc cgatatttta
    18961 caatgttact taacagttgg atttcaaggg tttggactat ctatgtttgc tatgtttatc
    19021 aattttttaa atgctggaca aggtatctcc acaaatattt ttataaaaac aattgcttat
    19081 atctgtgctg tgtttgtgct gataaaaggc agtggaacag tcatgagata ctttggtgtt
    19141 gatattggac tcaaagaagg ctatgggcaa cttgcttctg cttttggtat gggcgcaatg
    19201 ctgtttagaa aaggatcaac tggatttaat cgtgctaaag gaagcggaaa tggaaatgga
    19261 tcagacagtg gagaaggaga agatcgaaaa ccagaaaaga atttcggcga aactctatca
    19321 aaaaaagctg gcaaaactgg aagagctttg ggatatgccc atgaacgtgg attatcaggt
    19381 ttagcttcag atggagctac aatggctagt gaaagagcaa taaaaccatt taagagtatg
    19441 cgtgatatgg ctaatgatac caagaataag ttcaaagaag gtttagatga tggtacagtg
    19501 tccgctatta ataaaaatag caaacctatg ttagctaaaa ataaagaaga tgaaactgga
    19561 aaatatgctg attccatgcc aacacgtctt tcggatagaa aagatggtgc taaggttgaa
    19621 aatgccgata gaattatgtc tagttctgaa cgtatgcgtg aagcaatgaa aaataatgct
    19681 gaatcgaata ataatcctgt aagtgcaatt cagcaaaaag ttcaacaaga tattgaagaa
    19741 cgtaaaaatg ctatgcacgg tcaagctaag tctgctgaag aattaattaa tcagaagcga
    19801 caagaagcta aatacacgcc tgaagcaatg aatcgtgagg aaatgttaag gaaacgtgta
    19861 gaagggcgta caggagctaa tacggacgaa aaagaagcgt tgaccaaaga aagaatccaa
    19921 gaagctaaaa actcaaatac cggattagaa aaattggtca aagaaaatct acaaaattct
    19981 acaagtagtc aaggtggccg atcagtagat gtccgagaaa atattcaagg ttcattgaat
    20041 ggtcatgtcg gacggaatgt tgatgtaaga gagaatttac aaagctcttc aaatggtcaa
    20101 ggtactaaga caattgatgt tcgagaaaat gtacaaaatt ctacaagtag tcaagggttt
    20161 aaatcaatgg atgtaagaga gaatgtacaa aattctacaa gtagtcaagg gtttaaatca
    20221 atggatgtaa gagagaatgt acaaagttct acaagtagtc aaggattcaa aacagtagat
    20281 gttcgggaga acgttcaaaa agatagtgga gaaatgaaag aaaaaacaca aaaaatcaat
    20341 atcgttgaag acagaaagtc agcgaaaaaa tttgattcaa atcatgaaac agtgattatt
    20401 gatagtgaaa tccgtgaaaa tgataaggga tcaaaaccaa gacgaagatt tacctatgaa
    20461 gacaacgagt tattcagaga tacacttaat gatcctaatc cgcttttcga taatctatta
    20521 aaaaaataaa agggggaatt gtcatgaaat taattctaaa gattagtacg catgatttct
    20581 tagaacttga cgaattatgt gaatcagtaa agaacgcaac aaatgaaaaa atcacggtca
    20641 ctaattatat tgaactgatg attcaagaag aattaaagat gaaacatgag ataaaaaaca
    20701 acttgaaata ttgttatcta gaaaaaagtg aagagataat ccatgaatta aatggctttt
    20761 aaaaattgta tttgacatga atttacgaca aggaattagt ggggattaag cgattattct
    20821 cttccacttt agagaaataa tgggttaaaa agccagaata cgtagagcaa tattgacaac
    20881 ctataaagga gctattgttg aaaattgaag taacaaaaag ataaagggag gtttacatct
    20941 atcagattaa aagtgatagt acacccatga aacctgcaaa agagttgaac ctaatgtata
    21001 tcggtttaac tctttttatt tgcaaattaa aaaaatatca ccgaagtgac gaatgaaact
    21061 atgaccaaat taagcaagaa aatatatctt gcaaataaag tagtataaaa agatattgat
    21121 aaagccaatc aaaactttga atttcaaacg ttagataatc aaacagatac aaaaaatgaa
    21181 atcagttcta cgcgtacgat tattaaaaat aataaatttt cactagaaga aaatggaaat
    21241 aaaatcaaat aattaggaag aaaaaacaaa gcaattagaa aagcctgcaa gcaaatttga
    21301 gtaatcgaga tccaattttt ttaaggaagt ttaacaaaaa tatgtaattc tattatcact
    21361 attaaagaga aagttggagc ggaatataaa gaaaagagaa aagtaattaa acgcaaaaaa
    21421 atgtagaact taaaaaatat aaagaggaac aaccgttaaa attaggaatg atgttgtgta
    21481 aacattataa ttgctatgat ataaatgaag tgttaaagaa agcaaattta aaccaatttt
    21541 gtgaacagaa aagccttagt cactatgacc aaaactacgg acgaaactat gaccaaaagt
    21601 aggtactaaa ttatgaccga aaataggtcc aaaatggatt tttttatgat atcaaaaatt
    21661 atgaaatgat tttgaaagaa aaccagttgg cacagccaac ttagccacct tcctgtagga
    21721 aggtgctatc agttttccct tatgctcttt tctttctttt ttgtttcata tgtcaaatgc
    21781 cattttgaaa tgtaccatct taagaaattt atccttaaaa acaattgcgt tattggataa
    21841 aaagatgcta cattatctta aaaaaggaat caaatatgat gaatttagtg gctagcatat
    21901 cgtgtgatta agatacttta tagtgtaagt tgaaggagaa aaaatattta aaatatactt
    21961 cccatttcag tgggaagtaa aattaatctt gttgtttcta ctagtaatgt atctttttgg
    22021 aaactatgta acgatgtaaa caagattttt ttcagaagaa aaaacgataa aagttgtgat
    22081 aaacgattta aaaaatagga gaaaatatat caagcgcatg gtatgaaacg cacatttaaa
    22141 aagattcgac taactaaaaa aataagttta caaagcattg ttgtgtatta caagtgatct
    22201 atacactgaa aaatgataga gaaaatctga tacgtccgca acaatggaca attgaaaatg
    22261 aaagtgagga aattggaaga atggctgatg aaaataaagt tgttccgctt gaaacactag
    22321 tgataaataa ggaagagttt gagcaacttt ttcttgattt ttttgttcaa tatgacagga
    22381 tttatgattt tagttcgtca ctagatttat ttgaattaga ggcagaaata atagaaaaca
    22441 gtcgagagga gaatggtaaa atcatcattt cagtagaaaa attcaatgaa ttattctcag
    22501 aaacatttga aatagataca gattattatc aaccacaaga agttttccaa tcatttagaa
    22561 atcagattat tcgctcaggt aagaccgtaa actgaaaaac aataaaagaa acgttgaaag
    22621 aataattatt ccttcaacgt ttctttttta gaatgtttta atgaccaaag cagtttcatt
    22681 gtcaggctta ggtaattcat gaaatcccgt atttatagtg ctcaattgat caactaacca
    22741 tgttttactt tttgaattgt agataaatct aaaattaata tcgttattat cgtgttgaag
    22801 gggcgtctcc aaataatctg ctgtatattc atagtcaacg taacctgcta atacaaaaga
    22861 gagtatccct ttatttaatg ttagatttaa tggataatca tacgacttta tttttttcaa
    22921 cttcatattg tacatatctt cagccataaa agttgagttc gtgatttcat cttgggtctc
    22981 attaaaaaag ttatccgaat aatcgcttat ttttgaaaag tctttattgg caccagcctc
    23041 aaaaaattgt tcaacttttt cagtataaag ttgtgctaac gctccttttt caatattaac
    23101 tttaatattt tcatgtctat caatttgtgt gctgttcagt aagggaagat cagcttttag
    23161 ttttatatcg tcttgatttc tttcaatttc aattggcaaa atcttttcag tagattcagt
    23221 ttctgataaa ggaacatcta gttttttatc atgataataa gcttcaaatc catctggaac
    23281 cggaaaaaaa tgtattgggt aattcttttc tgaggttgtt gtactagttt ccgtacttgt
    23341 agaacttgtg tcagaagtgg tacacccgct tatagttccg atgagtaaag cgcaagttag
    23401 aatgattgca aatttcttca aaaaaattcc tccttaaaat tgaatatgga ctaatactag
    23461 tcccattgta gcataccgaa tctttaagat taataagaaa aaggactaat tttagtccaa
    23521 aaggtgtgct attcttgaaa gaggaaaaaa ttattgataa agaaaatgta atcggtagca
    23581 atattagacg agtccgatta gaaaaaggaa ttggtcaaac cgagctgatt agagacctac
    23641 agcttcgtaa aattgctata actagggaaa cgttggttaa gattgaaggt ggccgccaac
    23701 acattaagtt ggatcaattg aaagccatca aagatattct acaggtgtct tatgaagatt
    23761 tgcttcaatg actacttgta gtcatttcgt ttgaaattat tatgttacac ccttatgtgt
    23821 cacgaagaaa tactggtata ataatgaatg ggatatgtaa cgatagggta tactgttatg
    23881 ttgcctaata aattgtggat caaaatacaa gaatggaggg atatccttgt ataaggcata
    23941 tgaattggat tttacaaatg ctgacttaaa tgcattctcc tcaagctcgc aatcatatac
    24001 acatgtagta aatcaaatag atcacaacca aaaatctatt aacaagagaa tagaaaatct
    24061 atttgaaagt gaaaatgatt tagttgatta ttattctaag gacagcaaaa ttctagatgc
    24121 agacgaaatt attaaagatt ggtttccaac tataaaagca gatattttta tatctcactc
    24181 tcatgctgat gaaaaattag ctatacgttt tgcatcatgg ctatttgaaa attttggtct
    24241 tactgcattt attgattctt cggtttgggg atacagttct gatttattaa aaaaaataga
    24301 ccaaaaatat tgctataagg aacaaacaaa aacatatgat tatgataaaa gaaatgtaac
    24361 aactagtcat gttcatatga tgctttctac agctttaaat aatatgattg atagtacaga
    24421 atgtctgttt tttcttaata cacctaattc aattagtttg agcaatgaaa tcactaatga
    24481 acagaaattt acgtattcgc catggctata tagcgaactg acgacagctt caatagtgga
    24541 aaaaaagaat ccacgattgg aaagtaatcc tcaaatgagc accgaggatg ttagaagcat
    24601 tataaagcac tattcttcgc aagaaaatct gcaaataaaa tattctttaa atttaagtga
    24661 attaacttat ttacgaaaag ctgatttgaa aaaatgggct ttagatgctg tggaatgcac
    24721 taaaggaagt aatacattag atattttata taaaaacacc attttcacta atctctaatc
    24781 aatttactga atcgaaagga accgggtaga atggataaga aagttttaat aaagaggttc
    24841 tctgaagcta ttgaaaaagg aaatgcagca atatttgcag gtgcaggatt atcgatgtca
    24901 caaggatatg taagttggcc ggaactactt aatgatcctg caacagaaat cgggttggat
    24961 tctaaaaaag aaacagactt agttactctg gctcaatatt ataaaaatga aaatggtgga
    25021 tcaagaggaa ttttgaatca gatacttatg gataattttg gtgaagaatt agaaatatct
    25081 gaaaatcatc gaatactagc aagtttacct attgaaactt attggacaac taattatgat
    25141 catctaatag aaaaatcaat ccgagaagct tataaaaacc ctcaggttaa gaaaaattat
    25201 actcagttag caacaactaa tccaaatgtg gatacaattg tttataaaat gcatggtgat
    25261 atagatgatg tcagttcgac agtaataaca agagatgatt atgaaaaata tgatgattcg
    25321 tacgcgttat ttaaagaaac tttaaaagga gatttgttaa caaaaacttt tctcttttta
    25381 ggatttagtt ttacagaccc taatttagaa agaattttat cagatataag atgggttttg
    25441 agagaaaacc aaagacctca ttattgtata atgagaaaaa tattatagaa aaaattttag
    25501 gttatcgctg aaagccccgg aaatacgggc atttcgcagg atatgagaac tcaatttact
    25561 accaatttac tactttcgga ttgagccgca tatatgctga accatactgc taatacccgc
    25621 caccccaaat acggcacatc ataaattgaa tacgacaccg taaacgcggc gttcctcttt
    25681 cccgtcatgg gagaacagga cgccgctttt ttatgcccaa atagagaaag gaggcgacgc
    25741 catgctattt acttccggcc ctgaccgggc gtttgaacgg ctgatgcagg aaaagcccgg
    25801 tttcgatcac tacgacggcg gcggggcgga cgcgccggag gattgcggca cctgccgatt
    25861 ttaccgcccg gattggaaat atcaattctg ccagtttgca gagtgtccct atcagccggg
    25921 caagctgacc gactatgcag cggtcaaatt cacagtgaaa ggagcgaaca acgctatggc
    25981 agttttccgt gtggaaaaga acaaaggcta tacggtgatg tcaaatcacc acctgcggaa
    26041 taagaacctg accttaaagg ccaagggcct gctgtcgcag atgttgtcct tgccggagga
    26101 ttgggactat accctaaaag gactgtccta tatcaaccgg gaaagcatcg acgcgatcag
    26161 gacagcggta tgggagcttg aaaaagctgg atatatccgg cgtgagcagg gccgtgacgc
    26221 aaaaggcaaa atggccgata tggtctatac catctacgaa cagccggtat tggaaaaccc
    26281 ggtattggaa aatccaacat cggataaccc ggtgttggaa aatccgacat cggataatcc
    26341 ggcgtcggaa aatccaacgc aattaaataa agaagtacaa agaactgact tatcaaaaaa
    26401 agaaaaaata attacagatg gacaaagtac ccattccatt cctatcccat ccccgacccc
    26461 tgcccctatg gagggcgagg cagcggctac gccgccggaa cggaaaagga caggaaagga
    26521 tgaggctgta cggatttatc gggaaatcat tttggagaac atcgagtatg cctatctcat
    26581 tcaggacagg agcattgacc gtgaacagct tgacgaaatc gtagacctga tgctggaaac
    26641 cgtctgcacc gcccgaaaga caatccgtgt cgccggggac gactaccctg ccgagctggt
    26701 gaaatccaag tacatgaagc tgaccggcga acatatccgc tttgtccttg actgtctgcg
    26761 ggagaacaca acgaaagtcc gcaatatcaa acaatacctg cgggctatgc tgttcaacgc
    26821 ccccagcacc atttccaact actacacggc ccttgtcgcg cacgacatgg cacagcccga
    26881 ttggggaaag cctaaatccg gcctgccgga ctattcctgc ggcgagggcg aaagcctgta
    26941 acgagaaaag gaggacgacc ctatgaagca gggcgcattg atttttgacg agcgaacaga
    27001 ccgttacgac atccgctttg atctggccga ctattacggc ggcctgcatt gcggggagtg
    27061 tttcgacgtg atgatcggcg gcaaatggag gcccacccgc attgaaatgg ccgaaaactg
    27121 gtatcttgtc ggcatccggg ccgacgacct ttccggcctg cgggtgcgga tttgacctat
    27181 atctacctgc ggcgtctggt ggttccgcct tagacgccgc ttttgacttt ctgacaagga
    27241 gggatgcgat tgcaggaaga agttactcaa aagaccattg ccctgtccat gaaaacgggc
    27301 aagctcacgg ctcaagtgct gcaagcggcg ctgaaaaagt acctgcaaca ccgggccaag
    27361 ggcaaaaaca cgctgcacca cggccagcag agcctaaagc agcttaaaaa gcatggggct
    27421 gatctctcca acattgagat caccgaggcc aacattggag cgttcaagcc atgcgccaag
    27481 aaatacggcg tggattttac cttacgaaaa gataagacca cccagccgcc ccactacgtt
    27541 gtgattttca aggccaagga tgcagataac cttgatctgg cgtttcggga gtttaccgcc
    27601 aagacgcttt ccaaggagca gcggccctct atccgcaagg ttttgtccgc tgtgaagcaa
    27661 aaggcggcgg cccagaccaa gcagcgggcc aaggagaaaa tcaaggaaag ggggctggaa
    27721 ctgtgaagcc tgacgtaaag aaattgctga tcctgaacct cccgtatctg atttttgtgt
    27781 acctgtttgc gaaatgcggg gaggcgtacc ggctggccgc tggtgctgac ctgtcgggga
    27841 agctcctgca ttttgcggat ggttttgcgg cggcttttgc caaccctctg ccaagcctgc
    27901 acctgtttga cctgtgtatc ggtatcgccg gtgccgtgat cgtgcggctg gtggtgtact
    27961 gcaagagcaa gaacgtgaag aaataccgca aaggcatgga gtatggcagc gcccgctggg
    28021 ggacggccaa agacatagcg ccttacatcg accccaagtt tgaaaataat atcctgctca
    28081 cccaaacgga acggcttacc atgaccggca ggcccaaaga ccccaagaca gcgcggaata
    28141 aaaacgtgct ggtgatcggt ggcagcggtt cagggaaaac gagatttttc gtgaaaccca
    28201 acctgatgca atgctttccg accacggact atccgacttc gtttgtggtg acagatccga
    28261 aaggcacttt ggttctggaa accggcagga tgttccagca ggcgggctac cgcattaaaa
    28321 tcctgaatac cattaacttt tccaagtcca tgaagtacaa cccctttgtc tacatccatt
    28381 cggaaaagga cgtgctaaag ctggtaaata ccctgatttc caacaccaag ggcgaggggg
    28441 aaaagtcagc ggaggatttt tgggtgaagt cggaacggtt gttttattcg gccctgatcg
    28501 gctatatctg gtacgaggcc ccagcggagg aaatgaactt ctcaacgctg ctggaaatga
    28561 taaacgccag tgaagcccgc gaggacgacc cggagtttca aagccccgtc gatctcatgt
    28621 ttgagcgttt ggagcagaaa gaccccgacc attttgcggt gcgccagtac aagaaattcc
    28681 tgctgtccgc aggcaagacc cgttcttcca tcctgatttc atgcggtgcc agacttgcgc
    28741 cctttgacat ccgggaggta cgcgagctga tggaggacga cgaaatggag ctggacacca
    28801 tcggggacga aaagacggtg ctgtatctca ttatgagcga tacggacacg acctttaatt
    28861 tcattctggc tatgctccaa agccagctaa tcaacctgct ttgtgaccgg gctgatgatg
    28921 tgtacggcgg gcggctgcct gtccatgtac gcctgatttt ggatgaattt gccaacattg
    28981 gccaaatacc caatttcgat aaactcattg ccaccattcg aagccgtgaa atatcggcct
    29041 ctattatttt gcagagccag tcgcagctaa aagcgattta caaggacgcc gccgagatca
    29101 tttcggataa ctgcgatagt gtgcttttcc ttagtgggcg cggcaaaaat gccaaggaga
    29161 tttccgatgc gctggggaaa gaaacgattg acagtttcaa caccagcgag aaccggggaa
    29221 gccaaacgtc ccacggattg aactatcaaa aattaggaaa ggagttgatg tcccaagacg
    29281 aaattgccgt gatggacggc ggcaagtgta tcttgcagct acggggtgtg cgcccgtttt
    29341 tatcggagaa attcgatatt accaagcatc cccggtacaa ataccttgcc gacgccgaca
    29401 agaagaatac ctttgacgtg gacagatttt tgactatcac gcgccggaaa cggcagcaag
    29461 tggtaacgca ggatgaagta ttcgacctgt atgaaatcga cctgtcggac gaggacgccg
    29521 ccgctgaata aacagcggct tttttcattc caacaacaaa atcaaaaaat cattatgagc
    29581 cggaaaccgg cagaaagagg taaatttatg gcatttttta attccgcagt tactactttg
    29641 cagactatcg tgatcggact gggcggcgct ctgtgcgtat ggggcggcat caatctgttg
    29701 gagggatacg ggcaggacaa tccgggggca aaatcgcagg gtgtcaagca gcttgtcgct
    29761 ggcggcggcg tggccctgat cggtgtcact ctggttcccc tgctgtccgg gctgctgggc
    29821 tgatcctggc ctttgtccct gtccaaaccc ttaactaatc gcctccgcgt cctcccggcc
    29881 agaccgggag ggcgcgggaa aggaggtgct acccgtgatt agtgaaatca ttgaagaatg
    29941 gataaaaggc atcctcatgg acggtatcac gggcaacctg tccgggctgt tcgatacggt
    30001 aaatgccaaa gtcggagaaa tcgccgccga cgtgggacga accccgcagg catggaacag
    30061 cggtattttc aatatgctgc acagcctgtc ggaaaccgtg atcgtcccca tagccggggc
    30121 catactcgcc cttgtcatgt gctatgagct tatccagatg ataacggaaa agaacaatat
    30181 gcacgacttt gacagctcca tgttcttccg ctggatattc aaatccgcgt ttgcaatcct
    30241 gatcgtaacg aacacatgga acatcgtcat gggcgtattc gacgccaccc aaagcgtcgt
    30301 caatcaaagc gccggtgtga ttatcgggca gacatccatt gattttaaca ccctgatacc
    30361 agaccttgaa agccaattgg aggctatgag cgtgggcgct cttttggggc tgtggttcca
    30421 aacccttgtt gtgggcctca ccatgaatat cctttccatc tgcattttcc ttgtgaccta
    30481 tggccgcatg gtggaaatct atgttgtcac gtcgctgggg cctatccccc ttgccacaat
    30541 gggcaacgcg gaatggcgcg gcatgggcca agggtatctg aaatccctgc tggcgctggg
    30601 cttccaagcc tttttaatca tggttgtggt gggtatctac gcggtgctga tacagaatat
    30661 cggttccgct tccgacatat ccggcgcaat ctggggctgt atgggctata cggtgctgct
    30721 gtgcttctgc ctgttcaaga ccggcagtat atcaaaggcc gtattcgggg cgcattgatc
    30781 ccaaaatcca agcatacaca ggaggaactt gtttatttca acgtacaaca tcatttacgc
    30841 tgacccgccg tggcggtatg actgcaaaaa cgtacagggc gcagcggaaa atcactattc
    30901 caccatgagc attgacgagc tgtgcgccct gccggtggaa cggctggcgt ccaaagactg
    30961 cctgctgttt ttgtgggcca cattccccat gctgccggaa gctctgcggt taatcaaagc
    31021 gtggggcttt tcattcaaga ccgtggcttt tgtatggctg aaacagaacc gtaaaagcct
    31081 tacatggttt tacgggctgg gccgctggac aaggggaaac gcggaaatct gcctgctggc
    31141 aacacacggc aagccgaagc gtcgttctgc ctctgtccat cagtttatta tcagcccgat
    31201 tgaacaacac agcaaaaagc cggatgtgac ccgtgaaaaa atcgtagagc tggcgggcga
    31261 tttgccccgc gtggagctgt ttgccagaca gaaaaccccc ggctgggacg tgtgggggaa
    31321 cgaggtggaa tgtgacctta tcctgacgtg aaaggaggtg ccaaatggct tatgtaaccg
    31381 tccccaagga cttaacccat gtgaaaagca aggttgtgtt tgggcttacc aaacggcaac
    31441 tgatctgttt ttccggggcg ctggtgatcg gcgtcccctt atattttctc acccgtgact
    31501 atcttaccaa cagcgcggcg gccctgctca tggtatttgc catgctgccg ggctttttgc
    31561 ttgccatgta cgagcgtcac ggccagcccc ttgaagtggt gattatgcag atcatccagt
    31621 gctgcttcat ccagccaaag gagcgtccct atcagaccga caacacctac accgcccttg
    31681 tgcggcaata ccagatggaa caggaggtaa aggccattgt ccaaaaagga aatcccaaga
    31741 gaaaccgaaa aaacaaagct gacccgcgcc cagcgaaaag aaattgacgc ggtaatccgt
    31801 aaatataagg gcgacggcag gccccacacg gcgcagcaga gcatccccta cgaggtcatg
    31861 tacccggacg gcgtgtgccg ggtatcgccc ggcgtctttt ccaagtgcat tgagtttgcg
    31921 gacatcagct atcagcttgc ccagccggac acgcagacgg ctatctttga aaaactgtgc
    31981 gacctgtata actatgtgga cgcctccatt catatccagt tttctttcct caatcggaaa
    32041 gttgacccgg tgcagtacgc caagagcttt gagatcgcgc cgcaggggga cgacttcgac
    32101 gacatccggg ccgagtacac cggcattctg caaaagcagc ttgcaaacgg caacaacggc
    32161 atggtaaaga ccaaatacct gacattcacc atcgaggccg aaagcgtgaa agcggcgcga
    32221 gccagattga agcgcatcgg ctttgacctg ttaggctatt tcaagagcat gggggccgta
    32281 gcccatgtga tggacggctg ggaacggctg aacctgctgc atggcgtgta ccacccggac
    32341 ggcgagatat tcaattttga ctggaagtgg ctggccccgt ccggcctctc caccaaggat
    32401 tttattgccc cgtcctccct gtgcttcggc aatgcaaaga cattcggcat gggcggcaaa
    32461 tacggcgcgg taagtttcct gcaaatcctg tccccggagc tgtcggacga tatgctggcc
    32521 gacttcctca atacggaaag cggtgtttta gtcaatctcc atgtgcaggc cattgaacag
    32581 accaaggcga tcaagaccat caagcgcaaa attaccgacc ttgacgctat gaagattgcg
    32641 gagcagaaaa aggccgtccg cagcggttac gatatggaca ttcttcccag cgaccttgcc
    32701 acctacggcg aggacgccaa gaagctgctg acaaagttgc agacccgcaa tgaacggctg
    32761 ttccagttga cgttccttgt cctcaacgtg gccgacacca agcaaaagct gaataacgac
    32821 gtgttccaag ccgcaggcgt ggcccaaaag cataactgtc cccttgtccg gctggactac
    32881 cagcaggaac agggccttgc aagcagcctg ccgctgggcg tcaatcagat caagatacag
    32941 cgcagcctta cgacttccag cgtggccgtg ttcgtcccct ttgtgacgca ggagcttttt
    33001 cagggcggcg cggccatgta ttacggcatc aacgccaagt cccgcaacat gattatgctg
    33061 gacaggaagc aggcccgttg ccccaatgcc ttgaagctcg gcacccccgg cagcggcaaa
    33121 tccatgagct gtaaatccga gatcgtcagt gtgttcttaa cgacgcctga cgatattttt
    33181 atttcagacc cggaggccga atattacccc cttgtcaaac ggctccacgg acaggtaatc
    33241 cggctgtcgc ctaccagcaa ggactttgtg aacccgctgg acatcaacct gaattattcg
    33301 gaggacgata acccgctggc cctgaaatcc gactttgtgc tgtcgttctg tgagcttgtc
    33361 atgggcggca agaacgggct ggaagccatt gaaaagaccg tgatagaccg ggctgtgcgc
    33421 gtgatctacc ggccctatct ggcagacccc cgcccggaga atatgccgat cctgtccgac
    33481 ctccacaaag cgttgttaga tcagcatgtc ccggaggctg accgggtggc gcaggccctt
    33541 gacctgtatg tgtccggctc cctgaacgta tttaaccacc gtaccaacgt ggacatcggc
    33601 aaccggcttg tgtcctttga cattaaggag ctgggcaagc agctcaaaaa gctggggatg
    33661 ctcattgtcc aagaccagat atgggggcgc gtcacggcca accgaagcca aggaaaggcc
    33721 acatggtatt ttgccgacga gttccatttg cttttgaaag aggaacagac ggcggcctat
    33781 tctgcggaga tttggaagcg gtttaggaaa tggggcggca ttccaacagg tgccacccag
    33841 aacgtcaaag acctgctttc ctccccggaa attgaaaata tccttgaaaa cagcgacttc
    33901 attacgctgc tcaatcaggc gtccggggat agaaaaatcc ttgcggaacg cttgaatttg
    33961 tccaccgaac agcagaagta catcgacaat tccgaacccg gcgagggcct tttgatcttt
    34021 gaaaacgtgg tgctgccctt taccaacccc atcccgcaca acacccagct ttacaagatt
    34081 atgacgaccc gcttaaacga ggtggcggga gtatgacccg gcgacgccac aaaggaggtg
    34141 aaccaccatg aaagagctga aagccaaggc gaaagtgacg caggccatga cccgcgacgg
    34201 gctgacgctg gaaaatcagg ccaccggcga ggtggagaac atttccagcc gggaggccga
    34261 acaggatttg tccgcgccgg ggccgggcgg caccgccgat aagctgctgg aacgggccga
    34321 cgccatccat gacggccata aggccaagaa agccgcgaag caggccgggg ctgtggccgc
    34381 cgagggggac gctgccctgc accggccatc cgcccggctc caactgtcgg aggaagaacg
    34441 ggccgacccg gatttacagc cctatatccg aaaatcggac aaacgggccg ataagctgga
    34501 taaggcccgc gccgccatcc ccaagaagcg cgtcccggtc aaggaaaccg tctttgacga
    34561 ggcaagcggc aaagcaaaga ccgtcctgca ctttgacaaa caggacaagg ggcctccctc
    34621 cctgaaaccc aatccggcag gccgtcctgt ttccgaggtt tttctattcg gccacgggaa
    34681 aatccatgaa gtcgagcatg aaaacgtggg tgtggagggc ggccataagg gggaggaact
    34741 tgccgagcgg caggccggaa aagccatccg cagcaccatc cggcaccaca ggctgaaacc
    34801 ctactgggac gcggaaaagg ccgagcgcag gctattgtct gccaacgcgg agtatttcta
    34861 ccagaaatct ttgcgggata acccggagat cgcacaggcg gccagcaacc ccatttcccg
    34921 gttctggcag aaacagcaga tcaagcgcca gtacgccaag gccgcccgga cagcgggcca
    34981 gaccgcaggc gcggcggcac agggcgcggc caagacagcc ggggcctcca cgaaagcggc
    35041 caaggccgca gccaaagaaa gtgaacgggc ggcctccttt gtggcccgcc actggaaagg
    35101 ggcgctggcc gtccttgtgt tggggctttt gatgctgctt atcatcggcg gcttacagtc
    35161 ctgcaccgcc atgttcggca gcgccggaag cggcatcacg gcgtcctctt acttttccga
    35221 ggatagcgat atgttggccg ccgaggacgc ctacgcggag ctggaagccg agctgcaaga
    35281 ctatctggac aactacgagg ccacccatga ctatgacgaa taccattttg accttgacga
    35341 gatcagccat gacccctatg tgctgatttc cattctgtcg gcgctccatg acgggatatt
    35401 taccatcggg gaggtacagg gtgatttgtc catgctcttt gacaagcaat atatcctgac
    35461 agaaaacgtc gtggtagaaa cccgttaccg caccgaaacc gatacatgga cggacgcgga
    35521 gggcaacacc catacagaca cctaccaagt gccgtatgac tattacatct gctacgtcac
    35581 gctggaaaac ttcgacctgt cccatgtgcc agtctatatc atggacgagg aaacgctaag
    35641 tttctattcc ctgtatatgt ccacgctagg aaaccggcct gatctgtttt ccggcagcca
    35701 gtaccccaac gcctccaccc tgacgccgcc gacctactac gacgtacccc cggaggcttt
    35761 ggaggatgcc acctttgccg ccatgctcaa agaggcggaa aaatatatcg gttatcccta
    35821 tgtgtggggc ggcagctctc ccagcaccag ctttgattgc agcggcttca tttcgtgggt
    35881 aatcaatcat agtggatgga acgtgggacg gctgggggcg cagggcctat ataacatctg
    35941 cacccctgtt tccagttcgc aggccaagcc cggcgacctt gtatttttca agggaaccta
    36001 cgacaccccc ggcgtgtccc acgtcggcct gtatgtgggc aacaatgtga tgctgcattg
    36061 cgggaacccg atttcctaca cgaaccttac ttcaaactac tggcaatccc atttttacag
    36121 ttacgggcgt ttaccttaac gccagaaagg agctttcaat tatggcaacc aaacttgacc
    36181 gcatcgaaaa ggacatccag aaaaccaagt ctaagatcgc ggagtttcag aaacagcttc
    36241 gggagctgga aacgcagaaa accgaacagg agaatttgca gatcatccag cttgtgcgcg
    36301 gcatgaacat gaagccggag gaatttgccg cgttcttacg cagcggcgca atgcaggccg
    36361 cccctgccgc aaccccttac catgaacagg aggacaatgc ccatgaagaa taagaccaga
    36421 cggatttccc gtattctccc cgcacttctg gccgtgatgc tctgcgtcac ggcctttccc
    36481 gttaccgccc ttgcaggcgg caatgacccg gcaccggccc cgctgccgga ggctacaacc
    36541 gaacccgcta cgggcggttt ggagcctgaa acagacgaaa ccggcaccgc cacggacggg
    36601 acagaaaccg aggccgaaaa agataccggc cctctgaccg gcaaggacat caccgacctg
    36661 ttttccgcgc tgttcggttc caaggtgagc attgccgcca cggacgacgg catccagatc
    36721 accaccggca ccgacaaaaa agaacctgaa cagaccggca ccgtcaccac caacggcggc
    36781 aggctgaatg tccgcaccgg cgcggggctt gacaagaccg cgttcaccca gcttcccaat
    36841 gggacgacgg ttgaggtagt cggcacagac ggcgactgga ttaaaattct cttgccggag
    36901 cgcatcggct atgtccattc cgactatatg accgtcagcg aaaaggaagt agcggctacc
    36961 ggcgagggcg gtttttccct ctccattgac ccggaggaaa ttgcttctct gctggaactg
    37021 ttcaatggcg gcggggccag cgccgccctg tccccggacg ggaacctgtc actgattgac
    37081 gacatcggca gcgccgcgaa aaatggcaag cagtttatca ccgtggaaac caaaaacggc
    37141 aatgtgtttt atctgattat tgaccgggac gacgagggaa aagaaactgt ccatttctta
    37201 aatcaggtgg atgaagccga tctgctgacg ctgatggagg acggggaaac cgtcaccccc
    37261 gctgctgtct gctcctgtac cacgaaatgc aaggccgggg ctgtgaacac gaattgcccg
    37321 gtatgtaaaa ccaacctgac cgagtgcagc ggcccggagc cgcaggaacc ccagccggag
    37381 gaacccgaag ccccggagga agaacccaag ggcggcgcgg gcggcctgat cgtgttcctg
    37441 ctggtggcgc tggcgggcgg cggtgcggcc ctttactatt tcaagttcaa aaagcccaag
    37501 gctgaaacca ccggcagcga cgatttgggc gagtatgatt ttggggagga cgaggatttg
    37561 gaggacgagg aacccgaaac cgagtttgac ccggaagctg attttgtgag tgaacaggag
    37621 gacgaggaat gacatataaa cttgtaatcg cggaaaaacc cagcgtggcc cgcagcattg
    37681 cgggcgtgat cggggccacg gagaaacacg acggctattt ggaggggaac ggctatctgg
    37741 tgagctggtg catcggacat ctggtgtcct ttgccgacgc cggacgctat gacgagcgtt
    37801 tcaaaaaatg gcgctatgag gatttgccca ttctcccgga aacatggcag tacatcgtcc
    37861 ccgacgacaa gaaacagcag tttgacgttc tctgttcgct gatgcagcgc cccgacgtga
    37921 ccggccttgt gtgcgccacc gacgccgggc gcgaggggga attgattttc cgtttcgtct
    37981 accagatggc gggatgccaa aagccctttg agcgcctttg gatttcctct atggaggaca
    38041 gtgctattaa ggacggcttt gcccacctga aaccgggcgg cgactatgat ccgctgtatc
    38101 aatcggccct gtgccgggcc aaggccgact ggctgatcgg cattaacgcc acccggcttt
    38161 tttccgtcct gtaccacaag aacctgactg tgggccgcgt ccagacgccg accttaaaaa
    38221 tgctggtgga cagggacgcc aagattaccg atttcaagaa agagaaatat cacatcgtcc
    38281 atatcacggc gggcggcgcg gacgcggtaa gcagccgctt ttccgacgct gccgaggcaa
    38341 acaccgtcaa ggcggcttgt gcaaaagcgc aggccgtttg cgtttccgtg acgcgggaga
    38401 aaaagacgga acagccgccc aagctctatg accttaccac cttgcagcgg gaggctaacc
    38461 ggcttttcgg ctataccgcc aagcagaccc ttgactatgc ccaggcactg tatctaaaga
    38521 agctgctgac ctatccgaga acggatagtc aatatcttac cgacgatatg ctgcccgccg
    38581 tggaaagcct tgtgtccggc ctgtgggagc tggtgccgtt tgccaagggc ctgaaccttt
    38641 ccccgcagtt tgaccgcatc ctgaacagca aaaaggtgtc cgaccaccac gccattatcc
    38701 ccaccgctga atttgtgaag cagggctttg gcactctggc cgagagtgaa aaaaagctgc
    38761 tgtccctgat ctgctgcaag ctcttatgtg ccgtggccgg gccgcatatc tatgaggccg
    38821 tcaccgccac atttacctgt gccgggcagg agtttaccgc caagggcaag accgtccttt
    38881 cccccggctg gaaagagatt gaacgccggt tccgttcttc cttaaagacc gacgccgacg
    38941 aggacgccga ggcggtgcgg gagctgccgg agctttgcga gggccagacc tttgcagacg
    39001 tggccgcctc tgtcaccgag catttcacca cgccgcccaa gccctacacc gaagatacgc
    39061 ttttgtccgc tatggaacgg gccggggccg aggatatgcc ggaggatgcc gagcgcaagg
    39121 gattaggcac accggccacc cgcgccgcca ttctggaaaa gctggtgcag atgggctttg
    39181 tccagagaaa aggcaagcag ctcattccca ccaaggacgg catcaatctg gctgtggtgc
    39241 tgccggaggc gcttacttct ccccagctta ctgccgaatg ggaaaaccgg ctgaccgaga
    39301 tcgccaaggg gcaggccgat cctgacgaat ttatggacgg catcgagacg caggcgcggg
    39361 agctggtgca gacctattcc tgtatcagtg aggaaaagca gaaattgttt caggccgagc
    39421 gtgttcctat cgggacgtgt ccccgctgtg gtgaggctgt ctatgagggc aagaaaaact
    39481 actactgcgg aaaccgggcc tgccagttcg tcatgtggaa gaatgaccga ttttttgagg
    39541 aacgcaagaa agcgttcacc ccgaagatcg ccgctgcgct gctcaaaacc ggcaaagtca
    39601 aggtaaaggg cctctattcc gtaaagaccg gcaaaaccta cgacgggacg gtgcttttgg
    39661 ctgataccgg gggcaagtac gtcaactacc gcattgaaca caggagctaa aggacagtaa
    39721 cacagcaagc atagggagtg tggctacact cccggtaaaa tcccccggcc gcgtccctgc
    39781 cgggccgggg atttttgctt gttggggaag tccccaaccc cctatttttt gaaacagaac
    39841 agagaaagga gtgaccgcgt gaacgaagta ttttctatcc aagtccataa ccggcagttt
    39901 ttggaacagg gcaaacaagg cgtctggctg gatttgccca ccacaaccga aacgctgcaa
    39961 gccgccctgc gggatgttgg cattactgcc gacaatccgc aggatttttt tatcaacggg
    40021 tattcctgcc cggaggacag acaccttgcc attccctatg acatggtgct ggccgccgac
    40081 gtggacgagc tgaatttcct tgccgcccgg ctgggccagc ttgacgccgc cgagatcgca
    40141 gagctgaacg ccgccctgca aaatccccgt ggcggctttg agaatatcgg ccagatcatc
    40201 gactacaccg aaaatgtgga ttactatgtg catcttcccg acgttagggg ggatggacag
    40261 ttgggcgact actacctgaa ccgttccggc atggtggata tgccggagga atggaaagcc
    40321 gggattgata cggcgcagtt tggacgccac attgcccagc aggaacaagg cgtttttacc
    40381 cagtacggct atctggtgag aagcggcgac gaatggcagc gcgtccatga gggccagccg
    40441 gtgccggagg aataccgggt gatgcactac ccccagccgg aagctgaccg ggaggaaccg
    40501 caggcccagc agccggaggc atccgaaagc cccgacaaag cgccgccccc ggttatcccg
    40561 atcatgctgg acggcatgaa cagcaaagaa cgcatgaaag tgattactga ccgtctggaa
    40621 accggcgtac aggagctttt tgaaagtagc cgctatatgg cttacttatc cgctatgtcc
    40681 aagatgcacg attacagtct gaacaatacg ctgctgatcg ccatgcaggg cggttcgatt
    40741 gtggcgggct ataacaggtg gcaggatgaa tttgaccgcc atgtgaagcg gggcgagaaa
    40801 ggcattaaga tactggcccc ggccccgttc aaggttaagc agcagatgga gaaaaccgac
    40861 ccggccaccc agcagcccgt gatcggcgcg gacggcaagc ccgtgaccga agaacaggaa
    40921 gtgacggttc cggcctttaa ggtggtgaca gtctttgaca tcagccagac cgagggcaag
    40981 gattttccca tgccctatat cagtgaactt accggcgacg tgcagcagta tcaggatttc
    41041 tttgccgcgc tggaacggac atcaccctat cccataagca ttgagccgat ttcagacggg
    41101 gccaagggcc gctgcttcta cgcaaaacag cgcattgcca tcaacgaggg catgagcgaa
    41161 ttgcagaccc taaagaccgc catccatgag attgcccatg ccaagctcca cgccattgac
    41221 cgggaggccc ccgccgagga acaggcggcc cggccagacc gcagcacccg cgaggtacag
    41281 gccgaaagcg tggcctatac cgtctgccag tattacgggc tggacacgtc ggactattct
    41341 tttggctatg tggccgattg gagcagcggc agggagttat ccgagctgaa agcctccctt
    41401 gaaaccatcc gggccacggc cagcgaattg ataaccgaga tcgacggcca ttttgcccag
    41461 cttcagcagg agcgcgaggc ccggcaggaa caggaggccg tcacggttga cgttaccccg
    41521 gaggcagcgg ccccggcgca ggccgaggaa gaacaggccc cggcccttga tccggcagcc
    41581 gagccgcttg tgactgtgct atggagcgaa agcccccagc taaaggacgg ccagcaaatg
    41641 ccgttgcatg aggccgacgc gctgtttgcc cgtctggatg cagcatatcc ggcagaggca
    41701 ggctatgaca aaaccgcgtt ccgcattgac tgtatctttc agggcgagcc ggacagctac
    41761 gaggggcggc aggattttgg agatagggac ggttccctga tcgaacatat ccagagctac
    41821 caccaatttt acgcgcagga cgaaaactgg aaaaagcatg taatcgctac aaccggcata
    41881 gagggctggg agcaggacaa ggcccatcgg gatatgctgt taggcgagtt tgtcccctat
    41941 atgaagctgc attgcagcct tgcccaaatg gaacagacgg cgcagggccg cttacagtcc
    42001 ggggaaaccc tgacgccgga ggaaacggcc tatttggggg cgcttctctc ttatgtgcag
    42061 gagtgccgcc ccatgctcaa tcagggccag tatcagttgc cggagccgcc gaaactggct
    42121 gacttcgacc agagcttgca ggactacaaa gcacaggtac aggccgagat cgcgcaggag
    42181 gccgccgcag ccggtatgac cgtggaggaa tacgcggcgg ctggctatga agcaccccaa
    42241 caggacacct tttccatcta ccagcttcga ggcggggagg aaacccgcga ctatcggttt
    42301 gagccatacg agcgtttaca ggccgcaggg ctggccgtgg acagtgccaa ctatgacctt
    42361 gtttatacgg caccgctgga taaaagcacg tctttggagg atatttaccg cacattcaac
    42421 atcgaccacc cggcagattt taggggacac agcctttccg tttccgacgt ggtggtgctg
    42481 caccagaatg gacaggacac cgcccactat gtagacagct tcggctatat ggaagtgccg
    42541 gagtttttgc gggaaaatcc cctgcgtacc gccgaactgt ctacggagca gaacgaaaac
    42601 atgattgacg gcattatcaa caatacgccg cccgaaccgc agccggagga acccgaacag
    42661 ctatcttttg aggatgtggc agttgcttcc ggccagacac aggaggaacc cccggaggct
    42721 gcgcccccgc aaacggctgt ccgctattat cccatcaatg agggagccgc ccgccgcgcc
    42781 aaggaagcca tgagcttttc tgactaccgg cccggcagcg ccacggcgga ataccggcac
    42841 tatgtcgatc aggccgtgga aattgcggag cgccagaaaa agcgtgtgga gccagagtat
    42901 cacgaaaaga tagacagcct ccttgacacc tacgcccgca agctggccga gaacatgaat
    42961 aagggctatg agattaccgc ccgcgtcccc tctgtcctga ttgccgggcc gtccaatttc
    43021 ccggtacggg cgaaagaaaa gcagaacgcc gccagtgacc gcaacatgga ggaatttcag
    43081 tacattcagg gcttgcttga caaaatccgc agcaccggca tgggtggtat cagcgccgac
    43141 gatccgcagg ccgtttccaa gctggaaaag aaactggaaa agctggaagc gtcgcaggag
    43201 ttgatgaagg ccgtcaatgc ctactaccgc aagcatggga cgctggacgg atgcccccat
    43261 ctgatcgaaa gaggcattga aaacctgaaa gcggatatgg cgtcgggctg gcactatgag
    43321 aaaaaaccgt tccagtcctg gcagctctcc aacaataacg ccgagatacg ccggttaaag
    43381 ggccgcattg aggaattgac ccgccaaaag gaggcggcct atgtgggatg ggagtttgac
    43441 ggcggcactg tggagatcaa ccgggtggcc aaccgcctgc aaatcttctt tgaggggaag
    43501 ccggatgctg ctgtacgcga cgagctgaaa agcaacggtt tccgctggtc gcccaaggcc
    43561 ggggcatggc agcggcagtt aaacgacacc acaatccgcg tcgctgaccg tatcaagtgc
    43621 atccagcccc tttccggcga aaagcccagc gccttacagc ttgcggcccg cagggagaaa
    43681 gaaaagccct ctatccgggc gcagctcaac gcggccaaga ccgaacaggc caagcagcca
    43741 ccggcgcggg aaaaatcaaa gggattggag gtagagtaaa tgcagaagtt tgaaaatgtg
    43801 gacgttctgg ccgcgctgga acagatcatg cggcagaaca ccgcgtttta tcaaagtgac
    43861 tttggcattg acaaagacat tatcaggcgg gatgccgcca gcgatcaggc cgcagacaag
    43921 accctgctct ggatgtcccg gccctccggg acgtactgtt tccgggaacg tgatgttttc
    43981 ctgaaagaca cccgacagta caacacatgg aaattttacg gggagcagac ccgtgacaag
    44041 atactggcct acgcggtgga gctgaccggc acacagggcg gcactatccg gggcaatctc
    44101 tatgagctgg actatccgca gcatttccgg cacgtcattg aaaccgccca gccggtgagt
    44161 gtgaaccggc tcttttatga gcatggcacc cgtgacatcc cggagagcca gcgttttgac
    44221 ggaagccctg accgggtatt gggaaacttt ttgtactatg aggcccagcc ccatgacccg
    44281 gctgtgctgc aggaggcgct acggacggag cagcacagcc gggggcgggc cgtccccggc
    44341 gatttcaaag tccacgtcac cgctttgcat gacagccgga ttgaaaccga ggcccggcgt
    44401 attgtggaca gcctgaaaga tctgtccgcg cctaacagcc ccaataaaac gcatttcatg
    44461 gtggagatct ccccgcactt tgcggcgatt gcttccacca aggataatga ccggctcttt
    44521 gccatgctgc cctataaatc cctgtgtttt accgggatga aaggcaggca tggcgtgtat
    44581 gccgtgatta accgggacga gcgccgggat gtgagtattc gcaggccccg gccctctatc
    44641 cgcaagcagc tatccgacag caaacaggcc gccagcccaa aaaaggcggc ggccagaacc
    44701 aaaaagaatg aattggaggt ataaggaatg atgcacttta ccgtggagga agaaaacctg
    44761 atctgtatgt accacaatgc agaccggcgc agaacgataa acaggctcac cgccgtcatg
    44821 ccggttatgg atggggatat gcgggcgcag gcccagcaga ccctttccaa gctggaacgc
    44881 atgacggatg ccgactatga cggccagaag ttccacttca cagacgaata acgaacagca
    44941 gacgccgggc gcggcggcca tgtggtcgct tgcgtccggc gtcttttctt tttgcctatt
    45001 cctacgcatt tagaacccgg tattgtcggg gataggatga agtattcgct tgcccctgca
    45061 aacatgcggg agaagccttg cagcgggcgg ttttcaatcc cttaaatgcg taggttgcag
    45121 ctataattag acccctgaaa cagatatagc cgcgcagcgg gtgtatctgt ttcaggggtg
    45181 tgcaggtgat agccgcaaag cggcgtaaga gggtgcagcc ctcttgccgt ctatctctgt
    45241 tccagcggga agctcggcaa tgcttccagc agtttcaggg aaatgcgctg ccgtatatct
    45301 tcgtcaatga ctttcttctt tttgccgttt gcctgtctga tctccaccgt ggaaagctcg
    45361 ttgatgtagt cagcataatg ggccaacact ttttccgtgg cccacctctc accggcaacg
    45421 gcggctttga tcgtttcata gtccagcagc ttttcttgtc cgtccatcct gatacccccg
    45481 tcagactgat tttttctatg ataccatatt cggaagcatg gaaccagaga aaacggcgct
    45541 tttctgctgg taaattctac gcatttagaa cagcacttcg gcggggatag gataaaacac
    45601 ccacataccc cgctgaccat gccggaaaag ccttgaaata taagggcttt tacagctcta
    45661 agtgcgtagg tttccagtat cttttccgct ggcgctcggc ctctttgcgg cggcgcatcc
    45721 gtttggagca ggccgcgcag tatttggccc ggttagactt cggcgtgaac gtggccccgc
    45781 actcacagca gcgtttcact tcgtcccggt ctttggtgat ctcggcatac agggccgcat
    45841 ccagcgggag gacagcggcc ttaaaccagc ggcacagaag cgaataggaa atgctctgta
    45901 cgcaaataca ttcttcgccg tcgtccagca acaggcagtt gccgtcacaa tagttgcagc
    45961 actcatgcgt cagcttccgg gccttgcgat attgagggta attcattcgt ggaatactca
    46021 tagctcttgc gtccattcct ttcccggcgt catacgcaaa atgccgtcca cattctgctt
    46081 gatgatgccg tattcctgca tctgcttttt cgcttcgccg tacttctcat acagccgttc
    46141 cttttcctcg gccaatcgct tgtactcggc tttcagggcg tacagatcgg ggagcttttt
    46201 cacaccctgt ccctttaggg cctttgccgc agcttcatac aaaatcaacg tgctttcatg
    46261 ctcacgccgg aattttgcct tatccgctgc attgcggtat tccatagcca cgggccagag
    46321 ctgcttgtag gtagaaatat tctttatcag caccgccata tccgcaagcc ggtgttccat
    46381 agctttgagg gtagcggcgg cctcgtcgct ggcggcggtg atctcggcca ctttgctttc
    46441 caaatccggg taggtgtcta tcccatgttc agtcaggaaa ttcaaagtct ttgcggcctg
    46501 cttcaaattg tggagcttcg cccacctctc atagcctgcc gactgctggg ccttaatgtt
    46561 gttttccaga tcaatccgca aagaaatccc cttgcgctct ttgggcgctc tggtgataac
    46621 gcggcctttg atacgctctt tgatggcttc ctcggtgtag gcttccccca atgtcttgca
    46681 gcgggtaaag cgttcctgtc cgggagcgcg gaaagaaata aactttcccg gcttgatctc
    46741 atagccctgc gcctgcatga gccgcaggaa atcatcaaaa tcttttgcct gcgggatagc
    46801 ggcgtcaatg gctgctttga gcttcgcttt ccagctcgtc cctttccgct gggcgtccca
    46861 ctcggcatag ctcttgccct tactctggcc gggcttcacc acggacaatc cgttttcctt
    46921 gcacagcctg tcgctggtgc gccggatata ggcatagctc tgtttgttgg aaatgtactt
    46981 gcgctgattt accatatcca cggcacagaa aataatgtga ttgtggacgt ggcccttatc
    47041 aatatgggta gtcagcacat agggatattt cccttgcagc acttcctcgg caagctgctt
    47101 cccgatctca tgggcctgtt ccggcgtggc ttcgccgggg gcaaaggatt gtatcaggtg
    47161 gtggcccaga ttattgccct tttggtatgc ctgcgccagc agcatttcaa attcaatgtc
    47221 cgctgtttca taagagcagg caaaggaggc caccagcagc ttgccgtcag ttttgtccgg
    47281 gttctcgatg tagtcaaggg cttttttcag cgttgacttg ataggatgta tctttgtaac
    47341 tgccatatct gcgccagcgc cccctttata tcctcgatgt cctgtgcgta caccggcccc
    47401 ataccgttca gcctctttgc aatctgattg acgttgacgc cgatcttctg tatctcggcg
    47461 gtctgcgccc ggatgtcgct ggtgtccatg ttgacgatat agccgtcaat gagcatcttc
    47521 cgggcgtagg ctgaaaagtt cttcgtatgc agcagcgcca ttttctgttc aatcatagcc
    47581 cgttcctccg gcgtcacagg gacgcgcagc acgatttttc gctttctgtt tgccatgctt
    47641 caccgtcctt tccattcagg ggtttgggga tttccccaac aagcaaaacg gacgcggccc
    47701 ggcagggacg gggcaggccg tttttccggg agtggctaca ctccctatgc ttgctacgtt
    47761 actgtttctc cactaacaag acgtttccaa agccccaaac tacccgcaaa atagccctct
    47821 ggaaagaaaa aagtgcaaaa aaatagaagc tacgctgtgc agctcccgct aaaagtaaaa
    47881 cagaggatgc cagttacaca tcctccgttt cattcttctt cggcttttca attagtatgg
    47941 cgctgtcaat cgcgccctcg atgatcggca ggtattcttc ggggcagagt ttcagcttgt
    48001 ggctgaccct ttgccgcagt tcgctttcct cccgcatgat ctccgggtta aagtagcgtt
    48061 ccacaggaag cccgcaggct ttaataagct gtatgacaac cggcagactg ggaatggccc
    48121 gtcgttttcg atgttggcaa gatagcgcca gtcaatattt attttctctg ccattgcttt
    48181 gcgcgaaacg cgctttgctt gccgggccgc tttcacatcc gcgccgaaag tttcaaagcc
    48241 gggacaatct tcaatattcg ccatatcgca cctcccactt acattgtata tttcatactc
    48301 tgcccgtgga atgttggtat atgcaccttt cttaaagttt atatttcaca ttttgtttct
    48361 tttattcttc gagcaaaaga attataatgt accctgtgga ggtgtactat ggactacatg
    48421 acgttgaaag aggcaggcga gaaatggggt gtgcccccac gatggataaa ctactattgt
    48481 gctgcgggac gcatccccgg cgctgtaaag atggcgacga tctggctcat tcccaaaact
    48541 gcggaaaagc cgattgacag gcgtacaaag aaaggaaagg cggttactga atagtataat
    48601 gaaaccggcg cattttggat atggttttga agagagagaa agcatggtat tttgtcacgg
    48661 aaaaactata agatgataga tggcaggctg ctccagaccg ataaaaagta ttctcatctg
    48721 aaacagaaag aaaagattta cacatggatg tatgaagaag cgaaacttta tcatgacaga
    48781 aatggaaaat gcccgaaaca aagacgagga agctgctgct gtatcagccg tatatggtag
    48841 aattgaaaag gccggcatct ggattcttta tggtgaagtc tccagacatt ggataactgc
    48901 tggtctaatg cgcctttgag tatggggtga ttgattttaa taccggggag gtacggcttt
    48961 ttgaggaaaa tattctggga tttacatttg gcgattatta catttcctgt aactgcaagg
    49021 acacggatga tgaagatatg aggaatatag acttgctgat tttctactgt cttgaacggt
    49081 attaggactg aatctgctgc gcggagtgcg gcaagagcga ccggagggag tacacacagg
    49141 ccctgcctgt gtgaaaagcc ataccgcaag gtgcattcgc aaaaaagagg ccgcgcattt
    49201 tgctgaggct tggggtgtcc ccaacaagca aaacgagaac gccgcaggcg ggcaagtggt
    49261 ggatcagaat atccgtaaag gatattctga tccattgctt gccagtatta tcgggatgtt
    49321 ttgacaaccg ctgtaatcga aacatcccaa acattaagaa ggtgttaatt attattatct
    49381 tgatttaatt cgtttaaacc aataatatag atacatacac catgaaaaga agcgagtatc
    49441 ccaatgacgg atgattaaaa tccgaaaaga aatcgctttg aaactacagg gaaactacag
    49501 actgttatgt tatcttctta aaatggaggg atttttatgt cgatacgaat tctacttgtc
    49561 gaggatgatg atcatatctg taatacagta agggcgtttc tggctgaggc aggatatcag
    49621 gtggatgcct gcacagatgg aaatgaggca tacaccaagt tttacgaaaa cacttatcaa
    49681 ctggttattc ttgatattat gctgcccggt atgaacgggc atgaactttt gcgtgaattt
    49741 cgtgcgaaaa atgatactcc cattctgatg atgacagccc tgtcggatga cgaaaaccaa
    49801 atccgggcgt ttgatgcaga ggcagacgac tatgtaacaa agccattcaa gatgcagatt
    49861 ttactaaagc gggtggaagc cctgctgcgg cgcagcggtg cgctggcaaa ggaaatccgt
    49921 gtcggcaggc tgacacttct gccggaggat ttcacggtac tttgtgacgg tacagagctg
    49981 cccctgacac gaaaagaatt tgaaatcctt ttgctgctgg tgcagaacaa aggcagaacc
    50041 ttaacgcatg aaatcatttt gtcacgtata tggggatatg actttgaggg tgatggcagc
    50101 acagtccaca ctcatatcaa aaatttgcgg gccaagctgc cggaaaatat catcaaaacc
    50161 attcgcggtg taggataccg attggaggaa tcataataat ggaaagaaaa gggattttca
    50221 ttaaggtttt ttcctatacg atcattgtcc tgctactgct tgtcggtgta acagcaacac
    50281 tgtttgcaca gcaatttgtg tcttatttca gagtgatgga attacagcaa acagtaaaat
    50341 cctatcagcc attggtggaa ctgattcaga atagcgatag gcttgatatt caagaggtgg
    50401 cagggctgtt tcactacaat aaccaatcct ttgagtttta tattgaagat aaagagggaa
    50461 gcgtactcta tgccacacca aatgccaata catcaaatag ttttagaccc aactttcttt
    50521 atgtggtaca tagagatgat aatatttcga ttgttgctca aagcaaggca ggtgtgggat
    50581 tgctttatca agggctgaca attcggggaa ttgttatgat tgcgataatg gttgtattca
    50641 gccttttatg cgcgtatatc tttgcgcggc aaatgacaac gccgatcaaa gccttagcgg
    50701 acagtgcgaa taaaatggca aacctgaaag atgtaccgcc gccgctggag cgaaaggatg
    50761 agcttggcgc actggctcat gatatgcatt ccatgtatgt caggctgaaa gaaaccatcg
    50821 caaggctgga ggatgaaatc gcaagggaac atgagttgga ggaaacacag cgatatttct
    50881 ttgcggcagc ttctcatgag ctaaaaacgc ccatcgcggc tacaagcgtt ctgttggagg
    50941 gaatgcttga aaatatcggt gactacaaag atcattctaa gtatctgcgc gaatgcatca
    51001 aaatgatgga taggcagggc aaaatcattt ccgaaatact ggagcttgtc agcctgaatg
    51061 atgggagaat cgtacccata gctgaaccgt tggacatagg gcgcacggtt gccgagttgc
    51121 tgcccgattt tcaaaccttg gcagaggcaa acaaccagcg gttcgtcaca gatattccag
    51181 ccgggcaaat tgtcctgtcc gatccgaggc tgctccaaaa ggcactatcc aatgtcatat
    51241 tgaatgcagt tcagaacacg ccgcagggag gcgaggtacg gatatggagt gagcctggtg
    51301 ctgaaaaatg ccgccttttt gttttgaaca tgggcgttca cattgatgat actgcgcttc
    51361 caaggctgtt caccccattc tatcgcattg atcaggcgcg aagcagaaaa agtgggcgaa
    51421 gcagaaaaag tgggcgaagc ggtttaggac ttgccatcgt acaaaaaacg ctggatgcca
    51481 tgagcctcca gtatgcgctg gaaaacacct cggatggcgt tttgttctgg ctggatttac
    51541 cgctcacatc aacattgtaa atatttaaaa cttaaatgat tttgggctct ttgtcaaata
    51601 ggactgatag agtgagataa gaacatctaa ggtagaattc cttttctact ttagatgttt
    51661 tttgattcat aggtaatcag attatttagt aagaaaattc tggtcttcat tttccgaaag
    51721 cttcgaaagc cataagcgac tcgtttcaat gctttgatgt gggtatgcag attttctatt
    51781 ttggcatttg aataaggcaa ttcgagggct aaacgaatgc cggattcata gtttataaaa
    51841 gcttttttta tagaatgcag ttcttttgga acggaattgg gcatcgttct tacaagttta
    51901 aagaaagtgt ccgcctcttt gtcgtgaaac gcttgaagaa tttcttgata gtaaaaatag
    51961 gcgattcgta attcgtcaga gatcgaaagc agacgatcca ccacttcact ttcggtgaga
    52021 tacgcccaac caaaattgcg ccatttggtc catttgtcat aaacaagctt gtcctgtcgt
    52081 ttgatcagaa gccgagacaa cgcctttaat tgtcgataat atttggcttg tgttggatcg
    52141 tttcgatcaa agcttttcat gattcgtacg cgaatatcct caaatttacg aatgagatgt
    52201 ttgacaatgt gaaaacgatc cacaacgagt tttgcctttg gaaaacattc ctttatcaga
    52261 gaggcataag aaacattcat gtccgtcacg atgaccttta ccttcaatcg tgcgcgtcgt
    52321 tcaaattgca taaaataaga aacgagagcc tttttcttcc gtgaaggaag aatatccagt
    52381 aattttccag tatcgccatc catacaagaa aaggagtagg gaccgacttg atttttcagt
    52441 gaacgaaatt catccattaa taaacaacgc ggtaaccaat ttctttttac ttttgtcatg
    52501 gggatcagag aagcgagtgt ccgagaaacg gtcgaccaag aaacgccgca atgcttagca
    52561 attgtcgtca ttgcttgttt ttcagttagc tcttctaaaa ttttatagcg cacttgttta
    52621 gcgataaagc agtttctatc gacaatgtag gtattcgccg taaaataggt gtggcagcca
    52681 cgacagtggt agcgttgttt cgctaaagca agataagtca gttggttcgc acatttatta
    52741 agttgaatga gcgatttttt ctttccgttc ttaacgatca tgccttcatg attcatctgt
    52801 ttacacaaag gacagcgacg aatttcttta cttaacgtcg cagaaacaac aagactcttg
    52861 atattcttaa tgctcttcga ttccacattt gtgatgaaaa tgtttttatc tttgattcca
    52921 agtagtagtt tgatatgatg aagatcaggc atacagaacg ctcctttgat tgtgttgtgg
    52981 tgattcaatt ctaacggagc ttctgtatgt tttgtttttt catgctcaaa agggaccgat
    53041 agaatgattt tctatcagtc ctagaaatta tagagccgat tttgaccgac aggtataacc
    53101 ctgccggtct ttttttgctg ctacaggaaa actacagact ggctacaggg aaagtacaga
    53161 tgcgcttgcc ataataacaa tcgtaccagc cacaaatcgt agttttattg caaaggaggc
    53221 attcgatcaa atggaaaaaa gcaactatca ttccaatgcg gatcatcaca aacggcatat
    53281 gaaacaatcc gtggaaaagc gggcttttct atgtgcgttc attatttcgt tcacagtctg
    53341 cacgctgttt ttggggtgga gactggcttc cgtattggag gcaacacaga taccgcccat
    53401 ccctgcaact catacaggca gcagcactga cgtagtggag aatttggagg aaaacgctct
    53461 tgccaccgcc aaagaacagg gagatgaaca ggaatggagc ctgattttag tgaacaggca
    53521 gaaccccatc cccgcacagt acgatgtgga gcttgagcaa ctatcaaatg gtgagcggat
    53581 agatattcgg atttctccct atcttcaaga tttgtttgat gccgcaagaa ctgatggagt
    53641 ttacccgatt gtcgcatccg gataccgaac aacagaaaaa cagcaagaaa ttatggatga
    53701 aaaaattgcc gaatataagg cgaaaggcta cacctctgca caggctaaag cggaagcaga
    53761 aacttgggtg gccgtgccgg gaacgagcga gcatcagctt ggtcttgctg tggatatcaa
    53821 tgcggacgga attcattcaa caggcaacga ggtttataga tggctggatg aaaacagcta
    53881 tcgttttggt tttattcgtc gctacccacc agacaagaca gagataaccg gtgtgagcaa
    53941 cgagccgtgg cattaccgat atgttggcat cgaagctgcc acagagatgt acaaccaagg
    54001 ggtttgcctt gaggaatatt taaaaccaga aaaatgagaa aaggatgtta tgttatggac
    54061 agaaaaagat tgacacagcg tttcccgttc ctgcttccaa tgagacgggc gcagagaaaa
    54121 atgtgctttt atgcgggaat gagatttgac ggctgtcgct atgcgcagac gataggggaa
    54181 aaatcgcttt cccatttgct ctttgaaacg gattgcgcat tatacaacca caataccgga
    54241 tttgacatga tataccagga aaacaaggtg ttcaacttaa agctggcagc aaagacctta
    54301 aacggcttat tgataagacc gggggaaacc ttttctttct ggttgttcct gcatacgcca
    54361 ttgacaatta tccagcgcag aggtcacgaa gtaaaggagt ttccagagcc aaacagtgac
    54421 gagattaaag gggtggatgc aaccatttca gagggctgga ttgatttaaa agtgcgaaac
    54481 gataccgact gcacctacca aatatgggtg accttagatg atgagaaaat catcggtcag
    54541 gtgttcgccg acaaagagcc ccaagcatta tacaaaatta caaatggcag tatccagtat
    54601 gtccgtgaaa gtggcgggat ttatgaatat gcccaggtta aacggatgca agttgcctta
    54661 ggtaccgggg aaataataga ttgcaagctg ctttatacaa acaaatgcaa aatctgttat
    54721 cccctcccgg aaagtgtgga tattcaggag gagaaccaat gagaaaaagt gtgggcatta
    54781 ctgtttttgg atgcgaacag gatgaggcaa atgctttccg cgctttatcg ccggattttc
    54841 atattatccc tacgctgatt agcgatgcga tatcggcaga caacgcaaaa ttggccgctg
    54901 gcaatcaatg cgttagcgta agccataagt ccgaggtttc cgaggcgaca attcttgcgc
    54961 tgagaaaggt cggggtaaaa tacatttcta cccgcagcat cggctgcgat cacattgata
    55021 cgactgccgc cgagagaatg ggaatctcgg ttggcacggt tgcgtattcg ccggacagcg
    55081 ttgcggatta tgctttgatg ctgatgctga tggccatacg gggtgcaaaa cccaccatgc
    55141 acgccgtggc gcaacaagat ttcagattgg atcgtatccg ggggaaagaa ctgggggata
    55201 tgactgtggg agttattgga accggccata tcgggcaagc ggtcgtcaaa aggctgcggg
    55261 gatttggatg ccatgtgctg gcctatgata acagccgaaa aatggatgca gattatgtcc
    55321 agcttgatga gcttctaaaa aacagcgata ttgttacgct ccatgtgccg ctttgtgcgg
    55381 atacccgcca tctgatcggt cagaagcaaa ttggagagat gaagcaaggc gcatttttaa
    55441 tcaacaccgg gcgcggagcg cttgtcgata ccggggcgct ggtggaggcg ttggaaagcg
    55501 gaaagctggg tggtgcggca ctggatgtgt tggagggcga ggatcagttt gtttataccg
    55561 attgctcgca gaaagtgctt gaccatccct ttttgtcgca gcttttaagg atgccgaatg
    55621 tgatcatcac accccatacg gcgtactaca ctgagcgtgt gctgcaggat actacagaaa
    55681 aaacaatcag gaattgtctc aattttgaaa ggagtttaca gcatgaatag aataaaagtc
    55741 gcaatcatct tcggcggttg ctcggaggaa catgatgtgt cggtaaaagc cgcaatagaa
    55801 attgctgcga acattgatac ggaaaaattc gatccgcact acatcggaat tacaaaaaac
    55861 ggtgtatgga agctatgcaa gaagccatgt acggaatggg aagccgacag tctccccgcc
    55921 atactctccc cggataggaa aacgcatggg ctgcttgtca tgaaagaaag cgaatacgaa
    55981 acacggcgta ttgatgtggc tttcccggtt ttgcatggca aatgcgggga ggatggtgcg
    56041 atacaggggc tgtttgtatt gtctggtatc ccctatgtgg gctgtgatat tcaaagctcc
    56101 gcagcttgca tggacaaatc actggcctac attcttacaa aaaatgcggg catcgccgtt
    56161 cccgaatttc aaatgattga taaaggtgac aagccggagg cgggtgcgct tacctaccct
    56221 gtctttgtga agccggcacg gtcaggttcg tcctttggcg taaccaaagt aaacggtacg
    56281 gaagaactta acgctgcgat agaagcggca ggacaatatg atggaaaaat cttaattgag
    56341 caagcgattt cgggctgtga ggtcgggtgt gcggtcatgg ggaacgagga tgatttgatt
    56401 gtcggcgaag tggatcaaat ccggctgagc cacggtatct tccgcatcca tcaggaaaac
    56461 gagccggaaa aaggctcaga aaatgcgatg attacagttc ccgcagacat tccggtcgag
    56521 gaacgaaatc gggtgcagga aacggcaaag aaagtatatc gggtgcttgg atgcagaggg
    56581 cttgcccgtg ttgatctttt tttgcaggag gatggcggca tcgttctaaa tgaggtcaat
    56641 accctgcccg gttttacatc gtacagccgc tacccacgta tgatggccgc cgcaggaatc
    56701 acgcttcctg cactgattga cagcctgatt acattggcgt taaagaggtg ataagtatgg
    56761 aaaatggttt tttgttttta gatgaaatgt tgcatggcgt ccgttgggat gccaagtacg
    56821 ccacatggga taacttcaca ggaaaaccgg tggatgggta tgaggtgaat cgcatcatcg
    56881 gcacaaaggc cgtggcgttt gctctgcgcg aggcacaaat ccatgcggct gcgcttggct
    56941 atggcttgct tttatgggat ggatatcggc caagaactgc ggtggactgc ttcctgcgtt
    57001 gggcagcgca accggaggac aatctcacaa aagaaaaatt ttaccccaat atagagcgag
    57061 ccgagttgat tacaaagggt tatgtggcct cacaatccag ccatagccgt ggaagcgcaa
    57121 ttgatcttac gctctaccac ctggatacag gggaacttgt ttcaatggga agtaacttcg
    57181 attttatgga cgaacggtcg catcatacag caaaagggat aggggatgca gaggcacaaa
    57241 atcgaagatg cttgcgtaaa atcatggaaa gcagcggatt tcagtcttat cgctttgaat
    57301 ggtggcacta taagttgatt gatgagccat accccgatac ctattttaat tttgccgttt
    57361 cataatgaaa gtattggttt tgtatcagat tttggatttg ataaaatcta tgctggtcat
    57421 ggacaatctt ttactggaaa gtggttaaag catcttttgt aatacattgc aaattatagt
    57481 attcaatctg cccagcggca gaaaagaaaa aaaccgctgt tgggcagacc tttcccacgc
    57541 ccattttgta ttttgctggc ggcggttttg agaaatcaag gccgccgttc ttttctggca
    57601 gcaaaaggtg tggcggccca taggaggatg ccgctatgct gttttgctat tgccatgctc
    57661 taatccactt ccaccactca aaaaaagcct tgcccctc
//
LOCUS       DANJMI010000016        55969 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000016 DANJMI010000000
VERSION     DANJMI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.016"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(70..615)
                     /locus_tag="PWU81_001136"
     CDS             complement(70..615)
                     /locus_tag="PWU81_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL1109855.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(627..1319)
                     /locus_tag="PWU81_001137"
     CDS             complement(627..1319)
                     /locus_tag="PWU81_001137"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL1109856.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1338..1622)
                     /locus_tag="PWU81_001138"
     CDS             complement(1338..1622)
                     /locus_tag="PWU81_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109857.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1635..2198)
                     /locus_tag="PWU81_001139"
     CDS             complement(1635..2198)
                     /locus_tag="PWU81_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL1109858.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2426..3088
                     /locus_tag="PWU81_001140"
     CDS             2426..3088
                     /locus_tag="PWU81_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDL1109859.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3212..4408
                     /locus_tag="PWU81_001141"
     CDS             3212..4408
                     /locus_tag="PWU81_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDL1109860.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4509..5885)
                     /locus_tag="PWU81_001142"
     CDS             complement(4509..5885)
                     /locus_tag="PWU81_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1109861.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5922..6789)
                     /locus_tag="PWU81_001143"
                     /pseudo
     CDS             complement(5922..6789)
                     /locus_tag="PWU81_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
     gene            complement(6878..7903)
                     /locus_tag="PWU81_001144"
     CDS             complement(6878..7903)
                     /locus_tag="PWU81_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL1109862.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7921..8595)
                     /locus_tag="PWU81_001145"
     CDS             complement(7921..8595)
                     /locus_tag="PWU81_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1109863.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8779..9672)
                     /locus_tag="PWU81_001146"
     CDS             complement(8779..9672)
                     /locus_tag="PWU81_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL1109864.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9746..11443)
                     /locus_tag="PWU81_001147"
     CDS             complement(9746..11443)
                     /locus_tag="PWU81_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL1109865.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11436..12749)
                     /locus_tag="PWU81_001148"
     CDS             complement(11436..12749)
                     /locus_tag="PWU81_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL1109866.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12749..16786)
                     /locus_tag="PWU81_001149"
     CDS             complement(12749..16786)
                     /locus_tag="PWU81_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL1109867.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16799..17257)
                     /locus_tag="PWU81_001150"
     CDS             complement(16799..17257)
                     /locus_tag="PWU81_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL1109868.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17555..18073)
                     /locus_tag="PWU81_001151"
     CDS             complement(17555..18073)
                     /locus_tag="PWU81_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL1109869.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18075..18692)
                     /locus_tag="PWU81_001152"
     CDS             complement(18075..18692)
                     /locus_tag="PWU81_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDL1109870.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18689..19543)
                     /locus_tag="PWU81_001153"
     CDS             complement(18689..19543)
                     /locus_tag="PWU81_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1109871.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19540..20451)
                     /locus_tag="PWU81_001154"
     CDS             complement(19540..20451)
                     /locus_tag="PWU81_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1109872.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20492..21826)
                     /locus_tag="PWU81_001155"
     CDS             complement(20492..21826)
                     /locus_tag="PWU81_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1109873.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            22034..22864
                     /locus_tag="PWU81_001156"
     CDS             22034..22864
                     /locus_tag="PWU81_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDL1109874.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVDGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23108..23426)
                     /locus_tag="PWU81_001157"
                     /pseudo
     CDS             complement(23108..23426)
                     /locus_tag="PWU81_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23548..23742)
                     /locus_tag="PWU81_001158"
     CDS             complement(23548..23742)
                     /locus_tag="PWU81_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109875.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24247..24432)
                     /locus_tag="PWU81_001159"
     CDS             complement(24247..24432)
                     /locus_tag="PWU81_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109876.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25282..25623)
                     /locus_tag="PWU81_001160"
     CDS             complement(25282..25623)
                     /locus_tag="PWU81_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL1109877.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25623..25853)
                     /locus_tag="PWU81_001161"
     CDS             complement(25623..25853)
                     /locus_tag="PWU81_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL1109878.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            26140..26478
                     /locus_tag="PWU81_001162"
     CDS             26140..26478
                     /locus_tag="PWU81_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109879.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26683..26922)
                     /locus_tag="PWU81_001163"
     CDS             complement(26683..26922)
                     /locus_tag="PWU81_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL1109880.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(26898..27245)
                     /locus_tag="PWU81_001164"
     CDS             complement(26898..27245)
                     /locus_tag="PWU81_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL1109881.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(27977..28558)
                     /locus_tag="PWU81_001165"
     CDS             complement(27977..28558)
                     /locus_tag="PWU81_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1109882.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(28741..29367)
                     /locus_tag="PWU81_001166"
     CDS             complement(28741..29367)
                     /locus_tag="PWU81_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL1109883.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(29389..30720)
                     /locus_tag="PWU81_001167"
     CDS             complement(29389..30720)
                     /locus_tag="PWU81_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDL1109884.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(30875..31276)
                     /locus_tag="PWU81_001168"
     CDS             complement(30875..31276)
                     /locus_tag="PWU81_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL1109885.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(31362..31568)
                     /locus_tag="PWU81_001169"
     CDS             complement(31362..31568)
                     /locus_tag="PWU81_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109886.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(31663..33165)
                     /locus_tag="PWU81_001170"
     CDS             complement(31663..33165)
                     /locus_tag="PWU81_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDL1109887.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(33183..33638)
                     /gene="argR"
                     /locus_tag="PWU81_001171"
     CDS             complement(33183..33638)
                     /gene="argR"
                     /locus_tag="PWU81_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL1109888.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(33736..34353)
                     /locus_tag="PWU81_001172"
     CDS             complement(33736..34353)
                     /locus_tag="PWU81_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDL1109889.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            34574..35947
                     /locus_tag="PWU81_001173"
     CDS             34574..35947
                     /locus_tag="PWU81_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1109890.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            36022..37134
                     /locus_tag="PWU81_001174"
     CDS             36022..37134
                     /locus_tag="PWU81_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL1109891.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(37206..38843)
                     /locus_tag="PWU81_001175"
     CDS             complement(37206..38843)
                     /locus_tag="PWU81_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL1109892.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(39011..39619)
                     /locus_tag="PWU81_001176"
     CDS             complement(39011..39619)
                     /locus_tag="PWU81_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL1109893.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(40003..41154)
                     /gene="queG"
                     /locus_tag="PWU81_001177"
     CDS             complement(40003..41154)
                     /gene="queG"
                     /locus_tag="PWU81_001177"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDL1109894.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(41173..41925)
                     /locus_tag="PWU81_001178"
     CDS             complement(41173..41925)
                     /locus_tag="PWU81_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109895.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(42066..43015)
                     /locus_tag="PWU81_001179"
                     /pseudo
     CDS             complement(42066..43015)
                     /locus_tag="PWU81_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            complement(43016..43855)
                     /locus_tag="PWU81_001180"
     CDS             complement(43016..43855)
                     /locus_tag="PWU81_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL1109896.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(43852..44598)
                     /locus_tag="PWU81_001181"
     CDS             complement(43852..44598)
                     /locus_tag="PWU81_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL1109897.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            44836..46206
                     /locus_tag="PWU81_001182"
     CDS             44836..46206
                     /locus_tag="PWU81_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1109898.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(46261..47157)
                     /locus_tag="PWU81_001183"
     CDS             complement(46261..47157)
                     /locus_tag="PWU81_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL1109899.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(47271..47441)
                     /locus_tag="PWU81_001184"
     CDS             complement(47271..47441)
                     /locus_tag="PWU81_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109900.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(47438..48115)
                     /locus_tag="PWU81_001185"
     CDS             complement(47438..48115)
                     /locus_tag="PWU81_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL1109901.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(48206..49111)
                     /locus_tag="PWU81_001186"
     CDS             complement(48206..49111)
                     /locus_tag="PWU81_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL1109902.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            49286..50710
                     /locus_tag="PWU81_001187"
     CDS             49286..50710
                     /locus_tag="PWU81_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1109903.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            50756..51787
                     /locus_tag="PWU81_001188"
     CDS             50756..51787
                     /locus_tag="PWU81_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL1109904.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            51794..52981
                     /locus_tag="PWU81_001189"
     CDS             51794..52981
                     /locus_tag="PWU81_001189"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL1109905.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            53137..54519
                     /locus_tag="PWU81_001190"
     CDS             53137..54519
                     /locus_tag="PWU81_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL1109906.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            54646..54876
                     /locus_tag="PWU81_001191"
     CDS             54646..54876
                     /locus_tag="PWU81_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109907.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(55076..55744)
                     /locus_tag="PWU81_001192"
     CDS             complement(55076..55744)
                     /locus_tag="PWU81_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDL1109908.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT        16099 a        11698 c         9917 g        18255 t
ORIGIN      
        1 aaagtcgttt gttttatgct cttcttttat tattttgtgc tttttatttt atcaaatcac
       61 tttgtttttt tataaaattt ctgcgcctag tgtggactca aaatgtctca atgcccattc
      121 atgtccaacg ggatcgaaac ttgccacgga atctttgaaa atatggagtt tgtatcctag
      181 attatacgca tcgactgctg tatgcaacac acagatatct gtacatacgc cggtcaaata
      241 aatatctgtg atctggcgtt ctcgcaaacg gatgtctaga tctgttccac taaaagcgga
      301 ataatgccgt ttgtctatcc aataaacatt ttcctcttgc tcatagtctt ggtataaact
      361 ttttaaagag ccgtacaatt cccgtccacc cgttcctatc acattatgcg gaggaaataa
      421 gcggttctcc ggatgaaagg aatcttttgg atcatgcgca tcaatggcaa aaacgacaaa
      481 atccttttga tcaataaact tctttgtatg agcagtcagc tttttttcaa ttacttgtcc
      541 tgctgtccca gtagtcagct ttccatcctc tgcaacaaaa tcatatgtgt agtcaatcga
      601 gatcaatgcg ttcatcttag tcctccttat acgagatgtt tgacaaattc gatcaccatt
      661 tctgctgatc gtttgcctgc gtcttcgata aattcgtcaa agctttgtgt cgccgagtga
      721 tctgctgtgt cgctcattgc acgtacgatc aaaaatggga tattgaactg acgagctgtt
      781 tgtccaacag cggctccttc catttcgcaa gctaatgctt cagggaaatt cgttaagatt
      841 tctttgattt tatctggcga gtcgacaaat gtatcacctg taacgatcaa tccttctttt
      901 gcgttcaaat tcgttgcttt tgctgcttta accatttcac tgcgcaaata tgtgcttgct
      961 tcataatata gaggcattcc tggtaattgt cctgggttgt agccaaatcc tgttgcatct
     1021 gcatcaaaat aagctacttt gtctgatata acgatgtcac ctacttgtaa gccttcaccg
     1081 attccgccag ctgaacctgt attgatcacc atgttcacgc cgtattgttg gatcaacaga
     1141 cttgtggtaa tagatgctaa gacttttcct ataccggaac gtacgacgat cacttcgtgg
     1201 ttacctaatg aacctgaaat aaataaagcg cctgcgcgtt cccatgataa tggttcgctt
     1261 aggttctctc tcaaaatttt tacttcttct tccattgcgc cgataatacc gattttcatc
     1321 tttttaattc cttctttcta tataaatgcg attgctaaaa aaaccacaca taacattagg
     1381 ataacaataa taagagattt catcaaaaaa gagttccatt tggtcgtctt ttcccgttcg
     1441 ctgatccttg ttttcgtgat tggtttattc tttttgcttt ctttacgata aaagctggca
     1501 atcttttttt cttcttgctg ataagctgtt tctgcttttc gctgtttttt taatgactcg
     1561 cttgcttgtg cttgttgtcg cttgcgaagt tcgcttcgcg taataagcgg tcctttggcc
     1621 ataagccgat ccctctactt tcctttggtc aatagttccc agtattgaat cgcataaatc
     1681 gtctttgcat cacaaatcaa ctgatctttc atcgcttgtt gcgcctcttc aagagttaga
     1741 tgatacaact ccagtacttc gtcttcatct tgtgctaaag gattctcgac cttctctacc
     1801 ccttgtgcat gataaatatg aaggacttca tttgcaaaac ctggtgaaag atacatcgaa
     1861 gtaagatggc tcagcgactt cgcacgatag ccagtttctt cttctaattc tctagctgct
     1921 gtcatttcgg gattttgtcc ttctcctgga tcgatttttc cagctggaat ctccaaaatc
     1981 actttttcta aaggttttcg aaattgtttg acaagaagca gacgatcttg ttcgtcaaaa
     2041 gcaatgatcc cgacaccgcc aggatgaaat accagttctc tcttcgaagt tcctccatct
     2101 ggcaaacgaa cttcgtctac agcaacatca ataatttttc cgctatagat ttctttgcga
     2161 ctgattgttt tttcttcaaa ttgctcattg ttcagcatat cttctcctgc tttctttgta
     2221 atcataactg cttttttttc atttttcaat ttttctcaaa aactccttat tagtttacca
     2281 tagataaacc ttagattttc tctaaatata gaaaatcctt ctttagtccg atactctttc
     2341 tagtagtccg tatgctaaaa taagtatgta atcgaataac gaaaagaatt caatactcaa
     2401 cgataaatga catggagggt ttattatgat aaaacgaaat tggccgtggc ttctagcatt
     2461 ggtcttgcta tttctctttg tccgaaacaa tagtttttct tttgttctag gacttttatt
     2521 gataggtgta gttttagttt tgtggggact ctttggttcg cgcaggaaaa agggaaaaca
     2581 agaagaaatt cctgcactga ctgacgaact tgaaactcac tatgaaaaaa gcggtatgac
     2641 agcgaatgaa atttcatttt ttcgacaaac aatgaatcaa acaaaaaatg agatcaatca
     2701 attgcaaatg aatatgcagc agacagccaa acttaaagca atcgatctcc gccatgatcc
     2761 agtcaaagca gcgaaagctt tattcaaaga attggtgaaa gaaccaaacc gactgcatga
     2821 agcaagtcag tttctttata cgcatctgcc gaatctagtt gatttgacaa ataagtataa
     2881 tgagatcaat gaccatgaga tcaaaaacaa acagacctat gaaaaactag aagaaagcgc
     2941 gcagatcatc gaccagctgt cagctttaat cgcacaagat tatcaaaaat tcgttgctga
     3001 ggatctagat gatatcgacg tagagatatc tgttgcgaaa cagagcttaa agcgtgataa
     3061 taaatatgaa ccagaacaaa aggattaact cttttgttca taaaagaaga ctcggaacat
     3121 tcagatgatg tctggctgtt tcgagtactt tttctaaagt agagacattc atgtaagaat
     3181 cgagaacaga ttcaacaagg aggaattttt tatggatcat aaaccgactg aaacaaaaga
     3241 tgttacacca gttaacgata cacttgaaga tttattgaac aacccgtttt caactccaac
     3301 agatacgctg accacttcac aacaagcgga gattgatcag ctccaagagc aacagacagc
     3361 tgctcgttta attgacaaac taccggcgga acgccaagaa caagctaaac agctagcagc
     3421 aaaaatcgat gctaatgatg cacagtctgt catcagctac ggatcggctg cccaagcaaa
     3481 attaagtgaa ttctcacaat cgatgttgaa tcatgtgcaa gcacaagata ttggtcctgt
     3541 aggagattcc ttaactgaac tgatgtatcg tctccaagaa gccaatcctg acgaactgcg
     3601 tgccggagaa gggaacatct tccagcgtgt cttcgggaag gtcaaacaat cgatctatga
     3661 agtgacagca aaatatcaaa agattggtgc acaaattgac aagatttcag tgaaattaga
     3721 taaagaaaaa gacggtctgc taaaagataa tctgatgtta gaacagcttt accagaaaaa
     3781 caaagactac tttgatgcac tgaatattta tatcgctgct ggagaattga aaatggaaga
     3841 acttcaaaca acgatcattc cagaagcgat gaaaagagca gaagaaactg gcgatcagat
     3901 ggatgttcag attgccaatg attacacgca attcctagat cgtctggata aacgtacgca
     3961 tgatcttcgc ttagctagac agatcacgat ccagcaagca ccacaaatcc gtttgatcca
     4021 aaacacaaat caggccttgg ctgaaaagat ccaagcatct atcgctacag ccattccgct
     4081 gtggaaaaac caagtcgtga tcgcattgac cttgcttcgt caaaaagatg cagtaactgc
     4141 tcaacgccaa gtctctgaaa caacaaacga cttattgaag aaaaattcag aaatgctgaa
     4201 aatctcagcc atcgaaacag cgaaagaaaa tgaacgtgga atcgtggata tcgagaccct
     4261 tcaaaccact caaaatgatt tgatcgaaac aatccaagag acattgcgta tccaaaaaga
     4321 aggaaaagaa aaacgtcgtc atgcagaagt agagcttgga cacatggaag aagatctgaa
     4381 acaaaaactg ttggatttaa cccaatagat cacctttcgc ttgttactaa aacaaacgcc
     4441 caacaacagt ttattacaac tgttgatggg cgtttttctt atctttcttc taacttctcc
     4501 attggagtct atttctcatt catcttgctg tgtccatagc catagccaaa ataaatgatc
     4561 agtccaataa ccattacgat accgaacacg atccatgtca cagtttgcag acgaagcatc
     4621 aatacaatac aagtcaaaat ggagacgatt ggcaatagcg gaacgaatgg cactttgaat
     4681 tctccagctt ttggtttgcc aaacattttt ctcaacttca gtaagcctag actaacaagc
     4741 atcaaatagg cgagcgccat gatattcgtt agttccgcta atacattcaa tgggaaaaat
     4801 ccggcaaaga atacagctgc cacaccagcg gcataagtcg cttttttcgg tgtacgatgc
     4861 ttttgatcga tttctgtcat aaacttagga agtaagccat ccttgctgac agcaaacagt
     4921 aaacgagcaa gtgaatacat catcgagatc gttaccgtca gtagcgttaa aattgcccct
     4981 acagcgatca ccgcgcccac tgttccatgt cctacatacc tcattgcaaa agccactggg
     5041 tcattgactc ctagttcagt atacggaacg attcctgtca agatcagcgt cacgatgatg
     5101 taaaggacag tagcaatcag tatcgaacca atgattcctc ttggcacatc tttttgcggg
     5161 tttttgactt cctcggctgc cattgcaacg gcatcaaatc ccaagaaagc aaaaaacaca
     5221 agagctgcac catctagcac accggaaaaa ccaaaaggca tgaatggctg ccagttatca
     5281 ggtttaacga aaaatattcc tacaatgata aacaacgcga tgataccaaa tttgacatat
     5341 accatggcat tattcaaacg taatgctttc ttggcttcaa gcgacacgat gtaagtgacg
     5401 aatatcacta cgagcaccgc aatcaagtca atgtatgtac cattttccgg attgtacccg
     5461 ccagttaatg cttgtggaag ttcggcccct aaacttttca aaaaaccttg catatagcca
     5521 gaccatccgg aagcaacaga ggaaaccgct agtaaaaatt ccccgatcaa caaccaccct
     5581 gtcagccagg cagcaaattc accaaataca acatacagat aggcatatgg cccaccaatc
     5641 actggcactc gcgaagcaaa ttctgcgaaa cttagtccag acaaaatgac aacgattgca
     5701 gctaagacaa atgaaagtga cagtgctgga ccagcatttt gattggccgc aacccctgtc
     5761 accacaaata ttcccgtacc gatgatcgca ccaattccaa gcatgatcaa atcagacgtt
     5821 tttagttctt ttttcatctc actcgtatga ttgagattga tctctttctt acgaaacaaa
     5881 tccattgaag ttcctcccca ttttaggttg gaaaacagcg attaaactgt tttcatatac
     5941 cctaaaaggg ataccaccta aaatgactaa aaggcaacca acaataacat aaatgagctc
     6001 ttttttcgtt tttctttcgc caaggatgaa aatcccacct aaagtagaga taatgatccc
     6061 catttgtgaa agtgagaagc caacagctaa accaagtgat ttgactgtta gaagcatgca
     6121 gatgtttcct aagccccata ataatccaca gatcatgttt ttccacacaa actgatcgac
     6181 tttaacttta cggaaagcaa agaagcttgc gcctaagatc attccgatac tttgaggtaa
     6241 gataattgac atcgcatcca aattcgccca gttgacgatg atggtatata caccataacc
     6301 aatcgtagag aagatcaatg ctctaaaacc cttaccaaaa tcaagcattt tattatctgt
     6361 atctattttt ccttcgtcat cttgtcgggc agtcaaatac gcaccgctta caagaagagc
     6421 taaagcaata ataccgtata cataatcttt cgtttgtgtc cattcatgga agaaaacggc
     6481 tcccgcaatc gtattcaaaa ttaattgaaa accagtagac aatggtagcc ctacagaaac
     6541 acccatgtac ttcattccat ggaactgtcc attttgtcca acactccaag ctaagccgga
     6601 taaaaatccg atcaagaaaa tccaaggggt gattacagga ctaacaacga aaaagacaac
     6661 taaagaaaac agaaatgcac cgatcgtcat tcctagtgtt tgttgattgg catcgccacc
     6721 aattttacca cttactaaac caatactacc ccatgccaac atagggacta atgctattaa
     6781 aatttccata accaatctcc ttttatttca catgataaga aacttatcag cgaaaacgtt
     6841 aacaacacaa gcactttttt tcgtttctaa cgattttcta acgtttttca ttataaaaat
     6901 ttaagattgg gaaaaataaa gtcattgttg tcccttttcc ttcagttgat tcagccgtca
     6961 aatggatacc taatttttcg gccagatttt ttgctaaata aagtcctaat cctgttgaat
     7021 gtgttttgct aagtcgacca tttctcccag taaatccctt atcaaagatc cgtttcaagt
     7081 cttccgctgg gataccgatc cctgaatctt taactgaaag gctgactcct tgcgcttcct
     7141 tttctatgct cacaatgatg tttccgtgat ccggcgtata tttgatggca ttactaagca
     7201 attggttgaa gataaaagcg atccatttag catcggtcaa aacagtatga tcctcaccaa
     7261 cgatctcgta gcgaatattt ttttggataa aataatttgc ctgactgcgt atcacaggct
     7321 gaatgattcc ttttagtcca tattcttgga tgaggtagtc ccgcgagaaa ctgtcgagtc
     7381 ttgcataata caaaacttgt tctacgtatt catcgatttt tgataattca ttttctagta
     7441 atgtatactt ttgatcgtca atatcgaact cgatcgaacg aactagcaat ttagaagcgg
     7501 aaagtggcac tttgatttca tggacccatg agtcaatata atctttttgg tcttgttgat
     7561 tactgattgc tgattgcatt acttcttggt gttcacggat cagctgattg atatattctt
     7621 ctaccagttt ttcttcttct gtcctcccac cactcaaata attttgcaaa ggggaagttt
     7681 tttgtatgtc ggctaatttt ccccaccatc ttctcttgct caagtaacca cccagcagat
     7741 aaaaaatcag aaagacgccc tcaatcaaag ctaaataagc aattgtgctc aaatcaactt
     7801 tgatgtttgg cgccagccac atcacaaaga tcgtcaatgc aataaaaaac agccatccca
     7861 agatcaatgt ccactgatct ttcaaatatt taaaaaaatt catcttaacc gctcatcctt
     7921 tcagggaacg atatatccct gtcctacttt tgtttcgata tatccttgga tacctgcctg
     7981 ttcgattttt cggcgtaacc gattgatgtt gactgtcaat gtattgtcat ctacaaatcg
     8041 ttcgtcatcc cacagtgctc gcagcaattt ttcccgactt aagatctttc cattgtgctt
     8101 catcaaaata aataatagac gatattcatt tttgctcaaa tcaatgatct ctccgttgat
     8161 ttccatactt ccgttgtcaa cattcaacgt aatgccattg tggctcatca tttcactaga
     8221 taattctgtg tagttgtaag aacggcgaag taatgcattg atttttgcaa tcagtacatc
     8281 gatttggaaa tgttttgtca caaaatcatc tgcccccatg ttcatagcca tgatcatatc
     8341 catattcgta ttcctgctgg agataaaaat gatcggtact ttcgatactt cacgtatttt
     8401 ttgacaccaa taatagccat ccaaaacggg aagattaata tctagtaata caagctgtgg
     8461 ttcttctctt tcaaagtctg agaacacttg attgaaatcg gtcgttccaa aggtttcaaa
     8521 ttgccatttc tcaagttcct ccatgatcaa ttgtcgaatg atctcttcgt cttcgactac
     8581 catgattttt gccataaaaa atccctccat ctttcttctt gctactacaa tatcaaagaa
     8641 gcccttgtga aacaagttgg cttacctgga aaatgtcatt ttagcaaacg ttaaaaaaaa
     8701 ctggaaatat gcgatttaat cgcgtttgtc tcttccaagc agcttaaatt tattatgtta
     8761 ttcttaataa ttggtctatt atctgttatt tttctttcgt tgtttttgtc ttttaaattt
     8821 tcgataacac caataaccga aaagcgttag cactagcaat atacctagaa ggagtataag
     8881 taatcgataa agatggtatt tttttgtctg ttctatcttt gaagagactt cttctgtaaa
     8941 gggaactcgt cttcctgtca ctagtaatcg gtgagtgttc acggtgtaag gtgtacaagt
     9001 gataagtgtg actagatcct gcttttctct aattgctaat gtatttgttt ttgttggaag
     9061 gacaacaatt ttctcaacga cttcataagc gagcatccga ccatacactt caagatagaa
     9121 tcggtcatct tgctccattt cttcaagatc cgtaaagagt tttttctcag caataccgct
     9181 atgggcggta atcacggaat gtgtactctc tcctccagtt ggaaaagagg tgccttgcag
     9241 gactgtcgct cctctatcta acaaaagatc attcgtttca tcaaataaag gcaagctaac
     9301 attgattttc ggaatataga tggctccgat catatgttgt tcgtaatatt cttttcctgg
     9361 attttggaca tttctaaccg cttgctcaaa aggatctttg acttgtccca ttcctgggat
     9421 tactgatgta tgagcaagcg tctgattttt cttttcatgc actgctaatt gttgctcatg
     9481 tttttcttct ttatcgcgtt caagctgctt ttgataattc tcgatccttc tttgatccaa
     9541 ataattgctg agagcatctg ctaaaaatgg atatgtaaaa actgcagctc cggcaagaaa
     9601 catcactgcc ataaataatt gtaataaaag actgatttgg gtttgaggtc tattacctat
     9661 tttctttttc attttcttca ccttcttatt taaaaaaact gcagcaacct ttataaaacc
     9721 gattgctgca gcttattttt gttgcttata cttcagactt cattttagat ttacggtacc
     9781 agctgtacgc tccaatcatc aaacttattc cgattagtaa gaatgccaag agcccgttcc
     9841 caccagtagc aggaagtgac ccgcgtttca tattcggtac tctttctggc cctggatttt
     9901 ctactcctac gatctgaatt tcttcattat agcttccata agagatgata aattcagtaa
     9961 aggctgcctc acctgttgga agtacataac cttctggcgc catggtctct ttcatttgat
    10021 aggttccata atctaaccct tgaacctcaa agtatccatt tccatctgcg acaaattctg
    10081 ttgcttgtcc gcttgttgcc cactcaattc tatctgctcc tgattcataa acagctaaag
    10141 gcgactcccc tttataaaat attgcgtatt cagaacggtc ttcattccac aattcgaatc
    10201 ttgcatcttt aagcaattca ctgcttgatg catcatattt ttcaaacttt cttccacctg
    10261 ttgtgacttt ctctggcggc tctactcctt cttcataatc aaattcatca tttcgtccga
    10321 tttcaataac cgctgtgttg ttatggaaat catccgcttc tagttctgtg gaaaccttca
    10381 aattatacgt gatcgttaaa tctcctccac gtgcatctgc taatctacct aaagtatcta
    10441 ctttggctac tccattttct tctgtcaaaa tcgtcacgac aaacccgttg cttactactt
    10501 ctactatata atcttgtcct tttgttaaaa tcgtctcgcc ggctcgcact tctatttcct
    10561 gatcataata agttagacca actgtaggcg tatctgtgat tttgtaattt ttgataacag
    10621 cgcttccgtt ttcaaggaca gaatcgatga aataaggaat aggtgctgtg atcgtgtaag
    10681 aagccgtttt tcctctttct agatcaatat aagaaatcgt ttcttgttct ccagcttcat
    10741 taataatcgt tacttgtcta aggtcgcttt cctctacatt catctgttta tctgcttctc
    10801 tgatttcgtt tttcggatac aaatgcacat ctgtattgta tgtgtctttc catgtagaac
    10861 catcccacac gccatccacc atatcaggca tcatcactgg catcataaca accattggag
    10921 acgcgattct ggaaatatga gctggcgaat aagtttctaa gaaaagatag actttatctc
    10981 tagaagaagg tgcttcttcc gtcacttgga cggtagagaa taccgcttct ccagcttcat
    11041 tcgttgtttg tttgtcaata cttgtcagtc gatattttaa aaaccaactt tctgtgtgcc
    11101 gtttttgaat ccattcaatc gcttcggcag aattaagacc gtcttctcgt tccggtgtta
    11161 aaggattatc tcgaatcaaa tcgtaatata cttccgttac gtcaaacaaa tcaaaatcga
    11221 ctcctggaat attttcttca ccaaaatccg tagaaaccaa tccgctattc ggtcgttctt
    11281 ccggaacaga tgagaaacct tttttgtgta atgtaatcgt cacttcactt ggtttttgtg
    11341 cgacttcttc ggctttgact agttttccgt tcccgaaaat ccctattccc gcagtaacga
    11401 gtaacagtac actcattccc gtgacaatca tttttttatt catttttttc tccccgttct
    11461 ttttttatta ataaaacagc acctaacaat aaaagaattc ctagtaaaga aatcaacgtt
    11521 cttgcttcat tcgtttgcgg gaatacggac cattttcctg attgacctgg attgcttgga
    11581 ccactcggct ctttcggttt gtccgttgtt tcttttacgg tctcgttata aacacgtggt
    11641 gttgcggatt cataggcaga gactggaact ttcccggatt ctaattcagc catctcctgt
    11701 ccgttaacta ctacatattg tggttggcct tttgcatcca cccaaaattc agggatgatg
    11761 acctcgattc tcttacttgc ttcatccatc tcgtaacctt ccacaccttt taattcttca
    11821 aaataatagg tgccagaagg caagaaacgt tgtcctgtcg taacaaggcc ctctttatcc
    11881 gaaatgaatt tgcttacatt tttgtcattc aaaggatcat tgtctgctgg tttgatccat
    11941 tgatttttca agtcattagc aggagccata tctaaataat atttcccccc ttctatcatc
    12001 tgatatagag caaaaacagc accttctaat ggttttcctt tttcagttgt tcctctttga
    12061 cgtccatact tgaagaagta tggatctctt agataaccga catttttagg atagatatga
    12121 atctcttgca gttcctcggt tgattctgtc gggtgataaa ttggcaaaat agcagcaatt
    12181 gggatagctg tctgatcgat atcaacatcc aatccaactt ctggatcaat cgattcttcg
    12241 aaaatcaaat aaactgcatc ccgatcttgg tttttcttag gtgctttgat acgtgcaatc
    12301 ccgtctccta atccgccaac cgtatctttc ccagttgtta ccgaagtccc tatttggaca
    12361 agcctttctt ctgtgattag ctgacggata ttgtttctgt tcgtttcagc gatttgctgg
    12421 acgaattctt taggagtcat gttttcagca gctttttttt catgataata atcggtcatg
    12481 tcgtaaatgg caaatgtcgc tccatttagt ggcaccgtct gtgaaatgat atctgctgtt
    12541 tcccctgctt cctcagagat agccaagcca tcattttcat ggtattcaaa ttgatcgtta
    12601 taatcgatat ctcgaatcat ccgtttatgc aacacgaatt ccacgacatc ttcccctgat
    12661 tcttctgccg acatcgcatg tggaagaagg actgctgcaa gcggtagtaa aacaagtaat
    12721 tgaagcatca aacacaacag ctttttcatt accttgctcc ctccttttta gattgtctga
    12781 caaacaaacc ataggctgct cctgcaagcg ccatgaagaa tgcagcaagc aggatataga
    12841 ataatcgtcc ggatcctcct gtagcaggca acggattttc aggatcgttc ccgacactaa
    12901 gctgtaatct gattcgattt gtctccaaaa gttcatattc taactcttgc tgtagatcag
    12961 gcgtctctgc ttcttcgttt ggataatacg aaatctcaac ttttagtgta cccgcttctt
    13021 ttcctggaac tacccgatca ttttctatgt caggttcttc tcgataacta atttctaaaa
    13081 taaagtaacc tggaagtttt ctatagctgt caggtgactt cacttccttg atccgataga
    13141 ccccttccaa caagtctgaa gcattgaata gggaagaatc gtttggatct ggtgccaagc
    13201 ctccctcgat gatttcatac tctcctgcac tattttgttt ctcaattgaa aattctgcgc
    13261 cttcaagacg tcggctattc atactgtcaa ttttcaaaag ttccaaatcg attggcgcta
    13321 gttcatcttc taaggttact tgtagtacac catcgatgat ttcatagcct tccggcaagg
    13381 gagcagtttc gtcttcgatg accgtatctt caatgataaa cttaccttct tcattcagtg
    13441 caaactggaa cacttgatca gatagccgat acccatttgg tgcgacagat tccgagacct
    13501 catattcacc aatagacaga tgcagaggtt ggtcgtaatt taaatcgaca tacttttcta
    13561 ctcctacggc acgagaatag aagtatccga tcccaggcaa acgtttcgat tcttctggtt
    13621 ctaacggatt accagtgtat gctcgataca gcgggaagga agaatccccc actttcctga
    13681 tatcgaaacc tgcaccatct aatgtatggc cttcatggtc taattttcta actcggatct
    13741 cccgataatc aaacgtattg taagcagtga atgctaccca taaacgatcc tcttcatctg
    13801 ttgtatacgt gagatattgc caattttctt cacctgtttg cttgagttgg atagtcccta
    13861 tagcattatc gtaaagtcgc aagatccatg tgtcattttc atcaggttct ttgaaacctt
    13921 ctgggttctg gctctcaatg attttgtatt ctgcatagtt ggtagtttct tcactatcct
    13981 tataaacaaa tgaatctggc tctccgattg gatgaataac tttctcacca tccacgatta
    14041 catatttccc gtcttcatct actgaataga acgtgagtct ccctttctct tcctctactt
    14101 cgttcacacc agaaagccct ttcgctactg gatatccgat ttccagtccg ttaccatctg
    14161 tttcataaag ttcaaactct gcgccattga ttggcatatt actgccttca attagtttat
    14221 tgatattcat gtggattggc ttgaattcat ttcgcatttc cagtttgaca tacaactcat
    14281 catactctgt gccttctatg ttaggctctt gtacaacaga aagcttgtga tattctcctt
    14341 cataatagga attcggatct ttaccttccg gtccgccgac tctcgaacca gatgtatatt
    14401 catctacaag ctcgtagtga cctaacatct catcttcgtt ggctgcatat tttttgattt
    14461 ggaattgata cgtctcatct actaactgat aacgtggtgg tccagcgatc tctctgatcc
    14521 aaaatatgtc gctgactgct ccagtttcat ctcctaaaat cggcatcttc gtttcaaaga
    14581 tcgctattcc gtccgtggta gttttacttg tttcgattgg ttcggcttcc ccactctctg
    14641 gattttggtc atataatgca aattcgactc cagccaacgg ctcgccattt tgacccttct
    14701 tgctgacttg gaaattacgg cctttatgtt cgtaacgaat ggaagaagat gggaaataca
    14761 tccgtccagt agtgatatca ttctcataat cctccaggtt ctgaatatag gccatattgt
    14821 tcactgtgca aaaatttccg tcttgagctc ccgcatcaag ttttacttgg tacttgatcc
    14881 tgaccatttc cccttcacct aaagaaatcg gagaaaattt ataagcttgg ttttcccgtc
    14941 cgacatacgt tgtcggagtc gccactactt ctggtggggc ttgtccatga cgttcttcat
    15001 cccatatttc tggctcttgg tacttcatgc cgttttctcc tggatctaga tggtaaaact
    15061 gaatatctgc cgatctgcca gctccaaatg gtgagacatt aaaaccagtt tcatcataaa
    15121 gtgtggcttg ctggatcgtt ctcttgaagg tagaagagat atcctccaag gctgtaacaa
    15181 tgtcattctt gttgttcgca ttgtaatatc gcggtgttac gccatcttct tctaaatcag
    15241 caatattttt caatacattt cgtccaatag catctagtcg ttgtctcccg ctaacgtttc
    15301 catcgattcc taaaccaatg gagaagatac ttggcgggtt ctcttgatac ttgtttttca
    15361 gccaatgatg ataagcaact gtgttgatcg cagatgactt ttcttgcgaa gtaggactgc
    15421 cattcgtatt gttattagaa gaaccatcac caacccagtg agtcgcttta ttatctcctt
    15481 cttttacctc tccataacga tagtgtacat ttacttcatc ttctggacgg ttaaagtcag
    15541 atgcataggt aactggatga gtaaaccctg cattagaagt attcccagaa aactcctcaa
    15601 aatttctgaa caaaacgtca ttatcttcag caatcttatc ttccatcaca gtccagttgc
    15661 tccaagctcc attagtgctc gtacgataac ttgactgaat cggtgcataa ctgaaggtcg
    15721 gtgtcccatc tcccacaacg attaggattt tttctggatt tctattttct ccgccattgt
    15781 ctttatacaa tgtttcatag ccatttttaa gccctagtgt aagcggagtt cctccgattg
    15841 gtgtacgatt taatgctgta ttctcacttc ccgtcaaacg attgatatca tttgtcaatg
    15901 gaaagatgtc cgaactgatt tgctcatggt tattgatagc tgtactatta tggtaaaaat
    15961 tcaccatacc gattcgaata tccagactcg ggtcagagag cagatcttta ctcatttcgt
    16021 ttactgcctc aatcaacgca gcatttttag tttgactatt ggtcccagcg gttaattcac
    16081 tcatactcgc tgacttatct agaacaaaaa cgatatcgat tggactgatt tcctttttct
    16141 cgcccccgat gacatctaga tagacattgt aaagggttgt tgcatcttca ggatgtagat
    16201 cagatgctgt gctgcttggc agaacaggtt ctacccattt tttgatgtac gccccgttat
    16261 aatccaagta actgttttca aaattccgtt catttcccca aagctgaacg gtttgagctg
    16321 gaacgtttct atcttcttcg cctgcagttg atccattatt agtagcatag tcgtaatttc
    16381 gccagttcgc tcccccgttt gtataatcac tatcatcgat caagttctct ccgtcatatg
    16441 gcgagccttt gtctccttga tagcgttcat tgctttgtgt ccaatttcct aacacttttg
    16501 tatagtcatt cgttgtaaac ttcgggtagc gatgctgttc gttttcaact tctccgctag
    16561 gatagatata ttcatagggg tcttgcgtag ctgttggccc aagacgctcc aaaataccgt
    16621 tgaataactg gcgttgaatg cgctggagtg cttgaccgct gggtacgatt ggttcgtcca
    16681 gcttttccat ctcattttct tcgctacgtt gaatttcagc ggaatcttct gtatcagttt
    16741 cattttcttg taattcttgg ttatctattc tttcatcaac caccacttga agaggtagtt
    16801 acccttcgtt cacatcgggg atcttttctg tttcaaaaat caatgtgccg gtttctgctt
    16861 cttttgaata aacttttccg acataccatt cttgaccatc gatcgtctcg acttttaggt
    16921 cttctccatc ttcatcatag gaggatagac ctgttccacg aacgtttttt acggtaccaa
    16981 tcccggcggc tgccgcatcg attgctaaac gtattctacc ttcattggca ctttcctttt
    17041 tttcaaaagc cagatgccat tcaagggttt cttctttttc aatatacttc ccttcaattt
    17101 ttgcatagtc ctcatctaag aatacctcat cctcctttgt ttctgttgac tcagcagcta
    17161 catccagggt cgtcatatga tagactgact ggaaatatgg tccaaatagc aaactgacag
    17221 ttactaactg caaaagcttc aattttaacc ctttcacatc cttatactcc tttcaaaaat
    17281 atttgtcttc tttcacaaca atcaacaaaa caatgcccat ctaattataa tttaatttaa
    17341 ttttaatttt aaatcaaatg atatcctttt tagttaaaaa acaccaatta tacaacattt
    17401 attattaaaa ttaaattata aaattttata acgttctcat ttaacaaaac ttaagttatt
    17461 aagacaaatg aataaacaac tacgctttcc tcgaaacaaa aaaataaagc acaaatgcgc
    17521 atttacgcac tcatgcttta ttcgtacaat taattcagat aacggcagcg cataggatgc
    17581 caataacact tcctaaaata gctcctccta aaatgtcagt aggataatgt aaaagtaaaa
    17641 aaatcctcga ataagcgatc aaacaagcaa ataaaaaggc taatattcct gcaactggac
    17701 ttaattcgaa caacgcagct actgataaaa aggctagcgc tgtatgtcct gaaggaaaag
    17761 aagaagtctt cggtttactt gtgtaaagga caagccgacg atttttttca taaggtcttt
    17821 tccgtcttgt cgtttttttg ataaaaagca aaccaacaaa aaaatagatc aaactgccga
    17881 taagaatggc tagaccatat tctctagtat caggtatcaa gactaaaaca gcagcaagga
    17941 gcaaccaaat aaaaccacca ttagagatat tactgataaa tttcaaaaaa ttgatttttc
    18001 gactatccgt tttccgttgc ttggcgaact tgttgataat actatcatcg attttagcaa
    18061 tcaaacgcaa catgttattc ctccttttta agaacctttt cttgcgtacg atggataaaa
    18121 agcaggactt gaaaataaaa caagaaactg aaacctaata aagcaaaata gattggtcct
    18181 gcaaaccaga atgcgacaag aaggatcaac gtgcctgcca aatagaaaag cgcgcttttg
    18241 aaatcattaa aaaacaagaa aaatgcagta atccaagaag cttctttttg ttccagttga
    18301 agcaagattt tgtaactaaa gagaagcgcg cttaatagaa gcaaaaacaa cgtagtatac
    18361 atcaatgtat agccaattcc cccaccccaa tagcggaaaa tctgataatc gatcaataga
    18421 agaagaaaat aaacacttgc taaactatct tgctttccat aaagagagaa gttctcacga
    18481 aactttttcc agaatagtga accgagcttt ttctcttcat tttttttgta atgataaatc
    18541 atatacagaa ctgttcgact tgcagttcct aatgtgaaaa caggcaagac gcacaggaac
    18601 catagtagct gtaaatggat aagacgtgga atccattcaa tgatttctaa gaaataatca
    18661 ttggcttttt taccaatcga tcgattcatc atccttttga acctcctgtc aaatccatcc
    18721 cttcaatgaa ataacgttgt gcaaaaaaga acagcaaaat cgtcgggatc aagacaagtg
    18781 ttgctcctgc catcagataa ttccattgtg tcgttgcttg gttctgaaaa acttgcaagc
    18841 caatctgtaa cgtgtatttt tcttgatctt ggatatataa cagaggacct aagaaatcat
    18901 tccaagctgc gttgaaggaa ttgatcgcaa tggtaatcaa ggcaggtttc gtcaatggaa
    18961 gcatcaaatg actccaaata tacaaatgat tcgctccgtc gatttcagca gcttcgatca
    19021 attcattatt gatcgataag taaaattgac gcatcagaaa tatattgaac gcattgccga
    19081 agaacgatgg aacgatcagg ggcaaataag tacctaccca gccgatcttg gagaataata
    19141 catattgagg gatcatcgtt acaaaccccg ggatcatcat cgtagaaaga accaaagcaa
    19201 acatcagctt tttcccagga aaatcaagct tcgcaaatcc ataagcaata aaagaatttg
    19261 aaaggacatt accgatcact actagcacag tgatgaataa tgtatttctt gcatagcgcc
    19321 aaaaaggaaa ggcggcaatc gtatccagat agttttccca tacgattttt tctgggaaaa
    19381 aggtcggagg ataacgaacg atttcttcca ttggtttcaa tgaagtaaac accatccaaa
    19441 gcaatggaat gatcaaaatg attcctatta ctgtcatgag gacataactt agtatttttt
    19501 ttgtttgccg attcacactg gtctttttga aataatccat catttgccgt cacctccaga
    19561 ataatagacc cacttaggtg caagcttcaa attgacaaac gtcagaacca acacgatcag
    19621 aaacaggatc attgacatag ccatcgcata acccatatca aaagctacga acgccttgtt
    19681 ccacatatgc agattataaa aaagcaaaga atttgcaggt ccgcctgttc catttttcgt
    19741 catgacataa gcttcttgaa aaatttggaa agaaccgatc gtagaggtga tcacatcgta
    19801 aaaaataata ggagtgatta atggcaaagt aatacttttg aactttcccc aaacgcctgc
    19861 tccatcgatt tctgctgatt catataaaga tctaggaaca ttttgtaatg tagctaagta
    19921 aagtagcatt gctcctccag aactccatat tttcatcaaa atcaacgcag gtttcgtcca
    19981 ttgtggatca aacagccaag ctggtccagg gatatggaac cagcccagaa atgtgttgat
    20041 caatcctgca gatggagaaa gcagctgcat ccataagatg tagactgcta ctcctgataa
    20101 gactgccggt aaatagaaag ctgtacggaa tacactgatt cctttgattt tttgattgag
    20161 tactgtggct aaaaacacac tgaaaatcgt cgtcagcggt acattgaaga tgacgaaata
    20221 aacggtgttg tataacgatt tccaaaataa atcatcgtga acgaacattc ttttgaaatt
    20281 tgctagacca atgaaattca tcttggatgt caaattataa tcggtgaaac taccaataac
    20341 tgaaaaaagc ataggcccta gcataaatac caagaacccg ataataaaag gcgagataaa
    20401 taaataggcc catcttgctt cttttttctt tcggatagat aaacgcttca aattttcttg
    20461 cctcctctgt acctagacag tcaaaaatgg attattgatt ttgcttgact aagttttcta
    20521 ctgctttttg tgcatctgcc aatccttctt ttggtgattt tgttcctaaa agaatctggt
    20581 caatctgttc attcactaat gtcgtgtaat ccggagcatc taaaggaact ggtgtgatca
    20641 ccgtattttt aaagttttcg tccgccattt gatagatcat ttttccttta tccgttaggt
    20701 tgtcattatt cactaaattc tcattggctg tcttgctcgc catgatatca aaactttttt
    20761 cgccgaattt ttcttgtact tctggagaag aaagatattt gatgaattca taagaggcct
    20821 caggatcttt tgctccgtat ggtacttcca agacgaatcc gccaccccac gaccaatggc
    20881 ctgaaccttc ttctttttct gggagctgca tcacgccaaa atcaaaatca tccggtgcat
    20941 tttccatcaa actagagtaa taattgatat tttgggcacg catggctact aatccggaaa
    21001 taaatggatc agatacacca gacccaaatt cggcttctaa tttattgatc gtatttttcc
    21061 cataataatc ttgccagtct tggatccact ccaaggcttc cactttattt ttagtatcaa
    21121 tcttgacttt ttcatctttg tcaaaccaac tagtcccgtt atctgcgttt agcgcccata
    21181 catcagcccc taaattccat aatggataaa aaccgatgcg tgtaaattct tttccttctt
    21241 ttttatctag tttacgtgct gtttcttcta attcatccca agttgtcggt agatcttgtt
    21301 cactgattcc agcttcttta aataactttt tattataaaa aatgacttgt gtatctgtat
    21361 tgaatgggat cgcataagga tcaccttcat attctactgt atcccaaagc tgcggataaa
    21421 aatccttaga agtgtctttt tctacatatt tggagagatt agttgcttgc tgtgcttctg
    21481 ctctttgggc aacggaattt atgtcctgta caataacatc gggtgcattt ccagctgtta
    21541 ttgcagataa agatttagtc cagatgtcac cccatggctg atacttataa tttacatgga
    21601 tctcgttttg tgattgattg aaatcctcaa tgatttcttc gatagcttct tttcgtgcac
    21661 cggatcccca aaaggaccag aaatcaatat tttttcttcc atcacttgtt ttttcagaag
    21721 aatttgctgc acttgaactg gcatcgcttg cattacttga tccgcatcct cctaataaaa
    21781 acaatgctga acttgccagc aaccctgtaa acaataattt tttcatattt tacctccaaa
    21841 ttttaatgta accgatgccg gttttaatat aacgatatca ttttgttctg aaaataacaa
    21901 tttataaggt atgattctaa aaaaattatt aatgatacca atactttata taattcgcga
    21961 gcgctttctt tgaatttttt aatctttcgt agcagaatga gaatgtatta aaaatagatt
    22021 aggaggaatt ttgatgttta gacaccagaa agaattgcag tttgaagcaa aaccagatcg
    22081 tccgaatcca ttaatcgcga aatatctgca ggagctgatc ggcggccaat atggcgagat
    22141 gagtgtagct atgcagtacc tgttccaagg ctggagctgc cgaggagaag aaaaatataa
    22201 agatatgctg atggacattg caacagaaga aatcggccac gtggaaatgt tggcgacaat
    22261 ggttgctcgt ttattagagg gtgcgcctgt tgaagatcaa gaagcagcga tgcaaaaaga
    22321 tccaacaatc ggtgccatta tctctggaat gaatccacag cacgcgatcg tagatggctt
    22381 aggacctcgt cctgctgaca gcgtgggcaa tccatggagc ggtgcctata tcattgcgag
    22441 tggcaacttg ttagcagact ttagagctaa tttaaatgcc gaatcacaag gaagacttca
    22501 agtagcacgt atttacgaaa tgatcgatga tcgtggcgta aaagatatgc tgtctgttct
    22561 ccttgcaaga gatagctatc atcaaaatct atgggaatgt gcagtgaaag aattagaaga
    22621 aaaagaaggt tcaatcttag tcccaagtac tttcccgcgt gacaaagaac gaaaagacat
    22681 tgcttatgac ttctataact tctcagaggg agaccaaagt agtgaaggtt cttgggctag
    22741 tggcaaagca ctggatggcg aaggcgaata tcaatggttg aaagaaccta agatcgaagg
    22801 cgctgcacca aaattagaac cagtaacacc aaaaatgtat gggacacctc cattcaagaa
    22861 ataaatatct acccgtagaa acacccgttt tttgttccac acgctttgtg agcaaaactg
    22921 actaaagtca ttaatcataa ccacccttca aaagggtggt tttctctaga gctataagct
    22981 caaatcaccg gccagcgcct aaagacgctg gctttcatat tttatgttca agccatattg
    23041 cctttgccag ttttgcccac ctcttaaaag gtttatgtac tactccttag cccttaaaag
    23101 gatcgtctta ctctcgtaca ctcagcttat ctaacgcact atcatgtttt tcttgttctt
    23161 gaatgtactt tttgatggtc gcttcatttt gtcctacttt tgtcacatag tatccttcca
    23221 cccaaaagtg tcgataccca aatttatatt ttagatttgc acaatctctt gatgatctcc
    23281 tgaatattgg ctcgatattg attataaatt atttttcttc tatactttgg tgtgaataca
    23341 atgtggtatt tacacatcta ctttgtacgg gctaaactat ttccctttat tgctccaccc
    23401 gcatagcaag tggttttgta tttcaatcgc tcggcgcgct caattaggtc taaagacaat
    23461 aaatctaaaa acccgctgct tcactgacag aaacaacggg ttttcagaaa aaaatgaaat
    23521 gaaaaagatc tcttacggca atctatttta tttttttgat tttttataaa tggaaaatgc
    23581 ttcaagaagt ccaagaaata aagccgtata aaaacaagaa cctatgaaat ctttatttat
    23641 tagatattca acagaaatcc cgataacact agccaacaat atatttatca gaagtgtaaa
    23701 ataaggatgc tttttataga atagaactag tgttgttttc atagactctc ctttcttcat
    23761 atttccaatt aatcacaaat tcacggcatt actagaaaaa ttaaaggggc gcagaagtgg
    23821 aaaaatactc ttcgactttc cctacaaacc aggccatctt gtgtttaata actataccaa
    23881 atctaaggta cctctaaatc taaattccat ttgtggcggt gacctccaat ttttaactct
    23941 tctatctccc atccagtata cataaccttg atacttcgac cacttatata tcgaacacac
    24001 aaatacctat ccattgcagg agtactacct aacttagttg ctacttgcat ctaaataaca
    24061 gctgaaaaga taggacttac tcttagtaaa gtatctgaac tattaatttt taaactatca
    24121 tttttatcct caactacatg cacatctgtt gcaacttcac tttctgtctc atccagataa
    24181 atgattaagg aatcgggaat tgcaatatct tcagctgacg ctggaatagt tgagctaaat
    24241 tagtcatcat atctttatta taaaataacc aacagcaaaa aatacaatga aaaacaaact
    24301 tggaactaat gtatcattaa taaatgttga aaaattcagc aactcattac tgtatgattt
    24361 tagcagccca accaacatat accctataaa agctaataag atggttagga atcctttaga
    24421 atttttactc attcatagac acttccttaa ataactttta ttatagaagg tattgtaaat
    24481 aataatattg acaaaaagtg actcaaaata aaaaaaacct tacattatac tggaatgaac
    24541 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
    24601 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
    24661 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
    24721 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
    24781 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
    24841 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
    24901 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
    24961 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
    25021 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
    25081 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
    25141 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
    25201 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
    25261 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
    25321 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
    25381 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
    25441 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
    25501 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
    25561 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
    25621 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
    25681 tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca
    25741 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
    25801 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
    25861 actcccttaa taaaagtata ctatataaca ctaagtaacg caaaattccc ctttgtaaac
    25921 tatctagaaa attgttttgt ttaaagtata tcttattgga tataaataga aataaattgt
    25981 agccagttca ttttttcttt aactaaaaaa ataatccaag aaacattgaa cctatggatc
    26041 caaaagctac ccaaatacta tagctaaccc cccgctacct tcaattttgc agataaaacg
    26101 ctttgggaga aaatttcaaa gacaacaggc catcctatca tgcccgatta taaacaaaaa
    26161 gctggctatt ccttttgctc tgacaaattt caattgggaa agttcacgaa actagcaggt
    26221 gaagaagttt caactgttcg gatcaaagaa tgcccgcttc aaatagaaac attggttcaa
    26281 cgaatttcaa ctagacaaga ctttgctatt gtagaacgtg agatcgtagc tatttttgta
    26341 gaagaaggga ttttacatga tccaactcat atcaatgtgg ataaatggca acctcttatc
    26401 tataaattca gagaatatac aactgccaac cagtcgttaa ctttgaattt tagattccaa
    26461 gaattcaaag agtcataatg ataaacatcg ttaggtataa ataaaaaaga ctcgcagcac
    26521 gagtaaaaaa caaatagaag ctatacatta gaaaggtcga ctgtgaccct atagatgcag
    26581 tatatgcagc ttatgagcat ttcttctgtt ctctttgttt ttgggatctg tgctgaagtc
    26641 tttttcgttt tttgccgctc tttctttgaa aaatcgatta tgttatacct tctccattac
    26701 taattcaggc atctgctgag taatgatttt tcgcgcaaaa gaaatatctg ttacccaagg
    26761 ttcgatttgt tcttcagtca agacaagcgg cattcggtca tggattggtg cgactgtgcg
    26821 gttaggttca gtagtcatta tgattgattc tgccattttt tcatcctgat ggctacgata
    26881 aaaaccgcca atatacacta ttttttcctt atttgaaaac ataaatttgt ctttttcttc
    26941 attccactca ttgggaaaac acaacgattt tgcaaaaatg gttgatgaaa ggtctttttt
    27001 tcttctactg tctctgcacg ggcattaatc atcaattgtc cttttttaaa tccggtaaat
    27061 ccccacttca tgatttccgg agtgaggatg ttttcttcct cttttcttaa cgtcacaaca
    27121 agatttgacg gaaaaacttc tccagctgct aattctgctg cctgcttcgg ttgtttcgtc
    27181 tgggcttctc gataataatt ctgaaggagc tctgactcta gatcaaaaaa ataacgacca
    27241 cacatcctaa tccctcccaa tctttttttc tatcatacac tgcaacaaga agttcgcacc
    27301 aacaaaacgc tttcaatttt tgtagaacta aaaaagttgt gacaagtgac aaactctcca
    27361 gtgaatggta ttctcctgcc aactcctcgg tcacaagtca caatattcaa caaacatgag
    27421 aagctgcttt ttgaaattgt tccttgtgac tcggagtgaa acggcaggct tacgacctct
    27481 gggtaaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgta
    27541 gaaagctatt ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct
    27601 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
    27661 tatgagcagc tatttttatt tggctgttct tattacttga tgcagggcag ctttttcaca
    27721 acctctccac taaacggtgt tcaatcagct aaccttcggt attagctcaa aaaacggcaa
    27781 aatatgagga actattttgc cgtttgtttc tctaatactc aggtcaaagc gctgatttca
    27841 caacctctaa tacacggtgt tccagaagta gcttcttcga aaaataagcc gaaatttcac
    27901 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg
    27961 tcacaacctc taattattaa agaacctttt gcgaagttaa caatctttct ttgatcaatt
    28021 gaaaattctg cctaagtagt gatagatcct gtgtgacttt caacaataag atacttccct
    28081 ggatttcagc aatcaacgct ttggcttgac ggtctgcttc ttcatcggac agtttttctt
    28141 cagaaagtgc caggatattt tgtttcgtat acttgaccca ttgattgact aattctagta
    28201 gcaacagacg gtattcttca tccactgtac tcaactcaat aatcaaatta cctacaggac
    28261 agccatgata agtgatctgt gtctcatgat cagcgaaaat ccagtccaac atttcttcta
    28321 atgcttcttc tggctgtttt ccagcattta agatgtcttc gaacaattct gtctgccata
    28381 cgtccatatg cgcatggatc accgctagac aaacttcttt ttttgaagaa aagtaataat
    28441 aaaattgacc ttttcctgta tcagaagctg ctagaatatc tcggattgac gtttcatgat
    28501 agcctttttt ataaataaga tctttggtcg cttccaagat cttttccttt gttttcaatg
    28561 atattctcct ttttcatttc tcatagtcct atattataca gtaaattcat cattttttct
    28621 tcttaatcgt ttaaaaaaga ctgtgccaaa ttatctgaca cagccctcac tataaaactg
    28681 ttctcatata gctagatgtt cattgattca agaactgcga ggacaaaagc aattcctatc
    28741 ttatgatagt tgctctaata cttcctctgt tgtcatcact tgatctgcaa taaagccaaa
    28801 gttcgtcaac gctgcatcca ttgcttcttg gccaggagca ccaattgcat ctgtcgctac
    28861 cacaacattg aatccactct ctaccaattc acgcatatgg gaatcgctac acaagtttcc
    28921 gttcattcca ccaagaatta ctgtgtcgat tccatttttt ctcaattgga gcgccaaatc
    28981 attgctttct ggtccaaaga ttttatgagg agagctgatg atcgtatttt catccaaata
    29041 aggtttcaat tcttcgataa aatcggctcc tgaaccttct tccactgatt catattgact
    29101 ttttctagca aacatctgct gctcaagcat catatgttct cctgcaccgc caaattgcca
    29161 tttatggtca tgtgggtaaa aataatgagg agaaataaag agtttgtatc ctttttcttt
    29221 tgcttgtttt accatttcaa tcatattttc gatcgtattg tatttttcta agatatcttt
    29281 tgtcaaacca aagccaccgc ctgctggttt caagaactcg acttgcgggt cagtgagaac
    29341 tactgctgta tttttattat tgaacatggt ggattctcca ttcgttattt attttaatac
    29401 atcatttaga gcttcactca ttgtcggatg agaatagatt tgatctctca ataacgtata
    29461 aggaagtttt ccattcatag ctagcgaaat caagttgatg acttcatgag aatcttccgc
    29521 atagatactt gcccctagaa tcaagtcagt ttcagggtcg actaaaactt tgaatacacc
    29581 tttaggatct tctaatactt gtgctttagg gacaccgctt gtcatatact tgaataattt
    29641 gtaattgacg ccttgtgctt tggcttgttt ttctgttaaa cctacattgg ataaagtcgg
    29701 agtgataaat acactgtaag gcacagcttt ccggttattc gttgttcgtt tattctcgcc
    29761 cttcaactga tccataatga ttcgataatc atctagagag atataagtga actgcaatcc
    29821 gcctttgacg tctccaatcg cccaaatgtt gtctgcatct gtacgaaggt attcatccac
    29881 agcgattgct ccgcgatctg ttagtttaac atctgtattt tctaatccaa gtgatgctgt
    29941 acttggtttt cttccagttg cagccaaaac ttttgacgct tggattgtta cttcttggcc
    30001 atcttctgta aaggtgacgg cagctgttga ttcttggtct gctacttgat ctacagatac
    30061 acctagatgg aattcgattc ccgcatttgt catgtcttct aaaatcattt gcgcgatatc
    30121 ttcatcttca cgtggaagaa attcagaatg ggcatcaaga acgacaactt tagatccgta
    30181 ttcattgaac attgatgcaa attccaagcc aatatatcca gccccgatga tcactaattt
    30241 ttccggtaat tgttcctgat ccatcgcttg cgtagaatca atcaagtagc gacttgtatt
    30301 caatcctttg attggtaaaa tgattggttc agaaccagta ttgatgaaaa tccgttcgcc
    30361 ttcaacaatc actttttcac cattattcgt cacttcgatt tgatggttag aaagaaaacg
    30421 tgctgtaccg tccatcactg tcccagttgc ctcatcgctg atcatatgat aatttttggc
    30481 gcgaagcatg cccgtcaatt tttctttccg agcaacagcc tctgtaaagt cgattcctct
    30541 ttctccattg aaaatcaatg atttagaagg aatacagccg atgttgatac atgttccgcc
    30601 atacatacgt gttgactttt caacgactaa aaccgattca ccttttgaag ctaacgcctt
    30661 cgctaaagtc ttaccgccct ttccaaaacc gatcacaata ttttgatatt tttccatcat
    30721 ttttccctcg cttctttttt acctaccgat tggtacaaaa atatgataac cgataattgt
    30781 ctattctcca aatcatctgc tttatttgtc aaaaaaaacc agacaaaaag cggtcctttc
    30841 acgtttttag tctggttcga tttcctttat tcttctaaac aatcgttact tctacatcga
    30901 tatttcctct agttgctttg gagtaagggc agaattcatg cgcatcttct gcaatttttt
    30961 gggcttcttc ttcgctgatg ccatcgatat gcacttctaa tactacaccg atctgatagc
    31021 cgccatcatc taaattataa agagagacac gtgcttttac tgtggactct gctgtcattc
    31081 ctgcttcttt gatcatatgt tgtaccgccc cattgaaaca agaagcataa cctgctgcaa
    31141 ataattgttc tggatttgtt ccttctgcat ggattcctgg aggagtgatt ttcatatgca
    31201 tcgagccatt tggtgcctcg acttctccat tgcgcccgcc atggttgatc attgctgttt
    31261 cgtatgcttt tttcattgta ctgctccttt cgattgacca tttgctactt ggctataatt
    31321 atagtgtatg acaagtaagg cgctttctac aaaaattctg cttattcgaa taccagaatg
    31381 ccgtcttcta ctatttttcc aaaaaacaaa aaaatactac tcgtgaacaa cagcgtcata
    31441 tttgttccaa aataaatagc aatcttttct acataatctt ttatatgatt gattttaaga
    31501 tacatatata ccagaaagtt gaccccataa accaatactg ttaaaaaaat aaccatattc
    31561 gttaccattt tttcctcctt ttgctggtct ccctaccagc actcgattta accataccac
    31621 tttttttgcc gtccaagcaa ctagcctttc aaaaagactg gtttattctg ctgttaatga
    31681 ttcaagatca gaaaaatgtc cgaataattt tgctaatttt actaacgcca acttgttctt
    31741 tccgaattca tcattttcta tctgtaatac tagtaacggc tcatgtaagc ttaaacggat
    31801 caaaaaccag ccgtttccgt atggctcttc tagatcatat cgaatacctt cttcattttc
    31861 cgggtcgaag ttcatccctt tttcttcttc caacagtcgc ttcatttctt ctaatatctt
    31921 gtttccttgt tcgcgtggtt catcgctgaa caattttaat cggatttctt gtacttctac
    31981 gggctgtttc aactctttga tttgttcatc taatgtgata ccttttcttt ttaattctgg
    32041 caaaagcatc aggatctttg cgatgacata tgcaccatca tccagaaaat agttttcctt
    32101 gaatgcagcg tgtccgcttg tttcgatggc tagctgtgta tctttcccaa gacggttaag
    32161 ttctaatgct ttattgatta catttcgata gccggaaata taacgaactt ggatgcctcc
    32221 tttttcttca atgaatcttt gtagatgaga agaagtaggg gaattcgtca cgattcttgt
    32281 tcctggatgc tcttttaata cgattgtact taatactgcg atcaaattat ttcgattgat
    32341 cactgttcca gaagcactga caactgcaga acgatccaca tctgtatcga aaatgatccc
    32401 aagatctgct tcatttgcta aaacagcctg cttgatactt tccatcgctt ctttattatc
    32461 aggatttggg atatgattag gaaatcggcc atctggttcc aaaaattggg aacctgttga
    32521 atccgcacct aagctattca atactttttc agcaaagaat ccaccagcac catttccggc
    32581 atcaacaatg attttccaac catttagtgg tttgctttct agtgttccaa tccccttttt
    32641 aatttttgtg actaaatctt gcgcataagg tgtcaaaagg tctgcttttt ctacgcttcc
    32701 agtttgttcg ctttttattt tttgctgctg tgataggata aaagcaatat cttctttttc
    32761 tgctcctcct tctgaggtaa agaattttat tccattataa taatagggaa gatggctagc
    32821 agtcagcata accgcagcat cgcacgagaa ctgttcaaat tgagtggaca taaacatagc
    32881 aggcgtagta gctaattcga aatcgatgac atgtatccct tgttctttca gactttggat
    32941 caacgcctct ttcagtgctg gtccgcttat cctactgtca tgaccaatcc caatcgtcca
    33001 ttttttattt ggatcggttg atttcttctt tttctttatc cagtttgcta gtccccagcc
    33061 gatctttttt accatgtctg tcgtcaagtt tacttcatat ccttcaactg gtaatgcgat
    33121 tcctcgaata tccgatccat tttgcagcgc tttcatatct ttcatataaa agtcctcctc
    33181 atttattccc tcattttttc cagcttttct gcaaatcgct gtgcttcttc ttctgagcgg
    33241 caaataataa tgaatgtatc tacaccggcc atcgttccga caacttcagg gtaagccact
    33301 tcatctagcc aattcgttac cacatcggca ccatttcgtt cagtcaatac gataaccatg
    33361 aactgaacag actggatacg caaaacctcg cgtctaattg ctgtacgcaa acgttcttca
    33421 tttaaagaaa cagatggctg gttaaataat gtatagcgta ctattttgtt ttcattttga
    33481 ctttttacta acttcatttc tcgaatgtct cgagaaatcg ttgcttgagt cacatcggcg
    33541 cctgcttcat gcagccgcgc aagcagctct tcctgtgtcc caataggttg ttctgtaatg
    33601 atctggcgga tcagtgcttg tcgctgtttc tttttcatat tagacacctc tcaatgttga
    33661 ttatagcaaa agataagtaa tatttcttca tattttggta ttttttactt ttattttttg
    33721 ttttcattca ggttgctaac ctttttctat tttaacacgc aaaatcgtct tcattttttc
    33781 aggtgctgat tttcttggat cgctgatgta aatctctttg tgttctttag aagctctttc
    33841 atagccttct tttgcgatga atgcttctaa tttctcaaac gttgctggtt cttcggcata
    33901 gggcccgacg tgtaagattt gggcacaaag accttcttcc atctttttga atactacttg
    33961 ttctttcaag tcttccggaa gtttgctaga acgctgaatg gcttcttgtg ctatcgcttc
    34021 tgtaacaaag tcaggctgtc ggatcatcaa ttgataagaa aaatgttctt ttttcatcac
    34081 tggttcatgc aaatactttt cttgcagtcc ccattgtcct tccagtggat agacagtata
    34141 catctgataa tcaggaatca gccaattttt tttaggcgac atgcgtattg catagctgac
    34201 tgcatataga caggctactc ttcgtttaaa gtcttcctga ttcgggtcgc cgatccctct
    34261 gatgctaaag aatgattttt taggaacctc tagtatttgc gggctttttt tcatactata
    34321 gctcttgtct ttacgccatt caattttttc catttttctt cctccgcgaa attccattta
    34381 gttatttatt atcatacctc tttttgagtg acttggctaa tcgtgttgac actatttttc
    34441 agtttgtgct agaataagtg aagtaaataa atcgtacaga taacacgttt cggcgtgtcg
    34501 agagagaggc ggtttctatt tttataatag aaatcactct ctttttttac gaaatttttt
    34561 agaggtgatg taaatgacaa aacaaaaaca gctgctttta gtacctgcag taattgcaac
    34621 agggatgatg tcttttgcgg gcgtattgat cgagacggcc atgaatgtaa ctttcccaac
    34681 tcttatcaga cagtttgatt taacgactgc acaagtccaa tgggtaacaa cgatttatct
    34741 tttgatgatt tcgatcattg ttccgctgtc tacttatttg aatcgcaatt ttagtctcaa
    34801 aaaactcttt ttagcttcaa atctgctttt cttggcaggt ataatgatcg atttcttctc
    34861 tcctaccttc atggtcctat tgtttggtcg attgctccaa ggtgcagcga caggtatcgc
    34921 tcttccgttg atgttccata ttatcttgac atttgccccc ctcaatcgac gaggcgcgat
    34981 gatgggtgtc ggtactttga caacggccat tgctccagca attggcccca cttatggagg
    35041 cattatgact tcacatttta catggaatca tatcttcctg tttcttgtgc ccgttttgtt
    35101 tctttctttg attatcggct ggtttgctat tcccgagatg gcagtacaaa aaagtggtta
    35161 tttggattgg ataagcgttt taggaatttg tcttttgttt agtggaagtc taacattttt
    35221 gagtacgttg ggaacagttt ccagctggat tgctttaatc atcggcggta ttggctggtt
    35281 ccttttttac tatcgaagta aaaaagtgga gcatccactg atccgactgt ctattctttc
    35341 aaataaaaca ttccgcttgt ttttgattgg cttcttagtt tatcagtttt tattacttgg
    35401 tgtatctttt gtcttgccga atttccttca gatcgttcaa ggaagtagtc cttttgtcgc
    35461 tggattagcg atgcttcccg gcgcagcggt tggtgctttg ctttctcctt tttccgggaa
    35521 aatgctggat caatatggtc cgaagaagcc aatttttgct gggctgattc tttctttgat
    35581 tggttggcta gcattattcc tactcatagg acacgcttca gtgggtgtat tgattgcttg
    35641 tcatgtgttt tatatgatcg gtatcggatt atcttacagc aatatgatga ccaccggaat
    35701 gaatgcactg aatgaagaac tacaaggaga cgggaacgca atgttcaata ccttgcagca
    35761 gttttctggc gctgtggcta cttcattagt agcggtcatt atcaattacg tccagcatca
    35821 tacttcccat aactatgaag tgtctactac attaggatca aaagcagcat taggcgtctt
    35881 gttgctgttg cttctcgtaa gctttgctcg attcgcctat tatctgttct ttgctaaaaa
    35941 agcgtagatt ccatatacgg caggtgttgc atctgccgtt tttttgtgtg aaaatggttg
    36001 tttgtaaaag gaggcttttt tatggaaaaa attgccatta tcggtggcgg gatcattggt
    36061 atgaccctag ccagttactt agatacatca aaattcaatg ttgctttatt tgatgaaggg
    36121 acaggacagg caactaaagc tagcgctggg attatctccc cttggttgtc gaaacgcaga
    36181 aacaaacaat ggtatcgtct ggctaaagac ggcgctgctt ttttccctaa acttataaaa
    36241 gatttccaac ttgatcaaac gatttacgca caatcaggga cacttttgct tcgacctgaa
    36301 aaagatttag ctgaattggc tgttcttgcc gagaaacgaa aagaagaagc gccagaaatt
    36361 ggaaacatcg aatggctttc acctgaacag acatctcaag cacttccgtt attgaaacca
    36421 gcagtttcac tgcgtatcag tggtggtggt cgtctggatg gaaaagcata tttagaacat
    36481 cttggtcagc gtttagcaca aaaacaaatg gagcagatcc aggcaaaagt cactttgcat
    36541 aagaagagcg attcttggct catcaaggga ccgtttggag aatacacagc tgatcgtgta
    36601 attttgacac ctggcccggc acttaagcct ttattacaag aattaggcta tgctgcagat
    36661 atacgtccgc aaaaagggca gctcttagca tttcagacat cttataagat gagtcaagac
    36721 tggcctgttg ctatgcttga cggagaagct gatttcattc ctttcatgga tggaaagatc
    36781 cttttaggtg ctacccatga aaacgatcaa ggctgggatt tggctcctac tagcgaagct
    36841 tataagggat tgactaagag tacagaacag ttcttgactg atccagatca gataaaaaac
    36901 tggccatacg agttccgtgt aggtacccgt gcttatacgt cagatttttc cccatttttc
    36961 ggcgtactgc cagatgatcc aagcttactg gctgctagcg gtctaggctc ttctggcttg
    37021 accacaggtc cttatatcgg ctatctatta gccaacttct taaatactgg tcattggcct
    37081 acggaagatt atcaaaagga catctccacc tatattacga aaaatccatc atagaaaaac
    37141 agctttttca agtaaggatc ccgagcgata ttgcgcatgg attacttctt gaaaaagctg
    37201 ttattttagt tcaatttctg aatcaaattc tccaaacttt cttttgcttc atctggcaag
    37261 agatcttttc ctatctgagc ttcaatgaat cgtaaacgaa tatcaagtcg ttgtttcacc
    37321 agatttttat aggacgtatc ctcaaaagaa acaggaaaat ctggtatagg taaatagtgg
    37381 atgtcttcaa atggaccaaa cgacttgaat tctgccgttt gttgcacgat tctttcaacg
    37441 attgacaatg tcgtttcagg tttgattttt ttatcatcga aatatccagt attcaggaca
    37501 tagcaggctg tttgctgttt caaaaacaac tctcgaaaag cttgatagtc ttccctaaga
    37561 ggatagacac ggaaaggatt agcaaatggt tcaatgacta gctcatctag ctgtcttttt
    37621 gtagtgatat tttccgcgtc tgatcgtttt gttgctaatg ttgcaccaaa agacgctgct
    37681 aaaactgggt cattgatctt gataattggc ggaagcgaat catctttcat gatccaaaaa
    37741 acagcatcga tcttttcgct aagatgatcg actcggtcgg gtgtcgcata acgtgatttg
    37801 actgtccgcc catttccgtt tcgtatatct cctgttacta ggactttttc tcctttactt
    37861 gtacatgtca cgcccacatt ctgacaagta aggaaagaag aaatggccgg atcatcagac
    37921 ggatagtctt gtgttttatc aaagtaagaa ggttccaagg cagtagcaaa accatttttt
    37981 cgagaaataa cgaaagcatc gtcatgcaag atcgaaacag agtgaccttc cgtatgtttt
    38041 gctaaggtaa tcgttgattt tccagaaccg gacaacccaa atacagccat cgtataggtc
    38101 tggtcattac tagcatattg tttcatccca ccgtggcagg caataaagcc attgcgattt
    38161 cccaatgccc aagcaagcgt caaagtcgct ttttttaatt ctccaaagta acgtaatcct
    38221 agcacagccg ctgtattatg gagagcatca aacaaaatga gtccatttgg aaatgtttca
    38281 tgtgtccatt cgggatcaca aaagatataa agatctccct catcaaagga aacagacttt
    38341 tgataagtct ctaagatctt cgaattcgct ggctgaaaat tcagcaaata agaatacaaa
    38401 ttgttctcaa atccttctgg aatcagcaaa tgactttgca cagaaaattc ttcagatagg
    38461 ccaacgatta cttttgtatg gtaaaactgg cgcttgcgac tttgatacac agcttctctt
    38521 aaaattttct cgtatttgct ttgttccttg tctaagtcct caaagatccg ccgagcactt
    38581 gctgttcttc ccacaatgct tccatcattg aatactagtg tcctaacatc agctggtaaa
    38641 tctagttctt cagcatgtga gattgttaaa tctgtgatga tcgttcctgg actccctttt
    38701 gctagttgat aagctgtctt cctatcaaaa acttcatgaa cattattacc gtaaaatgtc
    38761 gtttctacaa tcgatcgaat actggataac agtggatttc cacttgtgat ttcgtttaat
    38821 tcgagactct cttttgtact catggctcct cctcattccc taaacaaaaa aacgttttct
    38881 ttattataca ttattcgagt ttttatttgt actgctgcgc tgtgggaagc aaacgagtta
    38941 tttttcacac aaaaaaaccg agaacttgaa taaacaagtt tctcggtttc atcattttcc
    39001 ttttatcggc ttagtaccag ccgtttgcta accagaatgt tttagcagca tcccatgaac
    39061 cataacgaga tgttacatat tgttctgcta cacgttcttg attggcagct gaatagtcgc
    39121 cgtttaagta tgaagcatct aattgataac ggccgatgta acgtccgtta gtagctgtgt
    39181 atgaaccgcc tgattctttt tgtgcgatcc attcttttgc agagcttgaa gttggcgctg
    39241 ttgcagcagc tggggcagca ggagctactt caactgtttc aacaggtgca acgtatgtat
    39301 gttcttgcgt tgctgtttca gttgtagatg ctgtattttc tgttgttttg ttttctgttg
    39361 ttgctgaaga atcatttgaa gttgggattg ttaattgttc accaacataa atgcggttga
    39421 tgtttgcgat cgaatttttt tcagcaattg catcgatcat tgagttgtct cctgcaaatt
    39481 tttgtgagat cttagataat gaatcacctg attttactgt ataaacttca tcagcgtgag
    39541 catttgtagt tcctacaaac attgcagtag caccagcagc aaaagttgta cctaaaacaa
    39601 ttttcttaaa tgaattcata ttaaactcct ttttttctcc gttttttatt ctttcgacgc
    39661 tattaacttt atcatttaga aatatttcat aggtaaaaga aacatgacat ttaatgacaa
    39721 aataacgaat tatttaatat ttgtaacatt taaacaaata tttttttgat acaaaaccat
    39781 aaaagccttg ttgtataagc aaagtaatcg tttttctatg tttttttatt tttgaatgaa
    39841 aaacgcagat tctgtaacat tcactgcaat aatccataaa aaaaagcaaa aaaatttttg
    39901 atttttatca aattctctta aaaaaatgta aaaaatacaa aaaaaccagt aattgttaca
    39961 acaattactg gacaaaaact ttgaataatg ttaactattt ttttatttta actttctccg
    40021 caataatttc tgggcatgtt ccaattcgtt tataacgact tcttctgttt ctttagctaa
    40081 tatttcatct aatacatcaa gccatttttc cggttctttt gttcctagac gtccaatagc
    40141 ccaagctgct gttcctctga tgacagggcg gacatctgtt aaacattctg aaatctcagg
    40201 caatgccgtt cgatcaccta gattagctaa agcaatcaat gcgtttctct gcaaaggttt
    40261 cttcccacgc catgaaccag ctaaatgccc aaattgctgt ttgaagtctt tattagacat
    40321 cgaaagcatt ggctttaatt ttggatatat ttcgtctact tttggttcca tctcttcatg
    40381 aaaatgaaaa tcttttcctt gattgtaagg gcagactaac tggcagatat cacatccata
    40441 aatgacattt cgcatttttt tacgatattc ttccggcatc atcccttttg tctgagtttg
    40501 ataagacaaa catcgctggg cattcattct tccgtcacca agcaatgctt ttgtcggaca
    40561 agcagtcacg cagcgcatac agtcaccgca accaaattct cctggtttat caggctcaaa
    40621 tacaatatta gtaacgatct caccaagata gacaaatgaa ccgaactctt cagtgatcaa
    40681 caatccgttt cgtccaataa atcctaaacc agctcgctgt gctacagcga catcgatcag
    40741 ttctccagta tctacttgcg gagctagacg ccattcctct tctttttgcc atttttctgc
    40801 tcttgtttgg atgaattcga tcaatcggtt caaacggtct ctgagtatat catggtaatc
    40861 gatcccccaa gaagcacgag caaactgccc gcgtttttca tccctaggca cttcttcatg
    40921 gatcctagta ggatacgcaa gtgcaatgga aatgatcgat ttcggctgat caaatgtcat
    40981 ttctggataa agacgctcat cgatatttgg atgttcaaag ccagaagtat ggccagctgc
    41041 tttttgttct tccaacgatt cttttaatga atcaaaaggt tccgccgtgg tgaacccgat
    41101 tttgtcaatt cctaatcgtt tgctttcttg tatgatttct tcttttaatg tcatggtctt
    41161 cctcgcttgc tgttaacggc tgtataaccc ataatatagt ttttcttttt tcagctggac
    41221 aaactggatc tgctgatcgg caatcttctt ttcttccatc catttttttc ttgcagcaaa
    41281 tacttcccaa taatttgttg aaattaattt tcttttgtta tctaatgcat taaaaaaatt
    41341 caatgaaact ggttttgtac ttaacagttc atggagcctt acctcttccc atggcgaaaa
    41401 ctcagacaga tcgcgggaat acaatacttc ttcatagaga tttttttgct gtgtgtacgt
    41461 gaatgtttgc gggattttcc caggcctagc aatttctact agacgattat aaaacatatt
    41521 cattaatttg ctgttggcaa taaattgact tttataatag gccgactgga tctttttgat
    41581 tactccttct gctttgatga ctgaaaataa tgcaggccat gctttgacgg ttatttcgtc
    41641 gttttgttta actaatacgc caggtgtgaa gccgactgga gaaaggaaca caaaataacc
    41701 gataccggta ttttcatcta aaggctgaaa ttcatcaaac aaccatttgc ctatgtacag
    41761 ctcttcatct ggttctgttg tctcattttg atttgctatg aataatttag ctaattctgc
    41821 ttcaagaaat tgaaggtctt gatccttgtc ggtattttcc tgctggctga tcaatcgaaa
    41881 gacttcttct ttaatcgtat atttatcttt acgtgtaaaa ttcatagcca caccttcctt
    41941 ccttactcta actataacgg aatttcttca aaaaaggtat ctgtacgacg aaaaacagca
    42001 taattttttt tcatatctct tataaaaaat gcaaggtctg ctcctcaagc tagaccttgc
    42061 acttttcagc tttcttttgt ttgtgacaaa ccttcatgga tcgtttcaat attccatttc
    42121 atcatctgat agtaagaatc tccttgttct ccttcttttg ctatggaatc agtgaacagt
    42181 ttatcataga ttttcaaacc cgtttctttt gctacccgtt ccatgcttct tgagtctaca
    42241 ctggtttcca cgaataaaac cggtacttct tttgctctta tctgatcaat gatcgctttc
    42301 atttgatcag gcgtaccttg actttctgta ttgatctccc atatataagc tgctggcaaa
    42361 tcatatgctc gtgaaaaata cttgaatgcc ccttcacttg ttactaataa ttttttgttc
    42421 tcttcgatag aagcaaaact ttccttcgct tgtgtatcta attcttttag tttttccaca
    42481 tatgcttctg cattttttta tagagtgtcg cattttctgc atctttttca gagaatatac
    42541 gtgcgatttc ctccacatat tttattccgt tagataggtc tagccaagca tggggatctg
    42601 cttttgtatg ttcttcaccg ctagttaaat aaagaggttc tacatttttg ctaactgcaa
    42661 aataatcttt tccttcttct tttttagccg tttccatcaa gttatcgaac cagctgttac
    42721 ctgtttcaag attcaaaccg ttgtataaaa taacatctgc atcgcttgcc tttttgatgt
    42781 cttctggtag tacttcatat tcatgcggat ctgttccgac tggtacgata ctgtggatat
    42841 ctatttgatc tgttccgacc tcttttgcca tgtccgccaa gatcgaattg gtagccacga
    42901 ctgacaactt ttctttactt ccttcatccg cttggtcaga agcctgcttt ccgcaaccgg
    42961 ctaataccaa gaaactaact gccaagatta aaaagagtga tttttttgtt ttcatctatc
    43021 tgcttccttt ctgaaataaa attccttttg ttggagaaaa taaaaaagca aggataaaaa
    43081 atgcggtaca tgctaacacg atcgtagctc ctgatggcca attgtaacta taactgaaaa
    43141 acacaccgac aactgcactt agcataccga ttcctgcagc tgtaatgatc attttcagta
    43201 aatggttcgt tagcaagtaa gccgtggcgg ctggtgtgat caacatggca atcaccaaga
    43261 ttgttcctac tgtctgtaaa ctgactacag ccactaatgt caaaaagaac atcaaaagat
    43321 aatggatcag cgatgtgttc agcccataag cttttgccat tgtcggatcg aatgatgtga
    43381 tcttcaattc tttataaaag aaaaagacaa atatcaagac gattccacta acgagtgctg
    43441 tcagcaacaa atcgctttcc ctaacagcta agacattccc aaataaaata tgatacaaat
    43501 ctgtcgcact ctgggcatat gaaatcaaga taatccctaa tgcaaaaaag gagctaaaga
    43561 caattccaat cgctgtatca tttttcaatc ggctccgctg agtcacgaaa ccaatcactg
    43621 ctgctgttag catcccaaaa gcagtggctc caaagatgta actaaaacca aacatataag
    43681 aaatggctac acctggaaga actgcgtgag agatagcgtc tcccatcaaa gacatccctc
    43741 gtaaaataat aaaagaaccg atgactccag aagcaagtcc tacaagcatg gaagtgacca
    43801 aagcgtattg caggaattga taattcatta atccatcaat aaaggattgg atcatttttt
    43861 ctctcctcct tttactaaat gtctgatgat ctcaccatag gccaattgaa ggttcttcga
    43921 tgtaaagact tcttgtactg gaccggaagc gatcagctgt ttatttaaaa gaataacctc
    43981 atcgaaatat tcttctactt tatgaagatc atgatggacg atcaaaatcg tttttcctga
    44041 attcttcaat tcctgcaaga tgtcaaagat ctttcgttca cttaacgcat caatccctac
    44101 gaatggttca tctaaaaaga tccattctgc tccttgggct agagctcttg caataaaaac
    44161 tctctgaagc tgtccacccg atagttcgct gatctgtctt ttggcatatt cttccatccc
    44221 tacttttttc aaagcttgtc ttgcacgttc tttttctttt ttcccaggtc tttgtccaat
    44281 tcgtaaagat ggatatgttc ctaaaagtac aacgcctaat acatcaattg gaaaggaaag
    44341 atccaattca cttcgctgtt ccacgtaggc tattttttta cggactgagt ttaccgactg
    44401 tccagaaaag gtgactttac cagtagtagc tggtatcaac tctagcaaag atttcataaa
    44461 tgttgatttt cctgcaccgt ttggtccgat aataccggta attttttgtg gatttatcgt
    44521 caagttgata gttttcaaaa cagttttccc ttgataagcg gctgaaaggt cttgaattac
    44581 aatggccgta cgttccaaat cttacactcc tttttaggtt tacctaactt tttatttaaa
    44641 tatagtttaa ctaaaatgga aagcattttc aagtagaatg atgaaaaagt ttttttcgaa
    44701 ttttcctcgc attttatgaa aattttctgt agaatcacat caagagttca ataaggctaa
    44761 cctaataatg aaaaacatgt gttgaaacgt ttgattattt tttcttgttg actcaacaac
    44821 gaaaggagtt tttgtatgga agaagaaaag ttaactcgaa gtcttagtgc ccgtcatatc
    44881 caaatgatcg cactaggtgg aacgatcggt gtcggtctgt ttatgggtgc ctcctcaacc
    44941 atacgttgga cagggccctc agtgatggga gcctatgcga ttgcaggatt ctttttgtat
    45001 ttgattatgc gtgctttagg cgaaatgctt tacgttgatc cttcgactgg ttcttttgca
    45061 aattacgcga gcgagtacat ccatcctgtg gctggctact tgaccgcttg gagtaatatt
    45121 tttcaatata ttgtcgtagg aataagtgaa gtgattgctg taggatctta tatgaattat
    45181 tggtggcctg atttgccggc aattattccc ggaatcattg tcgtcttgtt tcttatgctg
    45241 gcaaatctta tttctgtcaa ggcgtttggc gaattagaat tttggttttc gatcatcaaa
    45301 gtcataacga tcattttgat gatcattgca ggtcttgggg taatcttatt tggatttggt
    45361 aatcatggtc aggcagtagg gatttcaaat ctttggaaaa atggtggatt cttcactgga
    45421 ggcgtcaaag gatttttctt tgcattatcg atcgttgttg cttcctatca aggaatcgaa
    45481 ctgatcggaa tgactgctgg tgaagcagag aatcctaaaa agacgatcat tgaagctgtc
    45541 cagtccacta tcggtcgaat cttgattttt tatattggtg ctatttttat cattgtgagt
    45601 atctatccgt ggaatcaatt gagtgagatt ggttcacctt ttgtacaaac tttttctaaa
    45661 gtcggcatta catttgctgc aggactgatt aattttgtcg tgattacagc ggcattatca
    45721 ggttgcaatt cagggatttt cagcgcaagt cgtatggtct atacacttgc cataaatgga
    45781 aagatgtcta aaaaatttct gaaactatct cgtcacggtg cgccttttta tcctgtcgta
    45841 gcaatctcag gtggaatctt aattggtctg atcttaaatt atcttctacc aatgttcatc
    45901 aaccagtcag aaaatatttt cgttttcgta tacagctcaa gtattttgcc tggtatggtt
    45961 ccttgggtag tgattttgat cagcgagatt cgcttcaaaa agaagcacgc agataaaatg
    46021 catgatcatc cttttaaaat gccactatct ccttatacga attatgtgac acttgctttt
    46081 ttagctatcg tattgatttt catgtttatc aatcctgaaa cgagaatctc attgattatt
    46141 ggactcgttt tcttgcttta catgacgatc cattactttg tacgcgaaaa aagaaccgga
    46201 aaataaaaaa agaacgaagc ttcaccttag tggaagcttc gttctttttt attgctttta
    46261 tcaattcaat tttagcgtct caccgggagt caggaaataa ttctccatgc tcatcccatt
    46321 cagcgacaag atcgtttcta ctggtacgcc agttcgttcg gctatttgat ttggaccttc
    46381 tccttccaaa actacggttg tcgcagcctg tgaagtcgcg ctgttggtat tagtttcaga
    46441 ggaatctgtt gtgtcttgtg ttgttcgagc ttgcatttca gaagcttcta ccgactctgc
    46501 tgtctcagaa tcagaagatg ttgtgtcttg tgtgcttgtt gactctgctt gaagagaggc
    46561 gctagtctgc aaataaatat cggaaatctt agcgacatga ttcgtataat tagccgataa
    46621 ttgattgatc aaagtatcta agtctacacc gtctttattt tctgctgcct gatacgctgc
    46681 acgtaatttt ctgatccctt cgtcggagat tgcttgaagc tcacgtgctt tttggttagc
    46741 aactgctgat aaaccagcta ttccctcttg atttccctga atttctgtct ggtattcttc
    46801 aatcaatgca ggcgtctttt cttcgatctt tttcgaataa tcatttacga ttgcttgaag
    46861 ttgctctggc aaaccatttg ctgttttttc ttcagaagtt tgtgtctctt ttgttttagt
    46921 tgatgactca cttgtttgtt tcgtctctgt tttctttgtc gttgtttccg ttatggtcgt
    46981 acttttcgct tctgacgaat tttttccatg aaaatataga cccgcaactc ctcctgctat
    47041 gatcaaaaac acggcaactg ctgccccaat catgatcatc cgcttacgag ctgcctgttt
    47101 ttgctgtctt cttcgggtca tccgaacatt ttctccccac tcattttcat catacatttt
    47161 tttcaccacc aatttttatg tataatagcg ttatcaaacg cttcatattc ttattgtagc
    47221 aaataattgt cgctgtgcac atttttatct ttgttattta taagaaaatt tcaccagttg
    47281 agatgattcc aagattgtcc gtcgatttct ccttgcagct gcattgttcg ttcgtctatc
    47341 tcagccatca gtttctttat ttcatataag agcgcacggt cttgatattc tgatttttcg
    47401 cttaattcat ctaattgttg gatcagccaa tcatttgtca taatgaagtt cctttcattt
    47461 cttgcagtgt aaaggtcaag tcttcgatct gctcttttgc ctcagtcagt gcttctacga
    47521 tatatgcttg tttttcagat tcttcttcct gcattttctg attttcctgt actctttgaa
    47581 taaaccagtc aatccgctga gtcagcgatg gtttgccttc caatgagaga tactgctgat
    47641 aagcaattag cttctcccaa cgtttttcct gggataaata cataaattgt gctaaaacga
    47701 atggcggaag ataggtcata tggaatttat ttgcgattcg ctgatacggc ctcaaaaatt
    47761 cggaaatcgt atattcttct tttcctccag cttggtattc tttttccgcc acacctgttg
    47821 tcaatacgat tcctagttct ttccctttta atacatgtgc tgctcgttcc aagacattat
    47881 cttgccaaat ttttaaatga gcaggagaac tataccagta aaacggaaac tggaaaatga
    47941 tccgatcatg ctgggccaag agctgtagtt cagtctctat atctattttt ccgtctggat
    48001 aacttgattc caaatgatgc caagtagctg gtaaaccagc agaagcttct ttgaaaaatt
    48061 gctgatttaa agattcttcg atcgttggat ggctaatgat gataagtgta cgcatccctt
    48121 ttcctccttt ttagtcttcg ctttacagtg tagcataacc gccacaagtt tcttttgccg
    48181 atgcttgtca ttttaaccag actaatcagg ttgtccaaga aactgcggtt tgtttttcca
    48241 actgtttttt caaggtatcc aaagcaatat tcttaaaatt ttcgtttttc atacgtttac
    48301 gatagttcgt agccgtttct ccggtactct ttttgaacat tcggctaaat acgactagac
    48361 tattgaaccc tacttgatag gcaatacttt ccacggattc atccgtcaga gaaagaagaa
    48421 acgcccctcg attgatccgt accccgatca aatattcctt gatactggta cccatatgat
    48481 ttttgaatac tcgcgacaaa tggctccgat tgaccgctaa atgctgggcg atttcctcaa
    48541 cagacaattt ttcagcataa tgtgcctcga tgtatttgac tgtttcaaca gctaaagagg
    48601 aatgaacctt ttgtacacca ccgctgacct ttccgccatc ttttaaaagg actgataaaa
    48661 aacggtaggt taatgctgtt aactgtaatt cattttccaa ggtttcttcc gtatctttca
    48721 tgcattctaa aataatgtcg atatattttt gtatgtcaga agagaccatc gtgtattggt
    48781 cttctctgaa aagcgactgc cgaatgattt cttctgcagc actcccacca aatgaaagcc
    48841 agcaatacat ccacggatct tctccatctg ataagtaaaa cgtcgagtct cctggacgaa
    48901 tcaagaacag atcacctttt ttcaattgga attgttgatt tttcacatga taagatcctt
    48961 tgccttccat cacgacatgc aggatgtaat cattgcgaat agcaggacca aaagaatgaa
    49021 acggcatcgt tttagagaaa ccacaaaaat caaaatacaa ttctttttct acttgttgat
    49081 gttccttttt taaaaaaatc gattgctcca cgtctatccc tccttactcc tttagtataa
    49141 tgcaacaaaa agttaaataa aagtcacaga aatatattaa aaaggaagcg atttcttgtt
    49201 aagtttaagt cattaaaaga tctttgttat tcgttgtaac cggtttactt agtattatat
    49261 attgaacagg agaggaatac agattatgaa aagcaaaatg atccaacgtg tgtcctacgc
    49321 tttaggcgct tttggacacg acacattcta tgttacatta agcacttatt ttatggtatt
    49381 cgtgacaagc cagatgttcg caggaacaga taaggcaacg aatgcaaaaa tgattgctac
    49441 agttacaagc ttagttgtag gtatccgctt agttgaaatt atttttgatc ctttgattgg
    49501 tggcatcatc gataatactc gtacaaaata cggtaaattc aaaccatggt tagttatcgg
    49561 cggcgttgtt agttccgtac tcttggtagc aatctttacc aatttctttg gcttagctac
    49621 tcataatacg actttattta tcatattatt tgcaatcgtt ttcatcttat tggatagttt
    49681 ttattcattt aaggatatcg ctttttggtc aatgattcca gctttgagta cggaaacaaa
    49741 ggaacgtgaa aaatttgcta cgtttgctcg tttcggctct tctctaggtg caaatggtac
    49801 tactctagtc gttgtcccta tcgttacttt ctttacctat cttgttactc ataaacatat
    49861 tgaaggcgct tcaggctggt tctggttcgg tgtgattgct gctgttattt caggcggtac
    49921 tgcgattatc actgccttgg gcacaaaaga agtcgatact gcgatccgtc agcaaaatga
    49981 aaaagctggg atcaaagatg tgttcaaagc agttgctcaa aatgatcagt tgatgtggct
    50041 tgcccttgct tatatcgctt atgcgattgc caatgcagca acaactgccg tattattcta
    50101 cttcttcaaa tatgtacttg gccaaccaac tgaattttgg atcgttggtc tagttgctgc
    50161 cttacttggt ttagtagctg ttccactatt cccaatgctt acacgcgtca tctctcgtcg
    50221 ctacgtttac cttggaggga tcgcaatgat gatttcttct tactttttct ttacgatcgc
    50281 aggttcaagt ttgccaatcg ttatcatcgg gctagtattg ttctacttcc ctcaacaatt
    50341 gattttctta tccgctttga tgacgatcac agactctgtt gaatatggtc aatggaaaaa
    50401 tggtatccgt aacgaagctg ttactctatc aattcgccct ttactagata aaatcgctgg
    50461 tgctttgtct aacgggatcg tagggtttgt cgctatcgct tctgggatga ctggtaatgc
    50521 tactgctgct gatatcactg ctcatggcgt aacgactttc aaagcttatg cattctatgc
    50581 tcctgctgct ttgatgattt tctccgctat tatttttgct tggaagatta cattgacaga
    50641 gaaaaaacat gcacaaatcg ttgagaaatt ggaaaacaaa ttggctccag ctgatacaat
    50701 ggaagattaa cttcacgatg cagtagttcc ttcccattaa gaaaggatct tgaaaatgaa
    50761 gattacagaa aaagattttg gccaaggtta tcacttgata accttggaga acaaaaacaa
    50821 gctagcactt tctatctctg atttaggtgc tagaatcgtc agtttaaaat caaatgatcg
    50881 agaattagtt ttgggatttg acaccgctga agaatatatc gaaaaagatc cgtatatcgg
    50941 tgcttctatc ggtcgcaccg cagggagaat cgaaaatggc cgcttttctc tcaatggaaa
    51001 aacttaccaa ttagccacag atccaaaaac tggacataat ctgcatggtg gtgctcctgg
    51061 atttgaatta aaaaaatggt cttatgtcat tttgaatggc gaaaatgaag cttctgtcat
    51121 ttttactact acaagtcccg acggagaaca tggttttcca ggtacaatgg atgtggaaat
    51181 ccgctatact ttgacaaaag ataatatctg gcgagtgacc tctcgcggca ccagtgacca
    51241 agatacttta ttcaatccga caaatcatgt ttacttcaac ttgacgggag atgcttctca
    51301 gtcaatcgat caacatgagc tttggctcaa tagcgaagct tatgctcctt tgcgaacaga
    51361 tagtattcct ataggggtaa aagaaaatgc tgcaggaagt gctttcgatt ttcaaatacc
    51421 taaaaaatta gcgtccgttt ttgctagtga tttggatcaa aaaaatcttg ttgacggaat
    51481 cgatcatcca tttttcttaa aagaaaccgg cttgggtaaa gaagccgctc gcttgactag
    51541 ccccgataaa agaatccaag tagatattgc tacagatgct tctagtgtgg ttattttcac
    51601 tgcaaatttc ggcacagaaa caccagaaat gcgcaaccga aaactggcac atcacggtgg
    51661 catcacattt gagactcaaa cagctccagg tgcagagcgc ttctcagcat ttggttcgat
    51721 tcatttaaaa gccggatcag tatttgaaac agtaacagaa ttcaaaatca aaacaagaaa
    51781 ggaataactc atcatggaaa tcaaaagaag cttaaacaca aaatttgccg aaacattcgg
    51841 acctaaaaaa actacacaat acttctcacc aggacgcatc aatttgattg gtgaacacac
    51901 agattacaat ggaggccatg tcttccccgc ttccatcact tatggaacgc aaggtgtagc
    51961 agcacctcga gacgataata aaatattagt ttattccaca aactttgacg atatcggtgt
    52021 cattagtttt actttagatg agctagaata tgataaaaaa gcaaactggg caaattacgt
    52081 aaaaggaatg gtccttaaat taaaagaagc tggacactca atcgatcatg ggtttgagtt
    52141 attagtggaa ggaacaatcc ctaatggtgc aggactttcc agtagcgctt cattagaatt
    52201 attagtaggt gttgtattag aagatctgtt tgatttaaaa acagatcgct tggaactagt
    52261 ccaaacaggt aaaaaagtag aaaatgaatt catcggtgtc aattcaggta tcatggatca
    52321 gtttgccatc ggcttcggtg agctagatca tgcaatctta ttagatacaa atacgctgaa
    52381 atacgaaatg gttccagtga aactagatgg ttatgcaatc gtcatcatga atacgaataa
    52441 gcggcgggaa ttggcagatt caaaatacaa tgaacgccgt agcgaatgcg aagaagcctt
    52501 ggcacgtttg caaaaaactt tgcctatctc ttctttagga gacttagatg aagaagaatt
    52561 tgaatcaact attgaccaaa tcggcgatga tacattgata cgcagagctc gccatgccgt
    52621 ttctgaaaat cagcggactt tgaaggcaaa agctgaatta gaagctggga acttagaagc
    52681 atttggtcaa ctgttaaatg attctcacca ctctcttcgc tatgattatg aagtaactgg
    52741 gatcgaatta gatacactag tagatgctgc tcaaaaacaa gaaggtgtct taggtgcacg
    52801 aatgacagga gctggatttg gtggctgtgc gattgctctt gtcaaagaag aaaatatacc
    52861 tgccttcaaa aacaatgtct atgatgaata catgaaagtt gtcggctatg ctccggaatt
    52921 ttatgttgca cacattggaa atgggacaac aaagatcgat ccagaagctg ttcatgtttg
    52981 ataaaaaaat caacgatctc agctaaaaac aaatagataa aaataacctc tcagcaattt
    53041 ccaaatgtga gccttttgct cgtatttgaa aattgctgtt ttttattgac acggaaagag
    53101 aacgtgtgtt ctaattgtgt tgaagaggtg attgttatgt gggcgaatca aaatccggta
    53161 tttgtttatg aaaaagaacc ttcaagagat attttatgta ttgactgcaa atctttttac
    53221 gcctccgtag agtgcgttga aaaggatctt gatcccctta caactaaatt agtagtcatg
    53281 tcctatccgt ccgattcgac agaaacacgg ggtagcggat taatcttggc ttccagtcct
    53341 gcagcaaaaa aagcttacgg catcaccaat atcagccgtg ccagagatct tcccttcccc
    53401 tatccaagtg atttatatat tgttgcccca aggatggcct attatatgga aaagaacaga
    53461 gaaatcaatt ctatctataa aaaatatgca gacgaagcga accatcatgt ctattccgtc
    53521 gatgaaagct tccttgatgt aacaaatagc atggcactct ttggtaaaac aaatgcgtac
    53581 gacatggcta aactgatcca taaagatgtg tttgagcaga ccggaatcta cacgactata
    53641 ggaattggcg ataacccgct acttgctaaa ctagccttag acaatggctc aaaagacgga
    53701 aagcaaatgt ttgctgaatg gcgttatgaa gatgtaccag aaaagttatg gaaaatctca
    53761 gatatcactg acttttgggg aatcggcaaa cgaacagcaa aacgtctgaa acaaatgggt
    53821 atacattctg tttatgattt agctcacgcg gattattatc acttgaaaag caagctaggg
    53881 gtcatcggta ctcagcttta tgcccatgct tggggcatcg atcgaagttt tttgggacaa
    53941 gaatatacac caaaatcaaa aagtatcgga aatagtcaaa tcctagatag agattataca
    54001 agacgagaag aaatcgagat cgttatcaaa gaaatggccg accaagtcgg cactcgcttg
    54061 agacgatctg cagcaaaaac acaagtagtc agcttatggg taggatactc tttaggctat
    54121 cttgatccaa atggcaagac cgggtttcac cagcaaatga aaatcgaagc gacaaatgct
    54181 agcagagatc ttgcagatgc tttattgacc attttccacc agcattacca ctaccaggat
    54241 attcggcgtg taggcgtgaa ctgttccgac ctcgtatacg cgacaggttt acagcttaac
    54301 ctttttgaag atcctgaaaa gcagataaat gaagcgaaag ttgattttgt tgtcgatacg
    54361 attagaaaaa aatacggctt tcaatcaatc atccatgcgc atagtctctt gcaaggagga
    54421 cgcgccatcg cacgtagttc tctcgttggc gggcatgcag gtggtatgag tggattagaa
    54481 ggtggaaaag atgagacgga cagcaaaaaa attcattgac tataacgagt atcgagatcg
    54541 gccatttgga ttaaaatggg ggactgcttt tgcgatggaa gaactagtca acggtatcaa
    54601 aaaaatgaag aagaagcacg caaacagcct cttcctaaac aacaaatgac attgcaagaa
    54661 atcgatagta tactgacaga agccttttta tctcgtaaag cagtaatcat tcagctcaat
    54721 ttaaaagatg aatttggaag actcttggat gaggtcgaag ggcacttcat tggtgaagcc
    54781 tatgaagatt actttatgat tgaccatcag ccgatattgt gggaagacgt acggtatatt
    54841 gagttaaagg aaacaaaaaa atggtttgtg acatgagagg ttgtgacaga agtgatcaac
    54901 ttcaaaaaat aagaaaggat ttttcgaaaa ttgcttctta aatttttgtg gaattttggc
    54961 ttatttttcg aagaagcaac ttctggaaca acgtatatta gatatcagag gttgtgaaaa
    55021 agtcgcttag ttttgagtaa caagccaagg cgctgatttc acaacctctg tctatctatc
    55081 catctattcg gtaagattca tgcatgacgt cttctttatt tagtatgtag agcccttttt
    55141 cttctgacca ttcaacataa atcagctgtt ttacatctgc atagccttct ttcttcaagt
    55201 tctccataag ccatgcttca gtcaattgat tggactgaag cactttatgc tgaatttgcc
    55261 cttcatctac aagcaggatc gataaagctt tgtcttcatc tgattttttc aatacactca
    55321 tttgaccggc attctctagg ataacatgtt tggcattttc aagaaaaaag cattcttgct
    55381 gtcttaagat tgctcgcaac tgctccatct cgatatggtt atttgtcaac tctgtcatgt
    55441 tgatgacgcc gtctttgatt agaacagatg gctcgccttt gacccaacga ttgacctttt
    55501 catttttttg gacgatacgt tcgataccat aaatcattac tgcccaaagc gcaatggcaa
    55561 ataggacatg ccctacatgg acattgtcat cataggttga ttcttctaag atcccaccta
    55621 aaacaagcgt atagactaaa tcaaacggtg tgatttctga catggatttc ttcccaagca
    55681 aacgtaccac taataataaa ccgatcaacc cgaacaccag tttcattgta attgaaaaaa
    55741 tcattttttc tccccctaac tatctgttaa ttaaattata tatgctttct cacgaacacg
    55801 catggaatat acaaggaaga aatgtatcct tttactaaaa aaaggactgt gacactgtag
    55861 tgacccccaa aagttagact tttttggagt ggagaaatcc actccttttt tttatgctct
    55921 aaaatgatat ttttacacaa ccgttacggt tgttttacga aattggacg
//
LOCUS       DANJMI010000017        54630 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000017 DANJMI010000000
VERSION     DANJMI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.017"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(592..858)
                     /locus_tag="PWU81_001193"
                     /pseudo
     CDS             complement(592..858)
                     /locus_tag="PWU81_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1696..2424
                     /locus_tag="PWU81_001194"
     CDS             1696..2424
                     /locus_tag="PWU81_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1109909.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
     gene            2442..3635
                     /locus_tag="PWU81_001195"
     CDS             2442..3635
                     /locus_tag="PWU81_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL1109910.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            3628..4623
                     /gene="lacD"
                     /locus_tag="PWU81_001196"
     CDS             3628..4623
                     /gene="lacD"
                     /locus_tag="PWU81_001196"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL1109911.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            4620..5561
                     /locus_tag="PWU81_001197"
     CDS             4620..5561
                     /locus_tag="PWU81_001197"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HDL1109912.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            5607..7373
                     /locus_tag="PWU81_001198"
     CDS             5607..7373
                     /locus_tag="PWU81_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDL1109913.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFTTGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            7390..7869
                     /locus_tag="PWU81_001199"
     CDS             7390..7869
                     /locus_tag="PWU81_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HDL1109914.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            7882..8772
                     /locus_tag="PWU81_001200"
     CDS             7882..8772
                     /locus_tag="PWU81_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1109915.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            8759..9568
                     /locus_tag="PWU81_001201"
     CDS             8759..9568
                     /locus_tag="PWU81_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL1109916.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            9584..9988
                     /locus_tag="PWU81_001202"
     CDS             9584..9988
                     /locus_tag="PWU81_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1109917.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(10169..11107)
                     /gene="yidC"
                     /locus_tag="PWU81_001203"
     CDS             complement(10169..11107)
                     /gene="yidC"
                     /locus_tag="PWU81_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL1109918.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(11441..12523)
                     /locus_tag="PWU81_001204"
     CDS             complement(11441..12523)
                     /locus_tag="PWU81_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL1109919.1"
                     /translation="MTKNLIVRGAPQEYEIKENAWDSLLEHLYKRHIDNVLILHGKTS
                     WKAVKDVFPDLSITKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKL
                     IAYKTNLPIIILPTLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHS
                     PREMMIAGIGDTLAKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAM
                     DQQKINQAFIDVVETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKV
                     AYGILVQLMIENKLAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEM
                     IHFMKETITPALVVQKMKELEQVTQG"
     gene            12665..14116
                     /locus_tag="PWU81_001205"
     CDS             12665..14116
                     /locus_tag="PWU81_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDL1109920.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
     gene            14109..14831
                     /locus_tag="PWU81_001206"
     CDS             14109..14831
                     /locus_tag="PWU81_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL1109921.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            14851..16002
                     /locus_tag="PWU81_001207"
     CDS             14851..16002
                     /locus_tag="PWU81_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL1109922.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(16350..16625)
                     /locus_tag="PWU81_001208"
     CDS             complement(16350..16625)
                     /locus_tag="PWU81_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL1109923.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            16733..17497
                     /locus_tag="PWU81_001209"
     CDS             16733..17497
                     /locus_tag="PWU81_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDL1109924.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            17621..18124
                     /locus_tag="PWU81_001210"
     CDS             17621..18124
                     /locus_tag="PWU81_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL1109925.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            18389..19036
                     /locus_tag="PWU81_001211"
     CDS             18389..19036
                     /locus_tag="PWU81_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HDL1109926.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     gene            19197..20465
                     /locus_tag="PWU81_001212"
     CDS             19197..20465
                     /locus_tag="PWU81_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HDL1109927.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     gene            complement(20642..21844)
                     /locus_tag="PWU81_001213"
     CDS             complement(20642..21844)
                     /locus_tag="PWU81_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL1109928.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
     gene            22371..23096
                     /locus_tag="PWU81_001214"
     CDS             22371..23096
                     /locus_tag="PWU81_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HDL1109929.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            23086..23760
                     /locus_tag="PWU81_001215"
     CDS             23086..23760
                     /locus_tag="PWU81_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HDL1109930.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(24048..24332)
                     /locus_tag="PWU81_001216"
     CDS             complement(24048..24332)
                     /locus_tag="PWU81_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HDL1109931.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(24626..25933)
                     /locus_tag="PWU81_001217"
     CDS             complement(24626..25933)
                     /locus_tag="PWU81_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL1109932.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
     gene            26422..27528
                     /locus_tag="PWU81_001218"
     CDS             26422..27528
                     /locus_tag="PWU81_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109933.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
     gene            28119..28319
                     /locus_tag="PWU81_001219"
     CDS             28119..28319
                     /locus_tag="PWU81_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL1109934.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            28466..31255
                     /locus_tag="PWU81_001220"
     CDS             28466..31255
                     /locus_tag="PWU81_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL1109935.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            31302..31829
                     /locus_tag="PWU81_001221"
     CDS             31302..31829
                     /locus_tag="PWU81_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HDL1109936.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            31850..33040
                     /locus_tag="PWU81_001222"
     CDS             31850..33040
                     /locus_tag="PWU81_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL1109937.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            33080..34378
                     /gene="asnS"
                     /locus_tag="PWU81_001223"
     CDS             33080..34378
                     /gene="asnS"
                     /locus_tag="PWU81_001223"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL1109938.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            35312..35986
                     /locus_tag="PWU81_001224"
     CDS             35312..35986
                     /locus_tag="PWU81_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL1109939.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            35983..36324
                     /locus_tag="PWU81_001225"
     CDS             35983..36324
                     /locus_tag="PWU81_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109940.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            36354..36713
                     /locus_tag="PWU81_001226"
     CDS             36354..36713
                     /locus_tag="PWU81_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDL1109941.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            37237..39318
                     /gene="cadA"
                     /locus_tag="PWU81_001227"
     CDS             37237..39318
                     /gene="cadA"
                     /locus_tag="PWU81_001227"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL1109942.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            39629..41095
                     /locus_tag="PWU81_001228"
     CDS             39629..41095
                     /locus_tag="PWU81_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1109943.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            41405..41878
                     /locus_tag="PWU81_001229"
     CDS             41405..41878
                     /locus_tag="PWU81_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HDL1109944.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFIVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            41952..42392
                     /locus_tag="PWU81_001230"
     CDS             41952..42392
                     /locus_tag="PWU81_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HDL1109945.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            42405..42614
                     /gene="copZ"
                     /locus_tag="PWU81_001231"
     CDS             42405..42614
                     /gene="copZ"
                     /locus_tag="PWU81_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDL1109946.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            42614..44800
                     /locus_tag="PWU81_001232"
     CDS             42614..44800
                     /locus_tag="PWU81_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL1109947.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            44820..46991
                     /gene="copB"
                     /locus_tag="PWU81_001233"
     CDS             44820..46991
                     /gene="copB"
                     /locus_tag="PWU81_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HDL1109948.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(47103..49274)
                     /locus_tag="PWU81_001234"
                     /pseudo
     CDS             complement(47103..49274)
                     /locus_tag="PWU81_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(49264..50205)
                     /locus_tag="PWU81_001235"
     CDS             complement(49264..50205)
                     /locus_tag="PWU81_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HDL1109949.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            complement(50350..51198)
                     /locus_tag="PWU81_001236"
     CDS             complement(50350..51198)
                     /locus_tag="PWU81_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDL1109950.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            51552..52841
                     /locus_tag="PWU81_001237"
     CDS             51552..52841
                     /locus_tag="PWU81_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1109951.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            52915..53484
                     /locus_tag="PWU81_001238"
     CDS             52915..53484
                     /locus_tag="PWU81_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL1109952.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(53660..53926)
                     /locus_tag="PWU81_001239"
     CDS             complement(53660..53926)
                     /locus_tag="PWU81_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1109953.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            53921..54613
                     /locus_tag="PWU81_001240"
     CDS             53921..54613
                     /locus_tag="PWU81_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL1109954.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
BASE COUNT        17847 a         9490 c        11481 g        15812 t
ORIGIN      
        1 actatttatc attactgtac tagctgtttt atcgatgaca gattttattt aacttatctc
       61 aaaaagtagg gaaccatatc atttttcagt aacatgcgta ttatttgtat aaataataca
      121 aaatgaaaaa ttatgattat tctattgtct ttttagaaag cgttttctgt gagcttttaa
      181 tctaaaagga gggatataaa ataaagaaaa cctattttat gctatttttt tgttgtttct
      241 gtgtaggaat cctgtggtat ataggtggta atgaaaagaa gattcatgga gttgtgacaa
      301 atatttctgc ggatgagttc gtatttgaag atgaaaaaca gaacgaatat agtattcaaa
      361 aaagaagctc tttgtcaaat gatgttggta aagagaaatc ttagaataat ttggacagaa
      421 gaggctggga cagaagtgtt taactccgag aaataagaag aaattcacga aaattgcttt
      481 tcaaattttt gtgaatttcg gcttatttcc gaaggagttg cttctgctcc cgccgtttac
      541 tcgagagtct gtagcaaaac tgatttttag atttatccca cattcttctg tttatttggt
      601 taaaaatgtt ttgccatgac gaatcatgat ccgtagtttg aaattgtaaa agctgcgaaa
      661 gccatacgta ttgcgtttta atactttgat gtggttgttt aaacattcca atggtctgtt
      721 tgaatagctg cagttaagcg cattttgaat ctgtggcaga aaatttttaa acgttcgtaa
      781 agtggttgta taggtttcag gcagatcgct aatgtctcta gttaaacagt cctcaaatag
      841 tgacgaatct cttttctttc actcaatgat tcaagaaggt tcttttaata tattggaaga
      901 tgtggatatt cttctggtgt gtactgaaga gtccctttgt aaatcaagca aatttgtcct
      961 ttgtattttt ctttagttaa acaattttcc tcgaaatgga tattgatatt taggtctaga
     1021 atatttaggg tatctttgat aagcagagta tgagacatct tgaatcctcc tatgaatttg
     1081 ttgggcaaat taattctaaa ggatttcagg gtgtcttttc tatatttttt tatacaaaaa
     1141 agatgttgat aaagttcaca aacatcgaaa agtatagagc ccgaatttta cgaatatttt
     1201 ttgtaatata aaagtataga tactaaattc ttattcgtta aacttacgcc ataaggaaac
     1261 tagaaaaaga cagaattatc ataaaaaatt tttgttattt ttaaaaatgt acaacaaaat
     1321 ttcgactatc agaccggcat gagagaaaag ttatctaaaa aataaaaaat cctactttat
     1381 ttagagaaag ccatttttat ttacatagta gtctattagg cccatttgct tgtcgatagt
     1441 agaaatagcc acagatgtat aaagaatatt ttttctttac ttctctagac catttagctg
     1501 aatctttgcc aagattcagc ttttttttgt tttttatttg tcaactggta tttttgaggt
     1561 atcttttgat accagttcta aattaccttg aaaccttgtt ataatagctt ttttacgcca
     1621 aaaacccttt gacaaatgag gaatatgtgg gtattcttgt gttgtggtta taaccagtaa
     1681 agaagaggta tttttatgga gagctcaagt aaaggaaaat cattatatta tcaactagtt
     1741 gacgtattga aagaacgtat cgaaacacag atgacacctc atgacaaatt gttatctgaa
     1801 agagaacttt gtcagcatta tggagtgagt cggacaacgg ttcgccttgc tttgcaagaa
     1861 ttagagacgc aagggtatat ttatcgccga cacggaaaag gaacatatgt gtctgatcta
     1921 tcagataagt cgtctgatct ggcgggagcg tatagtttta cagaagagat gagggaattg
     1981 ggacgggtgc ctaatacagt catcttggaa ttcaagcaaa tggaagcaaa caaaaaggtt
     2041 gccaagaatc taaacatcaa tttaggcgaa acagttttca aattgaaaag acttcgttta
     2101 gcggatgacg aaccattgat ggtggaacgt tcttatttac ctgcaaaact atttattggt
     2161 cttgatctgc cattacttga aagtaagcca ttgtatgatg tgttttctca ggattatgaa
     2221 cagcaagtcc aattagcaga tgaagaattt tatgcaagta tcgttcaaaa gaaagatgct
     2281 gatccgcttc gtatcgctga tggtgccccg gtattatatc taagccgaac gacctataat
     2341 gtgaaaaatg aaattattga atatacggat agtgtagcga gagcagacca gtttcattat
     2401 aagatccgcc acgtccgaaa atgattgaga gggaaaacaa catgtttgaa ctatcgaagg
     2461 ataaattaaa agagttgggt gcggaaataa ctacgcgaga aatcaaacag caaccgcaat
     2521 tatggaaaga aacagctgag atattagcac agaatcaaga aaaattagca ttgttttttg
     2581 accagataaa aaaagcagca aatggcaaaa gagttcgagt gatttttact ggtgcaggca
     2641 catcacaata tgtcggggat acagcagtac cttatttaaa tgaatacggt gataacgatc
     2701 actttatttt tcatagcttt ggtacgacag atatcgtgtc ttcaccaaaa gaatttctat
     2761 ttccggatga accgacatta ctggtgtcat ttgcacgtag tggaaatagt ccagaaagtg
     2821 tggcaacagt caagattgcc aatgaacaaa tcaagacatt gtttcatttg acgattactt
     2881 gtgctaaaga aggtcagtta gcaaagaatg cagcgacaga tgaacgaaat tatcttttac
     2941 tgatgccaga aaaatcaaat gatgcaggat ttgccatgac aggaagtttt acgtgcatgt
     3001 tgttgagtgc gttatgggta ttcgaccaaa ctgccgttga gaaaaaagaa agttatcttc
     3061 aacaattgat cagaactgga gaagatctaa tttcacgaga tcaagaaatc caaaaattat
     3121 tgcctaagtc atttgaacgt atcgcctatc ttggttcagg aagtttggct ggtgcaacga
     3181 gagaagcaca attgaaaata ttggaattga cagccggtaa agtggcaacg atctttgatt
     3241 cttctatggg cttccgccat ggaccaaaat cgtttttaaa tagcgaaaca cttgttattg
     3301 gttttgttaa taaccaaccg tatacaagaa attatgactt agatattcta gaagaaataa
     3361 aagatgacca aattaccacg agtgtcatcg cgatcgccca accaggaaac atcgatttca
     3421 caggtacaag tcttttgtta gaagcttcga tcagcctacc tgatgcgtat cttgccttac
     3481 ctatgatcat gacagcccaa atcattgcgc tcgatacgtc gataaaagta gcgaataaac
     3541 cagatacacc atcgcctaca ggtacagtca atcgagtggt caaaggtgtc attatccatg
     3601 aatatgtgaa caaaggggaa acgaaggatg aatgaagaaa aaattgcagc tatgaagagt
     3661 ttatcaacaa aagaaggaat catcagtgca ttggcaattg atcaacgcgg tgctttgaaa
     3721 aagatgatca aagcgttagg aaaagaacca acggcgcaac agatcactga attcaaagaa
     3781 ttagtatcag cagaattaac gccttatgct tctgctattt tactagatcc agagtatgga
     3841 ttgccagctg ctaaagccag acatcaggaa gcaggactac ttttagccta tgaaaaaacg
     3901 ggttatgatg cgacaactcc aggacgtctg cctgatttat tagcggattg gtctacgctt
     3961 agattaaagg aacaaacagc cgatgcgatc aagtttttgc tttattatga tgtagatgaa
     4021 gacccagaga tcaatcattt gaaacatgta tttatcgaac gtttaggcag tgaatgtgca
     4081 ggtgaaaagc gtccgtttta tctagaattg gtttcgtatg atgccaaaac caatgacagc
     4141 acttcattag aatatgcgaa gatcaaaccg cataaagtga tcgagatgat gaaagaattt
     4201 tctgatccta aatataaagt ggatgtattg aaagtagaag tgccagtcaa tatgaactat
     4261 gtagaaggtt attctgatgg tgaaattgcg tatagtaaaa aagaagcagc ggcattcttc
     4321 aaggaacaaa gtgatgcaac accgttacca tttatcttct tgagtgcagg ggtttcgact
     4381 gcattgttcc aacaaacatt gatctttgct catgaatctg gttcaacatt caacggtgtg
     4441 ttatgcggac gtgcgacttg gaaagatgga gtcaaagcat atgttgaaaa tggtagagaa
     4501 ggtgcgattg agtggttgag aacacaaggg cgacaaaata tcgaatcgtt gaataaagtg
     4561 ataaatgaaa acgccacccc ttggtttgac aaagtaaaag aagattcggt gacagcaaaa
     4621 tgatcacgac agttaccatg aatccttcaa ttgacatcgc ttacacgttg gatgaattac
     4681 ggatcgacca agtcaatcgg gtcaaaacgg tcaacaaaac ggctggagga aaaggcttga
     4741 atgttgctcg tgtccttcat ttattagcag atgatgtttt agctactgga ttagtcggcg
     4801 ggcagcatgg agcatatatc aaagaacaac tcgatcaatc agggataacg catcaatttt
     4861 tacctatcaa aggggaaacg agagattcga ttgctatttt acatggagtg aatcaaacag
     4921 aactattgga aagcggtcct gttgtgacgg aagaagagca acataaattt accgagcatt
     4981 ttgcacgact aatggaaaca actgatatct ttacgatttc gggtagtctg cctcaaggat
     5041 tttctaaaag ttattatgca gagttacttg ccctaacgaa taaaaaagga acacgagtat
     5101 tgttagatac ttctggtcaa accttgcttg aagtcttgga aggcaaggaa aaaccatttt
     5161 tgatcaaacc taatttggaa gaattaggag agttggtcca ccaaacaatt tcttcggcta
     5221 cttcgttaga tgaaatccaa tcattattgg atcatccgct ttttgaagga atcaattgga
     5281 tcgttctttc gctaggcgca aaaggagcaa tcgctaaagt agggagacac ttctatcagg
     5341 ttacgattcc taaaatcgcg gtggtgaatc ctgtggggtc aggagattcg actattgcag
     5401 gcttagcaca tgctctatca aagagctata cgattgaaga aacacttgcg tttgcgatgg
     5461 cctgtgggat ggcgaatgca caacaagaaa aaacaggatg gatcgatcct gtgaaagtcg
     5521 attattttag gggggaaatc aacgtcagaa agattcgttg attataaaat tagaaagcgg
     5581 tttctaaaaa cgaggaggaa atttgcttga ctagatttga aataaaagaa gagtttttgt
     5641 tagacggtca acctttcaaa attttatctg gtgccatcca ttatttccga gtgcatccga
     5701 cgaattggga gcggtcattg cacaatttga aagcgcttgg atttaatact gtggaaacct
     5761 atgtgccttg gaatctccat gaaaaagaag aaggaaccta tgatttttcg aaaatattgg
     5821 atctttccca ttttctagag ttagcgcaat cccttggatt atacgcaatc gtacgtccag
     5881 ccccttatat ttgtgcagaa tgggaatttg gtggcttgcc tgcttggcta ttgacaaagg
     5941 agtgccgtat tcgttcagcg gatcctcgct tcatgaagta tgtcaaacgg tattaccaaa
     6001 cgctactacc taaaatcgtt cctcatctag tcactcatgg tggcaacgtc ttaatggtgc
     6061 aggtagaaaa tgagtacggt tcgtatggtg aagagaaagc atacctgcgc caagtaaaaa
     6121 aatacttgga ggaaggtgga atcgatgtac cgctattcac ttctgatgga ccttggcaag
     6181 ccacattaag agcaggcatt ttgattgacg atgatgtttt tacgacaggg aattttggct
     6241 caaaagcaaa agaaaatttt gcagatatgg cccagttttt tgaagcacat ggtaaaaaat
     6301 ggccgttgat gtgtatggaa ttttgggatg gctggtttaa tcgttggaaa gagccgatca
     6361 ttaaaagaga tccggaagaa ttagcggatg cagtcaaaga agcattacag atcggcagta
     6421 tcaatctcta tatgttccat ggcggaacca attttgggtt tatgaatggc tgttcagcca
     6481 gaggcgcgat tgatttgccg caagtcactt cctatgatta cgatgcacca ttggatgagc
     6541 agggaaatcc aacggaaaaa tattttgctc tgaaacgaat gatggcagaa aattttccag
     6601 aaatgcagca aatggaacca tggataaaag aaacaatcgt caaaaaagga atcaacttga
     6661 aagaaaaagt cagctttttc gcaacactgg ataccatcag caagccgatc cactcgacct
     6721 atcctcaaac aatggaagaa ttgggtcaga atactgggta cctattgtat cgaacaacga
     6781 tcaaaaaaga tgcggtcaat gaaaaattac ggatcatcga cggacgtgat cgggtccatg
     6841 tttttctcga tgaagaaaag caagcgattc aataccaaac agagatcggt gagaccattc
     6901 cattgacact tgagaaagaa gaccatcaaa ttgatctatt atttgaaaat atgggacggg
     6961 tcaactatgg acacaagctg ctagctgata cgcaaagaaa aggaatacgt acaggagtga
     7021 tgagcgatct tcattttatc acagaatgga cacagtattg tttaccttta gagactacag
     7081 aacacatcga tttttcaaaa aaatgggaag caggtcaacc agcattttac cgctttgaag
     7141 cggaactgtc agaaatcggt gatacttatt tggaccttag tgagtttggg aaagggatcg
     7201 tttgggtgaa tggcacgaat attggtcgtt tttgggaagt aggaccaatt ctttcactgt
     7261 ttgttcccga gggtttattt agaaaaggac aaaatgaaat cgttatcttt gaaacagaag
     7321 gtagattcag tgaagttata gattttgtca aagaaccaat agaaaaaagt tagaaaagga
     7381 gaatggatga tggcaattat tggagtaaga attgatggac gtttgataca tggacaagtg
     7441 gcaaatcttt ggacgacaaa gttgaatatt ggtcggatca tggtcgtaga cgatgaggta
     7501 gcaacaaatg cgatcgaaaa aagtgggtta aagttggcaa cgccagctgg agtcaaatta
     7561 agtattttac ctattgaaaa agcagccgca aatattttag caggtaaata tgattctcaa
     7621 cgagtgctga tcgtagcgaa aaaaccagat cgtatcttag cgctagtaga aaaaggtgta
     7681 ccgatcaaag aattaaatgt cggcaatatg tcacaaacag atgaaacacg ttccatcaca
     7741 aaatcaatca atgtcgtaga tgcggatgta gaggtcttca agaaattaga tcagcttggc
     7801 gttcatttga tcgctcaaat ggtgccaaat gacaaagcag aagactttat gagtctttta
     7861 atcaaatagg aggaatgatt catggatatt caatggtggc agatattatt attaacatta
     7921 tatgcagggt atcaaatttt agatgaatta cagatttatt cttcaatgag cgccccagtt
     7981 tttgcaggat tatatgctgg gttagtaatg ggtgatttaa aagcaggttt aatcattggt
     8041 ggaagtatgc agttgactgt tttaggggta gggacatttg gtggtgcatc aaaaatcgat
     8101 gccaactctg gaacgatctt agcaacagct ttctccattt ctctagggat gaacccagaa
     8161 caagcgattg cggccatcgc ggttcctgta gcaagtttga tgatccaatt agatattttg
     8221 gcacgttttg cgaatacgta ttttgcacat cggatcgatc aacatgtgga aacaatgaat
     8281 tacaaagcaa ttgaacggaa cttcttgatg ggggcattac cttgggcact ttccagaatg
     8341 gttcctgtat tcttggccct agcattcggt ggtggcttag tccaaaatgt tgtggctgtt
     8401 ttaaacggcg acctaaaatg gctaggagat ggcttatctg ttgcaggggc agtcttacca
     8461 gcagttgggt ttgcaatttt actacgctac ctaccagtaa agaaacacat ttcttatctg
     8521 attttaggat ttacgatcac agcgttatta gcaacagtgt tctcgaatgt ccaattgtta
     8581 ggaacaagtg tggctagcgt ggtcaaagat ttcccaggaa cattcaattc attaccgatg
     8641 cttgctgtgg cattgattgg tctagcactg gcaacgatca gttataagaa tgatcaaaag
     8701 aatgaccaac atccagtagt aagtcagcaa tcacaatctg atgaggggga aatcgaagat
     8761 gacgaaatct aattataaat tgaccaaaga agattttaag cagatcaatc gtagaagttt
     8821 gtttactttc caattaggtt ggaactatga aagaatgcaa ggttcaggtt atctgtatac
     8881 tatcttgcca caacttagaa aaatctatgg tgatgataca ccagaattaa aagaaatgat
     8941 gaaaacacat acgcaatttt ttaatacgtc taatttcttt aacacgatca ttactgggat
     9001 cgatcttgct atcgaagaaa aagaaggagt ctcttcaaag gaaactgttt ctgggatgaa
     9061 agcaggattg atgggttcct tcgctgcaat tggtgattct gtatttgcgg cattgatccc
     9121 gaccatattt ggagctttag ccgcaaatat ggcaacagat ggtaatccag taggtatcct
     9181 tatctggatc cttgctcagg tcgctgtcat ggtctttcgt tggaaacaac tagaatttgc
     9241 ctacaaagaa gggatttctc tagttaccac gatgcaatca cgcttgactg cattgacaga
     9301 tgcggctact ttactaggag ttttcatggt tggagcgtta gtagcaacca tgatcaatgt
     9361 taaattctca tgggcaccaa cgattgggga tgtaacgttg aatatgcaaa actatatcga
     9421 tatgatctta cctaaacttt taccagctgg aatcgttggt ggcgtttatt ggatgttagg
     9481 taagaaaaat atgacaccta ctcgagcaat attcatcgta ttgatcgtat gtgtagcatt
     9541 atctgcatta ggtgtgattt cgaaataagg gagaattgga aagatgagta gacaactagt
     9601 cttgattagt catggtaaat tttgtgaaga actaaaaaac agtacagaaa tgatcatggg
     9661 accacaagaa aatatctata cgatcccttt attgcctgaa gatggcgcgg acacgtataa
     9721 agaaagattc ctctccgtta cagcaccatt gaaagattac gtagtgcttt gcgatctgtt
     9781 gggtgggaca cctgcaaata ccgtttccaa gctaatcatg gaaggtgaaa cgatcgaact
     9841 atatgctggc atgaatttgc cgatggtcat cgaattcatc aattcgcaaa tgattggagc
     9901 agaaccagat tttattggtt cgggtcgtga gagtatcgtt tcagtgaacc aagtgattgc
     9961 tggcatggga gacgatgaag aagaataatc aagcacaact tcttgactgg tgatacaact
    10021 ggatataaat acggatgtat aaagaggccg tgtataaaaa agccaacgta ccgcgaaata
    10081 tagttttcta tatttcacag tacgttggct tttatcgtca taaaatcaga aatgatctgg
    10141 gtcgatcgaa gaagctagtt acaaataact atttacgctt ttgctgttta ccagcattcc
    10201 gaccattctt cgcattgttt tttggtgctt gtacttgttt ttttgtttgc ttcgcaggtt
    10261 cagcaggtgt gacatcttta cgaggcgtca ccacttgttt tggcggattt tgttttaatt
    10321 cttccgcaac ttgcgctttt aatcttggtt tcatgatgac attcgtaatg aatgtttgta
    10381 gacaactaaa gatcccaccg actacccagt aaagagtgac accagctggg gcactgatcg
    10441 acatgaagac gatcatagcg ggtgatacga tcaacatcgt acgcatcgtt ttcttttgtt
    10501 cttcagggat accgatagtt gagatatagc cttgaagtaa ataagcaata ccggcaatcg
    10561 ctactaaaac taagctaggg ttacctaggt cgatcccgta aaaggttgat tcacggatgc
    10621 cttctgtata gcgagcagtg aaatatagcg cagaaaagat cggcatttgg atcagaagcg
    10681 gtaaacaacc gataccacca gtcatactta agccattttc acgataaact tgttgcattt
    10741 cagcttgtgc agccatttgt tcttctcgag ttgtcgcagc tttcaatttt tgttgcgtag
    10801 cttcaacttg tggtttcaat gcttgcatct tttcagattg gatcattgtt tttttcgatt
    10861 gactaatacc taatggcaaa atgatgatac gaacgatgac tgtcatcaag ataacagccc
    10921 agccgtagga ccaattaaag ttttccacta aatatgtaat cgcttgtccc attgggtgaa
    10981 ccaatatacg gtaaatgatt cctgaagtgt cagggttacc atttgaatcc atgcggacac
    11041 aaccggacaa gaaaagcatg atcgtgacaa gcccagagcc caataaccaa tttttccatt
    11101 tattcataaa atacttcctc ttttataatt tttacacaag cttaactata ctaaaaaagc
    11161 gctcataatt caatcaactt catagaaatt tcatagaaat aaccgattgg aggatcaaaa
    11221 aagacgctta atgttaaaaa gaagaatcca gctaaaaata cttcagtata aagcatactt
    11281 agcacctccg ttctctaaaa taagatgagc aacggatatt tccttgaaat cataatttta
    11341 gcgcaaaaaa aagaagtgga atgtcaattt tttccacttc ttttttttta ccattgagcc
    11401 gatccataaa aaagctgact gccatggtat aaggggctaa ctatccctgt gtcacttgtt
    11461 ctaactcttt cattttctgg acaaccagtg ctggtgtgat cgtttctttc atgaagtgga
    11521 tcatttcatc aggtaaacat gcacgttcag ctacggcttg atagtcactt tctgttaaat
    11581 gtaaacccat atcagctaaa ctcgttggta agttcaagtg atgataaaac ggcagtaggt
    11641 gttcgatttc tgctaattta ttttcgatca tcagctggac aaggataccg tatgcaactt
    11701 tgtttccatg aagttgctga tggctatcag ggagtacggt catcgcatcg tgaatggaat
    11761 gagctccagc tgttcggccg tagtcatcgc caaacccacc aaccatacca gccaataaaa
    11821 tgtttgtttc cacgacatcg ataaaagctt gattgatttt ttgctggtcc atcgcttcca
    11881 ttgcctctgc acttttctct aaaaggattt cgcggcatcg taacgctgca aattccgcta
    11941 cttggacttc gataggcttg atagttaact gagagatgat tgcgtgagat tcataccatt
    12001 ttgctaaggt gtcaccaata ccggcaatca tcatctcgcg aggggagtgc aaaatgatct
    12061 ttggatcgat caacactaaa tcatttgctt tagggaacat atcatagcga agcatcactc
    12121 cttcttcatt atagaccaca ctaagtggag tataagctgc gcacgtcgca gctaaggtag
    12181 gtaagatgat gattggtaag tttgtcttat aggcaatcaa ttttcctaga tctgctactt
    12241 tcccgccgcc gactgcaata ataccattga aatccttctt tttgcataaa gaaacaaaac
    12301 tgtcagcaag tggatcggta cattctccac cataatattc aaaaattttt gttattgata
    12361 aatctggaaa aacatctttt actgctttcc aagatgtttt tccatggaga atcaaaacgt
    12421 tgtcaatatg ccttttgtaa aggtgttcaa ggagtgaatc ccatgcgttt tcttttattt
    12481 catactcttg aggggcaccg cgaacaatta aattttttgt catataaaaa acttcttcca
    12541 ttattttttt aaattaatct ttttttatta taatagttat gctagtatat tagcaagtta
    12601 tttatgaata tttattcttt tatagctttt ctgattacat tattattcaa aggtggccaa
    12661 atggatgaaa aaaggaataa acatattatt agtggcgctg atgtttctac tggttttttt
    12721 accagtaaaa gtacaaggtg cagaaaaaga tgaaatttac gagcggataa aacaaagtgg
    12781 agaattagtc gttggcctat ctgcagacta tgcgccctat gaattccatg cgaatgtgga
    12841 tggtaaggat aaaatcgttg gttttgatgt atccattgcc gaaaagatcg caaaggatat
    12901 gggtgtgaaa ctaaaagtag aagaacttgg ttttgatgca ttattaggtg cgttgaaaac
    12961 tggcaagatc gatttagtta tatctggtat ggcggtgact gaagaacgtt tacaagaagt
    13021 tgacttttct gacccatatt tcgtggtaca gcaaaaagta ctcgttcgta aagaggacaa
    13081 aaataaattt aagacgacag ccgacttttc tggcttagcc gttggggcgc aaaagcaaac
    13141 cacacaagag gagttagtga aaaatgaact gactggtgca gagttgacct ccctgcaaaa
    13201 agttcctgat ctgatcaata atttgctgaa tgaaaaagtt gaaggtgttg tgctggaaca
    13261 accagttgca gaagcatatg cccaacagaa taagaaagta gctttgactc cagtaaaatt
    13321 tgcgcaaggc gaaaaaacca cagctattgc gatgtcaaaa aatacaccag aactgaaaaa
    13381 tcatgtcaat gcgtcaatca aaacgatcaa tgacgacggc ttgctgaaag attatcaaaa
    13441 acaagcgaat caattgatgt tccaagatga tcagtccttt cttcaaaaat acggtccgta
    13501 ttatttgatt ggaacaaaat acactatttt attagctttt gtgggtgtat tgtttggaat
    13561 ggtctttggt agtttgcttg cactgatgaa attgggtaag aataaatttt taaaggcatt
    13621 agccattatt tatatcgaat acgtacgggg aacacctctc ttagttcaaa tctttatcgt
    13681 ttacttcgga acaggagtac tgggattgga tctatcgaaa ctggtagcag gttgtattgc
    13741 tgtctcgttg aatagtgctg cttatgtagc cgaaattgtc cgtgccggga tcaatgcggt
    13801 caacaaagga cagatggaag ctgcacgttc tttaggaatg gaccagaaag aagccatgcg
    13861 tttcatcatc ttcccacaag caatcaaaaa tattcttcca gctctaggaa atgagtttgt
    13921 cacgatcatc aaagagtcct cagttgtttc ggtcattggg gtatcggaat tgatctttca
    13981 aacgggcgtg gtacaaggtg ctagcttcaa accattccta ccatatgtgt tggtctcttt
    14041 gatttatttc gtgttgacct tcagtatttc ccgtatatta gctttagttg aaaggagaat
    14101 gggtctgaat gcttaatgta aaaaaactgt caaaaagttt tggcgagaat gaagtcttaa
    14161 aatcaattga tctttctgtg aaagaaggag aagtagtggt gatcattggc ccttcaggaa
    14221 gtgggaaaag tacaattcta cgttgtatga atctgctgga agaaccaact tctggtgaaa
    14281 tcttttttaa gggacaaaac attacagccc ctgacagcga tatcaatcaa attcgtcaaa
    14341 aaatgggaat ggtatttcaa agttttaatt tgtttcccca tatgaatgta ctcgaaaatt
    14401 tgaccatcac acctgtaaaa atcaaaaaag aagaccctaa aaaagcaaaa gaacatgctc
    14461 tatcattatt gaaccaagtt ggtctaaagg aaaaagtgga cagttatcct accagtttat
    14521 ctgggggaca gcaacaacgg gtagcaattg ctcgagcatt agcaatgaat cctgatgtga
    14581 tgcttttcga tgaacctaca tctgcacttg atcctgaaat ggtcggtgaa gtgttggcag
    14641 tcatgcagga tttagctaaa aaagggatga cgatggtcgt ggtcactcat gaaatgggtt
    14701 ttgctaaaga agttgccgat cgtgtgattt ttatggctga cggcatcatt caagaagagg
    14761 gaacaccgaa agagattttt gaaaatccgc aaaatagtcg aacacaggat ttcttgaaca
    14821 aagtgttgta acaaagatgg aggaagtaag atggacaaaa gtaagattgc acgccggttt
    14881 cagcaaccag cagaaaatct gttgatgaat attgctattt tggcaaagga aaaagaggat
    14941 attatcgatt tatcgattgg tgatcctgac ttgatcactg atccgacgat tattgaagca
    15001 gcttatacag atgtaaaaaa tggtgctact aattatactg cgcctgatgg cagcaaagaa
    15061 ttcttgtcaa cggtcgtaga tttttatcaa aagaaatatg gattgagcgt tgaatgcaac
    15121 caagttcgag ctacagtcgg agcaatgcac gggatgtatc tagttttaca ggcattacta
    15181 gatccagagg atgaggtcat catccatgaa ccttattttt caccatatcg ggaacaagtc
    15241 attttatctg gaggcaagcc tgtttttgtt cctacctatg aagaaaaggg cttccagttg
    15301 gatgtggaag atttaaaagc agcgattacg gatcgtacaa aggtgatcgt catcaattcg
    15361 ccgaataatc ctacgggagc tgttttttct gaaagcacgt tgaaaaaaat cgctgaagtc
    15421 gcaatcgaaa atgaactcta tatcatttcc gatgagattt atgaagactt tgcctttaaa
    15481 gagaagttcg taccgatggc gcgatttgcg cccgataata cgatcactgt cagtggtgta
    15541 tcgaaggggt ttgcgatgac tggctggcgc ttagggtaca tgatcgcacc agcatacatc
    15601 aatcaaattg ctgggttgat caatgaaaac atcacttatt ccgcaccgac tgcttctcaa
    15661 agaggtgcaa tctacgcctt aaagcactat gaacagcttg taccgccagt tgttgaagta
    15721 ttcaaagaac gattagctta tattgagaaa agagtggcag aaatccctta cttatcttta
    15781 tcttctgttt ctggaagcat gtatgctttt attaatatca gtaagagtgg gctcgattca
    15841 gtaacgtttg tggaagaggt tttagaaaaa agcggcgtac tttttgtccc tggaaaagcg
    15901 tttggggaaa cgatcgggga caactatatt cgactagcag cgactcagcc attggaaatc
    15961 ttaggtgaag cctttgatcg tttagcaaaa ctaaattttt agataaaact gtatatttga
    16021 aaacagctat gggtatccat tagaacaaag agggaaaatg atcctttcga gagttggctt
    16081 gttgactggg cagcgtcagg atttgacaat gaaaaagtgg tagtggaaaa tcaaagtcga
    16141 ttttccacta ccataaaata ttatagactc cattttattg aaccaaaatt tttgaagtcc
    16201 tcccgctttt acgtatgcct atcactggtt actaaactcg ctaataagaa tgtttttttg
    16261 tcgatctagg atggagagag gggcaaactt tttcgatcta catacagaca gtccgatttt
    16321 tctgacattt cctattcatt tctagaaggt cagcctatta caacaaactt ttttctgtcc
    16381 tccatcatcg gattttcctg aaggtctaca tattggacac gtccgctggg gcttggtgat
    16441 cgtttgactt cttccacgaa ttttttgatt gcctcatcgt cgcccgcagc ttcgatcgtg
    16501 acagagccgt catcttcatt tttcactgtt ccggtgatcc ctaattgatc cgctacgatt
    16561 tttgtcatat agcgaaaacc aacgctttgt acacgtcctt gcacgttcat cttgattttg
    16621 ttcattttat tcaccgttct ttcttttatt tttaaattaa tgataccgac ttttttgcta
    16681 ctgaacaagt gcaatgatct aatccatggt ataatcagtt gaggtgaaaa atgtgaaaga
    16741 gatccaatca gcaaaaaatc caagaatcaa agaattaaaa aaattacata agaaaaaata
    16801 tcgtgaagaa aaaggggaat atctgcttga agggtttcac ctgatcgagg aggctgttgc
    16861 ctcaaatgcg aaggtcaacg aactttttat tacgcaacgc ggtctgactg agtgggctag
    16921 ttggcttgaa gaaaatcagg cgctttctgt tacactagta tcagaagaag tgatggcaac
    16981 tgtttcagat ctaccaactc cgcaagggat catggcagtg gtggaagctg aaaaaagaga
    17041 gataccgaat tttgaaggtg gttggttgtt gctcgacaac gtacaagatc ccggcaatgt
    17101 agggacaatg atccgcacag ccgatgcttt tggtctatct tgtgttgtac taggtaaggg
    17161 aacggcggat gtctacagta ccaaagtatt aagaagcatg caggggagta actatcattt
    17221 accgatcatc caagaggatc taacaaaact attgcctatc cttaaagagc aaggatttat
    17281 aacctatgga acagaactga atgaagaagc agtgtccctg aatgatatcc agacaacaga
    17341 aaattatgcg atcgtgatgg ggaatgaagg tcaaggcgta cgtccagaag tgctggcgct
    17401 aacggataaa aatgtgtata taccgattca aggaaatgcg gagtctttaa atgtaggagt
    17461 tgcagcgggc atcatgcttt atcacttctc cgtatagtta aggaactatt aagctttctg
    17521 cattttgcga aaacctcact agaatattac gaacactagt ggtatactaa gttcaatttc
    17581 aaagataaat ggaattcaaa aaggtggcag atcgataatt atgacagaaa aatggcgtga
    17641 agacgaagaa tatatgtctt atataaatga tctattagaa actgatgcag taaagaaatt
    17701 agcaaattac acgcaacatg tccactcgac acgattagag cattcaatca gtgtgtctta
    17761 ctatagctac aaattagcga aaaaatgggg cggtgatgca agagcgactg ctcgagcagg
    17821 ccttttacat gatttatttt actacgattg gcgtacgacg aagttcgatg aaggtacaca
    17881 tgcgtatgtt cacccaagga tcgctgtgaa aaatgctgaa aaaatcacgg agctttcaga
    17941 tctagagcgt gatattattt tgaaacatat gtggggagca acgattgccc ctcctaaata
    18001 taaagaaggt tatatcgtta cttttgtgga taaatattgt gcagtcaaag aagcagctca
    18061 accaatgaca gcttcaatga aacaaaaatg gcaacgatat ttcggtaaag aacaatcgat
    18121 ttaaggtagt taactaagta tttgacccga gtattttgaa aaaaatcctc gggttttttg
    18181 tttagacatt gactaattgg tctttttagt gtttactttg ttgagatgaa aaagtacata
    18241 accaacataa aaataaaggg ggtgatgata agatgcgatg tgtatcttat ttaaaagagt
    18301 ttgagggctt tatctaaagg cactttgata gaaagatcac ttttttcttc aaagtagccg
    18361 attttgaaag aagaaaggac tcaaagtcat ggaaaaaaca atcaaaccat atcaatatta
    18421 ttttatcaag cgagaagtcg agcagctgtt aagcgcctat agttcagtga atgatccaaa
    18481 gacagtccaa acagtccaag cattagctgc ggaaaagatc cacgaggtac ttggtcatcc
    18541 taatacagct gtaaatgaat ttatggaaaa ggtactagac gtcactctaa aaaaagaaaa
    18601 agcagaacga ctattagagg aattgaaagt gatcgttctt ccattcatac aaccgacaaa
    18661 accgcagatc gataaattat tccgaaaagt gaaaaagtta aaacaaccag attggaatca
    18721 actggattta agggaacata cttatattgg ttggaatgat ccaggtagcc aaaaaaaatt
    18781 tatgatctat tatgaagaag aaaaactaaa agggatcagt ggaacattat ctcctacaat
    18841 caacaaagga atttgttcga tttgtcaaag aacttcaaat gtttcactct ttctttctac
    18901 cacaaaaact ggttcagatg gaacatacac gaaaaaagga aattatattt gtcatgatag
    18961 tgaccaatgt aatcagcagt tggtacaatt agctcctttt tacgaattca cagaacgagt
    19021 gaaacgtgag cggtgatttc attgtatttt tctgaaaaat atgctatctt atttggtgaa
    19081 gtgaataggt gatggtgttc gcctttaaac gttgatcgta agataactaa tgacgcctac
    19141 taaagaaacg gggctacccc tttttttagt aggcgttttt ttgaggagga aatgagatgg
    19201 aaaatcgatt acctgatcca ttgaaggtga tcatctatat gatcaaatgg ttattgatca
    19261 cagcaatttt aggtattttt atgggaagtt tatccgcatt ctttctaact agtttgactg
    19321 atgtgacagc tattcggctg gagcatcctt ggttactttt tcttttgccg ataagtgggg
    19381 gactttttgc ttatctatat acgacgtatg ggggcaattc ttcccgggga aacaatttag
    19441 tgatcgatca agggaatgga ggaaatgaaa agattccatt acggctgatc ccactgacct
    19501 tatttggcac cattaccacg catctttttg gtggttctgt tggtcgggag ggtacagcgg
    19561 tccaaatggg cggggcactt gctgagaatt ttgctcgttg gctaaaattg aacaaaatcg
    19621 agcgtgagat tcttgtcatc agtgggatca gcgcaggttt tagctcagta tttgggacac
    19681 ccttggctgg gacattgttt ggattagaag tattggcaat cggaaaagtg agaaccgaag
    19741 cactgtttcc aagctttttt gcagcacttt ttgcgaactt tgtaacggaa agtttcaatg
    19801 tcaagcatac ccattatcaa atgggggcga ttcctgcatg ggacccttca cttttcttca
    19861 aattgttgct tgcgggcatt gcatttggta tcattggctg gctctttagc cgttcgatcg
    19921 tctggttaaa agcttggtat gcaaagcgga tcccccatcc agttttacgc aactttattg
    19981 gaggagcagt cgtggtactc gtagtagtga ttttacagac gcaaagatat ctcggattaa
    20041 gcttaccttt attacaagat gcatttaatg gaagtgctca tccttatgat tttcttggga
    20101 aacttggctt taccgttcta tcacttggcg cgggatacca aggaggcgaa gtgacccctt
    20161 tgtttgagat tggggcaact ttaggtgcga cattagcacc cattcttcat gtatcagtac
    20221 cttttttagc aggattaggg tttattgggg tgttctctgg agcaacgaat acaccgattg
    20281 cttgctttgt tatggggatc gaactttttg gaggtcaagc agccagctac ttctttatga
    20341 tctgtttgat tagctttatg tgttcaggaa atagtggaat ctattctgcg caaaaagttg
    20401 aagtgaaaaa aggaatcttg ttcctaccga tccttggaag aatctcacga caaaaagaga
    20461 aataaaaaga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    20521 attgcttttt aaatttttgt gaatttcgct tatttccgaa ggagttgctt ctgctcccac
    20581 cgtttattcg gagttagagt ctgtggcaaa actgattttt agttttgtcc cacgctcttt
    20641 tttattgagc aaatttgaga ataagagcag aaaatactgc aaagccaatc attaagccac
    20701 agatgataag atggcgtttt ttgtgtaaga caaggatggc aataaattct cttaagatag
    20761 cattcggtaa gtagtatagt gcagtttttg caccgattcc gtttgcattg agtttcgctt
    20821 gtcgcgcata tagtccagct ctaaagatat ggtaattatt tgaagtaaaa atcgcgcgat
    20881 aaggcccatt catttgctga tccattattt cttttgaaaa cagcatattt tctagcgtcg
    20941 ttgtggaatt cgtttctact aaaatatcac gctcgggaat ccctttttct aaagcatatt
    21001 gtttcattgc aatggcttca gctacttttt catcggttcc ttttcctcct gacatgagca
    21061 agactggcgg attcaaagtt gctttacttt gtgcccaata aaatgcgatc gccttattga
    21121 tccttttagc aagtagggga gatacgcgtt caccatcaat caagccagcg ccgagaacaa
    21181 cgatataatc ttggttatac cttggacggt tgaactgata caaaattgaa acagtcataa
    21241 aattaaagaa aacgatgagt agataagcaa aaataaacgg aatgacagca aatagagcat
    21301 ttgcccattg tgggagccaa tggactaatg tatagtccaa tatcaaaaag atagtcaaag
    21361 cgactcctaa caagagagtc aaaaggttgg ccaaagaacg ggattctttt ttcatgacta
    21421 caacaccatt ccaaaataaa aaaatgatca aaatgtagat accgattgcc aaaagaatta
    21481 atattggtat cccgatcaaa gccaataacc caccgataaa gatattttgc gtctctatta
    21541 gtagccaccc tagataacca ccaaagacaa tcaaaaacgt attaaaaagt acgccattga
    21601 ttaacctacg tttttcctta aaatagaaaa atgcaaaaat actgaaaaat agcaatggaa
    21661 tcaaaaaata aaatgtatga taaaaaactt ctaaaacgac gaagtaagtg accatcatca
    21721 taaaagtcca cgtcccacaa taaaagaata gagcgggatc gctttggcgc ttgcgccaat
    21781 agaggatgcc tgacattcct aaaatataga taactaaaaa tatcaggtct ctagtaagat
    21841 tcattgaatg aactcctttc gatttgtaaa ttttttctta aaaaagatag catcatttag
    21901 tagtaaatat atcgaatata gaaaagttag ttactaaata gtcaatcagt tttttcacga
    21961 cgtttctatt ataacgaatt atgttgaaaa aagcttttat cgaaaacata aatcttcaaa
    22021 ttaacagatt ttttagacct cgatcatgat gaagactttt gtagatacaa ataactgtaa
    22081 cattgaaaat gaatgcgaaa acgatatttt tttttattgg aaaatatcgg tcaagcaata
    22141 aaccagttgc ataataaaat gttaacggca acaaaatctg tggaaggaat gaaccggatg
    22201 atttgatgga aaaaacaaga tagacaagaa gtatacatag taaaacgtgg aacaagtagg
    22261 ccagaatgaa aaatcagttt ttatccactc gcttttctat acattcactg tcacaaatat
    22321 aatcgaaagt aattctattt tgagataaag tattttatag gagatagtat atgaacaaaa
    22381 aaagatttaa acgtattgaa agaaaatttg cattaactta tgaccaagct aagtatttga
    22441 aaacactatt tccaaaacag gtagttcggg ataaatatag tgatcatttt atttcttcct
    22501 tgtattttga tacaaacgat catcactttt taaaagaaat cactacagaa aagtcctatc
    22561 gagaaaaatt tagaatcaga aaatatggag atcctcagga catatcgcct atctacttag
    22621 agataaaaag aaagtttcaa ggaatgacag ataaacgtag aataaaatta gcgcatcatg
    22681 agctcgatga tatgttggaa aatatcgaat tggcagaaac cacattcagt aatttagaag
    22741 aagagtatat ttttaatgaa atacagtggt tgttcagaag gttggcattg gagcccaaag
    22801 tatatatcta ttatttaaga gaagcatttg taaatagaga ttcagaagat ttgcgaatca
    22861 cttttgatca aaatatccat taccgtctaa tgaacttaac gacctgggaa cactgtacag
    22921 aagacaatct ggtagcgcca gaaatcgatg tgctgatgga agtaaaaacg acaggagaaa
    22981 tacctgaatg gttacaggta ggattggata ggatgggtaa aaaagagata aaattttcta
    23041 aatataaaca agtctataaa agatatatcc aagaggaccc gctctatgtt caatagtgtc
    23101 ttaaaaaatg agccttctca actcacacta gatgatttct ttttatgtat ggtggtgtcg
    23161 attttgctag gcattctgat tgcttacacc catatgtatc aaaacaactg tacaaaaaac
    23221 ttcatgtttg ccttgattgg gttacctact ttagtacata ccgtcatttt gttgatcaac
    23281 ggtaacttag gcacggggat cgctgttcta ggcgcattta acttagtgcg atttcggtct
    23341 gctcaaggga attcaagaga tattttcagt attttttggg caacgtcgat tggtttagcc
    23401 acagggacgg gatatattgt ctatgcacta ttcgtgtcta gtatagttgc tgctatccaa
    23461 gtcatcatct tcacgcttcc aatcaaaaat aaacatacag tttttaatac tgaaaaaacg
    23521 atcaagctaa aagtttctaa atccttgttt ctttcgagta ttattgaaga aaaaattgca
    23581 gaatatacag aaaactgcaa tttaaaaaaa gtaaaaatca ataaagaaac ttgttcctta
    23641 cactatatag taaaagtaaa aacgagtatg cttgaaaaag aattggttcg aacgatcatg
    23701 gatcatccag aagtcatttc gatcactgta ggtatggata aatcgaataa attattgtga
    23761 taaccaagaa agtacttaac aatgaagtaa gaccccagtt ccaaagttta gcggggacac
    23821 ttcaataaag tactttcttt tttgtcttca gcaactactg gtaaaactga gagcaagctt
    23881 tcgtgagagt acagcgataa agatcaacct ccctttttat ctcgagcaat ttccttgcaa
    23941 ggctgtttcg ttgatcttaa acgagttagc aacagtcaaa gatgcgatgg ccataaaaaa
    24001 attgcattaa gttggctggt atctgatagc tgggaatcta aggaagacta tccattctgt
    24061 ttcttaaatt ccgctagttg atagatatca gccaattgtt ccagatgatc aacatcaaag
    24121 ataaccgaaa gatgtttcct tttattccca tttgcttgaa gagtttttat cttctctttg
    24181 gttccttcac tcaatgagag tgtgactaat ttctccctcg ttttttctgg gaaatgaaag
    24241 gatactttga aaaatccgtc ccaaatagaa agccagaaga tcggttttac tttctgagag
    24301 tgggacaaaa gtaagaaaca cttttgtctc attctttaaa aacgaataaa cggtgggagc
    24361 agaagcaact ccttcggaaa taagccaaaa ttcacaaaaa tttgaaaagc aattttcgtg
    24421 aatttcttct tatttctcgg agttaaacac ttctgtccca acctcttgta ttcacctttt
    24481 gatagccaag cttttccatc attataaaac cgcaaatttc aaaaaaaaac gaacaataca
    24541 aaaagcgtat tgctcgtttt tctcttcagt ttctcacacc taaacgactt ttgtctcgac
    24601 ctctttttgg tgactattca tgatgttaaa ataaattact gaaaaaatct gtgacatggc
    24661 gccaaccaat gacaaaaata ttagcttttt caacagtaga agtggtaatg atgtctgtcg
    24721 aaggctcttg gtttgcttcc aaatagccta atttgtcatc agctaattgg atcgttgctg
    24781 agccaactgt agtgcctttg ttgataggag cggtcacttg gtcatccgtt actttctttt
    24841 tatcaattgt tggagtaatc gttaattttc cagtatccat tccattttcg acccagactt
    24901 ttacagggct ttttagcgca acattgacgg aggtttcttt tccatctttc actttgatgt
    24961 cttttaggct tgggatgctt gcatttgctt ttccaagttc tttttctgac cagttgtcat
    25021 aacaataatc catcaatcgt gatgtttcat tgaatcgggc acttggattt tcttcatggt
    25081 tatctgcatt taaaacgact gtgatgattc gtcggccatc tttgttgatc gttccgacaa
    25141 agcatgcacc cgcaaaatca gttgtgccag tttttaatcc atcgacacct tcgtgatagt
    25201 ttacaaaacc aggtagcatc cagttccaat tgaccatttc aaccggtgac tgtgtgtttt
    25261 cgccgaatgt ttgatttgtg gtactcgtga tttttaagac ttcaggataa tctaagatga
    25321 ggtgacgagc aacaatagcg acatctttcg cagacagttg attttcatct gtttcaccag
    25381 tattctcagg tcgattctca cctaggtaag aattgtttaa acctgatgca ttgacgatcg
    25441 ttgcatcttt gataccccaa tcttttagtt gtttcttcat tagctcaaga aatttacttt
    25501 cactgcctgc aactttttca gctaaagcaa ccatggaagc attggctgat tggatgaaag
    25561 ctgagtcaac aagttctttc actgtataat cattttcttt atgcaaagga acattcgata
    25621 gatccggcac gacacttaat ttttctgcat aatccgaaat ggctacctta tcttcccaag
    25681 ataatttacc ctctttgatt tgttcaagta cgatatatag accaatgatc tttgtgatgg
    25741 aagcaattcc catcgttttt tcgccatcct gatcataaag gatttttcca ctttctgcat
    25801 ctacggcaaa ggcagctttg gcatttattt taaatgcgtc gtctgcttga acagttgcgc
    25861 taggcaagaa agaagtgata cataagataa gacttgctaa tagtacaata aaacgggtat
    25921 tttttttgaa catctttgtt ctcctcattt ttaaattgat tcttacaact tagaatatta
    25981 tcagagagaa caaaaggttt caatggttct gaaaaaaacc ttataaagaa caagtcttat
    26041 taatgaagaa tcatagaaag ttatcttttg ggttcattga cacgaacttt gactggctga
    26101 acttcgggtt cggttaggcg tgatgctgca aaaagggcgc ctgctgagag tgcgaccaaa
    26161 ctaaaaatca tgaataattg cataagatca cctcgttctt ttttgttttt ttatagtata
    26221 gcaagagagt ttgaagctac tgttaaggaa atgagcatat aattgatgaa agtatgaaaa
    26281 aaatatgaag taggacaaca caactaaaaa tcgatcaggt atttctctgg tagggattcg
    26341 ttttattgta ttgataaaat gggatgtggt agactttatt tacagataca aagaaatgat
    26401 ttttttagga ggggtacgtt tatggcaaaa aaagaaaaag aattattatc acaattgact
    26461 tcagaaggta ccaaaggacc gtttgtcacg attatgttaa acacccatgt tgcgcatcaa
    26521 gacgtagaaa aagatcaaat caaattcaag aattttgcaa aggaagcgaa aaagcgcttt
    26581 gaaaagaaat actctgagca tgattggtct ccattccaag aaaaaattac tagtttgttg
    26641 gacgatccgt ctttctggcg tagtgccaca acgagtgtag ctgtcatagt atcaccagaa
    26701 caagtgttta tccaccgttt gggtattcct gtagatgacc agtattatgt gagtgatcta
    26761 ccttatttat tagcgatcat caaaaatgga catttcaact attcatacta tttattagga
    26821 ttgaatcgtg attcgatgaa attgtacgaa gtaacgaata aacaagtcaa agaagtggct
    26881 ttgcctgaag atgcaccaag agatgtcgtc actgctttag gatctgaact aacaggaggc
    26941 gggttgaact acgcatcaaa tggtagtgaa ggtacgacgt tccatggagt caatcctaaa
    27001 gatgaagaag tcgaaattga ttgggtcaat tattatcaag cagtcgatga ctatttgaaa
    27061 gatacatttg aaaatccaga gaaatggcct atcttcttat ttgcattacc agaaaatcag
    27121 acgatgttca aaaaagtggc gaagaaccct tattatcgtt ccgatgttgc gatctcagct
    27181 tcacctgcac aactttcatt aaaagaaatt gaagcaggcg caaaaaaact atcgcaagaa
    27241 ttagctgtca aagaagcaag tgactacaat aagttgcttg ataaaaagtt cattgatcaa
    27301 ctcgttgata ttataccggc agcaaaagaa gggaaaatat cccatttatt cattgctaca
    27361 tctaatttag cagatggttt tggcgaagat ccagaaacag aatatgaccg ccgtcaggtg
    27421 ctgaatgaac tagcagataa tgtgatcacg aacggtggtc aggtctttat ccttgaacaa
    27481 aaagatgcac cagacgaaaa gagtttagtt gcgatcttac gttattgagt gaaaactacc
    27541 aaagtagatg aatggtcagg cgttgataga agtgtatagc tccgagagaa ggagtttcct
    27601 ctgtttcagc cgttggattc atcttgagcg agagacaaaa gctagctttt gtctctcgct
    27661 cattattttt gtgctttagt cccattggct ttgcgagggg aagatacttt tctcaaaaaa
    27721 acgaaccttg cagttgagag ggggatagat gcgcatacta atttttatca gttaaaaaat
    27781 gatgtggatc attagagaat tccaaagttc gttcatcctt tttcagataa taaattgaaa
    27841 ttttttgcaa aagtgaaaat agcaaataga aattttctga atcttatagg ggtgatttcg
    27901 agaaaacgtt cgtttatttg caaatatatc ttctaaataa gagaataaat acaaaaaaat
    27961 aaggaaaaac tctggcaatt ttgtaaacgg tatgatatag taagaatgta gtttttgttc
    28021 ggtaatctgt ggtagcttgt ttcaaataaa catcttccag atatacacca ttcaagtaat
    28081 tgcaaaatat gtgttgatac acaaggagga tttttattat ggaaacaggt acagtaaaat
    28141 ggtttaactc agaaaaagga tttggattca tcactgcaga aaacggaaac gatgtattcg
    28201 ttcatttctc agctatccaa ggcgacggat tcaaaacttt agaagaaggc caagcagtaa
    28261 ctttcgacgt tgaagaaggt caacgcggac ctcaagcaac aaacgttact aaagcttaat
    28321 ttacctatat taaaaataaa agtccagaca tttatgtgtc tgggcttttt tgtctttttt
    28381 tccttaaaaa gcgatgagag tatttttctg cactttgtag tataatttca aaagaacatt
    28441 agaggcagga gaagaggaag atattttgaa accaagaact tatgcagtcg ttgatatcga
    28501 gacgactggg acgaatccaa aagaagatcg gatcatccaa tttggctgcg tcatgattga
    28561 agctggtcgg atcacttcac gtttttcgat cgatattcat ccagggcgga agatttcaaa
    28621 acaaatccag catttaacag ggatcacgaa tcaacgcgtc caaaaagcgc cttattttga
    28681 agatgtcgcc caaacgattt acaatctttt ggcagacacc atatttgtcg cgcataatat
    28741 ttattttgat tataattttt tgaaccatga actcatgcga tgcggacttc ctagtttgaa
    28801 gataccgggc atcgataccg tagaacttgc acaaattttc ttgccgacgg aaccaagttt
    28861 tcgcttagct gatttgtcag aaagccttgg ctttattcat gagaatcctc atcaggcgga
    28921 tagtgatgcc gaagtgacag gtcagcttct tttactgatc gaagaacgaa tgaggaaatt
    28981 gccgatcatc acattagagc agattgcacg attaagtagc catacaggaa tggatacgag
    29041 tcgtttcatt taccacgtgc ttgaagaaat gaaagaaaac ccggagcccc ttgatctctc
    29101 attagaaatc atcgatgggc tagctttgca gaaaaaagaa gtggagctat tcacatccgt
    29161 ccattatggc gagcggacat atccaagaaa aaaacaagca aaagaaaaaa tgtttggtaa
    29221 aacactgatg tatcgaaaag aacagaacag gctgatgaat gctgtctacg accatttcac
    29281 aaaagatgaa tcaaaagatt tgatgatcga agcagctaca ggaatgggca aaacgattgg
    29341 ttatctgttg cctttgagtt atttggctac acctgaaaaa ccagctgtca tcagcaccgt
    29401 ttcgcttgtt ttgcagcagc agatcatgga aaaagacatt ccattactga atcaattgct
    29461 agatcagccg atacaacctg tgattatcaa aagctatcgc cactatattg atttacagcg
    29521 ttttaaaggt acacttgtgg aaccgccggg acaaaaacaa tatgctttgt accaaatggc
    29581 tgtcttagtt tggctaacac aaacgaagac cggtgatttg gatgaattac atttaaccaa
    29641 tctgaaccat gcattctttg cagatgtcgc gcacagaggc actgggtttt tagcaaggaa
    29701 tcagtcattt tacaaacaag actttgtgcg tttcttgcaa aagaaaatca aacaaagcaa
    29761 ttttttgata gtcaaccatg catttttagt acaagaaaca cagcgaaaag aaccattgct
    29821 tccagctagt ccttatttgt tgattgatga agtgcatcat ttgccggaaa ttgctgaaaa
    29881 agtaaatgac aaacgactaa gtattaacta cttccataaa caaattcagc aactggaaga
    29941 gccgaatcag ttatttgacc gcatacaaag ttatttgccg aaggaacatg aagccttgcg
    30001 ctggttgtcg atttatcgag aagaactctc tgcactatgc gaagcgcaga tttgcgtggc
    30061 agagggatta atgaacttag taagcagcaa gcaacctaac ggacaatatc cagaagaagt
    30121 catgatgaca aaagaaatgt tggatcagct gccactggaa tctggaacag caatggatca
    30181 tctgcaactt ttttatcaag agatccaaca actacaatca cagatgcgct tgacttttga
    30241 gattatccaa gaaagttgga ccaaccagat gcggatcgat ttagcaagtt tatatagctt
    30301 atttgatcaa ttagacaagc aatacttgct gatggaccaa tggctagaag actggcgaga
    30361 tcatttgatc cattggttcg ttcccgggaa tacagcacag cagatgattt ttcatctcct
    30421 tgattttcaa gcagctatgt tgccgtcaac tgtgtggtat cctcgatatg accatattct
    30481 ttatattgga ggaacactga agatcggacc taatcgacag tatctctcta aacgacttgg
    30541 cattgaagca gcacctctta aagtgattgc ttctccttat gattatgaga aacaggcaag
    30601 aatctatgtg ccgaaagaag cacctgcagt ccatcaagtc agcaatcaag catatatcga
    30661 ttatttagaa gcagcactca caggattgat tgaacaagaa gatcggccga tccttgtttt
    30721 attcacttct catgatgttt tgcaaaaggt atatcagaaa atgcatttgt ctatgctaga
    30781 aaaaggacgt gaaattcttg ctcaaggatt tggcgggagt cgggaaaaac ttttgaaacg
    30841 cttcattttg tcagaaaaaa gcgtcttatt tggtgctgac agcttttggg aaggcgtaga
    30901 tttacctgga gatgctttgc agattgttgt agtcacacgt ttacccttcg acaacccgtt
    30961 acgtccgatg gtcaaagcta ggaatgctta tctggaatca gaggggatca atccttttta
    31021 tcaggaatcc gtgcctaaag cagctttgaa gttacgccaa gcattaggtc gtttgataag
    31081 gacggagaca gatcgtggcg tattacttat tttggatcgg agattagtca ctgcaaaata
    31141 tggtgcacga atcatccatg cattaccgaa aaaagtaccg ataaaagagc tagctataga
    31201 tgaaatctat caggatatcc atgaattctt aaagaaaaat gaagaaggaa gttaaaggtg
    31261 ccgtcttttt tcatcattaa aatctgctat aatgaaaagg aatgaagata aaggagcgac
    31321 gacccttgaa gaaaaaacag acaaaggaaa aaacgattaa tcgcgtgttg cttggcttga
    31381 tcgtattttt attagcggtc atcgtgatca gctcgatttt ttacttgcgt tccagtcgtc
    31441 cggcggtcca ggcgaaaaaa gaagcagtag aaatcgcaaa gaaatatgca aatctcgagg
    31501 aagccgatca tttttactgg tttactcgaa agaaaacgta cttttcattg acgggtacga
    31561 atgataaagg aaaagaaatc gccgttatca ttcctaaatc tggtgaaaaa gtaaaagtat
    31621 tggatcaaac aaagggactg actgaaaacg aagcaaaaca gacaattgct gcggcccatc
    31681 ctgaaatcca aatcgaaaaa gctgctttag ggatgtatga tgatcagcct gtgtgggaag
    31741 tagtaggaaa agaaacaaat gggaaactga attattacct gattggcttt gaatctggag
    31801 atgaagtcaa gacaatcaag ggaatttaaa aggtaaagga atgaatggga tggagttatc
    31861 aaaaagaaca aaacgattag aaccgtctgt cactttggca gctgcggcaa aggccaaggc
    31921 attaaaagca aaaggcagag atgtgctgag tttgactgtg ggagaaccgg attttgcgac
    31981 accagaaaac atccaagaag cagcaatcga agcaatccgc aatgggaaag ctagctatta
    32041 tacaccaaca gcaggaatcc cagagcttcg acaggcaatc gttgattatc tgaagaaaaa
    32101 ctatggactt gtctatgaac cttcacaaac agtcgtaaca gacggagcga aatttgcttt
    32161 gtataccctt ttccaaacga tccttgaccc gcaagatgaa gtcatcattc ctgtgcctta
    32221 ctgggtcagt tacggtgagc aagtaaaact ggcagaagga cttcctgtct ttgtgaaagg
    32281 ggaagaatcc aatagctgga aagttactgt tgagcagcta gaacaagcac gttcagaaaa
    32341 aacaaaagcc gtaatcatca attctccatc gaatccgact ggaatgattt atagcaagaa
    32401 tgaactgcag gcaatcggtg aatgggcagt aaaaaatgat attttgatag ttgctgatga
    32461 tatttatggc cgtttagttt ataatggcaa tgagtttact ccaattgcaa cgatttcaga
    32521 agcaatcaaa aatcagacca tcatcatcaa tggcgtgtct aaaacatatg cgatgacagg
    32581 ctggcgcatt ggttatgcgg tggggaattc tgagatcatc aatggaatga ttgctgttgc
    32641 ttcccagtca accagtaatc cgacagcagt cagtcaatat gcagctgttg aagcattgaa
    32701 aggcgagcaa gatacagtgg aagaaatgcg gaacgccttt gaagaacgcc tgaatacgtt
    32761 atatccgctt gtggcagaac tgccgggtgt ttctcttgaa aagccacaag gagcttttta
    32821 tctttttcct aatgtaaaag aaacgctacg catgtgtaaa tatgaaaatg tcacacaatg
    32881 ggtagaagat cttttagaag aaacaggggt agcgcttgtg actggcgaag gctttggggc
    32941 accagaaaat gtccgcatga gttatgccac tgatcgcgct acgttagaag aagcggtacg
    33001 cagaatcaag caatttattg agagtaaaag tcaaaattaa tcctgaagaa attcaaggaa
    33061 tataaaagga gagtcatacg tggaacaaat tcaaatcatc gattcgaaaa accatgtggg
    33121 agaaacagta acaatcggcg cttgggtcgc aaataagcga tcaagcggaa aaatcgcatt
    33181 tttgcaatta agagacggaa cagccttttt ccaaggtgta gttgtaaaaa gcgaagtgcc
    33241 ggaagaagtt ttccaaacag ctaaaaatct acaccaagaa acatccgtgt tagtaactgg
    33301 agaaatccgt gaagacagtc gttcaaaatt tggttatgaa atcggaatta caaatgtgga
    33361 agtggtaagc gagagccacg attatccgat cacaccaaaa gaacacggaa cagacttctt
    33421 gatggatcat cgtcatttat ggctgcgttc ttcaagacaa catgcgatta tgcagatcag
    33481 aaatgaaatc attcgtgcaa cgtatgaatt ctttaatcaa catcattttg tcaaaatcga
    33541 tccgccgatc ttgactggtt ctgcaccaga aggcactacg gatctattcg aaacaaatta
    33601 ctttgatcaa aaagcttatt tatctcaaag tggccaatta tacatggaag ctgctgctat
    33661 ggctttcgga aaagtctttt cattcggtcc gactttccgt gctgaaaaat caaaaacacg
    33721 acgtcatttg atcgaattct ggatgatcga accagaaatg gctttcactc atcaagaaga
    33781 aagtttagaa gtacaggaac aatatgtagc attcttagta caaaatgttt tagatcattg
    33841 cgaatacgca ttagatgtac taggacgaga caaagaagtc ttaaaaactt atacaaaatt
    33901 gccattccct cgtattactt atgatgatgc gatcgaatta ctgaagaaaa atggatttga
    33961 tgacatcgaa tggggcgaag actttggttc acctcatgaa acgttcattg cgaattcatt
    34021 cgaacagcct gtctttatcc taaactatcc aaaagccatc aagccattct acatgaaacc
    34081 acatccaaca agagaagacg tggtgatctg tgcagatatg atcgcaccag aaggttacgg
    34141 tgaaattatt ggtggaagcg aacgggcaac agactatgat ttcttgctag aacaaatccg
    34201 taatcacggt ttggatgaaa aagaatattc atggtatctt gatctacgta aatacggttc
    34261 tgttccgcac tctggttttg gtctaggcct tgaacgaaca gtcacttgga ttgctggtat
    34321 cgaccatgtg cgtgaagcta ttccattccc acgtttgttg aaccgaattt atccataaga
    34381 ggttgtgaaa tcagtgcctt gacctgagta ttagagaaac aaaacggaaa aatagcttct
    34441 catattttac tgttttttga gctaatatcg agggtcagct gattgaacac cgtttatatc
    34501 aagaggttgt gcgtctgccg ttcagctccg agtcacaagg agtaatttca aaaaaacatt
    34561 ttttcatgtt tgatgactat tgtgacttgt gaccaaggag ttggccggag agcaacgttt
    34621 atttaaaaaa aagaactgcg aactctcgca gttctttttt tttaaatacg ggataaagga
    34681 agtttccagc agataagaga agaaagcaaa agaaattgct atttgattca caaggttcta
    34741 taacgaaatc gcaatcaatc aaacatatac aaagaaggtt attcaaatgg aaatctgatt
    34801 tgatgatgct tctgctgttg aaataaagca gcatctagca cctgaaaaca aactaatagt
    34861 aacattcgaa gatggagtag gtccgtattt tcagcatgca atgatccata tgtaagttca
    34921 attcaccctt gaaatcatcg gaccagataa agttatggcc aatctatccc aagcaatcta
    34981 gaagagatat tagtaaagga acatttactg gaagtcttag atgagcctat aaatatatgt
    35041 tctgatggcg tcatcaagtg gtctgattga cggaagctta ggtgtgatcg atctctctgg
    35101 accaaacaat gaaggaatca aggggaatcc agcacgttaa gaaaaaaata gcataaaaaa
    35161 ctagacactt gatgagcgtt cattcaattg tttgaaggga ctaggctggt gtcatttttt
    35221 atacatatat tttctctttc gtcttgatag ggcttatggg ataatataag gaagaaaatc
    35281 atcgttttat tcaaaaagga gtttttgaca aatgaaaaaa cgtatattat ttatcggtga
    35341 cagcattaca gacgcaggaa gagaccgagc agaagcaact gatctaggta aaggttatcc
    35401 acttttgatt gccggaaaac taatctccca atatccaacg atggaatttg aatgcatgaa
    35461 ccgtgggata ggaggagaca aaattgcaga tctgcaagta cgctggcgag aagattgttt
    35521 agctttacaa cctgactatg tatcgatttt gatcggaatc aatgatacct ggcataatac
    35581 tagcgatcag gctatattcg gaactgagaa atctgctcag caatttgaat ccgtttatcg
    35641 aaaattacta aatgaacttc aaaaagcggg aatctgcaac atcatcttga tggagccgtt
    35701 tgtacttcct ataaccgacg accgtcatgg ctggcgtaaa gacttagacc ctaaaatcca
    35761 aattgttcgt aaacttgcta aggaatatgg cgcattgctg gttcctttag atggcatatt
    35821 caatgcttta gggattgctt atggtttttc gaaatataca agagaagatg gggtccaccc
    35881 aacgattggc ggacatgaga tcattgcaca aagctggata gatgcaattg ccgcagtcga
    35941 tttttttagt ggagataaaa atgagcgaaa gaaaagtgag aaatgatgga aatacggtgg
    36001 gcagtaaaag aagatctacc tcaaatcaat cagctgatta ctgatcatcc agaatcattc
    36061 gtaaaagcaa aaacggcagc tgacctaaat gataagaata aactattgct cgttgctgta
    36121 acaaaagaaa aacggattat cggttttgcg caagcggacg tgcaacagtc agaagaacgg
    36181 gtgggtcagc gaccatcagg aaaactaatc caacacacat tagtaacaaa agaaaatacg
    36241 gaagaaatcc aaaaaaaatt tgcagaatat ttgatgctgg aagctaaaaa aagaagaata
    36301 aaatcaattt ttgcccagtt ttaatcaaaa acagtaatag aaggagaaat acaatgagtt
    36361 gtttaggaaa tgtcatctgg tttatattcg gcggactaat cggtggtctt agctggttag
    36421 cagcggggat actttggtgt atcacgatca taggtatccc aatcggtatg caatgtttta
    36481 agatcgcctt cctttctttt gcgccttttg gcaaagaagt aatttatcac cagtctggca
    36541 tgtcattgat cgttaatatc atctggctga tcgtttcagg cctaccaatt gctctcgcac
    36601 atgtcgtcag cggcatcctt ctatgtatca cgatcatagg gatacctttt gccaagcaat
    36661 ctttcaaact tgctcgattg gcgttgatgc cattcggtgc agaaattgta taaaagagaa
    36721 taataaaggt tagataaagc atagagtcag gtgggataca tgtatttcac ctgactctat
    36781 gcgttttcct gtataagttc ttccatgtga cttttacgtc ttttatttat atggggttac
    36841 tgtttatgga aagttgaaat aattttcatt aaagaactct ttctatattt ttgaaagcgt
    36901 agtggagctt cgtatatctg ggaaggacat tttgcaggga ttgaaaaaag atattgtcct
    36961 atcgaaaaat taatgaattt agacaaattg ccagcaacag gcttcaaact tagttgctat
    37021 cctgtcaaga tcaagaaggc aagtgcgggt tggataagag cagtggcaat gatcgaagag
    37081 aaaaagaaag aataagccaa aacatctgta tggaagaaat tttccgatgc agatgttttt
    37141 cattttttct ttcggatacc ttgacatatg aataaccatt catttataat tagttatgaa
    37201 gagatattca tatgttgagg cgaggaggtc attaaaatga aaagttacag attagaaggt
    37261 ttagattgtg cgaactgtgc aatgaaaatc gaaaaaggag ttcagaaaat caatggagtc
    37321 aaggaagcaa cggtaaactt cacttctgga aaactaacga ttgacgcgga ggaagatcat
    37381 ttggctacga ttgaacaaga aacaaaaaaa gtagtgaagg aattagaacc agatgtcaaa
    37441 gtaacagaaa tagataagga aaaagtatct gaacatggaa atgaaaaaga gagaaatacg
    37501 ctgttccgga ttttattttc ccttgcaggt atcgctttat tgctattatt tgattttaat
    37561 gaactaattc gcttgatagg gtatttattg atttatctgc tgataggata tgatgtagtg
    37621 aagaaggcag tgatgaatat cgtcaaagga aaaatctttg atgagaattt tcttatgtct
    37681 gttgctacga ttggcgctat gttgatcggt gaatatccag aagccgtagc agtgatgcta
    37741 ttttatcaaa tcggcgagta tttccaagga ttggcagtca gtcattcaag aaaatcgatt
    37801 cgcgaattga tggcgatccg tccagaaaca gctcacgtgc agacggcgga aggattgatg
    37861 accgtgaacc ctgaagacgt gctgatcggt cagtttgtac ttgtgaagcc tggtgaacgt
    37921 gtaccgttag atggaacgat catcgaagga gaatcattag tcgatacgtc agcacttaca
    37981 ggagaatcag tccctaaatc tgtatatgtt ggtgaaacag tattaagcgg atttatcaat
    38041 aaaaataaac catttttagt tcaagtagaa aaatcttatg aaaattctac tatcagcaaa
    38101 ttattagaat tagtagaaaa tgccagcagt aaaaaagcac cagctgaaaa tttcattact
    38161 aaattcgcgc gctattacac accagtcgtt gtaggattgg ccgttttatt agctgtttta
    38221 cctccacttg ttgtttctgg agcggcgttt tctgaatgga tctatcgagc attgacattt
    38281 ttagtgatct catgtccatg tgcattagtg atttccgtgc cacttagttt ctttggcggt
    38341 atcggaggag caagtaaaat cggtgtatta gtaaaaggtt caaattactt ggagttgctt
    38401 gcgcaaaccg agacagtggt attcgataaa accggcacat taacgaaagg cgacttttcg
    38461 atccagacga tcgatacaaa aatcgatcca aagatattca tgcaatatat tgcgagtgca
    38521 gaacagtttt ctacacatcc gattgctcaa tctgttttag aaggctacgc aggtccgctt
    38581 ttaccaacag ctaatatcca ggaatttgca ggtgaaggga tcttagctga agttgatggc
    38641 aaacaagtat tagtaggtaa tcacaaattg atggaaagat ttgaaatctc ttttccttca
    38701 agtcaagaaa tcggtacatt gctttatcta gcaatcgatc aaagttacag tggttatctt
    38761 gttattgcag ataccttgaa agaagatgcc gtagacgcat tggttcaatt aaaacaggct
    38821 ggtgtgaaaa acacagtcat gttgacgggc gactcgaaaa aaattgctga tcatatcggg
    38881 aagcaagtag gcgtggacaa gatatatagc gaattgcttc cagaagataa agtgcagcgg
    38941 ttagaggaaa tcttacaaag taacaataaa aagaccgcat ttgtcggaga tggaatcaac
    39001 gatgcaccgg tgctagcaag agcagatgta ggaatcgcaa tgggtggatt gggaagcgat
    39061 gctgcaattg aagcagcaga tgtagtcatc atgaatgatc aaccatcgaa gattgcagaa
    39121 gcaatccatt tagcaaagaa aacattgaaa atcgtcaaac aaaacattgt ctttgctatt
    39181 gggataaaaa ttttggtact ttccttaggt gcttttggtt ttgcatcaat gggagatgca
    39241 gtatttgcag atgttggggt gacagtatta gctgttttga atgccatgcg aagcttgcat
    39301 gtaaaaaata aacgttgatc aatgaagagg acgaatgaac gtcctctttt ttttatatcc
    39361 ttttcttaat gaaaaatgta aagaaaatag ataggattat aaaataacta atgtttttaa
    39421 ttttgcagaa aattcaaaat agcttgaaat tcaataaggt caatgaaata aaagaaaata
    39481 acttattttt ttgatggaaa aaatatgaaa aaacgatgtg tattaaataa aataatacac
    39541 acataaaata acactgtctt gtaagtttgt taaaaattga atatgattac atctggataa
    39601 caaattttta tcataaagga gagaacgaat gaacaacaaa caattacgct ggtttactgt
    39661 tgcgttaatt gcatttaaca tggtttgggg catggggaat gtagttaata actatgctca
    39721 gcaaggagta tcagttgtca cctcttggct attgattttg gccatttatt ttattccata
    39781 cgcattgatc gttggacaat taggatcagc cttcaaggat agtaaaggag gcgtcagttc
    39841 atgggtcgaa aatacgacat ctaataaacg actggcatac tatgccgctt ggacttactg
    39901 ggtcgtccat attccttatt tggcacaaaa gcctcaagca attctgattg ctggtggttg
    39961 ggcagtccaa ggaaatggaa atatcgtaaa tacgatgtca gtacaaatgg tagcagttat
    40021 ttcacttatt atatttcttg cctttttata tttatcaaca aaaggacttt ctactttgaa
    40081 agtgattggc ggactggccg gaacagcgat gttcgtcatg tcgatcttat ttattctttt
    40141 agctgtaacg gcaccgttca tcaatccaac gatggaattt gccacaccac atatgaatca
    40201 agtgaaaaca tatattccaa catttgattt cagttatttc acaaccgttt cgatgctagt
    40261 atttgcagtt ggaggggcgg aaaaaatatc cccttatgtc aactctacaa gaaatcctgc
    40321 aaaggaattt cctaaaggga tgatcttctt agcagcaatg gtagggattt gtgctgtatt
    40381 aggctcagtc gcaatgggta tgctgtttgc tagcgataat atccctaaag acttgatggc
    40441 aaatggggca tacagtgctt ttcaaatttt aggtaattac tatggtatcg gtaactcatt
    40501 gatgatcatt tacgcattga caaacatgat tggacaaatc tccgcattag ccttttcaat
    40561 tgatgcacca ctaaaaatct tgttagcaga tgcggatcca gaatttgtac caaactggct
    40621 acgtaaacgt aatcgtaaag gtacattggt gaatggttat cttctgacag gtatactagt
    40681 aggaatcatt attttgttgc cgatctttgg tatcaaagat atgaatgaat tagtaaaatg
    40741 gttgacgaac ttgaactcag ttgtcatgcc aatgcgttac ctatgggtgt tcttggcata
    40801 tatgatgttg aataaagctt ataagaaatt caattctgaa tataaatttg ttaaaaatcc
    40861 aaaaatcggt tttatctttg gtgcttggtg ctttgccttc actgcgtttg catgtatcct
    40921 aggaatggtg ccaaaagtag attatactgc tgatcctaaa gcatggtggt tccaattatt
    40981 cacgaatatc atcacaccaa ttgtcttgat cttattaggt atgatcttgc cagcaattgc
    41041 tcgtcgagac aaaaacaaag aaattactga accagaaaca gtggttcagc cttaaaatag
    41101 aaatacttgt tacaagaggt tgtgacagaa gctacttctg gaacaccgtg aatcagaggt
    41161 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gctcctcata
    41221 ttttgccgtt ttttgagcta atgctgaagg tcagctgatt gaacaccatt tattcgaaac
    41281 aagggacttt gacacgactt ttgtcacagt cccttgtttt tttgcggcgt tataagtggt
    41341 atgatcgaac caaattccaa agagaagtga gggagaagaa tgaagatcat cgtgattgga
    41401 caaagtgggt cgggaaaatc gtccctcgca aggaagatca aagaaatcac cggctttccg
    41461 cattcaggca aaaaagtggt ttttgcaaga acagcaatcg tttatagtat ctaatccgtc
    41521 atggatcgtt gaaggaaatt atacttctac gttagaggaa aggattaaag aagcagataa
    41581 gatcatttgg cttaaagtgc cgcgttatct cgccatctac cgagtagttt ggcgttctct
    41641 aaaaagaaag ataaacaaaa aatctcggcc agatatgcct gaatcatttt cggagaaact
    41701 aaacaggaaa tacttagaat ttttatcttt tatctggcat tttgaaggaa acaatgaacc
    41761 aaaaatacag cagctgatcc aagtagctaa tgcaagaaat aaactgatca tccttaaaac
    41821 aagacgagag aaacaagaat ttttaactaa attgaaaata gagagcaaaa ataattgaca
    41881 gtttttgttt acaagcgtaa tctgtattca taagctaatg attacatttg taaacgaaag
    41941 gtgtgaaata aatggaaaaa gtcaaaacgc cagtaaagat cagtgattca gagtgggaga
    42001 tcatgcgtgt tatttggaca cttggacaga cgacagccca agaaatcacg cagatcttag
    42061 ctgattcgat ggattggaaa ccagcaacga tcaaaacttt gctgggacgt ttggtaaaaa
    42121 aagaagtgat ctggacagaa caaaaaggaa aaaaattcat ttatcatcca gctgtctcgg
    42181 agatggaaac agttcgcagt gcgacggaaa atcttttttc ccatatctgt gcaaaaagga
    42241 ttggagagac gattgcggat ctagtagaag aagctacttt gactcaagaa gatatcaatg
    42301 agattttgaa acaactggaa caaaagcaac cagtagatac gatcgaatgt aactgtatcc
    42361 caggtcaatg tgaatgtaaa caacatcaat aggaggaaga aaaaatgaaa caacaatttt
    42421 caatcgaagg tatgagctgc aatcattgtg cagcaagagt agaagaaaca gtaagcgcac
    42481 ttgacggcgt gcaaaaagtg aaagtaaatt tgaaaaaagc aaacggtacg gtcaagtttg
    42541 atgaaacaaa agttcaatcc gaaaagattt gtcaagcaat caatggattg ggttataaag
    42601 cagaggtgat ttgatggcaa cagcagaaac caaaatggat acgttcgtca ttacaggtat
    42661 gacttgtgca aattgttctg ctaggatcga aaaagagctg aacggacaac ctggtgtggt
    42721 caatgccaca gtcaacttgg ccactgaaaa agcaagtgtc aaatatgaag ggacgaccac
    42781 tgaaaaatta atccaaagcg tagaaaacat cggttatggt gcaattttgt acgatgaagc
    42841 ccacaaacaa aaaattgcag aagaaaagca agcgtatttg aaaaaaatgc tattcgatct
    42901 tatattgagt acggttttga ctttgccgtt gatgttgtca atgattgcta tgatgttagg
    42961 aagccatgcg gcgattgttc acttttttca ttttccaatc gtccagttag tgctgtctgc
    43021 tcctgtccaa ttttatgtcg gtgctcgttt ttataaaggt gcgtaccatg caatcaagac
    43081 gaaagcacca aatatggatg tattggtagc aattggaaca tcggctgcct ttgcattgag
    43141 catttacaac ggatttttcc gtggacaccc ccaagatctt tattttgaaa gcagcagtat
    43201 gatcatcacg ctgattttgc tcggcaaata tttggaacat acagcaaaaa cgaaaacagg
    43261 taatgcaatc aaacaattga tgtctcttca aacaaaaacc gcccaagtga taagaaacgg
    43321 gaaagaagaa acgttagcga ttgaagaagt agtcgttggt gatcaactag tcatccgtcc
    43381 tggtgagcag attccagcag atggtcgaat tatttcagga agcagtgcga tagatgaaag
    43441 tatgcttact ggtgaaaatc ttcctgtaga aaagaatccg gatgacacgc tttttggcgg
    43501 gacaatcaat acgaatggtc tgctacacat ggaagtcaca caagtcggca agcagacagt
    43561 attagcgcaa atcattcaaa tggtggaaga tgctcaaggg agcaaagccc caatccaaaa
    43621 aatcgcagat cggatctcag ggatttttgt tccgatcgta ttagtcatcg cgtttatcac
    43681 gctcatagct acaggtttga tcactggtga ttggcaactc gcattgatcc atagtgtctc
    43741 tgtattagtc attgcgtgtc catgtgcatt aggtttagcg accccaacag ctattatggt
    43801 aggaacgggt gttggtgcac gaaatggaat tttaattaaa ggcggagaag cattagaagc
    43861 agctgctcac ttagacagca ttgttttgga taaaactgga acgattacag aaggaaagcc
    43921 caaagttaca gatctagtcg gttcaaaaga agtcctatct attttttata ctttagaaca
    43981 agcttctgaa catccactag gtaaagcaat cgtggaatac ggaaaactgc aagaagcagc
    44041 aacatacgat atgattgatt ttacagccca cccaggtgct gggatcagcg gtacgattaa
    44101 tggagtacgc tattttgcgg gtacacgtaa acgattaatt gaattgaacc tatcatttga
    44161 tgaataccaa gaacacgcgt tagaattaga acagcaggga aaaacagtca tgtttttagc
    44221 tgatgaaaag caagtgatcg ggtggattgc cgtggctgat caaatcaaac tagaagccaa
    44281 acaagcaatc aagcagctcc agaataaagg attagatgtt tttatgctga caggagacaa
    44341 caaactggca gccgaaacga ttggtaaaca agttggtatt gatccaaagc atatctttgc
    44401 tgaagtactt ccagaggata aagcagccta cgtagaaaaa cttcagaaag atggcaaaaa
    44461 agtcggtatg gcaggcgatg gaatcaatga tgcaccggcc ttagcattag ctgatgtcgg
    44521 tatggcaatg ggaagtggaa cggacattgc gatggaaaca gccgatgtga cgctgatgaa
    44581 tagcagtttg gcatccattg cacaaacaat cgagttgtct aggttgacat tgagaaaaat
    44641 caaacaaaat cttttctggg cttttgtcta taatacgatt ggtattcctt ttgccgcact
    44701 aggctttttg aatccaatca ttgcaggagg agcgatggcg tttagttccg tcagtgtatt
    44761 gttgaactcc ctgagcttaa atcgacacat gaaaaagtaa gagaagggat ggagtaaaaa
    44821 tgaatgggaa cgatatggag aaaaaacatg agcataaaaa agaagtagta aacaaagaaa
    44881 gtacagaatc tcatgaaaaa cagatggaga tgaaacatga ccatagtcag atggatcatt
    44941 ccatgcacat gggacataac catggagata tagcaaacag caaacaaatg gaccattcga
    45001 tgcacatgga tcacgaacac ggcggcatgg atcattccat gcacatgggg aatttcaaac
    45061 aaaaattttg gctatcgctt atcctagcta tcccaatcat tcttttttca ccaatgatgg
    45121 gaatggaatt tccatttcaa gtaacgtttc ctggttctga ttggctcgta ttgatcttag
    45181 caactatact atttatctat ggcggtcagc catttttaag tggagctaag atggaattga
    45241 aacaaaaaag tcctgctatg atgacactga tcgcgatggg gattacagtt gcatatattt
    45301 atagtgtcta ttcgtttatc gcaaatctgc tcaatccaca tacacacgtc atggatttct
    45361 tctgggaatt ggctactttg atcgttatta tgctactagg tcactggatt gaaatgaatg
    45421 ctgtgtcgaa tgccagcaat gctttacaaa aactagcaga attactgcca gaatcagtga
    45481 aacgcttgac gaaagatgga aaagaagaga cagtttcatt gaaagaagta aacgaaggtg
    45541 atcgtttgat cgtccggtct ggtgataaaa tgccaacaga tggtgtaatc ttgaaaggtg
    45601 aaacaattgt tgacgaatca gctgtgactg gcgaatcaag aggaatcaaa aaacaagcca
    45661 atgacaaagt gattggcggt tctatcaatg gggatggaac gatcgaaatc gaagtaactg
    45721 gaactggtga aaatggttat ctagcgaagg ttatggaaat ggtgcgaaaa gcacaaggag
    45781 aaaaatccaa gctagagtca ttatctgaca aagtggcaaa atggctgttt tatgtcgcat
    45841 taattgtggg tatcctggca ttcatcgcct ggttgttctt gacagatctt cctaatgcat
    45901 tagaacgtat ggtaacagtc tttatcatcg cctgtcctca tgctctaggt ttagctatcc
    45961 cattagttgt tgctcgctcc acatcaatag cagctaaaaa cggattactt ttgaaaaacc
    46021 gcaatgcact ggagcaagcc aatgatctag atgtgatcat gctggacaaa acaggaacac
    46081 taacagaagg aaaatttact gtaacgggtg tcgaagtact tgatgatgcg ttcaataaaa
    46141 atgaaatcct tcaatatcta ggagccttgg aagcgaatgc caatcatcca ttggctgttg
    46201 gcattatgaa ttatttgaaa gaacatgaaa tcaagcctta tcaagctgaa aatctaaaaa
    46261 atctttcagg agtcgggcta gaggcaacag tcaaaaaaca acaagtgaaa atagttaatg
    46321 aaaaagaagt tgagcgtctc ggattaaacg tagaacaagc attattgaaa ccttaccaag
    46381 aacaaggaaa taccatcagc ttcctgattt tagaaaatca tctagctgca atcgttgctt
    46441 taggtgacgt agtaaaaaca gaagcaaaag aatttatccg aacattgaaa gaaagaaaaa
    46501 tcactcctgt catgcttact ggtgacaata aaaacgctgc ccaagcagta gcagattatt
    46561 tagggatcga agaatattat ggcggtttat taccagatga taaagaagct gttgtccaga
    46621 aataccttga ccaagggaaa aaagtaatca tggtgggtga tgggatcaat gatgcgccaa
    46681 gtttagcccg tgcttcaatc gggatggcaa tcggtgcagg gacagatatc gcaatcgatt
    46741 ctgcggatgt cgtattgaca aacagcgatc caaaagatat tttgcatttt cttgatctag
    46801 ccaagcaaac gcgaaagaaa atgatccaga acctctggtg gggggcaggt tacaatattc
    46861 ttgcaattcc gctagcagcc ggaattcttg cgccaattgg tatcatatta agtccagcag
    46921 ttggtgctgt cttgatgtcg ttaagtacgg tagttgtagc tctaaatgct ttgacattga
    46981 aaatcaaata ataatgaaaa aaggttgtga caaaaaacgt gtcacaacct ttttttcgag
    47041 taatcgatac tcaatcagct ggttctcggc attccagaag tagattctcc agaaatttta
    47101 tcttattttt tgaagcggct cccttttgtc agaatttttt tgtataccgt ttttcttatt
    47161 atacgcgtta tttctaaata ggttttttta tcgaatttta ccggatctgc ttgtcgtcca
    47221 tatatttctg cttcatattg agtagttaat cgaatgaaac gcagatctag cttgaattct
    47281 tgttctattc gcaggctata ttcctgcaaa gattctgcgg gtttgcgcgg taccaatgtt
    47341 tccaaagggt taagaagttt ttgatatgca atcgtattag tagaagcaaa aaaatgaaga
    47401 tagataagat aacttgtcca gcgcagagat tttctgaata acaatagaaa gaacaacaag
    47461 acggataaac acactccaag gctgaataag acgattttat aagctggtgt gagtgttgtt
    47521 tcttctgatg gaatgacctc agatctctca ctggaaatag tttctgccat attttcgctt
    47581 tcatttggtt gttcgatagc aggctggacg gtttcggttg tttcagcaac tggttgtgaa
    47641 cccattggat tggcaaatga aggggttggt tcaaatggaa gccagccaaa tccaggaaag
    47701 tatacctctg gccaagaatg agcatgacta ttgagaatgc tgtattcctc attcccttga
    47761 ttatctgtgc ctgttctgag cccatttgta aaaccttttg tccagcgagc tgggatacca
    47821 agtgttcgaa gaaggacgac catagaagtt gaaaagttgt cacaatagcc gacttttgaa
    47881 tcaaagagga aataatccac ataatcgtgt tctttcggag tatgcccggt atccgttttg
    47941 gaatagcgga aatttccgct tgtttttaaa tactgttaga cagcttcgac tttacgaatt
    48001 aaagttgctt catttttggt caattcgacc gccaagtcct caatacgttt aggcaaactt
    48061 tttggcaatt gaagatttgt ctgctgattt tctaaatcat cagcttttaa ctgactgctt
    48121 tttaattctt ctctcgtaaa aactaacctt tctgtttgta aagttgcaga acgtgtcgat
    48181 tcattcggga caggaatgaa tcgctggctt tcaggcaaat agtaaagcgt ttgtccacta
    48241 gctaatgaaa aatcagtcaa ggtagtattt ccataaggat aaggtaagaa agcttgtttt
    48301 tttagggtca atgtaatgtt ttctttgtct tctataacgg tttggtagct cgtgatgtca
    48361 acagggaatt cttggattgg ttggatgggt gcgtcctgat cttctatcca tccagaaccg
    48421 gtgtaatgat ttttattttc gatcttccaa tagccatttc ctttttggtt cgctgtaaaa
    48481 acaacttcgc cattatcata taaaggtcca ccaagctgtt catcattctc actgaagcct
    48541 gttcttgcac ttccggttaa ttgatactca tggagtgtgt cataaagccc ttcatcatta
    48601 agatattctc ggatcggttc actcgtttcc tcgagagatt ggtgtacttt cggaagatat
    48661 aaaggcaaga tagcgctaaa agttaagaaa caaataagca gaactgtagt caatgactgg
    48721 aatcgagtgt ttcccgacaa caaccggatg gagaaaaatt gccttgctgc cagatacacg
    48781 aacaaaacag ttactaccgc aatcatttcg tacagtaaat cttttttccg aaaaacggta
    48841 agcatcatga agtaagccat caaagaaaga aagggtgcgt accaatagtc gtaatccacg
    48901 gtcaatgtaa taaataaata gatcagcaac attatgagaa aaaaagctgt cttttctggc
    48961 aaataataaa gtttaccttg ggatagatgg aagaattcct ctgtaactga cgaaaaaaac
    49021 tgtaagatcc ataatatgga aaatgtcttc gaaggaaaat ataaaaacaa acaacctatc
    49081 attactggca aagaaagcag caaacgtacc cattttttag gaatacagca gacaataagg
    49141 cataggacac caatagttag gataagaagg tagctgtttt gcaattgatt agctactaca
    49201 aatatcggag cacctacact tagtatcaca aaggagtaaa cgccacgcag ccatttttcc
    49261 cgtttaatcg tcaatccatt cacctcctgg caatgaacat atcttgtctt tttcttggac
    49321 catgagttgt ttttctacga aataaacaag ataagccgtt cgatcttttg acaatgtttc
    49381 aatagcttct gagacttcag gagaattctc aggaacaaag aggacgattt gtttttttgc
    49441 atattttttc aagtgtgcga aaaaagcttt ctcatcgcca gcttccaaag aagcaaaata
    49501 ggtatgaccg gggtctttgc catgaaaggc gcggttgcct aatatcagaa gttccggctg
    49561 cttttctttt ctcaaatgcc ataaagaaaa atacaagtct agcagaggtt caaattttgg
    49621 actgggcgaa ccccaaaagc aaaaaagagg agaacattct tcttcttgat aatcgtattc
    49681 acggtataac cactcttgag tatgagcaga caatttccaa tcgattcggt tgaaagaatc
    49741 acctggacga taagcgcgat gttcccgaaa atcaagacct ctttggtacg cgttcagctg
    49801 ctgttttttt tccagaactc tctgtaattt ttctgcttga atcttacaaa aagcaggcag
    49861 aacagtcaca ggaaaagaga tctctctttt tgcacttttt ctgaaaagtc ctagactatc
    49921 ataggctgta aaaatgacag ttaatgtttc atggcatcct ctttcttttg gctgccaaag
    49981 caattggact tcttgcggtt tatttgtaag aatcaaaatc tcttgttttt ttctgcaaag
    50041 attgctggga agccgatttg taaacgaggg agaggtaaag aaaaagttct cttttttgct
    50101 atggaaaaaa taacctgcat actttttttg cgataaacga gtggctgcaa tgtacaagta
    50161 accgaaagtt tctttagaaa gctgcttaat gaaatcaagc tccatgatac ataaagagta
    50221 aaaaaggcca acaagaacca gcctaaagaa ttgttgaata caagagcaaa aagcacaatg
    50281 atgaaatata ccagaaataa cacaaaatta atgaaataat ctttgatttt ttccattgtc
    50341 tacctcaatc tacctcacag gaataggaat ttgtgcaaca agagtatgaa tgatttgttt
    50401 cttttcgtct tccttcaatg aacgatcgta aaaaatcagg cgatgggaaa acacgtaagg
    50461 aactaaatcc tgcaaatctt tgggcgtgac gtaatttctc ccatggatta aagcataagc
    50521 ttttgctgcc tgaataaagg aaaggcttcc tcgtggactg atgccaagct ggatggcagc
    50581 atgacttctt gttgctgaca ctaactgcaa ggcataagat gccacttcgg gactgacaaa
    50641 gacattcgcc acatttgttt ttatttcttc tatttcataa ctgttcaata cttcatttaa
    50701 gttatccaac actttttcga atttatccaa aagtagcaaa agttcgtcat caaaagaagg
    50761 ataaccgatt tgtagtcgga ataaaaaacg atcgagctgt gcttccggca aaggatatgt
    50821 tccttcgtac tcgattggat tttgcgtagc taagacaaaa aaatgattgt ctagagggta
    50881 tgtattgttg tcgatcgttg catgattctc ggccattgct tctaaaagtg ctgactgagt
    50941 acgaggagtc gtacgattga tctcatctgc caacaagatc gtggtaaaga tcggtcccgg
    51001 acgaaattca aactcttttg attgtgaatt gtatacggaa aaaccaagaa tatcactagg
    51061 cagcagatca ggagtacatt ggactctgct gaaggttccc tgtacagctt ttgctagtgc
    51121 tttgaccagt aacgttttac ctacaccggg aatatcttcg aataagacat gaccacctgc
    51181 taatagggcc acgatcatca attggatagt tgaacgtttg ccgatgaccg ctttttctaa
    51241 ttcttatatc aactgatcga ttttttgttt ggattcttct attttttctt ccattttcac
    51301 atcaccttct cataaaatcg ctatttattt cattgtaatg gaagggaata catcctacaa
    51361 gcaaagtgat cttattatgt ttttttaaag tacattcgtt ttgaaatgag aatacgatta
    51421 aattgagtaa aataaaaaat aatgaggaag atttgcgtat ttaaataaat agaaagggag
    51481 ttttttatct ttttaggagt gaactcattt tgatcaaaca atatgtttag aaatcaggtg
    51541 agaaatgatc gatgagatgg caaaaactat tatcaaaaaa tgcacagaat ttattttcac
    51601 taatcaatct aatgaaagat ttgatctggc gaaaagctga tttagctgaa gaactgaaaa
    51661 tcgatgttaa aacagtggat cggcatctga aacgtttggc ggaaagcgac tgtcccattc
    51721 agctgattct tcataagaaa accgttcagt tagtttatga tcaaggatat agcgaacctt
    51781 atctcttgtt tgtttttctg atccaatcac ccccgttttg tttactgaaa aatctgctat
    51841 taaatgaacc ttacgagcag aagtacgatc gttctacgca agagcgattg agaaagtggt
    51901 ttggcgagta ccacctttct ttttcttata aaaaagcaca gatctttggt tcagaaagaa
    51961 aagtaagata tctgctgttt tactttttga aagattttcc gcattttttt gcagatgatg
    52021 gagagcagtc cttttctgag atgttcatcc aattattgaa gcaaagaaag gatccggcag
    52081 aaatcgagat tttaggagac agtcgaacac tattctttga attgtttcct gaattgctat
    52141 ttagagagga gcagattttg ccaataaaag aaaaaaatta tctagcaatt ttgcgcttga
    52201 gctgtaacca gattactgaa aagcggtgga aacggataga agagacacaa gactatcgga
    52261 aattgacgat tgtgactgaa aagcttgatt cgctgttttg gctgaatagc gaatttgaga
    52321 gcgaaagcaa atcaaagctg ataaaagtac tttaccaaga atggcttcgt tacgtaattg
    52381 gtatccatga ccggctgata gaagaagttc gttattatca ggaaacatta gaaaaatttc
    52441 ccaattccaa agataaacta tatcaatttc ggctagcgat ccaacaagca cttgcacacc
    52501 ccgcagtaga gtgggggatg cttttactga aaacactcca ggaacgaggg tatttagatg
    52561 tccaccctcc tgaagtgaaa atcgtctttt tttttcgcta tgaacaccaa gaagcaagaa
    52621 gacttgctgg ggtactgaac aaaacgctaa gacatcgaaa acggatctct atacatgtat
    52681 gtactcgaaa taagccgcct cggtatgcag atctagtgat caccgatcat ttttttccac
    52741 tagaccataa cgaagatcaa aaaacgtatc tgtaccatcc ttcatttggt atcgaaagac
    52801 ttcttaccga tatcaattac tggttaggca aaaaccgata aaacctttgg aaaaaagatc
    52861 aaaacaagtc agtctgtgct ataataaaaa gcgattagta aaggaggaaa tccattggaa
    52921 ttaaagaaat tagaagttcc cacatcttcg atcacagaag tgaaacgatc gccgatggat
    52981 gtatttgctc aagcgcgaga cgcgggaact ggcgtatata tatttaatcg tgaaaaagta
    53041 gctggcgtga tgctgaccca agaacaatac aaaacgctct tgcaagaact agatgtcctt
    53101 cgtcaaacag tgaaagtgga gaggaaagaa ccaaaagaaa taaacgaaga acctattaac
    53161 aaaacagctt ccgtcgtatc caatgctgaa gggatggaag agctggccca aacattacag
    53221 cactcgctca ttactggtgt gacgattaca gcaaaaaatc tagatgaacg aatggtagca
    53281 ctaggtttta ttacgaaaaa gaccggattt ggcggagtcg tggatatgct tagcgagttg
    53341 atggaaactg ggaaaatcaa ctaccagtta agaagaaaac cgcaaaatcg gacaatcata
    53401 gctgaaatca taggagagca aaacagtcaa tctcaattga tggataaaat catcatcaag
    53461 aaaatttttc tacatgagct ataagaaaac catgttcgca cttctaggca agccaggctt
    53521 tgaagacctt gccaaagaat tgaacgaacg cctttaagat aagggttttt cgctagattt
    53581 aaaaaatcag tgatagaaac atgttcgctt ataaataaaa ttagtgatag ttttagtgat
    53641 agtgagctta aaaaagcact caatttgagt gcttttttat ttgctcttct atatagtttt
    53701 ctagttgatt cattgatctt tttttcactt cgggagaaat atgggcatag acttgtgtag
    53761 tggaaacatc tttatgacct aaaaaatctt tgatatcttc taaaggtatt cctgcttgtc
    53821 tcgttctagc tgaaaaagca aaacataatg ttggtgtttt cctttcagca tatctatcag
    53881 caagatgtag agtaggacaa cctcgtgagc caaaagtcct gtgcaaatcg agttacaaca
    53941 cacacatgat aaagaaactt ttgaaaagaa ctataaagta tacgttgaat tggcaagaga
    54001 ttcagcaatg aaatatggta ttccattaac attagacact ccttataacc aaccaggaat
    54061 caaatcgcat ttatgggtaa cacaaaatat ttggggcgat catacagatc cttacggtta
    54121 tctttctgaa atgggtgtaa gtaaagaaaa actagcctat gatttggctc atggttttac
    54181 ggatgataat ccaactactt cggaggataa accagtaatt gatccaactc gagcaggtgc
    54241 tgcaaattct acgctgacag atggaacaaa ttacgcccac attgatcagt tcggagaaat
    54301 cgaaaacgca aacttgcatg tagctggatg gcacattgct aattataaat acgagtatat
    54361 tttcattatg gactacaata ctggaaaaga actagctaga gtaagagctg atgggattta
    54421 tagaccagac gtaaatcaag cttataatac tttaggaaat gttggctatc atgtatcttt
    54481 caatatgcgt gatttcccta ataagaaagt atacgtcatg atgcgtgcga caaacgatcc
    54541 agaaggaaat actaagggtg gagcacaaga ttttcatgac aagcgttggt atttaaatat
    54601 tccacaacga taaaaaatcc ccccctcttt
//
LOCUS       DANJMI010000018        50965 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000018 DANJMI010000000
VERSION     DANJMI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.018"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(56..1561)
                     /locus_tag="PWU81_001241"
     CDS             complement(56..1561)
                     /locus_tag="PWU81_001241"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDL1109955.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1653..2243)
                     /locus_tag="PWU81_001242"
     CDS             complement(1653..2243)
                     /locus_tag="PWU81_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL1109956.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2422..4068
                     /locus_tag="PWU81_001243"
     CDS             2422..4068
                     /locus_tag="PWU81_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL1109957.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4117..4836
                     /locus_tag="PWU81_001244"
     CDS             4117..4836
                     /locus_tag="PWU81_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL1109958.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5315..6262
                     /locus_tag="PWU81_001245"
     CDS             5315..6262
                     /locus_tag="PWU81_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1109959.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            6274..7194
                     /locus_tag="PWU81_001246"
     CDS             6274..7194
                     /locus_tag="PWU81_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1109960.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            7235..8692
                     /locus_tag="PWU81_001247"
     CDS             7235..8692
                     /locus_tag="PWU81_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1109961.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            9037..9651
                     /locus_tag="PWU81_001248"
     CDS             9037..9651
                     /locus_tag="PWU81_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL1109962.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            9644..11368
                     /locus_tag="PWU81_001249"
     CDS             9644..11368
                     /locus_tag="PWU81_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL1109963.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            11365..12816
                     /locus_tag="PWU81_001250"
     CDS             11365..12816
                     /locus_tag="PWU81_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1109964.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(13289..15442)
                     /locus_tag="PWU81_001251"
     CDS             complement(13289..15442)
                     /locus_tag="PWU81_001251"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HDL1109965.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(15444..16490)
                     /locus_tag="PWU81_001252"
     CDS             complement(15444..16490)
                     /locus_tag="PWU81_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1109966.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            16640..17929
                     /locus_tag="PWU81_001253"
     CDS             16640..17929
                     /locus_tag="PWU81_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HDL1109967.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            17942..20644
                     /locus_tag="PWU81_001254"
     CDS             17942..20644
                     /locus_tag="PWU81_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL1109968.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            20629..21486
                     /locus_tag="PWU81_001255"
     CDS             20629..21486
                     /locus_tag="PWU81_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL1109969.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(21557..22456)
                     /locus_tag="PWU81_001256"
     CDS             complement(21557..22456)
                     /locus_tag="PWU81_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL1109970.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            22625..24796
                     /locus_tag="PWU81_001257"
     CDS             22625..24796
                     /locus_tag="PWU81_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL1109971.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            24864..25205
                     /locus_tag="PWU81_001258"
     CDS             24864..25205
                     /locus_tag="PWU81_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL1109972.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(25481..27235)
                     /locus_tag="PWU81_001259"
     CDS             complement(25481..27235)
                     /locus_tag="PWU81_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1109973.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(27246..28082)
                     /locus_tag="PWU81_001260"
     CDS             complement(27246..28082)
                     /locus_tag="PWU81_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL1109974.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(28084..28767)
                     /locus_tag="PWU81_001261"
     CDS             complement(28084..28767)
                     /locus_tag="PWU81_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HDL1109975.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            complement(28926..30320)
                     /gene="uxaC"
                     /locus_tag="PWU81_001262"
     CDS             complement(28926..30320)
                     /gene="uxaC"
                     /locus_tag="PWU81_001262"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL1109976.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            complement(30359..31858)
                     /locus_tag="PWU81_001263"
     CDS             complement(30359..31858)
                     /locus_tag="PWU81_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDL1109977.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            complement(31851..33272)
                     /locus_tag="PWU81_001264"
     CDS             complement(31851..33272)
                     /locus_tag="PWU81_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDL1109978.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            complement(33332..34324)
                     /locus_tag="PWU81_001265"
     CDS             complement(33332..34324)
                     /locus_tag="PWU81_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1109979.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            34556..36754
                     /locus_tag="PWU81_001266"
     CDS             34556..36754
                     /locus_tag="PWU81_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1109980.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(37212..38084)
                     /locus_tag="PWU81_001267"
     CDS             complement(37212..38084)
                     /locus_tag="PWU81_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1109981.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            38384..39358
                     /locus_tag="PWU81_001268"
     CDS             38384..39358
                     /locus_tag="PWU81_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1109982.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            39380..40072
                     /locus_tag="PWU81_001269"
     CDS             39380..40072
                     /locus_tag="PWU81_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL1109983.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            40038..41546
                     /locus_tag="PWU81_001270"
     CDS             40038..41546
                     /locus_tag="PWU81_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL1109984.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            41620..42723
                     /locus_tag="PWU81_001271"
     CDS             41620..42723
                     /locus_tag="PWU81_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL1109985.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            42726..44063
                     /locus_tag="PWU81_001272"
     CDS             42726..44063
                     /locus_tag="PWU81_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL1109986.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            44050..44928
                     /locus_tag="PWU81_001273"
     CDS             44050..44928
                     /locus_tag="PWU81_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDL1109987.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            44925..45848
                     /locus_tag="PWU81_001274"
     CDS             44925..45848
                     /locus_tag="PWU81_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HDL1109988.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            45845..46255
                     /locus_tag="PWU81_001275"
     CDS             45845..46255
                     /locus_tag="PWU81_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL1109989.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            46259..47827
                     /locus_tag="PWU81_001276"
     CDS             46259..47827
                     /locus_tag="PWU81_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL1109990.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            48067..49485
                     /locus_tag="PWU81_001277"
     CDS             48067..49485
                     /locus_tag="PWU81_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1109991.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            49497..50807
                     /locus_tag="PWU81_001278"
     CDS             49497..50807
                     /locus_tag="PWU81_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL1109992.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT        16394 a         9009 c        10414 g        15148 t
ORIGIN      
        1 aaatcacagt cccttttttt cttagaaatg ggaaaccact agtatataaa taccattatt
       61 cttcaatcaa ccgcttcacg attgcataat ccccttcata aggttcatct acaccattga
      121 ttttttgata gggatccacg ccttttcccg caataacgac tgaatcttgc ggagcgcttt
      181 ctgctaaagc gcgtcgaatc gcttcaggac gatccatttc ataatgaacg atcaaatctg
      241 gattagtaat tgcttcattg atttcttccg cgattttttt cggatcttca aatgctgggt
      301 catctgccgt caaaaaggcg atgtcagctg tttctgatag cacttttcca aaatcatgtc
      361 gacgggagat tgctttgttt cctgggctgc ctaatacaac aattacacgg ccatctggat
      421 gttcattttt ggcaaattcc agcaatgttt tcaagcttag ataattatgt gcatagtcca
      481 catagacatg cgctccattg gattggatca attgatccat tcggcctggt acacgagctt
      541 cttttaatcc gtcttgtgca tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg
      601 cactggctgc attttcttgg ttgaatcctc cagccagcaa aatatcataa gcagtatcgc
      661 agatagctaa gcgatcttca tgagaagata aagtaaaacc gtgagtacct ttatctgaac
      721 ggatcacttg ataattgctt tcttccgaac gaccataagt gatcgttgga atctggaata
      781 gatcggctgt ttccctaagt aaatcaaaat agtcactttc atgatttaaa acaactgttt
      841 ttgaattcaa gatcaattgt cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga
      901 tcggactgat atggtcaggc gagatattca agaagattcc gacatctaaa gtcaagccgt
      961 atacgcgttg tgttttgtaa gcttgtgaag aaacttccat cactagatga gtcataccat
     1021 tttcaactgc ttctgacatc atacgatata aatctaatga ctcaggcgtt gtcaaatggg
     1081 atttgaaata tgttttgcca tctaacgttg tattcatagt agaaagcaat gctgtttttt
     1141 tatttgttgt gtgatccaag attgcttttg taaagtacgc agcagttgtt ttaccttttg
     1201 ttccagtgaa tccgatcact ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa
     1261 gaagcgccat tgctttacgg atatccgtta cgatgattcc tactgctgaa ttttcgtatg
     1321 gttgttctga tacataatat tgaataccct gttcgattgc tgaatccaaa tattcttctt
     1381 tgaaattcat tcctttacag aaaaacaaag tagaagcatc agcttttctt gaatcataac
     1441 ttaactgaag aaattcagct ttttctggta catcaagatg ccagcctctt gttgaaacaa
     1501 attcttttaa tagattttct tttaatagta aatgacggat gtcttctaaa gagaagctca
     1561 ttgattgatc cctcgcttta ttcaatttct tggtttatta tagcacactc tttttaatat
     1621 atcgtttgtt tccgctatga caaaatccag ctttattcaa attttcttga cacaggggac
     1681 aagcggatga gtattttcat aaacaatgaa ctgactgtaa tagataggag aacgaatagt
     1741 gtctccaaca atccaaaaac ataataattc aaaaagacga taagactatt ggtaagtaaa
     1801 agtccttgcg gcattggaag ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata
     1861 ccgccatttg aggccccgac tgtgtacaat gtgtaaaccc caagactgaa caaaagactt
     1921 cctgaaatca gtgaaaagaa atgtgaggag atcatttcca cttttggaac aatctctaaa
     1981 acaacaggaa aaagcagact tcccgctaca gtcttaaaaa atactgattt tcctaatagg
     2041 acagcggcta gaagcagcat cataagattg atgctccaaa cagtaactga gcgttggata
     2101 gaaaaggccg tttcaactaa atgtccgatg gcgcccactc cggcggaagc tatatcgtga
     2161 gggagcaaga accaattcaa tccaactgtg ataaagaaaa gacctaaaaa tataacgggt
     2221 agttttttta tttcttgtgt cattgatatc tcctataaat gttagccata aaaggctgta
     2281 tatttcttta ctttaccaaa aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa
     2341 agttatataa aatcgattgc cagtttctgt tttaccaatt aaaatgatag taatgttttg
     2401 tagaaagttg gaatctgacc gatggataat caaagaaaac cagcattaga tcaattaaca
     2461 ccgcaagaaa aaatggcacg aggctctgct tggatgaccg ccagtaatat catttcaaga
     2521 ttgctaggcg ctgtctatat cattccctgg tatgcttgga tgggcgaaaa tgctaaggct
     2581 gccaatggat tattcaatat gggatacaat atatatgcat tgtttctact gatctctaca
     2641 gcaggaatac ctgctgcgat tgcaaaacaa actgcacgct ataattcatt gaatgaatat
     2701 ggcacttcac gacgattatt tatccgtgca ttacagatga tgggcgggct aggcctgttg
     2761 tttgctttgt tcatgtacat cgcctctcct tggctagctc atgcttctgg tggcggagaa
     2821 gaactgattc caacgatgcg ttctttgagt atcgctgtat tggtttttcc ttgtatgagt
     2881 gtgatacgag ggtattttca aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt
     2941 gtagaacagg tggcacgtat tttttatatg ctattggcta cattcatcat catgaaagtc
     3001 atggatggtg actatgtgac ggcggtcacg caatcaactt tcgcggcatt tatcggtatg
     3061 ctagcaagta ttcttgtcct tttgtatttt ttgaagaaac atcaagcgta tacatcggcg
     3121 tttattcact acagcgaaaa caaaataaat attgcaacga aggaattgtt gttagatacc
     3181 gttaaagaag cgattccgtt tattattgtg ggttcagggg taacgatctt caaattagtc
     3241 gatcagttca cttttatgaa gatcatgtct gcatccaccg agtattccaa tgcacagtta
     3301 ctggatcttt tttcgatttt tagtgccaat ccagataagt tgacgatggt agttattgca
     3361 ttggcaacat cgatcgcgtc tactgggtta ccgttgatta cagaagctgt tacgttgaaa
     3421 gatcgagtag gtctggcaaa attgacaagc ggaaatttac agttgttttc attttttatg
     3481 tttccggcga catttggtgt tatgttactt gcctatccgc taaatacatt attttataca
     3541 ccggattctc taggaagtaa tgtactgatc caagcaagtt ttgtaggact cttcctagga
     3601 ctatatatgc tagtttccaa catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat
     3661 tatttggtta ttgggtttct agtgaaactc cttcttcaat acccagccat tcgtttattt
     3721 gaagtatacg gccctttatt ggcgacaatg atcggatttg ctgtgtcttg taccttgatc
     3781 ttaaagagaa tccatgaagt agcccgcttt aaaccaagtt ttgtctggcg caggacattg
     3841 ctgatcttta ttcttacctt gattatgctt ttggcttcgt ggatcacaaa gatgattttt
     3901 ggtgcttttt taaacgaaga tagcaaattc cagtctctat tgttgattct tgtagtagca
     3961 ggtatagggg ctttagtgta tggttattta gctttgaaaa tccgcttggc agaaagacta
     4021 ctaggaccag gaatagcgcg tttacgacat aaattaaaaa taaaataagt gaatcagaag
     4081 agctagattg atgctctttt gaaatggggg aaacgcatgc gtttagataa gtttttagca
     4141 gatatgggac ttggcagccg caaagaagtc aaaggattga tcaaaaaagg aacgatcgaa
     4201 gtcaatggac aagtggtgaa aagcgataaa gcgcaagtaa atgaattcga agatgcggtt
     4261 acttatttag gagaaatcat tacgtatcaa aaagattttt actacatttt gcataaacct
     4321 gcaggagtca tttctgctac tcaggataat catgatcaaa ccgtaatcga tttattggaa
     4381 gaccaggatt atcgggaaga tctttttcca gtcggccgat tagataaaga cacagagggc
     4441 ctcttgattt taacgaatga cggggtgttt gcccatcagt tactatcacc taaaaaacat
     4501 gtggagaaag aatatctagc tgaggttagc ggtataatga cagaagaaga tatccgttta
     4561 tttgcagaag gactgattat tgatggcgat gagcagacat tgccggcaaa actccaaatt
     4621 gatcaagtag atcaagagac agaaacttca attatccgtc tgattttgca tgaaggaaag
     4681 tttcaccaag ttaagcgaat ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga
     4741 atccgtatgg gaaaattttg gctgcctaaa gatttgaata aagggcaata tcgacccatg
     4801 accaaagaag agctagaaca agtgagagaa aaataattga atggatagct aaaagaataa
     4861 gcagagtagt tgtagcgcca tctgaaaagc agacttgtcc gtctgacttt cggatggctt
     4921 ttttgtgaaa tttcggctta tttccgtaga agctacttct ggaacaccgt ttgttcggaa
     4981 taagggactg tgacaagact tttgttacag tccccattgt tccttgtgac tcagagttag
     5041 atagtaagga cacgatctcc ctaatactca ggccaaaacg ctgaattcac aacctctgat
     5101 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
     5161 ctattttccc gtttttttct ctaatactca ggccaaaacg ctgatttcac aacctctcct
     5221 atactttgtt tcattttccc tatactttgt ttatttattc tcattattga aagcgctata
     5281 attacgttgc gaatgaatcg aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca
     5341 tttttttatg aatctatggc gttataaagc acttgtcctg atggctgtac cgggtatgct
     5401 ttggatgatt tttttctttt acatacctgt aatggcgaat gtcgtagcat tcaaaaattt
     5461 tcatatttca gcagatgggt tcttggctag cttgagggaa agtccttggg tgggttggga
     5521 aaatttcaaa tttttatttt cttcggatca agcatttctc atcactaaaa atacaatttt
     5581 atataatgta gcatttatcc ttttgaatct gctgatctca gtcgtatttg caatcattat
     5641 gagcgagttg agaaataaac gactcgttaa agtttatcaa acgatgtcct tactacctta
     5701 ttttctatct tgggtcatta tcagttactt tgtttacgct tttttaagtc ctgacaaagg
     5761 aatattcaat cagtggatca ctggacatgg aggagaagct gtcaattggt ataatgaacc
     5821 gaaatattgg ccgtttatct tggtgttcat tggtacatgg aaagggattg gttataacag
     5881 cattatttac tttgcatctg ttatgggaat cgatccgact tattatgaag ctgctatggt
     5941 tgatggagct agcaaatggc agcagatcaa acatgtgacg ataccacaac ttattccact
     6001 aatgacgatt ttaacgattt tagccgtagg aaacattttc cgagctgatt ttggcctgtt
     6061 ttataacatt cctaggaatt ccggtgcatt atatgatgtg actcaagttt tggatacgta
     6121 tatctacaat ggcctgacat ccacaggaga ttttggtatg acggctgctg caggacttta
     6181 ccaatcagtt gtcggctttg tcttgttgat gatcacaaat acgatcgccc gacgttttga
     6241 tagcgattct gcattattct aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc
     6301 caagtccgct ctttcaataa gacggctaat tttttctcta atataatgat tgcactcttt
     6361 gctatttcct gtattttgcc atttatattt gttatcgcca tctcttttac cgatgagtcc
     6421 atattgtcga ctcacgggta cagtttttgg ccagaaaagt tcagtatatt tggttatacg
     6481 tacttgttcg accagatgca ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt
     6541 actgttttag gtacactgat caatagcacg gctacttctt tatatgctta tgcgatttca
     6601 cggtcaaact ttccattccg tcgttttttt accgtttttt gtttaattac gatgttgttc
     6661 tcaccaggga tggtggccaa ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg
     6721 atttgggcat tgatcttgcc aatggctgtc agtcccttca atattattgt gatgcgcaca
     6781 ttctttaaac gttcggtctc tgattcgatt attgaatcgg cacggatcga tggagctagc
     6841 gaactgcgaa tatttgttca gatcgtttta ccgttagctg ttccaggtat tgcgacaatc
     6901 agtttatttg ccgcattggg ttactggaat gactggttca atgctttact atacatccaa
     6961 gatgataagc tagttccact acagtatctt ttgatgaaaa tccaaagtaa tattcaatat
     7021 ctgacacaaa attcaggagc aggaagccaa ttgacaggcg gattagcctc agttccagga
     7081 gaatctgcca gaatggcgat cgtagtcatc tcgacgttgc ctattgcgat cagctatcca
     7141 tttttccaga agcattttgt caaaggtcta acaatcggcg gagtaaaaga ataaagaaac
     7201 aaaaatataa atcataaaat taggaggaag aaaaatgaag ttatggaaga aactagcctt
     7261 tacaggtgtt tcagcgttgt tgttgggaac attagcagca tgcggaggtt ctggttctaa
     7321 agaccaggca gaagctagca attcggacac tttgcagatg tatcaaatcg gagataaacc
     7381 agataatttc gatcaattga tggaagtagc aaacaaacga atcgaaaaag agatcggtgt
     7441 aaaagtcaat atcaattaca ttggctgggg cgattacgaa aagaaaatga acgtcatcat
     7501 ttcttccggg gaaaattatg atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa
     7561 aggagccttt gctgatttga ctgaattagc gcctaagtac gctgaaaaag cttataatga
     7621 tttagatgaa gcatatatta aaggaaatct ggtcaatgga aaattatatg ctttcccagt
     7681 caatggaaat gtctttgctc aacaagtact tacttttaac aaagctttac tagataaata
     7741 cgatctttca attgatggaa tccaatctta cgctgatgca gaaaaagtct tgaaggaatt
     7801 ccatgaaaaa gaaccaaaca cggcagcttt tgccattgga caaggcttca aagtccaagg
     7861 gaactttgat tatcctttag ggaatacgtt accatttgct attgatctgg atggagatga
     7921 ttcgaaaatc atcaaccaat acgataatga aacgtatatc ggactgttac gtacaatgca
     7981 tagctggtac aaacaaggac tcattccaag tgacgctgca acgagcaata aggattatcc
     8041 actagaagga aatacttggc taatgcgtgg agaaacacaa ggaccgtatg attatggcga
     8101 tacaattttg acaaatgcag caaaacaaga acttgtctca aaagcaatta ctgttccact
     8161 aaaatctaca gctcaagcac aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa
     8221 agaaaaatca gtagaacttc tagggctgct taacagtgat ccagaattat tgaatgggct
     8281 tgtttggggg atcgagggag aagcatggga aaaagtcgat ggttcagatg ataaaatcaa
     8341 attgctagat ggttatcagc caaatacaca tatgtctgct tggaatacag gaaacaacaa
     8401 gattctatat acacaagagt ctattacgga tgacatgatt gccaaacgtg atcaatcgat
     8461 tgctgatgca gaaacttcac cgattttagg attcagcttc aacactgaca gtgtcaaaac
     8521 agaattaagt aatatttcta atgtcatgaa tcaatattta gatggattga atacagggac
     8581 tgttgatcca gatgaaacat taccgaaatt gaaagacgct ttggacaagg ctggctatga
     8641 taaggtattg aaagaaatgc aaaaacaata tgatgagttc cgacaagaat aataatgaat
     8701 aaagacaaag gggttgtgac aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt
     8761 tcaaaagcca gtttctcggt attaagctaa atatatgtaa aaatgtggaa agccatattc
     8821 acaaaatttt tcttactact cgaaactaag cggctatttc acaaactctt ggtaaacggt
     8881 gttcaatcag ctaaccctcg gtattcgctc acaaaacgac aaaatatgag gaactatttt
     8941 tccgtttgtt tctctaataa tcaggtcaag gtgctgattg cccaaccttt ttttgtataa
     9001 tgagaaagta taaacttaat gtttggagga gaagatatgt tagggaaggt tttggaagag
     9061 ttatttatcc ggatctgggt cattataaaa ctaaccttgt atttctggat atatacattt
     9121 gccggcggga tcatctttgg attaggtgcg gcatggaaaa cagtcaatga actattttat
     9181 ttgtacggat tcgagtataa agaaatcaca ataaaaagag gctggaatat atataaaaga
     9241 aattttttaa gaggcaatct gctatttagt ttatttttat ctggtactgc tttattgagt
     9301 tacaatttat atttatctgt tcaaatcaaa gggctcattt ttttagctat tgattttatt
     9361 ttactttttg ccttgctctg cctatttgct acttatcaat acagtatgat tttagatagt
     9421 gaatatacga tctctgttcc taatctgcta aagctaagtt ttatttcggt tttttccagc
     9481 ttttcgtctt ttctaaaaat aattattggt agcggtataa ttttaggcgt gacttggcaa
     9541 ttcaaaggat tgatcctgtt tggtgtcatt ggtcttttga cggtttggaa tggtacgatg
     9601 acaacacatt ggcgtgaaga attagataaa cagttggaaa gttatgaata agtttcggat
     9661 tctattttca aaaggaacct tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat
     9721 cggtgtaacg atgactgcta ccgttatttt tagtatccat acttgggaac aaaatcaaaa
     9781 acaggcagaa aacatgacaa gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa
     9841 aacaaccttg agtaggatca tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa
     9901 tgtgacgacc tacttaacaa aacccataga tcagtatctg atgtatgtat acgaacagca
     9961 aaacagtacc gatgaattag tttctttccc aaatcaaatc aaagatctct acgcaaatta
    10021 tgaagaacta agtgcgatat atatcgtgtt gaatcaacta ccggagtatt atgaatcttc
    10081 tagagaaaac aaaggtgggg aaataaaaaa ggggacacca aaattactag acgcctttta
    10141 tatcaagttg cctatcacac aaggaggagc tgagtcagga agtattttta ttggttttca
    10201 aaagaaagaa ctagatatga ttttagaaaa tctaacgtca ttcaatggac tctctttata
    10261 catgatttct ggaacgacaa atcgactata cacattccat gataataaaa tagctaaaga
    10321 aacgtttgaa agacaagaaa tgatcatcac agagagtctg aagaaaaatt cacaattgcc
    10381 gattgacgat ctggaaaaaa acaattttgt tcaacaccaa gaactgtctg atgattatcg
    10441 tatattagct gtactagata aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc
    10501 gctgatcatt ggtatcatta ttttagacaa tatactgctt atctttcttt ataaaacatt
    10561 caaacgatat tcacagcaag tatctatcgt catgcaggcc atggatcaaa cagcagcagg
    10621 gaatttagag acgcggatcg acataacaaa cacggaatat gaactgaagg aactttcaat
    10681 cggtatcaac gaaatgttgg atagcatcaa tcagtttgta gaagacatct acaaattaga
    10741 aatcaaacag caagacgcac atatgcgggc gcttcaagca cagatcaatc cacacttttt
    10801 atataataca ttagaatata tccgaatgta tgcaatcagc gaaggaagtg aagaattagc
    10861 agatgttgtg tatgcgtttt ccgctttgct tcgaaacaat acgaatcaag agaaaacgat
    10921 cactttaaaa gaagaactgg acttttgcga aaaatatgtc tatttgtatc aaatgcgtta
    10981 tcctaatcga gtagcctatc attttatgat cgatcctgat ttggaaaaga tcgaagtacc
    11041 taaatttgtg atccagccat tagttgaaaa ttattttaaa cacggtattg attttactcg
    11101 attcgataat gcgctaagtg tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat
    11161 caaagataat ggaaaaggga tgactgaaaa acggctaaaa cagattgaag aaaaactgtc
    11221 tcatcctaaa gtggaacttc acggttctat cggtctgcaa aacgtgaatg aacgtttacg
    11281 tgccagcttc ggcagcagtt attatatgtc gcttgagaat aatgagacag gtggactgac
    11341 cgtttctatt acattcaagg agggatgagc gtgtatcaag tgttattggt agatgatgaa
    11401 tacatgattt tgaatgggtt aaaaaagata attgattggc aaagtttagg ctttcaaata
    11461 gtagcaacag cagagaatgc caaagaagga ctagctgtac ttgaacagcg acagattgat
    11521 ttagtagtaa cggatgtgac aatgccagaa ataaatggac tggaatttat tgaagcggct
    11581 caaaaagaac gtcataattt tgaatttatg attttatcag gataccaaga attcgattat
    11641 ttgaaaggag gtatgcaatt aggcgccgtg aattatttga tgaaacccgt gaataagttc
    11701 gaattggttg aaagcctcaa gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa
    11761 aatcaacaag aaatctatca agaactgtta ttcagtcaat ggttgaatga agaattggat
    11821 gaaataagtg aagaagaaat aataggacaa ataagtgaga agcagcatag gatagtattg
    11881 attcaactgg caagaactca tgatactctt atcaatagat ggttgcaaga acggcaacaa
    11941 atcttttact atcaacgaaa ttacggagac cgtgtattga tcacgttgtt gttggaggca
    12001 gatgaagttg atggcttctg ttgttttgtt caagaaaact ttcctgatca ggaatggctg
    12061 atcagtatcg gggaagagac agattctttg gacaaaatcc ctgaaacatt ccaacaagcg
    12121 aaagacaatc tacagttgca tcaattttat ggagataaag acgagcatgt tttttacgca
    12181 gtacaagcag aaacaaaaga tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa
    12241 aataaacaat tggaagtcgc tcaaaaaatg atcactgatt tttttgaaca gtttcagtta
    12301 gcagccatga tgccggaaga tatacgatat tccagttttt tactgtttat ggagattcaa
    12361 cgggaattga ttgatctgga agatgaggaa tatttacaag gaatcgaaaa aatccatcag
    12421 gcaaaaacgg tgcaagaact tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga
    12481 cagaaaaagt acagtgacaa tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa
    12541 gaaccactta cactcaaaga agtgtcagaa aacctgcatt taaatgtcat gtatctagga
    12601 cagctgttca aaaaagaaac gaaaaaaagt ttttcagctt atttgaacca tttacgtatg
    12661 gaaaaagcga aacaactgct tttgcacagt aatcaaaaca tcaatgagat agcaagtgag
    12721 attggctaca acaacaccac gtatttctct aaattattca agaaaatcgt cggcagatcc
    12781 cctaaagagt accgagaaaa cagtggagaa tcataaaaaa aaaagccaat gtaatgaaat
    12841 aacttataaa aaattgtcta gaaatctaat tttgggctgt tcttattact tgaggcaagg
    12901 cagctttttc atgacctctc gataaacgga gttcaaaagc cagtttctcg gtattaagcc
    12961 aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt tttcttaata ctcgaaacta
    13021 aacagctttt tcacgacctc tccactaaat ggtgttcaat cagctgaccc tcggtattag
    13081 ctcaaaaaat ggcaaaatat gaggaactat tttgccattt gtctctctaa tactcaggtc
    13141 agaacgctga tttcacaacc tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat
    13201 aagaccaacc aaacgaaaaa tatgaggatc tatttttgct tggctgttct tattacttga
    13261 tgcaggacag ctttttcaca acctcttgtt aattatagtt tgataaggaa aaaggcaaat
    13321 cttttgcttg atatttctta gggtgaggga tgatccctaa ttggtaagag atcgtgccgc
    13381 ctttcagcaa atcttgatgg gtaaaatata atttcgtata ttctctgtcc atccgtttga
    13441 ttttatggat aaattgttgc tgcggccgat taggcgttgc ttcaatcgtc aattcctttc
    13501 catttgacaa aagcaaaaca gccttgtcaa ttaatggcat accgataaca tattcaccgg
    13561 ttccaggcgt gacaggataa aatcccagac tattgaagat ataccaagcg gccatacttc
    13621 cattgtcctc atctccaggg taacccattg gagaatcgtc gaaggtctga gtcatcaact
    13681 gtttaagcag tggctgtgcc atttcaggtt gtcctatata actgaacaag taaggatagt
    13741 gaaaacttgg ctggtttgaa atagctaatt gtccaaattc tatcgcggcc atctcactca
    13801 tttcgtggat ctcaaaacca tatcctcctg ggtcaaacgt tgggggctga ttgcacagct
    13861 caatgagttt ttctttatat ctctcactgc caccatattt tccgatcagt cctgaaaaat
    13921 cgtgataaac agcaaagctg ctttgccaag cacttccttc tgcataatct tgtccccaac
    13981 gattagctga aaatggttct ctgaacttgc catctttatc ttttgaacgc ataaatcctg
    14041 actcatagtc aaagatattt tgatagttca atgattgctg ctgatatttc ttcgtcattt
    14101 cctcttcttc tagtaccttg gcaacttggc taatacaaaa atcgctataa gcatagtcta
    14161 atgtgtgatt gactgattcg tggtagtgat taggaacata gccatatgtc aagtaatctt
    14221 ctgttccttg gcgtccataa tttggatgtt tgctttgaac cgtagcacct ttttccattg
    14281 ccgttagaaa ttcaggcatc aagtctcgtc ggatatcttt tacagcagca tctgcaatga
    14341 ccgcatcgat caatgtccca ggcataagtc cacgttcatc aggagacagc cattttggca
    14401 aatagccact ttctctatag ctgttcaaaa aaccttccag catttcttcg tatttttcta
    14461 cagcaatcaa agaataaaga ggataaaccg ttttatacgt atcccaaaac ccattatttg
    14521 tatagagaac accaggtttt atagatcgac tagtcgtatc atagtggatt ttttgcccac
    14581 taggatcaat ttcataaaat gtttgtggaa ataaaaacac ccgatataaa ttatgataaa
    14641 acgtccgcaa ttgttgttga tcatggtgtg tgagctgtat acgatcaaaa taggactgcc
    14701 attgttcttg actgtgagca aggtaagtct ctttgctttg gttttgttct agttccaggt
    14761 tataagctgc ttgttccgag ctgataaaag atgtcccaaa tcggatagtt tgtttttttg
    14821 tctgttcaaa atacaacggg atcgagtcgc tatttttgtt ggaaacagct aactcatcaa
    14881 ttgttaaagg atattcaaat ctcaatataa aataaaaaga aaaatcagga tcttctgaac
    14941 cagcaaaatg tgtgattttt ccttccactg tgtaaggatc tataacaaag agctgatggc
    15001 gtcccggaaa ggataaaagt aaaccgctgt catttttgct gtaatcaata gataagattc
    15061 cgccatacat gctaggtatc aatgtagaac agatgccata acgtaattga cagatcgaca
    15121 gatgagtcgg gcaaaatata ctttcttctg ggcggtaaga actttgagca tggaaaaggg
    15181 tgttctcctg tagttttcca ttgatcggcg taaacagcat atgactgaaa tcacccatcc
    15241 aaggacttgg ttgatgggta agacggtagc cttgaaaaac gcggtcatca ggatgaaacc
    15301 accagctgcc gttttgatcc gtagtttgcg gtgcaaaata attcatccca aaaggaacgc
    15361 cagtataagg ctggcagttc ccattagaga aatttggctg attcgctgtt gcatgtctag
    15421 tatctatttg accgatattc attttatttc tcctttgttg attgtctttt gatgaatgaa
    15481 actggaaatt tactatcgga tggtcgatct tttgtctcta accaagtgac caaagcatct
    15541 cccgcaagct tacctaactg ttcaaaatct tgtgcgatgg ttgtcagtgg cgggtcaaca
    15601 agagctgctg cttggatatc atcaaatccg ataattgaaa aatcttcagg gatatgataa
    15661 ccatgctgtc tcaagagatt catggtctgg atagcgacaa catcgttttc acaaacaaaa
    15721 gcagttactt catacttagt gatataagaa agcaattcat ctaatgtcgc gcttttgtca
    15781 tccagtgaag tgtggaaagt caaaccagct ttgtccaatg cttcaaggta accaaggtaa
    15841 cgctgtcgaa cggattgtgg atgagtctgc gtaccaaaca aataagcgat ttttgaatgt
    15901 ccctgttcga tcaaataaga ggcagcaagc gcaccgcctt ggaaattatc ggaaagaacg
    15961 gtaggaaaag gtaactcata gattttttta tctaatatga cgacagggaa ttctttcaat
    16021 gaaagctcaa aaagtaagtc aagatgttgt tctgtatcaa gagcataata aatcaatccg
    16081 tcaaattcat tgattagctc ttttgctgtt ttattttgta gaaagtcaag cgtcgtcatc
    16141 atgacatcaa tctgtttatc ttgcatcaca gggttcaatc ccttgctaaa atcaccaaca
    16201 gacaaatcag cgacatatgg aagaagaaac agaatccgtc gtgtttttgt aggagaagca
    16261 agaatctgtt ccggagaagc ggatttaacg aaactgcctt tcccgcgaac gcgataaata
    16321 agtcctaatt gctctaattc agtcaatgca cgtttggaag tgattctgct tactttatag
    16381 gtacgtgaaa gttctttttc agttggtagt tggctgtcta caggtaattc accagttaaa
    16441 atagccgttt gcaaatcatt aaatattgtc tggtacaatg gttgagtcat ataaacacct
    16501 cttaatgata tatcatattc aaataatacc ataatggatg aaaaagtaaa taaaaagata
    16561 ttttttgaaa gcgtagacat aattggctgt atgaagtata atctttaaag atatatcaaa
    16621 tagaaagaag gttttattag tggtttataa agatatacca aaatcggtgc agaaatttat
    16681 ggaagatatc acacaaaaat gtggggatat ccatgcggac tgggcaaaga attttacagc
    16741 agcatttgcc aatactctgt tgacaacagt caaaaagcaa aatgacggaa ccaccttttt
    16801 attgacggga gacattccag cgatgtggtt aagagactct actgcacaag ttcgtcctta
    16861 tttagtgatt gcaaaagaag atgaggaatt agctgacatg atcagcgggt tagtcaaaaa
    16921 gcaatttttt tatatcaata tcgatccata tgcgaatgcc ttcaacgaag aagctaacgg
    16981 tgctggccac caaacagatc atacaaaaat gaacgattgg atctgggaaa gaaaatatga
    17041 aattgattct ttatgctacc caatacagtt ggcctatctt ctttacaaaa atacaggaaa
    17101 aactgatcaa ttcaatcaat cttttattga aggtatcaaa aaaatcttgg acgtgttcaa
    17161 aacagagcaa gatcatgaac actctcctta tacatttgta cgagacacga caagaaaaga
    17221 agatacgctg atcaatgacg ggcgaggtgc aaaaacagct tttacaggaa tgacatggtc
    17281 tggtttccgt cctagcgatg atgcttgccg ttatggatat ctggtaccat ccaatatgtt
    17341 tgcggtggtc gtgttaggtt atgtggaaga aatttttcaa acgatactcg atgataaaga
    17401 aattgtacag caaacacaga aattgaagaa agagatccaa gaggggattc agaatcacgg
    17461 acgaacggtt aatcaaaaag ggcaaacgat ttatgcatat gaagtagatg gaatgggaaa
    17521 cgccacagtc atggatgaca gtaatgtgcc caatctgatc gccgcacctt acttgggata
    17581 ttgttctgaa caagaggaag attatctgca aacaagaaga acattgttga gtaaagaaaa
    17641 tccatatttc tatgaaggaa agtacgcaaa aggaattgga tcctcccata cgccagaaaa
    17701 atacgtttgg ccgattgcat tagctatgga aggaatgaca acgtcagaca aatctgaaaa
    17761 agagcggata ttgaatttac ttgtagcaac tgacgcagga acacacctta tgcacgaagg
    17821 attcgatgtg aacaatcctg aaaattatac gagagaatgg ttttcttggg caaatatgat
    17881 gttttgcgaa ttagtcatgg attactttga tattagagta gaaaagtagg aggtatcgta
    17941 aatgaaaaag aaagtatata tcatttctca tagccactgg gatcgtgaat ggtacatggc
    18001 ttacgaacag catcacatgc gactgattga attgatagac gatctactag agctatttga
    18061 agttgatccg tccttcaaca gttttcattt ggatgggcaa acgattattt tagatgacta
    18121 tcttcaagta aggccagaaa aaagacaggc aatccaacaa gcaatcaatg aaggaaaatt
    18181 gagaattgga ccattttata tcttgcaaga tgattttctt atcagctcgg aatcgaacgt
    18241 gagaaatatg ctgattggca tggaagaaag ccgtaaatgg ggaacccctg tcatgttagg
    18301 ctattttccc gatacatttg gaaatatggg acagacgcct caattaatga aacaagctgg
    18361 tatttcagca gcagcgtttg gccgtggtgt aaaaccaatt ggctttgata atcaagtttt
    18421 ggaagcagaa aactattctt cccaatactc agagatgtgg tggaaaggcc ctgatcaaac
    18481 agcaatcttt ggtctgctat ttgccaattg gtatagcaac gggaatgaga ttccagtaga
    18541 aaaagaagca gctcttgcct tttggaagca aaaattagcg gatgcagaac aatatgcctc
    18601 tacgaaccac ttattgatga tgaatggtgt tgatcatcaa ccggtacaaa aagatatatc
    18661 aaaagcaatc catctcgcaa atgaactgtt tccagattac gagtttattc attctaattt
    18721 tactgactat ctggaagcag ttcaaaaaga tgtgccagaa gacttaggct cagtagaagg
    18781 agaattgaca agtcaagaaa cggatggctg gtatacttta gccaatacag catctgctcg
    18841 ggtttactta aaacaatgga ataccaaggt ccagcgacag ctagaaaata ttacagaacc
    18901 acttgcgacg atggcttatg aagttagtgg aaactatccc catgatcaat tagattatgc
    18961 ttggaagaca ttaatgcaaa atcatcctca cgacagtatt tgcggttgtt cagtcgattc
    19021 tgtccatcgg gaaatgatac ctcgttttga aaaagcagat gaagttggaa agtatcttgc
    19081 acaagattcc ttagaacagc tgaccgcagc aatcgatacg accggattcc caaaagacag
    19141 ctttccattt gtcattgtaa atactgcagg aatggataaa actggtgaag cagaaataac
    19201 catcgaatta gaaagaaaaa gattcgctga aggaatacct gaacaactat atcaagagtt
    19261 agaaaatttg cctaaacgta agtatcatgt agaaacaaaa agtggggcca ctataccagc
    19321 tattttatct gaagaaactg tccaatttgg ttatgatttg ccgaaagacc gtttccgtgt
    19381 gccatatatg gcaagaatga tcaaagtgac tttaccatta gaaaacatgc cagcattttc
    19441 ttgggaaaca ttcgcgcttg tagaagggga agcgaaagca gaagaaaagg aaacaatgat
    19501 tcatcagagt ggacgaatca tcgaaaacgg ccctctacat ttgacgattg aaaaaaatgg
    19561 taccattaca atggaagatc gcaaaaacaa acgaaagttg aataatctgc atatttttga
    19621 agatatcggc gatataggaa atgagtatat ctttaaacaa cctttctgtg atcagccaat
    19681 cctatccagt aacaaagaaa actctgaagt aaaagtatta gtagatacac cggaaatagc
    19741 aaaaataagt attctacagg aaatggaaat ccctgtttct gcagacgagc gtttagaaaa
    19801 agaacagcag atggttgtgg aatttcgtta tcgaaaagca gaacgatcca aagaaaaacg
    19861 aatccttcaa ataaaaacaa tcatgacaat cagaaaagat agtaaaaaaa tcgactttga
    19921 aacgactctc gacaatcaga tgaaagatca tcgcttacgt gtcttgtttc ctacaaagtt
    19981 acatgtggaa catcacgaag cagatagtat ttttgaagtg gtgaagcgcc caaaccatgt
    20041 gtctaaaagt tgggaaaacc ccacgaatcc gcaacatcag caagcatttg tcaatattca
    20101 tgatgaagag tacggtgtaa cagtcggaaa ctttggtttg aatgaatatg aagtaacaga
    20161 agacggacaa atcgcagtga cgctacttcg cagtgtggga gaactaggcg attggggata
    20221 ttttccaaca ccagaagcac agtgtctagg tgagcatcgg ttcaattaca gcattgaact
    20281 acatggacca gaagaaaaat tctctactta tttgcatgca tacgcagcac agattccttt
    20341 ttccactcaa caaatcaaac atcatgaagg aacattgatt tcaaaacaac aatatttaac
    20401 gataaaaagt gaaacgtttg caattacagc tttgaaacgc agcaagttta gtgataaagt
    20461 agtagtaaga ggtttcaata tgtctagtca tttagaaaaa ttggaaataa ctaaagacaa
    20521 tggaaaaact gtcatattga atctgcttga agaaccaacg aagcaagcgg ttgtcccaat
    20581 aatccaacct tatgagattc ggacaatcgg attcgaggag gaaaacgaat gtatggcgga
    20641 atagaagcag gcggaacaaa atttgtgtgt gcggtttcag atggtggaga aattttagaa
    20701 aaattaagta tccctaccac aacaccagaa gaaacattgg atcaggtatt tgaatttttt
    20761 gatcgttatg aattgaaggg aatcgggatt ggctcattcg gcccgattgg tatcgacaaa
    20821 acaaaagata attacggata cgttttggca actcccaaaa aagggtgggc acaattcgat
    20881 tttcttggca caatcaagga acgctacaat attcctgctg cttggacaac ggatgtcaat
    20941 gctgctgcat atggggaatt gaagcaagga gcagcacaag gaaaaaatag ttgtatttac
    21001 cttaccgtgg gaacaggaat cggtgcaggt gtcgtgatca atgaagaaat tttttcaggg
    21061 atcgcacatc ctgaaatggg acatatttgg gtgaaacgcc atccagatga ccattatgaa
    21121 ggtacttgtc cttatcataa agattgttta gaaggattag cagcaggacc gtctattgag
    21181 gcacgcacag ggatcaaagg gcaagatctg ccgcaagatc atcctgtttg ggctattcag
    21241 gcttattata ttgctcaagc actgatcaac tatacgttga cgttagcacc tgaaaagatt
    21301 attttaggcg gtggggtaat gaatcaggat catctccttc aaaaaatccg tcagcaattt
    21361 gttgaattga tgggtggtta tatggaaaca cctcaagtaa gtgaatatat cgttcgttgg
    21421 ggactgccga atgaaagtgg gattatcggt agtttgctgt tagcagaaca cgaacatcaa
    21481 aagtaagcag acaaattaca caaaaagaac agagtcttat cattatctgc tgataagact
    21541 ctgttctttt ttatttctat tcccataatc caggtaaagc tttaccgact attttttctt
    21601 gagtaaatgg atggatcatg tgcagttcta atgcatggag catcagtcgt tcagctgttc
    21661 tattttggta gagagggtct ccaacgatcg gatgtccctt atgagctaaa tgaactctga
    21721 tctggtgtgt acgtccggtt tccaaaacac aatagacttg tgtttgttca gaaagtactt
    21781 tgttgacagt gacatgtgtc aaggcgctct gaccattttt atcatctacg accctttttc
    21841 tccgatcatg gcgatctcgt ccaattttat tggtgatggt catatctttg gcaatttctc
    21901 cccatacgat ggcttgatat ctccgataga tcattttttg ttccaacatt cttccaagta
    21961 tcggcaagac aaatggattt tttgcaaata aaacggcacc gcttgtttct ttgtccaagc
    22021 ggtggacaac gtaaggtcgt tgctgtttag tgtcgagata agcagctaaa tgattgagta
    22081 aggtactgtt ttctgtcggt tcatttggat gagtcttcat tcctgcaggc ttatccacta
    22141 taatcaaatg ctcatcttca tataaaacac ggatgtgctt cgcatctcct aattgaactt
    22201 cttgataatt ataatctgtt tcttcgaata acaatgtgat ctggtcattg ccctttactt
    22261 catgatggaa caaagcaggt tgttgattga tccaaacgtt tttacgtata cgcaaaaagt
    22321 gtcgaacctt tctaggaact aaccattcct tttcaagcaa gtcgcgaatt gttgtttcag
    22381 gatggttcat aggtaaagtg atcgtgattt tcacaagtgt actcctttca gtgcaatcgc
    22441 agactaattg taccattttt aaaacatatt taagaaaaaa cttacatttt tctgttcatt
    22501 cttatgttac tctacttgca ctaacgtaaa aaaacatatt gaactacaac ttaagcagga
    22561 agaaaacact ctttcactat ggtaaaataa gacgattgaa tcaaatagac aggagaagaa
    22621 gcaaatggat tttcaaagca ttttagaaaa gataaaagct gctttgatca gtttttggcg
    22681 ttggatcaga ccatatctcg gacaattcca tcgatggcga aagcgcatct ggaaaaaata
    22741 tcatgtgaac aaattgattt tgctgattgg tttgatctgt gtcttagtga caagtatcta
    22801 cctctttatt ttagcaaaac aagcaaatgt cgaaacgtta aaatcaggat taagccaatc
    22861 gacagtcatt tatgataaga aaaacgaaga agctggcaca ctgtacgccc aaaaaggaac
    22921 atatgtagaa ttaaacgcga tttctcccta tatccaagat gcagtaatct cgacagaaga
    22981 taggaatttt tacgagcatc acgggtttga tatcaaaggg attgcccgtg cagcagtccg
    23041 aatggtcttg tctggcggaa catcaggggg cggaggaagt acgatcactc agcagcttgc
    23101 caaaaatgcg tatctgacat tagaccaaac gtttgatcga aaagcgaagg aattattttt
    23161 ggcaatcgag atcgaaaaga agtacagcaa agaagaaatc ttgaccatgt atcttaacaa
    23221 tgcctatttt ggcaacgggg tatggggtgt ccaagacgct tccagaaagt attttggagt
    23281 agatgcaacg aatgttacgc tgtctgaggc tgctactttg gcaggaatgc tgaaaggtcc
    23341 tggtatttat aatccgatcg atcacccaaa aaatgcgaat gatcggaaaa acacagtact
    23401 aagtgtaatg gaagaaaacg gaaaaatcac aaaagaacaa gaacaagcag aaagccagac
    23461 agatatcagc gcgtatctta atgacacata cgataattca gattctggtt atcgttatcc
    23521 ttattatttt gatgcagtga tcgatgaagc agttaacgag tatggtcttg atgaagaaga
    23581 tgtcatgaat aaaggctata aaatctatac agctttagat caggattatc aaaagcaatt
    23641 agaagctact tatcaaaaca gtgcattatt cccagcaaat gcttcagatg gtgccatggt
    23701 ccaatctgct tcagtagctt tagatccgaa tacaggtggt gtacaagcac tagtaggacg
    23761 aagaggagat catgtcttta gaggattcag ctttgctaca cagatgcgtc gttcaccagg
    23821 atcaacgatc aaaccattaa gcgtatatac accagcactg gaagctggat acaagccttc
    23881 cagtatcttg aaagacgaac cgcaaagtta ttacgatgcg cataattttg acgggacata
    23941 ccaaggagaa gtaccgatgt atcaagctgt tgcacaaagt ttgaatttgc ctactgtatg
    24001 gcttttgcat gagatcggtt tacaaaaagg atatgataaa gcagaagaat tcggcctgcc
    24061 tttagcaaaa tctgataagt attatggctt agcccttggt ggactggaaa aaggagtatc
    24121 tccattgatc atggcgggtg cttacagtgc atttgccaat gatggtcaga tgtatacgcc
    24181 gcatcttatc acgaaaatcg ttgattcgac tggagcggtg atcgtcgata acacaaacaa
    24241 aaaaccaaaa caggtcatat caaaggaaac agccgatgaa atgaccagca tgcttcttgg
    24301 tacattttca aatggaacag cggctgctgc gaatcctgca ggttatacga tagcagggaa
    24361 aacaggaacc acagaaacta atttcgatgc cacaaaagca aatgaccaat ggatgatcgg
    24421 ttacacgcca gacgtagtga tttcgacatg gatgggttac gaagaatcca gcaagctgca
    24481 ctatttggaa gggacaagtg gaaatgttgt cgggaaagtc ttcaaatcag cggcagaagg
    24541 catccttccg tatacttcaa aaacaggatt tagtgtagca gatgcctatg ccacaggagg
    24601 acaagtggta gctgcagatc aagttggaaa cacaggaaat tctaatggag agtctggaaa
    24661 ttggcaagat aacttgaatc aatatggtga acaagcaaaa gaagggctga aaaacttcgg
    24721 cgatatggtg aaagaaggcg ttcagggaat tggtgaagcg gctaaagacc tctggcggaa
    24781 atatcaagga gagtagcccc tttgaaaacg aataacccca tgttacaata gagagtaact
    24841 actaaaagaa aagagggcat aacatgtcaa acatctacga tagcgcgaat caaatcgaac
    24901 gtgagatccg tgaattacca gaattcaaag cgttgaaaga agcgtacgag aaagtacaag
    24961 caaacgaaga agcacacaaa ctgttcaaag acttccaagc gatgcaaatc gaattacaag
    25021 aaaaacaaat gagtggacaa gagttcagcg acgaagatgc aatgaaagca caagaaatgg
    25081 ctgtgaaaat ccaatcagaa gaagtaatca atgacttgat gcaaaaagag caaggcttca
    25141 gtacaatcat caatgacttg aaccgtatca tcatgacacc agttcgcgac ctttacagcg
    25201 aataaaacta ttttatgaaa caacagaggg tgtgaaagag acgttcggcc ttgagcgatg
    25261 acaaaattgc ttcttattgc cgataggctg tctcttgaac accatttacc aagaggtcgt
    25321 gagcctaccg tttaactctg agttacaagg aacaatttca aaaaacagct tctcatgttt
    25381 gatgactatt gtgacttgtg accgaggagt tggcaggaga acaccgttta ttcagaaata
    25441 ggagctacga caaaagtctt gtcatagctc ctattttttg tcattcattt ttcaagcggt
    25501 agaaatagcc tttttgagct aacagttttt cgtaagtacc tatttcaacg atttttcctt
    25561 catcgatcac aacgatttta tctgcctctt tgatcgttga aagccgatga gccacaataa
    25621 aagtagtggg tgtttctgtc aggtaattca gggcttgctg gatttttttc tctgactggt
    25681 tatctaaagc tgaggttgct tcatctagca aaatgatttt tggctgacgt atcaacgctc
    25741 gtgcaatgga gatacgttgc ttttggccac cagacagctt gttgccatgt tcgcctacta
    25801 aggtatccag accatcagga agctggtcaa tcagtgaaga aagttgcgca gcttcgatga
    25861 cttcatctaa acgttctttt gatacgtttt tcaagccata agtgatgttc tctttgatcg
    25921 aagcggagaa caacaaagtg gtttgaggaa caacggctaa ataatttcgc acacttcgca
    25981 tatccagttc atccaaaggc tgatcatcta gaaaaatttt tcctgaagtc ggctggataa
    26041 atccaatcaa taagttaagg agtgtagatt ttccagagcc agatggccca acaaaagcaa
    26101 ttgtttctcc agctttcacc tctagagaaa aatgatcgat aatcggtttg agtgagtccg
    26161 gataaagaaa agaaacatcc tcaaaacgaa aatttccatc caaatgttgg atttttttct
    26221 taccgtgata agtttctaca tcgccagcaa tcaacagttc gccaatagaa gtgatagatt
    26281 ccatgccttt tgaaatcgta ggaatcagcg tgatcattcc agaaacggaa ttgacgatcg
    26341 tcgtgaaata cgtctgatac atcacgacat cgccgggttg gatgcgccct tgtaaagcta
    26401 atatcccagt gaaaatcaaa caaatcagct gaaatatctg aaaactagcc cagctgactg
    26461 atccgaaaac agattgcagg atatctaagt ggaatccttt ttcatagatt tgagtaaaat
    26521 gcttatttaa gcgagttgtt tcgtgttttt ctaacccatg agctcgagca atcggtgcta
    26581 attcgatcac ttcgatcact tttgcagaag tctcttccat ttcttgcctg aagtctgagt
    26641 tggactgtct gatcctttta cggaaaaaga gagtaacgaa tgcggcgaga ggaacagtca
    26701 ataagaaaaa catgaaaaca acaggacttt tataaagcgt gatacctaaa gctacgaata
    26761 aattgacagc gatatttaat ccgctgacaa atacttgaga ggacaacgtt tggatagctt
    26821 ctacatcacg gatgatcttt gactggattc gtccagattg caattctttt tgataaggaa
    26881 tagatagttc ttggattttc ttgataaggg tcgctcttag tccagcttct acttgacgga
    26941 tcgaagtgct atggtatttt acatgaaaat aattcgtcaa tatattttga ccaatcagac
    27001 aagccatcaa aaaagcgttg aaataaatgg cctgcaactg agaagaatct ttcgatgaag
    27061 ctgcattgat tacattggca atagcgatcg gcattaacca agatggaaga tgtttgataa
    27121 taaaaaagag tcctgacatg gcaaaacgcc aataatttcc ctgataaaga tgcagcaatg
    27181 tctgaaaagc atttgcatga tatttttgat aacttttctg tattcgttcc tccataatct
    27241 tctccttaac aatcttggta aataaactgc aagtgttcta ttgcttcagg aacataaatc
    27301 tcaggagtag cttcaagagt ggcgtacatt agaggacgct catatttgat ataatgcagt
    27361 accgggcgga aatctaaaca gcccttgcca acaggtacca tgcggatctt tccattttcc
    27421 atgatatagt ctttgagatg aagcgctgta atataaccat gtaaattgtc caaggcacct
    27481 cggatcactt tttcctgatc agcatgattt tctttatgca tcaaattcgc acaatctaaa
    27541 atgattttta aatttggtga agctacttca tcgattaaac gtttagctaa ctgatagtta
    27601 tagattggat gattgatccc tgcttcaaca gcaacagtta cgccaaaatt ttctgcatga
    27661 gcgactaatt caatgataga atttctggcg atctgataag cttcttcagt aaaattttct
    27721 tttgtgtaac cgacagagac actaccggtt tccgtagcaa tcatcttggc ttgataggca
    27781 gagcacagag caatttgctg tttgaaagtt gccaatgctt gtgcacgaac ttcttgattt
    27841 ttgctggata ggttgatata gcatcctaaa atggaaagtt cgatattgtt tttttggaag
    27901 tattcaccaa aataggcagc actcccaagt gtcatgtcag ttaatttaag ttgttcagga
    27961 aatgcttttt gtggtgcaaa ttggatatgg gacaagtgaa gctgtgatac tttttgaacg
    28021 atttcttcac gatttcgaac atctaaatcg tgggcacgga tgcctaaatt taatggaaac
    28081 atctcaaact cctttttgta aataattagc aagaggcaga cgggattctt ttatcaattc
    28141 aacaattatt cgggcaactt tatcagcgcc ttctggacta aagtgggtat tgtcttttag
    28201 tccttctggg taatttttac aagtatcagg tataagatgc aggtagaatt tttttgtctc
    28261 ttcttttgag aaggtctgga acaattcttg tgatcttgag aacacatcta aaagaggaat
    28321 atggtgtttc tcagcaaatg cacgcatcgc ttttggatag tctccaagta tatctggatt
    28381 caatgttccg ttttctaagt aatctcgtcg cgtcacagat gtcagtaaga ccatttggac
    28441 gttcgctttt tttgcgcatt caaaaaatat ctgcagattt tttaaatagc taccgaaggg
    28501 aagcgtacct ctttcttctt ggattttctg gtcgttatgt ccaaattgta caaataagaa
    28561 atcattcggc tgaaactgtg aagctagacg ggctaacctt ccttcctgga taaatgattt
    28621 ggtgctgcgt ccattctttg cttggttatt gatctggatc tcgggtgaaa aataagcaga
    28681 gattttttct ccccaacctg tctcgggacg cttgttttca tctttttctg ccgcagtaga
    28741 atcaccggca atatggatag taggcatgtt atctcttctt tcataaaggt ttataggcaa
    28801 tgaagcagaa cagcaagaga agaagacgga gcaaatttgg tccgtcttct tctctaaaac
    28861 gtggataaac ggattccgtt atttcactat cctgtttttg attcgttact gttactatta
    28921 atcaattagg caaaaaagcc aaagtaattt ttagcatttg tataactaat cgcttgaact
    28981 agacttccta aaagttgttc atcttcaggt gcttctcctc gcatgaccat ttcgcctaaa
    29041 aattcacaaa gaacacgacg gaaatattca tgtctagtgt aggacaggaa acttcttgaa
    29101 tctgtcagca tgccgacaaa attccccaaa gcactaccat cagcaaattg tgtcaattga
    29161 tcgcgcatac cagctcgagt atcgttgtac caccatccag aacctaactg cagttttcca
    29221 ttctgttctt tttggaaaca gcccatcaaa gctaaaatcg caggatagtc attcggattc
    29281 aatgaataaa ggatcgtctt tggcaactga tccgtttctt ctgcacggtt caataatttt
    29341 tgcagaggaa tagtcaaaga aagatcattc attccatcat agccggtatc gggccctaat
    29401 ttttccaaca tttgtgtatt acagttacga taagcatgga tgtgcagctg catcgtccaa
    29461 ttatgagaat gatagaagtg ggtcaaatgt atcagtgttt ctgtacgata agcatcgatt
    29521 tcatctgaag ataatagttt gttagctcgt gctttttgaa aaatctcttc taatactgtt
    29581 tcatccgctt ctttatagcg taagatgtct aaagcgtggt cggaaacacg acatccatgt
    29641 tgatggaagt attctatccg ctgccctaag acttctatca gttcttgata ggtggtgatc
    29701 gtttggtcag ctgcttcttc cagtagtgtg acccatgcat taaatccttc tttttcaata
    29761 ttgagtgctt tatctggacg gaatgaaggt agaaccttca aatctggttc ttcatgagca
    29821 agcaactgat gatagtgcaa gtcgtctgcg ggatcatctg ttgtacaaat cacttctaca
    29881 ttcgattttt ttgcgatttc ccgacggcga aaatcaggag ctgccaaaat ttcatttgct
    29941 tgtgtccaaa ttttctctgc attttcttca tttatcaaca agtcaatacc gaagaaacgc
    30001 ttcaattcaa gatgtgacca actgtaaagc ggatttccga taatttttgg tacggtttga
    30061 caccaagcta aaaatttttc aaaatcagaa gcatttcctg tgattttttc ttcaggcacg
    30121 ccgcaagctc gcatcaagcg ccatttgtaa tgatcgcctc ctaaccaagc ttctgtcaga
    30181 ttgctgaaat tgttgttttc gtaaatttct tttggatcta agtgacagtg ataatcaata
    30241 atgggcatgt tttttgcgta ggaatggtaa agctttttcg cccaatcgtc ttttaataaa
    30301 aaatcatcag ataaaaacat aaatcttcct tctttctttt tatactgttt tatgaaaatc
    30361 aaagtgttac tccgttttta aaaatggcaa tttctcgaac gtcattttgt tcatttttcg
    30421 tttcttctcc actagctaca gcgatgattt tttgaacaaa cgattctaag attacttcca
    30481 ttggttgttc taataagacg cctgcattaa aatccatcca atgacctttg cgttcaaaaa
    30541 gagttgtatt cgtagatacc ttgaccgttg ggacatatga gccaaacgga gtccctcttc
    30601 ctgtggtaaa caagaccaac tggcaatcgg atgaagcaag agcagaggca gctacaagat
    30661 cattcccggg agcttgcaat aaactaagcc ctggttttct gattttttcg ccatattgta
    30721 gcacatcaac gacaggagag cttcctgatt tttgcgtgca gcctaaagac ttatcttcta
    30781 atgtagtgat cccacctgct ttattaccag gagatgggtt ttcgtaaact ggttccccgt
    30841 aagacaagaa atactgtttg aagtcattga tcaagtcaac gattgagtca aaaacttctt
    30901 cattttccgc tcgtgccatc aatacttgtt ctgcgccaaa catttctggc acttctgtta
    30961 agatcgtgct gcctccttga gcaatcaagt agtcagaaaa tgcaccaagt aaaggattag
    31021 cagttattcc cgatagacca tcagaaccgc cgcatttcaa accgacattc aacttagaga
    31081 gcgggactga tatgcgctgg tcattttccg ccgctgttaa aagctcttct aatagagcca
    31141 caccttgttc gatctcatcg taaacatctt gagccactag aaatttcact cgttcttcat
    31201 catattcacc taaaattttt ttgaattcag gaactgtatt gttctcacaa cctaatccaa
    31261 atactaaaac tcctccggca ttgggatgat ttactgcgtc cgctaaaatt tctcgagtat
    31321 tttgatgatc ttttccaagc tgcgaacagc cataaggatg ctttaaaatc gtaatatggt
    31381 caaactgtcc cagatctgga tgtttttcct tgaactgttt gacgatcagt tcagcaatgc
    31441 cattcacaca cccaacggta ggtactaaaa acaaatcatt acgaatccct acttttccat
    31501 tttttcgcaa gtaaccttga aatgttcggt tctcaaaaag gtaatgactt ggatggacat
    31561 ctttttcata tcgataatcc aattcaccag aaagattggt ttttacatta tgagtgtgca
    31621 gccattgacc ggcagttacg tctgtcgtca catgaccgat tggatagcca tatttgatca
    31681 cgttgtctcc agctttcaaa tcacccaatg caatcttatg accttgcggg atatcttcaa
    31741 gagtggtgat cgtttgctga tcaatcgtca gttgtgtttg ttttggaata ggggttaatg
    31801 cgacgaccac actgtctcgt cgatttaatt tcattgttgg ttgcagggtt tcaagcatgg
    31861 accatcgttc ctttcatttc ttgtaatact gaccggacac ctttttgatc gatctttgca
    31921 gctatttctt gaactgtatc tgtcacattc ggtatttgag acaaatcttc tccccaaagc
    31981 gcagcgtttt gcaataatcg aggaatagct gttccattat cagaccaagc atcagtaaat
    32041 tcgcttatcg tcttttcatc atcatgtggc tttacttgat cgcctcgata cataagaagt
    32101 agcgcagcta gtgaaacagt gattaacggt ggtacctgat tttgttcttc gatatacttc
    32161 ttgaacactg gaagcagacg ggtgctgaac tttgaaacac tatttaatga aatcgcttct
    32221 aatttgtgat gggcaaaagg attcaagaaa cgttctttga tttggtcagc ataaatggct
    32281 aattcatctt taggtaaaga aagcatagga atcaattcta gattgaataa ctgatcaaca
    32341 tatgcggaga ataaaggatc tttcattact tcttctacag tttccaattc cgccaacaaa
    32401 gcaagaggga ccatcgctgt atgcggtcca ttaagcaaat gcaccttacg ttcacgataa
    32461 ggagtcatat catctgtgat gatgacattc aagccagcct gtttgaatgg tagttgattc
    32521 tccaattctt taggcccttc gatcacccaa agcatgaatg gttctgcttt gaccatcaat
    32581 tgatcttcat agttatgccg ttgattgaaa acacctgcat cttcgcgagg ataacctggg
    32641 acgatgcgat caactaaact acaacagaaa acattttctt tttctaacca ctcaacaaaa
    32701 gcaggttcta aatcccattt ttctgcgtag cgtaaaatga tttcttttag acgttcgccg
    32761 tttctgtcaa tcagttcgca aggaatgatg gtaaagccag ctttgtttaa tttgaaacgt
    32821 cggtataaaa aggctgtcag tttgcccggg aagctctgtg ggggagtatc tgttaaatta
    32881 tcagtctcca cgaattggat tcctgcctct gttgtattgg agacgatata ggccaagtcg
    32941 tcattttctg ctagagatag ataagcatcc cattcatcat aaggattgat catttgttct
    33001 actacggtga taatctcgga agtatcaacg atttcgccat tgctgagtcc ttgtaaaatg
    33061 accgtatata aattatcttg ctggttcatc atatttccaa gaccttgttc taaaggttga
    33121 atgattgcaa cattaccttt aaacaattgt tggttattca gctgctgaat ctgccaatca
    33181 ataaatccac gcataaaatt gccttcacca aactgaagaa cttttacagg tgcttcgttt
    33241 tttttcacta attcttttgt cagttttttc atataaactt cctttctgtg ttatgcacac
    33301 gttaacattt ttatataaaa ttacttgtag tttacaagta attgatcgac tcacgaagga
    33361 tcaacaaggt ggagagctca attttagatt gttcgatcgt cctgccttcg atcaaatcta
    33421 gtaaaagctt tgttccttct tgacacattt ctttcaaagg cttacgtacg gtggtaagcg
    33481 gcggattgag ataggagacg actggagaat catcgaaccc gatcagagaa atgttctggg
    33541 gaacacggag gccagctaaa tgtacagctc tcaatgcacc aatcgctatg tcgtcgttgc
    33601 cgcaaaaaac cagagtaggc aggtcagaaa gtgataatag gtattccatg ttttggaggc
    33661 cgctttctaa gctgtaatct cctgttaaaa aataggctgg attgacaggg atgctcgctt
    33721 tttttagact tttcaatacc cctttttccc gttcttttgt cgaacgaaaa tttttctttc
    33781 cgccaatata agcgatcttc gtatgtccgc atccaatacc gtaatctact gcctgcataa
    33841 caccggccgc atcatcggca gtgacgttga taaccgctgg atcattgatg tgacgattga
    33901 caacaacgaa aggaaattct ttttctttga gatagtcgat gaaaggctga tcatcatcac
    33961 tttgactcat aacgatcagt ccatcgtagc gttgcaggtt gatttctgtt aaatcaacaa
    34021 catcgatact ttcaacagat aaactgtaat ttgtgtctaa tactgaatgg atgcctgcaa
    34081 tggaatctgc taggaaattt gatgaagttc ctttatgcaa acttgagaaa aatagtccga
    34141 ttagatattt tttttgattc actaaacctt ttgcactata gtttggcgaa taattcattt
    34201 ccttagctag ttgtcgtatt ttttgttttg tttcattttt tattaaactg ctgttattca
    34261 aagcacgaga aactgtcgta tgagaaacgc cagctgcttt agcgatatct ttgatcgtta
    34321 ccattccatc attttcctta ctttttcact ttttgttaac gcattcatca tattacgata
    34381 actacgataa gtcaatagat ctttgatatg aaaagattgc acacgttaaa aattacttgg
    34441 atttttattt ttaattatgc tattcttgaa agcggggaat gctcatttaa ttagatgagt
    34501 atcaatttta cagaaaaatg aaagcctttt attttaaaag gaggagagga agaaaatgaa
    34561 gaaaagtaca gtattttata aaatattttt accgatgttt ttattaggag ccgtacttgt
    34621 cataggattt agtttattca tttaccagaa tacctatgaa tcgatcgaat cgagttattt
    34681 aacagacaaa aagaatttgc tcaggcaagt taaaactaac gttgaatgga aaattagaac
    34741 gattgaatat tcgttttcta cgtatggttc gaccaaaaat ttctcggaca tattcaagca
    34801 tccgctgaca tatacagatt actccactta ttctgaaatc aggaaagaac taaattttat
    34861 agaaacgatt gttatggatg ataataatta tgatttagtc agtttggctg gtaaatgggg
    34921 agtaatcaat ggatcgttga gtcaactgac cgaagaggaa gttgaaaagt atcaacagaa
    34981 gtacataaat aacaaaaata atttattttg gcaaaagaaa aacaaaggaa tcgaaatggt
    35041 gatttctttg ccaatgtttg aaaaagaaaa atatgcattg ggaattgcta cgatagagaa
    35101 acatacaata gaacaagttg ttggtgaaag tggagaagac atactttcta ttgaaaacga
    35161 agttggcaac ctttttacta ctcagacagt aaaaaataaa gagcaaccaa ctgtattaac
    35221 ggacgtggat ctagattaca atcagctgaa agtgataaga gaaaaagata aaacgtatat
    35281 agtactgaaa tcagattata ataattggat ttacagatta gaagttgact ctagtgcaat
    35341 tggcgcaatg atccgcaatc tgagaatcgg gctagttgca gtgtctttaa cactggtagg
    35401 actcattggt cttctttcct acttattttc ggacagattt gcccgtccga tttctcaaat
    35461 acaggaacgt ctgaatctta aatcaaaagg attcagtggg aaagaactag atttggtcgc
    35521 tcaatcagtc agtcggatca taggagaaaa agaagcgtta agtgcacact tagttaccca
    35581 aaagccacag ctggaaacgc tatttgtact tagtttattt agaaatcgag tagaaaggcg
    35641 agaactagat cagcggttgc agcaatttgg ttatagttct caaaatgact gctattatac
    35701 agctttggtt caaatcgatc tgttagaaga tagtcacggt ggggagcgtg atttgctact
    35761 tttagcgatc aacaacatga tcacggaaat tgtgccaaag gaaaatcgaa tgctgccgat
    35821 cgttttgaat gaagaaatgc aagccactat ttatcgaatg gataaagatg atgcagaagc
    35881 aagcaaaaaa gtcatggagt attgccgact cattcaaaaa cgtgtaaaag aatacttgaa
    35941 tttaactatc agtataggca tcagtaaccg gttcgaaacg ctgaatgaaa gcaagcaatc
    36001 ggttgatcgg gcaaaagaag ccctgtatta tcgtgtaaat actggtccaa gttcaattat
    36061 tttttatcag gaaatcgttc ctgctgctca tgaaaaaaca cttattagat atccgataga
    36121 agaacaaaat cgattgtttg aagccattcg ttctggaaat caggaagtga agaatttagt
    36181 acatgaattg attgatgcat tgtttgcaca aaataaaaat cctttgagtc gggaagtagt
    36241 ttcggtccgc ttgatcaatg aactcgttca attaggacag ttattgggag tagattcaaa
    36301 aaactttgat aacatgaaac aaatttatgt aaaagcactg aacaattatc atcccaaaga
    36361 actagaaaaa cttgttttag aacaattagt tctgccaatc acagaatgct ctcaagcaat
    36421 cactgatcat gaatttaaaa atttgtcgga gaatattatg catatcgtct acacagaata
    36481 tgatcgtgat ctgtctttag atgtgatcgc tgataggttg cattataacc ctaactattt
    36541 aagtaatgta tttaaaaagg aaacaggaga aaaatttggt gattttgttc aaaatcatcg
    36601 tttagaggta gcaaaaaaat ggttgaaaga gactaacctc tccgtaaaag agattgcaga
    36661 aagattgcaa tatcgaaatc cgcaaaattt tattcgtttc tttaaaaaga aggaatcgat
    36721 tacgccggga gaatatcgaa aaaaatattt gtgaatcaag aggctgtttt tcttgtggac
    36781 tgattcaagc aataaaagaa gttagaataa ccctagtatt tgtatttatg gatagttaaa
    36841 tttcaaggct aagcacgcaa aaagaactgt gacaaaagtc ttgtcacagt tcttttttac
    36901 gaataaatgg cgttcaagag acaggctatc gacaataagg cataattttg tgaaatatgg
    36961 gaagctattt ttcaaaatcg cttcttattg ctcctgtggg ttactcgtcg caatgtttgt
    37021 gagagttttt atcatcgctc aaggctgaac gtctctttca caacctctga tacacggtgt
    37081 tccaggagta gattcttcga aaaataagcc gaaatttcac aaaaatttaa gaagcaattt
    37141 tcgtaaatgc cttcttattt cttgaagctg accacttctg acacaactac cattttgcgt
    37201 tcagtacata atcacccttt gacagaacct acgagtgctc ctttgacaaa atatttctga
    37261 acaaaaggat aaacaatcag cataggcact gttgcaataa cgataacagc cattttaact
    37321 gtttgtgctg gtggaatcac atcaacggca ctactttctg cttgcatccc acttgataca
    37381 atgacgattt ggcgtaaaag aatctgtatc ggccatttag atgaatcgtt gatatacaaa
    37441 atagcagtca tataagtgtt ccagtaagta acagcataaa aaagcgagat agtagcaata
    37501 gacggtaacg ctaatggcag cataattttg aaaaatacac caaaatctgt gcaaccatcc
    37561 atttttgctg attcttccaa actatccggc aaggcttgga aaaagtttct cataatgatc
    37621 atattcattg cattaacagc cacaggcagg atcatggccc agtaagaatt gaccagccct
    37681 agagaacgaa caactaagaa cgttggaatc atccccccgc tgaacaacat agaaaataca
    37741 acaagaaagt tgacgaaacc tcgaccaaac aaatactttc tggaaagacc gtaggccatc
    37801 agtgaagttg cacacatact gacgatcgtt ccgacacctg taacaccaat cgaaacagca
    37861 agtgctctaa aaatggtcgg cgtagaaaga atgtagcgat acgcgtctaa agaaaaggta
    37921 gtaggaaaaa ggataaactt tttcgcgacg acttcttgtg tagaagcaaa agatgtagcg
    37981 atgatattta aaaaaggaac gatacagatc aaagctagca aaattagaaa cgtattgttg
    38041 aaaagtgtaa aacttctttc tcctatagag actttctttt tcatatagat aggctcctcc
    38101 tcaaaataaa ttaaatgttt ctgtttctat atgttcaatt tatcatcggt gaaagattac
    38161 gtatataatc atttcctatg ctaagtgtca gatagaaaaa aatagaaggt ttgctatgcg
    38221 tagtttattt gtgaaaaaat acactttatc gttttgtata gcacaaaaat cattgctatg
    38281 taagcgtttt tacttacggt aaaaatgatt atatacgtaa ttcagatagt tatctatcat
    38341 gaagtcataa gcagcaaaca ttcacaaaac taaggagggg cttatggaaa gtgtaaagaa
    38401 acaagaacca tttcaactga cgctatcgaa aaaagaagaa aagctcagaa gacgaaaaaa
    38461 aataattgca ggaatcaaaa cgaataaatt tttatatgtg atgattctgc cgggagtcct
    38521 ttatttcttg atttttaggt atcttcctat ggggggactc gttattgctt ttcaagatta
    38581 tcaaccattt ttaggaataa tgggaagtcc ttgggtcggt ttcaaacatt tcattcgttt
    38641 gtttacagaa ccgacattct ttacattatt aagaaatacg ttgatcttgt tcggattaaa
    38701 tatcttcgtg ttttttccga tgccaatcat tttgtcttta ctattaaatg aagtaacgaa
    38761 caaacgattg aaaaacggga ttcaaacgat catttacatc cctcacttca tgtcttgggt
    38821 aatcattgtt tcgattactt atgtattttt gaatgttgac ggaggggtat tgaatgaatt
    38881 ccttgcaaca atgggatttg aaaaaatcag ttttctaaca tcccctgaat ggacacgcac
    38941 agtatatatc ggacaagtga tctggaaaga gttgggctgg tctacgatca tctacctttc
    39001 agcgataact gcagtcgata cacagttgta tgaagctgca gaaatggatg gagcaggacg
    39061 cttacgcaaa acatggcatg ttacattgcc ggcaattcgt ccagtaatca ttaccttgct
    39121 gattctaaaa atcggtcaga cattagactt agggttcgag catatgtact tgttattgaa
    39181 ttcattgaat cgaagcgttg cagaaatttt cgacacctat atttatacag ctggattgaa
    39241 gaatggacag ttaagtttca gtacagcaat gggattgttt aaaggggtcg taggattgtt
    39301 tatggtaatt ttagctaata agattgccaa aaagctagga gaagacggcg tttactaaga
    39361 tagaaagaga gggaaagata tgctgcagtc attagataaa ataaacggaa ggctaaatcg
    39421 tattttacag ttggtttatt tgaattttct gtggggcggt ttagtcatcg caggtttggg
    39481 agtttttact ttcggtcctg catcgtatgc gatggttgca gtgattagaa aatggctgcg
    39541 ctcaaaagaa acgtttcctt tgacgaaaac gtacttcaca tattttaaag aaaactatcg
    39601 tgaaaccttg ctgatgagct ggatatatgg cgccatcggg attgttttac ttgtggatct
    39661 ttctttcata cacaattggt atcttaggat cgctttgtat attgctgcgt tcatatatgt
    39721 gctctcagca ctctatattt tcccaatcat ggctcattat gattggaaag gatatttttt
    39781 caaaatgaaa atggcatttt tattcggatt ctcacatctt cattactcat tagtgttgat
    39841 attaagtatg gtagggattt atctgatgat catccaaaca atgccaggtg ttttaacctt
    39901 tatcggtggc agtctcttct tttccgcagt cacgtggaca gcaaattcta tattttgcca
    39961 tttggaatgg aagaactcat atacagaaaa aacagaagaa aacaaaaata ttattaaaac
    40021 agtgaaggag aaaatttatg aaaaaagcaa cgctcgtaaa attaggtctt agttttgcag
    40081 gaattctagc acttgccgca tgcggcagca gttcaagtaa cagttcttca gagacagaag
    40141 atggaaaagc taaaatcact tggttaaata ttttgcatca tgcttcaccg cctacagaca
    40201 cgattgtgaa cttgatcgaa gaaaaaaccg atacggaact gacattcaac tggattcctg
    40261 atgcttcaaa agaagaaaga ttaacaactg cattggcttc tgatgaacta ggagatatcg
    40321 ttacacttca aattgcgatg ttgaaaaact cttctgtccg caatgcgtta aaatcaggcg
    40381 tattctggga tgtatcagaa tatttaaaag actataaaaa tttaagtcaa atttctgaag
    40441 atagaatcga agctgcgagt atcgacggtg ccttgtacgg cgtaccgata caaaaagact
    40501 acgctcgtgc tggcttagtc attcgaaaag actggttgga taatctagga ctagatgttc
    40561 caactaccat ggatgagctt tatgaagtag ctaaacaatt tacagaaaac gatcctgatg
    40621 gaaatggtgt ggacgacaca gttggttttg gcgatcgtgc gcctagtgat attcgttata
    40681 ccagctttaa acttttcact tcttattttg gagcaccaaa tggctgggct gtagacgata
    40741 atggcaaatt cactccagaa tttgatacag aagagtatat gaaagctttg aatttttcaa
    40801 gagatctatt tgagaatggc tatttggcac aagactttgc agttacccaa aagactgatc
    40861 agcaagaaca atttgcacaa ggaagaactg gaatctacac tggaatggtg gatatcaaga
    40921 atctgaagac gatgggtcaa ggtattcaag atgatatgga attagtgcct gtaaacaaga
    40981 tctcagatgg tgaaggcggc ggttatcatg tttggtcaga aggaaatggt attggcggct
    41041 tgctagcatt tcccaaatct caagtaaaag acgaagcaca attgaagaaa ttacttcaat
    41101 ttgttgatga tcttttagaa gaagatgtgt ataaattgat gactggcggt atcgaaggaa
    41161 cgcattatga attgaatgat gatggttcta tcgaatttat cgatatggat ctttggcaac
    41221 aagaggtgca accgttatca agctctcgtc ctagtgaagt gacgttctct ttcaaagata
    41281 cagaccctga aaaagaattc tctaatcaat tgatcaaaga aaatgaagaa tttgctgtaa
    41341 tggatccttc tgtgccattg gattcaccga cgaacaatga agtaggatca gaaattgaaa
    41401 aaatcgtcat tgatgctacg gtgcaataca tcatgggaca gcttgacgaa gatggattca
    41461 aaaaagctgt agaagactgg aaagcacaag gaggcgatcg aatcacacaa gagtacgagg
    41521 aagcatacaa agcagcggca cagtaatata gacgattggg acaaatactt cttggtattt
    41581 gtcccaaatc ctattttaat ttttaaagga gagcaaataa tggaaaactg gtcaaagaaa
    41641 ttagcaaaaa gtatcatgga aagaacacct cgcttatatg aagaaaagtg gtataaagga
    41701 aaatggtctt atgactatgg cgtcgtactt aaagggttcc aattactttg ggaacgaacc
    41761 caagaaaaaa tttattttga tttcataaaa gacaatatcg actatttcgt gcaagaagat
    41821 ggaacgatcc gtggctacag cgttgaagaa tacaatatcg accatgtaaa tacaggaaag
    41881 cttttctttt tattgtacaa agaaacaggc gaagaaaaat ataaaaaagc agcagaactc
    41941 ctttcccagc agttggcaaa ccatccacgc acatccgaag gagctttttg gcataaagaa
    42001 atttatcctt atcaaatttg gttggatggc ttatatatgg gttctccttt ttatgctgaa
    42061 tttatccaga attttagtga agaaaaagat taccaagatg ttttgagaca gtttgaaatc
    42121 agttataaac atctattcga tccaaaaaca aatttgctga ttcatgcatg ggatgaaaag
    42181 aaggtccaac catgggctga tccaaaaaca ggtctatccc atcatttttg gagccgatcg
    42241 attggctggt atttgatggc tgcagcagat acctatgaat tacttcatga agaaacagat
    42301 tgttctttgc tgaaagaaat tctagaaaag acactggctg cattacttcc atatcaagaa
    42361 gaaaacagcg ggacatggta tcaagtaaca acagaaacaa aacggaaagg aaattatctg
    42421 gaagcttccg gcagtagtat gtttgtttac gcaatggcta aaggaatacg tttgggactg
    42481 ctgaacaaag aagaatggct tccacaagtg aagaaatcac atcaaggatt acttgatgaa
    42541 tttgttctgg aaacaaaaga aggctggctg aacttgaata aaaactgtca agtagcaggg
    42601 ttaggcggag cagatcagcg agatggttca tatgcttact acatcagtga accaattatt
    42661 tgtaatgatc aaaaaggcgt tggtgcattc ttgcaagcat tgattgaagt ggaggcatta
    42721 taaatatgta tgatattttg acatttggcg catcaattga tgaattgaat actgaagcaa
    42781 tacaacaagc cattgatgct gcagcaagtg acggaggcgg gaccgtagtt gttccagcag
    42841 gtgaatttct aacaggagct ttatttttaa aaagcaatgt cgaacttcat ttaagcgcag
    42901 gagcggttct gaaattctct gacgatccaa aagattatcc tgttgttcat tcacgttggg
    42961 aaggagtcca tcgaaaagtt tatgcttcct gtatctatgc tcaaaatgta gagaacatct
    43021 ctgtcactgg tttcggtaca ttagatggaa atggaaaaaa atggtggcat acttttagaa
    43081 atgaaccaga taatctcgct tatcctcgtc ctaaattgat gagtttccat aactgtcatc
    43141 gaattactgt gaaagatatt aaattgatcc agtcaccaag ttggacgatc aacccgattc
    43201 tttgcagtaa tgcaacgttt gataacttga cgatcttgaa cccagcggat tcaccaaata
    43261 cagacggaat tgatccagaa tcatgcaaaa atgtgcgtat tagtaattgc catattgacg
    43321 tgggagatga ctgtattgca atcaaggcag ggactgaaga tacgtacgaa aggatcgctt
    43381 gcgaaaatat cacgatcaca aattgcacga tggtccatgg acatggcggt gtcgtattag
    43441 gaagtgaaat gagtggtagt attcgtaata ttaccatttc aaattgtatc ttccaagaaa
    43501 ctgatcgagg aatacgattg aaatctagac gaggtcgagg cgggatcgtt gaagatattc
    43561 gagtatccaa tatagtcatg gataatgtga tgtgtccatt tatactgaat ctttattatt
    43621 tctgtggtcc gcgagggaaa gaaccgtatg tatgggaaaa aaaggcttat ccgatagatg
    43681 aacggacacc agcattcaga aggatccatt tttcgaatat cacggcaagg aatgtccatg
    43741 cttctgcagg ttttatttat ggattagcgg aacaattcat acaagaaatc acatttgaca
    43801 atatcgatgt ctcgatggcc caagatgcag ttcccggtgt tcctgctatg atgacaggaa
    43861 tagaggaaat gagcagacaa ggattttata ttggctttgc gggtaaggtt tcctttaacc
    43921 agatgtcgat tgaaaatcac gagggaccag catttctatt agaaaatact aaagaaatca
    43981 gtattgagaa attccaatca aatcaatcag aaaatatgga tgaactggtt gttcgtaaaa
    44041 actttgctta tgcagttgaa tgatttttta ggtgctccat ctagcagaca tacccctagt
    44101 ggaaaacgat tataccgaga aaaacaaaac ggttttatgc ttgaatacta tcaattgtac
    44161 ttaaatgaat tggaaacagt accagccctc tatgcttatc cagaaaaaga aggaccgttt
    44221 ccgccggtcc tttacctgca ttctcacggc ggagattttt cagtcgggaa aagtgaattg
    44281 atccatggtg ctccttactt agcttctcca tcttttgcag aagtactgac aggaatggga
    44341 tatgctgttt ggtcgattga tgcttgggga tttgaagaac gcggaggtat ttctgaaagc
    44401 gagttgttca aacaattttt attaacagga aaaacgttat ggggaatgcg tatttatgat
    44461 gtgatcagct tgatcgatta tttggagaca agggaagaca cagatactca acggctcgca
    44521 acgattggtc tttctatggg aggcttatta agctggtggg tgacagcatt ggattctcgt
    44581 gtcaaagttt gtattgatct agcggcacag gtggacatcg aaatactttt gctacatcgc
    44641 cgcctagatc atcatgggtt ttattactat gtgccaaatc tattgacagc ttatacaacg
    44701 ttagcaatcc aagaaaaaat cgctccgcgc cctcgtctta gcttagttgg aaaaaacgat
    44761 acgatgtgct tggacgaagg cgtactgaag ctgagaaaag ggctggacaa aaagtatcaa
    44821 tcaatgggta gtccagaaaa cttttcttct ttttctttaa caggcggtca tatggaaaca
    44881 caagaaatga gaaatatttg gaaacaattc ataagggagc atttatgagg atgaaagaca
    44941 aactgataat tggaaagaat gctatagata ccccgacgga ttttgaaact atccaagaag
    45001 gaatcgatta tttggcaggc ctgccggata gccatccaaa ggtcttgata gtaaaagaag
    45061 gaagatatga tgaatatata gaaagcagat tatccaattt taaacttgtg ggagcaggaa
    45121 cagtagttat ctccggtaat cgttttgcaa aacaggagta tcttgacggt agccagttag
    45181 ggactttccg aagtgctact tttttcttag aaggatcgaa tgtcgtatta gaaaatcttc
    45241 acatcgaaaa tacagcaggt ccaggagaaa aggtaggaca ggctgttgct ttattcaatt
    45301 caggagatcg cgtatcttta aaaaactgtc gactatctgg ttatcaggat accttatgca
    45361 cgggtccgtt gcctgatttg caaaaagatg gtactccttt ttgtacaccg gcttctgcca
    45421 atcctgagta ttgtcgtcag acctatgaat attgctggat tgaaggaacg gtggatttca
    45481 tatttggtgg ggcagatgcc gtttttcatc agtgtcatat ccatagctta gccagtaaaa
    45541 atcctggata catcacagca gcatctacgc cgaaaaatca aaaaacaggg tatcgttttg
    45601 aaagctgtac cttgactgca gaacctgata ctagaaacgt ctatttagga agaccgtggc
    45661 gtccgtatgc aaatgtctta tttgaagcgt gtaagatagg tgggcatgtt cacccaactg
    45721 gttgggataa ttgggatgac aagaaaaatg aacagactgt caaattctct gaacgtaata
    45781 acaattatga aacatcgatc aaaagacctg aatggataca tttagaaaag cagggggttc
    45841 agaaatgaaa agaaaatggg aactattgct aggaatggtc ggtggcagtc tatctttgat
    45901 tttctttggc gggttagcag ttactttaag taatatgtct gcgtcagagt ttaaaaaaag
    45961 ttatcagtcg cttgctgtag atcattcaac tttgtcttta gaaaatacat ttggattatt
    46021 acaggacatg actggtctgt ttgccgttgt gttgtttatt tcacttgctt ttttagcagt
    46081 agcattgttt ttgactgcta aaggaaaata cttaacaact gccactggtt tgtattttat
    46141 aacaggattc atattgttga ttggcactca gtttattgcg tttccttttg cattttttta
    46201 tttcgcagct ggggcattca gtttatatcg agtaagaatg agaaaaggag cctgaatcat
    46261 gcagactatc aaagtgaaaa atcctatttt aagaggattc aatccagatc ctaatatatt
    46321 aagagtgaaa gagcggtatt acattattgt ctcctctttt gaatggcttc caggaatccg
    46381 agtatatgag tcgaaaaatt tagtcaactg gtcacataca acggatattt tgaccactca
    46441 agtatctttg caaggaaatc ctacaaatgg aagtatatgg gcgccacaaa tcagttatgc
    46501 tgatggattg ttttatcttg tttacacaga tgtcaaaagt gcaggccgtc cgtttaaaga
    46561 tgtccacaat tatttgatca ctgcatctga gatcacaggt ccttggtccg aaccaattta
    46621 tttaaatagt aacggctttg atccttcttt gttccatgat cttgaaacgg gaaaaaaatg
    46681 gcttgtcaat gaactatggg attatcgtct ggacacaccg aataaatcca gcggtatcgt
    46741 attacaagaa tatgatgcag agaagaagaa gcttgatggt cccatctata aaatatttgc
    46801 aggtacagaa ttagcgaaaa cggaggcacc acatttgtat tttcgtaacg ggtattatta
    46861 tttgatgacg gcagaaggcg gtacaggaag aggacattca gttacagttt gccgagcaaa
    46921 gaatattact ggtccttacg agcttgatcc tggttttcct atgctgacag ctagtgacaa
    46981 acctgcttct acattgcaat gtacaggaca tgggagctta gtacaaaccc cgtcaggaag
    47041 atggtacatg acatatcttt gtaccagacc attaaacggt gcggctattt tgggaagaga
    47101 aaccgctatt caagaagtgt attggacgtc cgacgattgg ttaagactta aagatagcca
    47161 gacaactccg atggaagaaa taatgattga gacaaatgaa gaggtacaac aagaaaaaga
    47221 tcatcagttt atggatacat ttgaaggaga gctaaagaag aactggaatg ccagacgcat
    47281 tttgccaaat gctgattggt gtgatacaaa atgtagacca ggttttttac gtctgatttc
    47341 cggtgaatct cttcaatcta ccttcgaaca tcatttgtta gctattcggc aaacggattt
    47401 tacttttgat gcggagacag catttgaata catgcctaat aattttaatc agatggctgg
    47461 actggtactg tacttaaatg aatccaatta tatttattgt tatgttactt ggaatgagga
    47521 caagggaaaa tgcctacgta tgatagaatc agaaaacgga gtagcacaaa tagaagattg
    47581 gacgattcca ctcacagaaa agaagacttt tttgaaagtc actgtaaaga aagagacagc
    47641 acagtttcat ttgaaagacg tatcaactga agaatggaag aaagtaggtc cgcaaaaaaa
    47701 catgtgtata ctctcaggag ggtttactgg aaattttatc gggatagcag tgcatgatct
    47761 aaataaaaaa agaggaagtt atgctgattt tgagtttttc aagtatgatg gaaaagatca
    47821 tctgtaattt aattctttaa taaatgaggt tcactaactt attttcacta gttagtgaat
    47881 ttttttgttt gtatatattg ttatgtaaaa aaagttatca atcttatgca tcaatgtttt
    47941 tagattattt tttgtttttt atctgtcaag ataatgcaat aaaaagccaa cattgtgaat
    48001 ataaaaacaa gaaaaaaaga gttataattt tgaaaacgtt tactagaaga ggtaggagga
    48061 attcaaatgc tttcacaaaa tgctgttaca gcaaatacaa taaaaaaaga aaaaaaggaa
    48121 tttgggattt ccgataagat aggctacatg ttcggtgatc taggtaactg ttttatttta
    48181 ggtttagtca atagtttctt gatgatttat tatacgaatg ttcttggcgt ttcaggggca
    48241 atcgtaggta cgttattctt gatttcgaga attttggatg cgtttgctga tgtgatggtt
    48301 ggaagattat gtgatgtctc agagttaaca gaaactggcc gatttattcc ttggatcaga
    48361 aaaatgaagt atccattttg cttagtgact gtcattttgt ttttgccatt cgtccaacat
    48421 cttccaatga cggtgaagat tgcttatatt tttgtttcat acattgttta tgggattttt
    48481 ctctcaacaa tcaatatccc gtatggagca atggcagcag caattagctc aaatcctaat
    48541 gaccgcacct cgttgtccac gttccgaagc attggttctg cgattggcgg atcaactaca
    48601 ggattcttca ttccgatttt aatgtatacc cataacaaga gtggacaaca agtcgtttca
    48661 gggaatcatt tcttttggat ttcaatcgga tgtgctgcaa tagcatttgt cgcatatatg
    48721 ttaacttgta aattgactac tgaaagagta agagttgtca aaaacgaaaa agtatctgct
    48781 tctcagttgg taaaaggttt gttagctaat cgagcactga ttattttagt cattgttgac
    48841 attttcattg tgatcaatca gattttagct gggacgaata tgacttattt attcaatgat
    48901 tacttccata ataaacaagc aatgtctgtt gctcttttat ttacttacgg aacagtagta
    48961 gttttagcac catttgctac aaagctgaca aaattatttg gtaaaaagga agcaagcgta
    49021 tttgcgttac ttgcttcttc atgtatgtac ttgctgatgt atttccttca tataacaaat
    49081 gcatgggtat atctgattct tttatttatc gctactttag gatctggttt attcaatttg
    49141 atggtctggg cattcattac tgacgtgatt gattatcatc aatataagac aggcttaaga
    49201 gaagatggaa cagtatatgg agtaaattct tttgcgagaa aagtaggaca agcatgtgca
    49261 ggagcggtag gcggatttat gctggcactc attggttacc aatcgtctac aacaggtgga
    49321 gccatccaat cagttttagt acaagaaagg atttatacga tggcaaactt gttaccagca
    49381 ttctgcttat ttgtatccgc aatcattttg ctagtaggtt acccactgaa tcgttcaaaa
    49441 actgtattta ttggtgaaga attgaaaaaa atcaatgaaa gatagggaga aacgtattgc
    49501 ggacaaaaaa agggattatc aatgctttga actttaaaac aaacgacact caacaagatg
    49561 atacaataat actgcaaaaa gcaatcgatc aaggtgcgaa agaacactta cctgtattta
    49621 ttccaaaagg catttattta gtcggagccc tttttttaaa agataagagt catcttatct
    49681 ttgaagaagg tgcagtcctc aaggggagaa cagatatcga agcatttcca gaaatcgata
    49741 cacgagtagc cggcgtagaa atgaaatggc cagcagcgat tttgaatgtt ctctcggcga
    49801 aagatattct tatagaagga aaaggaataa tcgacggtca aggagaccat tggtgggagc
    49861 tgtattgggg aaaagatcaa aaaagcggga caagagctga atacgatcaa aagggacttc
    49921 gttggattgc ggattatgct attaaacgtc cacgcgcttg tttactgtat catgcagaac
    49981 atgttgttgt tcgtgatttg atttttcaga aatcaggttt ttggaacttg caaatcactt
    50041 attctaacga tgtattagta gaaaaagtaa tcatccgcaa caatgatgga ccaagtacag
    50101 atgggattga tattgattct tcaacaaatg tgcgtgtcta tgaatgtgat ttggcctgtg
    50161 gtgatgattg tattgctatt aagtctggca gagatggcaa tggtgcaaga gtcaatcgaa
    50221 aatcatcaag aatcgaggtt gctagatgta aaatccgttc aggatacggt gtaactattg
    50281 gtagcgaagt tagtgctgga gttagtgacg tatatattca cgatatcgat ttttttcagt
    50341 cagactgtgg tattcgaatg aaatcttcaa aagaaagagg gggagtgatc gaaaatatca
    50401 gggtagaaaa tctgaatatg attgatgtac aatttccttt ttcttggata atggactggc
    50461 ataatgaata caatagaaaa aatagcaatg accttgctca aatgccagaa agttggcaag
    50521 cagtggctga agaaatacct gaatcaaagc aaatgacgaa agtcaaagac atacacatca
    50581 aaaacgtaca ggcatgcctt tctccaggtt actcacttcc ttcccgagct tttgatttag
    50641 tagcatttcc tgaaaagcct atagaagatg tctgcttttc tcattgccaa ataactgcaa
    50701 aagaattcgg gcgaatcgaa gcagtacaaa acttatgttt caaaagctgt gatttatcta
    50761 tagaaactgg caacacagct gcaaacaata cttttgacaa tcgttgaaat tataataagg
    50821 tcaagcttaa ataatatttt agcttgacct ttattttttt taatgatata ttaaacaacg
    50881 ttgtcgcgct tcattgaaac attttatgaa aattccaaaa aagagttgac acatgttttt
    50941 agacgtgata agataactaa gtcgt
//
LOCUS       DANJMI010000019        48796 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000019 DANJMI010000000
VERSION     DANJMI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.019"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(285..770)
                     /locus_tag="PWU81_001279"
     CDS             complement(285..770)
                     /locus_tag="PWU81_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL1109993.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    860..1205
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1250..1891
                     /locus_tag="PWU81_001280"
     CDS             1250..1891
                     /locus_tag="PWU81_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL1109994.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1910..2545
                     /locus_tag="PWU81_001281"
     CDS             1910..2545
                     /locus_tag="PWU81_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL1109995.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2563..3378
                     /locus_tag="PWU81_001282"
     CDS             2563..3378
                     /locus_tag="PWU81_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1109996.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3697..5133
                     /locus_tag="PWU81_001283"
     CDS             3697..5133
                     /locus_tag="PWU81_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL1109997.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5322..7754
                     /locus_tag="PWU81_001284"
     CDS             5322..7754
                     /locus_tag="PWU81_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL1109998.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            7984..9051
                     /gene="rlmN"
                     /locus_tag="PWU81_001285"
     CDS             7984..9051
                     /gene="rlmN"
                     /locus_tag="PWU81_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL1109999.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9244..10347)
                     /locus_tag="PWU81_001286"
     CDS             complement(9244..10347)
                     /locus_tag="PWU81_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL1110000.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10899..11447
                     /gene="aac(6')"
                     /locus_tag="PWU81_001287"
     CDS             10899..11447
                     /gene="aac(6')"
                     /locus_tag="PWU81_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL1110001.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            11993..13654
                     /locus_tag="PWU81_001288"
     CDS             11993..13654
                     /locus_tag="PWU81_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1110002.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(13724..14086)
                     /locus_tag="PWU81_001289"
     CDS             complement(13724..14086)
                     /locus_tag="PWU81_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDL1110003.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            14154..15095
                     /locus_tag="PWU81_001290"
     CDS             14154..15095
                     /locus_tag="PWU81_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1110004.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            15096..16139
                     /locus_tag="PWU81_001291"
     CDS             15096..16139
                     /locus_tag="PWU81_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1110005.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            16145..17215
                     /locus_tag="PWU81_001292"
     CDS             16145..17215
                     /locus_tag="PWU81_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1110006.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            17205..18158
                     /locus_tag="PWU81_001293"
     CDS             17205..18158
                     /locus_tag="PWU81_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1110007.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            18170..19180
                     /locus_tag="PWU81_001294"
     CDS             18170..19180
                     /locus_tag="PWU81_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1110008.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(19465..20286)
                     /locus_tag="PWU81_001295"
     CDS             complement(19465..20286)
                     /locus_tag="PWU81_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL1110009.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(20383..20661)
                     /locus_tag="PWU81_001296"
     CDS             complement(20383..20661)
                     /locus_tag="PWU81_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110010.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            20929..22938
                     /gene="metG"
                     /locus_tag="PWU81_001297"
     CDS             20929..22938
                     /gene="metG"
                     /locus_tag="PWU81_001297"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL1110011.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(23417..24004)
                     /locus_tag="PWU81_001298"
     CDS             complement(23417..24004)
                     /locus_tag="PWU81_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110012.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            24179..25501
                     /locus_tag="PWU81_001299"
     CDS             24179..25501
                     /locus_tag="PWU81_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1110013.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            25656..26432
                     /locus_tag="PWU81_001300"
     CDS             25656..26432
                     /locus_tag="PWU81_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL1110014.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            26425..26994
                     /gene="rnmV"
                     /locus_tag="PWU81_001301"
     CDS             26425..26994
                     /gene="rnmV"
                     /locus_tag="PWU81_001301"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDL1110015.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            26987..27871
                     /gene="rsmA"
                     /locus_tag="PWU81_001302"
     CDS             26987..27871
                     /gene="rsmA"
                     /locus_tag="PWU81_001302"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL1110016.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            28322..28951
                     /locus_tag="PWU81_001303"
     CDS             28322..28951
                     /locus_tag="PWU81_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110017.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(29075..30184)
                     /gene="ugpC"
                     /locus_tag="PWU81_001304"
     CDS             complement(29075..30184)
                     /gene="ugpC"
                     /locus_tag="PWU81_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDL1110018.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            30393..30815
                     /gene="mgsA"
                     /locus_tag="PWU81_001305"
     CDS             30393..30815
                     /gene="mgsA"
                     /locus_tag="PWU81_001305"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL1110019.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            31138..31674
                     /locus_tag="PWU81_001306"
     CDS             31138..31674
                     /locus_tag="PWU81_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL1110020.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(31714..33477)
                     /locus_tag="PWU81_001307"
     CDS             complement(31714..33477)
                     /locus_tag="PWU81_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1110021.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(33480..35195)
                     /locus_tag="PWU81_001308"
     CDS             complement(33480..35195)
                     /locus_tag="PWU81_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1110022.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(35974..36198)
                     /locus_tag="PWU81_001309"
     CDS             complement(35974..36198)
                     /locus_tag="PWU81_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110023.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            36389..39166
                     /locus_tag="PWU81_001310"
     CDS             36389..39166
                     /locus_tag="PWU81_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL1110024.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            39247..39666
                     /locus_tag="PWU81_001311"
     CDS             39247..39666
                     /locus_tag="PWU81_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDL1110025.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            39678..40148
                     /locus_tag="PWU81_001312"
     CDS             39678..40148
                     /locus_tag="PWU81_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1110026.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            40165..40944
                     /locus_tag="PWU81_001313"
     CDS             40165..40944
                     /locus_tag="PWU81_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1110027.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            40931..41752
                     /locus_tag="PWU81_001314"
     CDS             40931..41752
                     /locus_tag="PWU81_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL1110028.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            41769..42818
                     /locus_tag="PWU81_001315"
     CDS             41769..42818
                     /locus_tag="PWU81_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL1110029.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            42831..43835
                     /locus_tag="PWU81_001316"
     CDS             42831..43835
                     /locus_tag="PWU81_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL1110030.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            44468..45487
                     /locus_tag="PWU81_001317"
     CDS             44468..45487
                     /locus_tag="PWU81_001317"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL1110031.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            45530..46411
                     /gene="galU"
                     /locus_tag="PWU81_001318"
     CDS             45530..46411
                     /gene="galU"
                     /locus_tag="PWU81_001318"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL1110032.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            46711..47088
                     /locus_tag="PWU81_001319"
     CDS             46711..47088
                     /locus_tag="PWU81_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110033.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            47694..48308
                     /locus_tag="PWU81_001320"
     CDS             47694..48308
                     /locus_tag="PWU81_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL1110034.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT        16034 a         8458 c        10094 g        14210 t
ORIGIN      
        1 taaaaaagtt tagttagaat ataatatttt ttgttaaata tgactgaaat caaaagaata
       61 aaattccgat ttataatcga aaaaagaagc atttttatat atgcttcttt tttcgtagaa
      121 tcaataaata tggatttggc taactatgga agaatactcg tattagtttt cagcgttcat
      181 actagttgtt acattgtttg attggccaaa agaatttctg atatattttg tgctaaataa
      241 agaaaaagac gcttgaattg ggaaataaga aaactactct atcattacaa attttcataa
      301 actggcagat gttctaagta aacaagtatc ttttcaaagt cggaaaaatc cataatcaac
      361 gtagtggtat tgatatttgg atgaaagcct acggtatctt gacgatcaat agcagcatcg
      421 atcacgactt ggattttttt ctctttgtcg tgcatcaatg ctaatggagt cactgtacct
      481 gctggaacgc ctaggaggtc aggaagctgt tcttcagaaa caaaagataa gcgcttttct
      541 tttaattgtt cagctagttg ttttaaatcg gcgtgctttt catcaggcaa gatgacaaga
      601 tagatttgtt ttccttttct tgatttcaat aatagatttt ttacttgcgg accaggtagg
      661 gaaaaaggga catttttgac tgacgtaatc gcaggatgat cgactttttg aaaagagata
      721 cccagttttt ttagtagttc ataagcttct tgttcggttg cacgactcat cgcgcatcac
      781 cctccttttt ttattagtgt atcataaaaa agagttgaca aatgaaaaat aatcatgtaa
      841 attacacgta aagttaaaac aaatgacatc gaaaagaaga gtagccgtta ggtgttttta
      901 gagagtctct ggttggtgga aagaggcagc agttaaccgt gaaacacatc ttggagtcga
      961 ttttctgaag cgtatagtag gaaaatccgg ggagcccgtt acagctgcgg tcgttgctag
     1021 atgaccagat aaggctggct tttccgtgag gagtcggtaa aaaaaggtgg aaccacgtga
     1081 ttaacgtcct ttgatcttcg gatcaaagga ctttttgttt tcatctgaac ggcaacgact
     1141 gtgtgaggtt ttttctgcaa ggaaaaaata aacagaggtg gaaccacgta taatacgtcc
     1201 tcttggcatt tgccaagagg atttttttat tttaaaggag gaaaagaaga tggagtattt
     1261 gattgaagtt atgccgcaat tattagtagg agcactcaca acattgaaat tatttttatt
     1321 taccttgatt ggctcattac cattagggat tgttttagca ttaggattgt ctttgcattt
     1381 taagccgcta cgctacttgc ttcaaacata tgtatgggtt atgcggggaa caccgctgtt
     1441 gttgcaactg atttttattt tttatgggtt gccgacgatt gggatcgttt ttgatcgttt
     1501 cgaagcagca ctcattgcct ttatcttaaa ctatgcagct tattttgcag agatctttcg
     1561 tggagggata caatcgattc ctcaaggtca atatgaagca gcgcaagttt tgcaattaag
     1621 tccctttcaa acggtgacga aaatcgtcat cccacaagtg acaaagattg ttttaccttc
     1681 tgtagggaat gaagttatca acttggtcaa agattcttcc ttgatctata tattaggttt
     1741 aggtgacgta atgcgtgctg ggaaaatcgc gatggaacgc gacgcgactt tactgccttt
     1801 gctaggcgtg gcaatcatct atcttacatt gactggtatt ctaacaatcg taatgaaaca
     1861 agttgaagga aaattaaatt attaccgcta actgatagga ggaagaaata tgattgaatt
     1921 gaagaaagtg aacaaagcat ttggtgaaaa aaaaataatc aaagatttga atctaatcat
     1981 tccagatggg aaaacattag ctatcgtagg tccttccggt ggcggtaaaa ccacgctttt
     2041 acgtatcttg gctggcttag aaagttctga cagcggagaa ttttatttag acggtgtacc
     2101 gttcaaccca ttcgagctta aaatgaaaga acaagttgta ggagtcgtat tccaagactt
     2161 ccagctattt ccacatctga atatttttga aaacattacc ttagcaccaa aaatggtatt
     2221 gaaacagaca aaagaagaat acaccaaaaa agcagaagaa ttagcggctt cattagggat
     2281 tagtgattta ttgaatatct atccttatca gctttctggc ggacaaaaac agcgggtagc
     2341 gattgccaga gcattggcta tgaatccacg ggtattggct tatgatgaac caacaagtgc
     2401 tttagatcct gaattacgcc agcaagtcga gcgtttgatc ctatcattga aagaagatgg
     2461 ggtaacccaa attattgtca cccatgattt agtatttgca gaaaatgtcg gtgatcaatt
     2521 actaaaagtt tccccaattc attaagaaag gagaagatag agatgaaaaa aaccagtttt
     2581 ctattcgctt tgatcgcagg tcttcttctt tttgcaggct gttcgaacaa aaaaacttca
     2641 gcagataatt gggcgactat caacgaagag aaaaaaatcg ttataggttt agacgataca
     2701 tttgttccta tgggatttag agataaagac gggaagctca caggatttga tattgattta
     2761 gccaaagcag tctttgaaca atatgggatt acggtagact ttcagcccat tgactggtca
     2821 atgaaggaat ttgaactgaa caatggaacg atcgatttga tatggaacgg ctattccaaa
     2881 acgccggcaa gagaaaagaa agtgcaattt acaaaacctt atatggaaaa cgatcaagtg
     2941 ctgatcacac ctaaagaatc aggaatcaca aactttggac aaatgaataa taagagatta
     3001 ggtgcgcaaa atgggtcatc tggttatgat gtatttacga aacaaccaga agtattaaaa
     3061 gagatagtca aaaaccaaga tgctgttttg tatgatagtt ttaatgaagc attgatcgat
     3121 ctgaaaagca gacgggtcga tggtctgctg atggataaag tatacgcaga ctattatttg
     3181 aaacaaagaa acgaactaga agacttcaac ataacaaaag gttcttatga gagtgaggat
     3241 tttgcagttg gtgcacgcaa gtcagatagt acattagtga aaaaaatcaa tgaagcccta
     3301 gatcaactag agaaaacagg tgaattccaa aaaatatccg ataaatggtt tggcgaagat
     3361 gttacgccga gaaattaaca aataggggac tgtgacaaaa gacgtgtcac agtccctttt
     3421 tccgaataaa cagtgttctc ctgccaactc cttgtgactc ggagctgaat ggcaggctca
     3481 caacctcttg gtaaacggtg ttcaaaagcc agtttttcgg tattaagcca aatttatgca
     3541 aaaatgtaga aagccatttt cacaaaattt ttcttactac tcgaaactaa gcgacttttt
     3601 cacaacctcc tttacataat tttattttgt tgtggaaggg gtttcgtgtt attctaaaag
     3661 aggaacgatt gaaggttata ggaaaggaag agatgaatgg aacacaaaga acttacctca
     3721 tttccaaaag attttctgtg gggctctgct tctgcagcgt atcaagtaga aggagcttgg
     3781 caagaagatg ggaaaggaga atcagtctgg gatcgatttg tacggatacc tggaaagaca
     3841 tttaaaggaa cgaacggtga tttggcagta gaccactatc atcgttataa agaagatatt
     3901 gctttaatga aagaacaagg tctgaaagct taccgcttct ctgtagcttg gacacggatt
     3961 tttcctaatg gacgaggaga agtcaatcaa gctgggctcg ctttttatga gagattgatc
     4021 gacgaattga ttgaaaacga tattgaacct atgttgactt tatatcattg ggatcttcca
     4081 caagcattgc aagatgaata taatggttgg gaatcacgtc agatcattga agatttcact
     4141 cattatgcag aaacattatt tgaggcattc cgcgggaaag tccattactg gattagtttg
     4201 aatgaacaaa atatcttcac ttcattaggt tatttgttag cagcacatcc gccaggtgtg
     4261 acagacccga aacgaatgta tgaagttaac catattgcta atttagcaaa tgcatctgtc
     4321 atcaataaat ttcacgagtt agaaatgcct ggaaaaatcg gaccaagctt tgcgtatacg
     4381 ccaaattatc caattgacag taatcctgaa aatgttctgg ctgctgaaaa tgcagaagac
     4441 ctgatggcca attattggat ggatgtctat atgtggggaa aatatccgat tgctgccatg
     4501 aggttcttag aagaaaaagg atgggcacca acgattgaag ctggcgatgc agaattgcta
     4561 gaatctgcta aaccagattt tcttgggatc aattactatc aaacggcgac gaatgcattc
     4621 aatccattaa acggagttgg tgctggaaaa atgaatacga caggaaaaaa aagttcatca
     4681 gaagaaactg gagttcctgg aatgtataaa aaagttgaga atccatttgt cgaacgaaca
     4741 aattgggact gggagattga cccagaagga ttaaggatcg gactgcgtcg tatcacaagc
     4801 cgttaccgca tgccggtact gattacggaa aatggcttag gagagtatga caagttgaca
     4861 gaagataaac aaatccatga cgactatcga atcaattact tacaaagcca cgtcaaagcc
     4921 atcaaagaag caatttcgga tggagcagaa gtcctaggct attgcacctg gtcttataca
     4981 gatttgctta gctggctgaa cggttatcaa aagcgatatg gatttgtata tgttgatcaa
     5041 gacgaaacac aagaaggatc tttggaacga tataagaaag acagttatta ctggtaccaa
     5101 aaactgattg aagaaaatgg agagaacctt tagttcacat acttaaaaca agaaggtacg
     5161 gctggatatg gccgtgcctt ttcttgataa aatcatttag aaattaagaa aaactgtaaa
     5221 ataagctgaa aaatgaaaga aagcgtcagt tctttcaaac aaaactttag ttcccttact
     5281 tttttttgta taatgaaact tgatgaaatg agggataata aatggcacaa aagaaacggc
     5341 ctactaaaaa aaggaaaaag acgaaaaaaa cgaaaaagca aaaacagcag caggaacaaa
     5401 tgctgacgat tggtgttggg tttgccttga ttttatttgc tgtcttcggc tttttaaaat
     5461 taggtttttt aggaatattg attgcaaacg gctttcggat cattgcagga aatacttacc
     5521 aaatcctctg tcttttatta gccgtattag gattttggat tgtcattaag aatacagagt
     5581 tttcaattgg taaaaaccga cgctggtttg gcggcatact cttttatttc ggtatcttat
     5641 tattgttaca tgcccattta tttggcaaac tccatactgg ggaaccaaat atcatgggga
     5701 cgacatggga tttgttagcc agtgatataa aacaaagtca agtagacaat aacgttggtg
     5761 gtgggatgat tggagcaatc ctttatcatt ttacttactt cttgattgca cagcctggaa
     5821 gttatttagc agcagtgcta ttactggcag gaggagcatt cctgttcagt aatctggaag
     5881 gctatcagct gttaaatggt atccaatcaa ttggagaacg agtacaggaa ttattggaag
     5941 gtgatcctgc caagcaagct cgaaagcaag cggcaaagga agagagaatg aaacagcgcg
     6001 cagaagcgaa agaagcaagg cgcttagctg caaaagaagc tgcagaatat gagaagaaaa
     6061 aagcggtgag ccagcaaagg gaaaatactg ctgatgaatg gcaggaacca gaaaatacag
     6121 agccggagca gctaagtttt gttccaatcg acagttttca agaaaatatc catcctgcga
     6181 atctagagaa accagtacca gatacaccaa aacagaccaa tacagcagaa ggtttttcag
     6241 atgagttgcc agaggatgac ggtacctcat tagaatttga gattgaagca gaacaggaaa
     6301 atcaagacta tgaattaccg acagttgatt tgcttgattc tataccaact gtggatcaaa
     6361 gtgatgagta taaaaaaatt gaaaaaaata tcggcgtatt agaacaaact ttccaaagtt
     6421 tcggtgtcga tgccaaggtc gtcaaagcca gcttaggacc atccgtcaca aaattcgaag
     6481 tccagccggc tgtaggggta aaagtaagta aaattgtgaa cttaacagat gatatcgcgc
     6541 ttgctttagc ggcgaaagat gtccggatgg aagcaccgat tccaggtaaa tcactgattg
     6601 gtattgaagt accaaatgga aagatcagta tggtttcctt tagagaaatc atcgaagcac
     6661 aaccaaacca cccagataaa ttactagaag tacctttagg acgtgatgtt tctggacggg
     6721 tacaaacggc tgatctttcg aagatgcctc acttactcgt ggctggttcg actggtagtg
     6781 ggaagtcagt agcaattaat gggattatca caagtatatt aatgcgtgct aagccccatg
     6841 aagtaaaact gatgatgatc gatccgaaaa tggttgaatt gaatatgtac aacggcatac
     6901 ctcatctgtt gacgccggtc gtgacgaacc ctagaaaagc agcgcaagcc ttacaaaaag
     6961 tagtacaaga aatggaagaa cgatatgaaa aattcgcggc aaccggagta agaaatattt
     7021 ctggttacaa tgaatttgtt caacagaaaa acttggaaaa cggaacgaaa catccaacct
     7081 taccattcat cgtagtgatc gtagatgaat tagctgattt gatgatggta gccagcaatg
     7141 aagttgaaga tgcgattatt cgtttagcgc aaatggctcg ggcagcgggg attcatatga
     7201 tcctagctac tcaacgtcca agtgtggatg ttatcacagg aatcatcaaa gcaaatgtac
     7261 cttccagaat ggcatttgcg gtatcaagcg gtacagattc tcggacgatc attgattcta
     7321 atggggcaga aaagctctta gggcgaggag atatgctatt cttgccaatg ggtgagaata
     7381 aaccgatccg tgtgcaagga gcattcattt ctgaccacga agtagaacgg gtcgttcaat
     7441 ttgttacaga tcagcaagaa gctcattatg aagagaaaat gatgccgaca gatgaagttg
     7501 aaacgacagg tgctccagaa cagccacaag atgagctgtt tgaagaagca aaagcacttg
     7561 tcgttgaaat gcaaacggca agtatttccc ttctccaaag aagatttcgg atcggttaca
     7621 accgagcagc acgtttagta gatgaactgg aagctcatgg agttgttggc ccgtcagaag
     7681 gaagcaagcc tcggaaagta ttcattgagc aggaagaaac atcagaaacg ttgccggacg
     7741 ataatgacat gtaaaacttg tgtgatgaag ggttgaaaaa cgaataacca ataaaaagac
     7801 aaggcatgat gataccaaaa gaaaaaatgg tttttttcca tcttttctaa tacggtatga
     7861 tgtcttgtct ttttagtttt tgtgagatta aagttctatt cgatttattt gtgaagtatt
     7921 gcattaagga aatgaatcat gtatagtatg tagcgaaact ttgagaaaaa gagggaaaat
     7981 actttggaaa aacagtccat ttatggcttg acgaatgaag aattaatcaa ttggtttata
     8041 gaaaacggcg agaaaaaatt ccgagctgcc caagtttggg aatggttgta tcaaaaacgt
     8101 gtaagtaatt tcacagaaat gacgaatcta tcgaaacaat tgatcgaaaa attatcggag
     8161 cattttatca tcaatcctct aaaacagatg gtcgtccaag aagcttcgga tggtacagta
     8221 aaatatttgt ttgaactccc tgataaaaac atgattgaga ctgtactgat gagacaagaa
     8281 tatggcttgt cagtttgtgt gacgacacaa gtaggatgta atatcggctg tacgttttgt
     8341 gcaagcgggc tgctcaaaaa gaaccgtgat ctgactgctg gagaaattgt tgcacagatc
     8401 atgatggtcc aacattattt tgatgagcgc aatcttggcg aacgcgtttc acatgtcgtt
     8461 gtaatgggga tcggtgagcc atttgataat tacgacaatg taatggactt tttgcatatc
     8521 atcaatgatg ccaaaggatt ggcaattggt gctcggcaca ttacagtttc cactagtgga
     8581 ttagcacaca aaatcaagga attcgctaat aacggcttgc aagtcaatct ggctatttcc
     8641 ttgcacgcgc cgaataatga ggtgcgaaca tcgatcatgc ggatcaaccg aagtttccca
     8701 attgagaaat tgatggaagc tgtggatgag tatttagaga aaacaaaccg tcgaatcacg
     8761 tttgaatata tcatgttgaa tcaagtaaat gatagaccag agcatgcgca acaattagca
     8821 gatctattaa aagataagaa aaaattagca tatgtcaact tgattccata taatccagtc
     8881 agtgaacatg atcaatattc tcgcagtcca aaagctgatg tattgagatt ttatgatgta
     8941 ttgaagaaaa acggcgtaaa ctgtgtgata cgtaaagaac atggtacaga tattgatgca
     9001 gcttgcggac aattgcgaag caagcaaatg aaaaaaacag ttgccaaata atcgtatcct
     9061 actcgttttt tcaattacca taaaaggagc agttttctta attaaggaac tgttcaactt
     9121 aaataagaga aaagattcta tagcgaaagc tataaagctt tttttgacaa gaaaaaccct
     9181 catcaagcaa agaaatgtcc tcaatttgat gacagcctgc ttgatggggg ttttagcaat
     9241 tatttaactg attttcttat atccttgctg gctttcttgt gtagcatgat aagtttctac
     9301 gtcaagaatg gcctgtttaa atttttctcg ggtgatttca taaggcacat gtttaatatc
     9361 atttgttgcc atggctttat ctaagattgc ttctaaatcg gtcgtgacat tgagattcat
     9421 gtcggttaac gatactggaa gttggttctt tttcataaac acatagattt tatcaaacaa
     9481 atcgaactgc tgatccatca tcaataaggt cagtacacca taactgacag atgctccgtg
     9541 aaggtatccc tccatttttt ctagaacagt cgtaccgtaa tagaatgagt gtgccatagc
     9601 agaattatag ttattgttaa ctaaaacaga aaccaatccg gtagtaacaa tgatatccga
     9661 aacgacttgt tcaattgcat atgagggacg atcgtcggca ctatctttca atgcttgcgt
     9721 accataagtc aagagacgat caatgcacat ttttccgatt tggattccta atctatccgt
     9781 gtagcctaga tcatcgccac gagcagaaaa agtagattcg acttctttgg ataaagcatc
     9841 gccaatacct gcccataagt atttatcagg tgcttcggca ataattgctg tatcaatgaa
     9901 ggtgtgtaca ggtggttttg aaggaaagaa gagtccgtcc attacgcctt cttcgttata
     9961 gatgacacat accgcagtga cgggcgaaca gttcgaacca atagtgggaa aggtgaagac
    10021 tggtttagct agtttttcac caatgacttt tacagtatcg caagcacggc caccaccgac
    10081 tgcaaacagg acgtctgctt tttggacact ttcatgctgg atcaactgtt caatatttcc
    10141 ataagttgaa ttacccccgt accagagact ggcgacaacc tctatttcct gtgtcaaagc
    10201 tacttttatt ttgttttctg cctttgaaag tgcagtcttc cctccaatca gcactacttt
    10261 tgtcccatat tctttgacga ttccatttgc tttttcatac gcatctgttc caatggtata
    10321 atttggtaag aaaacacttt gactcatatt catttccttc tttcttcgtt gaaacgggtt
    10381 aaaattttaa agagtgatcg atctgactgc ggctgtagat ggagtagagg tgaacctaag
    10441 acaggataag tgatgaataa ggtggcatga acaccttgaa atcatcaata agtatcctaa
    10501 aaacgaacaa gtacaaaaac attgaaaacg acaataatga atttttgttc ctcttagtat
    10561 ttctaccgat tccctttgct tttttgtaat tatctaaaaa ttctaaaaat taagattatt
    10621 atatgaaaac aggttttatt tgtcaattca taattggttt tttttaaagc aaaagataat
    10681 ctatatcatg atgaacaaac agagtttttt cctccaattg atggctttcc tgatctttcc
    10741 tattttgaat aaagtgtttc tgtataattt tttattttat aagtaagata aataatgaaa
    10801 gaatagagat actgattggt agctaaagat ttttgagcga gaacatttcg ttttttcaaa
    10861 aggactgtta gaataagcaa gaacaagggg gaagacgtat gataatcagt gaatttgacc
    10921 gtaataatcc agtattgaaa gatcagcttt ctgatttact gagactgact tggccggaag
    10981 aatatggaga cagctcggca gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg
    11041 tagcagcggt agaccaagat gagttagtag gatttattgg tgcaatccct caatacggta
    11101 tcacaggttg ggaattgcat ccattagttg tagaaagctc ccgacgaaag aaccaaatag
    11161 gtactcgatt agtcaattac ttagaaaaag aagtagcttc cagaggagga atcacgattt
    11221 atttaggtac ggatgattta gaccatggaa caacgttaag tcaaacggac ctgtatgagc
    11281 atacatttga taaagtggct tccatccaga accttcgtga acatccgtat gaattctatg
    11341 aaaaattagg ttataaaatc gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg
    11401 atatttggat ggcaaaaacg attattcctc gaccaaattc tcaataataa aaagaggttg
    11461 tgacagaagt aatcagcttc aagaaataag aaggaattta cgaaaattac ttcttgaatt
    11521 tttgtgaaat tccaacttat ttccgaagaa gctacttttg gaacaccgtg taccagaggt
    11581 tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg gctttctaca
    11641 tttttgcata aatttggctt aataccgaga aactggcttt tgaacaccgt ttattcagat
    11701 aagggactgt gacaagactt ttgtcacagt ttctttttgt ttattctttt ttatctttgt
    11761 atggcaataa tagacaatat aacgaaaatt atttaatggc ttttggaaga ccatggaaaa
    11821 ccttgtacaa agctattttt tctgaaaaat tccttgaaac ctcacgaaaa aatcattatt
    11881 ttatgaaaaa gacaaaaaaa agtgttgatt ttttaataat tattcattaa actcttaaac
    11941 aacttcagaa aatattaatt attctgaaaa ttccaataaa ggggtacgta aaatgaaaaa
    12001 gaaatttaca tttggtttgg ttgcggtatg cggccttgta cttgctggat gttatggcgg
    12061 cagttcagcc acaagcagta gttcagcaaa aagcggttca gcagatggag gaggcgtatt
    12121 taacttagtc gtgcctcaag aaatgcctac agctgactta tcagttgcaa cagatacaat
    12181 cagttttaca gcattgaata acgtttatga aggaatttac cgtcttgatg aagacagcaa
    12241 accacagccg gcaggagcta gcgagttggc agaagtcagt gaagacggat taacttacaa
    12301 aatcaaatta cgagaagacg caaaatggtc aaatggtgat cctgttacag cagcggatta
    12361 cgtttatggc tggcaacgaa cagtagaccc agctacagct tctgaatatg cttacctgta
    12421 tgcgccagtt gaaaatgcag aagacattac agctggaaag aaagataaat cagaactagg
    12481 gatcaaagca gttggtgact atgaattaga aatcaaattg acgaaacaaa caccatattt
    12541 ccaatatctg cttgctttcc catcattttt cccacaaaaa caatccgtag tggaagaaaa
    12601 tggggatgcc tatgcatctg caagtgataa agctgtttat aatggaccat tcacattaga
    12661 agattttgat ggaccaggaa cagatacaga atggacttat aagaaaaatg atcattactg
    12721 ggacaaagat acagtaaaac tttctgaaat caaagtcagt gtagtaaaag aatcttctac
    12781 tgcattgaac ttattcaaag atggccaagc agatgacgtg atcttgtctg gtgagttagc
    12841 acagcaaaac gcaaacgatc cagcttatac ttctgttaaa gaagcgcgga caagctatat
    12901 cgaattcaat caacgtgaaa agaattcacc attcaacaat gtgaacctaa gaaaagctat
    12961 ttcttattct atcgatcgtg aagctttagt aaaacaagta ctaggtgacg gatcagttgt
    13021 ctctacagga ttgatcccat cagatatggt aaaaagtcca acaacgaacg aagactttgc
    13081 aaaagaagct ggaaaattag tttcttatga caaagataaa gcaaaagaat attgggaaaa
    13141 agcgaaaaaa gaattgggcg ttgattcttt agaatttgat ttgatggctt ctgatgatga
    13201 ttcatcgaaa aaagtaatcg aatatgttca aaactcaatc caagaaaact tggatggcgt
    13261 aacagtcaaa ccaacacctg ttccattctc tgtacgtctt gaccgctcaa cttcgggtga
    13321 ttttgacgca gtactaggcg gatgggctgc tgactatgca gacccaagca gctttactga
    13381 tttgtttgtc acaggtaatt catacaaccg tggtcgctgg agcaatgcag aatatgacga
    13441 agcagttaaa gcagcttcat caacagacgc tggcgatgaa caagcccgtt gggatgattt
    13501 ccaaaaagca atcaagatca tctctgatga tatgggtgtc gttccggtgt accaaaaagc
    13561 agaaggtcac ctagtgaatg aaaaaatcaa aggtatcgtt caccatgcgg caggcgcttc
    13621 atgggattac aaatggacat acgttgaaga ataatcgtta ataccgtgag catgagaaaa
    13681 aacgaggctg cggcaaatgt cgcaacctcg ttctcatatt cacttatcct aacaaagaaa
    13741 aaagcatgaa aatagcagaa agagcaatta gtattccggc aatgatcagc caaaagctgt
    13801 acataccgta gctggcagac gagagcgtgg aaaattctgg ttttttcttt cggttccttg
    13861 ttcgtgcaag atggaccgat cgctgaaaat gatcgaagaa acccgaagaa aagacccata
    13921 aaaaaccgcc gataatcaga aacggcaatg accataagaa tagatcatta gataattgaa
    13981 tgagtgtaaa ggaatggttg atgagatttt ttattaaaat aaccagaatc agtagaaaag
    14041 ctactgcgta ggggatagat tttttttcat ttgattgctc ctttattttt ggttcattat
    14101 ctaataatgt agcgaaacat ctgtcagaag tcaaaagcta gggggaaaca ttagtgaata
    14161 gttatatcaa atatgtatta aaacgtgtct ttttcatgat catcacgctt tggttaattg
    14221 caacaatcac ctttttcttg atgcaattgc tgccgggcac gccatatacg aatcaggaac
    14281 gtttgagtcc tgaaacaatc gagatgctaa ataaacaagt cggtttggat aaaccagtta
    14341 tcgtacaata tgggatttat ctttctaatt tgcttcaagg agattttgga atttccttcc
    14401 aatttaaaaa ccagccagtc gcacatttgt tagctggaag aatcggacca tccttgcagt
    14461 tagggttaca ggcaattatc tttggaacag tcctagggac gattttagga acgatctctg
    14521 ccatgaagca aaatacatgg gcagatacat cttctacact cgtagcgata ttaggtcgtt
    14581 ccattccaaa ctttgtattt gctgttttat tacaatatat ctttgcaatc aaattacaag
    14641 ttttgccaat cgcaaaatgg gatggctttg tgtacacaat cttaccaaca attgcgttgg
    14701 ccatgtcgcc gcttgctgac tctgctcggt ttatccgtac ggaaatggtg gaagtactgc
    14761 atagtgatta tgtggaatta gcaaaagcaa aaggattgag ccgttgggaa atcgccttta
    14821 aacatggtct tcgaaatagt ttgattccat tgatgacttt attaggaccg ttagctgtag
    14881 cattgatgac cggatcactg gtggtagaaa atatctttgc tattcctggt atcggggaac
    14941 aattcgttaa gtcaatcaca acaaatgatt atcctacgat catggctgta acgatccttt
    15001 actcatttat gctgattttt gttattttga tcgtggatct attgtacggt ctagttgatc
    15061 caagaattcg tgtttcagaa gggagccgag gataaatgga aatgatacca aagcgttatg
    15121 aaacagttgc agatatccct gcggatgaat ttcttccatt acaaaaaaat accgaagaag
    15181 aacgtgaaca gattactgca ccttctttga attttatcca agattcatgg cgccgtttaa
    15241 agaaaaacaa agctgccgtt atttcaatgg ttttactatt ggtcatcatt ttgatctctg
    15301 tgatcacaat ctttgtttct cctcatgatc caactgccca aaacgtggct tatatcaatt
    15361 tgcctcctcg tattccggga atcaatatcg atggattgaa tggaaaagca atggttggtg
    15421 gtgaacttgt tgataaatat gctcaagcaa acgtcccttc aaacgtaaat ttctttttag
    15481 gaacagacgg tttgggacgt gatgtattaa gccgcttatt tatgggaatg agaatctcgt
    15541 tgctgatcgc atttattgct gcattactag atgtaacgat tggtgtagct tacgggttgg
    15601 tttctggttt gttaggcgga cgtgtcgata acgtgatgca acgttttttg gaaatcttat
    15661 caggaattcc aaacctcgtt gtcatgattt tgatgttggt cgtttttgaa ccaggaattt
    15721 tttcaatcgt ggctgctatg gccatcacaa actggatacc aatggcacgt atcgttcgtg
    15781 cacaaacaat gaaattaaaa gatcaagaat atgttttagc tggattgaca ttaggtgaat
    15841 caagaatgaa aattgcattc aaacatattt tgccgaatat ctctagtgtc attattattc
    15901 aaatgatgtt tagtattcca tctgctattt tcttcgaagc ctttttaagc tttatcggtt
    15961 taggtcttac gcctccgtca gcttcattag gaacgatgtt gagcgaaggg tacaagacat
    16021 tcctgtatct gccttatcta ttatggattc cggcagtaac tttatcggtt atcatgattg
    16081 gctttaactt acttgctgat ggattgcgtg atgcctttga tcctaaaatg aaagagtgaa
    16141 gacgatgacg aaaattttag aagtgaaaga cttagaaatc tcttttgata cgtatgctgg
    16201 aaaggtacga gcgattcgcg gtgtcaattt tcatttgaat aaaggagaaa cattggcgat
    16261 cgttggggaa tctggaagtg gaaaatcagt gacgaccaga agcatcatgc gcttgctgtc
    16321 aagcaacgcc aatatagatg ctggtcaaat cctctttaaa ggtaacgata tcgttggaaa
    16381 atcagaaaga gagatgcaaa aaatccgtgg taaagaaata gcgatgattt tccaagatcc
    16441 aatgacctct ttagatccga cgatgcccat tgggaagcaa gtcgctgagt cccttagaaa
    16501 gcataataaa gtttcaaaaa aagagggaca aaaagctgca ttagaattgc ttaaattagt
    16561 tggaataccc gaagctgaga aacgtatcaa cagttatccg catcagttct caggaggtca
    16621 gcgtcaacgg atcgtgatcg cgattgcact ggtatgttat ccggaaatcc tgattgctga
    16681 tgaacctaca acagcgcttg atgtgactat tcaagcacag attttggagc tgctgaaaga
    16741 aattcagaca aaaatcgata catcaattat ctttatcact catgaccttg gtgtagtagc
    16801 gaatgtcgct gatcgtgtcg ctgtcatgta tggaggaaga atcgttgaag ttggaacatc
    16861 ggaagaaatt ttctacaacc cgcaacatcc ctatacttgg ggactgcttg gttcaatgcc
    16921 gacattagat agtgcgaacg atcgattgta tgctattcct ggttctccac cggatttatt
    16981 gaacccgcca aaaggagacg cattctatcc aagaaatgaa ttcgcgatga aaatcgatgc
    17041 agaacaagaa ccaccatttt ttgaactatc aaaaacacac aaggcagcaa cttggctact
    17101 tgctccgcag gcacccaaag tgacaccgcc agaagaaatc caacgtcgct gggctatctt
    17161 taaagagaaa caaaaacata cttacggtca aggaggaatt gctgatgcca aataaactac
    17221 ttgaagttaa aggattgaaa cagtatttca atgtaggaaa aaagaatgaa gtacatgctg
    17281 taaatgacat cagctttcac atttatgaag gagaaacatt cggtttagtt ggagaatctg
    17341 gaagcggaaa atccactact ggccgtacga tcattcgctt gaatgaacct actgatggtg
    17401 agatattatt cgacggtcaa gatataacga agatcaaaga taaaaaaggt ttgacgaaat
    17461 tccggcatga tgttcagatg atctttcaag atccatacgc ttcgttgaat cctcgtatga
    17521 aagtcagaga tatcattgcc gaaggaattg atgttaatgg actagctaaa tcatctgaag
    17581 agcgtgcaaa aaaagtcgat gatctattga gaactgttgg attgaatcca agccacggta
    17641 ctcgttatcc tcatgaattt tcaggtgggc aacgccagcg tattggtatt gcacgagcat
    17701 tggcagtaaa accgcgtttt atcatttgtg atgaaccaat ttctgcacta gatgtgtcga
    17761 tccaagccca agtggtcaat ttgttgcaag atctccaaaa agaacatcaa ttgacctatc
    17821 tatttattgc acatgatttg tcgatggtta aacatattag tgaccgaatc ggtgtaatgc
    17881 ataacggctt gttattagag atgggaacaa gtgatgaaat ctataatcac ggtgttcatc
    17941 cttatacaga aagtttgtta tctgctattc ctctaccaga tccagaccat gagagaaaac
    18001 gcagaagaat caaatatcaa cctgaaccag atgacggaca agtacgtcaa cttcgtgaaa
    18061 tcgcaccaga acattttgtc tatgcaactg agcaggaagt cccatattac gcaaaaaaac
    18121 taaaacgaca aaaggaatct cttctggttg ctaactagga gggacataga tgctaaaagc
    18181 ttttaaattc cgtatgtatc cgacagaaga acaaaaacag caattgattc gtacttttgg
    18241 atgtgctcga tttacctata accatttgct gaaaaagcgg cagaaaagtt ggcaacaaac
    18301 aggagtagcc aatttttcgc tcacacccgc tacattaaaa aaagaatacc cattcttaaa
    18361 agaagtagac agtcttgctt tagcaaatgc acagcttaat ttggatcggg cttttcgcaa
    18421 ttatttcaag ggacgtgcca gcttcccaaa gcttaaaacg aaaaaaagta tgtggcaatc
    18481 ttatacgacc aacaaccaaa cccgtacgat ctatctggaa aatggctact taaagttacc
    18541 aaaacaaaaa gaactaatca agataaatca gcatcggcca gttgaaggat cgattcgctc
    18601 cgctacgatt tctgctcgat ataatgaaga attttatgtc gcactacttt gtgatgtttc
    18661 gccagtaaaa aaagaatctt tagctaaatg gatcgggatc gcctatcatc cgaagacatt
    18721 gatccaaaca tctcgacctc tagaagtaac tttaccaaaa tttcaccaga cagaagaaaa
    18781 gctgcaacat gcacagcgga aattgaatgt gaaagtccgc tccgctcacc atagaaagat
    18841 caggttagat caggcaagca attatcagaa acaaaagaga aaagtaatgg atctttatct
    18901 gaaacaaaag aaccagcgag aagattatct tgaacaatta tctgggaaat tggtcaaaca
    18961 atatgactac ttatttgtag aatcttttcc aaaagaagag gcacatgctg atttttctat
    19021 tcacgactgg cataaattaa taacaaaatt gcgttataag tcccaatggt ataataaaaa
    19081 atttcttttg atcaataccg acggagcaga agaaagcaat tccgtaagaa agagccaagt
    19141 agtagaaaaa atgggcagac attcggttat caagggataa aaaatgaatg aaactgatag
    19201 gagcgttagg gttagccttg ttaataaaaa gtcttaatga cttttgtttc taggaatttt
    19261 tcttttgaag aaaagctcac taattatgac aaaccaataa aacaatagca gaaaaagttc
    19321 gtacgagctt tttctgctat tgttaatttt ttattcagaa agtttacgaa tacatcaaaa
    19381 ttctgaaaca aaaaacgtaa ctcacaaaga gttgtttttg cgttttttct tgaatgatta
    19441 ggtctgaatc ttttgctcac aaccttattc atggtgcccc ggcttgagct gatagatttt
    19501 tgaaggacgg ccgattccta caggacgttc accgatctca tcgaaatatt ggagcatagc
    19561 tttcttaaaa ttggaatgat cgatcgtttt ataatcgatc cctaagaatt tagcaaatac
    19621 ttttcgagct tccgtaatcg tgaaatcttt acctaaaact tgtagaactt gcggctcgtg
    19681 ttccatttta ttgactacac ggttgaatgc tttcaagatg atttgactat ggtcaaaagc
    19741 caaagtatct ttgccagaag actctccagt gactaaatca agcgagatag ccacttctcc
    19801 gctagataaa tggatcttgt tactatgacg ttctagtgtg aaccagcgta cttccttggc
    19861 atcatctccg gcgattagcg gttcttctcc aataaaggct aagtagctga ctgtgacgac
    19921 ccagcctcgc ggatctcgat taggcgtact gaatgtatgc agttgctcaa tattttcttt
    19981 tgaaataacc acaccagttt cttcctttgt ttctcttaag acgctttctc cagttgactc
    20041 atccttttgg acaaagccac caggcaatgc ccatgaattt cgataaggat gcccttttct
    20101 ttggatcaac aaaactttca gctgatcttc ttctttgtta tagcacatca gtacgatatc
    20161 gacagtcaaa gagggcttgt catattctgg acgttcctgt ttttgatacc agttaagaaa
    20221 ttcttcttca cttgctttat gttcataata taatttttct tcttctttag aagcaaaacg
    20281 gttcatctga ccacctccat tgacagtaaa agtacttttt accttattgt agcagactct
    20341 gaaaaaaaac caaggattta cttgaaaaat ctaggttatc atttaaatat gaaaaataaa
    20401 tggaagtatg agattgaata aaaagaaaag cagataaaac ggcaatactt tataccgctc
    20461 ttcacgaatg gcaaagaagc cacaaataac gatatatacg tcaatgataa aacgcaaaat
    20521 ccaagctgct gtcataaaac tgttcaaatg cccatggagg ttaaaataac catacatacg
    20581 aatataaaaa tagctgaata ttaaatgtgc taatgaaaga aaagcaatac taagtgtaaa
    20641 aaaaagatgt ttcttctgca tgattgttcc agtcccttac taagatagat agtctttatt
    20701 ataccacgtg tgaggggaga aataaaaagc cgttttttag tagatatgaa acaaaacaaa
    20761 aacccgcatt tattcaacct attcatcctt ttaaaggata gattgaacgt agatagaagt
    20821 cgtttatcca gtctttttca ttttgaaaaa aagctaacag catgcttttt ttatggtata
    20881 ctagcaagga aacgaattcg catcatgcga taaaggagga agagccccgt ggcagaaaag
    20941 gaaacttttt atatcactac gccaatctat tatccaagcg gcaaacttca tatcggcaac
    21001 tcgtatacta cgattgcatg tgacgcaatt gctcgttata aacggttgat gggctttgat
    21061 gtgttttatc tcactggagt ggatgaacat ggtcaaaaaa tcgaaacaaa agcagaagaa
    21121 ctaggtgtaa aaccgcaaga atatgtagat aaaatggcgg ctgatgtcaa gaaattatgg
    21181 aaaacgcttg atatcagtta tgataaattc attcgcacga cagatgatta ccataaagca
    21241 gctgttcaaa aaatatttga tcgtttatta gaacagggag atatttactt aggtgaatat
    21301 gaaggctggt actctgtttc tgacgaagaa ttctttacag aaacacaact tgcagaagtg
    21361 tacaaagatg acgaaggtaa cgtgatcggt ggtaaagcgc caagtggaca cgaagtagaa
    21421 ttggtaaaag aagaatcata cttcttccgc atgagcaaat acgcagaccg tctattggaa
    21481 tactatgaag aacatcccga attcatccag ccagaatctc gtaaaaacga aatgatcaac
    21541 aactttatca aaccaggatt agaggatctt gcagtttcac gaacaacttt tacttgggga
    21601 gtaccagtca agagtgatcc gaaacatgtg gtgtatgtat ggatcgatgc attatccaat
    21661 tatattactg cactaggata tggctccgaa gatgaaagtc tattcgaaaa atactggcct
    21721 gcagatgttc agatggtcgg taaagaaatc gttcgctttc atacgatcta ttggccaatc
    21781 atgctgatgg cccttgattt gcctctacct aaaaaagtat ttgggcatgg ctggctattg
    21841 atgaaagacg gaaaaatgtc taaatcaaaa ggaaatgtcg tttatcctga aatgcttgtg
    21901 gaacgttatg gtttagatgc actccgttat tatttgcttc gtgctgttcc gtttgggtca
    21961 gatggtgtct ttacgccaga agattttgtt tcccgtgtca attatgatct ggcaaatgat
    22021 ctagggaact tattgaatcg aacgattgct atgatcaaca aatattgtga tggactcgtt
    22081 cctgactatg cctcacttgt cactccattt gatagtgaac tgtctacgac ggctgctaac
    22141 gttgtcggac gttaccatga tgcaatggaa aaaatggaat tcaatacagc attggctgaa
    22201 atctgggtgc tgatttctcg tgcaaacaaa tacattgatg aaacagaacc atgggtatta
    22261 gcaaaagacg aagaaaagaa agctgaattg aatagtgtca tgatccattt agctgaatct
    22321 ttgcgtatcg cagcgatatt acttcagcca atcatgacag aaacgccaga gaagatcttc
    22381 aatcaactag gattggactc tgaaacaatg aatcttgaag gattacactt tggcgaattt
    22441 ccttcaggaa caaaagtagt tgcaaaaggt acaccaatct tcccacgttt agatatggaa
    22501 gaagaagttg cttttattca agaaaaaatg tctgaaggta ctcaaacaaa cgaagataca
    22561 gtcaaatggg atccagaaga aacagaactt gtttcaacga aagaaaaaca aatcaaattt
    22621 gatgtatttg aaaaagtaga actaaaagta gcagaagtca tcaactgtca aaaagtagaa
    22681 ggggcagata agttgctgca attccgacta gatgctggag acagccaaga ccgtcaaatc
    22741 ttgtcaggaa tcgctgaatt ttatcctgat ccaagcgaat tgatcgggaa aaaagtcgtc
    22801 attgtagcaa atctgaaacc acgaaagatg cgtggacaaa tcagtcaagg aatgatcctt
    22861 tctgcagaag caccagatgg ctctttacaa gtaatcgaag caccaaaatc catgccaaat
    22921 ggctcagaaa tcgcatagaa attcaaaggg ggtatgacac tagttgtgtc ataccccctt
    22981 tttccgaaat ttgagaagca attttcgtaa attccttctt attacttgaa gccaatcata
    23041 taatacagaa acagagaact atccaaaata aatcctagaa aaagaagtac gagcttcgct
    23101 gatttttgtt aatttagaat aatactaata ggaatcgtaa aaacagaaaa aaagcataat
    23161 gataaagggt cgggcagatt ttaattgatt catctggtac tcttttttat tgtccttaca
    23221 ttcataaagt ttgaatgcaa tgactttatt acatctatat ttatatctaa cagtagcgtt
    23281 gatgtgttaa ttataagttc tatcacttta tgttcaatta gtttgtgctc ttatatgtgt
    23341 gattgaaaat atagataaaa aaacaatcat cggctttgac ttcgatgatt gttttcttta
    23401 ttttgtaaaa atcctatcac ttgattttat ggtaaccttt tatttttgtc ttcaaacggc
    23461 ttggtaagag agcagtaagc agtgcaaatg ggatcgaaaa gacataccag aaaatcgtac
    23521 gtccgatacc ataatgttga tagagctttg tcaacatcac gtgtaacaag atcaacccta
    23581 aaagcaagga gccagctata aaccaatgat tatcccaatg catgacttca ctaagtgcca
    23641 tcactccaaa aggatagaaa taaaaataga tcccgactaa ggtcatccgc cagttctttg
    23701 taaatgttcc gatattcaat aaaaagaaaa tcgctgaaag caaaataccg ataggcggaa
    23761 aaagcccaac caaaaaggaa tacaacatcc cccacaagat catcacagga cctttgatca
    23821 atgcaacgat tgcgatgaac aaagtcagca atattagttt ttgtgataac gttgcaacag
    23881 tcattaatcc aaataaaatt aatgtgaacc aatgccaatg ggactgctgt tgttctttac
    23941 gggaaaaatc gacccggttg gataaataat ctgctaattt cggataagaa cgcttaaaat
    24001 ccataaactg ttcctccttc gtggaaaagg taggtttagt atacgaaaaa taaaaaagaa
    24061 atacatccgt cttaagaggg agtttttcac tgaaagtgga gaaaaaagaa tggtaaatta
    24121 aaatattctc agttatactg aaaattatac caaatttcaa agaagaaaga ggatgggaat
    24181 gaaaggcaaa ctaaaacgag aaatcaatct gtttggcgca ttagctaccg ttatgggaac
    24241 cgtgatcggt gcaggcgtct tcttcaaaac tgctgctgtt actgctagta ctcagtctac
    24301 aagtttgaca ttgctagctt ggcttttggg tggtttctta acgatctgtg ctggattgac
    24361 agtggctgaa ttagctacag caatcccgga aactggtgga gctgtaaaat atattgaagc
    24421 tgcgtatgga aaattgccca gctttttatt aggatgggcg caaagcttga tttactttcc
    24481 tgctaatatt gctgcattat cgattatctt tgcgacacaa ttgacgaact tattacaatt
    24541 atctaccgat tatcttttgt tgattgcagt gatcaccgct gtttctgtga cagggttgaa
    24601 tttgttaggg acaaaagtcg gtgcttctgt acaatcagtg acgttgattg ttaaattaat
    24661 accaattgct gtgattgtca tatggggatt attgacccca ggacaaggaa ctgtccagtt
    24721 atttccaatc gaagctggaa aagatgttac ttttgtcgaa ggattgagca gtgcgttgtt
    24781 agcaacgttg tttgcttatg acggctggtt aggtgtcggt gcaatggcag gggaaatgaa
    24841 acggccggaa aaagatttgc ctaaagcaat tattcttggt ttgagttttg taacagtcgt
    24901 ttatctattg atcaattttg tttttttgaa aactttgccg attgatcatc ttgccggtaa
    24961 tttgaatgca gcttctgaag catcagacgt catttttgga ggaatcggtg gaaagctggt
    25021 cactattggg atcttgatct cggtatacgg tgcattgaac ggttatacac taactgggat
    25081 ccgcgtgcct tatgcgatgg ctttagaaga cgagctacct ttcagtaaac agctgacaaa
    25141 gctttcaaag aagtttacgg ttccttatgt tcctgcggtt ttccagttag cagtagcttg
    25201 tattatgatg agccttggtt ctttcgactt tttaacagac atgttgattt ttgtgatgtg
    25261 gctattcagt ttactgattt ttatcggtgt ctttatttta agaaaaaaag cgccggaatt
    25321 gccacgtcca tataaagtac cactttatcc aatcgttccg attattgcga ttctcggtgc
    25381 tattttcatt ttaggtatga cgatgatgac gcaaacgaaa ttagcattga tcggtatagg
    25441 tgttaccttg ataggtatcc cagtctatta tcaaaaaaag aaaaaacgct cagaagagta
    25501 aaatatttca acaacagaca gaaaaagtac gaactaggtc ctctagatct tcgtgctttt
    25561 tctatttcaa taaaacaagt tcttctattt tgatttttgc tacttctgca agtagtgctt
    25621 atgtgctaaa gtaagaatga aaaggagaga taaacatgat atttgattct catacccatt
    25681 taaatgctga acagttcaat gaagatattc cagaaacgat tcagcgagca caagaaatgg
    25741 gggtaacgaa aatggcggtc gtgggcttcg atacgccaac gattgaaaag tctttgcaat
    25801 taagccatga ctatccaaat atatacagta ttatcggctg gcatccgaca gaggctggca
    25861 gttatacgaa agaaatcgaa aataaattac aagaacaatt gactttacca aaggttgtag
    25921 cattagggga aattggcttg gattatcatt ggatggaaga cccgaaagaa gtccaagata
    25981 aagtatttcg cagacagatt gctatcgcaa aagaaatgaa cctaccaatc agtattcata
    26041 ctagagaagc acttgaagac acgtatcaaa tcctaaaaga agaagatgtt cgagatatcg
    26101 gtggaatcat gcatagtttc agtggtgatt atgaatgggc aaaacgattt cttgacttag
    26161 ggatgcatat ttcatttagc ggtgtcgtca ctttcaaaaa agcattagac gtccaagagg
    26221 ctgcagcaaa tgtaccaatc gaacgattac ttgtggaaac agatgcccct tatctggcac
    26281 cagttccttt tagaggaaag cgaaatgaac caggctatac tcgttataca gtagaaaaga
    26341 ttgctgaact tcggaatctt ccgacagaag aagtggctgc ccaaacatgg acaaatgcgc
    26401 accgattatt tggtctaagc gaacatggat aagatgaaga tccaagagat tatagtcgtt
    26461 gaaggaaaag acgatacaag aaggctgcgg gaagtagttg atgcagatac gatagaaaca
    26521 atagggtctg caatcaatga agagatcctg atgcaaattg aacatgccca agagactcgt
    26581 ggagtgatcg tttttaccga tcctgatttt tcaggagaaa aaataagaaa aatcattatg
    26641 gaagctgttc ctcaggcaaa acatgctttt ttgccgcgca cacaagctaa accaaagaaa
    26701 aagggaagcc ttggtgtcga gcatgcaagt gatgctgcta ttttagaagc attgaaaaag
    26761 gtcgtgacgc cagacatttc ccaaacggct gtttctgaaa tcactagaca agacctttta
    26821 gactatggtt taatagctgg ggcacaagca aaagaaaaac gagaaaaact aggtgacgag
    26881 ttgcgcatcg gttacacgaa cggcaaacaa ttggaaaaac gtttgaagat gtttcgtatt
    26941 acgccgaaag aactcgccca agcaatgaaa atagtggagg aacaacatga gtgaatatcg
    27001 tgaaattgcg acaccctcta gaacaaaaga gattttattg aaacatggtt tttcatttaa
    27061 aaaaagttta ggacaaaatt ttctgactga accaaatatt ttaaggaaaa tcgtagaaac
    27121 tgcgcaaata gatgaacata cgaacgtgat cgaagttgga ccaggaatcg gtgcattgac
    27181 ggaacaatta gccaagcatg caaaacaagt cgtagcattt gagatcgatg atcgtttgat
    27241 ccctgtattg gctgatacga tgcaacccta tgacaatgtg actattgtac atcaagatat
    27301 tttaaagacc gatttgtcaa cagctgttag agaaacattt catgaggagc ttccgttaaa
    27361 agtcgtagcg aatttacctt actatattac gacaccgatc atgatgcatt ttcttgaatc
    27421 agatttggtt gtggatgaac tagtcgtcat gatgcaaaaa gaagtagctg atcgtatctc
    27481 tgctgaacca ggaacgaaag cttatggttc attatcgatt gctgttcaat attatatgga
    27541 agccagttta gcattcatcg taccaaaaac agtatttgtc cctcagccaa atgtagattc
    27601 agcgattttg aaactgacaa gaagagatat accggcagtc gaagtgacag acgaaaaagc
    27661 atttttccga ttgacaaaag cggcatttca gcaaagaaga aaaacgcttt ggaataactt
    27721 gcagcattcg tatggcaaag atgaccagac aaaagcatgg ttagcaaaaa gcttggaaac
    27781 agctggaatc gatccaaaac gtcgtgggga gactttgtca ttacaagaat tcgccgcatt
    27841 aagcaatgcg atgtcagaaa atatgcagta aaaaaagagg ttgtgacaga agttgtgaaa
    27901 aagctgtcct gcatcaagta ataagaacag ccaaacaaaa atagttcctc atgtttttcg
    27961 tttgtttggg cttattaccg cagatgcaag cttttgaaca ccgtttaaga aagaggttgt
    28021 gggcctgccg tttaactcta agtcacaagg aacaatttca aaaaacagct tctcatgttt
    28081 ttgagttagt tggtcttatt acttgatgca agcttttgac cactattatg ccgaacaagg
    28141 agatgtgaca caaattttgt cacatctcct atttttgttt cttgatcaaa aaattcttat
    28201 acttgatact cacactcatt ttgtatggat ttagaaaaga atatgtgtct tgacagttat
    28261 tagtaagtga gttatactaa gtcgacattt atgtgtcaag acatattagg agagagaaaa
    28321 aatgagaaaa aattcagttc gaaaactaac gatttctgct ttattgattg ggatgggggt
    28381 cattatccca atggtcatgc ctaaaatcat gattccacca gcttccttta ctttggcgtc
    28441 tcatgtgcca ttatttattg cgatgttttt tacaccagga gttgcagtgg ctgttgcgct
    28501 aggtacgaca tttggtttct ttttaacaac gccggttatc atcgcattgc gagcattgtc
    28561 acatcttgtg tttgcgttga ttggggcttg gtacttgcaa aagcatccga gtatcgtttt
    28621 aaaaaatgga cagtttactt ttgctaattg gcgtttccaa gggttcaatt ttgtgatcgg
    28681 tgtgattcat tctttagcag aaatgctagt agtcagcatc ttttacttta ttggtaatat
    28741 gcctgaaaca tattattctc aaggatattt ttatacagta ttcatcttga tggggattgg
    28801 tgggcttatc cacagtttag ttgattataa tattgcttat tttatagcag gaacactcag
    28861 caaatatttt gatatccccg tatttacggc agcaaaaaaa gaaaatcgga aagagtcgat
    28921 tcaagggtac gcgaagaaaa cgacatatta gacatgatct ctcaaaaaaa aaactctcat
    28981 ctagtaatgg ataataaatc ctctaagatg agagtttttt gtcctaaaaa gagctgcacc
    29041 aacatcttct ggcacagctc taacaggggg caatttatgg aagtttgatg acttgttctg
    29101 tttctttatc aaagaagtgg cctttgctga tgttgaaggc aaggtcgact atttcgcctg
    29161 gtttatggaa atctcttgca tctacttttg agatgaactc agtagaacca gtttttgtgt
    29221 aaagcatcgt ttcagctcca agtaattcgg aaacgacaac ttctgatttc actacggctt
    29281 caggtgaagc ttcaagggct acttgttcac tgtggatgtc ttctggacga ataccgaaga
    29341 taacttcttt tccttcataa cctttttcaa ctaatacttt gttttttcct tcaggaatac
    29401 gaagcttaag cccatgtcca tctgtgataa caccattttt caatgtcaca ttaaagaagt
    29461 tcatggcagg agatccaata aagccggcaa cgaacatatt gacgggtgta tcataaactt
    29521 ctttcggtgt accgatttgt tggataaatc cgtctttcat gataacgatt cggtcagcca
    29581 tagtcattgc ttctgtctga tcgtgagtaa catagatggt cgttgtttca agacgctggt
    29641 gaagtttggc aatctcagca cgcatggcta cacgtaattt agcatctaaa tttgataatg
    29701 gctcatccat taagaagact tttgcatccc gaacgattgc ccgtcccaat gccacacgtt
    29761 ggcgttgtcc accagataaa gcagctggtt ttcgtttcaa gtattctgtc agacctaaga
    29821 tctctgctgc attgtccaca cgttttttga tgtctgcttt atcatattta cgtaatttca
    29881 agccaaaagc catattatca aaaacagtca tatgaggata caatgcgtag ttttggaaaa
    29941 ccatggcaat atcacggtct ttaggtgcca cgtcattcat gacttttccg ccgatattta
    30001 gttctccttc agagatatct tccagtcccg cgatcattcg caaagtagtt gactttccac
    30061 aaccggaagg accaacaaaa acgataaatt cacggtcttt gattgataga ttaaaatcag
    30121 ttacagaata attttctgca ttgtcatatt ttttatatac gtttttcaga gccatttcta
    30181 ccataatacg ttccacactt tcttttttag ttttattaac tatttaaagt ataaatgaaa
    30241 gcgttaacta acttcaatgt catgttgaac aagaattttt tctttttttt gtcaatgtgc
    30301 tagttttaag ctttaataaa tatccataag aaataaaaac tgatacactg gagtaaataa
    30361 gtatgttaac aatatcaaaa ggagtgtctc ttatgaaaat cgctttgatc gctcatgacc
    30421 gaaaaaaacc tatgatggtg aaattagcaa cagcgtataa aacaatattg gaaaaacatg
    30481 aactttttgc taccgggacg actgggcaga ggatcacgga agcaaccggc ttgccgatcc
    30541 atcggtttaa atccggaccg ttaggcgggg atcagcaaat tggagccatg atttcggagg
    30601 atcagttaga tttggtgatt tttcttcgag acccgctggc tgcacagccc catgaaccag
    30661 atgtaacagc acttatccgt ttatctgatg tctatgagat tccgttagct acgaatatcg
    30721 gaacagctga ggtgctacta agaggactgg aagccggctt cctagatttt cgaagaatcg
    30781 tccatgaaat agacaagcat ccactttcat tttgatggat aagcgtatgg aaaaataacg
    30841 tttaatgttc gcctatatgt agctataaaa gctaaacaaa ctgataatat gcgatataat
    30901 acgaacgtat tggataatat aaacaataat attagttttt cgtatttgca gtaaatgtga
    30961 aaatatgcta tagtttctgt gggaaaagat cccaaaaata atacagagta gaaaataaag
    31021 cgttaagtgc taagaggatg ggatgttgcc tcttggacga aggacgtaat gttcgcggtt
    31081 ttattttcgc attcgctgca tgatacgatg tagcagctct taaaggagat gctaaaaatg
    31141 aagaaacacc ggttagacgc acgtatgatt gctattatgg gcttattgat tgcattgatg
    31201 gtcactttgt cccgactggt agcaatcgag accccattta ttaaaatcag tgtcacattt
    31261 attccacaag tgatcatggg cattcttttt ggtccttttt ggtcagggat aggcgcagtt
    31321 ttagctgatt tagtaggaat ggccctcttc tcgaaatctg cattctttat tggatttaca
    31381 ttgaatgcat ttattgaagg agcaatctac ggattctttt tctatcgaaa agagatcact
    31441 tggaaaaatg ccattctggc tacgcttagt gtcacgctga tcatcaactt atttttgaca
    31501 ccattatggc ttgcgttgat gtaccatgtg ccattgttca gctgggtcgt ttgggcgccc
    31561 cgcctgttaa aaacagtaat atggttaccg attcaatcaa ttgccattta ttacgtagga
    31621 cgtagtattc cttacaaaaa aattttaaga agtttagcga tccacgctaa ataaaaaaca
    31681 agcagtcttc ccatcccaga agactgcttg tttttattgt gctgtgaatt gtgcttgata
    31741 gagccgtcgg taataaccat ctttttgaag cagctcatca tgggtgccca tttcgacaat
    31801 agctccctga tccatcacta atatctggtc cgcatttttg attgtggaca ggcgatgggc
    31861 aatcacaaaa ctggtttttc cttccatcat tgctaagaag gcttgttgga tttttttctc
    31921 tgtcaatgta tcgactgagc tggtagcttc atctaagatc aacatagggg gattactgat
    31981 catcgttcga gcaatcgtca acagttggcg ttgtccatct gatatcttta acccttgacc
    32041 gccaatctta gtatctaatt tttccggcaa acgttctaca aattcataca tataagaggc
    32101 ttttagggca tcattgattt ctgcgtcact tgcttccgga ttcccaaaac gaagattttc
    32161 tcgcaatgta ctgtcaaata gccaagtatc ttgtaacacc attccaaaac ttttacgtaa
    32221 tgtatcacgt tggatagagc ggatgtcatg gtcatcgact gtaattgttc cttttgttgt
    32281 gtcataaaaa cgcatcagca aattaacaag tgtggatttt ccggcccctg tttttcctac
    32341 tatagcaatg gtttgacctg gataagcagt aaaattgaaa tcttcgatca aacgctgctt
    32401 ggggtcataa gaaaaagaaa catgatcaaa agagactttt cctttgatat ttttgggttc
    32461 caaggcatag gcatctggca aatcaggctt ttcttctggc tgatcgatca aatcaaatgc
    32521 tcgttctaga ccagaaaaag cggtttgcag ttgtgtcgtg attccagaaa gttcgataaa
    32581 tggtttagaa aactgacttg agtagatcgt aaagctggaa atcacaccaa cagtgattga
    32641 cgaaccactc ttcaatgcca ataggccgcc aactaaacca atcgataaat aagcaaggtg
    32701 atctacaaaa cgagaagacg ggtttgtcaa ggaagaagaa aattgtgccc gttgtccttt
    32761 ttcatataga tcagcattta gcagttcaaa ttgctgttga ctagcttctt cttgctgaaa
    32821 agctttcacg attttctggt taccgattcg ttcggtcaca aagcccgaga tatccccaat
    32881 aatcctttgc tgtctatcga aatttgtttg tgaagcacga gcaacgatcc aactcacaag
    32941 gaaaataatt ggtgtagaac aaagaacgac caatgtcaga agtggactta gacgcatcat
    33001 aaaaataaac gaaagaatga tgattgccac tccagaaaat aattgattga aggccgcaga
    33061 aattgcaatg gaaatattgt ccatatcatt tgtaaatcga ctgacgatat ttccgtggga
    33121 gttttgatca taataacgaa gtggcaatcg attcagatga gcaaatgcat cttttcttaa
    33181 ttttgcaacg gataagtaag ctaaccgatt gcttaacact tggatgagcc attgactgac
    33241 tacgttgatt aaaacgatac caccgaatag aataagtaaa tggaacagtc gattgaagcg
    33301 gacctgtcct tctccaatca tttgatcgat actttgacca atttgtaacg tcatcacaac
    33361 tgttgcaatc ccattgatga ttcctagtat cacagctagc caaatgtctt ttgcataggc
    33421 agtaaggtaa ggaatgaatc ggcggagcgt ctgcaaggat aattttttta ctttcataac
    33481 tattcttcct cctgtgatgc gacgatttcc tgatattctg ctgattgagc aagtaattct
    33541 tcatgggtac cctgagcagc aatcttacct tgatccatca ctaagatatg atgtgcttct
    33601 tgaatagagc ggacacgttg ggagataata atcactgtac tgccaagata agtctgtagc
    33661 gccttacgca aatccaagtc tgtttgataa tccaacgcac ttaaagagtc atctaaaatc
    33721 agtaatgctg gcttggcaat caaagctctt gcaatcgtca gtcgctgttt ttgtccgcca
    33781 gagaaatttt tcccattttc catgactttt gtatctaaac catttggaag ctgacggaca
    33841 aattcttctg cctgagcgat tttcagagct tcccaacatt ctttttctgt ggcgtctttc
    33901 ttaccccatt gaaggttttc acgaatagtc ccggtaaata gtacagaagt ttggggaaca
    33961 agagcaattt gctgacggag atgatccagc tgccaagaac gaacatccat tccgtccatt
    34021 ataactgtac cggaactgac atcatagaaa cgcggaatca attgtgtcaa agtcgttttc
    34081 ccgcttccgg ttggaccaat gattccaagg actgtatttc gcttcagctg aaatgtaata
    34141 ttttggagag aaagaccaga atcaggagta taacggaagt caacatggtc aaaagcgata
    34201 attgcttcag aagaaaaatc gggagtagta ctgacctcag cttcaagaat cgaattttct
    34261 gtgtctagca cctctttcac ccgatttcct gaagcttcgg cacgtgtgaa gatcacgacg
    34321 aggtttgaaa caacgatcag tgccaaaagc atttgattca tataattgat caaagcaaga
    34381 acttgtcctt gttgcaagct accaatattt actttgatcc cgccaactgt cagcaaacag
    34441 atgatcccga cattcatgat caaggttgta gcaggtgata atagggcaga gatattggaa
    34501 acacgtaaat aattgttcgc tagatcattt gtgcttttat cgaacttctc tgtttctttt
    34561 tttgtctgcg caaatgcgcg gatgacacga acaccgctaa gattctgact aacgtgttca
    34621 ttgagctgat ctagtttttc ttgcactttt tggtagagag gaacggtttt tttgatgata
    34681 aaaaataaaa tgattgagaa aataggcaat aatgctaaga aaaacagacc tacttcccag
    34741 tcgatcacaa aagccatgac cactgacccg atacttaaaa acggtgctct gatcaataaa
    34801 cgaatgacca ttgctaaagc tagctgaagc tgattgatgt cgtttgtcat tcgggtgatc
    34861 aaggtatctg ttccaaaatg attcaactcg ttgtgagaga aagtattgat ttttttgatt
    34921 agctgattac gtaactctgt accaaatcct tgtgaagcta ctgaagcata gtattggcaa
    34981 atgatagcac aaatcagacc aatcacagac atagcgacca tccataagcc catttcaatg
    35041 atttttcctc ggtcattcat ttttagtcca ttatcgatca aggaagccat cattaaaggc
    35101 aggacaagtt cgaagacagc ttcaagaaat ttgaagaacg ggcccaaaat gatttgctta
    35161 cgatagtctt ttgcataacg taataactga atcatatgat tcctcctatt atttatttgt
    35221 ccacaattga aaaaacaagt actaccacat tgtagcttag gaaatttaga atgaaaagag
    35281 aacaagcctt ttcataaatg aaagttttat tatgaagaag ctttagaaat tgaatagact
    35341 atttttttta aaaaaaagaa aatgagaaaa catgccagtc tcttcattca taggaacgtc
    35401 tattttactt aatttttcta taaatgaatt ctgcataaaa aatatatcaa gtaaatgttt
    35461 atagatgaaa ctctatttac ttctttttta atttgttctt gcaatctttt ttgctacgta
    35521 atttttgtaa aaaagtgggt tgagagaaat ctaattgatt ttcaaagaag gagtatgaaa
    35581 atgagaaaaa tatttattcg tttattgtgg gtactatctt agaggaataa ataatagtgg
    35641 agaaaactat ggtttggcgc agattctatc aggtttgatg gaacgaagac aacctcccga
    35701 catcaagtca aaagccactt gagattttga tctgaaaata aaaaaagaaa acaaggtagg
    35761 aacatcaatg tttctacctt gttttcgtaa tcaatgggaa aaaactaaga gtggtgtacg
    35821 taatcaataa aaaggatggt gacaacagta cctctttact ctctgaaagc atcataactc
    35881 gtttttcctc attatgccaa taaattgttg ttataccaca tataaggatt tgtaatagct
    35941 aaaaacagac aggaaagttg aaatctgtga atcttatttt gtatggaagg atttatagaa
    36001 cgcaaaaatc aagataaaga tgaataaaaa tcctgtttcg gctatatatt gcgtatctac
    36061 gccaataaaa cgagtttgtt ctgtcatagc catataaagc aaaggcaaaa atacaactac
    36121 agcgagataa gatccgatga tttctcgaaa agttttccag gaagtatgtt tcctgttttt
    36181 atatttatcc gatttcattg aatagtcata gcctccttat tctttcatat gttgaaattc
    36241 cagtataaaa actggaagga actgtacgtt ttttagaata aaaattataa gggataagta
    36301 ggctgctgtc tataataaga tatatttttc aattctttat taaagatttt attgacaaaa
    36361 tgaatccgtt ttctatgatg gaggaggggt gaataagttg ttgaagaagg aaattttatg
    36421 ttacttagag aaccagacag cctttttcga tttagaacat atgaacgagg tctttacagc
    36481 acaaaggtta gctgatcatt ttggggtaaa aagaaacaca gtcagtcatt atttgaacca
    36541 gttaaatgat gagcaactac tagtcaaaat caattctcga ccagtgattt atttccacaa
    36601 agaagctttt gaaaaacaat tttttaagtt acgcacgaat ttttattttt ctatcgatga
    36661 tctcaaagag gaacagcctt ttttcgcgca accaaaagac ttattctctt ttatgatcgg
    36721 atacgacgcc agtttgaaag aaagtatcga acaaatcaaa acagctcttt actatccaga
    36781 tggcggatta ccacttttga ttacaggcga aagtggcaca ggtaaaagtt atttagttaa
    36841 tttggtctat cagtattgtc tgcttcatga tctgctagaa gattctgcac cattcatcac
    36901 ttttaattgt gcacaatatg cagataatcc tgaacttttg actagcaatt tgtttggaca
    36961 tgttaaggga gcgtacactg gtgcagaaga aaataaaaaa ggggcttttg aagcagcaga
    37021 tggtgggatc ttgtttttag acgaggtaca tcgcttaaca ccagaaggtc aggaaaaact
    37081 gttcacctat cttgatcaag gtgtgattta tcgtatggga gaacctaatt tgtcacgccg
    37141 gatcaaaaca cgtctctttt ttgctacgac tgaagagatt actagtcatt tcttaacaac
    37201 tttcattcga agaataccta tccgaatcga actacctgca cttaatcagc gtagtcgtaa
    37261 tgaacggcta gaactgattt actctttttt tctcgaagaa caaagaaaaa tggggagccc
    37321 tttgtcagtc agtggaccag ctttgtcact tttaacaagt cagaaattat ccggaaacat
    37381 aggagagctg aaaaatatcg tcaaagtttc agcagcaaaa gcatatgctg aacagcgtga
    37441 acagcctgaa atccatgtca cgatccatca tttgcaaaaa gaactacttg cgcagcctat
    37501 tactaaaaat acagcacagc aaggaatcta tattacgcaa gaccagacct tagagcaatt
    37561 aatggagaag caacagcccg aacagcagcg aatcgttaaa agttttgagc aaatcctaat
    37621 tgatttcaag aaaaataact gtctgctata ttcttgcgaa ggaaaattaa agcaagaagt
    37681 gattcaatta tttgattttt tgctgtttga gaccagtaga caagaaaaac acgagctgct
    37741 ggtttattgg acacaatcta tccgagagac ctttcgacaa atggaaactg cctatcaaat
    37801 caaattcgat ggaaacagtg tctatgcctt gagttactat ctctatcagc gaagcactat
    37861 caaatggtta ccggaagaac atgatattat ccaactaatc aaagaattag aacaacaagt
    37921 ttctcagtct tatccatcta gttatcacta tgtccaacga attctggaat taagtaagat
    37981 caaattagat attgaagaca caagtatgga tcggatttta ctgacgatct atctaaaaaa
    38041 agcgaaatgg tcaaaagaaa aacgactgcc aaaagcgatg attgctgctc acggttatgc
    38101 aactgctagc agtatggcaa atgtagtaaa tcgtttatta caagaggatc tatttgaatc
    38161 atttgatatg ccactagatg tcacaccaca gcagattgct tcagaaatcc ttgattattg
    38221 cgaaaacagc gatgtttcaa atggattgat catattagta gacatgggat ctctgaaaga
    38281 aatccatcag ttttttaaaa aacagttatc cgtaccgctt ttgattttga ataatgtgac
    38341 gacaccttta gcaatcacag taggggaatg cctgcaaaaa aatgtatcat tagaagagat
    38401 agcagaagag actgttcgac agatccagcc agaatggcga ctgctctatc cagaggaaaa
    38461 caaacccaaa gcactcatta ctacttgctt cactggaatc ggtacggcgg tccacttaag
    38521 tgaactgcta gaaaaaagct tacctacggc ttgtcagttg aaaatcatcc catatgaata
    38581 tcagcaatta aaagataaga aaaacagtga cccattattt tccatatacg aagtcgtagg
    38641 aatgattggc accacagatc ctgatatcac caatatcgac tatctttcat tagaagacct
    38701 catctcgggg gaaaagatgt ctgtactagc tgaatggttg gacactacta tgaatgcttc
    38761 agagaaggaa atattcaatc agcgcatcat tcgtaatttt tcattggaaa aagtactgga
    38821 ttccgtgact attctagata cagagaaggt gatgggagaa atcgatcatt ttatgagaga
    38881 attagaggtg cagttgaatc gaagcatcag caatcccaaa aaattagcgt tgtatgtcca
    38941 tgtgagctgt ctcattgaac gattgatccg acaaatgcca atcgaaacct atcaaggatt
    39001 agagaaatgg aagcagtgtc aaaaagaagg gttatctgct attaaatctg cgtttagtgt
    39061 cattgaagag cattatagtg tcatgatccc caattctgag attgcctata tctatgatat
    39121 tttgagtgaa gatacagagt ttctctcgat agaagaagaa ttttgaacat aagcaagttg
    39181 ttgccactaa ttttggcatg caacttgctt ttttagtatg tgtaagtgaa aagaggagat
    39241 gaaaaagtgg atagagaaat tatcttagct tcccatggaa aatttgcttc agggatatta
    39301 gattcattag agctgatcta tgggaaaaat cattctatta ctgtattcga ttgctatact
    39361 gatgagaatt ttgatttagc ggaaacagta aggcagcttt ttatgaggta caaagataaa
    39421 gaaatcattg ttctgacgga tctttttggc ggaagtgtca acaacgaatt cttacaatat
    39481 atcaatcggg atcatgtcta tctcgttgcc ggattgaatt tgccactatt gattgagcta
    39541 gtatctcggt tggacgctga agtagagaca actgttttga tccagcaggt gctaactgat
    39601 tcaaaaaaaa tgatccagtt ttgtaacgag cggctcaatc agaaaataga agaagacgaa
    39661 ttttaggagg aagaaaaatg attgtattga caagagtcga ccatcggtta ttacacggac
    39721 aagtagcttt ttcatggaca caaacaatag gcgcagactg tattttgatt gctaatgatg
    39781 atgtacctac gaatgagata cgaaagacaa cgatcaaatt agccaagcca caaggagtca
    39841 aactagtgat caaatcaatt gatgactcga ttgccgcgtt gaaaagcggg gtgacagata
    39901 aatataagtt attcatcgta gtggaatcga tcgaggatgc atataagctg gcagaagcgt
    39961 atccagaaat caaaaccatc aacttaggag ggatcaaggc aaaacctggt actcgcagca
    40021 tcggaaaagc ggtaaatatt ttaccagaag aggaggaacg cttgaaacga ttaattgaca
    40081 aacaaataga agtagaaatc cgtcaagtac cagcagataa aaaagtggat gtaagagaag
    40141 ttttgtaaga aaggaagaac ctcaatgatt gtacaagcag tcttattagg aattgttgca
    40201 tttgtcgcac aatcagaata tgcactaggg acatcgttgc tttctcgtcc gattgtgacg
    40261 gggctgttta caggaatcgt gttaggtgat gtaaaaactg ggatcattat gggcgcaact
    40321 ttagagttag catttatcgg atcgttctca gtaggtgctt cgattccacc tgatgttgtg
    40381 accggtggga ttttagggac ggcttttgct attacagctg gagccggtac agaaaccgcg
    40441 cttttactag gtttgccgat tgctacgttg acattgatat tgaaaaatat ttacttaggg
    40501 cttttgattc caattatgaa ccataaagca gacaattatg cagaagaagg aaattataaa
    40561 ggagtcgagc atatgcactt gcttgccggt atcggtcttt ccttgatgct tggtgttgtg
    40621 gtaatggttt cttatatggt gggaagcaat acgatcggaa atctattaaa tatgattcct
    40681 gacttcgttc aaaaggggtt gtcagtagct acagggttga ttccagctct tggttttgcg
    40741 atgcttgctc gtttgctgat caacaaacaa gttgcaccat attttttctt aggttttgct
    40801 atagcttctt acttagagat tcctgtgacc ggagtagcta tttttggtgc gattttagca
    40861 gttgtggtag tcaatattat gaatgtccgt caactaccac ttcaaacaac tgcaggggag
    40921 gtagaagaag atgaagaatt ctgagatcaa aaacagcaaa ataacgaaaa aagagctaaa
    40981 tcacgtattt tggcgttctt ttcaaatgga gttttcttgg aattatgaac gtcagatgaa
    41041 tatggcttat gtttatgcaa tgattccgat tttgaaaaag ttatatcagt cgaaagaaga
    41101 aatggcagca gctttgaaac ggcatctaga gttttttaac acgaccccac atatcgttac
    41161 actgatttta ggaatcacgg ctgcaatgga ggaagaaaat aaggatgatc ctgaatttga
    41221 tgtgactgcg atcgatagta tcaaaacatc tttgatggga cctcttgcag ggttaggaga
    41281 ttctttcttt tggggaactt tgcgattgat tgccacaggt gtgggtacct ctttagccat
    41341 gcaaggaaat attttaggcc ctatcttgtt cattttgatt ttcaatattc cgcacattct
    41401 gtttcgctat ctagcaacag gttggggata tcgcttagga acgggttttt tgaaaaagat
    41461 ccaggcaaac ggtatgatgg aaagtttgac attaggcgcg tctattattg gactgatggt
    41521 cgttggagca atgactgcca cgatgatcac catcaatatt cctttaaaaa tcggttcagg
    41581 agaaaatgct gtgactgttc aaagtatttt tgatgatatc gttccaaata tcttatgtct
    41641 tggagcattt ggcatagtgt tttatctatt gaagaaagaa gtgaaaccat taacgatctt
    41701 gatcggacta gctttctttg gaatttttgg ttcgtggata gggatttttt aaaaaaggag
    41761 cagagaaaat gtttacgatg caagattata tctatgaaga aaaggaagta ctaagtacga
    41821 tcttgaaaaa gaatgatttt tcgacaagag aacatatgaa aaaaacagtg aatttactga
    41881 tcttagcaac gggttcatct tataacgctt gtttagcagc gaagccagca ttggaatctt
    41941 atggtgatct tactgtcgat attcaagaac ctttttattt tttcaattat ggaaaacttt
    42001 ctccttcaat cgatactgtg attgctgtgt cacaaagcgg gaaaagtgct tctactgttg
    42061 aagcagtaaa aatgatacaa aaacaagggt tacctgttgt tgctatcaca aatgacgtgc
    42121 aaagtccact tgctttagaa gctgcacaaa ttattgattt aggtgccggg gtagaatcag
    42181 taggcttcgt tacaaaaggc tattctgcga ctgttttaca gttactactc ttagggatag
    42241 gaattggaat aagtaaaaat aaaatcagca aaaaaataga acaagactac atgcaacagt
    42301 tgcgcaaaat aatcaaccat ttaccagcga ttattcaaaa aacggaagaa ttttttgatg
    42361 gatatcaaag tttatttcgt ttagcacagc gtttcgtcac cattggatat ggtccaaact
    42421 ggggaacagc taaagaagca gagacgaaat tgactgaaac aatccgcgtt ccgtcacaag
    42481 gatttgagtt agaagcttat atgcacggcc catacctgga ggcagatgct tcgcatttgc
    42541 tattctttat tgaaggggaa agtgtaaata aggaacgttc gcaaaagttg caaagatata
    42601 tgagtcggta cgttggagag actctcacca ttaccacaaa aaaagcaaga aacgagaaaa
    42661 cacttggatt agcaatagaa tgtgatgaat atctgtctgt attagcgctt gtcgttccgt
    42721 ttcaactatt cgcttataaa accgcagtag ctaaaggtat tgacttgaac aaaaagattt
    42781 ttgaagactt tgatacagtc ttgaaaagta aattataaga ggtggaaatc atgctgaaat
    42841 tcaatgaaga agaacaaatc aaagcaataa aaggcgcact agctttacgt ccgcaagtag
    42901 aagaaatcat cgataaactt tatgcggaaa aatttgacgc tgtctattat ctaggaatcg
    42961 gtggaacgta tgcttcctcg atgcaagctg tcacttatat gaatgggaaa agcaacttgc
    43021 ctgtctttgt acagcatgct gcagaatatt acactactgg aaacaaaaga ttaacaaaag
    43081 attctatcgt tattttgtcc tctgttaccg gtacaactca agaagtagtg aaagcagtag
    43141 aacaaatcaa gaaagtagga gcgacactga ttggatttat cgataaagcg aacagtaagt
    43201 tgtctcagct atgcgatttt gtggtcactt atccagctcc tggaacagaa caaatcaaat
    43261 tttttatggc agcagatcga ttgatgtatt tgaatggaga attcgaagct tattcagaat
    43321 actatgaaca gctcgaacgt tatttaccaa cagggttagt ggaagcagag aaaaaagcag
    43381 atgcatttgg cttatcattt gcagaaaaac atcgacgtga tagtatgcat tattttattg
    43441 gagcagggaa tcaatgggga gctgtttatt catatgccat gtgttactgg gaagaacaaa
    43501 gctggcttcc atcgaaatct atccatgctg ccgagtttct tcacgggacg ttagagatcg
    43561 tagaagaaac aaccccagtc acattatttt taggcgaaga tgaacagaga gtgttatctg
    43621 aacgtgtggc taaacttttg ccaaaaattt gcagcaatta tacattgata gacacaaaag
    43681 actatccagt agaaggtatc agtgaaaaat atcgcggacg tgttttgtct ttcttattga
    43741 tgcatgtagt gacacaacgc atcgatgctc atgtggagca gttgaactgc catccattag
    43801 agatacgccg ctactatcgt cagtttgatt attaggatag actggataaa aagaatataa
    43861 aaaataaaga gagcagcgat aactgctccc tttatttttt taaaatattg gcatcaatta
    43921 cgttataaaa cacatttttt gcattatcta cagttcaact tataatctgt ttaatgggac
    43981 atgtgggtgc caaccatttt ttgttatgca atagtactat ttagttgttg catggatccg
    44041 tagtaaaagt ccaagtacaa cgagttgcgc cagtttacat agcgttttgt atccagaatg
    44101 ttgactaaaa caaaagttcc tatgtcaacc gtcaacagag acaatctgtc cgttgcagga
    44161 cctgacagat ttagaaatgt tcaatctgtc tcttatgtat tttaaatcaa tactcactag
    44221 gagaagtttc ctaacctcct aatagtaatg ctggtagtaa cattgtcaaa actgcttcct
    44281 cttaggaaca agcaaagcat aacgagtgta aaaataaaat gtagactatt gtaacatatc
    44341 attgattatt tcaagtaaaa acgataaaag aagtaaacaa acacttaaaa acaatggaaa
    44401 ttagtcccaa ctttaagaaa tgtatactat aatggaaatt atcaattatt tatttggagg
    44461 aatatccatg aaacaaaaaa tcgcagttct tggtcctgga tcttggggca ctgctttagc
    44521 acaaacattg gcagaaaacg ggcatgatgt ccgtatttgg ggaaatgtac ctgaacagat
    44581 tgatgaaatc aataagtatc atacaaatca gcatttttta cctgatttga cgatcccaga
    44641 atcgattatc ggatataaag aattggcaga agcagtggat aacgcagatg ctattttatt
    44701 tgttgttcca acaaaagcga ttcgttcagt cgcacaggaa cttattggta aaatgaatac
    44761 taagccaatc attatacatg caagtaaagg actagaacaa gatacgcata aacgaatctc
    44821 agaagttttg gctgaagaaa ttccagaaga aaaaagacag gcaattgttg tattatctgg
    44881 tcctagccat gctgaggaag tggcagtaca tgatatcaca acaattacag ctgcaagtat
    44941 tgatcaaaaa gcagcagcct atgtacaaaa actgttcatg aatgattatt tcaggattta
    45001 tacaaataat gatgtgattg gcgtagaaac tggtgcagca ctgaaaaata tcattgcaat
    45061 tggtgccggc gctatccatg gattaggatt cggagatgat gcaaaagcag caatcatgac
    45121 tcgaggattg gcagaaatca gccgtcttgg tgtcgcgatg ggtgccaatc ctctgacatt
    45181 tatcggtttg agtggtgtgg gggacttgat cgttacttgt acaagtgttc attctcgcaa
    45241 ttggcgtgcc ggtaacttgt taggacaggg acataagctg gaagaagttc tagaaaacat
    45301 gggaatggtc gttgagggtg tggcaacgac aaaagcagca gtagagctag ctcaacagct
    45361 aggtgttgag atgccaatta ctcaaacaat ctataatgtt ttgtataatg gagaagatat
    45421 caaaaaagca gcaaaagaaa tcatgttacg tgacggaaaa atggagaatg aattttctct
    45481 tcgttaaata gatgatcaat agttaatcgt cgtttaggag gaacagcaag tgaaggtcaa
    45541 aaaagcagtc attcctgccg ctgggctagg aacacgtttt ttgccggcaa caaaagccat
    45601 ggcaaaagaa atgcttccaa tcgtggataa accaacgatc cagtttattg tggaagaagc
    45661 attggattcg ggaatcgaag acatcttgat cgtaacggga aaagaaaaaa gacccattga
    45721 agatcacttt gatgcaaata tcgaattgga aaacaatttg cgggaaaagg aaaaaacaga
    45781 attactcgct ttagttgaag aaacgactca agtcaatctt cacttcatcc gtcaatctca
    45841 tccaagagga ctgggggatg ccgtgcttca agcaaaagca tttataggaa atgaaccgtt
    45901 tgtcgtcatg ctgggggatg atttgatgaa agatgatatc ccattgactc aacaattgat
    45961 gatggattat gaagaaacac aagcttccgc tgttgccgtc atgcgtgtac cagaaaatga
    46021 aacatctaaa tacggaatta ttgatcctaa aacagatagc gaaaagggtc gttgcagagt
    46081 aaaagggttt gtagaaaaac ctgaaatggg aaaagcacca agtaatttag ccattatcgg
    46141 acgttatcta ctgactccta aaatatttga gatattagaa actcaagaac caggagcagg
    46201 aaacgagata cagctaacgg atgcacttca gactcttaac cagacagagg cggtctatgc
    46261 gagagagttt aaaggaaaaa gatacgacgt aggcgataag ttaggatata tgaagacaaa
    46321 tattgaatat ggacttcaac attcggaaat tgggacttca ttaagtgcat atattatcca
    46381 gttgagtaaa aagctaagaa cagaaagtta actggaaata agtggcctgc tgagaatgaa
    46441 aggctatttg ttattaaagc ggcagcttgt gcgtcatttg cgcaactgtc gttttagttt
    46501 tttgactcta gatcagttga gtcgtggaaa acgaaagata gaaaaagact ttcgtgaata
    46561 gattcagaaa atattatgct aatggtttaa ttttttttta agttgtataa aagaaaatga
    46621 ttacataaga gaaatgaaag ccaagccaga agattcatag tagaataaaa aggcgaattt
    46681 tttaattata aaaaaaggtg tgtgttcact atgggaaaat tagtcgctgt tgcacagtcg
    46741 ctgcttgata tactcaagtg gctgatggaa ggaagcaatt ggatatttgt gttgccacta
    46801 ttgcttatta gtctatttat catgtttcgc atggtcagaa aaaaagaaac gtttctgatt
    46861 tttatctggt atgcagcatc cgttggggtt gctaccttga tggttgaatt ggcgttaggt
    46921 agttgggaag cttgcgtatt tgctttattt tttgctgtac tgtatctaat catttatatc
    46981 ccatatatga aaagaaagtc tcggataggc agaaaaggta gacatacaac tactttttct
    47041 agtacagatt ctctcgaaca aagtagaaga acgagaagaa gacggtaata tttagtaggt
    47101 atacgcaaaa aaagataaaa taatggataa actattgaga tgagagattt atttaattgg
    47161 tggaaaaaag cttttttcac ttatataaaa gaatcttttt taaaggaatg cttgttgtat
    47221 cgggataagc cattcctttt tttatgttat ttatatatat ataaatcata ccaatgtttt
    47281 tttgtatttt cttgcttgca cttggataag agattactta caataaattc agatagaaac
    47341 agctgaataa aaaaaggctc cctgaccctt agttggcgct aagaatcagg gaatgaccag
    47401 tcacacacat acaccctgac cgatcatttt gccatagtca atgcagtgca tcgacctaat
    47461 ttattgtacc taatctaggg tgaaaattct acccctatgt agatattttc catggtctgc
    47521 acgttgtttt atttgctatg tcaaggtatt gggcgtattc gttcaatgcc ttttttgtgt
    47581 ttgaaacagc aaagaccaca tgtgttactg tctaactact gtttttttat gtacttcctt
    47641 agattaggca gctgtttcta ttcatgtcaa gtatagctag gcgggagaga taaatgagtt
    47701 tatgttattt tgactattat caccaattgg aagagaaatt aaggatagaa gagccgctga
    47761 tgcttcaagc tggcacttgg atcgcgaaac aaattaatca aggtggtaga ttgcagatat
    47821 atgctagccg cacactaagt ggtgtagcat ttgaattttg ggatcaaacc ccggatcttg
    47881 taccaagtat attgattgaa aatccagccg gaggtgtcta tgaaacgcta gaaggtgcag
    47941 gacaggcaat tattgatcag ctttctgtca aatcagaaga catttttctt cttctttcga
    48001 atgaaggaag aaacccggca atcatcgaac tagctcagtg gatcaaagaa aatggtcatc
    48061 cattaatcat tgttactgga tttgatttgt cacgaagtat caaatcgcaa catagcagtg
    48121 gtttgcgatt atttgaattt gctgatttgg ttttggacaa ccacgccaat ttacatgatg
    48181 cagtcttgac tttgccaaat ctagatccag cgatctgcgg aacagcctct cttgcaacga
    48241 tctttttgct gcagcaagtt ttgtatttta cagcaaaaca gctagtccag gacacaagtg
    48301 gaaaatagtt atccacatgt tgacaaagtt ccttaaaaaa ataatgttcc acataaaaat
    48361 ggagcgtttg aaaaaaagaa aacgattatt aatgaggatt aacaacgact actttttttg
    48421 ttttagttat tttaagatgg atcatctttg attttaagct tgttttgttt aaagtttgtt
    48481 atttttataa gttgtttcac agttatgaga aatgagttat aagaatatgt agtgtcagaa
    48541 aaataaaaag tagtggaaaa agagacaaga aagaatccat atggattctt tcttgtctct
    48601 taatacacgt tgatgaaaga gtcttcccca aaactcttta caaagtaatt atagtacatt
    48661 tgttatcatt attgcaatga agtttacctg atataacaaa attgtccaac aggatacctt
    48721 tttctaatag aaataaaata cacaaaaatt gaaggataga taatggatat ttataagtgt
    48781 ttattatttt ttttgc
//
LOCUS       DANJMI010000020        48079 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000020 DANJMI010000000
VERSION     DANJMI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.020"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            66..1490
                     /locus_tag="PWU81_001321"
     CDS             66..1490
                     /locus_tag="PWU81_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1110035.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(1671..2444)
                     /locus_tag="PWU81_001322"
     CDS             complement(1671..2444)
                     /locus_tag="PWU81_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL1110036.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2441..3346)
                     /locus_tag="PWU81_001323"
     CDS             complement(2441..3346)
                     /locus_tag="PWU81_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1110037.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(3500..3820)
                     /locus_tag="PWU81_001324"
     CDS             complement(3500..3820)
                     /locus_tag="PWU81_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDL1110038.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            4294..4980
                     /locus_tag="PWU81_001325"
     CDS             4294..4980
                     /locus_tag="PWU81_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDL1110039.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            4982..5926
                     /locus_tag="PWU81_001326"
     CDS             4982..5926
                     /locus_tag="PWU81_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDL1110040.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            6125..6769
                     /gene="pcp"
                     /locus_tag="PWU81_001327"
     CDS             6125..6769
                     /gene="pcp"
                     /locus_tag="PWU81_001327"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDL1110041.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            6848..8125
                     /locus_tag="PWU81_001328"
     CDS             6848..8125
                     /locus_tag="PWU81_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110042.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(8309..9058)
                     /locus_tag="PWU81_001329"
     CDS             complement(8309..9058)
                     /locus_tag="PWU81_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDL1110043.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            9388..9768
                     /locus_tag="PWU81_001330"
     CDS             9388..9768
                     /locus_tag="PWU81_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1110044.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            9864..10373
                     /locus_tag="PWU81_001331"
     CDS             9864..10373
                     /locus_tag="PWU81_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110045.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            10450..10629
                     /locus_tag="PWU81_001332"
     CDS             10450..10629
                     /locus_tag="PWU81_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110046.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            10654..11247
                     /locus_tag="PWU81_001333"
     CDS             10654..11247
                     /locus_tag="PWU81_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1110047.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(11294..11809)
                     /locus_tag="PWU81_001334"
     CDS             complement(11294..11809)
                     /locus_tag="PWU81_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110048.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(11835..12704)
                     /locus_tag="PWU81_001335"
     CDS             complement(11835..12704)
                     /locus_tag="PWU81_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1110049.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            12830..13510
                     /locus_tag="PWU81_001336"
     CDS             12830..13510
                     /locus_tag="PWU81_001336"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL1110050.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            13663..14643
                     /gene="pta"
                     /locus_tag="PWU81_001337"
     CDS             13663..14643
                     /gene="pta"
                     /locus_tag="PWU81_001337"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL1110051.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            14766..15239
                     /gene="tsaE"
                     /locus_tag="PWU81_001338"
     CDS             14766..15239
                     /gene="tsaE"
                     /locus_tag="PWU81_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL1110052.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            15232..15756
                     /locus_tag="PWU81_001339"
     CDS             15232..15756
                     /locus_tag="PWU81_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1110053.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(16246..16779)
                     /locus_tag="PWU81_001340"
     CDS             complement(16246..16779)
                     /locus_tag="PWU81_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDL1110054.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(17643..18395)
                     /gene="xth"
                     /locus_tag="PWU81_001341"
     CDS             complement(17643..18395)
                     /gene="xth"
                     /locus_tag="PWU81_001341"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL1110055.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            18508..19428
                     /locus_tag="PWU81_001342"
     CDS             18508..19428
                     /locus_tag="PWU81_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL1110056.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            19501..20436
                     /gene="murB"
                     /locus_tag="PWU81_001343"
     CDS             19501..20436
                     /gene="murB"
                     /locus_tag="PWU81_001343"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL1110057.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            20734..22212
                     /locus_tag="PWU81_001344"
     CDS             20734..22212
                     /locus_tag="PWU81_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1110058.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVSICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            22489..24549
                     /locus_tag="PWU81_001345"
     CDS             22489..24549
                     /locus_tag="PWU81_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HDL1110059.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            24632..25201
                     /locus_tag="PWU81_001346"
     CDS             24632..25201
                     /locus_tag="PWU81_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1110060.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(25376..26182)
                     /locus_tag="PWU81_001347"
     CDS             complement(25376..26182)
                     /locus_tag="PWU81_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110061.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            26320..27834
                     /locus_tag="PWU81_001348"
     CDS             26320..27834
                     /locus_tag="PWU81_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HDL1110062.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(28237..29220)
                     /locus_tag="PWU81_001349"
     CDS             complement(28237..29220)
                     /locus_tag="PWU81_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HDL1110063.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(29225..29872)
                     /locus_tag="PWU81_001350"
     CDS             complement(29225..29872)
                     /locus_tag="PWU81_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1110064.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(29957..30589)
                     /locus_tag="PWU81_001351"
     CDS             complement(29957..30589)
                     /locus_tag="PWU81_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL1110065.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(30605..31156)
                     /gene="coaC"
                     /locus_tag="PWU81_001352"
     CDS             complement(30605..31156)
                     /gene="coaC"
                     /locus_tag="PWU81_001352"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HDL1110066.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(31153..31890)
                     /locus_tag="PWU81_001353"
     CDS             complement(31153..31890)
                     /locus_tag="PWU81_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HDL1110067.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(31887..32876)
                     /locus_tag="PWU81_001354"
     CDS             complement(31887..32876)
                     /locus_tag="PWU81_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL1110068.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            33134..33676
                     /locus_tag="PWU81_001355"
     CDS             33134..33676
                     /locus_tag="PWU81_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1110069.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            33693..34778
                     /locus_tag="PWU81_001356"
     CDS             33693..34778
                     /locus_tag="PWU81_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1110070.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            34790..35596
                     /locus_tag="PWU81_001357"
     CDS             34790..35596
                     /locus_tag="PWU81_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1110071.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            35597..36424
                     /locus_tag="PWU81_001358"
     CDS             35597..36424
                     /locus_tag="PWU81_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1110072.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            36421..37494
                     /locus_tag="PWU81_001359"
     CDS             36421..37494
                     /locus_tag="PWU81_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1110073.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            37922..40618
                     /locus_tag="PWU81_001360"
                     /pseudo
     CDS             37922..40618
                     /locus_tag="PWU81_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            40695..41183
                     /locus_tag="PWU81_001361"
     CDS             40695..41183
                     /locus_tag="PWU81_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1110074.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            41195..41689
                     /locus_tag="PWU81_001362"
     CDS             41195..41689
                     /locus_tag="PWU81_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1110075.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            41710..42519
                     /locus_tag="PWU81_001363"
     CDS             41710..42519
                     /locus_tag="PWU81_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1110076.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            42516..43337
                     /locus_tag="PWU81_001364"
     CDS             42516..43337
                     /locus_tag="PWU81_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL1110077.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            43388..45487
                     /locus_tag="PWU81_001365"
     CDS             43388..45487
                     /locus_tag="PWU81_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HDL1110078.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            45484..46602
                     /locus_tag="PWU81_001366"
     CDS             45484..46602
                     /locus_tag="PWU81_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL1110079.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            46641..47519
                     /locus_tag="PWU81_001367"
     CDS             46641..47519
                     /locus_tag="PWU81_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL1110080.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            47710..47862
                     /locus_tag="PWU81_001368"
     CDS             47710..47862
                     /locus_tag="PWU81_001368"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL1110081.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT        15458 a         8364 c         9958 g        14299 t
ORIGIN      
        1 agtaaaaata aaaaaggttt gttatacttt ttgataaaag cggttttatt ttggaggatg
       61 cttacatgga tacagagcaa cagaaaaaag gaattggttt aattccactt gctgccttag
      121 taattggttc agcgattgga ggtggcgtat ttggtattat gacggacatc tccggatcgg
      181 ctggcggccc agctattttc agttgggtac tcgtaggaac agcaatgttg atgctctcct
      241 tgtcgatcaa taatatcaat aaaaaacgtc cagatttaaa aggcgggatc tttagttacg
      301 cagaagaagg ttttggccgt ttcgctggtt ttatcagcgg ttggggttac tggctcacat
      361 gctggttagg caacgtagct tttgcaacat tgttgatgag cgcattagga tatttcttcc
      421 cagtatttga aggtgggcaa aatctggcat cgatcgtgat gagtacagtc tttttatggg
      481 gatatgcatg gctagtgaat cggggagtcg aaaatgctag tttcgtcaat gctatcgtca
      541 cattctgtaa attagtgcca ttatttttat ttattgtagt agctatcagc acgttttcga
      601 tccaacaatt cattgataac ttttctagcg acattatatt gaatgcgaat ggacagccga
      661 tcccgttttc cagtcaagta ttgagttgca tcatggtgat gctttgggta tttgttggta
      721 ttgaaggagc ctctgtcgta ggttcacgag caaaaaaaca atcagatgtt gggaaagcaa
      781 cgattctagg gatattcggc ttgattatta tttacgtctt ggtttccatg cttccatatg
      841 ggacaatgac gatgaatcag ctgcaaacga tcaaggcgca gcctgctatg ggctatgtgt
      901 ttgaaatgat ggttggtaaa tggggagcag tcgtgatcaa cggcggtttg atcatttctt
      961 tagttggtgt ttggctctca tggacgatct taccgattga aacaatggga aacatggctg
     1021 aagacggact tttgcctaaa gcatggggaa aactcaacaa aaatggggca ccaacttatg
     1081 ctatcgtatt gacgacgtta tgtacgaatg tctttttact ttcattatta ttcactgaca
     1141 gtgcttataa ctttgcttac acacttggta cagcagctat tttcttcacg tggcttttct
     1201 taggattgta ccaaatgaaa ctttcttatc agcggaaaga atgggtccaa tttttagtag
     1261 ggcttttagc aagtgccttt caagtatggg ctatgctagt cgtagccttt aatgaagtaa
     1321 tgcttgttct tgttcttttc ttgccaggca tttattttta cctcaaagca cgtaaggagc
     1381 aaaacgagcc tgtacaacat atccacttag ataagatttt acttataggt gtgacgatgg
     1441 tcggagtgat tgcaatggtt ttatttgcca ctgggatgct tcacgtatag aagtataaaa
     1501 gaagtataaa gagaggttgt gagcctgtcg tttattcaaa aacatactag gaaaggagct
     1561 gtgacacaaa tgttgtcaca gctccttttt cagaataaac ggtgtttaag agacagccaa
     1621 tcggcaataa ggctgaacgt ctctttcaca gcctcttttt tgatgagtca tcatacattt
     1681 tttcgcttaa atacgagata tcctaatgct aagaacaaaa cgatgtaagc taaattaccg
     1741 aatagcattt gattagtaga aagatgagtc aattgcttca tgatctcttc gtttccaatt
     1801 tggttttgca gattcaacat actgatcgga ttccatttga tccactccca tttttggatg
     1861 agtgcaataa ggataccaga tatgattgac gaagcaaaat aaaagacgat tcctgctgag
     1921 atggatgcgc cggaactatt gataaagcaa gcaatcatca acactaaact taaaatcagc
     1981 cataaaccga tataactacc caaagtataa gtgaacaagc tttggatcag tgtttgacca
     2041 gtagaaacct tctccgtgaa ggaaatcgaa gggaaaagaa tcagtttcat caacacagtg
     2101 acaataatag ctaaaaggta taaataaaca gaataaataa ctaatgtgat ccatttgctt
     2161 actaatattt ctcctcgaga atactttcga taaaggagat ttttcaatgt accatattgt
     2221 gcttccatcg aaatgatcgt gcttgcagct gcaatcatga taaagacaat ccaagatgtg
     2281 gcactaaaaa gctgagcagt catttcaact ggatcaatga tcgtcgtgta ttttttggct
     2341 agaagtgctg ttcctatgag caaaacaact aatagaacag ataaaacagc agttgacttt
     2401 ttcttgatca atttaaataa ttcttgtttt acaagcaatg tcatttttct tcttccccct
     2461 ttactgaatc cagaatttcc aatagagaag attccaaatc attcgtcaga tgctggacgt
     2521 ctgtcacatg gatattttgt gcagacaata gctggatcac ggttcctaat aattcttcgt
     2581 tttcttttaa aagcttgatt ttatcaggat cttccaaagt atatccagct tctgtcaaga
     2641 tcgttcgagc agctagatca tttgttgtag agataacgat gaaatcttgc gctgtttggt
     2701 tcaattcagc cattgtacat tgtttgatga tctttccttg attgatcagg atcagctcat
     2761 cgaccatttt ttcaagctcg ctgagtaagt gactggagat caagaaagag gtgccctgag
     2821 tagccaagtc ggtaatgatt tttcgcacat ctcttgttgc ttgtggatcc aaaccgttca
     2881 tcggctcatc taaaataacg atagacggat ggttcagtaa ggcaagagca attcccaatt
     2941 tttgtttcat tcccaacgag taggatttcg cttttttgtg aatgtacttt tccatcttga
     3001 gctgattcac gatagtgtcg atagcagata catcgttggt ttcagagaat aacttcagat
     3061 gatcatagcc tgttaagaat ggatagatac ccgggtattc gatcaatgct cctacttgtt
     3121 ccaacccttt atgactagag ggagaaatct cattttcacc aatacagatt gttccatctg
     3181 agtaatggat cagtcctaaa atagctttca tgatcgttgt ttttccagcg ccattaggac
     3241 ctactaatcc aacgattttg ccaggggata agctaaaact gacatcgctg agggcttgtt
     3301 tgtgaccaaa gttcttggat aattcttgga ccgaaagact tttcaaaaat attcactcct
     3361 tttctataga tagtacaagc atacacctga tgagtggaga agtcgctagg tcattggact
     3421 tatttcttat tttttcataa aaaaattgaa tattaaataa aaaggcttat tttttctgat
     3481 cagtacttat tccaagtgtc tagtggccgc tagtaatctt gatgccaatg atactgatga
     3541 gtagcatccc cacaaagaac caagtggaaa gattcagctt atcatgaaac aagaccacac
     3601 caattatgac cgaaccaact gcgccaatcc ctgtccagat aggataagag agactcatcg
     3661 gcaaactttt gattgccttt gacaaaaagt aaaaactagc aatcatgcca atcactgtgt
     3721 aaaaagaata attgatttta gaaaaaccat cactccattt catcatcgtt gaccagaata
     3781 cttccaaaat tcctgctatg attaattcca tccatgccat acttacattc ctccttaact
     3841 ataaaaaaat acacaaaaag aggaaaatca tcccttcaaa gacctaatgg aatgatcatc
     3901 ctctttatcc tacccggtgg cagattgtat atctttttct gtagtatagc acacctatcc
     3961 agatatcggc aatctcatac tttttagtaa agtgtaccaa gcactgacgg atatatcgtt
     4021 agcggaaaag aagatgaatg ctttgcaatt tcatagaaag aagttgttat ttattaatca
     4081 tttaaaaaaa acagtaacta gaccttattt ctatatatta tcgggcaata aattaaaaaa
     4141 agctttacct tacaataagt aagataattt ttgtgataaa aaaataacga aaaatattta
     4201 aaaatagtgt ctatttcgtg agaaaacaac tatatttttt atgtgttttg ctttataatt
     4261 aaaaaaatat tgtcatttta tggaggaaaa tatatggaaa attatcttag ccttttaggc
     4321 gtcttgattg ttattctcgg gtttgcattt aaattagact cgatattgat cgtaatggtt
     4381 gctttagttg ttacagcttt gactggcggt cttggcattc aaggaatgtt agaactttta
     4441 ggtacaagtt ttgtaaacaa tcgcgggatg gcgattttta tcatcattat gttggcaaca
     4501 ggaacgcttg aaagaaatgg tctgaaagaa tcagcggctt ccttgatcaa gcgattcaag
     4561 aaagtatctg ctggtatgat tgtagatatt tatgggatct ttcgaatgat ctttgcggct
     4621 tttaacgtga gttttggtgg agttgctgga tttgttcgtc caatcatttt accgatggca
     4681 ttggggacca tcgaatcaaa agggttagaa gtccatccta agcatgaaga agaactaaaa
     4741 ggtatggctt cagctatgga aaatgtctgc tggttctttg gccaagtact attcatcgga
     4801 ggagcagggg cgttgctagt ccaatcaaca ctgaaagatc taggatatga cgtaacattg
     4861 ggacagcttg ctttagtgga agttccagtg gctatcacgg ctttagttgt ggcaagtgtc
     4921 tacttctatc ttaaagaaaa gaaattagcg aagaaatatt atccaggaaa ggggcaataa
     4981 gatggacttt tttacaaatc ctgatatttt attaggcgat aaattactgg agatccttta
     5041 tatcgtgatg ggactcgtat tgatttacac tggagtaaga aatttaacag ataaaaccaa
     5101 tcctcatcgt tatgggtcag cctttttctg gacggtctta ggggtagtta ttgcaggagg
     5161 aagatggctt ccggcaatcg tgaacggtgg cttgatcttt gcgatgacca taccagcaat
     5221 tgctaagaaa gtcagcaaag gagaaagccg gcttccatca aaagaatata tggaaaaaat
     5281 gtcagacaaa ctaggaatga aaatttttat tccagctttg agtatcggtg tctttgcgat
     5341 cttgtttgct ctatttacca atttaggtgc tttagtcggc gtgggcgtcg gtgtttttgc
     5401 agccatgcta atcttgatgt tcttttctcg agataatcga ccaagtacat ttttagatga
     5461 tgcagcagat atgctgggca cagtagggcc gttaagcatg ttgccaatgc ttttagcttc
     5521 tctaggtgcg gtatttacaa gcgctggagt tgggacagtg atttccaatg cagtaggtac
     5581 gatcgttcca gaaggaaatg tgaacatcgg catcatcatt tacgctcttg gcatggtgat
     5641 atttacggta atcatgggga atgcatttgc ggccatcacc gtgatgacgg ttgggatcgg
     5701 tgcaccattc gtatttagtc atggagcgaa tccagcgtta ataggaatgg ttgcattgac
     5761 ttgcggtttt tgtgggaccc tgctgacacc gatggctgct aacttcaata tcgtgcctgt
     5821 agcgatgctg gagatgaaag acaaatacgg agtgatcaaa aaccagttgt ttattgcgtt
     5881 atttatgcta gttttccaaa ttgcttatat gattctattt aaataataga agagaggggt
     5941 tgtgacagaa taattgtcgc aacctcttct aacattgtag tggaacacta tttttttata
     6001 agaatatatt aagtttgatc tgttatgtta tttaaactat tgtttccttt atttacgctg
     6061 ttcaaggaaa atggtatgat aaaagataac catatgttaa atcaatcgga cggaggaaca
     6121 acagatgaaa gtactagtaa caggttttga tccatttggt ggggacaaag tgaatccagc
     6181 ctatgaagca gtaaaaaaaa tgccagatga aatcagcggt gctgaaatca tcaaagtgga
     6241 agtaccgacc gtattcgaaa aaagcagtca agtagtcaaa gaagccatcc aacagcacca
     6301 accagatgtc gtgatctgtg tcggacaagc aggtgggcgg tcagcagtct cgttcgaacg
     6361 tgtcgcaatc aatctagcag aagcaagaat ccctgataat gaaggaaatc agccattcga
     6421 tacggcttta gaagaaaacg gtccagccgc ttactttacc tcattgccaa tcaaagcaat
     6481 gacaaaaaat gtacaagatc atggtctacc agcgtacatc tcttatacag caggaacatt
     6541 tgtctgcaat gatatcatgt atcgtttgct tcatttgatc gaaacacaat tcccggcaat
     6601 tcgaggtggg ttcatccacg tgccattctc acccgatcaa gtgatcgatc gtccagtggg
     6661 tacgccttct atgtctttag aagacattgc ttcttctttg acttatgcag ttgaagcagc
     6721 agtagaaaat aaagaagata tttccggaaa tgccggaaca actcactaat ttagcaagaa
     6781 acactggctg gttgctaacg ttatgttagt agccagcttt ttgtaattag aaaggaaaga
     6841 aacagccgtg gaagaatggc aatccgtctt tgaagaatgg tttccaaaag aaatcagcaa
     6901 aagctatccc atcaaaattt ctaaacaata cacaagcagt caacgttggg agatctatgc
     6961 aaaattaacg aaaaaacagc gggaactagt cgataagcac cgccgatatc taatcagctc
     7021 tcgttttatg gaagaacatt atttagctgc aacagattgg gtgttttctg acttcaagat
     7081 caatccgttt tttcgaacaa aacgaagcca gcaaaaattg tattgtgagt gtggtagaga
     7141 attaaaagtg caatacatcg ttaaatcgcc aaaaaccgga aagatcctaa aactaggcat
     7201 caaccacttt gccgatcacc tgcatgtctc tccgacagtg gcagcttcga tccatcaggg
     7261 aatgacaaaa gttgatctgg ccttagacga attattatgg cttaagcaga agaatatcga
     7321 ttttcctgaa ggactatggc aaaaatactg ttttgtcttg taccaaaatc gtcggatgaa
     7381 acagccatat ttaccggata taaaattagc tcagcggctc gcagaatttc gccaagtaga
     7441 aatgccgatt tacatagcgg attatcaagc attggaaaat gaaatcaaga aaataagtga
     7501 acacatcaat gggcagtcaa aaaagcgaca gataaaaaaa gaactatttg acgactttgc
     7561 agaagaatta gtaaaggatg tagaagaatt cttgatcaac tatcgcgcat ttttacgtaa
     7621 agattggcaa tccatcgtat atgaagaagt gccagtacat cctaatgctt attttgaaac
     7681 atttatttcc gtgttgagaa agacaaaacg tcaacgaaca ccagaagtca ctgctcagat
     7741 ggaatatttt gcgaaaaacc aacgattcat ccaaccaaaa atctatctat ttatttggaa
     7801 gcaatattgt cgctatggtt ttacagaagg gttcttcgac agcattcctc ggatagtcag
     7861 aaatggtttt ttaaaagtat tgcgtaaaga acgagaagct atacaatctg ctgataagaa
     7921 agaccgtaca gtctcaaaag aaaaatggca actagtagtg aaagatatcc aatctggaaa
     7981 tgtgcaagag acaatcgaca aatggaaagg aaaacattat cgttttactg aagctcagaa
     8041 acaagcgttg gaatattatc agaagttaga agaatcactg cgtttcaatg atgaggcaag
     8101 aaagtatctg aaggaattac tataaaaaat agaggctggg acagaagcgt ttaactccaa
     8161 gaaataagaa gaaattcacg aaaattgctt ttcaaatttt tgtgaatttc agcttatttc
     8221 cgaaggagtt gcttctgatt ccgccgttta tacgtgttta gagcgtgaga caaaagtgtt
     8281 ctttactttt gtcccacgct cagttttttt atgcgtgttg ttgtgcgttg tagtattctt
     8341 cttccatttt ttgcatacgt tcacgttctt tttggttata ccaaggtgaa acggtaaatg
     8401 caaggacatc attcagcaag taaacagagc tgttcacgaa catcgccaaa cttgcagatc
     8461 catgcgtaaa tgaaatatac caaaggacca tttgagcaag ccctgaagct aaccaccagt
     8521 aatattggtt gttgtaacgc aagaaactga tgataccagc agtcaagcta atagaaaaag
     8581 caatggcatc tacccacggc cgaggatcat ctgtataacg accaatcagg aatcctgaaa
     8641 caagatagac aaggaaagta ctgatgattg cgataaacca gctttttcca gtgaattttc
     8701 ggatttttga agccatatta tgattccatt ctttacttaa caatacgggg atatccaatg
     8761 tgatcatata agcaatctgt tcaccgatac ttaaatagtt ctttgctgaa aacccaacga
     8821 taataaaaca aatcgcagac aaaataccta aaataccgtt gactgatttt gccgcattga
     8881 tggataaaat acacaataca ccaagcgtgg taccgataaa tgtaataacg gacagccaag
     8941 tgatgggact attgacaaga gtcatgacct ggcagccaag actgaaaaag aaaagataat
     9001 aattttgttg cggccagcct tttagctgat gagctaaaaa ctgaaaatat gacttcatgt
     9061 atgaggactc cttaagacac taaatgtagt gtgttctgta tttactaaca caatctattg
     9121 tatgcttttc ttgaaaaaaa actagtcttt caattataaa taatttgtgt tatgataatt
     9181 ttctgtttgt tttcttgaaa atataatttc ttactttcat ttattatatt gggaacagga
     9241 agagtgtctt ggaggaccta ctttctgttt tttttgcctt ttttcttata aaaaggaaaa
     9301 acagctattt ttttcaatct gacacattta caatggaact ggagattaat atagatccga
     9361 gcttaaacga agaataggag tgctcatatg aaccttacaa cgatccatca tatagccatc
     9421 attgtttcag attatcagaa atcacgtgat ttttatgtta ataaattagg atttgaagta
     9481 atcagagaaa attaccgcaa agaacgagat gactataaat tagatctgaa actaggaagt
     9541 gctgaattgg agatttttgg aattgctaat gcgccacaac gaccaaacta tccagaagct
     9601 tgtggcttgc gtcatcttgc tttcaaagta gacgatattg aagaaacaat tgcagaattg
     9661 cagtccttag ggatcgaatc agaaccgatt agagaagata cctttacagg aaaaaaaatg
     9721 acgtttttct ttgatccaga cggattgcca ttagaactgc acgaataaag ataacaaaat
     9781 tgtcactttt ttttcgtttc attcgatttt tcattcgttc atttcttgat attctttaaa
     9841 aaaaagaaac aggagaatga agaatgaaaa aaatgatgtt tggtgttgct gccctcatcg
     9901 taatagggat taccggtatg acgggactgc tcaatgccgc ttttcacatg gatgatatta
     9961 gtgaccagga atatcggatc tcattgaaaa aagcagcagc aatcgtaaaa aacgaaacaa
    10021 aagaacataa catatctgct attcattttg cagttgaaaa agatttggag aatcctacga
    10081 atgcaacata tgaataccag tttttttgtg aaggacaagt catagcagtc aatccatcca
    10141 ctgggaaaac aaccgtacga aatgacacgg aaaaggccgc agaacatctt tttgatatag
    10201 aaaaaacgga agagttcaaa caaccgcaag cagcgatgaa ggaagcagtc agctactacg
    10261 ggaaaaaagc aaaagtgaaa gagtggacac ttctagtcaa agatgaacgt ccttattata
    10321 aagtccgatt aatagatgga gaacaggacc gtcaggtatg gatatctgca tagaagaagg
    10381 atattgcttc tcttttttac aatataagag taagctaaga gagtaaagat gaaaggaagt
    10441 tgagataaca tggaaaaaat gaaatgccct aattgcggaa aaaaatttgc atacgaagaa
    10501 gtcaataatg tagtcgaaca taatgataaa gaaatgccag tcgtctgccc ttattgccga
    10561 actgaagcgg caagaatcgt tactcatggt tatttcatta ctgaaaagat tgaagatttt
    10621 ttaaaataac gattttgcga tttctttttc tgcatgttag aatggagaaa aggagaggaa
    10681 actatgattc gttttgcgag aaaagaagat gctccagaaa tcgccccatt gatcttagtg
    10741 attctaaaag atatggaact gccatttttg aagcagacag gggaacaaaa gactttagag
    10801 attttagaag aagcaatcac tgatccaaat tatcgttaca gttattcacg aggtatcgta
    10861 gaagaaatag acggtaaagt tgcaggagtt gcttacggtt atacagatga agaagaatca
    10921 atcatcgatc agccactggt acctatctta gagaaatatc agctagatgc gtctacacgt
    10981 ttgttcattg ataaagaaac gtttcccaac gaatggtatt tggactctat cagtgtatca
    11041 gaagatttta gaggacaagg catcggttcg cgattattag aagcattacc taaattggcg
    11101 aagaaagcta atcgctccgt catcggtcta agtgtggatg aaaaaaatcc aaaggctaaa
    11161 aaattatacg aacgccatga cttcaaagtt gtcggtcagc ggatgatcag tggtcatctt
    11221 tatgatcata tgcaaaagaa tatctaaaaa gatgtacctg aaatgcgtat ttccgcattt
    11281 caggtacatc tttttaactt aagttaagtc ggtaagaaac aggcttatcg cgacttttgt
    11341 ctttacggta gagccagtaa tctaaaaccg cagcaataca aatacaaact ggagcatctt
    11401 catcatttga gacatccaat acatagtaat ctcctgaaaa aagtgttgct ttatccattt
    11461 tcatgatttg ctgattgaaa tgatggattt gatattgatg attatagata tcaccttgga
    11521 ctgtccagtg aagtctacga atataataaa agtcaccggg ccaattgaat attttctgca
    11581 tggttcccac cttttcgaac tctttataca gctcaaaacg cgtaccgaat gtcaggctta
    11641 tctgcttgat gtgagcaacg agatcgccat tcatggcata aagagacaat gcatcgccct
    11701 ttgttcccca gcgaccaacc atcaaaaaca gggaatgtcc tttttcgttt ttgacaattg
    11761 tccgagttgc acgtccaagc tgcttttcct gaatgaaaaa ttctgacatc tatatccctc
    11821 ttttctagat aaaactacaa gtcttctgca atcgcgcgca atatggcttt tcctacgcca
    11881 gattcaaggt ttgtgtccgt tgtatatttg gcataatctt ttacttcgat ttctgcattt
    11941 cccattgcga aactgactcc tgctacttgc agcatgctga catcattgaa gttatcgcca
    12001 atcgtcatga cttcttctaa agaaatccca cgctcgtgag cgacatgggc aacagcaatt
    12061 cctttttgag cgtttttatg gttcacttcg atattgtttt gaccagagga agtaacggct
    12121 agatcgccta aaagagaaat ctgctttcca actggaccta atacagctgg cccttctgta
    12181 tgaaaacaaa taatttttaa aacttccaga ttcttttctt ccaaaatagc acgaatactg
    12241 tccacgtatg tgatgtgcaa taactccagc ttagctgagg ccatggcaat cgccatttta
    12301 tacgtaaggt gaggtaaatg ggtagccacc atggaagcaa aactttcaat tcgtttcgct
    12361 tgattttcag aataaagacc atcgttggtt gctacttcat agtaaatacc gttagcatct
    12421 agaatatcta aaacagagat tgcttgatta tttggaatcg acacggtaaa caatgaattt
    12481 ccttctttat caaatacctg tgccccgttc aaagtgatca tcgcacaatc gattcctacc
    12541 tcatctaaag ctgcgcgagc ttcttgatag tttcgaccag tagcgaccat gaactcaaca
    12601 ccggcatcat ttgcaaaacg tatcgcctcg gcattttcag gagaaacact catatgggca
    12661 tctaataatg tgccgtccat atctgatgca attaatttga tcataatgat tatcgttcct
    12721 ttctgcttct tgttcctagt ttaccatatt aatcaagtgc tgagggtgaa gattgtccct
    12781 tgtgattgaa gaaaatgtga gttattcttt ttattaggag gtggagtttt tgaaagaaat
    12841 cattcataac agttggcaag aagttttgtc ctcagaattt tcaaaagatt attatctgca
    12901 tctaagagaa tttttaaaaa aagaatatgc gtcccaaaag atccatccag atatgtatca
    12961 tatctatgaa gcattggaat tgactcctta tgaagaagtt aaagtggtga ttttaggaca
    13021 agatccttat catggagaaa atcaagctca tggcttatct ttttctgtgc aacctggcgt
    13081 aaagatcccg ccatcgttaa gaaatatcta taaagagctt tatgatgatc ttggaatcgc
    13141 acctgttcaa catggaaatc tagtgtcatg ggctaaacag ggagtccttt tactcaacac
    13201 cgttcttacc gttcgggaag ggcaagcgta ttctcatcgc gggaaaggtt gggaaagatt
    13261 aacagacacg atcattgaaa aattaaatga acgggaaaaa ccaatcgtgt ttatcttgtg
    13321 gggaaaaccg gcacaagaaa aaatcaaaat gatcgataag agtcgccata tcatcataac
    13381 atctccacat cccagtccgt tatctgcaag tcgtggcttt tttggttcca aacccttctc
    13441 gaaaacgaat gatgcgctcc ttgcgttagg agaagaaccg attgattggc aactgcctga
    13501 aacagtgtaa taaaacagcg ctttcctgtt atagtgcagt tcgctttgta tgtgaaaaac
    13561 ttaatttttc cagcattttt gactgataca gataaaataa actgttattt ttttctggaa
    13621 tagtgtatga taatacatat aaaaacgtct ggaggtcatc ttgtggaatt gtttgatagt
    13681 ttaagattta aaattgttcg ccgtgggatc aaaatcgttt tcccggaagc tactgatgct
    13741 cgtattttag gcgcagctgc ccggttgaaa gcagaagaac ttgtggaacc cattttagta
    13801 gggaacgaag aagaaataag aaatgcagcg catgccagag gaatcaaaac atctagcttt
    13861 acgatcatca gccctgataa ttacgataag tgggatgaaa tggtggatac ttttgttgaa
    13921 cgtagaaatg gaaaggcatc caaagaacaa gcagaaaaga ttttaaaaga tgtgaattac
    13981 tttggtacga tgcttacgta tatggggatt gcagacggaa tggtctctgg agcgatccat
    14041 tcgactggcg acactgttcg cccagcactt cagatcatca aaacaaagcc tggcatcagt
    14101 cggacaagcg gagcgatgat catggtacgt ggaagagatc aagaaaaata tgtcttttcg
    14161 gattgtgcga tcaacgtgaa tccaactgcg caagaattgg ctgaaatcgc cgtagattct
    14221 gcaaaaacag cagaattgtt cgatatcgaa ccaaaagtag caatgatgag cttctcgaca
    14281 aaaggctcag cgaaatcgcc agaagtagac aaagtagttg aagcaactaa aatcgctaaa
    14341 aaactagcgc cacaatacat gatcgacggc gaattgcaat ttgatgcttc ttatgttcct
    14401 tcagttgcac aactgaaagc gccagattca gaagtagctg gtcaagcaac agtctttgta
    14461 tttccagaat tgcagtcagg aaatatcggc tataaaattg ctcagcgttt cggtaacttt
    14521 gaagcaatcg gaccaattct acaaggcttg aacaaaccgg tttctgattt gtctcgtgga
    14581 gcaaatgaag aagatgtata taaattatcg attatcacag cagcacaaac attgattgat
    14641 taacaaatac acgcgaacta gccaaaaaca gcagctgctg ttcttgacta gttcattttt
    14701 tctttaaaaa aacttcatta cgcgatatac tggcaaaaag aaagaagagc gggagaagat
    14761 gaaaaatgat cgaactatca ggattaacaa tgacagaaca attcgcagag gcaattggtc
    14821 attccgcaat gccaggtgac aatctgatat tgactggtga tctaggtgcg ggtaaaacaa
    14881 cgttgacgaa aggaattgcc caaggattag ggataacgca aatgatcaaa agtccgacat
    14941 acacgattat tagagaatat agtcaaggtc ggattccgtt gtatcatatg gatatttatc
    15001 gggtagcagc tagcggagca gatttagggt tagatgaata ttttgaaggt gatgggcttt
    15061 ccgttgttga atggggaaat ctattagaag aagcactgcc ggaggattat ttggaactga
    15121 ttttagaaaa aagtgataca gatttagaaa agcggtatgt gaaactaatc tcatacgggg
    15181 agcaaagtga aaccttcaaa cagcgaattc tagaaaaatg ggctgaaaat catgagtgaa
    15241 gaagttggga tcatcttgcg ggaagcagtg ccaggagatg caaaagacat tcttttgatg
    15301 atgggacaag ttaataagga gacagaattt ttagttttgg atgaagcaga actcctgtta
    15361 ccaccagaaa ccttagaaga agagttggat tatatatacg aaagcaataa taatttattg
    15421 ctgcttgcaa tctatgaagg gacgattatt ggaacagcat ctgtcaaggc agacagtcaa
    15481 tttcgtttgt ctcacgtggg agaagtcggt atcagtattc tgcaggaata ttggggaatg
    15541 ggactaggca ctcttatgct agaagaaatc atcagttggg cgaaggaaat gggtgtactt
    15601 tttcgattgg agcttgacgt tcaagttcgt aatgaaagag ccgtacacct ttaccgaaaa
    15661 atgggcttcc aaatcgaagc ggtcatgcct agaggggcaa gaacagatct aggtgaattt
    15721 ttagatgtat ataaaatgag ttatttgatt gaatagaaga aataggaaaa gagtgagaaa
    15781 ctgtcatcct tttgagaagg cagaaaaaca cacgaaggga ctgtgacaaa agtcgtgtcg
    15841 cagtccattt tttttgaata aacggtgttc aatcagctga ccttcggtat tagctcaaaa
    15901 aacggcaaaa tatgaggaac tattttgccg tttgtttctc taatactcag gtcaaaacgc
    15961 tgatttcaca acctcttggt aaacggtgtc caaaagcttg catctgcggt aataagtcca
    16021 accaaatgaa aaatatgaga agctattttt gtttggctgt tcttattact tgatgcagaa
    16081 cagctttttc acaacctctc caataaacgg tgttctcctg ccagctcctc ggtcaccagt
    16141 cacaatattt aataaacatg agaagctatt ttttgaaatt gctccttgtg actcggagtg
    16201 aaacggcagg ctcacgacct ctttgtgtgt ttcataagta ttcttttact ttatagtcac
    16261 aagttttttc aatggatctg tcccgaattt tttttcttga taaagcagga tttccgcaca
    16321 tgcacgacta tcttccaatg catcatggtg atgatctaat tggatgttca aattttcgca
    16381 cacagtgttc aatcgatgat ttggaaattc aggaaacagg cgacggctac ttttcactgt
    16441 acataaagaa agataattag gctgttcaag atgataataa tctaagcatc ccgctaagac
    16501 ctttgtatca aaagcagcat tatgtgcaac gactaaacga tttttttgaa aatatggttg
    16561 gatagtttgc cagacttctg ggaatttcgg cgcattgcgt acgtcttctt gatggattcc
    16621 gtgaatttgg acatttcgcc agaaaaaagg agtctcaggc tgtatcaatg tatagaattc
    16681 atcaacgatc tggctatttt ttaccatgac tagcgcaaga gaacaagcac tataaggctg
    16741 atgattggca gtttcaaaat ccatggcaac aaaattcatt tctgtaaaac ctccttggga
    16801 aattactgta acatactttt gaaaaaagca aataaattta acaaaaaaca tagaattttt
    16861 tatgtaatat ttgttgagaa aagctagttt ttgaactcat tccttgtgat ttggagttag
    16921 atggggatca tgacttctcg gtattaagtc aaatttatgc aaagttgtga ggaaccattt
    16981 tcaaaaaaat ttcttacgac tcgaaagtag gcgacttttt cacaacttct ccactaaacg
    17041 gtgttcaatc agctgaccct cggtattagc tcaaaaaata gcaaaatatg aggaactatt
    17101 ttgccatttg tttctctaat actcaggtca ggacgctgat ttcacaactt ctcaataaac
    17161 ggtgttcaaa agcttgcatc tgcggtaata agtccaacca aacgaaaaat atgaggaact
    17221 atttttgttt ggctgttctt attacttgat gcagtgcagc tttttcacaa cctcttaata
    17281 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagga
    17341 actatttttg tttggctgtt cttattactt gatgcagtgc agctttttca caacctctta
    17401 ataaacggtg ttcaaaagct tgtatctgcg gtaataagcc caaccaaacg aaaaatatga
    17461 ggaactattt ttgtttggct gttcttatta cttgatgcag gacagctttt tcacaatctc
    17521 tcgataaaca gtgttcaaaa gccagtttct cggtattatg ccaaatttat gcaaaatgtg
    17581 gaaagccatt ttcacaaaat ttttcttaat actcgaaact aaacgacttt ttcacaacct
    17641 ctttatttca aatctaattc tactggacaa tgatcactgc ccataatgtc cgtatggatc
    17701 tttgcatcag ctagtcctgg ctctaaatct ttgctcgtca aaaagtaatc gatccgccaa
    17761 ccagcattgt ttttccttga attgaatcgg tagttccacc aagagtagac accttcctgg
    17821 gttggataaa aataacggaa tgtatcgata aacccgttat ccaacagtcg agaaagagcc
    17881 gctctttctt ctggagtgaa tccagcattt ttttgattcg ttttccagtt tttcaaatca
    17941 atcttttgat gagcgacatt tagatcccca cacacgatga ctggtttttg tttttttaga
    18001 ttttccaaat aattataaaa ggcttcttcc cattctaatc ggtaatctaa acgttttaat
    18061 tcgctttgag aatttggcgt gtaacaggtt acaagaaaga aatcggaata ttccaaagta
    18121 atcagacggc cttctgtatc atggatatcg atacccattc cgtaagtggc attcaatgct
    18181 ggttttttag caaaaatcgc tgtgccggaa tagccttttc gttccgcgta attccaatat
    18241 tgataataac ctggaagatc taaatcaatt tgtcctgctt ggagtttcgt ttcctgcaga
    18301 cagaaaaaat cggcgtctaa ctcatgaaaa acttctaaaa aatttttatt tacgatggca
    18361 cgaagcccgt ttacgttcca agagataaat ttcaataaaa gcacactcct tcgaattaat
    18421 ttccttacct ttgcgatact attgtacctc attttagaaa aaaatggtgt aagatgaaag
    18481 taactaaatg atccaggagg aaagtagatg aagattggtg taatcggtgt agggaatatc
    18541 gcagaaaaag catatttacc aacttatgcg aaaaaacaag gaacacttga tttttatttt
    18601 gcaacaagaa ataaagaaac aaaagaaaga attcaaaaag agtacgggtt ccaacatcta
    18661 tatgaaacga tcgatgagct tttagaagaa aaaatcgaag catgtatgat ccacgcagca
    18721 acaaaagtac attatgaatt agccaaaaaa tgtctagaaa atggtatcca tgtttatatt
    18781 gacaaaccac tgagtgtcca tctaaatgaa gttcgtgaat tgcaagaatt agctgataag
    18841 aaccaaataa ttttgatgat cggtttcaac cggcgctttg ctcctatggt agaagaattg
    18901 aaacgtatcc cagataagcg gttgatccac ctgcaaaaaa atcgaatagc ggcaaaagaa
    18961 acgacagagt tcgtccttta tgatttgttt ttacatctta ttgatacagc tgtgtattta
    19021 ttagatgaac ccattttgag aacaaaatac cagataagag aaacaaagga atttttggaa
    19081 tatgccattt tacaattaga aacacaaaat caaacggcta ttttaacgat ggatttagca
    19141 agtggcgcta atatagaaaa atatcaagta acaagtaaaa atggtactta tgaagtaaac
    19201 gatctgaccc agcttgtcat tcaagatgct gatggaaaaa aagtgaaaga atttggcgac
    19261 tggacaaaca cgctagtcaa acgcgggttt gagccaatgg tagaggcctt cttttctcag
    19321 atccgtttag gtaaatcgga aacagaattg cttaaacaaa aagaagttgt aagaagtcat
    19381 gaattatgtg aggaagtgct taaagatcac taccgtcatc aactttgaac aagactggca
    19441 agattgtatt aagcgtgata gaataatgag gacgtttgag aaaagaaagg aaaatcctca
    19501 gtgaataaag aagaaatagt tgaaagctta gccacgatta ctatattgtt tgatgaacca
    19561 ttgatgaact atacgtttac gaaaacaggt ggaccagctg atgtccttgc attcccgaaa
    19621 aaacaagaag aagtaaagca aatcatcgat tattgccgaa tccatgatat accttggatg
    19681 gtattaggaa atgcaagcaa tctgatcgtt caagatggcg ggattcgcgg tgtagtcatc
    19741 atgctgactg aaatgaaaca gattcatgtc aaaggaacga tggtcattgc ggaagcaggt
    19801 gcctcactga ttgatacgac ttatgcagct ttggcagaat cattgactgg atttgaattt
    19861 gcttgcggca tcccgggaag tgtcggtgga gcggtttata tgaatgcagg tgcgtatggc
    19921 ggagagatca aagatgtttt tgctgaagtg gatcttttgc ttgaagatgg aacattgaaa
    19981 actttgacaa aagacgaaat ggtattttct tatcgtcaca gtaaagtcca agaattacgt
    20041 gctatcgtat tggaagctcg attcagtttg caaacagggg attatgaagc aatcaaagca
    20101 agaatggatg agttgaccga attgcgtcaa tcaaaacagc cattagaata cccttcatgc
    20161 ggaagtgtct ttaagcgccc agtaggacac tatacaggtc agctgatcca acaagcagga
    20221 ttgcaaggcc tgaaatgggg cggtgcacaa gtctctgaaa aacatgctgg attcatcgtc
    20281 aatatcgatc atgcgacagc gacagattat attgaattga ttgcgcacat ccaagaagta
    20341 atcaaagaaa aatttgatgt gtctttagaa acagaagtgc ggatcattgg agaaaaacaa
    20401 gcaatatcag aaacaagcag aagtttagcc aattaataag taatggaact taaaataagt
    20461 aaaagagcag gacggtggcc gtcctgctct tttttgtggt tgtttacgca aatggcgcaa
    20521 atcttgtaca ttttaacaca ataaaacaaa aaatatagaa tatttcctca taaatttttc
    20581 cattttttgc aatattctca aaaaaaagat aaaaaatgag aataactgat gaaaaaacaa
    20641 gcataatcca tcataaaatc agtataggtg aaaagatgaa ctttcatctg catatcaagt
    20701 aattggtatg aggcattttt tgaggtgtag gatatgaagt tggattacct tatagaagag
    20761 gaagatagag aaaaactgct gctagctaga tatttagaat ttgagaaaaa gaattatgta
    20821 gtgctgccta agttatatga aacattaggt ttgtctaaat ttaaaacaag aaattatatc
    20881 atagaattaa ataaagaatt tactgaattc aaaacaaagc cagagatcca aatttcagaa
    20941 gaagcagaga tccatgtcga acgtctgaat ttgacggata tcaaaaattt aagaagaatc
    21001 tactttcaac gatcaaaaat tgctcgactg ttaacggaga tcgtggattc aaaagtaacg
    21061 atcgaaaaat ttgcagagaa tcatttttta agcatatcaa gagcctatgt aaaaagaaga
    21121 gaattagtct cttttcttga acagcatcaa ataaagttaa agaaaaacat attagttggg
    21181 gaagaaataa acatacggaa cgtattattt ggggtatatt tcggggtctt taatggtttt
    21241 tctatgccat ttagagaaag cgtcatgaat gaagcaaaga gtatcgtgca atattttgaa
    21301 tatctgttca atttaaaact gtctgcaaca aaacgaatca aattcaattt gctaaccact
    21361 attagtttgt tgagggccta tgggaatcaa ccaatcggaa aacgtttctt ttcagatgaa
    21421 tactttaaga gctcagaagg aatgaagatc atcaaggatt tatcacatat ttcttttctt
    21481 catatcccta aagagcagat cattgatgag atttcttata tcatgttgtt tttgatcacg
    21541 gaagaagaat ttgaattaga tggcgcattt ggacttgcgg aagctcattt tcatgatatc
    21601 cacatgatta gtcaaaaaat gtcagtggaa ttgattgatc aattaccatt ttcgattgaa
    21661 atctctgaag aagaaagact atatatacga aaagaacttc gtatgggtat gatgcatgtc
    21721 aatcgaaaaa attggatatt cgactttgag atgactagct ttacgtctaa aaagcagcta
    21781 cagtttttct tcgagacgta tccaatattc agtacagcaa tccaaaaggt catacaagag
    21841 aataaacgct atttcttggg aaaagatgaa cgtttcatga ttcgcttatt ttatgattat
    21901 ctctttttat tggtcagtat ttgtccggta tcatattttg aagcaccgat tcatgtctgt
    21961 attgattttt cgcaaggatc tgcttatacc gattacatta tttctcaagt gaaaggattt
    22021 aaaaattaca atattgtgat tgagagtcga atcacttctc gtacgaatat ttatttatct
    22081 gattgtttac ttgaaaaatt taataaagaa cagataattt ggaaaaatcc accgactcca
    22141 gatgactggg aatattttgg aaataccatc atcagagaaa aaaatagaca tttaactgaa
    22201 aaaatcagct agtatttatg gatggcagct ttgtctcaca tatttattag ctgatttatg
    22261 tttttttcaa agaaaaggta actagatata actagatata actagatata ttacttaaca
    22321 aaattaaaaa gtacataagt tattattccg attttcttga aacaataaaa aatcaaaaaa
    22381 aatactctcc taatagtcca tcttcttttg attttattta tataatgaaa tcatcttatg
    22441 aaaattacta cttcatgttt agacaagagt agggaggact aagaataaat gtgttcaaaa
    22501 agacgttcaa aacaactaaa taaacacgct tgtcgtttag ataaggaacg gtcgtgttct
    22561 gaaaaacact acgttaagtt tatgatgcgc acgatgctga tcagtttatc tgtaggtgtc
    22621 tatatgcatg tacccgttca ggcaacgaca caaaccatca aggtcgtacg tacctctgtt
    22681 tctgagacca ctgattcccc tatcactatt actcatactt tcactgatgc aaatggaaaa
    22741 cagctacttc ctgcattgac ttcctataat aaagtcagtt atcctccaac gataaaccac
    22801 tactggttaa ttcctaacaa aacattatgg acatcggaaa ccggaagcag tgtgccgctt
    22861 tctatctttt ccgatatgac tgatcctaca aaaggtgatc agttaaaaca gatcactgat
    22921 attttgaatc atgctttgac agatataaag gacggtggaa gcgcacaagt ttcttatgta
    22981 tatgaaaaag attattcaca gtttgaaaga aagaaaatcg atgttctggc agggacaaaa
    23041 gtttcgatat ctgatctggt agaaaggctg aagaacgcag atggaacaca aggagatatg
    23101 tccaaagtaa tttcaaacaa tgggaatatc gtggatacga ctaatacagg aacatctact
    23161 atttccttgt cctattttga tccaattgct ttaaaaacaa tgactgcatc tgctgtgatc
    23221 catgttcgtg ctgatcaaac agctatcaaa ggaaaagatc ttcacttaag aacaggagag
    23281 agttacgccc ctaaagatct tgttgaatat gtaacaaaaa gcgatggaac ttcaggaaat
    23341 aaagaagaaa tcaaaatgaa cgggacagta gatactacaa ctccgggaga atatcccgtt
    23401 gtattaactt atatagactc gaaaacatca aatgtggcag aaacaacagc tgtagtttat
    23461 gtagaagcat ctgctgcttc atcgactgtc atcactcatt ttatagatag tcaaacaaat
    23521 aaagaacttc aagcagaaac acatatgagt agtgtaaatc gtattcctga tttgcgccta
    23581 cctgctgaat tttctgatcg agtgccaaat atagatttat cgacttatga acaaaatggg
    23641 aaagtcactt ctatttctga ttggatgcaa gcaaacggat tagacagaaa aagtcactgg
    23701 tcggagatcc ttgaagtact tcactcagaa ctgatgaata ataggaagca atcaggaggg
    23761 gaaattacct ttacttatgt ttatatacca gacaatcgtt cattagaagg tcatgatctt
    23821 attgttcatc caaacgaaaa aataaacaaa gaagagttga tccaatcagc aacaaattcc
    23881 ttaggtaaac ccgtaccgtt aaaggatgta tcggttacaa tcaatgaagt accattggag
    23941 gatggttatt tgaccaaaca aacaggtgat tttacgatta tgtatagtta tcatgacccg
    24001 tattcactcc aagaactcca tgctcagtca ctattacatg ttttaccatt aaacaaacag
    24061 atagatcaaa aagaacaaaa aagacaagaa gaaagcttgt cagaaaaaaa acaacaaatg
    24121 gctcatgcag tttctccaac agggaaagta aaacaaacaa gaagcctaga gttagcaaga
    24181 atatctagaa aaatctcaga atctaataaa acgataaggc aagaagctca tttggcccca
    24241 caagtttctg gattggaaaa tgaaatttta gagcctaaaa taaaacaaca atcagaaaaa
    24301 gaatttgttg ctaaacagtc ggaagatcag aaggaatcag atgaccagaa ggaaacgaag
    24361 aaaaaacgct tgtcaactat tgattccacc gatgagcatc tgcagccgca agtaccactt
    24421 cctagtccgg gaacacccgc acctggtgga ggtattcctt cttctgcttt aggagatttc
    24481 atgcgaggta tggcaggaac atgggtatac gctaatcgaa atgaatttga cgcagatgac
    24541 tggttctgat ttatatctct atctgttata gtcaaataag tagctgagtc atatgttatt
    24601 tggcataatg gagacgagga gggatagaaa aatgttagat aaaacgttac cttacgcaga
    24661 aatctggatg acaagaccac ttcaacaaaa tgttccaatc acgcctttag cggaaggttt
    24721 tgaatttcga ttttatcaaa aagaagatga atctgcttgg gcggagattg agacttccgt
    24781 tggggaattt caaacgacag atgaagcact tgagtatttt caacaaaact ttgctcctta
    24841 tcctgaagaa cttgaaagaa ggatgttttt tgtagagaca aaaaaaggag aaaaagtagc
    24901 aacctgtacc gcttggcgaa aaaaatcaag agagcaagtg tatccggttt ttcattggtt
    24961 agcagtgaaa tcagattacc aaggaaaagg gctcgcaaaa gcattgactg ccagagtctt
    25021 gcaagaattt cctaccctcc atacggacgg accaatctat cttcacacac agacatggag
    25081 ccatcaggcg attgctcttt ataaaaaaat gggttttact tttattgctg aaaatctaga
    25141 cggcacacct aatccagatt atgaaaaagt tatgcaggtt ttaagtaaat tagataaata
    25201 acgaaatggt gaaataagtc ctcagtattg ggataaaaaa cgaaatagtc attcctcata
    25261 tcttactttt ttgagttaat actatcggat aactaatggc atgccataca ttcaaaaaag
    25321 aaggggcagt gacaaaagta ttgtcactgc cccttctctt gttttttagc atgttttatc
    25381 gtttgatttt tttaggaata aaattcttga tgaacgtaaa tttttggtaa tcgtttcgac
    25441 tatagaggta accaaataca ctgaaaacaa ctgctccaat aaaattaaca aataaatcct
    25501 tcatagtatc catgattccc acgtctaagt aaccattttg gatgattgtt ttatctgttt
    25561 taccatctgc attttgactt tcaatgattg tccgagtgat atctggaaca tgatagacag
    25621 tgtttccgtc tttacttaac tccacactat tgatttgctg gatgatcgta tctttttgca
    25681 tatcgaattg cagtaattta tctgctgaaa attccacaaa ctcccacata acaccaactg
    25741 tcatcgaaaa acagaaggaa acaactgcta gtaataaagg agtcaagttc atggtttttg
    25801 tatttttatt gagcaaataa acaagggaaa agccgacacc cccagctaaa aatccgctga
    25861 atgcatgtag aaaagtatcc cacattgaaa aatagccata aaaatcactt aactctccta
    25921 aaactgttga actaaaaata aaagataaca aaatgatttc tagcatattt ggaatctgta
    25981 tctttaatat cttttccaga aaataaggga tttcaaataa aattaatgtc agtaataaaa
    26041 gcattgtatt tccccagtgt tggttccaga cctgatccac tagcataata acgacgatga
    26101 tacgtaataa aatatagatt ccgtttgtga ttctacctgc agtttttgta ctgttcgtaa
    26161 aaaaggtttt caaatgattc ataaaataac tccttcatgt atatccttaa ttatactaag
    26221 aagcgtgtgt ttaccaaagc tatatgactt aaagaaaaac agagttgata ttgtgaaaaa
    26281 aatcacttaa tatagtataa ggagaaaagg gggatacaaa tgaaaaaatt agttgctggt
    26341 ttattaacag ccgttgcact tcttgctatg agcgcatgcg gcgcagatca gaaaaataca
    26401 gcgcagacaa gttctagtac gaaggaatct gccgaagtat catcaggcgc ttctgaacaa
    26461 gtatatacag atccaaaaga aatgaaagaa tcttatgatg tcgtgattat tggagctggt
    26521 ggtgcaggga tggcagcagc tatcgaagca aaagatgctg ggttgaatcc agtaattttg
    26581 gaaaagatgc ctgttgccgg aggaaacaca tctaaatcat caagcggtat gaatgcttca
    26641 gaaacaaaat ttcaaaaaga agaaggaatc aaagactcga atgatgcctt ctatgaagaa
    26701 acgctaaaag gtggtggagg aacgaatgat aaagaaatgc ttcgatactt tgtcgatcac
    26761 tctgctgatg cggtcgattg gctagatcaa aacggtatca aactggacaa cttaacgatc
    26821 actggaggga tgagtgaaaa gaggacacat cgtccgtctg atggctcggc tattggtgga
    26881 tacttagtgg aggggttatt gaataacgtc catgagcggg aaatccctat ttttgtcaac
    26941 gcagatgtga cagacattaa gaaaaatggg gaaacggtca atgaagtgac tgtacatgtt
    27001 caaggagaag aaccaaaaga ggtcaccgga aaagcaatcg tagttaccac aggcggtttt
    27061 ggtgccagca aagaatttat tgaaaaatat cgtcccgatt tagctgatta tattacgaca
    27121 aatcaagaag gatcaactgg agatggtatc accatgattg aaaaattagg tggacaaacc
    27181 gttgatatgg ataaaataca aattcatcca actgtgcagc aggatgaggg ggtcttgatt
    27241 ggtgaggttg ttcgcggaga aggagcaatc ttggtcgatc aagaggggaa aagatttgtc
    27301 aatgaaatgg atacaaggga taaagtatct gcagcaatta cagcattgcc tgaaaaatca
    27361 gcttatttga tttttgacca aggcgtgcgt gatcgtgcca cagctattga attttatgac
    27421 caaaaaggat atgtcacaac tggaaagaca atggaagaat tagcagaaaa aatcgattta
    27481 ccaaaagaaa cactagcaaa aacagttgat ggatggaata cggatgttgc ggagaaaaaa
    27541 gatgctcaat tcaaccgtgc cacagccatg gaacatgact tatcgtctcc gaattattat
    27601 gcgatcaaaa tcgcaccagg aatccattac acgatgggcg gtgtgaaaat caatacgaac
    27661 actgaagtat tagataaaga aaaccagcca attagggggt tatatgctgc tggtgaagtc
    27721 acaggtggct tacatggaaa caaccggatc ggtggaaatt cagttgcgga gatcattatt
    27781 tttgggcgac aagcgggcat acaagcagct cgatttgctt cggaaaatga atgaaataga
    27841 acgtaaataa aaagaggttg tgaaatcagc gttttgagct aataccgagg gtcagctgat
    27901 tgaacactgt ttagtgaaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag
    27961 ccaagtaaaa acagcttctt atgtttttaa cttggttgga cttattaccg cagatgcaag
    28021 cttttgaaca ccgtttattc ggaaaaagag cgtgggacaa aagtaaagaa cacttttgtc
    28081 tcacgctcta aacacgtata aacggcggaa gcagaagaaa ttccttcgga aataagctga
    28141 aattcacaaa aatttgaaaa gcaattttcg tgaatttctt cttatttctt ggagttaaac
    28201 gcttctgtcc cagcctcttt tttgatcagc atgattttat tcagttatct tcaaaatgat
    28261 ttttccgatg tttttgtttg cttccatata ttcatgcgct tgtttgactg cgctgaattg
    28321 gaatgtacgg tcaatgattg gccgaatctt gccattttct agaaaaggcc cagctttttg
    28381 cataaaagtt tgtgcgagct ctgctttata ctgatcactt ctaggtgtca ataatgtacc
    28441 aagcagttgg acacgtttcc caatcaatac accaaggtct atctcaggca cgatggtccc
    28501 acctagcata ccaatcaaga cccaacgtcc atcaacggct atagaagcta aattcttttc
    28561 ccagtaagaa gcgccgatga aatccaaaat aacatcgact ccccgacctt cagtgatttt
    28621 tcgtacctct tcttgaaagt cttgtttttt atagttgatc aattcatctg caccaagttc
    28681 tttacaaaag gcgagttttt cttctgatcc tgcagtaacg atgattttcg cgtgagaaag
    28741 ctgtctagct aattggatcg ctgctgttcc cacaccactt gctcctgcat ggatcaagac
    28801 agtttcgcct tcttgtaatt ttccaagcca gtaaagtgtt tgataagcag tcaaaaaaac
    28861 ttctgagatt ccagcagcag aaacatagtc tagcgtatca gggagaagga ttgcacgatc
    28921 ggcaggcatc acagcatatt cggcataacc acctagattg actaatccgt atacttttgt
    28981 cccaggagta aaagtggaga tatcactttt attctcaact acctctccag ctacttcgac
    29041 tcccaaaata gggtagggag gtttcaaaga gactgattct cttttcataa tatctgttcg
    29101 attgacagct gctgtatgga ctttgatcag taattcgcct tgttttggtt taggaacagg
    29161 cacttcaatt tgttctaata catctacaga accaggatgc ttgattgaaa tggcacgcat
    29221 ggatctattc ctcctttgcc cattcttgga atgctgtatt caactctttg ctaccttcag
    29281 aaagtactag agtcgtacga gaaatatccg tttcttccac tagagcggca agtacagcag
    29341 caactgtaga gcgagtgatc gtttttgatg ccttctcatt aggcttagtt aattgaatgg
    29401 aagtaacttc ttcatcgtta gtcaatgtga ctggttgaag aatgacaaaa tctaagttag
    29461 ttcgtttcag ccattcatct gcataatgtt tagtaatata ataatctgtc atggactcag
    29521 gccattttgc tggttcatct gcataaacag cactgaccat cagatagcga gagacatgcg
    29581 cattttcagc tgcgatcact gttttgattg cgccatccaa gtctacttgc agaagattct
    29641 ttccttgaga gccggcagca aaaatcaata catcgatgcc tttgaatgct tcggtcattt
    29701 tttccacgga ccaagttaaa tcaaaagaga catacgaaac attctctttt tttactaatg
    29761 tttgggaagc aacatctctc acccctgcaa aaatttcatg ggaacttgga gctaaatctt
    29821 ttatcaggtg tcggccaatt tgtccgtttg cacctacaac aaaaattttc atttggatca
    29881 tcctttcgac gtcaaatctt tccattcaat agtaacacca aaagagaaaa aaatcagaga
    29941 tcggctacga tgactatcag ttagtaagag aagggtgctt gaagtatctt aacaagacat
    30001 tgcatactgc tgcagtcgca agacctgcga cgattccttc aggtatacca cttgtaaaaa
    30061 taaccgctag aatagctgaa taaacagcat ttaaatttgt tccgatgact ttggcataat
    30121 cttgctggaa taaaaagtag atcatattca tcactaaaat cgtattggtc aatgaaccgg
    30181 aaagtccagc taagaataaa gaaacggaat ctgctttttg tttcattagt ttgcgcagac
    30241 ctttataaac gaaataagga accaccccaa tcaagatgcg aggaataaac gcgatcagca
    30301 aggctttcca gcttccatgt tctgttccga tgactgggat aaacggagaa aagacaaaag
    30361 acaaaggtgt ttggataact gtgctacgga tcatgctgat cagtccaaat gtaccgccaa
    30421 gaaaagcacc tagtcttggt cctaaaacga tcgaagcaat gattacagga atatgcatgg
    30481 ttgttgcatt gattggaccg atagggataa aacctaaagg cgtaacagct aataaaatca
    30541 aaatagctaa aaacatcgca gttaatgtaa agttttttgt atttttcata agtactcctt
    30601 ttttctaagc atttctttca ttaagagtca gcatgacctg ttcgatgatc gtctgaatat
    30661 cagctaaggc accttttcca aaatctccac aagctaaaag agattctctt ggttcaattt
    30721 cctgatagcc gacagatctc aatgtttcca gattacgctg agtgatcggg ttttgataca
    30781 tattcgtgtt catggcaggg gcaatcaatt ttggtgttcc cactggaagc gccattgcga
    30841 ctgttgaaag aagatcgtct gcaatgccgt ttgctagctt gccgataatg tttgctgtag
    30901 caggcgcgat gagaaacaag tcagcttgtt ttgctagttc gatatgattg attacctgcg
    30961 gatttgattc ttgcatgacg tctgtatgga ctgcgcgctt cgacagagat tgcaaggtta
    31021 atggcgtgat aaaatgtgtg ctgcttttag acataatcac atctacctgg gcacctaatt
    31081 tgacgaattg acttgtaaga tcagcgcttt tgtaagcaga aatacttcca gtaacgccta
    31141 gtaagatctt tttcattttg attcctccag tctaattttc tcagtaatca atgcagcaat
    31201 ctcagattta ctttgagctt cttccactgt tccatcttta gataagagat agccgtggtg
    31261 tgtctcgcca tggatttccg tcaaatcatt tgcgaaaata aaatcagttt tgttatttat
    31321 cagactattt tttgcgactg ccagaagaga ttcctttggt acatctacta atagtttgaa
    31381 accaatgatt tttgcttttg gctgccattc acgtagataa gaaatgattt ttggtgtttt
    31441 cttcaaaacg agcacaagat gctcggtgtt agaagagatc tttgaaacag tggtatctgt
    31501 ctgttcattt tccgcaaacc aattgtccag ttcttcttgc gttgaagaag tgggtaggtt
    31561 ttttgccagc tgttcttctg aaaaactaaa agcaggagta aaatcactga ctgccatact
    31621 gtggataatc gcatcgtagt gttctttttt cgttagttct tctaattgta aaaataagtc
    31681 cagtgcagat tcgatcgtat aaatcgttat attggagtgt cttttcggtt tcagtgcttg
    31741 tttcgtagtc acgtaatcga ttgtggtatc cggattggca gctaaataat cagctaatgc
    31801 ttgccctaat cgtcctgtcg aatgatttgt aatagcacgt acttgatcga ttttttcagt
    31861 cgttcctcca gctgtgatta gaatcttcat ttatggtcac ctacatttac tagtgttctt
    31921 ccttggtgtg ttccagcaag tagtttagat gctgtttcag gcaattgatc cagagtaatc
    31981 tcttggacga ccagttcatc tgcaatctgc aaatccgcca tctgttgcca taaagaaaga
    32041 cgttgatcta ttggtacatt gacagaatca attccaatca actggatgtt tcgcagaata
    32101 aaaggcaaga cagtcgcgtt tagtttgatt cccccagcat tgccacataa agctactgca
    32161 ccgttataag agatcaatgg aagaagactg cttagctgct cgccacctac tgtgtcgatg
    32221 acatagtcga tttgctgttt tcctaaagct tttgttgtct ctggcagaaa ttcatcaggt
    32281 cgtacgactt gcaaagcacc tagacttttt agccatgtga cagactcttc ttttctggaa
    32341 aaggcaatga ttgactggaa tcctagttta tgtaacaggg ctatcgctgt actgcctaca
    32401 ccgccagaag caccagtaac gacaactttt ttatcggagg tcataccttg acggattaaa
    32461 gcgttcacac acaacatagc agtaaaccct gctgtaccaa ggatcatcgc ttgacgcagc
    32521 gtcatatttt ttggtagagg aactaaccag tctcctggta ctttttgata ttggctataa
    32581 ccgcctggat gggtgacgcc aagcccaaaa ccagtagcaa gtacaagatc ttcttttgaa
    32641 aaacgtggat tgttactttg gacgacagtt ccggataaat caatccctgg agtcatcgga
    32701 tatgttcgta tcacctgacc agactcgctc attgccaatg catctttata attgacatca
    32761 gaataggcaa tttttaccaa gacttcattt tcagccaact cagtaaatgg cagatcactg
    32821 attgctgtat ggaaaacagg ttcctttatt acctgaaaag atcgaaaagc gttcattcgt
    32881 gagacactcc ttttaaaatt ttcttttatc ataccataaa gcctactctt ttattcttgt
    32941 tcttccgtaa atttagacaa aaattaggtg aaaaataaag aatcaaacaa ctagaattta
    33001 ttccaaaaga taaagaaata ttcagcaaaa aaaattttac aaagggttgc ctttttcaat
    33061 aaatttcgta tactaatacg agtggtagta aacaggaagt ttactattag aaaacttcga
    33121 ttcggaggaa attatggaaa ttggtgagaa gttaagaaat ttgcgcatcc aaaagaattt
    33181 gacacaagaa gaactaggcg aacgcacaga tttgtcaaaa gggtatattt cccagttgga
    33241 acgagattta agttcaccat caatggaaac attcttttct attcttgaag tcttaggtgt
    33301 gactccagaa gaattttttc atcaagaagc agcgaatctg caagtggttt attcaaaaga
    33361 agatcatacg gtttatttcg atgaagaaaa cgggtatgaa ttggaatggc tcgtggctga
    33421 ttcgaatgaa aaagaaatgg agcctgtctt gctgactttt gaaggtgcag gagaatataa
    33481 gacgtttgaa ccttcattat ctgaaacgtt tatttttgta ttagaagggg aattagagtt
    33541 aagcttagga gagacggtct atgtggcaaa agcaggacag tcgatctatt atcaagcgac
    33601 aaaacaacat caattaagga atcatctcaa aaaacgaaca aaagtattga ttgttgcaac
    33661 gcaatcatat ttgtaatata gggggtaaaa acgtggggaa accaatcatt tcatttgatc
    33721 aagtagtgaa acgctatgat gatgaaacag tattaaaaaa agtaagtttt gagatcgaac
    33781 aaggaaaatt ttatacactg cttggtcctt ccggctgtgg taaaacaacg atcctacgta
    33841 ttatagctgg attcaccgaa gctacagaag gggacatcta ttttgaaggg aaacggttga
    33901 atgatttgcc agctaataag cgacaagtaa atacagtctt ccaagattat gcactttttc
    33961 ctcatatgaa tgtttttgat aacgttgctt ttggattgaa gatcaagaaa atgtccaaat
    34021 cagatatcga aaagaaggta aaagatgctt tgcgtatggt ccagcttcct ggatacgaaa
    34081 aacgggagat tagtgaaatg tctggtggtc aaagacagcg tgtagcgatt gcacgagcaa
    34141 ttgtaaatga accgaaagta ttgctattag atgaaccgtt atcggcgctt gatttgaaac
    34201 tacgtacaga tatgcagtat gaactgcggg atctgcaaca acgattagga atcacgttta
    34261 tttttgttac tcatgaccaa gaagaagcgt tggcaatgag tgatgagatt tttgtcatga
    34321 ataaagggaa aatcgtccaa agtggtacac ccgtagatat ttatgatgag ccaatcaatc
    34381 atttcgttgc tgattttgtt ggggagagca atatcgtgga cggtgtaatg atcgaagata
    34441 atctagttga gtttgttggt aagcaatttg aatgtgtcga tggcgggatg cgtctaaatg
    34501 aaccagttga agttgtttta cgcccagagg atttgacgat cacgacacca gataaaggaa
    34561 aactagtcgt aacggtcgat actcagctgt ttcgtggtgt ccattatgaa atcatctgtt
    34621 atgatgaaca gcaaaatgaa tggatggtcc attcaacgaa aaaagctaaa gaaggaagca
    34681 aagtaggatt ggcctttgag ccagaagata tccatgtcat gcgtttcaac gagtctgaag
    34741 aagaattcga tgcacgtctt gacagctacg aagaataggg gggacaagaa tgaaaacaat
    34801 gcgcaggatc tattccgtcc cttatatttt gtggctcggt ttattcgtga tcgcgcctgt
    34861 attaatgatc atttatcaat ctttttttga tatgaatggg caattcacgc tgaataatta
    34921 tctatcttat tttacgtcgg ggacatcgct ttctatgacg atcaattctg tatggtatgc
    34981 atttttgatc acgtttttca cgctgatcat cagttacccg actgcttatt ttttaacaaa
    35041 actgaagcac aagcaattat ggctgatgct gattattttg cctacatggg tgaatctgct
    35101 gcttaaagct tacgcattta ttgggatttt cagtgtgcac ggcagcgtga atcatttctt
    35161 tgattttatc gggatcgggc cgaaacagat attattcaca gacttcagtt ttctgttcgt
    35221 ggcaacgtat atcgagattc catttatgat tatgccaatt ttcaatgcat tggaagagat
    35281 gaatccttct ttgatcagtg ctagtagaga tttaggcgcg aatagctttg agaccttccg
    35341 acgagtgatt tttccgttat cattaaatgg tgtgaaaagt ggtgtccaag cagtatttat
    35401 tccttctctt tctttattca tgctgactcg tctgattggc ggaaatcggg tgatcacttt
    35461 aggaacggct atcgaacagc atttcttggt tacacaaaac tgggggatgg gttctacaat
    35521 cggtgtgatt ttaatcattg ctatgttcat cgtgatgctc ctgacaggcg agaagaaaaa
    35581 aggaggtcgc tcataaatga aaaaatttaa atggtcgtat ctttatttag ggatcgtctt
    35641 tttattccta tatgcaccga tcttctatct gattttttat tccttcaatg aaggaggaac
    35701 gatgaacagc tttacaggat ttacttggga aaattatgaa gctgtttttg aagatacacg
    35761 tttgatcatt atcgtattga atacgttcat gctggctttc ttatctgcat tgatcgccac
    35821 gatcatcggg acatttgggg ctatgggaat ctattacacg aaaaaacgac gtgcacggac
    35881 agcgttgttg agcatgaata acatcttatt agtctctcct gatgttatta tcggtgctag
    35941 tttcttgatt ttctttacac tactaggatt cggattaggt tttcagagtg tattgctttc
    36001 ccatattgct ttcagcattc caatcgtcgt cttgatggtc ttgccaaaat tgcaggaaat
    36061 gaacgattca atggtcgatg cggctagaga ccttggtgct aataatttcc aagtagtcaa
    36121 aaatatcatt ttgccttatc tttctccagg aatcatagct ggatatttta tggcatttac
    36181 ctattctctt gatgattttg cggttacatt tttcgtcaca ggaaatggct ttacgacgct
    36241 ctcagtagag atctattctc gtgcaagaca aggaatcagt ttagaaatca atgcgctaag
    36301 tgcgttagtt ttcttgttct ccatgatatt ggtcattgga tactacttta ttagtaaaga
    36361 aaaccgtcca aaacggctga agaaaaacag aagagtacgc gagggggtgg cacggctgcg
    36421 atgaaaaaat tacaatctct gtttttaggt attctcatca ttattgcggt gctgctgctt
    36481 ggtgttcgtc aacttgaaaa aacaagtggc atgagtggag caaaggtact taccatctat
    36541 aactggggag attatatcga tcctagtttg atcacgaaat ttgaaaaaga atatggatac
    36601 aaagtgaatt atgaaacatt tgattctaat gaagcgatgt tcacaaaaat ccagcaaggt
    36661 gggacaaact acgacatcgc tataccaagt gaatatatga tccaaaaaat gatcaaagaa
    36721 aaactagtac ttccattaga tcattcgaag atcaaaggat taaataatat tgatccacga
    36781 tttttagact tggattttga tccagataat cagtattcga ttccatattt ttggggaact
    36841 ttagggatcg tatataacga taaattcttt gatgcaaatc aaattaagca ttgggatgat
    36901 ttgtggaagc cggaattaaa agacagtttg atgctgattg acggtgcacg agaagttatg
    36961 gggctttctt tgaatagttt aggctattct ttgaatagta agaatatggc acagctgaca
    37021 gaagcagcaa ataaattgaa tcatttaaca ccaaatgtca aagcaatcgt ggcagatgag
    37081 atcaaaatgt atatgatcaa tgaagaagcc tccgtggcag tgacattttc tggcgaagct
    37141 gccgatatga tgtgggaaaa tgaacattta cattatgtga tcccaccaga aggatccaat
    37201 ctttggtttg ataatatcgt tatgccaaaa accgtgaaaa ataaagaagg tgcctatgat
    37261 ttcatcaact ttatgctaga accggaaaat gcggcacaaa atgcagagta tattggttat
    37321 tctacaccaa atgaagaagc aaaagcgtta ttgccaaaag acatttcgtc ggacgaacaa
    37381 ttctatccat ctgatgacac gatctcccat cttgaagtct acaaagatct aggatcgaaa
    37441 tacctaggca tctataatga cctattctta gaattcaaga tgtataggag ataatggcaa
    37501 aactcagtaa gaatggggac ttatcaacgt tctaaagagc taaaaatcgt cctttttcta
    37561 tctgagtttt ccaatcttct accaaaagcg gatgaaacgc cgcttttttt gtgttatttt
    37621 attttttcta ccacgttttt ctaccagaga aaagtggaca aaaccattgt aaaatagggc
    37681 ccgagacggg ctagtaaatc taagattgaa aaagttacag aaggataaag taattggaaa
    37741 ctatgcaaat atacttaacc cttctgtaat ctattaataa aactaatata tgaatccagt
    37801 tcaatatttg tagctatgaa aaaacataaa aatcatatta gataaaaact aaattggtag
    37861 ataaattatt ttgtcagata tctgaggcaa atattcgact atttgtgttt caaagtaaaa
    37921 attggaatac aaatatagaa tagacatatt gcagccgata gtttattatg cttttagaca
    37981 cattaaactg gaggaaaaaa tgaaaaagag cgctaaagat cgagtttttg atgagctgat
    38041 acgactaaca gggaatatgt cttctgtcac tgccgagaaa atttcgaaga atctaggtat
    38101 ctctcgtcag aatgccagcc attatttgac gagattagtg gaagataaaa aggtcgagaa
    38161 attaccggga aagcctgttt tgtggaagcc tttagatgaa tatgcggttg tagataatac
    38221 ggagcaaata aacgaagcct ttcattctgt agttggtcat gacggaagtc tgagagaggt
    38281 cattcaaaaa tgtatagccg ctgtcaaata tccgcctaac ggactcagtg tattaataaa
    38341 tggagccacg ggtgttggaa aaagtttttt agccacaaaa atttttgaat acgctatcca
    38401 tgagcaaatt attgaaaaag atgcaccatt tgctatctta aactgtgccg attatgcgga
    38461 caatcccgaa ctgctttcgg caacactgtt tggatacaag aagggggcct ttacgggggc
    38521 tgaaaaggat accgatggtt tattggcaac ggccaacaac ggctatcttt ttctcgatga
    38581 ggtccatcgc ctatcgaaag aaaatcaaga aaagctcttt ctatttattg atactggaaa
    38641 ctatcgtcca gtcggtgaaa atgtaaattg gcattctgct aaggttcgtt ttattttcgc
    38701 cacgacggaa aaaggtgaaa attatttatt agacaccttt gatcggcgta ttcaaattag
    38761 tgttatgttg ccaacttttg aagatcgccc cattcgagaa cgattagagc tgattcaatt
    38821 attttttcaa aatgaagcgg atgttttaca aaaagatatt attgtttcaa gagaagcctt
    38881 gtctttaatg ttatctcatc cctttagtgg gaatattggt aagctgaaga atatcattaa
    38941 aatcagttgt gcggatgtgt atagtcgaac acaagaagaa ccattaacga ttgggaaaaa
    39001 tgagatcttg attcagttaa atatgctgga atccgaagta gaaagcttgc cgattgctag
    39061 tttattgata aagaaaaatc aaaagataaa aacggatcga atcaaaccgt ttgattatga
    39121 ttcgattttg gaaaaaatta ctcagctgat aaatgaaggt aatttcaatg aaaatttttc
    39181 ttcgataaag aatgaagtac agcatttggt gcatcacatt gataaggatt cttttttatc
    39241 aatgagtaga aaattgatga ttcagatgtt tagaaaggtc tgggtatccg ttttaggaaa
    39301 aacgtacgga ttgacgacga gtggacctgt tgcagaaatt attagcagat cctatgtcat
    39361 ttccccaagc tataagacag acttaaccgg gataaatcgt acattagctg caaatctacc
    39421 tcggacgact tacttatgta atcagttctt ggagcattta ccaccgctct cagaaaagga
    39481 cgagaacttt ctaagactgc ttattgtcgt gactttatct gattatgtag atgaatccat
    39541 cgagctgaag ggcctgttgc ttgctcacgg agatagtacg gctagcagca ttcaagcagt
    39601 agtgaatcaa ttatgcggca attatgtttt tgaagcgttg gatatgccag tagagagtaa
    39661 tgtgtcagag attgtcgaat cgacaaggaa tttcgtgaaa cggcaagtga atacagatag
    39721 tctagtgctg atcgtagata tgggctcttt aaatcaaatt tacacacaga tcaagaacga
    39781 tttaaaaggg gagttacttc ttctgaataa tttgacgact tccattgcac ttgatattgg
    39841 cttgaagatg acgatgaatg cgccatttaa atagattgcg gaggatgcag cagacaagta
    39901 ttcaatcaat gttcaatact ttgaaggctt ttctcaaagt agcaacatta tcatttcctg
    39961 tatgtcgggt ttggggattt cggacaaatt gaaagatgtt tttcgtcatc atttatcgga
    40021 tgaatccatc gaagttttca cgaaggacta cagagaacta cgtgaattaa tcgaccaaaa
    40081 tgatgaaact tattttgaca agacaaaatt ggtcatcacg acttcggatc ttccacgttc
    40141 cttttcaatt cctaatatta atgtttatga catcttagac ggtgatggag cagccaattt
    40201 ggagcatatt ttgagtcctg aaatgaagcc ggcagaattt aaccgcttga ttcaggagct
    40261 agtaaaattc ttttcgttgg aaggaattgc ggaccgtttg aactttctga acccaacgat
    40321 cgtgatgagt gaagtggaaa cagtcatttc taaatatgaa aattactatc atttcacttt
    40381 aaatggccgt gtaaaactaa atctctatat gcatacggca ttgatgatgg aacgtttatt
    40441 cttggctcgg acagaaaaat ctgaagaacc gaattatgat ttgaaaccag atgaagatga
    40501 attttattat gtttctagaa gtatttttca atcaatggag atgaaatata attttaaagt
    40561 caatgcttat gagctttcgc tgctctatga attattagga ccgtatattc aaaaataaag
    40621 tgtctaaaaa agtgtctaat ctgttttttt taggttagac acttttttta atgtgaatta
    40681 acgtgattaa accaatgttt ttaaaagttg gcacaggaat tgcttatata aaagtgaaca
    40741 gaaagaaagg aattaaccat atgaagaaaa ttctaatagc gactcacggt cacttagctt
    40801 caggctttct tagcagtatt caattactga cggggaaggt tcaagagatt acggtgatta
    40861 atgcttatgt ggatgagtgt gattttgaac aggagttgaa tgtatttgta aaccaggtga
    40921 acgagcagga tcagattttt gtttttaccg atttatttgg cggaagtgtc aatcagaaga
    40981 ttaccaaaac ctttttggag aaagagattg ctgtaactat tttgtctggc tttaatctgc
    41041 caattctgct cgaaatagta ttggcaacca aggaattaac aaaggaggat attcaggaac
    41101 tggtaaataa atgccaacag gaattaaaga tgaccattct tgatgaggta catgtagtaa
    41161 atgaagagga agattttttt taggaggaag aaaaatgatt aaagcagtgc gaattgatga
    41221 acgattaatt catggacaag tagcgatgac ttggacaaag agtctaaatt taaccgggtt
    41281 agtagtagca agcgatgagg cggctaccaa cgatattcaa aagatgacat tgaagatggc
    41341 tgcaccatcg aatgtcaaag tcattattaa aacagttgat gacgcgattc atttgttaaa
    41401 agatccgcgg gcgaaggaca tgcgcttgat ggttttagtc cctaatgtca aagatgcggt
    41461 aaaagttgcg aaagctttcc caacggaaat cgaattaatg aacgtgggga atgctgggaa
    41521 aatgacaccg ccatcggaag ataagaaaac cttatcaaag gaagtgatgt taacgacgtc
    41581 agaaattcaa gacttaaaag aattagttga actttatcca gataccgttt ttcagcctac
    41641 accggcgatg gaaaaacgtt cagctatctc tattttgagt gatttttaga aaaaggaagg
    41701 agaaaaatta tgtatgatgc atttgtagtc gctttggccg tttttattgg agttgctgga
    41761 catgaaatgt tcgggatgtc catgctgagt cgtccgattg ttgttgctcc gttagcagga
    41821 gcgttgatgg gggatttgca gacggggttg attgtcgggg cgtcactaga agccatcttt
    41881 atgggtgtcg tcaatatcgg aatctctagt acggcggagc cttctttggc tgctgggctg
    41941 gctacagcgt tcgcaatcaa aatgggagga aacgtggatg cgattattcc cattgtcttt
    42001 ccactagcag tattaggatt acagttaatg aatatgattt tttcttttgt atgtggaccg
    42061 atggctgcta aatttttcaa atttgcaaaa tctggtgatc aaaaaggaat catcaatctg
    42121 cacttcttta tgtggtttgt ccactatgcg ctttatgctc ttattccatt ctttgccgtt
    42181 ttatttggag cagatgttgt caaaagcgtc ttggataata ttccagaggt catcatgaac
    42241 ggattaacag tcgctggaaa tctgctgccg gcagttggga tggcgatgct gttacggatg
    42301 ctttggagta aaaatattgc tgtgtggttt ttctttgggg ccgttgttat ggcgtatctc
    42361 gagttaccat tgattgcaat tgccgcagtt ggaacgatta ttgcaattgt tatagcccaa
    42421 agagatctac aactaaaaaa tatatctgtt catgcagttc aaagcgatgc tgctgtaaat
    42481 gcaacgcttg accaagaaga ggaggatttc tttcaatgaa attagctgat ggaatttcta
    42541 aagatgagaa aaaattggtt cggaagatgt tttggcgctc gatgactatg tatgcttcgg
    42601 tgaatccggt gacgatgggc ggcggtggtt tcgcctattc tatgatgccc ttcattaatc
    42661 atttctacaa aagtgaagaa gatcgagctg cggcgctgga acgtcataca cagtattttt
    42721 cgacgaccat tccttgcgcg tcctttgtca tggggattgc ggcatcgatg gagaaggaaa
    42781 attctgaaaa agctggtttt gacgtggaaa gtatcaacgc gatcaaaacg agtttgatgg
    42841 gaccattggc tggaattggc gattcattgt tctggggagt ttggcgtgtc gttaccgcgg
    42901 gactagcgat caacttagcc aatgccggaa atattttggc accaattatc tttttagtta
    42961 tgttcaatat acctaattac ttgatgcgat actatggcgg cgttttaggc tattcattag
    43021 gctctaaata tattgaaaag ctgtatgcga acgggttaat tgaaattctg acaaaagctg
    43081 ctagtatctt gggtctgatc atggtaggtg caatgacgat cagtatggtc aaattcaata
    43141 ccgttttgag cttttcaatg aagggcgaag aactaatgaa tgtccaagaa atcctcgata
    43201 gtatttttaa aggaattgta ccgttattgg taacattgag ctgtttcaaa ttactagata
    43261 aaaaagtcgg cgtcatcaaa attatttttg gaatggtcgt cttgggaatt ttattgtcat
    43321 tacttggaat cgcatagtag gaagctgcag ctactaggct gcagtttttt tagcaaggag
    43381 ggaaaagatg actattaatg aagtaattta ttcacccaaa cacccatttt ttaatgctga
    43441 gttaaacgac ggaaatgtga ctacttcaaa cgaatacagc tactatcgga atattcctga
    43501 tgtaaaggga ttgaccttcg atgtaagtaa tcaaaagaca ttagcaaacc ttgatgtttg
    43561 cgggacgatt caaacgttga ccttttatcg agaaaattta ctaactgagg aaaaaccagg
    43621 tgtttgggtc aataaacagc tctctcaaac caattcgtta tccttaaagg ttgaagtaaa
    43681 cgggattgtc taccatttgt cagaatcgga tcatcgtatt gaagtcgatg tgattcaaga
    43741 ctgtttgcct agatatattc atcattattc gacgtttaag gtcattgtag tatcctttgc
    43801 tcccatactg gctaataagc gtttaagtat gcttgttcaa caggtttaca ttgtgaatga
    43861 aacacacgaa agccttcaag tttcagtgaa agatgtgcct tcgtatcaag agaagtattc
    43921 ggatcagcaa aatgtgttga tttctcaaaa aggaaataag gacacgatcg cacaacagga
    43981 agtggcaagc ttcgccattg cttttgtgga cccaaatgct tttgaagaag taatgacttt
    44041 tcaggaatct gatatcgaaa aatggctgaa ggaaacattg aactattttg ctcagctatt
    44101 tgggcagctg tcaatgccaa acgatagagt tgtccatctc tatcgtcgtg cactttatca
    44161 aagcttttct agtttcggta tgaatcgaca gggacaggtc gtcggcagca attggggcag
    44221 ctatcctgct actaatcgaa tttggaataa ggatatgtat tacgcatcat tgccattcac
    44281 catgtttgaa ccggagttat gtcaaaaaac ggttctatgg tttgatcaat atggcgttaa
    44341 gtttcctggt tcaaaattta caggaggtat caatcattct ctgagtaatt cgttatccag
    44401 tgcgttactg gctagtcttt attttgaaca tactggggat acaaaattct ttgagcggtg
    44461 tccgcaggtt ctactgaatg cttcaagaat tattgaggac atattagctc aacgtcggtc
    44521 aggggaacca atgctttttc catctgtttg gttatcagac gcctttgcac ttggaaagta
    44581 tcataccggc agcaatcttt gtctttggaa ggcctgtatt ggacttgcag aaatttttga
    44641 agccctagaa caactgagtc ttagcaagcg atacaaaaac attgcctgta aactaaagga
    44701 atcaatttgt aaacaaatga ctatcgatgg tccttttggt gagcaatttt tggagggaat
    44761 cggtgatgag gagaaaacaa cctattcagt gaagcactat caaaaaccga ttcttgagca
    44821 agggttaatc tttttaagtg atgtgattgt ggatggaaaa attaatttat tgatgcatga
    44881 tggagaagaa tcagacacta cgttgatgcc tttttatcaa tttttagata ataaagatca
    44941 tttatatcag aacaccatgg ccttttctgc tagttctttc aatccaacct atagcgagga
    45001 aatcaaaggc atcacttggg gattagaatc cggcgccact tttcctggct ttatcactgt
    45061 tttaatgagc gagctgcaca accctcaagc tttcgtaacc cgattagaag aactaatttg
    45121 tttggcagat ttagacggtt cgtggtggtg gtggccgtat cgcttagagg ctcaacaagg
    45181 agaagtcgtg cgagactttg gctgcggcaa atgcggctgg gcatcgggtc tttttgtcag
    45241 cctaatgatc agtcaatatt tcggattgaa attcaaaaaa ggagtattgc aaattgatcc
    45301 tgttgataat ttaacgtttt cttggaagaa tttgaagttc ggtcaagcct tcatttcgat
    45361 tgaatgtacg caatccgagt tgagtatttc aaatttgtca gaaaagccat tgaaaataag
    45421 tgatacgaaa aaaattcttc acgtagaaaa gggaaaaacg attcacattc aaaggagaaa
    45481 aagatgagta tactaaaaaa tattacccaa tttaaaaaaa tccagagtcg agcaatcact
    45541 cccgaaaatc caacaggcga gaaaggtaaa gctgcgatgt tgccaagcga gctaggacca
    45601 agccgcaaag gtcgtggcgc aattccgctg acttctggtg aagaaactgt gattgcggat
    45661 attactggaa cgggaataat tcgtcatatg tggatgacga ttcgtgatcg aacagaattt
    45721 ggcagttttg ttttacgtga tttaatctta agaatttatt gggatgggtc ggatagccct
    45781 gctgttgaaa gtcctttagg ggatttcttt tgtaatgggt ttggagagcg ttgcgaggtg
    45841 aactcgttgc cgatagtggt taatcctaca gggggcttta attcctattt cgaaatgcct
    45901 ttccaaacat ccgctaaaat tacgatcgag aatttacatc cgaaggatat gtcctcattt
    45961 ttttatacga taaattacgc tttggtggac gaattggaag aaaatagcct atattttcac
    46021 gcaaattggc atcgggaacg cattaccaaa ttacaggagg attataagtt aattgatcga
    46081 ttaaagggtc aaggctatta cgtgggtact taccttgcgc ttacctgcct agaacgttat
    46141 tggtggggcg aaggcgagtt taaattctac attgatgggg atagtgagta tccgaccgta
    46201 acctcaacag gttcagaaga ttattttggc ggggcgtggg cctttcatga gtacgatgag
    46261 ttcggacgtc cacatgcgaa aaactattca acagcctttt tagggtatcc ttaccaatca
    46321 aatagagatg ccacaagaga tcgttttgaa acgggaaaat tgaatgccgt tcatgccttt
    46381 ggtgacgacg caatgccacg acatggtctt tatcgttggc atcttcaaga ccccatctgt
    46441 tttaccgaag atttatcggt cactttacaa caaatcggca acgatgatgt tcgcttattt
    46501 gagcggcaag acgatgttgc tagtgtcgct tactggtatt caacagaagc ttcaggaaat
    46561 actaaacgtc atttcactga tcgagaacgc ttgccgcgtt aatgaagtgt ttttcacata
    46621 ttcgaaggga ggaacctatg atgatatatg gggcaattga agctggaggg actaaatttg
    46681 tctgtgcagt cggtactgag gaattaacga ttttggaaag agtcagtttt cccacaacaa
    46741 ctccagttga aacaatgaag caggtcattg atttttttaa accttttaag gaacaattgg
    46801 caggcatcgg ggtcggctgc tttggtccaa ttgatattca atgcaaatca acgagtttcg
    46861 gtcatatcac atcaacacca aaacttgctt ggcaaaattt caattttgta ggaacgctaa
    46921 agcaatattt caatattccg attgaatgga caacagatgt caatgcggct tgctatggtg
    46981 aatatgttgc tggttctggc aaagggttag caggtgttgc atacttcact gtgggaacag
    47041 gtatcggagg tggtgccttg ttcaatggga ctttcgtgga agggttcagt catccggaaa
    47101 tggggcatat gttgatcaag cgacatccaa aggacaattt ttcaggaaac tgtcccttcc
    47161 accaagattg tttagagggg atggcagcag gaccagcgat tgaaaaacgg ttgggtgtaa
    47221 aaggccagaa tttattggct gacgattcgt tttggcagat tgaagccttt taccttgccc
    47281 aatgtgcata caatacaaca ctgatgtttt cgccagatag aattattttt ggcggtggtg
    47341 taatgaaaca agaacacatg aagaaaaaag ttcaggataa atttgttgag cttataaatg
    47401 gatatgttga aataccacca attgatagct acattattac gcccgaatta ggtgataatg
    47461 ccggaataat cggcggatta gcattggcca gaaaagcggt aagaaataag caaccataat
    47521 taatgaatgt ttcattgata ctcatgtcaa aagtatttaa ccgagtaatc aaatgatcaa
    47581 atgaagttgg ataaagcgaa aatgaattca aataaaaagg ctctaagtca aatcgtgttg
    47641 ttttcattaa atcactgaaa ggaaatgctt ctgcgtttcc ttttttcttt ttgttgtgcg
    47701 ctgtatacct tgaggatatt cactggtggc gtacacttaa atagtaatat attgtgttac
    47761 tttatgtctg tggtaatagt tatagttgct taccaatgta tgttcatttt tcgaaatgtt
    47821 gacttcataa tccttggctt aatgtttgaa tttttttggt gatagatttt ttcagccgta
    47881 taaaataaat cgtaattcta ttatagagag ttgtgattat aaaaagatag caagaaaaaa
    47941 aaagaaagtt tctatacatt ttaaatattt tattcctatt tctattgcta aaaaagtaga
    48001 aattctatat ttacctttaa ctctcgcttc tcttgcaaat gtagttagca ttttaatcga
    48061 taataaactc aggtagtta
//
LOCUS       DANJMI010000021        44673 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000021 DANJMI010000000
VERSION     DANJMI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.021"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            178..1605
                     /locus_tag="PWU81_001369"
     CDS             178..1605
                     /locus_tag="PWU81_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1110082.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1697..2062)
                     /locus_tag="PWU81_001370"
     CDS             complement(1697..2062)
                     /locus_tag="PWU81_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL1110083.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2059..2424)
                     /locus_tag="PWU81_001371"
     CDS             complement(2059..2424)
                     /locus_tag="PWU81_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL1110084.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2616..2864)
                     /locus_tag="PWU81_001372"
     CDS             complement(2616..2864)
                     /locus_tag="PWU81_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110085.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2863..2991
                     /locus_tag="PWU81_001373"
     CDS             2863..2991
                     /locus_tag="PWU81_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HDL1110086.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3048..4742
                     /locus_tag="PWU81_001374"
     CDS             3048..4742
                     /locus_tag="PWU81_001374"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDL1110087.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4746..4865
                     /locus_tag="PWU81_001375"
                     /pseudo
     CDS             4746..4865
                     /locus_tag="PWU81_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4842..5015
                     /locus_tag="PWU81_001376"
     CDS             4842..5015
                     /locus_tag="PWU81_001376"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110088.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5393..6526
                     /locus_tag="PWU81_001377"
     CDS             5393..6526
                     /locus_tag="PWU81_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL1110089.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6557..6877
                     /locus_tag="PWU81_001378"
     CDS             6557..6877
                     /locus_tag="PWU81_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HDL1110090.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6906..7370)
                     /locus_tag="PWU81_001379"
     CDS             complement(6906..7370)
                     /locus_tag="PWU81_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1110091.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7502..8755)
                     /locus_tag="PWU81_001380"
     CDS             complement(7502..8755)
                     /locus_tag="PWU81_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1110092.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8784..9101)
                     /locus_tag="PWU81_001381"
     CDS             complement(8784..9101)
                     /locus_tag="PWU81_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL1110093.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9127..9447)
                     /locus_tag="PWU81_001382"
     CDS             complement(9127..9447)
                     /locus_tag="PWU81_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1110094.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9911..11326)
                     /locus_tag="PWU81_001383"
     CDS             complement(9911..11326)
                     /locus_tag="PWU81_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL1110095.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11343..12659)
                     /locus_tag="PWU81_001384"
     CDS             complement(11343..12659)
                     /locus_tag="PWU81_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1110096.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12809..13507)
                     /locus_tag="PWU81_001385"
     CDS             complement(12809..13507)
                     /locus_tag="PWU81_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDL1110097.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13796..14752)
                     /locus_tag="PWU81_001386"
     CDS             complement(13796..14752)
                     /locus_tag="PWU81_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL1110098.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14865..15698)
                     /gene="msrA"
                     /locus_tag="PWU81_001387"
     CDS             complement(14865..15698)
                     /gene="msrA"
                     /locus_tag="PWU81_001387"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL1110099.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15873..17507)
                     /locus_tag="PWU81_001388"
     CDS             complement(15873..17507)
                     /locus_tag="PWU81_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110100.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17494..19362)
                     /locus_tag="PWU81_001389"
     CDS             complement(17494..19362)
                     /locus_tag="PWU81_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110101.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVL
                     YYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIK
                     FVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNN
                     DITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPIS
                     LLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAK
                     VIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKY
                     KAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENK
                     IEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            19328..20965
                     /locus_tag="PWU81_001390"
     CDS             19328..20965
                     /locus_tag="PWU81_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110102.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(21110..21850)
                     /gene="truA"
                     /locus_tag="PWU81_001391"
     CDS             complement(21110..21850)
                     /gene="truA"
                     /locus_tag="PWU81_001391"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL1110103.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(22041..22676)
                     /locus_tag="PWU81_001392"
     CDS             complement(22041..22676)
                     /locus_tag="PWU81_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL1110104.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(22823..24064)
                     /gene="sstT"
                     /locus_tag="PWU81_001393"
     CDS             complement(22823..24064)
                     /gene="sstT"
                     /locus_tag="PWU81_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL1110105.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            24262..25260
                     /locus_tag="PWU81_001394"
     CDS             24262..25260
                     /locus_tag="PWU81_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110106.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            25273..27291
                     /locus_tag="PWU81_001395"
     CDS             25273..27291
                     /locus_tag="PWU81_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL1110107.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(27351..28067)
                     /locus_tag="PWU81_001396"
     CDS             complement(27351..28067)
                     /locus_tag="PWU81_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL1110108.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(28294..28836)
                     /locus_tag="PWU81_001397"
     CDS             complement(28294..28836)
                     /locus_tag="PWU81_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL1110109.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(28841..29068)
                     /locus_tag="PWU81_001398"
     CDS             complement(28841..29068)
                     /locus_tag="PWU81_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL1110110.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(29147..31027)
                     /locus_tag="PWU81_001399"
     CDS             complement(29147..31027)
                     /locus_tag="PWU81_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL1110111.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(31216..31830)
                     /locus_tag="PWU81_001400"
     CDS             complement(31216..31830)
                     /locus_tag="PWU81_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1110112.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            31972..32532
                     /locus_tag="PWU81_001401"
     CDS             31972..32532
                     /locus_tag="PWU81_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1110113.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(32837..33151)
                     /locus_tag="PWU81_001402"
                     /pseudo
     CDS             complement(32837..33151)
                     /locus_tag="PWU81_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(33405..35040)
                     /locus_tag="PWU81_001403"
                     /pseudo
     CDS             complement(33405..35040)
                     /locus_tag="PWU81_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35479..36348)
                     /locus_tag="PWU81_001404"
     CDS             complement(35479..36348)
                     /locus_tag="PWU81_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL1110114.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(36368..37489)
                     /locus_tag="PWU81_001405"
     CDS             complement(36368..37489)
                     /locus_tag="PWU81_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL1110115.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(37491..37886)
                     /locus_tag="PWU81_001406"
     CDS             complement(37491..37886)
                     /locus_tag="PWU81_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL1110116.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(37905..38711)
                     /locus_tag="PWU81_001407"
     CDS             complement(37905..38711)
                     /locus_tag="PWU81_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL1110117.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(38704..39492)
                     /locus_tag="PWU81_001408"
     CDS             complement(38704..39492)
                     /locus_tag="PWU81_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1110118.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(39506..39982)
                     /locus_tag="PWU81_001409"
     CDS             complement(39506..39982)
                     /locus_tag="PWU81_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1110119.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(40054..42711)
                     /locus_tag="PWU81_001410"
     CDS             complement(40054..42711)
                     /locus_tag="PWU81_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL1110120.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNIRKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(42730..43134)
                     /locus_tag="PWU81_001411"
     CDS             complement(42730..43134)
                     /locus_tag="PWU81_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HDL1110121.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(43366..43623)
                     /locus_tag="PWU81_001412"
     CDS             complement(43366..43623)
                     /locus_tag="PWU81_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDL1110122.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(43748..44080)
                     /locus_tag="PWU81_001413"
     CDS             complement(43748..44080)
                     /locus_tag="PWU81_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110123.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT        14139 a         8723 c         7037 g        14774 t
ORIGIN      
        1 taaaaattcg caatttattt tttaatagaa ttatctgttt tccaaatttt ataacataaa
       61 aaaccttttt taacggcatt tatatgcttt ttcagaaatt aactgatgat ttcaaaaaga
      121 aaacatgtca taatatttcc catattgtgc aaaaaaatac aaggcggagg ctctactatg
      181 attgaagaat atattgagaa aaatatttta agacagctgt ttttatgtgg gcaattctat
      241 gtaaataaag aagtcaatct agaaaagctc tccaatctcc tgcatgtttg caaaaccact
      301 ttactcaatg atatcaataa cataaaaaaa gaatttgaag aacagatcgc ttatacacat
      361 cgggaaaagg accgttacac tttatatttt tcagaacata tacctcgatg caagatcatg
      421 cagcaattgt ctcaaaattc gttgttttta aaaacttgtc tgctttattt ggaagaagat
      481 gaacctgatt atcttcaatt aactgaatgt gaattcatct ctgtcagcaa agcttattca
      541 ttaaaaaaac aagtattagc ttattttaat gactgcggga tcgaaatcga ccgctactcc
      601 cctcgattca ctgaaatgga aagacgtctg ctgcttctta atgtgtcgta tcgtttaggc
      661 ggttttaatt cttgggagct acctgaatcg ttttttgaac gtgccgatcg tttcattgaa
      721 tccgtaacgg aaaatagtgg cagattttat gataaagaaa ataaagaaat cttatcgatt
      781 ggttttgcta tcagtttcct acgccagcaa gtgtgtgctg tgacaataga ttcaaaattt
      841 atcgaagaaa tcaaaaaacg tcctatttac aattatgtcg aatcagcatg ggaaaacact
      901 gatttccaaa cctattataa aaaagaagaa ttcgccttta ttttgacact attcaatctt
      961 tgtaattatg gtttccattc ttatcagttg attgcggaag actttcaaca gctacaccaa
     1021 gtgtttattg acaatactcc cgaaataaaa gaactagttg ctacctttga atctcacttc
     1081 aatcaagaat tattcggaaa ccaaccgttc gaacgtgcgc tgattcatct gatgcgttcg
     1141 gcttgggata actatcaatt attcatgcca gaaaaatttt atcttttaaa tgaggaacag
     1201 acaaatttat ataaagaagt ccagacaatt ttttccagct ggtccagcca gttaccttat
     1261 gatctacggt tgaatcctaa ttgcatgcgg gcttttgtga tcgaattgag cggaattctc
     1321 cgtctgacaa aagaacatct gactatctat atcgtcacaa attcggatgt tcattatctg
     1381 atttatcggg aagccttgga agctgtaact actttcgatt ttcaagttgc accaacaatt
     1441 tattcaagta tatctgatat aaagaagtat gcgcagcagt cctcaaaccg ggtattatgt
     1501 gaacgtacat tatatacacc agatgcggtc caatatgaaa atattattcc tatttcgatc
     1561 aatacgatcg atcgagctat catttcagct gtgcaaaaca aataaggggc tgtgacaaaa
     1621 atcatgtcac agcctcttat ttgttgaaga tgcccatttc tgtaacaatc tcttgataca
     1681 cgtcaggctc acaacctcac ttattttgga catctacggg tccgatctct ttatttaaaa
     1741 aataagaaat gatggttcgg ataactacca acgccgctag ttggaaaata tcctgcaagg
     1801 taggtttgat aatcgattcg atgatcccag ctgaaacaag aatttccaaa cttagaagta
     1861 tatagcctcc caacatcttt ttaatagcat tgttctcgct gactcggtcg ctttgcactt
     1921 ttgtatggac aaataaatat ttcaaaaaat ccttgattga tagacaaaca ccccaaacca
     1981 ataaacagat agcaaagata tcaagtccca gtacaagttt ttcgaaatat ggaagaagaa
     2041 actgcatcaa ctctgtcgtc atcctacctc tcctcttctt tgtttttctt tgtcttctat
     2101 ttcctgattc aagaaataag aaatgactgt tcgaatgata ataataagtg caagcttcaa
     2161 aatatctgtc caagtcggct tgataactga ctcgatgata tcagcaacga ctagtacttc
     2221 caagcttaac aaaatataac ttgctagcag ttttcttgta tctgcattac gagtattacg
     2281 ttctgcatag tttttcttga catgagacca atcaaaaagt ttgaatagaa caagaacgat
     2341 cccgacaatt aagataccga tagaacaaat atttaaaata gcaatgatcc attctagaaa
     2401 ttcaattaaa tttgagcgaa acatatacgc tacctacttt cttttaattt gttcctattc
     2461 ttttattgta aaagaaagtt accttgttcg tctatttaaa agttgttcta atgataacgg
     2521 ttcgcttaaa tttttctttt tcactctccc atttttctcc ctccatccat aaatcgtgga
     2581 agtatccaaa acaacgtagc aagttaaaat aaggcttagt ccgtcatcag cttttcaaag
     2641 aagcactgtc tcggtgcttt ttttgtaccc aaatttctaa aaatagaacc atacacctcc
     2701 tcaaatcttt tcttcttacc taccctattt ttatttacag gaaaatcaga cacttcttct
     2761 tttttgtcaa aatttccttt ttcttctcgt cctatcattt ggcattctaa taaaaacgaa
     2821 ctatccttgg atatagaaag agaaaacaag gaggcgtttt tcatgagtaa aaaattaatc
     2881 aaagtcggca tcggtttagg tttgcttgcc cttggcgctg catatctagg aaaaaagaca
     2941 ggactattcg aagatgacag tcatttatat gatgaattcg aatcaatcta gcatcctatt
     3001 tagttgaatc actacggaca tacttggaag acattggagg aatcacaatg agaaaaagta
     3061 aagcaataat gattggtgcc ggtattgcca atatggcagc ggctgtttat ttgattcaag
     3121 aaggcaaatg gaaaggggat cagatcacct tttattcatt ggatgatcac ggttcaaatg
     3181 atggtgcacc gaccgacact gtagtggatg aatattggaa taaaaaccat ccattagaaa
     3241 accaaaaagg ctatattgct cgaggcggtc gcatgcttaa ttaccgtacc tatgtagatt
     3301 tgatggatct gttaagtcga ataccttctg ttactgagcc agggatgact gctgaagaag
     3361 atacgagaga ttttgatgca aaacaccgaa cctttgacaa agcacgtttg atggaaggtg
     3421 gcaaaggaat cattgatggc ggaaaactgg gattcaataa taaagaccgt attcttctga
     3481 ctaaactgat tgctatgcct gattcagaag aagaaaaact agacaatatc actatcgaag
     3541 aatatttcaa agatgatcca cacttttttg aaacaaactt ctggtttatg tgggaaacaa
     3601 catttgcttt cagaacacgt agttctgctc aagaattgcg tcgctatatg catcaaatga
     3661 tttatgaatt cactcaaatc gaacatctag tgggcgtcaa tcgaacacgt tacaatcaat
     3721 acgaaagtat tatgcttcct ttgatcgagt acttgaaaga acaaggatgc aaaatcattt
     3781 tgaaccgccg tgtcactgac tgggaattca aagaaacacc gatgcaagat gaaatcactg
     3841 tgactggttt gaaaattacc aatacactga ctcaagaaga agaatacgtt gtggtcgatg
     3901 aagatacagc agttattttc actaatgggt ccattacaga ttcagctact ttaggagatt
     3961 acgaaacacc agctgtagaa aatatggatt atggtgcagc ttctagtctc tggaaaaaag
     4021 ccagtgaacg tttctacaac ttgggaaatc ctgacaaatt ctttgctgat cgagatgcaa
     4081 gcgaatgggt cagctttacc ctgacgacga aaaaccattt gttgttgaat gaaatcaccc
     4141 gtatcacgac tcaagaacca ggaaatgcct tgaattcatt tatttcaacc acaccgatca
     4201 cgccgttagg ccaaaaagat gtcaatatgt ctattgtcgt gcatcatcaa ccgcatttta
     4261 cgtctcagaa accaaatgaa acagttattt ggggttattt cctttatcca agacgacgtg
     4321 gcgaatttgt ggataaacag tatattaaaa tgaatggaaa agaaatgcta gaagaactga
     4381 ttggacaact ttcaaaagta gatcctggac cagtgaatat tcgtgaaaaa gaaacagaaa
     4441 ttttcgacag cgtaataaat aatatccctg tctatatgcc ttatgcttcc gcgttgttca
     4501 ataatcgtgc caaaagcgat cgaccaaaag tcattcctaa acattcgacc aatctggcct
     4561 ttacaggaga atttgtcgag cagccttatc agatgatctt tactgaacaa agtgctgtac
     4621 gttctggaga aatcgctgct ttccattttg cgggtattcc tatgtcccgt cttgtcaaaa
     4681 caccgcgtta cgacaaagat attcctactc tagctcgtgc cgcaaaaaaa atgtttgaat
     4741 aatcaagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacaacc aggctaaaac
     4801 agcttcttat gtttttgatt tggttggact tattaccgca gatgcaagct tttgaacacc
     4861 gtttaccaag aggttatgaa aaagctgccc tgcatcaagt aataagagca gccaagctaa
     4921 aacagcttct tatgtttttg acttggttag acttattacc gcagatgcaa gcttttgaac
     4981 accgtttacc aagaggttat gaaaaaggcg tttagtttcg agtatgaaga aaaattttgt
     5041 gaaaatggtt cctcacgttt ttgcataaat ttgacttaat accgagaaac tgatcccttc
     5101 tgtcataacc tctttttatt tatgtctgtt tctttttgaa aacaactatt ttttcaacgt
     5161 ttactctctt gccaatccaa catcatctaa cggatacaac tatttcaatg attaccatta
     5221 cactaaaaaa tcggaaacac acctctccga acacaaatac ataagactat agaatgtttc
     5281 atttaagaaa aaaagcgttt cattatcaaa aaggtaagat ctcctttaaa atgaaattat
     5341 ctgtagtact aataatgcag acaattttat gaatctgaaa ggagcatctc caatgaaagc
     5401 agtcacttgg caaggaaaac agaaaatgga aatcagaaac gtagatgatc caaaaatatt
     5461 agaaccaaca gatgcaatca tccgtatcac agcaacagcc atttgcggct ccgatctgca
     5521 tctttaccat catggtgaag cagttttgga aaaagggtat gttgtaggac acgaaccaat
     5581 gggaatcgtt gaagaagtgg gatctcaagt tcaaacattg aaaaaagggc aacgtgtggt
     5641 tatccctttt aatattagtt gtggtcattg tcatttctgc ctgaatcaca tggaaagcca
     5701 atgtgatgaa tcaaatccag atcagttata tggcgggctt ttcggatttg gcaagttgaa
     5761 cggtaatcat tggggtggtc aagcggaata tctaagagtt ccttatgcag actctacttc
     5821 atttgttgta ccagataatg atcttcctga tgaaaaagtt ttatttctat cagatatttt
     5881 gcccacagct tattggagcg ttgaaaacgc aggagtaaaa aaaggcgata cagttgtgat
     5941 tctaggttct ggtccagtcg gtctattcgc tcaaaaattc gctgttatgg ctggagcaga
     6001 gcgcgtaatc gcagtagatc cagtacagca ccgtctagat aaagcggcta attacaatgg
     6061 ggttgaaact tatttcttag aagacacggc acaagctgga acagaattgt atgaactggc
     6121 taaaggtggc gcagatgtca ttatcgattg tgtaggaatg gacggattag agcctgtcaa
     6181 agaaaaagcg aaaaatttag taagcttaca aagcggcaca atctctccat tacagatggc
     6241 atcgcaagct gtcaaaaaat ttggaacagt ccaagtaact ggtgtttata tgactccagc
     6301 ttcttcttac ccgctgcaag aattttttat gcgggatatc gctgtcaagc atggacaggc
     6361 accaattatc catctaatgc caaaaattta cgcaatgatt gcagaaggac tattcgatcc
     6421 tacacaaatc attactcatt cgctgccact atcagaagca gcacaagcat atgagatctt
     6481 tgacaaaaaa gaagataaaa atataaaagt tgttttgaaa ccttgaattc agtaaaaaat
     6541 acaggtgatt ttctttatgc ccaaaatcaa acgaaatacc catgagatcg aaataggaaa
     6601 agaagaagat atcgaaatac gaggacgtcg cttccgcttc tatttccaaa atcgttacac
     6661 attgatctca ctggctgtcg atttattaac cgggattttc tatatttttg gaagccttgc
     6721 ttccttaact aaaatccccg atcgatatgg cacctatttt tatttggctg gtgctatttt
     6781 cttgacgatt cgtcctattc tcagaatcgt aaggaacatt tttatttacg acgaaaaaaa
     6841 agcaatgaag agaacaagca aaaaaaaaga ggcatgagaa tttttcaaat ctcatgcctc
     6901 ttttttcacc ctatcgtatc actttgatac agacgagcgg tcgtcccact ttcttccttg
     6961 accattgtca aaaatgtcca ctcgtatcgt tcataaaacg tggtatgatc tgtaattaag
     7021 taaagttttt cgtacccaag ttgatgcatt tcttttctga tttccgctaa tatccgcttg
     7081 gctatcccct gcttacgctt ttccggttca acaaacaggg cacaaagatt tggtgtcaga
     7141 tcagggcggt tatggaagtc gttttcaatg acgcccgctc ctccgacgat ggtttctttt
     7201 tcatctaaga ccacatacca ttgaggaata ctttccttat tagcgattcc agtacgaata
     7261 ctttcctcat acacaacagc aggaactccc cattttgatg aaaaccaatt cgcgctttgt
     7321 tttactaatt ctgggtgttc tcttaatttg atccagttat atgtaaccaa tgtttaaacc
     7381 tccgactaaa ataaaacgtt tacaaaaatt atacaagtct ttatgaaaat tagcaatgac
     7441 aacaatagtg atgtaaaaaa aaaaacggaa aacaatcact tgttctccgt tttttgatta
     7501 atcaagcatc taaagcttct tttcttcttt cttggatcgt agctaccttg acgaacggta
     7561 gatagatcaa aatagaaaga accaaattga cagcggctag tacaccacct gcaatacttt
     7621 ttgttgccaa tacaccgcca ataattggtg gagttaccca tggaggcata acggtagctg
     7681 ctggcactaa acctgtagaa gtcgcaaagt aagcaacact aactaagacc attggtgtca
     7741 aaataaatgg aatgaacatg atcgggttca acacgatagg taacccaaac atcgttggtt
     7801 cattaatatt gaagacacct ggcgcaatcg acaagtttgt tacaaccata tatggtttgt
     7861 ttttacgtcc tactaaataa atagctaaca gtaaacctaa agttgcacct gttcctccta
     7921 agttgacaaa gctgtcaaag aaaggtttgt tcacgataaa cggtgcgcta tgtccagctg
     7981 cgattgcatt aacgtttgct tcaatggctg gcgcattgat tgtttgcatc aaaggatcga
     8041 ccatattcgc tccgtgtaaa ccaaagaacc ataagaatgg tgtgataaat gcaagcagta
     8101 aggcagatgg ataagagttt gcaagtccca taaatggttc ttggaccact gtatagaaag
     8161 aacctacgat atcatcaaca ccgaatgcag caaaaattgc agcaactaaa gcaaacgcag
     8221 agatagtgat cattgctggt agtaatgaag caaatgcttt tgcaactgcc ggtggcacac
     8281 catcaggcat tttgatgatc aatttttcat ttccgcttaa acgtacaaag atttctgttg
     8341 aaataatccc aacaataata gcaataaaca aaccgtttga gctcatacct aatgctccgc
     8401 ctaaagcgaa gaaagagcct aaagaaatca cacctgcgga aacaccgtct tttcggtagc
     8461 cgttagctaa atgataagca agcaagaaag caatcaagac agaaagaatg gcaaatgtac
     8521 cgttccaagc agcagccccg aatcctttcc atgattcacc accaaaaatt gagttcatca
     8581 attcttggaa cccgggaatc ggtaagttat tgatcattac tgctaatgct cctaagatca
     8641 ttaacggcat actcaccata aatgagtccc gaatcgcaac taaatgccgt tgtgcaccaa
     8701 tttttgatgc tacaggtata aaatgtttct ccataaatgc aacaaattta tccataaatc
     8761 ttccctcttt tctttactgt agattacgcg ttcaatcgac gatacagatc aatgatctct
     8821 tttgctaaat ctgtaaaagc tatagatgtc atcagatgat cttgactgtg gaccgtcaaa
     8881 agcgtgactt caacgtgatt tccttgtgct tcttgtgtca gcatctctgt ttgagagtga
     8941 tgtgcttgga ctaaagattc ctctgcatct ttgatttttt catctgctaa agtgaaatca
     9001 ccttttttcg cagcagcaat cgcttccatt gcatcgcttt ttgcatttcc gccaaacatg
     9061 atcagaccca tgatggcttc tagatttttt tgatcttcca tttattgtat cctccgataa
     9121 tttttattat ttgtttatta gtttttctgc ttggtcgagt actttttcac cattcatcat
     9181 gccatagtca gccatattga ttacatctaa aggaatgcct ttaggtgcta gcttttcagc
     9241 aaattgattt tgcataaagc gtacttgcgg tcctaaaagt aaaacgtcta catttttaga
     9301 ttctagttga ttatccgcat ctgaagcaga tacagcaaag atgtctgctt ccattccttt
     9361 tgcttctgct gccttttgca tttttgtgac taggagactt gtactcattc cagctgaaca
     9421 tacaagcatg attgttttct tttccatttt aaacacgttc ctttctatac ttacacttgt
     9481 atataagcaa gtagcgtgcc aactttaaaa gcgctttaaa aagttgatat catcacattt
     9541 aaggtctata ccaagttgca tacacactaa aaaagaaagc gcacactaaa cacacaaatt
     9601 aatttttatt accttttttc ttttaacgtg cacaaaaaaa gttgtgacac aaaacttgtc
     9661 acaacttttt tcaaataaac ggtattcaaa agcttgcatc tgcggtaata agtccaacca
     9721 agttaaaaac ataagatgtt gtttttactt tgctattctt attacttgat gcaggacagc
     9781 tttttcacaa cctctcaata aacggtgttc aatcagctga ccctgggtat tagctcaaaa
     9841 cgctggattc acaacctctt ttatttcaaa taaccagtaa atacaatgaa catctacaaa
     9901 aaacttctat ttatttatag atttcgtcag gcaaatccaa ttctctctta tcaatcaaag
     9961 tctgatacca cgaagcagat tttttcaatg ttctgcttcg ttcatcgttc aattcgattc
    10021 gaactaatcc atatcgattc ttaaatgcat tcattggtga cacacagtca gtgaatgccc
    10081 ataacatata gccttcgcaa tttgcgcctt cttctgttgc tttgatcaag taatgcaaat
    10141 gctgtccgat aaattcgata cggtaatcat cttgcacgat tcctttctca tccatatact
    10201 gttcttcata ttctcttccc ataccgtttt ctgttatcag ccaagggata ttatgataat
    10261 tttcttttag atacatggct atatcataca tgattttcgg atagatttcc catcccctgg
    10321 aattattcat tttcttccca ggtaaatcaa agacttcata atatcgttca ggatggaaag
    10381 gtgtctcttc attccattga taacgagggg cgcgaacgcg tttaggataa tattgattga
    10441 ttccaaggaa atctactgta tgatgctgaa tcgtttctaa atcctctgtt gcaggatcga
    10501 aataaatcga ttgtttttga cacaactcca ggaactcttc tgtgtagtat ccttttacca
    10561 tcggatcaaa aaatactcga ttataaaaca aatcatacat ataagcagct ttttggtctt
    10621 ctggagaatt ggatcgagca tagaccattt caggatttaa aatacagcca atccttccac
    10681 cataatttcc gctgcggtaa atctctactg ctttagccgt tgctaaaact ttatgataat
    10741 tccacatcat ccactttttt gtgttttgtt catgcggcca gcgaacagca tccaaatagc
    10801 aacgcgtttg gggaacgatt ggttcgttga atgtaaacca gttttttacc ctatcaccgt
    10861 aacgatcaaa ggcaatttga gcatacttag cataaaaatc aactactttt cttgaagacc
    10921 agccatcata tttttccaaa agaaaagctg ggacttcata atgttccaaa caaatcatcg
    10981 gttctacgtc tgcttcgatc aaagagtcga tcatttcact tatatgctga gcatattctt
    11041 catctactat acagttctca taatcagtaa aaaaacgagc ccaattgata gaagtccgat
    11101 aatgtgtcag atggatggat ttcatcagat ccacatcctg ctggtattgt tccataaaat
    11161 tagttgctac ggctgggcca tacccattat gccaaacaaa atgttcattt ttataccagc
    11221 tatctataaa cgaatcctgt ccttcttttt ttcctttcca accttccgtt tgccaagcag
    11281 aagatgcagc accaagaata aaattttttg gaatagtaat tttcatgatt tcctcctgtt
    11341 ttttagatcc catcattttc cacacgattt gctactttta caaatggcag atagatcaaa
    11401 atagaaacaa taatacaaac aagttgcgta ataactgcac cgatatttcc tgctgttgcc
    11461 agataagcat tgacgatcgg cggcattggc caagggacca ttacaactgc tttcgccgca
    11521 aatcctaaag cggttgcaaa ataaccaatc gttccagtga taagaggcgt taagataaac
    11581 ggaatagcta aaattggatt cagcatgatc ggcataccaa aaataactgg ttcattgatg
    11641 ttgaagatcc caggagcaag ggataacttg gtgatttgtt tcatatcttc ccgttttcca
    11701 actaacaata ctgcaatcag taaaccgatc gtgactccag acccgcccat actcatatac
    11761 atatcccaaa atggcatagt aataatgttc ggtacttctt ttccagcttc aaaagcttcc
    11821 gtatttacag caattgccgc taaaagcaac ggttcccgta tcggcttcac catttgattt
    11881 ccatggatac caatcaccca aaagacttgc gcaataaaca tcaatagcaa aatacccggt
    11941 aacccttgta cgacaccttc taatgggcgt tgtacgacat tgtaaatgat atcataaagg
    12001 tacattcctg taagttcttt gatgataaat cccgcagccg cgatacccga tacagtcaga
    12061 atcgtcggga ataaagcaga aaaactagca gaaacattcg ttggcacggt atcaggcatt
    12121 ctcagctgta gtttcttctg tttcctcaac cagcaataaa gttcaataga aaggatggag
    12181 accaacattc ccaaaaataa ccctttcgta tctgtatatt gtttagccaa tacgttttct
    12241 actataacag tctgattttc attcactaat tcataaacag ttggattaac agataaataa
    12301 gacatcactg cgatgatacc agggaataca ccatccactt tattcagctt ggctaattcg
    12361 atccctatca gaaaaacagc atagatggtc aagaaactta gtgtgacata gctgatggca
    12421 gatgcgatag gctttaattc ttctaaaaaa cgcaagaaac ttatctgtgc taagccattt
    12481 tccgagtcaa agaccattgc tgaaaacaat gtgccaaaag cacccgtaat aattaccgga
    12541 atcaatgcag caaatgcgtt ttttatcacc atgatatacc ttaaattatt gattctcgtt
    12601 gccacgttcc ctaaaacatt ggctaattta tccataaaac tcattccaga tcccctcatt
    12661 cctgcttctt ctccttgacg tcatctcttt ttgtctatta gtcagcgctt acatttcgat
    12721 gaacagaaac tatttactcc tcttttttac caaatataaa acaaaaattc atctgatgta
    12781 tttatcataa aatacatcag atgaactgtc aattagtttc tttttctcct attattttct
    12841 gtaattctct ccgatttatt gccatatgga cagtcattgc atcaaacgca gcaagtgcat
    12901 tccgcctttc gatggcctga acgatctctg tatgaatatc gatcgtctcg attttcagat
    12961 gctggatatc ataattttta ttaaacaaag caattgattc attaatgatt gggataatat
    13021 gcagcaaggc tacgtttccg ctagcttctg caatcatcgt atgaaaatcg atgtctaatt
    13081 gaacatgttc ttgtgtgcct tctcgaaaag aagtttcaat cttttgcgtt aattttttca
    13141 tttggacgat ttgattcttc gttgcatatt ccgctgccaa acctgccatc ttaggctcca
    13201 ataaatatct cagttcaaat aagtctcgta tcatccgatc ctgatcttca atcaaagaaa
    13261 aaccgagtgg gtcattggca atccctcgct tatcactgac aaatgtcccc gctccttgcc
    13321 gaacttcaag gatattcctg gaagctagag cccgcacagc ttctcgcacc gtgcttcgcc
    13381 ccacctctaa ataatcggac aattcatatt cattcggcag tttatctcct acttgcaact
    13441 gcatttcctt taaatatcta aggatcttgt cagtagttac ttctactagg gttttatttt
    13501 cttccaaacc acatacctcc ttttcatctg atgtattttt tcgtaattat tataacacgt
    13561 ttgaatcatc tgatgatttt ttgtatacaa aattgattaa tccgttcata gacatacaaa
    13621 cgaagaagat atttaccata aaaaagacta ggataaaatt cactcctagc cttcgatcat
    13681 ttttattttg aacgacaaaa aatatgatgt tcaataacag acgatcttca ttcgattctt
    13741 gatgtcatct tttgtttttt ttaatatatg gataaaaagt ttgattacat cgtttctatt
    13801 ctagaatttg gtcgattctc aattcttctt ctggtgagaa atctaaatta tccatcattc
    13861 gaacactttc ttgtaactga cttaaccgac ttgctccgac cagtacactt gtaacagatt
    13921 tttgtcgcaa attccatgca agggccattt gcgctaatga ttgtcctcgt gatacggcga
    13981 ttgtttgcag tgctttgatt ttttccagag ttgatccaac ttgttcttct gaaaggaatg
    14041 gtatttcctt acgatgtgct ctagaatctt caggaatacc atgtagataa cgatttgtca
    14101 gcaacccttg agcaagcgga ctgaacgtga ttgcccctag cccttcttct tctaatactg
    14161 gagatagatc gtcttctagt agtgcacgag aaaacatatt gtagcgcatc tgatgaataa
    14221 tgaacggtgc ttcctttctt tttaaaattt cagtcatttt ttttgtatct tctccattat
    14281 agttggatat accaatatat aatgcttttc cttgtcttac aagttgcatc aaagcttctg
    14341 ctgtttcttc caaaggtgta tcagggtctg ggcgatgatg atagaagatg tcaacataat
    14401 ctagccccat tctttggaga ctttggtcac aacttgaaat gatggattta cgagagcccc
    14461 attccccata tggcccttcc cacatatgat aacctgcttt gctagaaata atcagttcat
    14521 cacggtaggc ttgaaaatct tctctaaaaa tacgcccaaa gtttttctct gcagctccag
    14581 cagggcctcc ataattattc gcaaggtcga aatgggtaat tcccatgtcg aatgcgcctc
    14641 ttaaaatctc tctttgatta tggataggat cataatcacc aaaattttgc cataatccga
    14701 gagataaaat cggtaatttc aaacctgaat gaccggattt acgatattcc attttttcat
    14761 aacgtgcact atctgcaata tacaattttt ctcctcctca tcaaatcttt ttaaaacgct
    14821 ttcttttttc tatttttagt ttactacatt ttaaagaaaa ggagttaatc aaaatgattt
    14881 tttcgtatat ttttttgcca tttgtttttt actcgttgat agatcagaaa ttgattacgt
    14941 gcttgctgga tttttttata acgtttcggt tgttttttgt aaaattgctg atgatagttt
    15001 tctgctggcc aaaacgtacc ggctggttcg atttttgtga cgattggttg gtggaaagtt
    15061 cctgaactgt ctagtttctg ttttgattgt tccgctaatt ctttttgtcg ttcgacttga
    15121 taaaaaatca ctggacgata ttgcttccct ctatcttgaa attgtccaaa tgtatctgtg
    15181 ggatcagtaa tttgccaata gattgtcagc aactcgctat aacttatttc tcttgtgtca
    15241 taaatgatct ccacagcttc cacatgacct gtgtaaccac cacttacttg atcataattt
    15301 ggtttttcaa catgtccgcc cgtgtatcct gacaaaacag ataggatccc ttttttcgat
    15361 tcaaacggct cgaccataca ccagaaacat ccccctgcaa atactgcacg ttcttgatag
    15421 tcagcatctt gcatggcatg cttggaaaag tcatattttt gctccttatc aaatccttcc
    15481 gtaatcaata aataaaagtc cattacatca ggcgtcagat tcattcggat agctaatgga
    15541 cgtagtgttg cttctagttt tgataactgt tctttaacgg atatattttc tcttatgtca
    15601 ttctttgtct gaatcaatac ttttcgttcc caatctcgtg tattcggatt caaaatcaga
    15661 ttataaagtg tttgaatgaa tatttgttcc tcatccatag attctcctcc tttccgaaga
    15721 agacaagcta gtgactttag ctgtaattta tcataaagtg aggaaataag cgattacttc
    15781 atccccctac agatttttcg tataaaaaag agagaatcct tatcgacaga ttctcctttt
    15841 taatcaattt attttataaa tgaaaagtta atttaccttt tatggaaaac tattttaaaa
    15901 aataaacata aatatgcagt taatgtaaca agataagaaa taatagtcgt tactgtataa
    15961 cttgtgctta ctttaatatc agcaactcct ttgactccgc ttatttgtac caatccatta
    16021 gcattcacct gctcctttac aggttgatga tttatttcta cattcgttcc taaataagca
    16081 tatacaggaa gtgtgattac ttggctcttc tcattagtta ctctatatat gatagaacta
    16141 ccagttgttt tataaggaag gtttacccat tgactgccaa tctttgcttc gtgttttatt
    16201 atatgctcct tattctccaa tgaagcgata ggagaataat catataaaaa ataatccatt
    16261 cctttagtgg tatacgcctc tactgtttct ttgtttacaa caatatgtcc cggctgagaa
    16321 attatttttt gattgaaatt ttttttacta gaaacagtta aagtgattaa taacaaaaca
    16381 gtcataaata aataaaatag ttctcttgtc ttattgtatc ttctattttt gaaaaatatc
    16441 atcgttaata tcagagaaga gaatattatt tgcaagctta agatccgcca aggaaattgt
    16501 aacaatttgg ctggcgtatt ttgcagaaga taccatggca ccaaactagt tgtagctagc
    16561 caagtcaaac acgtaccata ataaatcttt ttttctatgt ttgttaattt tttaaataaa
    16621 atcaggggaa tacataaaga taacagacca actagtccca ttgagtaagt agttaagtcg
    16681 ttgttcatag cttcagtcag actatcgaac acattagatg catgagcata taaatttgtc
    16741 acgctaggtt tgttgatttt ttggtagagc atttgttcaa acatcggata ccaaaaagca
    16801 ctcgtcagaa ccaatgtcat tcctgccgca ctaaataatt ttttaaatat agcaataaaa
    16861 tctgcattct tgaaaccttt agaaaccaaa cgaataaaaa taaaaatgat aattaatgct
    16921 gtactcatta atgttgatag tacatggctg tacactagca aactcatact caatgctagc
    16981 tttacagacg gatacttttc ttcttttcca gagatgattt gataagcata aaataataca
    17041 gggataacaa gtgttattgc gatagcttcg gcaattgcgg aacgatagta gatgtctact
    17101 gtccgataag cagagaaaat atagagacaa ctaaataaaa atgccgcttt tttttgtttt
    17161 aaaaaccgct cgccataatg ataagctacg agtgttgtac acaagttcaa taaccaaacg
    17221 taaataatat agcttacaat caaatttttt gtcatccaat ataacattac agctggaaaa
    17281 aaagtcagaa agggataaaa ataatttaca ccatatccag tataattgaa tgtctcataa
    17341 ttaatcggac tggtaaaaat agtcgataac cccttgattc gagataagtg aaaagaaaga
    17401 tctccagtgg tataaatact ctgtgtattt ttgaaaaaga tgaaagaata tatactacta
    17461 actataatta atccactcaa tattactact tttttattta ttttcattct tcttgtttat
    17521 ctccttttta gtcacatatc gagaatcttt caacttatta ggtgcaaatc gtttaaaact
    17581 tgaatacacc actaaacaat aagtcagaat actgatccat aaagccatga gagcagttgg
    17641 agtatatttt gttgtaattt ctatcttatt ttctccagca tttacatcca cattcactaa
    17701 gccatgtttt ttataagagt catttacgac tttaccgttt acttttacct gtgtacctaa
    17761 ataagcatat actggtactg tcactttttg ctttttgttg cttgccactg tgtaagtaat
    17821 gttattatct gcagctttat acttcccttt ttcccacgat tctcctactt ttacttcatg
    17881 tttttctaag tgaggccttt cttttatcgc ttctgttggg gcgtaatcgt ataatcctcc
    17941 catatctgat gttgtataac gtgggagtgt ttcttcattg ataacaatat gaccgtgttt
    18001 tgattggata acttttgcgt aattttcttt tgctgctata gacgtgacga gtagaaacac
    18061 cacagtagcg ccaatgatta accatgaata ttttttattc atagtctttt tcataaaaac
    18121 aagcgttatg atcattgaag aaaaaaagat ttggatacct aagatacgcc aaggaaactg
    18181 gagtaagctt atcggtgttt tttgaaacaa aaaccatgga aataaattag ttacgcatac
    18241 ccatgtagaa actacgccaa catagatgat tttttctttt ttctccattt ctttcagtat
    18301 gaataaagga aaaatcaacg caaccaatcc taataacccc atagtgtatg ttgtaatatc
    18361 gttatttaac gcttgaatga tactatctga aatagaaagt gcttctagct gcaaatctgt
    18421 tttgaaggga ctgtgtattt cttgataatt catctgctgc aacataggat accaaaaata
    18481 gctagttaat gcacaagtca aaaaaacagc ctttactaaa ttcacgacca caaattttat
    18541 atcgtttttt atttgcttgc ttaaaaacaa acgaactata atcatacaag caatcaatgc
    18601 actacacaat agtgtagtta agacatgagt atacacaagt agacttatac tgacagataa
    18661 agcgatgact tttttttctc tattgaatat caatccataa gcataatata aaacagctgg
    18721 gataatcgta agagcgattg cttctgcaat tgcggatcga taatagatat caactgttcg
    18781 ataagttgca aaagtatata aacaactaaa taaaaaagca gcatctatct ttttaaaaaa
    18841 cttttctcca taataaaaac taatcagaat cgtacaaata tttaataacc aaatatatag
    18901 aacataactt aatattaagt tatttgaaat tccataaaaa acgactgcag gaattattgt
    18961 aagaaaagga taaaaatagt tcagtccatc tccataattg ttaaatgttg tatagttgat
    19021 tggcccttca aaaatagaag ataacccttt gattcgtgaa agatgaaaag ataaatcacc
    19081 tgttgtataa atttcttgag catttctcat aaaatttact gaatataaag tactaagtaa
    19141 gataagaact actaaaagaa atgctttttt acttattttc atagatttgt tgttctcctt
    19201 ttttaattct attttcagta tactagaatt gtcttttttt aataagtttt gcgttaaatt
    19261 tttcatagca ctagtatgat atactagaaa agaatcaatt tttattactt ttaaatagga
    19321 gaaaaaaatg aaaaaaaaca aaaaccaaat aatagatatc atttttatgt tatttctaag
    19381 ttttgcttac gtcatccctc tattcatgtc taagggaatc tatcatagtg tcaatcagga
    19441 tacttacttt catttatctc gaatcatagg actagataat gtatggagtt ctccagtaaa
    19501 cttcaataac ttcgatcatc atggaacaat gatgaatatt ttttatccat ggcttactct
    19561 ttatccagct tttttatttt ataaaatgat gggaaatctt gttttaagtt ataatatcta
    19621 ctattttttt atcacttttt taacaatggt cgtttcttat ttttctatga aacaaataaa
    19681 aaacaatcgt tatatttcgc tgttattttc gattatttat acattttccg cctatcgtgc
    19741 cattgacata ttccatcgtg cttcattggg agaagctgtc gctttaacat ttttgccttt
    19801 gatactaatg gggtgttacg aaatttatat tagagattac caaaaatggt actggttgtc
    19861 tattggcatg acgctagtag tgtatactca tttactttct gtcgctatgg tttctgtatt
    19921 tattggagga acgttgtttc tttcttttta tttttgggac caaaaaatcg ctagactctt
    19981 aagtctttta aaagctaccg tcctcacctt ttttttaagt gcgggattct taattccttt
    20041 tattcaacaa tctagagcac aagaattgaa agtcccactg ggaaaagaat taagtggtat
    20101 ggctcctagt gatatgctga cacatatttt aaataataac tataacaact atactattgg
    20161 attattctta tttctcggat tgattggtgc ttttattttt ataaaaaaat taactgctga
    20221 tgatttattt attttctttc ttggtgtatt tgttcttttt tgttcaacca atttatttcc
    20281 ttggcaactt tttaaccata caccagttaa gagtctacaa tttgtctggc ggttaaatgg
    20341 tttttcttct ttatttatag cctatacgat gagtatagtg atctattata ttttttctac
    20401 taaaaataat tcatggaaaa taatggtctt tacgtttctt gcactaatat tgcatctctc
    20461 aggagtaagc aattctatcc atgctaacaa agattcatta acacttatag cacctgaaga
    20521 cgctgttgct atagctgaaa attacaatca tactgattat gcaaacaaag agtcaatcta
    20581 tcatcctgat atgattaata atgatcaata tttactaggt aaccaggaaa taaatccaac
    20641 aactcatttt atgagtaaca aacttacaat tgaattagat aattcgaata ataaaaatac
    20701 tgtcttgacg acacctattt atcgttataa agggcaagtg gcttctatta atggcaaact
    20761 agtacaaaca aaattatcta agtttggcac aactgaatta actattcctc caggtataaa
    20821 taaagtcgtt attacttatc agtacacaaa actagcaatt gcttcaagat atttatctat
    20881 cgttactttg attttatttt tactttatcg atttactttt tctaaacaaa aacaaccgag
    20941 aagagaagta caacattctc actagatttt tatttaagaa agacttttta gctgtacaca
    21001 taaacaggct aaaaagtctt tttttatatt tctttaattt gcttcataca ttatttaaca
    21061 tcgatattta tactgtgaaa catgcgtttc atccattatc aaagtatttt tatcgataat
    21121 aaacttcatc caaaaacaga ccttgtgcag ggacggtttc tcctgcatgt tgccgcactt
    21181 tgctttcaaa tatttcatcg attgtttcta tcggcatggc acctgtgccg atttctagta
    21241 atgtccccat gataatccgc accattttat ataagaatcc gtctccgaca aaagtaaaat
    21301 gaagcatttc tccctttcgt tcgatagata attcatcaat cgtacgcact gtggattttt
    21361 ttgatttttt caatgcagaa aaaccaatga aatcatgtgt acccactaat ttttcgcaag
    21421 ctgcattcag cttgtccata tccaactctt gagggaaata aaagctgtgg tttcgctcaa
    21481 atgctgaagg aatcacgttg ttccaaacat aatagctgta ttttttgccc accgcattat
    21541 atcttgaatg gaagcgctca ggcatttctt cgattctttt gatgacaata tcccgcggaa
    21601 ggtaacgatt cataaagtcg atcatttctg ccagtccaat ttctgtgttt gttttaaaat
    21661 ttgctacttg tcctcgcgca tgggtgccag catctgttct tcctgatcca ataatttcta
    21721 tctcttcatt tgtcatctga tgcaatactt tttcgatttt tccttgaatg gttttatcgt
    21781 catctccaag tctttgccat cctgaatatc tttttccgtc atattcgatt gtcaacttga
    21841 tgtttctcat attttagtgg ctcctctgtc ttccatttgg tcttctctct acctttttaa
    21901 gattgtagca caaaatatag ctccttgctt ctctaagaca gaagctatac aagaaaactg
    21961 tggaaatata tatttcagaa aaacaaaaaa cagccaccgg atgaagtatc cgctgactgt
    22021 tcaatttgac ggctcaagtt ttactgtttg agatgcaaga tactcaacca ggcaatgggc
    22081 acataattaa agaaaaaact gattctttgg caaaaaccta ttttattcag taatggccaa
    22141 gaatttatag aaggaattct tgccagtcca taaatcgccg caaaaagcaa actgatcaca
    22201 gacagtccaa ggtataagtg gcttacgtcc atttgcccat tttttcgata gatcaagaaa
    22261 agaagaactg gaaacaataa aaaaccactg taacctaaac ctgatccgat attatggacc
    22321 catgtcgaga cattccacga agattcatgg gtattgatac tgaacaaacc tgtaaagata
    22381 caatcaccta taccatagaa cgcgatggct gctgtaacta gggtagctaa cacacgagat
    22441 gtactaacaa atgcagcata caatgcaggc aatgacagga tgaaaaagat ccctgaaacc
    22501 acagaccaaa taagaaacgc ttttcttaca ggactggaaa cctctccaaa tacactgatc
    22561 acgctcttca tttgatctaa ttcaggataa tacaatccta atacgtacgg agtcaaaaaa
    22621 tcactcagca cgcctaataa taaaaagaaa aaaccaaatt tacgtaaaaa atccatgata
    22681 cgcctccaat aacaattacc tatgctttta attgttatta taaacgactt tatcttgatt
    22741 taacagaaac ctctatttac acgaacatct atataactca ttaaaaactc gaatcatttt
    22801 aaaaatacta aatatctaac gttcaacgtt cacttaatcc atctaaacaa tctttcaatt
    22861 tgattttttc cccacttctt ttacgcgcgc ctagctctcc tattgcagta aacaacagat
    22921 cacttgacga atttaaggca gtttccattg aatcctggat aacaccgata ataaatccaa
    22981 taccgacgat ttgcatcgcg acatcattag agataccaaa caaactacat gctaatggaa
    23041 tcaataacag tgaaccgccc gcaacacctg aagctccaca agcagctaaa gcagacaaaa
    23101 tacataaaag gactttcaaa acaaaaggtg cttcaatccc taaagaatgt acagcagtca
    23161 acgtcatcaa ggaaatggta atcgcggcac cgcccatatt gatcgtagca cctaacggga
    23221 tcgatatgga ataagactct tttgtcaatt tcaatctttc agccaacatc atgtttatcg
    23281 ggatatttgc ggcagaactt cgggtaaaga aagcgggaag tgcgctttct ttcaaacaaa
    23341 agaaaatcaa tggataggga ttttctttaa taaacaaaaa cgtgaggaat gggtacacaa
    23401 ctaatgcgac aaacagcatc gtaccgatca aaacaagtaa aagctgcaga tactctgcca
    23461 atccagcgat tccagtcgta gcaattgatt gataaaccaa tcctaaaatc cctatcggag
    23521 caaatgcaat aatcacttgt acgacttgtg acaacgctat agatatctga tccaccaact
    23581 cttttgtcga ttctgaacgt gctcgaaaag ctagtcccaa caatacagcc cagaataaga
    23641 cgccaaggta attcgcatca ataagagatt gaacaggatt ttgtgtgaca ttcgtcaaca
    23701 tagtagataa aacactttcc aaactttgcg gtgctttttc agatactgtt tcttgtaaag
    23761 taataggaac agtaaaaatt gaacttacaa taactgctac aagtgcagaa agcaatgttc
    23821 ccactaaata taagatcaaa ataggcttca cgaatgtctt attcccaatt ttatgcttgg
    23881 caattgacgc catcgtcaag aaaaaaacca gaataggtgc gattgccttc aagcaactga
    23941 caaacaacgt tcccaatatt gaaataaatg tccatgtggg gaaaaagata ccaaacaata
    24001 ttccgactaa aataccaaca acaatacgtt gaactaacga catatccaaa aacttctcta
    24061 acaaatgacc accctttcac aattttaaca atcattgtat cattttatgc aaagaaacta
    24121 aaggtataat tttctaaaaa ttctaacaat acaaacaacc atttcttgct aattttgtac
    24181 tttttctgtc tacacgttgt tttttgagat attctaagtt aggatgttat taatgggcaa
    24241 cttatcagga aacgttttct tatgtttttg tccaattttt ttgtgaggtg tttattcttg
    24301 aaaaaaataa agaaggagag ttatggcttc ctgacaatct ttgtcgcttg tataatgatg
    24361 gttttacagc gtatattgat ttcagcagtc actattccta catatgcaac aaaccctggt
    24421 cgattctaca tctatacatt tttacaaact tccgttgtta ttggtgcaaa tttgatccca
    24481 ctttacatgg gttaccaata tcaaaaaatc gcccgtttaa aagttttaca ttatttaagt
    24541 cgttttgcct ttatattcct aatagccaca ctagtttgca atatcttttt ttacgtacaa
    24601 gctggcagct tgaatattcg tgattattgg cttatcctaa cacctatcag tcagaattac
    24661 tttacttatg ctgtttcttg tactttgctt ttttgtagta ttccttatac agttggattt
    24721 ttaaataaat tatcgaaaac aacaattaaa tatctactat tatttttaac aattggatta
    24781 gttggctgct ctactctatt taataaagac ccgtttgcat tacagaatgg acagagtatt
    24841 ttatggattt tttatttatt tttaatgggt tatggtttga aagaatggaa ctggaaagaa
    24901 aaggtccgct ataagcttcc tcaagtattt atcagcttga ttgttttatt cggtttgatt
    24961 attttgatga cacaaatttc actcatcatc agaggaaata cagatacagc gctgaggttt
    25021 tctaaaccat attctctctt cgcaatgtat tatactttct cttcgttttt atttttagaa
    25081 atgctatctg ataaattagg tataaaagga agaggaaatt ttcttgctgt atttttgatt
    25141 gtcactcaag ttatatgtaa tttcccttta gtttcttatc gcgtatctac atttatgaaa
    25201 cgtgaatttc caaactcagg gaaggcatgg ctgataaata tcgggcagtt ttttatttga
    25261 tatttattag cagtgtccct cctagttatc tttttatttt atatacaaaa aatccgtttc
    25321 attcatcgtt tcatcgaata cttcgctttt gattcgattg aacaattaac tcagcgttgg
    25381 aaaaaaagaa ttcattggct ttttgttcat aaaagttatt ttttagttgc agcatttttt
    25441 tactgcttta ccttcgtgca gattttcgtt ttagaaccaa aagaaggttg gaaagaaact
    25501 atccaggttg ccttaaaaat atttactcag cgacaagcac ccatgatcct gacaatcatc
    25561 attattatgg gatttttctt cttactattg ttgatttcca atcgtttttg gtacgccttg
    25621 actgctacct tgatcattaa cttactgctc actatttcga cagtaatcaa aatggaaatg
    25681 cgggaagagc ctgtctttcc ttcagattta aaaatgctga ccggattgtc agaactatta
    25741 tcgatggtta gcccagtctt attaatcgtg ggcggattga tccttctcct attattgatt
    25801 actagtatta ttgtacaacg tagactacaa aaacagtatt ccttgaagat tcattggaaa
    25861 cgtcgattta tcagtatcgc tgtattatta ggatgttttt cgggtgtctt tttcattaat
    25921 cacaaaaatt cgccttcttt cttgctgttt aatttattta aagtaaacaa gactttcttt
    25981 aaccagcaag gcgccgtaaa agataacgga ccagtcattc aattcctaaa caatatcgac
    26041 attaaaatca tggaagaacc tgctggctat tcgaaagaaa aaattaatgg aataatgaaa
    26101 aagtacaaca caaaggcaga tgaaatcaac caaacaagaa gtgactggtt aaacaatcag
    26161 acgatcgttc tcaatttaag tgaaagcttc agtgatcctg cacgtgtccc taatctgaca
    26221 gtacctacag accctattcc ttacatcaaa agcattataa cagaaggaac aggaggattg
    26281 atgttatccg tcggttatgg tggcggaaca gctaacatgg aatgggaagg actgacaggc
    26341 ttgagtatca gtaacctgtc tgcttcgtta gttacacctt atacccagct agtggagcgt
    26401 cagaaaataa gtccaaatat taccgatcta ttcgatgaaa aaatagccat ccatcctttt
    26461 acagctgctc tttatcgcag aaaacaagtg tttgataaat ttggttttga caggttctat
    26521 tatttagaca gtccggataa attaacttat acggataaaa tagaacaaag cccacgcata
    26581 tcggatgatt cagcatacaa agaaaccttg aaagctttac aaagcaatac ggaaacgtca
    26641 caattcatcc aattatccac tatgcaaaac catatgcctt atgatgatta ctatagtgaa
    26701 ttgaactaca ctgctgaagg aactgctgtc attcctaata gaagaaaaga attacaaact
    26761 tatatgcaag gccttcacta taccgataca gcagtaaaaa aattcatcca agaaatcgac
    26821 aaaatcgata aaccgattac ctttgttttt tatggtgacc atttacccgc aatctatagt
    26881 gggaatgatt cgaaaaaata tggattagaa cagcaccaga cagattattt catttataac
    26941 aacaagtata gtcgtgaaca agcagctacg ttaaataaag aagttgttgc tcctaataac
    27001 tttggtgcta tggctcttga gcatgcgaat atcaaagtaa ctccttacta tgcgttgttg
    27061 acagaagtat ctaatcatct acctgcatta acaattgatc ctacagaaag cttgtctaat
    27121 cgttttaatg gaaaacaggt ctttgttaca gaggatagtc aaattatcca agaaaaaaat
    27181 ttaactgaga aacaaaaaga aattctagaa gattatcgtc tgatacaata tgatcttgtt
    27241 gcaggtaacc agtacagtgc aacatgggcg acacaaaaaa taaaagacta acaaataaac
    27301 tgagcaaggg gttgtgataa aagacatgtc acaacccctt gtttatctca ttaatttgct
    27361 tgtccataaa cgatttgatt aaagccattt ttcaattcat tattcgctgt ctcgttgaag
    27421 taaagataat tatcgggatt cccctgattt tgttgtgtca ccaatacttt tggctgattt
    27481 tgctgaaacc cgaatagata gacgtgcttt ttcagatatg gttctgtctc tgcatcggaa
    27541 tagacggctg tgatttgata gtctgcttga cctgtcccac tttctgacca ttctgcagaa
    27601 acaggcactc caccaatagc catcttccac tccccgttca aaatagcttg agggacttta
    27661 agaccatata agtccacact catttgattc gtataacttt tgtattgttg tcccattgtt
    27721 tggccccatt tggtcataaa tgcctctaat tgtgcggatt tgtctgcatt ccacagtgtt
    27781 tgttcaactt gttcgctgct ttctgaagag gaagatgaag cctctacaga agaacttgaa
    27841 gtagatgttt gagttgtttc agtcgttttg gaagactctg acgaagtaga ggtatctgct
    27901 gtttctgtac ttgctgcagt tgacttgctc tctgtgctag ttgttgtagt agttgcaact
    27961 gttgctttcg ctgtttcttt tgttgattct tttttcgatt cattcccgtt ggtacaacca
    28021 gctaacaaaa aagctagacc aattgttact aaaatccttt ttttcaatta aaaaactcct
    28081 ttcgattact cccacttctt tattctatca aataagcaat gtgattttat tgatttttcg
    28141 tattttctca tcaatctttc aaatcttcta aaaactgaga agcacccgct gatgttgtct
    28201 gtaatacatc agcgggtgct tcttttattt taatcttcgt gattttccaa tttacctcac
    28261 gctttatatt tgtatttgaa gaaacataga cttttagtca tcatcgtctt cctcatccaa
    28321 atcttgtcgg acagtttttc cttggagtct ttgtacttgg tagatgattt ctttattgaa
    28381 acttaaaagt tcaacaagca attgttgcaa gccatatttt tcttcttttt cagctaattt
    28441 gattgcccgt gtgataaaga cattttggaa atcaaaatct tttgcaaagt tttccaacat
    28501 tgttgaggct ggtaaatatt tgttttgacc atgttcgtaa atttttccat aacgcatgac
    28561 ttcttctaaa gtagacggaa cgatttcatt ttcaatcact aactcatgag agattttatt
    28621 gaaccatctc gcttcttgat cagcatattc tgaaaaaagg gtacttaatt cttgcgaaga
    28681 tgtacccaaa acatacaatt gtgcttggat caatttcatt tcttctaatt taaaatttgc
    28741 taatacatgc cccaccatgg cgccagcagt tggtttatga tggtcgatat ctgcttgttt
    28801 tgtttcttga acaaattttt cttccactat attcattgat ttacgcctct tttacttttg
    28861 ctgattggac ttcataacct aatttttcca caactgcagc taattgttca gggtggactt
    28921 catctggatc aaagtttgct ttgattttgc tcgcattaaa caaaactttc gtattgctta
    28981 ccccaggctg ttgagaaacc gcatgttcga ttttttgcat acagcttgga caagctagtg
    29041 tatctaattg cataataatt tttttcatcg tagttcttcc ttcctttttc ttgatagtct
    29101 tattctagat tacagcttcc ataaaaaaat tgattcttat caagttttac gatttttctt
    29161 ctttgctttc gtgtccaaaa tacaataaac gcatcgcgtt aaagataacg atcaagatac
    29221 tagcttcgtg aacgaacatc ccactagcca tgtaaataaa cccggcaaat aagccgttga
    29281 gtaaaaataa gacgacacca atggcaatca ctacattttc acgggtattt aaaacagttt
    29341 tcttagccaa gcggtaggca tgaaccaacg attcaaaatt agattgcatc aagacaacgt
    29401 cactcgtttc gatagcaaca tccgttcctg atcccatagc aatcccgata tcagccgcag
    29461 ccaaagaggg agaatcattg attccatctc caataaaagc aacgcgcaat ccttgttctt
    29521 ggtatttttt cacaaattga accttttcgt ctggtaataa ttcagcatgg acttcatcaa
    29581 tgcctaacat atcagctaca taatcagcag tcatctgatt atcacctgtc agcatcacta
    29641 actgttttgc tcctgcttgt ttcaacttag ctaattgttc agcgacttct ggtcgaatca
    29701 catctgacac acctatgatt tgagtgattt ttttgtcgat tgctacaata atgaccgagc
    29761 tgcctgtttg ttgtaattga tgtaaatctt gtacttgctt tggtgtgaga gaaagtttgt
    29821 gagcggcaat caatttggtg ttgcccgcac aaactgtatg accatcaatc tcagctgtga
    29881 ttccttgtcc cttgattgtc tgattatctt tgactgctaa ttgttgaaaa tcaatgtctt
    29941 tctcctgagc ataatggatc acggcgcgcc ccagaggatg atcggataac gtttctaatc
    30001 gagccactaa agctaagctg tccccttcat cctctgtata gttcttgata gtggatacag
    30061 cagtagttcc tttggtcaat gtaccagttt tatcaaagac aaaagtatcg attttggaaa
    30121 aagtatccat tgcttcaccg cctttgacta agatgccccg tttcgctcca ttaccgattc
    30181 ctgctacatt cgaaacaggc gcaccaatga ctaaagcgcc aggacaacca agaaccaaaa
    30241 cagtgattgc taaacgaaaa tcacgactaa tcagaccaac aaccaacgca ataagtaaaa
    30301 cggttggagt ataatactga gaaaatcgat caatgaattt ttccgcatgg gatttggtat
    30361 cttgtgcttc ttctactaat tcgataattt tggcaaaggt cgtatcatct ccgaccttag
    30421 ttgcttcaat cgtcacgtag ccatctgcta aaatcgttcc agagaagact tcatctccac
    30481 tgattttgac gacttgtcgt gattcaccag tcactgcggc ttcatccgca taccctttcc
    30541 cgtcaatgac tcgtccgtcc acaggaatag ctccaccagt ttttactaag acgatatcgc
    30601 cttcttccac gtcatcgatt tccacttctt cagtcgttcc atcttcttgg acaacactag
    30661 cagtagatgg ggccatctcg gtcaaagcct tgattgactc ccgcgttttg gtcaatgttt
    30721 tttgttccag atagctacca aacaaaaaca agaatgtaac gatagctgac tccttgtatt
    30781 ctccgatcat aaaggcacca agcacagcaa tagtaacgag taaatcaatt gagataactt
    30841 tgactttcac tgcctgatac gcatggatca taataggtat gaccgcaata atagaagcta
    30901 tggctagagc aatgttatag aacaagacaa aaccagtaaa ttttccgata aaaccaaaaa
    30961 taatcaacac agctgataag gcggtgatat gatttcgatg ctcttggatc cacttttgta
    31021 atttcatctt tctccctctt ttcctttcat tcttacgaga ctaagtatag atacttatcc
    31081 atcataaaaa attgaccact atcaagttta gtcaaaaagg cggttttctt ttggtagaat
    31141 acactacaaa aaatagttag caagaagact cactaaaagt gatcttgcca actattgtcg
    31201 tttctatcga aattttcatt gaatgatttt cctgtacagc atacatcgga gatctatttc
    31261 tttatctaag atttttatct tgctaatttt ttcctttagt ctttcaaatc cacgtcgttc
    31321 atagaattga tacgtacagc cactatctgt aaaaaggaat atttcttttc ctttctctct
    31381 tttttcaaat tctgttagca acttggtacc gatgcctttt gttttcaaat caggatttgc
    31441 agctaaaaaa atgatctctc catctggcga atgcttcttt aaatatgctg caagcatttg
    31501 ttcatttgct gtctcgtaag ctcctttact ttctttagca aatatatttt gtaaaaaatc
    31561 gaatatctta acatatattt gctgtccaaa agaacgatgt ttcttttttt ctccttttat
    31621 ctccgccagc aaaacacctg ccaactcgtc ctctgcatac aaagcaagaa tttgagttgc
    31681 tcgattcaac tccatataca aaaaatatct tccgtaaaga ttcagcagaa gttcattttc
    31741 catatatctc tcaaagttca ttccttcaac agcaaaatag atagcttttt tgtaatcttt
    31801 cttttctaag ggtttaaaag ttagtttcat catttcgcct cctcaacttc aatgattgtg
    31861 ttatacttac attataatag acacagtgtc tactatctat aatcaatcat ctataagttt
    31921 gtcgattaag cgacagcttt gctttgatat gtcggataag gagataggta aatgaaaaag
    31981 acagatagac gcgtcaaaaa aacagaaaaa gctttggctg aaacactatc cacattatta
    32041 gtaaataaaa agattcaagc aatcactata cgcgaactaa cagaaacggc agatgtacat
    32101 cgttcgactt tttatacaca ttataaggat atctatgatc tatatgatca attagaaagc
    32161 aacttttttc gggatctaaa tgaaattctc tcttatgatc cagcccatag ctatgaggaa
    32221 ctatacactc gcttgattga ctatgtttac gcaaatactt ttttatatcg cttatttatc
    32281 ctcaaccatg gtgatatttc gtttcagaag aaagttaaac aagttattga agaaaactat
    32341 ctgaacatat ggctatatga agacaagaaa acgattatca cagaagaaat gagattcttt
    32401 actacgtacc acgtccaagg ttgtctatcc gttctcaatc ggtgggtaga aagcaattac
    32461 aagtactcca aaaaagaatt attagaactc ttacagaaac tgaatgatca catagagaaa
    32521 atcatgcctt gaataaaaac atactaagct tagttaagaa gaaccgtaac cttctcgtat
    32581 aacaactgaa tcttatttag ccaaattgtt tactgaagat tttttgagta aaatttctat
    32641 aattcctact tattgaaaag tagtttgtag tgctgcttct ccctctctaa gtgatatatc
    32701 ttgttgtacc aatcaaattc acctcatcat taataaatta cttttaggct aatttattac
    32761 aacttatagg ttgaagctgt aaccaattta tagtcatatt atatttttat catccagttt
    32821 attgaattct tctcgtttac ctcaattcat tttcaagggg aatcagctca tcatctaaca
    32881 ttttacgcca tgctcgcttc atttcttgtg ggtatttaaa tggtttcgct tctggatgtt
    32941 tggctttata tccttcgtaa tccttactgt tgataattgc tttcatatta ggaattggat
    33001 cttcacctgg catatattgc agattagaag catgtaattt tttctcagaa acaaaccact
    33061 cattggaaaa gacatgaatc aaatcatcac ttacttgtgt aaaatagcga cgtttaatga
    33121 caaagatatc ttcattgcta tctagtgtac cttcatttct ttgtgcttaa attcaaagat
    33181 aattttctca ataaagattt ttgattccag taagattcct ttactccttt tacaacatag
    33241 cgttgtcctt tgttttgaaa catcttcttc ccctctttgc cattaaaaat agcccaattc
    33301 tctataaaga agattaagct tcattattat aatataaagt ttaaatttgg tttattactt
    33361 actttttctt attctctagt aagctaatat tgaaaaaaat gtaatcagat cattattttt
    33421 atatgttgta acttcctgat aagaaaacta attaaaagaa agaggtaact acataaacta
    33481 attaaaaaag ctaacttcat agttggagga taaatggaag aaacttcaat gattgtttct
    33541 ccttgtgttc cttcaatcga cactagtcct attatcaatc tctctttttt gtccatttac
    33601 ttttgcgact gttccccaat aatcaaatac aggaagcgtt attttttgtc ctttagattc
    33661 atgtactgta tagactattt tcgaatccat cacttgataa ggcatagtat gccattcctt
    33721 gccaattctt acttcatgat tctgcaaatg ttctttgtat tgtaatgctt ccgtgggtgc
    33781 atagtcataa agtcctccgg gaaggagtgt cgtgtaacga ataagatttt cttgattcat
    33841 taccaagtga tctctctgag tgacaacttt atgttcatag tttgcttttg ttgcagcaga
    33901 tacagccaac agtaataaaa caattgccgt tatattaact acttttcttt gcttagctga
    33961 tgtgctcttc atgaaaacga acgataaaat caacgagcca aacagtactt gaaagcctaa
    34021 gattcgccaa ggaaattgaa tcatttgaat gggtgtattt tgtaataaag accatggaaa
    34081 aaatttggtg gataagagcc aagagactac cgcactaagg taaatcactt tttctttgcg
    34141 attaaatcct ttaaaaaaga agagtggaca gatcaaagaa ataataccaa tgattcctac
    34201 cgtaaaggtt gtcaggtcat tattcatcgc accaatcaaa ctatccgaga catttaatgc
    34261 ttggtgttgt aaggtcgttt tatctggctc attaatcgat tgaaccatga tttgtcggaa
    34321 catcggatac caaaagtatg ctgttaaaag aatcgtcatt cctactgcct ttgacaactc
    34381 cacaatcaac cggatgcttt ctttttttct tgtttcactt actacaaagc cgcctaagac
    34441 tagtaagcca acggctatta cactcataaa agtggaaaga acatgtgtat aaatgagtaa
    34501 gctcatactg actgctaatg gaattgcaca tggctttttc tcataaatca gtgcgtaagc
    34561 ataatacata actatcggta taaccgtaag tgcaatcgct tcagcaatag cagaacgatg
    34621 ataaatatca atcgtacggt acccataaaa tgtatataaa cagctaaata taaaagccgc
    34681 atcgattctt tttaaaaatt ttagtccata atgaaacatc agcatgatag tacaaacatt
    34741 caatagccaa acatataaga tataactaac gattaaatta ttacttatcc aataaaaaat
    34801 gactgccgga aaaaaggtca agtagggata aaaataattc actccgctcc cataatgatt
    34861 aaacgtcgta aaattgattg gaccagaaaa aattgaagat aaccctttga ctctagcaaa
    34921 atggaaggca atatcccccg atgtagaaat agtatgaaca tttctaaaat aattgattga
    34981 ataaagtcca gcaataattg ctaggagaat ggacaatatt atttttttct ttacttgcat
    35041 tacttaatta tatactcctt tcatggattg ctttatctag tataacttaa attttaaata
    35101 aatcgttatt tatttttact ctcacttaaa aaagcactaa aattataaga gagggaggta
    35161 atggtctgaa caccaagaat tgttccaaca tcgaattgct taactaatga gttcaaagct
    35221 taattatgtg gctcttatat ttaccgagcg tggattgcga tctctttagc gaaacaaaca
    35281 tctttcataa aaaaaacagc catatccgct ttcttggata tgactattta ctactacttt
    35341 actatctttc attcttcttg tctcacttat gataaggcgc tgggtatcat gagtgaatga
    35401 tattgctgaa tacaaatagt ccgaccacat taagttgatc ggacttcata agcattttat
    35461 tgactaatac ttttttgctt aagaattgat tctaacatct tttgctaaag ttaagcatcc
    35521 tatagttcct gcattatctg ctaacttagg tgtgacaatg tattcttcga tatttggcgt
    35581 atcgacatag ccgttaatta gttctaaaaa acgtctttgt actttctttt tcagatgttc
    35641 ttgttgcatg acaccaccac caaaaattat aatatctggt gaaagcatca gggtcgtatt
    35701 acaggcacat tgtgcaatat aatccgcctc gatttcccag aacggatctt ctatagaaag
    35761 atcttgtccc ttggttcccg tacgcatctc gatagcgggt ccagatgcca acccttcgag
    35821 acaatcatga tgaaaaggac aagtaccaga gaaagtatct cctgaatgtc tcttaactaa
    35881 cgtatgcccc atttctggat ggctaaaccc ttcaacaaaa atcccatctt gtatagctcc
    35941 tccaccaatt ccagttccaa cagtgtaata aacaacactg gataaacctt ttccatgacc
    36001 tgaaacatac tccccgtaac aagcagcatt cacatcggtt gtccaggcaa ttggaatttc
    36061 gaaatgctta ttcaacgttc caacaaaatc aaaatttttc caggcagttt ttggagtaga
    36121 ggtgatatgt ccataggtct ttgattcacg atggatatca atcggtccaa atgaccctac
    36181 tccaatgctt tctaattgtt ctttatagtg attaaagaaa tcaattacca aactcattgt
    36241 ttcattcggt gtagttgtag gaaaactcac tctttctaaa acctttaaat catcattgcc
    36301 aatggcacaa acaaatttag tcccacctgc ttctattgag ccatacattt cttactccac
    36361 cgtcctttta tctaggaatt cgttcttccc tgtctaatag tttttcgaaa aactcatgtg
    36421 gttccttttg gtaccaatag gcaacagaag aaatatcgtc ttgacgctca taaagcttga
    36481 tgtcatcatt tcctagggtt tggaaagtaa cacgtaaatt ttctttgaaa ctaatggggt
    36541 cgggtaaatg ccagcggtac aacccatgca tcggtaaagc atcatcgcca aaaccatgaa
    36601 ctggatttgg attaggtttt ccagcactaa aataatcccg ggtgtgatct cttttagatt
    36661 gaaaaggata gcccataaat agcgtattaa aattttgagc atatgccctt tctccaaagt
    36721 tctttttatg gaaagcccaa gcgccgccaa aataatcttc tgctcctgta gatgttattg
    36781 tagggaaatc ttcatctcca tctatataaa acttaaattc tccttccccc caccagtatc
    36841 tttccaatgc gcacaaagca aagtaggttc ctacataata cccagtgcct ttgattccat
    36901 caataagggt gtaatctttt ccttttgtcg tgatgggttc acggttccat ttagcatgaa
    36961 aatacatcgc atcatccggt acttcagtca atgcataatt aaatgtataa aaaatgtgct
    37021 taataaactc aggatgttca ttcgttaaag taatcttagc tttttttcga aatggcatct
    37081 caaaatagca attcatccct gatgttggat tcactacgat tggtaaggaa ttcacttcgc
    37141 atcttgtccc aaacccatta caaaaaaaat ctcctaatgg actttctaca gaaggagtgg
    37201 attcatcatc ccaataaata cgcaaaatta aatcacgtaa gacgaaactc ccagcgattg
    37261 ttttgtctgt cactgtcatc caaaaatgac gtaaaattcc agggccttcc atttctgcta
    37321 aagtaattgt ctctccagca tttatcggaa cagaaggtga cccttttctc ccaattccta
    37381 gatgactcgc tgtcattgcc gctttccctt tttcaccatt tttattttct ggtgaaatag
    37441 ttctactttc ctctcccttg tggagccata tattatttaa acaattcatt ttattcgtcc
    37501 tctatccttt cttcactttt ttgattattc atatagatga tttgttgttg tgcctccgca
    37561 ataattgctc caatatctaa attatcattt cctaactgca aagcgattga cattgccaag
    37621 ggaagattca ttcctgcaat tacgtgcact ctgtcagaaa tataggtaaa aaacttttga
    37681 tttacacttc ctccagccat gtcagttaaa acaattattt cgtctgtagt atccactaac
    37741 tctttaattt gttcatctat atcatcaatt tcttcgacat atgcacataa gctactaatt
    37801 tgattatgca tacctgttaa aaactctagt gtctctttaa aaccctcagc tagtattgaa
    37861 tggctagcaa tgatcaatct tctcataatt caaatccttt tcctttacgc taatattcca
    37921 aatgcagcca taaccatcgc aaaaacaata acgagtagaa tcaaaatggt catactgatt
    37981 tttttcttac tgagtagata atatgctgca gcagtaataa ttactggaat taatgttggt
    38041 aaaattttgt ccaacattgg ttggattttc atactaactt caccaaaagt gaatttaagt
    38101 ggcgttgaaa cactgataac tgaagcaatt aatgatccaa ctactgttag ccccaatact
    38161 gaagcagctt cagttaaata attaatcttt tcaccgtatt cagtaacaat cttcattccc
    38221 tgcttataac caaattcaaa gcctctcatt cggaaaaaga agattaccat gattaaaatg
    38281 aaccataaaa agattcctac aggattattt tctaaagcca tataagccgc aattgatccc
    38341 atgatggttg ggatcatcgt catcaaaaga gagtccccaa taccagcaat tggacccatc
    38401 gtaccaactt taaagtcatt aatcgcatct tttgcttcta atccttgctt ttcctccatt
    38461 gcaacacaag cacctaaaat aatggctgcc agccaaggct gtgtattgta atactgaaag
    38521 tgattattta aagcttcgat gtattcatca tcatctttgt ataattttct taaaataggt
    38581 gacaatgcgt aagtcacaga tgcaccttgc tgtgtttgat aattaaaggt acaaactgac
    38641 atcaaccaac gatagttcgc tttcctaaga tcctttttcg ttacttgata tttttcgctc
    38701 attttattca tcctccccaa taaacccaac atcagaagct cccgcagaag cagtgatttg
    38761 tgcatctttt tcagctctat gatacacatt aaatgctaaa gcgaatccta tgatcgccgc
    38821 tccaataata ggtaccttca aaaaagcggc taagaaaaat ccaacaagta aataactaat
    38881 gtatcgacct gtaggcataa agcgtaacaa catcgcaata ccaacagcag gcaacatttt
    38941 tccggctaat gttagcccat ccataaacca attaggtaaa acatccaaaa cgtaattaac
    39001 agcagactgt cctaaagtaa cggcaataaa tactggaatt gctgcagata aaccaaagaa
    39061 aattggcccc aaccacaaaa cactattcat tttcttaaat tctcgtttat tcgcatattc
    39121 ctgtgatttg cgaacaacaa aaccatttaa gattttgaca attacatcta gttgcacccc
    39181 taacatacct acaggtagtc ctattgctaa tccggcttct actccaagct ggctaccaaa
    39241 tgccgttgca ataattgcgc acgttccata gtccggtaca gatgagccac caaaaccggc
    39301 aactcctaat gacatcaatt gaactgtacc gccaactatc aaacctgttt ttatatcacc
    39361 catcaaaatt cctgtgaata atccccaaaa aactgcgtaa tttacaaaga tcatcggaat
    39421 cccataataa tcaacatgtt taataaatgc taatatagtc agcataataa tttgaaaaat
    39481 agtaaaatgc attatcctta ctcctttatc ctaattttga ttgaatttcc tgtgccttat
    39541 ccgccggaac aaattgtgta acaatttcta caccagaatc caacaatttt ttatacgtgc
    39601 tgatattttc atctgatgca taggctctag aagatagctg taatgcatta tctttaacaa
    39661 cagtaccgcc aacaattagt cggtcaattc taataccgtt atctactaaa tgaaggattg
    39721 tttccggctc tttcacgatg acaaatactt tttcctgatc gtatttcccc tttttaaaat
    39781 tcgcaatcgc tgtttcttca gtgataattg atacagccat tccagcaggt ctagctaaac
    39841 gcatacttga tttttggatg tcgttatttg cgacagaatc atcgatcacc atcactctgt
    39901 ttgctttaga cattggtgcc cattgcgtga caataatccc atgcaataaa cggttatcaa
    39961 ctctcgacat aacaatactc ataaatactt cctcctttat ctttcatttt cttacaagca
    40021 attaccatgc caaaacaaaa agaacttgat aaatcaagct ctttttacat aatcataaac
    40081 gtatttaacc tcatgaatcg ggatgtcgac actataataa tgttcaacga cactgaaaga
    40141 ttgcttaaaa tgtgtctgaa atttttgaaa ttcctcactc gtttcattga aataaatttc
    40201 atcttccaaa gtattttgtt ttactaatcg ttcaattaag caactaatgt gaacatataa
    40261 tccgaaacac gtattattat caaatgaaat tcctagatca ttttgtaaga tgtatatagc
    40321 atctgaaacc cgttctaata atttagtcgg atttaatatg gtcaattgct ccattaaatt
    40381 cgttagacta aaattcttca gaatattatt gttcaaaact ttcagttcat tatcattaaa
    40441 gtgtctattc aattgattgg agaattttct tatattcatt cctaaaatca tatcttcaat
    40501 tgaaaagaat tctacatctt ctagatgtgg atttagtgtt ccaacaataa aaagaatatt
    40561 gttctcttta cgaagctccg ataaatagtt ttcattcact aaagagtaat agtcacacgt
    40621 ttctatgaca agatcagtat cccttggaaa gctatcttca agtatccttt ttaatttaaa
    40681 cgaagttcct aatccgcttg cacaagaaca gacaatcata tccttcttaa tatttgatga
    40741 ttcataaata taatccacat tttgattttc ttttacttgt tcaagaatct gtttaagcgg
    40801 taaatctaat aatatctgat ttgcaacatc aatagccatt cttgttgaaa cattgtttag
    40861 tagtccgaat tttttttgag cgtctagttc caaatttaga taaattttct ccagtgaacc
    40921 catatcaaca agtaaaataa tttcttcata tccggtacac tcttgtaagt atctattaat
    40981 tttgtctgca atctcactac ttgaagtcat tagtggcata tcaatggcgt cgtatatata
    41041 tctctcaacg atcctattag cagtacttgc tatacaggtt gccgttagat ctccatgagc
    41101 caaaatcagt gctaaacgtt tttgctgaaa ttctttacta cacattaact taaataaaat
    41161 aaccaaaaca tttttttgaa tttttgttaa ataaatatcc cctatttgaa taaaacgatt
    41221 gactaattga acttctcgac tacaccaact attaatatca ttgatgaagg aatataattt
    41281 atcgtccgat gttaattgga tttgataaat ccaagaattc aaaaagatag acgttatttg
    41341 aagataataa tagtcaaatt ctatttgaaa atctctctct atttccttaa catactgtct
    41401 aaatcgaatc tcttgatagg tgggatatgt taaagctgaa ttattttgga tatcaacatt
    41461 cttttgtcta aaatatctat catatttctc aaaaagctgc tgcttatcag tgtctttctt
    41521 ccacgtttga taaagatact ctataagttc gttgatatta aaaacttcgt ctactttttc
    41581 caaatcatcc atttgaatat attttaattc attctgtctt ttcataacct tcatgccgtt
    41641 gaaattcacc cattgtggta aatgatacat ctgaatataa agttcttcca aatcatctaa
    41701 gaaataaaag gcattggcac aggtcaattg aatgatattt ttcaaattgc caacattttc
    41761 actaaagcga tagtttaata aaaaatctaa taattgttga gatatcagta ttttctttgc
    41821 tagtctttct tcttctgatt taaaaaatat cttgattaac tgttttcttt cataaagaga
    41881 acgactgtct aatgttggaa gtgttagctt tacaggtatt cttctcaaca aagttgataa
    41941 tagtttatcc tcaggtggtt ctgatgttgc aaatataatc cagcaacttg atgtgtacca
    42001 gttttcatta tcacccatcc gatgaaattt cctcgtatcc ataaactgaa aaagtttctc
    42061 ctgacattca ttgctcaatg catgaatttc atctaaaaat aagactccat tattagctaa
    42121 ttctgctaaa ccctttcttc catcttcggc tcctgtatat gcacctttta catgcccaaa
    42181 aaaattggac aagaaaagtt ctggattatt ggcatattca ctacaattaa ctgaaatgaa
    42241 agatgccttc tcttctatat aattattggc caacaaatcc ttatacacat aatttgctaa
    42301 atagcttttt ccagttccag tctcgccatg aagtaaaatg ggtaagccat ttggcggata
    42361 gtagattgct gctttaattt gagcaattac atttcgcaga cttccttgac ttccgataac
    42421 cctttctatt ccaaccctaa aagagctatc ataccatttt tccgcttcct ctgaagagct
    42481 aaaaatttgt tgatttggaa ctttctccgg taaagaaaag aagattactg gacgagtatt
    42541 gatttttcca aattctccat cttttacgaa ttcattcaaa tattgagaga caatattacg
    42601 actaacattt aatttttcag atattttctg tgctgtattg ttcccccatt tatttttaat
    42661 ctcagagttc tctatgacat aattgacaat gcgttctttt aattcttcca tcacagcacc
    42721 tcctcgacac tattcgaatt ttgccgacag tatttttcca taaatatgac ttgctaaaaa
    42781 ggctttgttt agatcattca attggaacgt tgcttgtttt gcttgatccg ctaagacgtt
    42841 gaaaagatac tctggcgaaa tcgcataccc caaaatatca acgattgttg caatcagatc
    42901 ttcttttgat tcttcatctg aaagtaattc acgatacaac atcgtttgtt tgcttactgt
    42961 gtcatattcc tctaaggatt catctgctaa aagaaccact tgttccaaca aacggtcgga
    43021 taaatattta tagaaaatca gccccaataa ataattttta tattctgacg catccatttt
    43081 actacggaga ttatctgccg cactaaataa cttctgattt aattctgcac ccaaaaagat
    43141 acactcctat ctcttattta aagttgctat ataccctctt attttagcat actcagtttt
    43201 agaaaaagca aaaaaataaa gaaacttatt cttgataaca agctacgtga cgtatatccc
    43261 taacacaaaa atagccatat cctttggttt ggatacggct atttgatatt actcattcaa
    43321 ttatcaaatc acagtaggtg tgaatctcat cgggatcggc aatatctact tctgattagg
    43381 atcaaaggtg attttctcaa taaaggtgtg atacaaagct ttaacttgct tcttctcact
    43441 ctggataaga aagcaatcca aactagtcaa tatctgttgg acatctttga cttgaatcgg
    43501 ttccccttgt tgttgaaaga tccctaagat ttcattctct cgaatttgta actgtcttcg
    43561 tttttcagtc aattcattta aacgatcttt taacatcgga aacaattcgg gactatcttc
    43621 caaagcagct tcctgttttt gctctaatag ttagattttg cgtaccattt tcttctttta
    43681 tttttcttcg attgctgatt ttaatatagt ctaaaggaaa tttatattta ttgtattctt
    43741 tataacttca agagaacttc gtttgagtat tactgtattt ttttaattcc ataagaaaat
    43801 tagttattac ctgccaccta atcagcaaat ctgaacctga gtcaatataa ttaatattat
    43861 tttccatctc caacattgta agtactgttg gtgttaatat agggactttg tacaattgta
    43921 tttttttgat tttatcatct gcacaaagga gattttttaa atctaatcct tcttttattt
    43981 caaaagttga attttttaaa tctataagag taaaaccaat tccttcatac tgaatttctg
    44041 aaaatcgaat ttttctccca gttgatagat caaggggcag aaaacctttt attacattgt
    44101 gagtgtcacc atcatttaca atgtcattag gaattttgat tttagaattc tgtggagcac
    44161 ctattggaag ataataattc attataattt tgtagtcttc atgaggaaat aaagtacttt
    44221 taggattgct aagatattct cgtaccgaat ctaagaatgg cttatccatt gaatcttcat
    44281 ccccatttaa actaaaaaac attcctacaa ttgcttttgc aactctaagt gaattcattt
    44341 tctttattga atgtgttaaa tcagacttac cattctttaa acttgtattg ttataaagga
    44401 aatttttatt gtcttctgca aatctaatat attcctcaac atactcacta ccaaaaaaac
    44461 tattacattt actacataac gtattggaat ggcaacagtt tttttgacaa attttataag
    44521 gtgcagaact tctttccgta tgctattccg attgtccttg acaatgagcc tcctcggatg
    44581 tgacgatctt cggcaggagc taacagttaa agttagactg cctcgctagc gttagcacat
    44641 ccgagctcat tatcaaggat tcgctgcgcc aat
//
LOCUS       DANJMI010000022        43587 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000022 DANJMI010000000
VERSION     DANJMI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.022"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            69..1151
                     /gene="uxuA"
                     /locus_tag="PWU81_001414"
     CDS             69..1151
                     /gene="uxuA"
                     /locus_tag="PWU81_001414"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL1110124.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(1259..2812)
                     /locus_tag="PWU81_001415"
                     /pseudo
     CDS             complement(1259..2812)
                     /locus_tag="PWU81_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2960..4101
                     /locus_tag="PWU81_001416"
                     /pseudo
     CDS             2960..4101
                     /locus_tag="PWU81_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(4227..5306)
                     /locus_tag="PWU81_001417"
     CDS             complement(4227..5306)
                     /locus_tag="PWU81_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL1110125.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(5409..5849)
                     /locus_tag="PWU81_001418"
     CDS             complement(5409..5849)
                     /locus_tag="PWU81_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL1110126.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(5906..6316)
                     /locus_tag="PWU81_001419"
     CDS             complement(5906..6316)
                     /locus_tag="PWU81_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL1110127.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(6496..6930)
                     /locus_tag="PWU81_001420"
     CDS             complement(6496..6930)
                     /locus_tag="PWU81_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1110128.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(7030..7878)
                     /locus_tag="PWU81_001421"
     CDS             complement(7030..7878)
                     /locus_tag="PWU81_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL1110129.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            8267..10033
                     /locus_tag="PWU81_001422"
     CDS             8267..10033
                     /locus_tag="PWU81_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDL1110130.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            10135..11394
                     /locus_tag="PWU81_001423"
     CDS             10135..11394
                     /locus_tag="PWU81_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL1110131.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            11541..12842
                     /locus_tag="PWU81_001424"
     CDS             11541..12842
                     /locus_tag="PWU81_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1110132.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            12845..13696
                     /locus_tag="PWU81_001425"
     CDS             12845..13696
                     /locus_tag="PWU81_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1110133.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            13709..14524
                     /locus_tag="PWU81_001426"
     CDS             13709..14524
                     /locus_tag="PWU81_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HDL1110134.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            14675..15841
                     /locus_tag="PWU81_001427"
     CDS             14675..15841
                     /locus_tag="PWU81_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL1110135.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            16071..16718
                     /locus_tag="PWU81_001428"
     CDS             16071..16718
                     /locus_tag="PWU81_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL1110136.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            16802..17746
                     /locus_tag="PWU81_001429"
     CDS             16802..17746
                     /locus_tag="PWU81_001429"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HDL1110137.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            17881..19332
                     /locus_tag="PWU81_001430"
     CDS             17881..19332
                     /locus_tag="PWU81_001430"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDL1110138.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            19353..20189
                     /locus_tag="PWU81_001431"
     CDS             19353..20189
                     /locus_tag="PWU81_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL1110139.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            20189..20848
                     /locus_tag="PWU81_001432"
     CDS             20189..20848
                     /locus_tag="PWU81_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL1110140.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            20974..21663
                     /locus_tag="PWU81_001433"
     CDS             20974..21663
                     /locus_tag="PWU81_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110141.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            21669..22625
                     /locus_tag="PWU81_001434"
     CDS             21669..22625
                     /locus_tag="PWU81_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDL1110142.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            22669..23181
                     /locus_tag="PWU81_001435"
     CDS             22669..23181
                     /locus_tag="PWU81_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1110143.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(23437..23985)
                     /locus_tag="PWU81_001436"
     CDS             complement(23437..23985)
                     /locus_tag="PWU81_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1110144.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(24074..25255)
                     /locus_tag="PWU81_001437"
     CDS             complement(24074..25255)
                     /locus_tag="PWU81_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL1110145.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(25491..26999)
                     /locus_tag="PWU81_001438"
     CDS             complement(25491..26999)
                     /locus_tag="PWU81_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDL1110146.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            27158..27349
                     /locus_tag="PWU81_001439"
     CDS             27158..27349
                     /locus_tag="PWU81_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110147.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            27552..28469
                     /locus_tag="PWU81_001440"
     CDS             27552..28469
                     /locus_tag="PWU81_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HDL1110148.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            28462..29535
                     /locus_tag="PWU81_001441"
     CDS             28462..29535
                     /locus_tag="PWU81_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110149.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            29683..30201
                     /locus_tag="PWU81_001442"
     CDS             29683..30201
                     /locus_tag="PWU81_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110150.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            30182..30574
                     /locus_tag="PWU81_001443"
     CDS             30182..30574
                     /locus_tag="PWU81_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDL1110151.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            30650..31585
                     /locus_tag="PWU81_001444"
     CDS             30650..31585
                     /locus_tag="PWU81_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL1110152.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            31718..32497
                     /locus_tag="PWU81_001445"
     CDS             31718..32497
                     /locus_tag="PWU81_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL1110153.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            32490..32807
                     /locus_tag="PWU81_001446"
     CDS             32490..32807
                     /locus_tag="PWU81_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL1110154.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            33051..34661
                     /locus_tag="PWU81_001447"
     CDS             33051..34661
                     /locus_tag="PWU81_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL1110155.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            34818..35666
                     /locus_tag="PWU81_001448"
     CDS             34818..35666
                     /locus_tag="PWU81_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL1110156.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            35897..36376
                     /locus_tag="PWU81_001449"
     CDS             35897..36376
                     /locus_tag="PWU81_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110157.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            36409..38637
                     /locus_tag="PWU81_001450"
     CDS             36409..38637
                     /locus_tag="PWU81_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1110158.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            38766..40409
                     /locus_tag="PWU81_001451"
     CDS             38766..40409
                     /locus_tag="PWU81_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL1110159.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(40482..41027)
                     /locus_tag="PWU81_001452"
     CDS             complement(40482..41027)
                     /locus_tag="PWU81_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDL1110160.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(41050..41535)
                     /locus_tag="PWU81_001453"
     CDS             complement(41050..41535)
                     /locus_tag="PWU81_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL1110161.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(41525..42337)
                     /locus_tag="PWU81_001454"
     CDS             complement(41525..42337)
                     /locus_tag="PWU81_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HDL1110162.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(42476..>43587)
                     /locus_tag="PWU81_001455"
     CDS             complement(42476..>43587)
                     /locus_tag="PWU81_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDL1110163.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        14474 a         7842 c         8500 g        12771 t
ORIGIN      
        1 gtagcaggac caaatgccgt acgaaaaaca ttacaggatt acttacaaga acatggagga
       61 aatttatcat ggaaatgact tttagatggt acggatcaca ggaagaaaag attaccttag
      121 atgaaatcag acagattcct gcggtcaaag ggattgtagg cacattgatg gatataccag
      181 taggagaggt atggcctagg gagaggatca aagaactaaa agaagaaatt gagtctcgtg
      241 ggttaacact aaaagtaatt gaaagcgtca atgtgcatga tgccattaaa attggattac
      301 cttcccgtga gcagtatatc gaaaactata agcagacaat ccgaaacttg gcagaatatg
      361 gaatagaagt catctgctat aattttatgc ctgtatttga ctgggtgaaa tctgaccttg
      421 actaccgctt agaagacggc tcatccacgt tagcttttat atctgatgat attccagatg
      481 atccaagaga aatcgtcgcg cgcatcgaac attccagcaa tgaatttgga ttgccgggat
      541 gggaacctga gcgcttagct cctatcaaat ccttgcttga agcctatgca tcagtagacg
      601 aagaaaaact tcgagaaaat tttgcctatt ttctccaatc aatcatccca acatgtgaag
      661 aggtaggggt caaaatggct gtacatccag atgatccgcc atactcgatt tttggattag
      721 ctcgaatcgt aaagaatcga gaagatttag attggttgtg tcgcatagta gacagccctt
      781 ataacggaat cactctttgt acagggagta ttgcagaaga tccagaaaat gatgtatatg
      841 ggattttagc agaattttgc caaagagatc gaatcccatt tgcacatgtt cgaaatatta
      901 aatttttagc taatcgagat ttctatgagg caccacataa atccgaatac ggttcattag
      961 acatgtaccg catactaaaa acaatgtatg acaacggatt taacggatac atccgtcctg
     1021 atcatggaag aatgatctgg aatgagacag gaagaccagg atacggctta tatgatcgag
     1081 cattaggtgc tgtttatcta aatggattat gggaagcaat cgaaaaagga aacaaaagta
     1141 aagataatta gtgaaaaaaa ctgtaatttt attatgtaaa taagaaggat gttcaaattc
     1201 ttgatacata aatccggagg ttgttaataa gcattcggat ttatgtatct tatttggtct
     1261 aggaagtaac ttgattttct tttttcgatt gatccatgag tgcttcttct tttaattcaa
     1321 agatatattc tgagatcctc ataatcactt ttttccagtc ttctggtgtg atgattcctt
     1381 ttaaaatata gatttttttg atatgagaag gcaattgttt caaagggcag tcgctgataa
     1441 cgatgttgca gttctccata aaatcatttt gtattttgat cgattcattt gaaaaaattt
     1501 gttctatctt aacgatcaga tctttactga tataaaaatc acgcgtatag ttcacaccaa
     1561 tcacaattcg cggctgtttt ttgtagcgat catacaaatg tactaggtct tcaaccatcc
     1621 attgcacatt ttccttttct aaaataactg gtaacaagtg ttgattttgt tctctgaaat
     1681 ccatataaaa ttgttcaatg gcggtttgga cttctttcaa tacgctatgt tcttgataaa
     1741 gcatgaaagt aggaaggggc gtatttggtt gattgaagtt aagaaatcca ttataaagtt
     1801 tatttcgtgc gaatgccaat gagtaaagaa tcttttcttt ttcaggtata aacaaatcaa
     1861 atacagtaca aagggactcc accatttctc ttgaataaag aacatgagga acgtttgctt
     1921 tttttaaaag tttgtattgc tccaagcttg tttcttccga ttcgattttc gtgatagaaa
     1981 gtcgagctaa aatattcact aagatgcgtt gttcgcgaca aattggcaga ttaggtcgca
     2041 aaatatctac agagagatca ataaaaaagt caatatattc atactctgtg aattcttttt
     2101 gtacgtcttt cacagaagtt gcaggaaaat tctctttaca tatttttaat agaaaatata
     2161 atttatctat cataccgcta tttagtcggt caagtacttc ttttttaaaa aaagtattca
     2221 tcataaaagc cactacttgc ggatcttcat cataaaattc tgtagcagta cttgaaaaga
     2281 tgttccaata aatatctaat gctgcatatt gaatttgtac ctcttttcca gaaaaagact
     2341 ttctgccatt attagaaaag gagatagaca aaccgtacta tgctaaaaat gcattaatct
     2401 ttttcatttt cgtatacaaa taagaggcac tatagtttgt ttgctgactg aaactgataa
     2461 tcgaatttgt ctgacagctg aacaaagaag tcaagatctg ataactgact gattcgttgc
     2521 agtatcttcc aaatagacga taatagcagt aagccggatc aattccttca aaattcaact
     2581 ttaaaaaagg tttttgctta tcaatatgca gttctgttag attgagacgc tcaaagtctt
     2641 gttctaattc tttgatgtat agtaaaaaat ttgattcaga catatggaaa tgatctgcta
     2701 aatattgtgt agtaataaag aaacgatgtt cagataggaa aagaaaaata tctaattttg
     2761 tattgctttt tttcttcaag ataaacgttt taatcgcttc ttgagtttgc atgaaagagg
     2821 ctccttttta cttaaattgt taaaacttga aaatattact gtttttttca taaatttaga
     2881 caaaaaaaca gtaaacatat aggtagaatc tctactatct tatttcttgc atacaaaaaa
     2941 atcaaggagt gagagagtta tgagatattc aaccatctta atatcaacat ttgcagcctt
     3001 aagcttatcg cttactataa atcagacagt ccacacagag gagacagaaa cagccaaatg
     3061 gacagaaacc aagtgggcca acgtcaatcg aattaccttt caggatgaat tttcatcctt
     3121 caattcagga gtcgcaaaat taagaaaagg acaaacagcg accatcaaag caaaaattga
     3181 ctattcggga gacttagcac ctatactaca atcagctgtt acatttgaga atgttgatcc
     3241 cactttgtcg attggctatg acgagaacac tttgactatc caacacaagc aagcgatatt
     3301 taccttcacc gttacattaa atcaggactt gttaaaacca gcgcttttta ctgtcaaggt
     3361 atcagatgga ctaccgaacg atcatcattt aacgccgtat agtcagcggc agacaattga
     3421 gcaagattca gccatcgact caggaggaga tcttgttgaa gaaccaacag ataaaccaga
     3481 aaatgaaaat aagccagaag taccgcctac agaaaatcca gacggagaac agaaaccgga
     3541 aatagaacct ggagaagaac ccgatacaga aactcaacca gagccagaca ataaatcaaa
     3601 acctgaaata acccctggag aaaaaccaga tgtaactcca gaaccagata cggattctgg
     3661 aaaccaaaca gtaccggaaa caaatccaga cacagataat gaaactgaaa atccagaaaa
     3721 accagaggta gatccagaag aaaaaccaga tgtgactcca gaaccagata cggacgcaag
     3781 agaccaagga ataccagaaa aaataaacaa aaaaacaatt caagaggatg gaaaaaaaga
     3841 gagtaaaaaa tcaaatctag caattctaaa aataaatgaa gagcagctga ataaaaaatc
     3901 acgaatattt gatagcgccc aatcagctga aaccttaaaa agtagcaagg atacaacctt
     3961 tgctgtcctg aaacgaaaaa taaacagcta ccaaaatccg gagagtcaca aaacaaagtt
     4021 atactgtgga gcggaatcat tctattaagt atagcaacaa tgctttctgc aaaaagattt
     4081 aaacaaaaca gatctctcta acatttgaat aatgtctgtg aacgcatttt tcaaataaaa
     4141 agaaatatcc agaaaatgac cccaaaatac cagattgaaa tctgatattt ggggtcgttc
     4201 atactttatc tttcttcata tttattttaa gggatatctg tttcttttgg aagatggttc
     4261 gggatctttt tcactttcaa tacatctaaa aagaaggtaa agatcggaat gccgacaatc
     4321 aagccccaaa cgccaaagaa ccgttcgcta acaagtaaga ccacaaatgt ataaaaaatc
     4381 ggcagttctg ttttgctgga catcaacttc ggattcagca cataagactc aattagatgg
     4441 acaatcaaaa tcgtaatcag gatataaaca acatctcgga tgcctccttg ggaataagct
     4501 atgaaagata gaggaataca agaaatgata acacctgcta ctgggatcag acttaaaatg
     4561 aaaatcatga tcgctaaact cagcagctga tgaaagccaa atacagccaa ggcgattgta
     4621 gtgagcaacg tatttaaaag agcgacaaca aattgagctt ctaataccaa accaaatgta
     4681 ttgacaaact tatcggcaaa atagtaaata tcttgaaaga accaagaaaa ttcacttttt
     4741 aaaaacaatc ttgagaatac aattgttttt ttcttttcaa tcataaaaaa gaaacttaag
     4801 atgaatgaca tagcaaacgc cactgccatt tttcctatat cctgtaaata gccaagcgca
     4861 atcgaagcgc cattttgaag ctgttcaaaa aaatcgtttc gctcaatata agtatggata
     4921 tattgtaaaa caggatctgc atcttttggc tgatgctcgt aaaaattaat aacggactgg
     4981 atcatgtcaa atgtttgatg aataagcatc gggatatact ttgtgaatgc cagatagata
     5041 aggtaaacga tcagtgtata aatcatgata gctaaaacac ctgtagggat tcggacaaat
     5101 cgctgaatga tttgaacaag atgaatcatc aaataagtaa agataaatgt gagcaaaatg
     5161 gttgtaatca tacttctaac taagaaaaga acaaaaacga caaataataa aacagtgaga
     5221 cggcgtattt tttcattcgc caaaaaacgc tgataaacgg tcaaatcgac aacctccaaa
     5281 taacaaaaaa taaacacgtt attcattata tgatggaata tgaaaataag aaagagcaaa
     5341 aagaaaaacc gctaaatgac gttatcttat gagttggttt tcctcataaa atcgttcatc
     5401 tagcagtttc agcccttttt ctggtcttga tgaaaattat ctagcatttt tcgggtcaga
     5461 tgttgcagtg tatccaattc ttcctgttca agtggaagat aagacttgat cttttgtgga
     5521 ataccttctg cttgtttttg caacgcagca ccttgagcag tcagcgtaac atttacgatt
     5581 ctttcatcac ccgtttcacg ctgacgatta atataatttt tttctgctaa cttttttagt
     5641 aagggagtca gcgtaccaga atccagacac aaatcatttc ctagctcttt cactgaagtc
     5701 tttttctttt cccataaaac taacatcaca agatactggg tataggtaag attcagcggc
     5761 ttgaggaggg gagtgtagtg tctaatgatt tctttagagg cggcatataa aggaaaacat
     5821 acttgattaa ctaaatatag atcttccatt tttccacctc cgctgtttta ctatagtgta
     5881 tcatagcgga tttacacaaa aaagtctatt cactaactgc tttcagagta acttctatat
     5941 ttccagctgt agcttttgaa tacggacaca ctttatgcgc tgcctctaat aattcttgcg
     6001 tttcttccag agaaagacct tcgatagatc cttcaatctc tacacctaag acaaagctcg
     6061 tttcactttc tgaataaagg gaaacttgtg cactgacagt agaagcccca gagatatttt
     6121 tttgctgttt gatcaattct aatgcactgt taaaacatgc actgaaacca gctgcaaaca
     6181 attgttcagg gttggttgca ttttcgacgc gtttaccagg agctgcaata ttcaattcga
     6241 atgtacgatc gggattatgc acttctccag agcgtccgcc tgtgttgatc attttcgtag
     6301 aatacatttt tttcataaag ttaccacctt taattatatt ttatacaatt gaattttaca
     6361 caatataatt gtggaaagca aagaaatgga ttggcataag ataaataaga gaaagcacat
     6421 gaaacagacg cgctttctcg aatagcaaac atatacaaaa aatatcagag acaaaaaata
     6481 atctcatcat aaacattaca tatatttctc aaaaagttgt tttctttctt caaaaattac
     6541 ttcacgatgg acctgatgaa gcatccataa ataaccaaaa ggatctctga acattgagtt
     6601 agtcacacca aagtccatca tttctgtcat ttcttgaatc tctacacagc catttcgtaa
     6661 tgctttatta tgtgtgtcct cgatatcaga gacgatgata ttgaaccaga tcgtatttgg
     6721 atgttcagca gtaggagcag tcaaaccaag agacgtgttt tcatccagca tatggaatct
     6781 cgtaccatac aaggtaaaaa tgacttcatt attgcctcta gggtaatctg ttgcctcaat
     6841 cagttgtatt tcatcaaaga ttgtttgata cagttctagt gctttcaaac tatcatttaa
     6901 tacaaaatcg atttccgttc ctttatgcat aaagatctcc tctccttttc agtcgaacag
     6961 ttactagatt cttttattat accagctaaa gaaaacgcat tcaaaaaaat agcggattta
     7021 cttaccaact cattcgattg gatgataagt aaatccgcgt cccattactt ctgctacgct
     7081 gctgacaatg acaaaggcac ttggatcgat ttcttctatc aatggtttca ttcgggaaaa
     7141 ttcatgatca gtcacaacga tcaataaaac ctcttttgac tttcgattat acccgccttt
     7201 tgcctctagc agggtaaggc cacgtccaat ctcagcactt aagcgaaggc ggatgtcttc
     7261 caaataatgt tcactcatga ccataacaga tttctttcga ttgatacctg tttcgatata
     7321 agtcatgaca attgaagtga ttgcaataga taaaatagca taaagaaact cttctattcc
     7381 aaaaataaac agattaagag aaacaacgaa tgcgtctgta aaaaataagc caatcgaagt
     7441 attcaagtgg aaatattttt taaaaatgag gggagggatc gtcgtaccgc cgctagaaga
     7501 gttgttttta tataaaatcg caactcctaa agcaaaaatc gcacttccaa aaatgacaga
     7561 caacaaccga tctgaagtaa tcattgtttc tggaactatt gcaatgatta aaggaaaaac
     7621 caagcttccg aaaagtactt taaaaaaggg ttttttacct aaaaatagaa gagctagtac
     7681 caacataacg atattcaaca ctaaaataac tatagctcgg tcaaagccta aagcagcttc
     7741 cagtaatatt cctagaccgc tgacacctcc agcagcaata tgatgaggcc ctaaaaacat
     7801 attgatagcg actcctaata tgaagagggc aaaaataatc ttaatgtatg tccatataaa
     7861 ttgtctcgtt tctttcattt attcacttcc ttaattcatt aataatactc ttatcttatt
     7921 ataacttact tttcacctta agttaacttt aattaataaa aaaattaggc tagtctaaat
     7981 tattatgttt cattcttcta gaatatactt ctacgccaaa aactgttaaa aaacttttac
     8041 tttagaaaat tggagaaatt gatcgcttaa gataaattca ggacactaaa tagtacagga
     8101 agtagactaa aacttttaaa agttttaagt ctatctattt ggcaagttta aaaaataaaa
     8161 attcggtatc aaaaaactgt taccgataac atttttgaaa gcgttgtctt ttgcttctct
     8221 atcacttata gtgatgacgt catattaaaa cacaaggagg aaatcaatgg atacagctgc
     8281 aatttatcat cgaccggaaa gtgaatatgc atttctatat acgaatacaa gatttcgtat
     8341 tcggcttcgt actaggaaaa atgatatcaa aaaagtatat gtgctatgtg gagatccata
     8401 taccataaca actgaaaaat ggtatcaaaa acaacgacca atgaaaaaat gtctaagtac
     8461 caatgttcat gattattggg aaattgaagt gacgagcgaa acaagtcgct tacagtatgc
     8521 gttccatgtt gttggggaag ataacacgga ctgtttttac ggtgatcaag gaatttttcc
     8581 ttatcgagaa aatgtgatta ctgaaccaaa tttttatttt cgaatacctt attttcatca
     8641 aatcgatcga ttcactattc cagaatgggt aaaagaaaca gtatggtatc aaatttttcc
     8701 cgaaagattt gcaaacgggg acaaagcaaa tgaccccgaa cacactttac cttggggaag
     8761 taaagatcca gaccgagaag actttttcgg gggagatttg caaggtgtga tggatcattt
     8821 ggattacttg acggaccttg gcatcaatgg catctatttt tgtcccatat ttaaagccac
     8881 atccaatcat aaatacgata cgattgatta ttatgaggtg gatcccgctt ttggcgataa
     8941 aaaactttta aaaaacttga ttgatgaagc acataaaaga gggatacgta tcatgttcga
     9001 tgctgtattc aatcatatgg gtgtccattc accacagtgg caagacgttc tcaaaaacgg
     9061 agaaaaatcg atctataaag actggttcca catccgtttt ttcccagtag attcttatca
     9121 gatgactgac ttaccagaaa ctgctgaaaa tatcccatat gatacttttg cttttactcc
     9181 attcatgccg aaactaaata cagcaaatcc agaagtgcaa aaatatttgc tggatatcgc
     9241 aacttattgg ataaaagaat tcgatattga tggatggcgt cttgatgttg ctaatgaagt
     9301 cgatcatcat ttttggaaaa aattcagaga agcagttaca gaaatcaaac cagacatcta
     9361 tatccttgga gagatttggc attcctctca agcttggctg caaggggatg aattccatgc
     9421 agtgatgaat tatgcattta cagattcgat taaagattat tttgcaaaaa agaaaatcac
     9481 agcttcacaa atggtcagtg gaatgaatca tcagcaaatg ctttacagag atcaggtgaa
     9541 cgaaggaaca ttcaacttat tagattctca tgatactgcc aggatcttga ccctttgcca
     9601 aggtaacaaa gaattgatga agtctgtgat ggcctttatg tttcttcaaa aagggtctcc
     9661 ttgtatctat tacgggacag aaatagggat gactggtgaa gatgacccgg attgccgcaa
     9721 atgtatgata tgggagaagg aggaacaaga tctagagcta ttcggtttta taaaagaact
     9781 tgtttctcta aggaaacagt tgtctaagat tatttctgaa ggttctactc agtggctaat
     9841 agtaaatgat cgtgaagata agctctattt cacacggaaa ctagagggac agatcatcta
     9901 tgtttacttt aatcagtcaa aagaaccttg ggtagtagaa caggaaaatg aggtgattct
     9961 tagtcaaaat tgtcagttgt tagaagacgg gaaagcagag atacgacaat acggattttt
    10021 gatcatgtgt tgaaaactgt taccggtaac aacaagaaag cgtttgcaaa tgaagatttg
    10081 tgcccattat aataagggtg taacagcacg ttgaatacaa aggaggaaat gaagatgaaa
    10141 aaaagaagtg tgaaacagtt gggtttaggg ttaatctctt taggaatctt aggcggatta
    10201 gcggcttgtg gcaatggtaa ctcttcagca gaaggggaag ccgatgaagg aaaaacgtta
    10261 acagtttccg ttgattctgg atataaagat tatataaacg aaataaaaaa cactttcgaa
    10321 aaagaaaacg atgtgaaaat caagctagta gaaaaggaca tgttcgatca gttggaatca
    10381 ttagcgttag atgggcctgc tggaaaggga cctgacgtta tgatggcagc atatgaccgg
    10441 atcggtgcgt taggacagca aggacatctg gcggaagtaa aactgggaaa tgaagcagct
    10501 tacgatgaaa cagacaaagc ccaagtgact tatgatggaa aaatctacgg agaacctgca
    10561 gtaatcgaga cccttgtttt gtactataac aaagatttag tcgatacagc accatcaacg
    10621 tttaaagatt tggagaatct atccaaagac agtcgttttg cttttgagtc agaagcagga
    10681 aaaaatacag gatttttagc taaatggact gacttttatt attcctatgg tttaattgca
    10741 ggttacggtg gctatgtatt tggtgatgat ggcacaaatc cttcagatat cggtttgaat
    10801 aatgcaggtg ctgtagaagg aatctcttat gcaacagatt ggttcaaaaa tgtttggcca
    10861 aagggaatgc aagacataaa aagtgcagga gacttcgcca gccagcaatt catgtccaat
    10921 aaaactgcag caatcattga cggtccatgg caagcgcaaa catacaaaga aaacaatatc
    10981 aattatggtg tagccaaaat tcctactcta aataatggac agccttatca accgtttggc
    11041 ggaggaaaag gatgggtagt cagcaattat gctaaaaaca aagacctctc tcaaaaatgg
    11101 cttgattatg taacgaatca ggaaaatcaa gaaaaattct ttgaaatggt aaatgaaatc
    11161 cctgcaaacc aacaagctag ggaaacagca aaggctaaaa acgatgaatt aacaacagct
    11221 gtgattgaac aatatgaaac agctcaagcg atgccaaaca tcccagaaat gggcgaagtt
    11281 tggacaggtg cagaaaactt gatgttcgat gcagcatcag gttcaaaaac accaaaagaa
    11341 tccgcagatg aagcagtaaa aacaatcagt gaagccatag aacaaaaata ctgattaaaa
    11401 tgataaccgt ccatttttat aaaaaatgga acaggtgctt cagataaaag tggcaagagc
    11461 gagatggaaa attatcttgc tcttgttgcc attatcttaa aagcagtttg cggtttagca
    11521 agtttagagg gggcattcag atgcagcaag ataaaacaag caatatcaac gaaaaaaaaa
    11581 tcacgctttg ttcacttatt ccagggatgg ggcagtttgt aaatggccaa gcttttaaag
    11641 gtatcgtttt tttcgccata ttcgtcctat tcattggtca gatgcttctg ggcggatttc
    11701 atgcattggt aggattggtt actttgggaa gtataccaat ggaagatcat tcattattta
    11761 ttttgattga aggcactttg caattcatca ttacaatcat tttcttgatt ttttacggtt
    11821 tgaatctttt agacgcttat cgagtagcga agatgcgtca agagggcaaa aaaatcaatc
    11881 ggacgatgaa ggaagtcata cataatatag gtgatgaagg ttttccttat ctattgacac
    11941 ttcccgctta tcttctcatg atttttacga tcattttccc agtcctagtt acattgttca
    12001 ctgcatttac taattatgac ttcaatcata ttccaccagc cagtttaatt gattgggtag
    12061 gggctaaaaa cttttttagt atcttctttt taagctcata tcgtaatact ttttttgcag
    12121 tcttttcgtg gaccgtgatt tggactttat gtgcaacaac tttgcaaatc acccttggta
    12181 ttctcacagc ggttgttgct caccagaaat ttattaaatt caaacgtatt ttcggtatca
    12241 ttttcttatt accatgggcg gtcccagctt ttattactat catgagtttt tcgaatatat
    12301 tcaacgatag cgtaggtgcc atcaatactc aagtgattcc tttcataaac cagcttcctt
    12361 ttgtagatat cagtgcaatc tcttggaaaa cggacccgaa ctggacgaaa gtagcaatta
    12421 tcttagtgca gggctggtta ggatttccct atatctatgt gatggttaca ggaatcttac
    12481 aatcgatttc agaagactta tatgaagcag caaaaatcga cggagcaaat gcatggcagc
    12541 gcttttcaaa catcacgctt cctgctgtct tcctagtggc agcgccaaca tttattacac
    12601 agtatacggg gaatttcaat aatttctcca tgatctatct gtttaacaat ggcggtcccg
    12661 gaagcgtagg cggaggagct ggttctactg atattcttat ttcctggatc tataaactta
    12721 cgactggaac ttctccgcag tattctatgg cttcagcaat cacgttgatt atctccgtca
    12781 tcgttatctg tgtgtcattg ctggtattta aaaagacaaa tgcgttcaat atgggggatt
    12841 aacaatgaaa aaatttcatt cgagtgctcg attccaaaaa cgactttctc acttttttac
    12901 ttactttttt cttttaatac tgtcagtcat tatcatctat ccgcttttga tcacagcaag
    12961 tactgccttc aagtcaggaa atattgcagc ttttagttta gatctttcaa gtgactggac
    13021 tttaggaaac tttactcggc tattcagtga aacactttac gggacttggt ataaaaatac
    13081 attgatcatt tctgtcctaa caatgatcgt acaagtgact gtagtaactt tggcgggcta
    13141 tacttatagt cgctatcgtt ttatcggccg caaatcaagt ctgatgttct ttttagtgat
    13201 tcagatggta ccgactatgg cagcattgac tgccttttat gtcatggctt tcttattaga
    13261 agcgctcgat aagtactggt tcttgacaat gatctatata ggcggtggaa ttccaatgaa
    13321 tacttggctg atgaaaggat actttgacac tgtaccaata gatttagatg aatctgctaa
    13381 acttgatggg gcagggcatt tcaggatttt tttccagatt gtattaccgc ttgtcaaacc
    13441 gatgctcgcc gttcaagcat tatgggcttt tatgggacct tttggggatt tcatgttagc
    13501 taagttcctg cttcgaacac ctgaaaatca gactgttgct gtcggcttac aaacatttat
    13561 ttctgatgtg acaaaccaaa gagtctctct ttttgctgct ggggcaattt tgattgcact
    13621 accgatttgt ctattattct tctttttaca aaagaacttt gttaccggcc tgacatctgg
    13681 cggaacaaaa ggataggagc agagtatcat gcaaacattt ccgttaaact atttttccgc
    13741 attacgtacg ccaactcaag tgtttgcggg acgcaaacta ttatcatggc ctaaattttt
    13801 cttgatattt gtatttttag tttccttgat ggtcatgcct gtgactcttt tttatgccaa
    13861 tcagatccaa gcgattccat tagaacaatt tctatccgtt cattcgctga tcgatgaaca
    13921 aggaacccaa aaattttctg aacttgaatt atctgaaact gggttacaag catcgcaaca
    13981 aacgatagct gtgacccctg aaattttggt aggtgtgtct ttatctgaga aacaacaatc
    14041 cgatcatgga acttttattg attttgagaa ggaacaatgg gtgatccaac aaaaggataa
    14101 atctggtata cgccgctata cgatgaatta tagcccatct ttccaaccag attctgtacg
    14161 tacgcccgaa gatttccaac gatttttaga aagagaattt tatgccagta atcgtccaac
    14221 aatcatttta tcttatagtt taagtcttgg tcttgtccta tttgtcatga cgagtctcat
    14281 tttattcggg gcttcctttt tcttgtggat gacaagaaaa agccaacttt catccattca
    14341 tacattcaaa gagagtgcaa atttgatgct gaatgttatg ggaataggtt ccatggcagc
    14401 agcagttgta gggtttatcc actttgactt tatcttgatg ctaagtgtcc aaacaacgat
    14461 aactgtcttg ctgcttttat gggtttttgt caaaacaaga ttcaaagata agcgtgtcga
    14521 atgacaagtt taagcgattt atgcgacaat gaatgtgaaa aaaatgaaaa ataaagtagt
    14581 ttttgaaaaa tttctgaaaa aattaaactt cttagttgat tcttttcttt tttagtatta
    14641 acgtaatagg gtagtttttt taaggaggaa aatcatgtta gagaaaactt tatacaatca
    14701 attattttca cgttctttct cgctccctgt cgaagtaaat tattgggacg gtacaacgaa
    14761 acaatacgga gatacggaca ctcctcccca aatcaaaatc aatatccatg aagaaatccc
    14821 aatgaaagag attacaagta acgcatcttt ggcactagga gaagcctata tggatcatcg
    14881 aatcgagatc gagggaagta tccaagcatt gatcaacgat gcttatcagc aagcaaacag
    14941 ctttttacga ggaaaagatt atctaaaatg gctgccaaag aaagaaaaac atacgaaaca
    15001 aaaaaacaaa gaagatatcc atgcgcatta tgacttaggt aacgactttt ataaactttg
    15061 gctagatcca actttgactt attcttgtgc ttatttcaat acacctgaag atacgttgga
    15121 acaagcacaa atcaataaag tccatcatat cttagacaaa ctctttatca aagaaggcga
    15181 aacgttgctt gatatcggtt gtggctgggg aacattgatg ttcactgcta ccaaagagta
    15241 taatgtcaag gcaactggga tcacactaag tgaagaacaa tacgatttga ttaccaaaag
    15301 aataaaagaa gaacatttag aagataaatg ccgtgtatta ttaatggatt accgcgaact
    15361 aaaaggcgaa acgtttgatc atatcacaag tgtaggtatg ttcgaacatg taggttctga
    15421 aaatcttgaa ggttatttca aagtggtaaa agatttattg aaaccaaaag gaacagcttt
    15481 gatccatgga atcagccgac aacaaggcgg tgcgacaaat gcttggatca ataaatatat
    15541 tttccctggc ggttatgtcc ctggtgttac tgaattagtt gataatatta caaaaaacga
    15601 tttgcaactg attgatttgg aaagcttacg tcgtgactac caattgacat tagaacattg
    15661 gacaaagaat tttcatcaag tccaagatca agtcacgaaa gaaatgggtg attccttcta
    15721 tcgaatgtgg gatctatatt tacaagcttg tgcagcttca tttgaatcaa gtaatatcga
    15781 tgtgattcaa tatttgcttg ttcaacctgg aaacaatgac atcccaatgc atcgtgcata
    15841 attgaggcaa aagtactgtt taaagactat ttaaaaagaa gctaagtttt atgatgctcc
    15901 agaagtgaac ttttttgtct aacttctagg attgtctcta aagcttagtt tctttttctt
    15961 tatattttca taataaaagt ttggttcttt aatttttaaa catgacagaa aattttttct
    16021 tcgttatact tgtaatagaa tactatttta taacaaaggg gatcgaatca atgaaaaaaa
    16081 ctgttattta tactagttta tttgcagctt tgtttttagc tgcttgtcaa tcaaatacca
    16141 atcaaggcac atctgggaat acgtcttcca gtagcgaaca gcgctcttca caggtatcgg
    16201 actcctcaac aaaagcaagc acatcttcgt ctagccagac aaaaagttct agtgaagcaa
    16261 gtactcagtc agatatcaaa gtgtctgcag ctcgtgcgat cgaattgttc caagataaat
    16321 acccagatac tgcaattact agtttggaat tagatagtga ttggggatct tacttctaca
    16381 agatagaagg tgtcgatgat caaaaagaat acgaagcaag aattgatgct caaaatgaaa
    16441 aaatggagac agagaatcca gaaactctag atcgagatga acaaaatggt caaaagaaaa
    16501 aagaagatgg tctggatgtt gataaattga tcacgattga ggaagcagca aaaatcgccg
    16561 tcgacaaagt aggaagcggt acggcaacag attgggattt ggataaagat ttaggtacta
    16621 cttattggga agtcaaagta aaagacggca atcaaagtac atcagtcaag atcaatagcc
    16681 aaacaggcga agtactgtca actgaacaag atgattaaga atttcttcgg aaaacaagag
    16741 ttgatttttc ttttagtcat gatacactag acaaaataac ttgtttgaag gagcgattta
    16801 cttgagttta gaaacgacct ttgccaatca tatctttgcc aatcctttga tgaatgcatc
    16861 tggtgtccac tgcatgacta cccaggaatt agatgaactg gctcattcag aagcaggagc
    16921 atttatcact aagagctgta cgatcaatga acgaaaagga aatcccgaac cacgctattt
    16981 tgatgttcct ttagggagta tcaattctat gggacttcca aacttaggtt tttcttatta
    17041 tctagagtat gctcttgcgt atgaaaaagt gcaggaaaac caaaatcaac cgttattttt
    17101 ttccattgcc gggatgagtg tccaggaaaa cttggaaatg cttgagaaaa tagaaaaaag
    17161 tgggtttaat gggatcactg aattaaattt atcttgtccc aatgtgcctg gaaaaccaca
    17221 actagcttac gattttgagg ccacttatga aacactgaaa gaagtattca gcatcttttc
    17281 taagccgcta ggaatcaaat tgcctcctta ttttgatttt gctcattttg atcagatggc
    17341 tgatatcttg aatcagtttc cattgaccta tgtcaatgct atcaacagtg tgggcaatgg
    17401 tttgtatatc gatacagaac aagaagcagt agtcatcaaa ccaaaagaag ggttcggcgg
    17461 cattggcgga gagtatatca agccgactgc tttggcgaat gttcgcgctt tttatacacg
    17521 tttgaaacca gaaatccaaa tcattggtac aggtgggatt cggacaggac aagatgcatt
    17581 tgaacatctg ctatgtggcg caagcatgtt acagattggt accgagcttc ataaagaagg
    17641 gccggaaatt ttttctagaa tcataaaaga attaacgcag attatgtcag aaaaaggata
    17701 tacgtcaatt gacgaattta aaggcaaatt acgcaccatt tcatagcaaa gagacgaagg
    17761 tcatgattga tcttcgtctt tttttataaa aaacactcgt ctgttcattc ctgtgcaagc
    17821 gtactcattg tatgttagac gaacttgtgg gaaaataaca gaggcaaaaa ggaggataat
    17881 atgaatgatg caacagaatt agcaaaagag cttaaagaaa aagaatttag ttttcaagaa
    17941 ctagttacgg atatacagaa aaaaataaag aagcaaaatc atgaattgaa tgcgtttgtc
    18001 gcgcttgaac cattcgagag taattcccac gagctattga ttccccagtc agaaaaaggg
    18061 ccattggctg gtatcccatt tcctttaaaa atgctcggtc aagaaaaaaa gggatggcta
    18121 gctacttcag gatctcgttt atttgaaaca caccgagctt ctcatacatc taattatgtg
    18181 cggcaagcag aagcgatagg tctagttcct tttggacaaa ccaatgcgcc agagtttggt
    18241 ttcaaaaata ttacagaccc agtcatctat ggcccagcaa gaaacccttg gaaccttgaa
    18301 tgtacaccgg gcggatcaag tggaggagct gctgctgcag tttcatcagg aatcgttcct
    18361 ttagctggtg ctagtgatgg cggtggttct attagaatac ccgcttcgtt ttgcggattg
    18421 attgggctga aaccttcgcg agggacgatg ccagttggtc catatgcttg gagaggatgg
    18481 caaggagctg ctattgattt cggcttgaca gtttctatga gagacacgga ggctttattt
    18541 tatgggatga gaagtatcca ttccggtgcg ccatatcaag tatcgccagt tgagtggcaa
    18601 acacatccaa gaaaaaaacg gttgaagata gctttttgtg ttgattcacc gatccactca
    18661 cagattagtg aagaggcagt acttgctgta aaaaaagctg tagatttcct tggaaaagaa
    18721 gggcatgatt tgacagagat tccttaccca ctagatggtc gtaaactgat cacgtcttat
    18781 tatcagatga atggcgcaga aacagcagca atgatccgct cgattgaaac aggcatccag
    18841 cgagctgtca gaaaagaaga acttgaaccc atttcttgga ctttgttcca atatggtcag
    18901 aagctctctg ccgctactta catcgattct ctgcatgtat gggatgaagc agcaattgtc
    18961 atggaaaacc tacatcaaga atacgatcta ttcctttcac caacgactgc atttaccgcc
    19021 cctaaaatca gcgaagattt gcaaagtgat gcgataagag cacagatgga acacatcagt
    19081 gattgttctg aagtagaagg tttagacgtt gtttcttcca tgtttgagga gagcttgaag
    19141 atcacacctt atacacagtt ggctaatctg actggacaac cagcaatcag cttgcctact
    19201 catttgacaa aagaaaacat gcctctagga atccaattta tggctgccag aggaagagaa
    19261 gatttgctat ttcaaatcgg ctatctattt gaagcaaatc atcagttcca tttaccaaat
    19321 ccatggaaat agcagaaaca aggagaagaa gaatgaagaa aaaaagattg tttgctctaa
    19381 taactagtgc cttttttatt ttaagcgctt gcggtacagc tgccgctaat caggaagata
    19441 acagctggag taatgtagaa aaaaagaaag aattagtagt agcgacttct ggcacactgt
    19501 ttcccgcatc ttattataat gatcaaaatg aattagttgg ttatgatgta gatttggcaa
    19561 aagaggtggc taaacggtta aatgtcaaaa tctcctttaa agaatacaac gtggatggac
    19621 aaatcacttc tgtttcaaaa agagaatcag attttgctgc gaacgatttt ggtatcacaa
    19681 aagaaagaga aaaaaaattc tccctttcag agactttcaa atattcattt gacagtatga
    19741 tcgtacgaaa aaaagatgat tcaggaatcc actcacttga agatcttaaa gggaaaaaag
    19801 cagctggcga acccaatact agctatatgc gtttagcgga aaaacacgga gctaaattag
    19861 tcacatatga caatgccacg aatgatcagt atttaagcga tgtagcaaat ggtcgaacag
    19921 atgttattct aaatgattat tatctgcaga aaatggccgt atctgcttta ccagatatcc
    19981 ctgtaaaaat actggagaac gtttatttca atgaaaacga aagtggtttt ttattgaaag
    20041 aaggaaatga tgctctgaaa aagaaaatcg atggcgtatt aaaagagatg aaagaagatg
    20101 ggacgacaaa gaaaatatct gaaaaatatt ttcaaactga tgtttcagtc aaaccgaaag
    20161 aaacgatggc tgagtcggtg agtgagtgat gtactttcct ctaattgatt tttccctcat
    20221 gtggacttca cttcctttcg tactgcaagg cttggtatac acattaggga ttggcttcgt
    20281 gtcgtttctt ttaggaaatc tggtagggtt gcttttgaca gttttaggtc ttttggattg
    20341 gctgcctctg aatgtattta ttcgttttta tttatcgttc tttagaggca ttcccgcact
    20401 agtcctgctg tttctgttat attttgggtt accttatcaa ttaagtgcct taacggcttc
    20461 agtcatctgc tttacgatta ccagcagtgc atttattggt gaaatctatc gaggatcact
    20521 agcaggcgtt tcttcaggtc agtgggacgc tggtttagct tctggttttt ctttctttgg
    20581 ggtattgaaa tatatcgttt tgccgcaagc ttttcgaatc tcagtaccgg cactcagtaa
    20641 cgtagctatg gatctagtga aaggtacttc gttagcagca acaatcacag tgccagaaat
    20701 atttcaaaaa gcaaagatca taggcggtcg gacattcgat tatttttctc tctatatttt
    20761 agttgcactg atttattggg tcatttgtat cgggatcgaa aaagtgcaac agtatttaga
    20821 aaaacgttct atgcatcagt caatctgaaa aatagaaatg gtgctagcgt ggctggcacc
    20881 atttctattt tttgtttctt gtatattctg cctagtattt taaaaccatt tcatgtaaaa
    20941 tgtaaatcga aaatatgttc ggaggtagaa aacatgaaga ccccgctaac catcgaaata
    21001 gaatcctcgt tataccaata ttgcatcgag caaggcggct tggttgtaga agaagtcacg
    21061 atgcctgatg accaaggaat tgtagatacg cttagctgct tgacaaaatt tgatggaagc
    21121 cgagaatggc gatgctatga gctgaaagtg acaaaagcag attttcgctc tacagccaaa
    21181 ctgtcatttg ttggacatta taattatttt gttttaccaa gtaaactata tgaagaagta
    21241 aaaactgaaa tccctacagg aataggtgtt ttagtgtatc gtccgtacac ggttgaagag
    21301 gagatgcctg cctctggtac atttctgatt gcgaaaaaag cagcaaagaa agagcttgct
    21361 gtaccggaag aagctttgac aaaccgcttc atggcttctt tattcaggga agtaagaaaa
    21421 gcaaagcaaa tggagcgagg cgtaaaatat tttccaacag atcaattgta caaagaattg
    21481 aaaagacgtt cagaaaatca agatccattt tctaacgaac gatattatga acggttttta
    21541 gaggaaatag ctagtcaggc aatcaatgag ttgcaagaag aagttatcgc gcttcagcat
    21601 gattatgaat atttgaggca acaacagcag atccgtcggt tgcctacaga gccattggag
    21661 tgaaaaatat gtactatcct tatttaagag gaaaacaatt tgacctatta gcattaaaag
    21721 aagcactgtc gcgaggattg ctctccaata aaatccaacc agtgatcgaa ccagtacgtg
    21781 attccgctac gcttaaaaat gtgatcgaat tatttcaaaa aaagcaccat cgaatcgctg
    21841 tgatccaaaa tccccaagta ggacaattca aattattcga tcagcatgtg cacagttgga
    21901 cagtcaaaga aaatagctct gtcgtccctg cacagatctt aacgccagaa aatctcagtg
    21961 aagtactgga ttcacctcca gcgtttcttg ttttcgatgg acagcactac ccaaaagata
    22021 cagaggtgtg gaaacagttg gcaggagttg attcgaaatt tttgatacca gatactagtc
    22081 gctttcgcat ctggctgccc gaaaacaaaa ttgtgatccg tgactctttt caaacgagaa
    22141 agcatgttga aagttatgca gataaaacag atgacttttt tagtgatgac tatctttttt
    22201 tccacgcaga cggatatatt ggattttctg atttcacgat cgaaggtagc cgttatttcg
    22261 ataaagggtt ccctagccga gcgcttgcta tccatctaac gtatattgat gcttacggca
    22321 atatacgcgt gaaacatttt gtctctgaca gtaacgactc tgctaaagac caagcattga
    22381 agtttcttga agcaggagat aaaatgaaaa aatggatcat gcgtcaccat catcaactct
    22441 tgattacttc aggaatgatt gaattattgg ctctttacca gcaacaaaaa ttccctggac
    22501 tgggagtgtt gaaaaagtgg tcacttatgc atcatttgga acttattagt cacctgctag
    22561 atcatccaaa agattggctt aaatattatt caaaagttca ggaactatat gaagtgatcc
    22621 aatgattaga taatgtaaat gaaaaaggaa tagaaaagag gaaactggat gtatattcgg
    22681 caaacaagga aagaagaagt agaagaagtg gtagaaatca ttaatgccgc agcggatttc
    22741 attgcttcgc aaggctctcc tcaatggcaa aacgggcaga aaccaacaaa agaaacgatt
    22801 ttgaacgata ttgaaagaaa agaaagctat gtattaatag tggaagatca aattgctgga
    22861 acagctgcat tagtctcaag cgtggatccc gtttacacaa aaatagatgg tagttggaaa
    22921 gaatcaactg gtccttattt atcgattcat cgtgtcgctt tagcagaaag tactcgagga
    22981 aaaaaactag caaaaccgtt cttagaacat ttgttgacga ttgcagccag caaaaatatc
    23041 caagatatcc gtatcgatac ttatccagct aatgaaccga tggaaaagac catctattct
    23101 gctggattca cttatcaagg gatgattgaa tttccttttg aacacggcga aagaaaagca
    23161 tatcaaataa tcatcaattg actataaaat catctgagca aagataaaga cattaacact
    23221 actgaaaaaa acaatcatat gatagccaag aggttgtaac agaagtggcc agcttcaaaa
    23281 aacaagaaag aatttccgaa aatgcttctc aaatttttgt ggaattttag cgtattttac
    23341 gaaatagctg ctttctgaaa ctcccttcat cgagaggtcg tgacatagct tttgtcacga
    23401 cctctcggct tatcctacga gtattattcg atacatttat atcaataatc ctttttttat
    23461 tttatctgca gcttcttgga acgctccatc gtttgacacg actaaaagtg aggggatagc
    23521 tttcaaacgt ttcaactgtg ctaaatcttc tacataaacc gccagtctgg ctgaaatctg
    23581 ttcagatgaa tctccacgtt ttttcaggcg cgattgcaat gtttctttag agatcgtaat
    23641 aaagatagga cggactaccg tcccgaaata ctcatataaa tgccaaaaac catttgctgt
    23701 caaaacggcg taacaatttc ctactgtcag tttcttttta atttctgctt tactggaacc
    23761 atatttatgt tgagcatatt ggtcccactc tgcaaactca tttcccttga tcatctgatt
    23821 gaatgtattg tcagaaacaa aataataatc ctcttgatct ttttccccag gtcttggtga
    23881 tcgattcgtg taagtaatga tcttttgttc cggctgaaaa atagcttctg cgagtgaagt
    23941 ttttccagaa ccagaaggac cgataaatac atagcaataa ttcattgtca tttctccttt
    24001 gatgattgaa caagtgaaaa agctttggaa aatatttcca aagcttttaa aagtaacttt
    24061 gcttttttgt atattatacc agcaagccag tcacaatagc acttaaaaag ctgactaaag
    24121 tcgctgttag caaaattttc aatccgtgtt ttgcgacaag atttcctttt tcttcattta
    24181 atcccttcat cgcaccagaa atgatcccga tagaagagaa gttggcaaat gaaacgagga
    24241 agacagatat aattgctgtt gtacgctcag taaaatggaa attaccattt gctaattctg
    24301 tcatagcaac aaattcattt gtaaccagtt tagtcgccat gatactaccg gcatccactg
    24361 cttctttcca tgggattccg ctgatgaagg caagaggggc aaagatgtaa cctaggatct
    24421 cttggaaagt gatgccgaat atcccgtgga agatagcatt gatcatggca atcaaagcaa
    24481 caaaaccgat aagcatagca gcaaccgtga tcgccacatg gaaaccatca agaatgtatt
    24541 ctcccaacat ttggaaaaat gtctgttctt tgttttcttc aatgatcagc tcatcttctt
    24601 tttcttctaa tacataggga tttacaattg aagccagaat ataaccgcca aataagttca
    24661 aaacaagggc agtgatcaca tatttagaat cgatcagtgc catataagat ccgacgatcg
    24721 acatagaaac tgtagacata gccgatacac tcatggtgaa caaacgtttc tcagatagga
    24781 agggtaattc cttttttatg gaaataaaaa cttccgattg tcctaaaata gcagaagcta
    24841 ctccattata agattccaat ttgcccatcc cgttgatttt acttaatcct agaccaacag
    24901 ctttcataaa taatggaaga atcttgatat agcgtagaat accgataata gcagaaataa
    24961 atacaattgg catcaatacg gaaagaaaga aaggtgcact tcctttattt gctacaccgg
    25021 caaaaacaaa gtcaacacct tgcccagcaa aagcaagaag atgatcaaac atttttgcca
    25081 atacagaaac aaccgctgta ccaaaagttg tacgtaataa tacgaaacca agaacaagct
    25141 gtaaaacgaa catcacaatt agtggtttca atttgattgc ttttttgtta ctgctgatca
    25201 aaaaagcaag ccctaatgtg acaaataaac caataatacc taataaataa ctcaaataat
    25261 cactcctatt ccaaattccg ttttatttta cctgttttgc aaataaaata ctaatcttta
    25321 gtgaaatttt cattactttt ttagagtgaa acgcattcat tttccattag aaaataaagc
    25381 gaacaaaata aaaggtttaa aaagtgtaac gtcttagtaa agtctataaa aaaacgaaac
    25441 tatcgattaa gctaatagtt tcgttttcaa catttataag taaacttttt ctattggtaa
    25501 tatagatttt cggaagggta gacaggtgcg caggtaatat cgattcctag ttttttcaat
    25561 gtttgttcat catttttatt gaccatcaca gtagagtggg cttgtgctcc ctcaagttca
    25621 gctagtttat cataagcaac ttgagcgatt ggattcgtta ctgcactgat tgataaagcg
    25681 atcaagactt cattgatgct tagtgcatcc aaagaggaat gaagtgtctt tgatttcaat
    25741 ccttgtattg tttcaagaac taatggtgac aataaaggaa tgtcatctgc gatatgggct
    25801 aaatgtttta ctgcatttaa gataactgcc gctgacgcat ccatcaatga acttgtacgc
    25861 ccggtaatga tgacttcatc tggtaattcg attgcaatca cagcagaagg atcttttgta
    25921 ggactttgcg cttgaatctt atctgcatag tcatgggcag ggactaccac gtttcggtct
    25981 tcaggacgta aaccgacttc ttccataatc agtttgattc gattaactgc ttcttcgtcg
    26041 atcaatcctt ttttgaagtc gcattctgtt tggaaataac gacggatgat ttcttgttta
    26101 gaggcttctt ggacaacggt atcatccacg atcccaaatc ctacacgatt cacacccatg
    26161 tcagtaggag attgataaac agattcttta ccagtaattt tttcaataat acgtttgatt
    26221 acaggaaatg tctcaacatc ccgattatag ttcacagcta ctttattgta ggcatcaaag
    26281 tggaaggaat cgatcatatt gacatctttt agatcgactg tggctgcttc ataagcaatg
    26341 ttcagcggat gtttcaacgg aacattccat actgggaaag tttcgaactt ggagtaacca
    26401 gctgcatgtc ctttttggct ttcatgatac agttggttca aggaagtagc gagctttcca
    26461 cttcctgggc caggagctgt cactacaacg attggtttag ttgtttcaat atactcattt
    26521 tttccaaaac catcttctga gacgattttt tctacgttga ctggatagtc ttcgatttct
    26581 gagtgtttat agaccttgat gtttcgtctt tctagtttat tgataaagac tttcgtggca
    26641 ggctgtccac tatagcgagt gataacgaca ctgttgatta ataagccata accacgtaat
    26701 tcatcgatta accgcaagat atccatatca taagtaatcc cataatcacc acggatctta
    26761 ttacgttcaa tatctccagc atagacgcaa atcagtattt cagcttgatc tttcaatttt
    26821 tgcagcaatt tgattttcgc atcttcttca aatcctggaa gtacacgttt cgcatgcttg
    26881 tcgtcaacta gcttcccacc aaattctaaa tataatttgt catagtggtt cactcgttcc
    26941 aaaatatacg ctgactgctc ttcaatatat ttttctgaat caaaaccaat ttttttcact
    27001 aggttccctc cttaagtact aaagcatttt ttcatttttt tgaggattcg tcaactggaa
    27061 gagcgaaatt cctcaaaaaa acaaagaaaa tggtttaaac caacttgtct agatagttat
    27121 tcattggtat gatagttttt gataaggaag gtgctacatg aaaaaaagac agaaaaagaa
    27181 aaacgcatat aaacactata tccgttcgat ttttaccggt tatgaaagaa tgcttgaaga
    27241 tcccgaattg gaacaattga cgtttactta tctaaacgaa gaaacgcagc taactcgtga
    27301 tgagcatcag cgcattcatt tcacaacaag agatctgcct agtaaataaa aaataagata
    27361 aaggtcatcg gattcgattc tgtcctatca gatggaaaga ctagttgatc gaaaaaaagg
    27421 ctgtaccaaa ttttggtgca gcctgtttta tggagcattg cccgctcttt tttataacaa
    27481 ttttttacag aaaatggtac tattgaagta ttaataagtg aaaaacagat gagtaagagg
    27541 aggcaagtag tatgaaagag ccagatacaa atgcaaatta tgagccagac gaactagagg
    27601 agacgaatgc ggatcaggat acaggacaag aatcgtcttc tttttacgat atcaaagaaa
    27661 aagcaagcat gaatctttcc caattattag aagacttaca ggcttttgaa aaagctgtcc
    27721 gcgaagaaga tatccctgag atctaccgtt tatacaatgg ccgattgaat gaagaattaa
    27781 aaagaagctc aaatgaaaat cacgagatcg atcaattgct gatgcgtaag atccatgatc
    27841 gttttttaca ggaatttcct tttatggaac aagtagaaaa catctcgcca acgatgagct
    27901 attacaaaat cggcacttat taccatgatc ggcccacggt cggtattgat gcaagtctgc
    27961 cagaaatttt tgttttgcct aagatcgatg aagagtggga gaaatattcc caaccggagt
    28021 caacggacta tgacaaaaaa atcaatgagc tagacgcaaa ggtgatcact gctcagacag
    28081 aaattgaacg tttagatgaa caaataaaag aaatcaatcg acaaatgact gatttgaacg
    28141 acaataaagg attcttgaat cgtaaaaaaa tcgaggaaga aattcaagaa ttagagaaga
    28201 agaagcaagt actatctaat gaaaaattag gctggcttcc ttatattgag acgcctgaaa
    28261 caatccagca gcaaaaagaa gcattgaagc aagaagcaag agcagaccag cttcgtgcag
    28321 ccatcgtaga aaaagaacaa aggcaaatca gacgctattt cggtagcaaa gagggattcg
    28381 gtcaagcaat ccatgaattc ttgatgaatt atcttggcaa tgagaatcct aataatcaat
    28441 ctaatgaagg aggttccgag tatgaatgaa cacgatgaag cagaatggac aaaaaatgaa
    28501 gaacaaacag aagatcatga agaaacgaac acttcattgg aagaacaaac aagtaatcct
    28561 ctggatgaca cagaagaaat ccctatgcta gaggaaacaa aaatacacga aaaagaaaaa
    28621 gtaactccga cgaatacgaa tactgcagaa aacacccatg cttacgaaat aactgaaaat
    28681 cccgggtatt tagaaggaaa agagcaagat catcaagata tcgtcagttt gattgaaaca
    28741 gcggaagcag aattagcgga tttacgtatg agaaaagatt tgatcgaatc agattttcca
    28801 caattattag aatcatatga tcgttttata ttgaatgaag aaccaatctc cacgatcgga
    28861 aagacaagcc tgatcgaata cttcacgtat gaattactga aaccgttgaa tgatattggt
    28921 ctggaacggt caaattcagc ttatgacaca tggaaaacaa gacatttttc gatcaattat
    28981 acgttgaatg gacaaaacga attagtcttt tcctttgtat taccgaccat cgatcaacga
    29041 taccaagtgg aatcgatgag tctcctaaac gtatcaccag agacaatgga aatcaatgtc
    29101 caagatgacc gggtgctttc tttgatccga cactggtcag tggatcgagt attctctgca
    29161 gggcagatca cgatattcaa tcacaaattg aatcaattat tagcccatgc aagaacactt
    29221 ggttttaccg ttaatcagac attactagac aatacgaagc cacttcgttt gaatcttcag
    29281 agcgaatttg aattgacgaa tgaagtattg gatgatattt ttatcgtggc aatgaaaaat
    29341 ccatcctacg atatggaaaa gatcaacgaa actacttatc aagtacgatt agataaagga
    29401 cagtctttaa cgataacaac aaacgaaaaa gatcaaacag tcttgtcgat ctcatctgga
    29461 gactacccac gatctgtcat tgatttcttt atcaattacg aattcttggt cccattaatg
    29521 gtgagaaagt cctgattgga taagttggat taccagatgt cattcagata attctgttcc
    29581 aaaggacttg cggcagttat ccagaataat tgtcacaagt cttttctgta tgtactaaat
    29641 ggacgtgaat aacttgctga tgaaggaggg atttcattag gaatgtccac aaataaaata
    29701 gcacagctta gcttattgtc tgctgcttgt gtagcaggta ggatcgcttt tcaatttatt
    29761 ccgaatttcc agccaatgac tgctattttt ttatttatag tactttactt aagcttaaaa
    29821 gatgcgttaa ttgtcatgag tctttctatt gcgatcagca gtttttattt tggtgttgga
    29881 ccatgggtca tcggccaatg gatcagctat actgttgttc tgagtttgtt ttcaatcgtc
    29941 tgcttgagaa aaacagtgca aaggaatcta tggttaaagg gagtctgttt ttttctggcg
    30001 ggcatggttt acggaatagg tatgaccgtt tttgatacgc tgttatatcg tttgcctcaa
    30061 ccttggatct attaccttca aggggtgtcg tttgatttga tgcacagtat aggcaatgtt
    30121 gtgttctttc ttgtttttct gccagtcgtc aaacgttttt acaatcattc aggaggaaaa
    30181 aatgaaaaaa ataatttata gtttgcttgc aatgagtgtc agtatactca ttttgacaag
    30241 ttgcagtaca acctatacac aatcggcttc caaccaaaag gaggaagtaa aaacatcgca
    30301 aaaagcaaca atcacgttac aagaaaatgg tcaaaacttt tcggaaaaag aagtgttctt
    30361 caaaaaaggc gatgatttac tgagtatttt gaaacgaaat tttgaagtca aagaagataa
    30421 cggcttcatc acttcgcttg aagggcactc acaggatgag aaaaatcaac tctattggat
    30481 gtttactgtg gacgggaaaa gtgctactac tggtgccaaa gagatcaaat tgaatgatgg
    30541 acaatcggtc attttcaatt tgtctaaact ataagtttca taaaaaactg ttaaagggcg
    30601 aatattgcca taattcagtg agttcagttg atgaaagaag gaaaataaca tggaaaattt
    30661 taaaaaaagc ggaatgtttt ccgtaattgc tgctctaggg gctaatattc tagttgctat
    30721 cagcaaattt gtcggctatg caatcagtgg cagtgccgcc atgctaaacg aaagcatcca
    30781 cagtatcgtt gattgcagca atcagatttt gctgttattc ggtgataaac aagccaataa
    30841 aggacagagc gagttgcatc aatttggtga aggacgagca aaatactttt tcagtacaat
    30901 cgtcgcaatg atgcttttct tcggcggtgg ggcacttggg gtgatggaag cttttgaaaa
    30961 gttgacgcat ccatcccatg aagtgggtaa tccgatcatt gttatagcaa tactgttatt
    31021 tggtttggta atcgaaggaa gttcgttacg cgtggcaatg aaagaaatca aagaattgaa
    31081 tactgaaaaa ctttccacga tgcgcttttt gcgcgaaagt cgacacagtg aaatactgat
    31141 tatttttacg gaagacttgt gtgctgtaat cggtttggtc ttggctttag caggaacaat
    31201 attgacttat gcaaccggga ttgctgcatt tgatgcgatc agcggattgc tgatcggttt
    31261 cttgctgatg ggagccgcca ttttcctagc aaaagaattt tatagtctga tcatcggtga
    31321 aagcgtgaca gcaagcgatt tatcaaaaat caagttagct tttgaacgtc ctgagatcag
    31381 tcggctgatc gatataaaaa cagttcattt aagtccaacg gagattttag ttgcagcaaa
    31441 agcagatatc gttggtaaca aagagaatga agcttactcg gtcatcaatg atattgaaaa
    31501 aaatatgcgt cagtctcttc ctgacaagaa gatgtatata tacattgaaa cagataaata
    31561 tgacccaaat tattctcgta aataaaacgt ttattcatga attgaagcaa aaatgagaaa
    31621 aatataatct tgtttttaaa taaaacggct acaatttagc gaatttatgt tacaatgagt
    31681 ccgctgttta tttgttgtaa ttaaaggaga tattttaatg aacaaaaaga aaagtgcgcc
    31741 atggcttcaa gctttgcatg cagtgatgcc actttgtata agctatatac cagtaggatt
    31801 agcttgcgga gtattgttgc aaaaagttgg atttgatcct ctcttatcag gactgttatc
    31861 gattctggtc ttttccggtg gcgcgcagtt tttagttgca tctatgctta cgacgcaagc
    31921 atcatttgca acgacattat tgatggtttt ctttttagaa ttacgctata cattattagg
    31981 agcaagtctt gccggtttta tgaaaaaaga aaagcggacg tttttggctg tcttttctca
    32041 atcactgaat gatgaaaact atgcggtgaa ctacttaaag ttttcaactg acccttcctg
    32101 gaataaaaga aaggctttat atgtcaattg gttctcaatg actgcatggg ccggtagcaa
    32161 tatgatcggt aattttttcg gctcggtgat tcgagtagat gctgaccttg tccattttgc
    32221 tttgacggcc atgttcattt tcatgtttat catgcagatg aaaagcgcac tactgatctt
    32281 tacaggttta ttctcaggcg tattaggggt cgtattcatg gtattattcc aaaatacttt
    32341 cggattgatt gcagcaacag tcattgcttc atttgccgga tttatgctgg agaaggcatt
    32401 caaaaaagaa aaagctaaga aactaagaga ccgcggtaaa gatgtcaatg agccgctctt
    32461 caacttcccg gaacaggagg ccaatcacca tgactaaact atatttgcta cttttagttg
    32521 cttgtctgtt catcgtggca tatatcccgc gcttgttccc gatgctttat tttactcatc
    32581 gaaaagtgcc atcctggttc agtgactgga tgaaatatgt accggtggca ttatttgcgg
    32641 cgttagcttt taaagatgtt ttcattactc atgaacatct ggatattgct tggaatatca
    32701 aaatagctgc aatgattcta gtagcaggcg tggcttacaa aacacgttcg atggcgttgt
    32761 cagttttgac aggacttgcg tctgtctttt tgctgtctat gctttaaact gttttatttt
    32821 attaaaaaca gagattgcga cataagttgt tcaaactgat ctatccgaac tgcatttgca
    32881 acagtcaatt ctggatgcaa gtttattcgt agggataaaa aagattagtg ttggcttatg
    32941 atgcaagctc ttttttattt taggaattag agagggattt gtccttctat gattttttgc
    33001 tataataggg aagatgttta ttttatgaaa gaatagttga gggaatttaa atgaataaaa
    33061 aattgatgca aggaacattt tggctaacct ttgctaatct tctatgtaaa gttttagggg
    33121 tagtctattt gattccctgg ctaagtatga tggggaacaa tcaagatggt atgcttgcta
    33181 cgacattgta taatgttggc tatcttcctt atggattgtt cctgatgctt ggtacagtcg
    33241 gatttccaaa tgcgattgca aaaaaagtag ctgtagccac aaaagaagga gataacgctg
    33301 cttgtcggct gatttttaga agtacgatca atatcatgtt tgtcatcggt atcgcctctg
    33361 ctgcactgat gtatttattt gctcctttac tagccggaat cagtccgatt gctaacgtta
    33421 ataatgggat actagctatc agaagtttat gcccatcttt gattgctatc ccaattctta
    33481 gtgcaatgag aggatatttc caaggaaaaa atgatctgcg gccttatgga acttctttga
    33541 ttatcgaaca ggctgtgcga gtgatcgtta tcttggctgg tacttattac ttgcgcgtat
    33601 tgactgacgg aaccatatta gaagctgtat tgatcagtac agtcgcttct ttcttcggtg
    33661 ggttagctgc gattatccat atgtatatcg tcggacgcag aaaagacttc ttcgaactta
    33721 aagatttttt gatttccagc cgttatttcg aaagagaaaa ccgatctgcc tctgtctcta
    33781 tcattcggga aacactaccg tttatttttg tcggctcagt cattactatc gttcaaatga
    33841 tcgaccaagt aacgatgaaa ccactgcttc actttttccg tccagagatc gcagatcagc
    33901 aactggaatt cttattcagc cgtgcttctg tcaatccaaa taaattgaca ttgatcctga
    33961 tctccatggt aggaacagta gctataagca gcttgccaat attaagcacg atgaagaaaa
    34021 aagatcggat acaaatcgaa aaaacagtag gagatagttt ttcgattgcg ttattgattc
    34081 ttcttccttc attgactgga atgtctttat tagctggacc actatacaca ttgttttttg
    34141 gctatgatcc tgaaagtgtc ggatatttcc aaatggcttt gctagcttct ttgtttttct
    34201 ctttattcac gatcctatct acgatgcttc aatctttgaa tcaccatcgt tttgcgatcc
    34261 gattaactgt agaagcaatt atcctgaaag tcatcttcca agtaatcgga ttaggattag
    34321 ttggcggata cgggatgagc ttatcaaatg gcttagcatt cggtattgtc tttgttcgcg
    34381 gctatcactt tatgtgcaaa gaataccgaa tagcaccttt agcaaagatc agcaattttt
    34441 tcttaaaaac tttccgctcc accctgatta tgcttgcagt atgttttgcc gttttctttt
    34501 tgctgaatca gcgattgtca atggaatcaa aaagctatgc aatgatttat tgtgtggctg
    34561 taggaagtat tggcggctta gtatttttat tttctcaatt cggaaaaaat gggtttcgaa
    34621 tgttgaaaaa tgttaaacat catagcagaa gcaaagaata ggttaactaa tcgttttttg
    34681 ctgtcacttg aaaaacggca actcgtatga ttacgcttgt cgtttttcta ttttccaatt
    34741 ttagtttcat atctaatctt acttatgata tagttataag gtctggaaaa attctttgaa
    34801 acgaggaaat taaacacatg aaacttgcag tagttacaga tagttcggca tatctgccgg
    34861 aacgtatcaa gaaccatccc gacttattcg ttattccaat ccctttgatt atggatggga
    34921 aaatttataa cgaaggaatc gatatcgaag cggatgaata ttatggactt ttaaaaaaca
    34981 gtaaagattt tcctactact tcacaacctg tcgtagggga agttttgggt ttatatgagg
    35041 aattgaaaga gaaagggtat gacacgattt taagtatcca tctttcttca gggatttctg
    35101 gctttgtcaa cacattattt gcgatgaaag atgacatcag cggagtaacg gtcattcctt
    35161 atgactctaa gatcactagt atgccgatgg gacacatggt agaagctgcc ttagacttaa
    35221 atgatgaagg tcagtccctt gatgaaatac ttgcacatat cgatcgaatc cgagataata
    35281 cctacgctta tctgattgta gatgatttga acaatttagt acgaggagga cgtttgacaa
    35341 atggtgcagc gctgattggc agcttattaa aaatcaaacc gatcctgaca ttttctgaag
    35401 ggaaaattgt tttattcgaa aaaatccgtt caagtaaaaa agcatttgct cgagctgaag
    35461 aaattattgg tagaagaaat aacgaaatac aaccgccagt caaactttac gtgatacatg
    35521 caaataattt agaagtagcg attgcggaga aagagaaatt gcagaaaaaa tatccaaatg
    35581 cagaaatcga aattggtcat ttcggaccag tcattggtac acatttaggt gaaaaagcca
    35641 tcggcattgc catttcagcg caataattcc ttttattcaa aaaacatacc aaaaatattc
    35701 caacatcatc ttggactttc tacatgttac taatgagcgt gtaaaagaac gagatggtgt
    35761 ttttatttct atcaatgaag ctctttttct ttgcaatcct cacttcaatc ctctataatg
    35821 tcgcatatag tttagtaaat gagtaaacta ctaatttact tatttactaa aaataaaagg
    35881 atgaggagtt aaaataatgg aaataccaac aaatttaaaa cataaaccag taatcgttgt
    35941 agaagactat gataaagttg atggaagaaa tgcattaaat acggatgcaa aagggctttc
    36001 actgggcctt gctcagtgga atgatcgtgg aaaagtcgat atctcaggca aggtatggcg
    36061 acatactgga gaaaaatggt cacgacagtc ggaagaatta ccaatcacac gtattttaga
    36121 tttagcgatc ttagctgcac aaggaagttt atacttccaa gatgcttatc gttatgaaaa
    36181 attttatgat ccagaaaatc cagtcgtaga catcattgga ttacaaggga accgaatgac
    36241 ggtagaagta gataccaaaa atccaaagat cgatgaagac atcattttgt attacgatac
    36301 actacaaaaa gacggagaac tgattgggga acgattccgt attttaaaac gtcttttaga
    36361 tgagctagga tattaataga cagagatgat caaaagggga agagaagcat ggagaatttc
    36421 tcactaacag aaaaccgaac cttttcacgg ctagaaaagc aaatgatctg gaagaaaccg
    36481 aaaagccatc ggacaagtgc agaagaatgg cggatcgcct ccgaaatcaa agggaattgg
    36541 caagatcctg aaatgaaaat cagtaacgta cttttggaag gagatgccgg gtcggggaag
    36601 acacaattag ccaaagcact atctgctgat ctgcaacttc cctatacaaa agtgacctgt
    36661 tttgcagata tggacaaatc agatgttttc ggagccttat tacctgtagt agattctaat
    36721 aaggaacaag accaagaatt actagaagct atttaccaaa cagatacact tgaagctgta
    36781 ctgacactga tccagaacca ttacggcatt gatccttttt ctgctaaaga aaaattagca
    36841 gagctagttc aaaggatcga agctgattat ccagatgcaa tccattacag ctattaccct
    36901 tctgaaatcg ttcgagcgat tgaaaaagga tatttattag agattcaaga gccgactgtc
    36961 atccgagatg cctctgttct tgtggcactt aactcagcgc tcgaaccaaa tgggatgttg
    37021 aatttaccga caggaatcat tcgtcgccat ccagattgcg tcattgtgat cactacaaat
    37081 cgaaattacc aaggaaatcg cccattaaat gaatctttaa gagatcggat gcaacatgcg
    37141 gaaaaaatgg atcttcctgc cttagaagtc atggcagaac gtgcgatggc gaaaactatg
    37201 atccaaaaac aagaacttct cttaaagatg gcagagatta tccgtttgtt ggatgaaact
    37261 gcaaaagcta acgccatcaa aggtgtagca ggaatgcgtt cttattttta ttgggcaaat
    37321 acctataagc aaggacaaga tttactccag tcgatttatc caaaagtttt gtataaatta
    37381 tccactgatt cagatgaatt ggcaattctt gaacaagctt tgtctgacag tggattgcta
    37441 ctggaacttc aggacatatt gagaaaagaa aaatggggtc atcctcaatc tgagcataca
    37501 aaaggaagaa ccattagcgc cgaagaagca aatgaacgaa acatcactga aaatgctgaa
    37561 gaaattctgc gcttagatga aaagaaatcg ttgtctgctg aagaaccaaa tgagggagaa
    37621 gagaaacaag aagaaacaat agaaaaaaac gagattcctc ctcaaatgca aaaagaacgc
    37681 agtagcgata tcgaaggaaa aaatcaaaaa gaacaacaaa caggagatga ttctcaagaa
    37741 atgtctgctt ctgacatgga agcacatgat aaggaaatca agaagcaact gaataaagaa
    37801 gcgcgacaaa tcatgaagga aacgattcat gaaaaagaag gtctcatcgt tcaccgacca
    37861 gctttccaag gaaaaacgcc ggaagcacgt gagctacaca tgcaagtaat gccaatagta
    37921 gaatcactta gccggcaaat tttagagtta ctggataatg aacaaacaga gacgtaccaa
    37981 aaaggaaaat acgaaggtca gcggttcaat gcaagcaaag tagcatatgg tgatttgaga
    38041 aattttgaca aaaagaaccc gccacatgag caaccttctt tagcggtagc tgtacggatc
    38101 gatgaatctg ggtcaatgat acgggatgat cggatcgaag cggcaaaaaa agcagcgatt
    38161 gccattgccg aattttctaa gaaagtcgat atcccattgt tgatttacgg agatacagca
    38221 gaccgttcgt caagagaaaa gacttctttg ttttcttata aagaatttga agacggattt
    38281 caatggttaa atgaaaaatt agtcactatg aaaccaaggc aaaacaatcg tgatggtgct
    38341 gcacttcgtt tagcagcaga aaaattaaac agacaatccg caacaaccaa actgttgctt
    38401 aatattagtg acggtcagcc aaaagcgttg ccggattata caggcaaaaa agcaaaagaa
    38461 gatatccagt ctgttttgaa agaatatgaa cgtcaaggaa tcctatttgt ctccgcagct
    38521 attggccaag acaaagaaga aatcaagtcg atctacggtg aaagtcgttt tgtagatatt
    38581 actgacttga atgaatttcc caagcaaatg attcaactga ttgcccgcta tctatgataa
    38641 aataaagctc aacgaacgca cttagcagat tcgttgagct tttcgtttct agttaccggt
    38701 aacaaggata cttctaaaat agaatcggtt ataatcaaga gggaccgtta aaggagtgaa
    38761 acgatatgat acaagaacaa aaatggtgga aagaagcagt aggctatcaa atctatcctg
    38821 ctagtttcaa agatagcaat ggcgatggtc aaggagacat caatgggatc cgtgaaaaac
    38881 ttaattattt gaaagatctt ggtatcggct ttatctggat caatccgatc tatcaatctc
    38941 catttgtcga taatggttat gacgtaagtg actatcaaga tatagaagag agattcggaa
    39001 cgatggaaga ttttgattta ttgctaaaag aagcacatga attagggatc aaagtgatta
    39061 tggatcttgt catcaaccat agttcggatc agcatcaatg gtttgaagaa tcgaaaaaat
    39121 ctaaagaaag cccgtatcgc gattattaca tttgggtcga cggggtagat ggcaaagaac
    39181 cgaataattg gacatcgatt ttcggcggtt ctgcttggga gtattctcat gaaactggac
    39241 agtattatct gcatgttttt gcaaaagaac aaccagactt aaattgggaa agcgagaaat
    39301 tgaaagaaga actattcaat atgatccgat ggtggttgga taaagggata gatggatttc
    39361 gtttagatgc gatttctcat gtaaaaaaag atgaatattc agtaaaagcc acagagaacc
    39421 ctttctcacc ctttcaaaat gtctcaggaa tcgaagaaca tttgaccgat ttgaaacatg
    39481 tttttgaaga gtacgatatc atgactgtcg gagaagcaag tggtgtcact gcagaagaag
    39541 gaccacaatg ggtagggaaa gacggttatt tcgatatgat attcgaattt gatcatatcc
    39601 atatctggca gcaagaaaaa gaagggcaat tagatgtcct taaattgaaa cttgcattaa
    39661 gcgcttggca aacttcattg gatggtatcg gatggaacgc tttatatatg gaaaaccatg
    39721 atgttcctcg tgccgtttca gtgtttggag atactcgtcc cgatttttgg gctatgtctg
    39781 caaaagcaat cgctatgatg tatttctttt tgcaaggtac gccatttatt tatcaaggac
    39841 aggaaatcgg aatgacgaat atgccctttg aatcgatcga tcaagttgat gcagtagatt
    39901 cgaaaagact ttataaaagg ctgcttgcgg aaggtaagac aagagaagaa gcattggata
    39961 tcattcgtga aacaacgaga gacaacagca ggacacccat gcaatggact tcggaacaat
    40021 atgctggttt ttctacacat gaaccttggc tgatcactaa tccaaatacc aaaacgatca
    40081 atgtagaaca acaagaatat gaaccagagt cggtcctgca attctataag aacatgatcc
    40141 gcattagaca aaccaataaa ggtctgatat acggttctta taaagaatat cttcatgaac
    40201 atccacaact ttatgtctat gaaagatatt tagaagatga acaatatctt attatggtaa
    40261 acttaacaga atcacttgct gactatgaac ttccaaaaga agcagatcag tcctggacat
    40321 tactcttgtc aaattcctct agcggagaat ttgaagctaa aggcatacta gcaccttatg
    40381 aagctcggtt atataaaaca aacaagtaaa acttcctcgt taaagttcat tagttggact
    40441 ttgcgaggaa gtttttcaga ttgctctgtc aattactcat ttcaataatt cgctctagga
    40501 ccaccgatat atttaggacg agagcgaagg gctgtcacgt gagcttgtcc aagggttttc
    40561 aaacgaggtc tgacaggtac ttggacatgt ttgatatgca taccgattga tgtgtcaccg
    40621 atatcgatac cggcttgtcc aactacgtgt tccacttcga caggatcatt gaactggtca
    40681 aaagcagcaa cagaacaagc acctccagca tgtagtgcag gaacaacaga gacaatttcg
    40741 aaattttttg cttctgccac tgttcgttct actactaatg ctcggttcaa atgttcacaa
    40801 ccttggacag ctagcgcaat ttctttagga tccaacaact cttttaacgt acggatgatc
    40861 cattgaccaa cttccgcact ggaatttttt ccaatatgtc ctccaacgac ttcgcttgtg
    40921 ctgcatccta aaacaaaaag atcacctttt ttcaagtgtg cctcagccaa tatgtcatta
    40981 actatttcag tcaattggtc ttttaattct ttttctgaca acttcatttt ctaaaacctt
    41041 ctttttcaat taatattgct tccgtgcttg aatacgttga agacttgcag ttataggaaa
    41101 ggcaagtccc attccaatca gattttggaa gatgtttcct ggtatagaag ccactccagc
    41161 cggccaatca tataaaatcc aaccggcgat accatagccg atgatcataa ctaagcttcc
    41221 taataaaagc cctaatcccc acatacgagt agaagatttg ctgaaagaac cagcaacata
    41281 tccttgtaac ccgtggatca ctaatgaaaa aactgcccac tgcggatatc ctgaaatcaa
    41341 atcgatcaat agaccactgg aagcaccaac aactaatcca cccgttggac cataaagaaa
    41401 agcagcggta tagatcccgg cctcgcataa tgtcacaaat ccattcgtag ccggtacagg
    41461 gatcaaaatt gttaatgaaa gggcaactgt caacgcaatc agcatagcat agatcgttaa
    41521 ttgcttagtt ttcatttctc attcctcctt tttgaatacc gccagaccag acacttccgg
    41581 tatgatcgtt catcagcacg ccattagaaa tacaatgggc aacaaatttt ttactttctg
    41641 ctactgcttt ggtaaaatct gcccctaaca caagattcgc tgttattgca gaagaaaagg
    41701 cacaaccggc cccgtttact gttgtagtgt cgattttttt atgagaaaat acctcataat
    41761 gatcaattga atagagaaga tccacagctt catctccagc catccttgtg ccgcctttga
    41821 tcacaatgtt ttttgttcct gaagcagtga gttctttagc gagatggatc atttctgtta
    41881 acgaagaggg aagagcttga ccagaaagta aagcagcttc tgccaaatta ggcgtaatca
    41941 tcgtaacaag tggaaaaagt tcttcgatga gctgcgaaat atacaattga ttgatcacag
    42001 cttcagtttc tttgaatgcc aaaacagggt ctaaaatgat tgggatattt tgttttgttt
    42061 ttatgaagtc tcgaacaacc atgattgctt cacttgaatt caataaccca attttgatac
    42121 catctaatga agtcatttta tcaatcgaaa ctaactgtcg tttaagcaat tccaaagata
    42181 tagattccag cataaatcgc tcattaatat ctagtgcccc tatagaagtc aaggcgctta
    42241 gtccgaatag accaaaattc tcaaaagttt ttaaatctgt ttggattcct ccaccagcaa
    42301 atggatcaga accgccaatt gtcaagattc ttttcataac attttcctcc atgaataaga
    42361 gtataacgaa attttcaaaa aagaaaaaca gccaattggc atatttttat ctcatccaat
    42421 ttaaaaaaac aaaaagaaaa gaaagaccaa aatggatctt tctttcctta aatagttagg
    42481 acttttgtga ttttaattct ttctcgattt gaagccgttg cactttcatt gtagctgttc
    42541 gcgggattgc atcaaaatca cggatgatag ggacgttcaa gcgaggaagt tcatggactt
    42601 gctcccacca tcgattccaa tccatttctt tatcaggagc taaagcaata atcggttgtg
    42661 gagaatgatt tttatccctt acaatcacta cttctgccaa aaaatctaac gaatctaata
    42721 aaaagtcttc aatagctaat gtactattga tcgtttcgat caaatccact tgccgatctt
    42781 tcaaaaatag gtggccatgc ttatccataa aaccgtaatc tccgctatcc caccaatcgc
    42841 cataaacatt ttcctggaac cgtgcatctt ccttgtaata agtcaaagcc cgtcctttag
    42901 acagtaattg tatatgacca tctgtgtttt caggtaggac attcccaact gaatctgtaa
    42961 tccgagcgct tgtcatgtct tccagtccaa cgcccatatc tcttgcatca cttgttttca
    43021 aactctctag tgtatgagcc ttaagaatca ttggtccgca ttcactttgg ccatatactt
    43081 gaagaaatac cgcatcctgt gccatcgacg ttcgcaaaaa tgtcttcatt gtctggttgt
    43141 ttatagcatc aaaagtggag tgatagtatc ggatacccga aaaagcttct ggatgctcct
    43201 tagcagtaaa acgccactga acaaagttat tcggatgggt ctctagtgca attggccggt
    43261 gagcttccaa catatgaaca acttttgaac tttgtgcgtt tgctaaaggc atcataggaa
    43321 atcccatgga catcaaagaa gagataccaa tattgaatct ggaatgcact ggagagatgt
    43381 gaaaagcgat taatttcttt tccgaaattt ttgtgaaaac tgttttttgc catttcgtcc
    43441 gccatcccat agaatgagca gaatgacaaa taagtttagg gataccagtt gtaccagaag
    43501 tatgggtcat ataagaaatt tcatttttgt ctaaaaatga ttgagaaaca gacaaagcag
    43561 gttgttccat taaataaaga ataggaa
//
LOCUS       DANJMI010000023        41707 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000023 DANJMI010000000
VERSION     DANJMI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.023"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(12..617)
                     /gene="dhaL"
                     /locus_tag="PWU81_001456"
     CDS             complement(12..617)
                     /gene="dhaL"
                     /locus_tag="PWU81_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDL1110164.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="PWU81_001457"
     CDS             complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="PWU81_001457"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL1110165.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1630..2076)
                     /gene="dhaM"
                     /locus_tag="PWU81_001458"
     CDS             complement(1630..2076)
                     /gene="dhaM"
                     /locus_tag="PWU81_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL1110166.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2179..2499
                     /locus_tag="PWU81_001459"
     CDS             2179..2499
                     /locus_tag="PWU81_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDL1110167.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2611..3480)
                     /locus_tag="PWU81_001460"
     CDS             complement(2611..3480)
                     /locus_tag="PWU81_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL1110168.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(3576..4880)
                     /locus_tag="PWU81_001461"
     CDS             complement(3576..4880)
                     /locus_tag="PWU81_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL1110169.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4877..5386)
                     /locus_tag="PWU81_001462"
     CDS             complement(4877..5386)
                     /locus_tag="PWU81_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110170.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5561..7696)
                     /locus_tag="PWU81_001463"
     CDS             complement(5561..7696)
                     /locus_tag="PWU81_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1110171.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7779..9434)
                     /locus_tag="PWU81_001464"
     CDS             complement(7779..9434)
                     /locus_tag="PWU81_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL1110172.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9436..9750)
                     /locus_tag="PWU81_001465"
     CDS             complement(9436..9750)
                     /locus_tag="PWU81_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL1110173.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9920..10180
                     /locus_tag="PWU81_001466"
     CDS             9920..10180
                     /locus_tag="PWU81_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDL1110174.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10194..10508
                     /locus_tag="PWU81_001467"
     CDS             10194..10508
                     /locus_tag="PWU81_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDL1110175.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11238..11792)
                     /locus_tag="PWU81_001468"
     CDS             complement(11238..11792)
                     /locus_tag="PWU81_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDL1110176.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11918..13847)
                     /locus_tag="PWU81_001469"
                     /pseudo
     CDS             complement(11918..13847)
                     /locus_tag="PWU81_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14153..14869)
                     /locus_tag="PWU81_001470"
     CDS             complement(14153..14869)
                     /locus_tag="PWU81_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL1110177.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="PWU81_001471"
     CDS             complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="PWU81_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HDL1110178.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="PWU81_001472"
     CDS             complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="PWU81_001472"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL1110179.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18339..19799)
                     /locus_tag="PWU81_001473"
     CDS             complement(18339..19799)
                     /locus_tag="PWU81_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1110180.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19789..21084)
                     /locus_tag="PWU81_001474"
     CDS             complement(19789..21084)
                     /locus_tag="PWU81_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL1110181.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21416..22093)
                     /locus_tag="PWU81_001475"
     CDS             complement(21416..22093)
                     /locus_tag="PWU81_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDL1110182.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGDH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22123..23214)
                     /locus_tag="PWU81_001476"
     CDS             complement(22123..23214)
                     /locus_tag="PWU81_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL1110183.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(23315..23749)
                     /locus_tag="PWU81_001477"
     CDS             complement(23315..23749)
                     /locus_tag="PWU81_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL1110184.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24333..25790)
                     /gene="ascB"
                     /locus_tag="PWU81_001478"
     CDS             complement(24333..25790)
                     /gene="ascB"
                     /locus_tag="PWU81_001478"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL1110185.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25787..27682)
                     /locus_tag="PWU81_001479"
     CDS             complement(25787..27682)
                     /locus_tag="PWU81_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL1110186.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27740..29515)
                     /locus_tag="PWU81_001480"
     CDS             complement(27740..29515)
                     /locus_tag="PWU81_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL1110187.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29565..31061)
                     /locus_tag="PWU81_001481"
     CDS             complement(29565..31061)
                     /locus_tag="PWU81_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL1110188.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31255..32877)
                     /locus_tag="PWU81_001482"
     CDS             complement(31255..32877)
                     /locus_tag="PWU81_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL1110189.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33205..34518)
                     /locus_tag="PWU81_001483"
     CDS             complement(33205..34518)
                     /locus_tag="PWU81_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL1110190.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(34763..34987)
                     /locus_tag="PWU81_001484"
     CDS             complement(34763..34987)
                     /locus_tag="PWU81_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110191.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35007..35429)
                     /locus_tag="PWU81_001485"
     CDS             complement(35007..35429)
                     /locus_tag="PWU81_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDL1110192.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(35571..36281)
                     /locus_tag="PWU81_001486"
     CDS             complement(35571..36281)
                     /locus_tag="PWU81_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110193.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(36299..36796)
                     /locus_tag="PWU81_001487"
     CDS             complement(36299..36796)
                     /locus_tag="PWU81_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1110194.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(36833..37285)
                     /locus_tag="PWU81_001488"
     CDS             complement(36833..37285)
                     /locus_tag="PWU81_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL1110195.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(37420..38025)
                     /locus_tag="PWU81_001489"
     CDS             complement(37420..38025)
                     /locus_tag="PWU81_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL1110196.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(38475..39095)
                     /locus_tag="PWU81_001490"
     CDS             complement(38475..39095)
                     /locus_tag="PWU81_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL1110197.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39097..39966)
                     /locus_tag="PWU81_001491"
     CDS             complement(39097..39966)
                     /locus_tag="PWU81_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDL1110198.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(39989..40396)
                     /locus_tag="PWU81_001492"
     CDS             complement(39989..40396)
                     /locus_tag="PWU81_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL1110199.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(40415..41704)
                     /locus_tag="PWU81_001493"
     CDS             complement(40415..41704)
                     /locus_tag="PWU81_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1110200.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        11759 a         8982 c         7022 g        13944 t
ORIGIN      
        1 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
       61 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
      121 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
      181 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
      241 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
      301 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
      361 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
      421 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
      481 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
      541 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
      601 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
      661 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
      721 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
      781 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
      841 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
      901 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
      961 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
     1021 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
     1081 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
     1141 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
     1201 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
     1261 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
     1321 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
     1381 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
     1441 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
     1501 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
     1561 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
     1621 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
     1681 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
     1741 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
     1801 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
     1861 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
     1921 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
     1981 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
     2041 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
     2101 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
     2161 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
     2221 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
     2281 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
     2341 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
     2401 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
     2461 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
     2521 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
     2581 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
     2641 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
     2701 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
     2761 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
     2821 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
     2881 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
     2941 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
     3001 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
     3061 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
     3121 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
     3181 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
     3241 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
     3301 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
     3361 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
     3421 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
     3481 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
     3541 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
     3601 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
     3661 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
     3721 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
     3781 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
     3841 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
     3901 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
     3961 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
     4021 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
     4081 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
     4141 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
     4201 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
     4261 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
     4321 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
     4381 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
     4441 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
     4501 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
     4561 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
     4621 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
     4681 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
     4741 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
     4801 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
     4861 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
     4921 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
     4981 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
     5041 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
     5101 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
     5161 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
     5221 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
     5281 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
     5341 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
     5401 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
     5461 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
     5521 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
     5581 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
     5641 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
     5701 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
     5761 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
     5821 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
     5881 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
     5941 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
     6001 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
     6061 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
     6121 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
     6181 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
     6241 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
     6301 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
     6361 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
     6421 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
     6481 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
     6541 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
     6601 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
     6661 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
     6721 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
     6781 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
     6841 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
     6901 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
     6961 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
     7021 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
     7081 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
     7141 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
     7201 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
     7261 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
     7321 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
     7381 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
     7441 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
     7501 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
     7561 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
     7621 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
     7681 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
     7741 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
     7801 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
     7861 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
     7921 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
     7981 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
     8041 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
     8101 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
     8161 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
     8221 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
     8281 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
     8341 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
     8401 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
     8461 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
     8521 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
     8581 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
     8641 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
     8701 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
     8761 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
     8821 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
     8881 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
     8941 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
     9001 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
     9061 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
     9121 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
     9181 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
     9241 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
     9301 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
     9361 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
     9421 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
     9481 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
     9541 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
     9601 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
     9661 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
     9721 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
     9781 acctcatggg gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat
     9841 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
     9901 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
     9961 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    10021 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    10081 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    10141 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    10201 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    10261 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    10321 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    10381 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    10441 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    10501 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    10561 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    10621 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    10681 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    10741 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    10801 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    10861 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    10921 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    10981 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    11041 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    11101 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    11161 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    11221 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    11281 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    11341 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    11401 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    11461 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    11521 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    11581 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    11641 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    11701 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    11761 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    11821 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    11881 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    11941 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    12001 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    12061 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    12121 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    12181 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    12241 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    12301 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    12361 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    12421 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    12481 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    12541 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    12601 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    12661 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    12721 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    12781 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    12841 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    12901 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    12961 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    13021 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    13081 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    13141 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    13201 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    13261 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    13321 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    13381 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    13441 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    13501 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    13561 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    13621 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    13681 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    13741 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    13801 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    13861 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    13921 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    13981 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    14041 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    14101 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    14161 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    14221 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    14281 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    14341 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    14401 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    14461 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    14521 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    14581 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    14641 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    14701 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    14761 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    14821 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    14881 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    14941 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    15001 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    15061 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    15121 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    15181 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    15241 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    15301 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    15361 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    15421 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    15481 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    15541 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    15601 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    15661 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    15721 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    15781 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    15841 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    15901 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    15961 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    16021 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    16081 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    16141 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    16201 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    16261 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    16321 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    16381 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    16441 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    16501 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    16561 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    16621 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    16681 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    16741 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    16801 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    16861 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    16921 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    16981 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    17041 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    17101 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    17161 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    17221 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    17281 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    17341 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    17401 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    17461 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    17521 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    17581 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    17641 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    17701 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    17761 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    17821 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    17881 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    17941 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    18001 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    18061 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    18121 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    18181 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    18241 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    18301 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    18361 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    18421 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    18481 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    18541 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    18601 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    18661 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    18721 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    18781 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    18841 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    18901 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    18961 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    19021 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    19081 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    19141 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    19201 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    19261 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    19321 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    19381 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    19441 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    19501 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    19561 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    19621 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    19681 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    19741 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    19801 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    19861 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    19921 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    19981 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    20041 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    20101 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    20161 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    20221 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    20281 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    20341 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    20401 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    20461 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    20521 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    20581 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    20641 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    20701 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    20761 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    20821 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    20881 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    20941 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    21001 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    21061 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    21121 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    21181 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    21241 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    21301 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    21361 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    21421 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    21481 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    21541 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    21601 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    21661 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    21721 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    21781 ttgttccgtg gtcaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    21841 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    21901 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    21961 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    22021 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    22081 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    22141 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttttt caaaaggttt
    22201 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    22261 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    22321 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    22381 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    22441 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    22501 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    22561 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    22621 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    22681 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    22741 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    22801 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    22861 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    22921 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    22981 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    23041 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    23101 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    23161 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    23221 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    23281 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    23341 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    23401 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    23461 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    23521 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    23581 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    23641 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    23701 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    23761 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    23821 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    23881 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    23941 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    24001 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    24061 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    24121 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    24181 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    24241 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    24301 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    24361 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    24421 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    24481 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    24541 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    24601 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    24661 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    24721 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    24781 accccttccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    24841 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    24901 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    24961 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    25021 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    25081 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    25141 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    25201 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    25261 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    25321 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    25381 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    25441 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    25501 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    25561 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    25621 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    25681 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    25741 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    25801 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    25861 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    25921 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    25981 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    26041 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    26101 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    26161 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    26221 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    26281 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    26341 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    26401 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    26461 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    26521 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    26581 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    26641 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    26701 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    26761 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    26821 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    26881 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    26941 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    27001 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    27061 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    27121 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    27181 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    27241 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    27301 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    27361 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    27421 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    27481 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    27541 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    27601 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    27661 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    27721 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    27781 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    27841 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    27901 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    27961 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    28021 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    28081 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    28141 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    28201 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    28261 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    28321 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    28381 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    28441 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    28501 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    28561 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    28621 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    28681 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    28741 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    28801 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    28861 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    28921 gcatgatatt ggaaaaaatt gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg
    28981 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    29041 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    29101 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    29161 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    29221 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    29281 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    29341 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    29401 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    29461 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    29521 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    29581 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    29641 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    29701 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    29761 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    29821 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    29881 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    29941 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    30001 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    30061 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
    30121 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
    30181 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    30241 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    30301 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    30361 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
    30421 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    30481 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    30541 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    30601 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    30661 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    30721 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    30781 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    30841 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    30901 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    30961 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    31021 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    31081 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    31141 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
    31201 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
    31261 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
    31321 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaatagtga acgtctgctt
    31381 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
    31441 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
    31501 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
    31561 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
    31621 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
    31681 tttcgccttg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
    31741 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
    31801 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
    31861 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
    31921 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
    31981 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
    32041 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
    32101 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
    32161 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
    32221 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
    32281 gtccatacga aagcccccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
    32341 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
    32401 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
    32461 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
    32521 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
    32581 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
    32641 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
    32701 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
    32761 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
    32821 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
    32881 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
    32941 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
    33001 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tatgcaggga
    33061 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc cttggtatta
    33121 gctcaaaaaa cggcaaaata taagaaacta ttttttcgtt tttttctcta atactcaggc
    33181 cagaacgctg atttcacaac ctctttattt cacattcaat atccacatga atcccacaaa
    33241 tacaaagaac agaccataca tgattggaga tacttctttt cctcttttag ctgctaacat
    33301 ggtaattgga tagaagataa aacctaatgc aatcccatct gaaatactat aagtcaaagg
    33361 catgccgatc aagatcaaga atgaagggat cgcaatctct aagtttcccc attcgatttg
    33421 tcgcaaagat tgtgccatca atactcctac cataatcaat gctggtgcag ttacttgtga
    33481 agtaacaact gatagtaatg gagaaaagaa caagcctaac aagaagaaaa tccctgtagt
    33541 aatagctgtg atacctgaac gtcctccaac ggcgatacca gctgatgatt ctacaaatgc
    33601 accaacagga gaagtaccaa ataaggaacc cgcaagcata gctgttgaat cagaagctaa
    33661 agctttccct acacgaggca tcttgttgtc tttcatgaaa cctgcttggt ttgctaatcc
    33721 aactaaagtt cctgctgtat caaagaaagt cacaagcaag aatgtcagca ctacgaccca
    33781 catttgaaga gaattgatgt cccctacatg ttttaatgca actaaaaatg ttggtttcaa
    33841 acttggtgct gcggcaacaa tttcagaagg cattggaatc aaacctgtga ccagacctaa
    33901 aatcgttgta gctgccatcc cgatgaagat gccacctggt acacgccgaa caagcaagat
    33961 agcagttacg actaatccaa aaatcgtcaa ccaagtagaa ccaacgttga gtgatcctaa
    34021 tcctactaat gtagattcat tcgaaacgat gatcccacct tcgctcagac caaggaaagc
    34081 gataaataga ccgataccgc cagaaatcgc atatttcaaa tctgctggga tcgcatcaat
    34141 gatcatttcc cgcagcttga agattgtgat caaaataaaa atcaaagaag caacgaatac
    34201 gccggctaat gctgtttccc atggaatccc cattcctaaa caaacagaat aagcaaaaaa
    34261 agcattgatc ccaagagcag gtgccgttcc aattggataa cgggctaata cccccattaa
    34321 aacacagccg atcgcactgg ctaatgcagt cgcagtaaac actgctcctt catccattcc
    34381 agaagcacct aatacagttg gatttacaaa taagatataa gccattgaga taaatgttgt
    34441 aaatcctgcc agtacctcac gttttaccgt ggtgttcaat ttttctattt cgaagtagga
    34501 aagtattttt tccttcatct tccatccccc taaatccgaa cattataaat aaagtccgtt
    34561 aaaatttcga tttattaata aaacaacgtg ttttatcata tactaaaaca aaacataagt
    34621 aaacaaaaaa ttcggatttt aagcaaaatc cgaacgttcc atctgattaa atccctttat
    34681 ctcaaagttc atgaacattc actttggatt acaagaattt ctttattgtg ttaataaagt
    34741 ttcttcattc tagtctcaaa aatcaatagt gtgatttgat tttaccattt cgtttctgtt
    34801 cttctttgat gaattctttt gtatcgacag gattcagatt atttagtccg ccaaaacctg
    34861 tatcgaccat ttttccccag ccttttgtct tcttttttcc tgatttgaga ttctttgctc
    34921 gaagcttctc actatgtttt gctcgccgat acttcaaata accttctaat cttcccatta
    34981 gatccactgt ttttcctcct ttttattcaa tcagacgact cagcttttcc tataacaaaa
    35041 taattgcctt cagggtcaga aaaattgaaa gtcaagatgc cattgttatc tgtgatttct
    35101 cctggttctt ccatgttctc gtagatagtt tcaaattcat ccgtaaaaaa tactaaagaa
    35161 ggcggtggtc ctaatacctc tggtgaaaac ttctgcacaa atacttttgg gaatacgcct
    35221 aattctaatt cttttgagat gctgagtaca atactcttaa attcttctgg caattcgatt
    35281 gtttcagaaa tttctgcacc aattttttct acaaagaatc ggcaaatcat ctctacgtca
    35341 ttgacataaa gcatcacacg tgttttttgt atcatcattt gacccttctt tctatttgat
    35401 tttctttcct gtctataaac tgattccatt atatcacgaa agcgcaaaca ccttgtgttt
    35461 gatactattc aagaaaaata aagcaatagt ttctaggatt gctttcctcc acaaactttt
    35521 tttacaaaaa aaaaacggca atgaatgccg ctcttcttat ttcgattctt ttataattct
    35581 gcttctgatt catctttggt tagtgtgtgt ctaacactag ttacgccaaa tgtttgatcg
    35641 attttttcct tcaacgcttc gattgcctgt gcgccacgtg ccgcttgttc agcattgatg
    35701 gattcaatga ccagcaccgc tgcttgtgtt tcttccgtca gcatcgttct ttgggcttca
    35761 cgccagttga gacagcgaca gatcgctcct tgttcatcat agtagatgat ttcttccgga
    35821 agtgccggag catcggaatc tgcacctaaa ggcaggaatg attcgccacc ttgggctttt
    35881 cctaaatgca gatttcctgc gatcttttcg atatcttctc cgccgcaagg tacgccaaaa
    35941 gacaaggaaa tactattata gatatctaca agcggattga ttgggaaaaa ttcccgttct
    36001 tgatttgcac gtttcagtaa agcttcaata gaagaacgtg cacctttttt agtcttgaat
    36061 gtggtaaatg cctgacgcca ctgagcaatg acttcgtttt ggctgaatgt ttcttctgta
    36121 aggtaatttc ttgcttcttt tgctgcctca ttcaataatt gatgatacgt ttcttcactt
    36181 tcttttgtta cgtggttatc gataccattt acggataaga tttcgatggt tgcttcagga
    36241 aatacttccc agaaattggt atccactatt acttttttca tctgttcaca cctcttgatt
    36301 agaataattg cttgatgaat agatagtctg tttggggatc atctcccata taaaaaacat
    36361 gttcgctgta tttgataaat ccttttttac gataaaaagc gagcgcaaca tcgttgtgtt
    36421 cccaaacacc gagccaaatg gctgtttttt gcttttcttt tgctttttca atagctgtat
    36481 ctagtaaaaa tgtaccgata ccttgatgtt taaatttttt tcgtacatag attcgctcga
    36541 tttctaaaaa atgatcagcc atttcctcag attgactcgt tcctgtattt agtttcaggt
    36601 aacccgcagg ctcttgatcg atactcacta ggaaaaaatc ggtttcttta tcttttagct
    36661 cttctgtaag tttttctagt gcataagctt gtgtcaagta atcagctaaa tgctccggtg
    36721 aattatcttt tccaaatgta tctgtaaatg tttcgatact aatttgttgt agtaagtcta
    36781 catctgaagg cgtcattttt cgaatttcgt atttattcac ggcttcttcc tttcaataga
    36841 tccttttatt gccttttttg acaaaattcc aatcttcgct aacattcttg catactttat
    36901 ttaaaagata ttccaattgt tttgcttctt cgtcagaaag accatttaat gcgactgcat
    36961 tggagtagtt gttttctcga atgatgaaag gatagatttc tgctcctttt ttagtcggat
    37021 agagtttctt gattttttta ttttctttat cttccaaacg ttcgatcatc ccattggatt
    37081 ctaatttctt gattgctcgg gcagtcgttg tacgatctac tttgatcatt tctgctagtt
    37141 tttcttggat gattccagga ttttcgcaaa tacgtactaa ataaagatac tgtcctcttg
    37201 ttaaatcgac ttctttgaat tcgatattgc taatagaatc tagcgctcgg gcaatcattc
    37261 cgatgtctcg aaggatttca gccatttcat cccaccttta ttttgtttgt aagactagtt
    37321 cacctctttt tgttgcaaat gcaactattt tcaataaaaa aatcaagttc ttttttaata
    37381 actagtaaag tatattcttt tcttctttta cgaaaaagct caatcacaat agatctggtt
    37441 tttgttttct aaaataagta actgtttctt cgttgttcgc ttttctgata gctttggcaa
    37501 ctcttctata ctaaaaaatc ccatgttgga agtttcatta ttctcaataa acttggcctc
    37561 ttcaccgtgg atcgcacaag caaaaacaag tttataatac tgaaaaagct gtggaatatc
    37621 tttttgttta ttcgtatcaa aaactgctcg caactgagta gtttctactg ttaatcctgt
    37681 ttcttcatag acttctttac gcacattttc ttcaggagac aatcctattt cagcaaatcc
    37741 accaggaagt gaccattctt tcgtccgttt atcttcgatt agtaggacct tgccatcttt
    37801 tttgatatag gcacgaacat ctacttttgg tgttggatgt ccttctccaa tcgttgtgag
    37861 cgcttttatt tcttcgaaag tttcttttcc tatttcactg atcagttcca atgaaatcgt
    37921 ccggatctcc tcaaaacgct ctttatcaaa tacatctttt ccataatata gaccagcttg
    37981 agaaagtgcg agtagtcttt tgtattgagc caacttcttg ttcattctct tcccttcttt
    38041 tttagcaaat gtatgactat gtaatcaagt atagaagact ggtttaatta cggcaaataa
    38101 atataatgaa atctgcaata aataaaagaa tgttaaaaaa ctcgagaaca aaagacattc
    38161 gacttctctg gggctgtgaa taaatggcgt tcaaaagctt gcatctgcgg taataagccc
    38221 caccaaacga aaaatatgac gagctattct tgtctggctg ttcttattaa ttgaggcagg
    38281 acagcttttt cacaacctcc ccactaaacg gtgttctcct accaactcct cggtcacaag
    38341 tcacaatatt catcaaacat gagaagctgt ttttttgaaa ttgctccttg tgactcggag
    38401 ctgaacgaca ggctcacgac ctctcggtaa acagcgttca aaagccaatt tttcgatatt
    38461 aagccatttc tttgttactt attgtcattg atttcttttt ctaatttatc caatagtgga
    38521 gtgtaatcca cttgagtata gtctgttaca tcaaattcat atagtgttcc taactggaat
    38581 ttgtcatatt ttcggtcggc gatgatcttt ttgaatgctt ctttcgtaat atgattggtc
    38641 cctttcgttc gatcttccaa tacgtcacca tagtgataat gatccatgat atagctcaaa
    38701 tgacgatctg attcacgatc acgtttgtac cgacgttgcc ataacacttc aaagtctgct
    38761 gttaggcgaa tcgtgatcac ctgatatccg tatctatctg ctaattcttg gagtcgtgct
    38821 ttttgcttat cactaaaagg atactcggac acaatcactt tctttcctgc ttccatatac
    38881 aaatcaagtg ctttgtaata gaaaggataa actttcgtcc tttcttgttc cgcccgttct
    38941 tccaaattgt taaacccgat ggattctgca tagtattcct tgatttcatc gagcgtgatg
    39001 gcatacatat ctggaaatcg ttcccgtagt ttattcataa gatatgtttt tcctgtacca
    39061 ggactaccag ccagaagaat gaaatacttt ttcatctcat ttatttccta agttaattgg
    39121 actttccaag ttcataaaca gtccaatctt atgcttgaca atttctttta ctgcttcatt
    39181 tgctggaatc acattatggc gaagagactt gttgatttct gtttcactaa aggtttcttt
    39241 agctactttc gtccagttga ctaaaagctc cgttccaaca ttgaatttgc ggatcccgtt
    39301 attgatcaat tcaggataat cttcttcttt cacacctgtt cctccatgga taactagggg
    39361 gacatcaact actgcatcga tttctttcag caagggaatg tttacttctg tttttgattt
    39421 gaattgtcca tgattcgtac cgatagcaat agctaatgca tccacgcctg tagcttctac
    39481 aaatttcact gcgtcttccg gtcttgtata taccttgtca ttttcagcga catggattcc
    39541 ttcttctgta ccgccgattg ttccaagttc tccttctacg gaaacaccgt gcgcatgggc
    39601 atattctact actgcttttg tcttcaggat attttctttg aaaggcagat gggacccatc
    39661 atacatgaca gaagtatatc cgctgtcgat tgcttctttg atatcatcaa agtctcgggc
    39721 gtggtctaag tgcaagactg catcaaccat ttcttcttct gcaatagctg tcattacatt
    39781 gactaacaca tgcatgccga tatatttagc ggtatcgaca cttgtttgaa taatgatcgg
    39841 cgaacccatt tcttttgctg cgcctagcat agcaggaagc atttctaggt tatgcgtatt
    39901 aaaagctcca actgtaaaat tcaactcttc tgctgttttt gttacttctt tcaatgttgt
    39961 atacatcttt tattgccacc ttatcttttt atttgtttcc tttgatcgtt acttgttttg
    40021 caatttgtcg catctcatcc attttgccca tgtagtaatc gatcccttga tcatcaccag
    40081 cacgagcagc atctgcacgc ttctggttat agaggctcat cagtttttta tacccatctc
    40141 cgatcgtctg tttattttcc aaactctttt ctaaatattg gatggcttga tcgttcacat
    40201 acaattcaga aaatttaagt ccaacttgtt cataaagttt cgctaattta tgcttatctt
    40261 gggtagtgga aatctcttgt ttcaattgtg taatctctga ttgaatcttt tcggtcgctt
    40321 ctggcgatag ggctttttct tcttttgcca gaactttttt cggttctttt tcttttttct
    40381 taaaaaatcc aaacatcatt ttccctactt tcaattaaaa tgctttcaaa actgggctta
    40441 agatccaggc atataagagt gctgcggcta ctgtatctac atcggttgct gtagcattgt
    40501 tgaatcccat cgaattaaag atcgttacta gcaaagcggg caataatgta ataaagaaac
    40561 catgagcaat accgccaatg atcgctcctc ggcgtccgcc aactgcgttg ccaaatatcc
    40621 ctgctgtccc tccggcaaag aaattggtta gcataccagg taaaatcatc gctaagccga
    40681 acatgggcaa agtaaacatg gcaataatcg taccaatcgt tgtcgtaatg aaacctaaaa
    40741 tgacagcatt tgggctataa ggaaagaaaa ctggacaatc taaggcaggg atcgcatcag
    40801 gaactaattt catagctatc cctcgaaatg ccggcacgat ttcacctaac agtagacgta
    40861 caccagctag caatacatac acacccacta cgaattggat ggcttgtaaa aaggcaaaca
    40921 tgatatagtt ttgatctcct gataattcag aagcgaatgg ttcgcctgca aataaaacag
    40981 tgatgatata tagcgggacc atcacgacca tgacagacaa ataagtatct tgcagaaatt
    41041 cgaaatgtgt cggcaacttg atatcttcga tcgacttttt attttcgcct ttctcaccaa
    41101 aaagcttagc aacaccggct tcaaataagt acccgatcgt acaaaaatga cctaaagcaa
    41161 tatcatttga tccagtgatt ttacgaatga tcggttgcgc tactgccggc atagcaatgg
    41221 cgaaacaagc tccgataaaa ccaccgacca agatcaatac gatccctcgc agaccggcaa
    41281 aatagccaaa gactgtcgtc atcgttgcca tccataaaat tgcttgacca gttaaaaaaa
    41341 tgtacttcca ttttgtgaaa cgagccaaaa taatattgaa tacaaaaatg gctaagaacg
    41401 tcagtgcaat atctctacct aaacccaaat cattcattgc ttgaccattg atagattcaa
    41461 tagaagggat gatcccttgc atatgaaatc cttcagtaaa gattttcccg aaataagtta
    41521 aacttccaac gatgatgctc gaaccggcac tcaacacttg aaatcctagg agtgtcttta
    41581 acgtgccaga gatgacttgt cctgctgatt tcttctgcag tagtagaccc aacatagcaa
    41641 tcaatgcaat ggtgatcgat gcctgagtca aaatattttc gatgataaaa ttgataacgc
    41701 ccatgtt
//
LOCUS       DANJMI010000024        40816 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000024 DANJMI010000000
VERSION     DANJMI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.024"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            23..646
                     /locus_tag="PWU81_001494"
     CDS             23..646
                     /locus_tag="PWU81_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110201.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="PWU81_001495"
     CDS             1096..2145
                     /locus_tag="PWU81_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1110202.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="PWU81_001496"
     CDS             2142..3023
                     /locus_tag="PWU81_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1110203.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="PWU81_001497"
     CDS             3026..3796
                     /locus_tag="PWU81_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1110204.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="PWU81_001498"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="PWU81_001498"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL1110205.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="PWU81_001499"
     CDS             5107..6270
                     /locus_tag="PWU81_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL1110206.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="PWU81_001500"
     CDS             6387..6797
                     /locus_tag="PWU81_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1110207.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="PWU81_001501"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="PWU81_001501"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL1110208.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="PWU81_001502"
     CDS             9370..9654
                     /locus_tag="PWU81_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110209.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="PWU81_001503"
     CDS             9814..10050
                     /locus_tag="PWU81_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL1110210.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="PWU81_001504"
     CDS             10047..10445
                     /locus_tag="PWU81_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL1110211.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11112..12056)
                     /locus_tag="PWU81_001505"
     CDS             complement(11112..12056)
                     /locus_tag="PWU81_001505"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL1110212.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12352..13296)
                     /locus_tag="PWU81_001506"
     CDS             complement(12352..13296)
                     /locus_tag="PWU81_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1110213.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLKIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13517..14731
                     /locus_tag="PWU81_001507"
     CDS             13517..14731
                     /locus_tag="PWU81_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1110214.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14753..16768
                     /locus_tag="PWU81_001508"
     CDS             14753..16768
                     /locus_tag="PWU81_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HDL1110215.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16851..18224)
                     /locus_tag="PWU81_001509"
     CDS             complement(16851..18224)
                     /locus_tag="PWU81_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL1110216.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18250..18534)
                     /locus_tag="PWU81_001510"
     CDS             complement(18250..18534)
                     /locus_tag="PWU81_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1110217.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18536..20551)
                     /locus_tag="PWU81_001511"
     CDS             complement(18536..20551)
                     /locus_tag="PWU81_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL1110218.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20730..21035)
                     /locus_tag="PWU81_001512"
     CDS             complement(20730..21035)
                     /locus_tag="PWU81_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HDL1110219.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21124..22155)
                     /locus_tag="PWU81_001513"
     CDS             complement(21124..22155)
                     /locus_tag="PWU81_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL1110220.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22362..23237
                     /locus_tag="PWU81_001514"
     CDS             22362..23237
                     /locus_tag="PWU81_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL1110221.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23389..24231)
                     /locus_tag="PWU81_001515"
     CDS             complement(23389..24231)
                     /locus_tag="PWU81_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL1110222.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24323..26200)
                     /locus_tag="PWU81_001516"
     CDS             complement(24323..26200)
                     /locus_tag="PWU81_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL1110223.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26197..27618)
                     /locus_tag="PWU81_001517"
     CDS             complement(26197..27618)
                     /locus_tag="PWU81_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL1110224.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27621..31010)
                     /locus_tag="PWU81_001518"
     CDS             complement(27621..31010)
                     /locus_tag="PWU81_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL1110225.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31267..32658
                     /locus_tag="PWU81_001519"
     CDS             31267..32658
                     /locus_tag="PWU81_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1110226.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32731..33378)
                     /locus_tag="PWU81_001520"
     CDS             complement(32731..33378)
                     /locus_tag="PWU81_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL1110227.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33386..34072)
                     /locus_tag="PWU81_001521"
     CDS             complement(33386..34072)
                     /locus_tag="PWU81_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1110228.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34078..35151)
                     /locus_tag="PWU81_001522"
     CDS             complement(34078..35151)
                     /locus_tag="PWU81_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1110229.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35373..36047
                     /locus_tag="PWU81_001523"
     CDS             35373..36047
                     /locus_tag="PWU81_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1110230.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36052..37392
                     /locus_tag="PWU81_001524"
     CDS             36052..37392
                     /locus_tag="PWU81_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL1110231.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37541..38527)
                     /locus_tag="PWU81_001525"
     CDS             complement(37541..38527)
                     /locus_tag="PWU81_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HDL1110232.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            39251..40282
                     /locus_tag="PWU81_001526"
     CDS             39251..40282
                     /locus_tag="PWU81_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1110233.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            40391..>40816
                     /locus_tag="PWU81_001527"
     CDS             40391..>40816
                     /locus_tag="PWU81_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDL1110234.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT        12670 a         7414 c         7661 g        13071 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc
    10741 catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg
    10801 tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt
    10861 agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca
    10921 ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga
    10981 tacattttcg gaccgattaa ttttaaccat tctatttatt tgcttaccta ttagatatca
    11041 ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt ttagttcata
    11101 ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt tgatttgctg
    11161 aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac accattttcg
    11221 tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc agttaaataa
    11281 gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat acgtgcagta
    11341 ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata agcagcatta
    11401 cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc tttttctaca
    11461 atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac ttctgaatca
    11521 ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg
    11581 atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac acgtgatgtt
    11641 ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt tgaagcaaca
    11701 acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg catgattgac
    11761 gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat accagctgtg
    11821 ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac agccacattt
    11881 tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc tttttcttga
    11941 ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc ataagcgata
    12001 cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt tttcatgaat
    12061 aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct tgtttattca
    12121 ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa acgaattcct
    12181 gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat aatacaacca
    12241 aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa taagacagca
    12301 atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt cttaatgttt
    12361 atttctccat tcattgggag tacatgttga atacttcttg aataatttga ataaagaagt
    12421 cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg tttcttgaat
    12481 cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg aagcatcaaa
    12541 atacattttc aatttattac taagatagac cggattgtag ccaaaatgag ctgctgtttc
    12601 ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa gcatctcatt
    12661 ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag tcaattcgat
    12721 cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg tatcaaaata
    12781 aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg
    12841 aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag aagaaagtag
    12901 attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt cttttaataa
    12961 gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag cttctttatc
    13021 aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa tttttaaaca
    13081 tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc
    13141 atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc ctcggtggat
    13201 ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca ttccttgttc
    13261 acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt
    13321 ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca cgctgtactg
    13381 ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact
    13441 tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga taagcgctta
    13501 caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt
    13561 ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac
    13621 ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct cttttatggt
    13681 cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt
    13741 gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct cacaaagtta
    13801 cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt
    13861 ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt
    13921 tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag gacagctagt
    13981 acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta ttcttgtctt
    14041 gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg gcacagcccc
    14101 aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct
    14161 ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc
    14221 tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc
    14281 tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc
    14341 tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat
    14401 tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa
    14461 ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa tcttattggt
    14521 tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct cttttgcttc
    14581 tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt tgattagcgc
    14641 gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa gagtagatga
    14701 agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta aaatgacaaa
    14761 tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc gccatggact
    14821 gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact ggtacatgga
    14881 tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac cttccatgtt
    14941 taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac atccatgtgg
    15001 aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag gacatacatc
    15061 agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg gctggacagc
    15121 aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg atatcgcaaa
    15181 agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg aaacggagat
    15241 tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag aactaatatc
    15301 tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg tagaagctat
    15361 ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga aagctcgtaa
    15421 acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc aacttcaagt
    15481 ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt cttctttact
    15541 gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt tgttttttat
    15601 cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat cattcatgat
    15661 caatcaatat cttttttttg acgaggaaat caataaacga acgatattag cattaagagg
    15721 acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct tgtggttgat
    15781 tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca tcgtctaccc
    15841 taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag ggtttattta
    15901 tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag aaggaacgct
    15961 tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt gcggagatat
    16021 cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc aagaaaaata
    16081 tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg gcgcttttat
    16141 cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt
    16201 attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct tattgaatga
    16261 cactatcaca cagattacta ctccatattt caaattctat gaacaagatg ctttatgcaa
    16321 actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag ggatgttatc
    16381 tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa atgaaaagta
    16441 cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag caagcccaat
    16501 ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt atgaagaatt
    16561 tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac ctatcaaaga
    16621 tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg atagaacagg
    16681 cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt
    16741 aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat atcagaagac
    16801 tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc ttaattctgt
    16861 tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa tacgacaagt
    16921 aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc tagtttccca
    16981 agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc gattttaaga
    17041 tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct attgaaagca
    17101 gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc agaagcagct
    17161 ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa gcctatcata
    17221 gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa tacagcatta
    17281 ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg tacgagtttt
    17341 ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac cccttgaagg
    17401 acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat tacagcgttg
    17461 tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca
    17521 tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa gctgaaactt
    17581 ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt tccgatccat
    17641 ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt gtttccagtg
    17701 atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt tccttcgaag
    17761 accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac taaaatgact
    17821 gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa tttatatctt
    17881 gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa agcagtaggt
    17941 gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac tactccttga
    18001 gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact gattaaacca
    18061 gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg gatcacttct
    18121 gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac gaggatggag
    18181 ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag ttcctccctt
    18241 gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt cttcgctgtc
    18301 aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg tatctttcaa
    18361 agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa catccgtatg
    18421 acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt tgatgttcat
    18481 ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt tcatatcatt
    18541 cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt aataaatctt
    18601 gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga agatgtgcct
    18661 cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga ttatcaagca
    18721 gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg ccatcttctg
    18781 gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca tactgctcca
    18841 cgcgctcgat catagctttg atataacttt cttctatttt attttctgat aatagcggct
    18901 gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat tggatcattt
    18961 cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct aaaagtttgt
    19021 tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct tttaattcga
    19081 tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat cctggtatca
    19141 gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc atgattggat
    19201 gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta gaaaaaataa
    19261 tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa
    19321 agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag ctaatgccac
    19381 ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc gcttttctga
    19441 tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg ctcttcccta
    19501 ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa gcatattgat
    19561 gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata cgaaaatagg
    19621 cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca aattctacgg
    19681 ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg
    19741 caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa aatataatct
    19801 ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta atgtcagtga
    19861 ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt tttccaattc
    19921 gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt gcgaatcgaa
    19981 gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa taatcaatgg
    20041 aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc atcaaacgag
    20101 cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc ctaaaaccag
    20161 ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt atctttttga
    20221 tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa
    20281 cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct tgttcggtat
    20341 acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt gctcggaatt
    20401 tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat tgtttgatcg
    20461 cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt tctcttggtg
    20521 ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac ttatattcta
    20581 gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg atttttttta
    20641 cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc
    20701 tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt ttttgaggtc
    20761 agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt ttcaaagtga
    20821 ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa agctgtttct
    20881 ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc ttctgtttca
    20941 ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat tgaacctctt
    21001 aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag tagttctata
    21061 ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat tcttacaaat
    21121 atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca aaattttcat
    21181 ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta atgtctcgta
    21241 aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt cttcatgata
    21301 actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat cgataccaaa
    21361 gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat cttttgtttg
    21421 tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa
    21481 ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat cgaagtaatc
    21541 attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat ctggttcgcc
    21601 gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat agctgttcgc
    21661 agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat aatgcatttc
    21721 tacttctaaa atatccccta attttccgct ttcgattact ttttggactg tcaagaagtc
    21781 agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt ttgctaatgc
    21841 aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta gtacattctt
    21901 accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg tagtgatgac
    21961 aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat attcgatgtc
    22021 ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat tgattttgtt
    22081 cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat tgccaagata
    22141 agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata tcctagaaaa
    22201 gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac acttgttatc
    22261 attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga
    22321 accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt gaagaaaaac
    22381 taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac atccgtagga
    22441 atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg tatacttccc
    22501 attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt
    22561 tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg attgatccta
    22621 attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg gcggatttat
    22681 cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta gatcaaatcg
    22741 gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat tctcaaatca
    22801 cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc atggctgaag
    22861 aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc gccattttta
    22921 ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg aagcatgtag
    22981 aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa caggctgata
    23041 tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact ttctcctctc
    23101 aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct caaaattttg
    23161 aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag
    23221 aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag tgtttgatag
    23281 gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt
    23341 ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct aacttctgtt
    23401 tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca gtatacatgc
    23461 agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta cttctttttg
    23521 aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat gactgttgac
    23581 catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga ttctcagatc
    23641 ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc
    23701 attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg tgaacaactt
    23761 tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc cccccgtagg
    23821 ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat tcgtttcatc
    23881 aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc
    23941 aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat ctgcacctgg
    24001 atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt ggattttagc
    24061 catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga tctgctgatc
    24121 cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta ttcctgagag
    24181 aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca tttgtgttct
    24241 ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct
    24301 ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca aagtacagtc
    24361 cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc ttaccgcctg
    24421 ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc aactgtcctg
    24481 ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat ggaacatatt
    24541 tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct gtcacatcga
    24601 ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat acccagctga
    24661 cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct tgatcacgaa
    24721 cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct actttaacaa
    24781 atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt gtatgcccgt
    24841 tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg ttcaaatgca
    24901 tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa ttcggatcaa
    24961 ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg tcgatttctt
    25021 tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca aatgttaaag
    25081 cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt ccttctttat
    25141 cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca ccgattgcga
    25201 catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca tttttgatgg
    25261 tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt gtttgctttt
    25321 caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt ccaacttcta
    25381 aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca ttgttgccga
    25441 tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt gtccaagtgt
    25501 acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct tcttctttag
    25561 aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt tttgtaactt
    25621 tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta tctagttctt
    25681 ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca gggaaagcta
    25741 aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc gtgtagactg
    25801 catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca gcatctgtcg
    25861 ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt gctgcatcca
    25921 cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa actgaaaatg
    25981 aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg ctgttttgga
    26041 ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga gctgctgtct
    26101 cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa
    26161 ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc ctgttctttt
    26221 tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc ctaaaagtgt
    26281 atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt tattgactac
    26341 tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg taaacggaat
    26401 ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc gataacttcc
    26461 tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc
    26521 tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt
    26581 gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg tctcgatttt
    26641 gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac caaaaccaaa
    26701 atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc ttagctgcat
    26761 ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga tcgaaaataa
    26821 aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct tttctccatt
    26881 cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac tatctgacaa
    26941 ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg aataagtgat
    27001 tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa aaggtggatc
    27061 agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt tttgattgtt
    27121 ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga cgacttctgg
    27181 tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt tagcggccag
    27241 agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa
    27301 cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta ccgttttgcc
    27361 cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg tgacaccatt
    27421 taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat gatttttcgg
    27481 cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt
    27541 ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac acatactaag
    27601 ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa aagataccaa
    27661 atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc aattcctccc
    27721 gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg gttgtttttg
    27781 gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga cccatcttcg
    27841 tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt aaaagtttcg
    27901 gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca
    27961 tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc tccttttaat
    28021 gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag tcgattttga
    28081 tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta aatgattttt
    28141 ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc ggcaggacca
    28201 gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc aagacctttt
    28261 tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt tttctgtagg
    28321 gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc atacttacgt
    28381 actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt atcatcggaa
    28441 gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt gatttcccaa
    28501 gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag cgtataggtc
    28561 atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc atgatctgtt
    28621 agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag atccttttca
    28681 cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt agtatttttc
    28741 catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc tttgattgtt
    28801 tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat ctcttgataa
    28861 ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt atttgttgta
    28921 tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc atttgtcgta
    28981 tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt gttgtcaaat
    29041 tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga tggtataccg
    29101 aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc gttcatttga
    29161 taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg gtaatggatt
    29221 tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt tccttctatg
    29281 gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga aagtgtacca
    29341 ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct tacagttgct
    29401 gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg atcagctgat
    29461 tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg agactctact
    29521 tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag accatgtatt
    29581 tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg
    29641 attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat tagagaagta
    29701 tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct gcttgattgt
    29761 gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag caaaacgatc
    29821 acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag tgctttttgt
    29881 gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa
    29941 tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct tgagtagccg
    30001 acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc gacaaaacga
    30061 tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc tgaccagtct
    30121 acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg agcattcaaa
    30181 taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat cgcatagtct
    30241 gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt aggatctcca
    30301 ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg atgatcaaga
    30361 acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg atcatctgtt
    30421 gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa ggataatgta
    30481 ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc ttctatttcg
    30541 tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc aataatgtca
    30601 tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt
    30661 ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt aattttcccg
    30721 ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact taagtaattt
    30781 tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc atttagcttg
    30841 atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat tgtttctaaa
    30901 actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt tgataaactc
    30961 aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat ttttcccctc
    31021 ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa acaggcgtta
    31081 cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa gggaatcaca
    31141 ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta atgagcgttt
    31201 cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga aggaaactat
    31261 agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat gactaaaaaa
    31321 gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat cgaagagatc
    31381 aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg gtatcgtctt
    31441 gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca aagttctaca
    31501 aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga gtattattgt
    31561 gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg caatcaaatt
    31621 ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac tagccttaaa
    31681 agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta cggaagacgc
    31741 acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa gaataattgt
    31801 cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat tatgtatttt
    31861 gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc tggagactct
    31921 tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa tctcttagga
    31981 aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac gattttttca
    32041 gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa tatgtcctac
    32101 tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt tcaattatta
    32161 acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt gacgatccgt
    32221 gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc tggatttcgt
    32281 gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta tttatctagc
    32341 tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc tgataaaaaa
    32401 atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga atatatcagt
    32461 gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga agatcagctg
    32521 tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat tttgaatgcc
    32581 catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga agaactcatc
    32641 cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc tgataaaaat
    32701 ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa tccaacgaga
    32761 tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa tgagatccgt
    32821 caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt cttccgacga
    32881 ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca ttggagaatc
    32941 agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta acaagtataa
    33001 cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat atttttcgat
    33061 ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt gatggactat
    33121 gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa aatatttata
    33181 aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga cttggctgat
    33241 gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct ttgcgttcga
    33301 taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga ttatacgtgg
    33361 atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct gtcaagtacc
    33421 catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca tgagtaacca
    33481 taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg acttcatatg
    33541 catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt ggctcattat
    33601 ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct ttaggataac
    33661 tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt gttgaatctt
    33721 ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt aaaaaaggaa
    33781 tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct gctcgttttt
    33841 tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa gtcggtgttt
    33901 gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga ccaatgatgc
    33961 tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag acctttgtat
    34021 gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc atttttatta
    34081 acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa acaatccgcc
    34141 tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc aaaaaacagc
    34201 aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa gagcaagcag
    34261 aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga tgaagaaatt
    34321 tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg tcatcacgta
    34381 catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca tcgcatttaa
    34441 ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa tagttaataa
    34501 ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta ttacgatccc
    34561 attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca aagcagtcca
    34621 cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag gaaagatacc
    34681 gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa ttttatagcc
    34741 tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt catgcccttt
    34801 cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa cattttcttt
    34861 gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc ctccttcaat
    34921 gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg catcctccga
    34981 caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt ctgaaagtcc
    35041 attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa taccgacgat
    35101 caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca tttgtttttc
    35161 ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta actctaaagt
    35221 gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga catctttcga
    35281 taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct ttccatgata
    35341 aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta gtagaagacg
    35401 ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag ataacacaag
    35461 tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat cttgcaatct
    35521 tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata cggaaagaaa
    35581 aaatacagac tccagtattg attttgactg caaaagatag tttggaagat aagattgaag
    35641 gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa gaactgatca
    35701 tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat gttctgaagt
    35761 ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa caagttttgc
    35821 ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag ggaatcattt
    35881 tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg acgattacag
    35941 ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga ttggataaag
    36001 aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc aatgaatgga
    36061 gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc atttgctttt
    36121 atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc atacagagaa
    36181 acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt agagatacaa
    36241 agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa cagaacaaac
    36301 atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt gatcttgtgg
    36361 tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa ccaaatcaaa
    36421 gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt aaatgaagac
    36481 agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac gaatcaagat
    36541 ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc attagcgact
    36601 tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat tggtatcagt
    36661 tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag acaaaaagaa
    36721 tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca aaatagtttg
    36781 gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag catcgctcaa
    36841 gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac aatcgctcgg
    36901 aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga atatatcgaa
    36961 caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt tattctccat
    37021 aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt tttggttatt
    37081 ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt ggattcatct
    37141 gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat cagtgaagaa
    37201 gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc gaaagagact
    37261 ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca tcatggacag
    37321 atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt gccgttgaag
    37381 cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt cacagccctt
    37441 ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttct
    37501 ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt ttattgcggt
    37561 cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta acatcttctg
    37621 tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca cgaataactg
    37681 tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg tcatcttcca
    37741 atttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg atgacgattt
    37801 cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt ttgtggatgt
    37861 cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct tttagttgaa
    37921 ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt gtacccattg
    37981 tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc aatggatccg
    38041 ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca gtgccagttt
    38101 ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc attgtatctt
    38161 ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca tcagaaatat
    38221 ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa tcataggtat
    38281 caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca ccatccatcc
    38341 gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt ctagatgctg
    38401 cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta cgcaaccgtt
    38461 caattactga taatgcttct tccctcgtac tatagcttcc gatgaccgtt aaatttttct
    38521 cttccatgta aattcctcct agcaaataga tttatgattg gtatattgtt acttataaat
    38581 ttattctata ggagacgatt gttttctaca aatatttaga ttggaatttg attaaaacaa
    38641 tttaattgaa atttgaaaac atttatgtta atgaaagtca tattctaata aaatgtcatt
    38701 tgtttctata tgaattttta tgaaaaattt catataattt ccactcgttg tgatggttta
    38761 atttatccaa tccatcaata agcaaacaac ttatataaaa aaggattaat tctcaatata
    38821 attgctccta ccatagaata aatcttcatg taaagtaatg aagtaccaac tatttctaat
    38881 acttctttaa gaattatccc aacaatatcg ttagacaaac cattattgaa taaatggaga
    38941 gccaatcgat tctgaaaaaa tgttttctaa tttttcggaa ataaaagaaa gagcctcctg
    39001 ccattagaac gaaccaaatc catttcattg ctcatgaagt aaccacatag ctaaaaagag
    39061 gataaatcaa ttaaaagatc aaaagaatga ctgcactaat aaaataacta aaaatgtatc
    39121 ttcttacttc atatctttta gctagtactt agttaaaaat ttgcttccta agatcaagac
    39181 aaactgtcta tcttcatttg atgaaatgcg gtaaaatata gaaaatgaac actattggag
    39241 ggaatttgta atggacagca aaactagaaa acaactgcaa gaaaagttaa agcaacgtac
    39301 acacttgctc ccttccgtct ttcaacagtt tttagaagaa aatcgttctt ccctttcatt
    39361 aggcagtcgc tatgaatacg caaaagattt tcatctgttt actgattatt tgagaaaagt
    39421 attttctgaa gaagtggatg atcagctgat tatccattta gaaaaacaag attatcagaa
    39481 tttcttagct aaaatctatc gacatactcg ttcgttccaa actacagcga accaagaaac
    39541 ggagcaattg tttgaaaata gctattacgg gatttcaagg aagcaatatt cattaaatca
    39601 ttttttacga tttttgtatc aaaaaggact gattgaagaa gagatttctc ttttgggtac
    39661 aagtttaccg gaaacaacca aagcagctcc gcgatatatc gatgcagaac ttttcaaaga
    39721 ttttgagcat ttgtttatcc cgattgaagg ctttcaaaca agcagagaag ctagctttga
    39781 agaaaagaat agaccgagga accttgcaat tactgccatt cttctgtatt gcggattaaa
    39841 gatccatgaa gtagtagaac ttaaacgtaa agatgtatct ttgaccaagc agattcttaa
    39901 tctgaataga aaagaaaaca gactgaataa agtaccaata ccaaaagaag cgatgccatt
    39961 tctggagaac tattttaagt taacagcaca aaatacggac gaaaattctt ttgtttttcg
    40021 ttcctcacat gatcagcaaa tttctcctcg gactattcgc caaattttga gcaaaattac
    40081 agtatataaa aacgtatcgc cagaactatg cagaaaaaca tatcgttatt atgcagaact
    40141 atatttagat gatgctgacg cggtaaatta tttatgtggt aatcgatacc tctcatccca
    40201 ttcttttcaa gatatctggg aaaagatggt tgatttttct tatcacgaat tagcggcatt
    40261 cgttcgggtt tcttccatat aaaacaaatg tatgatcttt ttttcacaat aaactatgtt
    40321 attatataaa ggattatgtt acgattatct tgaatttgta tattacttaa caaaagataa
    40381 tggagtgtga atgatggaga ccataacgta ttcccctttg aacttattta cgaattttca
    40441 agaagctgca gctaactttt ccgagacccc gattatcttt gatgagccac tttcagcttt
    40501 cccagaatta ggacttgaaa caacttacaa aaaatgctgc gaaagtgtgt tgaaacgggc
    40561 ttatcaattg gctcatcttg gagtgaaatc cggagacaaa gttattattt ataagagttc
    40621 cgcttttgac acataccttc tagctgtttc tgtgtcttac cttggtgcag tgcctgtcat
    40681 gacttcttat catctaccaa caacaacaat ggaagtattt atcgatcgtt tagaagaccc
    40741 tttcatttta tttgatgatg agacaaaaaa gcgagtgaga gaaatttcaa atggaacaaa
    40801 atcaaaacag attcct
//
LOCUS       DANJMI010000025        40372 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000025 DANJMI010000000
VERSION     DANJMI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.025"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="PWU81_001528"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="PWU81_001528"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL1110235.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="PWU81_001529"
     CDS             complement(2262..2513)
                     /locus_tag="PWU81_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HDL1110236.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="PWU81_001530"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="PWU81_001530"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDL1110237.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="PWU81_001531"
     CDS             3463..3681
                     /locus_tag="PWU81_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110238.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3970..4482)
                     /locus_tag="PWU81_001532"
     CDS             complement(3970..4482)
                     /locus_tag="PWU81_001532"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL1110239.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="PWU81_001533"
     CDS             complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="PWU81_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL1110240.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6935..7378)
                     /locus_tag="PWU81_001534"
     CDS             complement(6935..7378)
                     /locus_tag="PWU81_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL1110241.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7379..8167)
                     /locus_tag="PWU81_001535"
     CDS             complement(7379..8167)
                     /locus_tag="PWU81_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1110242.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8164..9105)
                     /locus_tag="PWU81_001536"
     CDS             complement(8164..9105)
                     /locus_tag="PWU81_001536"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDL1110243.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9282..9869)
                     /locus_tag="PWU81_001537"
     CDS             complement(9282..9869)
                     /locus_tag="PWU81_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HDL1110244.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10090..11397)
                     /gene="obgE"
                     /locus_tag="PWU81_001538"
     CDS             complement(10090..11397)
                     /gene="obgE"
                     /locus_tag="PWU81_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDL1110245.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11691..12701
                     /gene="gap"
                     /locus_tag="PWU81_001539"
     CDS             11691..12701
                     /gene="gap"
                     /locus_tag="PWU81_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL1110246.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12786..13721)
                     /locus_tag="PWU81_001540"
     CDS             complement(12786..13721)
                     /locus_tag="PWU81_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL1110247.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13907..14866
                     /locus_tag="PWU81_001541"
     CDS             13907..14866
                     /locus_tag="PWU81_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HDL1110248.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(14952..16046)
                     /locus_tag="PWU81_001542"
     CDS             complement(14952..16046)
                     /locus_tag="PWU81_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL1110249.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            16235..16996
                     /locus_tag="PWU81_001543"
     CDS             16235..16996
                     /locus_tag="PWU81_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL1110250.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(17111..17551)
                     /gene="fabZ"
                     /locus_tag="PWU81_001544"
     CDS             complement(17111..17551)
                     /gene="fabZ"
                     /locus_tag="PWU81_001544"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL1110251.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            17771..18526
                     /gene="fabI"
                     /locus_tag="PWU81_001545"
     CDS             17771..18526
                     /gene="fabI"
                     /locus_tag="PWU81_001545"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDL1110252.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(18636..19649)
                     /locus_tag="PWU81_001546"
     CDS             complement(18636..19649)
                     /locus_tag="PWU81_001546"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL1110253.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            19814..20734
                     /locus_tag="PWU81_001547"
     CDS             19814..20734
                     /locus_tag="PWU81_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDL1110254.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            complement(21014..21514)
                     /locus_tag="PWU81_001548"
     CDS             complement(21014..21514)
                     /locus_tag="PWU81_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL1110255.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(21621..22139)
                     /locus_tag="PWU81_001549"
     CDS             complement(21621..22139)
                     /locus_tag="PWU81_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL1110256.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            complement(22140..23495)
                     /gene="rph"
                     /locus_tag="PWU81_001550"
     CDS             complement(22140..23495)
                     /gene="rph"
                     /locus_tag="PWU81_001550"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL1110257.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            complement(23498..24319)
                     /gene="racE"
                     /locus_tag="PWU81_001551"
     CDS             complement(23498..24319)
                     /gene="racE"
                     /locus_tag="PWU81_001551"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL1110258.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            complement(24481..25137)
                     /locus_tag="PWU81_001552"
     CDS             complement(24481..25137)
                     /locus_tag="PWU81_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL1110259.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            complement(25153..25800)
                     /locus_tag="PWU81_001553"
     CDS             complement(25153..25800)
                     /locus_tag="PWU81_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL1110260.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            complement(25813..26628)
                     /locus_tag="PWU81_001554"
     CDS             complement(25813..26628)
                     /locus_tag="PWU81_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL1110261.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            complement(26642..27379)
                     /locus_tag="PWU81_001555"
     CDS             complement(26642..27379)
                     /locus_tag="PWU81_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL1110262.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            27633..28643
                     /gene="trpS"
                     /locus_tag="PWU81_001556"
     CDS             27633..28643
                     /gene="trpS"
                     /locus_tag="PWU81_001556"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL1110263.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            complement(28813..31233)
                     /locus_tag="PWU81_001557"
     CDS             complement(28813..31233)
                     /locus_tag="PWU81_001557"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL1110264.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(31238..32281)
                     /gene="pheS"
                     /locus_tag="PWU81_001558"
     CDS             complement(31238..32281)
                     /gene="pheS"
                     /locus_tag="PWU81_001558"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL1110265.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            complement(32590..32931)
                     /locus_tag="PWU81_001559"
     CDS             complement(32590..32931)
                     /locus_tag="PWU81_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1110266.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            complement(33000..36722)
                     /gene="addA"
                     /locus_tag="PWU81_001560"
     CDS             complement(33000..36722)
                     /gene="addA"
                     /locus_tag="PWU81_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDL1110267.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            complement(36719..40246)
                     /locus_tag="PWU81_001561"
     CDS             complement(36719..40246)
                     /locus_tag="PWU81_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDL1110268.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
BASE COUNT        11436 a         8670 c         7046 g        13220 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat
     3901 ctgacttttg agggtcgctt taaaatcgct tctttaagaa taacatcaag aatagcttct
     3961 tttttctgct taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc
     4021 atggagatcc atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc
     4081 gatcagttcg atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag
     4141 cacacgttga cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc
     4201 taatccataa gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc
     4261 aaaaccgact cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg
     4321 cccaactacc atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc
     4381 ccgataagcg tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga
     4441 cggatagtcg ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg
     4501 ttgttaacca tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga
     4561 ttttattgag ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag
     4621 cgactttttg taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta
     4681 tcataaaaat taataatttt tcagggatac gcaaatattg agctattgct tttattttat
     4741 gacgtatatc aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc
     4801 ttgtgcctac cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat
     4861 tgaatgaata agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa
     4921 caactagaga gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga
     4981 aaacaatcgc agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg
     5041 ctaaataaag gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat
     5101 caaatacttg cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc
     5161 cgttccattc attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt
     5221 cttcttgagt accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga
     5281 caaatttcag atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc
     5341 gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat
     5401 caattcgctc tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc
     5461 ctctcatcaa gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag
     5521 tgacattttc acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa
     5581 ataaatctcg catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag
     5641 atcctttagc tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat
     5701 tcattatttt cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata
     5761 gatggatatg attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga
     5821 ttgcttttct ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt
     5881 catcaaacgt cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat
     5941 ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc
     6001 cgctttcttc aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc
     6061 ctaatgaaac aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta
     6121 tcgctactaa atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga
     6181 atgctactcc gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt
     6241 gaacaatcgc ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca
     6301 cgtctacacc agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa
     6361 cagtgatgat caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc
     6421 catacccatg ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca
     6481 gtgtttcttt cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga
     6541 ggattttttc tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt
     6601 gtatcagata cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct
     6661 tttcctcatg gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt
     6721 cacggatcat ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat
     6781 ttgcttgtct cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa
     6841 aaagcagaaa gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct
     6901 gatcttttgt acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt
     6961 tgggcgattg agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg
     7021 cttgtaactc cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt
     7081 gctgttcttc ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt
     7141 cctgagtcag ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa
     7201 atacaatcag tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa
     7261 aggaagtgaa gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa
     7321 taatcaaaac taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct
     7381 actttttgtt aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa
     7441 attttgatgc aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat
     7501 tgcggacgat catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca
     7561 aatagcttcc gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca
     7621 ctgtcgtaac agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa
     7681 ggactgcaaa tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca
     7741 tagaggcgat attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt
     7801 gtgtcaaaac catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt
     7861 cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc
     7921 gcccgacttc ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt
     7981 ctaccttgaa agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt
     8041 ctacaaatgc cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat
     8101 aacagatttg tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta
     8161 aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat
     8221 cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa
     8281 atatctcgca ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc
     8341 agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt
     8401 aaatgtgctt tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt
     8461 ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc
     8521 ttttccattg atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc
     8581 gtacagtggt ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt
     8641 atgatcatgt tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt
     8701 ataaacagaa aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt
     8761 cagcggataa cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat
     8821 cccttttggc ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat
     8881 gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat
     8941 ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa
     9001 caaccaaact tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg
     9061 ttttgcagga acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt
     9121 ctttaagttc ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa
     9181 tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa
     9241 aaacagcagc gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa
     9301 ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct
     9361 ttcgcatgat tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc
     9421 aagcgagtaa tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc
     9481 tcttcatcag caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag
     9541 tcgaacacgg tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct
     9601 ccggcaaacc atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg
     9661 attaatccac ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg
     9721 aattgatgtg tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa
     9781 cgaacagcat gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga
     9841 aaatttaaaa tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat
     9901 ttattttacc tttaattgaa aaataagaaa agaaaagcac tctttttttc caataaaata
     9961 gggctgtgac aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct
    10021 gatcacttct gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta
    10081 tttgcttgcc tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc
    10141 tttcgcaccg cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa
    10201 acgcatcact gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc
    10261 accagataag atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg
    10321 attgaatcca tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg
    10381 cgttacttct aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac
    10441 tccagaaatt ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag
    10501 ctgttctttg aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac
    10561 gatgatttgc ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc
    10621 aagatagtct tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat
    10681 tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc
    10741 tccttcaatc aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac
    10801 cattcctaga ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga
    10861 tgaaataacg gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc
    10921 tgccaatact ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc
    10981 aatttccggt gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc
    11041 accttttgcc acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt
    11101 ctccgcatct cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg
    11161 accgtgcatc cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa
    11221 ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg
    11281 accaccgtct ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac
    11341 cattccgtct ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga
    11401 cataattaag tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa
    11461 caaaagtctt acttatttta tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga
    11521 ttgtttacct tcttcttttt ttatcatttg gtaaggtttc caaccttttt tctgctacca
    11581 agtataattt tttgacaaaa tattctttgc gtagcagaat aacagtgtat taattgtatt
    11641 aattgaggat gccgtttcac agcaccccta ataaaggagg aacttttgat atgacagtaa
    11701 aagtaggtat taacggattt gggcgtattg gccgtctggc tttccgccgc atcaaagaag
    11761 tctcagatga tattgaagta gtagcaatca acgacttaac aagtccaact atgttagcac
    11821 aattattgca atttgactct actcatggta cttatcctgg cacagttact gcaacagaag
    11881 actcaatcgt agtggatggc gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa
    11941 tcccttgggc aaaagaaaac ggtgtagata tcgtactaga atgtactggt ttttatacat
    12001 ctgaagaaaa agcaaaagcg catttagatg ctggtgtcaa acgagtagtt atttctgccc
    12061 cagctggtgc tatgaaaacc atcgtctata acgtcaatga tgatacatta gatgaaaacg
    12121 acaaaatcat ctcagcaggt tcatgtacaa caaactgttt agcacctatg gcttatttct
    12181 taaataatga atttgggatc gaagttggta caatgacaac agttcatgcc tacacttcca
    12241 cacaaatgct cttagatgga cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg
    12301 ctgacaatac tatcccacat tctactggtg ctgcgaaagc aatcggctta gttatccctg
    12361 agctacaagg aaaattacaa ggacacgcac aacgtgttcc agtcgttgat ggttcattaa
    12421 ctgaattggt ttcagtattg aaaacaaaag taactgctga tcaagtaaac gaagcaatca
    12481 aaaaacacac agttgacaat ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg
    12541 atgtcattgg aacaactcaa ggttcgatct ttgacccaac gcaaacagaa gtaacaactg
    12601 ctggtgattt ccaacttgtt aaaactgttg catggtatga caatgagtat ggatttactt
    12661 gccaaatgat tcgcttacta gaaaaattcg ctaacttgta atttgataga tcaatcgata
    12721 aaagaagcct ttgactggat ccagtcaaag gcttctttct tttcatcccc tacttttaaa
    12781 ctcgttcaat gaacatattg gcttaaaaat tgttggacta cttgctgata tttttcagga
    12841 tctgtcttgt atgcttccgc atgctcggct ccttttacaa catacttttc ttttggtccc
    12901 ttcgttgcac gatagacctt atctaacatt tcaaatggta caaatgtatc ttcgtctcca
    12961 tgtataaaga gcattgggcg ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg
    13021 aaaaagtagc cagcacggac ttttgtcact agactcgtta cttgcataag cggaaatgct
    13081 ggaagactaa acatatcgtc tagctgataa gcaagctcat catgaacgga agaataaccg
    13141 caatcttcaa tgattgcttt cacattatct ggtagttttt ctccgctagt catcataact
    13201 gttgctgctc ccatgctgac accgtataga acgatttctt gcgattttcc attgttttct
    13261 agcactttgt ttatccactg tacatagtct ttacgctctg gccatccaaa tccaatgtaa
    13321 tccccttcgc tttttccatg tcctcttgca tcaggaacaa ggacattgta acccatatca
    13381 tgaaacatct ttgcgtaatt ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca
    13441 agagctgttt tttcactttt cgtttcagca ggaagataaa tcgctgataa tttcagaccg
    13501 tcttttgaag tgatggtcca atcgcttcgg ttttcttttg aactaaacca tttatgtgca
    13561 tccgtttctt tggatgtctg tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca
    13621 gaaggcacaa cagcataatt ataaaaataa ttccctgcaa acactaacgc aacaatcaga
    13681 gctaaagcga cgcctaaaag gattttcatc caaagtttca taaatcatcc atctcatttc
    13741 tcttttttca tataaccttg aattagtata atcaaagaat ctctactatg caatgttcat
    13801 ttttataacc cgcgaattgt aaaattaagt tagaaaaata aaaaggcatt tatggtacac
    13861 tcaaattgta tttccgacga aagaaaacaa aggagtgtaa ccataaatga cctacaaaca
    13921 tcttaccata gacgaactga caatgataga atcatattat cttcaacata ataagccggt
    13981 tgaaatcgct aaccgaatgg gacgtgctat acaaactatt tataatgtag tcaataagtt
    14041 caagcaaggc aagactgctc ttgattattg gcaccagtat aaagaaaata agaaaaaatg
    14101 tggtagaaaa gtcattcaat tacctgctca tgaagtagat tacattaaag agaaagtcac
    14161 tcttggttgg acgcctgacg tcattatcgg gcgaaaagaa aggtctgttt catgcggtat
    14221 gagaacactt tatcgtttat tttctaaagg aatatttgat attgacacac taccgatgaa
    14281 aggtaaaaga aaacccaatg gccatcagga aaaacgggga aaacaacagt atcagcgctc
    14341 aatccatgat cgacctgata attatcctga tttcaattct gagtttggtc accttgaagg
    14401 tgatacgatc gttggcattc atcataaaag tgccgtcatt actttagttg aaagattatc
    14461 taaagtcatt atcacgatta aacccaacgg ccgtaaggca ttagatattg aaactgccct
    14521 taatcaatgg ttttctcgct tccctaaaaa cttctttaaa tctattacgt ttgactgtgg
    14581 aaaagaattt tctaactgga aagccattag taaccaacat gatattgata tatattttgc
    14641 ggaccctgga acaccttctc aacgcccatt aaacgagaat tctaacggga ttctgcgtcg
    14701 taatggactg ccgaaatcaa tggattttag aaaagtgaat cagacattta tttccagtgt
    14761 cagcaatcaa cgtaatcata ttccaagaaa atcattgaat tacagaacac caatcgaaat
    14821 atttttgagc tatgtacaag aagcatttta ttctagctta atttgacaaa tcataaccta
    14881 taaaaaaaca aaataaaaaa gcagaaataa ctggctattt ctagcggatt atttctgcaa
    14941 taactaatta tctatttttt attttcaatt aatttaaagc ttgaccatgg tttcaaaaag
    15001 tcaaggagga ataattcgtc atcggagatt cttcctacaa cattgacacg tccgtcgttt
    15061 ttcatatcct tcaaggcgat ttgtgtttct cctttgtatt gaccgtaccc ttcattgtca
    15121 atcagtacat ccccatgatg catatctcgc gtattatgag gcgggaaatc tttatctttg
    15181 tagtatacac gagtcatcgt tgaacgaagg atatattctg agcgatcccc tcgataacta
    15241 tgaaggttat caaacaagca gattcgttcg ttttctgtaa taccttcttc cgtatctact
    15301 ttcaatgttg gataatccgc gttaaatgct tctgccattt ctttcaattc ttcttcactt
    15361 gcgtacgcat ttccaataga aatatcatca atcaatcctg taaggattaa atgcttcact
    15421 tgtgtcgcaa tttcaagatc tcggtggtct tccagcgtgc acaagccatc ttgcgtaggc
    15481 caagggccaa actcagcaga ttgcgaattg acgaatgcca tcgtattcaa attatatttt
    15541 ctgaattttt ctgaacaaaa aacaaagtgt tcgtatccta aaccagagta tcgatgagga
    15601 taaaaattat gagaacctaa taaattatcc gtgtttggcg aataactcat gatgttgtca
    15661 acgtaattcg tgcccgaact catattgatc tcgattttga taccaaaagg attgcgcgtc
    15721 atttttgctt cttctgctcc tgtgaatccg atatctaagc ggaccccgta ggcacccatc
    15781 ttatggaaaa aagataagtc atcgtaagaa atctccaact gttcaaataa cgctggattg
    15841 atatcaacca ttacttccat gcctaggcta ttcgcgtaat caactacttc tttaaattct
    15901 gaaagaactt tttctttatc atcattgatc tgcagcaaac tggtaaaaac ccgtttatac
    15961 ccatacttat gagctaaatc caagtacgct ttgtcctttt caaatgttga ccgttctgga
    16021 taaatcgaga ttcctaattt tcccatatta ccatttcctc ctgcatcttt tcgtttttgt
    16081 atccacttac attatagcaa acacaaagta aatggtctac accttttctt tttcttttaa
    16141 aataaatgat aaattccaat aatttaagta ggtattttcc ctctattttg ttatcattaa
    16201 agataacaaa atagtaaatt ggaaaggaac agttatgaag aaaaataaag ttattttaag
    16261 tattagttta gcagcagcat tgctgtttgc tgctggttgt tctgcaaaca acacgacttc
    16321 ttctagttcg tctacagaaa gttcaaaaag tacatctact gcggaaagca cgactgccag
    16381 tagcgtcgat ttgaacgcat tggaattacc acaattgacg aaagatgtcg ccgaaaatga
    16441 agatctggtc gaaatggtga cgaataaagg cacaatcaaa atcaaattat ttcccgaaca
    16501 cgcaccaaaa gcggttgaaa actttatgac acatgcaaaa gacggttact acaacggact
    16561 gactttccat cgcgtcatca aagatttcat gatccaaggt ggcgatccta aaggcgatgg
    16621 cactggaggg gaaagcattt ggggagaagg atttgaaaca gagatcaaca accaccttta
    16681 taatataaga ggtgcgttat ctatggctcg ctcacaagat cctaacagta acggcagcca
    16741 gttctttatc gttcaaaata cagatgatat gcatgatggt cttttaaaag acgactatcc
    16801 tcaagcaatc atcgacgctt ataaaaacgg cggttatcct tcactagatg gccattatac
    16861 tgtcttcggc caagtgattg aaggaatgga tgtcgtggat gctattgcaa atatcgaaac
    16921 agatagttca gacaaaccaa aagaagaagt aaaaatcgat aaaatcaata tactgcaaga
    16981 agcaaaacaa aaataaaaaa ataacgtctg aatcctgcat agatgacaac gtatgctagg
    17041 attcaggcgt cttcttttta cataaaaagc aagtaagctt ccttttgttt tgctatatca
    17101 ggttttatag tcaagcattt tggttgacga tgaacgtgaa ctcgcactca cagacttttt
    17161 tatcatctac atatgctgca gataacgctg taccgactga tcccttcatt ttggtgattt
    17221 caaaatgaca tttcagcaca tctcctggaa caactttttg acggaatttg gccttattga
    17281 tcccaccgat ataagctctt tttcctagaa actcttctga ttttaggata agtatcgaac
    17341 ctgcttgtgc cagtgtttct agaatcaacg cacccggcat cactggattg tcaggaaagt
    17401 gaccttggaa aaaatcttca ttgattgtta cattttttgt cgcaacgatc ttttttcctg
    17461 gttctaaagt atctacataa tcaatataac aaataggata acgattagga atcaattcca
    17521 ttacttcagt agcagataat acgtaagaca taattaaact ccttctttca ggcaattgcc
    17581 attttcatta ctttgactat caaagtatca tattcctaaa tatttagcaa tcttttttta
    17641 atttaagaaa aaatagtttg acaatcaaaa cattttgact aataattaag aaatcaagaa
    17701 gttattttag ctattattct ctattttttt acgactatca aatttttatc agaaacaaag
    17761 gagagtcatt atgtcatttc tagaaggaaa aaaaatcgtc attatgggcg tcgctaacaa
    17821 aaaaagcatt gcttggggtt gtgctgaagc aatgttgaaa caaggggcag aaattatcta
    17881 tacttaccaa aatgaacgaa tgaaaaaatc attagtcaaa ttggtcggcg aagaagcatt
    17941 cactgtagaa tgtgatgttt ctagcgacga aagcatcgaa aaagcaatga atgccatcgg
    18001 tgaatatgct ggggagatcc acggccttgt ccatgcagtt gcttacgcca acaaagaaga
    18061 attgtccgga aatgtgtctg atatttccag agacggctat caattagctc aagatatcag
    18121 cagctactct ttaattgctg tatcaaaata cgcacaaccg tttcttgcac caaacagcgg
    18181 gatcgttagc atgacttatc taggttcaga acgcgcaatc ccgaattata acatgatggg
    18241 tattgcgaaa gcttctcttg aagctgctat ccgttatttg gcagctgagt tctcgcctaa
    18301 aggaattcgg gtcaatggaa tctcagctgg ggcaatcaaa acattagctg tcacaggtgt
    18361 taaagactat caaaaactga tccagttatc tgaatctcgt acgcctgatc agcaaggtgt
    18421 gacgatcgaa gaagtcggta atgcttgtgc tttcttaatc agcccacttt ccagcggaat
    18481 catcggtgat attatctacg ttgacaaagg tgtccatctt tcataaactt ttgttgcatt
    18541 ctttaaagta aaacatataa aaatcaggaa aaaacttttt aaagtctctt cctgattttt
    18601 tctttttacg tttttttcaa ctcattactt tgaaattatt ccacgacttc aatccagcct
    18661 tctggtgctt ctatgtctcc aaattgaata cctgtcaatt catcatacaa ccgttttgtt
    18721 actggtccta cttccgtttc actataaaat acatgaagat tgttattgtg ataaatccca
    18781 ccgattggag aaataacagc cgctgtacca catgcaccag cttctgcaaa ttgatctaga
    18841 tcatcaatga aaatatctcc ttcaattgct tgcagtccta gtcgttcttc agctaactgc
    18901 aacaaggaat atttcgtgat acttggtaag atagagggcg attgcggagt gatgaatttt
    18961 ccgtcttttg taattccaaa gaaatttgct gcacctactt cttcgatttt cgtatgtgtg
    19021 gctggatcaa gataaacaca atctgagtaa tccagttctt ttgcttctgt tcctggaagt
    19081 aaactcgctg cgtaatttcc gccgactttt gctgcaccag tccccttagg agcagcacga
    19141 tcgtaatcag atacaataaa attcgttggt tttagtccac ctttgaaata cgctcctact
    19201 ggcgtacaga agacgacaaa taaatattct ggagctggat ggacaccaat gttttcaccg
    19261 accccaatca gtaaagggcg caagtataat gtagcgcctg ttccataggg cggcacaaaa
    19321 tcttcatttg cttttaccac ttgcttgaca gcatcaacga acatcttttc agggaccatt
    19381 ggcatcagca atcgtctagc actttgattc atccgtcgac tattttgttc aggtcgaaag
    19441 agattgacct tgccgtcttt tcgacgataa gcttttagac cttcaaaaca ttgctgacca
    19501 taatgaagcg ctggagagcc ttcatgaatc gtcaggtaat tatcttctgt caattctcct
    19561 tcttcccatt gtccgtcttt ccatttggca ataaaacgcc aaggagtttt gatataagaa
    19621 aaacctagat tgttccaatc gatttgcata tacttaccac cttcgttatt ttctccacat
    19681 tttatcatta tccatattat ttgtcatttt attttcagct tttttccttc attatagtta
    19741 tttttgctat ttcctttttt gaaaacttct tggtacaata cagaaagaat caaattttag
    19801 aaaagaggat tttatgtttt cattattagc aactgccggt acgacagact cttctggcga
    19861 gacattgaca gatcaagctg tcagcaaagt aaatgcgttt cagcggtttt gggacgggat
    19921 tgattgggac gccattttgg ctgcctttat ccaaaaaagt ttatatctga tttttttgat
    19981 cgtccttttt tggattcttc accgtgtggg aaaatatctg attgataagt cttttaatca
    20041 gtattctaaa aaagccattc taaacgagag ccgcttaaag actttacata gtcttttaaa
    20101 tactattttt cattataccc ttggtttctt ttttatctat gcaattttat ctgctatcgg
    20161 tgtgccgatc ggatcacttt tggctggtgc cggaattgca ggtgtggcaa tcggtcttgg
    20221 tgcccaaggg ttcatgagtg atgttattac cggatttttt attatcatgg aacaacagat
    20281 ggatgttggg gactacatca agctagcaaa cttaacgatt gaaggaaccg tcgtatctgt
    20341 tggtatccgt acccttcagc tgaaagcaac ggatggtact gtccatttca tcccgaatcg
    20401 aaacatcacc acgatcagca atctctctcg tgcaaatatg caagtacttt tagatattcg
    20461 gattgttcct gaagaaggat atgacaaaat ctatgagatc attgatcgag taaatcgtac
    20521 attagctgaa aaataccaag aagagctcca aactgaacct tctatctttg gattagtgga
    20581 tcttggaaac agcaattttg cgattcgtac ggtctgctac gcattgaacg gaaaacaata
    20641 tgccttaaaa gaagagttct tgagctccta tgtcaaagaa ttgacagctt ctggtttcac
    20701 gattccgaac agtccaattg ctgtcggaaa ataatttgga caaacgatgc aacgaatctc
    20761 actaaaaaga gcgtgggaca aaagtaaaga acacttttgt cccacgctct aaacacgtat
    20821 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttgaaa
    20881 agcaattttc gtgaattcct tcttatttct tggagttaaa cgcttttgtc ccagcctctt
    20941 tttcaagata aagtcaaaac tggtacgctg ttttttctac ttattttttg attatctttt
    21001 agtttctcgt tctttagctc acgatatcga tttgttcttt taattcttcg attttcggag
    21061 acaatatatt acgacgttct aattcatgca ccatgaagtt tacggcttta agcagctctt
    21121 ttcttgtaat gattcctttg aaaatccgat cttctgtgac aaccggtaaa aaaggcgaat
    21181 cgactaacaa atggagtact tcttctagtt cccaattttc atcaattgtc ggaacatctg
    21241 tttctgtcac atcagcaacc gttagtcctt ctaacgggtc catgctgatt gtttgcaagt
    21301 caatcatttt attgaccaca tctgataaac ttactagtcc tacaaagtga tcatcttttc
    21361 ctaatacagg aattttggaa taaccgacct tcgataacac aagtaaacca tgggacaatg
    21421 gatgatgata cataacattt gccacatttt ctgcaggaat caaaaacgtt tcctgatttt
    21481 ccagcagcat ttctcttact ataggtccaa tcatctgatg gcctccattt ctatttcatt
    21541 caacactact attttaccga aaccggatgc aaaattcata aaataaagct tatttttatt
    21601 aaaaattaga gtttttttat tcagcgctct ctcgaaaaag aaaaggccaa ctcttcaact
    21661 gcctgatggg cacggttata gtattgaaca gaaatttggt catctaaact ttcgataaga
    21721 gcatatgatg ggatttggat cgggcctcta ggctgagaaa tgctaccagg attcaaaaac
    21781 aggattttct gatccatctc acagcctatt tgatgggtat gaccaaaaaa caccatatcg
    21841 gcagttgcag ctcgagcttc gattgctaac tgtgtcaaac caaatcggac attcgataaa
    21901 tgaccgtgag tcatgaatac tgtatcctga ccagtttgga tcactttttt ctgttcgaat
    21961 cctggtccat aatcacaatt accttgaacc acttggaatg tatcccataa tgggtcagaa
    22021 gcttctaatt ccgaatcacc acaatggaaa aagatatcca cttcagaacg ataacgatct
    22081 actaagtcaa cgaggatatc tctgtctcca tgattatcac ttactactaa ataccgcatc
    22141 tatctctctc cttccagcca ttgtttccac tcggcactca attttttcat tgcttgagcc
    22201 cgatgactga tcttgttttt ttcttcgctc gataattctg ctgctgtttg tttcttacct
    22261 tctgggataa ataaaggatc atacccaaag ccattttcgc cagatggtat acgggctact
    22321 cgaccgggcc attccgcttc tactacaagg ctgtcttttt tgggtgccgc gaatactaac
    22381 gtacaatgga attgtgctgt tcttttttca tccggcacat ctgtcaattc atataataat
    22441 ttggcgttat tccccgcatc acttttttgt tcccctgcaa aacgtgcaga atagattcct
    22501 ggcataccgc ctaaagcatc cactttcaaa cctgaatcat ctgctaagac tggctgatca
    22561 agcaattgtg caatcgtttc tgcttttaat cgggcatttt cttcaaatgt gctgccagtt
    22621 tcttccacat ctggcaattc aggatagtca agtaatgttt ttacatgata tcccgcttct
    22681 ttgaacatat ttcgaaactc ttctgctttt cctggatttc ttgtcgcaat gatgattgtt
    22741 tttgtttctt ctatccgctc atcattttga gcaaactctg ttagcaaagc atgtttttgc
    22801 tctttgatca aatcttcaat cgcgtttttt ccaaaaaaga gcatttcatt caactgttca
    22861 ccatcaaacg ttgcttcttc tccagttcct tgaatctcca cgaattttcc agattctgtc
    22921 attacaagat tcatgtctac aagtgctgcg gaatcttctt ggtaatccaa atctgtgacg
    22981 caagtcccat ctggtaagat tcctacgcta actgcagcaa gatgttcttt tattggatct
    23041 tctgacaatt ctttttctct taaaagtttt tcaatggcta atttcaatgc cacaaatgcc
    23101 ccagtgatac ttgctgttct agtaccgcca tccgcttgga tcacatcgca gtctacgatg
    23161 atactacgtt ctcctaattt ttccaagtct actactgcac gcaaggaacg accaataaga
    23221 cgctggattt ccattgttct accagaaagt ttccctttgc tgctttctct acgatttctc
    23281 gtactcgttg ctcgaggcag catactatat tctgctgtca cccatcctgt tcctgtttct
    23341 ctcaaaaaag ggggaacaga attttctatt gttgcagaac aaataactgt tgtatcacca
    23401 aaacggatca ctaccgatcc ttctggatat ttgaaaacat ttgtttgaag ctccagtttg
    23461 cgaagctctt gggcttttct cccgtcatga cgcatcctta ttttcctccc aattcgatat
    23521 gttctacatt taagtgtccg attccaagcc agtcttcagc tatttcctcg aaaagtttgg
    23581 cagagccagt tgtataaaac tggcataatg tccgaccatt ttgcggtgaa ttgctcaaat
    23641 tgaaataatc taacagcata gatacttcac ctactgtttc tgctccagaa tcgatcagtt
    23701 gaacattttc tcccattaca ttttgaatga tggggcgtaa taatggataa tgggtgcatc
    23761 ccaaaatcaa tgtatcgatt tttttagtgg ttaaaggagc taatgtttct gccacaattt
    23821 ttttcgccac cgatgaatgg tattcattac tttcgacaac tgaaacaaat ttgggacaag
    23881 caagacttgt cacagtcaat tctggtactt tctctttcag tgctttttca taagcttgac
    23941 tttttaccgt accaatcgta ccgataatgc caacttgttt attttgtgtt ttttttactg
    24001 ccgctctagt accgggaagg atcacaccga tgactggaat agaaagagca gctttgattt
    24061 cttctaaagc caccgcagtt gcggtattgc aggcgatcac cagcatcttg attccttgct
    24121 ccaccagata atccgtcatt tcccaagtat actggattac ctgttccgcg ggtctagggc
    24181 catatgggca gcgtgctgtg tctcctacaa ataaaatatt ttcattcggt aattgtttca
    24241 gggcttcttt tactacagtc aagccgccga cacctgaatc aataaatccg atagggcgat
    24301 tatctgtcaa tcgtatcatc ctttttttca ataaattcta tcctccctat tgtgtacttt
    24361 ttagcgtgaa ttttcaagtg ctcttacttg cttaaaacaa tcgaaatctc tttttttcga
    24421 atggaaagag gaaaagcggt tgtgacttta gtttgccaca accgctaaaa gaatcatcta
    24481 ttaataactg taccgtctct tttccaagta accggaaagc agagaaagcg cgtaacatac
    24541 cacgaagtac ataagtgtca tgatgatgaa catcggaagc acataattgg tgttctgtcc
    24601 ataaataata cgtgcctgat gagaaagttc aggtaaagaa atgattactg ctaatgaagt
    24661 atctttgatc agtgaaatca attggctcac aattgccggg atcattgatt taaatgcttg
    24721 cggtaacacg atcgttcgca tcgattctac ataagtcagt cccgttgata atcctgcttc
    24781 catctgtcct tttggaacag cattgagtcc agctcggaaa atttccgaca acatagctga
    24841 ctcaaaaatc gttaatgctg cgacagcgga ccagaagatg ttcatctgaa tgccgatttg
    24901 aggtaaggca aagtaagtga agaaaatgat caataataat ggaagattcc gtataatatc
    24961 aatcactaca ccaaggattt tggaaaagaa agggatattc gcaaaacgaa tcacacctgt
    25021 cagaccaccg atcaagaaac taaaaatgat cgaaaacaat gaaacttcaa cagtaacctt
    25081 taatccttcc aataaaaaac ggatattcat ccatgaaaaa gcgccgctaa aatccatgta
    25141 tttcccctcc ttctatgaac gaaccgccca ttttttctca agataaacca ttaagtaaga
    25201 taacggtagc gtgataacta agtaaaataa cccaacgatg atatacgtat cgaaggtatt
    25261 gaatgtttca ctagcgatca agtctccttg atacatcaaa tctaaaccag ctacgattgc
    25321 taaaatagat gagtttttta cgaggttgat aaattggttg cctaatggcg ggataacgat
    25381 tttgaatgct tgcggtaaaa caatataacg catgatttct gaataggtaa aaccagaaga
    25441 tagtcctgct tctgtttgac cttttggtac ggtttgaatc cctgaacgta ctgtttcagc
    25501 aataaaagca gatgtataga tcgttaatcc aatcgtacct gcttggaatc catctagatt
    25561 aaaccaataa atcggtacga caacataaaa aaacatcacg atgattaata atggaatgtt
    25621 tctaaaaaat tctacatatg cctttgctaa ggcgctcact actttattct ttgataattg
    25681 gaagattgcc attaacgttc cgataatcaa actaaacaca agtgctaaga cactcgaaaa
    25741 caatgtgatc ttgaacccgt cgacaaagat tggtccataa gtttgtatca attcgctcat
    25801 tgcctctccc ccttactcta cttttccttc agtttcattt ggaaaccatt tttgatagat
    25861 tttatcgtat gtgccatctg catgcatttc ctctagcgct tggttaacag ctttcaagaa
    25921 gttttcttgc cctttgttga tcgcaatccc gtaaggctca ttagtaaacg ttccaccaac
    25981 taactcatac tctggatttt catctgcaat ccctaacaaa atcgcattat ccgtagtcat
    26041 cgcgtctccc tgaccagatt gtaaagcagt aaatgcttct gcatagtttt ctaattctaa
    26101 aatttttgca tctggtgcat gctgtcggat attcgcagcc gatgtagaac ctttgactgc
    26161 tagaaccgtt gtagaagcat tcaaatcatc tacacttttg atttggcttc ctttctttac
    26221 caacaaagct tggccagcat cgaaatagac atctgaaaag tcaacctgtt tttttcgttc
    26281 atctgtgatc gtcatcgttg caataattgc atcgatattt ccgtttttca ataatggaat
    26341 ccgagtttta gaagtgactt caacaaattc tgtttttcca ttgtcaccaa ggatcttttt
    26401 agtgattgct tttgcaatat ctacatcaaa tccttgtaca gttctgctct caatatccat
    26461 cattccaaat aatcgagtat catacttgac accccagatg atttcattgg tagatttgct
    26521 tcgctctaaa atatcttcat ttgccaaccc atttccttta catccggcta gtgcaagaaa
    26581 agcaatgcct aaaaggaaaa tcaggcgtat tttttttaac ctgcgcatct ttttcctcct
    26641 atcaatgatt gataacttta ctgataaatt gctgtgctcg gacttcattt ggattttcga
    26701 aaaagtctct cacatttctg ctgtcttcca atacttctcc atcagccatg aagataacac
    26761 gatccgccac ttctttggca aagcccattt catgtgtcac gacaatcatt gacataccgt
    26821 catgagccaa ttttttcata acgtctaata catcaccaat catttccgga tccaaagcag
    26881 aagtaggttc atcaaacagc agcatctcag gattcatcgc taaacctctg gcgattgcca
    26941 cccgttgctg ttgtcccccg gataacatgg aaggataaga atctttctta tctaacatat
    27001 tgacagtttt caaaaatttc tccgcgtttt tgactgcagt tgcctggtct tgcttcaaca
    27061 ctttgattgg tgctaatttg atgttttcca gaacagtttt atttgggtac aaattgaaat
    27121 gttggaaaac catgccgatg ttttttcgga cttctgtcag ttttgtcttt ttatcatgca
    27181 agtcagtatc attaatcagc agcttacctg aagagatcgt ctctaatcca ttgatacagc
    27241 gaagcatcgt gctctttcct gaaccagaag gaccaatgac aacgacaact tctcctttct
    27301 cgaattcgag attgatattt tttaatgcgt ggaacttgcc ataatattta tccacgtgat
    27361 caaatttaat cattgccata gaattacact cccattatta tttagtaaga tttcttttaa
    27421 agatgtataa taaaatacat tatatctgat agtttagtaa tttgtaaagt ttcttagaac
    27481 gtttattctc ccataaatat ttcttccatt tctaatctat tagttatttt atcctctttc
    27541 cttgtttttt ccttaggaac tgttgtttct tttcaggaaa aacggtaaga tattaagtgc
    27601 tcagacttac aaatcgtaaa gaggtgtctt ttttgaaaaa tattatttta acgggagatc
    27661 gaccaactgg gaagctgcat ttagggcatt acgtcggttc tctaaaaacc cgagtggcta
    27721 tgcaggcaga tgaagacaac caactgtttg tcatgattgc tgacatgcaa gcactgacag
    27781 ataatgctaa aaatcctgag aaggtttcat caaatgtctt gcaagttgct ttagactatt
    27841 tagccgtggg attagatcct gcaaaatcta ctttatttat ccaatcacaa attccggaat
    27901 tagctgaatt aaccatgtat tatttgaact tagtcagcgt aggacgcgtt cggcgcaatc
    27961 cgactgtcaa aacagaaatc gaacaaaaaa aattcggtga aagcgtgcct acaggattct
    28021 ttatctatcc tgtttctcaa gcagcagata tcactgcatt caaagcgaat cttgtacctg
    28081 ttggtgagga tcaaaaacca atgctagagc agacacagga aatcgtacaa agcttcaatc
    28141 atacatatgg tgaagtcttg gtggaaccaa aaggcgtatt cccaccaaaa ggaatgggta
    28201 gattacctgg aatcgatgga aatggcaaaa tgagtaaatc gttaggaaac ggtatttata
    28261 tctccgatcc tgccgatgtc ttacagaaaa aagtcatgag tatgtatact gatccaaacc
    28321 acatccatgt acaagatcct ggacaagtag aagggaatat ggtcttcacc tacttggatg
    28381 tattcggaac agataaagaa gcaattgaag aaatgaaagc tcattaccgc cgcggcggac
    28441 ttggtgatgt aaagatcaaa cgatacctca ttgatgtatt ggaagctgaa tttgcaccga
    28501 tccgtgcgcg ccgagaagaa ttggaaaaag atccttcagc tgttatggaa atcttacgta
    28561 aaggtagtga agaagctgct aaagtagctg cacaaacact ttctgaagta aaatcagcaa
    28621 tgggaatcaa ttacttccat taattcattt actctaaaaa agcaggctgt aaaggcctgt
    28681 ttttttatct cgtctctgtg ctattccttt tttaaaatca aaaaaagaga tgtgacgaag
    28741 ttgtgtcaca tctttttttc aaatgtatgg tcttcaaaag ctacttgatc gttacttact
    28801 atattttcct tcttagcgaa tacttgcctc taggttttct accaacgctt tttcgacttt
    28861 ttccattgct ttattgattt cttcatctgt caatgttgct tctgggttga cgaatgtcag
    28921 gctatacgcc atggatttat gtcctttttc aatgttttct ccttgataga catcaaataa
    28981 ttggatatct gttaaaaatc ttccggcagc agaacggata acttctgtta attcctgatt
    29041 tgtcacagtt tcttttacaa gcaaagcgat gtcccggctg actgctggga attttgtcac
    29101 tgcttcgaaa acgatccctt tttgagcagc ttccactata gcagataaat caaactcagc
    29161 aacatatgtt tctggaatat cataactttt tgtaatcgtt ggatgcactt gaccgacaaa
    29221 accaataaat ttttcgccaa ggtaaatcgc agcagttctt ccaggatgca tttccttcat
    29281 ttctgtcact gtttgataat ggacatcttc agtgatgcct agttgttcaa ataagttttc
    29341 taaaagaccc ttgatggtga aaaagtctac attttgagct ttcgtctgcc aatctttttc
    29401 ttcccaaata ccagaaatag cgattgctgc atgttttact tcttgcggca gatgtgtacg
    29461 tggatcattt tcttggaaga acacccgacc aatttcatat aaagcaatct ctgtattttt
    29521 tcgggcagtg ttataactga tatcatctaa tagtccgctg atcaaattca ttcgtaatac
    29581 agaacgttct tcgctcattg gccagtccaa acgtgtaata ttactttcat ttataacaaa
    29641 ctgtcttgat ttttcttctg ttgtcaatgc ataactgatt gcttcgctca atccgcttcc
    29701 ttcaaagatt gtacgaattt tacgagtagt ttcttgttct tttgacaagc ttccagcaac
    29761 cgtttctcca cttggaagtg tagatggcaa ctggtcgtat ccataaatac gagcaacttc
    29821 ttcgataata tctgcttcga tttggatatc ccagcgtctt ggtggtacta ctacggaata
    29881 aacatactcc ttttcttcat agctaaatcc aagtgcttca aaaatctcat ttacttcttt
    29941 aacgacaaga gatgttccta aatattgatt gatgcgttca agtgaaatct tcactgatac
    30001 gtcttctgct ttgatctctg agcctttgac cgcgccttgt agaacttccc cacccgcaag
    30061 agaaacgatc attgctgcag caacctcact ggcttctcca acagtcgcat ggttgattcc
    30121 tttttcaaaa cgtgcagagg attcactacg caaattaaat tgttttgctg tacgacgaat
    30181 cgaaattgga tcaaataaag cagattctag tgcaactgtt gttgtttcat tcgtgatctc
    30241 tgagtctaac cctcccatga caccagctaa agcaactggt tctttgccgt tagtaatgac
    30301 gagattgtca gttgtcaatt cccgagattc tccatctagt gttactaatg tttcattttc
    30361 ttttgcacgg cggacaaaga tctcttttga tttcaatttg tcgtagtcaa atgcatgtag
    30421 tggttgtccg aataaaagca aaatatagtt cgtcacatct actacattgt tgattggacg
    30481 aatgccttcg ttcatcaaaa gattttgcag ccattgagga cttggctgga ttcttactcc
    30541 ttcaatgatt ctgatttgat aagcaggaac tgcttcttta tcttcaactt ccacttgaat
    30601 tttatcgcta gcaggacgtg aggattctac cagcttttcg tcatcgaatg taggagtctg
    30661 acgataaatt gccccaactt cataagctac gccacgcatt gacaatgcat ccgcacgatt
    30721 cggtgtgata gataattcaa taatcgaatc atccatatcc aaataaggaa aaactggatc
    30781 gccatttaca gcttcttgtg gcatataata gataccttca gcgtattctt tcggtataac
    30841 actatctgaa tagccaattt cttgtaatga gcaaatcatg ccatttgata cttggccacg
    30901 catttttcct tttttgattt ttacattacc agcgatacgt gagttaggaa gtgcaacaat
    30961 cactttgatt ccaaccttca cattaggcgc tccacaaacg atttgagaca gttcgtcttc
    31021 gccaatatcg acttgacaaa tagaaaggtg atcagaatct ggatgtggta cgcattcctt
    31081 gacttcaccg acaacgattt ttttcaagcc ttcttctgga accgttactc cctctacttc
    31141 gattcctgtc acagacatct tatccgctaa ttcttttggt gttacattcg ataaatctac
    31201 ataacgattg agccatttat atgaaactaa cattttctta ctcccccttg aactgactta
    31261 agaaacgtaa gtcattttga tagaaattac ggatatcatt cactccatac cgcaacatag
    31321 ctacgcggtc tggtccaagg ccgaaagcaa agcctgaata ttcttctgga tcgattcctg
    31381 acatcttcaa cacattcggg tgtaccatgc cagctcctaa aatttcgatc catccagtgt
    31441 atttacatac gttacaaccg gcaccaccac atttgaaaca gctgacatct acttctactg
    31501 atggttccgt aaatgggaaa taacttggac gcaaacgaat ttcacgatct tccccaaaca
    31561 tttttttcat tacgacttct aatgttcctt taaggtcgcc cattgtgatg tttttatcaa
    31621 caactagccc ttcaatttga tggaactgat gactgtgggt cgcatcatct gtatctctgc
    31681 ggaagacttt ccctggcgag atcattcgta gagcaccctt agagaaatca tgtttttcca
    31741 ttgtccgtgc ttggactggt gaagtatgtg tccgaatcaa tatctcgtct gaaatataga
    31801 aagtatcttg catgtccctt gctggatgat cttttggcag attcatccgt tcgaaattgt
    31861 aatgatcaga ttctacttca tatccttcta cgatctgata gcccataccc acaaaaatat
    31921 cttcaatttc ttccatgatt tgtgtcacga tatgacgtgt accatgtttt acttttctac
    31981 caggtagtgt cacgtctatt gtttcttttg ccaaagctgc atttaacgct tcttcttcta
    32041 atacttgttt ttttcgctcg atctcttctg tcaaaagatc tcggatctca tttgcgaaac
    32101 ttccgacttt tggtcgctct tctggactta gatctttcat cccacgtaat acttctgtga
    32161 tcggtccttt ttttcctaac gtttcaacac gtatttgatt taattcattt aaatcttgtg
    32221 ccgctgtgat tttgacgagc gttgcttctt tcaagctctc taattgtgct tgtagagaca
    32281 tgtgcttgtt cctcctttaa attccaaata caaaacaaaa agacctcatt cctgttaagg
    32341 aacgaggtct tcgcgttacc atcctaattt gcggataaat gacttacacc gcactcttac
    32401 ttcgttaacg acaattgccg gtctgatttt atcagacgaa ctcggaaagt gaacttcatt
    32461 tttcaaacac aggatctgct ttcagtctgc gacagatctc cctgaactgg tttagccaaa
    32521 ctactctttt tcgtcatcgt tctttactta ttcgtatgga ctatagttta caagattttt
    32581 tttacaaaat caaccgcatt cttcctctgt gatccatttt tcgctccaaa cttgtatttg
    32641 acgcatcgct ttttgcaagt ctgctccttt ttcagtcaag agatattctg aacggctctt
    32701 ttcgctacag gcaaagtttc gagtgactat tccttctttt tctaattcct tcaagcgttc
    32761 gactaatact cgatcactga ccatcgggat ttttgccgca agctcaacaa agcgttgcgg
    32821 accgttttcg agtagtacat cgataatcaa tccgttccat ttttttccta aaatagagaa
    32881 tgttttttca aatttagggc ataaaacgaa ttctgtctgt gttagctcca tcatactcac
    32941 ctcttcccag agtatagcat aatccttaca aaacataagt caagtttgct caattataat
    33001 tatttgttaa tgtttataat ttgtttcact cgaagcaaaa ttaggaaaac attttctact
    33061 ggcttattca aagcttctga cattgcttgt tggtaaagtc ttagctgtcc ttgatatttt
    33121 tggacgattg catctatttc tgctggatca tccggatgta aaataaaatc tgttttaaaa
    33181 tcatagatat tgagatgatc tttttcttct aggtatccat cgacgattcc gtggattaat
    33241 agttcatctt cttgatttgg atagtcttga aatacttcgt cagcaggaag cagcatggaa
    33301 aaaggttgtt ctcgttttac gttttctttg ttggcaatca gctgttgacc aagttctgtt
    33361 tgaaagaacc atataataga agaaacatct acttttttcg cgactttttc gtctactaac
    33421 cgtttcctta ccaattcctg taatttttga tggatactct ctgtagttgg catctctaat
    33481 ggcagtaatt gtaacaaagc atgcgtagca gttccaacag cagctgctga cacttgccga
    33541 tctttttgca aaaattttgg ttctgctagt tgttcttgcg tataacgaaa ttgacgggat
    33601 gctgctttgt tctgactgct ttccagagtc aaacgtgatt cttgtgtatc atctggatct
    33661 tcgaataatc gtttgatttc agatacagat tgataacttg tggtttgtga agaagcttga
    33721 taaggatatt catatgccag acgtttttgt aacgaatctg ctaataaagt ttcatcttct
    33781 ttcatctgct gatctaagtt aaccagttct ttttcagcaa gcaactgtct ttgttcaatg
    33841 atcgattgct gattcatcca tgaaatagaa aactgtgcat ttgaatgttg aagaagagaa
    33901 gtcgaaattt cttcctctaa gtatttctgc atttcaggat gacggatcaa tccgtaacca
    33961 atccaattca tcaagctgct tctgcccttc aaacgtagcg caggatcaaa aactaactcc
    34021 tcatgatcag ctgcttcaga ccacgtttgg atcatttgct ctttattttt ataggaaccg
    34081 acaatgaaaa gcttttgttc tgcccgagtc aatcctacat acaatttacg catttcttcc
    34141 gacaacaatt tgttttgctt tgccaatttg atggcttgaa acggtaacgt atcatataac
    34201 actcgtgttt caggatccat atagcgaatt cctgctccaa gtttttcttc aaaagcatac
    34261 cgattgcgca agtcttgtaa gttgaactcc tttgtcatat ctaacaaaaa tacaactggg
    34321 aattccaagc ctttacttgc atggatcgtc ataacgcgaa cagcattatc ttctattgaa
    34381 atgactggtt ctgctaagtc tttatctttt tcttgcattt tttcaatgaa acgcacgaac
    34441 tggtataatc ctctgaaaga actttgctcg taagctttgg cacgatcaac caatgcataa
    34501 agattggctt gtctctgtgc ccctgctggc agaccgacca catactctag atatcccgtc
    34561 tcataataga tatcccataa caagtcagtg atcgaagaac ggcgagcaag ttctcgccaa
    34621 tgctctagtt gcttgccaaa atgttccagc ttagctgcta attcatcttc gtttgatgct
    34681 tgataagcaa gcacagcatc atagtaagta tgtgcacggt tagctaatcg aatgctggct
    34741 aattctggtt caatcagacc cacgatcggt gagcgcaata cagccgcaag cggaatatct
    34801 tgatagggat tatcgattaa ttgtagcagc gagatcatcg tgcgaatttc tgtcgcttga
    34861 aaatagtttt gagcatcatt catctctaat ggaatatcca atgttttgaa aatctctaaa
    34921 atcgttaagt tgttcttctt tgtaggtgtc aagagaacaa tgtctttgta ctctatcgga
    34981 cgactttttt ttgatttttt atcataaatc ataaaggagg aatcaattaa ttgacgaatt
    35041 tttagtcctg tcataaaaag ttcgccttct gttttgtctt ccagtatatc atccgtcgga
    35101 atctcaatct ctgattcttc ctgttctttt tcatagatca tgatttccgg ctcaaactgt
    35161 tcattctctg gaaagtctga aaatccttgg atcagtttgg ctgcttcgtc ataattgatt
    35221 tgacccactg ctggatccat tagttgttca aaaataagat tcgtaaaaga aaggacttct
    35281 tttctagagc ggaagttctc agccaaaacg attcgacgtc cgccttcttt ctttgagaaa
    35341 ttctcgtatt tcccgataaa taaactagga tctgctaaac ggaaagaata gatggattgt
    35401 ttgacatccc caaccatgaa catatttcct tttgtatcat cgggttctct tagccagtat
    35461 aaaatggctt cttgtaattg gttcacgtct tgatattcat ctaccattac ttctttgaat
    35521 ttttttcgat aatgttttga agcttcactt ggcagccata catctttcgt tttttctgtg
    35581 agaatctgca acgctaaatg ttccagatca ttgaaatcca gcacaccttt ttcccgttta
    35641 cgcatactga aaccgtccat gaagctttgc gtgacttttg tcatttcttc gactaacggt
    35701 agtgctttgt ccgttaattc ttccatttgg cttggggaaa caggaaaata acttttactt
    35761 atctgctgaa tcagtttttt tgctttatcc ctcatgccct tgacttcatt ggaaatttct
    35821 ttcaattctg ctttccgact gctcttaaaa gttgaaaaag ttagttgttc caaaccagca
    35881 taagcttctt ctaaccgatc ttgcgagaac gcttcataga tattcttgat ctgctgttgt
    35941 tctccagcta cttgctcatg cattttttct aatccttcac cttgtgctaa ctgagtcatc
    36001 tgttcatata actgtacaca ttggtatata tctgccaaaa ctaaaggacg gatttgtttt
    36061 tgatataatc gagatttcgc tagcccctca ggcaaacgat aattatcgct taactgttct
    36121 agccattttt gggggtcagg gttggcacgg gcaaactcat aaagagaaaa caccagattc
    36181 gtcaagccgt catctgaacg atcattagaa aaattcattg ttaattggaa aaatcgttca
    36241 tcattttctg cataaagtgc ttctctcagc tcgtcccaca catcctcctt catcaagatc
    36301 gtttctgttt catctgttaa catgcgaaag actggatcaa tatcaattaa gtaataataa
    36361 cgtcgaataa ctgtcaggca aaaagcatgt aaggttgaga tattcgctgt gggcaataaa
    36421 actaactgct tagtaaaatg ctgtctacgt acagggtcac tttcgctatt gacggattct
    36481 tgtaatgcct cttgaattct ttctttcatt tctcttgcgg cagcttcagt aaatgtcaca
    36541 atcaataatt catcgatatc aaaacccatt tttagttttt caatcactct tcgaacaagc
    36601 actgtagttt tacctgatcc ggcagacgca gagactaata gattatctcc ttgatcaaaa
    36661 atcgcttgcc actgatcatc tgtgaatcgt tcattttgcg gttttaaagg aatttgattc
    36721 actcttcttc actccttttc aatattctac gcagtgcttc ttccttatct attttctcca
    36781 gacggtggta attattttct ttcagcatca catcaaacgt acagatgctt ctgaatggac
    36841 aaaattcgca ggcaatccgc tgtttatctt tataagctgg atttagtttg atttctccgg
    36901 aagtaatcgc atctccagct aaccgcatat tttcccgatt atgcgccatg aatagttgca
    36961 gttcttcttc cgtgaaaaat ttgtctttac tgaaactacc tttttggtat tcatctttcg
    37021 catttttttt cactggatag atcagtgatt gttcttttgc ttccagtgaa tgatctagta
    37081 cttctaaaag agcgggatca tcgacaaaaa cgccatcata actaaatttt ttcaatgtat
    37141 tcttctctac atcatctgtc tccgtcaata caggattatg aacatgtaaa taatatgatc
    37201 ctgctggttt aacagccgcc tttccagtca aacgtaccgc atccatcaat gctacgtcca
    37261 gatacgtcaa taattgcata gccatgccat aataagcttc tgtgacatcg aatgaacgtc
    37321 cgctagattt gtaatcaacg acgcttagcc aagtatcttc ttgttcagaa gctacatcga
    37381 ttcgatcgat tttccctcga acatggagtt ttccaccatt actcaacggt aattccagac
    37441 ctgtgatacc ttttgctcca gcaatctgac caaatagaat ctctgtctga agaggatgca
    37501 tgcctgtatt tttgctctgt ttttgcaaag cccatgtcac tttttgaatc gtctgtccta
    37561 actggtagcg gatatagttc attcttgcag aactatccaa tatctcaaac cgaagttctc
    37621 cgaatacttc ctgcagcact ttttcagtaa agtctctacg ctgcgcatcg gacatattca
    37681 ctaaggataa ttggtttgaa aacagtagct tgaagaaacg atccaatgat tcatgataaa
    37741 aatcaccagt tgctgctgga gttagaccat aaacagttcg ttctttcaat ttcaaaccaa
    37801 attgcatgaa gtatttgtat tcacaattat aaaaattctc catacgtgaa attgaagtat
    37861 aaatctcttt tccataaagc tcttccgcca ttttttctgg catcgaaaca ggaacattta
    37921 aatgttcctg actttcaaaa atctgtaaag caaaatttcg ccatgacgaa ttcagtacta
    37981 attctttgag cttttgccag tgctttggta caggtttttt ttcatctaaa gctaatcgat
    38041 aaatcctatt gactgtattg attccgcttc tgtaagtccc tacataacaa ttagggtcgc
    38101 tttcgatcgt caaatgctct tttctctcga cgggaacaga taaatattct gtgattctac
    38161 gcaaataagg agacatcttc agcgaaccat ctgtgtcata gctttgtgca taactaagat
    38221 ataatcgatc tgttccggaa agaaaaacaa gataggcttg gaaaggctca aaagagatac
    38281 tttcctttga tgggtcgaat aaaaactgtc catctggcaa ggcctgattt aatacttctc
    38341 gttcttcact ggacaacaat ccttttgtct cttttcggtc tggaaacact tgatcattca
    38401 accctaatgc aaaagtgacg gcagcttgat ttggtcgaac taattccaaa cgattgattc
    38461 gcacttggtc gattgcggta gggattttac cataacttag attttctaga ccacttgtaa
    38521 tgatttcttg gaacagtgac cagtcaaaag ccgattcacc ataaattact acatattcat
    38581 ccaacagatc aatcagcgct ccccatgtct gctcatggtt tcttgccgct tctaagtttc
    38641 ctctgtctac ttcctgatta cgccaaaaca ataattgttg ctgtacgcca ctttggatca
    38701 aaaattgata aaaaacattt gcggcctctt gtcctgtttt gcttttcttt atttttttca
    38761 aaaatactgg tacagctttc tgaaataatc gtctaatcgt atttgactgt tcttccaaat
    38821 actgtacgtc ttcaatctgt tctgcttcaa aatcatacgc cacaaactgc cagtcttttt
    38881 cacgagtcca ataagttcct tgataattgt aagcaagtgc tacattttca gtgatgtcca
    38941 gttttttcct gaattcatct cgctcttgtt tccattcatc aagttcggtt tctgcttccg
    39001 tcgacagaaa taattcagtt ttgaaaaaac gcataatatc acttagccga taagaatagc
    39061 ggtcaagagc gaacagactg ttgatccatt cgatcagcgg atgattttcc atcatttgtt
    39121 cttcatcgat ataaaaagga attgccattt cttgaaagat cggccgaagg atatttccat
    39181 ataaagacat attttttgtc agcacttgga tatcacgata acggtagttt tcttcactga
    39241 ctagtcgtct gatttcaaca gctaattgtc ggacttcttc agttggattt tcagcttccc
    39301 acaaatggag atgctcttgt ccccctattt tgtgtactgc cgctttcttt tggctagatg
    39361 tctcttccca cattgtttgc agcagttgaa gctctgtcgg acgagtctgc tctggtgcaa
    39421 atcggtccac taatataggg actttccgtt ctcttgcact ttgaaaaaga cggaaataaa
    39481 gctttccagt ctcatgaaag agcgtaagta cttctggaag atccgctgta taaggacgat
    39541 ccagcaataa atcgacaacc aattgcccct tttccatcat tgcctgcagt aattgatact
    39601 cttgaccatt gattcgagaa aaaccactga caataaattt gacgttgttg aaatcttgtg
    39661 tctgcatata atttgctagt atagatagtg catcttcatt tgttagtgca cgttgagtca
    39721 attcgctgtc ataagctgaa aaaatcagct tcaagtcttt cattttaagt tgctggtctt
    39781 ctgcattagg tgaatcacca aataaaagca tcaggtcttc tggttgaata tttcctgtac
    39841 gcaattcact gtagagttct tgtaattgac gaataaaccc tcttttattg atttctccgc
    39901 gaaagattgt taattcttcc tcattttttt ctaaaatctg tcgcaagatc attgcagatc
    39961 ctgattctga taattcattt cctgtaagaa gagttgtctg ttgcagcaaa taccaagcca
    40021 gacgataaaa actaaatact tgaacgtttg ttgtgctgaa agtttttttt ccgcttcttc
    40081 ttcgcatctc cgctaaaatt tcttgttcct gttcgaattt gctgtagttg ggaacaagga
    40141 aaaatacttg gttacttgca tcctggtcga gccaagcgga tgcttcgtcg atcaagactt
    40201 gacggtgatc ttttttcccg tttccaagaa taaattgtaa agacatgctt ttcctccttc
    40261 aacacatgat acggtgctcc tttattatat caaaaaatac tgaacatttg ttctatattc
    40321 ttcaaattat atccttttaa ggttctgata tttctacttt tttaagtata cg
//
LOCUS       DANJMI010000026        37356 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000026 DANJMI010000000
VERSION     DANJMI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.026"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            19..540
                     /locus_tag="PWU81_001562"
     CDS             19..540
                     /locus_tag="PWU81_001562"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1110269.1"
                     /translation="MHIGDKIHKLRRLNNETMNDLVKQTGLSKSWVSDIESGKKQRAD
                     FNKICAIAKHYKVSLEYFQDDYLDSDEHEDIIPLTDETRVKSVKSLEQSLKYLNNKQE
                     PNLLEIKLKDTDSVPEVYYKGQKLEGLIDVSYHYHTTTDVVAGSTNDINMEYLGEVTK
                     YPDIKTIGHKRDV"
     gene            541..858
                     /locus_tag="PWU81_001563"
     CDS             541..858
                     /locus_tag="PWU81_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL1110270.1"
                     /translation="MKEARPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(1015..1179)
                     /locus_tag="PWU81_001564"
     CDS             complement(1015..1179)
                     /locus_tag="PWU81_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HDL1110271.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGREVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            1282..1803
                     /locus_tag="PWU81_001565"
     CDS             1282..1803
                     /locus_tag="PWU81_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDL1110272.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            1790..2263
                     /locus_tag="PWU81_001566"
     CDS             1790..2263
                     /locus_tag="PWU81_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDL1110273.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKFCRLKIGQNR"
     gene            complement(2270..2428)
                     /locus_tag="PWU81_001567"
     CDS             complement(2270..2428)
                     /locus_tag="PWU81_001567"
                     /inference="COORDINATES: protein motif:HMM:NF024064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HDL1110274.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            2521..3282
                     /locus_tag="PWU81_001568"
     CDS             2521..3282
                     /locus_tag="PWU81_001568"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL1110275.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            3502..4821
                     /locus_tag="PWU81_001569"
     CDS             3502..4821
                     /locus_tag="PWU81_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL1110276.1"
                     /translation="MVAYDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFG
                     KPKRSSINYAPAFSKMEPRASDSRKLDGLNTHFGIFDEIHEFTNYNLINVIKKSRGTR
                     KQPLIVYITTAGYVLDGPLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPR
                     LWIKANPNICLMNFVGMLDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKR
                     NNKTIDIETLKGKKCVGGFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDN
                     NQERIKAWEKVGDLTIIPGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELE
                     NAGFETNEIRQGFLSLGGPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWM
                     PSKQSKSRKIDGFAASLNSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            4837..6057
                     /locus_tag="PWU81_001570"
     CDS             4837..6057
                     /locus_tag="PWU81_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL1110277.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDAEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALIKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGGNEGLKVKFASNVDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGSNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTNSSKSVAENG"
     gene            6092..6769
                     /locus_tag="PWU81_001571"
     CDS             6092..6769
                     /locus_tag="PWU81_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL1110278.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGIDCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAETPQEETLLQKKLKA
                     LNGGKNE"
     gene            6766..7962
                     /locus_tag="PWU81_001572"
     CDS             6766..7962
                     /locus_tag="PWU81_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL1110279.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRQFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     INPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSNWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIINVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTSIANE
                     VPVTNG"
     gene            7977..8300
                     /locus_tag="PWU81_001573"
     CDS             7977..8300
                     /locus_tag="PWU81_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL1110280.1"
                     /translation="MILDPKMDLDEIKNALKIDTDDDDVEVRRAVQAAIAYIRGAIGN
                     DKPSFYKQENDTVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKARYL
                     LFVEE"
     gene            8304..8639
                     /locus_tag="PWU81_001574"
     CDS             8304..8639
                     /locus_tag="PWU81_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL1110281.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVRTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            8636..9031
                     /locus_tag="PWU81_001575"
     CDS             8636..9031
                     /locus_tag="PWU81_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110282.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEKLQQNT
                     PYWDGTKSNGKRGSYMQEHAKDHVVTSSVKNGLIEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9028..9399
                     /locus_tag="PWU81_001576"
     CDS             9028..9399
                     /locus_tag="PWU81_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110283.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EIFGNYEDFASNNPLTIQFNVQVDVWVSTLKEVDAFYFALDEIMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            9415..9975
                     /locus_tag="PWU81_001577"
     CDS             9415..9975
                     /locus_tag="PWU81_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1110284.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGVNVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLAKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEEMTLQKFKDLVNNKAGE"
     gene            10102..10413
                     /locus_tag="PWU81_001578"
     CDS             10102..10413
                     /locus_tag="PWU81_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110285.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     R"
     gene            10437..10628
                     /locus_tag="PWU81_001579"
     CDS             10437..10628
                     /locus_tag="PWU81_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110286.1"
                     /translation="MDQIYKDLIESGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            10628..14473
                     /locus_tag="PWU81_001580"
     CDS             10628..14473
                     /locus_tag="PWU81_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDL1110287.1"
                     /translation="MAGKGQPAGNIKLGISLDSTNFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKKIEDFGDKSTRSARKLDGLKDKLGSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIDKVGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NKVSDAIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGADKLEEWKVPQYAKGTPNGGHPGGPMMVNDGRGAE
                     AVITPNGQAFIPRGRNVVLNAPKGTHVLTAEETAYMTGNKAPRYRYAKGTGFFGNLWN
                     NVKGFAGDVGNKLKDVVGDVWDFVTDPGALARKVLNGLGVLEGLVKYPLDVGKGILSK
                     ATEALTNKITELFSSGSLDTSMGMQGVYKYLADVAVAVMKKFPGFVATSGYRPGDPYS
                     HGKRNAIDIALPGVTGGSPRYTEAANYAFEKFANKIGYVITNGKVRDRSGQSGTGIHN
                     DWRPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTAN
                     LNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFDP
                     LSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAKR
                     SRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKGN
                     SRTDRDLALDIQKILVRRM"
     gene            14474..15346
                     /locus_tag="PWU81_001581"
     CDS             14474..15346
                     /locus_tag="PWU81_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HDL1110288.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSENVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            15343..16392
                     /locus_tag="PWU81_001582"
     CDS             15343..16392
                     /locus_tag="PWU81_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110289.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGADEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            16396..17220
                     /locus_tag="PWU81_001583"
     CDS             16396..17220
                     /locus_tag="PWU81_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110290.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVTSTGSTVSISKISDSYELTLS
                     KGEILWSGTALMHDTQTVQPSKKMWQTVSGFLLVWLPYENGQAIGDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            17238..19370
                     /locus_tag="PWU81_001584"
     CDS             17238..19370
                     /locus_tag="PWU81_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
                     /protein_id="HDL1110291.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKTNDRIDQTNQQIGDLGKLKKMYSNSIDFGDYD
                     YSGNPNLSAKLNASSFSLGTGATVADDNDEIVFTLDGTNQLSKYASKAQVPVAEGKQY
                     TISCEIMLEDGFTGDPSGIRLQHAYLPGGVVVLQTETVPKNELNTWQTLIGTRTVKYT
                     SDVPNEWYPLFRDIRTLKPTGKVRLRKIKIEEGSTATPYQPNLLDDPYWLGKAPLGEN
                     IANKSVTFPIKSSAYSLYQANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRY
                     GNLEPVEGLVDTWGLTFTPQKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGNTRTP
                     NISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEI
                     NSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSA
                     KLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQL
                     SIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            19387..19536
                     /locus_tag="PWU81_001585"
     CDS             19387..19536
                     /locus_tag="PWU81_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110292.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPILNWKPKFNY
                     WEGNK"
     gene            19533..19979
                     /locus_tag="PWU81_001586"
     CDS             19533..19979
                     /locus_tag="PWU81_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110293.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            19981..20118
                     /locus_tag="PWU81_001587"
     CDS             19981..20118
                     /locus_tag="PWU81_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL1110294.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIISKEESA
                     S"
     gene            20155..20448
                     /locus_tag="PWU81_001588"
     CDS             20155..20448
                     /locus_tag="PWU81_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110295.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERSR"
     gene            20471..20668
                     /locus_tag="PWU81_001589"
     CDS             20471..20668
                     /locus_tag="PWU81_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL1110296.1"
                     /translation="MILPDKYYQVIKWTVLTVLPALSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            20679..21698
                     /locus_tag="PWU81_001590"
     CDS             20679..21698
                     /locus_tag="PWU81_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL1110297.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGVNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            21955..22602
                     /locus_tag="PWU81_001591"
     CDS             21955..22602
                     /locus_tag="PWU81_001591"
                     /inference="COORDINATES: protein motif:HMM:NF014505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL1110298.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            complement(22927..23073)
                     /locus_tag="PWU81_001592"
                     /pseudo
     CDS             complement(22927..23073)
                     /locus_tag="PWU81_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            23312..24004
                     /locus_tag="PWU81_001593"
     CDS             23312..24004
                     /locus_tag="PWU81_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL1110299.1"
                     /translation="MNKKIVVSGLIIGGLVLAGIAGKNFADDILLWGGEDNIEQINHN
                     LESLDKALENKEQNISSLNSRLSSNNQELEQAKANVEEYKQKVLTLENDKNQLTIEKT
                     NLENQLSGKNAELQGKQNEINAKIDEINQKIAEINQLNTDWNNRLQTEQNKFNDAQNT
                     VNSLNQQINQLSNEKNTIKNQLQSTEQKLEKAQSENSNLNNYIEKLEKAKSDVEDTAN
                     RSQEIVTEHTGK"
     gene            complement(24286..24358)
                     /locus_tag="PWU81_001594"
     tRNA            complement(24286..24358)
                     /locus_tag="PWU81_001594"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24323..24325),aa:Lys,seq:ctt)
     gene            24739..25308
                     /locus_tag="PWU81_001595"
     CDS             24739..25308
                     /locus_tag="PWU81_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDL1110300.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            25295..26155
                     /locus_tag="PWU81_001596"
     CDS             25295..26155
                     /locus_tag="PWU81_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL1110301.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            26345..27049
                     /locus_tag="PWU81_001597"
     CDS             26345..27049
                     /locus_tag="PWU81_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1110302.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            27054..28889
                     /gene="walK"
                     /locus_tag="PWU81_001598"
     CDS             27054..28889
                     /gene="walK"
                     /locus_tag="PWU81_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDL1110303.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            28886..30202
                     /locus_tag="PWU81_001599"
     CDS             28886..30202
                     /locus_tag="PWU81_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110304.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            30203..31072
                     /locus_tag="PWU81_001600"
     CDS             30203..31072
                     /locus_tag="PWU81_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDL1110305.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            31127..31936
                     /locus_tag="PWU81_001601"
     CDS             31127..31936
                     /locus_tag="PWU81_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL1110306.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(32039..32668)
                     /locus_tag="PWU81_001602"
     CDS             complement(32039..32668)
                     /locus_tag="PWU81_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL1110307.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            32838..34130
                     /locus_tag="PWU81_001603"
     CDS             32838..34130
                     /locus_tag="PWU81_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1110308.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(34206..34868)
                     /locus_tag="PWU81_001604"
     CDS             complement(34206..34868)
                     /locus_tag="PWU81_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL1110309.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            35093..35377
                     /gene="groES"
                     /locus_tag="PWU81_001605"
     CDS             35093..35377
                     /gene="groES"
                     /locus_tag="PWU81_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL1110310.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            35428..37053
                     /gene="groL"
                     /locus_tag="PWU81_001606"
     CDS             35428..37053
                     /gene="groL"
                     /locus_tag="PWU81_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL1110311.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT        12894 a         6308 c         8098 g        10056 t
ORIGIN      
        1 gattaaggag gaagagtaat gcatatagga gataagatac ataaattgag acggcttaat
       61 aacgaaacaa tgaacgactt agttaaacaa accggactgt caaaatcatg ggtatctgat
      121 attgagtctg gaaaaaagca acgcgctgac tttaataaga tatgtgcaat agccaaacat
      181 tataaagtta gcttagaata ctttcaggat gattatttgg atagtgatga acacgaggat
      241 attattcctt tgacagacga aacaagagtt aaatcagtga aatcgcttga acaaagccta
      301 aagtatctga ataacaaaca agaacctaac cttctagaga tcaaactaaa agatactgac
      361 tcagtaccag aggtttatta caaaggtcag aagttagagg gattgataga tgtaagctat
      421 cactaccata caacaactga tgtggtagct ggttcaacaa acgacatcaa tatggagtac
      481 ctaggtgaag taactaagta tccagatata aaaacaatcg gacacaagag agatgtgtaa
      541 atgaaagaag ctagacctag agacgagata gacaaactat acaagaccaa acgatggcga
      601 gacctaaggc aagtagtaat agctagggac ttcggcatgt gccaagagtg caagcgtcga
      661 gggcggaaca caaggggcac gatcatccat cacatagtcg aggcgaggga agacctgtca
      721 ctgttctggt ccgtagataa ccttgaatgt atctgtgtag cttgtcacaa cagagagcat
      781 ccagagagat caggcgggaa gaagaaacca aaacctaaat cacatatcgt taaaatgtat
      841 tcaactcctg aaagataagt ttgcagcgaa atgaaggtag cccccctact ctaaaagatt
      901 aaagagtaag gcttgagaag aacggtgctg tccttccttc gtaaaaagac cgcttttcaa
      961 gttttttgga gaaaaaggaa aaagccgacc aatttaagcc ggctttggac gaagctattt
     1021 cttagtccat ttgtttcctt tttgagaagt aggaggtaat cggtcgcctg gatcaatttt
     1081 tacttctcgt ccgccttgga catttccacc acgaggtccc acttctttat aggttccttt
     1141 tggtttattg tcttcgccgg gtttatagag ttctcccata ggaatccctc cctaaaaaaa
     1201 tttcggcaca gcactgccga taacttaatt ataaggattg tgataacgat ttcaatatat
     1261 cttttgaaag aaggtgatat tatgccgcaa ccagcgaaga gtgcaaaatt acaattatta
     1321 aacggaaacc caaataagaa gaataccgaa gaactccgca agcgagcggc cgcagaagac
     1381 aaattaaaaa tggctactga caaaatcaaa ccgccgttat ggctagattc gctaggaaag
     1441 gatacctttg agtttatcgc tgatgaattg ctgtctgtgg atttaatcag taatccggac
     1501 gtccatacaa tggctctcta ctccaattgg tattcgcaat acgtttcttt agaaaaacag
     1561 cttcgaaaac tacaacgaga gtacaagttg aactatgcgc ttgcgaaaaa ggaggcagag
     1621 gcgagaggtg agccgtttaa tgaacctaat gaattaattg gtaatccgct ctctcggcag
     1681 atggatacag cgtcgcggaa tctccgttct tttggcgctg atttaggact atcaccagca
     1741 gccagagcta agttagctat taagatggct gatgatggtg gtgatgacga tgacgacttc
     1801 taatattttg gatatgtcct acacagaacg tgtggactat tggcaaagct atcttgagga
     1861 gcaagcttct tggggtggct ttttaaaatg tccatatccg gaattgttaa ctacttggta
     1921 tgcggaacga ttaatcgatg gaagcatacc agccagcaaa gaaaatattc aagctgctaa
     1981 acggcatatg cgtgatttgc agcgccaagg aacagatgat tttccttgga tctttgacga
     2041 agaaaaaggt caccggccta ttagatatat cgaaaaaaaa tgtaaaccaa ctgaaggcga
     2101 ctttggttcg tttgttttgc aaccttggca gcatttcata attggatcca tgtacggatg
     2161 ggtacatcgt gatacaggag agcgtcgctt ccgcgaggct cttatttttg ttggacgtaa
     2221 aaacggtaag ttttgccgtt taaaaatcgg gcaaaatcgg tgaaaacctt tatttttggc
     2281 tttcttttag taataattcg atagctttat caagcaattt agacatagga accatagttt
     2341 cttccgacat tttttttaat ccttcgtata aatctttatc aattgcgtta gaaattctaa
     2401 ttctgttttt tagtccgtat ttgtccatgt gtgacactcc tttttatttt actataacat
     2461 ccgattccac cgcttgcaac taccatcaat tgatggtagt atataaaaga ggtgagtaga
     2521 ttgaaaaaat acattattta caaacactta aataaaataa atggcaaaat ttatatcggt
     2581 gtcacaaatg atattggcag aaggtggagg agcggtggga ttgaatataa accaccaaag
     2641 aacgaaaccc aacaccacag aagtttttgg aatgcaatac aaaaatacgg gtgggaaaat
     2701 tttgatcatt tgattattga agaagactta acgatgaaag aagcttttga gaaagaaaag
     2761 ttttatattg aactttatga ctcaactaat aagaaaaaag gttataacat cgcaaaaggc
     2821 ggaaatggcg gaataatata taaagttcac cccaaaggaa tgctagggaa aaaacaatct
     2881 aaagaatttt cttcaaatca cagtaaatgg gcaaagaacc ataagaataa ttgtatgaca
     2941 aacggtgatg ttgtatgggg tgttactcac gaacacccaa aaggcatgtt gggaaagcac
     3001 cacagcaaag aatcaattat gaaaaagaaa gcatatagtg gtgaaaatgc tgtaacctca
     3061 aaaccaattg tcgcaattga acgggatgga actaaaagag aatttcacag cgctaagctt
     3121 tgcatggctt actattcgat tagtacatgt gttttttacc gacttttaaa agatggcgcc
     3181 ccttatgtga taaatcctaa agcgaactac agaaataaag aaaaaatatt agctatcgaa
     3241 ggaattatgt tcaaacataa agaagatacc gaggtaagtt agtagattgc gaaaggttac
     3301 taactaccgt aacgcgtagg agttgaataa atataatgct cccaagagtg tccgacagca
     3361 gataatttgc tgaaaatgta cgctaaactg ggccagaact gactgaccga tgaaaatgag
     3421 ggtgacctcc agagtgcgag ataaaaagct cgcagataat aacaatcgaa aacatcacta
     3481 atttcaggcc tttccacata catggtcgct tatgatgatg aacaaggcgc caacgtttac
     3541 gtattggcaa atgctcgtga tcaagcaagc ttgttgtttg ataaggccgc agaaatggtc
     3601 aaacaatcgc cggcgctctt taagaaattt ggtaagccta aacgatcaag tattaattat
     3661 gctcccgcct tttctaaaat ggaaccacgc gcctcagata gccggaaatt ggatgggcta
     3721 aacactcact ttggtatttt tgacgagatc cacgagttta cgaattacaa tctgatcaac
     3781 gttatcaaga aatcaagagg aaccagaaaa cagcctctga tagtttatat cacaactgct
     3841 ggatatgtat tagatggtcc gttgatgtct tattttgagc aaggtgtgga ctgtttggaa
     3901 catttggaag atgacatcga tgaacggact ttctattatc tggcaaaact tgacagtgcg
     3961 gaagaggctg atgacccaag attatggatc aaagccaatc cgaatatttg tctaatgaat
     4021 tttgttggca tgctagatga ctatgttaag gataaaaaag atccaaaaga atatgctgac
     4081 tggattacca agcaatttaa cttgttttcc gatatcgatg agctgtcatt tgtcgatatg
     4141 cctaccatta aacgaaacaa taaaaccatc gatattgaaa cgctcaaagg taagaagtgt
     4201 gtcggtggtt ttgacttgtc cgaaacggaa gactttaccg cagccgtttt agaatttccg
     4261 cttgaaacag gcgaggtatt cattttgcaa cacacatgga tcccacaagc tagatttgat
     4321 cgagataaca atcaagagcg tatcaaagcg tgggagaagg tgggagatct aacgattatt
     4381 cctggtgatt acgtcaatta tgaatacgtc ttaaattggt ttgtagaaaa ttcgaaaatc
     4441 tatgacattg taaaaatcaa ttatgacaag gccaaggcgc tacgattaaa taaagaacta
     4501 gaaaatgcag gatttgaaac caacgagatt cggcaagggt ttctatcatt aggtgggcca
     4561 atgcaaaact tcaaggaaat gctattggac ggtaaggtga ttttcaacaa ttccaagctt
     4621 taccgatggt atctatccaa cgtcaagctg gtgatggatc gcaactcaaa ctggatgccg
     4681 tctaagcagt ccaagagtag aaaaatagat ggttttgcag caagtttgaa cagccacgcc
     4741 gaagtgttga atatgttggt taatcctgtc ggaaccggga aagtaaccta ttactcgatt
     4801 tccgatttaa tgaatatgta agaaaggtgt ggaggaatga gtattttaga tcgtttgcgt
     4861 tcttttggcc gagcgaagcc gaaagcgagc aaacaagagt attttttgaa tgacccggga
     4921 ttgataccgt atttagtcgg aaaagatgaa atatcagaag ggattttttc cgtaattagc
     4981 cgtgtatcga acgtttttgc gtctctccct ctcaaaatga tagatgcgga gtttggccaa
     5041 ccggacgact gtcctgcata caacttgttg agcgaaggcc ctcgatattt tacaaagttt
     5101 gattttttcc gggacgtgga agttttgaga aactaccaag ggaatgcgta tgtgcagatt
     5161 ttccgaaata tcaatggaga agtagcagat atggcgttaa taaaacctgg tgcttgccat
     5221 ccagtgattg atatggatag cggggagctt tactaccaag taactgcgac tgacaaaggc
     5281 agttataagc aagttattta tgtacattac atggaaatgc tccactttaa acaaccgagg
     5341 tttggtggct tggaaggtac agaccccaca aaagtattaa cgaataccct cggatatgat
     5401 cgagaagtcc gaaaaatctc tttaagtcag cttaaaggag gtaatgaagg gctaaaagtt
     5461 aagtttgcta gcaatgtgga tgaagaagct aaaaaagcta cagttaaaaa cattgctgat
     5521 ttttatcgac aaaacggtgg actacttgtg gaagaaaacg gtgtagaaat cgaacgttta
     5581 caacgagagc tggtagacag caagctttta gatactgata aagtatctcg ctccagaatc
     5641 gcgatggtct acaacgtgcc ggaacatttc attgggagta accagtcgag ttactcttca
     5701 caggaacagc tcaatatgga gtttttgaca tacaatctag taccgaccgt taatcaatat
     5761 gaagcggaac taaataagaa aacactatcg agaaccgaaa aagcaaaagg ttatcgatat
     5821 aagtttaatg tcgcaagttt gctaagagct gacactcagg caagggggca attctatcaa
     5881 atcatgcgac gaggcggagc atactcagcg aatgatgtcc gaagatttga ggacttgcaa
     5941 ccgataaata aaaccggtat ggatgattat catatttccg gagacctata tccaatcgat
     6001 atggatccaa cattaagaaa aacaaactcg tctaaaagcg tagccgaaaa cggttaggct
     6061 tttttagttt gcaccgaagg gaggtgaaag gatgaaaaaa gtgacgttaa gcggcgatgt
     6121 cgtggataac gataccgcgt ggttatatga ctggtttggg attgattgta tctcaccagg
     6181 gaaaatttct gccgctctta cagaagcagc gggggatgaa gtagaacttg atatctcatc
     6241 taacggtggg gatgtcctag cggcaagcga aatatatacc gctatccgtg cctatccggg
     6301 aaaggtatcc ggaaatgttg tgagtattgc agcaagcgct gcaagtgtaa tcgcttgtgc
     6361 ttgcgaaccg cttagaatct cgccaacagc acacatcatg attcacaacg catgggtgac
     6421 cactagcggc aacgccgagg aattaaaagc caatgcggaa atgttaagca gtgtggatga
     6481 gtctatcgtt aatgcttacg agatcaagac aggactcgac cggaaaaagc ttgctgattt
     6541 aatggcaaaa gatacttggt taaacgcaca aacagcagtg gcggaaggtt ttgcggatga
     6601 aattatgttt gcagaagcac cagtaacggt actcaatgcc tctcaaccgg ttattccaaa
     6661 aaacgcggta actaagttga aaaatctaat actcaaagcg gaaacaccgc aagaagaaac
     6721 gctcttgcag aaaaaactaa aagccttaaa tggagggaaa aacgaatgaa tttagaacaa
     6781 ttaaaaaatg cgtgggtcga ggcgggaagt aaagtctctg atttaaacgc gcaactcaat
     6841 gcagcattgg ttgacgatga aaaaacagaa gaagatgtag taagtttgca agcacaagta
     6901 aaagcagcac gggctaaacg ggacggattg aaagagcaag tggcaaatat ggaagccgaa
     6961 caagtcttaa acgtcaaaaa agaaccatta gataaaaaag atgaaaactt gaaaaacaag
     7021 tttatcaaag actttaaagc gatggtcaat ggtgatcctg ctattatggc tactttgaca
     7081 tctaatacgg atgaatctgg taatgctatc ggattgacta ttcctgtaga tgtgcaaacg
     7141 actattcata ctttggttcg tcagtttgac tctttgcaag aatacgtaaa cgttgaaaaa
     7201 gtgaccacta ctagcggttc tcgggtttat gaaaaatggt ctgatattaa cccactgacc
     7261 gctttggata ctgaagacgg tgaaatccca gcaaatgatg atcctgcact ttacttgatc
     7321 aaatacttga tcaaacgcta cgcaggtatt tctacagtaa ctaacagctt gctaaaagat
     7381 actgccgaaa acattttggc atggttgtct aactggatcg cgaaaaaagt agttgttact
     7441 cgcaatacaa aaatcttggc agctattgat ggaatcaaag cagcgcaaaa gaaagatgtt
     7501 acagatgttg atgggattaa agatatcata aacgtccaac ttgatccagc tatcgaagct
     7561 acatctatgt ttattacaaa ccaagatggc ttcaatgttt tagataaagt gaaacgtgct
     7621 gatggatctt acttgttaca aaaagacgta gcttctgcaa ctggatatac cttcttaggt
     7681 aaaccaatca agaaaattgc ttctcgtttc ttgccaaaca aaggtacgca agctactcct
     7741 aaatatccac tgtatattgg tgatctgaaa gaagctgtta cattgtatga tcgcgaaaac
     7801 atgagcttgc tgacaacgaa tattggcggg ggggcttttg aaacagacac caccaaagta
     7861 cgggttatcg atcgatttga tgtgcagtta gtcgatgatg aagcagttgt tttagctaca
     7921 tttacatcta tcgcaaacga agtgccagta acaaacggtt aaggagctga tttcttatga
     7981 ttcttgatcc taaaatggat ttagacgaaa tcaaaaacgc actaaagatt gataccgatg
     8041 atgacgatgt ggaagtaaga cgtgcggtgc aagctgcaat tgcatacatt agaggggcta
     8101 tcgggaatga taagccctct ttttataagc aagaaaatga cacggttgat ctgattaatt
     8161 tagctattct gcaattagca gatcattatt acaacgctcg atcagcaacc gtgagtggga
     8221 acttgcgaga gtacgattta ggttttacaa gcctaatctt gcaactcaaa gcaagatatt
     8281 tgctttttgt ggaggagtag cgtatgcccc ttattcaaac aggaaattta aatcaacgca
     8341 tcaagtttgt ccgagatacg acggtcaagg acgaggacgg gcaagttgtc cggacttcta
     8401 caaccattct tacttgctgg gcaagtgtgc agacacaacg cctgaacgat attaagacgt
     8461 ccattggtac ggctttggaa ggaacactga cgttcattat ccgctatcaa caaaaatcag
     8521 agctaaccaa tgatatgaaa gtgcgttgga atggaaaaac gtttgaaatc attacgatta
     8581 cgaaaggcga gtttgcgaag gacttcacga caatcattgc aaaagaggtt tcaaaatgag
     8641 tgtagaagtc gatgcaaccg aagtgtacaa agcgcttagg gaagtaaaag caaacgttca
     8701 acgagtggaa agcccagcac ttagaaaagc tggggagtac gctcaagaaa agttacaaca
     8761 aaacacacct tactgggatg gaacgaagtc aaacggtaaa cgtggttcgt atatgcaaga
     8821 acatgctaag gaccatgtgg ttacaagctc ggtaaaaaac ggattgatag aagtcggcta
     8881 tgataaagat gtttcttggc ggatgcactt tatcgagttc ggaacaatca aacaacgtcc
     8941 aaaaggtttc gtacaaaaaa cacaaaagca aatcgaaaaa caagtaacac aaatcattgc
     9001 tgacgaagta aaaaggaggc taggactttg aaaacggcag tatcacaagt ctattcaatt
     9061 ctgaatagca atgaaaaaac aaagaacatt gatttttaca ccaatagtgt tccggaatcg
     9121 gctcaaacag tacctagcct tccagttggc agaattacag agatattcgg caactatgaa
     9181 gatttcgcaa gcaacaatcc tttgaccatt caatttaacg tacaggtaga tgtatgggta
     9241 tcgaccttaa aagaggttga tgccttttat tttgcccttg atgagattat gagggggaat
     9301 ggttggcaat gcgcatacac ggaacaaaca gatgatgagg atttagaagg tgcaaagcgg
     9361 attatcaaac gatatgtagc aaatatttca ctaaattaaa aggagagaaa atagatggca
     9421 acagtaggat ttgagagcgt catttttggc gtaaaaacag gtgcaggcgg cactctaaaa
     9481 gaattagtag cagataagtc gaaaggcgga gcgatcgaag ctaaaattac tggattaggc
     9541 gcaacttcta acacaacata cgcttcaaac gtaccattct tcattgcaag taaaggggtt
     9601 tcgtcgccaa aagttacgct tgacgtggca gacttaatgg ataacggcat ttacagcgaa
     9661 atcattggcg ctaaaaccgt ggatggtgta aatgtaattg gttcagaaac tgaagcgcct
     9721 tacgtgtcgg tagtcatggt tacagcgaac aaagaaggaa aacgcttatt catgggattg
     9781 gcaaaaggaa aattcagtca tccagatatc gacatgaaaa cagctgaaga caaaggagtt
     9841 gaattgcaaa ccgattctat cgaaggggaa ttcatttctg atgaacgtgg ctatgtatac
     9901 ttaacagccg tagaatcaga agaaatgacc ttacaaaaat tcaaggactt ggtaaataac
     9961 aaagcggggg agtagttaac cctgcatcta caccaacgac agatacaggg acaccaaaag
    10021 aaccagaacc aaaaattgat acacaaggtt agccattttt tggctagcct tatttttttg
    10081 taaaaacaag gaggaaaaca aatgattgaa ttgcaattga aacttgacgg aaagaaaaaa
    10141 acattcaaac aacaagatat ttccgcacgt gcaatgcgtg agtgtatcaa attttacgag
    10201 aaagcggaaa aagcagacct aactgattta gaagcaattg attcaatgat tgcaattaca
    10261 gcagatattt tccaagatcc agcagttaca tttgatgcta ttttagacgg tttgactgcg
    10321 agcgagttag taccggcatt agaaagtgtt tttgaacaaa tcaatgaact gggaaacaat
    10381 gaaaaaaagc agacggcgag caaaaagaga taagtttttc tgaagctagg aaagcaatgg
    10441 atcaaatcta caaagattta atcgaatcag gttggacgat gagagatgtg gacgaagccg
    10501 actatcatta tttgttacac ctttttggag aagtggagag tggcgaagaa tatgtagatg
    10561 gtgctgattt catcaaacaa tttttatcgg ctgaagactt ggtaaaactt gaggaaggag
    10621 gtaaataatg gcaggaaaag gacaaccggc aggaaatatc aagctaggga ttagtttaga
    10681 tagcactaat tttggtaaca cgctggacga aatcaatgcg aaagtcaaac aagctgagtc
    10741 gaatatgcgt gccaatctaa aggcttatga ttcagcagga cgttcatacg aagcacttag
    10801 tcaaaagacg aaagacttgt ctacggttat ggaagggcaa aacgccaaag taagagaatt
    10861 aacaaagcgc cgtgatgaag cgattagcaa gtatggcgag gaatcgaaac aagttgctaa
    10921 ccttaacaca cagataaaca atgctaccgc aaaatataat gcttacagtc gccagttgaa
    10981 tgacacaaaa aaagaattgg tgtattccaa aacagccgtc aatgatttat ctaacgaaat
    11041 caaagaaaat gaacgacaaa tgaacgccga agtaaaagcg ttgaaagccg ctggtgatga
    11101 atctggtgcg tttgaagcaa aacaaaaagg gctagccaaa caaacggaat tatccgagaa
    11161 agctatcgaa gaacagcgca aagttgtgaa actgatggct gatgagtttg gcgattcagc
    11221 aaatgaaacc gaagatgcaa aaagggcatt agaaaagtta gaacgacaaa gccaaatatc
    11281 tagcaggcaa ttagaagcac tcaaaagctc cagcgatcaa tcaggaaaaa aaatagaaga
    11341 ttttggcgac aagtccacaa ggtcagctag gaaactggat gggctaaaag acaaattagg
    11401 ctcgctaaaa agcgcatttt cgtttggtgc agttgctgga ttagcgcata acgctattag
    11461 cagtgtagta agtggcgtgc aaggcttggt tggcgaagca gtaaacgcat cggattcatt
    11521 gatgaagttt tccaaaacca tggagtttgc taactttggg aagtcacaga tagaaagctc
    11581 gaaaaaagaa atgaaagact acgccgataa gacggtttat ggtttagaag aaattctgaa
    11641 cacaaccgca caattggcat ctaacgggat tcctaactat acagaactaa ccaaggcggc
    11701 aggtaacttg aatgccgttg caggcggttc tagtgataca ttcaaatccg ttgccatgat
    11761 gctaacgcag acggcaggag ctgggaaact aacaactgaa aactggaatc aattagcaga
    11821 tgcgataccg ggcgcttcag ggctgttaca agacgctatg ttgaaaaacg gagcttatac
    11881 aggaaacttc cgtgatgcaa tggcgcaagg tcaaatcact tctgatgagt ttaaccaagc
    11941 gattgtacag ttaggtatga atgacggagc agttaaagca gctacttcca cagatacatt
    12001 gagtggttct tgggaacaga tgaaatctac tgtaataaat gggttacaaa gtatcataga
    12061 caaagtaggc gttgaaaata tcactggttt catcaacaga gtaacaaaag ggattgaaaa
    12121 ttctattcct aaaattactc aatttatagg ttggttgaga gatattggaa cgtggatcgt
    12181 tgaaaataga gagccactaa catggattgt cggaatcata ggcggaatta cattagcagt
    12241 aaaagcattg aacgtagcaa gtatgttgct ggcaattact ggcggaacat tggcagcccc
    12301 ttttgtggcg attggtgtag cattaggcgc actagcaggt gctttggtga tagcttatac
    12361 aaaatctgaa acatttagaa atatagtcaa tgcggctttt acagctgtga aaaacgtagt
    12421 tatgagcgtt atcaataatt tggtggaata ctacaaaatg ttgtggagcg tgttgcagtg
    12481 gctttgggaa aaaataaaag aatgggcttc atggattggc aataaattca ttgaaatgaa
    12541 gaacagcgtt gtgaatacgg ttcagaacct atggaatggt gtgaaaaact tcttcagtaa
    12601 tggcgttgga gacacttgga ataaggtagt tggctgggta aaaaacattt tcaacaaagc
    12661 aactgaattg aagaacaaag tttctgatgc aattggtaac ctgtggaacg gtatcaaaga
    12721 cacattccgt agaggcattg atacggtatt caattggttt tcagaactac cgacgaagat
    12781 gaagaatgcc attattggcg gtaaaaatgc cattgttgat gcgttcaaaa gtattttcaa
    12841 cgcagcactt aaagcgatag gtaaaccagt taacgcaatc atccatggag cttcatgggt
    12901 actagaaaaa ctgggtgctg acaaactcga agaatggaaa gtgccacaat acgcaaaagg
    12961 aacaccaaac ggaggtcatc cgggcgggcc tatgatggta aatgacggta gaggtgctga
    13021 agcggtaatc acacctaacg gacaagcatt tatcccacga gggcgaaatg tagtgttgaa
    13081 tgcaccaaaa ggcacacacg ttctaacagc tgaagaaaca gcttatatga ctggaaacaa
    13141 agcaccaaga tatagatacg ccaaaggtac aggctttttc ggaaatctat ggaacaacgt
    13201 caaaggattt gctggagatg ttggaaacaa gctgaaagat gtagtcggcg atgtatggga
    13261 ttttgtaaca gacccgggag cgttggctag gaaagtgtta aatggtcttg gcgtactgga
    13321 agggcttgtc aaatatcctt tagatgttgg taaaggtatt ctaagcaagg ctaccgaagc
    13381 attgacgaac aaaatcacag aactattcag cagtggcagt ttagacactt caatgggcat
    13441 gcaaggcgtt tacaaatact tggcggacgt tgcagttgca gtaatgaaga agtttccagg
    13501 ctttgtggca actagtgggt atagaccagg tgacccctat tcgcatggta aacgtaatgc
    13561 cattgatatt gcactacctg gtgtcacagg aggctcgcca cgctacacag aagcagccaa
    13621 ttacgcattt gagaagtttg caaacaaaat cggctatgtt atcacaaatg gcaaggttcg
    13681 tgaccgttca ggacaatcag gtacaggtat tcacaatgat tggagaccat ggcccgatgg
    13741 agatcactat gatcatgtgc atttaaacgg tgtgaaagac ccacaaaaca ctcaaatttc
    13801 aggagatagc gtgggaggca gtggggtaga aagatggcgc aatgtagcaa ttagagcgtt
    13861 gaaaatgacc ggtcaataca gtactgcaaa cttaaatgca ttactaaatc aaatgcgtac
    13921 agagtcaaat ggtaatccta atgcagttaa caattgggat attaacgcca aaaatggaac
    13981 accatcaaaa gggttgctcc aagtgattga cccaacattc agacagtatg caatgccagg
    14041 attcaacagc aatatttttg acccactatc taacatctta gcttcaatca gatacgcact
    14101 atcaagatat ggctcactaa caaatgccta tcgtggagtt ggttacgcaa acggtggaat
    14161 tgtaaaccaa catcaaattg cggaaatcgc agaaggaaac aagccagaaa ttattattcc
    14221 tttagataag gctaaacgat caagagcgat gcaattattg tctatcgcaa tggacaaact
    14281 aggagtttct cctaaaactt atggaaatgc gacaactgtt tccagtgatt acgaaatcct
    14341 agaagccatt gaaaaacagg cggcaacaac gaatcagctg ttatcgttat tgctggcatt
    14401 tttcaaagga aatagccgaa ctgatagaga tttagcttta gatattcaga agatcttggt
    14461 taggaggatg tagatgcgaa ctgtattact gagaaataga ataaacgaag aaattgattt
    14521 gtctacggag gattattttg ctactgggtt gagtaatatg gggtttgaag taaaaaaaga
    14581 acatgtggga cagtggggga atttcagaga aagcagtgag aacgttgaaa tatctgaatt
    14641 tcagtcatct gtaatcattt ctgtgcatgg gtttcgagaa aaagaactgt acaattcact
    14701 tgtgcagttc ctatcggaag gtccgtttga actggaattt gcttttgacg gcgaaacaat
    14761 ggtaagaaga tgcagtctaa aatctttaag taagactgaa atcgatccga aaacatcgtt
    14821 actaacagac actttagaac tatatttcac atcaaactgg tattccgtaa aacgcgaaaa
    14881 attaattcag cgaccgaatg tagtgaaaac acgtggtaag gtttttccgt ataaaagatc
    14941 ttatatctac actcaaaact tgtgggagaa aaaaggtgta ttcaaattca ataataattc
    15001 tgtataccta acgaatagca aggaacggat gtccccgcta aaaattcgtg tgattgggaa
    15061 gtgttcaaat ccgtattggg aagtaatcca aaactcacaa atcatcgcaa cggatggata
    15121 cttcatagat atgactgaaa cacagactct agaagtatca agcctttttg aagatacaac
    15181 ggcgatttta aaagatattg caggggtaga atcttctgtc taccaacaac aggattatac
    15241 aaaaactaat tttgttcagg ccccgactgg agaattcagc attgtgtttc atgttggggg
    15301 ggcagacgta gagattgagt tatacgagga gcgtgatctg ttttgatttt agcagtcacg
    15361 ctttttcatc gtgatttaat gctttataac gaacatttat tctctaacag ttttgaattt
    15421 ggtgcggacg agatcaacga agaggctagt agctttacga tggataagta cgttcctgta
    15481 aaaacaggcg attttctact agcaaaatat attcctagtg gaaaatttgc atattttggt
    15541 gtaattacgt cgcaggaaga cgaaaaaatc agttgtaaaa gcttacttag tttagctgat
    15601 agtgagatac cgactgcacg tgtgtcagga gataactacg aagagcatat ccggcggttg
    15661 attgaatact atttgctgaa tgatccaacg aaacaactaa aagatatttt agacgtcaaa
    15721 gcagaaagtg cgacctctca ttcgtatcaa gctaccgata cgaataaaca caagttaagt
    15781 gcgtatattc tcaatggttt caaaaaatac aacgtaaaat ggtatttcaa gggaattcaa
    15841 aacagaaaaa tctatacagg catacgtgct gtaaatgaat caatttacat taaagacaat
    15901 tcttctgaat ttagcgattg ggatgtgttt gttcaggcgc cgggcgctgg aaacgagaac
    15961 aagctattaa tcgttgataa agcaatgaaa gatatagaga aaccgataat actgtcaaca
    16021 tggtatttgg acgaagaaaa caatttgaca caagatggat cgaaagaaaa tatcacgaaa
    16081 ccaactgtca atttggttaa tatctacgat caaaccgcag aagataaagc atcctacgaa
    16141 gatgtggcaa aatcagagtt gaaaggcaat acgtattcgc atgaaatcaa agtgaacgtt
    16201 gtaagaaatg caaaaaattt gaatgtcgaa acgattgaaa cagggatgtt tgccacgatt
    16261 tcttataaag gaaagatata caagtcggtt ttgacagctt ggcgaatatc aagtgataag
    16321 gagtttgtgg aattgacttt cggaaacata agaagtcgtt ttatggatta ttttgaagat
    16381 aatgggggat aaaaaatggt tagcaatgtg gatggatatc aatttgaaaa cgtgaaagta
    16441 agcgcagaaa atgatgctag actttatcac gttttatata atcggaaaaa tcaggttatt
    16501 gatggttacg atcagtctat gaatttatct tcaagcggat taacagtaaa agttgctgca
    16561 ggagcagcga ttattcaagg tcgtatggtc gttgttcgac aagaagaaag tataacagtt
    16621 ccagcaaact caagtggtta tatagcatta acagtggatt tgacacaaga agttatacct
    16681 ggatctattc ttccagaatc ggaagaatat gaatggacta ataatcaagt caagctagaa
    16741 tttataacaa aagttataaa aggtaattta aacaatggtg ataaggtcta taatttacca
    16801 ctatgctcag ttacttctac tggatcaact gtttcaatct caaagatatc ggatagttac
    16861 gagctgactc tctctaaagg agaaattttg tggagtggga ctgcgttaat gcatgatact
    16921 caaactgtcc aaccttcaaa aaaaatgtgg cagacagttt caggtttttt attagtatgg
    16981 ctcccatacg aaaacgggca agcaattggg gatagatatg taactacgcc tttttataaa
    17041 gagcgtgtaa cttttactaa tgttttagga gaaattgttt caggatttga tgactatcac
    17101 aaaaagtggt ttagtaaacg aataaactat aattcgaata ctaatatatt tacaggtgca
    17161 gcaagcaatg caagtggaga taacgcaaat atggtgctta ggtatatagt ttctttttag
    17221 ttaggaggta tggaagagtg gcaaatttag aaattaaatt atctgcaaat aagagacagc
    17281 cttatctacg tcaccgtgtt gttggtagag ttggtgatgg ggggctcaca acaatcaatg
    17341 tacaacttct tgaggaagat gaaattacac cttttgtaat taatctaaac ggtactttga
    17401 aatttgtggg cgaagtttca aacggtaact ataccgaggg agaaccagaa ataatcgatt
    17461 cgactaatgg gttaattagt tacacgttca ctaagtcaaa tttcagcacg agtcatcaat
    17521 tcaaacaagc atattttgaa tatgtagatc ctaacggcaa aaaagtaact tttcagaact
    17581 tcatcataga cgtgttagaa cgagtagata ttaattcgga gcaagcgaaa tactatattt
    17641 cttcattgga aaaattacag agtgaaatgc aaaccacttt caatcagttc attagtgata
    17701 aacaggtcca atacgatcaa atctactcga aatataacga attagtaaga ttgataaatg
    17761 aatcagataa gcaagtaaat gatcgtattg ataagacaaa tgatcgtatt gatcaaacca
    17821 atcagcaaat cggcgatctc ggcaagctga aaaagatgta cagtaacagc atcgacttcg
    17881 gggactatga ttatagtggg aatccgaacc ttagcgctaa acttaatgct tcaagttttt
    17941 ctttaggtac tggcgcaaca gtcgcggatg ataatgatga gattgtgttt accttagatg
    18001 gcacaaatca actatcgaag tacgctagca aagcgcaagt gccggtagca gaagggaaac
    18061 aatatacgat tagttgtgaa attatgttag aagatgggtt cactggcgac ccttcaggaa
    18121 tacgtctgca acacgcatat ttgccaggcg gggttgttgt tttacagaca gagacagtac
    18181 ctaaaaatga attgaatacg tggcagacgc ttatcggaac gcgaacggta aaatacacct
    18241 ctgacgtacc aaatgaatgg taccctttgt ttagagacat ccgaacactt aaaccgaccg
    18301 gtaaagttag actaagaaaa attaagattg aagaaggctc aacagccacc ccatatcagc
    18361 caaacttact tgatgaccct tactggctag gtaaagcgcc tttgggtgag aatattgcta
    18421 ataagtctgt tacgtttcca atcaaatcta gcgcctacag cctataccaa gctaacatgg
    18481 aagaagaatt tgttttaggt caaacctata caattaccat gaaggcaacc aaaccaccta
    18541 tccaaacgtt cattgtttac aacgaggata gtggagatta tagatatggt aacttagagc
    18601 cagtagaagg gttagttgat acatggggtc tgacttttac accgcagaag gttggagtta
    18661 actaccccaa acggttaact attatccagt atccccaatc aacaacaggt gcatgtcaaa
    18721 ttgactggct caagatcgaa aaaggcaata ctcgaacccc gaatattagt gagtataaat
    18781 atcgtggtac tggtatgcgt gattcaaaca atccaaaaga ttatgtttgg gatctagcac
    18841 cagaatatgt cgaagataat ttggccacag atattaaaat ttctgaaatt actggtaaag
    18901 caaacaatta taccgatggg aaagtatcgg agattaattc gcagttgact gcttcaatta
    18961 atgaagtaga caccacagct aaggatgctc aaacaaaagc gaatgctaat gcgactgcta
    19021 tagatgaatt agacaataag atcgatgaac gcattaatga tacagctact accacattaa
    19081 cagttacaaa cgggaatacc ggatcagcaa agctttatcg tgaaggaaaa acagtttcta
    19141 tatattttgt ggctttaaac ggaaaaagca gtggtggaaa tgattcaacg atactaacaa
    19201 ttccagaagg ctatcggcca ccaattagtt ttgagcaact ggttggctcg atagaccgtt
    19261 ctactttgaa cagtgctcag ttatctattg gtgcagatgg agccattaaa tggcgaagaa
    19321 actcaagtta tggatcggat tatacctttg caattactta cacgatttag aaaagcgtga
    19381 atcgatatga aggcagcata tagaccaatt gaaccttacg gattcgagca aatcattgtg
    19441 aatgatgaag aacatttacc ggaagaatgc acagaagtcg aaccaccgat tctaaattgg
    19501 aaaccgaaat tcaattactg ggagggaaat aaatgaaaaa catttggaaa tatggacgta
    19561 ctggcggaga gtacgcagga aaagtattgg acgacatgct tgtatccgtt ccttacacag
    19621 atcagccacc gcttgaagga attcgtgctg atggcgaacc gttaacaatc gctgatcaaa
    19681 tgtttgatcc taaattgaat caatggatta ttttagcgaa cgcactagat cacaacgatt
    19741 taaacaatct caaagcaatg tatgagtcgt tagaaaatga gaacggcgat ttaaaacaga
    19801 tcaatgccaa actcatgcta agcgatgtag caattaaaca ggaaaatact gcattgaaag
    19861 aaaaagcgga tagtttagca caaatcaatt caaaaatgat gcttgcttcg ttacaaaata
    19921 gcaaagacat ttcagaaatt aaagagcaac taaatccagc ttcaaaggga ggtgagtagt
    19981 atgtttagtt ttagcgatgt gaaaatgatg tatgattggg gctgttttac tgacgatcaa
    20041 gttcgactat tcgttccact atgcattaca gacgaagaag cagataaaat cattagcaaa
    20101 gaagaaagcg catcttaagt gatgcgtttt tatttaaggt aaaggagttg tcacatgatc
    20161 aatttagggg aatggggaac aatcgcagga tcaattactg cgattgtttc tttgatttta
    20221 ttagtaataa gaccagtcac tgcatctttc tcgaagatta ctgaaactct ttcaaaagta
    20281 agtcacaatt tagatttgct gactaaagat ttagaatcga gcaaatcaga tcgattgatg
    20341 attcatgaag aactaaagaa acacgatgaa agattagata cacatgcaga aaaattggta
    20401 gaacacacac aacaaattaa aactttattt agagaaaggt ctcggtaaaa aagaaaggag
    20461 ttaagaagaa atgattttac ccgataagta ttatcaagtc attaaatgga cagttttaac
    20521 agtattgcca gctttatctg tattagtagc cacattaggc aaagcatatg gatggaatgg
    20581 aacagatatg gcagttctga ctatcaatgc agcagcaaca tttttaggtg ttatcactgg
    20641 tgtgtcggct tataatttga aaaaatagga ggaaacaaat gaaaaagaaa attactatta
    20701 ctgcgatgag cctattaacg gctctttttt tattgccgat taatgggttt gcctatacga
    20761 ttaacaatga atttaattta ggtgtaaacg aaggtagttc tcaagtagca aataatcagt
    20821 acattttact gcatgaaacg gctaatgaaa cagcaacagg acgcaatgaa gcgcagtata
    20881 tgcaacgttc atggactagc gcttatactg cttatattgt gggagacggc ggaattgttt
    20941 atcaagtcgg tcaacctggt tatgtacagt acggtgctgg ttcgtatgct aatgcaaata
    21001 gtcctgtgca gattgagtta caacacacac atgataaagc aacgtttgag aaaaactaca
    21061 aggcatacgt tgaattagct agagattcag caatgaaata tggtattcca cttacactag
    21121 acacaccgta taatcaacca gggatcaaat cgcatttatg ggtaacacaa aatatttggg
    21181 gcgatcatac agatccttac ggttatcttt ctgaaatggg cgtaagtaaa gaaaaattag
    21241 cctatgattt ggctcatgga tttaccgatg aaaatccgac aacttcagat gataaaccag
    21301 tcattgatcc aactcgagca ggtgctgcaa atcctaccct gacagatgga acaaattacg
    21361 cccacattga tcagttcgga gaaatcgaaa acgcaaactt acatgtggct ggttggcaca
    21421 tcgctaacta taaatacgag tatatcttca ttatggatta caatactggg aaagaattag
    21481 ctcgagtaaa agctgatgga atatatagac cagatgtaaa tcaagcttat aatactttag
    21541 gaaatgttgg ctatcatgta tcttttaaca tgcgcaactt ccctaataag aaagtctatg
    21601 taatgatgcg ggcaacgaat gatccagaag ggaacactaa aggcggagca caagattttc
    21661 atgataaacg ctggtattta aatattccgc aacgataaaa atagcccctc gtttttgagg
    21721 ggaagtacat aatgcaaatt tgtccataac ttattgatta tcttgtaaaa atgcggtaat
    21781 ttaatagtta gtgagaagag gtgcttaatt atagttaaaa aagaggaaat agaagcagca
    21841 tgtaaccaag caaatgttag gtttattgga gaagctttat ctggatatcc taagtataaa
    21901 tacttacaca actggttaca aagtaaaagt ttttatctta ataaagaaag atgcatgcct
    21961 aataaaaatt atagggttta taaaagagga actattgtat atgttgattt tggggtgaac
    22021 gtgggttatg agttatctgg taatcatttt gctattgttt taaataataa agataattcg
    22081 aaaaatggtg ttgtatgtgt agtaccaatc tcttcaaaaa agaaaagtaa ttatgtatct
    22141 gtaggaaaaa tattagaagt tgctagtatt aaacaattta taagccaagc tgataaatta
    22201 aaagagaaac ttaggataat tattttactt tcattaaata gaggcatcat tgatgaaagc
    22261 gatttgaata gttttattaa agatattgaa gctaaaggtg aaaagctttc agtggaagaa
    22321 gtagaaaaac gagctgtaaa acataatttg gtttgcgaga caagggaaca agtaattaaa
    22381 gaaataaaat cattatctga agatatggta aaatatcaga aggtgtttaa ttgctacaag
    22441 aaatatacac aagaatccta tgtaatgcca cttaatatcc aaacgattag taaaaataga
    22501 attagaagaa ttaacaagtt tgatccttct ggaaatatga aggcacctgg aaatgtcctc
    22561 aatgaagtag atgaaagttt aaaaaaatat tttacaaagt aaacgatatt tttgttgaca
    22621 tttttctgtt cgttagttac catgtagtta tagaaggacg taagcgccta tttttaatgt
    22681 acacatcggg acgtatgcgc ccacatagaa acatagttga gagacatcta tatggtgtct
    22741 ctttttttat taatatgcaa aaaagtctag ttttttcaat aatatttatg taataataaa
    22801 ttagccacca caacaaagaa tgaaatccat tattatctag tctagtccat tctttttgtt
    22861 tgcagtattt gtgatggctt tccgtaccct tagctcagtt ggtcagagca gacggctcat
    22921 aaccgtccgg tcgtacttct cgtaatccag gaaggccgtc agctccgtcc gcagtagcgt
    22981 gtttacggcc agctccagat gggagcggaa taactcgttc aaatcacctt tggtggctag
    23041 agtctgcagg agttctgtaa taaaatcgtt catagggaag tcctcttttc tgtgaatggg
    23101 tgtggtaact ttattctaca gaagggactt ccttttttct atttacacaa aatattttat
    23161 actctcaaat gtttgtatgc agttacatct gactttattc gaattaagga aaagatttat
    23221 aaaaataaaa taaaaaaata tatatttaaa ttgacttatt gtgaaatcat taactataat
    23281 tagttcagtt tatgttaagg agaatgatga tatgaataag aaaattgtag taagtggatt
    23341 aattataggt ggattagtgt tagcagggat cgcagggaaa aactttgccg atgacatttt
    23401 gttatggggt ggagaagata atattgaaca aatcaatcat aatttggagt cattagataa
    23461 agcactagaa aataaagagc aaaatattag tagtttaaat tcacgattat cttcaaataa
    23521 tcaagaactt gagcaagcaa aggctaacgt tgaagaatat aaacaaaaag tgttgacttt
    23581 ggaaaatgat aaaaatcaat taactataga gaaaacaaat ttagagaatc aattatcagg
    23641 aaaaaatgct gaattgcaag gtaaacaaaa tgaaatcaat gctaaaattg atgaaataaa
    23701 tcaaaaaata gctgaaataa atcaattaaa cactgattgg aataatcgtt tacaaacaga
    23761 acaaaataaa tttaatgatg ctcaaaatac tgttaatagt ttaaatcaac aaattaatca
    23821 attgtcaaat gaaaaaaata ctattaaaaa ccaattgcaa agtactgaac aaaaactaga
    23881 aaaagcgcaa tcagaaaaca gtaatttgaa taattatatt gaaaaactgg aaaaagccaa
    23941 aagcgatgta gaagacacag caaacagatc acaagaaatc gtgactgaac atacaggaaa
    24001 atagatgcat tttttagttt aaatttcttt aacaaaatag tattttgttg ctaaataatt
    24061 tttatttgaa tattctacaa tgatatctcc cctcaaaatg gaggggaagt acatactgtg
    24121 ataatctaca ggtttattga ctgattagga tgttattaaa aaataaaata ctaaaatgca
    24181 tacccgaatt tcatacccga aaaataaaaa atgccaccac gtacactctt ttcgaaacta
    24241 cctaagtagc caagaatgta ctggtgacgt gggtttggca cccaatgggc cgtgaggggc
    24301 tcgaacccgc gacccgctga ttaagagtca gctgctctac caactgagct aacggcccgt
    24361 acaaaaagta cttaataata gtagcactaa gaattttaaa atgcaagact acatttaaag
    24421 aaaatcagaa aaatctttac ttccttatag gaaatcattc atagattatt cacatttgct
    24481 cgctattata taagcatacc aacaaacaaa gttcttgcaa gagaacaaac cctaacactt
    24541 ttttcatttt ttactccttt cccgccgtct ccccggcggg tctcctgttc ttaacgataa
    24601 aagacaagaa ggcttagcct tcttgttttt tgttttcatg gaatcccgct aatcaaatca
    24661 cctagttaaa gcaactagat tgaatgttta tcataagtct gatatcatgt ttatgaaatg
    24721 aaaaaaggag cggttatgat ggaaaaaatg cgtcagcaat taatggagtg ggggaaggaa
    24781 ctagaaacat tccgtctgcc tcactgggaa gaactccctg acttagaact ttatatggac
    24841 caagtcagaa cgttagttga tcgttatctt tcacctgtga tacaaggaga aaagcatcca
    24901 ttgcttacct cttctatggt aaacaattac gttaaactag gtttgatacc agcaccagtt
    24961 aaaaaaagat ataacaaaga acacgtagca tttttacttg caatcactac gttgaaacaa
    25021 gtattgacta ttcctgaaat caaagaaggg atattgttcc aaggaaaaac agtaggaatt
    25081 cgtgaagcct ataatctatt ttgcgatgaa caagaagcag ctgtgtggat ggtcagccaa
    25141 ttagcacaag gtaaatctca tccgcaaaaa ttcgaccagg caacccctgt agaatatatt
    25201 gctgtaagag cggcaacttt atcgtttgca atgaaacttc ttgcggaaaa aacgattgtt
    25261 ctagaaaccg aatatttaaa agaggagaaa acaaatgaaa aacaataaaa tcgcaatttt
    25321 agtagattca ggtacggatg tgccagaaga attgataaaa aaatacgaga tgtatatgat
    25381 cccattgaaa attatttata aagatcaggt atatacagat aaaattgaca ttacctctga
    25441 agagatctat caacgtttgc cagaggaaat ccctggtaca tctttgccgg atggtgagtc
    25501 gatcacagaa atctttgagc aaatcaaaaa agacggttat gaaaaggtac ttgcggtaac
    25561 aatttccagt ggattgagcg ggacatataa tgtggttcgt ttaattgcac aacagcaaga
    25621 agaattggaa gcatatgtac tagatacgaa aaatattggt attggtgcag gtttttctgc
    25681 gatccaagca gcaaaatggc tggaagaagg agtagaatgg gatcaactga tcagtaatct
    25741 gaatgagtta gtcgaaagaa caaaagtttt ctttaatgtt gcgacattgg aatatcttca
    25801 aaaaggtgga cggatcggtt tagttgcttc tattgtagga accgctttaa agcttaaccc
    25861 aatcatctca tgtaatgaag aagggattta ttacacagta ggaaaagcta ggggacgtaa
    25921 aaaaagtttg gataaaacgg ttgcttacgt caaagaaaaa atcggtaatg caaaggtctt
    25981 caacttagct gttgcccatg gggatgcgaa agaagaagca attgaaatgg aagcgagact
    26041 taaaagagag ttcccacaag caaaccaaat ctactttggc cagatttctc cggcattagt
    26101 agtccacact ggtccgggat tattgggtgt cggtgtacaa gttttagaag gataaatcaa
    26161 aaaaaggaaa cactgaggtg aaaatcagtg ttttcctttt ttattgaagt tagaacaaaa
    26221 aataataaag tattttgcac tttcatttta attttctctt ttgttttata ggcaaagatg
    26281 aaacaaaagc agaaatacgg tataatgaga aggtatgaca ataatatata agaggtggct
    26341 ctttatgaaa aaagttttag ttgtcgatga tgagaagccg atttcggata tcgtcaaatt
    26401 caatttagcc aaagaaggct atgatgttta tactgcatat gatggggaag aagccttgga
    26461 aaaagttgct gaggtagaac ctgatttgat tttacttgat ttgatgctac ctaaaatgga
    26521 cggcttagaa gttgctcgtg aggtacgcaa aacttatgat atgccgatca ttatggtaac
    26581 agccaaagat tctgaaattg ataaagtcct aggcttagag ttaggcgccg atgattacgt
    26641 gaccaagcca ttttccaacc gagaattagt tgcacgtgtc aaagcaaatc ttcgtcgcgg
    26701 tgccacggct gcgaaggaac cagaagaggc agcacctgcg gaattgacga ttggtgattt
    26761 gacgatccat ccagaagctt acatggtgac gaaacgagga gaaacaatcg agttaacaca
    26821 tcgtgaattc gaactactct tttacttggc gaagcacctt ggacaagtga tgacaagaga
    26881 acatttatta caaacggttt ggggttatga ttattttgga gatgtacgaa cagtggatgt
    26941 aactgtacgt cgtttgcgtg aaaagatcga agataatcca agccatccaa attatttagt
    27001 gacacgtcgt ggtgtcggtt actatttgcg taacccagaa caggagtaat tggatgaaaa
    27061 gaaaagtccg cttttttcga tcagtcaatt ttaagatcgc tattaccttt attctgattt
    27121 tattgatctc gatcgaaatt attggtgcct actttatacg tggtttagaa cgttccacga
    27181 tcaatacatt tattaaagat atgaatcaaa cggtggaatc attagcaaca acaatcagcc
    27241 cggaattgaa tcgaaaagat aatgcagatg atgaagaggt aaatgcgaat atcaaaagat
    27301 ttattgaaaa cagtgcgact agcgatatca tcgaaatacg ggtcgttgac gaaaaaggga
    27361 tcatccgagg aacaaccgat gtcaatgaac aaagcgctgt gggaaagaaa aatgattatg
    27421 ttgatatcaa tgatttcacg actaaacgtt atgttgcttt agataacgac gataaacggg
    27481 tgaatatcaa tgttcaaccg atcctgtccc cgaccggcga tgccgttatc ggggctcttt
    27541 acgtcaaaag taatatcgaa caaaaatatt cggaaatcaa taacacagca gtcattttct
    27601 ttactgcctc actaattgcg gcatttattt caatgatcgt atctgtttta gttgctcgtt
    27661 cgattacaca gccgatcggt gaaatgagag aacaagccat tcgaatcgct agaggagact
    27721 acagtcggaa agtcaaagtg catggacaag atgaacttgg acagctggct gataccttca
    27781 atcagttagc tgaacgtatt gaagaagctc aagatacaat ggaagctgaa cgcaatcgtt
    27841 tggatagcgt gctatcccat atgacagacg gcgtcattgc gacagatcgc aggggtaaag
    27901 tcattacgat caatgatatg gctgtttcct tgctagatat caaaaacgag gaagcaatcg
    27961 gacaatcgat tttgacactt ttagatatcg aagaagaata tacgttgcgg aaattattag
    28021 aagatccaaa cgaaatggta cttgaccgtt caaccagtac attggaaagt gatcagatga
    28081 tcttgcgagt agacttttct atgatccgtc gcgaatcagg ttttatcagc ggattagtag
    28141 ctgtcttgca tgatgtgaca gaacaagaaa aaacagaacg ggaacgtcgt gaattcgtct
    28201 ctaatgtttc ccatgaactt cgtacaccgt tgacaagtat gcgtagttat atcgaagcat
    28261 tgagcgaagg tgcgtggaaa gacgaggaag tagcaccaaa ttttttgaag gtaacgctag
    28321 atgaaacaga ccgaatgatt cgaatgatca atgacctctt aaatctatct cgcatggata
    28381 caggtaatac ccagcttcaa ttagaatatg tcaatttcaa cgagttagtg aattttgttc
    28441 ttgaccggtt tgatatgatg gtgggaaatc aggaaaaaaa ttataagatc cgtcgtgaat
    28501 ttacgcaaag agatctttgg gtcgaagtag atacagataa aatcatccaa gtagtcgata
    28561 atatcatgaa taacgcgatc aagtattcac cagatggggg aacgattact tgtcgcttat
    28621 tagaaacgca taataacgtg gttttaagta tcaccgatca aggattgggg attcctaaaa
    28681 aagatctgaa ccgagtgttc gagcggttct atcgagtaga taaagcacgt gcaagagcac
    28741 aaggtgggac aggacttgga ttagctattt ctagagaagt gattaaggcg catcgaggcg
    28801 ctatttgggc agagagcaag gaaggaaaag ggtcgacttt ttatatctcg ctgccatatg
    28861 aaccttatga ggaggaatgg tgggaatgag aatctctgaa aaagtcgttc gtataggact
    28921 cattttgatg gttgcgttaa gcatatactt ctcttatgca atctggctga gcccagcagg
    28981 aaaaagttca atcaacgtag atgagacaaa cactcaaatg atcgagtctc aaagcaatcg
    29041 aaaaacttcc gaagtctttc ttccgcttca tgttacatgg caccattcgg gaatgatcca
    29101 agaaaccaac agtgaaaatc tcgtgtaccg attgcaattg atcatcgata aagcaagatt
    29161 tggaaagctt tctgaagtag tttcaggaga caaatcacag tttgaacagg tgaaaaatct
    29221 gaactctggt tttgagttgg cctataatgc gccttttttg ctaagagaat acaaggaagc
    29281 atttgattta gatttagatg tgtcgaactt ggcaaaagaa agcgaaaata tgtattttac
    29341 tagaatacaa ttcgatcaag gaaataaaaa aatccgattt ttgaattatt cgaaccatct
    29401 cgtatatgaa gcaaatattt cgttgaattg gacagatatc aataaagaat taacagcaaa
    29461 agacacaaaa tggacagaaa tgacaacaga accatcgacg atcgacacgc aatatgctac
    29521 gaaacattcg atcaaactga aaaagtatag ttatatcctg tcacagcaac cctatacact
    29581 ttttagaaat gcttttttcc agaaaccaga tagcgtgaaa aataatgatg aaagcaatga
    29641 attgattttt tacggtggga atgaaacaat gtctatccat tcggaaacgc aagtagttga
    29701 ttttcgtgga gagctgccag agaaaaaaga aaatgctagt gtattttctg aaagtttccc
    29761 ctatattagc aaattaggca gttctttagg gaacctcaga tattttgacc gcagtgacaa
    29821 tacgatcgat tatcggatat tcgtggaagg cttcccggtt tttgggacag acagcagagg
    29881 acagattggt gtggaagttg gcggtgaaac gaatggttct gtgcaagtga atatccaaac
    29941 cagtttgaat acgattcaag tgccgatacc ttcagacgaa gaagtagaac ttgcaagtac
    30001 gaatgaaatc atccagcagt taaaagctaa gggagcagat agcaacaaga ttggttcagt
    30061 gatcgttggc tatacatggc aaaatgtcaa agaaacgaat cgtgtcgttg atctcttacc
    30121 tgaatggtat gtgaaatata aagataactg gtattcagct aacgaccttc tggaaaaatt
    30181 acctggattg gaggcgaatt aattggattt caaaaaaatc gaatggatat ttttcgtcgc
    30241 ttttttaggg ttgaatgtgt ttttattcag cagttatcat gacgcggtat accaagaaaa
    30301 caatgtcatt cgctctaatc agactgaatc aatccaacaa cgtctggcta gcgacgatat
    30361 cacttacacg gggaagattt cgtctgagaa caagcaaggc tattatctta gcgcggagca
    30421 gaccaatctt agcacttcat tagcgaatta tcgcaaggct ggtaaaagcg gcttgctggc
    30481 aagtggtgtg tttttagacg gtgatgtttt gacgaaatct ttatccagtt cagatgctga
    30541 aaagaacgtg atcgatccag ataaaattag tagttccttg gaagattttt tatctaacaa
    30601 agacaatatt ttattcggta atgattatgt gtacacgaaa aacttttctc atttagaaga
    30661 agaaccacta gaaatccaag caagccaaga gtatgaaggc atcccattca atgatgatac
    30721 agcaaaaatc gttctttctt tagaaaaaaa cgataatgat tatagcatca cgaaatacat
    30781 gcagacgcat ttatctgata tcgagcagct gcgcgaaaag acggaactgt atacggaaga
    30841 ggaagcaatc aatacattgt acatcaataa caaaatacca agaggatcaa aaattttatg
    30901 gcgtcagcta gcttattcct gtattctgaa agtccgggaa aaaaatgtgt atgttcctgt
    30961 ttggcatgta gcgatcgaaa caagcgataa agtggtccaa attgagagtg tcaacgcttt
    31021 cagcaatacg atcgtgacaa acaatactgt accaaaagtg gaagatcaat aaaaaagcga
    31081 gtataatgaa aaaagtttac aaagtaagaa gggaagtagg agtcaaatgg ctgaagaaaa
    31141 accagctttt aaaatcagta tcctagcaag tggaagttcg ggtaattcac tttatatcga
    31201 aagtgaaaaa aaacggcttt tggtcgatgc aggacttagc ggtaaaaaaa tcacttcctt
    31261 gatggctcaa attgatcgga caccggacca attagatgga atacttgtga cgcatgaaca
    31321 tcgagatcat atacatggcg tgggtgtctt ggctcgtaag tatcatttgg atgtttttgc
    31381 caatcagcag acttgggaag cgatggaacc actgattgga aatgtaccag tggaacaaaa
    31441 acatctattt gagatgggga aagtcagaac attcggcgat atcgatattg agagtttcgg
    31501 cgtctcccat gacgcagcgg ctccgcaatt ttatcgtttc cataaagaaa atcggtcatt
    31561 cgtcatgttg acggacaccg gatattgcag tgattatatg cgaggtgtca ttgaaaatgc
    31621 cgatggttat ttgatggaaa gtaatcatga tcttgagatg ttacgcatgg gaccttatcc
    31681 gtggaattta aaacagcgta ttttaggaga tagaggacat ctttccaacg aagacggtgc
    31741 acttgtcatg actgatgtga tcggtaatcg gaccaaacga atctatcttg gccatcttag
    31801 caaggaaaac aatatgaaag aacttgctca tttaacaatg gaaaatgttt tgaaagaaaa
    31861 agattttggc gttggacatg attttgaaat ctatgataca gatccagatg aagcaactga
    31921 gctattttcg atttaaacag ttcaatcaat aaaaatataa aaagcagaag ataaaatcaa
    31981 tcagggttgg ttttatcttc tatttttatt tttttagcat attaaaaaaa taaccgtatt
    32041 atcgactgac tacactgatg tgctgctttt catgtttctc atccagtatc tcatctacta
    32101 atgcaatcgc gtaatctgcc atgctgatcg tactgtcgcc agcagcgttt ctttgtaaat
    32161 aatcatcact taacaaataa gtacctgtac gtttgccatg tggatcaaat aaagaagctg
    32221 gactgatata agtccaatgt agatttttag attgtttcaa ttttaacaat ccttcgtaca
    32281 tattgaaagc tgttggatag aagggtgcat ttttaggtac ttggtcaatc atacgagtcg
    32341 tcttttctgt atctaagaat aacgaagagg cacccccaac gacaattaag cgtgtatcgg
    32401 ttccttcaag catctcaatc aaatgggaca aagaagtttg atgcatttct tcttttcctt
    32461 tgggagcgtt gaaagcatca acgacaacat caaaagatgc aagatcagta gttgtcaact
    32521 caaataaatc cttttgtata aacggaacat ctgatggcaa tttttctgga tgacgcacga
    32581 ttgccgtcac gtctaaatct ctggacaatg cttcgtctaa aataaatgaa cctgcatggc
    32641 cagtagctcc aatgagtgca attttcataa aaaacctcct tttttattcc tgtccattgt
    32701 aagcagttgc tgaatattcg tcaaagaatc cgatttttga aaagctcagt gcgcattcct
    32761 ttcatggttc gtgatcattg ggtatagtta aattaagaaa actgattgat aaaaatagaa
    32821 gttgaaggag ataagaaatg aaagagtggt tagatcgtgc ctttttacca atgataacat
    32881 ctgttgtgaa tcttagaccc ttaactgctt tgcgtaatgg aatgacttac acgatcccaa
    32941 taattattgt tggctcgctg tttttcttat ttgctcattt tcctgtggat tacatcgctc
    33001 agtggataga caaaacagga ttttctatgt attttacaca agtataccag gctacatttg
    33061 atgtgatagc cttttggatc gtttttgcca ttgcatacgc ttatgcaaaa gaagaagaca
    33121 tcgatcgctt accagtcggc ataacagcct tgtctgctta tctactgctt attcagccac
    33181 taaatactca gatagatact gtctggacag gtgctgaagg gatactagga gcagttacaa
    33241 ctgggctcat catcggatgg ggctatgcac gtttagtaag acttgcgaaa gggtggaagc
    33301 aaagagagaa gatacctgaa aatgtttggc agtcttttac ttcattgatt ccaatcattg
    33361 ttatagtcac ttttacgctt atcggttcag cggtttttca acagttgttc agaatgactg
    33421 ttgttcaagg agtatggaca tatgtccagc ttcccattca atcgttcata gacacgttgt
    33481 ttggtgtgct tttattaggt ttttttgttc cgtttttatg gttatttggt cttcatggat
    33541 ctgcgatggt caatgggttg gttagtcccg tcttgcaagc gaattcatta gcgaatgcag
    33601 aaatcttagc ctcaggacaa gagttgacgg tggcaaatgg cggacatatc gttactcagc
    33661 aatttctcga tcagttcatg acggttacgg gtgctggatt gacgttaggg gctgtatttt
    33721 ttatgacggt ttttgcgaaa tcgaagaaat acagagaatt aggaaatctt gctcttttac
    33781 cagccttttt caacatcaat gaatcaatta ttttttccac accgatcgtg atgaacccga
    33841 tgatggctgt tccttttatt ttcgcaccgg tcgtttcagg attgattacc tattccgctt
    33901 tatattttgg attcgttcct ttatttacag cagtccaagt cccttggaca acaccgccga
    33961 tcctctcggg tttcttagta ggcggagtgc ctgcagcgat tttacaaggt atcgtgttgt
    34021 caattagttt tttcatttat ttcccatttt tgaaaaaaat agattctgca aactgcaaaa
    34081 aggaaagaca gacaaaaaat gatggagcag cagaaaagat gaacgactaa aaaacaaaaa
    34141 tactcctgtc gctttagatt gggaaaatca ccaatctagg atgacaggag tatttatagt
    34201 catgtttata ctagcccttt ttggacagct aattgaacaa gaacaactaa tgtgttcatt
    34261 cctacgtgtg tgatcatcga tgtccagatc cgaccagttg ctttgtactc taggctgaag
    34321 aagaaaccta ggaaaaagta gattagcaga tgaccatcat tatgagcaaa cgcaaacagc
    34381 aaagagctga tcactgctcc gacccaaaaa tttgaatatc gactgatgat ccccagtata
    34441 ctgcggcgga atacgaactc ttccatgatg ggtccagcaa cagttgtcgc caaaataaaa
    34501 gcgggtgctt ccatgatcat ctggatgata ttttgtgtat tctcggaagg ggttgctttg
    34561 ccaaacaata cagattctaa catgacagcg atgctttgca gaatcaatgc gacaaagatt
    34621 ccaaccaatc cataaataac aatctttgga gcagaagctt tttttaccgc tgattcgatc
    34681 gctaagggtt cacgttgctt aaaataaaaa atagctagga caacggcgcc tagtatatac
    34741 atgactgcag ttgctgtgat caattcagac gtagatttta caagtcctat cgaagctagg
    34801 atcaagggtg agataaaaat tagaccatag caaaaaatgc tgaggaagct atattttttt
    34861 aaagacatgt aattcctcct tttaagggat aacaagcatg caaggcaagc ttatatcgat
    34921 tgatttcaat tatatcataa tttcaataag cagctgtcag aataaaattt cttttctttg
    34981 atttaaaagc ttgcaaaaaa gagggagaat gagtattatt aaaaatgtag gttagcactc
    35041 gaaggttaag agtgctaaca gattaaaaaa agagttatcg gagggattta tcgtgttaaa
    35101 accattaggt gatcgcgtca ttatcgaagt cgcaaaagaa gaagaaaaaa ctgtcggagg
    35161 aatcgtttta gcttctgctg ctaaagagaa accccaaact gggacagttg ttgcagtcgg
    35221 tgaaggccgt ttgttagaaa acggtgaaaa agttcctgcc gctgtaaaag caggcgacca
    35281 agttatgttc gaaaaatacg ccggtacaga agtaaaatac gaaggaaaag aatatctgat
    35341 cgtcgcagga aaagatatca tggcgattgt tgagtaaatt tggaagataa aaaaataatc
    35401 aaaaataatc aatgaggtga atcaatcatg gcaaaagaat tgaaatttgc agaagacgca
    35461 cgcgcagcta tgttacgcgg ggtagataaa ttagcagata cagtgaaagt aacattagga
    35521 cctaaaggcc gaaatgtcgt actagaaaaa tcttacggct ctccattgat cacaaatgat
    35581 ggtgtgacga tcgcaaaaga aatcgaatta gaagatcatt ttgaaaacat gggtgcaaaa
    35641 cttgtatcag aagtagcatc aaaaacaaat gatattgcag gagacggtac aacgacagca
    35701 actgttctga cacaagcaat cgttcgcgaa ggtttaaaaa acgtaactgc aggtgctaat
    35761 cctctaggca ttcgtcgcgg gatcgaattg gcaacaaaag cagcagtcga agaattgcat
    35821 aatatctcaa ctgttgttga ttcaaaagaa gccattgcac aagtagcagc tgtttcttcg
    35881 ggttctgata aagttggaca tttgattgct gatgcaatgg aaaaagttgg aaacgacggt
    35941 gtcatcacaa tcgaagaatc aaaagggatc gaaacagaac tagatgttgt agaaggtatg
    36001 cagtttgatc gtggttactt atcacaatat atggtaacag ataacgacaa aatggaagct
    36061 gttctagaaa acccatatat cttgatcaca gacaaaaaaa tctctaacat ccaagatatt
    36121 ctgccattgt tagaacaaat cttgcaacaa tcacgtccat tgttgattat tgctgacgat
    36181 gtagatggtg aagcattgcc aactcttgta ttgaacaaaa tccgtggaac attcaacgta
    36241 gtagctgtaa aagctcctgg tttcggcgat cgtcgtaaag caatgcttga agatatcgct
    36301 attttgacag gtggtacagt aattactgac gatctaggtt tagaattgaa agatgtaaca
    36361 atcgaaaact taggaaatgc ttctaaagtt gttgtagata aagacaatac aacgatcgta
    36421 gaaggttctg gagaaaaaga agcaatcgaa gctcgtgtgc aattgatcaa aaaccaaatt
    36481 gctgaaacaa cttctgattt tgaccgcgaa aaattacaag aacgcttagc taaattagca
    36541 ggtggtgtag ctgttgttaa agttggtgca gctactgaaa cagaattgaa agaattaaaa
    36601 ttacgcattg aagatgcatt gaatgctaca cgtgccgcag tagaagaagg aatggtctca
    36661 ggtggtggta cagctctagt gaacgttatc agcaaagttt ctgctgtaga agcagaagga
    36721 gacgtggcaa caggtatcaa gatcgttgtt cgtgctttag aagaaccaat tcgtcaaatc
    36781 gctgaaaacg ctggttatga aggatcagtg attgttgata aattgaagaa cgtggaacta
    36841 ggcactggtt tcaatgctgc tactggagaa tgggtaaaca tggttgaagc aggtatcgta
    36901 gacccaacta aagtaacacg ttctgccttg caaaatgccg cttccgtttc tgccttgtta
    36961 ttaacgacag aagcagtcgt tgcagataaa ccagaaccag cagcgccagc agctcctgca
    37021 atggatccat caatgatggg cggtatgatg taagaaaaag caacttgctt atctacaact
    37081 gatcattagt gaaagacaga taaaaagagg ctggaacaga agcgtttaac tccaagaaat
    37141 aagaagaaat tcacggaaat tgcttttcaa atttttgtga atttcagctt atttccgaag
    37201 gagttgcttc tgcttccgct gtttatacgt gtttagagcg tgagacaaaa gtgttcttta
    37261 cttttgtccc acgctctttt ttgtgtgtaa ttataatgaa aatagttatt tttaatagtt
    37321 ataatatttt atctattaaa agtaaatatc cattca
//
LOCUS       DANJMI010000027        35774 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000027 DANJMI010000000
VERSION     DANJMI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.027"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(35..1618)
                     /locus_tag="PWU81_001607"
     CDS             complement(35..1618)
                     /locus_tag="PWU81_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL1110312.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(2196..2339)
                     /locus_tag="PWU81_001608"
     CDS             complement(2196..2339)
                     /locus_tag="PWU81_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDL1110313.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(2562..3041)
                     /locus_tag="PWU81_001609"
     CDS             complement(2562..3041)
                     /locus_tag="PWU81_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL1110314.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(3114..3398)
                     /locus_tag="PWU81_001610"
     CDS             complement(3114..3398)
                     /locus_tag="PWU81_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110315.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(3476..3670)
                     /gene="ssrS"
                     /locus_tag="PWU81_001611"
     ncRNA           complement(3476..3670)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PWU81_001611"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3742..5028)
                     /locus_tag="PWU81_001612"
     CDS             complement(3742..5028)
                     /locus_tag="PWU81_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL1110316.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            5166..5837
                     /locus_tag="PWU81_001613"
     CDS             5166..5837
                     /locus_tag="PWU81_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDL1110317.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            5803..6291
                     /locus_tag="PWU81_001614"
     CDS             5803..6291
                     /locus_tag="PWU81_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HDL1110318.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            6304..7251
                     /gene="prmA"
                     /locus_tag="PWU81_001615"
     CDS             6304..7251
                     /gene="prmA"
                     /locus_tag="PWU81_001615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL1110319.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            7252..8016
                     /locus_tag="PWU81_001616"
     CDS             7252..8016
                     /locus_tag="PWU81_001616"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL1110320.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            8249..10462
                     /locus_tag="PWU81_001617"
     CDS             8249..10462
                     /locus_tag="PWU81_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL1110321.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            10473..10919
                     /locus_tag="PWU81_001618"
     CDS             10473..10919
                     /locus_tag="PWU81_001618"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL1110322.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            11090..12349
                     /locus_tag="PWU81_001619"
     CDS             11090..12349
                     /locus_tag="PWU81_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HDL1110323.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(12438..13385)
                     /locus_tag="PWU81_001620"
     CDS             complement(12438..13385)
                     /locus_tag="PWU81_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL1110324.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            13779..15071
                     /locus_tag="PWU81_001621"
     CDS             13779..15071
                     /locus_tag="PWU81_001621"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL1110325.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            15086..16855
                     /gene="aspS"
                     /locus_tag="PWU81_001622"
     CDS             15086..16855
                     /gene="aspS"
                     /locus_tag="PWU81_001622"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL1110326.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            16964..17839
                     /locus_tag="PWU81_001623"
     CDS             16964..17839
                     /locus_tag="PWU81_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL1110327.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            18105..19988
                     /locus_tag="PWU81_001624"
     CDS             18105..19988
                     /locus_tag="PWU81_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL1110328.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(20063..20347)
                     /locus_tag="PWU81_001625"
     CDS             complement(20063..20347)
                     /locus_tag="PWU81_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL1110329.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(20337..21071)
                     /locus_tag="PWU81_001626"
     CDS             complement(20337..21071)
                     /locus_tag="PWU81_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDL1110330.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            21200..21823
                     /locus_tag="PWU81_001627"
     CDS             21200..21823
                     /locus_tag="PWU81_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDL1110331.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            22015..23136
                     /locus_tag="PWU81_001628"
     CDS             22015..23136
                     /locus_tag="PWU81_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDL1110332.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            23143..26271
                     /locus_tag="PWU81_001629"
     CDS             23143..26271
                     /locus_tag="PWU81_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDL1110333.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVERQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            26290..29484
                     /locus_tag="PWU81_001630"
     CDS             26290..29484
                     /locus_tag="PWU81_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HDL1110334.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(29630..30337)
                     /locus_tag="PWU81_001631"
     CDS             complement(29630..30337)
                     /locus_tag="PWU81_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL1110335.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            31057..31500
                     /gene="spxA"
                     /locus_tag="PWU81_001632"
     CDS             31057..31500
                     /gene="spxA"
                     /locus_tag="PWU81_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL1110336.1"
                     /translation="MLQKSDSYHEKEWCTLLTLYTSPSCTSCRKARAWLQEHQIPFVE
                     RNIFSEPLNSSELKAILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNP
                     GLLRRPIMIDDKRLQVGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            31832..32485
                     /locus_tag="PWU81_001633"
     CDS             31832..32485
                     /locus_tag="PWU81_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDL1110337.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            32711..33709
                     /locus_tag="PWU81_001634"
     CDS             32711..33709
                     /locus_tag="PWU81_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDL1110338.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            33885..35693
                     /gene="pepF"
                     /locus_tag="PWU81_001635"
     CDS             33885..35693
                     /gene="pepF"
                     /locus_tag="PWU81_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDL1110339.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        11781 a         6347 c         7548 g        10098 t
ORIGIN      
        1 ccaacacaat ttgacgaaga gccgaaaaaa cgtcctagtc aatcagttgg ccgagcggct
       61 ggcggctgat ctgctcctga tcaataacga gtgcttcttg gaagtgttgc tgcaaaaagt
      121 agcggtcatg gctgactgct aggagcgttc cttgatagtc ttgcagcatt tcttcaattt
      181 cttctcgagc atagctgtcg aggtgattgg ttggttcatc caaaatcagg acgttgattt
      241 tttctaaaaa gagtttcagc aacgccaaac ggattttttc accaccggat aaatctttga
      301 tccgtttacc cacatcgctt tgataaaagc cacttttggc taattgccgt cgagcgactt
      361 cttcgtttcc agtcaatgtg atggcgtaag ccagcaaacg gctatcggga tcggcaaact
      421 gcagctgctg cggcaaataa ccgatttgca agctactacc taatttgatg gtgccaccgt
      481 aaggcttttc ctcaccgagt aataatttga gtaaggttga ttttcctgca ccattgtcgc
      541 cgatgatggc taaatgatct ccacgataga ctgcaaaact gctgtctttg aatagtactt
      601 gatcagcgta ctgctggtgc aagttttgaa tgatgaaaac ttctttgcca gattttccac
      661 cattagacaa gctttgcagc cgatttttcg ggggaggtgg cggtttgacg agtgtcagtt
      721 tcgccaggcg gcgttccagt tctttggctt ttttgaagaa ggactcattg tctccttcat
      781 gtgcccactg acgaaaacga cgaatcatga ctttcaaccg ctggacttct ttttgctgca
      841 gttcgtaatt ttttgtcagc tcagccaagc gagcttcttt caagactcga aaacggctgt
      901 agtttccggg atactcaatc aatgcacctt cttccagttc ccatgttttt gtggtgactt
      961 gatcgagaaa ttgccgatca tgagaaacga ccagaaccga tttttttgta ttctgtaaat
     1021 agttttccaa ccagcgaata ctgttaagat ctaaatgatt ggtcggttca tcgagtaaaa
     1081 gtaaatcggc atcggctgct aaaattttgg ctaaagcgac tttaactcgt tcgcctccgc
     1141 taagttctgt taaccgtgct tcctgacggt gtggaatccc taagccttgc aagatcgctg
     1201 caatccggtc ttctaatacg taaccgccta gttcttcaaa gcgatgctgc agctcaccgt
     1261 aacgggccag acacttctct aagtttgtat ctggttctgt catcaatgct tcgtagcgac
     1321 tcatttgctg tttgaggtcc tgtatctcag cggcagaatg gagcaagtag tggttgacgg
     1381 tcgcttcgga aactgtaagt tcttgttcga ccgtcccgat ctttaagcct tttttgcgac
     1441 tgataacacc gctgtcgaca ccttctctgc cggtcaagat ttgtaaaaag gttgatttgc
     1501 cagttccgtt gataccgact aagacaattt tttcaccagc ttcaatttga caggataaat
     1561 cctcaaataa tggattcccg ccgattgttt ttttaataga aattgcttgt atgatcatga
     1621 tctctctcct tgagaccact caaaaaggcc atggttatcc atggcctgtg ttatttaaac
     1681 tcagattatc atgagaaaaa taccttgaga tggccattga ttttacgtga attcaactgt
     1741 tttggacaaa cttgtcccgt caacagcaac tgtaaaacca attgcatggc taaatgcgat
     1801 atggtatcgt tggtaatttt cattacttgt tattttagtc atgcgcatca tagtattttc
     1861 cctccttcgt gtagttgtct tttccagtgt acctgaaagt gtcagaaagt tcaactagcg
     1921 ctgacattta cttaatattt atcgatgtgt aattctgtca gagagctttt tttatctgac
     1981 tgagggtaat gtctcgtgga ccacatcatt gtattctgag ctaaaccagc gttcaggcga
     2041 aatcatatgg tgtttgataa aaaactttgt ctacatgact tccttttgcc atttgcttaa
     2101 taccgttgca ttaaacagct cttctctttg gaaatctcac caacatcgcg ttcaggagta
     2161 tcgttgtggt tgcaatggca agcggtagcc tttcatcaat tcttgaatga tttaccgaat
     2221 gtagcacttg ccttctcgcg gtacccgaac ccgaaataaa attttagttt ggcgataaaa
     2281 tcggtatccg ccatcgatac ttcatatttt ttcgtatcag attcatcgga aacttccaca
     2341 ttgacgaacc aataagcttt ggcatgctta ggtcgtttta aaccacacca gggcgactaa
     2401 tcgccctggt gttcactttt ttttatttta cttgaactca ttgactgttt tccctttatt
     2461 ttaaacatta taaagaccga tgagtgcttg ccgcgtcttt tgtttttatc acggtttttt
     2521 gtgcaaaaaa acctgccgat agttggcagg tcgtctacgt gttaagcttt ggtgtggtct
     2581 ttagctggca attggttttc taaatcagtt cggacaacaa gcacatcgca agctgcgttt
     2641 ctaataacat attcagaaac agaaccaata aataaacgtt ctacagcatt caatcctgtg
     2701 gctcctaaca taatcaaatc tacttgatta tcttctggga tttctctggc aataatttgt
     2761 tttggtgagc catattctac gacagaggtt acattattca agccagcttt cttcgcattg
     2821 ctttcataat ctgccaatgt ttgctttgcc atttcagtag cttgttctgc aagcattcca
     2881 tcaaaggatg aaacggtttg gaaagaacgt gtgtcaatga catgtgctaa tagaagttct
     2941 ccgttatttc ggatagctac attgaccgcc tttttaaaag ctaattcagc ttcatcagaa
     3001 ccatctacgg caaccatgat ttttttgtat tgttgtaaca taagcattca ctcctcaagt
     3061 caatcgtatt gtactctttt attgtagaac actttaggga aaaagagaat tatttactgc
     3121 tttggttcag caattttttt tgcaaatcgg atactgaagg tggcagaaac aataattacg
     3181 atcagtaaaa agaccaaggc aataaagcgt tgattgaaaa aagctgttaa aatacccatt
     3241 acgcctaaaa cagcatatgc agctccgtaa aattgtagga atccttgatt tttatcattt
     3301 ttgttgatca atacaaaaaa gatatcctgt ttattaaata aataatagct aacgaataat
     3361 aatactaaag tggcgataat cattaaaatc ctaatcatga acaaatcctc caaaataagc
     3421 aacaaataga aaaagcaatt tgccaagagc aaattgcttt attctttaaa agatggtaat
     3481 ctccgttaat gccgttgttt gtcctgtagt attgatcccg cgaattcaag caggtgggtc
     3541 ccatggtcgt agcttcgaac taccaaatga taaggcatgt taccagcagt gacacaagac
     3601 gggtcccaag atatcaataa aaatgttcgg tcaacacaca aagaggacaa ttcgaacatc
     3661 acagatttcc cacctctata gtagcatatg aatttaaaaa gagcaatgag agagctcgca
     3721 gaaacagaac cgttttcttg tctagttttt tggaggatgc tttttccact cttttatacg
     3781 gtaatactgt tgacccagag cctgttcata tttccctgta tcttttggct gataatactg
     3841 agcattcttc agtttatctg gtaaatattg ctgattgacc caagcctgat caaaatgatg
     3901 aggatactga taacccacgc cacggttgag tgatttcgca cctttgtaat ggctatctct
     3961 caaatgatca ggaacatctc cagctttgcc ctctcgaata tcagcaagtg ctgcatctaa
     4021 tgccatgtag gcagaattgg attttggcga caagcaaaga tcgacgacga cgtctgctag
     4081 tgggatacgt gcctcaggca atcccaattt ttcagcagct aatacagcgt tcactgtacg
     4141 agccgcagca gcaggattac ctaaacctat atcttcataa ccgataacca tcagacgccg
     4201 gcaaatactt gccagatcac ctgcctcaac taaacgggcg agataatgaa gtgcagcatc
     4261 cacatcactt ccacgaatcg atttttgaaa agccgaaata acgtcataat gagcatctcc
     4321 atttttgtca tgggtaagag cttttctttg gacacattct tcaatgatcg acagtgtaag
     4381 atgaatcctt ccttctttat ctgaaggagt cgatagtgta gccagttcca gcccattcaa
     4441 tgcactccgc aaatcgccgt tcgttgcacg cgaaaggtga agaagagcat cttcgtctaa
     4501 ctggattgct tgttgtccaa ggccgcgttc tttatccctt aaagcatgtt cgactgccaa
     4561 ctggatgtct tgttctgtta atggtttgac ttcaaagatc tgtgtacggc tgcggatcgc
     4621 tggattgatg gtaatataag gattctcagt agtcgctccg atcaagatga ttcggccact
     4681 ttctaaatga ggaagcagaa aatcctgttt tgttttatct aatcggtgta cttcatccaa
     4741 taagaggatc actgtaccac tcatttttgc ttcttccgct acaacttgta gatccttttt
     4801 tgaatctgta gcggcgttta gcatgcgaaa tgcataatta gtagagccgg cgatcgcact
     4861 tgcgatactc gtttttccag taccaggagg tccatataga atcatggaag agagcatgcg
     4921 agcatcgacc atgcggcgta tgatttttcc ttccccgact agatgctgct gaccaacgac
     4981 ttcatcgatc gttcgaggtc tcatgcgata agccaatggc tgttgcatag gattttcctc
     5041 cttcttgttt ttacgtccct taattatatc atatttagca agatgcctag aaagaacagc
     5101 cttctgttgt tttgttttca tagcttatcc aactaatatt gtgtatgata aagtaggtga
     5161 gaaaaatgac ggataaacaa aagatagatg aatttttttg gaataaaacg acgccagaag
     5221 tggcacggga tttattgggg atgtatttgg agcatgacac accagaagga agattagctg
     5281 gatatattgt agacgcggaa gcatacctag gtccagaaga cgaggccgcc catagctatg
     5341 gtttaagaag aacaccgcgt gtgcgggcaa tgtatgaaaa accaggtacg atctaccttt
     5401 acacgatgca tactcatcgg attttgaaca tcattactca gccagaaggg attcctcaag
     5461 gagtaatgat acgtgcaatc gaacccgccg caatgattga tcaaatgagt aaaaatcgtg
     5521 gcggaaaaac tggtccagat atcagcaacg gtccgggaaa attagtggaa gcattagcaa
     5581 ttcctcaaga actttatggt cagtctatag cagatagttc gttacgctta gtttttgaga
     5641 aaaaaaagac accaaaaaag atcaatgctt tgcctagaat cggtatccca aacaaaggtg
     5701 tatggacaga aaagccgttg cgttttgttg tgtcgggaaa tccttatatc tctttacaaa
     5761 gaaaaaacca aatcgagaaa gattggggtt ggaggaaaga aaatgaaaaa gaaggatcta
     5821 ttaacatatt tagatgaaat aattgaaaaa caagtgacag actatgatgt agctttagat
     5881 tgggatacaa aaaatcatac gatcgagatc gttgttcgct tgtttgcaga aaataaagca
     5941 caatttgaga ttgatgatgc agaagggatc gtttcgcaag aaccaattat cgaatttgaa
     6001 gacggcgtac ttctttttaa tcctcagaaa tctgtttttg atgaacgcaa ttatctagca
     6061 gtaatccctt atgaaggaaa aaaagggttg gcaaaatcag tagctgattc tttagtggaa
     6121 tatctaaatg aagtattagc tcaagggcaa agcgatttgc ttgatttttt ggatgaagac
     6181 gatgacgaag ctgtattcga gcttcactgg gataatcagc aactagcaga attagtagaa
     6241 gtaaaacagc aaaatgaagc agatacttat ttaccgtacc caagttatta aggagtgaca
     6301 gatatgaaat ggaatgaagt aaaagtagaa actgaaagtg aagcagtcga agcagttgct
     6361 aatattttga tggaggcagg ggcgagtggt gtcgcgattg aagattcttt agatgttgaa
     6421 cattttcagt cggatcaata tggtgagatt ttagataaaa atgattttac atcgctgaaa
     6481 gaaggcgcac tagtcatggc ttattttccg gaaacgactt tcttaccgga aatcttgcct
     6541 tttatcaaag atcgtatcac acaattacct gagtttggtc tagctattgg aaaaaataaa
     6601 gtaacagtta gtgaagtaga ggaaagcaac tgggcaacag cctggaagaa atactatcat
     6661 ccggtgcgta ttacgcgaat gctgacggtt gcgccaagct gggaaaacta ccagacagaa
     6721 gatcctctag aaaaaattat cacactagat cctggtatgg catttggcac aggcactcat
     6781 cccacgactt ccttgacatt gacagcacta gaaacagtct tgcgtggtgg tgaaacattg
     6841 ctagatgtgg gaactgggtc gggcgtgcta agtattgcag caaaatatct tggtgcaaaa
     6901 gatgtctatg cctttgattt agacgaagta gccgttcgtt cggcgaaaga aaatatggat
     6961 atgaatgaaa tagccaaaga tgtccatgta tcagctaacg atcttttgac aggaatcgag
     7021 attgaaagtg atgtaatcgt tgcgaacatt ttagcagata tcatcttatt gatgatccca
     7081 gatgcttggc gtttactgaa gcaaacaggc acgctgatcg tttcaggaat cattgaagag
     7141 aagaagcaaa tggttctgga tgccatgtta gaacaagggt ttatcgtgga tcaaattttc
     7201 cagcaaaaag actggtatgc aattattttg aaaaaaccag aggtggaata gatgcagcgg
     7261 tattttttaa aagaatcata tgaatcaaaa gactattttg ttttagaagg ggaaaactat
     7321 caccacattg tacgggttat gaggatggaa cccgaggatg aagttttttt ggcattcaat
     7381 gatcaaatag ccattgttgc agaaatcacg gagattactg aagaggctgt ttatctgaaa
     7441 gaaattaaaa aagaagaaaa tcaaaaggaa ctgccaagtt cgattaccat agcgagtggc
     7501 tatcctaaag gggaaaagct ggattggatc gttcagaaat ccactgaatt aggagcgcaa
     7561 gcttttcttg gtttcccggc atcagcctct gtcgttaaat gggatgcaaa aaaacgtatg
     7621 aaacgacaac aacggcttga aaagattgct caagaggcag cggaacaatc ccatcggcag
     7681 atcgtgccat cagtagagtt attggaaacg aaacaagatt tatttgagcg gctagatgat
     7741 tatgacgtgg tcttggttgc ttatgaggaa tcagcaaagc aaggcgaaaa atcaaatgca
     7801 gccaaaattc ttagtgaaac aaaaccagat agtcgaatct tagctgtctt tggaccagaa
     7861 ggtggattat cccgcgatga agtcgattat tttgtagaac acaaagggat actttgtgga
     7921 ttaggaccaa gaatccttcg tacagaaaca gcacctcttt atttattaag tgctgctagc
     7981 tatcaatggg aattaatgaa tccttctttc agctaacgtt cttcattaat aaaagagtat
     8041 cgacagaaag aattgaaaaa atagtcaaca atttgtataa tagaaacaat tatttataca
     8101 atggatacga aaaatcgttt gaaaaagctt ttaaaaagcg aacatttccg taaaaaaacg
     8161 tataactgca cggattttgg gacaattatc aaacagatat ttgtcccgat tcccgtttta
     8221 ccgaaaaaag aaagggggat cgattcctgt gacaaaagaa gaaattttaa cgggacccgg
     8281 tgtcataaag ctcgttagtc aatacatggg tcccgaacat gtagcatttg ttgagaaagc
     8341 atgcgaatac gcgactgctg cacatgacgg acagtttaga aaatcaggcg aaccctacat
     8401 tattcatcct atccaagtcg caggtatatt agcagattta aaaatggatc cccatacagt
     8461 ggctacaggc ttcttacatg atgttgttga agatacagaa atcactttag aagatctgag
     8521 agaagaattt ggcgatgacg ttgctatgtt agtggacggc gtaaccaaat tagggaaaat
     8581 aaaatataaa tcccacgaag agcagctagc agaaaaccac cgaaagatgc tgctagcaat
     8641 ggctcaagat ttacgagtta tcatggtcaa attagctgat agactacata acatgcgtac
     8701 gttgaagcac ctgcgagaag ataagcaaag aaggatcgct caggaaactt tagaaatcta
     8761 tgcgcctctt gcacatcgtc tagggatcag ccggatcaaa tgggaattag aagatacagc
     8821 acttcgttat ctaaatccaa aacagtatta ccgtatcgtc cacttgatgc agacgaagag
     8881 agaagaacgt gaaaaatacg taagtggtac tgttgaagat attcgaatag cgacagaaga
     8941 gctggggatt tttgcagaaa tctatggacg gccaaaacac atttattcaa tctatcgtaa
     9001 aatgaaagat cagaaaaagc agttcaacga aatttatgac ttgctagcta ttcgagtgat
     9061 cgtagattca atcaaggatt gttatgctgt attaggagca atccacacaa aatggaaacc
     9121 aatgcccggc agatttaagg attatattgc catgccaaaa gctaatatgt accaatcttt
     9181 gcatactacc gtgatcggtc cggcaggaaa tccggtagaa attcaaatcc gaacacagga
     9241 aatgcatgaa atcgctgaat tcggggttgc tgcacactgg gcctataaag aaggaaaaaa
     9301 tgaaaaagta gaaccagatg gtatgacgaa acaattaagc tggttccatg agatactcga
     9361 acttcaagac gaaagctatg atgcttctga atttatggaa ggcgtaaaag gagatatctt
     9421 tagtgataaa gtctacgtct tcacaccaaa aggagacgtt actgagttac caaaaggatc
     9481 cggaccattg gactttgcat acagtatcca tacagatatc ggtaacaaaa ccactggtgc
     9541 aaaagtaaat ggcaaaatgg tgcagcttga ttacaaattg aaaaacggag atatcattga
     9601 gatcatgact tctccaaatt catttggccc aagtcgcgac tggttgaaat tagttgctac
     9661 tagcaaagca agaaataaga tcaaacgttt cttcaaagcc caagatcgag aagaaaatgt
     9721 gatcaaaggc cacgaatccg tggtcaaatg tattacagat ctaggattta cgcctaaaga
     9781 tattttgacg aagaacaaac tgcaagaagc actcgatcgt tttaattatc aaacagaaga
     9841 tgatctctat gcagctgtag ggtatggaga agttagcccc ttgacgatgg ccaatcgtct
     9901 gactgaaaaa gaacgtaaag aacaaaaaat cgagcagcaa aagcaagaag cagaagaaat
     9961 catgaatcag ccgaaaaaag aacctgacaa aatgaaagta cgtcatgaag gtggcgttgt
    10021 cattcaaggg gtagaaaact tactaattcg tatcagtcgc tgctgtaatc cgattcctgg
    10081 tgatgatatc gttggttata tcactaaagg cagagggata tccattcatc gtcgagattg
    10141 tccgaatgtt cagcctgaca aaccaaatgt agcagaacgt ttgattgaag tcgaatggga
    10201 agatacatcg aatacacgaa aagagtatga tgcagatttg gaaatttacg gctataatcg
    10261 ttcaggctta ttgaatgatg tacttcaaac agtcaatgcg ctaacgaaaa atctcaacag
    10321 cgttgaagca cggacgaata aagataaaat ggcgacgatc catttgacgg ttggtatcca
    10381 gaatttatcc cacctaaaga gtatcgtgga taaaatcaaa gcagtacctg atgtctacag
    10441 tgtacgccgg acgaatggat aggaggacag atatgagagc agttgttcaa cgggtaagta
    10501 aagctgctgt gtcagtcgat cagcaagaaa ttggaaaaat tggctacggc ctacttgttt
    10561 tattaggcat tcatgaaacc gatacgcaaa aagatgtgga ctatctggta aaaaaaattg
    10621 cacaaatgcg tatttttgaa gatgaacaag gaaaaatgaa tattagtgta gaagatgtgg
    10681 gaggacaaat tttaagtatc tctcaattca cactgtttgc ggatacgaaa aaaggaaatc
    10741 gaccaagctt tgtggaagcc gcacgaccag aaacagctat tcctttatac gaatcattca
    10801 atgaaggaat cagaagtcgc ggcatcacag tcgctaccgg acaattcgga gcagatatgt
    10861 cggtttctct cgtcaatgat ggacctgtaa cgatcattat tgattctaaa aacaaataaa
    10921 gtaatctggt tttcactagt aaaaactatg aaacacgtcg cttaagttag actgataaac
    10981 tctaacttaa gtggcttttt tgtctgcaaa aagcgcttct tttgtcagca gtcggaaaaa
    11041 aagttaagct taaattatac aagttatttt ttataggagg ttgctgaaga tgaaagaagt
    11101 accagtagta gatatcaaaa tcacagattt aaaaaaattg tatcaaaaag gatacaacat
    11161 gttggaggag ttacgccatg aagcggacgc tcccgtcgtc aaagcaaaga ttttcaataa
    11221 agaagctatc acgatctacg gatcatcagc agcaaaagta ttttatgatc caaggaattt
    11281 caaacgaaaa ggtgctatgc caaaattagt attgaagaca ctttttggtc aaggtggagt
    11341 acagactttg gatggagctg ctcatcacca tcggaaaaat atttttatgg atctaatgac
    11401 accggaaaga atggaagatt atcatcgtat cttagataag aatctgacac aagcacttga
    11461 agcacagcat ggtcaatttg aactatttga tttaagtaag atggttttct ttacatctat
    11521 ttgcgagtgg gcaggtatca atttatcagc gatttctaaa gatgaggtcg aaaaattagc
    11581 agaatatcaa atctctatga tcagcggaac gtttacttct ccgattgatc acataaaagg
    11641 tgtcgaaaat cgcaaaaaat cagaaaaatg ggcgcaaggc ttgattgaag aagctcgaca
    11701 aaatcctgtg gctggaaaag aaaatgtggc attatatgct tttgccaatg ccacagattt
    11761 agatggtcag cttttacctc tggaagtggc agctgttgaa ttattgaaca tcatccgtcc
    11821 aacggttgct ctaactgtgt gggcggcatt gatgggacat gcacttttca gtagaccaga
    11881 tttgtatcag caattaaaaa atgacttttc gacgttacaa gatccgttta ttcaagaaat
    11941 gagacgctat tatcctttct tcccaatgct acctgcgatt tcgctgaaag aagtagaagt
    12001 agatggttat cgtataccag aaggcagttg ggttatttta gatttatatg gaacagacca
    12061 tgatgaacga acagttgaag cgccagattc ctttatgatc aaacggtatg tagggaaagc
    12121 aaaagatatt tcctataaag aagaatatga gatgattgca caaggcgggg gaaattttcg
    12181 acaaatgcat cgctgtgcag gagaatggat cactcttcat agtctacgtg tgttttccga
    12241 tcagctagta aataagtttg agttctctgt acctgaacaa gattggacga ttccattcaa
    12301 tcagtttccg acttatccga atagccgagc gctactttac aagaattaga ttagcataaa
    12361 cttatcgaaa aaaaggaaga tcaaacagtg ctgatcttcc ttttttcata ttactataaa
    12421 ttgataaggt caattgatca aaatccacga aatgcttcat caatggtttg ataatcttca
    12481 ttggataaat gcaaatctaa agcttggaca ttgtcagata cttgctctgg acgcttagct
    12541 ccaggtataa ctacagacac tcgtggattc ttcatgtacc aagctaaaac aagttgcgca
    12601 ggagtggctt ggtatctttt agcaatgggg cgaagtacat cgacagcggt taaaatttct
    12661 ctgaaacgtt ctccttggaa atctggattt ctttttcgag gatctccttc accaaattgt
    12721 ttttcctcac caagttcata ttttcctgtc agcaaaccgg aagcaagagg aaaataggga
    12781 acaaaactga ttttgttttt ttcaagataa gggaataatt ctttttcagc ctgccgatgg
    12841 atcaaactgt atttatcttc tactacatcg acatatccat ctgcattcgc ttctttcaat
    12901 tgttctaacg tgaagtttga cacaccgact gctcggattt ttcctgcttc ttttaattcg
    12961 tgcaatgtag caactgattc attttttggt gtgctttcat ccggaaagtg gatataaaaa
    13021 atgtcgatgt aatcagtttg cagacgtttt agtgcatctt ccaccgattg tttgagaaac
    13081 tcaggagaat tatcaaatgt tcgcccttta tttggtacat gagcagcttt tgtagcgatg
    13141 acgacacgtg agcgatcata ttctttttcc ttcagtacct cgccaatcag ttcttcggaa
    13201 cggccgtttc cgtaagcata agcggtatcg atcaaattga tcccgctatt caaagctgta
    13261 cgaactactt gttttcctgt ctcatcttcc aaaccgtcaa aaagattatg tcctccaacg
    13321 gcatttgctc ctaatccaag ctgttctgcg tagacttgtg aatgtccgat tcttatttct
    13381 gccatagctg attcctcctt tttgtctagt ctaaaacaaa ataccaaaaa gctgaactaa
    13441 aacgtttttg tgaggggaaa tagtcagata tctcttgact ttgagactgg tttttagtag
    13501 gttataagta atatcgaatg tcaatgaaag agcgagtaag tttttgaaac tttacaagag
    13561 aggatcatcg aggctgaaaa tgatctaaag tttcattagc ctaaagacac tctggagatg
    13621 gacgagtgaa aagccgtccc gcgtacgagc gttattcgta agaggaaaga gaaatctttc
    13681 aaaattaggt ggtaccgtgt atgaatttca ttcacccttg ttcatgatga acaagggtga
    13741 tttttttatt tacatagcta agaaagggag agaaaactat gagtttccaa agaccaaaag
    13801 gaacaaatga tattttgcca ggcgaatcag aaaaatggca gtttgtcgaa gaaacagcac
    13861 gtttattatt cagagattat cagtataatg aaatgcgtac accgattttt gaacactatg
    13921 aagtgatatc ccgaagtgta ggggatacaa cggatatcgt ttcaaaagag atgtatgatt
    13981 tttatgacaa aggggaacgc catgtgacgc ttcgaccaga aggtacagca ccaatcgttc
    14041 gttcttttgt tgagcataaa ttgttcggcc cagagtatgc gaaaccgttc aaaacttttt
    14101 atatgggacc aatgtttcgc tacgaacgcc ctcaaaaagg acggttacgt caattccatc
    14161 aaatcggagt agaagcattt ggatcagaaa accctgcaac tgatgtggag agcatggtga
    14221 tggcacttga tttcttcaaa cagttgggga tcacgcaaat ccggctagtc atcaattcat
    14281 taggtgataa agaaacgcgt caagcctatc gtcaagcatt gatcgactat cttttacctt
    14341 ttgaatcaga gctgagtgaa gactctaaac gccgccttca tgaaaatcca ttacgcgtat
    14401 tggacagtaa agacaaacga gatcaaaaat ttgtggcaga agctccatct atcttagatt
    14461 atctaagcga gcctgctcaa aaacattttg ataccgttgt ttccatgctg gatgcattgg
    14521 ctgttcctta tgaaatcgac agtaatatgg ttcgcggact agattattac acacatacga
    14581 tttttgaaat catgagtgac gcaccaaaaa tgggggcaca agcaacgatt tgcgcaggcg
    14641 gacgttatga caatctagtg gaagaactag gtggtccttc aacacctggc ttcggatttg
    14701 caatgggtat cgagcgatta ctcttgacta tggaggcaga ggaagtagtt atccctgcat
    14761 ttaatgagtt ggatgcttac gtggtcgcac tcggagacga aacaaatatc gaagcattga
    14821 aagtggtcca agcgattcga aactttggtt tttcagctga ccgcgacttc atgaaccgta
    14881 aagcaaaagc gcaatttaag acagcagata aagcgaatgc caaattagta ttggtgatcg
    14941 gtgaagatga gcttgctagt caaacgatta atgtcaaagc aatggcaagc cgaaaagaaa
    15001 aaagttttcc attagaagag atatatgaac attttgatga agtctatgat gaaatgactt
    15061 tattcgagta ggaggaaaaa aataaatggc acaaagaacg atctattgcg gattagtttc
    15121 cgaacaacaa ttagaacaaa cagttacatt aaaaggatgg gtacaaaaaa gacgagacct
    15181 tgggggagta atctttatcg acctacgtga tcgtgaagga atcgttcaaa tcgtctttaa
    15241 tcctgaaatc aacaaagaag catgggaaat cgcggataaa tgtcgcagtg aatacgtgat
    15301 cgaagtgact ggggaagtta gaagacgcgg agaaaatgca gtaaatccta aaatggccac
    15361 tggtgaattc gaagtgattg caagtgaaat cgtgattttg aatcaagcaa aaaccccgcc
    15421 atttcaaatt gaagatgatc aagcaatcag tgacgaaata cgtttgaaat atcgttattt
    15481 ggatctgcgt cgtccaaaaa tgactaaaaa catcaaatta agaaatgcga caacaaaagc
    15541 gattcgtcat tatttagatg ataatgactt tctcgatatc gaaacacctt acctagggaa
    15601 atctacacca gaaggcgcaa gagactacct agttccttca cgtgtccatg ctggacattt
    15661 ctatgcgttg ccgcaatctc ctcaattgtt taaacaattg ctgatgaatg ctggattcga
    15721 ccgttattat caaatcgttc gttgtttccg cgatgaagat ttacgtggag accgtcagcc
    15781 tgaatttacg caagtagata ttgaaacaac gttcttaaca gcagaagaaa tccaaactta
    15841 cacagaggga ttgattgcga aggtaatgaa agaagttcgc ggtatcgaag tcacattgcc
    15901 atttcctcgt atgacctatg atgaagcaat ggctcgctac ggcagcgaca aaccggatac
    15961 acgttttgat atggaattga tcgatctaag cgatacagtc aaagaagtag aattcaaagt
    16021 cttccaaatg gctcttgaaa acggcggtgt cgtgaaagca ttgaatgcta aaggcgctgc
    16081 agatcgctac tcacgaaaag acatggatca gttaggtcaa tatgtaggac aattcggagc
    16141 taaggggctt gcatggctga aagtagaaga agacggattg aaaggtccaa ttgctaaatt
    16201 catgggagaa gcaacagaag caatcattaa agcaacagat gcaaaaccag gtgacttgct
    16261 gatgtttggc gcagataaaa gtgaaatcgt cgctgctgca ttaggtgcta tccggacacg
    16321 tttaggaaaa gaattaggac taattgatga atcgaaattc aatttcttat gggtaactga
    16381 ttggccacaa tttgaatttt cagaagaaga aggtcgatat gtctctgctc accatccatt
    16441 cacgatgcca aaagaagaag atatcccgtt attggctgaa gaccctgcaa aagtctatgc
    16501 agaagcttat gatatcgtct tgaacggtta tgaactaggc ggaggttcta tccgtattca
    16561 tactcgtgag cttcaagaaa aagtactaga aactctaggc tttacaaaag aacaaatgga
    16621 agaacaattt ggtttcttgt taacagcttt agattacgga ttccctccac atggcggtat
    16681 tgcattagga ttggaccgtt ttgcaatgtt attagccgga gaagataata ttcgtgaagt
    16741 cattgccttt cctaaaaacg gaaaagcagc agatgtgatg agtgatgcac caagtatggt
    16801 ttcgccttta caattgttcg aattgaatat tgatgtaacg aatatagaag aatagccgat
    16861 aaaaaggtaa aaaggatttg tcgaatacgc ttcttttgac aaccttgatt taattgaaaa
    16921 acactttata atgaagccat aacatataaa gcggggcttt tccatgaaaa aaacattcga
    16981 aaattgggct tatcatgatt acacgaccaa gctttcggta tctatcgttt atgcgttgat
    17041 cgcatcagtc gcattgaatt tcttttatca gccaggacat atttattcaa gtggtattac
    17101 tggtctagct cagattttga cgactttgtc aaaatcactg ataggattcg aagtaccggt
    17161 atcaacaact ttgtatttac tgaatatacc tttatttgta ttggcgtggt taaaaatcgg
    17221 gaagaagttt acaattttca cttttatcac agtcactttg acctcccttt gtatccatat
    17281 tttaccggaa acagtcctat ctaccgatcc aatcatctgt gcgttgtttg ggggggctgt
    17341 gatgggacta ggtattgggc tagcgttgag aaacggccta tcatcgggcg gtctagatat
    17401 cgtgagtatc acgattcgta aaaaaacagg aaagtcgatt ggttctattt cgatctactt
    17461 caatgcattg atcgtttttg ttgccggaat tctatttggc tggcaatata tgttttacag
    17521 tacgttgtct attttcgtca gtggaaaagt aactgatgtc gtttatacta aacaaaagaa
    17581 gatgcaggta atgatcgtaa cgaaacatcc agaaaaagta gtaaatgaaa tccaaaactg
    17641 tatgcgtaga gggatcacga tcatcaatga agcagaaggt gcttataagc atgataagca
    17701 gacagtattg atgacgatcg ttacacgata cgaactgcct ctacttagag aagcaatgaa
    17761 agcttctgat ccacaagctt ttgtaagtat tgcagaaaac gtccagatac ttggtcgttt
    17821 ctatgaggat ggcatttaga tcgagtttga caaaaattca tgaaatactg ctgtagacag
    17881 acgttcgttt gtctgcagtt tttttctacc atagtagaat cagcaaagaa aaactgcttt
    17941 tttatttgaa attttcttaa agttgaaaaa aagcatgttt atttttttaa atttatggga
    18001 tgatagaaaa aaatagtaga gaaaggacac gattctactt gctattttca cttaataccc
    18061 gtatattacg aatgaaaaaa gtttatgaaa agttggaata ttgaatggaa aaacaaaaaa
    18121 ggttaaaaaa cagccgctac atcactgggt ttgatgggct ccgttcatta gctgtggtgg
    18181 gtgttatctt ctatcacctt ctgccaacta gtatgaaagg cggctatttg ggcgttccga
    18241 tttttttcgt cgtatctggt tatttgatca cggatctgct gcggcaggaa tgggaacaaa
    18301 atggaaaaat caatatttgg caattttatg tcagaagaat gaagagactc tatccggcac
    18361 ttgtttttat gcttgtgaca gcttcggctt atatcacttt gtttcagcga gggctgttaa
    18421 acaatcttcg aggagttgtg gtcagcagct tactgtatgt caacaactgg tggcaaatca
    18481 ataacggttt atcttatttc gatcgatttg ctaatgagtc accgtttacg catatctggt
    18541 ctttagcagt agaaggtcaa aactatttgg tttggccgat cttgtttgta ttgttgatgg
    18601 tatttgttcg taaaagaaaa tggatctttt atactgtgtt aggtacatcc ttcctctccg
    18661 ccatcttgat gatgatcttg tatacaccag gaggcgatcc aacccgtgtt tattacggta
    18721 ccgacactcg tctgttttct atatggttgg gaagtgcgct tgcgtttgtt tggccaagta
    18781 cgcgtttaaa gaaaaacatc ccgaaacaag cgaaaaatat tctgaatatc gctggaggaa
    18841 tctcgctttt ggcactgatt ctttgcttct tctttttaga tgatcattac aatttcatct
    18901 attacggagg aatgctccta gtgagtctgt tatgcgtagt gctagttgca gtgacagcac
    18961 accccggagc aagtttgaat cgatggctga caaatccagt atttacttgg attggcaaac
    19021 gaagctatgg catttatctg tatcagtttc cagttatgat tttttatgaa gcaaaggtga
    19081 ctgatattgc cgatcatgtt ttgttgcata ctttagtgga aatcacattg attctaatca
    19141 tcagtgaact ttcttatcga tatgtggagc gtccgctagc acgttttgat tatggtcgta
    19201 cattccaagt gatgaaagga tggttcagca aaccgatcat cagtaagagc aaaccatggg
    19261 ttgttccagg cacattactt gtagcaattg cactcgttgg tttcgttacg gcaccgaaaa
    19321 acgctgtaac agccgatcaa aaacaactgc aggcaaaaat cgctgaaagt aaaaaattag
    19381 cagaagaaac cagaaacaat tcagaaaaca ccactagttc ggttgaccaa gcgatccttg
    19441 ataagtacga gttgtccaag gcacaaggga ataaggcaca aaaattagaa ctaacggcat
    19501 ttggtgattc agttatgttg gatgctgcaa cggatctaca agaactttat ccaaaagtag
    19561 tggtagatgg agatgtcgga cgacaattat atgccagtgt accgtacatt gaaaaattaa
    19621 aggaacaaga tttgttgaaa gacactgttc tgatcggact aggaacgaat ggctcattct
    19681 cggaagctca gtttgatagt gtgatggatg caatcggtga tcgaaaagta tactgggtca
    19741 atgtccgagt accgacgaag cgctggcaaa atgacgtgaa tgcgatgctt gaacagatga
    19801 cgaaaaaata cgacaatatg acgctgattg attggtacag ctacagtaat gaccatagtg
    19861 actggtttta tgatgatcaa gtccatccaa atcctgaagg gatgacacat tacattcact
    19921 tagtcagcga agccttgcta ggaggaagtt caagtacgaa cagcagtgag acaaacgtga
    19981 cagaatgata gctacaaaaa acttcaggca aaaacatttg tttttgcctg aagttttttt
    20041 atatttaccg tgtttttaac attcatatag attcaggaag caaaacggtt gagtgtgtct
    20101 gtaaataacg ttctttttgt tttcgggtga gttttttgaa tgcagcttct gcttttgttg
    20161 cttcgcttct tgaagaaaat acttctgcat ggatcatctt aacaggtctt cttttttgaa
    20221 gacgagtata tttagcacca gtaccattgt tatgttcctc taatcgtcta gaaagatcgg
    20281 ttgtatatcc accataaaat gtctgatctt tacagaataa tacgtaaaaa taatgttcat
    20341 ttgccataga gcatctcacg aatctctgca gtgtattctc catccatatt gtaagtgatc
    20401 aatggtggag cgactttgaa tccgtctaat ttgccttctt tgattccttc aactaacaaa
    20461 atatttgcct cttttccagg ttttgggtaa acaaattgga tccttttagg tgcaattcga
    20521 tagtcttcca tgacctgaat gatttctaag aaccgatcag gacgatggac catagtaaag
    20581 cgtccatttg tttttagtag cttacttgaa atatcgatca cttcttctaa cgtcgtatga
    20641 atctcatgac gggcgattgc caggtgttta ttagggttcg ttttatttgt tggcagccct
    20701 ttgaaataag gtggattaca gacgactaag tcacaagaat tatgtcgaac gacagataag
    20761 ctgtctttca gatcgatggt gtggacagtc atttgttcat ttaaatggtt aagcgcgata
    20821 ctacgtcttg ccatgtctgc tagacgaggt tgaagttcga tagcgtcgat agccgcctgg
    20881 gttcgtttac ttaaaaaaag tccaactgcg ccatttcctg cacaaaggtc aacaatcttc
    20941 ccttttttag gtacacggga aaagtgagca agcagcacag catctaaaga aaaagaaaac
    21001 actttggaac tttggataat cttgatatct tctgcgtata attggtcgat tcgttcgtct
    21061 tcgtgaagca tggattccct ccaaaatgaa aaaaatagtc gtctgtatta tactattttt
    21121 attaaaaaga accaaggtgg cttgtcaaac ctctattttt tatgcatact atgaccataa
    21181 gaagggaaag gaagagacta tgttttatcg atttatgcgc ggggtagttc gagtactatt
    21241 agccttagtt aatggaaatg cccgctatga gaataaagaa gttttaccgc aagatgagaa
    21301 ttacatacta gttgctcccc atcgtacttg gtgggaccca ttatatctgg cggtagctgc
    21361 agcacccaag gaattcagct ttatggcaaa agaagaactg ttcaaaaatc cagttctgcg
    21421 atttatatta actcgatcca atgcttttcc agttaaaaga gaaaatccag gaccaagcgt
    21481 cattaaaaca cctgtaaaga ttttgaaatc gacaaatttg agtttgatca tgtttcccag
    21541 tgggacaaga catgctacag aacttaaagg cggaatggca ttgatttcca gaatgtctaa
    21601 agcgaaaatc gtgccagctg tatatcaagg tccgttgaca cttggggatc tgttcaagcg
    21661 tcagagagtg acagtgcgtt tcggcgaacc aatcgactta tcagatatca agaaaacgga
    21721 taaagaaggt atggcggaga tcgaacgacg aacacaagca gcttttgaca aattggacaa
    21781 agaagtgaac cctgatttca aatatgaagt caaaaaaaaa taaaatgata agtttatgcg
    21841 tgggcggtct tttgaatcta ctgtcctgtt cagttcagac tttttcaatg aaaacgaggg
    21901 agagacgtca agtctgccct ctttttgcgt tccaatatgt aaaatagaag acaggtgtca
    21961 tgtaatggat caaagtgtta taataattag aaggacattt tttgaggtga ttgaatgcgt
    22021 tttttacata cagccgattg gcatatcgga aaaaaactac aaggttatga cttgattgaa
    22081 gaccagcgtc atgtactatc ttctatacta gatatcgcga aagaagaaaa agtagaggca
    22141 atcgtgattg ccggagactt atatgatagg agtgtcccag ctgttgatgc cgtagaatta
    22201 tacaatgaat tgatggtcga ttggaattta aaggaaaaga tcccgatttt tgctatatca
    22261 ggaaatcacg atagcagtac gagactttca gcgggtacgc catggttcag ttattcgaac
    22321 tattttttgc gaacacagct agcagaagca tttgaaccag tcgagtttaa aaacgtacaa
    22381 tttttctttc ttccttactt tgaaccaatt gcagcgagat tatattttga agatgacaat
    22441 atccgaacga tcaagcaagc ggtagccaag gtgatagata aaatgcagga gctatttgat
    22501 tcagaaaaaa aacaagtatt gattagccat ttttttgtaa tgggaagtgt aaaatctgat
    22561 tcggaaacga aaatcgaagt aggcgggttg gattcgatcc ctggtgaact acttacaccg
    22621 tttgattatg tagcactagg acatttgcat aatcaagctg ctttgagaca ggataatgct
    22681 cgctatagcg gttccccatt gaaattctct ttatctgaga tcaaccaaaa aaaaggcgta
    22741 tggatcgttg acctgacaac aggcctggag ttgaatttta gggagatcga acccctgcat
    22801 gatattgaag aaataacagc gagtttcaaa gaattgctgg atcctgcctt ttatcagtcc
    22861 gtcaatcggg agaattacat ctatatctat ctcactgata gaacgatcat cccgaatatg
    22921 atgaaccagc ttcggcaaat atatcctcgt attttgggga ttgaacgggc aaacggtcga
    22981 gcagaacaaa caaagcgcaa acgaaaagta ctagaaatga aagatcctca aaaattagca
    23041 gaagcatttt ttagtgaaat gacggaagag cagctaactg caaaccaaga aaaatggctc
    23101 ggagaaacgt tgcaagaaat catggaaatg gaatgatcaa gaatgaaacc gaaaaaatta
    23161 caacttaaaa attttggtcc ttttatcaat gagaccgtag atttttcacg tttgacagaa
    23221 gcaccgcttt ttttgatcag cgggaagaca ggagctggca aaacgacgat ttttgatggg
    23281 atcaccttcg cactttttgg tgaaacttcc ggaagacttc gctctggaaa agagatgcgt
    23341 tctttgtttg ctacaccaga cgaagaaaca agcgtgactt tttcttttga acatcagcag
    23401 atgagctatg aaatcgaacg aaaaccagaa caagttttag caaaacgaaa aggaaacgga
    23461 acaaaaaaac aggcagccaa agtaacgttg accatttttg atgggaaagg aaaagaaata
    23521 aaacaaatca caaaacgtac agaagcagat cagttgatca gagaactgat gaatctcgat
    23581 gcgaaacagt tttctcaaat cgttttacta ccccaaggag aattccgtaa ttttttggtt
    23641 tcatcaagca gtgataaaga gactgtttta cgtcatttat tcggtactca atttttccaa
    23701 cagttcaatg agcgattaaa agaaaaagca aaacaacagc aacaaaaatt ggatcattta
    23761 gagcaagagc tgcatcttct gcaaaaaagg tttgttttag tcaaagaaga ggaaccagca
    23821 gcagggtttg aagccgtttt agcccaatgg acaaaccatc aggcaatact cgaaaaacaa
    23881 atcacggaag aaaaagaagc tctaacggaa cttcaggaaa aacaaaagca actagaaaat
    23941 tcctactatc gttttgaaag cttgaaaaaa agttatgaag aaaaaacgat attaataaac
    24001 aagcaaaagg aattagctgc tcaaaaagac gaaatagagg aaaagaaacg atggattggg
    24061 tattatgaat ttacgcaacg attagtcgaa ccaatccagc gcctacaaga aataaaagtt
    24121 gaaaaaatag aactgtcaca aaaaagtagt gagcaaacaa agaaactagc gatgactacg
    24181 cagaattatg aagagtggca aaaaaatgaa gcaaaccgcg tacgccgaca aacaaaaatc
    24241 aaagaagctg ctcaaaaact gcaaaacaaa cagatgatgg tgccaattgt cgaatcctta
    24301 cgtagtaaaa aacaagacgc agaacaatta acaatttcat tggataaaga agaattgaat
    24361 gccgagaagc tgtatcaaaa tctcgctatc tatcaggaaa gaactcgaat tcttcaagaa
    24421 aatttactgg atcaggaaaa actgcaagaa gatcgtcttg cttttggaca gttaaaatat
    24481 ctagatgaaa agcagcaaga aatagaaaaa caaaaagcaa aagacactgg gtcgctcgca
    24541 cagataagag aagaactcaa aaaagcaact gaagaaataa caaaaaatag tcagctattg
    24601 aaagaacaga cagaacaagc agaacagctg aaaagccaat gggcaaagca gcaaattgct
    24661 cgtcttcagc tttttttgcg tccaggggag ccatgtcttg tttgtggatc aacggaacac
    24721 cccatacaac aattaaaaca cgaacagcca aataaggaag atatcttaaa agtagaacga
    24781 caactggcag aagctgagtc tgctgtagaa agaactcagc aaatcattgc caaaagtgaa
    24841 gcacatgaaa ataatcttca acaacaatta tcagatttag aacaaaaaat catggcaacg
    24901 aatatacaat tggctgaaca agaagaacac ttgcaacagg ctttgaaaaa ggcatttcct
    24961 gagataacag aacaaaaaac aaaaacaact cttgtagtaa tcgaacaatc tttagataga
    25021 cgaaaaagca agatggccaa tgcaagaata gagctggaag aaataagaca acaagcagaa
    25081 aagctcgaac aacaattagc tgtctacaaa cagcagcaga cagaaaaaaa taatcagctc
    25141 cagcaactgc aaggagaaat caaaagttta gaacaacaat tgcaagatac ggatagtaac
    25201 tgtttagaag aggagatacg acgacttgaa aaacagttag aagaagactc gaatcaacta
    25261 gctgttgagc agaaagcagg agtgcaattg ttgcaagatc ttcatacgct tcggacacag
    25321 cttgatcttc aacatgaaca aatagaaaaa atagatgaaa aaatagaaaa ggaacaagaa
    25381 agaatagcta aaaagttaga aagccagtcg tatttttcaa caattgaaga aatctgtgca
    25441 tttgcacaac agcaagcaca atataaagaa aaccaacgta cagttacttc ttatcatgaa
    25501 gagcaattag tgacagctga ccggctgcgt caattagaaa atatccagct aactgagaca
    25561 ttcccagata cagaagagat cagccaacag ttagaagaaa taagacgtat gatcgaaata
    25621 aagcaagaaa agatatacgg tttacaagaa caaaaaatta gtaatcaaaa aatctacgat
    25681 gaatgtcttt cgatttatca cacaagccag acgaaattag atgagcttac gcaattacag
    25741 cagctttcgc aaacattcaa cggagaaaat cctaagaaga ctagtttgga acggtatgtg
    25801 cttcaggttt atcttcaaga agtgttacaa gtagcaaatg atcatctcca acgtttaacg
    25861 aaaaatcgtt atcaatttga gctagcggat accattggca gttatcgagg gaaaaccgga
    25921 ttagaaatca atatctatga tgatcaggca ggtatgtctc gaagtgcgca taccctgtct
    25981 ggcggtgaga gttttattgc agcactttct ttagcattag ctcttgccga tgtgatccag
    26041 acacaagctg gtggtgtagc aatcgatgca ttgtttattg atgaaggatt tgggtcatta
    26101 gatgaagaag ctttggaaat ggcaatggaa gctttagaaa cgatcgaaag tgaaggaaga
    26161 atgatcggca tcattagtca tgtaagagaa ttgaaagaac gagtattaca acaaataaga
    26221 attgatacag aaggtagcgg acaaagcaaa atacgctatc aacttggata aaaagggaga
    26281 ttggtaacta tgaaatggag tattccagaa aaagtcattg cacgtggacg tcaatacttg
    26341 caagaaggcc gagtgttatc ggttgtccct gaccaagaag aaaacgtgtg gcatgcagaa
    26401 gtcttgggca gtgaattgta tatggttgat ttggatggta cagcaaaaga gatagattac
    26461 tgccaatgtc cctattggga ggaacataaa tattgtaagc atacagttgc tgtggaactt
    26521 tatttacggt cgaaaaatct atctcgtatc atgaaaaaag accagccaat gctagaaccg
    26581 gtaaaagcaa caagtgaggg agaaatcatt gcaaaaggtt tttctcgtct aaaaacacaa
    26641 aacaatcctc aagcggtgca acctttgatt attgaatacc aagtagaaac gattgagact
    26701 aatcagtatc acccagagct ttctattttg gcactttact tgcgtatcgg ttcattggca
    26761 tcaccgaaaa aaacatatat cgtaaaaaac atctatgatt ttttacaagc ttacacagaa
    26821 aaagaaactt acactgttaa taaacagtac cagtttcgtc tagataaacg ggcgtttcaa
    26881 aaagaagatc aaaaaatact cacgcatctt gcaggaagtg ctcagacgca tcaattatta
    26941 ggtacgaacg gcttacaagt caaaggaaaa ctagataaaa gatatctgct tctcccagta
    27001 gaacaagccc gcttcctgtt agagcagatg aatcagacta cacgtttgca tctgcagata
    27061 aatgaacaaa acgatcaagg aatacaattt tccgatcaaa acaaaccttt atcattcaaa
    27121 gtggaaaaat ttggagaaga ttatttgctg caagcattga atgattttga ttttttcttt
    27181 atgcattatc agtggggagt tatccaaggg acattttatt ctctgacgaa atatcaacaa
    27241 acggtttatc aaacttggaa acaattgatt cgccgattgg agaaaccaga agtagtgttc
    27301 aagaaaaaag aattaccgtc tttgtttaaa gagattatcc ctctgctgtc agagatcggc
    27361 gacgtagcta ttgcccctgc agtagaagag gaagtagcag atatccccgt cgaatttatt
    27421 ttctttttcc gaaaagcaaa aggaaaaatc caagctcgag tcgatttcgt ctatggcgaa
    27481 gtcatctatt cgactgaccc gaagcatgag gtctcgacag aacaggcttt tcaggtgtta
    27541 cgtgacacag tagaagagaa acgagtactt gatctattca gaatgtatcg ctaccagaaa
    27601 aatgaaactg gttatgaacg tccattgcca agcggggaag aactatatgc ctttttccgc
    27661 acagaactat ctgtcttccg tcagtttggt gaagtcagac ttggaagaaa attgcgagag
    27721 ttgtatttag atgcgcataa acatcaaccg aaaatcgaag taaccgagac cggttcatgg
    27781 ctcgatattc gattcgatgt gacagggata gaggagcaag aaatcgatca tgtcctgcaa
    27841 agcttgctga gaaatgatgc tttttataca ttagaaaatg gtcaggttct ttcttttgat
    27901 tcagaagaat ttcaacaaac aagccaagtg ttgcaacaat tacgggaatc gatacgtaca
    27961 gaagaaggaa cgatccatgt acctaaaaat caaggattga tcattcaaaa tcaattggaa
    28021 aaaagcaatg ctacttttag tgaatcgttc caaacgatgg tacaagatct cattcatcca
    28081 gaaagatacc aggcacagct tcccaaagga ttgaatgcaa cgatgagaga ttatcaaaag
    28141 caaggttttc gatggttgaa gatgttaggt cattatcagt ttggcggtat tttagccgat
    28201 gaaatgggac ttgggaaaac actacagaca attgcctttt tactttcgga aaaagaagaa
    28261 cggaaaagct tctctgcttt gatcgtagcg cctgcgagct taatttacaa ctggcaggca
    28321 gaggtgagaa aattcgcccc ttctttaagt atccaagtaa tcaatgggaa taaaaaagaa
    28381 cgtgaagaat tactggctaa agacacagat atccgagtga cttcttatgc cagcttaaga
    28441 caggatctag ctaattatca gtcgcaaaaa attgattatt tgattcttga tgaggcacaa
    28501 atggtcaaaa atagcagtac gaaaactgca caagcattga gagaattagc tgtacctcag
    28561 cggtttgcac tgagtgggac accaatcgag aataatttag aagaattgtg gtcattgttt
    28621 gcaacgatta tgccaggatt tttcccgact aaaacaaaat tcagggaact tgccgcagaa
    28681 gaaattgcgc aaatgattcg accgtttatt ttacggcgag ataaacaaac tgtgttgaaa
    28741 gacttgccgg aaaaaacaga gatgaatcta tattctgcac ttacagaaga acaaaaaact
    28801 gtttatttag cttacttgcg tcaaatgcgt gaagaaatca catcaatgga ttctgaagca
    28861 ttcaagaaaa accggatcgg gatacttgct ggtctgacaa gactgcgaca aatttgctgc
    28921 gatcctcgtc tgtttattga agattaccaa ggcggttccg gcaagctcga acaagtaaaa
    28981 gatctactgg tagctgccaa agagaacaaa cgaagagttt tgctgttttc tcaatttaca
    29041 gggatgctga ctatcttgca agaagagtta gccgagctag ggatctcgac attttattta
    29101 agaggcagta cgaaaccaca agatcggtta agtatggtag acgcatttaa tgctggcgaa
    29161 aaagatgttt ttctgatttc cttaaaggct gggggaactg gattgaatct cacgggagca
    29221 gatacggtca tcttgtatga cctttggtgg aatcctgcca ttgaagaaca agctgctgga
    29281 cgtgcacatc ggatcggaca aaaaaatgtg gtagaagtgt ggcgtatgat ctctgaagga
    29341 acgattgagg aacgcatgga ttcactccag caagaaaaac gcgaactgtt ccaaaaagtg
    29401 atccaaggta atgaagaaca gttgacgaaa ctgactgaag aagacatccg catgatttta
    29461 agtgtaggag aaatcgagga gtaagagact cgaaaaagcg ttcagtaatg ggtaagaaag
    29521 cctttctaaa gaagcgattg tttatttgat ttaaaaaata gcaaggaggc tgggattcca
    29581 ttttatccca gcttccttgc tatcattttt ttatttctat ttttaaaggt tatacaaaga
    29641 ttgtcttcat tgtctctaac agctgatcaa aagaagaaat ctcgatatcg caaattggag
    29701 tctgttcaat tttgcgttct cgatggttga accaaagcgc ttgccagtta gcactttttg
    29761 cacctagaac atcattgtcg taattatcgc caacgtataa ggtctcttct ggaagcatat
    29821 gaaattcttt ctcagctagc tgaaaaatct ctttttctgg tttttggaaa cctgttgctt
    29881 gagaaatgag catattgcgg gaaggaatcc aattgttcaa ttgaagttgc ttcagttttt
    29941 ttgtctgatg gtcagtcggc ccatttgtga taattcctaa tgggacattc ttttccttca
    30001 agaaatcgag ggtttttttg acctcctcat gcaagcagat attatcaagt tcttcctcgt
    30061 aaattttttg aaataagagg ccatcttctt ccgtaatatg agggtagtct aaatctttta
    30121 gtgattggct gatccgatgt gctcgcatat attctagtgt ccaatctcct gccattactt
    30181 ttgggaagtt ttcatcacta tgatggcgga accggatata tagtggatgc atatctgcat
    30241 ctgaaacaag cggaacgact cttttgaccg cgtttcgaaa tggctgctgc tgatcataca
    30301 tcgtatcatc aacatcaaac acaactgatt taatcaaaat tttacacgcc tttcggtttt
    30361 cattgatttt ttcacaaatc cttgactatt gtaacagaaa aaattgtcta aaaaagctga
    30421 taaataaagg tttttcttac tatatttcta tttccttgac agaataaggt ttttatattt
    30481 cagttttttt cactaaaaat cgtgaaacac gtcaataagt ttgatgaaca aattaatcat
    30541 tatggtagaa gtcgattgat ttattttaat gcaatctctg tggaattttc gaaagattta
    30601 tttttttaat acagtctcaa tataactatt caaacataaa ataacgttat ttgatttatt
    30661 tgtttatagt aaattttatt tagtaatcat tgctttaaaa tactacgctt atataacaga
    30721 gaaaataagc gttataataa agaacagtat tatttaaaat gacagaaaac attactatta
    30781 aagaaataaa aaacataaaa aatcttaaga aatcggtttc atttagcttt tgagataaaa
    30841 taatagtaca gttttatacg ctgtttcata tgtttagaaa atttgaatta gatttgaaaa
    30901 ctctaatttt taaatgataa aaaaagcgag aatgctggtt ttactgtata ttaaccttag
    30961 ataactttca tttttttaaa aaaattatga aaaaaaggta gctatttaaa agttgattat
    31021 gtataataga aacagatggg agattattat gatcttctgt tacaaaaaag tgattcttat
    31081 cacgaaaagg agtggtgcac gttattaaca ttatatactt ccccaagttg tacttcctgc
    31141 agaaaggctc gtgcgtggtt gcaagaacat cagattccat ttgttgaacg taatattttt
    31201 tcagaaccat tgaacagttc tgaattaaaa gcgattttac aaatgacaga agacggaaca
    31261 gaagaaatca tctcgacacg ttccaaagta ttccaaaaac tgaacatgga tttagatgat
    31321 ctgccgttac aagaattatt ggaacttgtg caaaacaatc ctggattgct tcgccgtcca
    31381 atcatgattg atgataaacg tctgcaagtt ggttttaatg aagatgaaat tcgtcggttc
    31441 ctaccgcgcg atgtacgaca attagaatta cgtcaagcac aattgatggc tggattatag
    31501 aaggataata cgaaacttgg aaaagactat tacaaagata gtttttccaa gtttttttgt
    31561 ttttacaaaa agtattcttg attagatgta taaaaaggta gtagaagatg atgattgcac
    31621 gaaaaataga gagggaaaat tagctgaaaa gtcggctata tagtattcgt ctgccaaaaa
    31681 tgttatagtt aaataagata gcttgaaagg ttgaaaaatc cgctggtttt attaaattcg
    31741 cttaacagcg agattttttt tctttacatt taagctgatt tcgctacaat gaatctatat
    31801 taaacattgt ggcaaagtga ggtgtagcaa aatggaaatg gaacatatta atgaaaatac
    31861 gatccgtgta ctaattggtc atgaagattt agaggaacgg ggcgtttcat ttttggattt
    31921 acttggcaat cacaaagaaa tcgaaaattt cttttatagt attttggaag aagttgatat
    31981 cgaagaggag ttccgaggaa gcgaagctgt cactttccaa gttttgccaa aaggagacgg
    32041 attagaactg tttatcagta aaaatcttcc ggatgaagaa atttctcaat cagaagattc
    32101 tactgttgat tcagaagatg tgagtgactt tcttcgtcag cagattattg gcagcgatcc
    32161 ggaggaagaa ttatctggaa gcgccctaga agaacaggaa agacaagcaa cttttgcttt
    32221 tgaagatttt gaacaagtga tccaacttgc aacagatgtc gaactagagt ctgcttggac
    32281 tgatttgtat ttattcgaag agcgctatta cctagtcgtt cacttctgga tggaaaatct
    32341 caaccaagcg gatgtagaga atcagattgc tcgaattttg gagtttgctg aaaagagcga
    32401 tcgtacacca gaaacattga atgaatatgg acaatgtctg atggcgcgta atgctatcga
    32461 gcgaacgaaa ttttattttg tataaatcag cattatagta agagactgtg acaaaaattg
    32521 tgtcgcagtc tttttttcgt ataaatgtgt tcaaaagctt gcatctgcgg taacaagccc
    32581 aaccaaacga aaaatattgg gaactatttt tgtttagctg ttcttattac ttgatgcaga
    32641 atagcttttt cacgccctct tttttcgtgt aaataaattc ttagacgtaa ataagttcag
    32701 agggagaaaa atgctgcttg caatgaatga atataataag tgctgttttg ctaaacatgc
    32761 agaaaaagga aagaaatatt attgtcgtgg ctgtcgaaaa agagtgattt tgaaaaaggg
    32821 aaagaaaaaa tgtgctcatt ttgcccatca aaaatcagat aactgttcgg tctttagtga
    32881 aagagaatca aaagagcact tacagttaaa ggaatgcttc atggattggc tgggacagtc
    32941 agtagaacca gtatttttgg aagcctattt gccgaggttg cggcaaaggc cggatatttt
    33001 gttggcgaat ctagcaatag aaatccaatg ttctagactg tctcaccaga gatttattga
    33061 aagaacccaa aattatttga ataacagtta ccaagtatgg tggatacttg gtaattcttt
    33121 tcttggacaa tcgcaattct cattgataga aaaaagttgc tgctactata atagaaaaag
    33181 aggagtgcat tgctggaaag ctgatctcaa gaaacaaaag ctttatttgt atcatcatat
    33241 tacagagact gtatcagggc atatttcctt ttgcagtagc tgctggactt tttctagtag
    33301 ggaccttaaa gaaatattca ctagtaacga aattaaaatg aaccaaatga aaaaaataga
    33361 aaaagtgagt gaagatggga agaaatggct gacacgccaa cttattcaca agcaaaagaa
    33421 tacagtatcc atacaagaac aatgctactt gcgtcataaa catcttttgc atctatcatc
    33481 gtggatctat caaaaaagtc ggtttttctt ttatttacaa gaacaggttt ttctttatcg
    33541 aatgttgtac gaagaagcct tggaaaacca gaaagttcct gatttcaact catggctttg
    33601 ccaaataaaa gaacataaaa gggaatggct ttttcctatg attgatgaag agatggtcta
    33661 tcagcagttt tttaacgaat gcatacactt gtcttctttg aaaaaatagt aagtataaaa
    33721 ataggctagg aatcggtttt ctatgtaaaa caagatttag ttatgctaaa attgatggaa
    33781 gcagacacat gaatgagtag aaaagttaga tgctgtgaca tgtggatgtt agactatgat
    33841 ctattttgat agatagagct aagggaagaa aaggagtgga tttcatggaa gtaaagcagt
    33901 tgccaaaacg agaagaattg cctgaaaatt taacttggga cttgaccaag atcttttcaa
    33961 gcgaccaaga gtttgatgag aaatatttgg aattatcaga agagttaaaa caatctgaaa
    34021 aacacaaagg aacacttgat caaggcgctt ctcaattttt aaatgcgatt gaattcgtat
    34081 tgagggttta tcgccaaact gaagtcattt atgtatatgc gcaccttaaa aacgatcaag
    34141 acactggaaa tacagattac caagcgcttt atgcaagagc aagcagtctg ttttcgaaag
    34201 ttagtgaagc cgtttcctgg tttgaaccag aaatattgca attgtcagat gaccagattt
    34261 ggcaatattt caaagaagaa ccaaaattgg aagtctatcg ccattatatc cagcaaatag
    34321 tagataatcg agcccatgtc ttatctgctg agcaggaatc tcttcttgct ggagcaggtg
    34381 aaatctttga tgcttcaagt gatacatttg ctgttttgaa taatgcagat ctagtttttc
    34441 caacgattga aggagaaaat ggtgaaatag tccaattatc tcatggcgtg tatggtcagt
    34501 tgctagaaag cacggatcga agggtgcgcg aagcagcatt taaggggttg tacagtgttt
    34561 acgaacaatt tagaaataca tttgcttcta ctttaggcac acatataaaa ggacataatt
    34621 ttaaagcgaa agtccgtaat tacagctctg ccagagaagc gtctttgagc aataatcata
    34681 ttcctgaaag tgtatacgat actttggtag acgtggtaaa caagcatttg cctttgttac
    34741 atcgatacat ggaattacgg aaacgtttat tagaagtgga aaaactgcac atgtatgatc
    34801 tttatacacc ggtcttaggg gaagctccaa ttacctttac gtacgaagaa gcaaaagaaa
    34861 aagctttaga agcactgaaa ccaatgggtg aagaatacat ggccatcgta gaaaaagcat
    34921 tctctgaacg ttggatcgat gttgtcgaaa ataaagggaa acgaagcggt gcttattctt
    34981 cgggaagcta tgacacaaat ccatatattt tattgaattg gcatgatacg ctggatcagc
    35041 tatttacgct tgtccacgaa atgggacata gtgttcatag ttatttcact cgttcgaacc
    35101 agccttatgt gtacggcgac tactccatct ttttagcaga aattgcttcg acaacgaatg
    35161 aaaatatcct aacggagtat ttattggaaa cagaaaaaga ccctcgtgta cgggcttatg
    35221 tactcaacca ttacttggat gggtttaaag gaacagtttt ccgccagaca caattcgctg
    35281 aattcgagca tttcatgcat acggaagatg aaaaaggtgt gccattgact agtgaatacc
    35341 taagtgatag ttatggtaaa ttgaatgcaa aatattatgg tccagcagtc gaagaagacc
    35401 cagaaattaa attcgaatgg tcacggatac cgcattttta ttataattac tatgttttcc
    35461 aatactcgac tggcttttct gctgcttcag cactggcgaa gaaaatacta aaccaggaac
    35521 cagaggcact agagaactac ttggcttacc taaaagcagg taacagcgat tatcctgtgg
    35581 aagtaatgaa aaaagcagga gttgatatga cacaggctgc atatattgaa gatgcaatgt
    35641 cgatgtttga acaacgtcta aatgaattag aagaattgat cgatcgttta taaggctttc
    35701 tatttttgat tttctcgaat agaaacgaaa aaaatacgtc ggctctcttg taagggagtc
    35761 gacgtatttt ttta
//
LOCUS       DANJMI010000028        34962 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000028 DANJMI010000000
VERSION     DANJMI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.028"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="PWU81_001636"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="PWU81_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HDL1110340.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="PWU81_001637"
     CDS             complement(2020..2922)
                     /locus_tag="PWU81_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL1110341.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="PWU81_001638"
     CDS             complement(2919..3611)
                     /locus_tag="PWU81_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HDL1110342.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="PWU81_001639"
     CDS             complement(3654..4295)
                     /locus_tag="PWU81_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL1110343.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="PWU81_001640"
     CDS             complement(4333..4647)
                     /locus_tag="PWU81_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110344.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5987)
                     /locus_tag="PWU81_001641"
                     /pseudo
     CDS             complement(4635..5987)
                     /locus_tag="PWU81_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5984..6916)
                     /locus_tag="PWU81_001642"
     CDS             complement(5984..6916)
                     /locus_tag="PWU81_001642"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL1110345.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="PWU81_001643"
     CDS             complement(6913..7689)
                     /locus_tag="PWU81_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDL1110346.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="PWU81_001644"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="PWU81_001644"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL1110347.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="PWU81_001645"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="PWU81_001645"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL1110348.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="PWU81_001646"
     CDS             complement(12014..13294)
                     /locus_tag="PWU81_001646"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDL1110349.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="PWU81_001647"
     CDS             complement(13313..14239)
                     /locus_tag="PWU81_001647"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDL1110350.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="PWU81_001648"
     CDS             complement(14252..15535)
                     /locus_tag="PWU81_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDL1110351.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="PWU81_001649"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="PWU81_001649"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL1110352.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="PWU81_001650"
     CDS             complement(16323..17234)
                     /locus_tag="PWU81_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL1110353.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="PWU81_001651"
     CDS             complement(17231..17695)
                     /locus_tag="PWU81_001651"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL1110354.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="PWU81_001652"
     CDS             complement(17700..18398)
                     /locus_tag="PWU81_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL1110355.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="PWU81_001653"
     CDS             complement(18385..18771)
                     /locus_tag="PWU81_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110356.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="PWU81_001654"
     CDS             complement(18918..20588)
                     /locus_tag="PWU81_001654"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDL1110357.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="PWU81_001655"
     CDS             20771..21004
                     /locus_tag="PWU81_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDL1110358.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="PWU81_001656"
     CDS             complement(21103..21534)
                     /locus_tag="PWU81_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HDL1110359.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="PWU81_001657"
     CDS             21613..22026
                     /locus_tag="PWU81_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDL1110360.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="PWU81_001658"
     CDS             complement(22064..23212)
                     /locus_tag="PWU81_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL1110361.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="PWU81_001659"
     CDS             complement(23334..23930)
                     /locus_tag="PWU81_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL1110362.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="PWU81_001660"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="PWU81_001660"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL1110363.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="PWU81_001661"
     CDS             27160..27591
                     /locus_tag="PWU81_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1110364.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="PWU81_001662"
     CDS             complement(27636..28985)
                     /locus_tag="PWU81_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL1110365.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="PWU81_001663"
     CDS             complement(29055..30218)
                     /locus_tag="PWU81_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL1110366.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="PWU81_001664"
     CDS             30347..30670
                     /locus_tag="PWU81_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110367.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="PWU81_001665"
     CDS             complement(30853..32202)
                     /locus_tag="PWU81_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1110368.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="PWU81_001666"
     CDS             complement(32271..33224)
                     /locus_tag="PWU81_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDL1110369.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="PWU81_001667"
     CDS             complement(33326..34516)
                     /locus_tag="PWU81_001667"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDL1110370.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9952 a         7641 c         6120 g        11249 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatataacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttcttccgaa ta
//
LOCUS       DANJMI010000029        34598 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000029 DANJMI010000000
VERSION     DANJMI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.029"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(27..1259)
                     /locus_tag="PWU81_001668"
     CDS             complement(27..1259)
                     /locus_tag="PWU81_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDL1110371.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1514..2083
                     /locus_tag="PWU81_001669"
     CDS             1514..2083
                     /locus_tag="PWU81_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1110372.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2261..2701)
                     /locus_tag="PWU81_001670"
     CDS             complement(2261..2701)
                     /locus_tag="PWU81_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDL1110373.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2859..3623
                     /gene="map"
                     /locus_tag="PWU81_001671"
     CDS             2859..3623
                     /gene="map"
                     /locus_tag="PWU81_001671"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL1110374.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3655..4578
                     /locus_tag="PWU81_001672"
     CDS             3655..4578
                     /locus_tag="PWU81_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDL1110375.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4803..5042)
                     /locus_tag="PWU81_001673"
     CDS             complement(4803..5042)
                     /locus_tag="PWU81_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1110376.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5047..5466)
                     /locus_tag="PWU81_001674"
     CDS             complement(5047..5466)
                     /locus_tag="PWU81_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL1110377.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5693..6931)
                     /locus_tag="PWU81_001675"
     CDS             complement(5693..6931)
                     /locus_tag="PWU81_001675"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1110378.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7038..7673)
                     /locus_tag="PWU81_001676"
     CDS             complement(7038..7673)
                     /locus_tag="PWU81_001676"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1110379.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(8015..8650)
                     /locus_tag="PWU81_001677"
     CDS             complement(8015..8650)
                     /locus_tag="PWU81_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1110380.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9162..10304
                     /locus_tag="PWU81_001678"
     CDS             9162..10304
                     /locus_tag="PWU81_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL1110381.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10297..10503
                     /locus_tag="PWU81_001679"
     CDS             10297..10503
                     /locus_tag="PWU81_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDL1110382.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10582..11775
                     /locus_tag="PWU81_001680"
     CDS             10582..11775
                     /locus_tag="PWU81_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1110383.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11980..13119
                     /locus_tag="PWU81_001681"
     CDS             11980..13119
                     /locus_tag="PWU81_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDL1110384.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13112..13912
                     /locus_tag="PWU81_001682"
     CDS             13112..13912
                     /locus_tag="PWU81_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL1110385.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13912..14739
                     /locus_tag="PWU81_001683"
     CDS             13912..14739
                     /locus_tag="PWU81_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL1110386.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14717..15451
                     /locus_tag="PWU81_001684"
     CDS             14717..15451
                     /locus_tag="PWU81_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL1110387.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            15551..16417
                     /gene="rfbA"
                     /locus_tag="PWU81_001685"
     CDS             15551..16417
                     /gene="rfbA"
                     /locus_tag="PWU81_001685"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDL1110388.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16431..17003
                     /gene="rfbC"
                     /locus_tag="PWU81_001686"
     CDS             16431..17003
                     /gene="rfbC"
                     /locus_tag="PWU81_001686"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDL1110389.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17025..18053
                     /gene="rfbB"
                     /locus_tag="PWU81_001687"
     CDS             17025..18053
                     /gene="rfbB"
                     /locus_tag="PWU81_001687"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL1110390.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18151..19002
                     /gene="rfbD"
                     /locus_tag="PWU81_001688"
     CDS             18151..19002
                     /gene="rfbD"
                     /locus_tag="PWU81_001688"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDL1110391.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19036..21069
                     /locus_tag="PWU81_001689"
     CDS             19036..21069
                     /locus_tag="PWU81_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110392.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21113..22393
                     /locus_tag="PWU81_001690"
     CDS             21113..22393
                     /locus_tag="PWU81_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDL1110393.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22603..23409
                     /locus_tag="PWU81_001691"
     CDS             22603..23409
                     /locus_tag="PWU81_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1110394.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23421..24641
                     /locus_tag="PWU81_001692"
     CDS             23421..24641
                     /locus_tag="PWU81_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1110395.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24631..26217
                     /locus_tag="PWU81_001693"
     CDS             24631..26217
                     /locus_tag="PWU81_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL1110396.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26256..28394
                     /locus_tag="PWU81_001694"
     CDS             26256..28394
                     /locus_tag="PWU81_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL1110397.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28762..29745)
                     /locus_tag="PWU81_001695"
     CDS             complement(28762..29745)
                     /locus_tag="PWU81_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDL1110398.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30123..31514
                     /locus_tag="PWU81_001696"
     CDS             30123..31514
                     /locus_tag="PWU81_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL1110399.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31530..32570
                     /locus_tag="PWU81_001697"
     CDS             31530..32570
                     /locus_tag="PWU81_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL1110400.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            32589..33674
                     /locus_tag="PWU81_001698"
     CDS             32589..33674
                     /locus_tag="PWU81_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDL1110401.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            33707..>34598
                     /locus_tag="PWU81_001699"
     CDS             33707..>34598
                     /locus_tag="PWU81_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL1110402.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVK"
BASE COUNT        11264 a         5898 c         6786 g        10650 t
ORIGIN      
        1 ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa aatcggtact
       61 gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac catgaaatct
      121 acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga catttctgta
      181 ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga tgcgttttca
      241 tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg gtcaggtaca
      301 agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt tgctaagaca
      361 tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt aaatttcata
      421 cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta accatctact
      481 cgatgactgt caggtgctgt gaacacttct tccgtcggca tatttgccat gaatttttct
      541 ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa tccgataatg
      601 atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt caattcttct
      661 gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc ttcttcatag
      721 acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc ttctggcagt
      781 tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt ccaactgact
      841 ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc tgattgataa
      901 gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga aacaacgctg
      961 atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga ttgtggtaca
     1021 ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg gtcgtcagtc
     1081 cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat caatcgagca
     1141 aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc tgtggctacg
     1201 ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc agataacata
     1261 ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca tttaattatg
     1321 attcaaacaa tgaggcttta aataaaaaaa ggaggttatt tttttaaggt taagtttcat
     1381 gatatatgtt actacttgct atttcagctt gtgaagaaaa tcaaaaacta attttgcata
     1441 tcaaaaaaaa catgttacaa tgaaattaat caatggtgga agcgttataa aaaaattaag
     1501 gagattaaaa aatatggcaa gaaaaaagac gattactaga gaacagattt tggcagctgc
     1561 ttatgaagta gctgccaccg atggatttac acgatttact gcgagaaaca tcgcgaacaa
     1621 aatgaattgt tccacgcaac cgatttatct tgagtttaaa aatatggatg acttgagaca
     1681 agctcttttt gagcagatct atgatcattt acaatatgaa gtgttcactg ttgagcatac
     1741 agggaatacg attatcgatc ttgcttttaa ttatatccat tttgcaagaa gagagaagaa
     1801 gttgtatcgt tcactgtact tagaagaata tggtggagga aaagagatgc agaatttctc
     1861 ttatacttac tttgtgaata cagtgaaacg cgatccagct tatgtgaatc tttcagaaga
     1921 gcagatcgaa tctttgtata atgggacatg gatcatagct actgggatcg cagctttgat
     1981 gtcttcaagc atcatccatc caactgatga acagattgaa aagatgatcc aagacagtat
     2041 agatgctatc ttgaaattca aagaaccaat cgaaatcgat tgaatatact caaaagttcc
     2101 acaaatcagt aggtaacaag agatgtgtca aaataaggaa aagttcgttt ttcttacagc
     2161 gtatgcattt ttaactcccg aagaactctt ccatgaatgc aatgaataaa agagccatac
     2221 agattgaata aatctggatg gctcttttat tgcaagtata ttaaattttt gcgactagtt
     2281 ctttagcgaa agcttctaaa ttttcgatat cttcttcttc agcattcaga tccactttaa
     2341 cattttcggc tccttttgat gcgcctgttt ttgcaaaagc agcatcgaaa tcatccaccg
     2401 atttgcagaa ttcatcgtaa aaagtatcac cagatccgca gacaccgtaa atttttccca
     2461 atagatccaa ttcttgcaag tcttcataaa agtctacgat ctcatcaggc agatcgccat
     2521 catcgtatgt ataagtagct actacgcaaa tatctgcctc ctcgaattca tctgcatcta
     2581 cttgggtaca ttcgttgatt tcgacttcga tatccagatt ttcaaatgct tctgcaacaa
     2641 tatcggcgat ttcttcagtg tttcctgtca tgctagcata tacgattttt gctaaagcca
     2701 tgatgtgcct cctcgtttat aattatttaa caattatgaa ttataccaaa acatcattga
     2761 aaagaaaata aaacctagag acacctgggg aatgtcattt ggctaaatgt atgccgctat
     2821 gttaaaatga gagggatttg taaaaaggag acgaaaatat gattacttta aaatcagcac
     2881 gtgaaattga aatgatggca gaatctggag ctttattagc agatgtacat aaaaatctgc
     2941 gtgattttat aaaaccagga atcacaagtt gggatattga ggtatttgtc agaaactata
     3001 tcgagagtca tggcggaatt gctgcgcaaa ttggctttga agggtatgaa tatgcgactt
     3061 gtatcagtat aaatgatgaa atttgtcatg gattccctcg taaaaagcct ttgaaaaacg
     3121 gtgatttggt aaaagtcgat atgtgtatcg atttgaaagg cggcatttct gattcttgtt
     3181 gggcctatgt agttggagaa tctacacctg agatcgaccg attgatggaa gtaacgaaga
     3241 aagctttgta catcggtatt gagcaagcac aagtagggaa tcggattggt gacatcggtc
     3301 atgctatcca aacttatgta gaaggcgaaa atctagcgat cgttcgtgat tttattggtc
     3361 atggcgttgg tcctacgatt catgaagaac cagcagttcc tcattacgga gaagctggaa
     3421 aagggttgcg gttgaaagaa ggcatggtta tcacgatcga acctatggtt aataccggaa
     3481 cttggaaaat gaaaatggat cctaacggct ggactgcgta tacaagagac ggcggacttt
     3541 cttgccaata tgaacataca ttggctatta ctaaagatgg tccaagaatt ttgacatcac
     3601 aaggagaaga aggcacttat tgatctatcc agtcaatagg tacgggagag gtacatgaaa
     3661 gtttggaaca aaataaaaca aaatgaaaat ctgatgcgct ttattgagac gactcaaaag
     3721 cgcatcactg attcagaaat gagtacgacc tctgtcgtcg ttgcttatta tttactcttg
     3781 tctttatttc cattgattat tgcttttggt aatattttac cgtttctcca catcgatcaa
     3841 gagactgtac tgacctattt acgagaagtg attcctgaga ccgtttacca gtttatcggt
     3901 cctgcaatca aagacctttt gacacaaagt tcaggaggct tgttatccgt ttctgcatta
     3961 gcggctttat ggtctgcaag tcaaagtatc aatgctttgc aaattgcgat gaataaagcg
     4021 tatggcgtag aaaatagaaa aaattttttg atcgtccgtt tcttctcact aattgtcatt
     4081 gtcttgttca tgattgctat tagtggggta actatcgttc ttgggttggg acagctgatt
     4141 ttggatgcta ttcagccaat cgtacaaata cccgtgaact ttatcgatca gttccaaact
     4201 ttgaagtggc caatcacatt ggtagcttta tttgtcatca tgttcttgat ttatttaatt
     4261 gtccctaatg ctcagttgaa gttgaagacg atcattccgg gcacgatctt cgctactgtc
     4321 ggctggatgc tgttatcaca agtgtttagt atctatgcta ggtattttgc aacgaggatc
     4381 agcggttatc agatcatagg aagttttatt gtactcatgc tttggcttaa ttttgctgct
     4441 acgatcatca ttttgggcgg aatcatcaat gcagttgttc aagaatacgt gacgggtcat
     4501 caaatcaaaa aacgtcgaga tccaagccga aaatggttca ataaattgaa aactaaattt
     4561 tctcataaaa aaaagtagaa acaagagttc ttattgtaaa tttatatcag taatttattt
     4621 gaaattttat atctaatttt caattatccg cataaacact atacattcaa gcattttgaa
     4681 gtatagaaaa acactcatct taccacgaat ttaccacgat tgaatgagcc ttgatatgac
     4741 cgtaaaacta ctaagggaga tagcacgaaa ctgtgtatct cccgattttt ttatacagtt
     4801 gattacttca ctccgatttc aaatttcaat attgctttca tcatgtctgt atgaatttct
     4861 ccttttagtt cttcatcaac ctgcataatg attttaccac tttcagagtg gtaaaaagga
     4921 cgcaagcata atttagaaat ataaccgtca taaaacttca aaatctcttt aactgccatt
     4981 tcatctccgc ttgacgcttt gcaaatcaat tcaaaagagg gatagccata atctttactc
     5041 atttctttac ttcctttcgc tcaaaagcat ttttaacttc cttagtcctc tagttcttct
     5101 actgcaaata gcagaacgtg atacatggta cagttctgaa atttctctgt cgttcattcc
     5161 ttgaaaataa cgtaataaaa tcgcactacg ctgtttttct gacagtctat gtaaaactgt
     5221 accaagtgtt tcatcagata cataaattgt aaagtgcaag actttaaatg atacacaatc
     5281 ataaaaacag gtatcaatcg ttccacgttc aacttgtttc gtttcggcta tttcacaaaa
     5341 caatacttca cgtttagaat gtcttgcaag ctgtctgttt cgacttttca ctttgttctt
     5401 aatgacaatc agcattaaag aattgaactg taatctgacg atatgctcga aagaagatgt
     5461 cctcataatc tcaccccctt tctgctaatg ctgaaatata taaacggaat accgtttata
     5521 ccctttagaa atacagaaaa atggtaagaa aaaagcccac acaaaacata tatttgtgcg
     5581 agccttaaat actgcaatat tactctttct tttataacac tatcctttta gcagatttca
     5641 aattttcttt tatatattgt ttttagccta acatactatt taaccccttg tattatatct
     5701 ctaacttttg aaaaattctt tttgaatata ttgaaatcat gattgaagca atagaagcta
     5761 caaacataac aacccatgtt tcttgtataa atacatcgaa taaaataccg taaaccattt
     5821 gtccaatagg ttgaccgcac atagcaactg ctaccagcgt agccattatc tttcctacta
     5881 attgcggtgg tgtttgtgtc tgaacaagag tgtaaatttg aacaacaaac atagtagata
     5941 cacccatagc cacaaagcac atcactgtaa ctacccagta ggaaactatt gcaggcaatt
     6001 ctaaaaatag cgaaattccc ataagtaaaa cagaaacaga acaaataagt aacaaggtat
     6061 aaaggttttt tagttttaac tttgaagcaa caagagctgt taaaatacca ccacataaac
     6121 cacctaatga taatgctgat tgtgaaaaac catatagtgt atctggcatt tttaaaactt
     6181 cagaaattaa gataggcaga ccaacaatta aaacagcact taaaaccaaa ttgaaaatgc
     6241 aaaccaaaaa gattacggat ataaaaatgg gtttctcctt tttcaaaaag gtataactat
     6301 cttttaaatc gtttttagca actgcaatta cacctacttc acgaggtctt ttttggtgtg
     6361 gaatatggat aaaaatttcc ataatggctg aaaaagtaaa acatataata ctaacaatta
     6421 aaatgggatt gataccataa aattgaaaaa ctactccgct tatagctggt ccgataaagt
     6481 ttgctaaagt atttacttga tttattatag cattaccgac ggtaagttta tctgatgata
     6541 ccaatagagg aatacttgct tgtaccgctg gttgatatgc accagaaata ccataaagta
     6601 acatcaaagc aacaataaac aaaggtatga taggaatttt tcccaatgac aaataaaata
     6661 ctaaaattat gattgctgtt acaaaatcta gtatgaccat aatgtttctt ttatttgtcc
     6721 tgtcagcaat aataccacct accagcgaca aaataaccat aggtaaaaaa gagcaggctg
     6781 tagcaatacc aaacaaagct gatgaaccag tttcttttaa caaatagtac ggtaatgcaa
     6841 atcgtaggat agcgtttcca aataaagaaa ttatctgtcc tataacaaca agtataaaat
     6901 cacgattaaa aagttttttt tctgcttgca taaaatctct cctttttatt taatcaaaca
     6961 gaccgtcggt ttatataatg gtttaaataa actgctcttt tatagagcag tctatcgtat
     7021 agggataaca aatacagtta tttacaaaac tgtataaatg cagaaattgt ttcttcgtct
     7081 aataatgtgt ctatgcctat tccatttaaa agggcattga atgtattgca acgtttttcc
     7141 ataccctctt tatccgtcat ttcaactaat gggttgagcc atacattact taataccatg
     7201 ataacttctg caatttcatg tgaattttca atctccaaag acttatcctt gataccttgt
     7261 tctaaaacgg gttcaacaaa ttcaggtact acaatttcat acatttgttc tatttgcata
     7321 gcaagaaaac gaggattgtt taatagttga ggagaaactg tcaataaaac gctttggtta
     7381 gaattgaata ccgctgtctt aaatatttct cttaactttt ctaaaccgtt taaactatcg
     7441 ttatctctaa ttttagcaaa tattactgca ttttcatttc caattctttg aaaaattgct
     7501 tttaaaatat cttcctttga atcgaaatga tgataaatag cacctttaga aagttttgtt
     7561 tcattgataa tatcttgcaa tgatgtgtta tcatatcctt tttcacaaaa catttttgta
     7621 gaaacatcta aaatgagttt tactgtttct tctggatatt tatttcgtgc cataacttac
     7681 ctccaataca aaccgttggt ctgattaaag tatatcttag tattttatgc ttgtcaatat
     7741 aaagacttgc aattttataa aaatgcagaa tgcaagcctt tttcatttat tctacaatct
     7801 tccagttgct ttccttatgc ggtgtcaatt catactgtga tacctgcgtt gcctttgcct
     7861 gatttaccac aaatcagatt ttatcccttt cttcctcttg attgcccaca acagaaaaga
     7921 aaaagccctg tcaagagctt tgccaccatt ggtggtgctt tgcactcttg atcgggcttt
     7981 ttgtttgctg tatcttctcg caagaggaaa aaagttactc gtcctcatca tcaaaattgt
     8041 tattccttaa tacttctcgt aacagttcca tatcttcttt tgagttcggg tttatcattt
     8101 tcacgacttg ataaggtgtc ttatacttat gtccctctat gctttcccca aacatatcca
     8161 tactgtataa atcatcatag cggtctaata atttttgaaa cttcaaatgc tggataaatt
     8221 ctttgattgg gtaaaggtcg gacgcttcaa tgcttttccc gttgatatag tctgtaatat
     8281 attcccgtaa cttttctaac tcatattcca gccattcttc ctcgctgtca aagaagttat
     8341 catagtgtcc atgtttcagt tctgcattta gacgttcttc ggctcttaaa aactgacaga
     8401 cttccttttc agattgattg acatacttcc attctcccga caacaattca ttgggcgtaa
     8461 cgtccagcac ttccattatc ttttccagcg tttcataggc aggatagttc aaccctcttt
     8521 caatcttgga aaggctctgc atattgatac cgattttgtc cgcaagttcc tgttgtttca
     8581 ttcccctaaa ttttcttatg gtctgtatat tatttcctaa atggcttatt ttcattgcat
     8641 aaccctccat atctcgttat atcttcgcta ttataagtct atcatgctta atatgatttg
     8701 taaaggcaaa aaaagatatt gacaaataag catataagaa ttactatgtt cttgtaatcg
     8761 aataagcaca atagaatgaa atataaaaac attcaaaaag aaatacctat ctgattaaat
     8821 ccgtagatat gaatatcggt tgaaatggaa cttgttagaa aaggcaaaac agaacttgct
     8881 gacaaagtat ttctgacacg gtggcggaag cctctcaggc ttaccgccat gacaccttgt
     8941 gtcaagggga agaaatgctt tgtcggctgg cgagcctgcg agccttgtct gtgcccccgt
     9001 tatctaacag gggggcacta tttcacaaaa gacaagtcaa aaaccataca cccccaaacc
     9061 tgtgagtagt aatgagaggt tttaagaaga tagtacatat cacacttttt gaaaggtggt
     9121 ttacaggtat ttcaaggcta ttgtgggatt ggaggaatac aatgaacaat ttagaatttg
     9181 cacttgaaat gaagaacaaa cgtcttgcca gcggtctttc acaaggagaa cttgcaagtc
     9241 ttacccatgt atcaagatat agtatcaacc gctttgagaa cggaaaagct aacgccagca
     9301 aggaaacaca gaacattatc ctgcgttgtc tgaattacta tatctgcgat aagccttttt
     9361 atctgctcgt tgattatctg tctgtccgtt ttccaacgac tgacgcattg gaagttatcc
     9421 gaaaggtgct tggcatgaaa gcagattatt ttatccatta tgactatggg tattatggct
     9481 ataaggagca ttatgcttac ggggaaatca aggtcatggc ttctgatgat gaacacatgg
     9541 gcgtattttt agaattaaaa ggggcaggct cacgcaacat ggaatatgtc ttacaggcac
     9601 agaacaggga ctggtattca ttcttaaacc gctgtcttga ctgtggcggt gttatccgac
     9661 gttttgactt ggcaatcaat gatatgtgcg gtctgctgga tattcctgtg ctatctgaaa
     9721 aatacagaaa tggtggtgcg gaatgtcgct gtaaaaacta tgaaaatgta caaggtggaa
     9781 aattaagcgg aaaaaaccgt aatttagcaa gtacccttta tatcggctca aaggcaagca
     9841 caaaatattt ctgcctgtat gaaaaacaaa aggaacaggc aacgaaaaag aaacataccg
     9901 atattatcaa ccgttttgaa attcgtctgc gtgacaaaaa ggcggtacag gcagttgaag
     9961 aactgttatt gacgtataac cctcatgggc tggtgtttta tctcattacc gattttgtgg
    10021 aatttcctga ctatccattg tgggagatat tcatttccca tgacagttta ccttttgaaa
    10081 tgaaccctgt acctgtcaat atggaacgca ctctgcaatg gctggaaaga caggtcatgc
    10141 cgtcaatcgt gatgatagag gaaatcgaca ggctgacagg ttcaaactac atgaaaatga
    10201 ttgatgaatg tacccacctt tctgaaaaac aggaaatgct tgtggaacag atgtgtacgg
    10261 acatatcaga cgtaatcgaa agtgaggggg tgttttatga gtaatacaca ggatattccc
    10321 gtatgggaaa aatacaccct taccattgaa gaagcgtcaa agtatttccg cattggagaa
    10381 aacaagttaa gacgattggc agaggaaaac aaggacgctg gctggctcat tatgaatggc
    10441 aaccgcatac agattaaacg ccgacagttt gaaaaggtta ttgataaatt ggacgcaatc
    10501 taatgcaaat gagccttgta tgtgttatga tgaacacaag tcatatcaag gctctttcca
    10561 acaaggaaag gagcagacac catgaaagaa aaaagacggg ataacaaagg tcgtatcctg
    10621 catactggag agagccaacg aacagacgga aaatacttat ataaatatgt agacgcattg
    10681 ggaaacacaa aatatgtgta tgcttggaga ttgacaccca cagacccgac accaaaggga
    10741 aaacgggaaa aaccctcact tcgagaactg gaacagcaga taagacggga tattgaggac
    10801 ggtatcgaca gcacaggcaa gaaaatgacg ctttgccaac tctatgccaa acagaacgca
    10861 cagaggacaa atgtgaagaa aagcacacag aaacaacggg aacaactcat gcggttatta
    10921 aaagaggaca agttaggtgc taggagcatt gatacgataa aaccctctga cgctaaagaa
    10981 tgggcgttac gcatgaaaga caaaggcttt tcctacaata ccattaacaa ccataaacgc
    11041 tcgttaaaag cgtcattcta catagccata caagatgatt atgtgaggaa aaaccctttt
    11101 gatttcaagc taagtgaagt catagaaaat gataccaaag agaaagtcgc attgacagag
    11161 gaacaggagc aagccttatt gtcatttatt aagacggaca acgtgtatca caagcattat
    11221 gatgatgtgc tgatactgtt aaagacagga cttcgtatct cggaactgtg cggactgaca
    11281 gtagccgaca ttgatttcaa gaatgaagtt gtgattatcg accaccagtt actaaagagt
    11341 aaggaacagg gctattatat tgaaacgcct aagacgaaaa gcgggataag gcaagtgcca
    11401 ttaagcaaag aaacgataca ggcatttcaa cgggttatca acagccgacc aaagataaag
    11461 tcgatagagg tagacggaca cagcggtttt gtgtttatca atccgaaagg taatccaaaa
    11521 gttgccattg attataacgc cttatttatc cgtatggtaa agaaatacaa caagcaccac
    11581 aaggacaatc ccttgccaca tattacaccg catacgctac gccatacatt ctgcacaagg
    11641 ctggcaagca agaacatgaa cccgaaagat ttacagtata tcatggggca ttcaaacatc
    11701 agtatcacaa tgaactggta cgctcatgcg tccatagata ccgcaaaatc agaggttcag
    11761 cgtctaatcg catagaagta tttaccacga ttttaaccac gattgacagg gaaaatataa
    11821 gaagatagac ctagatatgc gaggtttacc acaaaagcaa aatgcccgta gagccgataa
    11881 aataaggctt tgcggacatt tgagaagata taaaaagata gtcaaaaaga catatataat
    11941 ttataataat tatgactctt aattaaagta ggtgcccata tgcttgtttc ttttagtatg
    12001 gttataagat tttttctgac aattttatta tcactgatat taacacctgt attcaaaatc
    12061 atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa gaagaataaa taaagttcca
    12121 atgccttcag cgggcggact gcctatcttt atttcatttg ttatatcgac tttattctta
    12181 ttccgtaaaa ttatccctca atcgtatatt gtaccgatct tgattgcttc tttagtaatc
    12241 atagtcacag gagtgctcga tgataaatat gagctgacac ctaaacaaaa aagtataggg
    12301 atcttgattg cttctcttat tatttatttt gtagcagata tacgtattga ttccttcacg
    12361 cttcctttta ttggatatat ccaactaggc tggcttagtt ttcccgtaac gattttttgg
    12421 atcttcggta tcacgaatgc ggtcaatctg attgacggtt tggatgggct ggcggcaggg
    12481 atttcattga tcggtttaat tacgatagga atcattggat attttttcct ccatgcttct
    12541 acagtgtata ttccagttgt tattttctgt cttgtagcaa gtattatcgg tttttttcct
    12601 tacaattttt atccggcaaa gatttattta ggcgatacag gagctctatt cctagggttt
    12661 atgatggccg tcctttcact acaagggctg aagaacgtga cttttgtttc atcgatttct
    12721 ttgctgatcg tcatgggagt gccggtaaca gacacgtttt ttgctatcat taggagaaaa
    12781 gcaaaccgtg tttctttttc tacagcagat aaaaaacatc ttcatcaccg actaatcagt
    12841 ttaggcttta ctcataaagg tgctgtcctg acgatttatg cgatggcttt gatgttttct
    12901 ttcacagcta tggtcatgaa ttatactgga agaatcggaa cgatcatatt gattattgcc
    12961 atgttgtttg ctgcaatttt attatttgaa ttgattggtc tgatcaatga gaaacatcag
    13021 tttattttaa aaacattgcg atttttaggg aataaagagt accgtacgca agtaaaacag
    13081 aaatatttga agaaatggaa ccgtcatata gatgaataac aaattaaacc agccgatcct
    13141 tattagtatt gtgacggcaa acagtgataa aatttttcgg acactagata attttatttc
    13201 aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt gacaacaatt cacttcccga
    13261 gtacaaaaaa agactaacag agtataggaa atatccctac atcgatatct attttcatga
    13321 tgaaaattcc ggatttggtt atggacataa tcacaatctg atagctagcg attctcgcta
    13381 tggcattatt tgtaacccag atattttagt ggatgaagag acagttacag cattggtctt
    13441 gttattgaaa gaacatccgg aatgtgcgat gttagcacct aagattctta atgaagatgg
    13501 aacaactcag cacctcatcc gtgagcagct ggctgttttt gattacgttt tgcgctttgt
    13561 gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg gctgtgtttg aatgccgagg
    13621 attgcctact gatgctaata gttatgtacg gatgatctca ggtagtttta tggtagctga
    13681 tattgataaa tttaaagaga taaaggggtt tgatgagcgt ttcttcatgt actttgaaga
    13741 caacgacttg tgtttgagtt ttgaaaaagc aggttacaag ttattgtaca caccctttta
    13801 tgcagttatc catttttacg ggaagggtgc gcatcgaaat gcaaaactgt ttcgcgtgtt
    13861 tttgacttct atgatgaaat ttttcaataa gtgggggtgg cgttttttct gatgaaacca
    13921 gaaatattag tagtcgttgt gctttatcag caactattca gtcaatctcc agcttatgga
    13981 ccattaagca aagcacttag cgaaaagcaa attcagctta ttatttacga taatagtcct
    14041 caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg tttatcatca tgatccgcaa
    14101 aatcctggtt tagcggtggc ttataatttt gcgttgtctc aagcagatga aggaacacgt
    14161 tgtctcgtca ctctggatca ggatacgagg ctagtcgacg attattttga actattgcgt
    14221 aaagtaacct ttacagatga atgtgttgct gcagttccga tgattttttc tgggagccga
    14281 caagtttctc ccctatattc agatcgttat atcaatcgta cagctcaagc agtagaagtt
    14341 gaaggcccaa cagctgaacg aatcatggca atcaattcag gtgttgcctg gtctgtcaaa
    14401 tttctaaaaa agattggcgg gtttaatcct gctttttctt tagatttttt ggatcattgg
    14461 ctgttttgga aagtggatca gttggaaaaa actgtggaga tccttcctgt acgaatggaa
    14521 catgatcttt cggttttaga ctatgaaaaa gtttcaagta atcgataccg ttctatttta
    14581 caagcagaaa atcgatttta tcatgaatat gatcaaaggc atttatccag tcaccgacga
    14641 caattattgt taagaacagc taaacagttc ttgaccgtca aaaaccggca tatctggcgg
    14701 atgactttgc gctcatatgt taagaattgg aaggtgtgaa ttcgtgcgtt cggtttgtat
    14761 agctaccttt aatggaggca gatatttaag agagcagtta gatagtattt tatctcagct
    14821 ttcagcagaa gatgaagtag tcatttctga tgatgggtca gtagatgata catgggatat
    14881 attaactgaa tacgcagaaa aagataagcg aatccagctt ttttcggggc ctcgtcaagg
    14941 attgatcgct aattttggtt atgcgatcca gcagacgaaa ggagatctaa tctttttggc
    15001 agaccaagat gatgtttggt tggagacaaa agtgaaaaag atcactgact attttgagca
    15061 gaatccggat cagctagtag tagtttctga tttaatcatt gtggacagcg agctgcaaga
    15121 gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa ggatggctta ataatgtgat
    15181 aagaagtcac tatattggtg ctggtatggc tttccggtct tctttaaaac ccgatatttt
    15241 gccgatcccg tcagaagtgc cgatgcatga tatgtggatt ggtttgctgg gaggaaagaa
    15301 gactgggttc ctaagagaac cgttaactct gtaccgaaga catgatttaa acgcgagtga
    15361 gattgaaaca cattctaaat tacggcaaaa aatttcttgg cgtgttcatc tgattacggc
    15421 gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg tttaaatcct taatatttcc
    15481 atgacaaata taacaattga tgagataata acaaagtgaa atttgaaatc aaaaaggaga
    15541 gcagatatcc atgaagggaa tcattcttgc tgggggcagc ggaacacgtc tgtacccttt
    15601 aacaaaggca acatcgaaac aattgatgcc gatttatgac aaaccaatga tttattatcc
    15661 aatgtctact ctgatgttgg cgggaatcaa agaaattctg attatttcaa cgccacaaga
    15721 tacaccacgt ttcaaggaat tatttggaaa cggaaatgat ttaggactgc agatcgaata
    15781 tgcagtacaa gaaagtccag atggattggc acaagcgttt atcattggag aagaatttat
    15841 tggagatgat agtgtctgct tggtattagg ggacaatatt tactatggtg gcggtctgtc
    15901 taaaatgttg caacgagcag cttcaaaaga tacaggcgcg acagtatttg gctatcatgt
    15961 caatgatcca gaacgctttg gtgtagtaga atttgatgag aatatgcaag ctttgtcgat
    16021 tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta actggccttt atttttatga
    16081 caatgaggta atttctatcg ctaaaggtat caaaccttca gaacgtggag aactagagat
    16141 tacggatgtc aataaagcat atttggataa aggaaaacta tccgtagaac ttatgggacg
    16201 cggatttgct tggcttgata caggaacaca tgaatcattg cttgaagcat ctacctttat
    16261 cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt ttggaagaaa ttgcttaccg
    16321 tatgggctac atcgataagg aacaattgct ggcccttgca cagccattga aaaaaaatca
    16381 atacggtcaa tatttattga atttggccgc tgaataagga gtccttacag atgaaagtaa
    16441 cagatacaaa attaacagat gtcaaaatcg tagaaatgga cgtctttggc gatcatcgcg
    16501 gtttcttcac agaaagttat tcaaaagcaa aatttgcaga acatggattg aaatatgact
    16561 ttgttcaaga caaccattca ctatcagctg aagctggtgt ccttcggggg cttcatttcc
    16621 aaaaaggaga agctgcacag acaaaattga ttcgggtagt cactggagca gttttagatg
    16681 tagtagtgga tatccgcaaa ggtagcccga cttatggtca atgggacgga tttatcttat
    16741 ctgaacataa tcatcgacag ttgcttgtac ctaaaggata tgcccatgga ttcgttactt
    16801 tgacaccaaa tgtgaacttc ctttataagt gtgataatta ctataatgca gaagcagatg
    16861 ggggcatcgc atttgatgat ccagatttag cgattgattg gccaattgat ccatcaagag
    16921 cgattatgtc ggaaaaagat caccgtcatc cgacattaaa agaatttgaa gcagaaaatc
    16981 catttgttta cggagaaatc taagaattaa ggagaaattt tcttatgaaa aacatcattg
    17041 ttaccggagg tgccggattc atcggctcta atttcgttca ttacgtggta aacaatcatc
    17101 cagaagtgca tgtcactgtt ttggataaac tgacttatgc aggaaacaaa gagaacttgg
    17161 ctggacttcc tagcgatcgc gtagaattgg tcgtaggaga tgttgcagat gcagaattag
    17221 tggaccgtct agtaaaagaa gcggacgcag ttgttcatta cgcggcagaa tcccataacg
    17281 ataattcatt aaaagatcct ttcccatttg tacaaacgaa tatcattggt acgtatacac
    17341 ttttagaagc ttgccgcaag tatgatgtac gctatcatca cgtatctact gacgaggttt
    17401 acggcgatct tccattacgt gaagacctgc caggacatgg ggaaggcgaa ggagaaaaat
    17461 tcactgcgga aacaccatat aatccttcaa gtccatattc ttctacaaaa gctggttcag
    17521 atctgttagt aaaagcatgg gtgcgttctt ttggcttacg tgcaaccatt tcaaactgtt
    17581 caaacaacta tggtccttat cagcatattg aaaaatttat tcctcgccaa gtaacgaata
    17641 tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa gaatgttcgt gactggatcc
    17701 atacgaatga tcattcttca gcagtgtggc tgattttgac gaaaggcaga attggcgaaa
    17761 cctatctgat cggtgcagac ggtgaagaag ataataagac cgtgatggaa ttgatcttag
    17821 aaatgatggg tcaaccaaaa gatgcgtacg accatgtaaa cgaccgtgca ggacacgact
    17881 tgcgttatgc aatcgattcg acaaaattac gggaagagtt aggatggaag cctgagttta
    17941 cgaacttccg tgatgggttg gctgacacga tcaaatggta cgaagaacat gaagactggt
    18001 ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa aaatggacaa taatacagag
    18061 aaaatgtcac tgtattaatg gaaaaacaca tgtgaggcag gcgctcgcct gtctcttttt
    18121 taaaataaaa atcaaggagc ggataatcat atgtttttat taactggagg aaatgggcaa
    18181 ctaggtacag aattgcgcca tttattagat gaaaaaaatc tgcaatatgt ttcaacagat
    18241 gcaaaagact tggatatcac agatgccgag aaaacaatgg cttacatcac agaattaaaa
    18301 cctgatgtta ttttccattg tgcagcttac acagcagtag ataaagcaga agaagaagca
    18361 aaagaattag atgagaagat caatgttgat ggtacaagaa atgtagcact tgcagcaaaa
    18421 gctgtgaatg ctcaattcgt ctatatcagt acagactatg ttttcgatgg aaaaaacaaa
    18481 aatgtagaat acgaagtaga cgaccaaacg aatccactaa atgaatatgg tcgtacgaaa
    18541 ttattaggcg aaaaagcagt agaagagatt ttagaagact attatatcat acgaacttct
    18601 tgggttttcg ggatctatgg acacaatttc gtcttcacga tgcaacgcct agcggaaacc
    18661 cgcgacaaac tgactgtagt aaatgaccag tttgggcgac caacttggac acgtactttg
    18721 gcagaattta tggtatatgt catcgaagaa aaagcaccat ttggtattta tcatttgtcg
    18781 aacgaaaata gctgttcttg gtatgaattc gcaaaagaaa tattgaaaga tacagatgta
    18841 gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag caacacgtcc gcagtattca
    18901 gtcatgagcc tgaaaaagac agaagagtta ggatttgtca taccaacttg gcaagaagca
    18961 cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt aatttccttt tatcaaatag
    19021 aatttggagc atgatatgga aaaacaaaca agaagtgcac gacgaaaaga aaagccaaaa
    19081 gcgaaccagg cagattcgaa tttggtgctt gacctgtttc taaaactccg ctatcttatt
    19141 ggcgttttag tcatcgtggt catcgttact tttaatttaa acggaagttc acttggtgtt
    19201 tgggataaat atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat ttttggacag
    19261 aatcgggaag tgcggtcaga cgaatggctg gtgcagacac ctttttacct ctcacaagct
    19321 gaagaggact ttccgttggt gaacgaggat tattcattaa acggacaaaa catgatcatt
    19381 gcgtataatt ctcctgtaaa ggatattacg gttatcggaa aaccattcaa ctggggattc
    19441 tttttcctcg gaagagatcg aggactttcc ttttattggg cgatgaaatt agtcgtcatg
    19501 gtcttgcttg gattcgaagt actgatgata ttaacgaaga gaaaaaaagc tctatcgcta
    19561 atcggtgcat ttgcgctgac attttctcca gcagtccagt ggtggttcat gcaacatgtg
    19621 ggcgatttga ttttctttac tttgggactg atgactgctt tttatcatta tttctacaat
    19681 catgataaaa aatggctacg aacgttgatg atgcttttgg tggttatttt cggtattggg
    19741 tttattttag tgatttaccc agcccatcaa gtgatgctgg cgtacttgct gattttttat
    19801 tttgttggcc tgcttattta ctatagcaag aaagtttcat gggatctgtt ggatgtagtg
    19861 ttgattggag gggcagtttt atttattggc ggagtcatgg ttcatttctg gctaacgtca
    19921 aaagacgcat tgttagcttc tttgaatacc ttatatccag gaaaacgggt aagtactgga
    19981 ggaaagtgga cgattggcga atttttttct ttccttacaa attggaaaat tccttttgaa
    20041 gatatcaact attcgaataa ctctgaagtg gccctgttct atcacttttt tccaactgta
    20101 ttcctagcaa gtccatttgt gttatttggc aaaaaagata gcgaacaaaa gttactggga
    20161 cgtattttga tgattttttg tctgttttct atcttttgga ttaccgttgg gttgccaaaa
    20221 tggctagcag aaatcactct gctgtcttat gttcctccat taagagccat ccttactttt
    20281 agttttgcgg cttgtttatt atcgatctgg tttatcgctt atatttggag agaagatgtt
    20341 attcctattt ggtataaatt cattttgttg gcagtaaatg catggtctta tttctatgcg
    20401 attacgcatt cgaaaatgga taattatttt tcggattttg aaaccatcgc agttgctgtg
    20461 ttgggaacag tgattttagc ttttgcatta tttggctgga gacgcttgtt ttacctcgct
    20521 tttgccgcct tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga aggtacagga
    20581 gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca agatcctggt
    20641 aaagtttggt tgtctgaaga tgaattatat aacttcaccc caacattggg agtgaaatcc
    20701 ttcaatacag tacgctttta tccagatata gctgcatgga agaaaatcga tccgaaagaa
    20761 gaatacgaga agtattataa tcggtatgct catacaagag cttttatcgc taaggaagat
    20821 acaaagttca ttttagatcg tccagatatg ttttcggtca cgttgaattt tgaagacgca
    20881 gaaaaattag ggatcgacta tgtgatcagt aaacgaccat taactgatta caaccaatta
    20941 tatgaagcgc agttcacgca gctatatgga ccggataaag acggttatat gatttttaaa
    21001 gtagaatatc cggattatcc agatgtacca caaacacaaa ctcaggaaaa tcagctagtc
    21061 gtacagtaat aggctagctg agaataatcc tgaaatgtta gtagaaagga gagtggagat
    21121 aattcaagca atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct tgcttttgtt
    21181 actgacgcta gtcttttttg tttggacagc agggttaggt tttccagcaa ctttgtttat
    21241 atataacaat agttcattac ttatcgcttt tagtgtggct attttgctca ttttaaatgt
    21301 ttataaagca aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc ttttcatttt
    21361 cttttcttac tttcgggaat taagaaatga gtctttattt ggagacttac tgtttccttt
    21421 gattatttta ttttgtttag taattaaatg gatcgagtgg acaaaatggg atcgcggtat
    21481 ttatctgatt gtcttttcgg tcttcctcgg aatcacgtta tatcgtgtat ttacagagat
    21541 ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg atatttggat
    21601 caatacgaat ataatcggaa gttctttgat gactcttgct ttattgatca gtggatttgc
    21661 cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg ttgcagcttt
    21721 tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg tttttgcgat
    21781 attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta ttgcttacac
    21841 ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg aaaaagtcaa
    21901 tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag gagaaaaatc
    21961 tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc aatttttagg
    22021 aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat ttgggattgc
    22081 tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt tatccaacgg
    22141 gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta tggaggatac
    22201 attgacttca tacccatggg ttccgattat ttatatcctg ttggctatgg ccgttcatcg
    22261 atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat atgacgatat
    22321 gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt ccaggatcca
    22381 acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg cagccttttt
    22441 tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga aaatcttgta
    22501 tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat ggtaaaatgg
    22561 cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa agatttgtat
    22621 ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc gatcaatgac
    22681 ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat caatccgcta
    22741 tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca aatgatcgat
    22801 gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt cttcttctca
    22861 gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt aaagaaagtc
    22921 gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta cacccactta
    22981 ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc ttcagcaatg
    23041 tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg attgacttgg
    23101 ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac gatcgtgctg
    23161 cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga ttcaggtctt
    23221 gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg tgagtcattt
    23281 ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg ggttttcacg
    23341 ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca tttttcagat
    23401 gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat atcacaaaat
    23461 cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat ccttttaaga
    23521 aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata aaaaaaggtg
    23581 agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg aaaatcatta
    23641 cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc tcagctttgc
    23701 tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc tatttaaatg
    23761 gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat atcattgaat
    23821 ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc gggatgttcg
    23881 ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt gtggatgaag
    23941 cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt acagaaatca
    24001 agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt cgtcgttttt
    24061 gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat acgatggaag
    24121 taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg gatcggaata
    24181 aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa gcggaattgt
    24241 tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta tacgttaaag
    24301 taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt gcgctacgaa
    24361 cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc aagattcctt
    24421 ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg ttagctggtg
    24481 aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg gtatataaga
    24541 caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc gtggtcttag
    24601 accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat ggacctagat
    24661 gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa agttttagag
    24721 tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca atgtgaagtg
    24781 agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc tcaagatgga
    24841 ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca gacatttgat
    24901 tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac attgaagaaa
    24961 gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa cttggcgcat
    25021 aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta tggtctccta
    25081 gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt tgaaaaagcg
    25141 ggattattca ttaatattga agattatttg tacttagctg taggtgatac agaattaaaa
    25201 tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg accatttggt
    25261 gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag ttccattgca
    25321 gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac gaaacaagat
    25381 gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac actaagtttc
    25441 cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc tagctttatc
    25501 gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa tgctgtagtc
    25561 aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt cgttctaacg
    25621 gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg ggaattgaca
    25681 ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac aaagcagctt
    25741 tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga aaatacaaga
    25801 ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga aagtgatgaa
    25861 tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa aatccaagaa
    25921 aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa aatattaaaa
    25981 atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa gttatcagct
    26041 gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa tcaagcagaa
    26101 cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga gcaagaacgt
    26161 tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga aatgtaaata
    26221 atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa attgccgtat
    26281 ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg ggctgggcaa
    26341 ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat gtgattgctg
    26401 aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat gttaagaccc
    26461 aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc ttagacttcc
    26521 aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac ccttatgatg
    26581 acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg ataaattatg
    26641 caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg aagccaggat
    26701 cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa gagcaaagga
    26761 aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct gtctataacg
    26821 ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg tatgatcatt
    26881 gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag tgtctggaat
    26941 cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat ggacacatca
    27001 gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca ctcttagata
    27061 atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta aacgtacatc
    27121 cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc aatcgctttg
    27181 atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat tatatctctc
    27241 acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt aaaggctatg
    27301 aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct gcagatcata
    27361 tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca actgcaagta
    27421 atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca gatgccctgt
    27481 ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt tatgagattg
    27541 cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag aatggctatg
    27601 aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca aattatgaaa
    27661 tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt gcagaataca
    27721 agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc aattattctc
    27781 gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc ttgaataacg
    27841 atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca cagtttgatc
    27901 gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag catgcaggag
    27961 tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat cgaacacatt
    28021 gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact gcagcttgta
    28081 tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca ttagaagtag
    28141 cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac aatgtctatg
    28201 cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa gatactccag
    28261 aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg ccagcatata
    28321 ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc gacttttcgt
    28381 tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg cacataacgg
    28441 ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa ataagtcgag
    28501 gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa ttgcttttca
    28561 aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac cgtttattcg
    28621 tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta ttcatgctca
    28681 gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg tcaaagaatc
    28741 gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt cctctacaaa
    28801 agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa ccagattgtt
    28861 cttagcgtca atataaagaa catttccact attaccaatt gctgaatata tttcgttctc
    28921 agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt ctaaagcact
    28981 ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt ttcctttata
    29041 catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg ataaacacaa
    29101 accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg ttttaggaga
    29161 cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat tgacatggtt
    29221 tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg ttagcttact
    29281 tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt tgaattcgcc
    29341 ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat cttcacttga
    29401 acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg ttagcaagtg
    29461 cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact ctggaaaata
    29521 atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa tgccaatcag
    29581 caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt ttttataacc
    29641 attccattca tctgtataaa cttctacccc atccttatat gcgacaattt gacatatatt
    29701 tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat attacctcac
    29761 tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat tcttcacgta
    29821 atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct gaaaaatata
    29881 aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct ttaatttacc
    29941 aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg tcttgattag
    30001 aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc ttataaacta
    30061 ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgtgatt aggagtgtat
    30121 atatgaataa aaatggagaa tggagtgctg cacgacggat attcattata ttaatggatg
    30181 ttattgtata caatgcagca atatatagta gtttcttact gaagtttcgt ggagagatcc
    30241 cattacgaaa tttagaaact ttccaacact cagcaattat tatttcagtg atttttattg
    30301 cgttgaacat tttgttgggt gcatatgttt tttataatcg aatgatcagt gacatcgtgt
    30361 ttgtcacagt catcggtcag gtattgatga cactagggat catggtagtg acttttgcgg
    30421 gccgctggtt tgcttttcca cgtcttgtta tcttactttc atttgttctc ggtacgcttt
    30481 tcttaagtat ctatcgtatt ctcgtatata aactttattt acgcgtaagt cgagataaaa
    30541 aggtcgtcat tattgggtta gaaaaggatg tcgcaccagc aatccaaaat tttggttcaa
    30601 agaaaaacaa caaacataaa gtcgaagcag tcattcttga taactattat gcaaacatca
    30661 aaaatatgat taaagacatc gatattgtct atcttgcttc tcatatcgaa gaaactgaaa
    30721 aaatcaaaat ctatcaatta gttactaaaa atgaaaaaaa attgtttttg aatacaacat
    30781 ttgaaaactt gacattgatc aatccaaata ttatgaattt cgaagatgaa agtattattg
    30841 aagcatctgg atttagaatc caacctgaat atgaactatt caaacgtttg tttgatatca
    30901 tcgtttcact tgttttatta gttattgctt caccatttat gttattcaca gcaattttag
    30961 tcaaagtgac atcaccggga ccaatcatct ataaacaagt gcggatcaca aaaaatcaaa
    31021 aggaattttc aatctataaa ttccgctcga tgaccgccac agccgaagca aaatctggac
    31081 cagttttagc taagagcaat gatgcacgag taacgcctgt aggtaaattt attcgagctg
    31141 tccgctttga tgaattacca caaatcttca atgtgctgaa aggcgatatg tcgatcgtag
    31201 gaccacgtcc tgaacgtcct ttcttcgtcg atcagttcaa tgcagaaaac ccttattact
    31261 acttacggca taatgttcgt gctggtatca ctgggtatgc acaagtctat ggaaaatatg
    31321 tctctgatta caacagtaaa ttacgttttg atttacttta tatcaaaaaa tattcattga
    31381 tgcttgattt aaagatcttg ctgcaaacca tcaagatcct atttgataaa atgtcctctc
    31441 aaggacttga agaagacgaa aatgtgatct cattggcaga aattgctttt ccgaatgaaa
    31501 aaatctttcg ctagttggga ggcgtaaaaa tgaacaatcc gaaattcagc ataatcgtac
    31561 cagtatacaa tgcagaagca actattggtc gaacgattga tattctaaaa gaattggatt
    31621 atgaagagta cgaagtcgtt ttagtaaatg atggatcaac agatcaaaca caggaaatca
    31681 ttcaagaact gattgaaaat gatgaacgtt ttgaattgat tactacgacc aatcaagggc
    31741 ccggattagc aagaaacgaa ggaataagac agtcaaaagg agaatatctc ttattcttcg
    31801 atgcagatga cacgccagta aaaacgatct tgtcagatta tgatcatttg ctagaaaaga
    31861 gtccaaaagc ggatcttgtt gttagttcgt ttgtttttcg tacaatggac gagggaagaa
    31921 tcgtttccga aaaagaaaac ttagttacag aatgtcacta tctaactcac gaagagttta
    31981 taaaagacat gtatcagtta atgaatgaac agttgatgta tgttgtatgg aacaaatgct
    32041 atcgtagcga tattctgaaa aataaccaaa ttctatttaa aacttataat agttgtgagg
    32101 atcgaatatt taacttacat tattatgaat actgtcatca agtgatcatg aacccaaaag
    32161 tagaatatat ttatgaattt gaaggtggga aggggatcac taatcaatac cgacctaaca
    32221 agttcgaaac atttaaagaa ttctattcat tagcaaatac tgtaacagac gatattaata
    32281 aagacggtat ggcggcgctt cttttaaaag ggacaacttc agttattttt tctatttata
    32341 gtacctctgc aagaacttca aaagagaaga aagacgaagc aaagcagata ttgagtgatc
    32401 cagtgatccg agaagccaag caaattgcta agacggattc ttcagcaaaa aaaataacaa
    32461 agttgcttta caatatgcca gctccactat ttttatctgc tgtaaaagtt ggaagcttcg
    32521 ttgaagtgaa aatgccaggg ttaatggctg ctttgaagcg agtatattag ttaatggagg
    32581 aaaatataat gaaaagaatt ttgcattttc agggacgaat gggtttaggt ggagcagagt
    32641 ctttcatgat gaatttatat cgtaaaattg atcgtacaaa ctatcaattt gattttttga
    32701 tttatgaaga ttatgcagat gttcaagact atcattcaga gattgaaaga ttaggaggcc
    32761 gtatttttgt tgtacctaat cctaagaaaa atatttttaa atatttaata gaagtgaata
    32821 agttactaaa aaaagaatct ttttctatag ttcacaatca ggtttatttt ggcgggggaa
    32881 ttaatttatg gttagccaaa aagaatggaa tcagacagag aattgctcat agccatgcaa
    32941 ctgaagatgg aaaatctcaa aatattgtaa tgaatgtatt gcgtaaattt ttaaccaaat
    33001 tgttgttaca aaatgcaaca gactaccttg ccgttagtca agaagctggt gaaagtctgt
    33061 ttcaaaacca cccctttgag atagttcata atggtattga tttagagctt tatagtaaaa
    33121 attcagaagc taaggttaat aagagaaaag aattggatat ctcaatgagt acttttgttg
    33181 tgggaaacat tggacgatta gagaaacaaa aaaatcaatc ttatttgtta gagatttttt
    33241 tagaaatact aaaaatgaat tctgattctt acttgatttt agtaggtgaa gggtcactta
    33301 gaaataaact gaaagccaaa gcagtagaat tggatataga gaaaagagta aaatttttag
    33361 gagaaagaaa cgatattccc gagttattat ctgtgatgga tgtttttgta atgccttctc
    33421 tttatgaagg tctgccaatg gttggattag aagcacaagc agcaaaatta aaattagtct
    33481 tagcagatac tatttccaaa gatacaaagt taacaaataa tgtggatttt gtatcattag
    33541 atgaaacacc acaagaatgg gcaaaaaaag taataaaaga gccgtatgct aataaaataa
    33601 cacaagaact aaagagttat gatgtttcgt acactactga acaaatggag agtatatata
    33661 gtagagatca ttaaaaatgt tcaagatatt ttaggagtga ttatttttga aaatatgtta
    33721 tttgatattg gcatacaatg atattggcaa cttaaagaga ttggtcaatc gattgaacga
    33781 aaatgcagtt tttattattc atatagataa aaaaactgat atagaaccat ttattgaagc
    33841 atttaaatca tatgataatg ttgaattttt atgtaataaa aaccgtgtta aaatttattg
    33901 gggtggtttt tccattgtac aagcagaaat caatttagta aagagagcat tacaaaatga
    33961 gaaatattta aaatatgtat tattatctgg tgctgattat ccaatcaaag ataatgagta
    34021 tatttataat tattttaaaa agaatagttc agtggagttt attcgtggaa tagatctaga
    34081 tcaaattaaa cataaagaat tttattataa acatatagat gtatatcaaa aacatgatta
    34141 tcctagaatt aataggaata acacaactgc atttaaaatt tttagagcta taattaatcg
    34201 ttgtctgagg atgattaaac ttcccccaaa aattagacac cataaatttg atttgtatca
    34261 tggttctcaa tggtgggctt tgtcgaaaga atgcctaaca gaattaatac aaatgtatga
    34321 acaaaatcaa caagactacc tgaattttaa aataggtatg tttgccccag atgaaaaatt
    34381 ttttcataca ctttttttta attcctcgtt caaaaataaa aatgttattg gaggaccaga
    34441 tttaccactt gaattgaaaa atattgaaga aactacttta caaacttcga aattagcaaa
    34501 tattcatatt attgatcctt ccatgaacaa atggtttgat gaaagggata tagatcaaat
    34561 aaaagaaagt gaaaaattat ttgtaagaaa agttaaat
//
LOCUS       DANJMI010000030        34528 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000030 DANJMI010000000
VERSION     DANJMI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.030"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(61..2424)
                     /locus_tag="PWU81_001700"
     CDS             complement(61..2424)
                     /locus_tag="PWU81_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HDL1110403.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2872..3093
                     /locus_tag="PWU81_001701"
     CDS             2872..3093
                     /locus_tag="PWU81_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1110404.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="PWU81_001702"
     CDS             complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="PWU81_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL1110405.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(3575..5881)
                     /gene="infB"
                     /locus_tag="PWU81_001703"
     CDS             complement(3575..5881)
                     /gene="infB"
                     /locus_tag="PWU81_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL1110406.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(5894..6205)
                     /locus_tag="PWU81_001704"
     CDS             complement(5894..6205)
                     /locus_tag="PWU81_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HDL1110407.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(6202..6495)
                     /locus_tag="PWU81_001705"
     CDS             complement(6202..6495)
                     /locus_tag="PWU81_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDL1110408.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="PWU81_001706"
     CDS             complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="PWU81_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL1110409.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="PWU81_001707"
     CDS             complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="PWU81_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL1110410.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(8333..12685)
                     /locus_tag="PWU81_001708"
     CDS             complement(8333..12685)
                     /locus_tag="PWU81_001708"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDL1110411.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(12897..14606)
                     /locus_tag="PWU81_001709"
     CDS             complement(12897..14606)
                     /locus_tag="PWU81_001709"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL1110412.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="PWU81_001710"
     CDS             complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="PWU81_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL1110413.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(16103..16903)
                     /locus_tag="PWU81_001711"
     CDS             complement(16103..16903)
                     /locus_tag="PWU81_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL1110414.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(16900..17712)
                     /locus_tag="PWU81_001712"
     CDS             complement(16900..17712)
                     /locus_tag="PWU81_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDL1110415.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(17907..18464)
                     /gene="frr"
                     /locus_tag="PWU81_001713"
     CDS             complement(17907..18464)
                     /gene="frr"
                     /locus_tag="PWU81_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL1110416.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(18467..19189)
                     /locus_tag="PWU81_001714"
     CDS             complement(18467..19189)
                     /locus_tag="PWU81_001714"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL1110417.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(19325..20206)
                     /locus_tag="PWU81_001715"
     CDS             complement(19325..20206)
                     /locus_tag="PWU81_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL1110418.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIEYDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="PWU81_001716"
     CDS             complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="PWU81_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL1110419.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            21446..21925
                     /locus_tag="PWU81_001717"
     CDS             21446..21925
                     /locus_tag="PWU81_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDL1110420.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            22142..23233
                     /gene="phnW"
                     /locus_tag="PWU81_001718"
                     /pseudo
     CDS             22142..23233
                     /gene="phnW"
                     /locus_tag="PWU81_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            23226..24011
                     /locus_tag="PWU81_001719"
     CDS             23226..24011
                     /locus_tag="PWU81_001719"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL1110421.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(24357..26048)
                     /locus_tag="PWU81_001720"
     CDS             complement(24357..26048)
                     /locus_tag="PWU81_001720"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL1110422.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="PWU81_001721"
     CDS             complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="PWU81_001721"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDL1110423.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(27532..28551)
                     /gene="argF"
                     /locus_tag="PWU81_001722"
     CDS             complement(27532..28551)
                     /gene="argF"
                     /locus_tag="PWU81_001722"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL1110424.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="PWU81_001723"
     CDS             complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="PWU81_001723"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDL1110425.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            30332..31033
                     /locus_tag="PWU81_001724"
     CDS             30332..31033
                     /locus_tag="PWU81_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL1110426.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(31605..32621)
                     /gene="tsaD"
                     /locus_tag="PWU81_001725"
     CDS             complement(31605..32621)
                     /gene="tsaD"
                     /locus_tag="PWU81_001725"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL1110427.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(32618..33082)
                     /gene="rimI"
                     /locus_tag="PWU81_001726"
     CDS             complement(32618..33082)
                     /gene="rimI"
                     /locus_tag="PWU81_001726"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL1110428.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(33089..33631)
                     /gene="rimI"
                     /locus_tag="PWU81_001727"
     CDS             complement(33089..33631)
                     /gene="rimI"
                     /locus_tag="PWU81_001727"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL1110429.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(33615..34337)
                     /gene="tsaB"
                     /locus_tag="PWU81_001728"
     CDS             complement(33615..34337)
                     /gene="tsaB"
                     /locus_tag="PWU81_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL1110430.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         9362 a         7392 c         6099 g        11675 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca
     3181 ggatttgcgg gattttttga ttagtctttg ttcaagtcgc gaagcaattc atcgatatgg
     3241 ttcccataga caactgattc atcaagttca aagaacaatt caggtgtctt ataaatactt
     3301 agattgtgcc ctaactctcg acgaatcaat cctgatgctt tattcaatcc atcttgtgct
     3361 tttttcttat ctgatgctaa gtcagataaa atgctgtaat agatcgttgc ttgctgtaag
     3421 tcccccgtaa ctttcacatc tgtgatcgtt acgttctcca cacgagggtc acggaccttt
     3481 ttgcgcaaaa tatcattgac ttcttttaag atttcttgac cgactcggcg atcacggtag
     3541 ttagccataa tgattggcct ccttttatca gtttttagtc atttttgact tcttccatga
     3601 taaatccttc aattacgtca tctactttga tgtcattgaa tttttcaatc atcgcaccac
     3661 attcataacc cattttgact tctttcacgt catctttgaa tcgtttcaga cttgcaagtt
     3721 ggccttcata gattacgatt ccgtcacgga ttacacggac accgctatct ctacgaatat
     3781 atccttctgt tacataggca cctgcaatcg taccaacttt agaaactttg aaggtttcac
     3841 gaacgatcat ttgaccggtg attttttctt caaattcagg gtctagcatc cctttcatcg
     3901 cagtttcgat ttcttcgatg gctttataga tgatacggtg aagacgaata tcgacttctt
     3961 cagtatcagc ttgcactttt gcctgcggtg taggacgaac gttgaaaccg atgataatag
     4021 cgttgctggc tgctgctaag gttacatcac tttcattgat tgctccgaca gctgaatgga
     4081 cgatttttac acggacacct tccacatcaa tcttcttcaa tgatgcagcc agtgcttctg
     4141 ctgaaccttg tacatctgct ttgatgatta cgttaacttc tttcaattcg ccttctttca
     4201 ggctttcaaa taagttgtcc aaagtcacac ggcttgtggc agcacggttt tccaataatg
     4261 cacgttttgc gcgttcttca cccgctgcac gtgctgtctt ttcgtcttcg aagactacga
     4321 aacggtcacc tgcttgaggt acatcattca agccagtgat ttctactggc gttgcaggtc
     4381 ctgcttcttt atcgcgacgg ccaagatcgt ttgtcattac acggacacgt ccgtaagtat
     4441 taccgacaac gattgggtca ccaacgtgaa gtgttccttc ttggacaagt aaggttgaca
     4501 caggaccttt acctttatca agacgtgctt cgatcactgt tccgattgcg cgttgtgttg
     4561 gatctgcttt taaatcttct acttctgcga ctaacaggat catttccagc aattcttcga
     4621 tgttttggcc gaatttcgct gagatctcaa caaaaatcgt ttctccaccc catgcttcag
     4681 gaattaattc atactcactt aattcttgca taacatgctg aggatttgca cctggtttat
     4741 caattttatt tacagcgacg atgattggaa cgcctgcagc tttggcatgg ttgattgctt
     4801 caactgtttg tggcatgacg ccatcatctg cagcaacaac taagatcgtg atatctgtga
     4861 tacttgctcc acgtgcacgc atacttgtaa aggccgcatg tcctggtgta tccaagaaag
     4921 tgattggttt accatcaata tcgatctggt aggcaccgat atgctgtgtg attccgccgg
     4981 cttcgccgct tgttacacgt gaatggcgca atgtatccaa taaggttgtt ttcccgtggt
     5041 ctacgtgtcc cataatcgtt actactggcg gacgtgagac taatttatct ggatcgactt
     5101 cttctgcctc gaagaattta tcgatatcag caatgtcaac ttggactttt tcttgtggtt
     5161 ccatgccata gtccgtcgct aacagttcaa tcgtatcttt atccagtgct tggttttgat
     5221 tgaccatgac accaagcata aataattttt taatgatttc tgctggctca cgatgaattt
     5281 ttttcgcaat atctgctacg ttcatacctt cagtatactc taatacatct ggcaattcac
     5341 ggaatttgcg tggtggtaca gctggtttag ttgattgttg ctgtttgcct tttttacctt
     5401 ttttattata acgattacgg ttattattat taaatttgcc gcgattgtta ttgtttggtt
     5461 tgttgttatt gttagtacgg ttttgattgc cgccttggct cgttccttga cgattttggc
     5521 tgctggtatt tcccgtatta ttttggtttg ttgttggttt ttttccttga ttcacttgac
     5581 cgctcccttg accgcgatct tgataactgc ggttattttt atttttttgt tgattgttcg
     5641 ttgtttcttt tttacgttga tcgttcggct gttgattggc tggttttctg cttgcttgtt
     5701 gtgcagcttg ttgtttcgtg cttgggtttt tgaacgcttg ttgcaatttc ttctcatcac
     5761 tgctagtaat cgcacccata tggtttttca catcgatgcc aagttgttgt gctttttcca
     5821 cgacgtcttt actagatttt ttgagttctt ttgctaattc atagattctt ttgttgccca
     5881 tgcaatcacc ttcctaacct tcaattagct ccttggtctt cttcgcaaat ccggcatcca
     5941 ttattccgaa taccttgcga ggttttccga tcatctgcgt gatttctgct tcagaaaata
     6001 gctcaaagca aggaacttca taatatgaac tcttatcttt tatctttttt cttgtattct
     6061 cacttgcatc ggttgccaca aacaccaatt ttgctttttg tttgcggatt tcagcaatgg
     6121 tcagttcttc acccgtgatt agttttcccg cgcgcatcgc tagaccgatg agattcatgg
     6181 ctttttgccg attaactgta ttcattttcc aaataactct ttacgtgctt tttggtgagt
     6241 gacatagtca agaagttctt gataaaactc atctgttaat gttgtttcga gtactcgatc
     6301 gagaattttt ttatcccaag cttgttgtac ttcttccggc tcaagtgaga catacgctcc
     6361 tcgtccaggc atttttccag ttggatcgat cgatacttcc ccttcttttg aacgggtgat
     6421 tcggaccatc tcttttttag gtttcatttc acctgaaacc acggatttgc gtaaaggaat
     6481 tttcctttgt ttcatctctt tgcctcctta ctaaagttaa gcttctggtt tttcttcgac
     6541 cgtttcattg ttgaatgcaa ttgtttcata ttcatcgtct gttaaatctg actgaacgat
     6601 tgcttcatca tgcagttctt ctgtttcttt ttgttgttct tgattttcat aaaattccgt
     6661 catgtcagat tctgatttga tatcgatttt atggttagtc aatttagcag ctaaacgtgc
     6721 attttgtccg cgtttaccga ttgctagcga taattggtag tctggaacga caactgtgca
     6781 ggcctttgga tttgtctcat cgaagatcac atccatgact tgtgcaggat tcaatgcatt
     6841 tgcgatgaat acagctggat cttcattcca ttcgacgata tccatgtttt cgccttttaa
     6901 ttcattgacg attgcttgaa ctcgttgtcc ctttggcccg acacatgtac cgactgcatc
     6961 gatatttgga tcatttgagc gaactgccac ttttgaacga tcgccagctt cacgggcgat
     7021 gctgacaatt tcaacgattc catcatagac ttctggtact tcttgttcaa ataagcgacg
     7081 caataggtct ggatggctgc ggctaacaaa tacttgcgga cctttagacg tattttctac
     7141 tcgggaaaca taaactttga ttcgatcatg tggttgataa aattcatttg gcatttgatc
     7201 ttgtttagaa agaactgcct caattttacc aagattgaca taaatataac gtttatcctg
     7261 gcgttcgacg attccttgca tgatgtcttt ttcataagca ctgaattcgt tataaataat
     7321 cgttctttct gcttcacgta cacgctgtaa aataacttgt ttagccgttt gcgcagcgat
     7381 ccgtccaaaa tcttttggtg ttacttcaaa acggatcgta tcaccgatct catatgctgg
     7441 attgattaaa agtgcatctt tcaatgagac ttccaactgt gaatccatca cttcttccgt
     7501 tacttctttt accgcataga catggatctt tccttttttc tgatcaaatt ccacttcgac
     7561 gttttgtgct tggccatagt gacgtttgta tgcagaaacc aatgctgctt ctaaagcatc
     7621 gatcacgatt tcttttgaga tccctttttc agcttctaaa gcgtctaacg catttaacat
     7681 ttctttactc atttttttct gattcctctc cgtgattaaa attggatagc taagcgagct
     7741 tttgcaatat tttttcggtc aaaagtcaat tctttttctc gtgtttttat gcgtattttc
     7801 aatgtaagct gttcagcatc aaatgattga agaaatcctt catattgttt ttcgccatct
     7861 actggttggt ataaggagac atggacataa tcaccaagtg ccttttcata gtcgctttct
     7921 tttttcaatg gccgctctgc tcccggggag gatacctcca ggaaataagc ttgaggaatc
     7981 ggatctggat ctgctgcatc aagtttttca ctcagtttct cactgacaaa ggcgcattct
     8041 tcgatatcga taccgccttc tttgtcaata aatactcgta agaaccagct ttttccttct
     8101 tttacaaatt cgacttcgac aagttcaaaa ttttgttcat ctaaaatcgg tgtcaccatt
     8161 tctgtgactg tttcaacgac gctactcaaa agtttcgcct ccttaaaaat taagtgcgta
     8221 attccattaa aaagagtgag cgaaaaatcc gctcactcac agttagtatc attacctata
     8281 agaactatac catactctgc ttaaaattac aagcagacag gaaaacagat gcttatagca
     8341 tatcgaataa cgataattga ttttcatctg gtagatcttt caatacgcca ttgtcgttca
     8401 tatattcgat caatgttttt gaaactttac cgcgggtggc aagatcttct ttggataaaa
     8461 atggaccgtc ttttcttgct tcaacgattt gtttcgccac gttggttccc aaacttggga
     8521 cagctctaaa tggtgcgatc aacgtatcac cttcaataac aaagttaacg gcatccgatt
     8581 tatacaaatc aatcattcca aatttgaatc ctcgttccag catttcattg gctaactcca
     8641 gcactgtcag ttgattcttt tcttttacgg aagcatctaa tcccttgtcc gtgatttctt
     8701 tcattgcttg tttcacagca tcttttcctt tgcacatgga gactagatca aagtcgtctg
     8761 cacgtacaga aaagtatgca cagtaataca aaatagggaa ataaaccttg aaataagcga
     8821 cacgaagagc catcaatacg taagctgcag catgggcttt cggaaacata tatttgattt
     8881 ttgaacaaga atcgatatac cagtcgggca cattattttc tttcatcgcc gatagatagg
     8941 tttctcgcaa ttcatcaggg atcttgttcc attgtccttt acgaacagtt tccatgattt
     9001 taaatgccat cccgctatct aatccagcat ggatcaaata aaccataata tcatcacgac
     9061 agccgattac ttccgccagt gtcgcatctc ctcgtcgaat caattcttcc gcatttccta
     9121 accatacgtc tgtaccatga gatagtccgg aaatttgcag tagttctgca aaagttgtag
     9181 gatgcgtttc ttctaacatc cctcgaacaa aacgagtgcc gaattctgga atacctaatg
     9241 tccctgtttt ggaataaatt tgttcctgac ttactccaag tacttcagga ccggcaaata
     9301 tccgcatcac ttccgggtca tccgtaggga tcgtttgtgg gtcgatccct gataaatctt
     9361 gaagcatccg aataacagtt gggtcatcat gccctagaat atctaatttc aatacattat
     9421 cgtgaatcga gtggaaatca aaatgagtcg ttttccattc tgaattttga tcgtctgctg
     9481 gatattggat cggcgtaaaa tcatagacat ccatataatc gggaataacg atgatccccc
     9541 ctggatgctg gccggttgtc cgtttgactc cagtagcgcc ttttgctaat ctgtcgactt
     9601 ctgcacttcg aaactgcaga ttattgtccc tttcgtatcc ttttacatag ccatatgctg
     9661 ttttgtcggc gactgtacca atcgttccgg cacggtaaac atagtcttcg ccaaacaata
     9721 ctttcgtata gttatgggct tctgcttgat aatcacctga gaagttcaaa tcaatatcgg
     9781 gcactttgtc tccatggaat cctaaaaatg tctcaaatgg aatatcatga ccgtctttgt
     9841 ttaagcgtgc accacatttt ggacattgtt tttctggcat gtcaaatcca gaaccatacg
     9901 taccatcttc aaaaaactcg gaatattggc actctggaca ataataatga ggggctaatg
     9961 gattaacttc agtgattcct gtcatcgtcg caacaaagct agagccaaca gagccacgtg
    10021 aacctactag atagccatct tcattacttt tatgcactag tttttgggcg atcagataaa
    10081 taaccgagaa gccattacca ttaatagaat tcaactcttt ttctaatcgt ttttgaatga
    10141 tctcaggtaa cggatctccg tacatttgtt ttgcttttgt gtaactcaat tctgatattt
    10201 catcttctga acctggaatc ttcggtgtat aaagttcatc tttgactgga atcacttctt
    10261 cgcagatatc tgcgatttta ttcgtatttt ctactacgat ctctttcgct gtttcttccc
    10321 ctaaaaagtg aaatgcagtt agcatctcat cggttgttcg gaaatggaca tccggcaaac
    10381 tatgacgatt caacggattc gctccgccca tagaattgat caaaattttt cggtaaatgg
    10441 catcttcttc gttcagataa tgtacattac ctgttgcaac aacgatttta tccaaacttt
    10501 ttccgatttc taccaaattt tggataattt cttccaaatc atgttcattt tttaccaatt
    10561 cttgctcgat caaaggcgca tagaccgctt ttggcataac ttcaatataa tcatagaatt
    10621 ttgctcggtt tcttgcctct tctacccctt tttgcatcat ggcttcgaat atttctccct
    10681 tatcacaagc tgagccgatc aatagatttt cacgcatctt tttcaattgg gagcgtggta
    10741 ttcgtgggac gcgttcgaag tattcgacat tcgacattga aatcagtttg aacaaatctt
    10801 tcagtcctgc ctgatttttt gctaaaatcg ttacatggaa aggtcgagcc cttttgtaag
    10861 aatctccttc tccgatatgc tcatttagct gatcatgata aagcatgtta tgattgtcca
    10921 ttgcctcttt cacaaagatc catgctaagt gaccagttgc ttcagcatca tagattgctc
    10981 ggtgatgctg ttccaagcta acaccaaact ttttagataa cacacctaat ccgaaacgtt
    11041 tgaattgagg atacagatat ctagctaatt ccaacgtatc aatgactgga ttggccgctt
    11101 caggaatacc ataccttgcg tagctagtat tcaagaaccc catatcaaaa gccgcgttgt
    11161 gggctaccaa aattgtatct tttgaaaatt caaggaacat ccgcaatact tcttcttccg
    11221 actttgaccc tcgaaccatt tcatccgtga taccagtcag atctaccgtt gttcgtgaca
    11281 atggatgtcc tggatcgata aattcatcaa agctttcgat cacatttcct ttatacattt
    11341 tgactgcggc tagctcgatg atcgtgtcat atactgcaga caatcctgtc gtttccacgt
    11401 caaatacgac gtatgtggct tcactcaatg cttcatgtgc atcattataa gcaattggga
    11461 caccatcatc tacgacattc gcttccacgc cgtataagat tttcacacct gctttttttc
    11521 cagcactatg tgcttccgga aaggcttgcg caccaccatg gtctgtaatg gcgatagctc
    11581 ggtgtcccca ttttcctgct tgtgccacaa gatcagagat gctgtttgtt gcatccatcg
    11641 tactcatatt gctatgaaca tgaagctcta ctcgtttttc cccttcagga gcataatctt
    11701 tgcgaggtgt atgtttgact tctatgatat cttgcgcatt catgaccaaa tcacggacaa
    11761 acgtatcttc ttgaatactt cctcgtacct ttaaccagct accgacactg atcgcatcaa
    11821 atacttgttc atctttttcg ccgttcgaga attttttgac gataaatgat gaagtataat
    11881 ctgtgatttt caaagtcaag atcttgcgtt ttgaacgtag ttctctgact tctttgtcga
    11941 atacgtaccc ctcaagtgtg actcttcgtt cttcttcaac gatactgatc attggtgtcg
    12001 ttggttcatc ggctggaata tttcgtccta gctggatggg tccttcaaga gctggcgatt
    12061 gctcttttct ttcctttttc ttttgttcgt ggacaactag actttcagct gcttgcttca
    12121 taaaggcttc tgcctgttct tgtttgcgtt cttcaaaaag tttcaataca cgttccgctt
    12181 gctgctcatc cacttctggt tcgatacgaa attttgggaa tccgtaactg acgaacaact
    12241 cttctactaa tggcaagtat tgttgcttca aataagaaat agctcctgta ctgtcgattg
    12301 gcaagatgac tttacgatct tttatcacag gtacctgcgt tttcaatact tgctgtgcta
    12361 gtggtgtatc gcattgctga ttttccaaag cttgggccca gtaatcttgc aagagctgtt
    12421 catcgaatgt ctggtcatct gctaagatat tcagtttgac ttgtgcaata tctttaaaag
    12481 cgatagtcag atgctgagtc aatgaccgat atagcataat aggcaaaatt ttggaaaaat
    12541 gtagcgtaaa ttcccataaa cgcgatttac gatggaccac tacactatcc atttcaccag
    12601 cagtaagcag tggatgattt ttttctgctt cctctagttg gatttgttcc aacagttttt
    12661 caaataaatc tcgtttttct gacaaagata tacgcccttt ctgatgagaa acaatgcaat
    12721 tgatcgcatc ttatcctctt ctttttcagt accttttagt atactcgttt tttctgagtt
    12781 ttacattaaa aaattggaac tgcgattcaa atcgcagttc caattttaaa agcgaatcat
    12841 cagattgtca aaaacagctg attcagctat ttttattttc tgtgttttaa cgcttattat
    12901 tcgccttggt tcaagaggat tggtaaagta ttcgccaatt cttctttacg tacttcaacc
    12961 atttcgccag ttttcttgat tttcacttct acgattccat ccactgcttt tttaccgaca
    13021 gtgattctga ttggacagcc aatcaaatca gaatccgcga atttcactcc tgcgcgttca
    13081 tttctgtcat caaccagtac ttcatagcca gcttttgtca tcgcttcttc tagttcattc
    13141 gtcaggcttg tttggtattc gtcttttaga ttcatttgga cgatatgcag atcaaatggc
    13201 gcaattcctt ttggccaatt gattccttgt tcatctgaat gttgttctac gattgctgat
    13261 aataaacgac ttacaccgat accataacag cccataatca caaatttttc tcgtccattt
    13321 tcgtctagta cagttgctcc catagattca ctgtaacgag tgccgatttt gaatatatgt
    13381 ccgatttcga ttcctcgagt aaaggcaaga acaccgttgt tgtctggtga aggatctcct
    13441 tcttgtacaa aacgaagatc ttcataagaa agcacagtga aatcgcgttt tggattcaca
    13501 tttttatagt ggaatcctgt ttcgttcgct ccagtaatgg cattcgctaa atcttggaca
    13561 taacggtctg catatacttt tacgtcatct gaaacaccga ctggtccgac agaaccaaac
    13621 tcagcaccta gatatttttg tgcttcttct tctgttgctt cttctaaaaa gtcagcagat
    13681 aaaaagtttt tcagttttac gtcattgact tcatggtctc cacgtaccaa aacaaggaca
    13741 ggttgttcat ccgcaatgaa caatacagac ttgatgattt tttgaggttc aacagaaaag
    13801 aaatctgcta cttcgtcaat tgattttacg ttaggtgtag ctactttctc caattcaagc
    13861 tgtgtttcat gcgatttttt tgatacatag tagcttgtag ccatctccaa gtttgcagca
    13921 taatcacttt cagtcgaata acagatcgta tcctccccga tttctgagat cgccatgaat
    13981 tcctttgagt ctttcccgcc cattgcgccg ccgtctccga tgatcgagcg aaattctaag
    14041 ccacaacgct tgaatacttc ggtataggct ttttcgtaat ctttatatgt ctcgtccaaa
    14101 ctttcttccg ttgcatggaa agaatatgca tctttcatga taaactctct tccgcgaagc
    14161 aaaccgaagc gaggtcgttt ttcgtcacga tatttaggtt ggatttgata aagatttaaa
    14221 ggtaatttct tatatgaatt gatttcatta cgcatcaaat cagtaaaagt ttcttcgtga
    14281 gttggtccaa ggatcatttt tcgatcattg cggtctttga aacgatacaa gtttggtcca
    14341 tatgtttcgt aacgcccgga ttcttcccaa agttctgctg gcaagatggc aggcatcagc
    14401 atttctaccg ctccgatttt ttcaaattct tcccgcataa tgcgtttcag tttttccaat
    14461 acacgatttg ctaatggcag atatgaataa atacccgctg atacttggcg gatatatcca
    14521 gctcttagca agatttgatg gcttaatact tctgcatcat ttggaacttc gcgtaatgtt
    14581 ggaatcaaca tttttgattg tctcattcta tatactcctt tatttcctca aaatagttat
    14641 cgtttgttat tataccctat ttgtcaactt tttctagaaa aagaatcgtt gaatatcgtt
    14701 ccatgtaact agcaccatca acaacattaa aaatccaaag ccagctaatg tcaatattcc
    14761 ttctttttct tgacttaatg gtttcccgcg aatcccttca aaaatattca agactaattt
    14821 cccgccatct aaggcaggaa tcggaagcag attgacgatt ccgagattca ttgaaagaag
    14881 cgccatcaaa ctaataacag ttgttacacc ttgatttgct gcttctgaag acaattggta
    14941 catcatgaca ggtccgccta atttatctaa actgaaacct gtaaatagtg atcctaaggc
    15001 tttgaaaatt tccaatgaac cgctgaaagc ttgacgcgtc cctccaatga tcttatccat
    15061 aaaacctgta ttcattgggg cttttatacc aagttggcca actttttcgc catttgactc
    15121 tacagatttc ggtgtcacgt cgacagattg cacctgacct tctcgttcga tcttaaagtc
    15181 taatgtttta cctggatttt tcgtaatgac tgttgtcaga tcattccatg aatggatttc
    15241 ttttccgtcc acactgacta cctcatcatt ttcttttaag cctgcttctg ccgcagctcc
    15301 attaggcatg atctctccta cgcgattagt gttcgtcact tgaacaccac cttgcataaa
    15361 tgccagaatg atgaataaca cgattgccaa aataaagtta ttcatgggtc cagcaaagtt
    15421 ggtcagcata cgctgccaca atttagccga ttggaactgt acgtcttttg gcgcaattct
    15481 gatttctgtt ccgtcgtttt caataatcgt cgcatcgtga tcaacagggt aggttactac
    15541 ttctgtttcg tctccattca cataaccagt gatagtcagc tcatcttcta aatcataacg
    15601 gctcaattcc atcgggatag catttgtcaa ttggattttc ttacttaaat ttatcttctc
    15661 tacgatgccg tcagaattta gtaaaagaga aagaggcatt cctggtgcca tttctgtttc
    15721 atcatcccca ttgcctgcca ttcgaacata accgccaata ggtaataggc gaagcgtata
    15781 tgttgtcccg tcttttgctt gatgcccata aattttcgga cccataccga tagcaaattc
    15841 gcggacgagg attcctgatc gtttcgcaaa gaagaaatga ccaaactcat gaacaatcac
    15901 taatatacca aaaacgatga taaatgtcag aatcgttttc atagcttttt tctcctttac
    15961 ttgttttttg tgttttttat aaaacaacga taaaacacta taattttacc atagtatacc
    16021 aaagacacca atagtatcaa tataatgaaa aataaaaacc tgcgtctttt atgaaattca
    16081 caaagaaacg caggtatact tactaaataa tgccaaataa atgcatgata ggaaatacaa
    16141 acagcaaact gtcgaaacgg tctaagattc caccatggcc tggtaagata tttcccgagt
    16201 ctttcacatc ataatgacgt ttgattgcag attcgaccaa atcaccaaat tgtccgactg
    16261 ctgaaaaaat aatcgtcatc agaagcatga caaacatacc atgattgaat agttcttttg
    16321 ctgggtatag caataaataa agaaatgcga ctacaacagc actaccgata ccaccgagcg
    16381 caccttcaat cgttttgttg ggcgaaatat caggacttaa cttatgctga ccataacgtc
    16441 gaccaaccat atatgctccg atatcagttg cccaaacaat aaatagacca aatatcaaaa
    16501 cagccaagct ttctgttcgt gcattgacaa aattctggaa acctattccc acatacaagc
    16561 tgacaagcac aggaaaacct gcttcatcaa tcgtatacgt atttttagaa ataactgatg
    16621 ttcctaaaag aatcataact ataagataaa ataacataaa tgtacttaca ttctctggaa
    16681 gaaagaaaaa ccatctttct tttggcaaaa caagtaacac tgcaccaacg gcggataaaa
    16741 ctccttcaaa acttaagagt gtcaatcctt tcatccggaa cagctcgtaa actcctacta
    16801 ccgctaagat tgctgctgta agctcaaccg ctatgcctcc gatccatata atcggaataa
    16861 acaagactag cgccacagct gctgtgatca cacgttgttt catgactgat cccctttctc
    16921 atcttctgtt ttttttacac cgccgaatcg acggtctcga ttttgataag aggcgattgc
    16981 ttcttcaaga tgcgcaccat caaaatcagg ccaaagcgct tttgtaaaat acagctcact
    17041 atatgcgatc tgccataaca gaaaattact aattcgctct tctccacttg tacggatcat
    17101 cagctcagga tcttgtaact ctttaggcaa gaaacctgtc attaaatgct tagcaatcaa
    17161 ttcatcatcg atttcttctg cgtgtatttc tccttttgct acttcttcgg caatttcttg
    17221 gacagcagtc acgatctctg ctctacttcc ataattcaaa gcaaaattca ataccattcc
    17281 agtattgttt tttgtttgct caattgcacg tcttacagca tcttgcgtat gttctggcaa
    17341 tacgttttca taacccatga catgaacttt gacattttct ttaatcaatt ctggtacgaa
    17401 cgtatcaaag aagtcgacag gtagttgcat gagaaaactt acttcatctt ttggtcgctt
    17461 ccagttttca gtcgaaaagg cataaagcgt caacactttc actccaagcc ttgatgcttt
    17521 ttttgtgact ttctttactg tttccatgcc ttctttatgg ccagcaactc gtggcaaacg
    17581 acggttttgc gcccaacgac cattgccgtc catgatgatg gcaatgtgct gaggaattgg
    17641 tccatcggta ttaaattgaa attcgctttt ttcttgaata tatttgtttt tttgcggaaa
    17701 aaaacgtaac attctttttc ctcctatact taaaagattc ccactatctt aagtattata
    17761 gcaatatcta actgtttttc ctatgaaatc attgtttttt tatttaaaaa ctgaataatt
    17821 ttttcttttt ttaattttta aataaaagaa atataaataa aaacagagga agcggaatga
    17881 tccgcccctc tgtttttatg attttattaa acttccaata attctttttc tttatcagca
    17941 gcgatcttat ctacttcttt aatgctgtta tctgtcaatg tttgtacgtc tttttctaat
    18001 ccgcgcaaat catcttcggt aatatcacca tttttttgtt gttttttata ttcatcgatc
    18061 gcatcacgac ggatgttacg aatcgcgatt tttgcatttt ctgcttcttt tttcacatct
    18121 ttagctaatt cttttctgcg ttcttctgtc agttgcggga taactaagcg aatgacattt
    18181 ccatcattgg ttggactgat tccgatatca cttgccataa tagctttttc aacatcttga
    18241 agaatgctct tgtcaaaagg tgtgatcatt aaaacacgtg cttctggaat ttggattgat
    18301 gccatttggt tcaatggtgt tggcgcaccg tagtagttca cagtaatacg gtccaaaagg
    18361 ctagcgttcg cacggcctgc gcggatttgc ccaagttcac gttgaagatt ttgtgctgct
    18421 tttttcattt tttcttttgc ttctgtcata attgcatctg ccatttttat ttccccctta
    18481 cagttgttcc gatattttcg cctaagattg cacggcgaat atttccgtgt tcatttaaat
    18541 tgaagacaag cagtggaata tcattatcca tgcttagtga acttgcagtt gaatccatga
    18601 cttgtaatcc tttggcaata acttccagat gagtcagttc ttcaaatttt actgcatttg
    18661 catctacttt cggatcagca gaataaacac cgtctacatt gtttttagcc atcaggataa
    18721 catctgcgcc gatttctgcc gctcgtaatg ctgctgttgt gtctgtcgag aagtacgggt
    18781 tgccggtacc gcctgcaaag ataacgatcc gtcctttttc taaatgacgt tctgctttac
    18841 ggcgaatata tggctctgcg atttgacgca tttcaatcga agtttgcacg cgtgtcggta
    18901 cacccacgtt ttccaacgta tcctgtaaag ctaaggcatt cataactgta gctaacatac
    18961 ccatgtaatc agcttgagca cgttccatgc ccatttgtgc tccgatttgt ccgcgccaga
    19021 tatttccgcc acccacaacg attgccattt caacgcccag ctcgtgaact tctttgattt
    19081 cttggatgat ttctttgatt actggtggtt tgattccaaa tcctgcatcc ccagctaaag
    19141 cttctccact caattttaag actacacgct gatatttcgg tgtgaccatg ttcattcctc
    19201 caatattttc tattgccatt ttaccatact tagatgattt cgacactcta agactggttt
    19261 ttcttgtttt gcttaaaaaa agggagcgca caaaccgtgc gttcccgtct gttattcaac
    19321 agaattattt tttcacttgg ctcataactt cttctacaaa gttatcttcg cgtttttcga
    19381 taccttcgcc aacttcgaaa cgtacgaatg ttttaacagt tgcgcctttt gatgcaacgt
    19441 atttttcaac agtcatatca ggatctttaa cgaatggttg gtccaccaaa gcgatttctg
    19501 ctttgaattt cttcaagcgt ccttctacca ttttttcaac gatattcgct ggtttgcctt
    19561 cgttcaatgc ttgttctgta agaacagttt tttcatgttc taattcagct tctggaattt
    19621 gtgattcgtt tacataacga ggattgatag ctgctacgtg catagctacg tctcttgcaa
    19681 ctgtttcgtc agttgttcca tctagaacag tcaatacggc aatacgtccg cccatatgca
    19741 agtatccacc aaatgcagca ttgtcgtctt tttctacaac ttcaaagcga cggaagctga
    19801 ttttttctcc gataacttga gttgcttcga tcaagtcata ttcaattgta cctttatctg
    19861 ttttgatttt cattgcagct tccatgtctg ctggtttgtt ttcagcaaca agttctgcaa
    19921 tttcttttac taaatcttgg aacatttcgt ttttagaaac aaagtctgtt tctgagttta
    19981 cttcaacgat tgctgcagtg ttacctttca cagcaactga agccaatcct tcagctgcaa
    20041 tgcggtcatt tttcttcgca gcctttgcca ttcctttttc acgtaaaaga tcaactgctt
    20101 tttccatgtc gccttctact tcaactaatg cttttttcgc atccatcatt cctacgccag
    20161 tcatttcgcg tagttctttt accattttag ctgtaacgtc tgccatgttt aactcctcct
    20221 gtgtgttttt ctttttaaaa aaagctgctt caaaggagag tgaggcttgt cgcccagcct
    20281 ttgaaacagc tccatcattc aaaattattc tgctgaagaa ttgtcgcctt caacaacatc
    20341 tacgatttct tcgattgatg ttgctgtgtt ttcttctgtg aaatcttctt caactacttg
    20401 atcttcacct tggtttcctt cgatgaaagc atcagccatt tttgaagtaa tcaatttaac
    20461 ggcacgaatt gcatcgtcgt ttgaagggat tactacgtcg atttcatctg gatcgcagtt
    20521 tgtatcaacc atagctacga ttgggatatt taatttgtgt gcttcttgaa cagcaatacg
    20581 ttctttacgt gggtcaacga tgtacattac atctgggatt cttggcatat cagcgatacc
    20641 gcctaagaat ttttcaagac gttcacgttc tttgttcaaa ccagcaactt cttttttagg
    20701 tagaacttca aaagttccat cttcttccat tgcattgatt tgtttcaaac ggctgatacg
    20761 tttttggata gtatcccagt ttgtcaatgt tccgcctaac caacgatggt taacgaagta
    20821 ttgtcctgca cgagttgctt cttctttgat tgcttcttgt gcttgttttt ttgtacctac
    20881 gaataatgct acgccgcctt cttcagcaac gctcttcatg taatcataag ctgcatctac
    20941 taatttaact gttttttgca agtcaatgat gtagattccg tttctttctg tgaagatata
    21001 tttcttcatt tttggattcc agcgacgagt ttggtgacca aagtgtacgc cggcttctag
    21061 taattgtttc atagaaatta ctgccatgtt tgtttcctcc gatttggttt tatttttcct
    21121 cttcttatct tctaccttgc cggcaaactt tttcaagcac ctaccgacaa tccaataaga
    21181 atgtggattt gctgatgcat aaagcaccct cagctattat acagacaaaa gatccgtatc
    21241 gcaagagcaa atatactttt ctgctcaaca ttcaatagtt tacctgaaag ataaaaagaa
    21301 ttcaagaaaa acagtagatt tctctttgtt tctttattat cctttttcca taaaataatt
    21361 ttaggtataa aagacgaata ttattgggca atccttcttt tttttgttat gatacatgtg
    21421 aaatctttcg taaaggagaa agaaaatgcg caaagctcaa agacatttgc tgattaaaca
    21481 agtcattgaa gaattcccga tccgtacgca agaagaacta ttaaacaaac ttgggcatta
    21541 tggcgttacc gccacgcagg caacgatttc tcgagatatc cgtgatctaa aaatcgtgaa
    21601 ggcacctgat gaaaatggcg tttcccgctt cgtattgttt cgcgaaaaag gaactcctga
    21661 gactaggact gaggaagagg atcggctggt tcaaatgatt gaagacattg tattgaaagt
    21721 cgaacgagtc cactttttga cgatcgtcaa taccttgcca gacaatgctc atttatttgc
    21781 atctgtttta gatgagatca aacctccctc tattgtcagt acaattgctg gattcgatac
    21841 gatcatggtc atctcggaaa atgaggagga cgcaaaacga gtagaagaat ttcttcacca
    21901 tccaactgaa gtatcacttt tttaatcaga gcacgtattc ctaatatgga atgcgtgttt
    21961 ttttattttc aatattttcc ttattaaata aacctttaca ctctttacaa aaatacatca
    22021 ttttttattc aattttttca acaccttgat tgaatcacaa taatcatcat ttatattgga
    22081 tgttaacaag agcagcttct tgctctttgt gtatttcaaa aaagaaatga aaggaaatca
    22141 tatgtataaa ttattaactc ctggaccatt gacgacttct gaaacagtga aaaatgctat
    22201 gctgatcgat cattgtactt gggatcaaga atacaaagaa attacacaat ggataagaaa
    22261 aaaattattg gatcttgctc aagtatcgga aggaatatac acaacggtct taatgcaagg
    22321 tagcggctcc tttggggtag aatcggtatt aagttctgtg atcaggccag aagatactgt
    22381 actgatttgt tcaaacggcg catatggaca acgaatgatc aacatgtgcg aatgtctgaa
    22441 actgaactat accgtttatg aggtatcgta acatcaaatc ctccaagctc atgtgattac
    22501 acagctgatt gaagagaatc cctctatctc cgcactagca atggtccata gcgagactac
    22561 ttccggtatt ttgaataatt tggaagagat tgctgctgtc acacaagaaa aagatctttt
    22621 gtttatagtg gacgcgatgt caagctttgg tgggataccg attcgtgtag ccgaactagg
    22681 gattgatttt ttgatttcca gctcaaacaa atgtattcaa ggtgttccag gcttttcttt
    22741 tgtacttgct aataaagcgg aactagaaaa gcgaaaaaat gtgagtcgaa gtttgtcact
    22801 tgatctatat gaccagtacc gcgtgatgga aaacgagaat ggcaaatggc gtttcacttc
    22861 ccctacccat acggttttgg cttttcaaca agcgctgaag gaactagagg cagaaggagg
    22921 aatcgaaact cgatattcac gttatctcgc caataatcat gtactacgac aaaaaatgac
    22981 agagatgggg ttccagcctt tcattcaaga atatcagggg ccattcatca cctcttttct
    23041 ttaccctaag gactggcgat ttgattttaa tcatttttat cagtatttaa agcaaaacgg
    23101 atttgtgatc tatcccggga aaatcagcga tcatgaatgt tttcgtatcg gaaatattgg
    23161 cgagatctat ccggaagata ttttggaact gacagcaatt atcaaagact ataaggagaa
    23221 cgttaatgat taaaactgta atttttgatt gggcaggaac aactgtggac tttggctgta
    23281 tggctcctgt ccacgctttt agaaatgctt ttctagaaaa aggaatccaa ctgactgaca
    23341 aagaaatccg cgaaccgatg ggaaaattaa aatgggatca tatccaacag cttctttccc
    23401 tcccttctgt caaaaaacaa tggatagcta tttatggaca actacctcag gaaaaagagg
    23461 tggaggaatt gtatcaacaa tttgaacatt atctgtttaa agatttggtc aagcattcta
    23521 ccttgaaacc cgacacattg gaaacgatcg aacggttgag aaacgctggg atcaatattg
    23581 gttccacgac tggatttact gctcctatga tgacaatcgt agcagaaacc gcaaaaaaag
    23641 aagggtattc accagacttc atggcaactt ctgaagacgt cgaagggtat ggtcgaccgt
    23701 ttccttatat gatccataaa aacatgcagt acttcaaaaa caagtcggtt gatgaagtaa
    23761 tcaaagtcgg agatacggtc tcagatatcc aggaagggaa aaatgccgga gtgttgacag
    23821 taggtgtaat tgaaggtagt tcgctaatgg ggatttctta tgatgagtac caaaaattag
    23881 agatggggaa aaaagatcgt ttaaaacagt ctgttgctct tagattccaa caagctggcg
    23941 ctgatatttg tgtaaacaat ttaaaagagt taacagaata cattttgtac gctgacaaga
    24001 atccagaatg acttttatac aaaatcagag gtcgtgaaaa agctgtactg catcaagtaa
    24061 taagaacagc caaacaaaaa tagctcctca tatttttcgt ttggttaggc ttattaccgc
    24121 agatgcaagc ttttgaacac cgtttattcg aaaaaaaaag ggagctgtga cacgactttt
    24181 gtcacagtcc ccttatttac aaacaaacag tgtttaagag acaacctctc cactaaacgg
    24241 tgttcattca gctgactctc ggtatttgct caaaaaacga taaaatttga aaagctattt
    24301 tctcttttgt ttctctaata ctcggatcag aacgctgatt tcacattctc tttttcttac
    24361 atcttatctg gtgcatgaag tcctagcaaa cgaaggtctt ctttcaataa tacagtcact
    24421 gcataaacta gagctaagcg tgcttctttt tgttcatcat cagctaaaat cttcgtatga
    24481 gcatagtatt tgttgaatgc ttgtgctaat ttgattgcat gtttcgcaat cactgacggt
    24541 tcgtattttt ccccagcaga caagaccgtt tcagggtatt tttgaactaa tttaaccact
    24601 tcccagctgg tatcgtcatt caatgcatag tctgctgttt ctgaagggac aaatcctgct
    24661 ttttccaaca ggctgactgc acgagcatgt gtatattgca cataaggtcc agtttctcct
    24721 tcaaatcgaa caacttcttc caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa
    24781 tcgtggaaaa tgactgcccc aaccccaact tgtttagcta cagcatcttt gttttccaaa
    24841 tcagggtttt tctcgctgat ttgttcttta gcagattgga ttgcttcatt taatacttct
    24901 tctaataaga cgatctttcc tttacgagtt gataattttt taccaccttg cgtgatcaaa
    24961 ccaaatggaa tatggcgcat atcgtcagac cagtcaaagc ccatttcctt caagacagct
    25021 ttcaactgtt taaaatgata gctttgttca ttccctacta catatagaga ttgtttgaag
    25081 tcgtaagtac gtttacggta caatgcagca gctaagtcac gtgtaatgta tagtgtcgca
    25141 ccgtctgact tcttaatcaa cgctggattc aggtcataag cagataggtc aacgatctct
    25201 gcacctttat cctcgttcag caaatgtttt tcttctaaaa gtttgacgat ttcatccatc
    25261 ttgtcattgt agaaagcttc accgttcaac gaatcgaaac gtacttccag cagatcatag
    25321 attttattga attctttcat cgattcatca cggaaccatt gccataattg aatagcctct
    25381 tcatcgcctt cttccagacg tttgaaccaa gcacgagctt cttcatttaa ttctggttct
    25441 gtttcagcta cttcatggaa ttggacatat aaacgaagca attcattgat tggttctgct
    25501 ttaacagctt cttctgtacc ccattttttg taagcgacga tcagttttcc aaactgtgtt
    25561 ccccagtcac ctaaatggtt gatgcggatt ggctgataac cgattttttc cataatgaaa
    25621 ccgatcgaat taccgataac tgtcgaacgc aaatgtccca ttgagattgg tttggcaata
    25681 ttaggagaag acatgtcgat tggtactgtt ccttgattcc cgatattgct atccccataa
    25741 tgttcttttt ctttcactac tgtttgcaaa acttttttgc tgataagttc tttattcatg
    25801 aaaaagttca agtaaggtcc gacaacttcg attttttcaa agttggcaga atcgattttt
    25861 tctgctaaat ctgcagcgat ttgctgtggc gcttttcggt aaacttttgc taatgaaaag
    25921 gctggaaatg caacatctcc gtgttcagct gattttggat tttctagtaa ttgttcgact
    25981 tgttccagtg ttaaatcatc tttgacagcg tcatagacag cttttgcgac aagatcttta
    26041 ttattcatct ttattcctcc taaagtgctc ccttgattgt taaacaaaaa aaatctctag
    26101 cgacggcata cttagtatgc catcactaga gacgagattt cccgcggtac cactccaatt
    26161 gtcctaaaaa aggaccagct ccatctgata tcggtagacg accgtctgtt taaaacagaa
    26221 ttctccaagt gcgcttcgtt gtatcttctt cattcggctt ccaccacttc cgaattcgct
    26281 agccaagaat gatacaagta ctctcttgtt caacaaatct tagtcttatt aaactttctt
    26341 gaattatatc agaaaaaaaa acgaaaggct agtcgtttct gataaaaaaa caatccagat
    26401 ggcctaaagg agaagaaata ccacctggat agctataaat catttcgtaa atacgaaaga
    26461 cttaacaaat taatcagcta cgataatcgt tccttcttca gaagctaaaa gattctcgat
    26521 attttctaaa gaagtgatga ttgccttagc gtttggtttt gcttcaacaa atgcaatagc
    26581 tgcctctact ttcggaagca tgcttcctgg agcaaattgc ttttcatcaa tgtattgttt
    26641 catttctgaa acagtaacag tttctaattt cttttgatct ggtttttggt aattgacata
    26701 cacattgtct actcctgtca atacgatcag caaatccgca tcgatgattt cggctaattt
    26761 ttctgaagca aaatctttat cgattacggc ttctcgtcct tctaatccag tagctgtttc
    26821 tacaacagga atcccaccgc caccaacaga gactgtgata actccttgat cgaccagcgt
    26881 ttcgatgaca cgtgcttctt taatcgagat gggtttaggg gaagcgacta cttttctcca
    26941 tccacgtcca gcatcttctt tgaatgtcac agtggagtca gcattcattt gttctttggc
    27001 ttcttcttcc gtgtagaatg gaccgactgg tttacttgga tttttgaatg aaggatcatt
    27061 ttcatccaca ataacttgag tgactaaaga aaccacatct ttatcgatcc ctttttcctt
    27121 cagtacttcg cccatggcat tttgtaacca gtaaccgata gatccttctg tcattgcaac
    27181 acatgtatct aaaggcattg ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa
    27241 taggttaccg acttgcggac catttccgtg ggagatgatc aattcatctc cttgttcgat
    27301 aaatttcact aaatactttg ctgtttccat taacgcttct tgttgggctt ttgcacttgc
    27361 atccgtagac aagattgcat taccccctaa agcgacaact acttttcgtt tggccatttc
    27421 attcgtcctt tcttcagttc taccgaattt tctagataac aaaacgagcc agcgttttgt
    27481 gattttccct caatgctgac tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg
    27541 agggataaac aagttaccta atgttgctgc catgatcgct ttgattgagt gcatacgatt
    27601 ttctgcttgg tcaaattgac gagcatattt gctgcggaag acttcatctg ttacttccat
    27661 ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa
    27721 agctggcaag cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt ctaccatttc
    27781 catattgatt tggtaaggtt tcaacaattt gatacgttct tcgaatttat cttcttcacc
    27841 cattgatacc catacatcag aataaaggac gtctgctccg tctacacctt tatcaacatc
    27901 gtcggtaatc atcaattgtg caccggattt ttcagcaaag ccttctgcca ttttgacgat
    27961 ttcttcttca ggttgtaatt cttttggtgc aacgatacgc atattcgctc ctaaaattgc
    28021 acctgtgact aataaagagt tagccatatt gtttcgacca tctccgcagt aagcaacagt
    28081 gataccttct actgttccaa agttttcttg gatagttaaa aagtcagcga tcatttgtgt
    28141 tggatgccat tcatcagtca agccattcca aactggaaca ccagagaatt ctgctagttc
    28201 ctctaccatt ttttgactga atccacggaa ttcgattcca tcaaacatac gacctagtac
    28261 tttagctgta tcttctgtgg attctttttt acctaattgg atatcatttg cacctagata
    28321 ttcaggatgt gcacctagat cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga
    28381 tgttttttca aataaaagcg caatattttt tccttctaga tagtgatgtg ggataccacg
    28441 ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt
    28501 aaagtctttt tctgctaata agcttcttcc ttggaatact gattctttca tattaaacat
    28561 tctcctttag attgttttta tttaagatta aagaggaaat cccgacggca gctgacagta
    28621 agaccgccgt caggaaacaa attacttcaa gtcttcacgg acaagtggtt ggctcatgca
    28681 gcgtggacca ccacggcctc gtgacaattc acttgaattg atttctaaca ctttgatgcc
    28741 atagctacga agcaattcgt tcgatacata atttcggtta taagtgacta caacacctgg
    28801 agcgattgcc aatgtgtttg atccatcatt ccattgttct cgaggtgcaa cgatctcatc
    28861 tccattacca gttgggatca gtacaagatc atctaatcca agagcagctt tcatcgtttc
    28921 gtgtaagtta tctgaatgag taatcttgat atcatcacca tctggttcga ttgtaaagac
    28981 atccatctta ccgtctttac tttgaattgc tggatggata gtgaatttat cgtgatctac
    29041 cattgtaaat acggtatcca aatgcatcat tgcacggttg tttggaattt tgattgctaa
    29101 cactttttca aatcctgaat tttttgcgaa taggtggcgt gctaattttt ctaaagcttt
    29161 cgcattagtt cgttgagaaa tcccaacagc tactacttta tcgcttaaga tcaattcgtc
    29221 tccgccttcg atatgatctg gattttcacg atccaaccat acttcgattc ctttatttgc
    29281 aaatcttgga tgatgtttca aaatatattc tgtaaacatc gattcacgac gacgtgcttc
    29341 aaaagtcatc ttattgactg tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa
    29401 gtacaagttt ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg acagatcgta
    29461 tagactgcta gaacgtactt caatatcttt tgtacgaacc ccagccatga ttttatctac
    29521 catttcttgc gtttccattg atagaaggaa ctcatgaaga ccctctctta cagcattcga
    29581 tgcaatatga gattcatcca acattttatt caagaattgt tctttgacat tgcctgcgtc
    29641 aatcgcttct gccgctaatt tttccaagta aagagtctcg acgccttcat tttgcaatgt
    29701 ctttgcaaag ttgtcatgtt cttcttgagc aatcggaaga tatggaatat catcgaacaa
    29761 cagacgatcc ataatatctg gtgtcaaatt ttccacttct tgtccaggcc ttttcagtaa
    29821 aactgtcttt aatttcccta tttcagagaa aacgtgaata ggtttatcca tgttgtgttg
    29881 cctccttctt tttgattaca tgtataggat atcctccttt tgtaatcact ttcataattt
    29941 tgtctcattc aaaatgagac attcctcaca ttaatattcg ataattaaat aattaaacgt
    30001 ttttctattt ttaagcgtaa tttattcatt ttttattctg tatacacttt caaaaaaata
    30061 tttattcatt gaaaaaaatt ttttgaataa attaaaaaaa taaattatat tagcgttgtt
    30121 agcgtttact atatgtatta ttgttgtttt ttatacacaa atcattcgct tcaaaacaaa
    30181 ttattcaata ccattgtata ttttttacat aataacaaat tatatttgta aagtattaaa
    30241 acttgtttct aatgttggta tgttaaaact ttacaaattc tttaaaaaaa gtgacaataa
    30301 atatagtgaa caataaggag aggacttttt aatgcgtgtt aataaaatcg attttcactt
    30361 ttcaaaactc agatatcaac ctgattttga acatttctcg gatgaagagt tccaacagat
    30421 caaggaacat actgttcttc gttcttataa aaaaggacag atccttttcg acgatggtga
    30481 tcccagacaa cggttgtata tcttgaccaa aggactcgtt cgtttagaac gttatgatga
    30541 aagtgcaact tattactact atgactatgt gaatacaaat aacctttttc ctatgtcagg
    30601 aatgtttgga gaagagacct acacttatac agctcaagcg atgactgata ttgaaacatt
    30661 ctaccttcct gtttcctact atgaaaaatt agcacgaaaa aatcccgaac agttgatcta
    30721 tatgatacaa aaaatttcta aagtagttga aatacatgaa ttgcgtatcc aaatcggatt
    30781 gacctctagc gcctttgacc gggtaaaaca aaatttattc attttgaaag aagaattggg
    30841 cgtaccaaca aaagatggtc atatcaccat tccttatccg atcaccttaa aagaattagc
    30901 agtgaatagc ggcacgacac gtgaaactgc tggacaagta atcaaacaac tacgtgattc
    30961 cggactgtta gattatgaca aaaagcgttt tactttctgt aagacagcta ctactccagc
    31021 tatgagagaa taacgcgaac aaaaataagc agtgaccctg atatttatca gggtcactgc
    31081 ttattttcct ccctaaaact tctgaaagat ccggcagttt tagatcactg ggcaagactc
    31141 aaagattttc tatgtgtgtt tcagttatgt aatattcact gtttgcaaaa aatactgata
    31201 gattaactag aggaagcgtc ttgtcctcat ccgacctaga ttattcgata ttcctcgaca
    31261 ataaacgatt aatcagtgag ggcaatcaga ggaacggata atgatgaata agctgtaaaa
    31321 aatagggctt tgacaaaagc gtgtcacagc ccttatttcg attaaacggt gtgtacctgt
    31381 caactcttcg gtcacaagtc ataatgttca taaacatgag gagctgtttt ttgaaattgt
    31441 tccttgtgaa tcagagttaa acgacaggct tacgaactct tgattaaacg gcgttcaaaa
    31501 gcttgcatac gcggtaataa gaacagccag gtcaaaaaca tgagaagccg tttttcctgg
    31561 gctgttctta ttacttgatg cagggcagat ttttcacaac ctctttattc agataaaacc
    31621 aagcttggat cagcattcaa ctgatagcta gcaaaatttc ttttttgcat ttccacatgt
    31681 gctgctgctc caatcattgc agcattatct ccacaaagtc gaagaggggg aatcactagt
    31741 tctacctcag ggagtttagt tgccaaagca tcctgcaatc cttcacgcaa cccttgattg
    31801 gccgctacac ctccagctac aatcagttgc ttgactggat aattttgaca agctcgaagt
    31861 gttttagtaa tcaatacatc gatcacactt gcttgaaagc tagctgctaa atcatttttg
    31921 tctagatctt ctccacgctg ttgtgcatta tgcaccaaat tgataaatgc actttttaat
    31981 ccgctaaaac tgaaatcata attatcttca tggatcatcg ctcgtggaaa atggtaatta
    32041 tcttttccct gatgagctag ctgatcgatt tcttttccgc ttgggtaaga aagacctaat
    32101 actcttccaa ctttgtcata agcttctcct gctgcgtcat ctcttgtctc cccaatgatt
    32161 tcatatgatc catcttcttg catatagact aattctgtat gtcctccgct aacaagaagt
    32221 gccatcaatg gaaactggaa tggtttaacc agacgggctg catagatatg ccctgccata
    32281 tggtttacag gaataagcgg caattggtgt gcccaagcaa aagccttagc tgcagaaata
    32341 ccaatcagca acgagccgac aagtcctggt ccataagtga cagcgactgc cgataaatct
    32401 tctgccgaca caccagcttc taccagtgca tcttccaagc ataaggtgat ttgttctacg
    32461 tgatggcggc tagctacttc aggaactacg ccaccaaaac gtttatgact gtttatttga
    32521 gaagcaacga tgttagataa gatctctgtt ccatttctaa cgaccgctac actcgtttca
    32581 tcacaacttg attcaatcgc cagaatcaat tcttcattca tttcatcgtc tccgttttta
    32641 actttctgct cataatgatc gcatcttccg tcggattgcg ataatatgat ttccgtttac
    32701 ctaatatacg aaacttttct gattcataaa gttttcttgc tgcttcatta gatagacgta
    32761 cttctaaaaa aacatgcagc gaatctttta tttgttccgt aatcagtaat tgtaacaaat
    32821 gccgtgcatg cccttttcct tgttctgaaa ctgcaacagc aatatttgtg atctcgactt
    32881 catctattac tttcgagtat ccaataaagc cacagatctg gttatcttca actaaaagca
    32941 gataatcaga gtgaggctgt tggatgtctg ccaaaaactg cattttcgtc caaggtgaac
    33001 catgtggata agcattttcg ctaatcatcc atacgcattc tgccaagtac gcttcatcaa
    33061 aatctttttt tctgcaaatc aactaatctc actcattttc taaaagtttt atcatatcca
    33121 acgcatcttc ttttgtttca gtgtaatatc tttttttgat cgttgttgct tgaaaaccta
    33181 acttccgata aagtctttgt gcatcttgat tactcatacg cacttctaat gacatcgtct
    33241 cacagcgatt cattatagca aagttctcga tttcgtcaat aagcagcgaa ccgattcgtt
    33301 tacgctgata tgccggtcga acggcaatat tcgtgatatg gcaatctaaa ccgaacgctc
    33361 ttgaaccgat aaaaccaatc attcttccat tatcaaagat acctagatat agatgaggaa
    33421 gcggtgactt caattctgat ataaaggcac tttttgtcca tggcaattcg ccattataga
    33481 cttctcgttc gacatccagc agctctttga tatcttctgt agtcaattct cttagtacgt
    33541 aacgacgatc gatttgttcg attgtttttt ctgtataaat cattttatct tgaaaaaagc
    33601 ttctggcata cgctctaaat tttttcaaca taactttcgt ctccaggtgt gtgcgtctcc
    33661 aaccattttt cttctgcttc tacccgtttt aaatagcgag gaagaaaagc ttgtttctct
    33721 gcaactggtt cagctaaagc acctaattga gccacagtta caccacttgg aatatcccat
    33781 gctggaactg tattaatcat cgcactaggc aatatttcct ttatttcata gaagaatttg
    33841 tgacaatcac tcccgacgaa ataaatagct tcattcagct tttgtagttg tgtgaacaaa
    33901 tcagaagcag aaatatgtac atcttcaagt acaatttcta atctattttc tttccagcgg
    33961 tatgccccag cgtatacatt ttttcttcga gcatcaaaca tgggcacaat aattttatcc
    34021 acaccgatgc aattagccgc gatggttttc agactagata ctcctactag ctctttatct
    34081 aatgtataag caatcgtttt tgctgtcgtt acacctatac gaagccctgt ataagaaccg
    34141 ggaccatcag aaaccgcgat tcggtcaatg tcttttggac ttatttgaag atctgcaaac
    34201 aattggtcga ttgcaggcat cagtgttaca ctgtggttgc gtttgatatt tgtttgtatt
    34261 tgtccaagaa tttgttcgtc ttctaccaca ccgatcgcca aagtttggtt tgcagtatct
    34321 attcctaatg taatcatata tttgatcctt tctgctatga cattctcgcc ttccattgta
    34381 gcatactttt ttattctgag gatgaaaata ccaaaggcaa aactagtaca atgatacatg
    34441 attatctgct ggcgggcaaa ttttgcccct tcctgaaaca caaaaaagct gcggtatcac
    34501 ttcatttccc cacagctttt ccatcttt
//
LOCUS       DANJMI010000031        34141 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000031 DANJMI010000000
VERSION     DANJMI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.031"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(42..230)
                     /locus_tag="PWU81_001729"
     CDS             complement(42..230)
                     /locus_tag="PWU81_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL1110431.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            500..862
                     /locus_tag="PWU81_001730"
     CDS             500..862
                     /locus_tag="PWU81_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL1110432.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            914..2596
                     /locus_tag="PWU81_001731"
     CDS             914..2596
                     /locus_tag="PWU81_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDL1110433.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2713..4749
                     /gene="recG"
                     /locus_tag="PWU81_001732"
     CDS             2713..4749
                     /gene="recG"
                     /locus_tag="PWU81_001732"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL1110434.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEGSEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4798..5799
                     /gene="plsX"
                     /locus_tag="PWU81_001733"
     CDS             4798..5799
                     /gene="plsX"
                     /locus_tag="PWU81_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL1110435.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5921..6163
                     /gene="acpP"
                     /locus_tag="PWU81_001734"
     CDS             5921..6163
                     /gene="acpP"
                     /locus_tag="PWU81_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL1110436.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6431..7435
                     /locus_tag="PWU81_001735"
     CDS             6431..7435
                     /locus_tag="PWU81_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1110437.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7436..8380
                     /locus_tag="PWU81_001736"
     CDS             7436..8380
                     /locus_tag="PWU81_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1110438.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8380..9342
                     /locus_tag="PWU81_001737"
     CDS             8380..9342
                     /locus_tag="PWU81_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1110439.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9369..10283
                     /locus_tag="PWU81_001738"
     CDS             9369..10283
                     /locus_tag="PWU81_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1110440.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10309..12090
                     /locus_tag="PWU81_001739"
     CDS             10309..12090
                     /locus_tag="PWU81_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1110441.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12438..13124
                     /locus_tag="PWU81_001740"
     CDS             12438..13124
                     /locus_tag="PWU81_001740"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL1110442.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13144..16725
                     /gene="smc"
                     /locus_tag="PWU81_001741"
     CDS             13144..16725
                     /gene="smc"
                     /locus_tag="PWU81_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDL1110443.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16734..17543
                     /locus_tag="PWU81_001742"
     CDS             16734..17543
                     /locus_tag="PWU81_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1110444.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17554..18552
                     /gene="ftsY"
                     /locus_tag="PWU81_001743"
     CDS             17554..18552
                     /gene="ftsY"
                     /locus_tag="PWU81_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL1110445.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18739..21006
                     /gene="gshAB"
                     /locus_tag="PWU81_001744"
     CDS             18739..21006
                     /gene="gshAB"
                     /locus_tag="PWU81_001744"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL1110446.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21095..21547)
                     /locus_tag="PWU81_001745"
     CDS             complement(21095..21547)
                     /locus_tag="PWU81_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HDL1110447.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21697..22647
                     /locus_tag="PWU81_001746"
     CDS             21697..22647
                     /locus_tag="PWU81_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL1110448.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22640..23599
                     /locus_tag="PWU81_001747"
     CDS             22640..23599
                     /locus_tag="PWU81_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL1110449.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23596..24351
                     /locus_tag="PWU81_001748"
     CDS             23596..24351
                     /locus_tag="PWU81_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1110450.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24374..25327
                     /locus_tag="PWU81_001749"
     CDS             24374..25327
                     /locus_tag="PWU81_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1110451.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25971..26267)
                     /locus_tag="PWU81_001750"
     CDS             complement(25971..26267)
                     /locus_tag="PWU81_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HDL1110452.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26622..26978
                     /locus_tag="PWU81_001751"
     CDS             26622..26978
                     /locus_tag="PWU81_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL1110453.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26988..27887
                     /locus_tag="PWU81_001752"
     CDS             26988..27887
                     /locus_tag="PWU81_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDL1110454.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28404..29135
                     /locus_tag="PWU81_001753"
     CDS             28404..29135
                     /locus_tag="PWU81_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL1110455.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29280..31307
                     /locus_tag="PWU81_001754"
     CDS             29280..31307
                     /locus_tag="PWU81_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL1110456.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            31415..32512
                     /locus_tag="PWU81_001755"
     CDS             31415..32512
                     /locus_tag="PWU81_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL1110457.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            32819..33277
                     /locus_tag="PWU81_001756"
     CDS             32819..33277
                     /locus_tag="PWU81_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL1110458.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKLTRV"
     gene            33375..>34141
                     /locus_tag="PWU81_001757"
     CDS             33375..>34141
                     /locus_tag="PWU81_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HDL1110459.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSR"
BASE COUNT        11462 a         5920 c         7274 g         9485 t
ORIGIN      
        1 aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt
       61 cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg
      121 cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg
      181 ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc
      241 tcctttgcct tgatttggag caatagaata attcagctca taccagacta acatatcata
      301 tagaatggaa atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt
      361 gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg
      421 ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac
      481 ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat
      541 taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt
      601 aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa
      661 ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac
      721 gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa
      781 atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg
      841 tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag
      901 gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca
      961 ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc
     1021 tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc
     1081 agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt
     1141 gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc
     1201 taagcaaatt ccagatgttg tctcattaaa tgcttccgat cttgctgcag ccttcactca
     1261 tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt
     1321 agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga
     1381 agtaatgaca gctgttgtaa aaggggcaaa gcgtgcttta gctaaaactc cagacttgct
     1441 tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta
     1501 tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc
     1561 tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat
     1621 ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg
     1681 acctacagtc gatagtacat tcgattacga agaattccgt aactatttag atggtatagg
     1741 agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa
     1801 cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga
     1861 caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc
     1921 gaaaaaagaa acaccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt
     1981 ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtgggc aaacaatgaa
     2041 tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt
     2101 cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat
     2161 cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt
     2221 caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt
     2281 tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa
     2341 agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac
     2401 agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat
     2461 cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga
     2521 aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct
     2581 attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa
     2641 aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaagggagg
     2701 gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa
     2761 aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat
     2821 tatcctttcc gctacgaaga tattcaggaa agaaatctaa acgagatcca agatcaagaa
     2881 aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa
     2941 aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt
     3001 aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa
     3061 tgggatgcaa agcgtaaagc attgaacggg atgaagattt taggttctca aacaatggat
     3121 gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta
     3181 atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt
     3241 gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa
     3301 gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt
     3361 caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc
     3421 ctttatgatg tccaaaaatt gaaagaattt actcaaaagc taccttttga actgacaaat
     3481 gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag
     3541 cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac
     3601 gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa
     3661 caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgctt
     3721 acggggtcaa cgaaagcaaa agaacgtcgt gagctccttg aaaaactaga gcaaggggaa
     3781 attaacatcg tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta
     3841 ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt
     3901 gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta
     3961 gctattacag catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt
     4021 atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg
     4081 aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaaggatca
     4141 gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca
     4201 ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc
     4261 atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta
     4321 ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg
     4381 gcacagctgc atcaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc
     4441 ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc
     4501 accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt
     4561 ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat
     4621 gttttggagg tagccagaga agaggctgca gccctttggc agataaagga ttggcagatg
     4681 cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat
     4741 ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg
     4801 aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg
     4861 gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa
     4921 atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc
     4981 aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt
     5041 gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc
     5101 gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc
     5161 ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt
     5221 ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt
     5281 tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca
     5341 gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca
     5401 agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta
     5461 gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg
     5521 aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc
     5581 gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat
     5641 ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc
     5701 cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt
     5761 ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcaat
     5821 ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg
     5881 taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt
     5941 taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga
     6001 tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt
     6061 agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac
     6121 agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa
     6181 gaaggataaa aagctgcata cagcttttta tccttctttt tttaatcttt ttgacacgta
     6241 aaagtcattt ttctgataat tctggcgata aacagaaatt atgttgcaaa gtaaagcgat
     6301 tttctatata attattaata attcattaca caaagataag aataaccttt gttataaaac
     6361 gctttattct gtgtaatgtc cttttttatt aagaaaaact tccatttagg aagcagagag
     6421 gggtattcga ttgtccgata accaattatt agaagttcag aacctacata caggattccg
     6481 actcaaagat gactattacg atgcagtaga tgacgtttca tttactttag acaaaaacga
     6541 gattttagca atcgttgggg aatctgggtg cgggaaaagt accttggcta ctacgattat
     6601 gggattactt gatcctaata acacaaaaat tacaggtgag attttatata acgatctgaa
     6661 tttaatcgac ctgaacgaaa cgctctataa taaaatccgc ggaaatgata tcggaatgat
     6721 tttccaagat ccgttgtctg cattaaatcc tctcatgaga atcgaagacc agatcaaaga
     6781 aggactttcc tatcacacaa agatgaatgc agaacagcgt caagctcgtg cactagaatt
     6841 gctagaacaa gtagggattc ctaatcctgc gagagttgga cgccaatatc cccatgaact
     6901 atcaggcggt atgagacaac gtgtcatcat cgcaatcgca atcgcctgca aacctccaat
     6961 cgtcattgca gatgaaccga cgacagccct agatgtaacc atccaagcac agattcttga
     7021 tttactgaaa gaccttcagg aagaaacaaa aacagggatc atcttgatca ctcatgactt
     7081 aggagtcgta gcagaaatgg ctgatcgagt ggcagtgatg tatggcggac agttcgttga
     7141 agtagcaggt gtaaaagaac tgtttgatca tcctaagcat ccttatacac gctctttact
     7201 gaactctata ccacaagaag gaaatcatga tgcagaactc catgtgatcg aaggtgtcgt
     7261 tccttctctg aaaaacatgc ctcgtaaagg ttgtcggttt gcaccacgta ttccatggat
     7321 accagcaagc gatcatgaag aagagcctat ccttcatgaa gtcgcaccga accacttggt
     7381 acgttgctcc tgctataagc atttccactt tagagacgaa aaaggagagg tgtagatgac
     7441 agaattaatc acaatcaaag acctgaaagt tcattaccct attcgcagtg gattcttcaa
     7501 tcgagtaacg gatcatgtat atgctgtaga tggtgtggat tttattatcg aaaaaggaaa
     7561 gacatatggt ttagtgggag agtctggctc tggaaaatct actacgggaa aagccgttgt
     7621 gggattagaa aaggtaacag ccggacaaat catgtatgaa ggaaaagatg taacgaagcg
     7681 aagcaaccgc aaaaaaatgg gttataacaa ggatgtgcag atgatcttcc aagattcaat
     7741 gtccagtttg aatcctaaaa agcgagtgtt ggatatcatt gctgaaccca tccgaaattt
     7801 tgaacggtta agcgatcaag aagaaaagaa aaaggtaaaa ggactgctag atatcgttgg
     7861 gatgccggaa gatgcgctat ataaatatcc tcatgaattt tcaggcggac aacgtcaacg
     7921 tctaggagta gcccgtgctg ttgctaccaa tccaaaactg atcgtagccg atgaacctgt
     7981 atctgctctg gatctatctg ttcaagcgca agtattgaat ttcatgaaac gaatccagca
     8041 agaatacggc ctcagctatt tattcatttc acatgattta ggtgtagtaa aacacatgtg
     8101 cgacaatatc gcaattatgt acaaaggtcg atttgtagaa atcggtacaa gagaagacat
     8161 ttataatgac ccgcgacata tctatacgaa acgtctgctt tcagctattc cgcaaatcga
     8221 tgtagaaaat agggaaagcc acaaagaaaa tcgacgaaga gtagaacaag agtatatcca
     8281 aaaccaaaaa gaatactatg atgcgactgg tcgagtctac gatttgcgta cgatcacaac
     8341 tacacacaaa gtggcgttga aagatggagg tgcaagctaa tgtggaaaac gattctgcga
     8401 cgtgttttat tgatgatccc gcaagttatt attttaagtg ttctgatctt cttattagcc
     8461 caagcgatgc cgggggatcc atttaccggt ttgatcaatc caaatcagga tccggcagtg
     8521 atcgaacaga tgcgacaagc agctggcttg aatgacccat ggtatgtcca gtacttccgt
     8581 tggatcggaa atgctttgca aggcgatttt ggtcaaagtt tcttatacaa acgctctgtt
     8641 gctgacttga tcggcgaacg gatcatcaac actttatatc tatcgatctt gacagttgtc
     8701 cttacttatt tgattgctgt gcctctgggg atgcttgctg gccggtatca aaattcaatc
     8761 ttggataaga ttgtcgtaat ctacaacttc tttagttttg ctgttccttt atttatcttc
     8821 ggcttgatca tgctatttgt ttttggttat cgtttaggct ggttcccaac aagtggatca
     8881 caaacactag gtttgcaagg tggatttatc actcaatgga tagaccgttt gaaatatatt
     8941 ctgttaccat caattaccca agcatttttg gcgactgccg tcacaatcca atatctgcga
     9001 agtgaagtaa tcgattcgaa atcgctcgat tttgtcagaa cagcccgttc caaaggggta
     9061 ccgacgaata aagtattcag tcgacatatt ttccgaaatg cagcgttgcc gatggcttct
     9121 caaatggggt atgaaatcac ttcgttgatt gctggttctg tagttatcga aaagattttt
     9181 ggttatccgg gagtcggaaa actatttatt gattccatcg gccaacggga ctataccgtg
     9241 atcacatcct tagtattgat tttaggaatc gcgacgctag tggggacatt actttcagat
     9301 atcatcatga gtatcgttga cccgcgtatt cggattcaat aaaaccaatc agaaaggaga
     9361 atggatcgat gagtgaaaaa aacaatacag cagttcaaga aagtattcct cctatgggga
     9421 tacgaatgat tgctcgagaa tttaaaaaag ataaaattgc tattttttca ttaggtttgc
     9481 tagtggtttt attattagtg atctttatcg gtgcgatgct gaccgatcaa gacaaagtga
     9541 tgtatgttag tatcttcgac aaatacgctg caccaggaac agtttcgaca caaggacaaa
     9601 agttcattct gggagcagat gaaggcggac gtgatgtatt aggacaattg atcataggtg
     9661 ctagaaactc tgttacgatc ggatttgcga tcacgatcct tacttcgatc atcggggttg
     9721 gattagggat catctctggc tactacggag ggatcattga caacatcttg atgcggatcg
     9781 ttgattttat catgattcta ccgatcatgc tgatcattat cgtcttcgta tcgattattt
     9841 ccagatacaa tgtctggtcc tttatattca tcatgagtgc attttattgg gtagctaaag
     9901 cccgattatt ccgaagcaag acattatctg aagcaagaag agattatgtc agtgcctcaa
     9961 aaacaatggg aaccaatgat tttaaaatca tgttccgcga aatcatgccg aatctaagtt
    10021 cattaatcat tactaatttg acgatgaact ttgcggcaaa catcggtatc gagacaactt
    10081 tgactttctt aggtttcggc ttgccggcaa atgtacctag tttaggtaca ttgatcggtt
    10141 atgcaagtaa cggagaagtc ttagtgaatc ggcaatggat ctggttaccc gcatcaatcc
    10201 ttattttagt tttgatgttg agtatcaatt atgttggaca agcattcaag cgttctgcag
    10261 atgcgcgcca acggttaggc tagtatagaa gcgaaggggg aaataggaat gaagaaaacg
    10321 atttttggat ttgcagcgct gatgtctgct gtcgttcttg cagcatgcgg gggcggaggt
    10381 acagatacct ctggaaacaa ctctggaggc aacggcaata acaaagagga attaactttt
    10441 ccaattgcaa caaaaaataa tgatgatgca atcgaagacg gcgaattaga agctgcggtc
    10501 gcaacggaca gtcagtttaa aggattgttc cagtgggagt tttaccaaga ttcatatgat
    10561 gcagctttta tggctccttc tcatgaaccg ctatttgcaa acgatgacga tttcagaatc
    10621 acagacgacg gagctgcaac gcttgatcta gatgaagata cgaaaaaagc gacaatcaaa
    10681 gtaaaagaca acgtcaaatg gtcagatggt gaagatgtaa cggcggacga tgtgattttc
    10741 ccttacgaaa tcattgggaa taaagattac actggtatcc gttatgacga cactttccgt
    10801 aacatcgtag gtatggaaga atatcatgat ggaagtgccg acacaatctc cggaatcaaa
    10861 aaaatagacg acaaaacagt tgaaatcgaa tataaagaag taaacccaag catgcttcaa
    10921 tcgggtggtg gtatctggtc atatgcagca ccaaaacata cgttagaagg tgtagcaatc
    10981 aaagatatgg aatcaagcga tcaagtacgt aaaaatccag tgacttttgg cccttattac
    11041 atgagtaata tcgttgcagg agaatcagta gaatacttgc caaacgagca ctactggaat
    11101 ggtacgccta aattgaaaaa gatcacgatg aaatctgtac cgatggcaag tgcaactgaa
    11161 gcattgaact ctaaattata tgatatgatt ttccaaatgc caacagatac ttacgatacc
    11221 tataaagaaa ttgctggcta tacaaactta ggccgtgaac aaacttctta tacttatcta
    11281 ggattcaagt taggtaaatg gaatgcagat acaaagagtg tggaatacaa tccagattca
    11341 aaaatggctg ataaatcatt acgtcaagca atgggctatg cattagataa taatgcagta
    11401 ggtgaacgtt tctatgcagg tttgagaagc aatgcgactt cattgattcc tccagtattc
    11461 cagtcatacc atgatgatga gatgaaagga ttcacacaag atctagacaa agcgaaaaaa
    11521 ctattagatg atgctggcta caaagatgtg gatgacgatg gtttaagaga agacaaagat
    11581 ggaaataaac taacaatcaa ctttgcttcg atgtccggtg gggaaacagc gcaaccattg
    11641 gcagattact atatccaaca atggaaagaa attggtttgg atgtccaata cacaacaggt
    11701 cgtctgatcg aattcaattc tttctatgat aaattagaaa acgatgatcc tgaaattgat
    11761 gtttaccaag ctgcttggaa tacaggaact gatccaaatc caagtggact atggggacca
    11821 aatgctgcgt tcaactacac tcgttatgaa tcagaagaaa atacaaaatt gatcaacgat
    11881 atcagttcaa gcaaagcatt tgatgaagag taccaaaaag aagcatatca aaaatggcaa
    11941 caatatgcat ttgaagaagc atttgcgatt ccaacgttgt tccgtaatga agtattgcca
    12001 gtcaacgacc gtgtcaaaga ctgggattgg tcttatgatg taccaaaccc atgggcaaat
    12061 gtttctgtta cagcagattc acgttcataa tcactaaaaa tgaccgaaaa agggcgatgt
    12121 cctttttcgg ttttttgata tttttctgtt tatttctcta atattcaggt caaggcgctg
    12181 atttcatgac ttctccaata aacggtgttc aaagtccagt ttctcggtat taagtcaaat
    12241 ttatgcaaaa atatggaaag ccattttcac aaaatttttt cttcatactc gaaactaaac
    12301 gaatttttca caacttctcg tcaatcacta ggattattaa agctttactt accttttgac
    12361 cgaaccattt caaatttgac aaaaaaccag tataataagg aagtatcgaa aaaatatcca
    12421 taaaaggaat gtggaaaatg gacaaccagc tgacaaaaga actaaaagaa aactataaca
    12481 tcgtttttca cgatctaaat ttgcttgaac aagcttttac ccattcatcc tatgtgaatg
    12541 agcatcgcaa tctgcaatta tcggataatg agcgacttga atttttagga gatgccgttt
    12601 tagaactgat ggtttctcag tatttattcc gtttatatcc tcatttgcca gaaggaaaat
    12661 tgacaaaaac acgagctgcg atcgttcgcg aagacagctt gtcgaaattt gccaaagaat
    12721 gtcactttga tcaatatgtt ttgttgggca agggagaaga gaattcagga ggacgtacgc
    12781 gaccagcatt gctttgcgac ctattcgaag cttttttagg cgcgttatat cttgaccagg
    12841 gattcgacac gacccatgca ttcatcgaaa aagtgatttt tcctaaagtc aaagcaggtg
    12901 ctttttcaca tgagatggat cataaaacaa aactacaaga agtcttgcaa aaatcaggtg
    12961 atgtatcaat cgaataccga ttgatcaatg aagaaggtcc agctcatgaa cgagtatttt
    13021 ggatcgaagt ttatgtagat gatcaattga ttggcactgg acaagggaaa tcgaaaaaat
    13081 tagcagaaca agctgcggcg gaaaatgcat tggcagcact gtaaaagtaa gtgagggaaa
    13141 gctttgtatt tgaaacgaat tgaaatagcc ggttttaaat catttgcaga caagacagtc
    13201 attgattttg aaaatagcgt cacagcagtg gttggaccaa atggcagcgg aaaaagcaat
    13261 attacagagg ctatccgctg ggtattagga gaacaatctg ctaaaagtct gcgaggcgga
    13321 aaaatgccgg atatcatctt tgccggttcc gatacccgta agcagctgaa tattgcagaa
    13381 gtcacagtga ttttagacaa tagtgatcat tatctgccat tggagtacaa tgagataagt
    13441 gtaacccgcc gttatcgacg gactggggaa agcgagtttt tcatcaataa acaaagctgc
    13501 agattaaaag atatccaaga attgttctta gattctggat taggaaaaga atcgttctca
    13561 atcatttctc aaggaaaagt agaagcgatt ttcagtagta aaccggaaga ccgtcgtggg
    13621 atctttgaag aagcagctgg tgtcttgaaa tacaagcagc ggaaaaagaa agcagaacag
    13681 aagctttttg aaacagaaga caacctcagc cgcgtccaag acatcatcca cgaattggaa
    13741 gaacagttaa ctcctttggc cgcccagagt gaggcagcaa aggagttttt acggctaaaa
    13801 gaaacactga ctcaaaccga tgtctctttg atggttgctg aaatcaagac agcaaaaaaa
    13861 gattgggaca acaaacaagc acagcttgcg aaattcaatc tagaattagg caaactatct
    13921 gagtcgatcc aagaacaaga atccattttg gcgaagcagc gtaaagaaaa tgcgcaagcc
    13981 gatcgtctga tcgaaaaaaa tcagcaagta ttattagatc tttcggaaaa actgaagcag
    14041 acagaaggac aaaaagatgt tttgcaggag cggacaaagc atacacaaaa aagctcgcaa
    14101 gaatatcaaa cttcattagc agaagcacaa aaaaaggtta aacattttga aaagcttcaa
    14161 gaaagcttga tgaaagcagc agcagaaaaa gaaacagaga tccaaaaagc tgaagcaaat
    14221 ctcataaaaa cacagcaaga attagaaaaa taccaaaaat caacgaaaga attattggca
    14281 gaattaagag atcaatatgt tgatttgatg caagaacaag cagctgtagg aaacgaactg
    14341 aagtatctag aacgtcaata tatacaagaa acagcaaaaa gcaagcagac gttagccaag
    14401 caatcggaag tagaagcatc agtagaccga ttgatcttac aaaaagaaga attgacacaa
    14461 aagcaggcgc agctaaaatc atctttaact gaaacaaaag agaagttaga aatgatccaa
    14521 caaaacggga aaaagttcca agaagcatta gcaaaagaac agcctaaaat gtatcaatta
    14581 atgaatcaag tccagcagct acgtgctcgt aagaaaagcc ttcaagaaat ccaagaaaac
    14641 tattttggtt tttaccaagg ggttcgattg gtcttgcagc ataaacagca gctttcgggt
    14701 attgtcggag cagtggctga actgatcgat gttcctgcag atttcacgtt agctatcgaa
    14761 gcagctttag gtggggcagc ccagcatgtc atcgttgaaa atgaaaaaga tgctcggcaa
    14821 gccatcacgt atttgaaaca gcaaagagga ggaagagcaa cattccttcc actgacgaca
    14881 atcaaaccaa gacaattacc ggctcatata ttgacacaag cagcagctgt agaagggttt
    14941 atagggattg ccagtgaaca agtcagttat cccgatcaaa tccaaactgt ggttcaaaac
    15001 ctgctaggaa cgatcctttt agccaaagac ttaactagtg ccaatgcaat cgcgcaaacg
    15061 atccgctatc aatatcgagt ggtttcctta gaaggtgatg tgatgaatgc cggaggatcc
    15121 atgacaggtg gagcgaataa acgaggaaac caaggcagtt tgtttgtaca aaatcaagaa
    15181 ttgaaacagt tgactagtga atttgaagaa gcagataaac aactccaagc acaagaaaaa
    15241 aaagttcagg agctgcaaca agaaacagct cgtttagccg aagaacaaga agttttgcgt
    15301 acacgaggcg aacaattacg ttttgaagaa caagaagcga caaatcagct acaaaacatc
    15361 ataaatgaac ttgaacgttt tgaaaaagaa aagcaaatct caacttttga gacacgtgaa
    15421 ctgcagcagt ttatcgaaga ttacgaaaag caaacgaacg aattgaaaga caaacaaaca
    15481 gatcttgaat cgcaacgaca aaaaatcgac gaagaaatca aatcgttaag tcaagaaagc
    15541 gaccagatgg aagccagaag agcacaagta caaagtcaaa aagcacaaga acaagcagat
    15601 cttgctgtct tgaaagagca gtttaatcat ctgcagatcc agctccgcgg tgcccgagtc
    15661 caaaaagcag aggcaaccga acgtcaagaa gcgatagaaa agcagttggc gacattgaca
    15721 gcagactttt ctgatcatga agtcacagaa gaaagtttgg aaaaacaaat taacgaatta
    15781 tctgctcagc gagaaacatt aaaagccgag ttggcaaaag ccaaagagca gcgagataga
    15841 aggcaaaagg agatcgataa gcttgaagca gtcttggcag agcgtaatcg tgagcaaaaa
    15901 gcacgactgt cggaacaatc caaattagaa gtccaaaaag accgagctga aatgatttta
    15961 gataatcatt tgacctatct gcagtcagaa tatcagctga catttgagaa agcttcccaa
    16021 gactatcaag aaacaaccga tatcgaagac agtagaacaa aggtttcatc cttaaaagaa
    16081 cagattgaaa aactgggacc agtaaactta aattcaatcg aacaatacga acaagtcagt
    16141 gaaagacata cattcttagc aacacagcga gatgatctgt tagcagcgaa aaaccaattg
    16201 tttgaaacga tggatgagat ggatgatgaa gtgcggacaa gattcaaaga agtatttgaa
    16261 gctatccgtc aagaattcaa aattgtcttt ccaaatatgt ttggcggcgg taaagctgaa
    16321 ttagtactga cggaaccaac tgatttgctg aaaacaggta ttgagattga agtacaacct
    16381 cctggcaaaa aactgcaaag tctaagtttg ctttctggag gagaacgagc acttacagcc
    16441 atcgcgttgc tcttttccat tatccgtgtc tgtcctgttc cgttttgtat cttagatgag
    16501 gtagaagcag cgttagatga agccaacgtg aaacgttttg gtcgttactt gagcgatttc
    16561 caagatgaca cacaatttat cgtagtcaca caccgcaaag gaacgatggt cgcagcagat
    16621 gtactgtacg gagtgacgat gcaggaatcc ggtgtatcaa aaatcgtttc tgtacggatg
    16681 gaagatatca atgaagaggc gaaaatcaac gaaacgaaag tgtaggaacg aaaatgatca
    16741 aattagtagc aatcgatttg gacggtacat tattaaatga acaaaagctg atttcagatg
    16801 agaataaaca agcattagca caagcaaaac agcaaggggt caagatcgtt ctatgtacag
    16861 gacgcccctt agctgcgatg gcgcattatc tgcaagaatt aggactggta gatgaaggtg
    16921 actacagcat cactttcaac ggcggtttgg tacaaaaaaa tgatactggt gaaatcatgg
    16981 aaaagaaagt gatggaagta tcagatatcc aacgcttata caaactggct cagcaattgg
    17041 atctgccact agatgttttg tctgacaatg tggttttaca gctgcctagt gccccaagga
    17101 agcaatcctt gtacaatgtg ttgaataagc tgctgcaatt ccaaccagcg gatataagcg
    17161 atattacaga tgaatttgtg cttaataagg cagtaatcgc ttatgaccaa gaagaattag
    17221 atcctaaaat caaagaaata ccagcagaat tccatgaaat gtatgagatc atcaaaacaa
    17281 gaagtatgct tttggaattt atgccaaaag gtgttacaaa agcttatggt atttctttat
    17341 tggcaaaaga tcttggtttg aaacaagaag aaatcatggc gatcggtgat gaggaaaatg
    17401 atttaccgat gatccaatat gctggactag gcgtagcgat ggagaatgct gtgcctttcg
    17461 tcaaagaagc agcagatttc gtgacagcca gcaatgaaga aaatggcgta gccaaagtga
    17521 tccaaaaatt tgtattagag tagggggagt caaatgggat tttttgataa aatcaagcgt
    17581 gcttttactg gcgaagagaa aacagaagaa gttgaagtac aggagaagta cgacaagggt
    17641 ttagaaaaga cgcgtaaaac atttggtgaa cggatgaatg agttgtttgc caattttcga
    17701 acagtcgatg aagacttttt tgaagaatta gaagaaacac ttatcggtgc agatgtaggt
    17761 tttgaaacag cgatacgaat caccgaagca ttacgtcaag aagtgaaact cagaaatgcg
    17821 aaaaaatcgg cagaagtcca aaatgcgatc atcgagaaac ttgtggatct ctatgaagcg
    17881 gaaggaatca atgaagtaaa tgaactaaat atccaagaag acggactaac agttattttg
    17941 tttgtcggtg ttaatggtgt agggaaaaca acaagtattg gtaaacttgc ccatcagttc
    18001 aaaaacgaag gtaaaaaagt attgatggca gcagcagata ccttccgcgc tggagcaatc
    18061 gaccagttag ttgtctgggg cgaacgcgct ggtgttgaag ttgttcgtgg caatgcagga
    18121 ggcgatccag cagctgtcgt attcgatgct ttggaaagag caaagacaga gcaggcagat
    18181 gttttactag ttgatactgc aggccgactg caaaataaag tgaacttgat gaacgaatta
    18241 gaaaaaataa aacgagtaat ccagcgggaa ttaccagaag caccccaaga aacacttctt
    18301 gtattagatg caaccactgg tcaaaacgcg atggttcaag caaaacaatt caaagaaaca
    18361 acagatgtga ccggtcttgt tttgacaaaa ttagatggta cagcaaaggg cggtatcgta
    18421 cttgctatcc gtaatgaatt gcatttacca gtgaaattgg tcggtcttgg tgaaggaatc
    18481 gatgacttgg agccgtttga tccaaatgat tttgtcgttg gattgttcaa aggattacta
    18541 aaagaagaat aacccatgtc taaaaagcag gagctgtgac aaaagttgca gctccttgtt
    18601 tgaatgattg tttttaacat agctgtgttt ttgatgcctt catttttata cttatttgta
    18661 agcggttctc aaacaatttc gttctttttc ttagactttt tgattataat tagattcata
    18721 tgaattggag gtcacttaat gatgaatttt aagcaattat tattgcatgt caatgcgcgc
    18781 ccttttatcg accaagctcg atttggtata gaacgggaag gacagcgggt tgatcttgca
    18841 ggaaatctag caaaaaccga tcatccagca atctttggcg atcgatccta tcacccctat
    18901 atccaaacag attttagcga aacacaaaca gagatgatca cccctgttac cgattctatt
    18961 cccgaattat ttcagtatct cgctgctgtt tatgatgtga ctgctcgttc tataccgaaa
    19021 gaagagatga tctggccatt aagtatgcca cctgccttac cggaaaaaga cgaagagatc
    19081 attattgcaa aattaaaaaa tttcgaagat gtcttgtatc gacgttattt agcaaaaaaa
    19141 tacgggaagc gaaaacagat ggtaagcggt attcatttca attttgaatt tggcgatgag
    19201 ttgctaagaa cattgttcag ccatcaggaa gaatttcaag atttttccga attcaaaaca
    19261 gaactttatt tgaaaacagc gagaaacttt atgcgctatc gctggatgat cacttattta
    19321 ttcggtgctt caccaatgag tgagaagaac tactttttag atgaatcaca tccgcaagaa
    19381 cctgttcgca gtattcgcaa cagtgcgctg ggctatacga atcatccaaa tgtgaaagtt
    19441 tcctatgctt ctatgaaaca gtatttagca gatatcgagc gaatgatcga agaagggaaa
    19501 ctttcggagg agaaagaatt ttatacaccg cttcgtttcc gaggcggaaa gaaagtcgca
    19561 gatttagcaa caacaggtgt tcgttatatt gaattgcgga atatcgattt gaatccttat
    19621 gcaagattgg gtatcaatcc agagcaagtt cggttcttac aattattcct gatgtacatg
    19681 ttatggacag aagaaaaaga agactgtgac caatgggtag cagaaggtac gactcgaaat
    19741 aacaaagtag cactagaaca accatcagat caaacagaat ttcatcaaga aggtagagaa
    19801 attcttgaag gaatgaagca aatgctggtt gaattggatt ggttggattc tctttattta
    19861 gtagaggaag cattgactca aatggatcat cctgaacaaa cgttagcagc gaaactctat
    19921 caagaagcac agctatcgag tcagcaagaa gttgctgtgg cattgggaca tcaatattat
    19981 aaagaaagcc atgaacgtcc ctatcagttg gccggttttc gcgaaatgga actttctact
    20041 cagatattta tgttcgatgc catccaaaaa ggtgtccaag tcaaagtatt agatgagtcg
    20101 gatcaatttt tgcgtctgca attccaagac catgtagaat atgtgaaaaa tgcaaacatg
    20161 acaagtaagg acagctatat cgtcccactt atcatggaaa acaaaacagt aacgaaaaaa
    20221 gtcttaaaag aagctgggtt ccgggtacca ggaggcgcag aattttcatc catggaagaa
    20281 gcggtaaaag cttatccgag atttgccgag caggcatttg tgatcaaacc taaatcaacc
    20341 aattacgggt taggtatcac gattttcaag gaaggagcta gtctggaaga ttatcaagca
    20401 ggtttagcca ttgcttttcg tgaagacagt tcggttttag tagaagaatt tatgccggga
    20461 acggaatatc gtttctttgt gatcgacgga gaagtccaag ccatcatgtt gcgagtccct
    20521 gctaatgtta ttggtgacag tatccgaaca gtgaaagaac ttgttgaaga aaaaaatagt
    20581 gaccctttgc gtggaacgaa tcatcgtgca cctttagaat tgatccaatt aggtgaatta
    20641 gaacaattga tgttgaaaga acaagggttg acgatcgaat cagttcccca agccaatcaa
    20701 attgtttatc taagggagaa ctcaaatatc agtacaggtg gtgattcgat cgatatgact
    20761 gatgaatttt ctgaagctta caaaaaaatc gccgtttctg cggtagaagc actaggagct
    20821 aagattagtg gtatcgattt gattatacca gataaagaaa tcgatcctac aactgataaa
    20881 aaagcatatg ggatcatcga agcaaatttc aatccagcca tgcatatgca tgtttatcct
    20941 tttgcaggaa aaggcagaag attgacgatg aatgtattga aattgttata tcctgaagta
    21001 ttttaaaaga aaaagctttt gacagattgt cctatttgat gtcaatctga tcaaaagctt
    21061 tttttgcatg taaaaattta tgtgtttttt cgttttaaaa taaacgatcc cagaagcttt
    21121 ttttcttttt tggtttttct ttttcagctg gcgaagagtt ttcttctggg tatttttttt
    21181 cgatatcaat cacaggttca tttactgctt cttttgctgt taagagcaat ccgaattcct
    21241 caggatttgc agtgacgtaa tcgttgacta cagtgaaacg gctattgttt ttcgttgcta
    21301 gctggatata ttggttttgg acagaaaggg gtagagcccc attcaagagt atggaagcat
    21361 ctggatgttt tttgagttct tttaaaaagt tttctttgtt ttttggttgg atcatttcag
    21421 caacagtcat actaagatag cagcgttccc tgaatgtccc taaatattta tgctgttctt
    21481 cagggttcac taaaggtgta ccataaagtc ccttatccaa atggcttttt acatcatctt
    21541 gtgccataaa aaataccctc cttcttctct ttgtctttct tacgattata gcatattccc
    21601 aataatacct aaaacatatg aaaaagcggt tgcttttgtt caacgattca cttaaaataa
    21661 gaatgaaaat cattctcaat tagaaaggtg gattggatgc gctacggacg actagtagca
    21721 ttattagtta ttttaagtag tatttcattg ttcatcggag tatcgaatgt acaattgtca
    21781 gaactgctcc agttagatgg gcaaagctta caaatcttgc tagtgactcg tttgccacga
    21841 acgatttgtt tgatccttgt gggagcgacc agtagtattt gtgggttgat catgcaacat
    21901 ttgacccaaa ataaatttgt ttcaccaacg actgcgggca cgatggatag tgcgcgttta
    21961 ggtattttag tggctatgat tttcctacct ggtgcatcct tgattgtccg atccttgaca
    22021 gcattttgct ttgcttttgc aggaacattg ctttttttat cccttacgcg attgttcctc
    22081 cagaaaaatc aattgatgct tccgttgatt ggagtcatgt tagggaatat cattggttca
    22141 gttgctactt tttttgctta tcaattccag ttggtacaaa atatgacttc atggcttcaa
    22201 gggaattttg cgactgtgat acgaggaaat tatgagttat tgtatgctgt tgttccattg
    22261 ttgctcttgg tttggctatt tgcttaccga tttacgatca ttggtctggg cgaatcgttt
    22321 gcagtaaatc tgggaataga ttaccagaag atgcagtttt taggcattgg cttagtatca
    22381 ttggctagtg cagtcatgtt gatcatggta ggaagcattc cttttatcgg agtgatcatt
    22441 ccgaatcttg tctctagaat atatggagat caagtgcaca aaacaattgg cgtaactgct
    22501 ttagcaggta gtttgttctt agtattttgc gatcttgtcg ctcgcgtgtt gatttttcct
    22561 tatgaaatac cagtcagtgt gatcgttgga atcatcggtg gggctatctt cctttatctt
    22621 ctgataaggg gacgacgata tgcttaaaaa aaagccatct atttttattg gtatgcttgt
    22681 tcttgtttta tttgttacag gaatgtactt gacgatccat acatatggaa actggcattt
    22741 tgcatgtgca ttgaggagta agaaaatttt tgcatttatc cttgtctcgc tagctgtcag
    22801 cagtgccaca atcagctttc agacaattac gcataaccag tttttgactc cttcagtatt
    22861 gggacttgag tcgttatatg tccttgtcca aacggtcctt ttcttcttta tcggaggagt
    22921 gacgatgctt cagcagacag gtacagtttt tttcttattg aacgttgggc tgatggtcgg
    22981 gctgagtcta ttgttatttt tctttctttt gaaaaaaatg agagcgaatc tttttttatt
    23041 actgatgtca gggatgattt taggtacgct ttttggtagt gtcagtacct ttttacaagt
    23101 catcatggat ccgaatgaat atgatttgct tcaaggcaga ttatttgcaa gctttggcaa
    23161 tatccagagt cagtatcttt tgcctgcttc tttgatcatt atcggctgct ggtacctgtt
    23221 atttcgatat cgacatgtgc tagacgtttt ccatctcgga aatgaacaag cagtaaacct
    23281 cgggatagac acagatcgtt ttgaattttt gattttatgt tgtgtcagtc ttttgacagg
    23341 gacatcaacg gctcttgtgg gtccagttac ttttttaggg tttattgtcg caaatatcag
    23401 ttatcgtgtc ttttctactt atcgtcatgg tcagctattt atcggaagct ttctggtagc
    23461 tatgctgttt ctgacaggag gacagctcgc agtagagcag attttcggct ggaatacaac
    23521 agtgagtgtc gtcgttgaat ttgtcggagg aatttatttt gtcacaaaat tgattcgcga
    23581 aaggaagagt atcctatgat tgatttacaa aaggtcaata aactgtacca tggaaaatca
    23641 gtggttaaag atgtacagtt aacgattcct gaaaagaagt tgactgcttt tatcggtcca
    23701 aatggtgctg gaaagtcaac ggtgttgtcg atgatcagtc gtttgatacc aaaagataca
    23761 ggtgaaatct atttagacca taacgaagta aaggcttgga aatccaatga attagccaaa
    23821 aaattatcga tcctacgaca atcgaatgaa ataaatgtcc aaatcaccgt gaaagaatta
    23881 gtagaatttg gacgatttcc ttatacgaaa ggaaaattga cgacaaaaga cgaggaaatc
    23941 gttcaggaat cactggaaca ccttgggtta gaagaaatag cggatcaatc gattgacact
    24001 ctttcaggcg gtcaactcca acgtgcgtat attgcaatgg tgctagcgca agacacggat
    24061 tatattttac tggatgaacc tttgaacaat ctagatatga attttgcggt tcaaatcatg
    24121 cagattttaa aaaatttagt acaagaaatc gggaaaacaa ttgttattgt tcttcatgat
    24181 atcaactttg ctgcaagtta tgcagacgaa attgttgcaa tgaaagatgg acagttattt
    24241 acgcaaggaa cgacccaaga aatgataaaa aaagatgtat tggatgaatt atacgaaatg
    24301 gacatacgga tttgtgagat tgaaggccgc agattctgtc tttatttcta aaactaacat
    24361 taggagagaa gagatgaaaa aattagtagt aggtatcttg attctagcat catttggttt
    24421 agcagcatgt ggaaatagca acaacaccag tcaggcagac acaaaaaaaa gcagcacaca
    24481 aactgaaact acattgacga taacagatag taatggagac caaatcgagg tgccaaataa
    24541 tccagaaaaa gtcgtcgtat tcgacaatgg ttctttagat acgatggatg ctcttggtgt
    24601 tggagataag gtagttggag cagctacaag cagccttcct gagtatcttt catcctataa
    24661 aaaagtagaa tcagcaggtg gaataaaaga accagactta gaaaaaatta atcagcttca
    24721 gccagatcta atcatcattt ctggccgaca aagagatttt caaaaagatc taagtcaaat
    24781 tgctccgacc attttccttt ctttagatgc gaaaaatcca tgggaatctt tccagcaaaa
    24841 tgtcactgct ttaggggaga tctttggtaa gcaagaagaa gctaaaactc aattagagga
    24901 attatctagt gcaatcgatc agacgaaaaa gaaagcagaa gctactgaca aaaaagcact
    24961 agtcacactt gtgaatgaag ggcaattatc agcctacgga tctggttcta gattcggttt
    25021 catccatgat ttatttggct ttgaacaagc agatgatcaa atcgaagcat ccactcacgg
    25081 tcaaagtgtt tcttatgaat atgtgctgga aaaaaatccg gatatcttat ttgtagtaga
    25141 cagaacaaaa gctattggtg gagacgacag caaagatgat atttctgcca atgaactggt
    25201 agcacaaacg aatgctggta aaaatcagca gatcatctcc cttgaaccag atgtctggta
    25261 tttaagcggg ggcggtttag aatcaatgaa actgatgatc gaagatgtga atcaagcatt
    25321 taaatagcat aaataacaga ggattaaact ctcatactat gtaacaataa tgtttcttac
    25381 attcttaaat agcaagagcc tatcctcaaa gaagttatca aaaaccatcc gtaaaatcgt
    25441 attgatgatt ttacggatgg tttttattta tttgagaaat cttttttcaa cgtgaattca
    25501 ttatctacat catacgggtt gattctagaa tggggagtgg caaaaaatag tgtgatccat
    25561 tttttcagca tcaagatcgc ctcctttgat ttgaatcaaa ggtacaaagc gaaacttaga
    25621 taaaacttaa atttagatat ttttattttg taccgatctc ttctttaaac gatgttccat
    25681 ctgctgcttc tcggcaataa gtccactaga ctagaaaata tgagaagcta ttttctgtcc
    25741 agcgttctta ttactcaaaa caaatcgcag ttgtcacaat ctcttctaaa cgatgttcca
    25801 tctgctgctt ctcggcaata agcccactag actagaaaat atgagaagct attttctgtc
    25861 cagcgttctt attactcaaa gcaaatcgca gttgtcacaa tctcttctaa acaatgttca
    25921 atcagctgac cttcggtatt agctcaaagc actgatttca caacctctat ttaggcatgt
    25981 gtctcagcaa gcggttgata gttgatgatc tgcatttttc gataaaaatc ggcgggaagc
    26041 ttactccacc gttttagtaa ttgatagaaa taactctctt ctgctttgat tttatactcg
    26101 cttaatatca atccaaaact attcgttttt tttgaagcta attttttata aatatccaat
    26161 gctttggttt tatcttcatt gacaaagagt gtttttactt caatataaga accctcgata
    26221 ttttgtgttt cttcgataaa atagatgttt tttatatatt ccttcatagt gattcctcca
    26281 aagaagtctt ttatttgata agtttactct accgaagagt tgttaataat ccgtgaacaa
    26341 aaagtaattc tttttccaat agttgttaaa ggttttttat tttttatagt attttttgtt
    26401 atttaagaaa ggaataaggt taaacaaata acaggttgct ttttgggtct ttttccgttc
    26461 aagaaactga acgaagaaaa gggaaagttg tctcttttgt gataagagaa atcataataa
    26521 acgaatgact tactttccaa taaattgaga acagtcagaa ccagacaaat tagttgcatc
    26581 tgatgaataa agtgattatt ctatgacatg aggtgataca gatgaaatta gaagtgacaa
    26641 aagaagcaca agaagtattg aagaataaat tagaagcaga cgatcaactt ttattagata
    26701 tcgaaaacgg tgacggtcca tttgcagaaa gccaagtcag ttgccaattg gatacggcat
    26761 ttcgattgat cattgtcaaa aaagatacac aaacagatct aagtctttat tctgtagtgg
    26821 ctgatacacc agttggacca atcaaaatca aagacagtgc aatactttac atggatgatc
    26881 catccacact tgctttagaa ccaacttata atagtttaca attaaaagga ccaagcggct
    26941 tgagaaaagg caacttgcaa gttgttcgaa aagactagag ggataaaatg ggaaaattgg
    27001 cgatcatcag cgacctacat gtggacatca ataaattcgg cgaatcagag ctgatgcagc
    27061 tttggcaagt tttgcaaaag cacaagatca ctcggcttca tctagccgga gatacggcta
    27121 ataaagtaga ccgttgccta gctgttgtcg aatttttcgt caataaagga ttatctacga
    27181 catttcattt cggcaatcac gagttggcag atgtgcccaa tgaacagatg atggaccatt
    27241 ttccagaccc acatttttta aatgaacggt atattgcttt aaatgagcag accgttcttt
    27301 tagggatgaa tggctggtac gactatcagt tttccgaatt gaaagattac gaggcaatcg
    27361 tacgaatgaa gcgtttgtat tggtatgatc ggttcatcca gcgtgggaaa acagatctcg
    27421 aggtcaatgc agaaatgctt gcagctgcaa aaaatttgct agaccagctg gaagcaaaaa
    27481 aacttgaggt gattttatcc actcatttcg ttcctaaacg ggcatttatc atgtatcaat
    27541 ctgctcctta tgagcgttgg aacaaattaa atgcattttt aggatcagaa tcattcggag
    27601 aattacttga ccgttattca aatatcaaac aagtagtttt tggccatacg catcgtcgct
    27661 ttgaagataa atccatcggt cacacgattt acagctgcag accttttggt tattattatg
    27721 aatggcagct gacccgagat tttgtcttga aagaacagtt gctagacagc tatgatccta
    27781 tgaaattacg tgtcctcttg cgtgcccatc acccagcttt tcaggcctat caaacacaac
    27841 atttgcaaga ggaatttgaa caagcgatga caatcgttcc gtattgagac agcattagta
    27901 atgtgtaaaa gaaaatacct tgtaaaacat aaggataagt aagagcagta gatccattta
    27961 tcgtggatat gctgcttttt ttgcggaaaa taatcagcga atgtttattt gatcggatga
    28021 tttgtaactc gcagagtact agatgcaagc gtgtattctt tttttgaacg ataatcacaa
    28081 aaaaaaatat aatatacatt ttttatttaa ttataattaa tattgattga agtctaggca
    28141 gtcaagtcac attttgatag ataggagggg gacttacgaa ttattttaaa ggctatgaca
    28201 gctgtatttc tagattgtgt ttgcatcaag ttgcaataaa aactagaaat caaatcgggc
    28261 tgataatcat ttataactaa tcgatacata ggaccaaata taggtctatg ttcttttatt
    28321 tcataagtat cgtaaaacag aattttgcta tggattaatc caatcgactg atatgtttag
    28381 taaataaaga aagaggaatg tcaatgatga aaaaagaaat cgtattaaca gcagcaatga
    28441 tgttattcag catggtagca agtacgactt ttgtttctgc aactgaagta tacccaaagg
    28501 aatataacac agaaggaacg attacatttg aagcaggaga tgagggtgta actcctcctg
    28561 ttgatccaga gaatcctgat cccaataaac ctgtcgatcc atcagatcct ccaagccctg
    28621 gaacaggagg ggcattatcg attgattatg gatcaaaatt caaatttggg actcaaaaaa
    28681 tttctactgc ggataaaacg tattatgcag ctgctgatgt gatgaacgat gggtctagaa
    28741 aaccgacata tgtccaagtg acagatagga gaagtacctt atctgggtgg aaattaagtg
    28801 tctctcaacc agaacagttt aaaactgcca gtggggatga actagttgga gcacagctga
    28861 aatttacgaa agggcaggct gtttctttgg ttgatccaac atatacgccg caaacggtga
    28921 attctgaatt aacgttgaca ccgggaggaa acaatacgtt agcaattaat gctaaatcag
    28981 gaacaggtgt ggggacttgg gtctatcgat ttggtgcaaa tgaaaatgaa aatcaagatg
    29041 cggtacagct ttcggtaccc ggtaaatctg tcaagttagc gcaacaatat tccactaaat
    29101 tagtttggac attagaagat acacctaaca actagtttac tgaaaagaac tgttaaaaat
    29161 aaatacgtag tcatattgtc ttgcgaggta atcgagcaaa aattgtcaaa ggctaggcta
    29221 aatttgtttg gatacgaata gttcatctag gaatttaacg atcaggagga aaagatatat
    29281 tgaaaaagag gaggatcgtg ctcattttaa gcagtctctt agtgactaat atctttccga
    29341 tcatagggta ctctttggaa ttgaatcagt tggagcatgg aaatagaaga gacgaagaaa
    29401 gcaaactcac ttcgacattc gacagtttaa tagaatccaa ccagacacaa gaaagcagtg
    29461 aggatttaag tatttcgagt agtggagaga ccactgacaa cgtagaagct gagggttcgg
    29521 acaaacaagg actagaagat gcaccaattt ctgcggttga gctgcctgac gatcccaata
    29581 ttatttctat cgataaaatt tttcaaacac ctatcggtac tagcacgtcc atattggaaa
    29641 atgggaagtt actgcagttg aacccagcag aaaggtctca aagaggagct atttggtcaa
    29701 ataagaagat tagtttactc tcggatttta catttaaatc ttatttatac ttaggaaatc
    29761 aacgagatag agcgggagat ggcatgacct ttacattgac tggcgatcct agaatggcta
    29821 gtaatcccag tcaagttatt ggatcgccag gtatgggaat tggctcatat tctactcgtt
    29881 ctggccaacc ttacattgcg aatgcgctct cgattgagtt tgatacgtat aaaaatagtg
    29941 gttcttctga tcgaatggat cgagagataa ctgctgataa tagagggcat ggacatgtcg
    30001 cttttgtcac gcctaaacca aataataata attattctgg agagcatact ggcgtgacgg
    30061 tagctcctac atttctttct aatggcacat ggcgcttgtt aataattaaa tgggacgcgg
    30121 agcaaagaag tcttacttat gatttggaag gtgtcggtcg ctcttctcgg attatacctg
    30181 atctcattag gcagtttggt tctacagaag taaattgggg gtttacttct tcaacaggta
    30241 ctttttttca agaaaatgct gtcgctgtca cgcgacttcc atcgaatgtc acatcaacag
    30301 ctgatgtaaa agtcaacgat agtgattatg gggccatcgc tgaagctgga ggaaacgata
    30361 agataactat cagaaataca ttgaaggtcg agggggattt tcttgaatat aagaaaatgc
    30421 agactagtat cgacctccca caagaattaa ctattcccga gcatatcttg atcaatggaa
    30481 atcaaattga tgccaaagat atcttgataa ccggaaatcg agtcacgcta aatctgacag
    30541 attattttat tgctggagat acgttaataa tcgaattgga gacaactttg caggaagtaa
    30601 ttcctgaaaa agtactcact atgcattttg aataccttga aaacgggaat ttgattgagc
    30661 aatcgaatga aataaaccta aagatcgcaa agctgaagga gatagatatc catgtttatt
    30721 ataaagataa agacagtcag attgatattg ctgaaatgaa aacaatcact gggaaaatag
    30781 gagagaagta taaagaaact cctattgata ttgaagggta tgtttttgac tcggattcag
    30841 cgaatacaga aggcatcttc tccgaaaata cagaggatat ctatttttat tacagaaaag
    30901 gcaaacttta tctctcaact gctcctcaat atgtcaattt tggaaaacat aaaatatcca
    30961 gtagaccatt aagtgaattc ggacagtttt caggtgggtt gaaaattata gatgaacggg
    31021 cttttggaag ttggaaatta caattgaaac aagagaaagt tcttacaaat ggagattttg
    31081 agcttccaga agctctttcc tttgttacct ctgaaaatac aacggttata gggacatccg
    31141 ctgttacgat tttcgaaagt gatcaaaagg gtgaaagcga tttgtcctct cttttagatt
    31201 ctacagctca tcgtggaatc agaataaata ttcctgtaga aaaccaaaga atcggaacct
    31261 ttgaggggaa actatcttgg atacttgcag atgtcccagg aaattaacag gaagagaaga
    31321 atataacaaa attattgagt ttcaagaagg gcacaggtac cataggctct tgtgtcccac
    31381 agagacaaga ttttgttata gtcttctctt acatatgatt aaaggagcga ttttcttgag
    31441 accaaaacaa caattattta taacactcct agttatttta ggggcacttt ttaacaatat
    31501 atcagtgtca gctagtgcat ttaatttttc agtgacgccg ataacttcag agaatcaaat
    31561 cgacaagaga aaaacttact tcgatcttca acttgatccg gatcaggaag tagaagtaaa
    31621 agcggaattg agaaatgata cagaaaaaga agtgaaaatc gatatttctg tgaatagcgc
    31681 aaccaccaat tcaaatgtga tggttgaata cggaaaaaac gagattgaaa aagacatgag
    31741 cctgatcttt gatctggtag attatgtgag ctatccacag atagtgacgc taaaacctaa
    31801 aagtgtccaa accgttgctt ttcatgtcag gatgccgaat gagcgatttg atggtgtatt
    31861 ggctggtggg atcacattcc aagaaatcca gacggaaaag gaccaaacag aaactaagga
    31921 tcaaagctta tcgattgaga atgaatacac ctatattatt gctttgttga tgcaacagac
    31981 actgaacgaa gttgctccga accttactct tcatgaagtt aaaccagatc aaatcaatgc
    32041 aagaaatgtc atcttagcaa acgttcaaaa tgatcaaaaa acctatatca atcaagtagt
    32101 tattgaaaca aaaattacaa aaaaaggaca ttctgaagtt ttatatcaag aggaaaaaga
    32161 aggattgcag attgcgccga atacaaattt ttcttttcca actgctttaa atggtcaacc
    32221 gctaacccca ggtgaatatc accttacaat gactgtttta ggaaatgaaa atgaaaatgg
    32281 aaaattttcg agaaaaaaag gaaatactac gatcaactat accaatcaat gggtatttga
    32341 aaaagatttt acaatagacg gaaaagtagc caaggaattg aacacgacgg atgttacgat
    32401 taaaaaagac aataattgga tttatatctt agtagcttca cttttcttat tgtttatttt
    32461 attatgcata cttttgtttg ttttgagaaa aaaagaagag aaagaaaagt aaggttaaga
    32521 taacaaatag taaattttta ttattgtagg atggttgggc tctttttata acaatataaa
    32581 atataccatt ttattaataa aaatgcatac tagtaaatga tataattcta ataacaggtt
    32641 tgatcgcgag aaaggccaaa taaaacgctc tccaaaatgt taaaaaaaag acaggacgat
    32701 gtatattata caaacaagaa tcgctgacaa cagtgactcg gatccaggaa atcagtttta
    32761 tccccagcta gttggtgaat gcatggaaag taatatacgt agaaagagct gatgactaat
    32821 gaattatgag ttttttttaa gcaaagaaga tcacagaagt tattcattac ttaaatattt
    32881 agaagcaagc tcaaatttat cagagtcaat atcgaatgtt caagaagagt tgtcgctatc
    32941 gacatttctc ttgaaaaaaa cgatcgataa gctgaaacat gatttacaaa agttcgatct
    33001 agagaaaaat ttgcgagtaa ccgtcagtga attggatata tgtctggaaa ttaatggaaa
    33061 atgttctagc aaagctctgt tatctcgtta tataacggat tctttatcta tgaaaatggt
    33121 attggctttc tttcaggaaa aatatatctc agtagaaaat tttgctaaac agcatcacgt
    33181 gagttattct gtggcttata aagtattaca aagactaaaa agaaatctaa aaaaatatca
    33241 aatttttttt gaaaagagaa agttgacgag agtataaaat attttgtgta aatgaaaaaa
    33301 tccatacaaa aaaggaagtc ccttctgtag aataaagtta acgacaacca attcacagaa
    33361 aagaggactt ccctatgaat gattttacta cagaaattgt gcaaactcta gtcactaaag
    33421 gcgatttaaa tgaattattc cgttcgcact tagaaaaagc gataaacaca ctcctacgga
    33481 ctgaattaac ggctttttta gattacgaaa aatatgatcg cactggtttt aattcaggta
    33541 attcgagaaa cggttcttac tttcgatcaa tcaaaaccga atatggtgaa ttaacattgg
    33601 aaatacctag agatcgtaat ggtgagttta aacaacaaac tttaccagcc tacaaaagaa
    33661 caaacgatac attggaaacc actattatcc atttattcga aaaaggtgtt acgatgtctg
    33721 aaattgctga tttgatcgaa aaaatgtacg gtcatcacta tactccacaa accatgtcca
    33781 acatgactaa agttctgact gaagaagtaa atgcctttaa atccagagcc ttaaatgata
    33841 agtatgtcgc tatttttatg gacgctactt acattccact aaaacgtcaa accgtatcca
    33901 aagaagcgat ttatattgcc attggtatac gagaagacgg cactaaagaa gtactgagtt
    33961 atgcgattgc tccaactgaa tcaacatacg tttggaatga gctgctacag gatattaact
    34021 ccagaggagt tcaagaagtc ttgcttttta ttacggacgg cttaaaaggc atgaaagata
    34081 ctatccatca aatttatcct aaagcaaaat atcagcattg ttgtatccat gtatctcgta
    34141 a
//
LOCUS       DANJMI010000032        32533 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000032 DANJMI010000000
VERSION     DANJMI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.032"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            131..505
                     /locus_tag="PWU81_001758"
     CDS             131..505
                     /locus_tag="PWU81_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL1110460.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="PWU81_001759"
     CDS             complement(619..1248)
                     /locus_tag="PWU81_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL1110461.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="PWU81_001760"
     tRNA            complement(1382..1454)
                     /locus_tag="PWU81_001760"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="PWU81_001761"
     tRNA            1668..1741
                     /locus_tag="PWU81_001761"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="PWU81_001762"
     CDS             complement(1879..2043)
                     /locus_tag="PWU81_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110462.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="PWU81_001763"
     CDS             2338..3666
                     /locus_tag="PWU81_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HDL1110463.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="PWU81_001764"
     CDS             3868..4701
                     /locus_tag="PWU81_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDL1110464.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="PWU81_001765"
     CDS             4717..5454
                     /locus_tag="PWU81_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HDL1110465.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="PWU81_001766"
     CDS             complement(5609..6577)
                     /locus_tag="PWU81_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL1110466.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="PWU81_001767"
     CDS             6809..7642
                     /locus_tag="PWU81_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL1110467.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="PWU81_001768"
     CDS             7676..8515
                     /locus_tag="PWU81_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1110468.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="PWU81_001769"
     CDS             8541..9101
                     /locus_tag="PWU81_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL1110469.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="PWU81_001770"
     CDS             9300..11021
                     /locus_tag="PWU81_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDL1110470.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="PWU81_001771"
     CDS             11186..12328
                     /locus_tag="PWU81_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL1110471.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="PWU81_001772"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="PWU81_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL1110472.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="PWU81_001773"
     CDS             14128..14775
                     /locus_tag="PWU81_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL1110473.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    14904..15120
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15168..17813
                     /locus_tag="PWU81_001774"
     CDS             15168..17813
                     /locus_tag="PWU81_001774"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL1110474.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="PWU81_001775"
     CDS             18123..19436
                     /locus_tag="PWU81_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL1110475.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="PWU81_001776"
     CDS             19426..20079
                     /locus_tag="PWU81_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL1110476.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20826
                     /gene="radC"
                     /locus_tag="PWU81_001777"
     CDS             20134..20826
                     /gene="radC"
                     /locus_tag="PWU81_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDL1110477.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20976..21176
                     /locus_tag="PWU81_001778"
     CDS             20976..21176
                     /locus_tag="PWU81_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL1110478.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21430..23109)
                     /locus_tag="PWU81_001779"
     CDS             complement(21430..23109)
                     /locus_tag="PWU81_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL1110479.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(23111..23323)
                     /locus_tag="PWU81_001780"
     CDS             complement(23111..23323)
                     /locus_tag="PWU81_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDL1110480.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23716..25446
                     /gene="efrA"
                     /locus_tag="PWU81_001781"
     CDS             23716..25446
                     /gene="efrA"
                     /locus_tag="PWU81_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDL1110481.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25443..27203
                     /gene="efrB"
                     /locus_tag="PWU81_001782"
     CDS             25443..27203
                     /gene="efrB"
                     /locus_tag="PWU81_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDL1110482.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            27298..27495
                     /locus_tag="PWU81_001783"
     CDS             27298..27495
                     /locus_tag="PWU81_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110483.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            27648..28133
                     /locus_tag="PWU81_001784"
     CDS             27648..28133
                     /locus_tag="PWU81_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDL1110484.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="PWU81_001785"
     CDS             complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="PWU81_001785"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL1110485.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            29010..29537
                     /locus_tag="PWU81_001786"
     CDS             29010..29537
                     /locus_tag="PWU81_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110486.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            29628..30365
                     /locus_tag="PWU81_001787"
     CDS             29628..30365
                     /locus_tag="PWU81_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL1110487.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            30369..31286
                     /gene="miaA"
                     /locus_tag="PWU81_001788"
     CDS             30369..31286
                     /gene="miaA"
                     /locus_tag="PWU81_001788"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL1110488.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            31288..32517
                     /gene="hflX"
                     /locus_tag="PWU81_001789"
     CDS             31288..32517
                     /gene="hflX"
                     /locus_tag="PWU81_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL1110489.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10771 a         5522 c         6874 g         9366 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaagt tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tctggtaatg ttattccatc gcagcaggat
    20701 atgaatttta ccaaaaggat acaaaagtgt ggagaaatga tggggatcac tgtactggac
    20761 cacttgatca tcggacgcaa acgatatttt agtttgagag aagaaggaat gatggaagag
    20821 aagtaagttg ctttcttgca aaaaaaatga agcaagtgta caattatatt gtaattttgt
    20881 ttgggaatag aagttgaaac atctgttgca cctctatcgt acacgagcaa tgttacaatt
    20941 taaattagtg cttttggcac gaggaggata ttattatgga aaacggaaca gtaaaatggt
    21001 tcaacgctga aaaaggattt ggatttatct cacgtgaaga tggaagcgac gtattcgttc
    21061 acttctcagc tatccaaggt gacggattca aaacattaga agaaggtcaa gcagtgactt
    21121 tcgacgttga agaatcagac cgcggcccac aagctgcaaa cgttgtaaaa gcttaatttt
    21181 atctgtaaat caaattgatg gtagacatca atttttacga gagaacaaag cgagtaacaa
    21241 aaatacctgc cacaagagac aatttctctt gtggcaggta tttttttgta tgaaaaagta
    21301 aataaattta agaggctatg aaagagccac cttgtatcaa ggaataagaa caaacaaaaa
    21361 tggcttctca tgtttttcat ttgttttctc ttatattcag gacaaggtgc tgatttcata
    21421 acctcttatt tattcttcat ctggtgtcat taccattggc aaaatcattg gatgtctttc
    21481 tgtacgttcg aataagaaag gctgaagcgc gccagtcatc gcttctttca atttatattc
    21541 tgaacagtca ggtccattca aacattctct taaagcgtgg aataagattc gctgagcttc
    21601 attgatcatt tctcctgatt ctcgcatgta gacaaatcca cgagagagaa tatctggacc
    21661 agccaagatt tcttttttct tgatatccac tgtcgcgaca gctaatacaa gaccttcttc
    21721 tgaaagcagg tgacggtcac gtaaaacgac attaccaata tcaccgatcc cgctaccatc
    21781 gatatacaca tctccagcca caaaatgccc agcaggacgc gcagaatcgc tagttaacgc
    21841 taaaacatcg ccattttcca tgatgaagca gttttctgca ggaacaccta catcttgtgc
    21901 tagagaagca tgtactttca acatgcggaa ttctccgtgg acaggaataa aatattttgg
    21961 cttcatcaaa cgaagcatta atttttgttc ttcttgtcca ccgtgaccgg aagtatggat
    22021 gttattgatt tttccgtgaa tcacttctgc accagcttca gacagcaaat tgatcaaatg
    22081 attgacactt gttgtattac caggaatagg tgaacttgaa aagatcactg tatcgtctgg
    22141 ctggatttgg atttgacggt gagtaccatt cgcgatgcgg ctcaacgcgg ccatcgcttc
    22201 tccttgagaa cctgtacata ggatcatcgt ttcattggca ggcaagcgat tgatttcgtg
    22261 tgcatctaca aatgtgccgt ctggaacttt gatatatcct aaacgttgac cgttgacgat
    22321 ggcattttcc atgcttcgtc cgaaaacagc gattttacga ccagttgcca cagcggcatc
    22381 tgctgcttgc tgcaaacgga agatgttaga agcaaagctg gcaaaaatga tacgtccttc
    22441 gatttttgaa aagatcttca aaatagaatg cccaatcgtt ttttctgatt tagtaaatgt
    22501 tggaatctca gcatttgtac tatcagataa aaggcacaag actccttctt ccccgatacg
    22561 agccatttta tgcaagtttg ctggttcgcc aacaggagtg aaatcgaatt tgaagtctcc
    22621 agtcgctacg atatttccag aaggtgtttt caccacgatc cctaatgcat ctgggatact
    22681 atgggtcgtt ctgaaaaagc tgacagaagt ctttctaaaa cggatgatcg tatcttcatt
    22741 gatctcatgg agctctgcat ctcgaagcaa gccatgctca tctaatttgt ttgtgatcaa
    22801 cgctaatgcg agaggaccag catagatagg aatatttgct tgtttcagca agtagggtat
    22861 tccgccgatg tggtcttcgt gcccgtgagt aatcaccaag gcttttactt tgtgtaagtt
    22921 ttgtatgatg tagctgtagt cgggaatgac atagtcaatt ccaagtaaat cgtcttccgg
    22981 gaatttgatc ccggcatcaa tcaaaatgat ttcatcttgg aattgtaccc catacgtatt
    23041 tttcccgatt tcacctaatc caccaatagc aaagacgcct gtctcgttat ttttgatgga
    23101 aagtttcata ttaaaactcc actaatgaga aatcagcatt ttctttttcg tactctagat
    23161 gacttccttc tagaagttga acgaattcaa tattgtatgg ggtattttct tccacacgtt
    23221 ggcgagcgtc tacatcgctt tttgcctcaa tgtaaagaga ttttgtttct tcccgtttag
    23281 ggttgcgtac ttttgtttct tgataataaa ctttatagat cattgttttg tctcctttat
    23341 ctgcgtaaag cagtgattta gctagttgat ttttttagac aacacaaaac gaggggacaa
    23401 gccacctggt ttttcatatg ctttgactag aatactggat tcgagtcaaa gagacaagcc
    23461 tcgtttttgc ttatctgttc ggatattttt tcaaccgtac cttctgtata aaaaatatac
    23521 aacaattaat tgttattcta gcataaaaca gcaaataagt atagaaatca actagattga
    23581 aagaaaaaat caatgtttat catcccgcta taatcataat gactttttta tgagagaagt
    23641 gttctattaa taggctcgtt gtgctatcat ttttagtaat gaaaatgtaa caaaagatta
    23701 aaaggagatg agaaagtgaa actaatgtgg cattatacca tgcgttataa gaaattcttg
    23761 tttttcaatt tcatatgcgt atttggtttt attttgattg aattaggact tcctacgatt
    23821 ttagcacgta tgatcgatgt aggaatccgc aatgatgatt ttgattatgt gaagcaacaa
    23881 gggatattta tgatcgttat tactgtaatc ggtattattt tgaatatctt gttaggttat
    23941 tttggttcga ggattacgac taatatcgta gcagatattc gagatgattt gttcgaaaag
    24001 atccaaagtt attcgcatca ggaatacgaa aatctaggcg tatcttcatt gatcactcgt
    24061 acaacaaacg atgcttatca gatcatgttg ttcctgcaaa atattttacg tatcggtttt
    24121 atggcaccaa tgatgttcat tgtcagtttg tacatggtca tgcgtacaag tgtgacactc
    24181 agtttgtatg tagtaggtgc attaccttta ctattattgg cagtcgtagc gattgctaag
    24241 gtgtctgaac cgttatctaa aaagcaacaa aaaaacttag ataaaatcaa cagtatcttg
    24301 cgggaaaatc tttcaggatt gcgtgtgatt cgtgcattcg tcaatgaaaa gtttgaagaa
    24361 aaacgttttg ctgtagtcaa tgagaattat gcttctagtt caaaaagctt attccgtttg
    24421 atggctgttg cacaaccggg tttttatttc ttatttaata tcgtaatggt attgatcatc
    24481 tggagcgggg cagtgcaaat cgatgcaggc gagcttcgtg taggagattt gattgcgttt
    24541 atcgagtata tattccatgc cttgttctca ttcatgttat ttgctagtgt gttcatgatg
    24601 tatccaagag cagctgtttc agctggacgt atccaagaag ccctagatgc caaaccttcg
    24661 attgtagaag atccaaatgg tatatctgat acaaaaacta aagggtatat cgaatttcaa
    24721 aatgtaacat ttgcttatcc aggccattcg caagagccag tgatacgaaa tgtcagtttt
    24781 acagcctctc caggagagac cgttgctttt attggaagta ccggtagcgg aaaatctacg
    24841 ttgatccaat tgatccctcg cttttatgat gtgagtgaag gaaagatctt gatcgatgga
    24901 gtagacgtga gagattatcg gctaagcaag ttgcgagata agattgggtt tattccgcaa
    24961 aaagcgctgc tatttactgg aacgattgca gataatttgc gatacgggaa agaagatgct
    25021 acgcaagaag aaatggaacg tgcagctgag attgctcaag cttcagaatt catttctcga
    25081 aaaccagatg gatttaatga acatttatca gaaggtggtt ccaatttttc aggtggacaa
    25141 aaacaacgat tggcaatagc cagagccatc atacgccgtc cggaaatcta tatttttgat
    25201 gacagctttt ctgcattaga ttatcaaacg gatgcgaagt tacgtgctcg tctgaaaaaa
    25261 gaaacgacag aatctactgt attgatcgta gcacaacgtg tgggaacgat catgcatgca
    25321 gataagatca tcgtattaaa tgaaggagaa gtcgtcggta tggggacgca tcgggagttg
    25381 ttggaaaaat gcccgattta ttatgatatt gccgcttctc aattatcaaa ggaggaatta
    25441 gcatgaaaaa aaccttttct tccttaaaac gtttagctcg atatatcaga ccgtatcgtg
    25501 agacttttct tttagtgatc ctatttacag ttctgacagt ggctttcaac gtggctttgc
    25561 catatgttac aggacttcct actacagaaa tcagccgtaa tattgccaat ggcgaatcaa
    25621 tcaatttttc ttatatattc aaatgtctca tttggatcac agttgttgga atagggtatt
    25681 gtatttcgca attactttct ggtgttttga tgacaaatgt cgttcaaagt gccatgcatg
    25741 atttgagaag agacatagaa gaaaaaatca atcgtcttcc ggtttcatat ttcgataaaa
    25801 accagcaagg aaatattttg tctcgtgtga cgaatgatgt agatgcagtc agcggtgctt
    25861 tacaacaagc ttttattggt atcgtaaatg cgatattggg aattacgttg gctgcagcaa
    25921 tgatgtttta tatccagccg atgatggcgc tgatctctat gatcatgatt ccaacatcga
    25981 tctggatctc aaaacgagtg atcaatgctt cgcagaaata ttttcaaagc atgcagaatt
    26041 ctcttggtga gctaaacggc tacgtccaag aaaatatgac aggttttagt gtattgaaag
    26101 tatatggacg cgaaaaagaa acattggaag gattcaaaaa agttaatcac agcttgaaat
    26161 actttgggtt tcgtgcagca ttcatttctg gcttgatgat gcctctagtt caattaactg
    26221 cctatgctac ttatattggt atggctgttc taggaagctt ttatgttatt actggggcga
    26281 tcgttgtcgg tcaactacaa gcattcatcc aatatatttg gcaaatcagt cagccaatgg
    26341 ggaacgtgac acaattgtct tcagttttgc aaagtgcatc agcggcaacg aaacgtgtat
    26401 tcgagatttt agatgagcca gaagaaaaag aaaacaaagt agatgcttca ctgccagaaa
    26461 cgattttagg agatgtgaca ttcgatcatg tcgattttgc atatgatccg aagaaaccat
    26521 taatccaaga tttgaatttt gaagtgaaag ctggtcaaac cgtagctgtg gtcggtccga
    26581 ccggtgccgg caagacgaca ttgatcaatc tattgatgcg tttttatgac gtagacaaag
    26641 gagcaataag gattgatgga atcgacacca aatcaatgag tcgcagtgat gtgcgttcgc
    26701 tatttggtat ggtcttgcaa gatgcatggc tttatcaagg aacgattgca gataatatcc
    26761 gcttcggaaa gcttgatgcg acagattatg aagtagttga tgctgcgaaa acagcaaacg
    26821 tagatcattt tatccgaacg atgccagatg gatatgatat ggccatcaat tctgagggag
    26881 acaatgtgtc tttaggacaa aaacagttgc tgacgattgc ccgagcagtg gtctccgatc
    26941 ctaaaatctt gattttagat gaagcaacaa gttcagtaga cacccgttta gaagcgttga
    27001 ttcaaaaagc aatggatcgc gtgatggagg gacggacaag tttcgtcatc gctcaccgtt
    27061 tatcgacaat ccgtgatgca gatctgattc tagtgatgga tcaaggtcag atcattgaaa
    27121 aaggaacgca tgagagttta ttggcacaag gtggatttta tgaaaaactg tataacagcc
    27181 aatttgctga ggaagcagaa taagtcatat aagaataaaa cagtttaaaa cttatttttt
    27241 gttctctatt cttgataatg gtaaagtaaa gttatcaaga aaagtgagga cacggagatg
    27301 gaacagtttt taaatgaaaa aattactgaa gccaaaagag aagttgatcg tttaacgcac
    27361 gtctgtcaga cgaagccttt tgattcagct gatttttttg aaaatgagtt tcaaatcgaa
    27421 cgcttggcag cacaaataga agtgtacgaa ttaattttaa aaaaagtaat agaagaaaat
    27481 atagatgtac aataaaaaca ggtaaaacgt cagttaccag atactgtaaa tagacatctg
    27541 gcctgagcag gttttacctg ttttttttct tatgaaataa atatatgcta tgataccttt
    27601 attcaatagg attagtttct acaaaaaatg aaaggagaaa aataaatatg gaacggttta
    27661 tagcatcaaa tgccacagtg attggtgatg tgacattgtc agaagatgtg acgatctggt
    27721 atcaagctgt tttacgcggg gacagtaatt ggataaaaat aggacaaaga acgaatatcc
    27781 aagatggtac aatcatccat gtcgatcatg atgcacctgt agatattgcg gagaatgtga
    27841 cggtaggaca tcagtgcatg ctacatggct gtacaatcga aaaaggggcg ctgatcggga
    27901 tggggaccac gatattgaat catgcggtta ttggtgagaa tagtttgatt ggtgctggat
    27961 cattagtaac agaaggaaaa gttattcctc ctaatgtctt ggcttttggt cgcccagcta
    28021 aagtgatccg tcctttgaca aaagaagaaa tacaaaaaaa caaagaaaac atccagcatt
    28081 atgtcgagat aggtcggatg catgcgcaag gaaaattcaa ggagcaaaga taggcagtag
    28141 catccataaa ataaaaaagg atggtcagtt tgagttgacc atcctttttt atttggttta
    28201 gataagagag cttaaaaaag cttcctttga aacagattaa tttaatgaag aactgttttc
    28261 catgacttca tcgatcaatc catatgcttt tgcttgttca gctgtcatat agttgtcacg
    28321 atctgtatct ttttcaatca cttcaagcgg ttgtccagtg cgttcagcta aaattttgtt
    28381 taagcgttcg cgtgtttgca aaatatggcg tgccgcaatt tcgatttcag ttgcttgccc
    28441 ttgagcgccg ccaagaggtt gatggatcat gatctcagca tttggtaaag caaatcgttt
    28501 accttttgta ccagctgtca gcaagaagct tcccattgaa gcagccatac ccataacaat
    28561 tgtttggaca tctgctttga caaagttcat tgtgtcgtaa atagccatac ctgctgtaac
    28621 tgaaccacca ggagaattga tataaagata gatatctttt tcagaatctt gagcgtctaa
    28681 gaaaagcaat tgggcgatga tcgaattagc aagatcatct gttacttggc cgcttaacat
    28741 gatgatacgg tctttcaata aacgtgagta gatatcgtaa gctctttcac ctcgtgaaga
    28801 ttgttcaatg actgtaggaa ttaaattcat aatagagttc ctccttgatt tcgtttagaa
    28861 atagtgtacc ataatttagt ttctaatacc taaaatgtag tatacatttt tggtcaaaaa
    28921 aggtcaaata aaaaacttag gcaaattagt cgcataaacc cgctttatgg tatgataagc
    28981 agagaaacaa aacaaggagg aacaacagat tgatagttgg tattactggc cttatcggag
    29041 tgctgacagg tgctgtagtt gtctatatcg gcatgatact gcaaatgcgt cacttggaaa
    29101 ataaagaaca gcgtagacgt gaattggaat tgaaagtcaa agaaatcgag acgctgaatc
    29161 aattaaataa aaagatcaac gagatcttgc aaaaacggac aacgctgatg gaagaatatg
    29221 tcagctttga tgcatttgat gattgttata tcacgattga cgattttgcg tatctccaat
    29281 cattttcagc gcaaaatagt ttttatctgc caaattatat tttagacgag ctatttaaga
    29341 atatctccca tcgaaaagtg attttatctc cagatgaaac aatgaagatt ggtggatatg
    29401 cttataaagg cggacgcgtg attttagaga attttacgga tagtctgact gaaatgatca
    29461 atgaaaaaaa agctcagttg aagactatga cgcgccagcc gattggtttc ttctcgaaag
    29521 atgatgtttg gagctgatat tttgaagcat tgccgttgca catgagagcg tggtaatgct
    29581 ttcttttttt cttaatgata caatagaact gaacggagtg aagaaagatg ttacaaatct
    29641 atgcccatcg cggaagcagc ggcacccacc cagaaaatac gcttcctgca tttgcagaag
    29701 ctgtacgagt aggtgcagat ggtatcgaac tagatgtcca tctaagcaaa gatggttatt
    29761 tgatcgttat gcacgacgaa gaagtggacc ggacgactaa cggtaaaggg ctgattcgag
    29821 aaaaaacatt ggaagaactc aaaaaattga atgcaggggg caaattcagc aaagaatttc
    29881 cagcagctaa agttcctgaa ttgagagaag tgttaggact gctcctccaa aaaaattacc
    29941 gagggatgct aatgattgaa attaaaactg atcagtatga atatcccggt atcgaagaaa
    30001 aattagccga aacattaaaa agtagagatt ggccttttca acatgcatat tgcagctttc
    30061 atttggattc tttggaaaaa atcgcattac tggaaccgga gatacagctg gacttattga
    30121 tgagtacttc tgataaaaag atccaactag cagcagaaac cccttatata gaaggagtac
    30181 atccaaaaaa tacttggttg ttcgaacatc cagtccaggc agctgatttt ccaaaagcaa
    30241 ttcgtgcatg gacgattaac gatgaagaag aaatggccaa atgtcaagaa ctcaaaatag
    30301 atggaataat aactgatttt ccggagaaag ctcttcggtg gaaacaatca acgaaaagta
    30361 ggtaaaatat ggaaaaagta ttagtgatcg tcggtccgac agcagtcggt aaaacagcgc
    30421 taagtgtgga actcgcacaa ctatttggag gagagatcat cagtggtgat tcattgcaaa
    30481 tctataaaaa tctggatatc ggtacagcaa aaatcaaaga agaagagatg aataatgttc
    30541 cgcatcattt gattgatgtg atcgaaccta ctcaacgtta ctcagccgct gattttcagc
    30601 attcaggtcg acaattgatt accgagattg cgaaccgagg gcacctacct atcatagctg
    30661 gtggtacggg tctctatatc caatcccttc tatatgatta tcagttagga gcgacagaag
    30721 aagcgaatcc tgagattaga aaaaagtatg aaacgttagc tgaaacaatt ggttcccaag
    30781 cattatggaa tcagttgaaa gaaaaagacc cggcagctgc tgagaaaatc cactggaaca
    30841 atcaacgcaa agtgataaga gctctggaag ttttagagac gacaggacat agtatcacgg
    30901 cacctaaaga agagccaaag caattgtatg attacctcat gattggatta gatacgaagc
    30961 gtagtattct ttatgaacga atcaatcaaa gagtcgattt gatgctagca gaaggtttag
    31021 tagcagaagc gcgtaaggtg tatgagcttg gagaagtcca ggccagtcaa ggtattggat
    31081 ataaagaatt tttcccttat tttgaaggga aagaaacatt ggaagaagca acagaacaag
    31141 tcaaactaca ttcgagaaga tacgcgaaac gtcagttgac gtggtttcgt aaccgtttgg
    31201 atgcacagtg gtttgatctt ataagtgagc caaatcaaaa aagtcagatt gagcaaacaa
    31261 tcaaaaaatg gctggaggcg gaataaaatg gaagaaaaag tgatcatcgt aggggtagaa
    31321 accgaagcaa accagcgtta cttttcagag tcaatggaag agctagttca gctgacgaat
    31381 accgcatcag gagaagtcgt attcacaatt acacaaaaga gaccacaagt ggatcggcaa
    31441 acgattatcg gtaaagggaa attacaagaa ttagttcaac aagcggatgc gcatgaagcg
    31501 gatttgatca tctttaatca tgagctaact ccgagacaga gccaactgat taccgatgct
    31561 gttggtcttc cggtaattga tcgcgtgcag cttatcttag atatttttgc gatgcgtgct
    31621 cgttcgaaag aagggaagct gcaggttgaa cttgctcagt tagaatacct gctgcctcgt
    31681 ttagtagggc aagggaaaac attatctcgt cttggcggtg ggatcggaac aagagggcca
    31741 ggtgaaacaa agttagaaac agaccgccga catatacgga ataaaatttt tgctgttaag
    31801 aaagaattga aagaagtaga agctcatcga gagcgcaatc gccaaaaaag aaaaaatagc
    31861 gagatttttc agattggact tattggctac acgaatgccg ggaagtcaac tatcttgaat
    31921 ctattgacac aagcggatac ttattcgaaa gatcaattgt ttgcaacgtt agatccattg
    31981 actaagagat ggcgttttgc tgaaggattt gagattactg taacggatac cgtcgggttc
    32041 atccaagacc tgcctacgca actgatcgat gctttccatt ctacattaga agaaagtcaa
    32101 aatatggatc ttttgctaca tgtagtcgat gctagttctc cagatcgtat tttacaggag
    32161 cagactgtct taaaattgat ggatgaactg aatatgaaag aaatgccgat actgactgtt
    32221 tacaataagg cagaccagat cgacccagcg atgttcacgc ctacgctatt cccaaatgta
    32281 ttgatttcta ctcagacgaa agaaggtaag atggcgttga tcgaagcaat caaacgtcag
    32341 ctgatggaac taatgacccc ttatacactt tctgtgccaa gcaacgaagg acagaaatta
    32401 agcgagttga gaagacaaac aatggttttg gacgaagcgt acatggaaga aacaaacgaa
    32461 tatgaagtta aagggtttgc aaaaaccaat tcaaaatggt taagaaaaaa ccaataaaat
    32521 tttatttttt taa
//
LOCUS       DANJMI010000033        30949 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000033 DANJMI010000000
VERSION     DANJMI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.033"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            46..1497
                     /locus_tag="PWU81_001790"
     CDS             46..1497
                     /locus_tag="PWU81_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL1110490.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1846..2478
                     /locus_tag="PWU81_001791"
     CDS             1846..2478
                     /locus_tag="PWU81_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HDL1110491.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3066..4505
                     /locus_tag="PWU81_001792"
     CDS             3066..4505
                     /locus_tag="PWU81_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1110492.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4800..5018
                     /locus_tag="PWU81_001793"
                     /pseudo
     CDS             4800..5018
                     /locus_tag="PWU81_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5267..5482
                     /locus_tag="PWU81_001794"
     CDS             5267..5482
                     /locus_tag="PWU81_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110493.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5699..5955)
                     /locus_tag="PWU81_001795"
                     /pseudo
     CDS             complement(5699..5955)
                     /locus_tag="PWU81_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6142..7053)
                     /locus_tag="PWU81_001796"
     CDS             complement(6142..7053)
                     /locus_tag="PWU81_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL1110494.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7072..8076)
                     /locus_tag="PWU81_001797"
     CDS             complement(7072..8076)
                     /locus_tag="PWU81_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDL1110495.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8073..10196)
                     /locus_tag="PWU81_001798"
     CDS             complement(8073..10196)
                     /locus_tag="PWU81_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL1110496.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10201..12648)
                     /locus_tag="PWU81_001799"
     CDS             complement(10201..12648)
                     /locus_tag="PWU81_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL1110497.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12635..13024)
                     /locus_tag="PWU81_001800"
     CDS             complement(12635..13024)
                     /locus_tag="PWU81_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL1110498.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13084..13587)
                     /locus_tag="PWU81_001801"
     CDS             complement(13084..13587)
                     /locus_tag="PWU81_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL1110499.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13600..13824)
                     /locus_tag="PWU81_001802"
     CDS             complement(13600..13824)
                     /locus_tag="PWU81_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110500.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(13928..15838)
                     /gene="ltrA"
                     /locus_tag="PWU81_001803"
     CDS             complement(13928..15838)
                     /gene="ltrA"
                     /locus_tag="PWU81_001803"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL1110501.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSETSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16584..16721)
                     /locus_tag="PWU81_001804"
     CDS             complement(16584..16721)
                     /locus_tag="PWU81_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL1110502.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(16718..17902)
                     /locus_tag="PWU81_001805"
     CDS             complement(16718..17902)
                     /locus_tag="PWU81_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL1110503.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(18161..18451)
                     /locus_tag="PWU81_001806"
     CDS             complement(18161..18451)
                     /locus_tag="PWU81_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110504.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18441..19583)
                     /gene="dcm"
                     /locus_tag="PWU81_001807"
                     /pseudo
     CDS             complement(18441..19583)
                     /gene="dcm"
                     /locus_tag="PWU81_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19659..19823)
                     /locus_tag="PWU81_001808"
     CDS             complement(19659..19823)
                     /locus_tag="PWU81_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110505.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(19816..20490)
                     /locus_tag="PWU81_001809"
     CDS             complement(19816..20490)
                     /locus_tag="PWU81_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL1110506.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20568..21908)
                     /locus_tag="PWU81_001810"
     CDS             complement(20568..21908)
                     /locus_tag="PWU81_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL1110507.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(21991..22326)
                     /locus_tag="PWU81_001811"
     CDS             complement(21991..22326)
                     /locus_tag="PWU81_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HDL1110508.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(22336..22710)
                     /locus_tag="PWU81_001812"
     CDS             complement(22336..22710)
                     /locus_tag="PWU81_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL1110509.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(22723..23037)
                     /locus_tag="PWU81_001813"
     CDS             complement(22723..23037)
                     /locus_tag="PWU81_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL1110510.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(23150..26377)
                     /gene="fss3"
                     /locus_tag="PWU81_001814"
     CDS             complement(23150..26377)
                     /gene="fss3"
                     /locus_tag="PWU81_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDL1110511.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27165..27488
                     /locus_tag="PWU81_001815"
     CDS             27165..27488
                     /locus_tag="PWU81_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110512.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            27652..28569
                     /locus_tag="PWU81_001816"
     CDS             27652..28569
                     /locus_tag="PWU81_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1110513.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            28562..29344
                     /locus_tag="PWU81_001817"
     CDS             28562..29344
                     /locus_tag="PWU81_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1110514.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            29345..30025
                     /locus_tag="PWU81_001818"
     CDS             29345..30025
                     /locus_tag="PWU81_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1110515.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            30018..30914
                     /locus_tag="PWU81_001819"
     CDS             30018..30914
                     /locus_tag="PWU81_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL1110516.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
BASE COUNT         9086 a         6299 c         5586 g         9978 t
ORIGIN      
        1 tttaatagaa aacactatta aagagaaaaa agagaggtaa atcaaatgaa aaaatcttta
       61 aaacttgcat cagcagttat attaatgtcc caactcctta tagcaacacc tttccatgtg
      121 ttggcaagcg aaaattcaac atacaaaatt gcaaaagaaa gaaccgagac gactgaaatg
      181 agtaattccg atgtaacagc agagagtagt gaacttttga caacttcatc aagtgaaagt
      241 agtacgactt caaatgccga gtcttcaaca acttcatcaa gtgaaagtag tacgacttca
      301 aatgccgagt cttcaacaac ttcatcaagt gaaagtagta cgacttcaaa tgccgagtct
      361 tcaacaactt cgtcaagtga aagtagtacg atttcaagta ccgaatcttc aacaacttca
      421 agtagtacaa gtgaaataac ttcttcgaaa gtgacaaaac caaaccatat agaatcttca
      481 aaaaaggaag cgccaaaaac ggaaggacaa acaaaaactt tttcgacacc acagcaaata
      541 gaatcaacag aatctttcca atcacaacca atactatctg gtaatgaaga aattcatttt
      601 gacaaaaatg aatctgtaga aaattttatt atgaaaattg gtgagtctgc gagaaaaatt
      661 ggtcagaaat atgatttata tgcttctgtt atgattgcac aagcgatctt agaatctgcc
      721 agtggtcaaa gccagttagc tcaggcaccg aattacaatt tatttgggat aaaaggaact
      781 tataatggaa actttgtcat tatggtaaca aacgaagatt tgggtaatgg aacgctttat
      841 acgacccaat caaaatttag agtgtatgaa aactatgagg aaagctttga agattatgcc
      901 aaattattaa ctaagggaat tagtggaaat aaagattttt atgcaggagc attaaaagct
      961 aatagtaaaa catatcgaga agcaactaaa ttccttacag gacggtatgc aacagataca
     1021 caatactatt tgaagctaaa tgaattaatt aaaacctacg atttaaccaa ctatgataaa
     1081 gaagttaaag gtccacaaat gagtaagact ggataccaag tgcctattca taattattca
     1141 ataaccagtt tgtttggaaa tcgtggaaat gaatttcacc gaggacttga tatggcagct
     1201 acacagggtg agccgattca tgcagttaaa gctggtaagg ttgttaaagc agagtttcac
     1261 tactcttggg gaaattacgt ggtgatacaa cacgaagatg gatcaaccgc gttatatgct
     1321 caccagcaac aatacctagt aaaaactgga gataatgttt tgcaaggaca aataattggc
     1381 tatgtaggaa gtactgggaa tagtactggc ccacaccttc atttggaaat ttgcaaagat
     1441 agtagtcttt ctcagagcat gttagttgat ccgaaaacct tattattcgg agactaaaaa
     1501 aggtaatcaa tctgacaaag taacaagaaa atggttggat tcttagacct ttgatcagga
     1561 tattgagact ggaattgata tctcgattac gatgggtttg acaaataaaa caagtccatt
     1621 atcgaatatc gagtgtatta ttgccgtctt tatgctcata ttttgagcag gtttcactag
     1681 atgaaaacaa tttatctatc ttgaggattt cgtgtacgta tcgttcagtc ttgtattgta
     1741 aattagtcat aaaatcagac taagaaaaat tggaaatatt tttttctgat ttattaaaag
     1801 ttaattgaaa ttatatggta cttctttctt gggagggtag gtgcagtgaa ggaaagatat
     1861 ggtcacctta ctgttgttga ggagattact cataatggat ataaaaaata tcgttgccta
     1921 tgtgactgtg ggaatgaaac gattgtatat cgatcaaact tactatcagg tagaaccaaa
     1981 agttgtggat gtggaaatta taaaaatagg cataaatata aaaatttgac aaataaaact
     2041 tttggtaagt tagttgcaat caagccaact aaactgcgaa gtgaaggaac tgttgtttgg
     2101 gaatgtcaat gtctgtgtgg gagaaaaaag ttagtatcgg caaagcgact tctaaaaggt
     2161 gatgtaaaaa gctgtggatg tttgaaatca aaaaagcatc acattgaaaa taaaaaattc
     2221 ggattactta ctgcaaagta tcctttggac aataactgtt catggaaaac aaaatgggtt
     2281 tgtcattgtg agtgtggcag tatgtgtgag ataagttata gtaacttatt ttttggacat
     2341 actcaaagtt gtggatgttt aaaaaggcaa gaaacaagga catgtgttga agggacagta
     2401 atagagtgtc taacgtcaaa attaccgaaa aataatacaa gtggtgttaa gggagtttat
     2461 cgttctaaag agaaatagat tgcgtatata actttaaaaa aaaagaggta ctatttagga
     2521 tcgtatgatt ctattaagag tgcagctcaa gctagaaaag atgctgaaaa taaattgttt
     2581 caacctttga ttgacaaata tcgaggtaat cattaattat tgtggataat aattaacagt
     2641 gatatataaa catctataat atattattca tactattgat atttagtata atcctatgga
     2701 attgaatatc atagcaatgt atttgaaaag caaattggaa aacaaatagg tgcaacacca
     2761 taaatatgaa aaaattggga aaaagtagaa tggaaatttg cttacgtctc acgagatagc
     2821 tcacactatt aatctgaaga acaatgaagt cgacttttag aaattgctag aagaaagtaa
     2881 agcgaattat cactagttac tatagagtaa gttcaaataa agcatctcca acttacatgg
     2941 ttacatgagg atatcaaatc gaaattactc aagcttccta tggctagagt cgcgagaagt
     3001 tgttgggcag tagacatagg ttatattgtt cttagctaca cgatcattag aaaggaaatc
     3061 ctattatgta tagttatcaa gaagatgcaa aagtccaaaa aaatcgctgg tggattttga
     3121 ttgctatatc tatgtttacc ttcatgtcca cattggatgg cagtattgtc aatattgcac
     3181 tacctaccat ctcagatgat atgggcatac caatgaatca agcggaatgg gtagtgtccg
     3241 tttatctgat gattgtctgt tcttgtttgt tgctgtttgg taaaattggc gatagccgtg
     3301 ggaaaatcaa agtatttcgg attggtacgc tgatctttgt cttgggatcg ctgttgtgtg
     3361 ggttcaatca ttcgctaggt attttattgt tcgcacgggt ggtacaagcc attggtgcta
     3421 gtatgactat ggctacaaat gccggaatca tcacggaagt ctttcccata catgaacgtg
     3481 gtcgggcatt gggagcaata ggtgcgtttg tttcattagg ctcgattgct ggaccaggtc
     3541 ttggcggctt gattttgtcc caattttctt gggcgtacat tttttggatc aatgtaccgg
     3601 tgggaatcgt aacgatgttg attgctgaaa aattcttacc aaaagatatc accatgagcg
     3661 gccatacaat cgatggaggg ggctttgctg ccattgcggc tacgattatc agcttttttg
     3721 gagcaatctt tttggggcaa gaaagtggct ttttccatcc agtccccctt gtactgttcg
     3781 taatttcagt gattgccttt gtcagctttt taaaagttga aaagaaagca gcctctccat
     3841 tgatttcttt cggtattttt aagaacaaga tttttaccat gagcttgatt acagccttgt
     3901 tgattttttc ttccaacttc tttgtcaatg tagtgattcc tttttatctg caaaatgctc
     3961 gcgggttgag tcctagctat gctgggctgt tgatgatggt ctttcctttg atgatggtcg
     4021 tcggggcgcc tttaagcggc tacctgaccg accgggttgg cccggagatt ttggtattag
     4081 taggcttggg cttattgtcg gtaacccagg tcatgtatat gtttttgtca gtggactctc
     4141 ctttgtggct atacattgtc gctactgcca tcatgggtgc cggcaatgcc ttgttccagt
     4201 cccccaataa tacgatggtt atgagctcgg tcactatcga aaacttggga ctggcaggct
     4261 cgatgaattc ctttgcccga aatttgggga tggtcagcgg gatttcgctt tctacgacgg
     4321 tcttatatca agcgatgtct gctaagatgg gccagcaagt aactaactat atcgcaaatc
     4381 ggccggatgt ctttttatat ggaatgaaga ttaccttttt aggcagcttt gttttgtgtt
     4441 ttgttgcctt tttcctgact atctggcgta tcaaaaaaca aggaggtatt cagaaaaatg
     4501 tctgaaaaaa ccactgttga agaatttttg gaggcctgcg tatgggtgag tgggatttcc
     4561 atggatgatg gaagatgaag tgaacttcta aaagccgaaa aactcaagta ttatcttcaa
     4621 aatactagtg ctcaatgaga tgcttgattt gatcacaaga agactgcgaa agccgctggc
     4681 aaccgcaagc taggtatccc accgatggac tacgttttag aggctactgg ttatgaatac
     4741 ggagccaacg ctctcatctg cataagaata catcacccag attatgtaaa gattttcgat
     4801 acttctgtca aaaaatcatt aagatagata aatgatttcc gtctagtcaa attggttcag
     4861 aatgtagaca cacagatggt aagaagtcac tcacaattcg agaatggact tgtcctattt
     4921 gccgtataca tcataatcga aacgtcaacg atagtatcaa tattttgacc aagggctatg
     4981 aatacaagca ttgccttaga aaacatcaag aaccttagga acaacgggga tagcttggtt
     5041 aataagagat accgctgttt aggcttgcaa gtatactctt ttatcaagaa ttccgtggct
     5101 ttagtcatga gaggcttaat actatgaaaa ttttctttag catttttgat ttaggttcct
     5161 tttctaatta taataagatg accactacag taacctactg aaatatataa acagccactg
     5221 tagtaccatt ttcaaagtag tctatgagag gagaatcttc attcttatga acgctggaat
     5281 tgcggatatg aatttaataa aaaaaaccct taatgatttt acttccaatt caatatcaaa
     5341 aggtactggt atcaatttaa gcacaataaa aaagctgaaa agtggtgaaa gatcagtgga
     5401 aaagctgaat cttttagatg ctataaaaat aacggaattt gctatgaaaa atggaaaagc
     5461 tgagattgaa atatggagat aattatcgtc tagtgaatta cttgccactt agctgaattt
     5521 aacgatcctt ggtgcttgaa gggggagatt cttgggaata gagcatactt gcaagcctat
     5581 gtctttacta acaacgatat ctcttattaa ccaagctatc cccgtagttc taaggctttt
     5641 acttttatct aagcaataat cgtattcaaa ggaaattttt gacccttaga aagtctcatt
     5701 ttatcagacg tgagcttagg cattgctgaa ttttgttaat tcggttttag gttgtgcttt
     5761 gagcataata tgaaagtatt ctttatcacg atttcattca tctaaggtaa tgtgatgtga
     5821 ttctgaaatt ctttcaaagg cagtttttgc ataagctgac attacatcat caatgacatg
     5881 tttacaatgt ttcactacta aaataagggg gtaaccaaga agaaaaactg aatgattatt
     5941 tgtatctaat tccatggtat atcaaccatt tcctatatag actgattatg acatggatta
     6001 aaataaaaag gcactatgtc ttaattgtcg gttttcgagt taccgaatgg cattcatcac
     6061 ccacctatag aggatgggcg acttctgcct gaaataagtt acactactta taacttctaa
     6121 taaaattaga ttgagtcatt attatttaac atcaacgatt ttccagtttc catttttttg
     6181 taatactaat ttgtactgaa agtagctagt ggtttttgat acattatcaa ggtattttat
     6241 ctttgtactc actagaaaat tttttccgtt ttgtagataa actggattct ctaactccga
     6301 aaactttaag tcaatattga ttggtcgcat cgcaccattt tcaacataat actccaattc
     6361 tttttcagaa gctgatggat aaagtttaaa aaatgtttct aaaaaatcgg tgatttcttc
     6421 cgttgttttg gcatcaattt ctgaatcgtt ttctacttgt tttgcttcat attttgcctt
     6481 gtctggcttt ccaatcattg ttggcaaact agttactaaa tgattcctgt tttcatcttc
     6541 aaagattgtc actcgatatg cagaagaaat tgattgcgtc ttttttccct ctgtaatatg
     6601 ttgctccact aaaaatgaaa cgacaaattc ttccgagtgt ttttcaacat ctagaatttt
     6661 tacagattga acttcggaac tcgtgggaat atctgctcga accatatctt ggattaaagc
     6721 gagaccgttt tctgtaaggt actgttcgag attactcatt ctcttttcaa ttgcttcttt
     6781 gttatttttc catgaaaaat attctttcgc aaaatcctct gtaaaacttt caacgccaga
     6841 agtattcact accttctctt ttataaccac tttttcatga gttgtatgtt gatcaatggc
     6901 ggtgaaattt ttataaacgc caaaagcaag actacaagac aataaaagcc aaagggcaag
     6961 caccattttt cgatgcttac ccacggatac aactcgttct tttttctttt ttgggctctt
     7021 ttccttttga ttccgttcaa tttttatctt cattcttttt cctcttttct cttatttgta
     7081 tcggccagct ccgataagat gctgccgcca atagttttcc gttagattcg tccaccccac
     7141 tggatctcct gcatgaaaca tgcgattctc ccctgcgtaa atgcccacat gggtcacata
     7201 agtaccggca tcataggttg aatgaaagaa aactaaatca cctggtttgg catcttttag
     7261 atcaatatga gtcatggcat catattgatc ctgagccacc cgaggaagtt tcaggtcaac
     7321 tttggcataa caccattggg tcaagccact acaatcaaac cccgtttctg gacttgagcc
     7381 accaaacaca taggccgttc cttcatactt aaatgcctca tcaaaaattt cttggactat
     7441 tttgtcatca aactgagttg tagttaggta ttgcttcact aataaacagt aaaacatatt
     7501 gccataatta taccgccacc caccattttc cttaatcgcg attgggttgg ggtaatcgac
     7561 tttttgacca cctgattttt cctttgaaaa agattcagct aaagcaaaac tatgcttctt
     7621 cccgtttttc ccgacatgat ctagataccc gccaccatag ttataggcct gaataatact
     7681 atcttgatca caccctagtt ctttggaacg cttaaccaaa gacgcaaaat agagacagcc
     7741 ttgtttgatg gatgcctcgg tacttaatga attaggtggt aatccggctg actcagaact
     7801 ttgcataacg tcttccattg tgccaccaga ttccacttga ataatcgcca aaagaattgg
     7861 aatatactct gttatatccg attctttggc atacttttcc accattgcac ggtgtttcaa
     7921 tacttcttct gagacactag ttccgccagg agaagtgcca gaactaccat ttccctcact
     7981 gtcttcacca aacaacaaac ccacacacat caaaataccc ataaaagaga tggtcagaaa
     8041 agttaatgaa ataacaccca ctttcttcaa gttcattttt tctcacctga ccttgatttt
     8101 ggacgatggt tcattctttt ttgtactttt tgattggtct gctttggcag agtggacctc
     8161 tgtttcactt gttggaaaga atttttctta cttgctgatt ggcttttccc ttttgaaatg
     8221 atttcttgac tagttagttt tcgtttgatt tcgggattca gatttgctct cacaatccgc
     8281 tttggtgctt cttttttcag catcatatct ttctgaggtt gagctgcttt atctattgga
     8341 atattcgccc ttctttttcc ctgacgattt gttgcttcac tcagtatctt gcgtttttcg
     8401 tccattttcg catttcttcg ttgtcgttgt ttttctcgca aatccactcg atccgctttt
     8461 tcttggaaca gtccgcgctt gaattcttct ggtgcttttt tcgacttttc gattccttta
     8521 tggagattgt atttcaaatt cgttggcgta ttccttacct gatcctttac ttgtttagtc
     8581 ttatctttca ccaaagcttg ggtatccaaa actttgccga tttttctccc agccaaattc
     8641 attcgggatt gtttcttatt atttgaaact ggattctcct tagttaagtc agatagtatt
     8701 tcgtgatcgt tcggtaatcg tgaattcttt cgtagagaag aaccggatgg cttaaatttg
     8761 gattttgatt gttcctttgc caacttattt ccgactgctg caccaccacc aaaagccaaa
     8821 gctcgtccca catttcgatt gagttgccgc aattttcgat tcattaacat ttgcggtttt
     8881 cgcatgacgc gtcttcccat tgactggcta tcattactct ggaggttgaa catactcata
     8941 atgtcgccca atttgaaata gataccggca aaggtcacaa tttgcagaaa agcaaccatg
     9001 aaaaatggat aggttgccga aatattgaaa aacatcgtgg agatgctaaa tgcagtcgta
     9061 atcaccaaag taacacccgc tcgcatcata atcgtattga ataaacgaat aatcgccttc
     9121 tttcctaaac tttcataagt cggcaacatg gacaataaga aactaattgg cagaaacatc
     9181 gcaaagataa tgaagagaat ttgcgagaac aacataatcc ctgttagaag aaaaacaaag
     9241 aaggaaatgc caatattgaa caataccaaa aagaccacgg tccctaaacg gttcatggtc
     9301 ttggtaatac tgagattcgc attgtcattg tcttcaatct ctgccttgac cgcttcttcc
     9361 cgatctttgc ctttgttttc atctggactc acagatagaa tcttgttgac ccggtcttca
     9421 ccaatttctt ctatgttgga atcatcaaat tgcagtagta accaaggttg ttggacttga
     9481 atcgaaaaca aactatcccg aatcaaatcc acactatctt ttccttggct ttctgaattg
     9541 ggtacaacga tctcggttcc aagggtaagg gccgcttcac taacatctga gctaaagtca
     9601 ttgatttttg tgatataggt aggcgcataa gcaatgaacg aacccgatag tagaaaaatc
     9661 atcacaaaat taatcacggc tcgaactgct ttcgtggttt ctcgttttaa taatccggta
     9721 tacgccacat acacccccag agctaaaatc atcaataata gaaacccaaa ataaaatccg
     9781 gaggtctgga gtccattttc agtaatacca gccaatgttt ggatattctt tccaatactt
     9841 tcagccgtat ccgaaatgaa atctaatttg taagcctctt gaaccacata acccgtggcg
     9901 ttcgacaaat acaaactcac cgtccaaatg aaattagtaa tggcataaag accatacatc
     9961 acgcttttcc ctaagccatc accccaattc caaggcaacc aatcccaaga gctatccaca
    10021 aagaaatcta gttgatagtt gttcagtgaa tatttggaat atagatttcc tgcttcaact
    10081 gtatcatcaa ctagccctgt cgcctcggct accgtaccaa ctaaactcaa tagaactatc
    10141 agagtgagta tgctaataaa aacgattccc acaatccgaa aaagtttctt tttcatcccc
    10201 tcactccatt ctctcttgaa caggtggacg ggtatcaaag gcatggaaga gttcttcaaa
    10261 gactggatgt acctgaacca cgcctacccg accatacaaa tcctgcatca agcattggcc
    10321 attttccaaa tctcgtaggc gtttttgatt gctttcatct tctcgatcca agccaaagaa
    10381 ttccaaggtt ttcttgattt catgaatatc ggtactgcga aacgcaaact tcaacccgat
    10441 attattcttc aagcgttcat ccagtaaatc atcactattt tgcgtgacaa agtaaacccc
    10501 ggcattcata gcccgcccag cacgaacgag cttattcgat aatgttttcc cttgcgctac
    10561 ttgaagaaaa ctccacgcct catctaagtc tacaattttg aagatttctc gattactatg
    10621 gataaagtcc aaggcaaaag tggaaatgac gattagcatg gaaacactaa gcaattccat
    10681 ggtggtatat tcttcaaaag tggtttctgc atctggtaaa accaaatccg ctacttgaat
    10741 gatatttaac tgcctatcta aactaatcga ctgggtaatc gaaccatctg agaaaagcaa
    10801 atgggcaaaa tcataatcgg taaaggactc gatgtgatcc gcgatatttt ccgcaactgg
    10861 tgagccttct tttcgaagct cattaattac ttgtaataat ccccgttgat tgctttgggt
    10921 aaccgtccga atggccttac gcaacactgg aaacttctcc ccatctcgac tagaaattcc
    10981 tgttaaaaag gtcagaatat caattgctag actttcagaa tccttctttt tctccataat
    11041 cacataagga tcaagtaatc cttgattttt tggcttacta gttaagttga gaatattgat
    11101 ttcatccgca atttctggca aggtttcttt ccagccaccc cgttcccctt ttggatcgac
    11161 aatcacagct tgtccaccaa aaaggacggc gtaataaact aagagattgt tactaaagga
    11221 ctttccgcca ccgagtgaac cgaggaatgc cgctgctagc gcattggtaa ctgaatcctt
    11281 gaccccttgt gaggccaatg ccggtttcag gtaaacattg cgccccgtat ctacgttata
    11341 accaaagtaa atgccttcca actcccctaa catctgagtg gcgccaaaac caagtccggc
    11401 gagaaaatca gacgtaacat actggatata atcattcatg tatcgcttac tagaaggtaa
    11461 gaattcttca tgtagcccca tcatatcgcc aaaaggccgg actagtttta cattggtatc
    11521 atcataaaaa tctagcactt catcacaccg gcgctttagt tcatctactg attcggcact
    11581 tactcgaacc acatagctca gtttgtacat ggattctttg gactgatcca aggtagtttc
    11641 caattcatct acggaatcca atgcatctaa gacattggaa tttgtttcat tgtcagattg
    11701 ataggcatgg ttgtccaagt cttttaattc ctttttctta ttgcgaacgg tagcaagtgc
    11761 ctttttgttc gtcacaatct ccacattcat actagtgtca attgggaatg taaattgttg
    11821 ttgctggtaa tagaaaagtt ctgaggaggg aaactccatt tcccccacaa ttgtattaat
    11881 tgttaagtac gctacatatg attcatgatt ctcatgttcc atgcataagt accggggctt
    11941 ttcttcaatc aaacaacgac tgggacgtaa taagtcgtaa cgtttcacca aagtttccga
    12001 cttcagcttt ttctttggta gctgaaattc atattcttca aatggtgtac ccttttcgcc
    12061 atagatatgt tcaatcaaat acatcagatc actcggtgtg actcgtctga ctttaaatct
    12121 tcgagccaat ttactttcca ttagtttttc aagcttggta tatcggcgaa tttcttcatt
    12181 ggataaggaa acaaaatcgc ccatcaaatg atgattcact ccatagacaa attcttgaaa
    12241 gccggagaag aaagatttct tgagattctt tagattgacc tcttcatctg tcgcaatcag
    12301 tttgaagcca ataaaaaagc gataatcaat ctgactatcg ccaatcatgg atactaatgc
    12361 ctctgtctgt aaatcaattc gttgtttcgc tacttctgtt aatcgcccgg taatttcctt
    12421 ttttgatcgt tcttgcgtgg cacgaatact actttctgtg gcaatttgta aggcatgaat
    12481 cttgccttca cgattttgcg caatcaattg acgaaagtta tcgtggactt gatatttctg
    12541 ttcaggtgac aaaaaggagt aattgtaagg tattaactca tagtaggcaa aacattctcc
    12601 ctcttgatta aagacaagat tattttcaat atacttaatc gggtacatag ttcacactcc
    12661 ttaccagagt aatgggttcg tcccatcgtt tcttctgttc tttgactttc tttcctgcga
    12721 aagtgacctt aaatcgtaag tgatagctaa taaaagatcg taaaaatccc aatggtttct
    12781 taccgtcaaa cgttttttgc gacatgaacc aagtaactgc caccggtata cctaagtatt
    12841 ttaaaaaggc cccctctatc atagatagtg gaggcacatg ggcaaatacg atgacaaaca
    12901 gcaaggataa aacaaaccaa gccatctggt taaaagtcac aggaaaaggc agttgaaagt
    12961 cattaattgc ataaatgacc ttttcaactg cccaaatact ggtgtagctt tttattttct
    13021 tcatcaaatc attcctttaa aaagaaggat agtttagaaa tcaactaccc ttcaataatt
    13081 ttgttaatat ggatactcaa aaacacctcg actcgttacc aaataattgc ctccaatctc
    13141 caagtctcta ccacacgcat caaaatcaat atacgtcttc aagtgttctg gtacttctcc
    13201 aaaatcacca gcttctacaa tcaagaaata tgctacatca ctcatatcat cgcaatctgg
    13261 ataatagata atatcatcca catgctctac catctcctca aaagaattga agaaagcatg
    13321 ttgaatctca gaagcaactt caccaactgg cgttccttca agttcctccg ctaagccaca
    13381 caaccgatta atttcctcaa ttggcgtata ctcatcaatc tcaaagggaa gttcataatc
    13441 atgaattgca tattcctcgt attcgtcatt taacccaatt cgttctttca cttcatcaaa
    13501 atcgacaggt ggggtaaacc acgcccccac caattcacct tcgttatatt tcccgaggtt
    13561 cgcaatataa acacgcatct gttccatcgt gcttccccct tacgcaccga caattcggtt
    13621 aaacaaactg agtaagacat ccttcacccc agaagcatta aagaccaatc cgaccgcaat
    13681 caaagcaatc accaaaaagc caatcagttt ggaaaactcc cgcttaaatc ctaaaaacaa
    13741 accaatgacc acaattgcca gtaagactaa ggattgggca ttggttaaaa accattgata
    13801 tatggatagg taagttcctg atattataaa tatctcaaac ttttcccccc attcacacgg
    13861 agcgtgcgac tttcaccgca ctccgcgttc catcgtgttt atattgaaaa gtatttatgt
    13921 agtagaccta tatcaagttt aattcttgat tgccagctaa tactctatat ttatttagtc
    13981 ttatgagctg gactttattt agcttcaata gagctatata tttttgtatt gtttcttctg
    14041 ttttagcgtg tattagacgg tgtaccgcct cttttaccaa aataagattt ttataatcgt
    14101 catttccacc agctccagtt ggtacaatat gatgacaatg tatttcatta gcttgttgaa
    14161 attcttccag agttatagca cattttccat gttgagcaga aaatagcgaa attcgattgt
    14221 ctgcgtattc tattgtgcga ttttccaaag ataatttcat taactggtac atcagtaatt
    14281 tatcaacact tagattggtg tggataaatt ttcttccctc tagagtgtag caattcgcta
    14341 aatacacctt tgccattggt tttctgtgtt ggatatatcc aacagggaaa ataggttcgc
    14401 cctcgccagc aacataccga agcattttag atttaccata tcttgttgcc tcctttgtcg
    14461 taagttgtct gcctgttttt ctcaatctgc cccttttgtt tgtacctaat ctgttagtga
    14521 agatagccat tacagctcta ttcagtttca tacaatccag atttacattc gttgcaatct
    14581 gataataatt ttgcatacct aataccattg aattataaag tcttgtttct tctaattccg
    14641 ttttcccttt tgagggagaa gcaattttct ttgcctgtga taccagcttg tctttttggt
    14701 ttttgaattg tttatcactt atatgtgatt taacaactaa cttctttctt ctcggtatca
    14761 ttttaatctt gaaaccaaga aagtcggagt atctttttct aacatttaca attcttgttt
    14821 tttcttgtga tacttccaat tttaatcgtt gttcaatcca ttgagtgatt gctattttgg
    14881 ctttttcagc actttccttg tagcggcaga aaactctaaa atcatctgca taacgaacca
    14941 tatacatttc ctttaacttt gttttcttca tttctttgta agcgttgctt ttacatgggc
    15001 taccgctttt attgatatga accacatagt tttttgtaac aggattttcc tgccattggc
    15061 tttctaccca atggtctaac tcattaagaa caatgtttgc tagtagtggg gatattattc
    15121 ccccttgcgg agttcctttc tctggatatg ttattgtacc gttagtcatt ttgattggag
    15181 cttttaatat cctttttaaa atgaatatca agtgcttgtc ccgtattccc atagcccata
    15241 tctgttttat cagcttggaa tggttcacgt tgtcaaagaa acccttaata tcaaattcaa
    15301 tcacataatg tagctggctg atttgtaggc ggttataggt tgcttttatt gcattttcaa
    15361 ctgacctatt aggtctaaaa ccatagctgg tttcactaaa tttagcttcg cagattggtt
    15421 ctaatacttg ttttatacat tgttgtataa gcctgtccca catacaagga atacctagag
    15481 gtctggtttt accatttggt tttggtattt cttttcgcct cactggctta ggtcgatacc
    15541 catgtggact gcctgcaaca atgtatctta cttttttgac aactgtttct gctggcaatt
    15601 tgccaatatc tttaattatt gtaccgtctg ttcctgctgt tttactgcct gcatttgctt
    15661 tgatatttcg atatgccagt aatatatttt ctctcgatag gataatatcc ataagatgag
    15721 tgaaaatatc tccattctta cttttggcat ataaatcatc aaaggtgttt tgcatttcgt
    15781 aatattcagc gtgtcttaaa tcttctacac acaatgtttc atttttattt ttaggcataa
    15841 ggcttcactc ctttacttga aattgagtga gcttttttat cttactcgta atccttattg
    15901 aaacaacata atacttttct aatatataca cgacttgagg gcattcctcc attctcatta
    15961 caagaatttc ttaggttcgc cctctgcttt tcagcattag ttcaactgct tattgttctt
    16021 cgtcagttta ttctcttgat gagaacccaa tacctttcct cgttccgata atcttatcta
    16081 tacatatata tccttaggtc actagctata agcctgctgg cttgataccg cctgtaacgt
    16141 tatatggcat ttcataacct atattcttac tttgccacac cagctacgat ttctcgtacc
    16201 tttgcatttc tacaaagttc aaatttagac ccgtacattc gctgtttcgt cagttcctta
    16261 tagaacattc taaccataga tattttatag acccccgacc tgttggatac gctacccacc
    16321 tctggatagt tttcgataga agatatatgt tcatcaacta ttacggtatc tttcagccgt
    16381 ctttaccgag cttcaaacat tctgagtttc ctcatacaat gcttgtcgga ggctcagggc
    16441 aggcgtttca gagcgttact tcatcattcc acctgtcaga ttatcagttc tcctaataac
    16501 caaacttttt cgttctttta ttagtgcctt agcttttcac taaggaacga gtcgcacata
    16561 aattttgtcc gaaattcatc ttgtcacttt cctttctttt ccattgctcg atccgctgct
    16621 gcacttgcat tcatcaagca catcacagta acaccaagca tactgccacc aaataccaaa
    16681 aaaatatctt ttacaattcc catgacgatt cctcttttca tataatcaaa ttttcaattg
    16741 ccatactttg ttgctccaaa atctttttat gtcggtctga taagcgtgca ttctgtacca
    16801 tatcctggat ataggttgtc cgattcaatc gatccaactc cttagccatt ttccacgtag
    16861 gggcgacttg tcggcctagc caattcaagg ttcgttcaat ggtatacggt tttggctcgg
    16921 aagttaattg aatctctttc cgattccgtc caataaaacg cccccattgt tcattcaagg
    16981 gccagttggt tcgcctttta ccttctactt tatccgcaaa tcgcaagtaa cgattgataa
    17041 tggaaaacgt ggtgctctca atgctacgac cttttaacaa atcttgaatc gcatgaaaag
    17101 cacggtcatt tttcagtcga atctcaaaac gatttttcgt ctcagtttgg tctaatggaa
    17161 tccctaactt gatatattgt tcatagtctt tttcatacaa acagaaataa acctcactct
    17221 tgagactgcc gatatataag gtattgccca tgccgtcctt ttcatcagcc tttaaaagtt
    17281 ccccggaacg ataactttta aaggtacgaa acaaggagat acattcttcc ttctgacatt
    17341 ttttcgttag ctcaggtata tccaatagcc ccactcgatc attgattgcc aagtccaagc
    17401 gtttcatcac gccaccggcc tttagacaat cctcgaaaaa gtcgtaccag ctacgctttt
    17461 gagcgagtaa aaaagtttca aattgtcggc aacctcgccc ctttaattct aaaagcacac
    17521 ctttatcttc ttcatgagac aacatcacca caatatcacc catgacatac tgttcttgat
    17581 aggagtaaaa ggcataatct tcatggagca tatactcaaa tttcaaatgg agaatttctt
    17641 gaataatttt tcgtgcatcc gttgtgggaa agcgaatcct tacatagtca aacagtaacg
    17701 tcaaaggcaa atcgggatta aagctttcca gttggtcaaa aattttcctc atggttttta
    17761 tagatgcggt tacgccacca ttttctatct gatttaagta cggccgacta agtccacaag
    17821 ccgtagccaa tttattttgt gaaacgccgt attcgttgcg tcggtctttt aatagccgat
    17881 aatctaaatt ccgttgtgcc aagttctcac ctccaaattc aaggtgttaa ctttttcaaa
    17941 tgagttaaca cctatacaaa ttacttacta tctttgatac aacaagcatt cttaatggtt
    18001 gcttacttat tttttgtgtt tttgtacccc cctgttagat acgggggtga gacatcatgg
    18061 tcggctaacg ccgtccatga acaggcacag caccacggag gatttctgct tcgcgaatcc
    18121 tccgcggtca gcgcctaaca gtcagttgtg ttggtttagg ttactcatct gtaataccat
    18181 ttacttccaa ctctgctttc atttctaaca agtattgacg aatatctggt tcttttgctt
    18241 ggtccgcaat ccgctcaaat tctgcaatta cttgatttat ttcaattcga gcatccgtct
    18301 cctcattcat taaatatgga tcgccagcga ctttgacacc attcttgcga atatcaaatt
    18361 ttaaccgtcg caattgttct tctaattctg ggtctgactg aatctttcca atcacccaat
    18421 aaatttctcc aatcaactca tcataaacca tgtacatcca actcttttaa tcttctggca
    18481 atatcaaaaa tgaccggaac tgttacagag tttccggctt gcttatacaa ctgactatct
    18541 gaattgactt gagaatctcg atcaaaagcc caatcgggaa agccctgaag tcgccaacac
    18601 tctctaggtg ttagcttccg aatttgatac tgatccgtga cgatcgcttg ttggtctcct
    18661 gttagtaagg tatgaacact ctgttttccc agacgacctc ggcgacttgt ggaattagga
    18721 aaagccagat tcacactgtc ttctggcttc acctcttgat attctgtttc attttttcga
    18781 atcatgattt ttgtaccacc ttgtttcgtt gacaaggtgg gggcggttcc agtaacttca
    18841 taaactcgat taccttgttc aaactttccc ggtaatagtc ctgctacaga gattccatcc
    18901 cttgctggca gtgcgatttt gattccttca tcacctgtta aggttggcgc tacgccatct
    18961 gaaccataga cttgtccacc cattcctcga ttggaaggat tgatatttac caaatttctg
    19021 atgttttgat cagatgttga agacggtcca atgataggaa atacttgttc ggtacatgcg
    19081 tctcgagaat gtccgataag gaatatccgc tcccggcttt ggggaatgta ggcctttgag
    19141 ttaagcacct accactccac gtcatacccc aatccatcca gcgttcggag gatcgtctcg
    19201 aacgtagccc ctccttcgtg attgagcaat cctcggacgt tctcaaggaa taaaagctta
    19261 ggtcggagaa gagtggtgaa ccgagcgatt tcaaagaata aagtacctcg agtatcggaa
    19321 aatctttgcc gctttcccgc aattgaaaaa gcttggcacg gaaatccgcc gcaaaggatg
    19381 tccactggtc cgagggattg aataaactca tctgatacac ttgtgatgtc atgcatttcc
    19441 acctccttac ttgtatcatg aattgcttta taactacgtc tagcaaattt atcaatttca
    19501 caaaaaccga tacaatgatg gctcgcctgt tccataccta aacgaaagcc accaataccg
    19561 gcgaataaat ctaaaaatct catacattac ctcattcatt gcaaagaaaa aagccaagaa
    19621 cattttgctc ttggctcata cattattaat ttgtcctact attttttatt taatccaact
    19681 aatttccgtt gtttttcatt ggcctgatcg gctacatatt taatcaccgt tttgaactct
    19741 tttgcagtag cattttccga taaatccgca atgctcttat tgtatttctt tacaattttc
    19801 gttacttgtt ctttctcagt catgtaattg aacctccaaa tctttcctac tataccctag
    19861 tggtaaaaat aattctagat tatccgtagc ataatgaact tcggaaatat tattcgtata
    19921 atcttttatt atgatatgaa gcaatacatc tggatgttta ctctttactt ttgcaatgtt
    19981 ctctcttgat ttgaaaaaag cgttaataaa tgcctttttc ggcacatgac gtccctctgc
    20041 aatttcacgt tcttgtgtaa atttccaagc gattttaggg tcttgataaa catagaaaat
    20101 cgtcactcga aaatgttttt tcaacgcccg cgagatattt ttttcagcac ttaatattgc
    20161 aaaagttgca tccagaataa aagaatagcc atgatctaag acatatctaa atgcctgctc
    20221 taccaaccaa gaagatgctt tttgaaagag actagagttt tttccattat aatcaggaaa
    20281 ctgggtgcga aagtaatccg catcaataat cacatggtta tcataattct cagctaatcc
    20341 aatggcaacc tctgattttc cagccccagg actccctgcc ataaaaactg cgtctcgtcc
    20401 aacttcgggg gatttccctt tgataatata agaaataaac ttatccttat ttgcttttgc
    20461 atactctaaa taagaatcat tactttccat attaattatc ccctttcttc ctactatata
    20521 aaagtattat acgttacttg aagtataaag tcagtattga agatcactca atcattcatc
    20581 ttttctaatt tactaattga atctaagaaa tcataccctt ttggtacaag tggcgtataa
    20641 aactcactaa tcacactgcc acctgtatcc acataacctc gacctttgat gtgcttcata
    20701 aagaagtttt tttcgacctc cccaaacatc atggaatatc cgagttcact catacgaccc
    20761 aaagccacgc gaaagttgaa ttgatcgcgg attccgtctc ctaaatattt cgcatccggc
    20821 ctttggcaag ccaaaatcag aaagaaacca gattgacgac ccaacatcac gatttgtttg
    20881 agcttattca aaatcaccgc actttctttc gtcgttaaca tctccatata agccacgtat
    20941 tcatcaaaga ttaaaaagtt gggtggaagt ccaagatagg cataattctc tcctgttttg
    21001 tagttagaca tttctttcat tgccttgcta cgagccatca tacgttcata gaaatcttcc
    21061 acacaagcag aaatttcttc cttttgtgaa taaacatgag gcatcaccgt acctaaatca
    21121 gctaaatctg catttttggg atcgaggata aacaattctg catctgactt caatagtgct
    21181 tcgataatgg tgaggaggaa ataggtcttc cctccacccg tgccaccagc gattaacata
    21241 tggggtaaag aatcataagc ccatacttga tttttcatca atcgcagagt cccattttct
    21301 gccactactt cgtctatccc aattcgattg gcgatcatat catacagtaa agtatactcc
    21361 acataggagt ctttgagttc cttttctact aactcacaat acagtccgct ttctaacttc
    21421 ttttccaatt ttaaaagctg atcttggtat ttcccaagtg aaatttgaac gcgaatagac
    21481 agtaatccat ctttcattcg ataacagatt ttggggaagt aactgatagt ctctctggtt
    21541 cggctactat tcaaatcttt gaaaaagcct tcactttgag tttgtttcac ttcataccaa
    21601 tggttctcta gaatcatccg agctagcttt tgtcgatgcc acaagcttcg ccaatgatca
    21661 agaaaaaagt gaatataaag caatatcata gctcctgaaa aagcgagact tccaagcaga
    21721 catactaaat cttttataga ccaaacgatt tcaatagtag aaagtgatag cttagaaata
    21781 tcaaattgtc taaactcttg aaaataaaag acgctcatcc aaccgatgaa cagagcgagt
    21841 agccagccaa gaacaaagtg ataaaccaag tgctgatctc ctgcgcgtat gcgatgtcct
    21901 ttgtacattt tgatggtttc cacctccaat cgaaaaagcc caataggatt tcattaggca
    21961 atttcaaaaa gttatgctag tcatttactg ctacgaggag gggagttgtt tcgtttcaac
    22021 caattgattt aaaaagcctt caatagcatc cggcatctcc acaccaattt cttccaaaat
    22081 ggattctgcc tgccgacata agttttcatt tacttcgaca actataaaga aagactcttc
    22141 ttctcctaac cagtctagta catcagagtc ttcttcactg tcgctatttt ctccaagtaa
    22201 accttgatgt ataatcagaa acattttttc atcagaggtt aatgctttaa cgagttgttc
    22261 ctcagtcagc tcgccttttc catattgatc gagacaatct aaaatatgtt caacattaat
    22321 ttccataatc ttcacctatt tctttctttc attttggttc gtaggatttc cttgattcgg
    22381 ctgccgcttc aagactaaat catccgcctt aatgaaccaa ttaacggtag ttccttctcg
    22441 aaaaacataa ttggcaacgg tatccacgat tggattgacc aactctacct ctgcatcata
    22501 agcaaactcg cgtaagggta cattagctgg gatactaact tgaatcattc gcccttgtgc
    22561 agaggatttt aaattatagg ttcgttcttt tacctcatta ccaattgttc catcctcatt
    22621 ttgcttccgt acttctcggc gtaatgcgga aaaacgcaac ttcccaaatg ttaaacctga
    22681 atctacgaca ataccttctg ttaatctcat cttcttttcc tcctatgctt tcactaaatc
    22741 atcaacatac aacacgtaat cagtaaaccc acgattctca atctcatagc ctttggcaac
    22801 caaatgggga ttcacggctt ttagcggttg gtcttgatca aaatcctttt caccggcttc
    22861 cgcagctact accacctcaa tatcatcggc ccgttgctta ctggaataca aatgataact
    22921 gcgaccaatc accgtagtat tccgtccata tccttctgtt ataatttctc cttcaccggc
    22981 ataacttaac tttccaaagg tttcagccat atctggcacg acaaatttca attccatctt
    23041 cttttcctct tttcattgtt agttttggat acaaaaatag cgcgactact tttttaggag
    23101 gcggtcgcgc acgttggttc ttaggattta cttgtatcat ctcatcacct tagttccggc
    23161 gattccagaa ataatagccc gccgtcgcaa aaatcaaagt aaaaccaatg aacagtaaaa
    23221 cattggaatt tttctccccg gtttgaggga aggtcttcac tgtgggacta gattcttgag
    23281 agccacttgg aacagttggt gtttccggtt gtttcggcgt acttagtatg gttggaactt
    23341 cttttggtgt taaggtttga gatttttctt tcaaatcttc attgtgtttt ccattgatat
    23401 tcccatcttt atcgtaattg atttccgcaa aagtaaactt ggttccttct ggatattttc
    23461 cggtatctac ttttttcgca agcacagttt tggtgaattc tttatcattt acttcataat
    23521 caatcttgcc agatttccaa atttctttgt ttgtaccatc cggtaataaa gcatacagaa
    23581 ttgtttcgaa agcttctttt gagccatcca gtacatcatg agtaatcgac acatcatcaa
    23641 acatatccac cacatcacca tgagtaaagg tctgagaacc gttttctagg tgggcctttg
    23701 tttggataga aacatggtgt tccactgtac aactgacagt ttgggcttgg ttattcaagc
    23761 taacatcctt cgctactggc ttatcgcctg cttcaaaagc ttccttattt tcatacacat
    23821 agttgaataa gacaatagtt ttatttaaaa cttgttcggc ggttaattta tgctgaattt
    23881 tccaagttcc tgttttctct ttgttcgcca acgttgcgct tgtttctgca atcaccactg
    23941 gtttggcttt ttcatctttt tcttgggcag cttttgtcgc atccacatca atggcttgcg
    24001 ctaccacata ccagtcttct ttgatttcat gtagattgta actcaattta tcaactagct
    24061 cggtagaatc cagagaagtt aattctttgt ttccgtcttt ccaagtcgca agagaagtta
    24121 aactatcttc ttcctcaggc aaatcataga gcattaaacg gttcaaacta acagaaaatg
    24181 ctttgttcgt cagcttctcg taaggaacgg tcaccgtttt aattggttgt ttttggtctt
    24241 gttcggtaat ggtaaagaca tactcactct tcacaaagtc ttctttgttt tccttaaaag
    24301 tagaacggat ttctaatggc gtaatctttt gaaaaccttc tggggcttct acttcttcaa
    24361 gtaaataatc cccataagga agattttcaa acttgccata accatcaaac cctaattgct
    24421 cgttataagc tgtggtcgct tcatcttcgg cacctgtgat ttcattggtc ccttccaatg
    24481 gcgacacttt aaaggtcaga tcattgaagc cagtttcggt agtgcccgca gctgatccag
    24541 caaatttaaa gaaatcaaag ccaaagcgaa tcacttgttc ttttgccgta acatttcggg
    24601 taatcacggc atttttttcg ctatcatcca cctttttgat ggatacagga tactttgttt
    24661 catcaaggct atatccttcg ggcgcttttg tttcttgcca ataatactcg ttgatagcta
    24721 aatgtttaac ggcaacttgg ttcttttcgt ctaaagtcaa agtgaccgtt tcatcagaac
    24781 ctttcgttcc cttcgctaac tctggtttaa aagcctcact ccatttaaca gcttttccat
    24841 ccttagcagt aaacaaggtg tattcagcac cctcaaagac tgccttacct tgtgctttat
    24901 ccccagtatc tttgtcttct ttcgtcaaag tggtttcgcc agtgacttct tgattttgcc
    24961 cttttacgtt actggtaaca ggggctacgg tttgattggc atacttcaat tcgactttta
    25021 caggtttgaa cgtattcacg aacccatgac tagctttagt ttcggtcaca taataggttc
    25081 ccaattccaa cgcattagca atctcttttg gtgtttccgc atgaccgttt tcatccgtgg
    25141 tcatttcttg aacgatttca ccggttggac tgtctttgcg aatggcaaac gtattaccag
    25201 ctaaggaata attgtcattc caaagatcac tccctgtttc aactccgctt ttatccagaa
    25261 tgatttgccc tttttcccgt gcgttttttg aagtcacgta aatggtttcg cctgctttaa
    25321 tggtggtagt catgggggtg gtatcaatcg tataaggcgc cggtactgat ttttcggtaa
    25381 tggttacttt tgtaccatga ggaatctcat ccaatgtagc aatgccttct ttgtcagtag
    25441 tcacgtcttt tgcaggtaaa gtttttccaa agtctaaatg gaaaaccgtt cctggtacaa
    25501 catcacccga ttctttatcg acttttttga tctttaaaga accctccgtt tccacatcaa
    25561 ttttgattgt gtaggtatta ggtttatcaa tcgccccggc cattaaagtc tgttggccag
    25621 cctttttata ggcgacaggt gttccggtac cagcagattt cttaagcgtc agcacaccac
    25681 tttcttttga attggcattt ggggtgatta tcaactgatt accattcaca cgataatcaa
    25741 tgtttgcggc attttccaca acctcatcga actctgacaa attcagatta ttcgtatccg
    25801 tcacagtagt cgattgccct aatactgttt tagccgtact attatggaag gttggtttct
    25861 tctgataatc gtcaatggct tgatttattt ttgcctcaat ggctgcgata tttacggcac
    25921 taccatctga tcgttttatt gagtgaaggg tatacccatt aacttcttgc cagatcatct
    25981 tctgtgccac aatatgagta tctgcatctg tacctacttt tttccataaa acggaaacta
    26041 atttggcttt atcggacata tctggtaacg ggtttttctc atagccagga gtgaactcat
    26101 tgtaaatcgg gactcctggt tcaatacaga aaatatcttg tttaaagccg ttgtacactg
    26161 cataatgcgg aacagtgact tctgacattg gacgaccatc actaaaagtt ccttcaacag
    26221 catattgatg tgctaaatct agttctacgg ttaccgtttg tggatgggta atttcatcag
    26281 caaaagtaat cgtaggagca attgctccaa caattgcttg aacagccaaa ctcaaaatag
    26341 ttaatacaca taatttcttt ttttgttttt tatacatgaa atgttctcct ttcaaattaa
    26401 gcacaaaaaa ataagatgct cgcttgaaca tctttgttta taaagtcttt ttaaaatttt
    26461 tattgactga atagaatgga tttgagaatg aatgagccta aaaatttaat ctaaaatacg
    26521 aaatatactg cctttaaatt agaacacatt ttagaacaca aacatttcaa atttaatcaa
    26581 atataataaa atatatttac gggaaaacct ttataaatca aggtttttca aacttattca
    26641 atgatacgaa gaaaaaacca ggtcttaaga aagcccgtaa agcttcacag ttctcaaaac
    26701 gttaattgcg attatcgttc gagttcacga gttacgagaa tcgtactggg cgaagcaatg
    26761 ctgagcgcag ttaccttaat cctaactttt ctggcacttt ccacaatttg gaaagtgcct
    26821 tttttgtgtt tttagctatg ttaataggta ggattcttgg tctataatat cattcgatct
    26881 ataaacaaac gggaaaaaga taaagtatca tcaaacaata ttatttatcc gcttctgagg
    26941 ttgtgacaga agtggtcagc ttcaagaaat aagaaggaat ttacaaaaat tgcttctcat
    27001 gtttgataaa tattgtgact tgtgaccgag gagttggcag gagaacaccg tttattcgga
    27061 agaagggatt gtgaaaagtc ttttgtcata acctcttatt tggtagctaa ttgtaaatcc
    27121 caaacggcat gtattatttt tcataagtcc gtttttaagg attaatgttt tcttttgctt
    27181 tttatatggt ctggcttcag cgagaagttc caacgcatat acaaggacga cttatgtcaa
    27241 cgataacaac gattaccatg gtggccagtc cacttggatt agcactgttt ggtgcgctct
    27301 ttgattggaa gacaacgcaa ccacgtctgc gagacagtct aatttttctg ggagggagtc
    27361 ttgctatagt tatccttata accggtctgg taaaattact caagcttgat ctgaaagaag
    27421 caaaaatttt cagtgaagag gaatacagag aaacgacttt ggtcgaaaag aaagaatcag
    27481 gaaactgata caagcgtata acaaactgcc tgataatagg aaagacacgt attgtccgat
    27541 tgtttttttt gacattcaat tggaaaattt acactttctt tatacatttc tggctgcttt
    27601 ctttataccc gtcaactata cttcactcat caataagaaa gaaggataag tatggatttt
    27661 ggaaatgaaa cggtcttgaa agcaactggt ataaccaaaa aatacggtgc tgccaaagca
    27721 ttagataaag tatcaatcga aatcaaacgt ggaatgatct atggattgat tggggaaaat
    27781 ggagcgggta agtctacctt tatgagaact attatgggtt tgatctcgat tgatgaaggt
    27841 tcgatcgaat tatttggaac gacggatttg caggcagcaa gacgacgtat gggtcaatcg
    27901 attgaaaccc ctgctcttta tccagaatta actgcaagag ataacctacg tatccaagca
    27961 gcgaacggcg gtgtgtcgga tagagaaatc gaagacctgc tgaaaatgat gcgattggaa
    28021 aatacaggga aaaagaaagc aaagaatttc tctttaggta tgcgccagcg tttagctatt
    28081 gcaaatgcac tgatcacgaa tccagaattt ttgattttgg atgaaccgac gaatgggatg
    28141 gatccagcag ggatggcaga aatgcgagaa atcatccaac gtttggtaaa agaacggggc
    28201 atcaccgtct tgctttctag ccacttgcta gatgaattgt cacagatcgc cactcactat
    28261 ggtattttgc atgaaggaca cttgatcaaa gaattatcga aagaagaatt ggcacaagaa
    28321 tctcgtcaat ttataaaaat tgacacttcg gcgacggaac aagcagtaac ggttctcgac
    28381 tctcttggtt atagggatta ttttgtacaa tctagccgtg ttatccagct ttttgaagga
    28441 attgaccaag tagcagccat caatcaagca ttggtagaag caaaagttcc tgtggatggt
    28501 attcacctag ttgggcaaaa actggaagat tatttcttac aattaactgg aggcaatcca
    28561 catgtttaat ctattaactg ctgaaaaaat aaaagtatgg aagagccgta aaatgtgggt
    28621 ctcactagga ctgatggtcg tacttccgct tttttattct tggaacgaat ggtatatgca
    28681 tacaaaatac ggcaatgaat taaaccaagc aacggataca gtcatcaacg gtgcaacagg
    28741 gattttaatg gtagaaaaaa tggctgcatg gattcttctg gctttcgcag catttgcgtg
    28801 cttttatatt ggggaagaat ttcaaaacgg tacgattcgc aatacgttat ctttaggacg
    28861 gaatcgaatg acttattatg tgtcgaaact tctcgttacg cttttgatca caacacttgg
    28921 cgtggttttg atcacgggac tagccatgct tgcttacaca cttgctttcg gatttggtga
    28981 agttgaggga atcaaaaact atggaaatta tgttctaaaa gtatttcctg tattgcttct
    29041 gctgacatta gggacattat ctgttccagt ggctttgaca ttcatcacta gaagtaccag
    29101 tgtcagtttg cttctttctt ttctttatat catgggaacg gccttcattc ccggtgtttt
    29161 cgcaaaaatc aaaggcttgg aatttctaac ggagtggttt acggaaacgt ggctcatgta
    29221 tacggacttt gcgcaacaag caacttattc gcaagcaccg aaaatgattc tggtcagtct
    29281 agtaacaatc gtggtatcat cagctctagg aatgtttatt ttccaaaaat ctgacattaa
    29341 gtaggtggtc ctgctggaaa ctattttgat tatcgaagac gatgaagctg ttcattcctt
    29401 actggaggag gttcttgaac agagatacaa acttcttgat gcttattcag gtacggaagg
    29461 aaaattattg ttaagcacat atcctgtcga tttgatcctt ctagatctca tgcttccagg
    29521 gctttcaggt gaagcattgt tagcagaaat ccgacagaca tcgaatgttc cgatcatcgt
    29581 tctttcagca aaaagcgatc aaagagataa agtttccttg ttggcagcag gagcggatga
    29641 ctatgtgacg aaaccatttg atatcgaaga acttttgtta cgaatcggca tccaacttcg
    29701 tcatcagact tctgtccaag taaaagatct gtcgcaacgc atctattaca aggaaatctt
    29761 actggacaaa gagtcgaggg atgtaaatgt gaatggtgtg gctgtccatt tgacaggaag
    29821 agaattcgat ctgctgaagc tttttttaga acatccgaaa aaagtatttt cgcgagcaaa
    29881 tctttatgaa acagtttggc aagaaccata ttttgatagt gagaaaacag tgaatatgca
    29941 tatttccaat cttcgaggga agctagcaaa ggcacatgct gtgtatattc atacggtgtg
    30001 gggagtgggg tttagatttg attaaatggc tgttgcttct cgttgttatc tttatgggaa
    30061 gttttatttt cttcctgcaa agggaaatca agagattaac taagaaaatc gccactcttt
    30121 ctgaacgaca agaatatggt ggtcgtcttt cagtcgaatt tcgagaaaag aatctaatgg
    30181 acctagtaga tgcactgaat acatggttgg atgaaaatga acaaaaagga caagcatttc
    30241 aagaaatgga agaaaacatc agactatcga tcgtcggcct ttctcatgat ctcagaactc
    30301 ctttgactgc tattaacggt tatgtacaat tactggaaca ggtagaggat acggagaaac
    30361 gggagaagta tttaacgatc atcaaacaat ctgttgcaag attactcagt atgacggatc
    30421 agttttatga tcttgctcga atagagacga agcaaaaaga aatggatctc catacgcttt
    30481 ctttttctaa agaggtggaa gaagtatttt tctcttttta tgaacaattt tctgagaaaa
    30541 atatcgaggt agcgttcgct gaacactcga taaatacaac tgtattagca gatccgctca
    30601 tgttgctgcg agtgctgcaa aatatcatac agaatttatt gcgttatgca gatggacaag
    30661 caaaggtgac attttcaaaa gaggatccat tcattcgatt aacagtggcc aatcctatca
    30721 aaccaggaac gcaaacctct gttgaaaaag tcttccagcg tttttatacc gaaaatacca
    30781 gccgcacaaa tacggaatct agcggaatcg ggctttacct gtcaaaaaaa ttggtggaag
    30841 gaatgggagg agagatgacc gctagattag acgggggcat cttctccatc tccgtcaagt
    30901 tgcggagggt ttaattatac aagattagtg agaactagta gaaaaatgt
//
LOCUS       DANJMI010000034        29138 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000034 DANJMI010000000
VERSION     DANJMI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.034"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(<1..1031)
                     /locus_tag="PWU81_001820"
     CDS             complement(<1..1031)
                     /locus_tag="PWU81_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1110517.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSVVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="PWU81_001821"
     CDS             complement(1113..1802)
                     /locus_tag="PWU81_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL1110518.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="PWU81_001822"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="PWU81_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL1110519.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="PWU81_001823"
     CDS             complement(2329..2832)
                     /locus_tag="PWU81_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL1110520.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="PWU81_001824"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="PWU81_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL1110521.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="PWU81_001825"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="PWU81_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL1110522.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="PWU81_001826"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="PWU81_001826"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDL1110523.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="PWU81_001827"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="PWU81_001827"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDL1110524.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="PWU81_001828"
     CDS             complement(6444..7463)
                     /locus_tag="PWU81_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL1110525.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8541)
                     /locus_tag="PWU81_001829"
     CDS             complement(7636..8541)
                     /locus_tag="PWU81_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL1110526.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="PWU81_001830"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="PWU81_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL1110527.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="PWU81_001831"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="PWU81_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL1110528.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="PWU81_001832"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="PWU81_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL1110529.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="PWU81_001833"
     CDS             9754..10398
                     /locus_tag="PWU81_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL1110530.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="PWU81_001834"
     CDS             complement(10454..10957)
                     /locus_tag="PWU81_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL1110531.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="PWU81_001835"
     CDS             11554..12519
                     /locus_tag="PWU81_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL1110532.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="PWU81_001836"
     CDS             complement(12618..13790)
                     /locus_tag="PWU81_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1110533.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="PWU81_001837"
     CDS             complement(13777..14922)
                     /locus_tag="PWU81_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1110534.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="PWU81_001838"
     CDS             complement(14936..15868)
                     /locus_tag="PWU81_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1110535.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="PWU81_001839"
     CDS             15985..17064
                     /locus_tag="PWU81_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL1110536.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="PWU81_001840"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="PWU81_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(17789..18016)
                     /locus_tag="PWU81_001841"
     CDS             complement(17789..18016)
                     /locus_tag="PWU81_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL1110537.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="PWU81_001842"
     CDS             complement(18119..20707)
                     /locus_tag="PWU81_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDL1110538.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="PWU81_001843"
     CDS             complement(20727..21386)
                     /locus_tag="PWU81_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL1110539.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLLNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="PWU81_001844"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="PWU81_001844"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL1110540.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="PWU81_001845"
     CDS             complement(22159..23025)
                     /locus_tag="PWU81_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL1110541.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="PWU81_001846"
     CDS             23134..23775
                     /locus_tag="PWU81_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDL1110542.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="PWU81_001847"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="PWU81_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDL1110543.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="PWU81_001848"
     CDS             complement(25216..26115)
                     /locus_tag="PWU81_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL1110544.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="PWU81_001849"
     CDS             complement(26117..26914)
                     /locus_tag="PWU81_001849"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDL1110545.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="PWU81_001850"
     CDS             complement(26892..27596)
                     /locus_tag="PWU81_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDL1110546.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="PWU81_001851"
     CDS             27727..28302
                     /locus_tag="PWU81_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL1110547.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="PWU81_001852"
     CDS             28420..29097
                     /locus_tag="PWU81_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL1110548.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8576 a         6171 c         5239 g         9152 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactac acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actatttaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa
    29101 aggctctttg tcaaatgatg ttggtaaaga gaaatctt
//
LOCUS       DANJMI010000035        27894 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000035 DANJMI010000000
VERSION     DANJMI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.035"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(<1..176)
                     /locus_tag="PWU81_001853"
     CDS             complement(<1..176)
                     /locus_tag="PWU81_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110549.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARV"
     gene            complement(343..2313)
                     /locus_tag="PWU81_001854"
     CDS             complement(343..2313)
                     /locus_tag="PWU81_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110550.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(2328..3194)
                     /locus_tag="PWU81_001855"
     CDS             complement(2328..3194)
                     /locus_tag="PWU81_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110551.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(3184..3417)
                     /locus_tag="PWU81_001856"
     CDS             complement(3184..3417)
                     /locus_tag="PWU81_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL1110552.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(3429..5591)
                     /locus_tag="PWU81_001857"
     CDS             complement(3429..5591)
                     /locus_tag="PWU81_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110553.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYQKEEKEAKAKK
                     AKVKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEEVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLLIPDDPNMFESEVEEATDFYSAVEQLEVATN
                     GLERLAETFNSSEEVEFEALAEDIPDEKEALFADDDKPTEAENQEVSIEETQTQIQTE
                     TYQQSKIDEKPESQSDKENYVYEVEKIVSESDVPKSLDADFLEKNFEQIIADKFQPVV
                     LDEINDPVDLNEEMLSIAELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGK
                     QMLADELLRIEQATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSSIIRKDYDMA
                     KQAYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQN
                     QEAKEVVVERIINSLKPEIENSLTDVQEKTLEIVEKTKSENEAEFHDTKEKITDVVVK
                     YFANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYRSNSEKLAEQS
                     EALREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTIL
                     GSSYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVIS
                     IDGKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(5591..5833)
                     /locus_tag="PWU81_001858"
     CDS             complement(5591..5833)
                     /locus_tag="PWU81_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110554.1"
                     /translation="MVFDREKMLAHANEVLMSGLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTIDRKKGVLQ"
     gene            complement(5943..6281)
                     /locus_tag="PWU81_001859"
     CDS             complement(5943..6281)
                     /locus_tag="PWU81_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110555.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNEQLG"
     gene            complement(6680..10321)
                     /locus_tag="PWU81_001860"
     CDS             complement(6680..10321)
                     /locus_tag="PWU81_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110556.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFTRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLIDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGQDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDENGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEDDEITLDQTHQELRNEMSGEDVSDRVHRELGNGASGESQSRELNSNFDNSA
                     IRNDTALKDRESLSKETDKEQSDVKKKFIPTPNINQTDKYETNNGLGMSKDNQSIREP
                     ELTNDNNRTNNSKSTSDNSGKSNQSTKKPSTAKSAVEYLSKTGKNVYTSADKALAKSP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            complement(10324..12273)
                     /locus_tag="PWU81_001861"
     CDS             complement(10324..12273)
                     /locus_tag="PWU81_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL1110557.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPKKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(12315..13373)
                     /locus_tag="PWU81_001862"
     CDS             complement(12315..13373)
                     /locus_tag="PWU81_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HDL1110558.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(13492..13947)
                     /locus_tag="PWU81_001863"
     CDS             complement(13492..13947)
                     /locus_tag="PWU81_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110559.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(14531..15334)
                     /locus_tag="PWU81_001864"
     CDS             complement(14531..15334)
                     /locus_tag="PWU81_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL1110560.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            16314..17537
                     /locus_tag="PWU81_001865"
     CDS             16314..17537
                     /locus_tag="PWU81_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDL1110561.1"
                     /translation="MESYQFQIFKLVPSLERRGNRMKITEKNVNPKFEPTESNLKKFD
                     LTVTLSNELVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYAFDQVVNLTSKYAISFFQLFTKDFNLYLANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            17572..18339
                     /locus_tag="PWU81_001866"
     CDS             17572..18339
                     /locus_tag="PWU81_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL1110562.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            18531..18929
                     /locus_tag="PWU81_001867"
     CDS             18531..18929
                     /locus_tag="PWU81_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL1110563.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(19236..19610)
                     /locus_tag="PWU81_001868"
     CDS             complement(19236..19610)
                     /locus_tag="PWU81_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110564.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(19632..20009)
                     /locus_tag="PWU81_001869"
     CDS             complement(19632..20009)
                     /locus_tag="PWU81_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110565.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            complement(19999..20394)
                     /locus_tag="PWU81_001870"
     CDS             complement(19999..20394)
                     /locus_tag="PWU81_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL1110566.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            complement(20461..20646)
                     /locus_tag="PWU81_001871"
     CDS             complement(20461..20646)
                     /locus_tag="PWU81_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL1110567.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(20728..20982)
                     /locus_tag="PWU81_001872"
     CDS             complement(20728..20982)
                     /locus_tag="PWU81_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110568.1"
                     /translation="MNYTDEEQITLEYVFIDEEGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSDDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(21004..21249)
                     /locus_tag="PWU81_001873"
     CDS             complement(21004..21249)
                     /locus_tag="PWU81_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110569.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            complement(21256..21540)
                     /locus_tag="PWU81_001874"
     CDS             complement(21256..21540)
                     /locus_tag="PWU81_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110570.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQVYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(21643..21825)
                     /locus_tag="PWU81_001875"
     CDS             complement(21643..21825)
                     /locus_tag="PWU81_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL1110571.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKATGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            complement(22031..22570)
                     /locus_tag="PWU81_001876"
     CDS             complement(22031..22570)
                     /locus_tag="PWU81_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110572.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKIYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKRQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            complement(22683..22949)
                     /locus_tag="PWU81_001877"
     CDS             complement(22683..22949)
                     /locus_tag="PWU81_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL1110573.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(22951..23172)
                     /locus_tag="PWU81_001878"
     CDS             complement(22951..23172)
                     /locus_tag="PWU81_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDL1110574.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            23398..24216
                     /locus_tag="PWU81_001879"
     CDS             23398..24216
                     /locus_tag="PWU81_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1110575.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            24220..24516
                     /locus_tag="PWU81_001880"
     CDS             24220..24516
                     /locus_tag="PWU81_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110576.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(24698..25051)
                     /locus_tag="PWU81_001881"
     CDS             complement(24698..25051)
                     /locus_tag="PWU81_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110577.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKKKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(25071..26126)
                     /locus_tag="PWU81_001882"
     CDS             complement(25071..26126)
                     /locus_tag="PWU81_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HDL1110578.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYKNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            26342..26740
                     /locus_tag="PWU81_001883"
     CDS             26342..26740
                     /locus_tag="PWU81_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110579.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            26743..26916
                     /locus_tag="PWU81_001884"
     CDS             26743..26916
                     /locus_tag="PWU81_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110580.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
BASE COUNT         8627 a         5017 c         4254 g         9996 t
ORIGIN      
        1 aaaactctcg ctttttcttc atcagtaaag tttttataca actgattaca ataatgaggt
       61 ttttgaagtt tctgaatacc tatactaact tcaaatcctt cttcatgttg aattaggatt
      121 acttctgtgt ctctacctag aaatattcga tctaaaattt ttatgtttgg tttcattatt
      181 taacctccac tataattatt tcactcagta aggtttcact ttatgaaatt tctatagttc
      241 tcttttcaca aaacgtacca tgttctctaa gactgttttt agtctcaaat atacatgttt
      301 tttcttatcc tttccttata cactcaaaat caaagataaa acttaaagaa taaactctcc
      361 aataacaaaa ttattagtca gatccactgg tctcctgatc tgaaatttat tacttcttcc
      421 tgttgtcaaa tactgtctca aatcaacaga aagtttttga tcatcttctc cttttctagc
      481 aggagcaact tttttttcat acttcattaa ctctccactg gtcatctttc caatgatagt
      541 gtaattaaaa tcactttcaa aactttggta aatttttcca tctgtattga atatatcagc
      601 tgtttcaata tcttctttgt attcattgtc ttctcctgaa ccgattgtat caaatagaaa
      661 ggcaagacga attccatctt cttcaagcat tgttaatctt tttttaatta gcccaacggt
      721 ttcaatagat aacttatcga agccgtgaat cataacaata tcatcttttt ttgcttgtga
      781 tgtgatgaaa tcaaaggtat ttaaaaattg agcttccata acattggttt ctgctcttag
      841 atcacctaag ttatagtaaa actgtaattt gtcactttta ccgatttctt tcagtgtcgt
      901 tctttgatta aacatcctag agaagctgtc taatgctgtt ctcaatgtgt cttttaatac
      961 aataatacga tcgacttctt tttcagtacc agattttttg gcttttgttt cagcattagt
     1021 caaatagcta ataaaatccc caagaactgg cattgtatca ctactgttca ccaaaagttt
     1081 tgaacgttct tcataattaa tatcccaaag tttctgactt atataaaagt tacctaattc
     1141 ttttttcaat aaaactctag ttgcagaatc taatgttctg cctgctaaca aataaaaaat
     1201 ctgagttagt ttatcgagat tccgtctata taaatcacca atgtcctttt gattaccaaa
     1261 aagctccaaa gagttaagac cttcttcagc caaattaacc ttttctatta cactgtttaa
     1321 cgaaggtttg gcaacaaatg ttctaccagt tggatcacca aagtaatcaa atttatttaa
     1381 gacaatatga aaagttcgtt tatgatttgc cattgcatta ttagcaatca attgccccca
     1441 aagtgatgat gctgagattt tttcatcata gcctttaatt gtagcgtttt tgtgtgatgc
     1501 cactaaaact ttacttgtaa aagaatcatc cagtgccata aaagctacac cacttgcgta
     1561 gctagtagac atagtgccga tagcccatcc attcgcatca tctaaacctt tgcgaattgc
     1621 atgatcattt cctgcaaaat ccgaactcat agacgtatac tcatagacat tcccgattgg
     1681 ttcacttaaa agtctatcaa acagatcacg ttgatctcct ccatatgaca taagctttaa
     1741 tccatacatt ttttcatcat acaaccgttg gagtttaatt agctgttcgc tgattttatt
     1801 aggagaatcg gatactaata ggatttctag cttccaatca ataattttat ttgccatgct
     1861 gtctagtaaa gatgctgtat tcaaatccat aatccgcata acagttttgc taatttcagc
     1921 atctgaattt tttccttcta aattattttc ataactattc gttgatcccc caacattctc
     1981 gtgcattatt tcttgttgat ctttttgtga cactggctta tctgcttcaa ttagataggc
     2041 ttttacccct tctactctca cttcaggaat agctttaacc atccaaccaa atccataatt
     2101 tctattcatt ccataagtat tcactaattt cacaattgtt ccccaccgct tacctgtcct
     2161 aatgtaagat ggtgtaaatt gataaccaac taaagatgtt aaatcactaa caaaggtatt
     2221 attcactacc tttgaatttc tagttttttg ttctttttta gtttttctct tttttttgaa
     2281 atttaatagt tgtgatctac ccataatttc catttaatct ctcacctcta tttcagtgca
     2341 taaatatcag ataatatttc ttttgtttgt ttagctgaca actgagtgac actataataa
     2401 agtcctctac tcgtatagtt ttctaaacgt tccacacctt catccaatac catcttagat
     2461 tgagatttca agataagata ttggattact gttgttttca caccatcaat atattctgat
     2521 acaaatcgtt cttgctgatc tattatgtca aagactgctt tgttactcct tgcatttcgt
     2581 ttttgtattt tcatatttcg agtttgtctt tccgtattct gaggttgaga tgaagtgatt
     2641 ttgatttcag ctaaggaacg atctctggta ttttgatatt gtattgattt cgattcctga
     2701 gccataactt cttctggaaa agcggttagt gatgtagccc catctagatt gaacattaat
     2761 gctacactcc catctcgata tacgattgtt ccatcttgtt tcacttttaa aatctctgtg
     2821 cctcgaaatg gtttcttttt ccccatagat actcttgctg aattaactac tgatttgaac
     2881 agtctaatag aaccagaaat caatgaacca ctagatggat caataaacat atactggcta
     2941 attagaataa gaaatccaat tatcattaat aacgttggaa caaaaataaa tttcagttta
     3001 atcgtatcag aaactaagta aacaattgat ccaagaacaa cgactgcaac aactatagct
     3061 gatacaatta atctttttgc tgtaacagaa taggtgctat tgacatgctt ttcaagccat
     3121 tcaactctga caattgtttc agaaggtttt tgatgtaagt tatgtttaaa tccatagttt
     3181 gagttatctg ccatcagaaa tacttccttt cacgttcaac ttacggtaaa cacctacagt
     3241 tatcactatt gcaacaataa gcaagccaat taaagatatt gacttgctca ttgtctcgtt
     3301 cgtttgtggt agttcattta cctgatacac gagctgtgct tcctgtaagt gctgagaaga
     3361 ggtcatgttt tcttgattgt tttgaattac tggataaact attttttctt ctcgcataat
     3421 agccctcctt agttattatc agataagtga gcttgaattt tgtactgatt tccgtctgga
     3481 gttttcacaa ctgctttttc tccatcaata gaaatcactt cagccttagt tgttttccca
     3541 tcaatactga tgacaacatc tttcccaact ttgaactctg tagttggtgt tgcttcatag
     3601 ctactatttg ttgaagcaga gttcgtattt gtcccaatcg aattttccac tctcgaaata
     3661 gatgtttgag tttgagcctg tgcttgttga ttattatgac taataaagta ggacgatccc
     3721 aagatcgttc cgcaagccat tgcagcgaca gaaatgatag tagccatctt tttacttgta
     3781 aaatcacgtt ttgaacttga ttgattttct gtagcaactg ctaatgattt cgttttttct
     3841 gccatttgag ctagatcact tcgggaacga gataagtctt ctcttaaagc ttcactttgt
     3901 tctgccaact tttcgctatt tgatctataa ccctctgcta aatctttgac ctctttcatt
     3961 cgatctttat attcttcgat agttttttgt tgctctacaa gttttgtttc taattctttc
     4021 gtgttgttag ctacttgctt ttcaacttct tctttgttcg caaaatattt gacaaccaca
     4081 tctgttattt tctcttttgt atcatggaac tcagcttcat tttctgactt tgttttttca
     4141 acaatctcta acgttttttc ttgcacatct gttaatgagt tttcaatttc tggtttcaat
     4201 gaattgataa tccgttccac cacaacttct tttgcttctt ggttttgttt caatgcatcg
     4261 tttttaatag attcaattgc ttcttggtac tgttgatcaa gactttcttc agttgctttt
     4321 ctatattcat cttcttgttg tttaacttct ggtaaatgtt cagcatcata ccgttgctct
     4381 agtttcttta gttcagcatc caaataagcc tgtttcgcca tatcataatc tttacgaatg
     4441 atactggatt tttccatcac taaatcatca atcttagatg cttctgcttc gtagctctct
     4501 ttagcaaccg ataatgtatt tgtaaactga ttttgaccat ttaatgaggt tgcttgctct
     4561 atccgcaaaa gctcatcggc taacatttgc tttcctttga ggatcaattg ctccttcgtc
     4621 ttatttaatt cactgttgat tttattatta gcgatagaaa tacgagcatt catactctct
     4681 ttagctaaaa acagcaatgg tttcaactca gctatggata gcatctcctc gtttaaatca
     4741 acaggatcat taatctcgtc taaaactaca ggttgaaatt tatcagcgat gatctgttca
     4801 aaattttttt ctaaaaaatc agcatctaag cttttcggta catcactttc ggatacgatt
     4861 ttttctactt cataaacata attttcttta tcactctggg attcgggttt ttcatcaatt
     4921 tttgattgtt ggtaggtttc tgtttgtatt tgagtttgtg tttcttctat gcttacttct
     4981 tgattttctg cttcggttgg tttgtcatca tctgcaaaaa gagcttcttt ttcatctggt
     5041 atgtcttctg ctaaggcttc aaattcaact tcttcagaag agttgaaggt ctcagcaagt
     5101 cgttctaatc catttgtagc tacctctaat tgctccactg cgctatagaa gtctgtagct
     5161 tcttcaactt ctgattcaaa catattagga tcatccggaa taagtaataa ttgaccacta
     5221 ctatccataa aacggacacc tgctaatagc ttctctttaa atttttctgg tagatcagct
     5281 acataattca aacttgactt caatagaacg acatactttt cggaatcgag aatagcatca
     5341 tcctcatcta taacttcctc actgttgagc ttttgtacat cttcaattac catataattt
     5401 ttctttggta aatctttggt cagcaacgca tcgaattctt tccatacctt taccttagct
     5461 ttttttgcct tcgcttcttt ttcttctttt tggtacactt cgagtaattg tggcttcaaa
     5521 gagagtgcaa ctacaggaac tgtttctgcc acataattaa tagattcggt aatatctttg
     5581 aattttgcca ttattgtaaa actccttttt ttcggtcaat agttcttttc tgtttgtcta
     5641 gcatatatac atatgctttt tcgaatttaa taagcgtttc aagtctggct ttttcaatct
     5701 ttttactccc attccgataa tcatagaact ggttaacatt catattcatt atctttgcca
     5761 gttcggtacc tttcagaccg ctcattaata cttcgttggc gtgagctaac attttttctc
     5821 tatcaaaaac catcttacat ttactttctt ccctcacttc taaactacgc ttggagcgta
     5881 gttttgtggt aaaaagtaaa ttgaacaaat cggcatatta tccaaatttg ttcaattcac
     5941 ttttaaccta actgttcatt ttttggaaat tttgaaacta gatagatacc aacaactgga
     6001 ataagtgctg atataatatt agctttttgg ttcaaatcat tacctgtttt agtacccatt
     6061 gtgtaaagca taacggttgt aattacaggg ataataaaga ttgcaataat cgccccaaca
     6121 tattttaatc catctcccca ttttgcatca ccaaaaaatt tcattgcagt gaatcctggt
     6181 ttcaacgaaa aaacgaatgc caagaaatac aaaacaactt gtactaaagt tttcatatca
     6241 ggcgtcattc cgctaaccaa atttttaata gaatcgaaca tataaaaacc tcctttttaa
     6301 tatttttaat aagttaatta tatcatcgac aaatcttatt tgttcacttg tcaatgatct
     6361 aactaagaaa aagacgtctc gatatttaca tctttatgtt atcataataa cataaatttt
     6421 tgatgattaa aagagatttt ccattaaatt atttcatttt ttttattggc taaaaatgct
     6481 ggtttatcaa gtttttgttt agataataat tctgttcttt accagcataa gcccttttta
     6541 attaaacaac tttgaacagt aaatatttaa ttcatttata gataatttta aaaatttttt
     6601 tgcataaata gaaatttttc ttgacatctt cagttaaaaa taaaagtaaa aagaaggagc
     6661 aaatcaatgc tccttctttt tattcgtctt cagacttatt ttcagttgat ttctgattac
     6721 cgtattttac aatatagtcc ttgactgaca tggtagattt cttcactact ttaccagtct
     6781 tggatgctcc tgtagcagta gccttggcaa ccttagccgt tcctttacct attttaatag
     6841 cttcgttttt agcttcatag gttatccctg ctagactacc ttttggagat tttgctaaag
     6901 ctttatctgc tgaggtgtaa acatttttac cagttttcga tagatattca acggctgatt
     6961 tggctgttga tggtttcttg gttgattgat tagatttccc tgaattatct gaagtagatt
     7021 tagaattatt tgttctatta ttatcatttg tcagttcagg ttctctaatt gattgattgt
     7081 ctttcgacat tcctaatcca ttatttgttt catatttatc tgtttgatta atattaggag
     7141 taggaatgaa ctttttcttc acatctgatt gttctttatc cgtttctttc gatagcgatt
     7201 ctctgtcttt aagtgctgtg tcattccgaa tagcactatt atcaaagtta ctgttaagct
     7261 ctcttgactg actttcacca gatgctccat ttcctaattc ccgatgcact ctgtctgaca
     7321 catcctcacc actcatttca tttcttagtt cttgatgagt ttgatctaat gtaatctcgt
     7381 catcttcatt ctcaggaatt gcaacagcaa ttggcgtatg ccgttgttgt tcgagttctc
     7441 tatttcgatc tgccacaagc gcatctctcg tctcaatgat attcccattc tcatcgacta
     7501 aatcgccatt cacattatat ccatcgctgt gtttaccaag tttattacca aactcatcaa
     7561 caagatttcc atcttcatca actaaacgcc ctttttcgtc aactaaacgt ccgttactat
     7621 cctgaccaaa atcaccattt agcaacctgc gtccattttc gtcaacaaga tttccatctt
     7681 catcaactaa gcgacctttt tcatcaatta aacgtccttc gctatccata ccaatattac
     7741 catcttcact atcttccaat ggtttaccat tagtatctat aaggtttcca tcttcatcga
     7801 tcaaacgccc tttttcgtca ataaagttgc ctttagcatc tttaccgtat ttactatctt
     7861 ttaatatttc accattttca tcgacaaagt ttccgttttc atcaaccata cgtcctaaat
     7921 gatcaatata ttgttgggag ttattacgta gttgatcttg acgaactaaa tctttgaaag
     7981 aggtattatt gattgttgaa ccaactccgc caccgttgaa tgacattcct tctggtgttt
     8041 caggaattac tttatcagca atggacttag catcagcatt gctatctccc atattgttca
     8101 aacgttcagc cattttttgc gctccaactg ctccaagacc tcctgcaacc atatctttta
     8161 ctgagctgtt gccaccatca taatagggat ttttagctcc ttggcttttc ttgattgctt
     8221 tagaaacgcc gtccacaact cgcacagcat catttctatt accaaataat aacttagcac
     8281 gttcatcaaa cccaaacgct acttcaagtg gtaagccaat aagcatatta atcaaattat
     8341 ttactttttc gcctttttta aaaactaaaa gatatcctac acctaaaatc atagcagatc
     8401 gaacaatatt tgctacgcca tcgccaacta atgtattagg aatgaatccc acagcagtat
     8461 caccaaatgc acgaataaaa gtaatcagaa agataggtaa ttgtagtccg atcagagatg
     8521 ttgtaacaac acctaatgaa attgccatca cacctgctcc tgatgcaagt gatcctgtta
     8581 gaactacttc ccaaatccga gtaaattcac gtttaaatgc tttcacgata ttcacattga
     8641 acatcgccca agcacatgct aagtaaagaa ctgctaaaac gattgattca gcgttcatcg
     8701 ctagccacga gccaaactta cccatcgctc catgaccaac taaggatgct gtacgccatt
     8761 ctgtttttac tgttgacata ttcttagcac tgccttgaat tcctgaagga gcgatattat
     8821 acgcataaaa cttagctcct gatgtatcaa gtgccgattg gagcaataaa gctaccgact
     8881 gtgtgctaaa agttgcattg ttagcaacgc cccaaggttg agtagcatca aagttatgcg
     8941 ctgttccttt tccatcaggc ttgcgattgt cgtctgtcac attttgattt gctgaccaaa
     9001 tatatccatt taaattggtt ggtttcttaa ttgcacttcc ttcttttcca tcccctaaat
     9061 tatagcctgt ccaaacagca ggcgctcttc ccatataagc atcattttcg gcgacattaa
     9121 catcaccggc ataagtatta atattgaaag tatcattcga tgaccattct tttaatagag
     9181 ccataccgac tgaattagaa tctagatcat tattttgtct ggcataactg ctagtattga
     9241 tcgctcttat cattgcagat tttttagatg gttcataatc tgtatcaacg aacttgattt
     9301 cggatgtagg ctttccgtca ggtacatcac caccagtatc agatgatgga gcaagatttt
     9361 gagtagaagc ccaaagtctt gtgttaatga tgtagtcaga aacaacattt gttggtatat
     9421 ctccatcttt ttttgctgac tggatgccgt tgatataaac tcccaatagc gatcctgcca
     9481 ctggtaagaa tataaacaat acaaaaatac gaacaatcca tttttcaagt actggtgata
     9541 atcccttcga gttaacacca tctttcgaca ataatttcat tccttgataa cctagtattg
     9601 ctgtgaaaaa gagcatagat aagcttgcta tcgctttaaa taattcatta tttaatccca
     9661 aattgttaag caagctagtt aatgctttag ataccggatt agatacatcc gatccacctt
     9721 ctccccaccc cataagagaa aatatattca tactcatgaa aaattttcca aggagttcat
     9781 ttatggattc agcaatatta gaaaggatta aactaattga tccgataaaa ctaccaatcg
     9841 tcctaccaat acttcctaga atggatgatg cttgacttgc aatagcattt cctgagactg
     9901 tttcaatata tccgtagtaa ctataagttg ctaaagtaga tgctagttgc gtacctgcat
     9961 taccccctgc aatcgaatta ccttgtttag aaatgtcaga ataagggaaa tcggcactga
    10021 taccaccttg tcctaacaaa ttcatttgtc cagttttagc ggcactatca ttgacttctt
    10081 tatctcccat taggatacga gcatatagtg tccacattcc ttgttctgcc ggtttacttt
    10141 ccgcataagc aacgttctta tttataactg tttcttgttt gctatcttga ttgtcaggag
    10201 ttggatcatt cattccaaaa ggagcgccta aagtaactgc taatactcct ccaataacta
    10261 gacctcttac gacccatcgt tttattctaa ggttttgtag taaatatttc ttgaatttca
    10321 tagctacgcc cctccaatcg aaaatacaat cttgttttct ttactttcag caacggaagg
    10381 ctttgcaata atttcagcat tagaatcacc ttttccttca aacgctccgg cttcactcaa
    10441 cttaacatcg aatatttttg cgtttggata agtaattatt gcttcagtaa tcgcttcttc
    10501 aaactcagat tcaactaaat gcaagatatt ccttgcaccc cctgatttag aatcctcttg
    10561 tgaagccatg tcaaaaacta agtgcatgga gagatcgtct gcataaaaat tttgaccata
    10621 tactctttct ttcttaggtg gaagacgtaa atctatattt aaatgttttc gtactaattc
    10681 agcactcttc aacatttcat tataagcaat ttccagaaga gtttgttcgg ataaagccca
    10741 aaatggaact attcgtccaa atcggttaat aacttcaggc tttagacctt caccaatcaa
    10801 ggcttgtttg atccctgatg cgacttttcg gtattctctt tttagttcag tgctgtattc
    10861 attagaattt tccttgtttg taacacgaga tagtgagcta tatcggttta acgaatcgaa
    10921 catctccgca cctgcatttg ttgtcgcaaa taaaattgta tttgacaatg aaacggtgtc
    10981 gtgaacttct cgatcatccc atcctctttc agtaaaactt atcctacctt catctaaaat
    11041 tgataaaaag gcatcgagtg tttctacacc agctttctca tattcatcac acacaataat
    11101 cgcagaagga tcatgcctta catgagtacc tataagtcgt ctaaaacgca ttgcatcatc
    11161 tttggttttg tagtccggca tattaactaa gagaacttta ttttcatcgc cataaagtcc
    11221 tcttgccacg gctttagcca ttgcagtttt accgacccct gttgatcctg taaacagagc
    11281 agttgctttt ggttgctttc gacctttaaa acgatcaaat ccaagcttca tacgtttaaa
    11341 caacattgtt atagtataaa ctgctaacgg ttgtcctttt atggacttta attcctcttg
    11401 aagtttttcc atatcaaaat cagaatctag ctgaatatta tacttaccaa atatttgcaa
    11461 tagactttct cgattataag catttccgtc tactcgatct tttgcaatca aatcctccat
    11521 tacagcaatt gatttagccg gttcggacaa atcctcacga taaagtttgt ttgcttgtac
    11581 gatttcttga agtaattcat caggaatcct ttgcgataat ctaaaagaag cctgaggatc
    11641 atctttaaca gaggatgtat ttagccattg tctcaaaata tcgacgacaa catcgctttt
    11701 tagttcattc aattctaacg gttgtagccg tctccctaag gcatcatctc ttttgatata
    11761 gttttcatat tcttttcttg tagtagcggt aatcatttga agtgattcat ttgctaacgg
    11821 ttcttttagc gaatcacccc caattttatt gccactccca aaagagctaa caactttatg
    11881 aaactcgtca atgaagagga caaattcatt atctttgtcc tcagctttta aaattcgctc
    11941 atattctttc aatcttggaa ttaatgtttc taaacgtgtt tggaggacat catctccccc
    12001 aacacgcaat tgtccgattg ataatttata tacttgtact ttctttccat cttgtcgctg
    12061 tctttcagcc cattcacttg caagtgctgt tttacctgtt ccggcttcac ctaacaagat
    12121 agcttgcggt gtttccggtc tattcaagat cagtgacaac tcatggagtt cgtcatcacg
    12181 tcccactaac ttatgttttg gtggtttcat tggtgtgaga gcatgattaa attcggtttg
    12241 aatattctta aaaaactcgg tactattttc catctgtttg tgttcacttc ctctaaaata
    12301 agaataattt acttctagtc agtcaaagtt aggtacttaa atgtcgtctt catgtctcca
    12361 aaagaatctt tgttaaagtc agcatttttg tattgatcaa tcaatttttg tgtatcattg
    12421 attgctttaa tttcggtctc cattcgactg tctagttgac gaatactttc ttgcgaatct
    12481 ttatcgtcag gattttgttg taaacgttcc agatactcag cttttgtggc ttcaattttc
    12541 ttgatgttag tatttgaagc atttaactga tcttctagct tatttacaac attattctta
    12601 cttgtaactg ctacaacttt tgaataatca atatcgccat ctttggtaag aaagcttcct
    12661 tgatagacat tagttgaatc tgaatatgga tttattctga aattgatatt atcaggagta
    12721 ttaacaggtt ttgctttttt cccaaaagaa attactcctt cattattaac atcattcata
    12781 gaatattgag aaagtgcttt tgtaactgaa tctgcttcat tcacattgac tttggtatca
    12841 tcgtcagctg tatcatcacc agaaattagt gatacttggt ttgcaataaa aacttgataa
    12901 gctctttcct ctaatttacc ttttaggaat aagaaagcat ccccatcagt acttaacatc
    12961 ccatagctca tttcaagctt cttaggacga tctttttctt ttgttgccat atataagtta
    13021 tagttttttc cttgcgtaga taacaacttt ctagcgtttt gggagtaacc aagttttact
    13081 actgttacat ctctattttg atctgtccac acatctgaaa tagtgatttc tgctcctgat
    13141 cgagcaaact ccaatgaaga ccctataggt gttgtattgt tcacgagata ataattatag
    13201 tttcctaatt gataaatgta gctacccaag cctgttaata aaaaaagcac tcctagaaaa
    13261 gttgcaagca taatgcgtaa tccgtgacgt gttctcttat ccttatcacc aagcaaccct
    13321 ccaataatac tgtattgatc tttggtagat gttgaattga cgtttttttc catcagtttg
    13381 tctgccccct ttcataaaag agcaaacaat cagacgtttt ttcggtaatt ctaggttcgg
    13441 gattttgaat catttcggta tattcttccc cattaatatt taacacaaaa attatcttcc
    13501 tttcttcgtt cctttctttt gctctttatt ttccaaataa tcaatgattt ttgctattgt
    13561 accaagatag taattcccct cagctttgtt ttttcggatt ttgctaacca ttggagtagg
    13621 tacaatctct tcatctctca attcactgaa agatttttta cctagatatt ttccaataac
    13681 agattttaaa aaacgttcaa aatctttctc gtaagttgtt ttcttgcctt ttagttcatg
    13741 gttcatattg tcaacgactt ttgcggtttc aataattgtt ttagttctca gcttaataat
    13801 ttcatctgga attttcaatc ctcgtaattt catcgttgta ctaactggta acccatgctt
    13861 tactgcatca gcaatactaa aactttttag atcatctagt aagctctcca tatagttaac
    13921 aaattcatgc caattaatag cattcattgt ctcacccctt cattttttta aaatgaggtt
    13981 cgggcgtaac acatttatgt tattataata tcataataac ataaataaat caatcagcaa
    14041 atttagatat cccttaaagt atcagcaatt attcaacaag atatttattt tagctatttc
    14101 agtcatcata aaagactaat tagcaataaa ataactaata ttatccattt atcagacgaa
    14161 aagaaaaaac ttatctaatt actctatatt aacttttaac aaatagttga cttgtccaaa
    14221 aatgttacct tcaatctcac aaagatatta agatgtttcc taccaaaact gatatcaccg
    14281 gactttttat tctgtgttgt ccaaaagtgt tacctttagt cgatcaacaa attaaaaaaa
    14341 attgtttttt tcttgacttg tccaaaagtg ttacccatag ttgttaaaat ttgtatagat
    14401 tagccattct ttcgaatatt atttagactg aaacttgctt acaaaaaaac ttcttgcatt
    14461 tattgtccaa aagtgttacc catacttttt taaagaagca ggttgtatta aaattacaac
    14521 ctgcttcttc tcaatcttct tctaaccaat tatgcattga aatggttggc aagtttgatt
    14581 ctggctcact aaaagtgaat ctgagccgat caactcttct tcctttcttt gcatagattt
    14641 tttcataatc aaaagtttca aatacctgta ttccgttttc atcaggagct aaaaattctt
    14701 ctttcgcctg ctctagtatt cttttgtcaa tatttgtcat tctataactt gaaggtatac
    14761 ttaatgctct tctaaaatct tcaactttga ctacccaata accatttcgc cactctttat
    14821 ttctgtattg catgagatga cgatacaatt cttttgtgta gctacttctt agttttgtaa
    14881 aattatcaag ttcaaaacga gtaaagtttt tgccaattga gtttaagata aattcaaatt
    14941 cctcatttgc cttaattgta actgtttcct tctgtttatc tatccggaat ttttgaaaaa
    15001 gccacagctg ttcatagacg tctgaatctt caaaacggta ggttaaagta cctagtttgt
    15061 cactcaaagc ttttaacaca gaataaaaac tgtccttttc tcttctgtcg tatttgacca
    15121 aataacgaaa gaggttgaaa tcaaaagtta cttctgctgt tccttgacgt ttcatttttg
    15181 ataaaatagc ccaaaacaca tccatttcaa caggcttaaa tcctcgcaaa gctgttgtat
    15241 tcagaccatt gttataacgc acaattgcat ttgtttgttc tgatactaaa tctacatcac
    15301 tatcaataat cttttcggta acttcttgag acattgatta ctctccaatc ttctttttgc
    15361 gctcctttaa tcatgttaat ctggtactaa ttaaaggaaa caattttttc tgcttgttac
    15421 ctaaattaaa tcacaactaa aggtaacagt caacaaagat ttgttactaa tctaattttc
    15481 tattttttcg ccttccaaat caagaaaacc gaaggtaacg tcttatccta gtgtgatacc
    15541 tcaaatgtcc acttttgtta cctttgctgt ccaaaagtgt tacccttact gtccactttt
    15601 gttaccttta ctgtccaaaa gtgttacctt tatacccctt taatacttac tctcccaaga
    15661 gattaagcct cccctaacag atatattaac agatatttaa cagatatatt aacagatatt
    15721 taataaagga ggattactcc tctcttattt caaaatctta aaaataaaaa atcttaaaaa
    15781 aaactaacac tatctaggag tttattttgc ttgtgtaact ttgaataatt gaaaaaaagg
    15841 aaaaggagga agcttcagct tcccctttta tttatattta taatatatat tttaatcttt
    15901 aaaactttag caaccttcaa aaaggcttta tatcaatctt aaatagattt tagggtggac
    15961 ataaagcgat gcgaacatgg acaaaaagcg atgtgaatgt ggacaaaaag cgatgcaaac
    16021 gtggacaaaa agcgatgtgg tattccgaat atatccaaaa aaagaaaaag tatgtgaatt
    16081 tatttctaaa aactagaaaa atatttgttt tttaattttg gtaattttta taatttcgta
    16141 tttttttgaa aaggattttt atattgaaaa tgttctaaaa tagcgatttc tgctgtctta
    16201 tcaatgaaaa ttgattaaaa aaataaaatg tgttttacag agattgagtt atgttataaa
    16261 tttacgatac atttcttatt gtatcgtatc gaaaacaggt ctatacttat tcaatggaat
    16321 cgtaccagtt ccaaattttt aagctcgtac caagcttaga aagaagaggc aatcgtatga
    16381 aaataacaga aaagaatgta aacccgaaat ttgaacctac cgaaagcaat ctaaaaaagt
    16441 ttgatttaac tgttacttta tcgaatgaac ttgtagacag cattgttgaa atggatgcta
    16501 tgccacaaaa aatttatctt tatgctatag gacatcttga ccctaaaaat ccgcctttta
    16561 ataatactgt taaagtatct aaaaaggctt tatttgcgtt gatgtcttct agttcatctt
    16621 cttcaaaaca ttcaagatta aaacaagtta ttgaaaaatt gcatcagcaa tctgtcatta
    16681 aaattcaaag aactaaacct aacggtaaga ctgaatacaa agtcatctca ccgctagaaa
    16741 ttactgaatt caatgatttt tctgatgcag tatctattaa atttagtagt tcaattatgc
    16801 ctttattaac gaacttagaa gatcatttta cgcaatatgc atttgatcaa gtagttaact
    16861 tgacaagcaa gtatgcgatc agtttttttc aattattcac taaagatttt aatctgtatt
    16921 tagcaaatag agataactta acatggagta caaaatcact tgatagatta aaaaatcctg
    16981 ttttctccat tgcagattta cgaaagaaat ttagtattga gaaaaaatat aaagcttttc
    17041 gagactttga aagacgtgta attaaagaac cgattgccca actgaataaa tacactgatt
    17101 ttaaaattga ctatgagctt atcaagaagg ggcatactta tacaaatata aagttcttgc
    17161 ttacacgtac catgtcagtt ccggtacctt ataagcaaga acaaagagac cctgcttatt
    17221 taaaaggcgc agaagataaa acagagaaaa tgaatcaatc gtacttgaaa gctatgaata
    17281 gcaaatacac taaattacta atcaaaaact ttttgatcag ttctgccgac ttatgcgatc
    17341 ttaaacttat gagccgactt cagaataaag tttatccgct ttatgatcgt ttatccaaag
    17401 atagaggatt tgaatcagtt gtcaatcacc tatcttacgt tgctactcat aaggaggatt
    17461 acacaataaa aaatactgtt aaatatctcg aaaaatcaat tacgagttat ttgccttatg
    17521 tgaaccttta tgattaacaa ctgaaaaaat gcgtgaagga aggaaaaact tttgaaaaaa
    17581 gatgaactga caaagtacaa tgaggagttc ttagcaacaa ttgacatgaa aatggattta
    17641 aagtcgtatg aactttatca attgctgttc agttatatct cacccacaaa gctaaagaaa
    17701 acaggcgtag tagatttacc tgttaaagcg attattaatt acatggatgt tgatcaaaat
    17761 cttgaaacgg aactaaaaac catagttaac actttgcaaa gacaagcagt ttatgaaatt
    17821 tcgaatctag acaaaaaata taagacgtat actactgtat ctgctattga tcggattcat
    17881 tgggatttag aagagcatag tgtatctttc agtttttcag aagatgtaat tcccttaatt
    17941 gtctcaatca aaaagaagta tccgttcttt gtttttgaag acttagcatc ttttcaaaaa
    18001 aaatatagca tcgtgttata caaatatttg gcgatgtatt ataaaaaata tcttatcgcc
    18061 aaaaataaag gagattttga gaaagctgaa atatataaga atcccctgat tgaaatagtt
    18121 aaaattcgtg agattacgaa aacagaaaat gagtataaaa caatatcgaa ttttgataga
    18181 cgtgttttaa gggaatcagt tgaggaaatt aatgaaaaat caatgctgaa tattgatttt
    18241 gctaaagtta gaaaaggcag aaattacaat tttgctgtcc agtttaatat ttctagtgag
    18301 tttgatgaaa aaacttatag agaaaaaaaa ttaggttaat gcaaaatata tcttactcgt
    18361 ttgaagatgc ttcagacgta tgctaagata tatagtgcct tgggagcaag gtattaatct
    18421 cctttagagg ttaattagct tgtgatcgcc tatcaatttt tttggtaggc gattcttttt
    18481 tttgaaacaa aaaagcaaaa attatttttt aattagagag gtgcaatgcg atgaaatact
    18541 ttttttgtgc agtattcgaa tggaaaggaa atgaatatgc agttaaattc ccagattttc
    18601 cagacttata tacacatggt gaaactttag aagatgcttt tgagatggcg caagaagtat
    18661 tggaaggata cttggtgctt ttagagttgc aagaaaaaga aattccatcg cctacgcctg
    18721 ttcaagagtt aaatgcaaga atcaagcgat ctgagaatgt taatttattt gtcgtaaaag
    18781 ctaatacgaa agttgctaaa gctcgaaaca agcaaaatta tgatcgaaaa tcagtgactg
    18841 tggaacacta tttaaatgtt ttagcggctg aagagaatat taatctgtcg aaagctttga
    18901 atgatttgtt gagagaaaag ttgatttgat gtctctcaga tgatcatatt aagcaaaaac
    18961 agcttgtttt aggacgtatg gatttaagaa agacaaatta tcaaagaata tattaaaagc
    19021 tctcagaagc cttctgagag cttttaaatt gctatgtatt gtactgcatc tgctcaaatt
    19081 gattttcttt tcatgcttac catgtatcta attttccatc aagtaatttt tagatatgta
    19141 ttgagtaata aatagcaaat ttgcttgata taagctcgaa ttaggtgctt tgtaatgtgt
    19201 taggataaaa ttatacaaga aaatgtcaaa agctctcaga acgcttctga gagcttttta
    19261 tcggtaggct tgtttaacgt gatcttgaag gatacccttt tgatgtttct ctcgatccct
    19321 gcatcgctaa ttactaagtg ttcttcaagt gtgatgtagg tatataaatt aagcatcaaa
    19381 atggtgtaga taagcaaaaa actccctaaa actaagtgtt gattataaaa atctgactta
    19441 taaaaaagaa ctgagaatgt tagtgttgat tcccaaaaaa gaaacaaaat tttgtatttc
    19501 caaagatagt ggatcaagcg ctgtgcttta cccatctcaa ataatttata ataacctgct
    19561 aattgatcag caagcgtatc gttaacggtt tctgtttgtg tttgtttcat ctttctcttc
    19621 tcctgtttca attaatctaa aatttcagat tgagcaagca actcattgtg gtagtcggtt
    19681 tgaaattcaa ataacaattt tgccttctta acgaggttta aagcgttcgt tagtgattca
    19741 cttaatgctt caacatcttt aactgatttt gcatgtttca ttttttcagt gtgcgtatag
    19801 cagaattcag gtattgagtt aggggttgcc tgttcaataa acatctcaat tcgcaagtta
    19861 ttatcctcaa aaataattag atcattggca aagtgtttgg tactaacgct aatgccagtt
    19921 gtatctttta atagattatt gattaacgtt cgagagaaat agtttaagtg tgtagctttc
    19981 ataggttcgg tctccttttt acttttcaag caactcttct tccagtgctt tttgaagtag
    20041 ttgagagaag tttatgtttt tctccttccc taaaagaact aaccattccg gaacactaac
    20101 attttttcta attgtttttg tatttaagct tcttcggaaa agtttaatgt ttagttggat
    20161 gattactacc acttggttgg ctttcaaagt taattcttcc actgctgatg gtttaggaaa
    20221 ttctgcatct ggtacactat atagcgctaa tcctaaagca tccttagcgt ttaaatatgc
    20281 ttcttcaaga ctatttccaa aggtagatat ttccggtaag tcagggaaaa cgatgttata
    20341 agcttctcct tctttctcga agattgccgg ataaaaaata ttttttgtct tcatttcctc
    20401 gctccttttc tagtgtaaga gaggatagta tgcctaatac caaataggaa ggcaaggctg
    20461 ttaaagccct gcttgcttcc agatattagc ttctgttcct agtggtacat caccattgtg
    20521 gatcggaaca acgaccgttc ttttagtctt ggaattataa aattttgcat gactgccagt
    20581 ttgtccagtc tttttgaagc cgttagattt gagaattttt acaatttctc ttgctgtctt
    20641 aggcatagct accctccctt tacaaaatct attatacaca ttgttgtgta tggaatcaat
    20701 agaaaaagag gatgttttat tgcaaaacta ataaaatgat ttattggttt tttcagcaaa
    20761 ttgaatgtta ttagcgttgc aaatattcat gatttcccaa gtatcatcag aagtataggt
    20821 atctaagaat tcattgacag ttacggttga ttcgtatggt tcataagtca aagcgttctc
    20881 caagtctttt gatgaaataa tcaagacttt ttcaatttcc tccccttctt catctatgaa
    20941 aacatattct aaagtgattt gttcttcatc ggtataattc atattgtgtt ctcctttctt
    21001 gtactaagaa aaatggatat ttccaattgc atcccactcg gcaccattgt ggtataggac
    21061 gtgaccgagc gttacgcctt tgaaatgttc ttctaaatac atccaaacgc attcaaggct
    21121 agtacctgct ttgagattgt agaagtctgc atccaatacc ttttcagaag ttatcggatc
    21181 aatgcgagtt ggtacttcaa taaattcagt ccaaatttct gcaaaagtct taccttccaa
    21241 agtccacact ccctatcaat taattccaaa agcaaataga gaaatcaagc gaccgtcaat
    21301 agtcacttca acaactgaaa atgccgttga aacagtttta acatctacac tttccaagtt
    21361 cccgatgaag gaatctttgt gctttagaat agctttcttt agctgtctgt tatacacttg
    21421 ccgactgtta aactttttga attctcgatc cttgatcagc tgtttaacga tccctgcatt
    21481 aattgtataa ctttctgcaa tagcaacgtg agtaggttgc tgtaaagtag ctgttagcat
    21541 cttgtcactc cttcctaaaa gttaattagc ttgtttacaa atacattata cacatcggtg
    21601 tgtataatgt aaagagaaaa aggtgctaat ggatcagctg ttctaaacaa aagaaaacga
    21661 taaagtaaca aataaagtca gcaaagttaa aaaggatgaa acggctagtc cggttgcttt
    21721 gcttgtgtga ttttcggctt tcttatagat caaataggaa attatcagtg atattccagt
    21781 aaagaataat gtactagctg tcactaaaag aaataggtta atcatacgat acactccttc
    21841 tgtaatattg gtatcagata cgtttaaatg cgtttctgag ctgttttgga tcagatggtg
    21901 atgtttttag ccctacccta aaaacaaagc ccagaaaaga atttaaaagc gttcgtacct
    21961 aatacgttta tattcatata catatacata aacataaacg tgtatacgtt tatgtttatg
    22021 tatttaagtt ttaggctact tccttaacat ctcctgctga tagtcgagtg aaccgagtga
    22081 gtgatccatc ttgtagtcga aaaataaagc tacgtttgag tttatactga tagacagcga
    22141 catacgatcc atcccagatt gagtaagttt tacctgcaac taataagtca tcaagttttg
    22201 gtttattgct ttcttcttta attctatcta gttgtcgctt aaccattttt tcttgatcac
    22261 cgacaaagaa tttcaaccat tttttcggaa ttttcttaaa gtaagcagga ctgtcattta
    22321 agaagtagat tggtttgtac atttcatctc gaaagatgaa gctgtcaata tcgtaggttc
    22381 cttctgcatc tttgaaaagg ataattccat agatcttcgt tactttggta tctacgattt
    22441 ccaaaccatt tttggcgtac cctttctttt cttcgatcac ttcatttgct gatgaataat
    22501 caccatacca tcccataaaa aatcgctcct tttgaaagtt aattagcttg tttacactta
    22561 tattatacac accggtgtgt ataatatcaa gaggatattg ttctttttta ggtaaagtgg
    22621 gatcgtaaac agtgattttg ttgggttaac aaaaaaacag acaagcctta tcagcttgtc
    22681 tgttacttat aaacttcact tctgtgacca atagacaata taagaattgt cacttcatgc
    22741 tcgctaatat cagctattat acgatatgca cctaccctat atcgccagta gcctgattta
    22801 ttagctgtta gacctttacc atgctttctt ggatcatcag tgcctacaag atttttctcg
    22861 atccagttta ggataacctt tgcctgaaac ttatccattt tttttagcgc tttttgagct
    22921 tcacgttcat atcttacttg gtaagtcatc ttataagcct aactctttta gcatatcttc
    22981 gtgggaaata ctttgatcgt tttcctcatg ctctttcatt agttgcttgt aggtggcaaa
    23041 gtctactaac gtttctgctg tttgcaggga attactacgc ataaattcag aaatgttatc
    23101 tccgttgaat tcagcaactt tttcaagaaa atctttttct tctttagata cacgaatact
    23161 tattactggc atagtttatc aactcctttt caattacatt gtactacgat tgtatgacaa
    23221 atgcaatagt tatatatttg aatatataca taaacataaa catatacata tatatgttta
    23281 tgttgtagaa tataaaagca aatataaata taaacacgtt tatgtatata aacatataca
    23341 taaacgtgtt tatagtgtat aatggagtta caaaaggtta ataagggagg tttaatcatg
    23401 gaaaagaagt tgtcaatcat tactggaaac attgaaaaag gtggaacagg taaaacaaca
    23461 acaatctaca atctaggtgg atatgctgtc gaaaatttag gtatgaaggt gctgtatatt
    23521 gatgaagata agtcgcaaaa cctatcaaaa cgatttcctg atgctttcag taaagcgaaa
    23581 agagagaata caatcaatca tttatatctt catggatcag ctgaaccttt agctataaat
    23641 gagaacatgg atattttagt agcaggtgaa gatttaaaac aagttgagat gcaaatgcgt
    23701 gatatgccta acaacagaac cctactgttt agatggatcg ttgttaacta tgaacagctt
    23761 aaagagaagt atgatctaat tctgattgat actcataatg atgcaggtct actagctcaa
    23821 aatgcttggg ctgtatctga tcttgtcgta ggcatttctg atccatctaa agatggtttt
    23881 gaagctttgc tgaagcttgg taaagatatt gatgcactag caaaacagtt gattgatatt
    23941 aagactggtg aaacactgat ggaaactaaa tacgctacga tcggtagcag gatcaaacat
    24001 aatactaatt caagtcgtga gttcctagaa ttggtaccaa cgttatctaa ttatcttgga
    24061 tatgtacaag agaaggaatt gcttaatgca actagcttag aaggtaagaa tattgctgag
    24121 ttcgctaagg ataagaatat ctatcgtaag aataaggatt tctttgaaag cacgtttacg
    24181 ctatacaaca aaataattga tacggcacaa gaataaggag tgattgaagt gaaagaaaat
    24241 agatttaaaa aattcgctga agctgaagta gaagaagttc ctgaagtggt tgaagcagtt
    24301 gaagaaccta aacaaacacc taaagaaaca acaagttctt tggtagctaa actaaaaagt
    24361 gatagttctg aaaagaagaa aaattatcaa ttcacgcttc aaccatcaca aagaaagaaa
    24421 ttatctaaaa tcagcgaaaa gttaggaatt cgatcagatt cagctttact tggtgaaatg
    24481 atagataact tgtatgatga agtttttggt gagtgatcag tttaaagctg atcacttttt
    24541 ttattggaat aagcggttag gtatagctta actaaatttc gtatacgtgt ttatggatat
    24601 atacgtatac atgtttatat acatgtatac gtatatgttt atgtttgtat ttgtacataa
    24661 taaaaaggct aggtattgta acctcgcctt tttattttta aagtaagtct ttgttacttg
    24721 gtgcattgaa cccttttagc attggctttt ctttctttgc tgaatgatcg gatgtaaccg
    24781 ttgaaggatg ctcttgttga atagggttat gatcagttgg tttcttcttt acttctgcta
    24841 ctttgctatc gtttctatcg ttgcctgctt caataattgt aacttcattg ttctttacac
    24901 gccgatatag gttcaaagct tttttgatcg tctgagagta tttaacgcct agtgtttcat
    24961 attcggattc aagaaactct gctaaaggta actcgctaac cttttctcta ttgattataa
    25021 ctacataacg ctctgatttt cccttattca tgttgatctc cttcgctgtt ttattgagtt
    25081 aaaccaaact tgtaaaagcc ttttacatta gaaaattcac tgtcttcaac aaacaaggta
    25141 tgcggaattt tatcttttac aagttgtttg tcgataatag aagctccacc accagtaaag
    25201 acaactttat ctaaggttgt gaagtttttg aatgtagcta aaacttctgc aacaaccata
    25261 ttggtgtatg tttttatttc tctttcaact acttctgaaa tatcaaaact ttcatttccg
    25321 cttggcttaa atagatatac gcctttttct ttgccttctc ggataatctt ttcaagttgg
    25381 aaagcattag gactgatcgg aaggtcaagc atatttacga tacgattata taaagtaaaa
    25441 atgccggtat ccttttgttc acgattgttt tcgtcaattt caaaattttg gacaacatca
    25501 aaaagtttcg tgccaccacc aatatcaaca aaaccgatct tctgactgat caatgtttca
    25561 tcttgtacac taccatcatc agcaagtagg aggttatata gtgttccaat cggctgtggc
    25621 agtacccaaa cggtatctac gtttatatta taagccttac cgtcaacttc aactgaatgt
    25681 tgtcgtgtgg cttgttgcat cacgtatttg acgattgttt cggtatagtc ggatgaaggc
    25741 agacctaaaa ctagattaac ggttaaaacg tcatcagatt gatcttctaa acgtgctagg
    25801 ttagctaaag agaaagatag gatatttttg taggtttcct gcttgtatct gctgatccca
    25861 aaacctagtg attctatcac gtagtcatct tttcctagct cgtgtatatc cttcccaaaa
    25921 taatagcttt cagtagtgtt tactaggcta tattcagaaa cgtttagtga tcgttttaat
    25981 gaggaaccta tattagaaaa acgtcctgaa ttaatgatat ttttgttcaa aatacgagag
    26041 ggaagtgaga gtggagaaac gtgtgtactc atttctttta ctgatttatt ccctaagtca
    26101 acggaaaaca ataatggttc tttcatggta tgacctcttt ctgtttgtgt ttatggtatt
    26161 tcggtacgaa aatagtttac acctatttac tattagttgc aactgtttat aagaaaaaaa
    26221 tgcaatcata aaagtctaat taattataat aaagtgtaaa taaaaaggtg taaataaatg
    26281 cttgcaaatt tatcttatta tgatattata aaatcataaa ttatattaag gagtgatcct
    26341 gatgcgattg agaagaaata agaattacac taccattatt ttagaagatt ttactgatca
    26401 agaattactt attgtcggta aaacaaaagc agattttatc gaaaagggaa atgcttcaat
    26461 agtaaaagct tcaaaggttg ctgtcgaaaa atattttgat tctttagaag aaagtgctaa
    26521 ggaaattttt aatggagaga aagctggtag ctatgagtta gacggtatta gatttagctt
    26581 gatcccaaca actgaaaata ttattccgaa gaaattatat tatatctttt catctgaaaa
    26641 acatacttca atgaagcctt gtatttttga ttggcaatgg ttgattgatg ctgtattgga
    26701 tgtaaaatgt tcaaaataca atattacttt ggggtgttaa ttatgagtat gctagttaga
    26761 aaattaccat tatctgtaag agaatttcta ttttacttgg tctgcttaat cgcatcaggg
    26821 tttaagctga tcgaacatat ttttatcttt gcttatgtat tagcaatgat cgcattttta
    26881 tctggcggtt ggttaattat ttatttgtta agctaagttt tagatcaaaa ataattagta
    26941 aattaaacga taaaataacc gttaataaat gtaatgaaag gacaacgcct aacaatcagc
    27001 ttgtaataag attataaaat aacataatat caaaaatagt gctttttaaa ggacaaatta
    27061 agtgtatcaa cataaacgtg tatatgtata tgtttatgtt gatacttttt ttgtcttaat
    27121 tttttatttt taaaattcta tttactaatg ctgtatagat agtgatcaga aaaaacaaaa
    27181 tagcgagatt aagctattta ttagttcata tattatattc agattaatat gatccagtat
    27241 taatctgaag gcaaaaccaa tatatttcta cggactcttg taaaactatt taaattttcc
    27301 tgctaataaa ataacgaaaa tagaatattt agctttttta taccatttta cgataacatg
    27361 atttcatttg taatattaaa acatttaaat ttttaattat tctttcaaat tagatcaatt
    27421 aatatcctta taaaagagca taataaatgg tttttacatc gttatttatt gatattaatc
    27481 tcatccaaaa aaaatgaaaa cgcatacttt tatgggtgat tgaatgtgat tttaatacat
    27541 ataccaatta aaagaaaact ttttagattt ttacttacaa atttataaaa aaacaagata
    27601 attgatactt tgattttgcg aacattttat cttgccgcct aattcagctt taatatcatg
    27661 attatatcag taacaaatcg cttttttagc taatttaagc atcaaataat tattcttaat
    27721 cagggaaaaa ataactaata aatggatttt gggaataggt aaaaacttga tcggtttctg
    27781 gctaaaaata acgtgaaaaa ggaaatataa aataacatgg agcgtgggag gaataaagaa
    27841 attttacacg aaaggtgcgc agggatgtaa agaaacttaa cacgaaacaa aagt
//
LOCUS       DANJMI010000036        27571 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000036 DANJMI010000000
VERSION     DANJMI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.036"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(308..670)
                     /locus_tag="PWU81_001885"
     CDS             complement(308..670)
                     /locus_tag="PWU81_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110581.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(744..1124)
                     /locus_tag="PWU81_001886"
     CDS             complement(744..1124)
                     /locus_tag="PWU81_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDL1110582.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1188..2381
                     /locus_tag="PWU81_001887"
     CDS             1188..2381
                     /locus_tag="PWU81_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL1110583.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2458..3042)
                     /locus_tag="PWU81_001888"
     CDS             complement(2458..3042)
                     /locus_tag="PWU81_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL1110584.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3243..4241
                     /locus_tag="PWU81_001889"
     CDS             3243..4241
                     /locus_tag="PWU81_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL1110585.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4353..4862
                     /locus_tag="PWU81_001890"
     CDS             4353..4862
                     /locus_tag="PWU81_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL1110586.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4892..5551)
                     /locus_tag="PWU81_001891"
     CDS             complement(4892..5551)
                     /locus_tag="PWU81_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDL1110587.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5553..6317)
                     /locus_tag="PWU81_001892"
     CDS             complement(5553..6317)
                     /locus_tag="PWU81_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDL1110588.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6325..7002)
                     /locus_tag="PWU81_001893"
     CDS             complement(6325..7002)
                     /locus_tag="PWU81_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDL1110589.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7039..8424)
                     /locus_tag="PWU81_001894"
     CDS             complement(7039..8424)
                     /locus_tag="PWU81_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL1110590.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8634..8718)
                     /gene="ffs"
                     /locus_tag="PWU81_001895"
     ncRNA           complement(8634..8718)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PWU81_001895"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8741..9256)
                     /gene="tadA"
                     /locus_tag="PWU81_001896"
     CDS             complement(8741..9256)
                     /gene="tadA"
                     /locus_tag="PWU81_001896"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL1110591.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9362..10219
                     /locus_tag="PWU81_001897"
     CDS             9362..10219
                     /locus_tag="PWU81_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1110592.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10300..10635)
                     /locus_tag="PWU81_001898"
     CDS             complement(10300..10635)
                     /locus_tag="PWU81_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110593.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10702..12072)
                     /locus_tag="PWU81_001899"
     CDS             complement(10702..12072)
                     /locus_tag="PWU81_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDL1110594.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12150..12686)
                     /locus_tag="PWU81_001900"
     CDS             complement(12150..12686)
                     /locus_tag="PWU81_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1110595.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12995..14476
                     /locus_tag="PWU81_001901"
     CDS             12995..14476
                     /locus_tag="PWU81_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1110596.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14579..15286)
                     /locus_tag="PWU81_001902"
     CDS             complement(14579..15286)
                     /locus_tag="PWU81_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDL1110597.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15365..15703)
                     /locus_tag="PWU81_001903"
     CDS             complement(15365..15703)
                     /locus_tag="PWU81_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDL1110598.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15814..16776)
                     /locus_tag="PWU81_001904"
     CDS             complement(15814..16776)
                     /locus_tag="PWU81_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDL1110599.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16791..18122)
                     /locus_tag="PWU81_001905"
     CDS             complement(16791..18122)
                     /locus_tag="PWU81_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL1110600.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18130..18810)
                     /locus_tag="PWU81_001906"
     CDS             complement(18130..18810)
                     /locus_tag="PWU81_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDL1110601.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19029..19508)
                     /locus_tag="PWU81_001907"
     CDS             complement(19029..19508)
                     /locus_tag="PWU81_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HDL1110602.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19635..21314)
                     /locus_tag="PWU81_001908"
     CDS             complement(19635..21314)
                     /locus_tag="PWU81_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL1110603.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21862..23364
                     /locus_tag="PWU81_001909"
     CDS             21862..23364
                     /locus_tag="PWU81_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1110604.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23377..24039
                     /locus_tag="PWU81_001910"
     CDS             23377..24039
                     /locus_tag="PWU81_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1110605.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24140..24343)
                     /locus_tag="PWU81_001911"
     CDS             complement(24140..24343)
                     /locus_tag="PWU81_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL1110606.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24454..25638)
                     /gene="iadA"
                     /locus_tag="PWU81_001912"
     CDS             complement(24454..25638)
                     /gene="iadA"
                     /locus_tag="PWU81_001912"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDL1110607.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25795..27534)
                     /gene="yfcC"
                     /locus_tag="PWU81_001913"
     CDS             complement(25795..27534)
                     /gene="yfcC"
                     /locus_tag="PWU81_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL1110608.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
BASE COUNT         8199 a         5762 c         4668 g         8942 t
ORIGIN      
        1 ttagtaacat gtcaataact attttttaaa ttttttagaa atgtgatata acatccactt
       61 cagaatcaac gctgttaaat ttatcatgta tttcttcctt ggtcaactat aaattaagca
      121 ttactttgtg aaatgatttt tcgatcaatt attattattc gttatacaac cgttttcaac
      181 atattttgaa aataaaatgc actaaaaaac tatctttagc agaacatttc attgtatttt
      241 ttggaccaaa caataaaaaa agaagtttga aaaaaattca aacttctttg ttcgcttaat
      301 ctgtatttta gaagaaaggc actccctcta cttcgtaagt tccaagaaca gagcgcattt
      361 gacctggttc ttcccaaata tatccttcgc ctaattcttc tctttgattc ttttgttcag
      421 tttcaaagac aaacaacgga acattttcct ctttaatatg tgtatttgtc aattccatca
      481 aactaatctt gctaacatcg atatgaacta tttcttttaa gaaattaagt atattcgcta
      541 aaccagtctt tccttcagaa agttccgcta acgcaaattc caacttgtca tcaattgaat
      601 ggaccaaaaa ttttttagta ccatcctcca aatgcaacat aattgtacca gcaactcttt
      661 tgcttgccaa aatttccacc tcgatcaact tgttctatgg cttacataaa aattctagca
      721 cattttcaga aaaaatacac tttttatttc ttattttctt tccgttctag ttcagataat
      781 gcttcttcta tccaaatagg taactgctcc gcaatagtgg taaatccgat cttacgattt
      841 ttattcgtga aatacctttt tctgcgatat ggaacagctg gatttgtttt ttctaacgtt
      901 cttaaagata aactgatttt tttggaatat tcatcaatgt cgatgatctg aactgttact
      961 ggctgtccga cttttaacaa tgaatggata tttttcgtat aaccggattg tacttctgaa
     1021 acatgaataa gtccttgcgt ctcgttatca agagaaacga atgcgccata aggctgtatc
     1081 cctgtcactt ttccttcaat tatttgtcca attttataag tcatctactt ccctctcttc
     1141 gcatttatca ttatactgta aatagctcaa aaaaagaagc gacgaaaatg actggaggtc
     1201 ttaagatgat cgaatttgat aaacagattt caagaaaaga tacagaaagt gtaaaatggg
     1261 acgcaattgc tgagacatat caaatggatg acttacttcc tttgtgggta gcagatatgg
     1321 acttcttgtc tcccggtggg gtagctaaag cgtttagtga atacatcaaa catggaatct
     1381 ttggttatgc cactctgtct gataaactat atcaggctat cattcattgg gaacgccaac
     1441 accatgctgt ggagttgacg aaagaaaata tcgtttttac aagtggtgtg ttaaccagtt
     1501 tagctgcagc agtacaaact tttacaaaac caggcgattc tattttgatt catgacccag
     1561 tttatcctcc attttcctct attatagaaa caaatcaacg tcacatcgtt cgaagctctt
     1621 tgctagaaaa aaataatcat ttcatcatgg acttgtctga catggagaaa aaaatcgaag
     1681 aaaacaatgt aaaagcaatg attttatgca atcctcacaa tcctggaggg cgtgtttgga
     1741 gcaaagaaga attgaaacag ttaagcgaat tgtgtttaaa acatcaagtg attctgttta
     1801 gtgacgagat ccatcaagat ttaactttat tcgatcatac atttacttcc atgctaacga
     1861 ttgatcctaa tttagatgat ctactagttg cttttacttc cgcaaccaag actttcaatc
     1921 tagctgcaat caaaaattca atggtattta tcaaaaatcc agagatgaaa gctgattttg
     1981 aaaaacattt agtgatgaac caacagcacg aaatcaacac ttttggactg attggaaccc
     2041 aagcggctta tgagacgggt ggagaatggt taagccaact gattccttat ctagaaaaaa
     2101 atgttcaaac agttaatcag ttttttgaac agtatcttcc gaaagtgaga ataatgaacc
     2161 ctgaaggaac ttacttaatg tggttagatt ttaccgccta tatttcagac gaccaagaat
     2221 tggagaacac tcttgttcgg aaaggaaaag tcgtattaaa tcctggcatc acgtttggtc
     2281 cttccggtca tgggcatatg cgcttgaata ttgcttgtcc agaagaaact ttactggaag
     2341 gacttaatcg aatcaagtat gctttagaga caatcgatta aatcatttat tcaaaataaa
     2401 aggacgagac cttactaggt ttcgtccttt ttcataatta ctccgtacca taccaaacta
     2461 ttctgaaatt tcaattgttt caatcactac atcatgtaca ggtcggtctt gtggaccacg
     2521 ttgtacattt gcgatttcat ctactgtgtc catcccttct agtacatgtc caaagactgt
     2581 atgtcggaaa tctagccaag gagttccgcc acctttatac gcttcaatga tttctgatgg
     2641 gaatccagca ccttctagtt gtccaagcat attagctggc acatttgtgt tatttacaat
     2701 aaagaattgg cttccgtttg tgtttggacc tgaatttgcc atagataaag ctccgcgaag
     2761 gttgaacagt tcacgactga attcatcttc aaaactttcg ccataaatac tttcgccacc
     2821 cattcctgtt ccagttggat cgccgccttg gatcatgaaa tcaggaataa cgcggtgaaa
     2881 aatcacgcca tcataataac cttttttgga aagttcaatg aaattttgaa ccgtcttcgg
     2941 tgctagttca gggaataatt gaacagtaat gtcaccacgg tttgttttga taacagcttt
     3001 tggtcctttt tcacttttta aatctagttg tgggaatgcc atattgtttc ctcctaatat
     3061 ttgcatactt cataataata ataacataat acctaacctc tcgtaaaaat actagatttg
     3121 ttttctttta tttttcatca ctagatacat tctataactt accgtttcga tttcgaattc
     3181 aaacattttt gagaagcatt ctcaattaaa ttctgatata gtgtgaaaag ggaggaattt
     3241 ttatggaaat ctatgacatt acaatcatcg gtgcaggtcc tgtagggatg tttgccgctt
     3301 tttacgcagg aatgcgccaa gctaaaacaa aaatcattga cagtctacct caacttggtg
     3361 gtcaattagc aactttgtat cccgaaaaat atatttacga tattcctggg tatccagcaa
     3421 taaaagcaag tgaattaatc gatcaattag aaaaacaatt aactactttt aaccatacat
     3481 tccatttaaa agaagaagtt ctctcgctta ctcgtgaaga cgaagttatc gagattacta
     3541 caaataaagg catccattat tccaaagcag tcattttagc tttaggaaat ggttcattcc
     3601 agccaagaaa attgaattta gataatgctg aatcatttga aaaccacgga ttggattatt
     3661 tcgtgaatga tttgatgaag tatgctggaa aaaaagtcgc tattgctgga ggtggtgatt
     3721 ctgctatcga ttggacgttg atgttagagc ctattgcaag cgaagtttac ttgattcatc
     3781 gtcgcccgga atttcgtgcg cacgaacata gtgtatcacg cttgaaatct tcatctgtca
     3841 acttgcttac tccctatctc atcgatggat tatcaggcaa taatggggaa ctgacagata
     3901 tccggttaaa aaaagtgaaa agtgacgaga cgattgattt gatgattgat tctctgatcg
     3961 tcaattatgg attctcttct aatttagaac atctttcttc ttggggactt gatagtacca
     4021 gaaatgccat tactgtaaaa tcagatatgt ctacttctat tccaggcgtt tatgcagctg
     4081 gtgacatctg tacctatgaa ggaaaagtga aactgattgc tactgggctc ggtgaagcac
     4141 ctactgcagt gaacaacgct ttacactata ttaatccaaa ggaacgaact cagcctggtc
     4201 acagcacaag cttatacgat aaaaatcttc gtcctagttg attttttata tctctttgca
     4261 tttcaagtta aacccgaaag cagtttcttg catttttttc atgttccgtt aacatgaaat
     4321 agagaaagaa aaaataaagg gggagcaata atatggtcgt caaaactgag actttagaag
     4381 aaaaggcaag agaactattg aaggaacgtg gcgtaactgt agaagatatc gcacaacttg
     4441 tcatgttttt acaaaaagac tatatttctg atttgacttt ggcggattgt actgaaagtg
     4501 tcaacagcgt gctaacaaag cgtgaagtac ataacgccat tatcactgga atccaattag
     4561 atatattagc agaaaagaaa aaattgatga gtccgttgca acatattatc gaagatgacg
     4621 aaggccttta cggtatcgat gagatcctag ccttgtcaat cgtgaatgtt tacggtacga
     4681 tcggctttac gaattatggt tatatcgaca aggtgaaacc tggtatttta aaagaactaa
     4741 acagccacga tggcgttcat gtccatacct tcttagatga tatcgtcgga gcgatcgccg
     4801 ctgctgctgc cagtcgatta gcacacaaag aaccagaaaa aagaagtcgc cttactcaat
     4861 aattcaacaa aaaaagaaag aaaacgtcta tttagacgag ttctttcttt tttgtttgtt
     4921 tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat aatacaaaaa
     4981 ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt ggatcagtaa
     5041 cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg ataaaaaagc
     5101 gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg ccgatttgaa
     5161 acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata aacaaaatac
     5221 tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt ttcacttcat
     5281 aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg atggttttga
     5341 agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc tcttgatcta
     5401 aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt ggtcgaaagt
     5461 tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac aatccagcat
     5521 attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc cagtgattgt
     5581 ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt aaaacctgat
     5641 aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt ttcatagtta
     5701 tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc tttatccata
     5761 atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt ctctacaaaa
     5821 gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt cttatctggg
     5881 ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac tactttttca
     5941 taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc ttcccatgta
     6001 aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata gacttttttc
     6061 ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta gactgtctct
     6121 ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc tggtgactta
     6181 gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc ttccacaaat
     6241 cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa atcaattaaa
     6301 tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa atgctgtttt
     6361 tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg tttttgctga
     6421 ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg gtcctttttt
     6481 ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag ttgtcttttc
     6541 ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc aatattcgta
     6601 aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat tatccgcttg
     6661 gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat aatcatatcg
     6721 ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt cacgataatc
     6781 ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta acagtgtcac
     6841 tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca aagcagctgt
     6901 cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag caatttctgt
     6961 tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac cacctatctt
     7021 ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat ctcctaccac
     7081 acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa tcgttggaaa
     7141 aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat tcagatcgat
     7201 tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc cattatagac
     7261 catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt tacgtaaaaa
     7321 gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac ccaatgtgac
     7381 atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat tgacccgccc
     7441 ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg tctttcctcg
     7501 ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga tatctagact
     7561 cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc cacgttctag
     7621 acaactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg tagggatggc
     7681 tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga cttcaccgac
     7741 atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt gaccttctct
     7801 gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt cttttgcaat
     7861 ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca cttgtgaacg
     7921 taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc agccattcgg
     7981 attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg tctggtgttt
     8041 cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat ttttatcgaa
     8101 taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca actcttcttt
     8161 ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa cttcattcat
     8221 cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac gatccacaaa
     8281 atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt ctcgtttacg
     8341 ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca gatcattcgt
     8401 atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt atcataccat
     8461 aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg ccttgaagca
     8521 aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga acattcattt
     8581 ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt tcattataat
     8641 cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac gcttcacaag
     8701 cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca ttcgttgacc
     8761 agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca gtcaaaatca
     8821 tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta aaccgctcgt
     8881 cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac gcgccgaaat
     8941 aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa ggctctaagg
     9001 taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag gcttccttga
     9061 ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg ttatatcctc
     9121 ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca tgaagcattt
     9181 ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct actgcatcaa
     9241 gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta ctttagcata
     9301 tgataaaaag gctgtaacta actttttttc tatggtaaaa taaatacaag aggtgaacaa
     9361 gatgaaaatg gataaattac ttgctcttta cccacaagca cagaaaaaaa actctccagc
     9421 atcagaagcg gatatccttt cttttgcaat tgatgatgct tttatctgga tcaaacaaga
     9481 ttcattatct caacaagaaa ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca
     9541 aaaaaaacac ccatggtatc actatctttt taaagatgct tcttgtgttc aagaaaaaag
     9601 ctttcgtttg atccagcttc acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca
     9661 agatactatt ttggaattat ttcgctattc tgctgatttc tttttttata cagacacaga
     9721 tgcgctctta gtcgaacaaa taaaccgtga ttatcttgat gctactgaaa tcaacggtat
     9781 ttttctatct ttagatgctg attttgatac gacaacgtct gtattcgttg gttctttcca
     9841 ttcacctggc accgacctcg tccctttgtt tgctgaagaa agaaaaattt ttttaacaga
     9901 gcaaaacaat ctttatactc acagccgtac tttttctatg caagatgtgg ctttacatta
     9961 ctatacaaaa gatactgtaa aagatagtaa gttgatcaaa agttacatag aactgatcaa
    10021 acaaaatgaa atggaagtga tcattcaaga attatggaat gaattaggca atgtcagctc
    10081 tgctgcaaaa acactgtttc ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca
    10141 agaacagtct ggtttcaatc taaaaaaagc caacgattta ctgttttgct atcttttatt
    10201 gcttcatgaa cataattaaa gatagattaa aggggttgtg acaataattt tgtcacaacc
    10261 ccttatttcg aatgaatagt gttcaaaagc tagtttctct tacatcattt tttcaacaaa
    10321 atgcattagc cggtctttta catcttcggt tgaatctttg attgaatcac cataaacaga
    10381 gattttcttt tttcctgatt tgatttttgc taacattccc atcaaagaat ccaagtcact
    10441 atctgaaaga ccatctacga tttctagtaa tttttcattt ccatcaaatt tgtcatagac
    10501 gaattttttt actttcgtcc gagtagtcat atgatggatc ttttccatta cttttcccga
    10561 tacaacgact gctgtcgcta cactggcagc ggctgctacg cttaatccga ttgttacttt
    10621 tgttgatgtt ttcataagta tcagctcctt tttatctatc tccatcatag catagacaaa
    10681 aaaataaacg aactaatcca ctcagtgaaa tctcaatcct ttagggaaaa agttctttac
    10741 tgttcctttt acttgtttcc caaatccgac aggtatctta tctaatacta gcaaaaccca
    10801 tccttgattc ccatcacgtt gaaatgtttc tcctgctgtg taagattgcc attccttttg
    10861 agtgatcggc aagcaaggta tattctctat cttttttgta gcaagcgcta atgcatagct
    10921 aggttcaaat cgattctttt tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa
    10981 accatcgaga gaaggggcta actcaggaac ttcccaaaga tggtcgttga acactaaaag
    11041 tcttccagta gcttcataat gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc
    11101 ttttgtcatt tgcactttac ttttttttcg tgtcttcttt tctttatgca tctgattttg
    11161 tccatgaaaa gtcagctttg caacaaaatg tccttcaccc tgatctttat gaggccatat
    11221 acgtattgtt ttttccaaac cagctacgct tccccattca gaacgtcctg aactaacgga
    11281 ttgagtcaaa ggaatctcct caatcgttac tggataattt tctacaagcc agctaatgat
    11341 ctcttcattt tcttctggtg caaacgtaca agtcgaataa atcagttgtc ctttattttt
    11401 tagcattttt attgctgaag acaaaatctc ttgctgacgt ttttgacagt atagcggaga
    11461 ttcttctgtc cactctttta ttgcatttgg atcttttcgg aacattcctt ctcctgaaca
    11521 aggagcatca accacgatcc gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg
    11581 tgcatgattt gtcactattg cgttcgaaac tccccatcgt tctatatttt ctgataaaat
    11641 cttcgcacgt ttcggaaaga tctcattcgt cactaataac cctttcccct tcatttgagc
    11701 agctaattgg gttgatttac caccgggagc cgcacacaaa tccaaaactt tttcaccggg
    11761 ttttgctgca gcagctgttc caacgatcat tgcacttggt tcttggctat attcataccc
    11821 agcttgatgg aggaaagact ttccattgac agtgccaaga aatccctcat tgctgtaggg
    11881 agcgggttgt aattcttcgc tatggtaagt ttggaccatg tccaatcctg ccggttttag
    11941 tggattccac cgaaatcctt ttttcacgct tccttgttca agcgcagaaa aaaaatcgga
    12001 tgcttcttcg cctaaaagaa gacgatattt tttgataaat tgctgtggta acgttgtcgc
    12061 ttcctccttc attccaatgt ttcgttagac attttacaat cttttttacg aaaaataaag
    12121 gtggctgatc tactttttca tgttgctgcc taaggtgact gttcctttaa gatattttcc
    12181 aagtattcat ccgtcacctc gtttttatcc caaacagcgt tcatcatttc aagaaaatct
    12241 tgagaagaat catctgagcg caaacttccc aatgcattaa gccataccaa ttcaagcgac
    12301 ttttcttctt tgatcttcgt gtgacctttg acatatgaga aaaatggccg cttaaatagc
    12361 gaatccatgt ctaaatcaga ttttgcaatg atttcacgaa tactttcatg gatagaaaaa
    12421 aaatctttca ataagtagac aatgcttaaa acatcatcgg aactgcattc agctaagtag
    12481 ttttcaaaat aaacagtgat ttcttctaat atcttctctt ttaacccgct gattgatttg
    12541 aaatgtgtat aaatcgggta tgtagaacaa ccgcagaact ctgctaactc tcttgcagta
    12601 attgacttca ttccatattc ttccacgtat accatacttt tgaacagcaa atattcttta
    12661 ctataatttg ttactctccc catcattctt acctccgtct cttttcatct acccataaat
    12721 aaataacata aataattcta aaaatattat tgataattat caaaacatat ttagttatat
    12781 ccattaaaaa tcaatttttc cgccttaaaa acaagaatat cttgacaaaa taccctagga
    12841 aataaacaat agaataaaca aggtaacaaa aaaattaaat aacgttatat aaaaaagaag
    12901 gcgacactct gtgagaataa cacagactga aatgactttc cactcacagt ttttgtctaa
    12961 ctatatacaa aaacagaata aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga
    13021 gttacttgtt ctacaaatgg tcattcaaaa aaataattgt tctttacaag aattatccaa
    13081 tgacttacat attccaaaac ggacaatcaa agaactgatc cgaaaaataa atgtatcact
    13141 caagcaattt ttattgattg atgaatttat ttattctaat cataaaggag agatccaaac
    13201 caaagaaacc gatcaaacta aaaagttagc tgttttttct aagttaaaat tatgctacct
    13261 aaaagaatcc aatcgtttta actatttgat gttactaatc aattatccac aaactgctgt
    13321 accaaaaaaa tatttacttg aacaacttta tatatcgccc tcctatctcg aaaaactgac
    13381 aaaacagctc aatcattcct taataaaatt ccatttacgg atcctatctg taaaaaattg
    13441 ctgtactctt gaaggaaatg agttatctat ccgtctctat ctttactttt ttctttcaga
    13501 tacatttgga ggtaacgatt ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa
    13561 cagtacagat gagcaaatag aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt
    13621 aactttattc atattacgaa acaaacaaca ggcatatttt ccaaaaactt cttctgaaat
    13681 cactgaattg atggacttat tgcagcatac acatgattat tcagaatact ttacttcttt
    13741 ttcagtgagt cgcccttgtg aagagacaga aacagagaga gcctacttta attatttttt
    13801 ctattgtttt tttccaaatc aaatctcttt tcgacaaaaa caggagattg gttttttatt
    13861 ttccacaagc aaaaacctta tttgtcgaca agtcggtcaa ttgatgactt acttaactca
    13921 acagtttcct ctcaaccatt tagaaaataa aataccaatt tatttttatt ttcttgtgtt
    13981 ggcagttgtt tttttcgaac tgttgggtga atccagcagt agtttttcta cactttgctt
    14041 tccagaagcc acgtccaatc attctagttc ttttatacag gaaaagaaat taagagaaaa
    14101 aatcgataca attttttcaa aaaatgggga aagaaaccaa atcagtcaat accttagtca
    14161 attgatccaa cacttactac aaacagaaac gaaagagcag ttactcatcc atttgcagat
    14221 aaccaaacag tttacaggaa attatcatct tcaaagccat ttgcagcagt tatttcgcaa
    14281 agatgctgtt aaaattaccg catattcaga acatgctgat ttgatcatta cagatcagca
    14341 tgagaaaatt tcagatgaaa cggagacttt ctatctggat acaacgcaca atacagtgtt
    14401 atttggcaag ttaataaaag cggttcatga actgtatatg aaaaaaggtt atgagaacaa
    14461 aacaaagagg atgtgacaaa attcttgtca catcctctta ttccaaataa atggtattca
    14521 atcagctaac tctcggtatt agctcagatc aaagcgctga tttcacaact tctttatttt
    14581 aatctcttcg gttgccgccg aacaataaga tcaagcgcaa cagctgcagg aatgtcgaaa
    14641 gcgctgctgc aacataagtc agtgctgctg caaataatac gtgtcttgcc atcggtactt
    14701 cttcttccgt cagtagtccg ccttcactca aaatttggat tgctcgccga gacgcgttaa
    14761 attcgactgg taatgtcacc aattggaaaa gtaatgctaa agaaaaagcg aaaatcccga
    14821 tattgattaa cgtagagttc cagctaagca atacacctac cataatcaag gggaacgaaa
    14881 tcatcgaacc aaagttagct gctggaacaa gtgctgcacg aatcttcaat gggacataac
    14941 ccgaagcatc ttgtaccgca tgtccgcatt catgagctgc tacacctatc gcagcaactg
    15001 aagtagactg tgcagttgct tgagacaaac tcaacatctt attacctgaa ttgtagttat
    15061 ctgtgagatc tccggcaatt tgttgaacac cgacattatt tatctgttga ctttgtaata
    15121 tatattgtgc tgcttgtgta ccagtcacat tgtttttgct tctcacttga tcgtattttc
    15181 ggaaagtact attgacgtac gctgacgcaa ttccggaaat aactaatcca agaatgacca
    15241 aaagaaatgt cggatctaaa aaaccataca ttccataacc atacattttc gtttcactcc
    15301 ttttcgttta ctttatttta tcattagtag taggaaaaga atgtgaaaag accttgaata
    15361 ttctttaatt ctttttcaca aattccgatt tcagtttcat cggtccaaat ccttctacct
    15421 tgcaatcgat attatgatcg ccttctacta aacggatgtt cttgactttt gttccttgtt
    15481 taatggcacc tgacgcacct tttactttca agtctttaat cactgtcacg ctatcgccct
    15541 ctaacagacg gtttccattt gcatctaata ccactaatcc gtcttctatg acagtttctt
    15601 gttcttcttt gttccattcg ttcccgcatt ctgggcaaat caacaagcct cgatcttcat
    15661 atgtgtactc tgagccacat tctgggcaat ttggtaatgt catcttcttt ctcctttaaa
    15721 atctttagtg agcactattg taacggaaaa agaagatctt gaaaagatac atttgtgatt
    15781 tttactacaa aaggcacgtt ttcgaacgaa gtatcattct gtttttctgc tcttatacga
    15841 ttggcatata tcttggatct ctttatagat ttcctctact tctgctaaat cttttgcatt
    15901 cgcaccactt gctaatggat ggcctcctcc atgatggcgt ttagccaatt cattgatgat
    15961 cggcccctta gaacgtaaac gtacgcggta ataccctgaa ggctgctgaa caaaaatccc
    16021 ccaagccagc acttcgtcaa tcactccagg aagtgaaacg atcgaagctg tctctgaatc
    16081 atcgattcca tatttatcaa gaatttcttg cggaaggatt attcttcctg caccattttc
    16141 atccacctgg atattttgat aaagataacc ggaaagttta gcgactttca tcggcatttg
    16201 ttcaatctct ctgttcaaag cacttgcatc aaatggataa tttctcaata tcgaagcaat
    16261 ctctaatgta cgggacgtcg tggagggata caaaaagcgc cctgtatctc cgacgattcc
    16321 tgcataaagt aaccgtgcag ccttaacgtt catctttaaa gattcaggaa acatcaaagc
    16381 aaattctgca atcatctcac tacagctact tgcttttgta tttacccaca caagatctcc
    16441 ataagggtca tcattgggat gatggtcgat tttgatcagt ttttctgcca actgatatct
    16501 ttgatcactg attctgggtg aattagctgt atcagtcacg atgactagcg ctccttggta
    16561 gaagacatct tcaacttctt gcatttctgc taaaaagcga agcccttcaa tcgtttcacc
    16621 tacttgacgt atttcttttt ctgggaaact ttcccgtaat agttcggcta atcctacttg
    16681 tgatcctaat gcatcaggat cagggcgcaa atgcctgtgg ataatgattt tatcgtattt
    16741 ttttattgta tctactattt cttgctgtac tgtcatttca ctcctgcttt ctaagaacgt
    16801 tccattactt gacaaacaac gattgctttg gaaacgatgg tgttttcaat aaacacttcg
    16861 atatccagtt tagcggaacg tctgccgatt tccaatacac gtggacgaat gtctaattcg
    16921 ctttccaatt ggatcagccg caaataatgt aggttcactt gttcaatcaa gatatttcgt
    16981 ttttggttca tcaccattgt tttttgtgcg acatcagaaa tgatttcgct aagtacgcca
    17041 aaagaaatgg ttccaacgcc attgaccatt tgtggtgcta ctacgaattt gaacttaggg
    17101 ttttccaaac gatttccttc acggtcagta tcaatcgtat ggatatttcc agatatctga
    17161 tccgaaatcg tatctgcaat ctgcggttgt cgctggacaa gctgcatcgc tttcattaca
    17221 tcttgccggg taatcaagcc ttccaatgtc aagtcatcgg aaacgaccgg catgacttct
    17281 agcccatccc agatcatttg atggctaaca gaggcaatac tcattgtttt ctttacactg
    17341 attggttctt tcgtcatcac ccgatcgatg atctggtttg gcgatttccc taaaacatct
    17401 tttgccgtaa caatccccat caaacgatga tggcggttga cgactggaaa acgcgaatgc
    17461 tgcgtttctt ctgacaaacg ctgataatca gaaatcgtat ctgtctgctg caaataatga
    17521 gtcttttcag ctggcatata gatatcactg acaagcatga tatctttttt gatcaattgg
    17581 tcgcttaacg ctcggttgat catggttgcg actgtaaatg tatcataaga ggtgcgtaag
    17641 atcggcattt caagatcatc agctagcttt gcaatctcag cagtcgtatt gaatcctcct
    17701 gtgatcaata ctgcggcgcc atcttccaat gctaattttt ggacttccgc ccggttgccg
    17761 acgatcatta gtgatcctgg cgtaatatag cgttccatgg catgtttagt catcgcgccg
    17821 atgacgaatt tattcaagac cttgtctaat cctgccgccc cgcctaaaac gtccccttca
    17881 atgatcctga cgacctcgcc aaatgtcaaa cgttcaatat gtttttttaa tttgcgttcg
    17941 atacggatgg tccctactcg ttgaattgtt gaaactagtc cgacattttc tgcatctttg
    18001 attgcacggt acgctgttcc ttcactgaca ttcaattctt ttgcgatgct tcttacagaa
    18061 attcgattgc caacaggtaa actttcaata tattctaaaa tcaaatcatg ttttgtcgcc
    18121 attttctgcc tacacttcca gcgtttccga gacgctcatt atttttcctt cactatcttc
    18181 cagtaactct acaaaagatt cgggatcttg ttcaatgaca gggaaagtat tgtaatggat
    18241 tggaactact ttcttggcac gaattgtttg cgcagccttt gctgcatctt ctggccccat
    18301 cgtatagtta tcgccaatcg gcaagaaagc gacatcaata tcgaattctt ctgcaagcaa
    18361 tcctagatca ctgaaataag cagtatcccc tgcatgataa atcgtttttc cttctgcttg
    18421 aataataatt ccgctaggct cgcccatata ctgaacgata ccgtctactt catagcttga
    18481 actatgctgt gcatgtacga attttaccac gccgaatgga aacgcatgtt ttccaccgat
    18541 attcataccg aatgaatcta ctcctctgct gttcgcataa tcagcaatct caacaatact
    18601 gataattgtg gcttgatttt cttttgcaat aggaatcata tctcctatgt ggtcactatg
    18661 accatgagta atcagcaacc aatctgtctt tacttcttct gcagttaagt ctgtcaatgg
    18721 atttcctgta ataaacgggt caatcatcac gcgttggcca ttttccattt caattgttaa
    18781 acaggaatgt ccatgatacg taattttcat attttacacc tcgcgttttc tctgtatctt
    18841 ttttattaaa aacgatttca tcacttgcac atcaatgaaa aaatatagct gctaaatata
    18901 ttttcattgg attgatagta cagtatgtac aaagcaattg taacagataa tacaggatgc
    18961 accaagtacc aagcagatat aatctcaagt ttgaggtctc tgctctgctt agcttctctt
    19021 ctcgtccctt aactaattaa atcagcgtaa ataggatgga cgatcttttc taaatcggct
    19081 acttttatcc gaaggcttct cccgatttca ccgcttgaca caattacttc ttctttttct
    19141 tttaaagaac tgtctgctaa aaaacaataa tcaggatgtg cgagttttat tccaataggg
    19201 gtgtttgctc cgtggggata tcctgtatgc attaatacaa aatccattcc aggaaaacca
    19261 actttacgat ctttcgtcac ttgtctcgtt tttttgtaat ctaagtgatc gttcaaagga
    19321 atcaatgcga tgatcacccc agttttgttt cctttcacta cgagagtttt acatatcaaa
    19381 gctggatcaa tccctctttt ttgtagttct tctgtgaaag attcttctac cacatgacta
    19441 aaatcaagtg gttgatagct gatcttttgt tcttctaaat aacgttcgac ttttgtttga
    19501 ctcatcattg attcactccc tttattctat cgtttattat aaagcgaggg atgctgtatt
    19561 caaaaattat ttgtttttcg caaaaaaaga gcaagacgag cactctatag agaggttcat
    19621 cttactcttt ttgtttattt tttatttcga cgttgagtag cttccataat gactggcaag
    19681 atcactgggc ggcgttttgt ttgttcaaat aagtaacggc ctaattgatc tcggatgtct
    19741 tgctttaatt tgctccactc aaaatcattg ctttctaaat gtttctcaac gattgtttca
    19801 atcatattgc tgctttcttt catcagatca cggctagctt ttacgtaaac gaatccccgt
    19861 gaagtaattt gaggttttga aatgattttc ttttctcgcc gactgatagt gacaaccgct
    19921 acgaaaatcc cgtcttctga caagattttt cgatcacgta aaacaatgtt tccgatatca
    19981 cctatgccta atccatcaat cattacattt tctgcacttg tagttcctgc aacactcata
    20041 cgcttgttct tatattccag cacgtctccg cgtccagtaa tatagatatt tttgtatggc
    20101 atccctactg catgagccaa atctgcatga gcagctaatt cacgatactc tccttgaact
    20161 ggcacaaaat atgttggttt catcaaattg atcattaatt gcaagtcatt aggattggca
    20221 tggccagaaa cgcgcatatt ctcagagatc aacttcactg ttccaccagc tcgataaatg
    20281 atatcttccg ttttcgccac gatggtttcc atcgcgatgc ttggcgtagt agtgatataa
    20341 accaaatctc cttcttcaat cttgacatta cggtgtgtac cgtttgccat tttttgcaga
    20401 gacttgatgg gttcacccat tcggccagtt tctaaaatga tcagttcttc tggatcatag
    20461 tttttcatgt ctttcaacgt aacaagcaga tcttcacttg gtaattgaag tttttctaaa
    20521 cgcatcgctg tgcgaataat tcgttcgaaa tcttgaccag tcaagacaac ttttcgttgt
    20581 gctttttctg ctgcatccaa tacttgttgc actcgttgca aattacttgc tacacaagcg
    20641 acgatgatac gcccttccca atatttgatc gtatcaaata cttcatctgc tatttgggct
    20701 tcagaagcta caggctgagg attttctgca tttgccgatt cacttagtaa ggctaagact
    20761 ccttctttgc cgatttccgc caatcgtgca taatctgttt gatacattgg gatagccgtt
    20821 tggtcaaatt taaaatctcc cgtataaacg atactacctt cttttgtttt cagattgatc
    20881 cccactgaat ctggtatcgt atgagtggta cggaaaaagc tcactactgt ttcaccaaag
    20941 tctatttctg tatgctcatc taccacatgg aaatccttaa atcctttggc tgcttcatta
    21001 cgactgacat tcaattttgc taactcgatc gtcaactctg tcccgaatac tggtacagaa
    21061 atttttgaca acaagtaagg aagtgccccg atcgcatctg catgtccatg tgttaaaaag
    21121 acaccagcta cacgatcaat gttttcttct aaatacgtaa aatcagggat tacgacgtcg
    21181 atccccaaca actcattttc aggatatttt agcccacaat ctaaaataaa aatatcctcg
    21241 cccacttctg cgacatacat gttttttcca ttttcacgaa cgccgcctaa tgggataatt
    21301 ttaatggtac tcacaaactt cacctcttct tatgacatcc aagtttaaag atgtcgattt
    21361 tcttctttat ctaaatatat gagccattcc aatgaagcat atgcaaaatc actatttctc
    21421 aacttatgga gaatcagcag gaaaattacc agcacatccg cgttctgaca gttggatggg
    21481 cattttattt tggatatact tcattaacaa aatggatctt ttgcgaaacg ctatagccag
    21541 aatacttata taagtattca gctgttcccc attattatac aacaaataaa ctaaatcgct
    21601 agtatcagca attgttagaa caactatgca cgtaaaaatc cctctgtagc atctaggatt
    21661 tcttttgcta ttcctctttc atttcttctt cgtcggtttg aaacatactt ttttcatttt
    21721 accacatttt ttgcaataac tatagtgact ttttatatat acagaacctc atgttgatgg
    21781 tgtgcaaaca gcataagatt taagctagct ttaagatagg ttgtctatta tcaaatgcat
    21841 ctactaggag ggatgcaaaa tatgaatttt atgaaacgtg catggaaaag tacaaaagta
    21901 aaatggggac gttcattgtt attgtttgcc gtgttcacag ccattcttat ttttgtgcta
    21961 gctggcctca cgattcgaag cgctgcagaa gaagcagcga atcaagcaca aaaagaagtt
    22021 ggagcaacag tgactttaag tgccaatcga gaagcttcat ttaaaaaaca ggaacaaact
    22081 tcttcaagcg gttctaccac tgaagaacgt ccagatccag gcagtttttc tcttacacca
    22141 gtttccttaa cggacgcgga aaaaatagct gaattagata atgtaaagag ttattccttt
    22201 gaaagttccg cctcagctat ggcaaaagat ggaataactg ccatcactag ttctgattca
    22261 agtgaagaca gttcagaaac tactgatgca ggcggtgaac ctgctggagg aatgggcggg
    22321 aatccgcaaa tgactcaagc agactttcaa atcagtggtg tgtcagcaac tgcacaaaat
    22381 agtaattttt ctgatggaac agcaaaaatc acggatggtg aaggaataga tgacagcgac
    22441 aaaggcacaa acaatgtagt cattgattct actctagcag aaacaaatga cctggcagtc
    22501 ggagatactt tcacaatcac gagtactgaa gatgaagata ccacttatga aatgacgatc
    22561 aaaggaattt atgaatccag cgaaacaagc ggcagtatgg gtatgaaatt caacttcatg
    22621 aatccgtcga atacacttta cacttcttac acttttgcaa atgaactaaa tgggacatct
    22681 gacgataaca cgatagattc tgctatttac actctatctg atccagataa aatggatgag
    22741 tttctcaaag aagctgagaa gctgatcgac acagatacat ttagcttaca atcaaatgat
    22801 tccatgtatc agtcaatgtt ggaaccttta aacaacgtgg caagtttttc taaaaatgtt
    22861 gttttactag tggctgtagc cggtattatc atacttactc tgatcattat gattaccatt
    22921 cgggagcgtc ggcatgagtt aggcgtcttg ctatctttag gagaatccag aagtaaaatc
    22981 attctccaat tgtttaccga agtggctatt tgtatgattc ttgcattagg agttgcttcg
    23041 cttagcggaa acgtcgtcgc gaatgccgtg ggacagcagc tacttgacca acagacagaa
    23101 accacgacac aaaaccagca agctcctggc ggtggacaaa tgccaggtaa cagacaagaa
    23161 caaggcggtc aacgcgatgg tcaacaagga accacgaata atccatttgc tgtttctgat
    23221 caagtcagcg aattggaaat caatgtcaaa cctgcacaat tgggactatt agccggagta
    23281 gcatttggaa tcagtctgtt ttctgtcttt ttagcttcta tagggatatt aagattaaac
    23341 ccaagaaaaa tcctattaaa ctaaaggagg aatttgatgt tacggacaga acaattggga
    23401 ttttggtatc aagatccgca agatgcgctt tataaagatg tagaactgaa tttcgaagct
    23461 ggaaaaatgt acgctatttt aggagcaagt ggttctggaa aaacgacttt tctttcgctt
    23521 atttctggac tagatgcacc aaaagaagga aaggtatact atcaagatcg ttcactgaac
    23581 aaaatcgggt taagaagtta tcgtaaaaat gatgtctcga ttattttcca agcttacaac
    23641 ttgcttcctt atttatcggc tctagacaac gtcatagcag caatggaaat cactaagacc
    23701 aagcaagaga attatcgaca agttgcttta gatcgactgg ctcgtgtagg aattgatgaa
    23761 atattagcaa aaaaacaggt gaccaaactt tcgggaggtc agcagcagcg agtcgctatc
    23821 gttcgtgcta tctgctgtga acatcaacta atcgtagcgg atgagcctac tggaaatcta
    23881 gatgaaacaa cttcacaaga tatcgtcaaa cttttccaag aaatcgccca tgaacaaaat
    23941 cgctgtatta tcatcgtgac tcatgagatt gaagtagcaa aaagctgtga tgaagtcttt
    24001 gaactaaaaa ataaaaaatt tcaaaaaatc gaagtttaaa aaacaggata atgatcaaat
    24061 aaaaaatccg aaccatatct gtctcgcaga tatggttcgg attttttatt tgcttgattc
    24121 aattagtaat aagtggtttt tatttttttt cgtcttttcg agaaaacttg tctttgactt
    24181 cgtcgcctaa atttcgagct ttttctttga tgtcagcacc tagtgattgt gctttccctt
    24241 ctgtttcttt gctcttgtcg tctgttgctt tgccgtatgc ttcttttcct tcaccgacta
    24301 ctttatcttt catatcttta gaacgtcctt ttaaatctgc cattgtgctt cctcctctta
    24361 gtttgtttat tgatctacct tcagggtaac atcaacaaca gatacagaca aacagaacgc
    24421 tcgttttcta aaaaagatag ttagagctgt tgattaaaaa cttttttcga aagtccccca
    24481 tactttgact tctttttctt ggaccattat ttctccttta gcgataactg tatcaatatc
    24541 aagcgtttct ttatccaaga tacacagatc tgcatcagca ccaatttcga tttttccttt
    24601 tttatccaat ttcaatatac gtgctggatt actggttata gctcgtaagg cgatttccaa
    24661 agggatactt tctttttgca cagcttcttt gatccctact agcaatgcct tggcacttcc
    24721 tactcctaac cctaggaaat gattgttttc atcaaaataa ggcaagcttc cttgtccatc
    24781 tgaggtcatc gtgaaattat ctaaagaaac atcttcctcg atcaatcgtt tcagtgcttt
    24841 gctgaagcga acttcaccat ctgtcttctc ccaaaaatca ggatcttcgc tagcagtgaa
    24901 gtcaatcgtg ccaccacgtt tggcaaatgc cacgcaagct tcaaataatt ctggtgtgcg
    24961 atttgcatga gtaggcaaaa attgtgtgat cggaatttct gtttcttcta cgattcgtgt
    25021 caatagctct agctttcttt ttcctccgcc taagtggaca ttgatgatcc cagcttttcc
    25081 ggataacatg cctcctacac gagcatctgc tactgcacga gcgaactctt caaaacttgg
    25141 ctgagaagaa cggtgatcgg atactgcgat ttctccgatt ccgataattt tatcaattgt
    25201 caaaaagtct ttgatgatag aatctgttac tgtttttacc ggcaatcggt acgaaccttc
    25261 gtatacataa gtggagattc cttctgcttc cagacctttc gctttgctga tcaacgcagt
    25321 catatctcta ccttctccgt ctgttcccaa acaaccgaca actgtcgtta caccagctgt
    25381 agtcaattgg ctcaatgtga cttctggcgt tcgattttga taaccgcctt ctcctcctcc
    25441 acctaaaata tggaaatgac agtcaatgaa acccggggtt agtacttttc cctcacctga
    25501 aagttcttct acttccactc cttcgtagcc gccttctagc tgttcttcta cagccaaaat
    25561 cttcccaccg gcaatcagta catccatttt tcctaagtaa tctggtgcaa acacttcaat
    25621 ttgtcgaatg attttcatac atttttcctc tttctttaag tataactgat gataggatct
    25681 taaaaaataa tccaaacgat aggatcttta ctcctttgat tttaccgtaa aatgacggtt
    25741 cttggagtcc ttatctactg tttggattac ttttttattt acaggtctcc agtactactg
    25801 cagacctatt gcaatggcaa tcatgatgaa aatagttcct aaaacaaaca ggaatccttg
    25861 catcttgatt tggaatcttg cccattttgc ccattcgatc cgtgcaacac ctagcacacc
    25921 aatcaagctg gctgatacgg gtgtaaaagc gtccataaaa cctgaaccaa gctgataagc
    25981 aagaactgct acttgtctgg atacacctac taaatctgcc agtggagcca tgattggcat
    26041 agtcaaagct gcttgtcctg aatttgatgt aacaaccaag ttgaacaaac tttggaagat
    26101 gtacatcgcc caagcaccaa tgacagctgg aacaccggtc aacgccgtcc cgatcccatg
    26161 caaaatcgta ttcaaagctg aaggaacaga tgcatcagat cctcccaaga caagaagaat
    26221 ccctttggcc atacctacta cgattgctgt accagctaaa tcagctgcac cagattggaa
    26281 agaagaagcg atgtcattga ctttcattcc atctaatttg aaaagaacag caatgattcc
    26341 agcaactaat cccatcacaa agaattgaga agcgatttct ggaatgtaat agcctttttg
    26401 agtcacgccc caaacgatcc agacaaggac aactaacatc tctaataaga tcaatttatg
    26461 cccaagcatg aattttttat tttcatcaga tgtctttttg atatgagaac ggaaataatc
    26521 atctgatttg taagtcaacg aactttctgg ttttttacgg attttttctg catatatcat
    26581 catataacca gcggctaatg cagtaacaac tacccacata atcaatcgga atgtcgcacc
    26641 agacaacacg gggattccag caatcccttg agccaccgct acgctaaacg ggctcatcca
    26701 agaggtcgca tttccgactt gagatgcgac ataagtcacg gttactgcta cgatcgagtc
    26761 atatcctaac gcaatgacaa atggaaccat caccattgag aatggaatta cctcttctgc
    26821 catcccgaat gtagcgccac cgaatgaaaa agcaaagaat aatagcggta aagctaaacg
    26881 ctccagtccc ttagttttac tgataaacgc atagatacct gcgtcgatcg ctccggtacg
    26941 cataataata ccaaaagcac ctccaacaac aaggatcaat gcagcgatcc ctacagcaga
    27001 tccatactta tctccagata ctagtccttc aaaaacaaaa tttaagaaac caaagccgcc
    27061 aaaatcatct gttccccata ttttggctgt tttatgcaat tttttagaag tatcatagat
    27121 attttcgccg tactgatcgt aaagcacatc atctgtcaag gagatttcat ctagtttttc
    27181 ctgcgtaaga tttttttctc cgtcagcaat gactttttcc aatccttttt ccggaacatc
    27241 cagtttttca cgttctgctg gatgatcttg cagttcttct aattgatcaa acacatacga
    27301 cttatccagc tcgtaagcat aacggaagga atcttgacgt aaaacagtac gagtacttgt
    27361 ttcgccattt gcatctttgt attcaatatc ttgcgtgctg aactttcctg ctggaacgac
    27421 aaaagtcagt atccatgcaa agatgactac accaaaaata atgacatacg tatggggtgt
    27481 cgataattta gtgaagtcgc gtttcggttt tcctgaagaa tttgttttct tcaaatttat
    27541 ttcttccttt cttttttcag taattttgat g
//
LOCUS       DANJMI010000037        25396 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000037 DANJMI010000000
VERSION     DANJMI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.037"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            45..599
                     /locus_tag="PWU81_001914"
     CDS             45..599
                     /locus_tag="PWU81_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1110609.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(772..1245)
                     /locus_tag="PWU81_001915"
     CDS             complement(772..1245)
                     /locus_tag="PWU81_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1110610.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKIKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1257..1793
                     /locus_tag="PWU81_001916"
     CDS             1257..1793
                     /locus_tag="PWU81_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1110611.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1833..2204
                     /locus_tag="PWU81_001917"
     CDS             1833..2204
                     /locus_tag="PWU81_001917"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL1110612.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2488..3759
                     /locus_tag="PWU81_001918"
     CDS             2488..3759
                     /locus_tag="PWU81_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL1110613.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4004..5029
                     /locus_tag="PWU81_001919"
     CDS             4004..5029
                     /locus_tag="PWU81_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1110614.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5323..6432
                     /locus_tag="PWU81_001920"
     CDS             5323..6432
                     /locus_tag="PWU81_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL1110615.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6515..7411
                     /locus_tag="PWU81_001921"
                     /pseudo
     CDS             6515..7411
                     /locus_tag="PWU81_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7427..8251
                     /locus_tag="PWU81_001922"
     CDS             7427..8251
                     /locus_tag="PWU81_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1110616.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8283..9593
                     /locus_tag="PWU81_001923"
     CDS             8283..9593
                     /locus_tag="PWU81_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HDL1110617.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9741..11000
                     /locus_tag="PWU81_001924"
     CDS             9741..11000
                     /locus_tag="PWU81_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL1110618.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11010..11882
                     /locus_tag="PWU81_001925"
     CDS             11010..11882
                     /locus_tag="PWU81_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1110619.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11894..12724
                     /locus_tag="PWU81_001926"
     CDS             11894..12724
                     /locus_tag="PWU81_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1110620.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12764..14947
                     /locus_tag="PWU81_001927"
     CDS             12764..14947
                     /locus_tag="PWU81_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL1110621.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15008..16228
                     /locus_tag="PWU81_001928"
     CDS             15008..16228
                     /locus_tag="PWU81_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HDL1110622.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16225..17685
                     /gene="gtfA"
                     /locus_tag="PWU81_001929"
     CDS             16225..17685
                     /gene="gtfA"
                     /locus_tag="PWU81_001929"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HDL1110623.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17762..18868
                     /locus_tag="PWU81_001930"
                     /pseudo
     CDS             17762..18868
                     /locus_tag="PWU81_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19190..19399
                     /locus_tag="PWU81_001931"
     CDS             19190..19399
                     /locus_tag="PWU81_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL1110624.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19392..19541
                     /locus_tag="PWU81_001932"
     CDS             19392..19541
                     /locus_tag="PWU81_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDL1110625.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19695..20360
                     /locus_tag="PWU81_001933"
     CDS             19695..20360
                     /locus_tag="PWU81_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1110626.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20644..21729)
                     /gene="xerS"
                     /locus_tag="PWU81_001934"
     CDS             complement(20644..21729)
                     /gene="xerS"
                     /locus_tag="PWU81_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDL1110627.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22063..22296
                     /locus_tag="PWU81_001935"
     CDS             22063..22296
                     /locus_tag="PWU81_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HDL1110628.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22293..22700
                     /locus_tag="PWU81_001936"
     CDS             22293..22700
                     /locus_tag="PWU81_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL1110629.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(23837..24355)
                     /locus_tag="PWU81_001937"
     CDS             complement(23837..24355)
                     /locus_tag="PWU81_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HDL1110630.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         8636 a         3888 c         4744 g         8128 t
ORIGIN      
        1 tacataatca ttaactcaga atatttttaa gaagggaaac aatagtgagc tataatgtac
       61 aaccgttaag gacaaatgaa gagatacaag attttcagtt ttggctaaga agaacaagta
      121 atccggaaag agatagtttt ttatttttgt ttggaatcaa taatgggtta cgtatgtcag
      181 atattatagg attaaaagtt ggagatatac gaggcaaaag taagcctatt attgttgaac
      241 ataaaacagg taagcgaaaa ccgattttta tagataattt gagagaagaa attttattat
      301 atactgaagg taaagaagaa aatgattggt tatttccgag tcggcaacaa ggtcgacata
      361 taacaaggga tcgtgtctat cagatatatg cagatatagc agaaaaatta ggacgagatg
      421 atataggaac acatacatta agaaaaactt ttgggtatca ctactacaag aaaactagag
      481 acattgctac cttaatgttt atttttaatc atagctcgca agcaattaca aaaaggtaca
      541 ttggtataac tgaagatgaa ataggagcat cacttcgtgg atttaaacta ggtgtataat
      601 ttaaaaattt ttataaacag taaagacaaa gattaaaagc tatttttatc tagaacgatt
      661 ttatttcata aagttcgcgg aataaaagct ggggttactg gtacttggta taggttcgtg
      721 ttatataaac taagtaatta aataacattt ttaggttgga aaaggatgaa attaaaaaaa
      781 gagaatcaat acggtctcaa aaacgcaaac gctgcggaat aagaaaaata ctgataactc
      841 aacgtttagc agactatcta atagggatag tctgcttttt tcgtttttga ccatactttt
      901 gatcttctgc aaacttcctt gcttttcctc tgtagaactc atgaaatctg caaaacgaat
      961 tgccgtttcc tctgtctgct ttttcgcgat atgggtgtat atatcatcca tagtggtttt
     1021 aatatctgta tgtccaagtc gaacatacac ttctttgaca gacgcaccag attcaaagag
     1081 cagactacaa tgggtatgac gaaaaccatg aagtgtgata tgttttaagt tattctcttt
     1141 aatgatgatt ttcaaattgt gatttacata gtctaaataa aggggcttat tttcttctga
     1201 tgaaaacaaa agttgttcac catcttttat aggtttgatt ctcattttta gatattatgc
     1261 ccgcacgtat tcatcacatt tctattgtga atcgctttat tcgaccgaca tttgactttt
     1321 atcataatat actcggttta aaattactaa tgaaaacaat taatcaagac gatcatacca
     1381 tgtatcacct gtttttttca gataatcacc accgtgtggg aactgaactg actttttttg
     1441 aagtacaaga agggaacaac cagttttttg gaaccaatac gattgaacga acgatactaa
     1501 aagttccttc cgaagcatca ttgcattttt gggaaatata ttttgaaaca caaggcgttt
     1561 gccattatgg aatcgaatca ttttctggac gacctatctt acgttttgaa gggccagatg
     1621 caacacgatt agctttagtt cccttacgcg aatttgaaga tattgatgat tatttcccgt
     1681 atgtcacaga ccagatccct acagagcatg ccattttagg aattgatgcc attcaactac
     1741 gcgtacaata ttccgatgcg actgaacgag agttattgtc ggtaggctgg tagcacaaag
     1801 aatacgtcag tttcttttcg ggtcatcaac gagtgacaat cttagaaaat caagacactc
     1861 agttctatca agaagttcag attattcaag atcgtgagaa tccagttgcc attgaaggga
     1921 ttggtagcgt ccatcatgtt gcgtttggcg tagaaaataa atctgattta caacgcatcg
     1981 acaaacaatt acaagagcgc aattttataa attcaggaat aaaagatcgt gaattttttg
     2041 tatcacttta ttatcgtgac ccaaatcaat tattgattga aattgctaca ggggaaggaa
     2101 acctagatgc aaaagcgtat gaaaatcaaa gtccacgctt tgaagaaatt cctttatttt
     2161 taccgcagta cttaaatttc attcgtgaac aagtggagca ataattagct aatcaaatcc
     2221 atgacgaaga gtaagaggtt gactcataag tcaaaaaata aagaaaaacg catcaaatcc
     2281 attctaaaga atgttgattt gatgcggttc ctatttttat aaactatcat tttcgactaa
     2341 taaaactact tatgagtcag cctctctttt tttcttgatt tttatacctc tttctaggta
     2401 aactaaacat ctgccaaaaa taccgtgtct cattgaaaca ttctcaacta tttcaaaata
     2461 aaggggagta ccaaatgaac gagtatattg aaattatcca cgcaagagaa acaacttaaa
     2521 aatgtcagtc tcaaaattcc caaaaataaa attagtattt ttaccagcgt atctggttca
     2581 ggaaaatcat cgattgtttt tgatactatt acccaagaag ctggtcgtca actgaatgac
     2641 acctatgata gttttacccg tttattttta cctaaataca gtcgtccaga ggttgatgag
     2701 attgataatc tttctacggc gattgtggtc aatcaaaaga agattggtgg cagtgcgcgc
     2761 tcaacgttag ggacgattac taacatcaat gcgttatttc gtgtgttgtt ttcatgtttt
     2821 gcaacaccta gtttaggata tgcaaatgct tattcattta atgatcctag tggaatgtgt
     2881 tctgtctgtc agggaatcgg taagacgatt gcatttttaa taggattgct ttatttttcg
     2941 gctattgata atgctgattt tgtattttct gatccattta ccgcagaagt tttgtctgga
     3001 attgaggaac aggcaaagaa tcatggttat tttgtattgg tgcataatgt taattcagca
     3061 gaagatgtta aaactattca gagaaattgg cgttttgagg ggttcattgt tgttggtgta
     3121 cgtgtaaaag aatttttgga attaaataac gtttttgatg gtcctgttgt ttatattgat
     3181 acgcatcttt caaaagagac attaagagaa cttgaacaac aaatgaatcg tttatttgta
     3241 aatacaaatg attaccaagc tagtacattg gcagtggaac atttgattca ttatggtcat
     3301 gagaaaatag cgtttctttc ttttgactac attgtaggtg aaccaagtgt tattcaagaa
     3361 cgacatcaag cttatatcga tacattaaag aaatatcatt taacctttaa atcgcattgg
     3421 atatataaca actctgaatt tgagcgaatt tatcaagaat tagctgaatt tactgcaatt
     3481 gttgttactg gagattattt agcggctaaa ttagtaaaat ttttaaaaaa taagaataaa
     3541 cgagtgatgg aacaattatc gattattgga tttgatgata ttacttttgc agaattaatg
     3601 gatcctgcgt tgacaacaat acgactaaat ccaggtaaca agggggcgat tgcttttcac
     3661 caactgattg gactggccaa acaagaagaa ataaaaaatc aagtactact attagaggga
     3721 gaattgattt ctcgagactc tgttcacaaa atcaaataaa aaagctctgt gaaataagta
     3781 tagttaacaa taaaatatcc gaacgataga gagaatgaac tgtgatctag cagttcctcg
     3841 tcgcaaattc actagaatta gcattattta ctctaaattg ttcggatatt ttttcttgtt
     3901 tttaattatg attacagtgc ctatttttaa cgaaatttgc ttattttcaa aatgtatgat
     3961 tatatgttag taaacacttt caaatatgaa aagaggagct attatggtag gaattaggga
     4021 tgttgcaaaa cgggcgggtg tgtccattgc aacagtatcg cgcgtgctaa atgaggatcc
     4081 gacactttca gtaaccgatg aaaccaaacg aaagattgca gaatcggtta atttttatca
     4141 ttatgtcaaa aaaagtcccg ttgcaaaaaa tttaacaaat attgcattaa taacgacagt
     4201 ttctgaagta gatgagttgg aagatcccta ttttcgaacg attcgcagag ggattcaagt
     4261 cgaagcagaa caaagcaaag tcaatttgaa gaaaattatt cgtttgtcag aaacaccgtt
     4321 ggagcaagac gatttgctaa aatgtcaagg tgtgttgatt attgggcagg tgctatcttc
     4381 tgtgattgaa gaagtatcag caattaattc gaatgtcgta gtgattgatg accctagtat
     4441 tgaagctgaa gtagatgcag tttatactga tttaacaaaa gcaacgaatc agcatttaga
     4501 tcgattgtat aaaaaaggac accgaaacat tgcgtttatc ggaggaggac gtgttcgttt
     4561 agatagccaa ggaaataaac acgtgtcaga tgatgattat cgaaaaatag cctatgagac
     4621 gtggatgaaa caaaaaggat tagaaaaata tagtcaaatt tatttagaag gctggaccac
     4681 acttgacgga atgcaggcag cagatttact tttgaaaaaa catgctaaag ataagttgcc
     4741 gactgctatc atggtaggaa atgatcctat tgctgtgggg gtttatcgtt cacttcaaaa
     4801 aaatggtcta catattccag aagatatttc gattgtgagt tttgataata ttgaagttgc
     4861 tgaattttta acacctgcat taaatacggt gaatgtgaat acagaagaaa ttggaaaact
     4921 agcagttcgc atggttaatg aacgaataaa agaagttaga aatgtttcga tcaaagtgat
     4981 tgtttctaat aatattattg ttagagaaag tgaaaaaaat atcgattaaa aaagtaaaca
     5041 tccaaaaaag ggtgtttact tttttgttta ctaatatttt tagtaaaaaa tctcgacaaa
     5101 acattaagta aacagttatg gtgttgttaa caaaaagaaa acgcattcaa aacaggagga
     5161 attaagatga aacatttcaa aaaaatagct ttaacaggga tagctgtttt aactgggaca
     5221 ttactcgcag catgtggtgg gggagatagt aattctaaag acgggcaagt aactttaagc
     5281 tatgcgagtt gagatactga ccaagctcca ggactaagaa aaatgttaga tgaatttgaa
     5341 aaacaaaatc ctgatatcaa agtagaaatg gagacaacgc cttgggatca atattgggtt
     5401 aaattagaag ctgcaacaac gggaggtaat atgccagatg tagtaaccat gcactctagc
     5461 gaatcctata aatatatgtc acagaatgct ttgatgggac ttgatgatgt tatcaaagaa
     5521 aatgatattg acatgggaaa ctttactgaa ggaattgctg atttctatac gcttgaagac
     5581 gaactatacg caatccctaa agatgcttct gtagtggggc tatggtacaa caaagaatta
     5641 tttgataatg caggtgtcga ttatccagat gaaacatgga catgggaaac actcagacaa
     5701 gctgcaattg atttaacaga tgaatcaaaa ggcatctatg gttttgcatc acatattgga
     5761 acagagactg gctactggcc atttatttat caaaatgggg gagaagtatt tgccgatgac
     5821 aatacaaaat caggtttgaa taccccagaa gcacaagaag ctttagcttt ttatcgagat
     5881 attgcacttg ttgatcatgc ttcaccaaca attacagaat tacaagatcc tgataagtta
     5941 agtcgttttc aatcaggtag attagcaatg atgattgaag gaaactggtt tgcaccggga
     6001 tttttgaaaa atgactatct agttgaaaat gttgcttctg ccgtgttacc taagggaaaa
     6061 gttaatgcaa caattacaaa tggacttggt tgggctgcca gtgcgagtac aaaataccca
     6121 gaagaagtga aaaaactaat ggcattttta gctagtgagg acgccaataa aatccaagcg
     6181 gaaacggggg catcaatccc agcactaaaa ggctttgaag acgagtgggc tgcttcttca
     6241 gatacaattg atttgagtgc ttttggaaaa atgttggagt atggagtccc tcgtccatac
     6301 aataaagatg gattacgagc tgaacaagca gaagttcaaa ttattaaccg tattctgtct
     6361 ggtgaagtaa ctgttgaaga tggaaccgct caaattgaaa aagaagtcaa tgcgatattg
     6421 agcggaaatt aaattagtta attatacaat atacaggcga aaggattcta tttcgtctgt
     6481 atattaatgg atgaatacca aaaaggggga tattatgaaa aatcgaattt ttggaaaata
     6541 tcgtaaagat caagtgatat ggggctatat ttttattttt ccaacggcat taggattaat
     6601 tttattaaat atatggccag caattcaaac agcaatttta agttttcaaa aaacggtagg
     6661 gttcggtgat actgaatggg tcggattaag aaattaccaa aaaatgtttg cagacggtga
     6721 agtttttcag tctttattaa acacattaat ttatgcagtg gtttcgatac ctagtattgt
     6781 tgtattatcc ttgttggtgg cagttttaat gaatagaaaa gttaaaggat tgtcgattta
     6841 tcggacaatt tattttctac cagtcgttgc tgcaccatcc gcagtcgcaa tgatttggcg
     6901 ctggatgttc aataatgatt atggactgat taataacatg ttggctaaaa taggtctcga
     6961 tggaccagca tggttaacgg atccatcgat tgcgatttat tctattatca ttgtaggagt
     7021 ttggagtgct attggttata acatggtgtt attactagcc ggcttataag aaattcctaa
     7081 agattattat gaagcagcgg aaattgatgg tgctagtcca atccagcagt ttttcaacat
     7141 tacgttacca ttagttacac caacattatt ttttgtagtt gtaacaacag ttatcaatgc
     7201 tttccaagta tttgatgtta tttttatgat gattactcct aatagtaccg ccatgtttaa
     7261 atcacaatca ttagcctatt tattctataa acattcattt attttaaatg acaagggata
     7321 tggttcggca atcgttatgt tccttttagt gattatatta attattactg caattcaaat
     7381 gaagttgcag aaaaagtggg taaactatta aggaggctat caaattatga aaaataaatt
     7441 tttttcaaat gcgccaatcc atatcatgct tattttaggt ggtattgcaa tggcattacc
     7501 ttttatttgg atgattttaa cttcagggaa aacgttggtt gaatctaccc aaattccacc
     7561 tacaattttc cctgagaaat ttcagctaag taactatcaa caagtacttg atttattacc
     7621 attcatggct ttttatatca atacattttt aatggttgtt ggacgagtga ttggctcggt
     7681 tttattttca gcaatggctg catatgcttg tgcgcgtcta agttttccag ggaaaaatat
     7741 ccttttcagc ttagtactta ttcaaatgat gattcccggt tccattttta ttactccaca
     7801 gtatttatta gcacaaaaat tgggtatttt aaatacaatt ccagcattaa tattaccagg
     7861 ggtagttagt gcgtttggta cgtttttatt aagacaattt ttcttaggtt tacctgatga
     7921 attggaggaa gcggcctatt tagaaggtgc caatatttgg acgatctttt tcaaaattat
     7981 gttgccgctt gctaaatcag gattggttgc acttggaata ttcacagcgt tgtttgcatt
     8041 taaagattta atgtggccat tgattgtcaa tacttcaatt gataagatgc ctttagctcc
     8101 gggattagca aacttacaag gacaatatgc cacgaacttc ccacaattaa tggcaggttc
     8161 gttattagca atttggccaa tagttgtgat attcatcgtt ttccaaagaa aattcattga
     8221 aggaattgct acaacaggta gtaaattata aaactattaa ttagtgattg gagagtaagt
     8281 acatgacaaa aattacattt attggtgcag gaagtactat ttttgcaaaa aacgtattag
     8341 gcgatgcgat gttaactcca agtctacaag atgcacatat tgcattgtac gatattgatg
     8401 aaaaacgact aaaagaatca aatttaatgc ttaaaaatat taataaaaat tcgaatgaaa
     8461 atcgtgctga tattgtttct tattatgatc gcaaagaagc gttaagagag gctgattttg
     8521 ttatcaatgc tattcaagtg ggtggttata aaccttcaac agtgattgat tttgatattc
     8581 ctaaaaaata tgggttgcga caaactattg gtgacaccat ggggattggt gggattttta
     8641 gaaccctacg gacactacca gtaatgttag actttgctaa agaaatggaa gaagtttgtc
     8701 caaatgcttg gttattgaac tatacaaatc cgatggcgat gttaaccatg ggtgttttaa
     8761 aagcgacgag tattaagact gttggtttat gtcatagcgt tcaagtatgc gtaccagaat
     8821 tgtttgagca tttaggaata gccgatcaat atgacttaaa tgactttcaa tggaaaattg
     8881 caggcattaa tcatatggca ttcttgttgg aaattacaaa aaatggggaa gatttctatc
     8941 caacaattcg tcagttagct gcagaaaaac caaatccaca tccagattct gtacgttttg
     9001 aattgttgaa acgttttggt tactatatta cagaatcaag tgaacacaat gcagaatacc
     9061 atccatactt tattaaatca aaatacccag aattgattga cgaattaggg attccgttgg
     9121 atgaatactt acgtcgttgt gaacaacaaa ttaatgattg ggaaagcatg cgagaggaca
     9181 ttgtaaataa tggggagtta acacatacga gaagccgtga atatgcttca tatatcatgg
     9241 attctattac gacaggaact ccgtcagtga ttgcaggaaa tgtcttaaat aaaggcttaa
     9301 tcacaaactt accgacagat gtttgtgtgg aagtaccttg tttagtcgat aaaaatggcg
     9361 tgcaaccaac ctatgtcggt gaattgccaa cacaattagc tgctttaaat cggacgaata
     9421 tcaatgttca agaactgaca gtagaagccg ctttaacact agataaatca aaaatttatc
     9481 aagcagcgta tttagatcca catctatcat ctgaattgtc tttaacagat atcaaaaatt
     9541 tagtggatga tttaatcgaa gcacatgggg actatttacc tgaatataat taaaaagaag
     9601 ctgaaagagt aaggaatttt caattcctta ctcttttctt agttaaattt agtaaaaata
     9661 ttgacaaaac acttagtaaa cagttacatt agtgttaaca aaaagaaaac gcattcaaat
     9721 tgtataagga ggagcataaa atgaagtggt ctaaaaaaat tattttagct tgtatagcac
     9781 taggagcaac gatctctttg actgcttgtg gaaaaaatca ggaagatgca agcggtaagg
     9841 tagaaataga gttttttaat caaaaaaaag aaatgactca aacgattcag gagattgcta
     9901 aagattttga agcaaaaaac cctgatattc atgtaaaagt tgtggatgta ccgaatgctg
     9961 gtgaagtaat taaaacacgt atgcttgcag gcgatgtacc agatgtcatt aatctttatc
    10021 ctcaaagtat agaattgaaa gaatgggcga aagctggtta tttagaggac ttaaccgaag
    10081 aaccgtattt agagaatatc aaaaatggat atgcacaacg ttttgctatt gaggatcgtg
    10141 tatatagcat accattaacc gctaatgtct atggttttta ctataacaaa actgcttttg
    10201 aggaaatggg gattcaagca ccagaaactt gggcagaatt tgaaaaaata gtagctgaga
    10261 ttaaagatca aaacaaagta ccgtttgcga ttgcaggagc agaaggctgg actttaaatg
    10321 gttatcacca actagctata gcgactgctg ctggtggtga agaggaagcg aacaatgttt
    10381 ggcgtttctc agaagttaat ggaattaatg ctgaaagcaa agaaatgcaa aaagatttta
    10441 atcgattaga tttgttaaga gaaccaaaag ctttgcaaaa caactggcaa ggtgccggtt
    10501 ataacgatac agtagttact tttacaaaag gtgatgcgtt gattatgccg aatggttcat
    10561 gggcaatgcc gatgattcat tcacaaaatc cagattttga agtaggaact tttccttttc
    10621 cagcagatga agcaggtcaa agtctaacga ttggcgcagg agacctagcc ttatcagttt
    10681 ctgcaacttc tcaacataaa gaagaagcca aacgttttgt agaatatatg acaacacctg
    10741 aagcgatgca gaaatattat gatgtagatg gtgcaccgtg tgctgttgag ggcgttatcg
    10801 agaatactca ggattcacca ttaagtggtt taactgaact tgcttttact gatcgtcatt
    10861 tagtttggtt agccaaagat tggaatagtg aaaatgattt ttatacctta acaacaaatt
    10921 atttacacaa tggcaatcaa aaagccatga ttaacgcatt aaatgctttc tttaatccaa
    10981 tgaaagctga cggaaggtag gaaaataaaa tgaaaataaa agaaatttta aatcgttact
    11041 gggcaatcat ttttgtagca attccaatta ttttgcagat gatttttttc tactttccaa
    11101 tggttcaagg tgctttttac agtttaacta attggactgg tctgacttat aattttgatt
    11161 ttgtgggttt gaacaattat aagattcttt tttctgatcc gaagtttatc aaatcgattg
    11221 gcttcacttt aattgtgacg ctttgcatga ttgctggaca aattggtttt ggtattttga
    11281 ttgcgcgggc gttgaacgca aatattaaag ggaaaacatt ctttagagca tggttctttt
    11341 ttccagcggt cttatcgggc gtggttgttt ctttagtatt taaacaaatt tttaactatg
    11401 gtttaccaaa aattggtagt atgttaggca ttgaattttt acaaaccagt cttttaggaa
    11461 caacatgggg agcgattttt gcagtaattt ttgttttact ttggcaagga attgccatgc
    11521 cagtcatcat tttcttagcg ggttttcaaa gtattccaca agaaattatt gaagctgcta
    11581 gaatggatgg ggcgaatgat aagcaaattt tttggaatat tgaaatcacc tacttattac
    11641 caagtatttc aatggtattt attatggcat taaaaggtgg cttaacagca tttgatcaaa
    11701 tttttgcttt aactggtggt ggaccaagcg atgccacaac atctttgggg ttacttgttt
    11761 ataattatgc ttttaaaaac aatcagtttg gttatgcgaa tgctattgct ttagtcttat
    11821 ttatcttaat tgggattgtt tcactggcac aattaaaatt atcgaaaaaa tttgaaatct
    11881 aggaggtttc ccaatgaaaa catcaaaaga aaaaaatatt tctaaatact tgcttttatt
    11941 attagggggc ttcatgattt tagttccctt agtttcaacg ctattcggtt catttaaaac
    12001 aacacccgat attatgaata atttctttcg ttttcctaat ccagcgactt tggaaaattt
    12061 ccgaagattg ttagcggatg gaattggtca ttacttttgg aattcggcaa tcatcacaat
    12121 cattgcagta gttgctgtga cagcaattat tcctatggct gcttattcac tagcaagaaa
    12181 aatgactcgt gggaaagcat acggattgat gtatttattt ttaattttag gaatttttgt
    12241 tcctttccaa gtaattatga ttccgattac cgttatgatg agtaaaattg gtttagcaaa
    12301 tatgtgggga ttaatcttac tctatctcac ttatgccgta ccacagacac tatttttata
    12361 tgttggtttt attaaactaa atgtgcctat cagtctggat gaggctgctg aaattgatgg
    12421 ggctagtcac tttacaactt atttcaaaat tgttttccca atgttaaaac caatgcatgc
    12481 gacgactatt attcttaatg ctctatggtt ttggaatgat ttcatgctac cattattaat
    12541 tttaaatcgt gattctaata tgtggacact accattgttt caatataatt atacgggaca
    12601 atattttaac gactatggtc caagttttgc ttcctataca gtaggaattg tcacgattac
    12661 actagtttat cttgctttcc aaagaagtat tatagctgga atgagcaatg gagcagtgaa
    12721 ataaccaata aattattctc attataaagg agttcataaa cttatgatta tatttgacaa
    12781 tcacacacaa acatttcatt tacaaaataa aaaaattagc tatctattag gaatcgaaga
    12841 aaataattat ttaacgcatc tttattttgg aaaaaagatt acgcactatt ctggtgggta
    12901 tcgctacccc cgaacagatc gttccttctc acccaacaat gcaaatgcgc aagatcgttt
    12961 attatcttta gacacattac cattagaatt tccaggatat ggacatggtg attttagaga
    13021 accggcgcat aatattaaat taggaaacgg tagtcgtgta aatgattttc gttatgatag
    13081 ctacgaaatt caaaaaggaa agcccagctt agcgggattg ccagccttat atacggaaga
    13141 tgatgatgaa gctgaaacgc tgattattca attgaaagac gcgattagtg gattgacttt
    13201 acagttgttc tacacaattt atcaaaatca cagtgtaatt attcgttcta cgcaattagt
    13261 aaataatagt gatgaaacag tggagattaa ccgcttagct agtcaatcaa ttgattttcc
    13321 aaatcgtcca ctcgagttaa ttcatttaaa tggtacctgg gcaagagaac gtcaaatgac
    13381 tagagaaaaa attcatacag ggataaaagt attagatagt aaacgcggat caagcagcca
    13441 tcaacaaaat ccgtttgttg ctttagtaga tgccaaaaca gatgaatttc aaggagaggc
    13501 atatggattt aatttagttt atagcggtaa tcacgaaacg gtgattcaaa aagatccatt
    13561 tgaccaaaca cgggtcatta tgggaattaa ttcatttaat tttggttggc aactaaaaac
    13621 tggcgactca tttcaaacgc cagaagtcgt gatggtttat agtaatgaag gtctgaatac
    13681 gttatcacag acgtatcatg aattgtataa tcaccgttta attcgtggaa actatcgttt
    13741 aaaagaacgt cccacgttaa tcaacaactg ggaagcaacg tattttgatt ttaacgaaga
    13801 aaaaatcatg aatattgtcg atgatgcgaa gaacttgggc attgaaatgt ttgttttaga
    13861 tgatggttgg tttggcggtc gtgaaaatga cttcacttca ttaggtgatt gggttgaaac
    13921 agaaggcaaa cttgcttgtg gtttggaaaa tttagccaag caagtgcatg ataacgggat
    13981 gaaatttggg atttggtttg aaccggaaat gatttctgaa gatagtgaac tatttcgagc
    14041 acatccagat tgggcattgg gtattccagg tcgtgaccgc tcaattagtc gaaatcaata
    14101 tgttcttgat ttttcaagac aagatgttcg tgataatatc tatcaacaaa tgacggcgat
    14161 ttttgatcgt gtaccgattg attatgtgaa gtgggatatg aatcgaaata tgacagaagt
    14221 ctattctgtc ttgttggact caacgatgca aggtgaagtg gctcatcgtt atatgttagg
    14281 gttatatgaa tttttagaaa agttaacaac gaattatcca actattttat ttgaaagttg
    14341 ttcgggtgga ggcggtcgtt ttgatgcagg tttcttgtat tacatgccac aaacttggac
    14401 gagcgacaat accgatgccg tggctcgtct gaaaattcaa tatggaacca gcttagtgta
    14461 tccaatttca agtatgggtg cgcatgtttc agcagtccca aatcatcaaa caggaagaga
    14521 aacgagttta actattcgtg gcaatgctgc gatggctggt gtttttggtt atgagctaga
    14581 tctaacgcaa ttaacttctg aagaaaaagc agaaattaaa gaacaagttg ccttttataa
    14641 aaagcatcgc caacttttgc aatatggaac tttttatcgc ttggaaagtc cgtttgaagg
    14701 caatgatact gcatggatgt ttgtctcaaa agaccaaaca gaagccatgg tcttttattt
    14761 ccgagtatta gcagaagctg cgcatccatt agtcacattg aaattagcag gattagcaga
    14821 agaagcaact tatcaaatgg aagatgtaac atttgctggc gatgaattga tgaatctcgg
    14881 tttttatatt gactcagaac tacatggtga ttatgcgaca caacgctttt atttgaaaca
    14941 gatatagtag cttgaattta caatataagt gcaacaccaa aagagactca ttccaaacga
    15001 agaaaatatg ttttatttac acgcatttac taaaaagcag cctgatttga attgggaaaa
    15061 tgaagaagta cgtgaagaaa tctatgcaat gattaactat tggttagata aaggactagg
    15121 cggatttaga atcgatgcga tattaaacat caaaaaaaaa ctagagtatg gcatctttga
    15181 atctgatgga gaagatgggt tagccttcat tgggccctgg attcttaatc agtcgggcat
    15241 tgaagtgtgg ttaaaagaaa tgagagagcg gacatttaaa ccacataata gtatgacggt
    15301 agcagaagcc gatgttccta atgaacgttt agatgaatat attggtgaag atggctatta
    15361 ttcgatggtt tttgatttta gttatacgga tattgatgta cctgaaactg gtgaatggtt
    15421 taaagatagc caatggacat ggacggatat gcgtaccaat atttttacga atcaattagt
    15481 tacgcaagat aaaggttggg gcgccttata tttagaaaac cacgatcaac cacgttcaat
    15541 taataaatat attccagaag aatggatcaa tgattatagt aaaaaaatgt tggcgacgct
    15601 ttttatgtta ctaagaggta ccccttttat ttatcaagga caagaactag gaatgaccaa
    15661 catagaaatg gcatcattag aagattttga tgatgtagct acgcattctc agtataaacg
    15721 ggcattagaa tatggattgg caccagagca agcattgaaa gctgtcaata aacgaagtcg
    15781 ggataattct cgcacaccta tgcaatggaa tacgcaaaaa aatgccagat tctcaacttc
    15841 tgataatgta tggttaaaag taaatcctaa ttatccagaa ctcaatgcag aagctcagtt
    15901 aaccaatcaa cagtcggtat tcaattatta tcatcaatta attaaattaa gaaaaaatag
    15961 tgtatacaaa gacgtgctaa tctacggaca atttctacca gtgaaagata aaaatgaaca
    16021 tattttgctt tatgaacgcc aattagaaga taaaatcgta ttagttatct taaacgttac
    16081 agcaacagaa caagaatatt atgtagatga acagtaccaa caagtattaa tcaataatta
    16141 tgaagatgtt ttattagatc aaagtcgaaa attgcgttta cgaccgtttg aaagtattgt
    16201 attagctaat tatggaggac aattatgaaa attaaaaacg aagcaatgct catcacctat
    16261 gcagatagtt taggaaaaaa catgggcgaa ttaaaacaag tcttagacaa acatttacaa
    16321 ggtgttattg gtggtgtaca tctgttgcct ttttttccat cgacaggtga tcgaggcttt
    16381 gcgccgagtg attatacgac agttgatcca gctcttggaa catgggaaga tgtggaagca
    16441 ttaggtgaag attattattt gatgtttgat tttatgatta atcacatttc tcgtgaatca
    16501 aaatttttcc aagatttcaa aaaaaaccat gagcagtcac catacaaaga aatgtttatc
    16561 cgtattcatg aatttttccc agaaaatcga ccaacacaag aagatattga tttaatctac
    16621 aagcgtaaag acaaagcgcc gtttcaaacg gtagaatttg cggatggtac gacagaagag
    16681 gtttggaata cctttgggga agaacaaatt gatttagatg tgaccaaaga agttgtgaaa
    16741 gaatttattc gagaaaccat caaagatatg gcgaaccatg gctgttcttt aattcgatta
    16801 gatgcgtttg cttatgcgat taaaaaatta gataccaatg atttcttcgt ggaaccagat
    16861 atttgggaac ttttagacga agtgcgtcaa gaagcagcga aatatgacgt cgaattatta
    16921 ccagaaatcc atgagcatta ttccattcaa atgaaaattg ccaatcatga ttattatatc
    16981 tatgacttcg ccttgccaat ggtcactctg tattcacttt atagtggaaa atcagaacgt
    17041 ttagccaatt ggttgaaaat gagtccgatg aagcagttta caacattgga tacgcatgat
    17101 ggaattggtg ttgtggacgc tcgtgattta ttaacagatg aagaattgga ttacacctct
    17161 gaggaattgt ataaagtcgg ggcgaatgtg aagaaggtct attcaagtgc caattacaat
    17221 aatttagata tttatcaaat taacagcacc tattatagtg cattaggcga taatgaccgt
    17281 agctatctgt tagcacgtgc gattcaagta tttgcgccag ggattccaca aatttattac
    17341 gttgggttat tagcaggaaa aaatgatatt gaactgttgg aagcaacgaa agaagggcgt
    17401 aatattaatc gtcattacta tgatttagcc gaaattgaag aagaagtgca acggccagtg
    17461 attcaacaat tatttgcact tttaaaattt agaaatgaat ctgcggcgtt tgatttggat
    17521 ggaacgattg aagtagcaac gccatcagaa tcagaaatag tgattacacg taaaaataaa
    17581 aacggtagtg atagtgcaac attaacggct aacttggata cgaaagaatt tatcattgag
    17641 tctaaagata taaaaatttt cactagtgtt gcattaaaat tttaacctat tccatagaac
    17701 aaaaaatcct ttatactagc acttggatga cttatccaag accaataaaa aggactcata
    17761 catggataag tataaaacaa atacagcatt taataaatgg ttttctgcca taaaattagg
    17821 aaaactttct gtcaactccc aaaaatgtat ttttgattac aattcgtatc gtaaaaaact
    17881 gtcttttgaa gctgtgctca agctctttct ctacgcagtt aatgaagaaa aagaaagttt
    17941 gcgtgatttg agtacagctc ttatcgaccg atcgctccaa ctagaaaccg atgtgtcatc
    18001 gattagtcat acccaattgt ctcgagcatt taatgcactt gaacctaaag tgttagaaga
    18061 aatttttcaa caactgttag aaaaagttca acatcaaatc aaaccaacca agcgaaatca
    18121 tctgtatctg attgattcca gtacgttttc gcttagttta aaacgtcata agtgggcgac
    18181 tttccgtcaa acaaaatcag gtgtcaagtt acacttaaac tattgttata tggacgatac
    18241 tacgatgtat ccaactgatt ttaccttaac aaatgcccat gaacatgatc tcaatcaact
    18301 accagtattg gtgaatcaac cagaggcaac ttacgtgttt gatcgtgggt attaggactt
    18361 tgaaaagatg gatcagatgc attgggatgg ctatttcttc gtcactcgga ttaaaaaaaa
    18421 caccaaagtt cacgtgatag atactctgga aacatcgcca gaaacagaaa ttctacgaga
    18481 cgagctggtt cgtttaggat ctaaaaccta tctaaccgcc aatttccgac tagtcactgt
    18541 tcaagataag aacggacgtg tgtttcaatt tatcaccaat cgaatggacg tctcttcaaa
    18601 agaaatctct gatatgtatc acgctcgttg gcaaatcgaa ttgtttttca agcacatcaa
    18661 acaacacatg accattaaaa ccttcttttc tcaaagtgaa aaaggcgtgc agaatcaatt
    18721 gattttaacg atgatttccg ctctcctcac gtttctcatc aaattggaga cgaaaacgga
    18781 aaaatcagtg ttccaaatca aacgattctt tcgttattta ctgtttcagc cctttgaatg
    18841 ttgtctagaa aaattaattc ccacatgaga aaaaagaaat ttggctcgtt acttttggtt
    18901 ttcagttgaa taacagataa aaaattttct ggaaaaagtc atcctctgcg tcagcacagt
    18961 tttttttgac ttattttcag aggaaatagt tttcagaata ttctaaaatg ttttatgcaa
    19021 cactagtgat attttttaga attagatttc taatgtgcat tttttgagta ttaaggagca
    19081 agtatacaaa gatttataaa gctgattaaa tttgaataaa aatatgcagg aacgctacta
    19141 tttttagtag tgttcttttt tatatttgaa taaaaaatgt ggtggttaaa tggtggttat
    19201 aacaaaaaat gaattaatga aattaggtta tgggaagtat cagtctgaga gaatcatacg
    19261 acaggcaaaa gcaattatgg cttcaaaaag ctatacctat tacgaaaata gaagattagg
    19321 actagtacct gttgaatctg tagaagaagt tttaggtatg aagatagagg tagaggagga
    19381 ttttcagaat catggttaat tttaaaaaag ataaaaatgg gaaatatcat tttactgcta
    19441 gtttgggatt cgacaaacga acagggaaaa gagtacaaaa aaacgtagag gggttaaaac
    19501 agttaaagaa gaaaaaaagc gtataccgaa cttgtttcta attatggaaa agaagcagaa
    19561 acttctcaaa gtactatgtt gtttgaggag tttttttata aattattcct accttattat
    19621 caagggaaag tagaagaaag aacatataaa aatcggttat ctacaatcaa aaaaactttt
    19681 cttatttctt taaaatggag ttaagagaaa tacgtccaat tgacattcaa aagtggcaaa
    19741 ataatttatt gagcaaatat aaaaattctt atgtaagaaa catgtatggg atttttcaga
    19801 tgtcattaga tcgagcagtt gttttgggac tacttgaaaa aaatcctgca aaagttattg
    19861 gaaatgtgaa gaaaaggaaa actacagttg atttttggac aaaagaagaa tttacaaaag
    19921 ttatttcaac tttttatatt gatgatttgt accaacattt tggttttatt tgtatgtggt
    19981 tactttttat gaccgttatg cgtttgggag aagccactgc attaacttgg aaagatattg
    20041 attttgaaaa taaaaacttg cgtgtgaata aaacactcta ttataagaga gccaaaacct
    20101 atgagtttgt agaacccgaa acggcttcta gtattcgtac aatcgaacta gacgacaata
    20161 caattacctt tttaaaagct tggaaggagc gtcaagtttc aataggaggc atgtcgtttg
    20221 tactttctta taatggaaca cctactcaga aatatacaat ccgtcatatt atgagaagac
    20281 atgcaacgat cgttggtgtt catataatcc gcatacacgg attaaggcat agtcatgctt
    20341 ctttgcttat atcacaataa aaagatgcac ttcgaattaa agaaaggtta aaacatgctg
    20401 atgtacaaac gactttagga acttatggtc atttgtatct aaattcattg agagagataa
    20461 caaatgatcc cacagattat atccaaacta atttttctaa aatcaaaccg acacctattt
    20521 acgataataa aaaataaatg ataaacaaaa gtaggatcac accaaaagaa acacactaga
    20581 aaaactataa tacaggcttt tctagcgtgt tgtttcttat ttccgcctat ttatacatta
    20641 tactcataaa gcatttaatg catcgatcgt agccgcattg tctatatggg tatataaagt
    20701 tgttgtattg gtacttgaat gtcccaattg ttgggcagtt aataccaatg aatttgtctg
    20761 ttggtagagt ctagtagcta atgtatgacg cagtttgtga gggctgactt gaattctaaa
    20821 gccttttgaa tattttgcta ccatccgttc gattgcaata ccggatatac gcttggcttc
    20881 tccttggtaa attgctagaa ataaagcttt ttcgttttct gttactttat atcgttcttt
    20941 ccgaatagct agataattag ctaaatattc cattgcgaat gctgctatat tgacagcatc
    21001 tcttttattt ccttttctag ttactaccac attattctta tctaaagata ggtcatctaa
    21061 attaatattt ataacctcgg agagtctcaa tccagatcct aaaatcagtg cattgatcgc
    21121 caggtctctt tctacatctc gttgataata gatcaaagct cgtttactca aagtattttt
    21181 atattcattg gcaatataat ctagataatt ttgtgtatcc ttatctagaa ataatttttt
    21241 ctcaatggca ttggcacgtg cagctaacgt agttttatct gtgatggttg ctacttgatc
    21301 cataggatta ttgtctaagt atggttttcc tagaggatta ttggaacttt tcgaaagata
    21361 tttaaataga acctttaatg ccgtgatatt tctattgata gtagaagtag atagacctaa
    21421 gttattatta tctttgagct tagtttttgt tgtctctttt tttacatttt cttttgtgcg
    21481 acgtttcaaa tgagatttgt acaagtttag gtcataggca gttaactgtc ttatcgaggt
    21541 gatagaagta tcaggaacga tagactcact cagaagccat tcataaaaga gcttataatt
    21601 ttttagatat tctaaagtgg ttcgaggtga ttgatcttta tcctcacgat cttgaacaaa
    21661 atcttttata taatcgggca tatgaagtat gattgtatct attttttgat atatttctgt
    21721 atttttcatg tcattcatcc tttcaataag taggggagaa gagtatctct ataattggac
    21781 tataccaatt atagaatgtt ttgtcaattg gtatgctcta aaatttatta atttgtttca
    21841 aaaaaagcat ataaacaggt atacgtttaa taatttaaaa taaatggtat acataaatag
    21901 ggaaggaaat agaagggagc aatctaaatt ttttgaattt tcttaattaa ttatatagaa
    21961 agaacaatct ttctatagag ttagacatga gagggactat gcttgattta aaatcaacaa
    22021 gcgtatatac tgtatataca aagaaaaagg agggagaaga ctatggcaat taaagaaaga
    22081 aaatttaggg aagtggggaa ctcagtagta ttaactattt caaaggagtt tcttaaagaa
    22141 aatgggttaa atcccggaga tacggttttt ttagatgaag aaaaacttcg agcggcaatt
    22201 acaaaaaaac aagccttatc tgatgaaatt gatttagcaa tatctcaagc attaactgaa
    22261 tatgatacta cttttagaga gcttgtcgat aaatgagtat tcattaccta gaagccaaag
    22321 atattataaa aatgaatgta atccaaataa aaaaatactc tcctaatgaa ccccttggtg
    22381 taaaggatgc gaatgcttta gaaatgtgtg tagggcaact tcgagcctca gcttttggtg
    22441 aagaggttta tccttcgatt tatgaaaaag cagctatcct acttatacaa ttgataaaga
    22501 aacatccttt tcacaatgca aataaacgta ctgctttctt agctactttt gtctttttga
    22561 aaatcaatgg acagctttta gctattgatc aaaaagatgc tgtaaattta gcggtatata
    22621 ttgctacgta tgataaggat ttcgatcaac taaaatatga agtgatcaac actataaaga
    22681 cacatacgca gcccatttaa ggagttactg tatgtgtctt tatagggtta ttagctgcta
    22741 tagcgaaata attgaagtaa attacaaata gctataattc aaatccaact atatagaaaa
    22801 cgtaaatttt ctatatagtt ggattaagat aacgactatt tattttatca tatttttact
    22861 ataccctata cttttacttg gtatatttat gaataatcgc aaatttaccc ctatactact
    22921 agtttgtaag ttagattaat aaaaatacta aagttatttg ttcttttatt aaatacacct
    22981 acaacttata aattgatggt atttagataa cacgaaagaa taaaaataac agctgatagt
    23041 aaagtttgtt gacgattgct actcaatcga aagtgagtta gtgcgtatga acttgcttct
    23101 gaaaacttat gaaatgaaag accttttaca tcttgcagaa atcgttactg ggattaaact
    23161 aagcaataaa aactaatgtt cccctcattc tttgaacttc tactatttga tttatttatt
    23221 ctgataaatg acgatttcac cttcaggatc ttcaaatccc gtatagaatt tatttaagat
    23281 ctcttcagta tcagtaatag gataggcgat aggccacata ccctttgatg gttagaataa
    23341 actgctcaaa atctgtatcg tatgttggct ttagcatatt aacaaaggct atctccaaat
    23401 tataaaccat aagcgttgaa tcgttcttaa caatttcaat tcttcctaac ccaaagtcag
    23461 aagatataat taccggactt gccttaccct tcatgtgggt tgttgagatt ccatatttaa
    23521 agggaattgt cggtactatc ggcggtctga aaaacatccc aaaaataaaa aaatctttcc
    23581 cctgctagtc tcctaagtcc ctttgtagcg gatatggcac cataatctca ttttatcgaa
    23641 gtgagtcatg gtttatctgt tggccaacca aaattgatac aatctaacga tgcaccctct
    23701 acggcattct aggggagtgc acttgcattc acccaataaa aaccggcagt tagcaaagaa
    23761 cttatgtagt cgtatgttct tactgttaac atacaagagg tctcctttcc acaattgctt
    23821 agcagtaaac gggaatttac aaagctaatc tcttccattt tcttatcttt gttctaaaag
    23881 tcccaaatgg agcaactgta tttacatgaa taaatttata tacttcccat acagccgttt
    23941 tggttgcctc gtctgcccat tttcttttgt gtgcagtgaa tagatcttca tcactcattg
    24001 aatcaatcat catgttaata tcatcaatat tacgattgag aatttcctct aattctttca
    24061 atggtagatg tgcataggta tttgtaaacc acttatataa ctctcctaac tgattccatt
    24121 taaattgatc agaaggcgtt ttaacagata atccatttct ttcatcattt tcccatttta
    24181 gaattaaatt cgtccaacca acttgatatg ctaaattttc agcaggtgtt ctatccactt
    24241 cctcaactct tttatccttc aattcttcag gaatagtttc aaattctaaa atatatttag
    24301 tataagtcct atttatttct ttttttaatt cttctttatt tttatatgtt ctcattatat
    24361 tacctccata aattcctaat tgtctccttt aattcatcta taatttcatc cacgatattt
    24421 taatccgttc tacaatgcat atctgatatg cattgactgt ctaactcata tgtgaatgat
    24481 ttataaaata tcccaacgtc tcatctaaag aaatgttgat cgtcagcaag gagctaaaaa
    24541 atacgaatat atttcttttg tagtaaaatc ggactggttt gcatatcatt ttcttcgtaa
    24601 atatcaaaat catgttaact actatcaatt actaataaaa actaccactt taagcgttaa
    24661 gaactgttaa gtttagttaa gtgaggatag ttcacaaaat ccattgttaa acttcacgaa
    24721 agtggtaaaa ctcaaaatgc tttatatagg attacagcat cgctctttct tcgattgcgc
    24781 gttgggtaaa aaatattatg aagtaaaaat tgacgatgac acgttcctta cggctcatca
    24841 gatgaaacag ctacaaaaac gaaatgatca actcgaagaa aatttaattc taaaaaagcg
    24901 attgccatat tcacgcctcg ctcgacgaac tattaaaggt caagatttac ctgaagatca
    24961 tcctttttag gaaattgaag ctttttatgt tgcacaatgc atttataata ctacaatgat
    25021 gttttcacca gatattcttc tagggtgcgg tgtaatgaaa cagatacact tgcaaaagaa
    25081 aattcaccgc gaatttacta aaattatgaa taattatgtt aatcttccga aatttttttc
    25141 caaaattaag ggatcaagct ggtgttatag attgtttaag gttggcaaaa aaataacttc
    25201 tttttaaaca attatagaaa accgatactc tgttaagttg gttgacacgg tgtcgatttt
    25261 tatgactttc ttattctgaa acttcactaa cggacagtac ctaaaaagtc tatagtatgg
    25321 acgtttacga gtactatttt atcaaaaatg ctgctcattt caacttatat gttttagtga
    25381 actaccccca cttagc
//
LOCUS       DANJMI010000038        24260 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000038 DANJMI010000000
VERSION     DANJMI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.038"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="PWU81_001938"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="PWU81_001938"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL1110631.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="PWU81_001939"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="PWU81_001939"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL1110632.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="PWU81_001940"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="PWU81_001940"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL1110633.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="PWU81_001941"
     CDS             complement(3373..4680)
                     /locus_tag="PWU81_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL1110634.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="PWU81_001942"
     CDS             complement(4683..5264)
                     /locus_tag="PWU81_001942"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL1110635.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="PWU81_001943"
     CDS             complement(5836..7128)
                     /locus_tag="PWU81_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL1110636.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="PWU81_001944"
     CDS             complement(7125..7748)
                     /locus_tag="PWU81_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110637.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="PWU81_001945"
     CDS             complement(7751..8587)
                     /locus_tag="PWU81_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1110638.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="PWU81_001946"
     CDS             complement(8604..9491)
                     /locus_tag="PWU81_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1110639.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="PWU81_001947"
     CDS             complement(9507..10790)
                     /locus_tag="PWU81_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1110640.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="PWU81_001948"
     CDS             complement(11473..12156)
                     /locus_tag="PWU81_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL1110641.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="PWU81_001949"
     CDS             complement(12174..12791)
                     /locus_tag="PWU81_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL1110642.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="PWU81_001950"
     CDS             complement(12801..13625)
                     /locus_tag="PWU81_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL1110643.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="PWU81_001951"
     CDS             complement(13618..14178)
                     /locus_tag="PWU81_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110644.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="PWU81_001952"
     CDS             14352..15308
                     /locus_tag="PWU81_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL1110645.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="PWU81_001953"
     CDS             complement(15613..16569)
                     /locus_tag="PWU81_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDL1110646.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="PWU81_001954"
     CDS             16673..17275
                     /locus_tag="PWU81_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL1110647.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="PWU81_001955"
     CDS             complement(17363..18862)
                     /locus_tag="PWU81_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDL1110648.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="PWU81_001956"
     CDS             complement(18878..19402)
                     /locus_tag="PWU81_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL1110649.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="PWU81_001957"
     CDS             19700..20449
                     /locus_tag="PWU81_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL1110650.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="PWU81_001958"
     CDS             20440..21225
                     /locus_tag="PWU81_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HDL1110651.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="PWU81_001959"
     CDS             21194..21910
                     /locus_tag="PWU81_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL1110652.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22012..23046)
                     /locus_tag="PWU81_001960"
     CDS             complement(22012..23046)
                     /locus_tag="PWU81_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDL1110653.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(23050..24108)
                     /locus_tag="PWU81_001961"
     CDS             complement(23050..24108)
                     /locus_tag="PWU81_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL1110654.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7188 a         5222 c         4225 g         7625 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctccaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaatagtcc aagtatacag aagtaacaac
    21961 tgtttacttg gactattttt aaacatttta ttgacgaata tccatgtttt cttatcttaa
    22021 ttgacgggct ttaggaagag atacctcatt tttcgttttt ccagtttcta ttatttcacg
    22081 aaagttctcg aaacttgtcg aaatcatatt tgataatgcc tcatctgtat ttgaacctac
    22141 atgcggggtt acaagaactc tagggtacat ttctactaat tgctggatag cagggtctgg
    22201 aatcgtctcc catggcttga aggatcggaa aaaaacctct gtttcatttg caaaaacatc
    22261 ggttgcaaaa ccagctaaat gattgctttt caaagctctt aaaatagctt cattatcttg
    22321 aagctctcca cgggctgtat taatcaagat gctacctttt ttcatttgag ctaaaaatcc
    22381 ttcattgatc atttgatcgt tttcacctgg gatatgaggt acatgaatac tgacaatatc
    22441 gcttttggct aataactctt caagcgagtg gaacgtcaat acttttttag cagcttctgt
    22501 ctgatgaata tcgtagccga tcactttggc tcctagtccc ttaaacaaaa cagcttccgt
    22561 caaccctatc ctgcctgttc ctatgatccc tactgtgcaa tttctgattt cacgactaaa
    22621 catttgctca tccactcgaa aatctttgaa agatgttcgg atcgtcgtat acgcagtatt
    22681 tctcaaaagc atcatggcaa aagtcaaaga taactctgct atcgcatttg gcgaatacga
    22741 tggtacacgg gctactgtca taccaaaatc agctgccgct tgtagatcga tatgattgac
    22801 tcccacagtc cgtgtaaaaa cataacgaat cccatattcc tgcatttttg caagattggt
    22861 ctcatctgct atacaatttc ccctcaacaa tacagcatca tggtcctttg ctgacacgat
    22921 attgtcatgt gtcaacagtt cttcataaag actgagatca taatggtaaa tattcaattt
    22981 ttcaaaaaaa tctatctcgt tcggacgaac accgtaacat gcgattctca tactttcttc
    23041 ctccatttct tatgaaaata aacctatgcc atgaatcatt tgtaatacaa cgatccatac
    23101 aggtgtcagt accacagcaa aaatagttga cagtaaggat gcattggagg ctaaaagtgc
    23161 ctccctatca aaactgatag cataggctgc tgccactgtt gcagctggtg tagccatcat
    23221 gataacgatt gttgccagtg cttcaaaatt gactggtaga atattcgttg ccgtcaaaac
    23281 tgctagaatc acgatattca atgcaggtac aaataccact ttgactagac tgtaatacca
    23341 tgaagttttt tcagatgcag cttttttgaa gcttacttca cctaatgttg cgccgattgc
    23401 tagccacgcc aatggtgaac ttaaaccagc taaataatcc atcggctgaa atagccacgg
    23461 tgcagtctgg tcaattcgca aaaacgctac ttggcttatc tctccttctg cacctgtaac
    23521 tgacacttgt ggcagatacc cttggaacag ccaaatgaaa aggccggcaa aagtagcgat
    23581 aatgattgga ttgacgaaca tcgctttgat atttttagct tccatcttca agccactcat
    23641 ttttatgtat ccataagaat acaaaaagat tcggtagcca atattgaaaa ttgaggaata
    23701 catcacgccg acaggaccat aaatcgcact gacgataggc gttccgaaaa aggtcgtaga
    23761 accaaagatc gtcaggacac gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa
    23821 aggttttgaa actagaataa gaataatata aataactaat ccccaaatca agacattgat
    23881 tccttgcatc agtaattctg tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg
    23941 caaagccact gttaagacga ctttcgatag aatttttcca acagattcag aaaaaatccc
    24001 cttttttcta caaacaaacc ctaataatat aataaaaact gttgaagtga ttgcactgac
    24061 aatcgacata tctgtcaaag tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct
    24121 aattatgttc atttattcac aataaaaaaa gcttttattt tgctaatacg tattaacatt
    24181 tttaatttta tctttacatt gttacttatt gaacaatagg aaattaaaga aacttttgaa
    24241 acttatgtaa agtgattttt
//
LOCUS       DANJMI010000039        24249 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000039 DANJMI010000000
VERSION     DANJMI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.039"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            289..606
                     /locus_tag="PWU81_001962"
     CDS             289..606
                     /locus_tag="PWU81_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL1110655.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(697..1062)
                     /locus_tag="PWU81_001963"
     CDS             complement(697..1062)
                     /locus_tag="PWU81_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110656.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1270..1752
                     /locus_tag="PWU81_001964"
     CDS             1270..1752
                     /locus_tag="PWU81_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110657.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1949..2839
                     /locus_tag="PWU81_001965"
     CDS             1949..2839
                     /locus_tag="PWU81_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HDL1110658.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3165..5639
                     /locus_tag="PWU81_001966"
     CDS             3165..5639
                     /locus_tag="PWU81_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL1110659.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5742..7478)
                     /locus_tag="PWU81_001967"
     CDS             complement(5742..7478)
                     /locus_tag="PWU81_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDL1110660.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7475..8179)
                     /locus_tag="PWU81_001968"
     CDS             complement(7475..8179)
                     /locus_tag="PWU81_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1110661.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8310..8813
                     /locus_tag="PWU81_001969"
     CDS             8310..8813
                     /locus_tag="PWU81_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDL1110662.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8773..9111
                     /locus_tag="PWU81_001970"
     CDS             8773..9111
                     /locus_tag="PWU81_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDL1110663.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9132..10550
                     /gene="ffh"
                     /locus_tag="PWU81_001971"
     CDS             9132..10550
                     /gene="ffh"
                     /locus_tag="PWU81_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL1110664.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10662..11240
                     /locus_tag="PWU81_001972"
     CDS             10662..11240
                     /locus_tag="PWU81_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL1110665.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11244..11765
                     /locus_tag="PWU81_001973"
     CDS             11244..11765
                     /locus_tag="PWU81_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL1110666.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11844..12398)
                     /locus_tag="PWU81_001974"
     CDS             complement(11844..12398)
                     /locus_tag="PWU81_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL1110667.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12651..12926
                     /gene="rpsP"
                     /locus_tag="PWU81_001975"
     CDS             12651..12926
                     /gene="rpsP"
                     /locus_tag="PWU81_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL1110668.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12938..13189
                     /locus_tag="PWU81_001976"
     CDS             12938..13189
                     /locus_tag="PWU81_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDL1110669.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13313..14104
                     /locus_tag="PWU81_001977"
     CDS             13313..14104
                     /locus_tag="PWU81_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110670.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14274..14795
                     /gene="rimM"
                     /locus_tag="PWU81_001978"
     CDS             14274..14795
                     /gene="rimM"
                     /locus_tag="PWU81_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL1110671.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14785..15546
                     /gene="trmD"
                     /locus_tag="PWU81_001979"
     CDS             14785..15546
                     /gene="trmD"
                     /locus_tag="PWU81_001979"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL1110672.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15682..16029
                     /gene="rplS"
                     /locus_tag="PWU81_001980"
     CDS             15682..16029
                     /gene="rplS"
                     /locus_tag="PWU81_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL1110673.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16339..16581
                     /locus_tag="PWU81_001981"
     CDS             16339..16581
                     /locus_tag="PWU81_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDL1110674.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            16932..17186
                     /locus_tag="PWU81_001982"
     CDS             16932..17186
                     /locus_tag="PWU81_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HDL1110675.1"
                     /translation="MLELSKQLPVSDPRHFDYEEIAIKILEELQKNYTTKRVNGSNGL
                     LLHAVYDKNSLKGVDECVIWGDYFYVEGITRLAKTWYCYW"
     gene            17215..18474
                     /locus_tag="PWU81_001983"
     CDS             17215..18474
                     /locus_tag="PWU81_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL1110676.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            18527..19423
                     /locus_tag="PWU81_001984"
     CDS             18527..19423
                     /locus_tag="PWU81_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1110677.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            19434..20303
                     /locus_tag="PWU81_001985"
     CDS             19434..20303
                     /locus_tag="PWU81_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1110678.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            20313..20988
                     /locus_tag="PWU81_001986"
                     /pseudo
     CDS             20313..20988
                     /locus_tag="PWU81_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            21194..22858
                     /gene="alsS"
                     /locus_tag="PWU81_001987"
     CDS             21194..22858
                     /gene="alsS"
                     /locus_tag="PWU81_001987"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDL1110679.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            22871..23581
                     /gene="budA"
                     /locus_tag="PWU81_001988"
     CDS             22871..23581
                     /gene="budA"
                     /locus_tag="PWU81_001988"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDL1110680.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            23711..24208
                     /locus_tag="PWU81_001989"
     CDS             23711..24208
                     /locus_tag="PWU81_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDL1110681.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         8034 a         4142 c         5003 g         7070 t
ORIGIN      
        1 agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg tgaataagat
       61 aataaaattt gtatttgtcc tttttaggta acttatttta atctatatac ctttctggat
      121 caacgaaagt atactttata tagtcataac gccgatggta actatgcact gtaacgataa
      181 tgctgctaaa atatcttttt acgaaaaaca tcacaaggaa aaacttaact atggtattat
      241 ctaaacataa ttttttcggt atttaatatt taattaggag gtaatatttt gcttttttat
      301 agtaatttta tcttaatagt agcgatatta ttacttctga atatatggat ttttgatagg
      361 agtagaaatg caagtatagg ttttagaact aaacgaagtc tgtcttctaa aaaaaattgg
      421 gtatactcac aaactatttt ttatggagga attgttttaa ttagtttact ttcttcaaca
      481 ctttattctt tgaatattat agatgtttcc acctcaaatt caatatctat tattggaatc
      541 attattgctg caattattac acaactgttt ttagtgtttg gagagaagaa aagaagtaaa
      601 aaatagaaaa actagtgata gttttggtga tataagaaaa acatatatgt ataaaaagaa
      661 gaaagtagct tctctttgta catatatggt ttgtttttat ggatgccgag cttttttata
      721 agataaggct accgcccata acgacccaag gatttgtgga actaagtaaa tacctgcaag
      781 aagcaagatc agctgggtcg gttttaaagg atgatagaag taacgccatt cagtaaaatt
      841 ggcgcaaaat aaataggaag tgacagtaga tataatattg gcaatgaata aagatataat
      901 acgcctgttc ctttgaaaat aatatccagc aaaaagagat aaaaatatca atgtgatgaa
      961 cccgcttacg gaatgataac gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa
     1021 agataaaagc aacggaaggt ttttccagca agtttttatc atctttcatc accacctaac
     1081 aagtgttatt attttgttag ttttattgta aattgaatcc tgcaataatc aattactttt
     1141 tcattatttc tagattaaat gaataaactc tcatgccatt atctctaaaa aaaaagtttt
     1201 tccccctaat ttttttagat agaaaattgt atagtatgat gagagaaaaa gtagtttggg
     1261 ggaacgttaa tgagaagaaa agcagtaatc gatcggcttt atttattttt ttggtttttt
     1321 cctatcgttt tgctaccttt tacatgggta aatgctgttg tacaaggtgc ggagatatta
     1381 ggcctgatcc aaggaagcgg attagtggtt tctaggaatt ttccagcttt gactgttttc
     1441 tatttagtga taagtgtagg tctcttatta ccaaaatctc tgatcaatca ctacaattgg
     1501 cagctgcttt acttttcttg cttattagta tttacatcta tgttttcttt gctgcctcgc
     1561 ttgattatcg gccctaatgg tttgagcgag agtggattga aggattcagc ttattcctcc
     1621 atgtatttct tgacgatcaa accatggttc tatttagtaa ttgcctcatt tattcttgca
     1681 acattttttt acttctggac agaaatcagg ataaaaagtg aacgatctgt atctataaaa
     1741 aataactatt gatggtggat catcagtaaa aaagcatatg agctgcgtaa aatgcatgtt
     1801 tctcatatgc tttttttatt attatgtaaa gaatgcgaaa aacgtttaca tgatttttac
     1861 ataacttaac tatattttta cagcaaccat acgagggatt tacagtcgac tgataaactt
     1921 tagttgtaaa taagaaagga gcaataaaat gaaaaaagta ttgtttactc taggtatgat
     1981 gggaattctt ttagccggat gcggctcagg atcaggaaat acagatggct ctgccacaca
     2041 aaataattca agtgactcaa atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt
     2101 acagccgtta gtcgatgcag cacaagaaag ctttgtacaa gaaaatccaa attatcaaat
     2161 ctctgtacaa ggaggaggaa gcggaactgg tttgagccaa gtcgaagctg gagcagttac
     2221 tatcggaaac tctgatgtat ttgcagaaga aagagacgga gttgatgctt caaaattagt
     2281 ggatcataaa gtagcagtgg tcgggatggc accaatcgtc aataaagata cagatgtcaa
     2341 agatatcacc aaacaggaat tgattgatat ctttacagga aaaatcacta actggaagga
     2401 agttggcggg aaagatcaaa aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg
     2461 cgcgacattc gaaaaatggg gactggatgg tgctacccct gtgcagtccc aagaacaaga
     2521 ttcatcagga acagttcggc agctggtaag tcaaacacca ggagcaatca gctatttagc
     2581 tttctcatat ctcgatgatt ccacacaagc actaagtatc gacggtgtag aaccaaaaga
     2641 agagaatgta gcagataaca gctggggaat ctggtcatat gaacatatgt acacgaacgg
     2701 aaaaccttcc cctgaagtcc aaaaattctt agactacatg atgacagaag aaatccaaga
     2761 aggacctgtc aaagaattgg gctatttacc aatcacaatg atggaagtag aacgtgatca
     2821 cgaaggaaat atcaaataac tagcagataa acatgccacg gtagtcgtta ctattaagga
     2881 aaaatcaact atgtttcccc acatatacat ttttccttta attgtgaaaa atagttcctc
     2941 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttattcga
     3001 aaaaaagagc tacgacatag aactgtcgta gctttttttt tgctatatga caggaatgaa
     3061 aggtgtttgc aagcttaatg tgaggaacac tttccatatc tgttctatac tggataggag
     3121 aagtttggaa atatccagaa aacgaaatca atcgaggtag ccacatgaag aataaatgga
     3181 taaatcgaag taaaaaaaag aaagtaacac aaagatcatc agataaagtc atccgtcttt
     3241 ttccatcgta taaagaagga ttaacggcag atcaagtcaa ggaacgaata gaaaaaggtg
     3301 cagcaaataa ttcagtcgat cccacattca aaacaaatca gcaaatcgta ttggaaaaca
     3361 tatttacgta tttcaatttg atctttctta tcttagccat tttgctttgt ttagtagaat
     3421 cctataagaa cttgactttt ttaccagtta tcatagccaa tacaggaatc gccatttttc
     3481 aggaaattcg atccaaaaag atcttggacg aactaaatgt tttaaataca gcaaaagtca
     3541 atgtcatacg agacgggata gaacaaacaa tagatataga agaccttgta atagatgaca
     3601 ttgttatttt agagactgga catcaaattc cagcagatgc agaggtcgtg gaagggaaac
     3661 tgcaagttaa tgaggcttta ttgacaggcg aagcagatga aataaccaaa gaaataggcg
     3721 atccattaat gtccggcagt ttcattgttt ccggaaaagc ttacgcccga ttagacaaag
     3781 ttggagcaga ttcgtatatt tcccagctga cagtcaaagc caagacaatg ggagaaggag
     3841 aacagtcgga gatgattgct tctcttaatc aattgatcaa atggattggg atcatcatta
     3901 ttcctatcgg tatcctgctt ttttctcaaa gttacttttt taatggaaac acgatcaaag
     3961 aaagtgtcgt tgcaatggaa gcagcattga tcggtatgat acctgaagga ctgtacttgc
     4021 tgacgacgat tgcattagca ttaagtgcga ctaaactagc aaagcaaaga gttcttttac
     4081 acaatatgaa aagcattgag acattggcaa gggtaaatgt cctttgtgta gataaaacag
     4141 gaacgatcac agaaaataaa atgtctgttc aaaaagtgat cgtctctaaa aaacaagaaa
     4201 taatcagtca gtcagatcaa ttggaagcat gcattgctga ctatgcaaaa gcgatggcag
     4261 gagacaatgc aacaatggaa gctgttaaag catattttac aaaaaccact gatcaatcct
     4321 atcaccatgt tattcctttt tcttctgtcc aaaaattcag cagtgtagcg ttggcagaca
     4381 aggtgtatgt tttaggagcg cccgaaatgg ttttgcgcga tcaattgccg gaatatgcgg
     4441 aagagtttgt ttctttcgct gaaaaaggct atcgcgtgtt ggtctttggt gtgtacgatg
     4501 ggattcttga agaaccagta ttgacagaag cagtcacacc attaggctat attctgattg
     4561 ctaatcctat cagaaaagaa gcaagagcca catttgatta cttcaaaaaa cagcaagtag
     4621 ccatcaaagt gatttctgga gataatccgc tcaccgtatc caatgtagct gtccaagcag
     4681 ggatttcaca tgctgatcag tacgttgatg catctaaatt atcagaagaa gaatatgaag
     4741 cagctgtcga aaaatataca gtttttggac gcgtcaagcc agaacaaaag aaaatcttcg
     4801 ttcaattgct aaagcgtcgt aacaatacag tagctatgac aggtgatggc gtgaatgata
     4861 ttctagccat gaaagaagca gactgcagta tagcgatggc ttccggaaat gaagccgcca
     4921 tgcaggcctc acaagtggtc ttacttgatt ctgacttttc acgcatgcca gaagttgttg
     4981 cagaaggacg gcgcgttgtg aacaatattg agcgatcagc cagcttgttt ttagtgaaga
     5041 atattttctc ctttttgctc tcactgtttt cagtgatctt tgctctgacc tatcctttag
     5101 aaccatcaca aatcacattg attagtttgt ttactatcgg acttccatct ttcttgcttg
     5161 ctctagaaga aaacaaaaag cgaatcagag ggaaattcat catgaatgtg atggaaaaag
     5221 ctgtgccagg tggactaaca gatatgatcg tagtcggtgc gttggtgatc tgcggggtga
     5281 ctttgaatct aaataaaaca gatgtttcta cagcatctac catgcttttg attgcagtag
     5341 ggttcctcgt tctctataaa atctgttcac cgctgaataa attcagaagt cagatcattc
     5401 ttttctgtgc tagcggtaac tttttttcgg tcatcttttt gcataagctg ttttcaatca
     5461 ccggtatctc agctgtatca cttttgctgt tgagcattct atttttctcg gcagattcga
     5521 tttttaggca tttgaccaca ttggtagaat atttgtttga acgtaaaaat gaaaaagagc
     5581 aaaagccgtt tagtcgcgta gttaaaaaat ttctatcgat atttaaacga gaaaattgac
     5641 gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt cgaagcagga
     5701 tgagtcatcc aactttaaaa cgcacgagca gttgctcttt tttacatttt cggaatggta
     5761 acggtgaaag tcgaacctgt tccaagatga ctgtctacag taatcgtacc atgaaggagt
     5821 tgcacgtagt cttttacaat cgctaaccct aatccggtcc cgcctgaatg ccggcttcgc
     5881 gccttgtcta cgcggtaaaa acgctcaaag atccgttcct gatcctcaat gtcgatacct
     5941 ataccaaagt cttggacaga aaacgataga tcatctgttg cctgatagcg gataataatc
     6001 tcgctgtcgc tttttgaata ttggacagca ttttcaatca gatttttgat aattggataa
     6061 aggatatctg ttttagtagt aaatggttcg ttctttccaa tcaatcgaat cgtcaaccgt
     6121 ttcttttcga tgattggctg ataattttga acgatttgct ggaaataagg ttctgtataa
     6181 attgtctgga tctcgtaaga attctcgcca tcttttgaca gctggatgat ttcccgaatc
     6241 agtttatcta accggatcgc atctttttgc atgatctcaa gaaaagaagt cagagtttgc
     6301 gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac caatcagtga agtcacagga
     6361 gtttttaatt cgtgagagac gttgccaacg aaatcctttt gtagcttttc taaacgtcgc
     6421 actttcgtta agtcatacgc cacccctaga atctgtccgg taccttctga attagtaaag
     6481 tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa gggtgatctc ctgatggatc
     6541 agcggatgct caggagtcac ttgatgaatc agctgaatga gttttggctc ttgaatgact
     6601 tctgtgtacc gctgttcagg ttgatagctt accacgttta aatgtagctg catctttgga
     6661 ttaagcagta gtaaacgaga atcagcatca tcaatcagga atacgccaat catcagttcg
     6721 ttcagtaacg tatataattg ctcttctgtg gtagtatacg cccgatacat cttactcatt
     6781 tgttcgctca acgcattgat cgtctgatac aattcttccc attgatcggg tgtttgcatg
     6841 attacttccg cctgattagg attttgcacc atttttttta gtacgggcag cacggttttc
     6901 aatggctggt ttttttgata gatcagataa tagatcattc ctgcaagaac aaggaagaaa
     6961 gacagaaaga agaaaaagac ccaacggcga aaactctcag ttctcggtaa aaaaccgctt
     7021 gtcggttctg cgatacgtaa aatggcttct aactggccac tttttttgac tggtaatgcg
     7081 acatataaaa gatccccatt caaagtagta cttttgcgca aagcgctacc taggttccca
     7141 ccattcaata cggctttgat ttcgggacgg gagtttcgct gttcattcaa tgtttcattg
     7201 tttgtgtcaa ataagatctt tccagtagca tccattaaag taatccgttc gttcgactga
     7261 tggacgaatt cttccaaagc agttttgttt tgcgctgctt ggaggttttc gacatctaat
     7321 tgccggatca gcagttgtgc tttttttgtc aggtagcttt cttgctgttc cacgatctgc
     7381 tgttgaaaat aatgggaaat caattgccag ccaccaaaaa acaaactaac taaaagaata
     7441 agaaagaaga gacgttcata aatttttttc catttcatcg tttgggttcc tgaaacttat
     7501 agccaaatcc tctaatagtc accaagtact ttgggtgttt tggatctcgc tcgatttttt
     7561 ctcgcaaatg actaacgtgt acatctacga ttcggctttg tcctgcgaag tcaaaattcc
     7621 aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc ttttcgtttc atgaaataaa
     7681 caagtaattc aaattctttc ggtgttagtt caatcagatg gtcgtctacg gtcacttgat
     7741 aattggtcaa atcagcagtg atttgtccaa tcgatagata ttcaggctca gcttcatctt
     7801 ttgattgctt acgaggttca attcgtctga atattgcttt catacgggct aatacttcgc
     7861 gaggactaaa tggtttggtc aaatagtcgt cagcacctat ttctaggcca atgatccgat
     7921 ccacttggtc atctttagcc gtcagcatca aaatcggtgt ttcatttttt tcttgacgca
     7981 gccgctgagt gatctccatg ccatccatcg ttggaagcat cacatctaaa atgatgaaat
     8041 caaatgattg ttctaatgct aattctaatc cttcgcctcc atctgtggca ctggttacct
     8101 tgtagccttc tttttccaaa ttgaacgtca agagcgttac aatagaaggt tcatcatcaa
     8161 caactagtat ttttttcatt caaatctcct ttaaataggt ttcattctgt ttatatttta
     8221 gcatagaatc agtacaaagg gctattttac aaaagaaata aatggtataa taggaaggca
     8281 ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat cagtgcatgt ttaggcggtc
     8341 tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga attgatggaa ctagttgaaa
     8401 ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg attaccaatt cctagagatc
     8461 ccgctgaaat cattggtgga aatggattcg atgtgtggaa agatcaagca aaagtatgga
     8521 cgacaagcgg cgaagatgta acggaagcgt acaaaaatgg cgcgatcaaa gcctatcaaa
     8581 aattgcagga aaaacacatt gatctagtca tcatgaagga aaaaagccct tcatgcggaa
     8641 cccattcgat ttatgacggg agtttttccg gaataaaaaa aaatggtgcc ggagtggcta
     8701 ctgcttattt tattcagcaa aaaatgactg tgctctctga tgaagaatgg ctgaaaacta
     8761 gaggtgagca gaatggaaat cgaaaagacc aatcgaatga acgcgctctt tgaattttat
     8821 tcgaccttat tgacagaaaa acaaatgaac tacatggaat tatattatgc agatgatttt
     8881 tcattaggtg agatcgcgga agaatacgaa gtaagccgac aagcagtata tgacaatatc
     8941 aagcggacaa gcaagatctt ggaggattat gaaaaaaaac ttcatctttt ttctaattat
     9001 attgttcgag aacaggtatt ggaaaatatg actgcatata ttacagataa gtacccggaa
     9061 gataaaaaac tacttgaata tgtacagcaa attcaagcaa tcgaagaata aataaaagga
     9121 atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct ccagcaggcg atgggaaaaa
     9181 taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga aatgatgcgg gaaatccgcc
     9241 ttgccttact agaagccgat gttaacttgc aagtagtcaa agattttact aagcgcgtac
     9301 gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc acctgcacaa caaatcgtaa
     9361 aaatcgttga tgaagaattg acgatcacac ttggttctga aacagcagag ttaaataaat
     9421 caccaaaaat accaactgtt atcatgatgg ctggtcttca aggggcaggt aaaacgacct
     9481 ttactggtaa attagccaat tatttaaaga aaaatgaaaa tgctcgtccg ctcctaatcg
     9541 caggggacgt atatcgacca gctgcgatcg accaattgaa agtattaggt caacagctag
     9601 acgttcctgt atttgatatg ggaactgaag tcagcccggt agagatcgtc cgtcaaggga
     9661 tggagttagc aaaagaaaag aaaaacgact atgtattgat cgatacggca ggacgtctgc
     9721 atatcgatga aacattgatg gatgagttga aacaaatcaa agaattgacg caaccaaatg
     9781 agattttgct tgttgtcgat gccatgactg gtcaagatgc cgtcaacgta gctgatagct
     9841 tcaaccagca attaggtatt acaggggtcg tcatcactaa acttgatggg gatactcgag
     9901 gcggggcagc actatcgatc cgctcagtga caggcgcacc aatcaaattc atcggttctg
     9961 gtgaaaaatt gactgactta gaagtattcc acccagatcg tatggctagc cgtatccttg
    10021 gtatggggga tatgctgaca ttgattgaaa aagcgcagca agactatgac gagaaaaaag
    10081 cggaagaact tgcacagaaa atgcgggaaa attcattcga tttcaatgac tttattgaac
    10141 agcttgatca agtgatgggt atgggcccac tggaagatct aatcaaaatg atcccaggaa
    10201 tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc aaaagatgtt gaacgcaaaa
    10261 aagcgatggt ttattctatg acgccagcag aaagagcaaa tcctgatctg ttgaacccaa
    10321 gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt tgttgaagtg aatcgaatga
    10381 tcaaacaatt caaggaatca agaaaaatga tgcagcaaat gtccaaaggg gatatgaaca
    10441 tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg aaaactaggc aaaatggcga
    10501 tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa acggaaataa gatgaaaaaa
    10561 acagcttcgg tacagactga aaatgtctac tgccggagct tttctatcag caatttttat
    10621 tctttactaa tacaattact ctaaacgaag aggtggcaca gatgagatgt ccgtggagtg
    10681 aacaaacaga aagtatgcgg caatatcatg atactgtctg gggtgttcct gaatatgatg
    10741 accaaaagct gttccgaaaa ttgatgcttg atattaatca agccggattg agctggcaga
    10801 cgattttaaa taaaagtgaa gcattcgatg aagcatatga tcgattcgat attaaaaaag
    10861 tcgcaaatta tggagaaaca aaaatcagtg aactaatgga aaacaaagga attattcgta
    10921 accgaagaaa aatcgaagca gccgtccaaa acgccaaagc agtcttgaaa attcaagaag
    10981 aaatcggcag tttttcagag tatctttggt cgttttctaa tggaaaaatc gttgattctt
    11041 cttatcattc acaagaagaa gtcccaacaa caagtcctct ttcagatgaa ttagcaaaag
    11101 atctaaagaa aagaggattt aaatttatcg gaagcacaac aatctatgct tttttagaag
    11161 ctgtcgggat catcaatgat caccttgaca gttgtttccg caaagaagag gtcgtacgta
    11221 tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt ttatggtgct ataagtttag
    11281 atggctattt ggcaacgaaa acggatgatc ttcaatggct ttttgacact ccaacaggta
    11341 agcaaaccac ttatgaagct ttttatgaaa caatcgatac gaccatcatg ggcagaaaaa
    11401 cttatcagga agcaaaaaaa tatttggaga ccgaagcaat ttatccagag aaaaagaatt
    11461 tcgttttttc cacccacgca caattacaat tgccagatgc tgaggtcgta aacgaagatc
    11521 ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa agatatctgg gttgtgggtg
    11581 gcggtcgatt gatcaaaccg ctattggaac atcaaatgat tgatgaatgg tatatccaga
    11641 ttacccctgt attattaggg gacgggattc ctttattcca aaaaggaaca tatgaagcgc
    11701 gttttgaaat gctagacacg acacgattcg gtgaatttat cgaattgcat tatgtacgaa
    11761 agtagaagta aataaaataa aaaaggcaac tatatgcaga aatgtcctgt acatagttgc
    11821 cttttttatt accgttatgc tttttactct gttaatttgt tagaaaaatc tagatacgca
    11881 tctaaaatcg tttggaagaa acttaatgtt ccttcaacga tattaccatt ttcatcaatt
    11941 agattcgcaa cgtttccgat atatgcttcc ggttgctgca aggttggcac attcaaaaag
    12001 acaagtgatt gtcgtagatg gtggtttgcg ccaaagccgc cgatcccacc tggtgatgca
    12061 gtaacgacta aacctggttt atgatcccaa acactgtgtc cataaggtct tgagcctaca
    12121 tctaaagcat ttttcaatac tgctggcaca gaacggttgt attcaggagt aacgaaaacg
    12181 acaccgtcta gtcccctcac ttcctcacga aaacgagtcc attcagcagg agcctgttct
    12241 ggagtttcga gatcctcatt gtaaaatggc agatcatcga tttttacaaa tacactttcg
    12301 tacccttcag gaagcaattt cgcaaaagct tctgctactt ttttgttgta agaatctttt
    12361 cttaaactac caataaaaaa tccaattttt tttgtcatac gatttttcct ccttcaaact
    12421 atctataaca atcttacaaa aaataagtaa ctggagcaaa taaaaacact tactgacaaa
    12481 agctacatag aagatagatt acttattttt aacaaaaaag aagtctaaaa tcttctattt
    12541 cctaaaaaaa atcgaattta atcaagctgt aaagaaaaaa agctttacag ggtaaaaaaa
    12601 aactgttaca ctacatcttg tgaataaaac aatggaggtg gaaataaata atggcagtta
    12661 aaatccgttt aaaacgtatg ggttctaaaa agagtccgtt ttaccgtatc gtcgtagctg
    12721 attcacgttc tcctcgtgat ggacgtttca tcgaaactgt aggtacttac aatcctttga
    12781 aagaccctgc agaagtagtt ttaaaagaag acttagttct tgactggtta tcaaaaggtg
    12841 cacaaccttc tgacactgta cgtaacatcc tttcaaaaga aggcgttatg caaaaacacc
    12901 acgaagctaa attctcaaag aaataaggtg accggatatg aaagatttaa gcgacttagt
    12961 cttaacaatc gttcgtccgc tagtcactta tcctgatcaa gtcaagttag acgtcgtaga
    13021 gtcgaaagac ttttacgagt acaatctaac agtggctcct gaagatattg gccgtatcat
    13081 tggaaaacaa gggcgtgttg ctaaagcaat ccgcacaatc gtttacggtg ttcggatcaa
    13141 tgcaccgaaa aaagtgcgtt tgaatatcat cgatgataag gaaaagtaaa taaacgtgct
    13201 gtactcgaat tttcgagtgc agcttttttg tttttttatt aattgaagaa atgattattt
    13261 ggcaaaacaa gcaagaaagc gtatccttat aggaggaggg agtgaacagc caatgagaaa
    13321 gaaaatgtta tttattccat ttgctctatt gttaagcgct tgtagttcgg ttactcaaga
    13381 atcagacaat acacatctat caagcagtat atctgaaact gttaccagtc cagaaaaaag
    13441 tagtaccaaa acggctacca ccactgaaca aacaatggct acctcaaata atgagaaaaa
    13501 gactgcttta gaccagttga aagaacaaca gccaaatgtt cccatgcctc tagatgtacc
    13561 agtctcttca ggctatctca atatcgcagc gacacataca aagcaaggtt attctatttt
    13621 atattacaga acagatcgtc ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc
    13681 tattgcaacc tatctatatc aatatgggtt tgcttctagt caagaaacaa tccaagtttt
    13741 gcagcccttt gagatcgaca caaatggtca gcaagtggac ttaggttctc gaatcacagg
    13801 gtatcagcaa ggtgcagcag gttcaagttt tcttgaatgg caagaaggaa actggcgtat
    13861 tcgaatccgt gggaataata tagaaggcca agatccctta ctattagcta aagaaatcgt
    13921 agcttatttg gaagaaaaca gtctaccagc acctgaacaa tttggtaaaa ttactgttga
    13981 tatgggtgat acgacgaatc gagccgtaga agtaagctgg caagaaccga aaaatgccta
    14041 tacgatcact catcaagatc ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt
    14101 gtgaaatcag cgcgctgatc tgagcccatc atttaactcc aagtcacagg aagtaatcaa
    14161 aaatagcacg ccgcattttt gcataatttt ggcttaatac cttaaaagaa aactagaatt
    14221 gcctcttcac acatgctaaa ataaaacaga caaaaaagaa atggggatat aagttgactg
    14281 aatacttgaa tgtaggaaaa atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta
    14341 tctcaacaac ggatttccca gaagaacgtt acaaaaaagg aacggtgtta acactattcc
    14401 aagacggaaa agcacctgtg gaattaacag tcaaaagtca tcgcaaacat aaaaattttg
    14461 acttattgag ttttgaagga catccttcta tcaatgatgt ggaaaaatat cgggatggca
    14521 tattaaaagt gtcaaaagac aactctgtag atctagctga aaatgaattc tattaccatg
    14581 agatcatcgg tctgcatgta ttagaagatg gaactgagtt aggtaaagta aaagaaatct
    14641 tgtcaccagg tgccaatgat gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc
    14701 ttccatatat cgcttctgtc gtcaaagaag tcgatttaga agctggagtg gtccgtgtag
    14761 aaattccaga aggactgatt gacgatgaag attgatgtat tgactttatt tccaagaatg
    14821 tttgaaggtc ctatggggga atccatcatc ggtaaggctg tagataaagg attattagat
    14881 atcaatatct ctaacttccg cgattattca gataacaaac atcagacagt agatgattat
    14941 ccatacggcg gtggagcagg tatgctgctc aaagtccagc ccatctatga taacatccaa
    15001 gccattgaaa aagctgcacc ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt
    15061 aaacgattcg atcagaaaat ggcagaagaa ttttcacaag aagaacactt agtattcatt
    15121 tgtggtcatt atgagggata tgatgaacgt atccgcacat tagtgacaga tgaagtttcc
    15181 ttaggagatt atgtccttac tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca
    15241 gttcggcttt tgccagatgt gttagggaat cagacttcag ctcaaacgga ttcttattca
    15301 accggtttat tggaacatcc ccaatatact cgcccggcag aatataaagg gatgaaagta
    15361 ccagaagtat tgacaaatgg aaatcataaa ttgattgaag aatggcaatt aaaagaatca
    15421 ctaagaagga cctatcaacg tcggcctgat cttttggaaa cacttgaatg gacacctcaa
    15481 atgacgaaat ttttagaaga aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc
    15541 aagtgacttt ttctaaaata ttctttacag taggttgata atatgttagt attttatggt
    15601 gagtgcaaaa gcacttaact attacgatat tccgctatga gaaaagctca taagaatatt
    15661 tggaataagg agaagaaaac catgaatcca ttaatcgaag aattaacaaa agaacaatta
    15721 cgttctgaca tcccagcgtt ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt
    15781 gaaggtactc gtgaacgtat ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct
    15841 ggaatcagcg aaacttatac agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca
    15901 ttcccattgc atacaccacg tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt
    15961 cgtgcaaaat tgtactacct acgtgcatta cacggaaaag ctgctcgtat caaagaaatc
    16021 cgtcgttaat cattgttatc agactgagaa agaacccttg gtattcaagg gttctttttt
    16081 cttagcatgt aatttgaaca ccctgatcca gtttttttct ttaattattg gtaggggaga
    16141 ataatggatt agaataaatc tacctataag tagtagtaaa aaaaagagca ctatgataat
    16201 atctttctta agagtaaccg ctttaataaa aaatccagaa tattctaaca taaatggagt
    16261 attcttagaa tcaaaaggag aaagaagatg gtttatactt atacggagca agaaaaaatg
    16321 aaagtttgag acacattcat gaatgatgga aaagtaaaac aagtaccaag ttcaacaaaa
    16381 aagaaaaaca tcctattgaa agaagtcttg aaacgctttg accatggagt gacttataca
    16441 gaaacagagg ttaattcgat actgctgaac gtattttcta gtggcgatta tgtcgaacaa
    16501 agacgttatc taatcacatt tggctttttc aaaagaagtt cagacggtcg ggcatatcag
    16561 atgatgggaa ttgaaaatta gaagttttaa tttatactca ttgaaaaaag ttcaagtatg
    16621 cgatattggt tctacaccgc atacttgaac tttttttgtc atctgtatct ataatacaaa
    16681 gccaagaacg ttattcaaaa tatggatagc tatgataaaa gctaatgagc gattcgcagt
    16741 taaataagca atcgtcagta aaatcgttcc aggagcgtac atgaaaaaat caattggatg
    16801 cgttacatga ataaagataa ttctctttta tttgtacgaa taaataccaa aacgtacctc
    16861 ctaaaattag attcaaagaa gatagcaaag aggaaaaaga tagttctgca gcagctatag
    16921 cggcatgtgg attgctagaa ctttccaaac aactgcctgt ttcagatcct cgtcatttcg
    16981 attacgaaga gatagcaatc aaaatcttgg aagaattaca gaaaaactat acaacgaaac
    17041 gagttaacgg atcaaacggt ctgcttctcc atgccgttta tgataaaaac agcttaaaag
    17101 gtgtagatga atgtgtgatc tggggcgatt acttttatgt agaaggaatc actcgtctag
    17161 ctaaaacatg gtactgttac tggtgatgta taagagaaga ggaggattca tataatgaag
    17221 cgatggaaaa aaatgatgat tggatcaata gttttaggaa tgtcactcgt atttggtgct
    17281 tgtggtaatc aagaagacag tagtgaagaa tcagcaaatg aaattaccgt ttcgacatgg
    17341 aattatgaaa caacacctga attcaaagcg ctctttgaag cattcgaaaa gaaaacggga
    17401 atcaaagtga aagcagtaga tattgcttca gatgattatg atacgaaact caccacgatg
    17461 ctttcttctg gagacacgac agatgtattg acgatgaaaa atcttttgtc gtattcaaat
    17521 tatgctttac gagatcagtt agtcgatcaa acagaacgaa ttagagaatt agacactgat
    17581 gctgctgaag aaacatatga aatgtatgat atcgatggaa aaacatatgc attgccatat
    17641 cgaacggact tttgggtact atattacaat aagaaaatgt ttgatgatgc aggaatcgaa
    17701 tatccaaaaa atctaacttg ggacgaatat gaagatttag caaaaaaact tagtaaaaat
    17761 gacggacaag tttacggagc atatcaacat atctggcgtt cgacaatcca agctattgca
    17821 gctgctcaaa acgaggcgaa tcttgttgaa ccagattatc aatatatgac tgattattat
    17881 gatcgagcat tgaggatgca aaaagaaggc gcgcagatgg attttggcac ggctaaatca
    17941 acgaaagtaa cctatcagtc tcaatttgaa gaacaaaaag cagcaatgat gtacatggga
    18001 acttggtata tggctggcat actcgcaaac aaagaagcaa gtaagacaga agtagaatgg
    18061 ggaattacag ctattcctca gaagaaaaaa ggagaatcgg tcacgacgtt tggctcacca
    18121 acagcatttg ctgtcaataa aaactcaaag aaacaaaaag cggcgcaaga atttattgaa
    18181 tttgcagcca gtgaagaagg agcaaaagta ttagcaggag taggagtagt tccttcatat
    18241 cgtacggatg agattgatca gctttatttt aatctcgctg gaatgccaac ggatgaaatt
    18301 tctaaaaaag ctttttctcc tgatgaaatc aaacttgaat ttccgattga tacgtatggg
    18361 cctgcaatcg ataagatttt acaagaagaa tatgacttag ttttggttgg agatgaaacg
    18421 ccggaaaaag gaacggtaaa tatggagaaa cgtgtagctg cggaaaaaga ataatcatga
    18481 aaggagagaa gccaagagat aaaacactct tggtttcgtc atcgaaatgg agaatagtat
    18541 aaataagaaa gctacaaatc atttgcgtag aaaacgaacg tggatcgctt attcgtttat
    18601 cgccccgaat ttcattggtt ttttcgtttt tacattaata ccaatcctgt tttcgcttgt
    18661 actagcattt atgaaatggg attctttttc aacgccagaa tttgctggac tagataactt
    18721 tcgtaaaatg gccaacgatg agacattttg gatttctttg aaaaatactt ttttatatac
    18781 cgctgctgta gtgcctggca cacttgtttg ttcgttaggg ctagctttac tactaaatca
    18841 gaaaattaga gggatgaagc tttttcgtac gatatttttc tttccgtatg ttacttcctt
    18901 agtagcaatt gctgttgttt ggaatatgtt gtttcatcca acagtgggac ctattaatat
    18961 ctttttaaga cgatttattg aacatcctcc aggttggact tcaagttcaa attgggcgct
    19021 ttgggcaatt gtaattgtca gtatttggcg agggatgggt tattacatga ttctatacct
    19081 tgcaggacta caaagtatcc aaaaagaact atatgaagcg tcttcgctag atggagcaaa
    19141 ccgttggaaa caatttttga atgtgacatt accttcgttg agaccaacaa ccttctttgt
    19201 aacgatcatg cttgtcatca attgtttcaa ggtgtttgac cttgtgcaag tgatgacgga
    19261 tggcggccct ggtcgagcga caaatgtgct ggtttaccaa atctacaatg aagcattcaa
    19321 aaaatttgat tttggttatg cttcagctat ttcacttgtt ttgttcgtaa ttgttttggg
    19381 cgttacggtt gttcaatttt cttggaataa gcgtcgtact taggaggtgt gatatggaag
    19441 agagtgttcg aaatgaaaga aaaaaagcaa aaattcgttc aaaaaacatg ccctttattc
    19501 aatggttggg tatgattctg ctgattgtat tagctttatt gacgatcatt ccttttttat
    19561 ggatggtttc tgcttcattc aaagcaaata atgaagtgtt aacgattccg attcaatgga
    19621 tccctaaaac ttggcattta gagaattatc tggttatttg gtctcgtatt cctttagtga
    19681 cattttttaa gaatacagct tttttaagcg ttgtgatcac gttgattcag ttgtttactt
    19741 ctagttttgc tgtttacggg ttttctgaac taaattttaa agggcgcgat attttatttt
    19801 tagcatatat cggcacgatt gctgtccctt ggcagtctta tatgatcccg cagttcatta
    19861 tgatgagaaa agtgggttta acagatacgt tgtggtcgtt ggtgctatta caagcgttca
    19921 atgcttttgg tgtgttcttg ttgaagcaat attacagtag tatcccagat tctttatgtg
    19981 agagtgcgcg gatcgatggc ttatcagagt gggaaatcta tcgcaagatc attttacctc
    20041 ttacaaaacc ggcgattgcc agtttgacga tcatcacatt cgttaataca tggaacgatt
    20101 atatggggcc attcatttat ttgtcttcta cggaaaataa aacaatccag ttagggttga
    20161 aaatgtttat tgggctattt gatgcagagt acgcattaat catggctgcc tcagttgttt
    20221 ctgttcttcc tgttgctttt atatttttag cgatgcaaaa atattttgta gaagggatcg
    20281 cttcatcagg aatgaaggaa taggaggtga ttatgaaaga gcagcgtttt gacaaaaatg
    20341 tttatatgga attcttttat tggttgtatc aatcattggc aagtagcctt tgtttttggt
    20401 gtgtcaatat cttgtatata gcagctgcgt tccttttgtc gatagatgga aggaatgtct
    20461 tttcctttat tttttcttta ctttttattg gtccaggttg catcgtgatg ataggtatga
    20521 cagaagaatt agcaaaagaa aaaagttttt caccagtacg tttgttcata aagacgtttc
    20581 gccaattttg gctaaaggga tttttttatt ggctatttgc ttggatagtt agtgtgataa
    20641 tgatttttga ttgtttcttt tttatacgtt tttcttatgg aaaatggttg attccgttgt
    20701 ttgttttact tgcttgtttt acttgcttgt ttatcagttt cctttagcat caactgctgg
    20761 tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat agcgttctac
    20821 tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac aagtttgttt
    20881 ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat cgtatttggt
    20941 ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac tttataaatt
    21001 gaaactttca attaagaatg acagtttttt atgtaaaaac tgtcattctt tttcattttt
    21061 tgaaacatat ggctaaacaa atgaaataaa acatgctata cttttgaatg aaacaggttt
    21121 agaaactgta tcacttattt gtgattattt ctaactaata attaaaaata aagcataaaa
    21181 aggacgatgt tcaatggctg aaaaaatgaa acaaggttcg aaatctgtag tagaaagttt
    21241 gattaatcat catgttgatt atgtattcgg tataccaggt gcaaaaatcg atggtgtatt
    21301 taatgaatta gaggatcaag gtcctgaatt aatcgtgact cgtcacgaac aaaatgctgc
    21361 atttatggca caagcaatcg gacgtattac aggagagcca ggagtagtta tcgcaacaag
    21421 tggtcctggt gctagtaact tagctacagg tttggtaaca gcgacagcag aaggtgaccc
    21481 agttttagca attgccggac aagtaaaacg aagcgatcta ttgaagttga cccatcaaag
    21541 catggataat gcagcactgt tccagccaat cacaaaatac agtgcagaaa tccaagatcc
    21601 agaaacaatt tcagaagtga ttgccaatgc ttatcgtatg gctaagagtt caaaaaaagg
    21661 cgcgagcttt atcagtatcc cgcaagatgt agtggatgca cctgtccaag gtaatgtcat
    21721 caagccattg agcgatccta aattaggttc tgcttctgca gatgatatcc gttacctagc
    21781 tgaacgcatt agagaagcaa aactaccggt actattagta ggtatgcgag gatcaagtga
    21841 aaaagaaacg ttagccatca ggcaattagt tgaaaagaca gcattgcctg ttgtagagac
    21901 attccaagca gctggagtga tctcacgtga attagaagcg catttctttg gtcgtgtggg
    21961 tttatttaga aaccaaccag gagatatgct tttgaaacga agtgatctag ttattgcaat
    22021 tggctatgat ccaatcgaat atgaagcaag aaactggaat gcggaaaaag atgcgcggat
    22081 cattgtgatc gatgaagcgc cagctgaaat cgatcctttt atgcagcctg aaagagaatt
    22141 gatcggtgat atctcagcca ctcttgattt attgacaggt tcattagagc ctcaacaagt
    22201 gtcagaagat gcaaaagaat atttagcatc tcttcaagca aaattaacgg agcgggacat
    22261 tgttcaatca aaaggagaag caggtatctt gcatccttta gaagttatta acacgcttca
    22321 gtcaaaagta acagatgata tgactgttac cgtagatgta ggtagtcatt atatttggat
    22381 ggcacgccac tttagaagtt atgaacctcg tcatttgtta ttcagtaatg gtatgcaaac
    22441 gcttggtgtt gcattgccat gggcgatttc tgctgcattg gtgcgtccaa atacacagat
    22501 cgtatcagtt tccggcgatg ggggattttt attctctgcc caggatttag aaacggcggt
    22561 acgtaaaaaa ttgaatatcg ttcatttgat ttggaatgat ggacactaca atatggtaga
    22621 gttccaagaa aaaatgaaat atcagcgggc gtcaggtgta gactttggtc cagttgattt
    22681 tgtgaaatat gcagaagctt ttggcgctaa aggaatccga gctacaagtg tagaggaatt
    22741 agaaaaagct ttagaagaag gatttgcgac agaaggaccc gtaattattg atattccgat
    22801 tgattaccgt gataatgaga aattgggaga aacaatctta ccagatcaat tctattaagg
    22861 aagtgtcgac atgactgaaa aaatcttata ccagcacggg acattaggag ctttgatggc
    22921 cggcttgatg gatggaacag aaacgattgc tcatatatta gaaaaaggaa ctctgggatt
    22981 gggaacattg catgggcttg atggtgaagt catcttttta gatggcgtag cttatcaagg
    23041 aagatcagat ggctcagttg cccaattaga tggaagtgaa cttaccccct atgcagcaat
    23101 tacagatttt acgccagata ctagtttttc tgtctcaaaa actgcagatg gagaacaatt
    23161 gaaaaaagac atattagtaa gagaagccgg ggaaaaccta tttttggcag tgaagataac
    23221 tggattattc aaaaatatgc acatccgtat catgccgaag caagaaaaac cttacagaag
    23281 actagcaaaa atttcagagt ctcaaccaga attccaacaa agcaatgtcc aaggaacatt
    23341 agtcgggttc tttacgcctg aattattcca aggagttgct gcagcgggat ttcacctgca
    23401 ttttatcgat gatacacata catttggcgg acatgtaatg gattttgaag tggctgaagg
    23461 aaaagttgag attagccgca tctcgtccct tgtacagcat tttccagttg atgatccgac
    23521 ttttgttcac gaaacgattg attatgctga tttagcagca gaaatccaac aagcagaata
    23581 gaaaaagaca gcgagatgcg ttcaaggtga ttaacctgac gtatctcgtt tttttgagga
    23641 gaaaacctag cgtttacaaa aaaactgagc tataatcttc tccatagatc aaaaaggagc
    23701 ggtttttatc atgtggacag aaaaacagaa aaaagatgca tacgaaattt tattattaca
    23761 gcaacaagga ttattagaag cagaagataa ctggttagca aatttagcaa attcatctgc
    23821 attgttgaat gagactttgc ctgagactgt ctttgcagga tattatttat tcgatggtaa
    23881 tgaattgatt ttaggaccgt tccaagggcg agtttcttgc acacgtatca agatgggaaa
    23941 aggagtttgt ggagaatcag ctgaaaaaca agaaacattg atcgttgatg atgtcaaaac
    24001 ccatgcaaat tacatttcct gcgattcagc cgcacaatca gaaatcgtag tgccaatggt
    24061 aaaagaaggt aatttaatcg gtgttttaga tatcgattgc ggagtaacga aagggtatga
    24121 tgaaatcgat caagcatttc tagaaaaata tgtagatctt ttattagcaa attttaatat
    24181 aaatgctttt tcatttatta ataactaaaa tcgtctattg aaacaaaaaa gaagagaaat
    24241 caagaataa
//
LOCUS       DANJMI010000040        24056 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000040 DANJMI010000000
VERSION     DANJMI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.040"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(150..1337)
                     /locus_tag="PWU81_001990"
     CDS             complement(150..1337)
                     /locus_tag="PWU81_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HDL1110682.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(1444..1656)
                     /locus_tag="PWU81_001991"
     CDS             complement(1444..1656)
                     /locus_tag="PWU81_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein"
                     /protein_id="HDL1110683.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIN"
     gene            complement(1747..2199)
                     /locus_tag="PWU81_001992"
     CDS             complement(1747..2199)
                     /locus_tag="PWU81_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL1110684.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(2212..2622)
                     /locus_tag="PWU81_001993"
     CDS             complement(2212..2622)
                     /locus_tag="PWU81_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1110685.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            2897..3109
                     /locus_tag="PWU81_001994"
     CDS             2897..3109
                     /locus_tag="PWU81_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1110686.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            3112..3324
                     /locus_tag="PWU81_001995"
     CDS             3112..3324
                     /locus_tag="PWU81_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110687.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            3464..3619
                     /locus_tag="PWU81_001996"
     CDS             3464..3619
                     /locus_tag="PWU81_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110688.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(3616..3840)
                     /locus_tag="PWU81_001997"
     CDS             complement(3616..3840)
                     /locus_tag="PWU81_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL1110689.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            3918..4106
                     /locus_tag="PWU81_001998"
     CDS             3918..4106
                     /locus_tag="PWU81_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110690.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            4432..4767
                     /locus_tag="PWU81_001999"
     CDS             4432..4767
                     /locus_tag="PWU81_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110691.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            5000..5941
                     /locus_tag="PWU81_002000"
     CDS             5000..5941
                     /locus_tag="PWU81_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDL1110692.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            5943..6833
                     /locus_tag="PWU81_002001"
     CDS             5943..6833
                     /locus_tag="PWU81_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDL1110693.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            6876..7676
                     /locus_tag="PWU81_002002"
     CDS             6876..7676
                     /locus_tag="PWU81_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL1110694.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            7691..8542
                     /locus_tag="PWU81_002003"
     CDS             7691..8542
                     /locus_tag="PWU81_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL1110695.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            8743..9204
                     /locus_tag="PWU81_002004"
     CDS             8743..9204
                     /locus_tag="PWU81_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL1110696.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            9230..9682
                     /locus_tag="PWU81_002005"
     CDS             9230..9682
                     /locus_tag="PWU81_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110697.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            9788..9982
                     /locus_tag="PWU81_002006"
     CDS             9788..9982
                     /locus_tag="PWU81_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110698.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            10013..10399
                     /locus_tag="PWU81_002007"
     CDS             10013..10399
                     /locus_tag="PWU81_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110699.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            10396..10599
                     /locus_tag="PWU81_002008"
     CDS             10396..10599
                     /locus_tag="PWU81_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HDL1110700.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            10596..10892
                     /locus_tag="PWU81_002009"
     CDS             10596..10892
                     /locus_tag="PWU81_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110701.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            10968..11384
                     /locus_tag="PWU81_002010"
     CDS             10968..11384
                     /locus_tag="PWU81_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL1110702.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            11645..11782
                     /locus_tag="PWU81_002011"
     CDS             11645..11782
                     /locus_tag="PWU81_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110703.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            12192..12422
                     /locus_tag="PWU81_002012"
     CDS             12192..12422
                     /locus_tag="PWU81_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110704.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            12581..13414
                     /locus_tag="PWU81_002013"
     CDS             12581..13414
                     /locus_tag="PWU81_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HDL1110705.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            13407..14822
                     /locus_tag="PWU81_002014"
     CDS             13407..14822
                     /locus_tag="PWU81_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HDL1110706.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            14834..16363
                     /locus_tag="PWU81_002015"
     CDS             14834..16363
                     /locus_tag="PWU81_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL1110707.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            16281..17333
                     /locus_tag="PWU81_002016"
     CDS             16281..17333
                     /locus_tag="PWU81_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDL1110708.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            17335..17652
                     /locus_tag="PWU81_002017"
     CDS             17335..17652
                     /locus_tag="PWU81_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110709.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            17779..18402
                     /locus_tag="PWU81_002018"
     CDS             17779..18402
                     /locus_tag="PWU81_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HDL1110710.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            18417..19307
                     /locus_tag="PWU81_002019"
     CDS             18417..19307
                     /locus_tag="PWU81_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HDL1110711.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            19330..19545
                     /locus_tag="PWU81_002020"
     CDS             19330..19545
                     /locus_tag="PWU81_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110712.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            19557..19898
                     /locus_tag="PWU81_002021"
     CDS             19557..19898
                     /locus_tag="PWU81_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110713.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            19898..20269
                     /locus_tag="PWU81_002022"
     CDS             19898..20269
                     /locus_tag="PWU81_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110714.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            20262..20657
                     /locus_tag="PWU81_002023"
     CDS             20262..20657
                     /locus_tag="PWU81_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL1110715.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            20659..21036
                     /locus_tag="PWU81_002024"
     CDS             20659..21036
                     /locus_tag="PWU81_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110716.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            21037..21645
                     /locus_tag="PWU81_002025"
     CDS             21037..21645
                     /locus_tag="PWU81_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1110717.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            21645..21986
                     /locus_tag="PWU81_002026"
     CDS             21645..21986
                     /locus_tag="PWU81_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110718.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            22228..24056
                     /locus_tag="PWU81_002027"
                     /pseudo
     CDS             22228..24056
                     /locus_tag="PWU81_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT         8594 a         3608 c         4906 g         6948 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact tttacacaat tatatggacg
     1441 taatcaatta atatcatttt ttaacatagt aatttgttta tttaaatctg atagtgtttc
     1501 ttccttactt aatataaatc tttctctatc atgtaattta tttagttttt catcattttt
     1561 ttgttctaat tcataagctt ctttttttat tgtgttagct ttcgacagtt tagtgattcc
     1621 tataattaat aaaataagtc caatcaataa taacattcaa aactcctcat ttctttgata
     1681 taattattgt agagtttact cagaaatgag gaaagagtcc atgtgaaagc atgggctttt
     1741 ttatttttaa tgttgtaata ctaaatgttc aagattatga ggaagcccta aatattcaat
     1801 aatttggaac tgactcatat cagataaatc ttgttcatct attgttctag ttaacatttc
     1861 aattgcaaac atattagctt cagcttctat accataaatc atacaactaa ggttgtttgc
     1921 tctaaaagct gctgtgcaaa tacctttatg gagtctaacg tgggctattt catgccataa
     1981 tacaaatctt ttcatagaag gtgataggtt ttgattaatt aatatcacat aacatctttt
     2041 attgtgtatc gtttgtccta atgttttatt ccctaaatca gtttcaatta aatctatatc
     2101 agaatatttg attattttat atggattaat cgcatcagat ccaaactcta caaataattt
     2161 ttctatttct tctaatatcc agtatttgtt taaatgcata ccaaagccca cctattgacg
     2221 atatttttta ggagtgaatt ttttctttgc tttaactttg gttaaggcaa gtgcaagttg
     2281 aatggcatcg tataattttt ccttatcttc atcgtctaaa ggttcaccat aataatttgt
     2341 ttcagcatct gaattcaagc cttcaagtaa tttttcagct tgtaaagcaa tatcttgttt
     2401 atctttttct gttaaatcat aataatggcg tttatcagta cgtcctaaaa gataatcagt
     2461 tgatacatta aagtaatctg caactttttg tattttatct ccagatggag tacttttatt
     2521 ccatctagac atgcttccat ttccgaaatt taactttctt tcaagttctg ccaacgtcat
     2581 tgaacgttct tttaataaga tttttatacg gtctaatagc ataggatatg cattcctttc
     2641 aaaataccga caaaaaaagt atttttctgc aaattatcta tttacaaata gagaatttgc
     2701 agttatacta gttttgtaag caagatattt acggcataca aagactaaaa gcaataaact
     2761 aatagtctcc ccagacatca gtaagctcgc tttttgtgcg ctgtattttg tatgcttaca
     2821 taatagcata tttgcagttt ttgtcaaact tattagacaa aaatattaga aaatattcga
     2881 taagggggta tgaagcatga ctttaaaaca gcaagtatta attgctttag taaagaaagg
     2941 ttggagtcaa agagagttag ctcgacgaat gggtatatca attacttatt tacgagatat
     3001 cttcatcgaa aaaagaaaac ctaaggaacg tttaaaacaa atcgaagaac ttttggatat
     3061 caagttagaa gtagattcta atgatcagcc atcagaacag gaggcgtaaa aatggtacaa
     3121 cgtttgagca aagcggagtt taaagagaaa tataagtatt ctgaaagtac ttatcaaaga
     3181 agaatgaagg aatttaaaaa atcacgtttt agtgaggggt ataacgcagt tacttccaaa
     3241 gaaataacaa ttgatgtcga tttatatgaa cagtttagaa catggcgttc taaaaatcga
     3301 tttagagtta gagctgtttg ttaatagcta aaaaatctaa attcaccact tctgaaaaga
     3361 ggagttgctg ctctcctttc aagtttgcca aagtccacgt tttttcattt tggcaactcc
     3421 tcttcttgga ggtggtgacg gcgagagacg aaggagggat gacatgaagg aagaatcaaa
     3481 aatagggatt ttgaatgaga acaattacac aagattaatt attgaaacag aagaagggaa
     3541 aaaagtagca gaagttacat tgaccgatgc aactcctgct gacggttatc gtattcgatt
     3601 aactcctaaa tatgattaac cttttggtgg gtgtggatcg tttccgtgac tatcacggct
     3661 ttgaattttt ccatctctgc catgaataaa tagctcagat ccttgatttt tagaaatctc
     3721 tctagcgatt tttactgctt ctgattttgt attagtatgt gcagtcgctt tagaatttcc
     3781 agcacctttg acattccaac caccattgga attaggtaca acatgttgat tttttcccat
     3841 agttttcacc tccatttcta tagaaatatt ttatcaaata gaaatggaga aattcaataa
     3901 acgaaaggag aagtgatatg gcatatacct tgcaacaaga acatcaaatt ctcggtttga
     3961 ttaaacaacg caggaaacaa ttacaagatg accgtgcagc gcttagaaaa gccgatgagc
     4021 tatcagatag gcaagctgaa ctaattgctt ctgaacttga ggatttgaga atgctagaaa
     4081 taaaaaatag ggagattaga ttatgaagaa gacagacaca ctttttatag gattcatttt
     4141 ggggttatta gtgattgtag cgcaccaaag tattatcggg ggaagcttgt tcgcagcatt
     4201 gatggtttta atcaatctgc ttgattcaaa agaaaggagc aactatggca cgagaagaag
     4261 cgctaaaaat cggtaaagtg attgctgata atcggtgggg aaatagccgc cctattattt
     4321 taagcaagca acatatcgaa aagcaaaaag catggcaaca aataaaaaag tgactctgcc
     4381 agccagcaaa gagtcacata caaatttata aaactaggag aattatatca catgaataag
     4441 aaaattgaaa atcttatcga agaattgaaa cgtgaatgcc aaaagcaagg tgtttctatt
     4501 atttgtacag cccaaaaaga aggggaactt aaaagtctcg tttatggtga gacaacagaa
     4561 attttacttt gtcttgctat gcaagaagaa catttagatg aaaatctccc attgtcagca
     4621 catattatgc gtagaattgc tgtggatgct tacgaacaag cgaaaaatga agaagaaaat
     4681 caaccttcaa atcatacttt tgttattaat aacaaggaag atttagctga tgtgatgact
     4741 cgtattctca agggggaatt tcaatgatga atgaaaaaac aattgatcta ctcattattt
     4801 tatttttcac gttagtgatt gtagcgaaaa gttatgtaat ctttggcttg ttattcgcaa
     4861 ttaccatgct tgtgctttgc gtgttagcaa gcagaaaaga ggttaaacat gaacgagtta
     4921 gcaaatcttg acaactattt aactgatcct gattatgcta aaccaccata tgaagcacca
     4981 attgatgagg aggatgaaga tgagtaaatc taccttagaa atgagccatc aagaatggct
     5041 tgaagaccgt aagaaaggca tcggaggttc ggatgttgga acaattttag gattgaataa
     5101 atggaaatct ccttatcaac tatggctaga aaaaacagga caagtagtac ttgaagaatc
     5161 aggaagtgag cccgcttatt ggggtaatgt tttagaagaa gtggttgcta aagagtttca
     5221 ggaacgtaca ggcaaaaaag tacgcagaag aaaccaagta tttgaacatc cattacatcc
     5281 atttttaaga gcaaatattg atcgggaagt agtgggagaa aatgccattc tggaatgcaa
     5341 aacagctaat caatttctcg gtaaagaatg ggaaggcgaa gaggtaccgc ttagctatct
     5401 ctgtcaagtt caacattaca tgaacgttct aaacaaagac tattgttaca tcgctgtctt
     5461 aatcggtggt caaaaattta tctggaagcg gattgaacga gatcaagagc tgatcgatac
     5521 aatcactgaa caattagtag agttttggga aacaaacgtt cttggaggta tcgagccaat
     5581 tattgatggt agtcaagcga cagctgattt cttgaaagag aagtatgcag atgttgaaga
     5641 cgttcaaacg acgttaccaa ttcatttcga tgaactagtt gaacagaaaa acgaactcaa
     5701 acggactaag aaagaaattg aatcagctat ccgtcaagta gacaacgaga ttatcagcga
     5761 gctaggtaaa cgtgaagcca gtatcggtat cactcaaaaa aacatcatca gttggaaatt
     5821 tgtccgtacg agacgtatga actcgaagaa actagcagag aaatatccag atgtcgcaaa
     5881 tgatgaagag atttataacg ttactgaatc aagaaggcta accgaaaggg agatcaaata
     5941 atatggcaac aaatgaatcg ttaaaaaatc aattggcagc aaagccacag aaacaagttg
     6001 caccaggtca attaggactt aaagctttga tgaatacacc aacaatgaga aagaagttcg
     6061 aagaggtgct tcatgacaat gctaatgctt ttatgtcgaa tgttatgact cttgtatcta
     6121 atgacagtta tcttgcagat agtgaaccga tgtctatcat gagtggtgcg ttaactgctg
     6181 caacattaaa tcttgggcta gataagaatt taggttatgc atatttagtt ccattcaata
     6241 gtaaaaacaa gcaaacagga aaatgggaaa agaaagctca attcatgctt ggctataaag
     6301 gatatatcca attagcccaa cgatcaggta aatacaaagc attaaatgtg atcgaagttt
     6361 acgaaggaga actaaaaagc tggaaccgac tgacagaaga gtttgagttt gatccaaatg
     6421 gtagaacgtc tgatgaagtc attggatatg ttggctattt cgagttactg aatggattca
     6481 aaaaaactgt ctattggacc aaacaagaaa tcgaagctca tcggattgct aacaataaag
     6541 atcgcgataa gacaaagtta agtggtgtgt gggcatctga ttacaatgca atggcacgaa
     6601 aaactgtttt gagaaatctt ctttctaaat ggggaatctt gtccatcgaa atgcaagaag
     6661 ccaccacatc ggatgagaga gtccaaaggg ttcaagaaga cggcagcatt attgctgaaa
     6721 cagaagttga agaagatatt cctgaaagaa aagaagcaga ggttatatct gaagaaaatg
     6781 aagatgtaca aactggatta tttgatgcat ctaatccgcc gttaaacaaa taatgaggga
     6841 gctttctccc ttgcttttct agaggagaaa tacgaatgaa cacaggatat gtcaaattac
     6901 atcgaaaagt gatgaattca ttcgtttgga ccaatcctta tatgtataaa ttatggaact
     6961 tgtgtttgat gaaagcgagt catgagaatc gcaagatact ttttaatgga aaagagatac
     7021 aagtgaacag cggagaattc gtcacagggc gcgatgctat cacatctgag atgaacgaag
     7081 gcgtgaaacg tgaacatcaa gtgaacagcg gttctgtatg gagatggcta aaacaatttg
     7141 aaaaaaacgg aatgttgaac atcaaatcaa ctacgaaata cagcgttata tcaataaaaa
     7201 actggtcttt gtaccaaagc agtgaacaac aaatgaacat caaacgcaca acaagtgaac
     7261 aacaagtgca cacaaacaag aatgaaaaga attataagaa tgaaaagaat aataaaacat
     7321 catcgtcgca accacgcaaa aagcgtgttt acgacaccga ctcagtttac tacattctcg
     7381 cggaggagtt attcaaacag atttgtcaga atcaggaaat caaaaagccg aatctgcaaa
     7441 gttgggcaga taacattcgg aaaatgatcg agatcgacaa gcggaccgaa aaccaggtac
     7501 ggggaatgat tgaatggagt cagcacaatg tgttttgggc atcgaatatt ttatctgcta
     7561 aaaaattgcg agaaaaatac gacacaatgg cagcgcaagc caatcgtgat tataaaacaa
     7621 aacaaactaa aacgcttgaa tacgagaaat ttagcacaga tgagttgcct atttgagagg
     7681 aggcgtaggc atggaaactg ttggggaaat catggaaaag ctgatacaga aagtgcttgt
     7741 ccaacgtggc gaatgtcctg aatgcggaca gcctttgtat ggatggcgta cgaagaaccc
     7801 tgatggctca gaacgttgta agccaacctg tatgcaatgt gggtataaag cattgcgagt
     7861 tcaggaagac ttacaaacag aacgaattta caacgagagc ctgaaagcaa gagcaatcaa
     7921 ttttttcaaa ggtggttctg ttgtgcctaa tcaagcgttg tttgattgca cattgcagaa
     7981 ttatcaaatt gtcgatcaag aaacaagaca agcggttgaa gtaaccaaac gctttgttaa
     8041 ttcagtcttg ttaggaaatc caagtcacct tgttttaact ggaaaacaag gaacaggtaa
     8101 aagtcaccta gcgatggcag cggcttggga agtcttgaag cgatcaaact acgataaaaa
     8161 aatcttattt atcgggttac aggaaatgct ggatcaaatc aaattttcgt acaacaatcc
     8221 tgaactcaga aaaacgattg agggatcgtt gattgcagat atcaaaacag cggatttagt
     8281 catcattgat gatatcggtt cagaactagg aaaagatgca tcagatagtc gagcgtttgg
     8341 cataaacacg ctaaattcgt tcttggatgc acgacagaac ctagcaacga ttatcacaac
     8401 gaacttgctt ggtgaagaac tgaaaaaagc ttacggtacg agaacaatat caagaatgtt
     8461 tgtcaactct gatggattta cgatggtatt ttctcaaaca gcagacaagc gcataaaacc
     8521 agtcaaagga gcgatcgcat gaatttgact aaacaagaaa aaagaatctg tcgtcagata
     8581 atgttgcaaa ttataggtga tcgtgacatg gacgaattat ctcaacaaga gagtcgaatg
     8641 gttcggaaga taagtaagct aattatttat cgggatacag aaagatttct cactgtttta
     8701 ggtaacgtct acgagaatcc agagttattg gaggtagagt aattgccaaa aatattagat
     8761 gcatgttgcg gtagtagatt gttctggttt gataagaaca attctgacgt aacatttatg
     8821 gattgtcgtc agcagtatga agaattatca acaggtcatg tgatcaatgt tgatccagat
     8881 gtggtcgcag actttcgcga tatgcctttt gaagatgatg tgttcgatat ggttgtattt
     8941 gacccgccac acttaagaca tgtcggcgaa aactcatggc ttgccaaaaa atatggaaaa
     9001 ttagacgaac tttggccaga agacatcagg caaggttttg cagagtgtat gcgagtttta
     9061 cgaccttcag gaacattaat ttttaagtgg aacgaggaac agataccact ttctgatgtg
     9121 ttagaggcta ttggagaaca gccattgttt ggtaataaac gaagcaaaac acattggctt
     9181 gtgtttatga aaaaattcag ctaacggagg aaacaaagga ggaaaagtaa tgaagattgt
     9241 attaaataaa tgttttggtg gctttgggtt gagccatgaa gcaaaaatgg aaatttttaa
     9301 aagaaaaaat attgaagtat tcccatacat caataacttt agctatgact cagatgatga
     9361 atacacgaga tacacaggcc aaaagctagg ttcgatggat tttatctact atttcaaaaa
     9421 agacccgaaa atcgacaaag ttacaggaat ttatagcgaa attgaccgac tgtacggaat
     9481 tgctgatgat tccagttttt caagtgattc aaacagaggt gataaggacc tcgttgctgt
     9541 tgtcgaaaaa ctaggaagtg aggccagtgg accatatgca agtcttaaag tcgttgatat
     9601 acccgatggg gctgagtggg aaataagtga ttatgacggc gttgaaactg cacattatgg
     9661 ttttcaaaca ggtagtattt agtccactaa tgacaactaa agaggaggca gaaggatgag
     9721 tgaagatgaa tactttgaac agtagtctat taacgacaga gatttttaac tggaaagggg
     9781 tgtagtcatg gacgcatcgg aagtagtaaa taaaattaat agtctcaaag aggttttcgg
     9841 agacgtcgaa gtagtgataa acgcacaagg ctatgacaac ggatttttta agcagattac
     9901 cgacattagc attcagcaag gaatggaaga cgaagatgga aactttatcg atgaagtagc
     9961 cattttagtg acttgcgagt aagtaattga cagctaaaga ggaggcagaa ggatgaaaaa
    10021 attgcaccaa ctcatatctg agaaagaaag cgaactccaa aatttagaag atagtttggg
    10081 attggggttt ccaattgttg agcaagctaa aatgactcaa atcagtcatc tacgacttga
    10141 attggaagat ttaagacaaa tagaaaaatc aattcaactc aacgacaacc agaaaatcgt
    10201 gttggagtgg ttgaaaagcg agacaatttt aactagagaa gcaccaatat tatctgttaa
    10261 tgctttttct gataaaaatt tactaggaaa attacctgat aaagtacgca aagcttataa
    10321 actattggct tgtaaacaag aatatgaagt cctatcagca ttcgctcaat ggggattaga
    10381 acaggaggaa gcggaatgaa caacagacat cgcagaataa caaaactaag aaaacaggaa
    10441 ctgaatgtac taaagacaaa gtttgaaaaa gaatatggaa tttcagcaga agaaacatat
    10501 aaagtggcaa gtcagtttgt tgctgatgcg agtaatgcta ttcgtaagtt tgggatttcg
    10561 atattaaatg atgatcgtaa atgggaggaa atgagatgaa aataaaagac ggattttacg
    10621 ctagtagtca tggtatcggc ggtttaatgc tagacatgcc gataaagaac cctaaaacac
    10681 gtcagaaatc aaaattcaaa atcggtgaca tggtccgatg taaagcagaa gagttcatct
    10741 atccgtttcg tggatatgta gagcatgtct ataatcattc agcaatcatt cacattgaaa
    10801 acacgatgga atgcgataag tggttagcga aaagcaaaga taatttagca gtggctcgtt
    10861 tggtggatat ggaactaatc aatgacaaat aaaaaagccg gatcgctccg actgattcaa
    10921 taaatccaac acatttatta tatcacataa aggagcggtt tgacttgatg caattgttac
    10981 gagaggtaga tttcaaacag acaagatgta atgcgagaga tgtgctgaag aactttcggc
    11041 gtttggagcg gatggcaggt cgctctttga tagatattaa gtcgccgatt attacggata
    11101 tgccgaaggc accgaagcac ggcaataagg cagaagacgc gatcattcag atgatggata
    11161 tagaagcgga gagagacgca atactagcgg ctttgatggc tcttagtctg attagccgtc
    11221 agatactcta ctacagcttc tgtgacgtaa acaagcactc taattatgaa atagggcaat
    11281 tgatacgagg atacggagag aagaatgtag agaagttgaa atccatcgca ttaatcgaat
    11341 ttgcagaagc atacaaaaaa ggcgtgttag ttcagtatcg ttgattttgt agggtttttg
    11401 tagggatagt gtagggtttt tgagcggttt aacgtgatat tatggtagtg tcgaaagatt
    11461 agtgataggt ctgagacaaa ataaaatgta agggaggaaa tctccctcat cgttttaact
    11521 taagcttcga tagacagcaa cggaaatatt aagaataagg atgtgaattt caactccttc
    11581 taaattgttc ttattatcta tcatccgttg ttgtctatta atttatgcat cggaggaaaa
    11641 acgaatggat aaagaaatca aagtaactgt caaattagat ttgactgaac taaaagaact
    11701 gctcaacaag gctagtgacc aagtcgaaca gttacaagaa actttagatg aaattgctaa
    11761 ttttaaaatc caagtttctt agcagtatat tttttagcag ctgcactcat aaaatcatac
    11821 caagtatcag aaaaatataa cttaactaaa taattaaaaa aggtgggtaa atagaaatga
    11881 cggcgttaat aatttcaatt tttgcgctct gtcttaatgt ctatatgatt ggatttaaaa
    11941 atggacaaaa taaaaaatag tagcagccga gaataatttt atagtgtcac tgtggcggaa
    12001 atagaagacg caaaggtgga ggtaggaagc gtatggcgca ctatcgggac cttgtacaaa
    12061 actagaaagg actagtacgt gtgtggtgcg agccctaggg aggaacagga taaccgcctg
    12121 tgtgtaggtt gctattacat attagatcac tctttgagtg gtctttttat ttacataaag
    12181 gaggctacat aatgagaaac tactggtata tatcgctaac taatgaatat cctcgaacca
    12241 ttgatgattg ttcagtgcgt gttgtgcgtt ctgtccaaat caaaaagaag tactccattg
    12301 ttgaaatgac cagagaagct acgccaaaag agattgataa atgcaaactt ctttattgcg
    12361 gtcatggttt ctatgatgaa ccaaacattc aaaataatat taacaagaat ttgagggatt
    12421 agaatgcaag aaatggctta aaaatctcca atcaggacaa atcaagttag acaaagtttc
    12481 cgatttaaag atattgattg aagctgattt aatgctaaaa gatattgaaa attagaaaac
    12541 aaaactcaac ctaagaagat tgcgaggtgg tgtgtattga atggcaagac aacgtgatcc
    12601 aagacgtgat gaagccaaaa gaatttggtt agaatccaac ggagaaaagc agttaaagga
    12661 aattgcatct gaattaaatg tttcagattc tcaggttaga aaatggaaat cgcaagacaa
    12721 atggagcgct gaattgaaaa gtaacgttac caatggcaaa agtaacgtta ctaatcaagg
    12781 tggcgctcct attggtaatc aaaatgctaa aggtaacaaa ggaaacagca gagcatcacc
    12841 gccaaagaga aataagaatg ctcttaaaac tggtgagtat gaaacaatat tttttgacac
    12901 gttaaccgat aatgagaagg acatctattc tagtttgaat gatgatcctt cttttgcttt
    12961 gtccgaagaa atacgacttc ttaaaataag acaacttcga atgatgaaaa gaatccaaca
    13021 agctgaagct ggactaaatg atgaagaagt cgaacgattg cagcagctaa gaaagattaa
    13081 aactccgatt gaaaaaaatg gtaaaaagct agaaatcaag cgtgaggtta tgcaagatgt
    13141 gcagattagc agaaaaaaac atcgcaaaat tgatgatatt ctttcaattg aagattcatt
    13201 gactcggatt agcaaccagt tagctaaagc catcaagcaa atgaatgaac tttatatgaa
    13261 tgaatacaga actgatttaa ttaaagctca gactgataag atccaagctg agacaaatga
    13321 aattggcgga aataattcag gtgaagaaat agaagaatgg aaacaggcag ttttaaatgc
    13381 cgcaaacaaa cgggcggtga aagaaaatga ataatgaatt tattcctttt gctgatattg
    13441 gttctgccat tgattattac tatgataaac cagtagcttt ttgccaagat attttgcatt
    13501 tgaatcctga tgaatggcaa gaaaatgttt taaatgattt agctgaattt tcaaaggttt
    13561 ctgttcgttc tggtcaagga gttggaaaaa cagcattaga agcaggagca atactttggt
    13621 tcttaacgtg tcgaccctac gctaaagtaa tagcaacagc tccgacaatg aagcaacttt
    13681 acgatgtact ttgggcagag gtagctaaat ggttaaatga tagcttgatc aaaaacttac
    13741 tgaagtggac aaagaccaaa atttatatgg ttggtgattc agagcgttgg tttgctacgg
    13801 ctagaacagc gactaaacca gaaaatatgc aaggttttca cgaggaccat atgttgattg
    13861 tggtagatga agcttctggt gtgtctgatc caattatgga agctattctt ggtacgctat
    13921 caggttttga taataagctg ttgatgtgtg gaaaccctaa taatattgaa ggtgtctttt
    13981 acgattccca caattcagac cgtgataaat acagagttca taaagtatca agctatgata
    14041 gtaaacgtac aaacaaagac aatatagaaa tgattcttaa aaaatatgga aaagaaagtg
    14101 atgttgctcg tgtccgtatt tttggagaat ttcccaaagg tgcgttggat tcatttatca
    14161 gtcttgaaac ggttgaattg gctacagaaa aacaaattag tgattcttta gtcaataaaa
    14221 caacggttgc tcatattggt gttgacgtag ctcgatatgg tgatgattct acgattctct
    14281 ttcctagaat tgctaccagg gcattggagt atgagaagta ttcaaaacgt agcactatgg
    14341 aaacaacagg atatgtcatc aacatggcca agaatctaat gagtcaatat ccgagtattg
    14401 ataaagtgat gattaaagtc gatgacactg gtgtcggagg tggtgtaacc gaccgcctag
    14461 aagaacttat agaagacaag cattatcctt ttgaggtgtt tggagtgaat aacggttcaa
    14521 catcagaaga cgatttttac gataatttag gtactcaact atggggaaac atcaaggaaa
    14581 tgttagaaga aaatatgaca gcaaatctta acggagaaca gcctgttatt gaattgcctt
    14641 ctgatagttc gttaatcaaa gaattaagta ctcgcaaatt caaaatgaca agtagaagtc
    14701 gtatacgttt agaaagtaaa gatgatatga aaaagcgaaa tattggtagt cccgatattg
    14761 ctgacgcact ggctttagcg ttttatgagc caccaagtca ctatcaattt attcaatttt
    14821 aggaggtgag tttttgatta gtacagtatt atctttagag agatataaga agctacgtgt
    14881 taaatatgca acacaaattg aagatggaat gtttgatccg aatggtttta tagaatatat
    14941 gaaaccattt tttgctgatc gcgaaagaaa atatctagct tatactagcg aaaagaatga
    15001 aatagacaat agaccaaagc ctaacactga tattgtaaaa gttaataata aacttcatgc
    15061 tggtatgtat tcaattgtcg tagaccaagc agtcaatcat tttactggta tacctatcaa
    15121 atgggattac gatgtatcgg aacaaaaaag aacactcatt caaagattaa aagataaatt
    15181 cttaaaaaat gatatagaac ttccaacagt tccagaagct tttaacaaat taacaagcaa
    15241 tcttgattcc atgagattcg caatgcttga ttctgaaact gcaacttttc aaggagcttg
    15301 tggagtagct ttccgattgt tagagcctgt ggaagaagat gatggttgga agttaagagc
    15361 aagtaatatt gaaccttgga gagcagaaag atatggaaat gccggaatct atatcaaaga
    15421 aaagtatgac tcttaccaga aaaaattttt tcaagaaatg aaagttatga caagaaataa
    15481 aattctgaca tatgcttgct atggtgattt gaattttgtt acaagcggaa cgttcaaaaa
    15541 aattgatgag accgataacc ctttggggac gattaccttg gcagaattta aaaataatac
    15601 gaatcgttat tgtgattttg aagtagctga ggaaattgga gatgcaattg atcgtgcttt
    15661 gtctgatcaa caaaacgaaa tcgaacaatt taagcttgcg tacatgcttg ttactggcac
    15721 aacaatgagc aaaggcactg ctaaagaaat gatgaatcag ctagggatta tcaacttaaa
    15781 agaccctact gcaaaagctg aatatgtcac aaaaaaatta gcaaaagatt tcaatgaata
    15841 tcatatggat ctattgaaaa aacagtttta caccatttgt aaagcaatcg attttaacga
    15901 tgaggtgttt aaatcaaaca gttctggaga agctcgcaag tggcaaatca ttagcctaga
    15961 agcgaaaaca aatactaaag agcaatattt tagagaagga ttgaaagaat gcgcagaaac
    16021 aattgctgct tttcttaagt ttcatgacaa agtagaaatt gagcctgaaa aaattatttt
    16081 tactttctct agatctttgc caactgattt aagttattta gctgaagctt tacctaaact
    16141 tgcaccatac gtatccaaac gaactattca gagccaaatt ccatttgtaa cagatgttga
    16201 ttatgaaaat gaaatgatgg aattggaaag tggaagtgct tatccagata gtgaatacaa
    16261 ttttggcgga ggtggcaata gtgacgatag aaacgaagta ttggaccaaa cgtcgagaac
    16321 tggaggatca agcaaggctc aaacaagaaa atcagacact taaaaaatta actagtgtat
    16381 ttcctgaagc actgaaagag atacaagcaa aactattatc acaagctgac ttacacaata
    16441 tcacttatcc agaaatgatg gagttttaca gtacaagtaa tcagaaaaaa tatcgtgaat
    16501 atgtggaaaa aaattataag tcattaaaaa tgtatgatgc aaaatacaaa gagtttatcg
    16561 atgaattttt tccaccattt gactatgcaa aagtcaatcg cttattacaa atacgatcag
    16621 atgtatttaa aattcttgca gaatatgcga tggatgcaga tgtgaatcaa tatttttctg
    16681 atcgcttaga ggaaattctt caaagaacat attcttctaa tgctaatgtt tttgttcaac
    16741 ttttaaacgt tgatatacca aattatttac cagaaaatga aattaaacat tatttgaact
    16801 atccatggtc aggaaagaca ttttcaagaa gactttgggg aaatatttca tcgcttgaac
    16861 agaaactctc taatgctatt gtaaaaagtg ttgctagtgg agaaggcgtt atacacgcat
    16921 taaacaccat gagattagac tcagaaattt gtgacatgtt taagttagaa gaatcaaagt
    16981 ataacaaagc gatagaaaat ctcgttcgaa cggagtatgc aaaatttgca caagatggta
    17041 ttgaaaaatc atatttagaa acaggtattg aggaatacaa cgtattgact gcaaaagatg
    17101 agagagtttg ccggatttgt ggaggaaagg caagtaagaa tccctataaa ctgaaagatg
    17161 ctgtcatagg tgaaaatcga gcacctttcc atagtcgttg tagatgtacg gatgttccta
    17221 atttaccaaa attaggaaag gatattgatg aagaatatga ccgtttattt ggcgatttat
    17281 tagatgagtt tgcacatgat tcttttggaa ttaatttgaa acggaggaag tagaatgaaa
    17341 gatttttttg aagcagtact aacaattaat gtaaatgctg atattgcaga agcctacaaa
    17401 acagctattg agtctgagaa ccatcctaat ggcttgagag accattggaa tggcaattat
    17461 gcctacgtgg ttattggcga tcaaactgtt aattatcaag ataatactcc agttgataag
    17521 aataccgtta atttaacgat tcaattatta tctcattcat taccaaattt aaaagaaaca
    17581 gttgattggt atgaaaagat gggatgtatt gttgttagaa ctgactacaa agaaggaaag
    17641 tctagtaatt aggctttttt attttgtccg aaatgacgtt aaactagcgc aatactgggc
    17701 ttggttgaat ggtggggcgc aactattaaa actcaaagca atgcggggcg tgaaaacgaa
    17761 tcgtggggcg aaaggagaat gagcatgaaa cacaaatcat taatgccaat gaatttgcaa
    17821 tactttgccg aaggtgacga tcaaaagttt tcttttgatg acttcaaatc ttttgtggaa
    17881 tccaatgaag aagctcaaaa atttattcag tcacaatctc aaagtgttgc cgacaaacaa
    17941 ttggaagctt ggaaacaaaa taacttagat aaaattaaac aggataccat caaggaatat
    18001 gaggaatcta agaaaaacaa gtcacctgag caaattcaac tggaaaaact acaagctgaa
    18061 tttgaagcag aaaaagcgtt gcgtgtgaca agtgataata aagcatttgt tgcagaacaa
    18121 attgctggat tagaactaga tggagagtta aaagagtcta tttctcaatt tatgctaaat
    18181 aatcttgtta gttcggatac agatttcact aagaatgctg ttgaaggttt tacaagtgtt
    18241 ttgaatgcaa ttaatgagaa acatgcagat gcattaaaag aactacaaat gaagtctgca
    18301 tttggtggaa ctcaacaatc gaataaccag gttcagcaga acaatgaaac attaacaaat
    18361 ccagaagaac aattaggaca aattcttcaa caatttaact aggagagtga aaaattatga
    18421 aaaaaacatc tttaaataat ttagagtatt tggatatttc accagcgatt aatgctatgc
    18481 aagtaccaaa tacacctttt ttaagctatt tacttggtgc tggaaaaaca gagccagcaa
    18541 actcgacaga aattaaatgg cgtgaatatg atatcaacaa cgatgattct tctgaaaaac
    18601 ttgagggcgg agaatatcca gatgctgaat caggtcgaac ttggtttaac aactatactg
    18661 aaatttttag aaaatcaacc tctgtatctg gtacattaga tgctattaat gtgaatggag
    18721 tcggaaatga attaactaat caagtagcac tacgtggtat ggaaatgaaa attgatttga
    18781 accgaaaatt gattactggt gtaaaagctg atgaaaatgg ttctaaaggt cgtcgaatga
    18841 atgggatttt gaacttgatc aattcagcaa ataaggcaga aacagccact gcgggtgcag
    18901 taacaagaaa agatatcgat actttattta aattgatgta tgaaaaaggt tatatgggag
    18961 aaaaactatg cttgatttct ccagatatgc aggagttaat gactgatgag ttagatggaa
    19021 aatcaacaaa aattgttcag ttcggggaaa gagtaacttt tggattgcaa attggaaata
    19081 tcgtgtctaa ttacggtaca ggtattgctc tactagaacc atcattgcca aaaggaacaa
    19141 ttgccgcaat cgatactaat tatgtgaaac tacgtccatt acgtgaatgg agagcagaag
    19201 aacttgcaaa aacaacggat tctagacgta tcggtcttgt aggtgaatac tctcttgaat
    19261 acaatgcttc aaactctggg gcaattttaa atttaaagtc tgaataaaag ggagttagta
    19321 ctcccttttt tgataggagg aattacagtg gtaaaaaaag atgaaactaa aaaagatgaa
    19381 gtcgtgaaat atagagtagg taaaactaaa aattttgttg ggtttgttca tcctaaaact
    19441 cgtagattta tcacagcaga ttcaaataac gaatttatca tttctataga tgataaagaa
    19501 gcaattgcaa ttttggaaga tgcaattgat gttaatgaaa tttaggaagt gatctgatgg
    19561 atgaatcgct aaaaacggaa atcattgagt ctacaaaaga agattttcca gatttgagtg
    19621 aagaacgcat aactaattta ttagaaataa ttttgctaga aattgaatca tacaacactt
    19681 gtaaaaatga tatttcatgg gaaaagttaa aaagcgtgat tagtgaagtg ttgtataaaa
    19741 taataaaaaa tgaatcagaa aaaacagtat cttcagttag acgtggcgat acaacgatta
    19801 gttatgcttc aacaacaaat gatgttagtg agttacttct aggctatggt gatttgatac
    19861 gaagagttat tggttgtgga ggattggagt ttttttaatg aatgaagcag atattttaga
    19921 aactacttat gaagatagtt gtattattga aagactgacg gacattgaag attctaatac
    19981 aaatattact attcaagatt acaaaaaagt atatgataat cctatttctt gcgctctttc
    20041 acaaggtcaa atcgatggac tagcagtcat agaagatgga gagatggtaa atgtttcaac
    20101 tgacacatat aaattatttg ttcatcctaa gattaaacta aaaaaaggag atcgaataac
    20161 aataactcaa aaggccagtg gcttaatttt ttctctattt gctactaagc ctttttacta
    20221 tcctagtcat tgtgaagtga atttgatagg aagtgagaaa aatgggtaat ctcaaatatg
    20281 aatcaaatgc agaaaagatc attgagaatt ttaaaaatat gactgtaatt gctcaaaaag
    20341 aaggaatatc cttcgttaac gattcaatga ataaagtcgt tagtttagct aaaccattaa
    20401 ctcctgtaaa atcaggtaat ttaaggcgag gatatcgtgt agtaaaagct agaaaactat
    20461 caagcggtcg aattgttgga gcagttataa acaatgaaca ttattttaaa tatgtagaag
    20521 aaggtcatag gactaaaaat ggtggctttg tcaaagggaa attcatgtta actcgtgcaa
    20581 cgaatcttgc aaatatgtct tatattcctc gaagatttaa acaaatggca ataaaaatcg
    20641 ttaagaaagg aaagtaacat gaaagatgaa atcattgctg caatcagtag caaattaaaa
    20701 gaaatctatc ctgatgggac aatatatctt gattcagtta tgcagtcaac taaagatttt
    20761 tatttcgttt tatcagtaat ggaatctgga actgaaaatg taggaattga tattcaaaat
    20821 gtttctttct taattgatat tgcattgatt gataataaac ctaatagaaa tttaattaat
    20881 gaattagtct cacgttgtgg gacttttttt aatacgatta caatcgatga gcaaatatta
    20941 tttccaaaag cctatttacc tgatgaagca gatggtgttc aacatattcg ttttacatta
    21001 gaatttccac aatatattga atggagtgaa agataaatgg gagaaaaaag aagtaaaacc
    21061 ggaattattt ctgtcgaaaa acctacttgg tttcctttgg aagatgagac agggaatttt
    21121 ccggtatatg gtactgcgtt gccaatggga acagcagtaa gtatcaaacc aacagctaat
    21181 tatgaaacaa cacaagatta tggggattca gtggttcaag atcaatttac agcttttggt
    21241 ggtgcagaag ttgaactgga agcaaatgga tatactcata aagttttatc tacaattact
    21301 ggagggaaaa ttgttaaagg tggtgccttg cgttctggag aagatattgc gcaagatggt
    21361 gcgtttgcat atagacgtaa aaaatcgaat gggaaatacc gttatacggt cttttataaa
    21421 ggacagtttg ctctagattc agatgaaaca tcaacgattg aaggaagtaa agtaagtttt
    21481 actcatccaa cttggaaggg ttcatttgta gatgtaccag gacttggata tatgtattca
    21541 gttgatgaag atgatgaagg cgtggataaa gcaatgatcg gaaattggtt tacaaaagta
    21601 gcaattccaa ttgaagaagc agaactttca ggaggtacag aataatgtca aaatatcaaa
    21661 caacgattaa attaacgaaa aaagatgaag aaggtaaata tgagcaagta caatttaaat
    21721 ctgctgaatt tctaccaggc ccagtcgtag aagaagcagc agaagtaatg gagggaatgc
    21781 aaaatgctgt agatggaaaa tcagtatcag aagcattaag tcgagcttat tcatttattg
    21841 cagatatttt gtttgaagga cagtttactg gtgaagatta tcgtcaaggg atcgatgctc
    21901 gagaaattgc ttcgttgaca ggtaagttat taaaatctgt tactgcaggc tttgatgaaa
    21961 cttatacaga aacgaaaaaa aagtaagcga agctctcaaa tatccttttt ttaaacattc
    22021 gattacttat cgagaattag atttaaagac gcaattactc gaagcaggtt ggacgttacc
    22081 agaaattgaa cgtactgact ttgacgagtt aatgcgtctt tttgcttttc gagatgcagt
    22141 aaaagagcat gaggatgttg agtactacga taacttcacc caattttagg aggtgatact
    22201 ttgaataacg atgacctaat tctgaagatg atactagatg aatctggttt tactgccggt
    22261 atgaataatg ctgtcaaaaa gttgaactct tttgatgaaa caatcgaaag aaaaagtaga
    22321 aatagtggca gttcattagg tagtatttgg aaaatatttg ctggaagttt tctagctagc
    22381 ggagtaacta gaatcgtagg agctggcttt gatctaatca agggttccat aagtggagcg
    22441 gttgatcgag tagatacgat gaataacgct ctacgaaatt tccaaaatat gggatttagt
    22501 aattcagaaa ttatgaagaa tatcggaaag aatgggcttt tatctcaagg tattcaagga
    22561 cttcctactg ctttgaatga tgcgataagt catgttcaac ttcttgcttc ttctacaggt
    22621 gatttaactc gttcaactca aatatttaaa gctttaaatg atggaattct tggtttcggt
    22681 ggttcaactg atcaagttaa tgaagctgtt attcaattat ctcaaagttt ctcaaacgga
    22741 aaagtagacg cacaaacttg gaactcaatg atcaacgctc aacttggacc tactctttct
    22801 gctattgcta aaaagatggg aattacaatg ggagatctga aagaaggttt atctcaaggt
    22861 aagatttctg ttgaagaatt ccaaaatcaa ttaatagaaa tggataccaa aggtggcgga
    22921 ggacttaaat cattaagtca aatcgctaaa gattctacta agggaattaa aacctctata
    22981 caaaatgcaa aaacagctgt tacacgtggt gttggtgagg taatagaagg attaaataaa
    23041 gcattagtag attcagattt aggtggattt aagggaatta ttgataaagt gggcagttca
    23101 atggaatcgt tcttaaaagt aattgctgca aatattccta cagcagtatc atttttaagt
    23161 aatttattta acgaaattca aaaatttggt tctgctttga aattcatgat gccctttctt
    23221 gttcctgcag ctaccgcttt tggagcgttc atgtttcaac ttaaaggtat accagcaata
    23281 ataaaaagct tcaataattt taagaatgcc ataattggcg ttggaaattc attaaagatt
    23341 atgggggcaa tagctgctgc taattcattt gttttaatta ttggtgcagt cgttggtgca
    23401 atagctgttt tcggttactt tatggcaacc aatgaagagc ttagaaacaa ggttatatct
    23461 atttggaatg atgtgaaaga ttccgtaatt ggtgtattaa aaaatataaa ggactgggga
    23521 attgatactt ggaattctgc taaataaatg gcatcaaatg cagttgaggg tgtcaaagat
    23581 gcttggtcag gaacaaagga atggttttct aacacctgga aaggtattaa ggatggggcc
    23641 acaggtttat ttgataaaac agtagagact tcaagaaatg cagtcgatag tgtaaaaaat
    23701 gcatggtcta atatgaagaa atggttttct gatacttggc aaagtataaa agattcagcc
    23761 agagaaaaat ggaatggaat caaaggttct attatgtcag ttgctggtcc attgattaca
    23821 ggtataaaga atgcattttt acatgttact ttttatctag agaccttatg gaataattta
    23881 gttgagattg gtaaaaacgt ctttgagatt ttaaaaaatg ttatacttgc gccggttttg
    23941 tttattacct ccatgatttc tggtggatgg gaagaaacaa aaaataatat gattgctgtt
    24001 tggaataata ttaaagaaag tgccataaat atttgggaat ctataaagaa tatatt
//
LOCUS       DANJMI010000041        23827 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000041 DANJMI010000000
VERSION     DANJMI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.041"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(53..787)
                     /locus_tag="PWU81_002028"
     CDS             complement(53..787)
                     /locus_tag="PWU81_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL1110719.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="PWU81_002029"
     CDS             1114..1524
                     /locus_tag="PWU81_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL1110720.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="PWU81_002030"
     CDS             1517..2218
                     /locus_tag="PWU81_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL1110721.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="PWU81_002031"
     CDS             2218..3804
                     /locus_tag="PWU81_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1110722.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="PWU81_002032"
     CDS             3823..4044
                     /locus_tag="PWU81_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDL1110723.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="PWU81_002033"
     CDS             4041..4802
                     /locus_tag="PWU81_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HDL1110724.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="PWU81_002034"
     CDS             5182..5691
                     /locus_tag="PWU81_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL1110725.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="PWU81_002035"
     CDS             complement(5766..6305)
                     /locus_tag="PWU81_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL1110726.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="PWU81_002036"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="PWU81_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL1110727.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="PWU81_002037"
     CDS             7318..8169
                     /locus_tag="PWU81_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDL1110728.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="PWU81_002038"
     CDS             8197..8832
                     /locus_tag="PWU81_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDL1110729.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="PWU81_002039"
     CDS             8852..9625
                     /locus_tag="PWU81_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1110730.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="PWU81_002040"
     CDS             10033..10830
                     /locus_tag="PWU81_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDL1110731.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="PWU81_002041"
     CDS             10808..11221
                     /locus_tag="PWU81_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL1110732.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="PWU81_002042"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="PWU81_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL1110733.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="PWU81_002043"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="PWU81_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL1110734.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /gene="maf"
                     /locus_tag="PWU81_002044"
     CDS             15998..16558
                     /gene="maf"
                     /locus_tag="PWU81_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDL1110735.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="PWU81_002045"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="PWU81_002045"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDL1110736.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="PWU81_002046"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="PWU81_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDL1110737.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="PWU81_002047"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="PWU81_002047"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL1110738.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="PWU81_002048"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="PWU81_002048"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL1110739.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="PWU81_002049"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="PWU81_002049"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL1110740.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="PWU81_002050"
     CDS             21905..22483
                     /locus_tag="PWU81_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1110741.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="PWU81_002051"
     CDS             22535..23017
                     /locus_tag="PWU81_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDL1110742.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="PWU81_002052"
     CDS             23134..23508
                     /locus_tag="PWU81_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110743.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7780 a         4286 c         5115 g         6646 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggtat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagtt
//
LOCUS       DANJMI010000042        22685 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000042 DANJMI010000000
VERSION     DANJMI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.042"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            66..3344
                     /locus_tag="PWU81_002053"
     CDS             66..3344
                     /locus_tag="PWU81_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HDL1110744.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3885..5792)
                     /locus_tag="PWU81_002054"
     CDS             complement(3885..5792)
                     /locus_tag="PWU81_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HDL1110745.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5922..6797
                     /locus_tag="PWU81_002055"
     CDS             5922..6797
                     /locus_tag="PWU81_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HDL1110746.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6877..7851
                     /locus_tag="PWU81_002056"
     CDS             6877..7851
                     /locus_tag="PWU81_002056"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL1110747.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8031..9176
                     /locus_tag="PWU81_002057"
     CDS             8031..9176
                     /locus_tag="PWU81_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL1110748.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9313..9657
                     /locus_tag="PWU81_002058"
     CDS             9313..9657
                     /locus_tag="PWU81_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HDL1110749.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9928..11214
                     /gene="efmA"
                     /locus_tag="PWU81_002059"
     CDS             9928..11214
                     /gene="efmA"
                     /locus_tag="PWU81_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HDL1110750.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11352..11951)
                     /locus_tag="PWU81_002060"
     CDS             complement(11352..11951)
                     /locus_tag="PWU81_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HDL1110751.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12355..13476
                     /gene="mnmA"
                     /locus_tag="PWU81_002061"
     CDS             12355..13476
                     /gene="mnmA"
                     /locus_tag="PWU81_002061"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL1110752.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13722..15143
                     /gene="arcD"
                     /locus_tag="PWU81_002062"
     CDS             13722..15143
                     /gene="arcD"
                     /locus_tag="PWU81_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDL1110753.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15157..16275
                     /locus_tag="PWU81_002063"
     CDS             15157..16275
                     /locus_tag="PWU81_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDL1110754.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16335..17627)
                     /locus_tag="PWU81_002064"
     CDS             complement(16335..17627)
                     /locus_tag="PWU81_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL1110755.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17759..18337
                     /locus_tag="PWU81_002065"
     CDS             17759..18337
                     /locus_tag="PWU81_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1110756.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18466..18843
                     /locus_tag="PWU81_002066"
     CDS             18466..18843
                     /locus_tag="PWU81_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDL1110757.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18776..20848
                     /locus_tag="PWU81_002067"
     CDS             18776..20848
                     /locus_tag="PWU81_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL1110758.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21059..22006)
                     /locus_tag="PWU81_002068"
     CDS             complement(21059..22006)
                     /locus_tag="PWU81_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL1110759.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22252..>22685
                     /locus_tag="PWU81_002069"
     CDS             22252..>22685
                     /locus_tag="PWU81_002069"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL1110760.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         7347 a         4143 c         4813 g         6382 t
ORIGIN      
        1 cagtaaaaat aaattaggta atactttaag taaacgcttt ctaatttgtt acgggaggaa
       61 tacacttgtt aaaaaaacgt ataaaataca gtgcgctaat gatgaatctc cttatgctca
      121 gtaccccggt tttaatttct gtcgttgaag ttgctgctaa cgaacaacag tctgtgaacg
      181 aagaaaatgc acacgtgaac attgaaggga tgtcaacaat ggaggaaata ccaagaacta
      241 gtgaagatat ggatacggaa gaaggaaatg atcctatgat gaaagaatca gaaaatgaaa
      301 agacaaaaaa gcaatctgaa gaagcaaatg aagagtggaa aggggttgtt ttcggagaaa
      361 gcacaagtgc tagtaggtat gccattgaac cattagaaga cggcgtgcgg ctctcttcca
      421 cgaataacgg cggaaaattc cagtcaagcg gatcagatgg aatgacctat tattatcaag
      481 caatccccaa agacatcaac tttaggatgc gggcgacgat cgaaatagaa tcttggacct
      541 atacgaatgc ccaagaagga tttgctttaa tggtaagaga cgcagtacca aaaaatcatt
      601 tgtttgggca ggcattttat tcaaattcat ttgccatact aggcagtcgg atcgaatatg
      661 tctgggactc ggatagacaa gaagtggtca acacgagcgg cagcaaatat acgatgcgac
      721 ttggattggg gacacgggca atcacaggaa tatcatctga agatccgcaa gctgcgccag
      781 ctcctgggag tgtctcagtg gaaacaacac ctttagagac ttcagcaggg ttcttggaaa
      841 aagaaacagg ttcttataat atattcggaa acggtcatgg gaatctgttt ccggccacga
      901 actcgattac taaactggac gtagagatga aaaaaacgaa tactggttta gaggcatatt
      961 attacgatcc tgaaacagga gaagaaatgg cttctgacat catgtatgac tgggaaaaat
     1021 tatacgcttc agatgaatcg gttatttatg caggatttgc tgcagcaagg aatatgacga
     1081 tcaaggtaga aggaatcgaa gttgcttact cagatcctgc gacagatcct cctgggcaag
     1141 aaagacctac tcgcttgatt gatccgattt acactgtgac ctctagtaat cattcaggaa
     1201 accaagatca tactatgtcc tttagagcaa atgctgacgg attattgaca attaaaaata
     1261 aagcaacagg agaggtgctt aagaacgcaa aaaacttggc tatcactgcg aatcgggaat
     1321 ttgattatca gacaaaactg atggaaggaa cgaacgaatt tactttcagt tttacgccag
     1381 ataaaaacta tccgccagaa gaatacgtag agatgactag ttatgaaaca aaagagattc
     1441 ttcatacagt ggactaccgt aaacctaaat attcgacagt ttatgtaaca ccagaagggt
     1501 cagatgcagg aaacggaagc agacaaaatc cattatctct gacacaagca ttgagatacg
     1561 catatgctgg gcagacaatc gtcatggcgc ctggaactta ttccttcgaa agaggattga
     1621 cgattccaaa aggcgttaac gggacaagag aaaacccgat tcagttgatt gcagagaaaa
     1681 atccagaaaa caagcgtcct gtacttgatt ttaaaggaac tggaaatggg atgacactgt
     1741 tcagtcacta ctggggttta cgagggtttg acattacaaa tagtgcagct atgcaaaaag
     1801 gattacagat ttctggaaat cataatttga tcgaagagat tcatgcttat cggaatggaa
     1861 atacaggaat ccaaatcagc ggtagctcaa atgatccttt cgaagcatgg cccgcgcata
     1921 acttagtcaa gaactgtaca tcttttgaaa atgccgatcc aggatttgaa gatgccgatg
     1981 gatttgcggc caaactgaca ataggagaag gaaatgtatt tgacggttgt atcgcgtatc
     2041 ataatgcaga tgacggttgg gacctttttg ctaaaaatga aacaggttcg atcggaaaag
     2101 taatcatcaa aaactcagtt gcttatcgaa acggctgggt accaggaatt gagggagaag
     2161 gtaacggtaa tggattcaaa atgggcggtt cttctttaac gggccctcat gaattgatca
     2221 atagtttgtc tttcgaaaat cttgcgaaag gaatcgattc caatagcgga acagatatta
     2281 tcgtcaatag cagtacaagt tttaataacg gttcatataa tgtggctctt tatacctctt
     2341 cagcaggaaa cacagactac catgcttctg gtatcttatc tttccgaaca gagaatcttg
     2401 agatgagaga gcagatccgc ccattgaatc aaaacgaaga tcaaatctac cactcgttga
     2461 actactattg gaatgaacga gagaaacaat cagaaaatac tcttggacat accatatcaa
     2521 aagattggat cgagtctcta aatacatgtt cgaaagaagg tcagcagcct gttacgcgct
     2581 atgaaaatgg aagtatccat gttcatggac tgatgcagat taaaccgggg aatcgtcaaa
     2641 cagaaaagga aagagtggat ggtcttccat tatctgtagg tgctattttt gacggagaaa
     2701 cctcaccgtc ttcggaatat ccagagtatg caatcgtaca agaaccaaat cagacaaaac
     2761 caaatgaaca aaataatccg attattatac aagaagcaaa tggtgtggcg gagaatgcag
     2821 aaggaaatat cattgaactg aagcctttta cagtttttgt gtcaactttc acaccaaatc
     2881 aaggaacgcc tgtctttgaa gatatgcgtt atttcccaga aaagatccaa atcgttgaaa
     2941 cacagtcaag tgctcaattt tctaatcata ccgctatttt ttcagcacct tcaaaaggag
     3001 aatatacgct agaagtcact tatcgttcgg aaatatttga tggggaaaaa tggtctgctc
     3061 atacagaatc tgttacccag caaaaagcga tcactgtgcg agaaactgcc gatgtgcccc
     3121 cgggaaatga caaaccagaa agagaaaatc caggaaatga atcaccaaat ggaaaaccaa
     3181 atggaaaacc ttctcatccg gataggacag caaaagaaag tgaaaaaaat ggcactcaag
     3241 gactcccaaa aacgggagaa tttaataacc cgttgctagc attagctgga ggaattttgt
     3301 taatcggtgt agttgtttat gtcatgaaac agcgaaaaaa ataagtggaa tttctccttt
     3361 tatgatgtaa aacaattgac tgaggaaagt aaaaatagaa ggaaatgaga ccaatagatg
     3421 tctcacttcc ttctatcttt ttatagatga ggttctcctt gctgcttttg attggctgtt
     3481 cttattactt gatgcaggac agctttttca caatctctcc actaaacggt gttcaatcag
     3541 ctgaccctcg gtattagctc aacaaacggc aaaatatgag gaactatttt tccgtttttt
     3601 tctctaatac tcaggtcagg acgctgattt cacaacctct cgataaacgg tgttcaaaag
     3661 ccagtttctc ggtagtaagt caaatttatg caaaaatgtg gaaagccatt ttcacaaaat
     3721 ttttcttact acttgaaact aagcagcttt ttcacaacct ctaatacatg gtattccaga
     3781 agtaacttct tcgaaaaata agccgaaatg tcacaaaaat ttgagaagcg attttcccaa
     3841 attccttctt atttcttgaa gctgaccact tctggcacaa cctcttacca gattcgcact
     3901 cttttttctg gcgctagata catagcgtct tgttccgtga taccaaatgt ttcatagaat
     3961 tcgtcaaggt tttttggttg cacgtttgct cggagtttag caggggcatg gacatcgatt
     4021 tgcagtaaaa gttgttggta ttcttttttt gcttttgtac gccaaatcgt tgcccaattg
     4081 acaaagaatt cctcgataga aagatctgat tccgtttttg ctgcttccaa tgcacagctt
     4141 agaccgcctg catcagcaat attttccgaa acggttaatt ttccgtttac ttttccgtca
     4201 gcaaaaggca gaccatcgaa ttcttcgatc attgcttgtg ctttgttttg aaattgtgta
     4261 agatcttctt ctgtccacca gttgttcaag ttgccatatt cgtcaaataa agcgccgtta
     4321 ttatcaaatg catgggaaat ctcatgagcg ataacagctc cgattccacc ataattagca
     4381 gagcttgatt gttctagact gtaaaatggt gcttgaagga tcgctgccgg aaagacgatc
     4441 acattccgaa atggatgata ataagcattg accgtgtcag cactcatttc ccattctgaa
     4501 cgatccactg gtttgttcca tttgctgaaa cgatctttta atgtgagttt gctgaaaaca
     4561 agagtatttg aaagcaaagt accaccctga tctgcaggta ctgttttgaa ctggtcgaat
     4621 aatgctggga tcttatcggg gtagcctact tggataccta agttatttaa ttttatgacg
     4681 gctttttctt ttgttgcatc cccaagccag tcattttctt ctagacgttt tttataaacg
     4741 gcgatcattt ttttcaccat ttgttctacg tcttttttag cagcttcgcc aaaatatttt
     4801 tttccgtagt aatcgccaac tacttgatcg aattgtccag aggcaaggta atacgctgct
     4861 tttttctgag gcattgcttc gtctgttcca gacaatacgc gagaatagat tccgctgact
     4921 tggcggaatt cttctgatag ataaccacta agagaacgta ctgttttaat gatcatccag
     4981 cttttgaata acggaaaatg agcttctgtc aggatgtcat tcaaatgatc gaagtacact
     5041 ggatcagtta cgatcacttt atctggagtt gtcccaatca aacccgtgac aagttttttc
     5101 aagtccagtt tatctgtgta tttaataaat gtatcaaaag attgcggatt atacattttg
     5161 ctgtaatctg cgttttcttc tgcacttttt acatgaggag caatcaattt atcaaattgc
     5221 actgcttgtt ccgtgattgc ttctgcctga cttttttctt tgccggcaag ctctaataac
     5281 tgaacagcca tatcaaagaa aacgcctatt agttgtgcac cgttgggatg gtctgatgca
     5341 taatacgtct tatctggaag gaataaagaa ggcggataag caaagaaggc gttcgtttgc
     5401 gcattcttca tatctgcatc tacatccaga ttaaatggca atggtaatgt atccaacagc
     5461 cattctggaa attgcttatt caattcgtcg aaagactgca atgattcaac tttttcgatt
     5521 ataggttgaa gcggttgagc atgcaactca tttcttcggt cataatcgtt tgccagttga
     5581 taatatgcaa ggaaatgttt cattttttct gaagacactt tttctggatg ggcaagcaga
     5641 tcgtctgttt ctcccatcaa aagttggtcg atcccgtcaa ctaaatcttg gaaaccgcct
     5701 gtagctggtt tatctgcagg gatttttgct gttttttgcc attcttcatt gactgcttca
     5761 tagaaatctt gtttcaatcg ttctttgttc attcatcgat cgctcctttt ttgtttcgtg
     5821 tgcccattat cttacaaaat gaaggaatta acaaatagaa cataaaaaat tgaaaattcg
     5881 aacaatcaaa ctgatttgtt tttttcaatc cttcacttat aatgaaaaaa aggaggcgat
     5941 cccaactgaa tcaagtagtg gataaacctt tatatttcaa agtagataaa aaaattaaaa
     6001 aattagccag cactcagcag ctgcagtcaa gaaaaagtaa attgcttttt ttagctctgg
     6061 tttttgaaga tcaaagttat gtgattatcg atcaatcagg acatccggta gaatattctc
     6121 cagcaaaata tacttatcaa gagggtcttt cttgtaaaaa atggaagctg atcaatgaag
     6181 agccaatcga gttatctaga tggatcaaca gaaaagaaga tattcccgtt ttgatcgaag
     6241 aaaaaaggtc aggaaaggaa ctcgcaaatt gctgggtggg attgccagaa gaacgttttt
     6301 tgaggtataa gaaatgggct accccctcag gctatctttg tggtacttac gcggcagcag
     6361 ttctccttgc ctattatcaa gactatcgta aggggtggat gcttccaaat gagattcgca
     6421 agaaaaatac ggctgattcg ttggttttga cgaaggcatt gcgtagccag atccaaccac
     6481 taggacttcc tacgattcct tttcaggtta gtacagggat ttctagtttt ttaaagaaga
     6541 atggcaatca tgagcgtgca cgagcaactc ttctaggttc atggcagcga gctacaaaac
     6601 gtatacgtga aggcaaacct gtgatgattg gtattttgaa agtacttggc agtacatatg
     6661 gcaatcattg ggtgacagcg tacgcttatt ttgaaactga aacaggagaa cgttattaca
     6721 aagtacacga caactggggc gattatcata aagtcattcc tgccagttgg agtaacggaa
     6781 cagtttcact tccttaagaa tttgctgaaa ttaacagaaa atcttggaaa aatatgttaa
     6841 actatcgaag aatagagaag ttttggggag gactacatga gtaataagta tcaggatgac
     6901 acgttgaaga tttttagttt gaatggcaac cgtccattag ctgaaaaaat cgcgaaagtg
     6961 ttcggaacag aattaggtaa aagcgtcgtg aagcaattta gtgatggcga gatttccatc
     7021 aatatcgaag aaagtattcg aggggatcat gtgtatatcg ttcaatcaac gaatgcacct
     7081 gtcaatgatt actatatgga actactgatc atgatcgatg caatgaagcg agcaagtgcg
     7141 aaaacgatca atgtcgtatt gccttactat gggtatgcta ggcaagaccg cacggcgaaa
     7201 cctcatgaac cgatcactgc gaaattgatc gctaacttga tcgaagaagc aggggcaact
     7261 cgagtactga ctttagatct gcacacggtt caagtacaag gattctttga tattcctgta
     7321 gataatttat ttacgatgcc gttgtttgct cattattacc gtcaattagg tctgacggga
     7381 gatgacatcg tagttgtttc gccaaaaaac agcggtgttc agcgtgcacg tagtctttca
     7441 gaatatttga acagcacatt agccatcgta gatcatgcag atgatgcttc aactgagggt
     7501 ggttatgtga tcggtgatgt tcaaggaaag acatgtatca tggtagatga tatcttgaac
     7561 acaggagcaa cgcttgcacg agcagcaaat gtattaaaag aaaacggcgc aaaagaagta
     7621 tatgcctgtg cgtcacatgg attattgtcc gatcatgcga aaagtatttt agacaatgca
     7681 ccaatcaaag atatttgtat cactgattct gttttcacag aagaaaatcg tcatccagaa
     7741 aacttaacaa tcgttacatg ttcagaatta atgggtgaag cggtcaaacg catccatgaa
     7801 aatacaccga tgagtccgtt attccgtctg gaagaaaaag aattcgaata aacaaaaaaa
     7861 agagggaaag aagttgtgac agaagtgagc agcttcaagg agctgcgaca agacttttgt
     7921 cacagctcct tttttctatg cccatgaaac gtcctctaga caaaaatgag ctcttctttg
     7981 gtttgttcgc tattttatgc tagaatgtgc ctagcaataa tttggaaggg atgaatgaag
     8041 tgaaaactat ttatctggat catgcggcaa ctacccccat gcatccacaa gtaattgaag
     8101 cgatgacggc aatcatgcaa gacacgtttg gaaatccttc aagcatacat ggatttggtc
     8161 gccacgcgca tgaaaaattg gaaacagcaa gacaaatcat tgcagacagt ctgcaagcaa
     8221 aacctcacga gatcatcttt aacagtgggg gaacagaagg ggataataca gctatcttag
     8281 aaacagcgtt ctcgcgtcaa aaagaaggca gacatctaat cactacagct atcgaacatc
     8341 ctgctgttct taataccatg aaatatttag agacaaaggg gtttgacgtt acatatctgc
     8401 ctgtagatga aaaagggaat ctgtcgatcg aagaggtcaa aaaagcgtta cgagaagata
     8461 cgatcttggt ctctgtgatg tacgggaaca atgaaatcgg aaatttgatg ccaatcaaag
     8521 aaatcggtga acttttagcg gatcatccag cttatttcca tacagatgcg gtacaagcgt
     8581 acggaaacca aacgatccat ccagaagaat taggaatcga tttattaagt atctctgctc
     8641 ataaaatcaa tgggccaaaa ggcgtcggtt tcttatataa acgagacgga gtaaatatcc
     8701 ctgcattgct tttaggcggg gagcaggaag aaaagcggcg tgcaggtaca gaaaatttag
     8761 cagggatctg gggtatggca aaagcagttg aactgctgtc acctgaagaa cgtgaagcac
     8821 gtaacaacaa atataaagga ttccagcaat tgattcttga tacattagaa gcaaacgaaa
     8881 tcccttatca agtaaatggt gatttaacga ataaactccc tcatgtgttg aatcttcgct
     8941 taattggcga agcaaatgat ttgctactga tgaagcttga tcttaatggg gtcgctattt
     9001 ctactggttc agcttgtaca gcaggaaata tcgagccttc acacgtatta gaagcaatgt
     9061 atggagaaga tacaccgata ctaaaagaat ccatccgtat cagctttggt ttgggaaata
     9121 ccgaagacga catccagtat ttccttgatc aattagttcg tgctgtcaaa aaataactag
     9181 tgaagtcatt cttatatgaa cagatgtctg agagaacgcc ttcatttttt agggaactta
     9241 actgaaaaac ctagtaaaaa tcaggataaa cttttataat aaagagaaac taattttatc
     9301 gaggtgaaaa tcatggcatt tgaaacaaca gcaacggtac tgggcgcacc aattagctat
     9361 cgtttgcatc ctgatgcaaa aagatataca ttgcgcgata atggatttac agaaacaaac
     9421 ggaggcaatt atcaattgat ccgaccatta gatgccacgc cgcaaagcaa agaaggattc
     9481 aaattaaaaa tcactgtatc aaaagatatt caaacattga aaatgtcgat cactacagca
     9541 aatggattga aagcagtcaa tattttcaaa gatccaaaac agaaaatgag ccaagataaa
     9601 ttttattttc tgatggatgg gatgattagc cgtgggttat tcgaaaaagt cgaatgatct
     9661 gtggatataa aaagacaact agttttgaaa cggcctattg ttagatgact aactttgtaa
     9721 ggacgacgca aattagttgt cttttttatt tttaaattta acacatgttg ttatttttaa
     9781 tattttgctg atttattgtt tctaagtaaa gaaatttaaa ttgaaacgat tgttatttag
     9841 ggaaaaaggt attttttgaa aaaagaatcc cgtataatga ttgttgtgca aacgctatac
     9901 aaaatgaaaa agaaaagagg gatgtttatg gaaaatgaac agtcggtcgt tttaacgaat
     9961 tggaagagaa attatttgtt ctttttatcg ggacagtttt tgtcaggaat cactagtatg
    10021 gtcgtccaat atgcgatcat ctggtatttg acaagagaaa ccggttcggc gacaattttg
    10081 agttttgcta ctttactagg aatgattccc atggttttac taagcccatt tgtaggaccg
    10141 ctagtggatc gctgggataa aaaagctctt ttgattgtca cggatattat tgtagcgatt
    10201 ttcgcgctta ttttagcagt agtcggaacg atttcagaat ccttcccgat ttggctagtt
    10261 tttgtgtctt tgtttatgcg ttcggtcgca caaacattcc aaatgccaac gatccagtcg
    10321 attatgccga caatcgttcc atcttctcac ataacaagga cgaatggaca gttaggaatg
    10381 gtccaatcag cgaatttcat cattgcgcca gctctaggtg cggctttgtt ttcagtcgtt
    10441 cctgtcaatt accttatttt attagatgta ttgggggcag tttttggtgt cggcttattg
    10501 atttttgtga agatcccaaa agtttctcct gaaatattgg aagttccgct tactattttt
    10561 aaagatgcaa aatttgggct gcagcaattg atggataata aagggttatg gtacataacg
    10621 atcaatggtg catttgtgat gctgttgttc atgccagcaa tcagtctgta tccattgatg
    10681 acgctagatt actttggtgg ttccgttgga caggcgggag cagtggaagt agtctacgca
    10741 gtcggaatgc tcttaggcgg tgcactgatc agttttatag gaacatggaa agaccgaatg
    10801 aaacctatta ttatagccta catcattatg ggcctgacga tcggtgcaag cggattggtt
    10861 ccaaacgata gtcaagggtt cttgtacttt cttatcttga atgcaggagc aggttgtgca
    10921 acgccatatt tcaatacact gctgatggca atgatccagc agagttatga atcgaatgtt
    10981 ttaggtcgtg tcttagggaa cttcaactct ttgatgaacc tggcaggtcc aattggatta
    11041 ttatttgcag gaccattagc tgatcgttta ggggtagaaa aaatgttctt gttttccgga
    11101 atcgggattt tattatgcgg aatcgtttta tttttaacgt cagctgcccg aaattatgat
    11161 aaagaattac agaaaaaact ggtaaaagaa caccatgagc aaaaagacga atagtatgga
    11221 atggagttgt gacagaagtc ttgttacaac tccatttctg atcctaatgt tcctgtggca
    11281 ataagcctac tcaaatgaaa aatatgagta attatcttta tttgtttaat acttgatata
    11341 gggcagtttt ttcacgacct atcgtacttc ctctttgctt catagatttc tttgatttgt
    11401 tgctcagata acggagtgcc aaatggtagt ttttccttta agagttctct ttccggtgcg
    11461 tctacgccta cattgacatc gttctcgata ggtacgctgt aatgatggat atgtccatga
    11521 agattgcgga tagtgtcgtt aggcccgagc aacatcggat aatgagtcaa gtaatattgt
    11581 tgatggtcga atttaacgat ggcacctaca tcataaaaat aaaatttggg acttccgtca
    11641 gtaagcctag gatcattttt ttctaaataa cggaaaaaag cgcgactgtc atggttccct
    11701 ttgatcaagt ggattgttcc ttgcagttga gtgaccagtt ccaaaatttc tgcatttgct
    11761 ttttcatatt gcggatgcat agcaaggtcg cctagatgat aaacatggtc ggtttctttt
    11821 attactgcat tccagctagt gatcaactgt tggttcattt catccacatt tgcaaagagc
    11881 cgcggagcaa agtcgttttt tcctaataag cgttcatgga aaaagtgcat atcactgata
    11941 aagtaattca tgtatctttt gcctcccttt tttcttagag taaacaaaat cacttatctg
    12001 agcaactcat aacatctaaa aagaaaaggt tgtaaatgag acgtgcagct ttaagcgata
    12061 agaagcaatt ttgaaaaata gtttttcaca atttataaaa ttattcctta ttgccgaaag
    12121 gctggccctt tttatgagta tgccaatttt ttatatcgaa tgctcaaaag atgagtgatt
    12181 ttttggttga ctatcatgtt tgtgtactga ataggtccag tagaacagta tttttacgac
    12241 ttgataatta agggatgaaa ttttcagaaa tatgacgtat aatgtaaaat actgaaaagt
    12301 tgcgaccttc aactcgtaga tgtttcagaa tctaattgaa atgatggtga tatcatgaca
    12361 gacaacagca aaacacgcgt tgtcgtaggg atgagcggcg gtgtcgactc atctgtaaca
    12421 gcacttttgt taaaggaaca aggatacgac gtgatcggaa tattcatgaa aaattgggat
    12481 gacacggacg aaaacggtgt atgcacggcc acggaagatt ataaggatgt agcgaaagta
    12541 gctgctcaaa tcgggattcc ttattactct gtgaattttg aaaaagaata ttgggatcgt
    12601 gtatttgaat atttcctagc cgaataccgc gcaggacgta cacctaaccc agacgtgatg
    12661 tgcaacaaag aaatcaaatt caaagcattc ttggattatg caatggatct tggcgccgat
    12721 tatgtagcta ctggacatta tgcgcaagtc actcgcgatg aaaacggcgt ggtccacatg
    12781 cttcgtggag tagacaataa taaagatcaa acttacttcc ttagccaatt atcacaagaa
    12841 cagctttcaa aaacgatgtt ccctctaggc ggcatggaaa aatcagaagt acgggcaatt
    12901 gctgaacgcg caggccttgc gactgctaag aaaaaagatt caacgggaat ctgctttatt
    12961 ggtgaaaaga acttcaaaca atttttgagc aactatctgc cagcaaaaaa aggaaatatg
    13021 gtcactcttg atggtgaagt caaaggacaa catgctggct tgatgtatta tacgattggt
    13081 caacgtcaag gactagggat cggtggcgga ggagattcgc aagaaccctg gtttgttgta
    13141 ggaaaagatc tagcgacaaa cacattatat gtggggcaag ggttccatca tcctgcattg
    13201 tatgccacaa gcttggatgc aagtgaaatc cactttacga cgaatgaacc aatgccaaaa
    13261 gaattcaagt gtacagcgaa attccgttat cgtcagcaag acgttcctgt gacagttcgc
    13321 ttattggatg ataatcgtgc agaagtcgta ttcgacgaac ctgtacgtgc aatcactcct
    13381 ggacaagcag ttgtatttta tgatggaatg gaatgtctgg gtggtggttt gatcgaccac
    13441 gcatatcaag aaacaaaagt tttacaatac gtttaagcga tattaaatga atcaaaacag
    13501 ctatggctaa caaaagaaac accttttctt ttggatagca tagctgtttt tttgcaataa
    13561 aattaatttt tattcatttt atacttgaaa tacttgtgta aaaagttcat aatatccaat
    13621 aatatgaata aagttgcttt ttgttaagca agagcagttg caaaatagaa gggaatcttt
    13681 tgcaagtgat ttgttcaatt ttataagtga gggtgatgga aatggaaaag aaatcgacgg
    13741 gtattagcaa atggggactg gttgctctgg ttgtcagttc gtcaatcgga agcggtgtgt
    13801 ttgggatcac aagtgatctg gctagttctg cagctccagg gccagcgatc ctttcatgga
    13861 taatcgttgg gattggtatt ttagctttgg tattatcgct gaatcattta ggagaaaagc
    13921 gtccagatct agatggtggt attttcggtt acgcggaagc ttcatttggt aaattaggtg
    13981 gcttcatcag tggttggggt tactggttgt ctgcgtggct gggaaatgtt gcttttgcaa
    14041 cgatgctgat gagcgccatg ggagaatttt tcccgatatt caaaggtgga caaaatgttc
    14101 cttctatttt atttgcaagt atttttattt ggggtctgac tttattagta aacaatggag
    14161 ttgaaggagc gagcgtcatc aatacaatcg tgacgatctg taaattagtg ccattattcc
    14221 tatttttaat atttgtattt atcgcattta aagtgaatat attcactgct gatttttggg
    14281 gtaacgtatc tgataatcta gtgaatggaa cagggcagca aggaacgatc tggttgcaga
    14341 ttaaaggttg tttgatggtt atgatgtggg tctttgtagg gatcgaaggc gcaagtgttt
    14401 tagccaaccg cgcgaagaaa cgttcagaag cccagcaagc atcgatcatt ggtctgcttt
    14461 gtttattgtt tatctatatc cttgcttcga tcttgccata cggcgtctta tctcaagaag
    14521 agttagcaac tatttcacaa cctgctatgg caaatatttt aaaaggaatc gtgggaaatt
    14581 ggggagcagc gctgatcaat atcggcttga ttatttcgat cattggttct tggttgtctt
    14641 ggacgatgct cccagcagaa acaacgatgt tgatggctcg agacggtctg ttgccgaaag
    14701 gttggggaaa aacgaataaa aaaaatgccc caacttattc gttagttgtt actgcaatct
    14761 tgactaatct attcttattg actttcttag ttactgatta tgcctatcaa tttgcctatt
    14821 ctttatgtac agcggcaatt ttgatttgtt atctacttgt cgggatctat caagtccagt
    14881 tttctttaaa agaaggaatc aaaaaacaag tagtgatcgg tgggattgct gtagtttttg
    14941 aattgatggg catcatttta gcaggatttt cttatgtcct tttatgtagt attgcctatc
    15001 ttcctggttt ttgcttctac tggatcgctt gtcgtgaaac caaacatcaa attacgggaa
    15061 aagaaaaagt aatgattggg ctgatttgtg tggccgcact gctttcaatc gggttgcttg
    15121 ctgtaggctg gatcaagata taagggggaa agaaacatga aaatcgcaaa tttcggtgta
    15181 gaagaatggc taaataaatg ggaaaagaaa gcggtatatg atattgcaca aagctcgatc
    15241 gaagcattga cattagacga actcgttggc ttagatggga ctagcgtaag taaatttttc
    15301 gaaaaacgca aaaacgaacc attggactat ggctggatcg aaggatcaga tgttttcaaa
    15361 caagaagtcg cttctcttta tcaaaccgtc gatccggaaa acatcttaca aacgaatgga
    15421 gcgactggtg ctaatctttt agctctatac gcgttagtgg aagcaggaga ccatgttgtt
    15481 tcgatgcttc ctacttatca gcaattatat gacatcccca aatctttagg tgccgctgtt
    15541 gactttgttc atttgaagga agaagaagat tggcagtttg atttagaaca gctagaagcc
    15601 ttagtcaaac ctgaaacaaa aatcatctgt ttgaatagtg caaacaatcc tacaggcaca
    15661 ttgctagatc ggaaaacttt agaaaaaatc gcagaaattg ctcgtacagt agatgcatat
    15721 gtattgatag atgaagtata tgcgccttta acggataaag gtgaattctt gtcgatcgta
    15781 gatgtctatg aaaaaggtat agcaaccaat tccctatcca aaacctactc cattccaggt
    15841 attcgtattg gctggacagc tactggacct gagttggcag aagtttttcg aaagtatcga
    15901 gactacacga tgatttgcgg cggagtcctg tcagatgatc ttgctgtaca tgcattgaag
    15961 aataaagaaa aaatattggc gcgaaacaaa aagatcatta cagagaacct tgtgatttta
    16021 aaacaatggg tagccaatga accgaaagtt gaacttgtcg ctcccaatta cgtctctact
    16081 tctttcatta aattagccat cgaagaagat gaccagacat tttgtatcaa tttattagaa
    16141 gaaacaggag tactattagt accaggaagt gcctttgatt taccgaagca tgcccgacta
    16201 ggttactgtt gtaaaaaaga aaccttggaa aagggattat tcctcttatc tgagttcttg
    16261 aaaaaacagg cctaaacaaa aacactgcaa ggacagtctg tgacaactgt tcaagcagtg
    16321 tttttcattt acgcttattg tctatgagaa ggagagtgcc ctgaagctat ggagactcct
    16381 gaagcttttt cttctgcagt ttcttgctga tggatctctt gtgtattgtg tcgtacaagc
    16441 aagaagttga ccaaaacaag aagagaagtc gaaagaaaga cgccgcgata accaaatgca
    16501 ctggaaacac tggagccgat cataggccct actacgttac cagttgcttg gaaagattgg
    16561 ttataactga agatccgtcc agcagcttga tgaggcgaat attttgtgat caatgcttgg
    16621 acagcaggga gcagacaagc atcagagata ccaatcaaaa aacgtaaggc agctaattgc
    16681 caaacattcg tgacgaatgc cattgggata taaaccagaa tcgcaaaacc caaaccaatc
    16741 gcaagtattc tttcactccc aatcttgtca cctaagcgac caaatcttgg agcggctatc
    16801 aaagtggcaa tcccaggtat cgaagcaatg attccgctga tcaacgtaac attcccatgt
    16861 ccatgcagca attggcgaat gtacagactg atgattggac taatggaatt gttggatgct
    16921 tggatgatca tcgttgtgac gaacataccg atgacgacgt gtggatattt caagtctttg
    16981 aaaatctgtt tggcagaaac catgttttct tttttcactg ggacaaattt ttcatggacg
    17041 aaaaacagac tcaacaagaa gacgaaaaat aagatgatcc ctgtaatgaa aaaggtaggg
    17101 cgatagccaa acgctgaagc agagatcccg ccaagaagcg gtccaagtaa tgttcctgta
    17161 acagaaccag ttgctaaggt tccaagaact tgaccacttt tttcttttgg cgtcccagtt
    17221 gcgacaaggg cagtggcatt actgatataa ccagaaaaaa tcccttgaag cagacgcaat
    17281 gcgactaatt gatacacact agtcacgcaa cccatcaaag aaatgacgat tgccattcct
    17341 aatgaagcac gcaacagcat caattttctt cctttttggt cggctaattt tccccaccaa
    17401 ggagagataa gcgtagtcac caagaaagta gatgaaaaag tcaatccgct ccaaaagttc
    17461 aattgtgacg tactataatt acctaacgta tctatgtaaa gtgacataaa aggcatgact
    17521 aaactaaatc cgataccagc cataaatgtt ccaaaccata agacaaacaa gttttgttcc
    17581 caagtttctc ttttcctaaa taatttttcc tttattcttg cagccaatta gactccttct
    17641 ttctgaaaat tttcatctac agctctttat tataaactac aatatgtagt ttatcaatga
    17701 aagtctcttt tttctctttc tttttcgtgg taaactgaaa gagtgaagga tttattttat
    17761 gagaggaaac atggaaatga aaaaaagaaa tctgtctcaa gagaaaatca tcgactgctt
    17821 tcgagaacta gcagaagaga tggatgttca gcaaataacc tttcagcatt tagccaaacg
    17881 cttggatatc aaggctccct cgctgtataa ccatttcaaa aatattcgtg atgtcaaaac
    17941 agcgctaaca gcgaaacttc tgcaggaact taatgaccag ctgcgacggg ctcttgtggg
    18001 aaaaagtggt caggaagcac tgcagatcta tgcagaaact tatcgatcct ttgctttttc
    18061 aaatcaagca gtgtatgagt tactgatcag tgtaccgcat accaatgaag ctgttttatt
    18121 agatggaatc catgaaacga atcagatcat tttgcagata tttgaggctt ttcaattaac
    18181 gaaagaagaa aaagttcatc gaagcaggga acttcgtagt atgatccacg gttatctttc
    18241 cttacgtttt ttaggatact ttacaaaaga accaacaatc gatccagaag acagctattt
    18301 ttggatgatc aataacttta tcgctacttt gccttaaaca atcagattca tttgtaacaa
    18361 ttttaagatg ttcattaaat gaccctttta taagagaaaa ttcttgcaac tttcttgaaa
    18421 agtgactaag atgagtctag aaaagctgaa agaaggagca cgagtatgta tcaagtaatt
    18481 accatgttcg gggacaatga gccttggtgg ttttttgaag actgggaaga agatattgaa
    18541 gaagaagaaa cattcgagtc tttcgaagaa gcacgacaag cgtacgaaaa acaatggaac
    18601 gaaatccatc aaaattatga atatatcaat gcaaaaagta actttctcag cgccttttgg
    18661 aatgacggag atgagcgctg gtgtgaagag tgtgacgatg atttacagca atacaaggga
    18721 cttgcgttgt tgaaaaatca tcagcccatc acagtagaaa gcagaaagga attttatgaa
    18781 acaactaatt ctagcggaaa aaccaagcgt tgcgaaagac ctaagcaagg tgcttggtgc
    18841 taatcaaaag cacaaaaatt attatgaagg acccaaggtg atcgttactt gggcccttgg
    18901 tcatttatta ggattgaaaa tgccggaaga tctgaataag gaatggcaga gctggcaaat
    18961 ggacacgttg ccaatgatcc ctaaaaaact aggaatcaaa ccacttccta aaacaggaca
    19021 tcaattaaaa gcaatcaagc agctggctca gcgaaaagat gtctcagaag cagtcattgc
    19081 gacagatgcc ggaagagaag gggaacttgt tgctcgctgg atcttagagt atgtgcattt
    19141 caataaacca gtgaagcggt tatggatctc ttctcaaaca gataaagcaa tcaaaactgg
    19201 attcaagcaa ctaagacctg cgaaagaata tgatgcattg tatgaatctg ctcttgcccg
    19261 ggcaaaagcg gactggctgg taggattgaa tgtgaccaga gccttaactg tgaagtacgc
    19321 ggataattta tcagcaggcc gtgtgcagac accgacacta gcattagtga gagaccaaga
    19381 aaagaaaatc gagcagttcc gtccacaaac gtatttcacg atcacgttgc aagctggaaa
    19441 agaacaagcc aaaatgcttc aaaaaaatca atttgcttta aaaacacaag aagaagcaca
    19501 aaaacttgtt caattgatca gtacaagcaa aggaactgtc acagaagtcc aagaaaaatt
    19561 aaaaacggaa tctgcgccat tgccttatga tttaacagag atccaaagag aagccaatca
    19621 acgctatggc tattctgcaa agaaaacatt aagtcttgta caaagtttgt atgagaccca
    19681 taagatcgtt acttatccgc gtacagacag caaatatctg acaaatgaca tgaaaagtac
    19741 gatgaaagaa cgattgcaag cagtcagcga ctttgctcct gaggtcaagc agtatttgaa
    19801 aaacggagcc atcgtccaac agacaaaagt attccaagac agtaaagtga ccgatcacca
    19861 tgcacttttg ccaactgaaa acagagcacg ttatgaaaaa ttaagtaatg aagagcgaaa
    19921 aatctatcaa atgattgtct ctcgtttttt aggactgttt gctcaacccc ataaagtcag
    19981 ccaaacaaaa gttacggttg aatttggtaa agaacgattc atttttcggc aaaatcgtgt
    20041 cgtccaagca ggatggaaag gcgaaactga aactgagacg gaaacagtca aatgggaaaa
    20101 aggaatgaga atcaatcccg atttcgccat caaaaaagaa ctatctgcac cgcctaaacc
    20161 attgtcagaa gcaagcttgc ttggtcaaat ggaaaaatac agtctgggaa ccccagctac
    20221 gcgtgcagag attatcgaga aactaatcaa atctgaactg atggaacgta cccctcaagg
    20281 actgcaagta tctcctaaag gtaagcaatt attagtattg gtaaatccct ctttagtaac
    20341 accagatttg acagaaagct gggaaaaaga ccttgaagct attgctgcag gaaagaaaca
    20401 agcaggaacc ttcttgaatg gaatcgaaaa agaaacaaaa cgcttggtga atgagatcaa
    20461 atcaagcaaa caagaatatc aagatttctc gattacccaa aagaaatgcc cagaatgtgg
    20521 ggccaatctg cgcgagaaaa acacacgtga cggcaaaatc tatgtctgca ccaattcaga
    20581 ttgcaactac cgcagaagaa aagatccaaa agtctctaat catcgatgcc cgcaatgtca
    20641 ccggaaaatg gaaatcatcg aaggtaaaaa cggtgcttat ttccgttgta aatacgatgg
    20701 aacgacagaa aaattgcttg ataaaaaaga gcagaagaag aaaatgacca aacatgaaga
    20761 acgacgcttg ctgaaaaaat atgcacaagc agaagaacca gaagaaagcc cattagcagc
    20821 agcactaaaa gcagcaatga agaagtaata agatatgaaa gaggttgtga tagaagtggt
    20881 cagcttcaag aaataagaag gaatttacga aaattgcttc tcaaattttt gtgaaatttc
    20941 ggcttatttc cgaagaagct acttctggaa caccatttat tcggaaaaaa ggactgtgac
    21001 aagacttttg tcacagtcct ttttcattta aaaaaatgtt tacttcattt gacgtatatt
    21061 attggtgagg cttcaattca tttggcttca ctttttctgt ttctcttatg atataaacag
    21121 gacgctgttt cgtttctaag aagattttcc caatatattt cccgatgatt ccaagacata
    21181 acaattggat tccaccaatg aacaggaaga tcgagaccat ggaaggccat cctgatgtag
    21241 gatcaccaaa caacaatgcc cgcaaaataa tgaacaaaat tgcaatacca gaaccgacac
    21301 aagacaaagc gccgataaat gaagcaatcg ttaacggtgc atccgagaag ttcacgatac
    21361 catcaatcga gtagttgaat aatttccaga aggaccaaga tgtttcgcca gccacacgtt
    21421 cgcggttttc ataagaaata tattctgttt taaatccgac ccagctaaat aatccttttg
    21481 aaaatcggtt gtattctgtt aattctagga cggcatccac catttgacga gtcatcaagc
    21541 ggaaatcacg agcgccatcg accatttctg tatcgctgat tttattgatc aactggtaga
    21601 attttttcgc aaagaaactg cgtatcgcag gctcgccttc acgagtcgaa cgccgggtgc
    21661 cgacacaatc gatatcagaa gtagtgatcc ggttgatcat ctcaggaagc agttcgggcg
    21721 gatcttgcag atcaacatcc atcacggtca ctaaatctcc tgtagaagct tgcaatccag
    21781 cgtacagagc cgcttctttc ccaaaatttc tggaaaatga gacaaagcgg acataaggat
    21841 aagctgaggc tagcttcttg attgctgcta acgtaccgtc ttttgatccg tcgtcaataa
    21901 acaaatattc gaactcatgg gcacctttga atttttcaac ctcatcaaaa aaaagaggaa
    21961 cagttttttc ttcattaaaa catgggacaa caattgatac tttcatcgtt tctatccttt
    22021 caatcgtatg tttatatatg gtttacattc aaaatttaat aaaccttaaa taacaccttt
    22081 aaaatttgat tttacgtaaa agaacaggta ttctttttgt aaactaacgt atttagttta
    22141 ccatgtttcc tcggtttatc aaacttttaa gaacaatttt tattagttgg caagtagttt
    22201 tggatagaga aaaaggagga agttccagtc tattgactag gagcaaacaa aatgaaaata
    22261 cacacaccta tcggcttttt atggttagat gcagatgaat ccggattgac tgctgtatct
    22321 tttcagccaa tttcagaaag cgaatcagcg aatcagcgaa ttttaacgcg cacacaagaa
    22381 cagtttgaag aatattttgc gggaaaaaga aaagaatttg atgttccttt atctataaag
    22441 agaggtacgc cttttcagca aaatgtctgg aaagctttgt cgaccattcc ttatggagaa
    22501 atccgaacgt ataaagaagt agccgttgct gttgatagtc cgaaagctgt gcgagcgatt
    22561 gggcaggcaa atcgaaccaa tccattgcca atcgttattc cttgtcatcg agtgatcggg
    22621 caaaatggaa aactgacagg atatatggga aacgcaatag atggggttga aatcaaacgc
    22681 caatt
//
LOCUS       DANJMI010000043        22633 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000043 DANJMI010000000
VERSION     DANJMI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.043"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(227..1114)
                     /locus_tag="PWU81_002070"
     CDS             complement(227..1114)
                     /locus_tag="PWU81_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1110761.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="PWU81_002071"
     CDS             complement(1111..1980)
                     /locus_tag="PWU81_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1110762.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="PWU81_002072"
     CDS             complement(2117..3403)
                     /locus_tag="PWU81_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL1110763.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="PWU81_002073"
     CDS             3676..4254
                     /locus_tag="PWU81_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL1110764.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..5183
                     /locus_tag="PWU81_002074"
     CDS             4254..5183
                     /locus_tag="PWU81_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1110765.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5180..6916
                     /locus_tag="PWU81_002075"
     CDS             5180..6916
                     /locus_tag="PWU81_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL1110766.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="PWU81_002076"
     CDS             complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="PWU81_002076"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDL1110767.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7812..8321)
                     /locus_tag="PWU81_002077"
     CDS             complement(7812..8321)
                     /locus_tag="PWU81_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDL1110768.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="PWU81_002078"
     CDS             complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="PWU81_002078"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL1110769.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9622..10713)
                     /locus_tag="PWU81_002079"
     CDS             complement(9622..10713)
                     /locus_tag="PWU81_002079"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDL1110770.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="PWU81_002080"
     CDS             complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="PWU81_002080"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL1110771.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="PWU81_002081"
     CDS             complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="PWU81_002081"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL1110772.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="PWU81_002082"
     CDS             complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="PWU81_002082"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL1110773.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14676..15650)
                     /locus_tag="PWU81_002083"
     CDS             complement(14676..15650)
                     /locus_tag="PWU81_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HDL1110774.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15631..17514)
                     /locus_tag="PWU81_002084"
     CDS             complement(15631..17514)
                     /locus_tag="PWU81_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HDL1110775.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17532..19757)
                     /locus_tag="PWU81_002085"
     CDS             complement(17532..19757)
                     /locus_tag="PWU81_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL1110776.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19872..20906
                     /locus_tag="PWU81_002086"
     CDS             19872..20906
                     /locus_tag="PWU81_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1110777.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20973..21986)
                     /locus_tag="PWU81_002087"
     CDS             complement(20973..21986)
                     /locus_tag="PWU81_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL1110778.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22064..22252)
                     /locus_tag="PWU81_002088"
     CDS             complement(22064..22252)
                     /locus_tag="PWU81_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL1110779.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         6446 a         4863 c         4096 g         7228 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa gaaatcaatt
     4921 tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc atctttactg
     4981 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
     5041 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
     5101 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
     5161 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
     5221 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
     5281 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
     5341 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
     5401 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
     5461 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
     5521 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
     5581 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
     5641 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
     5701 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
     5761 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
     5821 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
     5881 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
     5941 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     6001 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     6061 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     6121 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     6181 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     6241 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     6301 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     6361 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     6421 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     6481 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     6541 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     6601 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     6661 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     6721 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     6781 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     6841 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     6901 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     6961 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     7021 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     7081 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     7141 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     7201 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     7261 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     7321 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     7381 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     7441 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     7501 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     7561 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     7621 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     7681 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     7741 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     7801 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     7861 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     7921 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     7981 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     8041 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     8101 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     8161 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     8221 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     8281 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac
     8341 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     8401 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     8461 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     8521 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     8581 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     8641 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     8701 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     8761 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     8821 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     8881 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     8941 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     9001 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     9061 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     9121 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     9181 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     9241 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     9301 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     9361 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     9421 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     9481 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     9541 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     9601 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     9661 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     9721 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     9781 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     9841 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     9901 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     9961 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
    10021 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
    10081 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
    10141 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
    10201 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
    10261 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
    10321 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
    10381 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
    10441 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
    10501 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
    10561 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
    10621 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
    10681 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
    10741 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
    10801 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
    10861 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
    10921 cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
    10981 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
    11041 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
    11101 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
    11161 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
    11221 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
    11281 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
    11341 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
    11401 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
    11461 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
    11521 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
    11581 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
    11641 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
    11701 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
    11761 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
    11821 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg
    11881 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
    11941 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
    12001 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
    12061 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
    12121 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
    12181 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
    12241 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
    12301 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
    12361 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
    12421 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
    12481 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
    12541 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
    12601 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
    12661 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
    12721 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
    12781 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
    12841 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
    12901 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
    12961 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
    13021 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
    13081 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
    13141 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
    13201 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
    13261 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
    13321 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
    13381 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
    13441 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
    13501 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
    13561 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
    13621 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
    13681 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
    13741 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
    13801 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
    13861 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
    13921 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
    13981 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
    14041 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
    14101 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
    14161 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
    14221 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
    14281 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
    14341 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
    14401 agttttctag atttcataaa aaatcgctcc aaactgatgt aagaattgta cctagtatag
    14461 acataaaaaa atcgactgtc aatcattttt atgaaacttt ttttaatttt ctgatagtta
    14521 actttgtttt tcagaaaaat aacaattggt tttattgaag aaaatagtca tgaacaaaag
    14581 tatctgagaa cttttttact ctcttgttca tgacatatag aataaagcag ctttgtcttg
    14641 taaactccgt ttttcaaata ataatctaaa gattgctact gaataataaa gccgatttca
    14701 tggtcctctt cgctgctgat tcggtaaggt gactcaacat ccgctcccca gctattgata
    14761 cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg ttcgtctttc tggcggtagt
    14821 tcttctatat gtgttgcctg ttccagttct tctgctttga atggtaaagc ggaaaaagca
    14881 aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt cttgatcttt atccgtatta
    14941 tccaacgttt gatttcttgt tagttctacc catttcgttt ccatatgcat gccgcattct
    15001 tggggaacaa ggtatgggga aacggggagt cctgccactt tataaatacc ttcgacacca
    15061 ccagccatcc gatccggata tgtttcccct gataggcctt catactcaaa aaattttgct
    15121 ttcgttggca taatgaacct taaacccaat gcgggtaatt ccggtaatcc tttttgtcca
    15181 taataatgca tattgacaag cattttaccc tctccagcga tttggtagga aattgtcact
    15241 tgggtttttg gtactgtgtt gatttccagt gtaaaaatga tttcaaccgt ttcagcgtat
    15301 tcattacttg aatattgatt atttactgga gcaatcggca aatcgatctt tttattgtcc
    15361 accagtactt ctatttcttt gacttttgga tacaatccgg ctcctaacca cataccggat
    15421 ttaaatccaa atccgctacc tctgtcatta tcggtcgttg ctcgccaaaa aactggaagt
    15481 gtttcccggt atagccattc ttttccgttt ttattgagcg attccagtcc gcctcttgta
    15541 taagaaaaaa tataatgcgt attccccgat ttgacaccta atgtacagtc gccgaagacg
    15601 attgcaactt tttccgtatc catatgactt ttatttactg tatttttcat aataatatct
    15661 tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa acgatcccat tccctgagaa
    15721 ttcatagtca cttggtttat cgtcaaagtc accgccatag cgcatcactt ctctgcctgt
    15781 gattggatca gtcacttgga tcgcctgatc tttaaaatcc catatatagc ctccttggta
    15841 catttcataa cgatcaagca acgtcatgta agaatccatt ccacctaacg aattccccat
    15901 gtcatgcata tattcacata agatgaacgg tttttccgct ttattttcaa ggtagtcaat
    15961 gatctcttct ggtggcgcat acatgcagct ttttacatct gaaatcgaat cttcgaatgc
    16021 tggatttgga aaattccctt cataatggac aagtcgatcg gggtctactt ttttgaagta
    16081 atcgttcatt tgtcgaatgt tttcttccgc ataagattca tttccaagag accaaaaaag
    16141 aatacttgga tggtttttta atgtttcgaa attcgttttt gctcgatcaa gaacagccgc
    16201 ctgccactga ggtactgcac ctggtacgtt gtaagaaggc tcaaatgcgc ccattttttg
    16261 ccatgagcca tgggattcga ggttcgtttc cgccatcaca tggattcctg cttggtcgca
    16321 taaataatac caagtgatct ggttggggta atgagatgtc cgtacgccgt tgatctttgc
    16381 attttttatg atagccaaat ctttttccat atcttctata gtgatggcgc gtcctttttt
    16441 cggatgccac tcatgtcgat ttaccccgtt gataatcatc cgttttccat tgaagtagat
    16501 gattttatct ttgatttcga ttttacgaaa accgaaggaa tagtcaatat actccgttac
    16561 tcttttttct tcgtctatcg tctccaattc aagcttatat aaattgggct gaaaatgccc
    16621 ccacagtgcc acattttcaa atccatcgat gcgaatcgtc atttgctctt ttaacggata
    16681 ttgctgttga tagatttcct tcccatcagg atctagtatc gctacattca gctgactgtt
    16741 tttggctact tgctcaaaag acaaatctac ttcaagtttg ccattttgac atttgtcatc
    16801 taagcgaggt ttgatccaaa gatcttctaa atggttttgt cctcgagcaa ctaagaagac
    16861 atctctgaag atcccaaaaa atcggaaaaa gtcctgatct tccaagaatg cagctgtact
    16921 tcttttgtaa acctccacag ctagaatatt ttcttcttcc aaataaggtg ttaaatcaaa
    16981 ttcagacgga gtgaacgaat cttcggcata cccgataaaa tgaccattca accaaataaa
    17041 cattgcttgt tctacaccag caaaatgaat cgagatttgt tttcccttta gaccttcttc
    17101 taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct ttagcagctg aaaagatccc
    17161 ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg cgaaatacat gtccctccca
    17221 cggatacatc gtattgatgt aatggatctt gtcataattt gctaattcaa tatgactagg
    17281 aacagtgatt tcatcaaaag aacaggcatc aaaatctttt ttataaaatg tttttggtcg
    17341 taccgaagga tttggcgaat aacaaaaacg ccatgatcca tttaaagact gtttaaggga
    17401 ggatgtggtt tctacttctt cccttgttgc ataacaaaca tgatcgctat gtgcatttga
    17461 ttggttcact ctaaaaactt gtggatcatc taaccatttt atatttgctt tcatactttt
    17521 ttccttctct ctcataactt tttgaaatga agcagttctg tttggaaatc accttgaaaa
    17581 tcggggaagt aaagtccaac tgtcatcaat tcggcccccg aaaatatccg cttcgaggtc
    17641 tgttcctgat aaagtgcctc atcatctaac cccatcagat atacttcatg gaacgctggt
    17701 tgagctgaag ctagtatcct ccctaaaaac acaatagctt catcttgttt aggagacaca
    17761 aacatccatg cggcaatatt ttcttcaaaa ggactcgcca aacgataata atggccatac
    17821 tggatcaccg gacgaatcgt ttgataggat ataatttggt cttttacttg ctttttttct
    17881 tcaggagaca gctttgtcaa atccagttca taaccaaaaa cagcgctcat agctgtttct
    17941 cctcttgttt tcaacgaagt agatctgtcg gtctgatgat ttggtacagc tgatacatga
    18001 gctgtgatgg acgaaatcgg ataagcaagg cttgttccat attggatttt catacgctcc
    18061 actgcatccg tgttatcact tgtccatgac tgcggcatat aataaataaa cccggcatca
    18121 aaccgtccac ctccaccaga acagccttcc cataagattt ccggataatc agttgtcaaa
    18181 tgttcaagta gttcataaag cccgagcata taacgatgcg ctacttctcc ttgtctctgt
    18241 gggtcaaggg taatcgaata gacatctgaa agcgaacggt tcatatccca tttgatgtag
    18301 tcaagaggaa tcgtatctaa aatctttctc atttgtttct caatcgtctg ccgaacatct
    18361 aaccttgtaa aatccagtac atgttgtgat ctggaagcag aaggcattct acctggctcc
    18421 tgcataagaa aatcaggatg tgttcgatac agttcagaat caaccgagat catttcaggt
    18481 tcaaaccata aaccaaattt caatcctttg ctatgagcat aatctgctat ttcacgtaat
    18541 ccatttgtca acttgccttc atactcgaac cagtctccta atgaagaatt gtccgcatct
    18601 ctttttccaa accagccatc atctaatacg aacatttcga tgcctagctc ctttgcttca
    18661 tcaatgatcg ccttgatttt ttcactattg aagtcaaagt aagtggcttc ccagttattg
    18721 accagaatcg gccgttcagc aaattgatga cgacctcttg cgattctttc acgtagcaac
    18781 tcatggtgga tctgactcat tttgttcaat ccctgacttg agtaagaaag gatcatttca
    18841 ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa aacgatggct gttgatccca
    18901 ccgacgactc tcaattgatt gatctgatct ttttcaagtt cgaaagaatg gttgccggaa
    18961 taaacaaatt gaagtccgat cgcttctcca gtattttctg tcgtatgatg atgaactaaa
    19021 gcaataaagt tattcatatg gtgactgctc gttcctcgac ggctttcaaa tacttttgtt
    19081 ccagataaaa cagattgccg tgaaatctgt ctttcattga catgtgctcc tggcaaagca
    19141 atgacttcat caaatcgcct agtatgggca aagtctagct ggaaactagc cgctttttct
    19201 agtttgattg tttctccggt ttcatttctt atctttacag atcgtgtgat cactgctcta
    19261 tcagtaaata tggtatagaa caaatcgaaa taaatcgctg tttctctatc cttaagtgaa
    19321 atcaccaacg tttgtgcctc tgacttgtcc atcacataag tttgaggcaa gccttcgatc
    19381 tctggcttcc catctgtaat ataatggctg tcatatctca gatccagtaa gtctgatcca
    19441 ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca tgctgccatg agaagagtat
    19501 tcttgcggta gtgtatcttt cgacagagac cggtcaggat ttaagggaac atttcctgaa
    19561 aatccacgat ctcttcgggg ataattttta ttgtctttgt actgcttgac tggtttgcca
    19621 aaataaatat gcgccaatac attgctttct tctatcttta tcagataaga aagaaatgta
    19681 tttcgtaaat ggaagactcg gctcttttca tcaaataaga tgaaattttt atctgttttt
    19741 ctatgcgtaa tatccatcat ttcactccca acaaaacgga aaccgtttac ttttttttat
    19801 aattcctgta tactgtttac taaacaattt tgtcaataaa aattttttat ttttttgagg
    19861 agtgtgtaaa aatggttgga attagagatg tcgcacgaaa ggcaaatgtc tctcctgcta
    19921 cagtttcccg tgtgttgaat aacgatcaga cgatccatgt ggctcagact actcggcaac
    19981 ggatagtaga ggcagctgct gcgttggatt atgacatcag tactcgtaaa tatatcaaaa
    20041 agagaatgcc ttcgattggt gtgatctcta cgatcggcaa agcgagcgaa gaacaggata
    20101 tctattacaa acatttgcga gtagggatgg aagaagaagc tcgacgtctg catttaggga
    20161 tgaatcgaat ctataatcta tctgataatc ctaaacaatg ggaagatcta gatcagttag
    20221 gtgcggtgat cgttgttggg actgttacga aagaatcgat taccgccttg ttaaaacaaa
    20281 acatgcaaat agtcgtcgtg gataaccctg acatccagca agaggtcgat atggtgtatg
    20341 cagatcttga acggatgact aaaaccgtac tggaattgtt cttagaaaat ggtcataaaa
    20401 gaatagccta tatcggggga tataaagtcg atatggatga attcggtggg aagcagattt
    20461 ctataaatga aaagaggatg cgtgcctatc agcattttat gatcgatcat cacttagaaa
    20521 aatatatgtc ttatcatcta ggtggctgga cagaaaaaca aggtgaggta cttgcgacta
    20581 atctattaga aaatgaatcc cctcttccta ctgccattct tgtcgggagc gatccaatgg
    20641 caattggtgt ctatcgggca ttgaaacaaa aaaatatcca aattggagaa gagattgttg
    20701 tagcaagctt tgataatatc gagacaatcg caaacctctc ccctggcttg accagtgttc
    20761 aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat ggcattggag cgaattgacg
    20821 gtatacggga cgaaccaatc attttgactt atcctgcacg attgattgtt agggaaagtt
    20881 ttatcccaag aacaaaaagg tattgaaaga gaggttgtga aaagccgctt cgtttcttca
    20941 tactcaaaac gaaacggctt tttcacaacc tcttacttat acttattcac aaaatccaat
    21001 gtctgctggt aacaattttg cgattctttt agcgtgtaat caaattggta ttcatgggta
    21061 atctcttttt ttgtatctgt ataaaacaag gattgcacgg gtacctttaa ttctgtcaac
    21121 cgattttcta atgcttgacc ttgctcctgg aatgagtatg catttccgtc agttatatac
    21181 gttggcggaa attccttaga aacatggtca gccacactag cttctttcaa ttgctcactc
    21241 gttttccaat cttttgttcc aatcaacgac cacgcaacgg ttttgacgaa aaacttcata
    21301 aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat caacaggtcc gcaataagaa
    21361 atggttcctt tcagactatc ttttggaagt agctgtttga ttttcatttg tttagcatac
    21421 gcatcattcg tttgtatagc tgcatattgc aatgcgattt gtgaacctgc actatctcca
    21481 ccaaaaaata attgttccat gtctagcaat ttgtcgtctt tttcttcagc taataaatct
    21541 ttgatcagtt cattcacttg aagaacttga ttagggtatt ctgaatcagg cgctaattca
    21601 taattcatcg caaccactgc gatgttggcg tctgaaacta attttgtcgc aaattctttg
    21661 acacccgatt tatctccgcc gacgaatcct ccaccatgta cccaaaatag aacaggaaca
    21721 ggccccttgc tgttttttgg atagtagatg tcataagtat tctctttata cttggactca
    21781 tacgtcaaat ctgttttttt cgccacgttt ttttctgctt gatcgaacgt ttttttatca
    21841 gtgatttgaa cagtatttga aaacatatgg ttgatcaaga atgctccagg tctgggggaa
    21901 agctggaagg caagaaagat tgcaaacgcg atcccaagaa tgattccgaa gaaccatccg
    21961 ataaatttaa gaattttctt tttcataagg ctccctcagt agttagtatt aagcattctg
    22021 attataccat agtgttattt cctatcaagc tctcctagat gatttaatga ttttccccaa
    22081 acttcttctg caatgctttt gacaaaagaa attttttcca ttgttcttca aacaaaacat
    22141 tttcttcttc tgttgaagca aatccacatt gaggacttaa gcacaaacga tccaaaggca
    22201 gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg gtgctcttcc atttcgccat
    22261 ttttcgttac agtcaaaatc taagacaact aatttatctt cagagacttt ctctaaaaga
    22321 acaaaactcc cagcacgttc actatcgtat tctaaataat aggcacggac attttcttga
    22381 gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg ccgccaaaat cgaataataa
    22441 tttccgcgtc aaacatgtat atttagtgtt gaatcatttg agaatatgaa aataaaaatc
    22501 tttttgatca atcagttaat caaattgaaa taacgaaaaa tctgagacag aatgacgttc
    22561 attctgaccc agatttgatt ttgcacaact caacagtcct tattggcaaa tcgccaaaat
    22621 aatgtagact aac
//
LOCUS       DANJMI010000044        22464 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000044 DANJMI010000000
VERSION     DANJMI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.044"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            144..542
                     /locus_tag="PWU81_002089"
     CDS             144..542
                     /locus_tag="PWU81_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL1110780.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1220
                     /locus_tag="PWU81_002090"
                     /pseudo
     CDS             526..1220
                     /locus_tag="PWU81_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(1814..2701)
                     /locus_tag="PWU81_002091"
     CDS             complement(1814..2701)
                     /locus_tag="PWU81_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL1110781.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2886..3725
                     /locus_tag="PWU81_002092"
     CDS             2886..3725
                     /locus_tag="PWU81_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL1110782.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3921..4895
                     /gene="sgrA"
                     /locus_tag="PWU81_002093"
     CDS             3921..4895
                     /gene="sgrA"
                     /locus_tag="PWU81_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HDL1110783.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5193..5549
                     /locus_tag="PWU81_002094"
     CDS             5193..5549
                     /locus_tag="PWU81_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110784.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5552..6709
                     /locus_tag="PWU81_002095"
     CDS             5552..6709
                     /locus_tag="PWU81_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1110785.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6735..7175)
                     /locus_tag="PWU81_002096"
     CDS             complement(6735..7175)
                     /locus_tag="PWU81_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL1110786.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7335..7841
                     /locus_tag="PWU81_002097"
     CDS             7335..7841
                     /locus_tag="PWU81_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL1110787.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            8009..9922
                     /locus_tag="PWU81_002098"
     CDS             8009..9922
                     /locus_tag="PWU81_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL1110788.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9927..10928
                     /locus_tag="PWU81_002099"
     CDS             9927..10928
                     /locus_tag="PWU81_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL1110789.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(11130..11336)
                     /locus_tag="PWU81_002100"
     CDS             complement(11130..11336)
                     /locus_tag="PWU81_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HDL1110790.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11982..12830)
                     /locus_tag="PWU81_002101"
     CDS             complement(11982..12830)
                     /locus_tag="PWU81_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL1110791.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12829..13896
                     /gene="gnd"
                     /locus_tag="PWU81_002102"
     CDS             12829..13896
                     /gene="gnd"
                     /locus_tag="PWU81_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HDL1110792.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            13898..14899
                     /locus_tag="PWU81_002103"
     CDS             13898..14899
                     /locus_tag="PWU81_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDL1110793.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14896..15180
                     /locus_tag="PWU81_002104"
     CDS             14896..15180
                     /locus_tag="PWU81_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDL1110794.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15213..16010
                     /locus_tag="PWU81_002105"
     CDS             15213..16010
                     /locus_tag="PWU81_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL1110795.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            16024..16857
                     /locus_tag="PWU81_002106"
     CDS             16024..16857
                     /locus_tag="PWU81_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1110796.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16875..17732
                     /locus_tag="PWU81_002107"
     CDS             16875..17732
                     /locus_tag="PWU81_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL1110797.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17754..18263
                     /locus_tag="PWU81_002108"
     CDS             17754..18263
                     /locus_tag="PWU81_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1110798.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18300..18701
                     /locus_tag="PWU81_002109"
                     /pseudo
     CDS             18300..18701
                     /locus_tag="PWU81_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18813..19166
                     /locus_tag="PWU81_002110"
     CDS             18813..19166
                     /locus_tag="PWU81_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110799.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19200..19643
                     /locus_tag="PWU81_002111"
     CDS             19200..19643
                     /locus_tag="PWU81_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL1110800.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19660..20745
                     /gene="uxuA"
                     /locus_tag="PWU81_002112"
     CDS             19660..20745
                     /gene="uxuA"
                     /locus_tag="PWU81_002112"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL1110801.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20738..21451
                     /locus_tag="PWU81_002113"
     CDS             20738..21451
                     /locus_tag="PWU81_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL1110802.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21658..21807)
                     /locus_tag="PWU81_002114"
     CDS             complement(21658..21807)
                     /locus_tag="PWU81_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110803.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22203..22403
                     /locus_tag="PWU81_002115"
     CDS             22203..22403
                     /locus_tag="PWU81_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL1110804.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7747 a         3450 c         4641 g         6626 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcagcgataa
     1081 cgttaggtaa agagctacta gatcaagtat tagagatgcc ctcgattgat aaagcagtga
     1141 ggggcttcca agaagattgg gtgcaaactt tccaagcaga aacgattggt gaactaaaaa
     1201 aggtcgctga ttacttctga atcaatttct ccataaaata aacgatagcc ttctcgaaaa
     1261 ctcgaggagg ctatctgacg attcaatatt cattaattta aagtttgggt gactattact
     1321 aactattact tatttctcat ttaaacgatg tattccctaa cctctaatac atccttgtaa
     1381 ctctgaagtt acgccagttt tgaataaatt tcataaaaac aaatgaaaag tgtcgcataa
     1441 aaatatcttt cgtgtttctc tagtataaca gaataagaag aacaagtcca gaagggatat
     1501 ttttagtctt ttttaaaata acttttcagt cgagtttgag tttatgatag ttgtttttga
     1561 agcgatacgg caagtgaaaa aatagggaag catgaaagaa tctacttaat ggcgcattga
     1621 ttcatgtgat agaaataaaa aactttttta aattatatcc tcatgattgt aacaaatcca
     1681 gtgatttgtt ttggtacaaa taaaaaaagg ccccgatgaa gttgtggcaa aagtggtttg
     1741 cctcaaaaat aagaaggaat ttccaaaaat cacttctcaa attttgccac atccccaatt
     1801 gggtcaaatt tgtttatttg aatatatttt ttacataagg atctttaata gttacattgt
     1861 cttttttcat cactttacgg attgttttat ccatgaattc cgtatcaaga gtttttgctt
     1921 cggtagcaat tttttctagc tcagatttgt atttgtcttt attagaacct ttatcctgtt
     1981 tttttaccat ttttactaga taatagcttt gctgataagt cgttggatct gttacaggaa
     2041 tgatatcaga aacttccccg ttttttagtt taaatgctgc tttttttact tcagtaggaa
     2101 tctctggatt ggttgaatca aaattcattt ttccatcagt ttcctttaat gttttatttt
     2161 ttccataagc ttgaacaagc ttggagaaat tttctccttc atttgcttct ttttttgctt
     2221 tatctgcttc ttctttagtc gaaaatagaa tgatctgtgt actaacttct ggatagaatt
     2281 ctttccaagc agtgtctaga tctttatctg ttaattttac attggctttc aaaccgtatt
     2341 ggaaagccag catctgttta aagagtttct tatatgtctc ttctgtttgg ccaactgctt
     2401 ttaattgttc cttgtagttt ggaccgagtt ttttgatttg ttcattatat tcttttgtta
     2461 cttcctttga agaaatctct tttccgtata catcatcaaa tgctttgaag acaatcattt
     2521 tttttaatag tgtttgatta tttgatttaa cttttgggtt atcataaaaa tcctgaactg
     2581 taagtgttcc acctttaaac gttgcaatcg tttcattgga cttgctacaa cctgagaata
     2641 gtaaaattgc acatgctgaa aggcacataa ttagtagttg ttttttcaaa aaatttccca
     2701 tcctttcctt attaaaaaca gtctaacaga agaattaggg agacagtctt tcattttcgt
     2761 aaggttgctg tagatattct tagttcgtaa aaatctattc tctctatgat ggagaacatt
     2821 tacatagttt tcacgagaca agaaagaaaa gggttgttaa acttaatata aaggagggat
     2881 gaaagatgat caggattatt cggttgattg gatctgtttt aatagtcttt gttggttttg
     2941 ttttaggaat tcttgtcaat aatgcacttg cggatatgtt tattggaaat agtgaatgga
     3001 ttggagcaat tgccggtacg gtcggacatt tagctgtttt tttactagct ttatttcttg
     3061 ctagtaaggt cgaaggaaaa agaatagaag attatggtgt ttctgttcga ggaaaagact
     3121 gggggtattt agcgggaggt ctcgttgttg gaattggagt gtttctgctt attactagtc
     3181 cattatattt gacgggggcg tatcggttag attcaggaaa cgcaaacatt gtgccgctga
     3241 taacgagttt tattttattt attgcagtgg gggcatcaga agagctttta ttccgtggct
     3301 tttttcagca tcagatgctt agatttggtc cgttaatagc aatgattgga tctgctgcat
     3361 tatttgctct tttgcacgga ttgaatccta acatgacatt tctggcagtt ttcaatatct
     3421 ttttagcagg ttgctttttc agcgcactta tctacagtac aggaagttta tttacggcta
     3481 ttggtgctca tattacttgg aattggacac aaggagctat tttgggtata ccagtcagcg
     3541 gaacgcaaga acaaggtttt ttctccactc tgatccaatc gcaaaataca ataattactg
     3601 ggggaaactt tggggctgaa gcatctatat caactacatt attcttgtta gtcttgattg
     3661 ttggtttgtt actctatgca tataaaacaa agcggatgaa gaaatacact gctgtttatc
     3721 aataaaagat gaagaaagca aggctgatat cggtcttgct tttttaagtc tgtcattgat
     3781 taaacgaaaa agtaaacaaa ttcaagcgaa tacacgacat cttttgttag attttcacaa
     3841 ctgactgtcg tgttctcatc attttcattt tatataattc ctttatcaaa tagaaggtaa
     3901 ttatatggag agtgatagaa atgaagaaaa cagcgaccgt attatttgtt attcttgggc
     3961 tgagtataat tgcagcaatg attggaatca gtacttctgc aaatgaacgg gcaaatgaga
     4021 atctttcatt tactgtgaaa actgatcgga ttgtttatga catgacacaa caagtcatta
     4081 ctattccagt caaaccaaac aaaagcgtga atgctagtga tgtacatgct gttctaacat
     4141 atggttggga tggtaatgga agttcagaaa aagttattgg ggaagtttat ttaaaagacg
     4201 tacaatggac cgctggtatt gagtatacaa taatgatttc tgcagaacta tcgattgatg
     4261 agatcaagag taaagataaa gttgatttga ttgtttttta tgatggacag atgacgatca
     4321 cggagaattt gaaaccaagc agttggacag ttgttggacc gccaagcagt acggatagca
     4381 caagtagtga aagcagtaca gaaaacacga gcggtgaaag cagtacagag agtacgagca
     4441 gtgaaattag cacagaaaac acgagcagcg aaagcagtac ggagagtacg agtagtgaaa
     4501 gtagcacagg aagcacgagc agcgaaagca gtacggagag tacgagtagt gaaagtagca
     4561 caggaagcat gagtagtgag agtagcacag gaagcacgag cagcgaaagc agcacaggaa
     4621 aagtaaataa tgaacttgat ttggtaccta tcataccaac taaggaacaa aagagaaatg
     4681 cagggagcaa cctaaaaagt gtgctaaaac agcctgattt tgtttctcgt ggaattgatc
     4741 atcaagaaaa agcgaaagaa acagataata gaaaaagcga tttaccagaa actggtagta
     4801 gaaccttgaa caagctaatt acatggatgg gtgtgttact cattttgatt gtaggagcaa
     4861 gctattttcg ttctctcttc catcgagtga aataaagaat aaaagcaaca gatctaaaat
     4921 tgaataaaac gatagatttt tgagtgaata tttcattcga gagtgctgta ttgcctggcg
     4981 aagttgtagc tttgccagat ctatttttgt ttactaacat atttgagaaa caggaaataa
     5041 caggaacaca aatggaaaat aagagcaaga ataattgaca atgcacactg cgcatggtag
     5101 tatctctgaa aaatcagtaa cgaatctatt tgtacgttat tcatgtagct gtcggaataa
     5161 gtctaaataa tagataaaaa ggagtatgta ctttgtttga agaatttatt gatatcaatg
     5221 aaaggcaagt atatcaattt ttgaactatt gttatgaaag agacgagaaa ttatatgtgg
     5281 tgaaggatat cgctttagat ctaaactaca ccctggctaa gatgaattcg gtgattcaac
     5341 aagcagaatc gttttgcgaa aggtatccag aatataaatt atcttttctt tcagaaaaca
     5401 agatgataaa agtcgagttt tcttctcagt ttttactgag taaagtttat tctatacttt
     5461 tagagggaac gattggttat attttactgg attcacttta caaaggtact tatcaatcct
     5521 tagaaaatct tagtcaaaaa attatataag tttgcgtacg attcaaagaa aattaagaga
     5581 acttaatact attttgaaaa actatcattt atcttgcagc ttgaaaaaag gtaatccgat
     5641 agaaggagaa gaataccgta tccgatactt ttttcacttg atgtattggc agtcattcga
     5701 cgaaaatcat tctgatatat tatcaagtat aaaaaataaa cgtcaactat tagaaaaatt
     5761 tgcgaaacat gcgccatata cgcgtagtat agatagaaag aaatggatca gcctactaag
     5821 aatttcgttg caacgaatga aacaaggcca ctcgattacg tatgtcccag aagagatgaa
     5881 caaatttgtg catccattga taacaaaaga agagtttacg cattacatac tagagccttt
     5941 ttttgaagca aacttctggt cgatgaatca catcactcca acagagcaag cttaccttta
     6001 ttttatgttt ggtgtaatga acacctattt ggaagaagat ttgttggata ctccagataa
     6061 agtacaagaa agttggggct ggattgaatc gtctattgta gaaaattttg aaaaaaactt
     6121 caagatttgt tttacaactt cagaaaaaaa ttatctatat gtcaatttgg caatgatcca
     6181 tttgtattca agggtttttg ggacaaaaaa gaaggtggat gcttttggaa aatcagcaga
     6241 agacagagat tttcagcata tatttccagt gatgtatcca atcatgcaac agttttttag
     6301 tggggtagct cgcagaattc cagagcttaa tacttattat gaaaagaaca gacggttgat
     6361 ttttcaatac tgtatgttag tcagggatat ttttataaaa cacgaacaac ctgtcatatt
     6421 ctatattcaa tcaaagtatg gtaaaacaca ggaattatgg gcaaagaaaa ggatattatc
     6481 tatgactgaa cgcccaataa aatatagtgc atccgctgat cagttacctg atattgtcat
     6541 tagcgattat cctatcgaca aaaaggcata tgatcaaacg aatacacaga tattttactg
     6601 gaatggacaa cctacaaaaa atgattgggc gcgtctgaaa gctgaaatca taaaaatcag
     6661 gaatgagaaa aatagatcca tattggaaaa attggatttc gttaattaag aaaagcagat
     6721 tgtttatgcc aaaattaaga cattgcaaaa agattatttt ctttaaaatc gacaagatga
     6781 ccagaaagca agagttcgtt gagctcttgc tttcttttag ttttagaaag gaacatcctg
     6841 attttatctt cgctaaatcc agtcaaaagt tttgcagatt ggtcgtagat aatcggtttt
     6901 ttcaataaag aaggggatgt acgaatctca atatatagtt cactcaaact gagttcatct
     6961 attctgtgaa caagattttt ataactaata gaatgagtag agagcatttc ggtcacgccg
     7021 ttgtttgtca attttagtaa gtggataaat tcatttttgg ttagtggttg acgaattaag
     7081 ttctgttcga taaacggtat atcatggacg ataagccaac gttttgcgcg tcttgaagac
     7141 gagcagccag aacctgtata caatttaatc atcatacatt cctccaatag ctaagtattt
     7201 atgaaataat aacatgaaga aatagtcgaa aagtgccatt tagaggtgta aaataagaaa
     7261 atgttgtcac gtataatgga actagctaga taagaaatac tattaaatca agaaaaaaga
     7321 aagaaggcac aagtatgtca aacacagccg acgcaatagt agtgatcgat ttacaaaaag
     7381 gggtatgttt tgattcttcg ccgatacacg aattagaaac agtgacagca ttagtgaata
     7441 aaagaatcaa aacatacgaa acgattggga aaccagttat tttcgttcaa cacgaagatg
     7501 ctcaattagt gccacaaaca aaaccatggg agctattgtc ggaattatct attcctgagc
     7561 acagttattt tgtgagaaaa acccatgcga attcatttta taagactaat ttgagtgttt
     7621 tattaactga attggactgt cattcgatcg aattatgtgg ggcacaagca gagtattgta
     7681 tagatacgac tgtgaagttt gctcatggat taggatacca gcttatgatg tgtagaggag
     7741 caacgacaac ttatgataat ccttttatga aagcaaagga aacaatcgct ttttacgaga
     7801 aaatttggga taaacgattt ctgacatttt tggagcaata agttgtttct tcgtttaaaa
     7861 aaagcagaat ggatactggt ttatccattc tgctttatta cgtaaaatga gaaaataatc
     7921 ctgttctatt tttatgggat aaaccattta ttttttgtat aaaaaatgtt tgcgtttaca
     7981 tataattctc ctagaaacat aaaaaggagt gaatagcttg agaacaaaac aagtattaaa
     8041 gggagcgttg gtcatcttat ttgctgtaag tacaagcgtc ccgcaatgga cagtatatgg
     8101 agagagcatt tcttccgaat cggaatctcg ggaggtagaa accacagaaa ctactgatgc
     8161 tggaaatgaa acgaaatcga gtacttcaca aacagaaaca aatactgaaa caacggttga
     8221 gacatctgtt actagcacag atcaagaaac gaccgctacc acagataacc aacaaacaga
     8281 agaagcaaca gctacaaaaa tgtcaaacga cgaatcctct tctgctaaag taagtgacaa
     8341 agagaatggg acagaaaatg atgatatcgg taaaggtgaa aatttcaatg gtaaagaata
     8401 tttgaattac ttcggtaaac agatgacaga aatacttaat ggaccaacag aagacatcaa
     8461 gtcaaaatta atagagttat tagtcactga gcttgctgtt aatgattatg gtgtagataa
     8521 agagattatc aaacatctga ttgctactta tctattgtac gatatcgctt ttgcaaaaaa
     8581 cagtgtgttg atgtcgatgc ttggaggtac agatgatggg acaacgccta cagaaaacac
     8641 aggcttctat ccaaatacaa aacaacttca tcaaactgta ctaaacaaac aagatggcaa
     8701 aatgatggat gtatatgcat attatgtcga tcaaggttca gataaaacag tcatgatcca
     8761 tggcggattt cgagggaatt ggaataacgg gatcgttaca gaagaataca atgattttta
     8821 taaggccggg tataatttat tatttgttga ttccagagcg actggtaata gcggaggcga
     8881 ttatgtgacc tatggacaat atgagtcaga tgacgtcctt tactggataa atcaagaagt
     8941 tcgagagcgg ccatctcaaa agattttact ttatggtggt tcgatgggag cagctacgat
     9001 gatgtctgta ttagcgaaag atattcctgt caatgtcaaa ggaattattg aaaattgcgg
     9061 atttgcatca attgatgaac aactacgttt tacgtacagc cagacagtag cccccgcact
     9121 accggatgcc ataaaaaatc agttagacat cattggggat caagagcatg aagatttatt
     9181 tatgggcctt ttgaaacaat actattttga tcaggagatg catttggata caaaagctgc
     9241 tctacctacg attggaatgt cagattcatt gcctaaattg attatccatg gaacagcaga
     9301 tgatgtagta cctgtgtcta acgcgcaaaa attatatgag ttatcaggag ggtataaaga
     9361 tcttttatta gtagaaggag cagggcatgg aaaggcacag gaagttgacc atgctgccta
     9421 cacgaaacat gtaacggact ttttgaaagt agtgttccat gatcagataa acgtaaagta
     9481 tgtggatgaa aacaataaaa gtctactcaa agaccaggac gaaatccagc tttacggagc
     9541 ttatggagaa aattatatga cagagcaaaa gacattcgaa ggatacgaat tagcaaatgt
     9601 tgaaggaccg actgaaggga tttttaatga gacgatacca actattattt tctattacaa
     9661 aaaaatccca gtagctccac caaaaaagca agatccgaca gagaatgcag acaataaggg
     9721 aaagaatgaa cctcgagagg atcaatcaag tgatgatacg caaccgattc agatgacagt
     9781 gaaagtacaa aaaaagacag atgcaaaaaa aagaatgttg ccgaaaactg gagaaaagaa
     9841 acattcgctt ctcatttatt caggtggatt tttatctgtt agcacgatta tcacatggat
     9901 ttggcgaaga aagagagggt aaatgaatga aatggttcaa gcgaatagtg attgcagtac
     9961 cagttatcat cgtagttatt ttagtcactg tatttttgat taatcaaatc acaccaaagc
    10021 cgattagctt gcttgttaga aaacaatttg acacaagtga aaaagaacaa gtatatgccc
    10081 gaccagatga tttccagcaa aaaatcgaac aaatcgatat aaaaaaagaa atatcctatc
    10141 cttcaaagta caaaaataat aatatggata tctacacacc gaaagtggaa aagaaaaaat
    10201 taccgattct tttctggatg catggaggcg catatgtagg aggggataaa aatgattgtc
    10261 gtgattattt ggaatatcta tgttcagaca cgcagcaaat catcgtaaat atagattatg
    10321 aacgatcacc ggaagcaaaa catcctacac cagttatcca attgaacgaa gccattcaag
    10381 cagctaaaaa agagataaaa gatcaagcag attggtcgaa tatttcgatt ggtggaaatt
    10441 cggcaggtgc acaaattagt ggagaatatt tgttagcact tcaaaatgaa gaaattaaaa
    10501 aagctgatca tcttgatccg actttggaga acaagcagat tacgaagttt atttctttgt
    10561 ctggtctgtt ggatccttct gcacttactc aagtaagtga taaaatctct agctttcttt
    10621 atgaaaaatg tggatgggct tactttgata ataaggactt tgaacaatca gagaaggtca
    10681 aaagtttagc cttggtcaga catgcggatc gttggacgca aaacacattc ttaacggatg
    10741 gaaatacaaa tacgtttacg aaacaaatgg aagaagtaac ttctgtattt gagaaagcgg
    10801 gggtgaaagt cacaaaagta gattatgcgg agaaaaatgc tgttttgaac catgaatatc
    10861 agtttgattt cagcaataaa caagcgcaag aaacatatca aaagttagta atgtttttca
    10921 aggagtaaag tcttatgatg caaaagaggt tgtaatctgt cacttagctc caagtcacaa
    10981 ggagcaattt caataaacaa gcttttcatg gtttatgaat attgtaactt gtgactgagg
    11041 agttagagat ggcaaggggc accttttatt cgaaatttgg gaatatcaca cgattattgt
    11101 cacgatctct tttacgtaaa ttttataagt cagatttgtt gttttgatcg tttaaaaaaa
    11161 gcaacaaatc cgaagcctgc ggaggcaaga agggacaaaa agccgagtgg taagcaaaag
    11221 aatgctggtt ccactaatat tccgttgtta ttgatataag gctgggaaca aagatgaata
    11281 gcaaataaaa tcaatgcact agcgaaaaaa agcaaagaaa tatggatagc tttcataagc
    11341 agcacctcct tttttataat tgtatgataa atagattaag aatctataaa tgtgacaagt
    11401 ttgttaggat gtgaataaaa tgatcatcta gcgaacttga aacaaagatg gctaatgata
    11461 agcaattaat gattcagcaa tcagataggt acaaagttta ccatcatacg tctagaccaa
    11521 taatagaaaa tatgggaata taatagaaaa gatttaggat acccccaaaa gaatttcccc
    11581 aaaaattcga tgtatcctaa acttttttat tttacataac aatcatatca actgtgaaat
    11641 aaaattacaa atgataacct aaaatgaggc caaaagccag tgttatcaag gaaatactta
    11701 attcaccaaa atttttttta attttttaga aaataagaaa caaaaataat aaaaaaaaga
    11761 aaagataaat tttttactac tgtatactaa ctttgttatc gcatctaaca agtaactcga
    11821 ataataccaa tacaaccttc tccaaagatg cgatgacata cgttctaacc caccgactat
    11881 tgatgttctg taccaccctt gcagaaccaa aaaagccact accaaatatt tggtagtggc
    11941 tttttttgtt ttatggattt ttcgcttttt ctgttatggt gctattcttt gatttcagaa
    12001 atagtatgtc gaactttagc gatagaacga cgtgattctt tttcgttgta aaacatcaaa
    12061 atcacatata gagagtccaa aatgcttagt tgagaaatac gggaagctaa agcttcagat
    12121 cggaattctg tttcttcaga aatagaaata aaaacgacat cgccaagttt agcaagggaa
    12181 gaattagctt ggctagtaat aacgattgtt tttgctccag tttttcttac ggtttcagca
    12241 atatggattg tttcttttga ttgccctgtg tgagaaatca aaacagcaca gtctttttcc
    12301 gtcaataaag aagcttccat caattgaata tggtaatccg tgctgtgaga agttgcgata
    12361 ggggaacgca aaaatttatg ataagcgtca gttgctacaa tctctgaacc gccaacaccg
    12421 aaaaaataga cacgtttaga ttcattgata atagtagccg ctttttttag atccttttgt
    12481 tgtaacaact tttttgtgtc atttaaagtt gaaatactag actcgaacac cttttgcgcc
    12541 atagtgagct cagtatctgt tttctgaata ttttcgtgga tagaaatagc agatagatca
    12601 ttttcttgga tcaacatggc cattttaaaa tctttaaatc cattataacc aagttttttt
    12661 gtaaattgaa aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt gctaatagga
    12721 ctatgactta ctttgcttgg attttctaaa atataatcgg cgatagcctg ttcttttggg
    12781 ctgaagtcgt tgtacattgt tttgatttgt agtcttatgg agacgctcat gagaattcct
    12841 cattttcata ataagattat cattttttcc gttctcatta atgttaaaga tacaaggaga
    12901 gtaggaaaaa atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa aatttttttg
    12961 tataatgagg gtataagaaa tggaggcgct atcatggaaa ttggtgtaat tggtttggga
    13021 aaaatgggat tgaatttagc tttaaactta aaagagcacc atcacaaagt acaaggactc
    13081 gacatttcaa gtacagctgt tagtcaggca agagaaaatg acattcctgc ttatcaagag
    13141 atctctgaat ttctagctac ttttgagcag aaaaaaatca tctggttaat gcttcctgct
    13201 ggagagataa ccgaaaacat gttaagaaaa ctttttccaa aactcggacc aggagacatt
    13261 attattgaag gcggaaactc ttattataaa gattcaatcc gtcgagcaga agagtttcag
    13321 aaaaatgaaa tcggttactt ggattgtggg acttctggag gaatcgaagg agcacgacat
    13381 caagcctgct taatgattgg aggagataaa gaaacttttc tatccgtgga acaattattc
    13441 aaagatgtag caattgaaag aggatatctg tatgctggtg catccggaag cggacacttc
    13501 ttgaaaatga tccataatgg aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc
    13561 caacttgtac aaaaaagtga gtatgctttt gacttggaac aagtcgcacg tgtttggaat
    13621 catggttcgg tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt aaacgaacat
    13681 cccaatttaa gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt
    13741 gaaacagcgc tagaaatggc tgttccagtt ccagtaattg caactagttt gttcatgagg
    13801 aatctttcac aggaagaaga tagtttttca gcaaaagtag tttcagcact aagaaatggg
    13861 tttggcggac atgaaatagt tgaaaagggt gagtaaaatg ccagattatc atatagcaat
    13921 cgttaattcc agcagtttcg gaagagtctt tccacaccac ttggaaagat taaagtcagt
    13981 tggagacgtt catttcttta ctgttgatca agagattcca ggaaaagaac tagcggagtt
    14041 acttaaagga ttcaatatca ttattgcaag tgttacgcca ttttttcgac gagatttttt
    14101 tgaaaacaaa gatgaactgc tattgatttc ccgacatggt attggttaca acaatattga
    14161 tttaaaagca gcaaaagaac accagacaat cgtatctatc atacctgctt taattgaacg
    14221 aaatgctgta gcagaaaata atataactaa tttgttgtcc atgcttcgac gcacgacaca
    14281 agcacaagca agagtcaagg acgatcgatg ggaagatcgg gcggagttcg tcggtaggac
    14341 gctgtttaac aaaacagttg gtgtgattgg tgcaggaaat accggaagcg gagtaatcga
    14401 aattttgcgt aacggttttc gctgtgatgt tctagcttat gatccttaca aatcagattt
    14461 atacatcgaa agctttggtg caaaaaaagt agaacttcaa gaattactgg aacaatcaga
    14521 tatcatttgt ttatgtgcga atttgacaga agagaactat cacatgattt ctgataaaga
    14581 agtcgatcag atgaaagaaa atgtttatct atctaatagt gcaagaggcg ctctagttga
    14641 tgaagaagcg atgattaatg gtctgaaaag cggaaagatc gcaggatatg ccacagatgt
    14701 tttagaagaa gaaccgggac gtcaagatca tccgtattta gcgtttgaac aagtagtgat
    14761 tacgccacat atatcggcct acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt
    14821 cagagatgtc gaacagatca gtcagcacct tcttcctgaa cgttctgtcc agacaaagag
    14881 caactatata aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct
    14941 caattttatt tatatgaaga aggtggtatt tcaaaagttt ctgatctctt gaaatcatac
    15001 ggcgcgaaaa gagtactggt cacccacgga acggtatcat gggaaaaggc gctacctaaa
    15061 ttagtatttt tgaatgacga aacgattcaa tttttttatc atcgttattc tggagaatgt
    15121 agttatgctg aagccagacg aatagctaca atcatcaaaa aaatgaaatc gatttcgtag
    15181 ttggcgttgg cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa
    15241 tcgatttcgg agtcatccct actttagcta gtaattgtgc tccatggaca cctcttgctg
    15301 tcatgtataa ggagaatgga caagcagaag ggaaaacaga acacttttac agacaagctg
    15361 ctttttttat aacagatccc caattagtga ttgattcccc tgttccatat ttcattgccg
    15421 ggttagcaga tacactagca aaatggtatg aatctgagct catcttagaa caagattttt
    15481 tgcaagatga atcatttttg aagctagcac aagatactgc gaagatctgc aaagaagaga
    15541 ttttgaatca ttctgctaaa gcaatccaag atatggcaca aagaaaactg acatcggagt
    15601 tcaaacagct ttcagagatt atctttgctg ttgctggttt agttggagga ttaggagata
    15661 aatacgcaag aaatgcggca gcacatgcta tgcatgacgc tatcagtaaa tatattccgg
    15721 aaagccatcg ttttctacat ggagaaaaag tagcgtatgg gattttctat caactggcac
    15781 tagaaggtcg ctgggaaaaa atagacgagt tatatccttt gtatcgggaa ttagaactgc
    15841 cggtttctct agaacaaatg ggattatttc cgaaagataa acagataata gataaaatgg
    15901 ctgaatttat tgattcgaaa gagaaagtgc atttgattcc tatagaggta acacagaaat
    15961 cactgataca gtcaatgatg gaactggaag gttatatcag aaagatctga aggaggagga
    16021 cacatggaaa cattaagtgt agtacaaagt ttgttgattg ctttatgggt agccgcggtt
    16081 atgtcccgct ggttaggtgg aggagccacg ttgaccttac gtttttctcc tttgatgact
    16141 ggattagtcg tcgggttgat catgggggaa gttgcacaag cgatgattgt tacagccgct
    16201 ttgcaaatga tttatatggg ggttttttct ccaggaggat caatgccagc tgaaccatct
    16261 attgcagcag ctatcgcagt accagtcgca ttattagggg atctgtcgcc agaagcagcg
    16321 attgccgttg cggtgccagt cggattgctc ggaagttact tgtatcagtt tcgttttttc
    16381 atcaatactt ttttaggtaa atatacagac aaagctgtag aagatttgaa tacaaaagct
    16441 atggcccgat caattattct ttatccaacg gttgcctcat ttttattatt cgttccgctt
    16501 gttttcattg ctttatattt aggggcgcca ttgatcgctg atgtaatcaa agctttagag
    16561 ggaacagtcg ttatccacat tcttgaagta gtaggtggag ggttagcggc aatcggtatc
    16621 gcgacaactg tatacgttat cggtcgaaaa gattatctgg tctttttctt ccttgcttat
    16681 tttatgagtg ttgttttgaa atctttagat attacgatgg ttacttatgc catttttggg
    16741 attattatcg ctttgatctt tgtccagtcg caaaaaggga aacaagtaca gcctgccaca
    16801 agcaataaca attcagcatc ttttgatgac gaagatgact atgatgacgg attttaattt
    16861 aggaggaatg ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca
    16921 cgaaaaaaga tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact
    16981 cttttgaacg ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac
    17041 tttataaaga tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca
    17101 ctcaattaag ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa
    17161 gagcagaaga agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca
    17221 cgaaagcggg attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg
    17281 tccaatatat ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg
    17341 cactttttcc ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa
    17401 gacaagcttt cactatgggc cgtaatgcta caggcctaat gcaaaacact ggtgttcaga
    17461 cagcgattga aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact
    17521 atgtaaaagt agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg
    17581 aaattcttga tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact
    17641 tgttctatac gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat
    17701 taattgtgct agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg
    17761 tagtcaattt agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat
    17821 ctcaaaaaag tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat
    17881 ttatgagaga tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta
    17941 caccagagaa agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt
    18001 tgatcgcaaa aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag
    18061 aattgaatat tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat
    18121 ctgtttattt aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg
    18181 taaagaatat ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat
    18241 tgaacaagtt tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa
    18301 tgaagaagaa cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc
    18361 gccgtacaaa aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta
    18421 tgagaacaga tgtgacagtc attgcttacg atacgctagc ctaccgataa aaaaatattt
    18481 atgttggaga tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc
    18541 atgcattagg aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact
    18601 ctatattatt gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata
    18661 acaaaaatcc tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag
    18721 catttgggag ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa
    18781 cttttattcg aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg
    18841 atccgagtgt agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca
    18901 gcgtgagatc gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag
    18961 atacaccgct acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag
    19021 ttgataagtt gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc
    19081 agttcttcta tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca
    19141 atcagattat tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa
    19201 tgttggaaat catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg
    19261 aagtaattgt cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca
    19321 tccaaacatt cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca
    19381 tgctagtatt tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca
    19441 atgaactgac tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa
    19501 tgttgttaga agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac
    19561 aaattgtcaa acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa
    19621 aattagatga ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg
    19681 tatggtgaaa aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat
    19741 gggataattg gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt
    19801 gaggagttga aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta
    19861 gcgatccacg atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat
    19921 atacgaacaa tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa
    19981 ccaatttttg gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct
    20041 cttttatacg atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata
    20101 cacagccagt caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc
    20161 gctcagctgg aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact
    20221 tattttttag aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat
    20281 ccagatgatc cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac
    20341 ttgaagaaaa ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga
    20401 tctttagggg caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc
    20461 atccattttg tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg
    20521 gcacatccct ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa
    20581 gaagggttcg acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct
    20641 atgcctggat atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat
    20701 gaggcgataa gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat
    20761 ctttgatatg gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa
    20821 atcagcacaa aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca
    20881 aacagaaatg gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat
    20941 caaatggcga gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt
    21001 atatacgaaa aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac
    21061 aatcgtggct tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct
    21121 atatgccttg tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc
    21181 agagatcttt ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt
    21241 tgaagactcg attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat
    21301 caaagatcat ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt
    21361 aagtttatat gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg
    21421 atttttagaa agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta
    21481 tttattgtcc tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt
    21541 atctacctta tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta
    21601 ccagtagatg cgtttaaaga gaagaacgag aatcgtccat atgagggttt ccactcctta
    21661 tgcagatttt tttaattctt gcactgcttg ttcgatcgta acacctgtcg ctacatgatt
    21721 ggtattttct ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg ttactcgata
    21781 ttgtatatct ttgttataga atgtcatagc cattctcctt tctttaaata cttgctgaaa
    21841 ggcaacgaac gcgttgataa cgaaattcca tttataagta taccaccaat tgaaaggttt
    21901 tcatagttaa gaaattttaa agtcaaaagt tgcagataaa agcaaataaa caagaaaaca
    21961 aaaagtgaat caatcgcggt tattcaactt tttcttcgat tcgaagacaa tagaagacaa
    22021 tgaattttat atttcattgc attatccttg tgttattaga aaaagcatga tatattaaat
    22081 gagtaatcta ttttgaaacg taatttgagc gatatatcta catagataaa actccttttg
    22141 cctatcaaat atttattgca acgaaaaaac gtacattgat acgtatcagg aggaatttat
    22201 ttatgaataa cggtacagta aaatggttca acgcagacaa aggttttggc tttatcactg
    22261 gtgaagatgg aaatgatgta tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa
    22321 ctttagaaga aggccaagca gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag
    22381 caacaaacat tgttaaagct taataaaaat tgacttttaa ccgtcttttc gaaagaaaag
    22441 acggtttttt tgtacgaaaa aatt
//
LOCUS       DANJMI010000045        22423 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000045 DANJMI010000000
VERSION     DANJMI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.045"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            6..305
                     /locus_tag="PWU81_002116"
     CDS             6..305
                     /locus_tag="PWU81_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1110805.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="PWU81_002117"
     CDS             378..1739
                     /locus_tag="PWU81_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDL1110806.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="PWU81_002118"
     CDS             1761..2021
                     /locus_tag="PWU81_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110807.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="PWU81_002119"
     CDS             2035..2787
                     /locus_tag="PWU81_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDL1110808.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGKKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="PWU81_002120"
     CDS             complement(2881..3486)
                     /locus_tag="PWU81_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL1110809.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="PWU81_002121"
     CDS             complement(3539..4513)
                     /locus_tag="PWU81_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDL1110810.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="PWU81_002122"
     CDS             complement(4891..5610)
                     /locus_tag="PWU81_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1110811.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PWU81_002123"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="PWU81_002123"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL1110812.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="PWU81_002124"
     CDS             7161..8558
                     /locus_tag="PWU81_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL1110813.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="PWU81_002125"
     CDS             8639..9079
                     /locus_tag="PWU81_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL1110814.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="PWU81_002126"
     CDS             9227..9553
                     /locus_tag="PWU81_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL1110815.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="PWU81_002127"
     CDS             9554..10303
                     /locus_tag="PWU81_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110816.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="PWU81_002128"
     CDS             complement(10383..10838)
                     /locus_tag="PWU81_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL1110817.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="PWU81_002129"
     CDS             complement(11265..11540)
                     /locus_tag="PWU81_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110818.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="PWU81_002130"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="PWU81_002130"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDL1110819.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="PWU81_002131"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="PWU81_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL1110820.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="PWU81_002132"
     CDS             14770..17436
                     /locus_tag="PWU81_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL1110821.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="PWU81_002133"
     CDS             17800..18552
                     /locus_tag="PWU81_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL1110822.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="PWU81_002134"
     CDS             complement(18574..19728)
                     /locus_tag="PWU81_002134"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDL1110823.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="PWU81_002135"
     CDS             19913..22354
                     /locus_tag="PWU81_002135"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDL1110824.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         7199 a         3933 c         4672 g         6619 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtaaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagctg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt ttt
//
LOCUS       DANJMI010000046        22175 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000046 DANJMI010000000
VERSION     DANJMI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.046"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            240..2075
                     /gene="tdc"
                     /locus_tag="PWU81_002136"
     CDS             240..2075
                     /gene="tdc"
                     /locus_tag="PWU81_002136"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL1110825.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2380..3858
                     /locus_tag="PWU81_002137"
     CDS             2380..3858
                     /locus_tag="PWU81_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1110826.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3824..5155
                     /locus_tag="PWU81_002138"
     CDS             3824..5155
                     /locus_tag="PWU81_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1110827.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5269..7821
                     /locus_tag="PWU81_002139"
     CDS             5269..7821
                     /locus_tag="PWU81_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDL1110828.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7828..9267)
                     /locus_tag="PWU81_002140"
     CDS             complement(7828..9267)
                     /locus_tag="PWU81_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1110829.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9613..9975
                     /locus_tag="PWU81_002141"
     CDS             9613..9975
                     /locus_tag="PWU81_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDL1110830.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10162..11019)
                     /locus_tag="PWU81_002142"
     CDS             complement(10162..11019)
                     /locus_tag="PWU81_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL1110831.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11139..12050
                     /locus_tag="PWU81_002143"
     CDS             11139..12050
                     /locus_tag="PWU81_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL1110832.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12057..13708
                     /gene="mdcA"
                     /locus_tag="PWU81_002144"
                     /pseudo
     CDS             12057..13708
                     /gene="mdcA"
                     /locus_tag="PWU81_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            13810..14109
                     /locus_tag="PWU81_002145"
     CDS             13810..14109
                     /locus_tag="PWU81_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HDL1110833.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14099..15775
                     /gene="mdcD"
                     /locus_tag="PWU81_002146"
     CDS             14099..15775
                     /gene="mdcD"
                     /locus_tag="PWU81_002146"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HDL1110834.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15779..16372
                     /locus_tag="PWU81_002147"
     CDS             15779..16372
                     /locus_tag="PWU81_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HDL1110835.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16396..17472
                     /locus_tag="PWU81_002148"
     CDS             16396..17472
                     /locus_tag="PWU81_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDL1110836.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17497..18366
                     /gene="mdcB"
                     /locus_tag="PWU81_002149"
     CDS             17497..18366
                     /gene="mdcB"
                     /locus_tag="PWU81_002149"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HDL1110837.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18477..19241
                     /locus_tag="PWU81_002150"
     CDS             18477..19241
                     /locus_tag="PWU81_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HDL1110838.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19730..21823
                     /locus_tag="PWU81_002151"
     CDS             19730..21823
                     /locus_tag="PWU81_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL1110839.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21848..22150
                     /locus_tag="PWU81_002152"
     CDS             21848..22150
                     /locus_tag="PWU81_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1110840.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7179 a         3788 c         4725 g         6483 t
ORIGIN      
        1 tgtttgttcg ctttttttag gttatctatt ttagaagact gatttattga aaaattgttc
       61 gcttttttgc gttgaattat cagaaaaatc cgactttatt tatttttgtt tgtaaaaatc
      121 tacgtggatt tgatgttata aaaaaaaaat tctcattttt ggcgctatag tgaagaggtg
      181 ttacagataa acacatgtat ctgcaagaca ttgaccaaca aaatatggag ggaagaaaaa
      241 tgaaagatat ggatatcaag gccgtcttta taggcgacaa agctgaaaat ggaccagttt
      301 ataaaatgtt gttgaacaag atggtagatg aacatcttgg atggagagaa aattatattc
      361 cttcagatat gcctgcaatt agcgaaggag ataagctcac tccagattat ctagccaccc
      421 gcgaccatat gatagaggtc ttggatgaag tcagtcagcg attacgcgca ggatcgatcc
      481 catggcattc agcaggccgt tactggggac aaatgaatgc tgaaacattg atgcctgctt
      541 tattagctta caactatgcg atgctgtgga acccaaataa tgttgcattg gaatcttcta
      601 tggcaacttc gcaaatggaa gcagaagtag gacaggattt tgcttcatta ttcaatatga
      661 cagacggatg gggacacatt gcagcagatg gttctattgc taatcttgaa ggattatggt
      721 acgcccgttg catcaaatcg attcctttag ctgtcaagga agtgctgcct gaaaaagtga
      781 aaaaaatgtc tgagtgggaa cttttgaatt tatctgttga agaaattttg gaaatgaccg
      841 aaagctttac ggacgaagaa atggatgagg tcaaagcagc ttcttcccgt agcggaaaaa
      901 atatccaaag attagggaaa tggctggtcc ctcaaacaaa acactattct tggatgaagg
      961 cgttagatat ttgcggagtc ggcttagatc agatggttgc tatcccagtt caagaggatt
     1021 atcgaatgga tattaatgct ttggaaaaaa caattcgtga attagctgga caaaaaatcc
     1081 caattctagg tgtggttgct gtcgtaggaa cgacagaaga aggtcaagta gacagtgtag
     1141 ataaaatcgt ccaattaaga gaaaggttaa aagatgaagg gatctatttc tatttacatg
     1201 ttgatgccgc atatggcggc tatgcgcgtt cactgtttct aaacgaagca ggagaatttg
     1261 tgccgtatgc ttccttagct gaattctttg aagaacatca tgttttccac cactgcgtaa
     1321 cgattgacaa agaggtatat gaagggttca gagctatttc agaagcggat tctgtcacga
     1381 tagatcctca taagatgggc tatgtccctt atgctgctgg aggaatcgta atcaaacata
     1441 aaaatatgag aaatatcatt tcttatttcg cgccatatgt gtttgaaaaa tcagtaaagg
     1501 ctccagatat gttaggagct tatattttgg aaggatcaaa agctggagca accgccgccg
     1561 cagtctggac agcacatcgt gtattgccat tgaatgtgac cggatatggt caattgattg
     1621 gggcttcaat cgaggcagct caaagattca gagaatttct cgatcatctg acttttactg
     1681 ttaaaggaaa aacaatcgaa gtctatccat tgaatcatcc tgattttaat atggtcaatt
     1741 gggtattcaa agaacaaggc tgtacagatt tgaacgctat caatgaatta aatgaaaaaa
     1801 tgttcgatcg gtcttcttat atggatggcg atgtttatgg tgaacggttt attacttctc
     1861 atactacatt tacacaagaa gattacggtg attctccgat tcgttttgtt gaaagaatgg
     1921 gtctaacgaa agaagaatgg aaaaaagaac agaaaatcac tctgctacgt gcggctatca
     1981 tgactcctta tttgaatgat gatcgaattt tcaattttta tacaaaaaaa atcgccaaag
     2041 caatggaaaa gaaactaaac gaaatcatcc aatagaactg atttttgtgt gaaagaataa
     2101 caagagacat atggagaaca gacaattatt tgcttcatca aaacaattgt atttccccct
     2161 gtttttcata tattcctcgc caagatattc ttttttataa aacgttctat aagttggatc
     2221 gttcgtttca tgtatcaaac ccagttcact aagatcgttc aatggatgtt tcggtgatac
     2281 tgggttttgt actgaatcga tgaaaccttg tcatttctgt tcttgaacaa aaataaaaga
     2341 aaacaaaaac atatgtcgct attgaaagaa ggaataaaga tgaaagatac tggttcatca
     2401 aagcaaatca cgaccatgac ctttatcgga atgacctgcg cccttgtagc aagtgtacga
     2461 aatattccag atgtagcagc aacgagctgg acgatgattt tttacatgct ggttgcagta
     2521 ttattgtacg cttttccaat ctcattgatc tctggtgaat ttgctggaat gtttccccaa
     2581 aaaggtggtc cagaactttg ggtatcgaat gctctaggaa aaaaatgggg gttcgttgtc
     2641 tcatggctgt tatgggtcca aatgtttcct gggatggtga tggtcgcttc agcactcgct
     2701 ccgttgtttg gtaatattat cgataatgtg ccgcttggtt tgaacagcaa atttacatta
     2761 gttgtcattt tagtcgttta ttggatcatc acttttttga atttgaaatt tgatatggca
     2821 aaaatcggcg gaaaagtcgg tgtttggtta ggattgtacg tgcctttgac gatgatgctc
     2881 ttattagggt ttgctgcgtg gatcaaagtt gggatcgtac caaccggaac attaggcagt
     2941 ttttcatggg acaaattaat accagatact acgactgcat cctcatttgt ttattttgcg
     3001 ccgatcatgt ttatttttac tggtatcgaa atgtcctctg tgtatatcac tcgattggaa
     3061 aatccagtta aaacgtatat tcgcggagta tttgccgcgt taatttttat atttttcgtc
     3121 aatattttga atgctttagt tgtagcaaat gtcgtaccaa aaggacaaat ggaattgaat
     3181 aatatcgcgc aatctatttc gatttattgc caaattcttg gtttgccaca tcttattgtg
     3241 aatctgttta gtttacttgt cttcattggt gtagcagtcc aattatctgc ttgggcctca
     3301 ggaccagcaa agacagtgac agaaagtgct agaaaaggtg cgtatccacc aaaattcaat
     3361 ttttggaaga cgaatcaatt tgacgtatcc aaatccgtta ttttgactca gtctgtcatt
     3421 atctcaatct ttgcactttt ttatttgctg attcctggcg tcaatcaagc atttttgatg
     3481 ctcgtaaatt caactactgt tatctactgt atcgtctatg tcatcatggg gatcgcagtt
     3541 ttgagattgc gttatacaca tgcaaagttg aatcgaccat ttagaattgg aaaaaaagag
     3601 aaaaagagca atcttggagt ttggactgtc gttattgttt tatttgcggc aatcggattt
     3661 tctgtcggac tgacgatgaa agctggaaca tggatcaatc taattgcagt cacagccatc
     3721 agtgtcattt tgtttgtagt accactctgt attgaaaaaa taaagaaacc ttcttgggaa
     3781 caggaagtcg ccatgctgct tgaaaaagaa aaaggtgaac ttgatggaaa aaccggcaat
     3841 gaaacggact ctaactaatc gaaatatcca gttaatcgca ttaggtggcg caattggaac
     3901 aggattattt ttaggttcag caaaggcaat tgcatcagca gggccagctg ttattctcgt
     3961 ttatttgata tgcggtttgg ctatttatgg tttgatgcgg gccatgggtg atttattttt
     4021 agcaaatagt acatttaact ctatcagtga tttcgttgac tattatttag gcaaaaagtg
     4081 ggcatttttt gtcaattgga cttattggct ttgctggctt ggagctggac ttgcggaatt
     4141 aacagcaata ggattgtata tccatttctg gtttgtacat gtaccagctt tgataagcgg
     4201 aggtagcgca ctaatgatct taatggcagt caaccttgtt gcagtcaagt ggtttggtga
     4261 attggaatct tttttctcaa tcatcaagat cttggggatt ttccttttta tcttattggg
     4321 tataggattg agtgtctcag attttcatac ccagtctatc ggaatcaaag aaaattttgg
     4381 acagatcgat gaattactac cgtttggctt tgctggaatg gtagtctcat ttcctatggt
     4441 tttattcagt tttgctggaa ttgaaatgat cggcttaacc gttggcgaga caagtgatcc
     4501 gcaaaagaat ttgaaaaaag caatcaactg tctgccttgg cgtattctct ttttctatat
     4561 aggaacgatt ttgtcgattt tatttgttgt tccatggcat tgtttagatt taaaagaaag
     4621 tcctttagtg actatgctgc aagtgatcca ttgccaaggc ggagcagcaa ttgtcaatct
     4681 aatgattttg gttgcttctt tgtcctcagc aaatagtgca gtctacagta ctagcaggat
     4741 tttatatcaa acagcaaaag aaaaaagttt gccccattgg ttttgtgcgc tttctaagaa
     4801 aggcgtacct ttacacggta ttttaattac aggaatgtta ttttttgccg gtctttgtct
     4861 acattttttt gtagctgata gtcgattgtt ttttcagctt ttggcaggca tgacaacgag
     4921 ttgttttcta tttgtgtggt cttcgatctt gtgtgcacat gttcaattta ttaaagaaaa
     4981 gaaacggaac actacacaaa gatataaaga tcttgctttg ttggtgtttt ttacttcaat
     5041 tgctttcagc ttgttattcg aaagcacgac acgctttatt ccttttttat tggttatctg
     5101 gtttgtattc ttaaattttg tatataaaag aataagcaat agaaatatca gttaggccca
     5161 gatacatttt gcaaaacgaa ttatttcaga cccccctgaa ataatcgata cgcctgaaaa
     5221 aacttccctg cgtataataa cagtatcaat taaacaggag gggataggat gaactggtac
     5281 agattgacgg tagaagatgt attgaaaaac tatcaaacat caacagaagg cttgtcgcaa
     5341 gaagaacgac agaaacgcct gactgatcaa ggatataatg aaataaaagc aaggcagcag
     5401 acaaaacgat ggaaaaaaat agcaaagcat tttactgatc tgctgatgat cgtgttgatc
     5461 attgcctctt ttttgaaatt tgcttctagt gaatatattg aaggaagtat tattttattc
     5521 gtagtgatcg tcaatggact cgttagttac tggcaagaaa gaaaagcgga agaatcattg
     5581 aatggtttga agcagctgat gggacaagaa gcgatagtac tatcaaacgg gatgcaagaa
     5641 aaaatccctg cgaaagaact ggtaaaagga gatgtcattg tcttacgtgc cggagatgtc
     5701 gttccagcag atatccgatt aatcgaagca catgatttat tagtggaaga atcgattttg
     5761 acgggagaat cagaagcatc tgaaaaaagt catgccgttt tgacagaaga agaatcgatc
     5821 ggcgaccaga aaaatatggg tttttcagga acgcttgtac aatctggttc tgcagtcggt
     5881 gtcgttgtag aaacaggaaa cacgacagaa atcggtaaaa tcaatcaagc attgcaatcg
     5941 gtcgaacaac aaacgacgcc tctgattaaa aaaatcaacc gattaaacaa acaaattttt
     6001 caaggcattc tttgtttgag cctattctta gttattttta cgacatttcg atatggtatg
     6061 gattggcata tccttctttc ggctacgatt gctttggtgg tgtcaatggt acccgaagga
     6121 ttgccagctg ttttgaccat gattctatcc gttggagtac atgaaatggc aaaagaaaag
     6181 gcgatcatca aaggacttcc ttcggtagaa acattaggtt ctatgacagt gatctgttca
     6241 gataagacag gaacattgac aaaaaatgaa atgacagttg tggatgtagc tgcaaaagaa
     6301 gaagcctgcg tgttgtccat tatgaaaaac tgtcaggaat taaaaacaaa agaagaacag
     6361 aaaacagaaa atttaagtgg caatccgaca gaagttgcgc tgattcgaca cacagaaaca
     6421 gccaatctac ctttgaaaaa aatagaagca aagattccgt tcagttctga atacaaatat
     6481 atggcaacga tgcatgcgga ggaaaaagga gcggtgattt acgtcaaagg tgcaccagaa
     6541 gtattatttg ctaaatcaac tctttcttct gcagaacaag aagcatggag tcagacagct
     6601 gcagaatttg cgagaaaagg tcagcgtgtt ttaggatttg cttataaaaa agtagattcg
     6661 aaacaggaac tcacgcatga gactttaacc caattaactt ttgcagggat cgccggattg
     6721 atcgatccgc caaaagaaag tgcagtcaaa gctgtaaagg aatgccaaca agcagggatc
     6781 tcagtgaaaa tgattacagg ggatcacaaa gacacagcaa aagcgattgc ggaacaaatt
     6841 ggcttaaaac atactgccaa agttttagaa gggatcgatt tggatcttat gtctgatgaa
     6901 gagttgattc agcaagtgcc aatagtcgat gtctttgctc gaacgacacc agaacataaa
     6961 ctgcgaattg ttaaagcttt gcagaaaaat ggagaaatcg tcggaatgac cggagacggc
     7021 gtaaatgatg cacctgcttt gaaaagatcc gatgtaggaa ttgctatggg aatcaaaggt
     7081 agtgaagtaa gcaaacaggc agctgatatg gttttaggag atgataattt ccatacgatt
     7141 gcaaaagccg taaaagaggg ccggcggatt ttggataatt tgcaaaaaac catcaatttc
     7201 ttcttgccaa ctgcattagc tcaaggattg atcttgattt gggcacttat gctgaatcgc
     7261 ccgctacctt tgtccccagt acagatttta tgggtcaata tggtaacgac aatcacttta
     7321 tcttatgcac tagggttcga gtcgccagat ccagaaatca tgaagcgccc accaagagat
     7381 ccaaagcaag gaatcctatc tggttatcat ttgtttcgga tcatctatgt ctccctactg
     7441 atcatggttc ctgcctattt attggctatg cagtttgaag ggcaaagctt acagcaaacc
     7501 attcttctgc aaaatatcgt cttagctcaa gctgtttaca tgatcaattg cagagagttg
     7561 tccaagccgt cattgaacca aggtcttttg caaaacaaag cgctttttct ttcattaggt
     7621 atccttgctt tattgcaagg gatggttatt tttctaccag tcggccagca gctgatcggt
     7681 acagtaagtt taacgatgac acaacagtta ttgattggag caaatgtgat tcttctattc
     7741 ttagttgtag aaatagaaaa agggatcatg aacaaactgt ttcgcaaaaa agaaaaaaca
     7801 gctagagaat acagtcgcta gccgttacta taaaaaagtt ttgaaagtga gttcagtcaa
     7861 acgattgagc tcatttttcg ttggaataga agaaatgtat acaataggga tatctagaga
     7921 atctagaggg aaattggaaa tcaagatatc tgttttttca aaagaaatag tggaaagatc
     7981 ggttagttcg ataggtaaga agcagacacg ttttcctaag taatctttta attcctgctg
     8041 caagaacgcc ttccatgaag gttctccttg gaaaataaaa tagacattga acaaattcgg
     8101 tttgatagaa aaaatcgttt gttggatcag tagagacaag agttggagaa agtaatttgg
     8161 ttcattgatt tctatttctt tttttagatc gttcagtaaa ttctctttct gttgatacaa
     8221 gtgtgcatat tgctgatcat atgtttctaa aaaacgatgg tattgacggt ccaatgcttc
     8281 aatcaatatc ggcgattcat gatattttaa tatgaggaga gccaagttat cagtcagaga
     8341 tgtttttttt agttgatcaa gagacagctc tgaagctagt tcctctacaa acgtttcgag
     8401 gacctctaaa aatggagaat gatgcaggca gcgagcggtt tcttgtccat agttattgtt
     8461 gtaattccaa ctttccagaa agcagaaaga agcaaaagaa agctcagtgt ctttaagata
     8521 aataccttcc gttaaataca attttttcaa tgcagagtca tacagggagg agagtgtgct
     8581 gttggtatgg atagaagaat ctaatcggca attcgctttt atacggatcg tattgataaa
     8641 gaaccaacat gttccgaaaa tctcttctgt ttcaaccgct agttcagttt gactgaggtt
     8701 tcttaaaaat tgaaaataat gggattcatg gatcgaataa tcctcgaaaa aatagttgtt
     8761 atgagtgaaa ggaatcagca atcgatgata aaaagtacga atcgcacgct cttttccaag
     8821 aatacaagct gtatatttcg tcaaatcgat ctgcaagtcg taacttgcta aacgctgatt
     8881 gattttagtc acatgtcttt tcaatgtccc ataagaaagc ccatggtcgg ctagaaaatc
     8941 agggacagca actttttttg taaaaagcag atttttcagc agttcgacac tgactgattg
     9001 tttgaaaaaa atctcccaaa ggtcatttgt taataaattt tgctggtttt gcaaactgat
     9061 tcctgcttga tcgctgatga tgtcccagcc ttcgggcagc tgagagcgaa tagattgaag
     9121 gtcagaaaaa atcgttcgtt cggttgtccc tatgatttca gctaattcat ttacggttat
     9181 ctttgtatga ttcagcaatt gttctactaa agaaacatga cgtcgaatat ctttttcggt
     9241 aatgattttt ttcattagtg aatacatcaa tcagactccc ttctattcta agaatacgga
     9301 gaaaataaac ggtggtcaag tttcaactga tttttcaatc attgtttatt tttctggagc
     9361 atttactgat gataagaatt tcatcatgaa cttgaaaaat gataccacta aaatcaaaaa
     9421 ataggtgggc aaacaataat aaacatttta atagtagatg gtagcaagac caacacaggc
     9481 ggattgaaca tttaagagtt gataaaaaag aaaaatatcg aagtatttga ttagcaacta
     9541 cttgtattca tttttctatt cttacgtggt atctttgaat aaagcgaatc aataagaaag
     9601 agatggaagc agatgctaac gaaaaaagaa ctaatcgatc acatcaatga aacatacaat
     9661 ttgtctccag aatatatttt taaaaaattt cctgattatt gcgtattcag acatcaaaaa
     9721 agcaaaaaat ggtttggggt cctgatgtct gttccagaag aaaaaatcta tgatgatgca
     9781 agtgaccata ggatcatcaa tattcttgat ttgaaggtcg atcctgaact tggcggaatt
     9841 ttgcgaggaa aagaggggta ttttccagcc tatcacatga ataaagaaca ttggatctct
     9901 attgatctac gtaaaataga aaagctagat gaactagcag atttgattga tggaagtttt
     9961 aaatcaacgc aatgaatgtc taggacaata gagaagaggt tgtgacagaa atgtttagct
    10021 tcaagaaata agaaggaatt tacgaaaatt gcttctcaaa tttttgggaa atttcggctt
    10081 attttctaag aaattacttc tggaacaccg tttgttcgaa aaaagagctg cgacaagaat
    10141 tttgtcccag ctcttttttt tttattttcc taaataaaat tcccgcatcg tttttgcaat
    10201 cattttgatc aaaggatgat cttctgtgaa gctagaagat aaataaattg ttcgtgtcaa
    10261 gggcaaatct ttgatttccc gcaagacgaa aggatcattt ttatcgtaag accaagaaac
    10321 ttttgggaaa aagctaacag ctaatccttg cttgacaata gaacgtaatg tcgcaggatc
    10381 atctgtcgtc gaactgatcg tcaattcgat acttttcgct tcaagtgctg catccagtgt
    10441 atgacggaag gcgttgctct ttgacagcat caagatgggg gtgcgttgga tatcttctaa
    10501 ttgcagatgt tcttttttag caagaggatg tttcttagga atggcgacat aaacaggttc
    10561 tttcatgata ggaatattgg tcatgccagg cttcatctcc gaagtgatgt acagtacagg
    10621 atcggtaata tcatctggta aatgatgttg catgatcgtt aattggatat caggatgctt
    10681 ttccataaaa attcggacca agttcggaat agaaacagag gctacctcta aatacaatat
    10741 gacggaagta acgatcttct cattgaccga agacaataat ttgattcctt cttctaatgt
    10801 gtctaatgtc ttgactgcat atggataaaa ttcttctgca taaacggtcg tctcaatgtt
    10861 tctgccgcta tggtaaaaca aagggacacc taattcgttt tccaaatgtt tgatcgcacg
    10921 gctcaaggca ggttgagaaa taaataattc tttggctgct tgggtaacat tttttgtttc
    10981 gaatactttt ttaaaatact gcagatgaag caatttcata attatccatc ccatccatac
    11041 ctttttgtta tggttttttc tttttttggc attttttatt atagcagaaa ttgactaaga
    11101 tagcagtgga aaaagtaagt gctgaggagg cgaggctcat gagtacaggt ttcttatttc
    11161 ctggacaagg cagtcagaag ttagggatgt tagcggatat ccctgaaatg tacatggaac
    11221 gtttagaaaa tgtgacgggg tatcggttag ttgataaaga agaaaactat caagatacgg
    11281 tttttatcca gctagcatta ctgacaaaag cagtatttta tttagatgaa atggaaaaaa
    11341 acggtattca gcctgatatt gcagcaggac attcgattgg tgctttttgt gcagctgttg
    11401 cttgtcgatc tttgagattt gagaatgcag cggaactagt ctatcaccgc gcagtcttaa
    11461 tgaaagaagc ttatccaagc ggttatgcaa tgggcgtagt agtaggtgtg acgagaagcg
    11521 aagctgagga aattgtagca aaaacatttg atcctgacca tccagtttat ctgtcaaatg
    11581 aaaattgtcc gatgcaacat acgttatctg ggagtatcga aggtttatcc gttacgttgg
    11641 cagaagccaa acggcaccaa gcgcaaacag cgaagctgct gaaagtccct gtgccatctc
    11701 actgtctctt gatgaatgat acggtcaaga aattcaagcc gtatatgcag tctgtccatc
    11761 tatctgatgc tagatgtttg tatataaaaa atacggatgg aagagccact caggacgcag
    11821 aagagattcg taaagatttg ttggaaaacc tagctttccc tgtgcagtgg aaccaaatga
    11881 tggacttggc aaaagaatta ggaatggatc tgacgattga atttcccccc ggcaatactt
    11941 tgacaaagct gattcataca aaatttggtg tagataaagg gatccgtacg atcaatctag
    12001 accaacatgg tattgatgac gccacgtttc tatatcaaaa atggagatga ctaattatgg
    12061 aaaaaccaaa ccgcaactgg gcttctaaac gcgaaaaaaa agcgcagcga ttggaaaaaa
    12121 tcagcaactt gctcaatggc aagtatgtag aaactgaaaa aatcgtagaa gtaatggaaa
    12181 aactgattgc tcccggcgac aaagttgtat tagaaggcga caatcaaaaa caagcaagtt
    12241 ttctttcaga aagtcttgaa aaagtagatg cagaaaaagt gaatgatctg catttgatca
    12301 tgtctagtat ctcacgacca gaacatttga atatttttga aaaaggaatc gcaagcaaga
    12361 tcgatttctc ttatgctggt gcacaaagct tgcgtgtcgc ccaaatgatt gaagacggca
    12421 caatgaaatt aggtgatatc catacttatt tggaacttta tggacgctta ttcatcgatt
    12481 tgattccaaa tgttgtttta gtagctgcag ataaagcaga taaagacggc aacttgtaca
    12541 caggtttcaa tacggaagaa acaccaacaa tcatcgaagc tgcagctttc aaagatggta
    12601 tcgtgatcgt tcaagttaac gaagttgtcg attctttacc acgtgtagat atccctggtg
    12661 actgggtaga cgttgtcgtg aaagcagata aaccttatca attagaagct ttattcacac
    12721 gagatcctca aaacatcaca gaattgcaaa ttctaatggc aatgatggca atcaaaggaa
    12781 tctacgcaga acacggcgta caatctttga atcacggtat tggattcaat acagcagcta
    12841 tcgaattgtt actgccaact tatggtgaat cactagggtt aaaagggaaa atcgcgaaga
    12901 actgggtatt gaacccacat ccaacgatga ttccagccat cgaatcaggt tgggttgaaa
    12961 gcatccatag ttttggtggc gaagttggta tggaaaaata tattgctgct cgcccagatg
    13021 tcttttttgt cggtaaagac ggcacgatgc gcagtaaccg aacattatca caagctgcag
    13081 gacaatatgc catcgatatg tttatcggtt caaccttgca attagattac gaaggaaact
    13141 cttctaccgt aacaaaagga cgtttatctg gttttggcgg tgcgcctaac atgggacata
    13201 atcctggcgg tcgtcgtcat tctacaccag catggcaatc acttcgcgac aatgatgatc
    13261 cattagggaa aggccaaaaa ttagttgttc aaatggttga aacatatggt tcaaataaaa
    13321 aaccagtctt tgtcgaagaa ttagatgcac tagctgttca aaaacaagca ggcttagcca
    13381 atgcaatgca ccagtgatga tttatagcga agatactaca catatcgtga cagaagaagg
    13441 aattgcttat ttgtacaaag caaaaacaaa agaagaacga caagcagcaa ttgcagcaat
    13501 tggcggtgtt acacctcttg gaatgactag cacgaaagaa tcactagata gcttgcgtgc
    13561 agccggcatc gtgaaattac cagaagatct aggcatcaaa cgtagtgaag cgaaacgttc
    13621 attattagct gcacaaacca tcgatgattt gatcgaatgg tcagatggcc tgtatgaacc
    13681 accaatgaaa ttcagaactt ggtcataaaa ttggcgagaa aaaccttttg tcgccattta
    13741 cccagatgga agaagaaaga cgaataagca gaacgtgtat gctctgtttt acaaaaaggg
    13801 gagagatcca tggaacaatt agcttataaa tatccaacga aacagccgat caataagaaa
    13861 atccatgtag gagtggtcgg ttcaggagat ttggaaatct tgatgtttcc aacagaaaaa
    13921 acagaatcta ctgtaaaaat ttgtacagga agcgatggct tcggcgaagt atggaacaat
    13981 gtattaacga gattttttga tcgttatcca atttcagcag atatcgtgat caatgacttc
    14041 ggtgctacac caggagttgt gtacctacga ttgacgcaag caatggagga actttcagat
    14101 gaaaaatagt tttgtagaat taaacgccag agaacgagtg aacgctctac ttgacaatgg
    14161 acgtgaattg attgatccat tcgatcaaat catcgcgcca agtttaccag cacaaggaat
    14221 cgtccctgaa agtgatgacg ggatcgttgt cagccgtggc acgatcaatg gcaaacaagc
    14281 agtagtaatc gctatggaag gaaaattcca aggtggcggt atcggtgaag tttccggtgc
    14341 aaaaatcgtt gctgcacttg aacatgtgtt agaagaaaat aaaaaaggaa atgagatctt
    14401 tccagtcatt gtactagata caggtggtgt tcgtttgcaa gaagcaaact acggactgtt
    14461 atctatttca gaaatcggaa atgtaatcat tgctctaaaa caatatgtac cagtcatcgg
    14521 tttgattcca ggacgtgtag gatcatttgg cggtatgtcg attacttcag cattaatgag
    14581 ttatttaatc gcaacaaaaa aagcgagagt cggtttgaat ggaccagaag ttattgaaca
    14641 agaagcaggc gttcgagaat ttgattctag tgacaaagac ttgatttgga atacattagg
    14701 atctcgtcaa cgggtagaat caaaaatcat tgatgaatta gtgactgata gtgtagaagc
    14761 aatcaaagct gctgtcatcg cagcaatgga cgaacgaaaa gacacgcatc gcagtgaaag
    14821 ctcagcattt tatttatcat tacttgattc tttagatctt tcaaaaccaa tggccattga
    14881 cgattacaat gaagcaattg ctaaccatca agcaaaatca attgacttac cagaagtagc
    14941 agaaggaaat gaaccagctg cagcaagtgt tggttacgat tggtttgcgg cactaactgg
    15001 catgaaaaat ccaacttctt ctattagtac tgtgtattat ggtaaatcaa gtaaattcat
    15061 agaagaagcc atcgtgacat caatcgtacc tgatcctaaa aacccgatgt accgtgtacg
    15121 aaacggtgaa gttggtttag ttgaaggttt ccgcatggga caaattttgg atcacgttta
    15181 cgaagaagat aaagacaaaa aagtgaaacg gccaatcatc gttgtgatcg atgtaccaag
    15241 ccaagcatac ggctataacg aagaattgat cggtatccat gtggctttgg ctaatagtgc
    15301 tgctgcttat gcaaaactcc gtcaagctgg acatccagtt attggattga ttgcaggaaa
    15361 tgctatttca ggtgcatttt tagcacatgg attacaatca agccgcttga ttgctttaaa
    15421 caataaagaa attaccgtcc aagcgatgtc taaagaaagt gctgcacgtg tgacaaaacg
    15481 ttcggttgct gaaatcgaag cagctgctga aaaagttcca gctatcgctt acgatatcaa
    15541 taattataca aaactaggag cgatctatca tttcctagaa gatatcaccg accaagctgc
    15601 ttcagaacaa aatgtcagca aagtcatcga agcaatcaat gaagccattc aagatgtccg
    15661 caatacaaat gacacgatgc tgacaaatcg atacacaaac gaaacagcca aaacattcgg
    15721 acgtgtcgaa acaaataaag tccgtgccaa aatggacgaa gaatgggatg cataaaaaat
    15781 gattcaagca catgatattg tcctttttga taaacatgaa atagaagggt tcaccttgcc
    15841 tgaatgggtc gttctaactg aaaagatgtt cggaacagtt cgtcgtgcgt cgacggatca
    15901 cgcagaaata ctaccgattg gtttacgtgg acctgcccgc aatcagagat ggtcaacaga
    15961 aatctcaaaa catgccgtca aacaagtcat tcatccgtgg gacatcatcg aacaagaatc
    16021 gtatagacaa gagaaaatca tggattatcc tgtttatcag caatttcatg ctgctagaaa
    16081 catattatca ggatgtaaat ggggaatcgg cggcagctta ggatttgaat tatcgactgg
    16141 gattccggca gtaaaagaaa ccagtgattt tgatttgctg ctgtacgcaa attcaccgat
    16201 tgaattgcca attcaggtaa ttcaatcgca tcctgctttt tttgagcaat tcgataccca
    16261 agtagtgact cggcaaggcg gtttttcact aaaagagtat ttgagagagc cagagaaaaa
    16321 aattctattg aaaacagtcg aaggacctaa attaaccaaa gaactttggt aataaagaaa
    16381 aagagagaga aagagatgtt tgaaaacaaa aaaatcaaaa tcaacgtaag tgacttccag
    16441 aaagtatcag ctgtaacggc tgttatgatg caaacgatat tgagctttgc tttggcaaac
    16501 agccacagcg caacaaatac agcttttctt ggtacctttt acgtcatcgt taaatacaca
    16561 gcaccaatgt ttatttttgc aattgtttat aatatggtaa aaacaagtca gcatttgtct
    16621 tattgggaat ttttgaaaga aaagtttttt gaactagtcg ttccgtatct tctatggacg
    16681 acggcttatc tttggctatt tccagcagtc caacaacaaa ctccgtatac ggatactgca
    16741 agctttttac tgaaatatgt cacaggtgat ggagcgcctc atttatggta taccgttatg
    16801 atgctgcaga tccaattgtt catgccgttt tttgtttggc tcggttacaa agtatttgct
    16861 aataaaaaat atgtatggcc agtattgatt ggttcaacga ttttatatgt cgcatggtat
    16921 ctgttctatc aaacacaagt attgactggg ccttatcatg acagctggta cttattagat
    16981 cggtttgttg ctagtttcat gatttatggg gtagccgcat ttgtttatca cgaaaaagtc
    17041 tatcaatatt tggacagagt acgttatctt ttcttacctg ttgccttagt tatcgcgttc
    17101 ttttctgtcc gttcattgct cgcacatcca ggagacttga gctttgcgaa tgcaccttac
    17161 ttgaatacga tccaaagttt atacagttta gtcatcatct ttgctgtctt cataggcgct
    17221 tccaaaatga ttgtaaacga ttcgccaaaa ttgccattgt tcaaatggtt gtctgtttat
    17281 gcttatcgta cgtatttagc caatgtattt gttttccaag tactgttatt attgtttaaa
    17341 gatagttggc tgcagttacc ttcaggagtc atgattcttg tagcttatct aatgacggca
    17401 agttgtgcat ttgctttaag ctggttactt catatcattt gggtagctat caaaaaaggt
    17461 ttcagcaagt aatagtagga agaaggagtg agtgtcatga taaatgacga acgaaaaaaa
    17521 gcagtgactc agcaactcgc tgcgttggta gaggaagcat tgatcgcaga agtaacatta
    17581 agtccaaaac ctggattagt ggatgcactg gatgcgggtg cacattccga tatggactat
    17641 gctttatttc ttaagtctgc taagacgcta actccttttt ttgaagaaat ggctcaaata
    17701 gcatggcatc atgctattga tcaggagtta agagaaagaa ttgctgagat tgggcgaaaa
    17761 gcggaaaagg caatgctgat tgctacaaat ggcatcaata cccataaagg agcgatttgg
    17821 gcaatggggc tattgatcag tgtcattgca caaaaaatca gtatggaaca gcctttttct
    17881 ttatcgaaaa tacttgatga tgtcagcaag cttgctgctt ttcctgatac aaaatatcgt
    17941 cataaaaaaa ccactcatgg tcatagggtg aagcaaaaat atggcgtaaa tggtgcatat
    18001 gaagaagcga tattagggta tccgcatatc aggcttgcaa ttcaagcagc taaggagcta
    18061 gataatgatt catatgtaca gaaacaaatg catatgttgt tagttttgat gagttcgcta
    18121 gatgacacat gcgtattgca tcgcagtgat cagaaaacgt tagtggaaat gcaaaaactt
    18181 gctcatgctg ctagtcaaag tgctttacca aatcgtccat ttacagcact gttgcacttt
    18241 tgtcaggaaa accaaatctc tcctggagga agcgcagatc tcttgtctgc aagcttgttc
    18301 atgctgaaga tagaggaatt atggactgcc gacacataca tttgtccgat gttactcaat
    18361 gactaaataa gacaaaattg ctaaaaaaac agtttatgca tctggttatt gactcttcct
    18421 tttagcaagg ctatcataaa aatagttttt tgtaataaaa gataacgagg aagacaatga
    18481 aaaaaatcaa ttatttagca atcattttgt tagtgggcct atgtttgttt gctctgccga
    18541 cccaaggatt cgctgtcgcg ggtggtcatg gtggaggtac atctagcggt cgaagttcgg
    18601 gcagaggttc atttggtggt cgttcatatg ggggcagtta ttttggcagt cgttcttacg
    18661 gcagagattc ttatgggtat tatggtccag gtacaggtcc ttctagttat gagcaaacgg
    18721 gatttcttgc attagctgtt ttgtcagtag gaggggtttc tgctttttct atcgtcaaga
    18781 ataaaagaca aaagaaaagg ctgaaagcgg agatattgtc ccgcatgtca ggaacggcta
    18841 aagaaaagaa agcacagttt ctggaaatcc aaaagatatt catgaagatc caatccgctt
    18901 gggaagaaga aactcctgaa aaagccagaa gctgttatac agacaagctg tttgccgaac
    18961 atcagaaagt tctgcaaaaa aacaaagcag acggtgtaag aaatcatact aaaaaaataa
    19021 ctattcagga agtaggaaat tatcgacaga tccacgaaaa tagttttgcc atccgaattg
    19081 atttcagatg ctgtgattat tcagtcgatc gaaaagatgg gcgattgatc tcaggaagta
    19141 agacacaacg acagtatttt tctcagatat ggtattttga tttttctgag aaagaaaaaa
    19201 gatggcaagc cgattttatt cagccgatta tgctggattg acaggaaata tttatgaacg
    19261 gagttgtgac aaaagccttg tcgcagctcc tttttctgaa taaacggtgt gccagagata
    19321 gcctatcggc aataaggcac gattttgtaa aatatgggaa gcgatttttc aaaatcgctt
    19381 cttatcgctc aaggctgaac atctctgtca caacctctcc actaaatggt gtttaaaagc
    19441 ttgcatctgc gataataaga acaaccaaac gaaaaatagg aagcactatt ttagtttagt
    19501 tgttcttatt acttgatgca ggacagcttt ttcacaacct cttttttgta tagatgggca
    19561 aaatttttgt aataataggc gtatgtcttt ttcgttgagg agatgaaaaa gcatatggct
    19621 tattttttgt ttgctttgct tactttcttt ccacttcaag tggcagaaat atctactttt
    19681 tattatgtaa gcggaaccat atacttgaaa tcagaaggag atcatttcta tgaaaacaag
    19741 aacaattgaa atacttcaca gaatcatgac agcccaatat ccagtaaaag caaaacatct
    19801 agcaagtgag ttcaatgtca gtcagcggac gatccgtcag gaagttcttg aagcgaatat
    19861 ctggttgagc agtaaaaagc ttccggagat ccgtacaatt cgcaacaaag gttttttgtt
    19921 gaagctggaa aaggaaaagc aagctcgatt agaaactgaa ataaaagatg tgaaagacgg
    19981 ctttttgaat cggcgcgaac gtatttttga tctagtttta gccgttgctt acagccgaat
    20041 cccgatccat ctaaacaaaa aagaagaaga atttcaaatc tctaaaagta cattagatga
    20101 agatatgaga cggttacgag caacactggt cagttatggg atagagatcg tcagttatgg
    20161 aaagcaagga ctggtttata aaggttcaga acgatctatc cgtacgatga tctatgattt
    20221 tatcaatcag aatctgggaa tgaccgactt ctttatgaat caagatgcac aattaacacc
    20281 ggctcaaaaa atctttctcc attattttcc tttggtggaa atcaaaaaaa tacaggagat
    20341 ctattccaat aagttgttga aacaagaaga tgatatttat aaaaaacaaa tcctgctttt
    20401 tacattgatc tggatccagc gaatcaggaa acatgaattt atctctgcca ttaattggaa
    20461 aaatacagaa ataaaagaca gtagcttttc tgcatttatt gaccacatga tcgaggaatt
    20521 tcaattggac aatgtcccgc aagtagaaag aaattatctt cgtttcacta ttgagacgtt
    20581 caatgctaga gatatctcca attcaatcga gtgggtacaa gctcaattac taacgatcca
    20641 attgatccaa tttgtagaga acgagacaca cgtacctttc catctaaagg aagaagcttt
    20701 attcgaaaat ctctataagc acatggctgc actgattgta agaatcaaga atcatatcca
    20761 agtcatgaat ccattgaaag aaaacatccg aacaaattat ttcccaatct accgcgcagt
    20821 cactcatttt gttcctacga tcgaagaagt aattggtggc aagatactgg aggacgagat
    20881 agcttttctt gtcatccact tttcgacgat cgccagtgta atcaagcgag acttgaatta
    20941 tgtttataaa tcagtcgttg tctgtaacca tgggatggct actggaaatc tgttagcaga
    21001 gaatctgaaa gaaaagtttc ctcagatcga gattactgca gttttaagtt caaaagaaat
    21061 cgaattagta gataaattgg atgtcaatct ggttttcagc acctttcaat tgaattatcg
    21121 aaataagccg cttttagttg tcgatccgat tttaactgag acgaatatct tggttataga
    21181 agactttttg aaaagaaatg aagaactaca gcggttagag cctaacagtc atgaatcgac
    21241 ggagttattc actcaaataa tagatgtcat gaaagaaagt ggtggccgag tagatcgaat
    21301 catctatggc aagttagaaa aattattcga aacaaacaat ttagaaatca acaaaaggga
    21361 gatccagcct atgttaaaag atattttgac ggacagcaat atcatgatca aagaaaaagc
    21421 cactacttgg gaggaatcga ttgaactagc tgctagacca ttggttaaag aaaacattgt
    21481 cgaagagcgc tatgtcgaag cgatgattcg tgcagtgaat gattacggac cgtatatcgt
    21541 gattggcaaa cacatggcac tagcccatgc ccgaccagaa gatggggtca ataaattagg
    21601 agtcagcgta gctacgattg agcaaccaat cgatttcggt aatcctgaaa tggatccagt
    21661 aaagattatt ttttgtttgg cagcagtaga ctcctactcg catctgaaca tcatgaaaga
    21721 actaattgaa ttgatcaaca acgaagagaa gttggatcga ttgattgaat gcacaaatat
    21781 cgaagatttc aaacaactgc ttttttcagt agttgagaaa taataaatga tacgggggag
    21841 aacaaagatg aacaaattga acattttatt cgtatgcggt gcaggtttag gaagtagttt
    21901 tgcagcacag atgtctgctg aggatgtttt gaacaaatta ggagtagaag ccaaactgga
    21961 ccattgtgat atttcttccg cagtttccat gaaaccagat gtgatcatca cagcagaaaa
    22021 ttttcgttcg cagttcgaaa agttcgcagt tgatcctaaa acggcgatga tctatttgaa
    22081 aaatattgtt tcaaaaaatg aaattgaaga aaaaatcacc ccagttctgc aagataaggg
    22141 agttatctaa atacaaaaag aaaagagggt aaaaa
//
LOCUS       DANJMI010000047        21813 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000047 DANJMI010000000
VERSION     DANJMI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.047"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(530..2026)
                     /gene="lysS"
                     /locus_tag="PWU81_002153"
     CDS             complement(530..2026)
                     /gene="lysS"
                     /locus_tag="PWU81_002153"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL1110841.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="PWU81_002154"
     CDS             complement(2137..3141)
                     /gene="dusB"
                     /locus_tag="PWU81_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL1110842.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="PWU81_002155"
     CDS             complement(3142..4029)
                     /gene="hslO"
                     /locus_tag="PWU81_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL1110843.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="PWU81_002156"
     CDS             complement(4246..6357)
                     /gene="ftsH"
                     /locus_tag="PWU81_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL1110844.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="PWU81_002157"
     CDS             complement(6447..6992)
                     /gene="hpt"
                     /locus_tag="PWU81_002157"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL1110845.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="PWU81_002158"
     CDS             complement(6992..8437)
                     /gene="tilS"
                     /locus_tag="PWU81_002158"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL1110846.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8557..9027)
                     /locus_tag="PWU81_002159"
     CDS             complement(8557..9027)
                     /locus_tag="PWU81_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDL1110847.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9073..9498)
                     /locus_tag="PWU81_002160"
     CDS             complement(9073..9498)
                     /locus_tag="PWU81_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDL1110848.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9565..9834)
                     /locus_tag="PWU81_002161"
     CDS             complement(9565..9834)
                     /locus_tag="PWU81_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDL1110849.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9845..11440)
                     /locus_tag="PWU81_002162"
     CDS             complement(9845..11440)
                     /locus_tag="PWU81_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL1110850.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="PWU81_002163"
     CDS             complement(11455..14976)
                     /gene="mfd"
                     /locus_tag="PWU81_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL1110851.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14993..15553)
                     /locus_tag="PWU81_002164"
     CDS             complement(14993..15553)
                     /locus_tag="PWU81_002164"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL1110852.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15906..16880
                     /locus_tag="PWU81_002165"
     CDS             15906..16880
                     /locus_tag="PWU81_002165"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL1110853.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17050..18450
                     /locus_tag="PWU81_002166"
     CDS             17050..18450
                     /locus_tag="PWU81_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDL1110854.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18528..19250)
                     /locus_tag="PWU81_002167"
     CDS             complement(18528..19250)
                     /locus_tag="PWU81_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1110855.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19329..19835)
                     /locus_tag="PWU81_002168"
     CDS             complement(19329..19835)
                     /locus_tag="PWU81_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL1110856.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19958..21757
                     /locus_tag="PWU81_002169"
     CDS             19958..21757
                     /locus_tag="PWU81_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDL1110857.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         6053 a         4752 c         3911 g         7097 t
ORIGIN      
        1 gagtgtttcc cgattcattt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcggc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcgt
      241 tagtaacatg tcaataactt ttttgttatg tttttgaata aatattttct tctgtctttt
      301 ggacaagtaa taatatacca actaaattta gcttggtcaa cgaatttttt gtattttttc
      361 aagttttttt caataaacga ttattcataa cttttctttc tttatcgttc gttttcgtct
      421 tctttaatgt ctagaatagt cggcattagc ttttgaactt tcgttattat ttattccttt
      481 ctacacagca aaagctgcct aaggaattcc ttagacagct tttattcgat tatctcattg
      541 ttgggaatag caaaacatcc cgaattgatt gtgcatcagt tagcaacatt actaatctgt
      601 cgatcccgat gcctagacca ccagttggcg gcataccgta ttctaaggct tccaagaagt
      661 cttcgtctac accgtgagct tcgtcatttc ctagttcacg ttctttttct tgcgcttcga
      721 atctttctct ttgatcaatt ggatcgttca gttctgtaaa ggcattagca aattcgcgcc
      781 ccactatgaa tagttcgaat cgatctgtga atcgaggatc ttctggattt ttcttcgcta
      841 aaggcgaaac ttcgactgga tgtccgtata caaacgtcgg ttgtgtcaat gtttcttcaa
      901 caaatgtttc aaagaattca ttgataacat gtccgacacc catagcttcg gttacttcta
      961 cattgtgttt ttcggctaaa gctaatgctt cttccaatgt catttcttgc cagaagtcta
     1021 caccagtttg ttctttgatt gcatctacca tatggatgcg tttaaagtca ctttctaagt
     1081 cgacagcttg tccatcatat gtgatttttg ctgttcctag tactttttca gctgcgttac
     1141 ggataatacc ttctgtcaga ttcattacat cattgaaatc agtatatgct gtgtatgctt
     1201 ctaacatcgt aaattctgga ttatgtgtag tatcaattcc ttcattacgg aaaacgcggc
     1261 cgatttcata aactttttcc attccgccaa caattagtcg ttttaaatga agttctagcg
     1321 caatacgcaa atataaatca atgtccagtg cattatgatg cgtgataaat gggcgtgcgg
     1381 cagctccacc tgcctcattg tgcaatactg gtgtttctac ttcaatatag ccattgccat
     1441 ctaaatagcg acggatttcg ctgataattt ggcttcgttt cataaatcgg tcaaagcttt
     1501 cgcgattact gattaaatcc aagtagcgtt gtctgtagcg ttgttcaata ttcgttaagc
     1561 catggtattt atctggcagt ggacgaagtg cttttgacaa aatcgtgatt tctgaagctt
     1621 tgatagaaac ttcgcctgtg tctgttttca tgatttgtcc agttacacca aagaaatctc
     1681 caaggtctgc atgtttgaat acttcatagg cctcatcacc tacttggtct tttcttacat
     1741 aaatttgaat ttggccttca cgatcttgta gatgcgcaaa tccagctttc ccttttcctc
     1801 ggcgagtcat catacgacca gctacactag cggtcaatcc catttctgct agttcttctt
     1861 ttgttcttgg atcaaatagt tcatgcaatt cttctgagtt gtgcgtgcgt tcaaatcgtt
     1921 ttccaaatgg atcgattcca tcttcacgca attgttccat tttttgacga cgtacaagca
     1981 tttgatcatt taaatcttct acttgatgtt gttgttcctg tgtcacaaaa gttcctccat
     2041 tctcatgtta atctctttcc tataatgcca atgaaacaca aaaaaagcaa gccgaacttg
     2101 gaatttcggc ttgaaaattc acgaaaaact tagaaatcac aacgtttgtt tcaatagtac
     2161 tttttccgct ttttctacaa attgatccag caggtcattg atttcttgct gtttttctgt
     2221 ctgattgatt gctactttca ctttcgctgc tcgaggaatc ccttttaaat aataagccgc
     2281 atgtgttctg aattctctac aagctacttt ttctcctttg agatctgcta agcgttgtaa
     2341 atgaagttta gctgtagcaa ttttttcggc tggtgttggt tcaggaatca gttcccctgt
     2401 ttcgagataa tgctgtgtgc ggtgaatcat ccatggattg cccagcgctg cacgaccaat
     2461 catcacaccg tctgcaccga catagtcaag catccatttt gcatcttctg gcgttttgac
     2521 atccccgttc cccataaatg gaatcgtcaa atgttttttc acttctttca atacttccca
     2581 gtccgctttt ccttcataca tctgaacccg tgtacgtcca tgcatagcga tggcacttgc
     2641 tccagctgat tgtgctgcta atgcattttc tacagcaaag acgtgctctt catcccaacc
     2701 aatacgcatc ttgattgtta ctgggatatt tactgctgaa gaaacagctt ggaccatttc
     2761 gtatactttc tcaggatcta aaagccattt tgctcctgct tctgctttga ttactttatt
     2821 tactggacag cccatattga tatctattat atcggcagct gtgttttctt cgacaaattg
     2881 agcagcttca actagactat ctttatttcc gccaaatatc tgtaaactta atggatgttc
     2941 tgtttcttca atgtggagca tctctaaagt ttttttgttt ctgaaatgaa taccttggtc
     3001 actgatcatt tcacatacga ctaagcccgc accaaattct ttgacagtga ctcgaaatgc
     3061 tgaatttgtt attccagcca tcggtgcaac aacgacacga ttagggattt ccacattgcc
     3121 aattttccat gtgtgttcca cttaaatccc tcctaaggct tctttcttca atccatctaa
     3181 ttcttcttcg ctatacttgt atttgttatt acagaaagaa caaaccgctt ctgcaccgtg
     3241 atcttcgtct atcattgctt ggatttcttc tgcacctaac gcgataattg cttctgcaaa
     3301 tttttctttt gaacagtcac atttgaattg aacaggcatt ttttccaaaa tgttcaattc
     3361 ttctgtacca agcaatcctt tcaatatttc ttctggtgtc tgtccttcat ctaagagggt
     3421 agagattctt ggtgtcgttt ttaattgttc ttcgatcttg ctgattgttt cttcatcggc
     3481 acctggcatg atttggatca tgaatcctcc cgccgtcttg atgctgtcgt cagtattgac
     3541 caatacactc agtccaacag ctgaagggat ttgttctgag atagcaagat agtaagtaaa
     3601 atcttcagcg atttctccag aaacaatcgg cgtttgccca gaaaaaggtt cttttaatcc
     3661 taagtctttt gtgacagtaa acattccttg tgttccaact gctccccgca catcgatttt
     3721 accttcacta tttagaggaa gactgagatg cggatttttg atgtaacctt tcacatctcc
     3781 ctttccattt gcatctacta caattcctgt tgctggacca tcgccttgga ttcgaacagt
     3841 tagtttgtct tctcctttga gcgtcgcacc aagtagcaga ctaccgacca atgtccgtcc
     3901 caaagcagct gaggaggtgt gccaagtatc atgacgtctt tgtgcctccg ctacggtatt
     3961 tgttgcttga acggcgtatg ctcggacgaa tccgtcgtga gcaagtgcct taaccaaata
     4021 atctttcatt tgttatgtac tcctttttcc gtttttaaac tcatagtgaa tcatactagc
     4081 aactactgct tttttcaact ttggaatgct tcttttaaaa ataattcaga atcatctaga
     4141 aaacaggaca tcaccctatt tacaacttaa ataaagaaat ctcccgaaaa ccggtttttg
     4201 ttccggtttt cgggagtcat atcagatcaa ttgacctata tttttttatt tgtattgatc
     4261 gtctgaatga ttatcttctg attgttgaga atctgaatca tgttctgctt tattttcctc
     4321 attcagttca tgttttgctt cttcaaaatc acgtttttct tccgcttgtt tttcagcgtc
     4381 tttttcttcc aaagcacgtt ttgcttcttc aaaagttttc gcttcttttt cgcttggata
     4441 atcatttgac tcagtacctt caggcatcac accgtgttca aacaatgact tgattgcttt
     4501 ggcatccaat gtttcgaatt ccaaaagttt ttcagcaatc agtttatgct gtgcacgatg
     4561 agcttcgatg atttctcgag ctttatcatg agcttccatc aagatttttc gtacttcttg
     4621 atcaatctcg aaagcgactt gttccgagta agcttttgtt tgaccatagt cgcggccaac
     4681 aaacacttga tggtttcctt catattgaac aggacctaaa cgatcactca ttccatattc
     4741 tgttaccatg cttcgtgcta gagctgtcgc ttgctcaaag tcattggaag caccagttga
     4801 ttggacattg aagatgattt cttcagctgt acgtccacca agtaagccta cgatttgttc
     4861 aaacatatct tctttcgtca tcaagaactg atcttctttt ggtaaagcga tcatatatcc
     4921 gccggcgcgt ccgcgaggga tgatcgttac tttgtggacg acccgagcgc gactcaatac
     4981 taatccgaca atcgtatgac cagcttcgtg ataagcaacc atttcgcgtt cttttttgct
     5041 gattacgcga tcttttttag ctggacctgc aatcacgcga tcttccgctt catcgatatc
     5101 agaagcatcg attttctttt tatttcttct agcagctact aacgctgctt cattcaagac
     5161 attttctaaa tctgctccgg caaaacctgg tgtctgttga gccacaactt tcaagtcaac
     5221 gtcatctgcc atcggtttat tccgtgcatg gactcgcaag attgcttcac gaccttttac
     5281 atctggacgt ccaactaaaa tctggcggtc aaaacggcct ggacgtagta acgctgggtc
     5341 tagtacatca gaacggttgg ttgcagcaat gacgattact ccttcatttc cgtcgaagcc
     5401 atccatttct acaagcaatt ggttcaatgt ttgttcgcgt tcatcatgtc cgccgcccat
     5461 accggcacca cgttgacgac ctaccgcatc aatttcatca atgaaaatga tagctggtgc
     5521 gtttttcttc gctgtttcaa acaaatcacg aacacgactt gctccaacac cgacaaacat
     5581 ttctacgaaa tcagaacccg agatcgagta aaacggtact ccagcttctc cggcaacagc
     5641 ttttgctaat aaggttttac ctgttcctgg aggtccttct aaaagtacac ctgccgggat
     5701 acgggcacct aattcaacaa atcggcgagg atctttcaga aattcaacaa cttcaactag
     5761 ttcttgtttt tcttcttccg cacccgctac atcagaaaaa cgtacgcgat tagctttttt
     5821 gtctgcttct ttggcttttg atttaccaaa gttcatgaca cggccgccac cgccaccgcc
     5881 gccgccttgt tgtcccatca tcatgtaaaa gaaaaagatg atgattacga ttggcaagaa
     5941 gctaagcaat agggaaaccc atataccgct gcttgattct tcttttactg ttgtagcaac
     6001 tttgttttct tttgccagtt ctgttacttg gctcaatgtt gtatcatttg gcaaaatgat
     6061 tgttgtgaaa tgggtagtag tcgtatctgt tgaacctaag atagctaacc caccgctgtt
     6121 agcgacggtc tgctgttctt tgtattcacc agtgatctta tatacaccat ttgcaggctg
     6181 cacttcaaat tctttgatct tgcctgcttg tagttgttct gtgaattttg agtactcgat
     6241 atctgacgat cgctggccgt tattaccaaa aatgaaataa acgaccatta ccatcgctaa
     6301 gattacgagg acataataca aggcattttt catcatgccg ttgttctttt tgttcatagt
     6361 tgccctccct aagcgtcaat ttaatttaac agttttttat caatcctgac cattctattg
     6421 taccataatt taattgagat acagcattaa tttgattgat aaacactagg tttcaataca
     6481 ccgatgtacg gaaggttacg atatgcttct gcataatcca agccataacc gaccacaaat
     6541 tcatttggta cgttgaagcc aacataatct gcttcgatat ttactactct tccttcaggt
     6601 ttgtcaagta gtgtaacaat tttgactgat tttgctttgc ggtatttgaa aagatccact
     6661 aaataagcaa gtgttcttcc gctgtcaatg atatcttcaa cgatcaacag atcgcgacct
     6721 tctacattcg tatctaagtc tttgacgatt tttacttccc ctgacgagac tgttgcattc
     6781 ccgtagcttg aaacatccat gaaatccaat tccaaatacg tatcgataga acgaacgata
     6841 tctgccatga atggtaccgc tccttttaag acacctacta ctaggggatt ggtgttttgg
     6901 taaatttctg ttaactcttt gcctagttct ttgcaacgaa cttgtatctc ttcttgtgag
     6961 atcaaaattc tctcaatatc cttttccaac atcaggttct ccttccaatt gcttcttttt
     7021 gatatttgta aagaagtatg tagtgtattt tatcagtttc ttcacgaata ctcaaatatg
     7081 aaaaggcaaa aggtatcaag ctatatatat ttcttttggc atctgtaatg atccaagcat
     7141 gctgcctttg tgaattaggt actttctgat cgataaaata tctagatact ttttttgtga
     7201 gattctctgt taatttaatg cggtctcctg cttttctttt ttcgacatac agttgcacat
     7261 cagcaggaat ccaaatgtca tgagaaaata gagaccatga atctgttgct ttgatgtccc
     7321 ctgcattttc aggtttgacc agaccaatcc attctgtttc attcaaatat attccttgtt
     7381 ctggaatcag caacaagcgc tggtcttctg tctggctagt tgcttgtttt tcttttttag
     7441 agagtacaac aaaatgatac tctcttttca gtatccaatt attgccaata ctgagttgcc
     7501 attggctttt tttctgttgc cactggtcta aaatcgactc catttgttgt tctttcaaaa
     7561 taattcctgt ttttgagaat gctttgtcga aaaaggagta aaaaaaatag tatcgttcag
     7621 catctgtcat ttcttcaatc atttctgcag aaaatcgaaa ttgttcctcc cactgttcga
     7681 cttccttttc gatcaactgt cccatatatt tttgaatgac ttggtctgcc cattggatct
     7741 gttgcgaaaa ttgctggaaa tgtctcaacg cctgtgtatt ttcttgtttc aattgaggaa
     7801 ctaccaaatg acgaagccga tttctctgga tatctaacaa ttggtttgtc tgatcctcaa
     7861 agtaaacaag ctgcgattcc gctgcataag tatataggtc ttctttgcta aaactcagca
     7921 acggacgaat cagtctgccc gtagcaaatg gttgtacttc tttgattcca gaaaaagtat
     7981 ttaactgtcc cccacgaacg attttcatta acaccgtctc gatttgatca tctccgtgat
     8041 gagcagtcat caacgtgtca tattgtcctt cttccatcac gcgagtgaat tcttgataac
     8101 gaaagttacg agcttctgtt tctatggcgg atttggctgg atcttcccaa atacttacat
     8161 ataaagggag attcttctct tcacaatatg agcgaagata ggcagcttct tctgctgatt
     8221 cttctcttaa ttggtgattg atgtggacga tacctaaaga aaaaccgatt tggcgttgca
     8281 cttgtaaaag taaatggagc aaaaccatcg aatcaacacc acctgatacc gctgctaaaa
     8341 ttctcgatcc ttgttgccag aatccttgtt cattcccgtg ccgaataaat gactgctgta
     8401 atagcggtgc ttttttcccg cctttttcaa cgttcatcct ttccacaccc tttcttccta
     8461 aaaaaaagag agcgggaaaa cgtctaaaac gacaattaac ctgccctctg ttgatactat
     8521 gacccaaata gtcgcactat cattatttgc ggctttttag ctgcgacgtc cgccacggcc
     8581 accgcgcttt ccttctgtgt ttcgcttcaa tgaagataag cggtcatcgc tatctttcaa
     8641 aaatgaactc atcaatgaat cgaagtcttc tttttgagct gaaggttgac tgcgagaaaa
     8701 agattttttc tgtgctggac gatttcggtt gtcacgggga tctttttgtt gatatttttt
     8761 aggacggctt tcttctccag attcagcagg ttgttcctgc gctttccgga tagatagtcc
     8821 gatttttcca tcatcattta ctgaaataac tttaactgtt acttcatctc ctacacttaa
     8881 aacatcatgg atgtctttta catagccatt tgacacttcg ctaatatgaa ctagacccgt
     8941 ttttccagca cctagatcaa taaacgcacc aaaatttgtg attcctgata cttttccttg
     9001 cagttttgct cctacctcga ttgacataaa aaaaatgttc ctcctaatta tttccctttt
     9061 ttatattcct gcttattttg cagttgtaga actagttgat tgatctgttt catttgtcga
     9121 gctgtctgta tttgattcta atataggata aactttttca ccgtcttttg agtaaaagaa
     9181 tttgcttctg gcaagtttag ctacatactc atcatctttc agcaatgaaa catcttggtg
     9241 cagctgatca acgtctgcgt tgacttcttt catttcagta tttgcttcga ctctcaattc
     9301 gttcaattga tgcatccttt gcatgtcatg gaaaatctgc acacctatta tcgcgaaaat
     9361 gaccaaagca ccaacaaaaa ttgcagccag tctgcgtctt cgaaaaatca gttgacgttg
     9421 ttgttgctga aattcagcat actttttctt tgcatattcc gtatctagcg cagcgatttt
     9481 attgcttttt ttcgtcactt ggattcgctc caatcttttt gattgtcttt ttcattatac
     9541 caatatcgtg aaagcttgtc taatctattt ctctagtgtt tctgtgcgat tttcagaaat
     9601 aatttcgtac attttttgtg catcttcttt tttggttgat tcatgtaatt ccgtaatttt
     9661 cacttccaac gttttgttgc caaactggac tttcaaaata tcgcctactt tgacatcact
     9721 ggaagatttc gccaatttac cgttgatttg aattcttcct ttatcagcta cttcttttgc
     9781 tactgatcgt cttttgatga ttcgggaaat ttttaaaaat ttatctaatc gcatatctta
     9841 ttcctcactt tttaattttt ttatttaatc gcaggatttt cttaccaaaa ggcaacatta
     9901 gccattctcg tactgtaaac agacgaagac gaatcactgc ataaatgaac acagaagccc
     9961 cgatcccaac tcctatcacg ctgacaaaaa aagcagtgga acgatgagtt acaggaccaa
    10021 atactatatt caatacacca taatacagca gcagtactac tgtcatacaa agaaggctga
    10081 acatcaactt ccgtccaaaa tgccgttctt tccaaaaagc atttagctct tttgtttcaa
    10141 ttcgaacaaa ataccaaagc gtcacaccta atcctaaaag ggtagacagg cttgcacctg
    10201 ctgttcctaa taaaccggtc agaaaagaag tagtcaatcc ttttaccaat agtccccatc
    10261 ctgcgccttt taaagaaggg cgaaacgagt tcttgctttg agcaatactt tgataagctt
    10321 ggatgactgc agtgaaagca atggaaaaaa caaataatac aagcgctgca tttcccgcat
    10381 aatctttaaa caacgcataa ttgatataag ggagcagcaa ggctaatccc aaagacgcag
    10441 ctaatgctaa agcggtcgtc aatctaagat agattttggc tgtctgtaaa aactgacgat
    10501 agatccggtt ggtcaaatga cgagtcaacg caggcaggaa tgtcgcactt aatgcagtgg
    10561 caatcactaa acctaattgt actaaaggct gacctcgatc atagacccct tttgctatct
    10621 tcgcaccgta ttctgacagt ccgtaaactt ccaaagcatt ttttacaaag aaagagtcga
    10681 tcagctggaa aaagataagc aatccactat aaatcgaaac cagtccgcct tctaccagaa
    10741 aacgccggat caagggcttt gacggctgtt tgaacaatgg aaaccgtcgg aagcttaacg
    10801 ctcctccatg aatcttttgt tcatagtagt gaaggatccc atatgcgcat aaaccgccaa
    10861 aaacagctcc actcattgct gccgtccctg tttggtacac cgtccatcca agctttctaa
    10921 aagcaaacgc actacagaca atcacaaaaa cacgaacgaa ctgctcaact acttgagaaa
    10981 cagcagttgg ttccatcaag aatcttcctt ggaaattccc tcgataaaaa gatagaccag
    11041 gcatcagcaa aaaggtaaag gaaactactt ggatcagcgg tttcaattgt atgtctccca
    11101 tcatccaagc aatccagctg ctaaatgcaa agacaaacaa ccacaaaaag acacctgtcc
    11161 aaaatactaa gggataaaga ttttgcaaag cttgctttcg ctcgaaagga ttctttcttt
    11221 ctgcaacgta ctttgatatg aactgcggaa gaccggataa agctaaagtc attgctatcc
    11281 cataaattgg ataaacttgc tgataaacat aaaaaccttc gtcgccgact aagttttgga
    11341 ggggaattcg gtaaagggca cttaatactt ttgctatgaa agaagcaatc gtcaatacaa
    11401 atgctccttg catgacgcgc cgcatttctt tatgatccaa atcattgctc cttcctacgc
    11461 tgacagatat ttttgctcac gcaacgcttt gacaaacttt tggatttcct gaagccatac
    11521 tgcttcttcc atttggttgg ggatcgtcaa gcgtatcacc atctgttctt tttcaacgcc
    11581 tagactggct tttagttttg tcgctgtcag tgcttcgaat aactgttcca cactatagcg
    11641 ttttgttcca actttactta gagtaaagac tatatgcgcc tcctgtttcc ttatcgtttc
    11701 taggagcgct cgatcgccat ccattttgat ctgcccaatt gtcaagagat gtgctacttc
    11761 ttctggatat tctccaaaac ggtcgagcag atcatcttct agttcatcta gcatttcttg
    11821 cgaatcaagt tcacgaattc gtttatagat ctcgattttc tgccgttgat ctgccacgta
    11881 tgtttcaggt agatacgcat cgacaccgag gtcgatctca acagtggtct tctccacttg
    11941 actattcttt ccttgtttac gagaaacagc ttcgcttagc atttgagaat acatatcgaa
    12001 accgactgca tcaataaatc catgctgctg agcacctaag agattacctg caccgcgaat
    12061 ggaaagatcc cgcatcgcaa tcttgaatcc agatcccagt tctgtaaaat ctttgattgc
    12121 ctgaagacgt ttttcactga cttcgttaag gatcttctgc tgctcataca taaagtaagc
    12181 atatgcgaca cggctgcttc ttcctacccg ccctcgcaat tggtataacg tggataatcc
    12241 catataatca gcgttctcaa cgaacaacgt atttgcgttc gggatatcta ctcctgtttc
    12301 aatgatggtt gtagtgacaa gaacatcata ttgaccttca ataaattcaa aaagcgtatt
    12361 ttctaattgc gcctctgtca tttgcccatg agcataaccg atccgtgctt ccggaacgag
    12421 ttcttggatt tcttctactt tttgttcgat cgtttctacc cgattatata aatagaatac
    12481 ttgtccacct cgtcccattt ctcgatggat tgcttcgcga atagccccag gatttttttc
    12541 cataacatac gtttggatcg gataacgatt cgcaggcggt gtttcgatta ctgaaagatc
    12601 ccgcacgcct aacatcgaca tatgcaaagt tctcggaatc ggtgttgcag tcagagtcaa
    12661 aacatccact tgtgcacgta actgtttcaa tcgttcctta tgttttacac caaaacgttg
    12721 ttcttcgtca atgatcagta gacccaaatc gctaaattca atatcttttg acaaaattcg
    12781 gtgggttcct acaacaatat caacttgacc tgtacgcagt tgttcgatgg tttctttttg
    12841 ttgctttctt gttctgaaac gacttaatag cccgatattt acggggaaac cttcaaatct
    12901 ttccagcatc gtttcatagt gctgctgtgc caagatcgtt gttggaacta aaaaggctac
    12961 ttgtttgctt tcctttatcg ctttaaaggc cgctcgaagc gctacttctg ttttcccata
    13021 accaacatca cctacgagta gtcgatccat tggtttttct ttttccatat cccgtttgat
    13081 ctccgctgca cttctcaact ggtcatcagt ttcagagtag gggaaggcat tttcgaattc
    13141 tttttggtag gcgtcatcag gtccaaaagc atatcctttt tctgactctc ttgcggcata
    13201 taatttgatt agatcgtcag cgatatcctc gatttttgaa gagacttttc gcttggtttt
    13261 cgtccattca cttccgccta gcttattgat gcgaggcgct ttggcttcag aggcgacata
    13321 tttttggatc agattcagtt gagtaacagg aataaacagt ttgtcgtcgt tttgatacaa
    13381 aatagtcata tagtcttggt gaacgccatc tacttctagc gtttccatcc caatgtattt
    13441 cccgatacca tgattggcat ggacaacgta atctcctgct tttaactcat tatagctttt
    13501 caatcgctct gcgtttgtta ctgtttgacg tctcgtttgt tttttcgtcg ttttttggaa
    13561 aatttctttt tctgtgatag ctactatttt ttcttgagga agttcaaaac cagcctgcaa
    13621 agctccttca actaattgta cctgtcctct gatcaatgca tttttttctg tttctactac
    13681 taatatgtct tcatctcgaa gcatttgttc tgctttgcgt attctgtcct ttgtcggaat
    13741 aaagatcaca acagtctgtt tttgcttgcg ccaacggtcc atttctgttt tcagcaatgg
    13801 catttgtccg aagaactgtt gcatcgtccg atattgaaaa ttatggattg cttggaaacg
    13861 aagatttccc atcccttttt ggaagagtga gaaaaaggtt gtggtgaact tttccttctg
    13921 cacgattttt tgtatatctg ctgaaaatgt ctgctcagaa aacacccgca attcgctgat
    13981 tttttgagtc tgccattctg ccgcttccct ttcgatttcc cgatttgttt ccatgattcg
    14041 ctgataatca tcaacgacta gtaaactatt tgaagggaaa taatccaaga tcgtcacaga
    14101 ttcttggtaa agaagatctg agtagaattt tgcttcttct gtcgggacac ctgcttgcca
    14161 gcttgaagtc agctgaccga agtaatcttg caaaaactcg cggtctgctt cttctgtggt
    14221 cgcagcaagc cgtttttcta gcattttttg catttgctct attccatgtt ctagatcctc
    14281 tggtccgtag acttgttcac tcgttgggga aacccagatt tcttccgttt tttcgattga
    14341 gcgttgtgtt tctgcttcaa aataccgcat agaatctatc tctacatcaa atagttctac
    14401 acgaacagga taggttgtcg ttaacgggta aatatcgata atgcttcctc gaatactgaa
    14461 ttctcccggt tttgcgacca tctcttctcg tacatagccc atcaaagtca attggcgagg
    14521 aagattttcc acgtctatct catcaccaat atgccaattc aattgcgcct gtagccaagt
    14581 tttcttattc ggcagtcgtt tgtgcaaaga tgcgactggc aacaaataga tacctgcttt
    14641 tttttcttga agtgcgttaa gcgttgcaac tctctcgcct cgcgcttctg gagaagaaaa
    14701 ggccatttca gcagaaagca cttcatccac tggaaataaa tggatctgat cttgcggaat
    14761 cacatgttgc agatcttctg ctaactgatt cgcataatac aaatttggca tcagaataat
    14821 catgttttta tttgttttct taaacgctcg agcaaataga agtgttttcg ctgagcccgc
    14881 taatcctgtg attagttgtc taggcatttt ttcttctaaa ttgctgaccc attcttttac
    14941 ttgcggatct tttccgacta attgaataat atccattcct tttcctcctt cctcaattga
    15001 atcgattcat cgcatccacg aacgaatgcc cttctatgag atattcgact gctttaacag
    15061 attgacacat gctttcttct atcaaaggct gattttcttt gctgaaagga ctaagcacat
    15121 gctggactac tgtttttttc ccttctggcc tgccgattcc caccttgatt cgatcaaaga
    15181 cattcgtatt caaatgggaa ataatacttt tgataccgtt atgcccgccg gcacttccct
    15241 tttgacggag ccggattttt cccactgcca gatccaaatc atcgtagatt acgactaatt
    15301 cctctggata aatcccaaaa taagtcatta gtgggccgac tgcacgacca gattcattca
    15361 tgaatgtttg tggtttgact aaaaggatct tttctccatt atgaaaaaat tcggcaactt
    15421 ctgcttcgaa cggatttttt ttgaaagtag cttgatgctc gcgtgcaatt ttatccacga
    15481 ccataaagcc gatattgtgt tttgtatatt ggtatttggt acctgggttt cctaacccga
    15541 cgatcatttt catttttgtt ttccacctaa ttctaaaaag cagcacaaaa ggctggactg
    15601 ctaattagcg gtccagtcca tcgagctttt ttctttttta cacttgtcaa tgcatagtat
    15661 aacacaaaac aaggcgtttc ggctaaaaga gaatcgttaa taaatgataa agaaattaaa
    15721 aatttcttca aagtttaatg aaataattga tatataaaaa tagtgaaata acgcacatgt
    15781 aagcttttac atataataca accttgttat ctgttatatt ttatagccaa atagcgtgac
    15841 atggggcttg agaaatgtta gcataagcat gggaaaaaac aaatcaatgg aaggattgat
    15901 actaaatgac tgcagctgca gaaaaaaaag atcatcaaaa agttattctt gttggagacg
    15961 gcgccgtagg ttctagctat gcctttgctc tagttactca aaatatcgct caagaagtag
    16021 gtattgtgga tatcaatact gctaaaactg aaggagacgc aattgacttg tctcacgctt
    16081 tggcatttac ttcacctaaa aaaatctatt ctgctactta cgcagacgca catgatgctg
    16141 acttagttgt tattacagct ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag
    16201 taaacaaaaa cctaaaaatc aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta
    16261 acggtatctt tttagttgcc gctaacccag tagatatttt gacttactca acttggaaat
    16321 tctcaggttt ccctaaagaa cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt
    16381 tccgtcaagc acttgcggaa ttagttgacg ttgatgcacg taacgtccat gcttacatct
    16441 taggtgaaca cggagattca gaattcccag tttggtcaca tgcaaatgtt gccggcttgc
    16501 aaatctatga atgggtaaaa aacaacccag aaatcgatga agaagctatg gttaacctat
    16561 tctttggcgt acgtgacgct gcttacacaa ttatcgagaa aaaaggcgct actttctatg
    16621 gtatcgcagt agcattagca cgtatcactc gtgccatctt agatgatgaa aatgctgttc
    16681 ttcctttatc agtattcatg aatggcgaat atggattgaa cgacatctac attggtgcac
    16741 ctgctgttat caaccgtcaa ggtatccaaa aagtaatcga aattccttta agtgacagcg
    16801 agcaagaccg tatggctgct tctgctaagc aattaaaaga aattctagat gaagcttttg
    16861 ctaaactaga cgctgaataa tctttgaaca tgacggaaaa catctattcg gtgttttccg
    16921 tttttttatt ttatcaccct tatggtttct aaattttttc ataaagtaga gataaccttc
    16981 gtcttttcct ctaaattatg ctatcatgag accgttccac cctaaacttt tacttaagga
    17041 gttgtcgata tgacaagatc ttcgcatcga aaaaaatcga aacatgttgc tttgctttca
    17101 gttcttggtg ttctcttagt gctgatcagc ttttatacct atcgcagtac atactacaca
    17161 aagcattttt tgccgaatac agaaataaat ggaataaatg tcagcaatct aacagtcgaa
    17221 aaagcaaatg aaaaattaaa agaagcttat tctgataaaa aactggctat taaagaagat
    17281 ggaaaagtct ggcaggaagt accaaaatcc gagctcggct acaaagatga tttcacttcg
    17341 gagctttctc cgatcctaaa tgaacaaaac ggatggactt ggggaatgac ttatgtctca
    17401 gcggcagaaa aacaagaaat agatcctgtc agtcaaaacc accaaaaact agacactacc
    17461 gttcagacat tgacaactaa gctgactgat ttgaacaaag accggacacc tactactgat
    17521 gcaacgatcg aaaaagcagg tgactctttc gttatcaagc ctgaagtcaa tggaaatacg
    17581 atcgacgtcg atgctgcggt caaacaatta aaaagtgcgg taaactctgg taaagataca
    17641 attgaattga ctgaattcaa agaaaaacca aaagtaacat ctgaagacag ctcactgaaa
    17701 gaacaactcg catccatgaa tgctatcgca aatgtaaaag ctacttacag tataaacggt
    17761 gagactttcc agatcccatc aagtgatatt atgagttggc tgacctacaa tgatggaaaa
    17821 gtcgatttag atacagaaca agtacgtcaa tatgtaacag atttaggcac aaagtataac
    17881 acaagtacga atgacacaaa atttaaaagt acaaaaagag gagaagttac ggtcccagtt
    17941 ggcacttaca gttggacgat ccaaacagac agtgagacag aagcattgaa aaaagcgatt
    18001 ctggcaggcc aagattttac tcgttcaccc attgtgcaag gtggcacaac cgctgatcat
    18061 ccattgatcg aagatacgta tatcgaagtt gatctggaga accagcatat gtggtattac
    18121 aaagacggaa aagtggccct tgaaacagat atcgtttccg gaaagccaac tacacctaca
    18181 cctgctggag tcttctatgt ttggaataaa gaagaagatg caactttaaa aggaacaaat
    18241 gacgatggaa caccttacga gagtccggta aactactgga tgccgattga ctggacaggt
    18301 gttggtatcc acgattcaga ctggcaacct gaatacggtg gagacttatg gaaaacccgc
    18361 ggttcacacg gctgcatcaa caccccacca agtgtaatga aggaattatt cgggatggtc
    18421 gaaaaaggaa cgcctgtatt ggtcttctaa caaaaataat atataaaaag aaagcacccg
    18481 tacgattggt acaggtgctt tctttttatt atctaacaat gtgctactta catttcagca
    18541 gcagtaaaga cttctgaaac atcatcatcg tcttctagct tatctactag acgttccaat
    18601 tgctcttttt gttcctcatt taaatcaatc gtcgtttgtg gaatcatcgt caattctgct
    18661 tgcgccaaag tgaactcttt ttccaactca tctcgtacag cagcaaaatc ttctggtgca
    18721 gtataaatct cgaatacttc aggagaagtt tgcagatctt ctgcccccgc ctctaataca
    18781 tcctcaaaca tcgtctcttc atcaacagtc aacccatcgc gttcaatagc gatatatcct
    18841 ttgcggtcaa acaagtaact gactgaaccc gtttctccca gtgatccacc gtttcgagta
    18901 aaagctactc ggacatttgt cgctgtccga ttacggttat ctgtcaatgc atggactaag
    18961 attcctactc cacccggacc atatccttca tacgtgattt catcgtaatt cgtttcatct
    19021 acggcactcg tagccttttt aatcgcccgt tccacattgt cgttaggcat atttgcagct
    19081 tttgctttgt ccacaacaag ccgcaaagct ggattcatag atggatcagg accgcctgct
    19141 ttagctgcca tataaatttc acgagaaagt ttctgaaaga ctttccctcg ttttgcatct
    19201 tgggcatttt ttcttccttg gatattatgc catttactat gtcctgacat gatttttcct
    19261 cccttgttct tttgagaata cacttctatt ctagtactat ttcacaaaaa aacaaagcca
    19321 cagccaatct ataaacgctt cttgaatgcc gcaaaaagca cacataatat cacagcagtc
    19381 agagcaccga tcgaatccaa catgacatct tggaacatag gtgttcgtcc accggtcatc
    19441 atctgatgga attcgtccaa acctgcgtac cctgttgctg aaagccaagc aaggatcgct
    19501 gtcagccacc aaatttttag cttggggaaa acacctaaaa acaagctgcc tcccaaaata
    19561 aaataggtac cgaaatgtgc gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc
    19621 agatggttga tactcaccat acttccacca taattaaaac gtatacccgc taatgtgtct
    19681 ttaaatggct gattaggcag ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa
    19741 gtttgagaag aactgtaaaa taagattata acgactatca gtgcgattac taaataaata
    19801 tttccgtttt tcatttgttt ttttgaagaa ttcataagtc acctctgtat tttatcttag
    19861 actttttttg aagaaacaac aatcattttc atagagactt cttccataaa aaagtgctat
    19921 acttaatttt gtattacaat atgaagggag ttttttcatg agttattcat taaattggaa
    19981 tctagattct attttttccg gaggcagtca ttctgatgct ttgaaccaac ggatgaagca
    20041 attggaagac caaatgaacg agtattatca ccgcgtaaca aaatggtcac ctagttctga
    20101 caatgcagag cagttaaacg ctattcttca attgcaagaa acgattacga atggttttac
    20161 ccaatgcagc agctatatta ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa
    20221 ggtcttatca ggcaagcttt acgcaatgct gcctcgtctg caatcagccg aaactgtatt
    20281 gtcaaagaaa tttgcagaaa tatctgacaa tgattggaat cagatgcttt ctcaatcttc
    20341 gtttgaaaca attgcttttc gtttaaacga gattcgtcgt gatggctcac aattattatc
    20401 agaagctgaa gaaaacatta taaatacgct ttctttagat ggtctaaatg cttggagcag
    20461 tcattatgat acgattgtag ccagcatttc gattcccttc gaacaagatg gacaagtagt
    20521 cgaattatct gctggacaag ccttcaataa gatgatgggc gatccagatc caaaagtcag
    20581 agaaacactt ttcgcagcat gggaagaagc ctggaaagaa aaggcacccc tcttcacgga
    20641 tactttgaat catttagatg gttttagact ttctgactat gaacttcatg gtgtcacaga
    20701 ttttctacaa aaaccattag agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg
    20761 ggatacaatc cagaaaaaca aacaacccct tgttaattac ttgactagaa aagctaatct
    20821 tttcggtaaa gaaaaaatgg aatggcaaga tcaagatgca cctattattt taggcgattt
    20881 gaaagaaaaa acctttagtt ttgacgaagc agcagcattt attatcgaaa actttaacaa
    20941 attcagtcca aaaatggcta aatttgccca atctgctttt gaaaagagct ggatcgaagc
    21001 agaagatcgt cctgggaaac gtcctggtgg ttattgtact gagttaccag aaacaaaaga
    21061 atcaaggatc tttatgacct acagtaattc tgttaatgag gttgcgacac ttgctcatga
    21121 actaggacat gctttccata gcagcacgat gtgggattta ccttctttaa atcgcgaata
    21181 tgccatgaat gtcgcagaaa cagcaagtac attcgcagaa ttgatcgtag ccgatgctac
    21241 attaaaagtc gcaaaaacaa aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa
    21301 tgctttagct atgttcatga atatccattc ccgattcatt tttgaaaatc gattctatga
    21361 agcacggcaa gaaggattag tctcagaaga taagatcaca gagatgatgg ttgaggcgca
    21421 aaaagaaggc tatcaaggag ctttagctac ttaccatcca tatttctggg ctgcaaaatt
    21481 gcatttcttt attgatgacg taccgtttta taacttccca tacacatttg gctacctctt
    21541 tagtttgggc atctatgctt acgccaacca aaagggagca aattttgaag aagaatatat
    21601 ccatttacta cgcgatactg cctcaatgac gacggaagat cttgcgaaga aacatttgaa
    21661 tgtcgatctt acccaaccag atttttggca agcaggtatc gacatggtga ctgaagatat
    21721 ccaaacgttc atggaattga cagaagaatt tatctgattt ctattttatc acaataaaaa
    21781 aagagttagt ccagaatggg ctaactcttt ttg
//
LOCUS       DANJMI010000048        20945 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000048 DANJMI010000000
VERSION     DANJMI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.048"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            591..1280
                     /locus_tag="PWU81_002170"
     CDS             591..1280
                     /locus_tag="PWU81_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1110858.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="PWU81_002171"
     CDS             1267..2475
                     /locus_tag="PWU81_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL1110859.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="PWU81_002172"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="PWU81_002172"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL1110860.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="PWU81_002173"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="PWU81_002173"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL1110861.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="PWU81_002174"
     CDS             complement(6578..7273)
                     /locus_tag="PWU81_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HDL1110862.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="PWU81_002175"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="PWU81_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL1110863.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="PWU81_002176"
     CDS             complement(8472..8663)
                     /locus_tag="PWU81_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDL1110864.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="PWU81_002177"
     CDS             complement(8684..9574)
                     /locus_tag="PWU81_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDL1110865.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="PWU81_002178"
     CDS             complement(9561..10328)
                     /locus_tag="PWU81_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL1110866.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="PWU81_002179"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="PWU81_002179"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL1110867.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="PWU81_002180"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="PWU81_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL1110868.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="PWU81_002181"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="PWU81_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL1110869.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="PWU81_002182"
     CDS             14899..15282
                     /locus_tag="PWU81_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110870.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="PWU81_002183"
     CDS             complement(15450..16208)
                     /locus_tag="PWU81_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HDL1110871.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="PWU81_002184"
     CDS             complement(16226..17044)
                     /locus_tag="PWU81_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL1110872.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="PWU81_002185"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="PWU81_002185"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL1110873.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="PWU81_002186"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="PWU81_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL1110874.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="PWU81_002187"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="PWU81_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL1110875.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="PWU81_002188"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="PWU81_002188"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL1110876.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5988 a         4261 c         3838 g         6858 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaa
//
LOCUS       DANJMI010000049        20877 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000049 DANJMI010000000
VERSION     DANJMI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.049"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(47..1630)
                     /locus_tag="PWU81_002189"
     CDS             complement(47..1630)
                     /locus_tag="PWU81_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110877.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDH
                     LRKEINSEKELLNYVHKIK"
     gene            1752..2336
                     /locus_tag="PWU81_002190"
     CDS             1752..2336
                     /locus_tag="PWU81_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110878.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            2347..2592
                     /locus_tag="PWU81_002191"
     CDS             2347..2592
                     /locus_tag="PWU81_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110879.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            2598..3320
                     /locus_tag="PWU81_002192"
     CDS             2598..3320
                     /locus_tag="PWU81_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110880.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            3451..4194
                     /locus_tag="PWU81_002193"
     CDS             3451..4194
                     /locus_tag="PWU81_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110881.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            4200..4565
                     /locus_tag="PWU81_002194"
     CDS             4200..4565
                     /locus_tag="PWU81_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110882.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            4581..5111
                     /locus_tag="PWU81_002195"
     CDS             4581..5111
                     /locus_tag="PWU81_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110883.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            5273..5581
                     /locus_tag="PWU81_002196"
     CDS             5273..5581
                     /locus_tag="PWU81_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110884.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            5577..5996
                     /locus_tag="PWU81_002197"
                     /pseudo
     CDS             5577..5996
                     /locus_tag="PWU81_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6133..6933
                     /locus_tag="PWU81_002198"
     CDS             6133..6933
                     /locus_tag="PWU81_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110885.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            6970..7152
                     /locus_tag="PWU81_002199"
     CDS             6970..7152
                     /locus_tag="PWU81_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110886.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            7263..7580
                     /locus_tag="PWU81_002200"
     CDS             7263..7580
                     /locus_tag="PWU81_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110887.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            7583..7815
                     /locus_tag="PWU81_002201"
                     /pseudo
     CDS             7583..7815
                     /locus_tag="PWU81_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7930..8409)
                     /locus_tag="PWU81_002202"
     CDS             complement(7930..8409)
                     /locus_tag="PWU81_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110888.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            8529..9866
                     /locus_tag="PWU81_002203"
     CDS             8529..9866
                     /locus_tag="PWU81_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL1110889.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            9889..10416
                     /locus_tag="PWU81_002204"
     CDS             9889..10416
                     /locus_tag="PWU81_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110890.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            10403..10966
                     /locus_tag="PWU81_002205"
     CDS             10403..10966
                     /locus_tag="PWU81_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110891.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            11388..12125
                     /locus_tag="PWU81_002206"
     CDS             11388..12125
                     /locus_tag="PWU81_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110892.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(12142..12558)
                     /locus_tag="PWU81_002207"
     CDS             complement(12142..12558)
                     /locus_tag="PWU81_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110893.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            12950..14197
                     /locus_tag="PWU81_002208"
     CDS             12950..14197
                     /locus_tag="PWU81_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL1110894.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            14465..16855
                     /locus_tag="PWU81_002209"
     CDS             14465..16855
                     /locus_tag="PWU81_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HDL1110895.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            16904..18739
                     /locus_tag="PWU81_002210"
     CDS             16904..18739
                     /locus_tag="PWU81_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL1110896.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKSVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            18808..19671
                     /locus_tag="PWU81_002211"
     CDS             18808..19671
                     /locus_tag="PWU81_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL1110897.1"
                     /translation="MSMKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNS
                     YKEKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGEL
                     VGEILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVG
                     DNSHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKI
                     VAKRRGMKNEE"
     gene            19661..20770
                     /locus_tag="PWU81_002212"
     CDS             19661..20770
                     /locus_tag="PWU81_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL1110898.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
BASE COUNT         7697 a         2932 c         4052 g         6196 t
ORIGIN      
        1 tgaaagtaaa tatacgctat aaaagtgcat cttgtgttat aaaaaattat tttattttgt
       61 gaacataatt taacaattct ttctcggaat tgatttcttt tcttaaatgg tcttcagact
      121 tattaataat attatctatt atgtctagat acatatcact tttcgattca gctaattcat
      181 acagtttttt taattcttta tcctttaatt gaaaaccctg atccatagtc actgcatttg
      241 gatatagaag cccactataa tgtttacgta aattcacttg gaatttctga ttataaaagt
      301 ctaacagatt agaaataaaa ggcggaaaat tactgttttt gtaaaattga ttatttccat
      361 ttaggttgta tagtaatttg ttaaattgct cctgatttaa tttattgtaa atccatggta
      421 atacttccat atccttatct gctaacctga atgaactagc atcggtaaga tgttcgatca
      481 taaaatctat gaacatctct ttttcaccaa aatataaata ttgattatat attgtttcta
      541 atgttgaaga tggtagcgaa gaaactctca ttttttgctg gagtatagga tcaactagac
      601 tatttaagtg attttgtaat tcttcattgt tgtttcctag aactgcaagg gaacgtaaac
      661 ggtagtaata ttctatttct gttagtactt gttgtttcgg tttaaaatca tttattgata
      721 tttgtaaatt ttgaattgtc tcgtctgaaa aatcatgttg taattctgga tgggtagcta
      781 taaaagtaag taaaaagaac ccttttgtat acgtgatctg ttgattttca ccaagtgatt
      841 tattaatttc ctctcttgat tcagctgtga acctatactt tgcaatttct ttttttagaa
      901 tatttaaata gaattcttta ttatcaaaaa ttagggcatt aagtagtctg aaagtacttt
      961 ccctatctct atctgcttcc tcatttccat aagataattg aaaaacaaac ttaaaggctg
     1021 tcttacatag gttttctttg gctttttcgc ttagtttatt tagatagcta ttttttagtg
     1081 cagagtgaaa ttcttcaggt gtaacagaca tagcatgtat gcgacttgca tattgtataa
     1141 tttcgatcgt tacttttttt cctaatattg gattttttag gaatataatt tcataggact
     1201 tccgtataac atcctgacaa gtttcttttg ttatatctct taattcccaa gtattaccgt
     1261 tgtaagtgac tacaggatga gctgaataat ttctttgatc cttaatatat tttaaatcct
     1321 catattcata gctatcaatt agctcagttg aactataagc tttttctagt agttccattt
     1381 cccattttgg agagtttgga tcactttttc ttagtctagc tatatcttct aaaattttct
     1441 tagaagtatt atcctcgtaa atatttgcta aatcagataa tttatcaatt aaatctaaaa
     1501 taagcgcact gtaattgaca gctattgcag aacgataatt tccctgataa aaactacttc
     1561 taacttcttc taataatgga ataattctag gatttttaac tttttttatt tcatcatcta
     1621 atataaacat ataaatctcc tttttttatt tgtatgacaa gtttaccata aaaaaaaatt
     1681 aaaaagcgag acatatttat gttgttgtgt tatcataata acataaatat gtagagtgag
     1741 atgatgaaaa aatggatttg aaacaatcgt tttacatctg gttatatcct gataatccta
     1801 tgtcaataat ggctgatatt tttgtagtta gcatcttatt gtatttattt tatctaattt
     1861 tctttcacaa tttaatcggg tacatgattc ttgattggaa aggaatgttc gatatagaaa
     1921 gaaaaccatt tgctaacaat caaacctata ccgtcttcga aagtggagat tgggaagaaa
     1981 atgaagttga tgtgatccat gaaattggat tagctttatc aggaatacaa gatgctgatg
     2041 ttttggtatc aatgacaacg gatactggat acaacgctta tgaggtattt tatcttatgt
     2101 attttatatg gcgtgatggt caagaaatta tcaatggtcg tgctgaaaaa acaatcaatt
     2161 ctaatactga aatttctttt tgtatgggtg aaggggaaat gattgtatat aaagatgggt
     2221 cagcaaacat taaaacagcc tttggtgata ttgaaacaag tattaaggaa cttagggaat
     2281 atgtgcagaa cccatataat tttcaatatt tagcaggaag aataaattta gtttaggagt
     2341 aaaaatatga aaaaaattat aaatcaacca acaaatcact tgataattga aggtgttatt
     2401 ttcgtaacaa tgtattttac tgtaggaaat ggcatctcaa atacaatcat tcaaaaaaat
     2461 ccacaatacc tattatttag tctttatgct tggattgtac aaatggtttg ggtttccata
     2521 ttgatgctgt tgttttattg gatagtacgg gttattcttt tattgttaaa caaaagaaaa
     2581 aaggataggt gaataaaatg aaatcaacta aattgaaggt tgtattgtta tcgacagcag
     2641 cattaataac ccttacagca tgtggtacaa ctaatagtgc taataataac aaagacacaa
     2701 agtctagtag tacgatggtt actaaaaaca gcactacatc ggcatcaaca acaatgtctt
     2761 caatgactga aaataatgct gaaaagtatc aaaaatacga agacattaat aaaggtcaaa
     2821 agccggatga agtttataac aactttatta atacagtaaa aaatgatgaa aattatggga
     2881 atgatccatt gcaaaataaa aatctgacta tcgttcttta caaacctgat tgtccagatt
     2941 gtcaaaaagc agaatcaaaa atcatgcaag aaataaaaga aggatttagt tatgctccaa
     3001 cattaatgca tgatattacc ataaataaac cagcttacga gtattacgca gttgctgtag
     3061 atattactga tggtgtacct gagtgggtac aaaacattcc tggtctggct gatgagaaca
     3121 atcaatatca tacgccaaca gttgcagtag taacaccaaa gaaagttaac ggcacactat
     3181 actgggatgt aatgggaatg acaacaaata caactgatga aatgattgaa agtacaacta
     3241 aatattatat ggatttggga gcatataaat tatatcaaca atcagagcct gttatggacg
     3301 atcaatccaa agttaaatag atctttaggg cgtaagccct atttttttaa aattaagtga
     3361 taacaaataa atgatttctg aaacaaacgt tcttactcaa ctactcgaaa cacacaagct
     3421 aattaactag attataaagg agaatgcgta atgaaagaaa tcagtgcaaa tgaagctcat
     3481 attccgcaaa agaatcaggt tggtatatat aaggtgcttc atggtgaagg aatttataaa
     3541 atacagttac ttaccggtta caataatggt gcaaatggga agtggttaaa ggcaacaagt
     3601 attcgtgaac ttagaagtga agtagaaaaa attaggcttg gtctcacaag tgaagaaata
     3661 gctaaactga agggaaataa ttcttttgta attgaaaaag aaaaggaaaa acatctactt
     3721 tatcgtgagc tgaaaattga agaatactat gaaattgatt tacctgaaaa attaagaatt
     3781 attgatcaac gttcagaaga aaaatggaaa actgaaatat atccaagctt aaatgactac
     3841 aagacatcag gaaagcccat gtataactac acaggctttt taacctacac acaatacaaa
     3901 tatgcctttc gcaaattaaa aggcattaaa caagctgaaa ttgccctgta tgagatgaaa
     3961 aaagtggata gattgaaatt gattcaagat ttagtgcaaa atacatggaa tcaagcgatt
     4021 gtcgagctag aaaaagaaaa tgggaaaatt ttaactaata aatatctgat cgtaaatgaa
     4081 attgaagaat taattggttt attttcatgt gaaaaaggtg aagtcttctt gaagtcatca
     4141 ccaacctatt caattttgga cagtccgtat cagtttatac aggcaaggag atgagttaga
     4201 tgtctagctt gaaagatgac tatatccaag caaaacaaga gtatgaaaaa atacagatgt
     4261 tgaaatctga gaaagtcgaa ttatttttaa cacaaaaatt agcagaggat catattgcta
     4321 acttagatgc taaaacttta gaggaatatg atcggcagtt taatcagata catcacaata
     4381 tcattttgct tgttgagcag gcaaagcgaa ggtttgaaaa agcggaatat gatctattag
     4441 atcaaggaat tgtatgcttg gataagggat tgcgaagata ttatggtgca gagttaaaaa
     4501 agcaaaatgt agatagaaat gcactaaaac atgaaatctt agaattgata atgcaacgaa
     4561 tttgaaggga tagaaagcaa atgtcacaaa acattagtgc tgtaaaacaa ttaatagaag
     4621 aagatttaga gaccacagtt cagagattag cggaacgaat ccattttgca aattatcaaa
     4681 ccatctctaa ctggtcaagc aggaacagaa atccggaaga aattccaatg aaattttttt
     4741 atcgaatcgg aaaagaatat ggtgttgatc cgataaaaat tttcgagaag accatgattt
     4801 attactatca tggaacacct aacgaagtgt tagaacatct tttggaagag gcaaaagaaa
     4861 aatcatcaat attagagcta tcgcaaaatg acaaagttat tcaagtgaaa tgcgttgatc
     4921 caacaggaaa gattattgtc ggttttgata ttgaaagaaa atccaaaata gattttatta
     4981 cacaattaaa aaagataaat aatttagata ttctaacgct tagaccagat catgaacagt
     5041 tgtttatgag ttttaaaaaa caattgaaag aaatacaaaa aagatataac aaggtgtttc
     5101 ctaatgcgtg aaaagctcaa agaaatcaga gttttttgga taattaacaa agaatgtatc
     5161 atcttatata ctcttggatt tcttagcatg atcttgtttt ggtggttata tttaaaacag
     5221 taattggaga gggtcaagag gatttttatc aagataagga tgtggaaact gcatgtggaa
     5281 taatagatta aaaacaggat tgttattaat agttatttcc tgcgctatga tgattggtat
     5341 gaggatacaa agggaacaat cctattttga agtttctgct aacaacgtaa tagaaaaatg
     5401 ctattacggt caacattatt ggagtgagga agtcagagaa aatatagatc gtgaatatgt
     5461 tcaaagaatt gtttgggatg catattctat caaagattat cctaaaagtt taacgagtag
     5521 attattttac agtgaaaaag ataatcaaaa actctcagat ttgatgatga aaaaagtatg
     5581 actattatta cgaaagagaa acagagtgct atattcgagg ggaaaaagcc tacaacaaaa
     5641 gtgactattg gggtgacaag gcgaagcgat taactgcaaa ttttagagtg tatgaatcag
     5701 agtttataaa cttgaccttt cttaatagtg tttttattga ttatgtaatt actacgaaac
     5761 gcattggaag ttattcaagt acctacgcat ccttagttcc aatgttaaac tcgatgagcc
     5821 gtttcttaaa agaaagagaa aagaaagagc tacagtatat caaagaaatc atgccggatt
     5881 ttgaaatgca cgaatggcaa attgctttat cagattggaa attggcgaat aaggttcgta
     5941 gaataacacc ttttcaagcc aagcgctttt tgagagctat gaaatttgtg caataaaaaa
     6001 agccaccaga caagttgatg gagactaaca caattaccaa cagttgtaat tgtgttagct
     6061 tacaaattaa ttatggtgtt tagtcaatcc caaattgtga aatggcacca ataattggaa
     6121 aggaaaaatg aaatgtctga agatttacga gagctgaagt tacgaaagaa gcagtatgat
     6181 cagtattttt ccggtttgtt gaataaacat attgaagaag gacttgatca tgattttgga
     6241 aaagatgaat attgtttata ttgcgaacgt tacaatgaaa aaatgtctga atatctacag
     6301 ctgaaaaaag agatacagaa attagagaaa aaagtacatg cttggaagta taaagattcc
     6361 atgtctggtt atgttgaatc tcaaaataag ccaagaaaaa agaagcaagc gtatgttcta
     6421 aagatggtaa atggggaaga gttaattgta ttagcgtaca gtgaagaaga ggttcgagaa
     6481 ctcgttgaaa cacctgtcag gaaaaaggtt gaaacaataa atgagataga cgacagccaa
     6541 tacaatagca tctatattga aacaagtcat ggtggatata agaaattagc aactttcctt
     6601 agaagttcta aagttgggat aatttctaaa aaagacataa gtgctgttac cgatagtttt
     6661 tgtactttat ctgttcctaa agcttatttc caatacgata gtaaaagatt tgaacgctta
     6721 gagaaattct gtagaccgta tactgtattg aaaactaaaa aatttaataa aactaaaaga
     6781 gtttctatac agttaccgac tggtcaaatc tcattaggtg aaaaagacta tctgataaaa
     6841 gtaggaaaag agttaattat aaaagtaccg gaacacttat ttaaagaaaa ttttgagata
     6901 cgtgaaacct tggtatggaa agaagaaaaa tagtttggaa gacatagaat ggtataaggg
     6961 gaaattgaaa tggcagtaaa taaaatattg caggtactaa tcatagacga atttggcggg
     7021 ggagaatggt ttgatgtgca gtccatagac tttgaagaag aagtcgtagt tatagaaaat
     7081 atttacggcg aagtatattc atattcgctt gaaggtgaga atattgagga tttacggttg
     7141 attggtgagt agaaaaaaac agaaatatca aaatggagat aaaaattaat agcatgtaaa
     7201 gatagtcatg cttgggttaa aaatgttaaa attcaaaaaa atgagtcaga agggataaaa
     7261 gtatgaaaag agaggaatac aaacaaagat taaacgaact gcttgaagag gacgaaacat
     7321 taactcatgg ttcgcctgat gaaattctat acatgattga taatatggtg atctttggtg
     7381 gatatgaact tggtaatagg tcagttgatc ataatatttt agaatttgat gatgtatcat
     7441 gggaagaaat tcttgattgg ggaattttgg cggtacctga aactaaaacc tatattagtg
     7501 acactatggt tccttttttt gaggaattgg attataaaag acttcccaaa aatgaaaatc
     7561 atatactggg aggaaactaa caatgttaag tgacttattt attttactga tctatctagg
     7621 tgtaagtgaa attattgcgt attttttgtt tgtcattgct aattatggta tttccagtgc
     7681 aatcgttcaa atgtttagat taccagtaag tgaactaaaa aaatgatgag attatcagca
     7741 tatcttttaa tgtttttgaa tgtggtcacg ttaatctttg tagtaatcgg tgtgactttc
     7801 gatttaatgg aatagtggga aatctataat ggaaaaaaga gacacgaatg aacatatttt
     7861 ttgaatcaat attgttgatg gtttttgcga tcagcgctta ccagaaatct aaaaaagagt
     7921 aatgattgtt taatcatcac tctttttttg tagggaaatt gaaatatgtg gagcttgtat
     7981 tggtttagtt aggattgctt ggctataagg agctagttct ttatcaaaac catcaaagta
     8041 ggttttcagt agcgatttaa ctgatcgctc atcttgtttg atttggataa tttcagcatc
     8101 caaatcggtt gtgagatcag gagcttttga tgcgattacc tgattttttt tatcttccaa
     8161 ctctcttagg ctgtttaaca agttgtaaaa agaatcattt tgattgttca ttccaacgtg
     8221 agttgcaatt ttaacagaag tttcgtccgt attgtcatca aaagaactca ttggaatatc
     8281 aagtagtaac gaggatgttt cttttactga ctttttcttt acagcatttt ttgtttttcc
     8341 atcttttttg ctctttactc gagatgtttt tggagctagt ttaggtaaat cacttgtttt
     8401 atcagtcatt tattagcttt tcctttctat taatttatgt tattatatta tcataataac
     8461 ataaaaaagt acactttttg ggggcgtagt tattgaaaaa atctaagaga aagattgcca
     8521 cattgcttat gttaatggtg caatttacag ggattgcaat gagtgtaatc acaataaatc
     8581 cgcaagtgat acatgctgat gggatagcta cttatgatgc acaatttaac tgtactactg
     8641 aaggtagtgg gtcaggaaca gatagcgatg gaaacgatac tggaagtggc gctgtaacag
     8701 gtgatgtagc caaaaatatt gaagatattt acaaatacat gaatggagaa tatggcttat
     8761 caggtgctag tattgcaggt attttaggta actggaaaca agaatcaggt attgatcctc
     8821 gagcagtaga agggtattac ggagatggac agagagaagt tgacttcatc aaacaattta
     8881 ctgatggtct gatgggtatt ggctatggtc aatggacagc tgggcgacat acagcattag
     8941 ttgattggtc taagaaaaat aatggcggtt cttggtggac tacagaagcc caattggact
     9001 ttatgttcaa aggtgatggg ggattcgtag gaatattaaa agattatgct ttaaatgctt
     9061 ctgatgatgt cgtagaaaat gcagtaactt ttcataaaaa ctgggaaatt tcagctgatt
     9121 ccgaatcagc tgtaatagct gtacgtggtg aaaatgcaaa agaaatctgg aagtacatga
     9181 aaagcaaggg tatggacggt gcaaaagatg aaagtaagat caataaaata tccggaggtt
     9241 caagtaccgg agatggatcg aataattcat caagttcagc atcaggaaca gtagaagatg
     9301 cctgtgcaac tacggatgac gctaattctg gttctggatc aattgggaaa gaaggaaaaa
     9361 ttaacggtaa aaaaggtcaa gtaatagcga atatgactta tgaagaagct ttgaaaaaat
     9421 atgggaaagc gatcacgcta ccaaaattca aagaaccaga ttggtcaact tcacctttta
     9481 acggaatata tcaaggtcag tgtacagagt tgacgtgggc ttatatgagc caattatacg
     9541 atgggaatca accgactact ggaaatggtg gttatgtatg ggaaagctac aaatcggcag
     9601 gagcaaaaat aacgaacaaa ccaatcgtag gttatgggtt tagtgcttcg gatggatggt
     9661 tatgggctgt tggagatgca gggcatactg gtgtagttat agcggtgttt gaagatggat
     9721 catttctatg tgctaacttt aatgttccac caacccaagc tccaacaagg ggagtttcgg
     9781 taacgttgat tgatggagtg gacggatcag ataatgtgca tttctttagc ggtgtaggaa
     9841 aagctaaatt tgaaatcgaa aaataaattg gaatgaagga cgatatagat gaatagtaaa
     9901 gtgaaagata caattatcat ttctttgctt tctggtatgt tattagcact gcttgttgga
     9961 atcctattct tcaataagca gaatcaggac atagaagctg aaaaacacag tgtgcatatt
    10021 gaagataagc tgacattctt taaagaggtt aaaccaaatg aggtggatgg attacggaaa
    10081 gatttagaaa atgatattca agaaatgttg aatggatcag ttcaaaataa gtttgcagat
    10141 caatcaggtg aagaattttt gagaaaacta tttattgtaa caggtaaacc accaattact
    10201 gataagagta gtgagaaaga aaaaatcgag tactataagc cttttacttt taagctaaca
    10261 aactttggaa ttaagacatc tagtagtggg ttgtatgaag cattgaccga ttttgaagtt
    10321 caatacaatg gaaaaaaagt gtatgatacc acattaacca tcacactaaa taatgataag
    10381 aaagtgactg ggggtgcttt ttatgcaaaa tcatgaaatc actactgcta aaaaggcgat
    10441 cggaatactt tccataattg tgatcctttg ttgtgtggta ctatttcaaa agaaaaatgc
    10501 ttatgataat aatgtcaaag aattggaaga ccttaaaagt aaacaaatcg aagtagtcaa
    10561 gaataaccag aaagtgaaag ctatgcagga agctcaatac aaagaattgg gaaatgctga
    10621 aattaaggaa caaagtagga aattctttaa tttattttac aactggaatt catgggaaaa
    10681 atatacgaag aatatgaagg aaatacaaaa agaatttcct cagatagtaa agtctagtcc
    10741 tgttgatatt tctggcacga tggtaggcac tggtaatagt ccaatatcta gctatactgc
    10801 tgattactat acaacaaatc aaaaaggtaa aatggttgaa ttgattacac agacaagaaa
    10861 tactccaaca acagcaacaa caacaatttg gcgtggagta acaagcatag gagaaaatca
    10921 attgttttat ttggaggatt tagatagcta tgtaagagca gagtaaaata aaaaaagagc
    10981 taacggtacc aacgttagct ctttgtgctg acgatgataa caaaacctac cgaaaaagga
    11041 ttgtttcatc acaaacacaa acagaaaaga attgataaaa aacccgaacc tcagaatttt
    11101 tattaatcaa ttcatttaga ttataataaa ttgagctgaa cattacaata cgtatatgtt
    11161 gtaaaacgta aaaaaagtca aaagtaaata ctttcttaaa aagtcaaata aaactattaa
    11221 agacaatata tatggtttat ttgacataag aaaatggtct agatgataat tgtaatatag
    11281 aattgtagac ttaaataaag cataaaatac cttttatgat aaggtatagc tcttaatttt
    11341 aagcagtttt agatataaga ttgaaagtaa agtgggggag aataataatg gcagatcata
    11401 aattagaaaa gttattagaa gaaacgtgga atccaaaaga atttagtgaa ttttttatgg
    11461 agaattttga aactgactta gcagtgattg ttaaagatgc tcttagagag caagggtatc
    11521 ctgaaactgc aaactatata aacattaatt ttactttgta tactgaaaat aaaggaactt
    11581 gggatttttg ggctacttta gcaaataagg agttaagtga taaatcagac acaggaattc
    11641 gtaacttttt tgaatcaaac agagatgatt atatgtatgc taacaatcaa aataaattga
    11701 actttagagt agagtttgat gaaacacctg aagaatttat agaaagacaa ccgcctaaag
    11761 aaaatgtagc aaaggtattg gaagatcgtt ggaattcaga tgaaatagtt tcaacaatta
    11821 gcgaactagg cggacaatat gaaccgctag tagaagcggt tagagaagag ctaagactga
    11881 ataagtttcc tgatgttcaa aatattgatg tttctcaaat tgaaataaac gtcaagatca
    11941 cgaataaact tgattatggt agttgggcag atatagcatt agaaaaatac atctactcaa
    12001 cgttaaaaga gtttatcgaa aatagaatgg atatcatgta tctacagcat ccacagtatt
    12061 taaactttgg tgttgaaata gctacacctc ttgaagaatg gaaaatggag caagggcttg
    12121 actagccttt gctccatttt tttattttac aaatgaatac agttcgtcgt ttttaccaag
    12181 aatgattgtt tgcccaactt tgagattgtc aggtgatcct tcagcggttt gtaaggagaa
    12241 cgttccattg aacttatcgg ataccgttat aggtagcttt aattctgatc cttcctcatc
    12301 tttgagaatt gctgtttttc gttcatcaaa ataaacagtc tttattaaat aatttttcgt
    12361 agtgatctca gcagatttgt ctttttttgt agtagtacca gtttctttag aattagaggt
    12421 attttctacg actattccat ctttggcatt tgagcttgtg tttatttgat tgaatatttt
    12481 cagcgacaag ataacgctaa gaatagtcaa tatagaaaca agtgtaatta atactgataa
    12541 tttccttttt ttgttcattt tttcttaaca tccttttttg ttttgattag ttagatattc
    12601 cttatctaac aagccttttt aggctattaa atgaagataa accccaaaaa atattgcaat
    12661 tatgacagtg caatggtact attattatac aataaatttt gcgaaagggg taggtctaaa
    12721 tcgcaaacaa aaagaaattg aacctagaca aatacaactt gtataccgca agggaagcaa
    12781 gtttgatttt gggtaaaggt agaaattatg ttacaaacat aatcaactat tatccaaatc
    12841 gtttgcctaa aggttcgttc aagaaagtgg gtgacaaaac ctatattata acggaagaag
    12901 gaatagaagc attgcgatca caaacagaaa ataaaaacaa aaagaaggaa tgaatatgaa
    12961 gagaaattcg cttggtatgg ttttattggc atcagctgta cttgcggggt tatcagtacc
    13021 agtaacctca catgctgatg aatggactgc aaacacaccg gatcaaatcc aaattacaac
    13081 aggacaatca agttatgcgt tcaaaaaagg agatactatt tggtcaactg tagagaagct
    13141 taaaagctta ggttacaaga ctactgttgc tgagtttgcc gaattgaaca atgttgattt
    13201 ggcaaaagga gaacaacgga ctatcccgat tggacgaact ttcaacatca gtaaagatac
    13261 attaacagtt aaggaagcag atggcgaagt agttagtcaa actgttcaaa aagataaacc
    13321 tgatcctatt gaaaatacgg agcaagagaa caaaaaatcc gagactacaa aagaatcgga
    13381 accaacagca aacacttctg ataagacaga aacaagtgct tcaagtgttg atggagcaag
    13441 ttcatctaat acaaataatt ctacaagtga gaaagaagca tcacagggaa caagtgaaag
    13501 tagcactacg gaagctgata ctggaacatc aacaaataac tcacaatcaa catcaaatga
    13561 tgcaaatgaa tcaaccagta catcttctag tgatcaatca caaacaggtt cgagtgatga
    13621 aactgacagt tctacttcac caagtaaacc cgaacaacca actaatgttg tgctaggcgt
    13681 tccatttatt agtcaaggta acacaatgct ttgcgaagga acatctttgc tagaagcgtt
    13741 acattataaa ggtgttgcaa caaatcaaga tttaatgact tttgttaaat caatgccact
    13801 atctcctgac aataatccaa ataatggttt ttctggtgaa tggagacaca atgtggatgg
    13861 aacttatcaa ggtatgaatc cggctcctgt agtttcatgg ggtgttgcta atggcggaaa
    13921 tgttgtagat atttctggtt catcggtaca aggattaaaa gatgagattg taaatggaaa
    13981 tcctgtagta gcgtgggtaa cttatgcctt tgaacctgct caacataaac caatgttttg
    14041 gggcgatgct atttggaatc gacatgtagt agccgttgat ggatttaaag acggttttta
    14101 ccatgttgtc gatcctgtgt ttggggcaag ttggattaat gcaggatcat ttgaaacagc
    14161 atataacgta tctcaattag cggtttcggt acgttaataa aagatatcgt ttatatacta
    14221 agtagttata tagtctttat ttgtccttgc ttaactactt agaaaacttg ttgctcatgg
    14281 tgggtagaac taaaaggggt gatgctatga atactagagc gttgaaacag cttgctttca
    14341 acgaaaaaaa tgattaatag gtgtggtgat tttaacttag gaatgggcgc gaaggtcatt
    14401 accacaccga catatcgtgg ctcattttct tcgcctaact ggtataaaga aggagtaacg
    14461 agagatgaag catgaaaaaa cgataatttt aaaatcaaaa ataatttatt gttttgcgac
    14521 tttagtaatg atgttatcta cgcttataat gcctttttca gcaataagag ctgaagcaga
    14581 tgtcacaaaa ccgacaagtg caaaatttat agaaactggc gtaaaaacag acggttatat
    14641 tagggttaat atgccgaatc atccaaatga atggatgatt tcaagtcaat ttaaagattc
    14701 tcatggaaat attggatatt gtatggattc agaattgcca tccccaacag gaagtggagc
    14761 cggaagcttg aagtacaagg gagctggcag tgatgagttc tatcgtatgt ttaaaggtgg
    14821 ttttccatca aaaacagcaa aggaactggg tgcaggaaat gatacagaag catggtatgc
    14881 aacacaatta gtttcatggg ttttagccgg aaactttaaa gtttcacaga ttgtatggtc
    14941 acatccaaac cataccgccg cagaaacagc tcgtgtaaaa aaagcatttg aaaaaattta
    15001 tgactatgct aagaatggaa aagatacgcc caacactgag ttttctatca cagcttctaa
    15061 gacagctgac gaaggaaaat atcacacttt cacatataaa acagcatcaa ataagactgg
    15121 taatgcgaag ctcactttta catcagcaaa accagctggt atgaagattt atgatgcaga
    15181 tggtaaagaa attactaaca acactgtgaa attaaattca agttttacta ttaaagtacc
    15241 tgtgactaca ccaagtggta ctttgtcatt taaaggtact gctaatgtta gcacaaccaa
    15301 tccttttact tttgatggac gaggtgtgta tcaagatgcc gtagttatga tcactacttc
    15361 agaaacaaag gactctaaat ctttgagtgc taaatggaca agagctgttg gtcaggtaac
    15421 agttaagaaa gttgatcaag atggaaatgc tttaaaagga gcggagttta ctctaactga
    15481 caataacaat gctaaatctg ttaagacaac cggagatgat gggttagcta cgtttgattt
    15541 gcaacaaggc ttaacataca gcttagaaga aacaaaagtt cctgaaggtt atcaaggttc
    15601 atttaaaaag gatggtatta ctgttaatac cgatggacaa gaatttagtt acactgctac
    15661 gaacacaatt aaaaaaggta aagtaaaagt taaaaaggtt gaccaagatg gaaaaccatt
    15721 agctaatgct gaatttacct tgacggatga ttcatcccaa acacaagcag aaaaaacaga
    15781 tgaaaacgga cttgcaagct tcgatattgt agcaaacaag acctacagcc ttgaagagac
    15841 cggagtacca actggttata caggcgtatt caagcaagaa ggaattaccg ttaaagatga
    15901 tggacaaact tttgaatata cagctacaaa cactttgaaa aaaggtttag taaaagttca
    15961 taaagtcgat caagatggaa aacctttggc aaatgcagag ttcactttaa ccgatgaaaa
    16021 aggtgcaaca caagtagaaa aaacagatga aaatggactt gcaagcttca atattgtagc
    16081 aaacaagacg tatagcctag aagaaaccgg agtaccagta ggttacacag gtgcatttaa
    16141 gcaagagggt atcaccgtta aagacgatgg gcaaactttt gaatatacag ctacaaataa
    16201 tttgaaaaaa ggtgtagtaa aagttcataa agtcgatcaa gatggaaaac ctttagcaga
    16261 tgcagaattc accttaacgg atgaaaaagg tgtaaaagaa gttaaaaaga cggatgcaac
    16321 tggtctggct acgttcaaca ttattgcgaa caaaacttat aacttagaag aaacaaaagt
    16381 accaacaggt tacacaggcg cattcaaaca agaaggaatt accgtcaaag atgatggaca
    16441 agtctttgaa tatactgcta aaaacacttt gaaaaaaggt gtgatcaaag ttcataaagt
    16501 ggatgatcaa gggaaagcat tagcaaacgc agaattcact ttgacagacg acaaaggtgt
    16561 aaaagttgta caaaaaacgg acagcaatgg cttggcaaca tttaatattg ttgccaacaa
    16621 aacgtacaaa atcgaagaaa cacaaaaccc taatggttat acagggaaat ggtctaaaga
    16681 aggcatcacc gttgctgacg atggacaaac ctttgaatat acagtaatta atcaccttgt
    16741 acctgaaaca gtaagaacag gcggaatcgg tacatcagcc ttccttcttg tatttctaat
    16801 tgcaggattg agtatggtgc tactgtatta tcgagaacag cacaaagcaa aataaaaaag
    16861 tgttaatgga aaagaagtaa atatgaaaaa agaaggctga cgtatgaaat caaaaaaaat
    16921 tttttcaaat ttaatgctat ttggtacttt gctaggagtg ggattgggcg caagctcaat
    16981 ctcttctagc cttggactag gggggcaagt agcatatgca caacaaaact ataattatca
    17041 aggaactgga acgattcata tccataaagt aacaaatact aatggggatg ggaaccctgt
    17101 ctcagatatt gataacaaag ggaaagaaat agctttacca aatggtgtaa aacctcttga
    17161 aggcgttgag ttctcttatg tgaaacttac taaagatgaa gctaaatcct tgggatggac
    17221 ggatagtgat actacaaaag gtcaacatat tgatgatgct aaagcacgat tattactaaa
    17281 caaaaaatac agtgaagtaa gtagaatgaa gactgattca caagggaata tagacatacc
    17341 tatacaagta aatacagacg atttaacaga taacttgtat ttgattgttg aaacaggaac
    17401 agtcaaaggt gtagttgaag aagcgtatcc tatgattgtt aacgtacctg aactaaacgg
    17461 atcagatgat caatacttta attatgatgt acatgtctat cctaaaaact atttccaatt
    17521 aaactcagca gagcttgata aaaaagacga agatggaaag gctcttgccg gaactgtttt
    17581 tgaattgcga aaagatgatg gatctgatca ggttgttgga acttataaaa cagacagcac
    17641 aggtaaatta actgtagatg gtttggaatt aggtgactat tatttcctag aaaccgattt
    17701 gggaacagat gctaataaaa caacctattt actagatggt aaaaaattca aatttagcgt
    17761 taaacgtggg gagactgcta aagtagttct tgatgttacc aactacaaac gacctgtaat
    17821 tactaaggta attgataaag aaaccgttga tactggcgaa gatgttcaat atacattgaa
    17881 accatcaatt cctggtaata ttggtaatta cgaaaaatat gttgtcaaag atgttctagg
    17941 tgcagagtta acttatgatt catcagaagt gatcgaagga atgactttag atactgatta
    18001 cagtatcgaa cgtgacggac aaacgacaaa ctatgtcttt actgaatcag gtatggataa
    18061 gcttcagaaa caatttgatg caggggatcg cttctttaca attttgatga acgctcatac
    18121 taatagtaaa gctaaaatgg atacaccact taagaatgat gtttcaacga tctacaacaa
    18181 tggatatgaa cccgaagacg aaacaccagt tgatcacgct tatacaatca ttggtgatca
    18241 ccaattcatt aaaacagatg gagatacaag caaagcattg agtggagcaa aatttgtagt
    18301 gaagaacaaa attgatccga ataaagttac ttacctaaaa caagatccaa caacgaaatc
    18361 tgttacttgg attgcagata aatctaaagc tactgaattc gtttccggat cagatggtaa
    18421 gttccaaatt gaaggcttgc aatataccta tgatgtagat tttgataaac aaggtaatcc
    18481 aacaatcaca gataaaaaaa tcaatgatta tgctcttgaa gaagttgttg ctccttcagg
    18541 atatacacca actagaaatg atgttccatt tgtggtatcg aatgatgatg cttctagcga
    18601 agtaacagac atcaagaacc ttaaaacacc tgaaacagtt cgtacaggtg gaattggaac
    18661 agtagtattt ctagtggttg caggtgtggc aggaacaggg ctatatgttg tctacaaacg
    18721 agaacaacac aaagcttaat gaaataaaat tccctagagt gtttttggct ctagggtttc
    18781 tttttaaaaa gtgtgtgaaa agtaggtatg agcatgaaaa agaagaatat caagttgatc
    18841 atcttaaaaa ttgggatgtt gctatgttta ggagtaggtc tatatcctgt aattactcag
    18901 gcttatacct actaccaaac tcaacaaaca ctaaactcct ataaggaaaa atatgatgaa
    18961 gcttctgaaa aggagaatga agaagtcttc aagagatatt cagaagttaa taggaaacga
    19021 aaagacacag aacaaacctc agtcacgatt cctgaagata ataacaatat tccgtcaaat
    19081 ggaaatcttc agaaggataa attaggtgaa ttagtgggtg aaattcttat accaaagatt
    19141 aatgtggatt tgccaattta tgatggtgta aacgatttcc aactatcagc aggagcagga
    19201 atattaaccg gaactgatta tccaactggt gggattggaa accattctgt tatatcagct
    19261 cattcaggca ttcctaataa aaaatatttt acagatttag agttgctcaa acttggtgat
    19321 aaatttttta ttgatatttc agggaaaaaa cttgcttatc aagtagatcg gataaaaaca
    19381 attctaccaa ccgaatttga ggatttgctt cctgtggcag gaaaagatta tgtaacactt
    19441 atgacatgta cacctgttgg agataattct cataggttac tcgttagagg ggttagcata
    19501 ccttaccaaa aaagtattgt tgagaaagaa aagaagaaag aagatttaaa ggtaaaggcg
    19561 cagatgaact ggtatcctat cgtagttgta ggggttttat taataatagc tgtgatagtc
    19621 attattaaaa aaatagtagc aaagagacga ggaatgaaaa atgaagaata aaatactact
    19681 ttttttgact cttatttgca ctttggtttt aacaatttat cctgcaaatc agctctatca
    19741 tcaattcaaa acaaaagaga atgtggcgga aatccaagag aaaattgaga aaaaacaaac
    19801 tccttcaaaa gaggaagtgg caaaaaaagt cgaacaaata aaatcgaaca gcggtaacca
    19861 aggattagac aatactcaga aaacagataa aggagtagac aatactcagg aaacagacgg
    19921 tggatcattt gatattcgtg aagctgaagg aaaggtcaat ccatacacag gagatgtttt
    19981 ttcaaaaaat caaacgaaag attttgaacc atctacctat tcaggagcgg ttggttatgt
    20041 ctatattcca aaaataaatg tgctagagct tttgtttcta ggcgcttcac aggagcattt
    20101 atatagaggt gtggcacaaa tgagtgctac tagtctacct ttatcaggta aaggtcatca
    20161 aacagtaata gcaggtcatc gtggcggtta tacgtcacca caatttcttt atgttgatca
    20221 attagtagcc ggagaccaca tttatataga ttatcttggt gaaaaagcag tttatacggt
    20281 tttccgaaca gacgttatta gaaattacgg tcagaattca acactgactg atattcctga
    20341 tttagatatg ttaacacttt atacttgtca tccatatcct cagaactacg atcgtttatt
    20401 agttagaagt ctaagagatg aaaaagcaaa atacatcacg ccacaagtaa cagttggcaa
    20461 taccgcatac gctgatgcca ttgatagcga gtccgtttca gataagacag aaactgttac
    20521 atcttctaat gatttatttg aagatgttga tcgtgccaac aaggaattag atggaaataa
    20581 tgatcttaat attccggaac aaggtagcgt agagatgcaa gacagaacgt ttgctgaaaa
    20641 ggcaagttca atatttacag gaattaccaa taaacatcag aaaatgcttt tagtcatttg
    20701 gactgtaata ggagcattag caagtctaaa ggttcttatt cttttaatca aagagtttcg
    20761 aaaagcataa tgaagattat aaaaattgat agagtacagt aaaaagggat tgatactatt
    20821 attgatctct tttttatttt gaatagagct tgattcactt ttaaataaaa tcaaaac
//
LOCUS       DANJMI010000050        20769 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000050 DANJMI010000000
VERSION     DANJMI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.050"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            288..695
                     /locus_tag="PWU81_002213"
     CDS             288..695
                     /locus_tag="PWU81_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110899.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="PWU81_002214"
     CDS             709..1110
                     /locus_tag="PWU81_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110900.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="PWU81_002215"
     CDS             1112..1483
                     /locus_tag="PWU81_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110901.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1519..1818)
                     /locus_tag="PWU81_002216"
     CDS             complement(1519..1818)
                     /locus_tag="PWU81_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110902.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1824..2054)
                     /locus_tag="PWU81_002217"
     CDS             complement(1824..2054)
                     /locus_tag="PWU81_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110903.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(2490..3056)
                     /locus_tag="PWU81_002218"
     CDS             complement(2490..3056)
                     /locus_tag="PWU81_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL1110904.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(3056..4012)
                     /locus_tag="PWU81_002219"
     CDS             complement(3056..4012)
                     /locus_tag="PWU81_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDL1110905.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(4012..4668)
                     /locus_tag="PWU81_002220"
     CDS             complement(4012..4668)
                     /locus_tag="PWU81_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL1110906.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            5038..7056
                     /locus_tag="PWU81_002221"
     CDS             5038..7056
                     /locus_tag="PWU81_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL1110907.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    7126..7332
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7483..9897
                     /locus_tag="PWU81_002222"
     CDS             7483..9897
                     /locus_tag="PWU81_002222"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL1110908.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            10245..11798
                     /locus_tag="PWU81_002223"
     CDS             10245..11798
                     /locus_tag="PWU81_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL1110909.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(12179..13963)
                     /locus_tag="PWU81_002224"
     CDS             complement(12179..13963)
                     /locus_tag="PWU81_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1110910.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(13978..15699)
                     /locus_tag="PWU81_002225"
     CDS             complement(13978..15699)
                     /locus_tag="PWU81_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1110911.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            15799..16560
                     /locus_tag="PWU81_002226"
     CDS             15799..16560
                     /locus_tag="PWU81_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL1110912.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            17527..17795
                     /locus_tag="PWU81_002227"
                     /pseudo
     CDS             17527..17795
                     /locus_tag="PWU81_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            18467..>20769
                     /locus_tag="PWU81_002228"
     CDS             18467..>20769
                     /locus_tag="PWU81_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110913.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
BASE COUNT         6904 a         3598 c         4111 g         6156 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtccggcacg tcagtcacga tatcaaacaa
     1621 aaagtataca cccttcttca ttctagtttt cgcagcagga attttgaaat agttcttatt
     1681 agaatagtag agattgatta ataagctatc ttcgattgct aaaaagaaaa cttctgaatt
     1741 ccatacttta taaaaatctt tgataaatct attcgaaggg tcaaatttaa accataattg
     1801 tgtctcatta aaaagcataa ccattactcc gatctgtttt taaaattagt ttctacctct
     1861 aatatatatc gagtttttat tttgccttca gagaatactg tttctttttt tgcagttaca
     1921 ggttgtttat tttcggaaaa agctaatata gctaagattg aaacatccat ctggaattta
     1981 tcttttttac tgctttgatc ataaaagtct gcatattcat cactgatatt ttttctaata
     2041 aattcttcca tcataaaaat cacctcaaaa cgattatacg aacttacgtt cttgttgtaa
     2101 agcgatattt gaggggcaaa aaaggggcat aatttgactg atattaagtt agctattgat
     2161 atatgaatga catacaaaaa tataaaatct tttaaaaatg cttatatatc aatgtttttg
     2221 ccattctttt atatatctgt tatatgtcaa aaaaatgtgt tcaatttcaa tttgtatttg
     2281 aataaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac
     2341 agcttctcat gtttttgatt tggttggact tattaccgca gatataagct tttgagcacc
     2401 gtgaataaag aggttatgaa atcaacgctc taacctgagt attagagaaa aaaacgcaaa
     2461 acatatcatt tatgttttgc gttttttatt tatctttttt tctcaataca atataaaata
     2521 ggcggctggt tcttttggtt gataaaccga taattcagca cattatatgt ttcctgtggc
     2581 agtttggaac aaaaagaatc aaccatatcc agttcttttt ctccaccttc atgaccatag
     2641 tagacgacaa ggatcaatcg tccgcgaggc acaagtcgtt tcaatatttc ttccatagct
     2701 gtgcgtgtag tctctggcat agtgatgatt gctttatcac tttttgggag ataccctaga
     2761 ttgaaaatcc ctgctttgag attttgttgt tcagctatca cttctcctaa cgtttcgtgg
     2821 ccttgtagaa acaatgagac acgttcttct aactgacgct gttctagcct ttcacgagta
     2881 gcatcgattg cttgttgctg gatatcaaaa ctatagacat gaccagtttt tccgatgtgt
     2941 tctgctagaa aaaccgtatc gtggccattg cccatcgttg catctacgac gtgatcccct
     3001 ggttgcaaga tttcttgcag taatgtatgg ctgaaatgca aagcagtttg caacatcaga
     3061 cgctcacctt tttccgtaca tcatattttc cttggaagct atctctgcga agaagttctt
     3121 catcaattgc attcaagact tcccattttt tcaagctcca cattggtcca atcaaggaat
     3181 cccacggcgc atcccctgtc aaacgatgga tgatgatctc ttttggtatc atttccagct
     3241 ggtcgcaaat gacattgaca tagtcaggac ggctcattaa ttgaaggcga ccttcatgat
     3301 agtcccgcaa cattctcgtt ttgcgcatca gatgcatcaa atggagcttg attccttgga
     3361 tatctgaatc taaaatggtg cgtctgacat tttctttcat catttctaaa ctttcgcctg
     3421 gtaatccgtt gatcaaatgc gtacaaacac ggatattatg tttgcgtaat ttagccacgc
     3481 catctaaata agtttgataa tcatgtgctc ggttgatcag ttttgaagtt tcttcaaaag
     3541 ttgtttgcaa tccaagttct acccataaat acatccgctc gttcagttca gcaaggtaat
     3601 caactacttc atctggcaga caatccggtc gtgtaccgac tgataatcct acgacacctg
     3661 gtaaattgac tacttgctca aatcgttctt tgattatttc aactggcgca tgggtgtttg
     3721 taaaattttg gaaataaacg atgtattgtt ggacatgagg ccatttttga tgcatcatgt
     3781 cgatttcttt ctggaattgg attggaagag ggtcttcagg tgcaacgatc atgtctcccg
     3841 aaccagatac actgcaaaaa gtacaaccgc catgggcaac agtcccatct cgattcggac
     3901 agtcaaatcc tccatcgatc ggcaccttga aaattttccc gccaaactca tttcgtaaag
     3961 cgtaattcca agaatgatat cttttatttc cttctgaata aggaaaagtc attaaaagtc
     4021 cttctttctg taagagttat ctatttgttt tcttttttct ctatacactg gataagtcag
     4081 gcacagccaa gaaagactga gtaagtaacc gccgatgata tcagtaggaa aatgaacgcc
     4141 cagataaatg cggcttatcc cgacacttac tataataaac atcatcagca attgaagcaa
     4201 tgtgcgtaag gttctttttt gaacaaaagc tggaagcaga aatagtaaag aaccgtaaag
     4261 aatcatactg cctgctgcat gtccactagg gaaactgtaa cttgtttcaa ctactaagtg
     4321 cgccaatgaa ggacgctctc gcatgaaaaa tagtttgatc accgggttga tgattccagc
     4381 aattacaata aatccagaaa cgaaccaaaa tgtttctgcc ctttttttct tataccacat
     4441 ccataaagcc gcacaagcaa ataaaatgat gacggtaaga ggattaccga atttagtcat
     4501 aaagagttgg tagctgttca gatcaggata agaagctctg acaatgcctg taaaccactg
     4561 atcgaaacca gaaagccacg acagatggaa ctttacgatc cagcccaaaa tgaaaaacag
     4621 gacaagaaag aagctaccag ccacctgaaa tgatgtttta tatttcatac aattcctcct
     4681 ttgaagcaat gacttattat agcatttttg gtccagcgaa aaaaacggta aagagataaa
     4741 accaatgtat gattcctctt tgaataacca atattcttat aaaaaaaata actgtaatcg
     4801 gaaagacatt ttttggtaat gcaaatgaaa tgttagggaa ataaaatcag tgtttttcga
     4861 aatcctttta aacaagaacg tagacgtagg aagaacttga aaatgaacag aagttgttca
     4921 ttacggtaaa ttacaatatg attacaaaaa acaacaaagc tcttaaataa cttttaataa
     4981 atattaagag aagttataat tattaataat agataaattg aagttggagg atagaagatg
     5041 gaaaacattg caagaaaaga acggagacgt ttagaacaga ccaaacgttt tcgaaaagca
     5101 aaacgagggg cagcaatcgt gggaactgcg atggtaggct gttctgtcgc tgccccatta
     5161 gtacagccgg ttcctgttca agcggatgaa acacctgttc agttcggcgc aagaatcaat
     5221 acagcggcat ttatcacaga aattgctact tatgctcaac caatcgctca agccaacgat
     5281 ctatatgcat cagtgatgat cgcacaagca gttgtagaaa gcggttgggg cagtagtgcg
     5341 ttatctcaag caccttatta taatcttttt gggataaaag gcagctatca aggacaaacc
     5401 gtctacatgg atacgcttga atttttaaat aatcaatggg taaataaaaa ggagcctttc
     5461 cgacaatatc cttcttttgc tgaatctttc aatgacaatg cctatgtact tagaaatact
     5521 tcatttggca acggttatta ctatgccggt acttggaaga gcaacacgaa atcctataca
     5581 gatgcgacag cttgtttgac agggcgttat gcgactgatc ctggttacgc aggcaaatta
     5641 aataatatca tcgcaactta cggattgacc caatatgata caccagctag cggaaacgca
     5701 ggcggtggag cgactacagg tggcaataat actggaagtg caggtagtac tggcggttca
     5761 agcaattctg gtaatacaaa ctcaggttca gcaacttcga ccacttatac tgtccaatct
     5821 ggtgattctg tttggggaat ctcaaataaa ttcggtatca caatggatca attgatccaa
     5881 tggaacaaca tccaaaacaa tttcatttat ccaggacaaa aactgacaat caaaggtagc
     5941 caggaaaatg gctcttcgac gaataattcc ggtaacaata ccaattcttc aggaaatgct
     6001 ggtacatcaa acggaggaca aacaactggt gcgaaataca ctgttcaatc tggtgattct
     6061 gtttggaaga tctcaaatga tcacggaatt acgatggctc aattgattga atggaacaat
     6121 atcaagaaca atttcgttta tccaggacaa cagctaatcg tatcaaatgg cggttcttct
     6181 aatgctggag caaatactgg ctcaaatact gggaatacaa gtggctctag ttcgtccagc
     6241 tcaagttctt ctaatacagc ttcaggaacg aaatatacag ttaaagccgg agactctgtt
     6301 tggagcgtct caaataaata cggaattacg atggctcagc taatccaatg gaacaatatc
     6361 caaaataact ttatctaccc aggacaacaa ctgactgttt caaacagtgg atcaacaaac
     6421 agcacgtcag gtaatacagg ttcgtcaacc aatacaaata acagcagttc agttaatact
     6481 ggcagcagtt cgtctcaagg cacttatacc gtgaaagcag gtgaatctgt ttggagcgtg
     6541 gcaaataaaa acggcatcac gatggatcag ttaatcgaat ggaataatat caaaaacaac
     6601 ttcatttatc caggacagca attgatcgtt aaaggtggaa cttcggcaaa tacaaataca
     6661 ggttcaacta cttctgcagc gaaaccaaat acaccgaata cgtctgctac gacaagtaca
     6721 tcatccactg gtaatacgat gtataccgtg aaagcgggcg agtcagtttg gagcgtagcg
     6781 aacaaacatc atatcacgat ggatcagtta atcgaatgga ataatatcaa aaataatttc
     6841 atctatccag gacagcaagt gattgtgaag aaaggttcat ctcaatcaac aaaccaacaa
     6901 tcagttactg gttcgaaaac ctatactgtg aaatctggag aatctgtttg gagtgttgct
     6961 gacagtcatg gaattacaat ggctcaacta atcgaatgga acaatattaa gaacaacttt
     7021 atttatccag gtcaaacgtt gattgtagct aaataaaata gctgttcgaa gaaataacga
     7081 ttgaattcca caaaaatatt tgttactata aggctactta aatatgacaa cacttgaaaa
     7141 ggaagtagta aaaagactga agtttcagag agcgcatggc tggtgagaat gtgcacggat
     7201 gcttttgaac tcgcctttga gttgcccttg ctgaataccc gagtaagcaa gcggcggtga
     7261 cggccgttac aacgttcgag gccttttcac taaggtaaaa ttaggtggta ccgcgtagat
     7321 tacgtcctat aaaaagagca atctttttat aggatttttt tatttctttt gtgaagtttg
     7381 agaaaacaaa gcaaaaggaa ataaaaataa acagattctg ccggtttttt cgttatattt
     7441 ataatagaaa caagtgtttt caatagaaca ggaggacagc aaatgagtta caatcataaa
     7501 gaaattgaga aaaaatggca aaaatactgg gcaaaaacga atactttcaa cacccatgat
     7561 gatcctgaaa agccaaaatt ttacgcactg gatatgtttc cctatccatc cggtcaaggg
     7621 ctacatgtag ggcatcctga aggttatacg gcaacggata tcctttcacg tgtgaaaaga
     7681 agtcaaggat acaacgtgct gcatccaatg gggtgggatg cattcggtct gcctgctgaa
     7741 caatatgcgc tagatacagg aaacgatcca gctgagttta cccaaaaaaa tatcgaaaca
     7801 ttccgacgcc aaatcaattc tttaggattt agttatgact ggaatcgcga agtgaataca
     7861 actgatccgg agtattataa atggacacaa tggatcttta cgaagcttta tgaaaaaggt
     7921 ctagcttatg aagcagaagt agcggtaaac tgggtacctg aattaggaac agttatttca
     7981 aatgaagaag tgatcgatgg gaaaagtgaa cgtggtggct atgatgtagt cagaaaacca
     8041 atgcgccaat ggatgctgaa aatcactgct tatgctgatc gcttattgga tgatctagaa
     8101 ttagttgatt ggccagaaag cattaaagaa atgcaaagaa attggatcgg tcgttcagta
     8161 ggagcaaata ttgaatttaa agtggctggt acggataaat cctataccgt atttacgaca
     8221 cgaccagata ctctattcgg tgcaacttac tcggttcttg cacctgaact agatcttgtt
     8281 agagaaatta cgacccctga acaaaaagct gctgtagaag cttatatcga agagacagct
     8341 aaaaaatcag atctaaagag aacggatctt gcaaaagaaa aaacaggagt atttactggt
     8401 gcttatgcaa tcaatccagt taatggcaag gaaatcccaa tctggatcgc cgattatgta
     8461 ttatcttcat acggcacagg agccatcatg gctgttccag cacatgatga acgtgacttt
     8521 gaattcgctc aaactttcgg actagaaatt cttccagtta tcgaaggtgg agatgttgaa
     8581 aaagctgctt acacagaaga tggtacgcac atcaactctg aatttctaga cggcatgaac
     8641 aaacaagaag caatcgataa aatgaatgaa tggctagaag aaaatggcgt agggaaaaaa
     8701 gaagtcagct atcgcttacg tgactggcta ttctcccgtc aacgttactg gggtgaacca
     8761 atcccaatca tccattggga ggacggaaca gtcacagctg tcccagaaga agagctacca
     8821 ttacggttgc ctaagacaaa aaacatcaag ccaagcggta ctggagaatc tccattagca
     8881 aatatcgagg agtgggtcaa tgtcgttgat cctgtaacag gtaaaaaagg ccgtcgcgaa
     8941 acgaatacaa tgccacaatg ggcaggaagc tcatggtatt acctgcgtta tatcgaccct
     9001 cataacaaaa aagaactagc agattatgaa aagttgaagc gttggctacc agttgacatc
     9061 tacattggtg gagcagaaca tgcggtactt catttactat atgcacgatt ctggcataaa
     9121 ttcttatacg atatcggtgt tgttcctaca aaagaaccat tccaaaaact atacaatcaa
     9181 ggaatgatct taggcgaaaa caacgaaaaa atgtcaaaat ctcgtggtaa tgtagtcaat
     9241 cctgatgatg tggtcgaaac ttacggtgca gatacactgc gcatgtacga aatgttcatg
     9301 gggccattag atgcttcgat tgcttggagc gaaaatggct tagaaggaag ccgtaaattc
     9361 ttggatcgtg tatggcgttt gatcgttgat gagaacaata aaatgcgtga ccgtatcact
     9421 acattgaatg atggcaagct tgataaagtg tatcatcaaa cagtgaaaaa agtgacagaa
     9481 gactatgaaa atcttcattt caatacagca atctcccaat tgatggtctt tatcaatgaa
     9541 gcctataaag tggacgctct gccttatgaa tatatcgaag gatttgttca actactcgca
     9601 ccgattgctc ctcacatggg tgaagaacta tgggcaatcc ttggaaatga tggtggtatt
     9661 tcttacgcac catggccaac atacgatgaa gcagcacttg ttgaagacga agtagaagtt
     9721 gttttccaag taaacggtaa agttcgtgcg aaaagcaatg ttccccgaga cttaggaaaa
     9781 gatgagctag aaaaagcagc attagccaat gaaatagtgc aagaatatat tgaaggcaaa
     9841 acagtcagaa aagttattgc ggtaccaaac aagttagtca atatcgttgc taattaattg
     9901 gctctttgtc aataaggact gatgagttgt gcaaaatcaa atctgggcca gaatgaactt
     9961 cattctgtct cagatttttt gttatttcaa attgattaat tgattgatca aaaaggtttt
    10021 gattttcatg ttctcaaaca cgttcaatgt ctttttcttc tgaatggtct tgtaagagat
    10081 ctctgtaatc atcaaatttt tgtctattaa tcttagtatt ttttgatgta taaactacct
    10141 gaaattgaga gtacatacca aattcataaa ttttttatta gtgggactag tgattgtatg
    10201 gattttgacg cataatgaat gaagtatgag aaaaggagag acaaatggat caagaactat
    10261 ttattatcga agtattatca gctaaacaaa aaattatcga gaaagcgaaa aaaagagcga
    10321 aaagaataat gttcaagctt ttaagcttag tatttttctc tggtttgctt tttttcggag
    10381 ctgtatcgtt cctatatcgc catatgagtg cagtagtccg ttcagctgaa gggatgcaaa
    10441 aattaaataa tttgtatcac ttttcgtggg gagttggcgg aataacaatt gttgtgctta
    10501 ttgtttttct tctacttatt tggctccgtt accatcaatt gtcgcgtcat ttcgatcaat
    10561 ggcttagtca aagagcacaa cagtttttac ataataaacc aacttctcaa gatgatgact
    10621 atctttattt tgagaagact tcgacgagtc aagcttttcg gatacaaaaa agtgattgcc
    10681 agttattgct gagatcactt gatgatttac ctatctactt aggcgaaagc aaatcaaaat
    10741 tcggaatgaa cacattccat atttttttac tagaaaacga acccagatgg gaaaaaagcc
    10801 agtcttcgcc tggtggatac ctgacaacta agatgagtac attagtcact ggttttttcc
    10861 tcattctttt agctggaaca atctattatg ggagatacac tcagccaaaa atgtcttctc
    10921 atataaatgc tgcttctcac atgacaatga actcaactaa cgatacgcat atacaaaaga
    10981 caaatgaagg gttgttagct gatcctaagc aaaaggtaaa tttgacaaca caaaagataa
    11041 atgatctcaa acttgataca gagacgcact ctctctatat gacaacagat agtggtgcta
    11101 attgggtatt tgtgccgatt gattcgcaat ggttgcgttt tggcgaatac accctgacaa
    11161 cggggctgat tcctgaaggt tattggatgg acaacacata tgatatttcc gccgatttct
    11221 catggtttat ctattcttct ggggatgatg tacgtctgct gacttcaaga gataatggta
    11281 aaacatggca aacgtctatt atcacccaat cagttcagcc gatacgttat cgaaaagcgg
    11341 tattttatgg caacgatggt ctatttatct gtacatcagc cagtgggatg tctgcagaaa
    11401 ctttgtacat ttacactact atggatggcg gaatcacttg gataaaaagt ggaagtacaa
    11461 tgatcaatca gccagtacaa aatgtcagct tcgttacaac ttctttaggt tttgtttcga
    11521 cacgtgaaag tttgttttat acaaacaacg gaggacgctc attcaaagaa tcagttctgt
    11581 cgattccgga aaactatcag acagggggga tagatctttt tcaatcacca aatgaagtta
    11641 ctcaaataag cgcgaatacg ttagagacaa aattttattt gttgaaaaaa ggaccaatcg
    11701 atgcagggaa aatgtttgcc tgcctcttcc gttcaactga taacggggaa acatggcaat
    11761 tcgttcagca actatcgcaa gtagaacaag gagattaaag aagagagatt gtgagcctga
    11821 cgttcagcac cgagtcacaa gaaacaaatt caaaaaacag cttctcatat ttgttgaata
    11881 gtgtaactcg tgattcggag ttgacaagag caacaagaag cgattttgaa gtatagcttc
    11941 tcgtatttta caagatcgtg tcttattgcc gataggttgt ctcttgaaca ccgtttattc
    12001 gtaaaaaggg attgtgacaa gtcttttgtc actacttacc gaacgaaact gaaggagcta
    12061 tccaaaaagt atcattagta ttattctttg tgtttctagt ggcaccacta gtacatttaa
    12121 aaaagctatc gatcatggac taatcataca tgatcgatag ctttttagat aatgtttgtt
    12181 acgctatctg aaattgacta ttataaagac tggcataaag cgttggtgat tggagtagtt
    12241 cagaatgtgt tcctttttcg acgatatcgc cattcttcat tacaaggatc atgtctgcat
    12301 tttcaatcgt tgacaaacgg tgtgcaatca caaaacttgt cctatctttt gtcacagctt
    12361 ccattgcttg ctgaatcaat gcttctgttc gtgtatctac gctagaagtt gcttcatcta
    12421 aaataacgac aggaggattc gctagaatga ctcgagcaat tgtcaataat tgttgctgtc
    12481 cttgagataa cgtgccgttt tcgctagaaa tgatcgtatc atatccgtct ggcagtgttc
    12541 gaataaagtg atcacattgt gcgatttcag ccgcttcgat aatttgttct cttgtggcat
    12601 ccattttccc gtaggcgata ttatcagcta ccgttccttc aaacagccag gtattttgta
    12661 aaaccatccc aaaagaacga cgtagttctc cacgtgaaag cttggtaata tcaagaccgt
    12721 cgaattcgat ctttccttta ttcagttcat aaaaacgcat cagcagattg actaaagtcg
    12781 tttttcctgc accagttgga ccaacgatcg ctaccgtttg ttttggcttt actgaaaaat
    12841 cgacttgatt catcaatatt ttatctgatg tgtaaccaaa ctgtacattt ttgaaagcta
    12901 tttttccttt cggttgttct aaaagtcttg catctggaag gtctggaatt tcatctgttt
    12961 catctaatat ctcaaatatc ctatcgattg ctgccattgc ggattggatc gagttgatca
    13021 cataagaagc ggtagaaatt ggttcagaaa tctgattgat gtactgaaga taagcttgta
    13081 agaagccgat cgtcacacca cctgatagaa caagcatagc accgaatacc gcacttatga
    13141 tgaaggcgag ttgattaatg aaacgaatgg ccggatagat agcacagttc ataaattgag
    13201 cacgtttaaa agcgttgtat tgctttttat ttacttcgtg tatagcagct tctgcatgag
    13261 cttgctgatt gaatgttttg accactagat taccagctaa gatttcttcc attcgattat
    13321 ttaaaacacc cagttcatac tgattctgtt cagaaatctg tttgtttttg ttcgcaatct
    13381 tagttgtaac aattgaactg ccaacgatca gcaataagac gagcaaagta agcttgcttt
    13441 cgatataaaa cagcatcaga ccagcagcag ctattgttac gacagaagta aaaaattgat
    13501 tgatacctgt caataacact tgagaaagtt gattgagacc ggtagttgtc cgactaatga
    13561 tgtcacctac ttgatgattg tcaaaaaacg acatcggtaa tgttttgaat ttttttgtga
    13621 tttcttttcg caccctcaat gtgatctttt cgcttaacgc tgccattgtc cgttcctgaa
    13681 taaatgaagt gatgctgctt aagactgaac agccgatcag caataagaca gggaacaaca
    13741 atgcttgttt gatatcactg aaagaaagct gtccaatacc tgtttttctt atttctgata
    13801 agagatcatc gatcccgatt cccatgatca agggcatggc tacgactaat aggttcccga
    13861 ataaactgca tagaaacaat ccgtaaaaat atttatgttc aggtttgatc attttccaaa
    13921 aacgtatcaa agcacgcgtg ttcaacgttt ttttcttttc catcgtatcc cttctttcta
    13981 tattccttgt gattttgcaa actcctgata agtttggcaa ttagacagta gttcttcatg
    14041 agtaccttgt ccaacgattt ttccttcatc taaaacaatg atgttatctg cttgttggat
    14101 tgtactgagg cgttgagcaa caatcacgag agttttctcc gacatgtgtt tatgaagggc
    14161 tgctcttaat gctgcatctg ttttatagtc taaagcggag aaactatcat cgaatacata
    14221 gatctctgct ggtttgatca acgctcgagc aatacatatc cgctgtttct ggccccctga
    14281 ataattagaa ccgccttgag caacaaagct gtccaatccg tcaggaaggg tgtccactag
    14341 ttcttctaat tgagcgatct tgatcgcttt agacaactct tctgttgagg catattcatt
    14401 tcccatctgc aagttgctta aaattgtccc gctgaataag aaagccttct gagggacata
    14461 actgatgtgg gagcggatct gttcttgagt catttgagta agaggaatac caccaagggt
    14521 tacttttcct atcgtagggt cactaagtct caagagaagt ttcgccaaac tactttttcc
    14581 agatccggtc ccgccaacga tcgctgttgt ttttccttta ggtattgtga aatcaatatc
    14641 ttctagaact ggatcagaga tttcagtata tttaaatgtg acatgttcaa aagcagctgc
    14701 atttgtctga tcaaaggttg gttcagctac tgttttgatc gtctcttcct caatttcgat
    14761 ctctgcttgc agtatttctt ctatccgtcg taatgaggca aacgatttag ggataatgac
    14821 caataccata gctgcgatca tcaagtaagc aagtgtcagc atagagtatt cgactacagc
    14881 agtcacgaca ccgatttgta atgctccttc tgctacaaga tttccgccaa accacaaaat
    14941 agaagcataa acgattccca tgagcaaaaa agcaattgga gtgacccaag caaatattcg
    15001 gttcactttg atcatttgat tagcatagtc agcaaagctc ttattcgttc gcttttcttc
    15061 atatgattga ttatcaaagg cacggatcat attaatccca gtaaagaatt ctctcaatgt
    15121 gatggtgatc cgatccattt ttggctggat actcaaagat aaaggtgccc ctcgtttcat
    15181 cagtcgataa acaatccata cataaaaaac aacagagatc aatgtaaccc atgttagtaa
    15241 tagagaataa cgaaacatca tgagaatggt gaaaacgcaa ataaccggtg ctggtaagat
    15301 catttggcaa aataaaacaa tcatttgctg gacattatcg acatcattcg tcattcttgt
    15361 caaaagagat ccagaactaa aacgactgat atccttcatt gacagctgtt ggaatttacg
    15421 gaaatatttc tcccgcgttt ggaatccaaa cctagcggaa gccaatgctg aaaagtaact
    15481 agaggcaact gcagcgattg ttcctagaaa cgctacaagc agcatttcaa ctccaatttt
    15541 ttttataact gaggcttcgc cacttgctat acctgtgtcg atcagctttg ctactaattt
    15601 tggtacacct aatgtaccgg cgatttctaa acagatcatc acaaaggtaa ggagagcgag
    15661 tcctttgttt ttctgtaaga atgtttttaa gatagacata ttattcctcc ttttttcctg
    15721 atacttttcg attctatacc ttcaagtaac ttgaaggtca atagaaaaaa gaataaactg
    15781 aaaaagaggt gagaaaaaat gaaaaaaatg tatacgattg gccaagtatc ggaactattt
    15841 gatttgccaa aatcaacgat ccgctattgg gatgaacaag gattaatccg ttcaagtaga
    15901 caagaagaga atgattatcg attgtttgat atcgatgata tttttatgct ttatgatatc
    15961 gttttttata gaaaattaga tataccgatc aaacagatga agaatttata tggaaaaaca
    16021 ttgacggagt tatatgagac acttgatgaa acagaaagaa gaatccataa agaactggtg
    16081 gtgatgaaac aaaaacaaaa agagataagg gagcaaaaaa aacagttgaa attgatgatc
    16141 gatacaaacg aggaagaatt tcctgtagaa gagatccctt ttgactgtat gatctcaacg
    16201 gagtttgaag atattgtgga aatcaaaaaa tttctaccta actattcaag ctttgggatg
    16261 atgagtatgc cagcttccag ttcacagacg atgtatggat tttttatcga ccctagtgaa
    16321 gtacatttat ttactcaaga cgtgatttgg gagaaaaaag atacggctgt ttatcgtcga
    16381 ttcttattga aatccgagat gaaccatgcg gaacgtaata atattttaga aatcagagaa
    16441 agaatgtatg aaaaaggatg gaaaacagga gaagtgatcg gccagtatct attgacgaat
    16501 acggatgaaa acaatatcag aactgaatat tatcatgcat ggatagaaat gaaaaaatag
    16561 gaaatatgtg tttaagataa ctcgaaaatg atggttgttt tagttataaa gaaaaatttt
    16621 ctggttcgat tacgggttta atgtggggga ttattagtcg tggatagata gaattttagt
    16681 tttttagagg aaatatgtct ttaaaaatgg aatggttgca attcttagcg atatatacga
    16741 tgtaattgtc ctcttaaaaa agtatggaaa aataattgta ttgggtgtta tgtataattc
    16801 atggtgaact gagactaatg agaagaaaaa ttcatattgt tatgagtgag attgtatcag
    16861 atgagaatga taaattaaat aggtgcataa aagcattaca cttaaaatac attgttgctt
    16921 acattattac aatatgttaa taaggcaaaa ataaatagat ggattgaata aacatatttt
    16981 ttaatcaaat agtccctact gtgactttcc tcagctatcg gtgatactac cgatagcatt
    17041 tttttgctta taaaataatt ataagtattt agagattttt cttctaacat tcttcaaaca
    17101 agaaaaatca caaaggttat tgttattttt atatattctt tttaagaatg attgtttttt
    17161 tatagaaatt ttaaaaaata aaacacgtta ttgatggata gttagtttgt tatttggtaa
    17221 tattatagaa gttgactggt agtgatttat ttttcgtcgt tttataaaaa tgttattttt
    17281 tggaaaaatt cacaaatatg taacgaatgt ttttctggaa tttgctgtgt taaaaagcag
    17341 tatgactaat ataaatacta ttgtcattta tgcaattttt tatggatagt aattttagag
    17401 gggaatcaat gaaggaagaa tagaagggaa aaatagttgc aaatacgcca gttagcaaaa
    17461 atagcaatac atcttcaagc tttaaaattc gagagaatca tcttggtagt ggaatcaact
    17521 acaacaaaag gattaaacca attaatagat agaattacca atggaaaaat agaaaaggta
    17581 gttattctat acaaaagttg tttaattcgc ttcggttatg aacttagtga aaccctttgc
    17641 aatatatggt acagaatata gaaattattg acattacaga aaatgatgaa gatttaatac
    17701 agattgtcac tatttgtaga taccttttac aaggaaaccg agccaataaa gctaagaaga
    17761 tgattaagga gttgatggaa gacgatacaa gttaagaaag tgaaactacg cccaaataaa
    17821 gagcaaaaag agtaattgga cgtggaaaat gcaagaaatg aaccatcgat tcggtggaca
    17881 gtttattagt aatgacggtt tgagaacatc catgacgaga atgaaggaac atcctaaatg
    17941 cgtttagttg aaccaagtgc caaataatat ggccaaataa gcgatcagtt atcgatgaat
    18001 cccaagtatt ctaaactata tattcatttt gatgctaagc attgatatat ttttttggga
    18061 ttgatgaagg ttaaccaata cagggactca ctgatgaaat tctagagtct gaccttggta
    18121 tcaacgattt agtgattgtt tctaatggaa aagtataaaa gtatataagt acattaacaa
    18181 aataactatt agggttacgc tccagcttgt gcaaggcact ggcaggagga atcgtgaaat
    18241 cataattatg gttaagccac acggacaact aaaagtcata caaagctatc gaatcaatca
    18301 acagaagaca atcggcaatc attagctttg tttacagctt acattgcaac gcttgagttg
    18361 aaccgaaaat acgagggagg taacgcagcc attttgagtt ggggaatctt tttagaaaat
    18421 tatcgggtac cttgttgttt agtcaaaatt ataaggggga gtaatagtga ggaacaaatt
    18481 tcataaactg ttaagtatgt ttctagttat gcttattgtg atcaatcaat ttgggaacat
    18541 aacggttcta gctgagtctt tagcagaaca gccagagcgt tcgctgacga tcgaagttga
    18601 tcaagaggag gctgctttgg gtgagcagtt ccagttgcga gtcagaggaa caccagacct
    18661 gctaaatgca ttggtcattg ataagagtga ggatttgatt gaaggggaag tgaagcaact
    18721 caatgattac gaacgtctga ttacttattc ttcccagcat tcaggtaaat acgaattgac
    18781 cgcaagaagc ggtgatattc aatcaaatac ggtctcaata gaaatcagtg taccagaaga
    18841 aagcacaagt gagtctttta atgtaccaga agaaagcaca agtgaatctt ttaatatacc
    18901 agaagaaagc ttaagtgaat cttttaatga agccagatca tctgagccat ctataacgca
    18961 ggaatctcag attgaaagac aagaggagct cttgaattat gacatcggca tctctgctga
    19021 gcgccccact ttgccgagca ggcatacaca aacgttaaag ggatacattc aaggaggcgc
    19081 tggcgatatt cttgaagaat atggggagat ggtcgtacga gttcctagag catcgattgg
    19141 cttaattgag ttcattagag tggaggtcgc tgatgattca cctttaaagc cactggcatt
    19201 ttaccaagaa ggcacagacg ttgtttttcc atttaccttt gattatgaag gattaaatga
    19261 agccgctcag tcgtatacat tcagtatcac gtttgaagtt gctttctttc aatcaggcgg
    19321 tactgagcca aataatgact ttcaagtgag tcttacttat gctgagcgag cagcaaacac
    19381 ggaattttca attacgccta ttccttcatc aagacccatt ttttcgaaat tatactatgg
    19441 ctctagagaa atcgacagcc aaggaaatct tgggaatctg ctgattgacg ggcaagacgt
    19501 gaccaacagt aataaattta ttttgcaggt aaattatgaa ggatcacgaa aagacggtgt
    19561 agtggttacc gatgtactac cgcaaggaac aactttggca ccgaatccga ctacatggag
    19621 ttccactgga aatagagaac ctatttcagg tggggttcgg atcgcagaag taacgaatcc
    19681 aggtgaagca aacgttagaa ctcattatgt ttccgaggaa tttgtggaca aagtatcttt
    19741 taataaggaa acgaatacac ttgttgtgga ctttggtaat ctagcagagg gtcaatcgta
    19801 ttttattgaa tatgctcttg aagtgaccga tgtcgacttg ttcaatgaag gaagacaaga
    19861 ggatgggttt tctcggaatc gagcgagtat gacctacaat aatcaggtaa tgagccgaga
    19921 ctaccgcttg agaatccctg aaagtaatct tccggcaatc tctttaagaa aaagtgtgga
    19981 caaaaatgtt atcaatctca atgaaaatga actaacgtat actctgcatg tctcgatgtt
    20041 ttctggaaca agaaatacgc ttgttattga agatgttttg gaagaaggca tggaatttgt
    20101 gtcatttcta tcagatattc cctctttcat tgatgccgaa gtcaataatg gaatagttcg
    20161 tttcagtgcg aataatacga ttggtggtcc gacaagtttt accctctctt ttaaggtcaa
    20221 tgtagaggcg tatgcggtcg gcgatcaaat tttaaacttt gcaacgcttg tgaatagtga
    20281 caatcgattg aatacgagta ctgtaagaac aaggaaaatc gatgggcgta ttaaagtaat
    20341 taaaactgat gagaatggcc aaaggctggc aggagcaaaa tttgaaattt tatcaagcga
    20401 ttcacaagtg atacaagaag gtataacggc agaaaacgga gaatttttaa gtgaaccgct
    20461 tcctgtaggt agctatcaag tccgagaaac agaagcaccg gaagggtatc ttttagatgc
    20521 gacggtacat gatgttttta taaatgaata ctccatagaa gaagtgacgt taaccattga
    20581 aaacatcctt gatacgggtg gtgttgaact aattaaacat gctgaaggca gtggtgaagt
    20641 ccttcaagga gctgtttttg agttacagaa ccgagaagga gaaacgctac aaaccggttt
    20701 aactacagga gaagatggaa aactggcaat tgacggttta gcaccaggag catatcagct
    20761 ggtagaaac
//
LOCUS       DANJMI010000051        20608 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000051 DANJMI010000000
VERSION     DANJMI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.051"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            112..306
                     /locus_tag="PWU81_002229"
     CDS             112..306
                     /locus_tag="PWU81_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110914.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            575..874
                     /locus_tag="PWU81_002230"
     CDS             575..874
                     /locus_tag="PWU81_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110915.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(971..1405)
                     /locus_tag="PWU81_002231"
     CDS             complement(971..1405)
                     /locus_tag="PWU81_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL1110916.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1637..2347)
                     /locus_tag="PWU81_002232"
     CDS             complement(1637..2347)
                     /locus_tag="PWU81_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL1110917.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2337..3197)
                     /locus_tag="PWU81_002233"
     CDS             complement(2337..3197)
                     /locus_tag="PWU81_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL1110918.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3202..3840)
                     /locus_tag="PWU81_002234"
     CDS             complement(3202..3840)
                     /locus_tag="PWU81_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL1110919.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3855..4154)
                     /locus_tag="PWU81_002235"
     CDS             complement(3855..4154)
                     /locus_tag="PWU81_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1110920.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4197..5681)
                     /locus_tag="PWU81_002236"
     CDS             complement(4197..5681)
                     /locus_tag="PWU81_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL1110921.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5701..6162)
                     /locus_tag="PWU81_002237"
     CDS             complement(5701..6162)
                     /locus_tag="PWU81_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1110922.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="PWU81_002238"
     CDS             complement(6180..7247)
                     /gene="ulaG"
                     /locus_tag="PWU81_002238"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL1110923.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7465..8235
                     /locus_tag="PWU81_002239"
     CDS             7465..8235
                     /locus_tag="PWU81_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL1110924.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8508..9335)
                     /locus_tag="PWU81_002240"
     CDS             complement(8508..9335)
                     /locus_tag="PWU81_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1110925.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9903..11138
                     /locus_tag="PWU81_002241"
     CDS             9903..11138
                     /locus_tag="PWU81_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL1110926.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12137..12397)
                     /locus_tag="PWU81_002242"
     CDS             complement(12137..12397)
                     /locus_tag="PWU81_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL1110927.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12424..13725)
                     /locus_tag="PWU81_002243"
     CDS             complement(12424..13725)
                     /locus_tag="PWU81_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1110928.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14017..14475)
                     /locus_tag="PWU81_002244"
     CDS             complement(14017..14475)
                     /locus_tag="PWU81_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL1110929.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14488..15819)
                     /locus_tag="PWU81_002245"
     CDS             complement(14488..15819)
                     /locus_tag="PWU81_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HDL1110930.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15830..17272)
                     /locus_tag="PWU81_002246"
     CDS             complement(15830..17272)
                     /locus_tag="PWU81_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1110931.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17259..18023)
                     /locus_tag="PWU81_002247"
     CDS             complement(17259..18023)
                     /locus_tag="PWU81_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HDL1110932.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18127..19002)
                     /locus_tag="PWU81_002248"
     CDS             complement(18127..19002)
                     /locus_tag="PWU81_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL1110933.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19257..19341)
                     /locus_tag="PWU81_002249"
     tRNA            complement(19257..19341)
                     /locus_tag="PWU81_002249"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19305..19307),aa:Leu,seq:caa)
     gene            complement(19359..19430)
                     /locus_tag="PWU81_002250"
     tRNA            complement(19359..19430)
                     /locus_tag="PWU81_002250"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19396..19398),aa:Gln,seq:ttg)
     gene            complement(19440..19512)
                     /locus_tag="PWU81_002251"
     tRNA            complement(19440..19512)
                     /locus_tag="PWU81_002251"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19477..19479),aa:His,seq:gtg)
     gene            complement(19574..19658)
                     /locus_tag="PWU81_002252"
     tRNA            complement(19574..19658)
                     /locus_tag="PWU81_002252"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19622..19624),aa:Leu,seq:caa)
     gene            complement(19679..19749)
                     /locus_tag="PWU81_002253"
     tRNA            complement(19679..19749)
                     /locus_tag="PWU81_002253"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19715..19717),aa:Cys,seq:gca)
     gene            complement(19795..19866)
                     /locus_tag="PWU81_002254"
     tRNA            complement(19795..19866)
                     /locus_tag="PWU81_002254"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19832..19834),aa:Gln,seq:ttg)
     gene            complement(19878..19950)
                     /locus_tag="PWU81_002255"
     tRNA            complement(19878..19950)
                     /locus_tag="PWU81_002255"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19915..19917),aa:His,seq:gtg)
     gene            complement(19953..20026)
                     /locus_tag="PWU81_002256"
     tRNA            complement(19953..20026)
                     /locus_tag="PWU81_002256"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19992..19994),aa:Trp,seq:cca)
     gene            complement(20033..20113)
                     /locus_tag="PWU81_002257"
     tRNA            complement(20033..20113)
                     /locus_tag="PWU81_002257"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20077..20079),aa:Tyr,seq:gta)
     gene            complement(20115..20190)
                     /locus_tag="PWU81_002258"
     tRNA            complement(20115..20190)
                     /locus_tag="PWU81_002258"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20155..20157),aa:Phe,seq:gaa)
     gene            complement(20226..20298)
                     /locus_tag="PWU81_002259"
     tRNA            complement(20226..20298)
                     /locus_tag="PWU81_002259"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20263..20265),aa:Asp,seq:gtc)
     gene            complement(20308..20380)
                     /locus_tag="PWU81_002260"
     tRNA            complement(20308..20380)
                     /locus_tag="PWU81_002260"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20345..20347),aa:Val,seq:tac)
     gene            complement(20396..20467)
                     /locus_tag="PWU81_002261"
     tRNA            complement(20396..20467)
                     /locus_tag="PWU81_002261"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20432..20434),aa:Glu,seq:ttc)
     gene            complement(20482..20571)
                     /locus_tag="PWU81_002262"
     tRNA            complement(20482..20571)
                     /locus_tag="PWU81_002262"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20535..20537),aa:Ser,seq:gga)
BASE COUNT         6508 a         4314 c         3269 g         6517 t
ORIGIN      
        1 aaaactttca aattgattct ttttatttat cataaagata tagatacata atcattttca
       61 cttacatata tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat
      121 gtagaagtaa aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa
      181 tatcctttac ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa
      241 ataaagaacg agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa
      301 aagtaaatcc aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa
      361 gattatagaa ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt
      421 ataccatatg attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa
      481 gatgaatcaa atatacttgt cattcatcat gaaatataga gacttataaa attgtttatt
      541 tttataatgt atcagtatag aaaggaaata aaacatggat tatactgttg aatttgacag
      601 tgaactactt aagcaatttc cacaagaaga acatttcatg tataaagaag caatttccca
      661 caaagtgaca gagtatttgg agcataattt ttttcgtata aagccagtgc gtactcgttc
      721 ggcaaaagga atatacgaga tgaaagtcaa aattaataaa aaaaattatc gcatagcatt
      781 tgctttaaaa aaaaatcatg tgttcatttt ttatattagt tcgaatttgc aaaaaattgt
      841 atttgataaa gaggtgagta aaaaaataaa atagctgagc attggaggat agattgagtg
      901 atactaagtc tatcctcttg tttttctaaa gaactaagct agaatttttt cttttgtacg
      961 ttattatatg ttatttgtca ttaccaaaaa gcaccttttt tcttctagta tctatgacat
     1021 aagaaatacc acccatttcc tttacttgaa caaatcctac tgattcataa aatttttttg
     1081 ttttctcagt atcatctgtc aataattcga tttggactgg tccataacta gtgatcttct
     1141 ccaaagcttc attcaaaagc actttgccta ttccttggcg ctgatacctc ggatgaatga
     1201 ggatatcttg aataaatgcg atcgtcgcaa aatcagtgat ccaacgaacg attccgataa
     1261 tcttcccttc cccattacgc tttatcagag attcagattg ttcaaatgct ctagctaacc
     1321 ttgcagtgtc ttttgtgtaa ttcgtccaac caacgctctt atataattca gaaatctcat
     1381 cgatcataag ggaatcaata ttcatttttt tcttcctttc ttatacaatt ctccctctaa
     1441 taatacagtt ctttgactat ttaatctatc acatataact ctcctgtaaa gattctctcg
     1501 attccttaaa attcctcttt ccttttagaa tgcatacttt ttgataaata aaaaggttgt
     1561 gataaatttt cttcacaacc tctgtttctc tctatcaagt gttatctttt aattttctac
     1621 ccttacccta gcatctctat tgcccgtagt atgcatactt tccatgtttg cgaaaataat
     1681 gtttatctag taaatattgc gggatttcac atatattggg gttgatcagc tcgttttctt
     1741 tggccatcag acaaatttca tctaagatca aactgttttc tactgctttc ataggtgaat
     1801 ccccccatgt gaacggtcca tgtccataga caagtactcc agggacttcg tttggatcta
     1861 attttctttc cttaaacgtc tcgacaatca cattccctgt atgaacttcg taagcttctc
     1921 ttacttcttc tttcgttaat tggcgtgtac aaggaacctt accataaaaa gcatcagcat
     1981 gagtcgtgcc ataagctggc aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg
     2041 aatgagtatg agtaatagca gttatatctt caaaagtttg atacaaaaca acatgtgtgg
     2101 ggagatctga agatggtttt aagctatctt cttctattat atttcccgac aaatctgtta
     2161 ccaccatttg atctgcttgc atgcattcat atctgacacc gcttggctta atgacgatga
     2221 ctcctagctc acggtccact tcactcacat tgccccaagt caatttgact aatccgtatc
     2281 gcggcaatgc cagatttgct tcaaggacac gttgcttcat tgctgcaatt ttttcttcac
     2341 aagacatcat agccagcctc cttcaattta gaacttaagt agtcttttgc ctgctggatt
     2401 tctcttcgaa agtcttggct tttttcactc cacatttcaa tcagaaatgt tccatcataa
     2461 tctagacgtt tcaaatgtct taataatcca agaaaatcaa cacaaccgct accaaaagga
     2521 acatcacgaa actggcctga atattctttt gttacagggt aagtatcttt taagtggata
     2581 gcagtgatat aatcgatccc tttctcaagt tctcgagccg gattattttc cggccatgcg
     2641 gacaaattgc ctaggtctgg atatacttgt agaaaaggtg aatgaatctg ttctttgatt
     2701 tctaaaaatt ttgaaatgga gttcataaat ggatcatcca tgatttccac ggaaagaatg
     2761 atgccatatt tagaagcttc ctttacgctt tcttttattc cttcgataaa gtcttctctg
     2821 gaattcattg atttctcttc ataataaaca tcgtatccag ctatttggat tactttgacg
     2881 cttagattat gcgccaaatg gattgctttt ttcattagtt gtttcgctgc ttttttcttt
     2941 tctggatcag cagaaccaaa cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata
     3001 cgtacaccgg agctgaacat ctcttctctc agctcagcta tcttttcttc agaccaatct
     3061 aatcgtgcta accgctcatc cgtctcatca atcgacatct cgataaaatc gaagtccatt
     3121 tcttttacaa gggaaaaacg ttctttccat gaaatatctt tgggtaacgc tttttcataa
     3181 atcccaattt gagccatttc cttaccccca gatacgaatg atctcatctt tgaagttttg
     3241 tgcggccttc atcggatcat ttgctgctgt aataccacga ccagtgataa aggtatacac
     3301 atcgatccct tcaaataagt gtaatgtgtg tggatttaat ccaccagtta cagaaacatt
     3361 aaagcctagt tcaatcagtt ttttgatctt cgataaatct ttttcacccc aagtttcacc
     3421 agataacagc gcatcacggc tttgatggta gattacctgc cggataccga tgttatgcca
     3481 atccatggct tgttcaaaag tccaattgcc gtagagttct acttgcaatt cacctatttc
     3541 tttttgtgct gcctccatag ttggaatcgt tgcacaacaa atgactgtca tccagtctgc
     3601 tcctgctttt gcaacattac tcgccactgt tcctccagcg tctgcacatt tcgtatcagc
     3661 gactagtttt ttatctggga acatactgcg aatacagcga atcgcttttt gtccttcctg
     3721 taagcaaaga attgtgccca cttcaataat atctactaca tcaccaactt tcataacatc
     3781 tcctaatgca tgttctaagt cattgtgatc taacgcaatt tgcaaattcg gcctgctcat
     3841 cttagttcct tcctttactt gtttttttgg taccactttg tttcagcaaa tttttcactg
     3901 atttctttga ctgacatcac atttttgata ccgataactg taactccttt tttctctgcc
     3961 tgagtaaaca tattcaaaaa attgactggc gtaaaaacta catcgtattg gccagctgaa
     4021 ctttttccct ctgaaatggc acaatgatgg attttggtaa ttggtacgtt tagttctttc
     4081 attgcttttt ccacacttct catcatcatc aagcttgttc ctgatccatt tgcacacgat
     4141 actaaaattc tcatcttaat ccctccatta tgagtgttgt ctaatcagat tttcttttat
     4201 actctctgct tttctgcata tgtgtcatag tcttctacaa tcaaaaaata accatcagga
     4261 tttttccgat attgtaactg cggaatagcc aataaaccaa tgacaactac ggcaattcca
     4321 gcataaccta ggactttcat gattactgta aacaatggcc aagctgttgc ccagtcgaac
     4381 atcccgatat aaccaccgta tgttgacatc ccaatccatg tagcgaataa tgctgaaccg
     4441 cctacttgta tgattcctga aagaaacggg aacaggcaag cggctttgaa tccacctcgg
     4501 ttattggcat aaactgctat aacggcatta tcaaagaaca acggaatgaa accggctatg
     4561 acgatcgttg gtgatttcaa taaaatcagt aaaccaatca tcaaaaactg tcccaaagca
     4621 ccaaacaaga acccaatcgt taccgcattt ggcgaaccaa agccaaaagt agcagctacg
     4681 tcaattcctg gaacagctcc tggtaaaatc gtgtttgaga ttccttggaa agattgagtt
     4741 agttcatcga cgaatgtccg aacacctaat tgcaagatcg ccagatatac cgcaaaattt
     4801 aaagaagtcg tcataatgta gaataagaaa ctctgccctt cttgcatgaa ttgcgcttga
     4861 atcaaataat ctttgcctag tacaatcaga atgatcccaa agaaaaatag catgagtaac
     4921 gaagtagcaa ccatattctc attgaaaatc gacaagaatc ctggtaattt gatatcttca
     4981 atttttttgt cttgcttgat tgatctattt ttcttttcat cccgcttttt catccactca
     5041 gctaattttg caaatataaa gattccgaac atttgctgat gtgccactgc aaatccggca
     5101 ccttctgtca attcctgcgt gattcctact gttaagttag aagctactgt ccaataacaa
     5161 cctaaaatca gtcccataaa aatcaatgct tgtatcctac ctagatcagg aaagcaaaac
     5221 agtaaaatcc aaaaagcggt agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg
     5281 gaacgcagtt tcgtatattt ctgaaaacgg actaataaga tatttaaaac aaatgcaatc
     5341 agtaataaaa tcatcacatc tccaaaggta cggccaaatg tctccattaa tccagcatcc
     5401 acagcatttt gtccaaaata aggatctgta accatggcat ccaaattgaa ccgttccttt
     5461 agtccaacca agattggccg aaaattattg accaaaccac cagagccaac agtcaaaata
     5521 aagtaaccga ccgttgcctt taagaatcca gcgatgcatt catgaagcgg gcgtttcagc
     5581 aaaatatagc ctaataacac gataaaacca atcaggtatg ctggctgcgt caagatattt
     5641 gttgcaaaat acgtccaaat gcttaacaaa aattccccca tcttttttct cctctatcaa
     5701 ttaattatat tctgccatca caacttggta atcatcgatg gagttcgtag agatgagacg
     5761 ctcaaccaat ccatctgtca ttaataattc ggataatttt tggatatttt ctagatgttc
     5821 ttctggatct tttgctgcga gtgtaaaaaa caaactagcc gttttctcct cattctcccc
     5881 ttcactttca aaacggattg gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac
     5941 accatcactt ttttctgaag aatgcggcat tgctacttga ggtgcgatca caatataagg
     6001 accgtatttt tctacacaat ggatgatctc ctccacatac gtttcatcaa taatttcctt
     6061 atcgatcaat acaagacagc tttcggcgat agcttcacgc caatccgtga tttctttatc
     6121 tgggtaatga atcaattgat ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct
     6181 tataaaaacg acggaaacgg tgtatcattt tcaatcgaga agacatcttc aaatcctcga
     6241 taaaaattaa actcaagatc atctttgttt gtcggataga tgaatttccc gcctacttgc
     6301 cagatataag gtttaaatcc atacttcagg cgatcctttt tgaatttcca aatttcagtg
     6361 atctcttttg gatcagccat aaaattagac caaatatcgt agtggacagg aatcactact
     6421 tttgcattca atgactccgc catgcgcagc atatcaacag aagtaacttt gtctgttatc
     6481 cctcgtgggt tttcaccata ggcccctaaa cagacatcga ttttgtgttc attaccatgt
     6541 ttggcaaata aattggagta atgtgaatct cctgcatgat aaagattccc tcctgatgtt
     6601 tcgaataaat agtttactgc aatttgatcc atctcctgag gcatttttcc ttttaatacc
     6661 tcttctggat cttcacaagt gaccaacgct gtccgatcaa acgcttctaa agctactaca
     6721 ttgatgtctt tgattttgac tgcatcacct ggctttactg taatcgtttt ttcttcagga
     6781 attccccatt tttgccacgt gcgaaccact tcttttggcc caatgaatgt tgcttcagga
     6841 caattttgat gaactgctgc ggctgtatgg atatctagat gatcggaatg gatatgtgta
     6901 acgattaaag cgtctacatt ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt
     6961 aaattaggct gcatattttg aacgccactc atacgcatca tctgatggcc ttttttcatc
     7021 atgccattcc catgactacg ttttcccgtc ccacaccata gatcacataa aatattcgtt
     7081 ccttcatgac ttttcagcca aattcccgta cagcccagcc accacatagc aaaagttcca
     7141 ggatccacta cttctgtttc gatttcttca ttcaagtatg tgccccactc aggaaatgtt
     7201 tctactaacc acttttcttt tgttacttca ttgacagttt tactcatttg cttgctcccc
     7261 ccatatcgtt gttccaactt acaagtaaat attaatgtgt atcagcatcc atgaaaaccc
     7321 ttttatatga ccgtttgttt tttatatgat tttctttcag taatcaatga ttgtttcagt
     7381 ataataaaga cagatatgaa tgtttaaatg aacaactagt caaataaatc catggatcaa
     7441 gatgtccctc aggaggcaaa agtcatgaaa aactcacttg tcaatattga aaaacgtcaa
     7501 aaagaaattt tggaactgct tcagcagact gaacaactat caactttcga attagctgaa
     7561 tcccttgatg tatctattag tacgatacga agagatttaa atttattaga agaaaaaaac
     7621 gagatcatac gcaattacgg ttattgttct tataattaca aaaaccagac tgattttgat
     7681 caatctggtc ctgaaaggat taaacaagcg attgccagag aagctagtaa atacattaac
     7741 gattatgata cgctgtttat caattctagc tcgactgcgt tgaaaacact ggattttctt
     7801 caatctaacc atttgacagt cattacaaac aacgtgaaaa tcaattactc tccgcacaaa
     7861 gataattgta gttatatttt aactggtgga gagatgcgct ttccaaaaga agcactagtg
     7921 ggcgacatcg cattaaatac gatttcttca gtcaatgcag acatttgtat cattggctgt
     7981 agcggagtcg atgtagtaca tggggtcaca acaaagatat taaatgaatc aacgatcaac
     8041 gaaatgatgg tcaacaagac aatcaaatgt aagattttag tcgctgatca tcgaaaaatc
     8101 ggactgacat caaagttcaa gattgctgat atttcagtct ttgattattt gatcactgat
     8161 aaattttgtt ctgaaaaagt attagaggag atcagaaata cggggataaa aaccattcaa
     8221 gtagattcat tttaagaacc aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat
     8281 aacaaattta tcgataaatg aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga
     8341 tgtatccatc caatcgatac catcataatc tattgcttct tttataaccg ttatatgttg
     8401 cctaaatagg aatcaaacaa tatcgaaaaa aggaggtatg acaaaagtca cacctccttt
     8461 tttcgaataa acggtattca aatcagaaca ctgatttcac aaccacttta tttcaggtcg
     8521 ttttcaatca cctttaccgc gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc
     8581 atatatcttt ctaaataatc atcttctcct aattgcttat ggcaagaagc aatttcataa
     8641 agcaagtatt ccaatgcata agtcgattta tgtttcttat tatactcaat acccttctca
     8701 cataattgga ttgctttatc ataatcctcg attttacggt aataggtggc gatatcataa
     8761 tatacaatgc agaccatcag attatcaaaa tcagtctcac tcaatatttt aagtgtacgc
     8821 tccacataaa ctttcgcaaa atctaattct ccttttgaca agtagatcat tgccacgttt
     8881 gcagctgctt gtaagtgatg gatatctgtt tcggtcgttt gttccaacac ttgattaaaa
     8941 taaaataatg ctgcatctgg ttttttcgca taaagatgat caagcagtcc ttgataaaat
     9001 aaaatttgat ttaaaagatc agaattactt actttttcct gatcgatcgt ttgtaaaatt
     9061 ttataagatt cttggtattt tgatacagaa ttcaatgatt gaatctcatt tatttttttc
     9121 ctgattttgt cttcttctgt ttctactaat atatcggaga tctcgacatt cagctttttg
     9181 caaagcgcac ttaagatatt gatgcctggc gacatattct tgttttcgat attgctgaca
     9241 gttgcttgtt cacaaatgcc ttctgctaat tgtacttgtg ttaacccctt tttctttcgg
     9301 tattctttta gtaatgctcc atctacttta atcaaaacaa tttccttccc tttaaaaaat
     9361 tatttctcct ttatatcata cacaaaaaaa agttctaaac aaccaaaaat gtttagaaca
     9421 gaataaaaat tttaatttta aaataatatt caaattaaac aactggcgta tctgctcctt
     9481 gatttctttt tgtgttatct gaataattat cgtttacagt tataacacat agtgaaaatt
     9541 gtcatggcga gaaagtctaa gaatattttt aaacataact atttctttct tacgtgtgta
     9601 taatgacgac agcacaccta tcaattaata taattatttt atttttttat gttctcatgt
     9661 ttatttttaa tgacacaact tctcgtattt atctggcaca acatataaat cgatcaaaaa
     9721 attgttgcgt gtttttttga aaattggagt ttcaacttga ttacaaaatt taaatttaga
     9781 agttgtgaaa acagcgagag acagtttaat attcaaaata tacataggct actcgaacgc
     9841 tgtgtttttt agtatactag taagaaacga agggatagac tcattccaat aaggaggatt
     9901 tcatgaagac aattcttttt gctcctgcag tattcaattt agctgagaca acacgaatga
     9961 ttgaggttgc caaagctatg aaagaccgtt attactgtgt attctttggc ttttctaaaa
    10021 cttttgctcc tttgatcaca gaaagtggct ttgagtatca tttactagcg ccgatcctta
    10081 cgaaaaaaca agaagaacaa atcataaaat ttgaccagtt gcgtacactt aaaaaccctt
    10141 ttaccaaaga aatggttgcc tctcgtatca ctagtgaaca gagtctgatt gctaagtttg
    10201 agcctcgact gattgtgaca ggttcgaatg tgacaatttt tttatcggcc cgcaataaaa
    10261 agattccttt agtatatgtt aaaccctatg ctttgagtga accttttttt caatcagagc
    10321 atgcgctgat gccccacgag ttacgtttct tcggcccttt aagcaatccc ctttggcact
    10381 ggacaaaaaa gcgtatagta aatacccgat ggatccccaa tgccttcaaa cacgctgcac
    10441 aaaaagaaat gcttccttta ccaaaaacaa gtttacaaat gatggatgct gatctaaatc
    10501 tcatcacaac gcctaaacta tttttggaag aagaacagct gccggaaaat tatcgaatcg
    10561 ttggtccaat ttttgctaag ctcagtactt ccttgcctga agaagtgcta acttttatac
    10621 agcgaaagca acaagaaaga aaacgtatta tctatttcgc aatgggtagt tcaggcaatc
    10681 cgcgtatgtt acgcaaaatt ttgttcttct tacaaaaaca accggatcta gcggttattg
    10741 cacctattga ctatctgctt gatgacattc aatctgactc agaagatctt ttaataacag
    10801 aatatctgtc tagtcatctt ttgcatgaat tcattgattt ttcttttatc cacggcgggg
    10861 aagggaccgt ccaaactgcc tgtgaatccg gcaaaccgtt tatcggtgtg gggatgcaat
    10921 ttgagcaaag ctgtaatatt gactattgtg taaaatacgg aaacgcaatc gccctcacaa
    10981 aaccagtgac tgaaagaaaa ctgcagattg ctctgcgcga agtttcttca cccaagattc
    11041 gtcagcaggc acgcacgctg caagccgttt ttccaataaa tggtccacaa caagcaattc
    11101 aagaaatcga aaccttcctg agcaaccacc caatatagtt tcaagtagaa aaagcttttt
    11161 taatagagac tcatcagtaa tttcgctatt cgagacccag acagtgaaaa tacaaaaaaa
    11221 gtaaatagtg gattgaaaag ttagagttac ttaaattacg gtgtctaaaa aacacaaaat
    11281 catccttaaa ataaaagatc tgacgctatc caaatgagca taggtagcgt ctgccattta
    11341 ttcttagtca aattctttga cacctttctc tggttggatc atctcaacgt ttcttcaaaa
    11401 gaaagaccat aataattagg caaatacatt ttcttaaaat gtgtcatata gatctagaag
    11461 catcgctttg tagagatttg tgccgctagt cccctcaaca attactaaat caaatgtagg
    11521 atttaccact ttatctttga taatcaacct ccctcttctg ttgagacaat gatctcgcaa
    11581 tccgatgcat actcctatat aaactgccag caatccccac atattgagta gatcatgtca
    11641 gaatgttcta tgtcgtcagc aacgatacac taaaagtcac gttcaccatg actgattgct
    11701 attcccagca caattatttc tatacacgtt tcatgatttg aataaacact cacatcatac
    11761 atattaccat tcatacacca tactgcagcc cctactatat ggttgtaagc tcccaaatct
    11821 atatttgact tgatcacttt ggtagtagat ctactaattc taaatccatt gctgataaaa
    11881 tttccatttt ttcctccttc tttctgccaa aacagttaaa acaataataa tctttatgta
    11941 aaaaagggct gtgacaaaag ccttgtcaca gtcccatatt ccgaataaac ggtggtcaaa
    12001 agcttgcatc tgcggtaata agttcaacca aacgaaaaat aagccaacat ttcacaaaaa
    12061 tttaagaagc aattttcgta aatttcttct tatttcttga agctaaccac ttctgttaca
    12121 accactttta gaacatttaa agcttcgatg tgacaaacca tctatgctgc tcgatcataa
    12181 aattgatagc aaaaattcca caaagtacat atgtccccca tgaaaacaaa acattcatat
    12241 gcggtacaat gatctttgga agaatcacga tcaaaaatga tgccagtgcc caaatccatc
    12301 gattgatttg aatatgatat gtagctagca tattcaatat aaggtgacta gccgttaata
    12361 aaaagataaa aacaaaaaat aaaatcagcc aatacattgt ttcccacctt ctaaaaacca
    12421 aacttagaca gtctggactt cgttttctcc acgactttgt tcatctgaca gcatcttttt
    12481 atcataagct tttacaaatg gataatagat aataaatgct atcaatgtgt tgatcatgat
    12541 caagataaca acacgccagt catttcctgc tgaaagataa ccgccgattg gagctggtaa
    12601 tgtccatgtt ggcaaaacag taaatctatt gaccatttct aatttgactg caaaataact
    12661 gataatagtc aaaactaaag gaactaagtt gaacgggatg aacatgtaag gattcaacat
    12721 cataggagct ccaaaaatta tgggctcatt gatattgaag ataccaggaa taatagaaaa
    12781 acgaccaacc tctttcaaga attttgattt acagaagaat ataaacaaaa cagataccac
    12841 gatcgttgct cctgcaccac caattgttac cgtccattga taaaactgtt caggagcgat
    12901 atagggtaat tgggtggcag ctgtaccatc tgccaatgcc tttgcattct catccaacat
    12961 gatcagccac attggacgaa caacagcccc tacaatactc atgccatgga taccaaatga
    13021 ccacaacaaa tggatcaaaa acattggtag taaaacacca agcaaattat tccctaaaat
    13081 attggttaaa ggctcgaata acgataataa ggcagcgttt acatcaaagc ccgcgacgct
    13141 tcgaatcagc caagtaattg ttaaaatgac tgcacctgga atcaatgcct caaaactacg
    13201 ggcaacagca ggtggcactt gttctggcat tttgatagta atatttcgat ttttaaagaa
    13261 acgatatact tccactgcaa aaatcataga taagatcccc gcaaacatcc cagatgctcc
    13321 caaagaactc ataggtaaga cccagccaag aggtgcctct tcaggagtta aaacgtttac
    13381 aggaacttgt aacataaaga acgttcctaa agataatata ccacctgtca cactatctaa
    13441 tccatatgat ttagctagat ccgaacccat aacaaaagtt gcataaatag ccatcaatcc
    13501 catcgtcatc ctaaagggta aaacaatcgt tgccgaataa ggggcaataa attcattcca
    13561 tccaggaaac ggtaaattga taaagaccat aaaaaatgtc ccaatcaaaa tcaatgacaa
    13621 tgttgaaaca accccattac gaattgctaa taaatgacgc tgtgtaccaa tttttgccat
    13681 tggtgggaca agtttctctt cgacccattt catcagcttt tccactaaaa ccactccttt
    13741 tcaataaaat atgaaccatt cattgcatga caacgcaaag aatgttcata actaattata
    13801 taattaagaa attttatttc aatatattct cttaaataat caaatttatt tttaagaatc
    13861 ctgaaaataa gttttctaaa tcataggagc aacaaaaaaa ttcgataatt aacaaaaatc
    13921 aaaatgatta aatctagtga ttgaaacata tcatttcaaa aaaattttgt aaattcaccc
    13981 ataaatatca ccctatttaa cttttttcat cgtacactaa aattcaaaac aactatcttt
    14041 aatctttact tcttggtttt gttttaataa atttccttca aaaatgcctg gagaaactac
    14101 aatgatcacc gtatcttttt gtgcttgctc caacttcttc aaatctataa tagctaacag
    14161 tgtcccttct tttacctcct ggttactttc aacccgtatc tgaaacggac ttccttcgag
    14221 ttcaactgta tctaaaccca tatggatcag tatttttctg ccagattcac tttcgatcgt
    14281 tatggcatgt cgggtaggaa atatattatc gattattcct ttaactggcg catggatata
    14341 tccatcatga ttcgttatag cgtatccaac tcctatcatt tttgaagaaa agacctcgtc
    14401 ttttactgct gttaatggaa taatttgacc actagctaca gcttttagaa tgtttcttgt
    14461 tttaaagaaa tgcacttttt atcacctcta ctttaatgct ggccaatatt ttttgtttac
    14521 tttgatcatt tcatctagta ttttttttgc gactctggca tttggtacgg tttgattcaa
    14581 tgtaaatgct tgtaaagctt tttgataaga accttcaaag taagcatcta ctagtaactt
    14641 ctctactgca acttgtgcct ccatcaatcc tttatgaaaa tcactgattg gttttcttag
    14701 tgtaattggc tccacaccag ttgcaccaac atatgccggt atttcaacaa ctgcatctga
    14761 ccgtagattc ggaatcgccc cttgattagg aacgattagc ataaagcgac gacgatcatc
    14821 attcaacaag gaaatagcta tatcgacgat atattgtcca tgctccccaa aattaaagtt
    14881 caatacttct tccacaatac cgcggcgtat tttatcagcc atttctttgg tatttttctc
    14941 tcgtccatcc atgaccatat tagcacgtgt atactctttg tcagtatgct caacgaccat
    15001 atcaggatat aagtaatact ctaagtaatt attcgggata ttacttggaa aatttttcgt
    15061 gatgactgac atcatttgaa aggttttttg ccaagtttta tctcctatat taaaatcggc
    15121 gacttgcatt tcttttgtta ttagtttttc aataatctct ggcataattt ctcgttgcaa
    15181 ttctttatca taaatcgatg tataccagcc aaagtggttt aaaccataat acgtaacgat
    15241 ccaattctta cgatcataac cgtaattcac tgcaatcgtt tcttcaattg aaatagtcat
    15301 atcacaggca ttaatcattt ttacattcgg atatttacga cgaacagctt cagaaacgat
    15361 cgactctgga tttgtataat tcaaaatcca tgctttgggg gcatagtctt gtatatgatc
    15421 gacaatttcc aataaacctt tgattgagcg catcccataa gcaaatcctc cgagtccaca
    15481 cgtttcttgt cctactaatc catgtttaag tggaatcttt tcgtcctttt ctcgcatctc
    15541 caatcctcca acacgaattt gtgagaaaac aaaatcgcat cctgtaaaag caagctttgg
    15601 atcatccgtt gcacgaatgc gaatatttga aaaacctttt cttttcagca taaatttaac
    15661 aatcaagtac atatccatat ttctttcttc atcaatatca tataaacgta tttcagataa
    15721 tggcaaccgt ttttcattgt tcaaaatggc ttgaacgatt cctggtgtat acgtactccc
    15781 gcctccagct atagtgataa tctgtttttt catcatcatc ttttcctttc tattcttcta
    15841 ataatttatt gaattctgcc ataacataag tcacttctaa gccgatgata atttgataat
    15901 ttttttcagt tggtttaatg atcccgctta tccctaattg cttgattcgc tgttcctcga
    15961 ccatggtggg atccactacc tctacccgta ggcgtgtatt acaactatag acatccagga
    16021 tattggcttt tcctccaaac gcttgtagca tttttgttgc catatattca taatttttac
    16081 ttgctaaccc taactctgtt tcttctaagg ttacttcatt gccaaactct gccttagctt
    16141 cacgtcctaa tgtctgaatg tttctcttat tgatcaaata ataaaaagta acgtaataca
    16201 aggcaaaaaa gaaaattccg attggaagaa tcaaaatagc attggtagcg attcgaaaat
    16261 tgatgagata atcaacaata catgctccga atgtaaaacc aattcgtata ttgaataagt
    16321 aacagacaat ccctgctaaa ccggcaaaaa gtgcatggat cacgtacaac ataggagaaa
    16381 gaaacatgaa actaaattca acaggctcag tgatatttgc aataaatgaa gttcctgcac
    16441 cgcttcccat gattcctttg accatctctt tattttcttt ataagctgct ttatagatag
    16501 ctagacatat agctggtata ccaaacatca ttactacaaa aaaaactgat aaaaacagtc
    16561 cagcctgagg atctccgttg ataaagcgtg tcatctctcc tctgaaaact tccttgctag
    16621 gagatgtata gcttcctaaa tcgtaataaa tatacgtatt caacacatgg tgcagtccaa
    16681 aggggatcag caaacgattc aaaaacataa atatccccac acctaatgct cccatatcaa
    16741 cgagcagttt tgcaaaatta tcaatcccaa tttggatgag cggccagata aagcccaaaa
    16801 aaacagctgc gattgtttgt agaatcatca ccatagtcaa tgggaatttt tcgcccgcaa
    16861 aataagaaaa aactactggt aatttttgtt ctttaaagcg attatacgtc catgccgcta
    16921 ctaaaccgga aataattcca ccaaaaatcc ccatattcac ttctgggtcc ataatttcta
    16981 agcctttttt caaggtagca atcgctaaaa aaccagtaag tgcaggtatt cctttgtcct
    17041 tatttttgac aaaaccgatt gcaactccta tagcaaataa gatatccaag tttccaaaaa
    17101 cggcatttcc agctgcacca attactggga tgttcagcat atcgtcagct gataaacgca
    17161 tgatcaatcc cgctaatggc atcgctgcaa ctggaagtag catcgctctt ccaagcttct
    17221 gcacatttga tttaacattc atttttattc ctcctctatt aaatatcctc atatgttact
    17281 aactcaatca aaggatcatt tttcactttt tgagcaaatg ctgaatgaat taaaaattcg
    17341 acttggtggg tacgaggata cacaaaactt gaaccattta acaattcctg atccaaatac
    17401 gctgcgtgac acatgacttc gatcgtttta tcttctctca atatcatgag taaacttgcc
    17461 aaatattctt ccacactcgt attgatttgt cgtttttttt catcagtttc tcctacgaca
    17521 tcgaaacaag gctcaaaata atcgacatga cgaacttcat cttttcgttc aaagttgctc
    17581 cgaaccggca aatcatattt ttttgctaat gtaacaacta cctcctgatt ttcatccaaa
    17641 gtatgagtat gatgatgact gtctaaatga gttggtatga tccccgcatg ataaattttt
    17701 tgaatctgag cattccattc ctgatacagt tgatctttgt caatgttaaa tggatgctga
    17761 taataagata aggatctgaa ctctccgctt tcgataagag tatcgacagt tttgaggagt
    17821 ggtttgccac acgtcaatgt taaatggact cctacaccta gcctaggcat ttcctttctt
    17881 agtttcacag catgctcgaa acccggcata tttgccatca atgtagttga tgtcaaaatt
    17941 cctcgctgat aagcatccat aatcccataa ttcacaccat ggctatagcc aaaatcatcc
    18001 gaattaaaaa tcacttttcc catttcattc tccttctttt ttctactaaa ataggcgcaa
    18061 ccaaataatc atagtccatt tactatgtgt catttggttg cgcctataga tagttacgcc
    18121 cttattttaa tagattcttg ctagatgaat tgccaaatag atgatttcat tttcttgaat
    18181 ggcaagttga aattgctgtt cgatgatatc tttaatcttt agtgctgtac aatacgcttt
    18241 gctatgttta aatgtttcag taaaaaaacc aatattttct actggcgttt cttcctgata
    18301 aatcaagcgt ttaaccagga atttgatatg aaccattaag cgatgataag tcagtgattc
    18361 tttatcataa acgtttgaat aggatttttc tagatgggac aaaatcgtgg tcgtcatttc
    18421 tactattttt aagctcttta aactattagg ctctcctagc tcagcattaa tgatatgcat
    18481 agtgatgaat cctacttcat ttgaccctaa ctcggtttga tatcgatgat tgattcgctc
    18541 gaccacttct actgctatat catattcatt tggataaagg aatctcattt ctggcaacat
    18601 aattgctgtg acatctctat cttctctttt tcgttgaata gcaaaataaa tatgatctgt
    18661 caatgtaata tagatatttg aatctaatgg agaggagatc ctagctgtta tcagttcaat
    18721 cacttcttcc gcaaaatcca taaattcgat tggaatctgc gagactatct cactgaatcg
    18781 tttgatcttt tctttagaat ctaatacaaa taatttgtca gcatcctcaa catcaaatgg
    18841 cttactcact ttagcagaaa agccaatccc tttccccatc gcaatttttt ctattccatc
    18901 gtcatcgatc aatacagcat tatgattcag ctttttcatc acatacaccg ttaattcacc
    18961 tcctctatat gataaaacaa aacaacttgc agactccttc atacatattt ttgtctccaa
    19021 acaattttta aagacaaaaa acgacctaag aaagaaccct ttcttaggtc gtgcctaatt
    19081 gaatagtagc accctacata atttacaaaa atagtttaac acataataaa agcgttatca
    19141 atatcttttt tactcgtttt atgataatac ttgtatcgaa ttgatttgga ggtaattaca
    19201 taaaaacaaa aaaaatatag caactctcta taagaaagtt gctatattga taattatacc
    19261 ggcggccggg gtcgaaccgg cacgtcctca acggacactg gattttgagt ccagcgcgtc
    19321 tgccaattcc gccacgccgg caaatttaaa aataataact ggggtagctg gattcgaacc
    19381 aacgcatgag ggagtcaaag tcccttgcct taccgcttgg ctatacccca agaataaagg
    19441 gcgaatgatg ggaatcgaac ccacgaatgc cggagccaca atccggtgcg ttaaccactt
    19501 cgccacattc gccatcataa aagatgttat ttaatttctc aataaaaaac taagatgaag
    19561 acatcttagt caataccggc ggccggggtc gaaccggcac gccctcaacg ggcactggat
    19621 tttgagtcca gcgcgtctgc caattccgcc acgccggcaa gttgatttat tagtactaag
    19681 gcggtaaccg gatttgaacc ggtgatgaag gttttgcaga cctctgcctt accacttggc
    19741 tataccgcca ttatactatt atcaatctat aaaataaaga ataaattata gaaactgggg
    19801 tagctggatt cgaaccaacg catgagggag tcaaagtccc ttgccttacc gcttggctat
    19861 accccaagaa taaaaagggc gaatgatggg aatcgaaccc acgaatgccg gagccacaat
    19921 ccggtgcgtt aaccacttcg ccacattcgc catggcaggg gcagcaggaa ttgaacccac
    19981 actaacggtt ttggagaccg ctgttctacc tttaaactat gcccctataa aatggaggag
    20041 agtggattcg aaccactgaa ccctaaggaa cggatttaca gtccgtcgcg tttagccact
    20101 tcgctactcc tccatggtgg cgcgggacag aatcgaactg ccgacacacg gagcttcaat
    20161 ccgttgctct accaactgag ctaccgcgcc atattttaaa attattaatt gaataacaag
    20221 aacgacggtt ccgacgggaa tcgaacccgc gatctcctgc gtgacaggca ggcatgttaa
    20281 cccctacacc acggaaccag ttcattatgg aggttaacgg gatcgaaccg ctgaccctct
    20341 gcttgtaagg cagatgctct cccagctgag ctaaacctcc aacattcaat taaaatgacc
    20401 cgtacgggat tcgaacccgt gttaccgccg tgaaagggcg gtgtcttaac cgcttgacca
    20461 acgggccagg taaatttgct acggagagta agggattcga acccttgaga cagtttggca
    20521 ccatctacat gatttccaat catgctcctt cggcctctcg gacaactctc cagagtgaaa
    20581 gaagctcaat actctttctc aaaatgag
//
LOCUS       DANJMI010000052        19629 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000052 DANJMI010000000
VERSION     DANJMI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.052"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="PWU81_002263"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="PWU81_002263"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL1110934.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="PWU81_002264"
     CDS             complement(1172..1867)
                     /locus_tag="PWU81_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDL1110935.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="PWU81_002265"
     CDS             complement(1880..2596)
                     /locus_tag="PWU81_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL1110936.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="PWU81_002266"
     CDS             2926..3534
                     /locus_tag="PWU81_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDL1110937.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="PWU81_002267"
     CDS             3859..4182
                     /locus_tag="PWU81_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL1110938.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="PWU81_002268"
     CDS             4364..5734
                     /locus_tag="PWU81_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL1110939.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="PWU81_002269"
     CDS             complement(5864..7165)
                     /locus_tag="PWU81_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL1110940.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="PWU81_002270"
     CDS             complement(7519..9477)
                     /locus_tag="PWU81_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HDL1110941.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="PWU81_002271"
     CDS             complement(9654..9920)
                     /locus_tag="PWU81_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL1110942.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="PWU81_002272"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="PWU81_002272"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL1110943.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="PWU81_002273"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="PWU81_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL1110944.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="PWU81_002274"
     CDS             13264..13827
                     /locus_tag="PWU81_002274"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL1110945.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="PWU81_002275"
     CDS             complement(14080..14601)
                     /locus_tag="PWU81_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDL1110946.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="PWU81_002276"
     CDS             complement(14604..15446)
                     /locus_tag="PWU81_002276"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL1110947.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="PWU81_002277"
     CDS             complement(15439..16164)
                     /locus_tag="PWU81_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDL1110948.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="PWU81_002278"
     CDS             16340..16636
                     /locus_tag="PWU81_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110949.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="PWU81_002279"
     CDS             17204..18796
                     /locus_tag="PWU81_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL1110950.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="PWU81_002280"
     CDS             18809..19549
                     /locus_tag="PWU81_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL1110951.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5617 a         4028 c         3724 g         6260 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaagtaaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgagatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttc
//
LOCUS       DANJMI010000053        18676 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000053 DANJMI010000000
VERSION     DANJMI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.053"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(541..1566)
                     /locus_tag="PWU81_002281"
     CDS             complement(541..1566)
                     /locus_tag="PWU81_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1110952.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1841..3526
                     /locus_tag="PWU81_002282"
     CDS             1841..3526
                     /locus_tag="PWU81_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HDL1110953.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3540..4823
                     /locus_tag="PWU81_002283"
     CDS             3540..4823
                     /locus_tag="PWU81_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL1110954.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4893..5762
                     /locus_tag="PWU81_002284"
     CDS             4893..5762
                     /locus_tag="PWU81_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1110955.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5759..6595
                     /locus_tag="PWU81_002285"
     CDS             5759..6595
                     /locus_tag="PWU81_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1110956.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6621..7688
                     /locus_tag="PWU81_002286"
     CDS             6621..7688
                     /locus_tag="PWU81_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDL1110957.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7685..8512
                     /locus_tag="PWU81_002287"
     CDS             7685..8512
                     /locus_tag="PWU81_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL1110958.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8557..9582
                     /locus_tag="PWU81_002288"
     CDS             8557..9582
                     /locus_tag="PWU81_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL1110959.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9579..11762
                     /locus_tag="PWU81_002289"
     CDS             9579..11762
                     /locus_tag="PWU81_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL1110960.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11762..12892
                     /locus_tag="PWU81_002290"
     CDS             11762..12892
                     /locus_tag="PWU81_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL1110961.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12889..13566
                     /gene="pgmB"
                     /locus_tag="PWU81_002291"
     CDS             12889..13566
                     /gene="pgmB"
                     /locus_tag="PWU81_002291"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL1110962.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13650..14177
                     /gene="msrA"
                     /locus_tag="PWU81_002292"
     CDS             13650..14177
                     /gene="msrA"
                     /locus_tag="PWU81_002292"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL1110963.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14378..15064)
                     /locus_tag="PWU81_002293"
     CDS             complement(14378..15064)
                     /locus_tag="PWU81_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL1110964.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15144..16682
                     /locus_tag="PWU81_002294"
     CDS             15144..16682
                     /locus_tag="PWU81_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL1110965.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16802..17269
                     /locus_tag="PWU81_002295"
     CDS             16802..17269
                     /locus_tag="PWU81_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL1110966.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17788..18231
                     /gene="rplM"
                     /locus_tag="PWU81_002296"
     CDS             17788..18231
                     /gene="rplM"
                     /locus_tag="PWU81_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL1110967.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18247..18639
                     /gene="rpsI"
                     /locus_tag="PWU81_002297"
     CDS             18247..18639
                     /gene="rpsI"
                     /locus_tag="PWU81_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL1110968.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
BASE COUNT         6071 a         3398 c         4016 g         5191 t
ORIGIN      
        1 gtctaaaata atttgtctaa ctagaagaaa agtgggaaag gatcttaaca acatcggttc
       61 gtatccaatt aaggtatcgg aacgatcatc ttccaagaga agtcaatcaa caaaagtaaa
      121 gatagatcat tttttgaatg caaaataaat tgatgggaag atagagcggt tatttttaat
      181 attttgctag ctattattga ataattcctc gaataaaaca aaaccagtag acatgtctgt
      241 tattctattt atgttttaga gataagtatc cccaaataac atggattgaa ataagggtaa
      301 atgtgtttaa aaaaacgaac tataaaaact tatacacaac gtggttaaaa atagtttttt
      361 cttccctgtg gaaaaaatag tttaacacaa acattatagt tatccacagc tatctaaaac
      421 agatggaaaa taagaaaaat tcgctaaaat accgggaggg ggtaacgagg gatgagtgat
      481 tgaaaaaaga acagagtaac tgacttatcc tcagtgtttc tgttctttta taattctgtt
      541 ctacatttct tgttgttgga tcgaatctgt gagagtaatc tcggttgaaa gaatgattct
      601 tttttcttca gggtattggt ggtggattag ttggaataaa agttctgtgg ctttgattcc
      661 taactgattt gtcggaatgt ctatccgagt caactgtggc gaaaataggg aggacaataa
      721 gggatcactg aatcctgcga tcgaaagatc atcaggtatc tgctttccag attgttctgc
      781 cactttgtaa atactcaatg attttaatgc atcggtagaa aaaatcgcag ttggtctttc
      841 tcgtagcttc attaaatcat tcattgctgc catgctatct tctaagcgaa agccgccgtc
      901 tataatccat tcattacgga tatctaggtg gtgatcaaat aagcattgac ggtaaccatt
      961 gagacgatca ttggatacat aaacttctgt cggagcatgg agacagccga tatgacggtg
     1021 tccattcttg atcagtgttt ccaccaaatc gtaactatcc tggaaattat ccgtgtccac
     1081 tgagtagatg ttcgtgtagt cgtttccgac ttttccgatt accacaatag gaatattgta
     1141 ttgatccaat tcaaaaagaa gtgtttcatc catcgtagag cttaaaataa taattcctcg
     1201 gatcattttt tcttggatct ttttcgcgca tttttctagc tcttcttctg gtgtttttgc
     1261 cgtttgtaga atcaagtcga agttattttg ttcagaaacg gcagaaatgg attgaataac
     1321 atctgaaaaa aaaggattgc cataggtcgt atgagaagta cgatgagata atgctagaat
     1381 cgcgtcaaaa ccatttcctg acagtcctct tgcaattttg ctcggttcat aattcaattc
     1441 tttgattgct tgaagtacac ttgcttttgc tttatcagag atatttggct ggttatttaa
     1501 tactctggag acagttgatt ttgagacatt agcttttttc gcaatgtcat aaatagtagg
     1561 tttcaatttt atatgcacct cctttttggg agcgttttag aataattcca tagtacaata
     1621 gacattcttt tttgtctatg attttgaaaa tttaggaata gcttgcatga tcatgagaga
     1681 ccatattttt ttaagaagct attaattatt gatacataag ctttcttatt tgtatttttg
     1741 tgtatatcaa aattatttaa aaaaatgcta ttgacagtaa gcgttttcca taatatgatc
     1801 aaatcgttgg gaacgctccc aaaaaagaaa ggggaatccg atgtatacaa aacttttaca
     1861 agcgatttat cgcgactcag atttgtctga gaaaaccgcc tctatgattg aacaaaaagt
     1921 cagtgtttat cagcagaaga aaggccaggc aggcaaactg cattgggacg aagaagatat
     1981 tttcttgatt acttatgggg atcaattttt tgaagagcag caaccaactt tgcgtacatt
     2041 taagaaattt tatcaaaagt atttggcaga tacatttaga gttgtacatt tcttgccatt
     2101 tttcccatat acatctgatg atggtttctc cgttgtaaat tatcaggcaa tcaacgaccg
     2161 tttgggcaca tgggaagata tagaagaaat gagaagcgaa acacggttaa tgttcgactt
     2221 tgtctgcaat catatgtctg ccgaaagtac ttggtttcag gaatatttaa acttgaatga
     2281 tacgtacaaa gattttttta ttgatttgcc actggataca gatacaagca gcgtgactag
     2341 accaaggacc agcccgttac ttacaacgtt cattgacaaa aacgggaaag aaaaacacgt
     2401 atggacgact tttagcgaag atcaagtaga cttgaatttt aaaaatccag aagttttatt
     2461 gaaaatgatc gatattcttt tgttttactt agaaaaaggc gcttcattta ttcgcttgga
     2521 cgctgtggga tttttatgga aagaactagg aacttcttgt atacacctgc cagaaacgca
     2581 taaaatcatc caattattcc gtgcaatcgc tgatgaagca gctccaggaa cggtattgat
     2641 cactgagaca aatgtgccac acaaagataa tatcagttat tttgggaatg gcgagaatga
     2701 agcacagatg gtctatcagt tttcgctacc acctttgacc cttcaagcga ttcgttcggg
     2761 taatacaagt aagttgaaag aatggttaaa aaccatcgat ttcgcatcga aaaaaacaac
     2821 tttttttaat tttttagcct cccatgacgg tatcggattg aatccggtac gaggtattct
     2881 gccggaaaaa acgatccttg aattagtgga acagctgaag aaagagggta cccgagtaaa
     2941 ctacaagaaa aacccagatg gaacgaccag tccatatgag ataaacgcta cgtatctaga
     3001 tgcgttaagt tctccaacag atacgacgga atggagaagc aaacgctttt tagctgctca
     3061 ttcatttttg gtaacgttac caggcgtgcc ggcaatttat attcaaagtg tgctgggaag
     3121 tcgaaatgat tacaaaggcg tagaaaaaac aggagagaat cgaaaaatca atcggcaaaa
     3181 atttttgtta gcagatattg agaaagagtt gcagacagat ccattaagaa cagcagtata
     3241 tggtgaattg acccagatgc tatctattcg aaaaaaagaa aaagccttcc atccgaatag
     3301 tttgatttac ttactggact cagcagattc ttttatcagt tttattcgcg aagcgaagga
     3361 agagaaagtc ttagttatcc ataatttttc caatcaatca gcaggttatc ttttaccaaa
     3421 aggagattac ctagacctgt tttctggaca aattatccat tgcagtcaag aacgaaagtt
     3481 gatattccag ccgtatgaat atatgtggct gaaaataata aaataagagg tggaaaaaaa
     3541 tgaaaaaaat tattgttact acctgtctgc tagctactgg atttcttctt actgcttgtg
     3601 gtgaaagtca aagctctaac gaatcacaac agacaacgat tcaattcatg cactcttctg
     3661 tcgaacagga acgattaagt gtgatcaatg aactggttgc tgattttgaa aaagaaaatc
     3721 cagacatcaa aatcaagcaa gtgccggtag aagaatcagc gttcaataca aaaatcgtta
     3781 cgttagctcg ttccggtaaa ttaccagaag taatggaagt cagtcaggat tttgctaaag
     3841 tgatggataa agatgaactg attgatcaga cggcagtcca aaacgttatc gaggaagctg
     3901 gagaagataa ttattatgac ggagccaaaa accttgttcg ttcagaagat ggaaaaagct
     3961 atattgctgc accaattagc ggctgggttc aaggtatttg gtatgacaaa gaaaaattat
     4021 cagaagctgg tttttcagaa ccagaaaatt gggatgatat tttaaaaatt gctcaacatt
     4081 tcacagataa agagaataaa caatatggaa ttgctatgcc tactgctgaa ggaacaatga
     4141 gtgagcaagc gttctctcaa tttgctttgt caaatggagc aaatgttttg gatgatgaag
     4201 gcgaggtaac gatcgatacg gaaaagatga gagaagcatt gtcgttttat caaaacttat
     4261 cccaatacac gatgccaggc tctaatgatg tgacagaaat caaagatgct ttcatgaatg
     4321 gaacagcacc aatggcaatc tattcaactt acattttgcc ttctgtttat gaagcgggga
     4381 actcagaaaa tatcggtttt gctatcccaa cacaaaaaga tcaagctgtc tatggcacag
     4441 tatctggatt gacgatttct tctggtctag acgagaaaca aaaagaggct gctgaagcat
     4501 tcacagcttt cttatctcaa ccgaaaaaca tggaaaagtg ggtcttgatg tctcctggtg
     4561 gtgctcaacc agtgaataaa caagtagtcg aactggatgg ttacaaagaa aatgaagtca
     4621 tcaagtcttt tggcgagctg ccatctgaga ttgctagtgc ttttgatgaa gtacaagtct
     4681 ttggactagt cggggaaaag aatttcacga aaatgggaga tatcacaagc tcaggtgtga
     4741 tcggaaaagc agtgaaccaa gtaactgtcg gaaacgaaga tgtcgatcaa gctttagctg
     4801 acgcacaaaa aaatacgaaa taacaagaag ttctgccaat ggtgttcgaa aaaagtagat
     4861 agctattggc agattatcag ggggaggttt caatgaatcg tccaaggaaa cattctgact
     4921 taaaattagc catgggactt ttagcaccaa gcatgctttt attagctgtt ttagtgctga
     4981 ttccaatgct ttccaatatt cagatcagtt ttttcgatat gccgctgaat cctaactcag
     5041 acgccgtttt tatcggatta agcaattatc ttgaaatttt aagtgatagt gaattttatc
     5101 gttctttagg tttaacagtt gcttacacat tcttagttgt agcaggcagt acagctttag
     5161 gattagccgt agctattttg cttaaccgca agttcagatt taggaaaacg gtgcgttcac
     5221 tgattttatt aagttatgtt acgccatcta tttctttgat ttttgcttgg aaatatatgt
     5281 tcaataatag ttatggaatc gtcaatgatc tgttagtaga taggttgcag cttttcaacg
     5341 aagcaccgct gtggttcgac cagccagttg ctagcttctt tatggtcgtt ttgtttgcta
     5401 tctggagata ctatccgtat gcgtttattt catttttggc tattttgcaa acagtcgaca
     5461 aaacgcttta tgaagcagct gaaatggacg gtgcaaatgc ttggcagaag ttcaagatcg
     5521 tgacactgcc ggcaatcaaa ccagtactag caactgtgat tgttttacga tctatctgga
     5581 tgttctatat gtttacagat gtctatctac tgacgaataa agtcaatatc ttaggcgttt
     5641 atttgtatca gacagccttt gcgttcaatg atcttggaaa agcagcagct atatcgattc
     5701 tattgttcat cattattgca gcggtcgtct tgctctcacg aaagaaggtg gacttgaaat
     5761 gaaaaataaa aagagcagga aaatcttatt ttacgcgacg attttgttgc tattagctgt
     5821 caatttgttt ccatttttga tcatgctgat gacatcattc aaggattcac aagaagcaat
     5881 tgcaacgaca caaactattt ttccgaaaga atggactttt gaacattatc aggatatttt
     5941 caatgctgag atttttccat tcatcatgta tttcaaaaat agtttagtga tctccttgat
     6001 agcctccgta actgcagtag tgattgggat cagcggggca tacgctttgt caaaactacg
     6061 cttcaaagct cgctcgaaaa tcaatgctag cttttacacc gtttatatgt tttccggtat
     6121 tttgatcgtt gttccattat ttaaaatcat ttccggtcta ggtttgtatg ataccaatac
     6181 agcactgatt atcacgatgg tcgttcaaac acttcctact gcgatattca tgctaaaaag
     6241 ttattttgac acgattccga ctgatattga agaagcagca atgatcgatg gtcttagccg
     6301 gatccagatc attattcgga tcattatacc attggctatt tctggaatca tttctgtctt
     6361 cgtttattgc tttatggttt cgtggaatga ttatttattt gcttcgattt tcctgtcgac
     6421 ctccgaaaaa tttactttac caatcggttt gaacacatta ttcagtacac cagactacat
     6481 ttggggacga atgatggccg cttcattggt gaccgcttta ccagtcatca ttatgtatgc
     6541 gttatctgaa cgatttatta aaggtgattt agtcgaaggc ggcgtaaaag gataaaaaga
     6601 aaagaaagag gaaagaaaag atgaaaaaat tagttgcatt agaaccaaga atagccggat
     6661 tcaaagatta cgaagaccgt gagatcgaaa agaacgaagt acgtatccga gtaattttcg
     6721 ctactccaaa accaggaaca gaaattgtcg actttagagg attaagtcca tttatcgatg
     6781 aagaattttc agaagaatgg cgcctattct tgccaagaga agaaggaagc caacgcggtg
     6841 tggtatttgg cgaatttcaa ttaggaaata tgattgtcgg tacgatcgaa gaaacgggca
     6901 gtttggtgac agagtatcaa aaaggagagt tagtctgcac ttatggaccc attcgtgaaa
     6961 cgctgattac aaacggtgtt gataattata aactacgtaa acttgatcgg ttggatcaat
     7021 ggaaaaatgc agtatgttac gatcctgctc aattcgcttt aggtggtatc cgggaagcac
     7081 atgttcgagc aggtgatgct gtagcaatct tcggtttagg cgctatcggg caaattgcga
     7141 ttcaattagc taaaaaagca ggtgctacta ccatcgttgg gatcgatccc atagcgcagc
     7201 gtagagaatt agcagaaaaa tacggtgccg attacacgct agatcctatg aaagcagatg
     7261 tcggttttcg tctaaaggaa ttgacagata aacgtggagt agattcagtt attgaaacga
     7321 gtggcagtgc tgccgcatta caagcttctt taagagggat cggctatggc gggatcattt
     7381 cttatgttgc ctttgctaaa ccttttccag ctgggtttaa tcttggtcgc gaagcacatt
     7441 tcaataatgc aaaaatcgtc tttgctcgag catctagtga accaaaccca gattacccaa
     7501 gatggaaccg taaacgaatc gaggacacat gttgggaatt attgatgaac ggttacttgg
     7561 attgtgaaga aatcattgat ccagttgtgc catttgcaga aagcgcagaa gcttacatgc
     7621 gttatgtaga ccagcaccca gaagaaagta tcaaaatggg cgttcaagtc cgaggaggag
     7681 aacaatgaaa ttcggtacgc aagatcaagc atttttccca agagacattt tagaaaaatt
     7741 tagatttatc aaagactgtg gatttgaaga atacgaaatc gacggtaagt tgttaataga
     7801 aaatatcgac acagtcaaac aggcaatcaa agaaacaggg atcccagtag tgacagcttg
     7861 taacggctac gatgggtgga tcggtgactt tatcgaagaa cggcgtctta atggtgtacg
     7921 tcaaattgct gagattttgc gtgcactaag cgaggttgga ggcaaaggaa tcgttatccc
     7981 agcagcttgg ggaatgttca cttatcgatt accgccgatg gtttcacccc gaagcaaaga
     8041 aggagactat aaagcagtca gcgaatcact tcgttattta gataaagtag ccgaagaaac
     8101 aggtacggtt atttatttgg agcctttgaa ccgataccaa gaccatatga tcaatttgtt
     8161 agcggatgcg cgtcattaca ttgactctaa tcaattgaaa catgtgcaga tcatcgctga
     8221 tttttatcat atgaatattg aagaagatga tatttcaaaa gcattgacta cgcaaaaaga
     8281 gtatgtgggt catatccatt tagcagataa tcatcgctac caaccaggat ctggcgcaat
     8341 tgattttact cgccattttc aaacgctact tcaaaatgag tatgaaggtt atatggtata
     8401 tgaatgtcgt ttgcgaggag aaaatgaagt gaaaatttat caggaatcat tgcaatactt
     8461 gaaaaactgt ctggaaacag cgcgtcattt aaatgattta gagaaagaat agagaaagaa
     8521 taacaataag taaataagca aaggagggag aaataaatga agaaagtagc aatcataggc
     8581 gctggacaag tagcagaaaa agtacatgct gcttattatc aaaaaagatc agatgtctta
     8641 gaattggccg ctgtggttga tccagtcagc cagcgagcag aagacttttg ccgacgtaca
     8701 ggattttctc atgcgtatac atcagttaca caaatgctga atgagatttc tcccgatatc
     8761 gtgagtgtct gtacacccaa tcgctttcac ttcgaacatg tattggctgc cctcgaagct
     8821 aatgcttcag tgatgtgcga gaaaccacca gctatgacag ctgatcaagc aagaaagatg
     8881 gcagagacag ccgataaaaa cgggcagatt ttggcttatg gattccagca tcgtttttct
     8941 gacgaagcaa caatgatcaa gaatcatctt gatgttctcg gggatattta ttacgtagaa
     9001 gcgaatgcgc ttcgtcggtc tggtgttcca ggctggggaa cgtttatcga caaagaactt
     9061 caaggaggcg ggcctttgat cgattatggc attcacatgc ttgatacagc gatgtatatt
     9121 actggatttc ctaaagtaaa aagcgttcgg gcgtacagct tccaaaaaat tggtacgaaa
     9181 aaaaattcag ggaaattcgg gaaatgggat ccaagcaaat tcacagtaga agatgcaatt
     9241 tttggcacac tggaattaga aaacggcaag atcatccaac tgaatacttc gtttgctttg
     9301 aatatcagag aagacaaact gaatgtggct ttttgtggcg atcaggcagg tgctaatctg
     9361 tatccaggcg aactattcga agatgtagat ggaaagctgg tttccttaca aaagaccacg
     9421 ccagttaatg acccacatga actaagtatg gctcattttg tgaatcgggc gctcggtgac
     9481 cagactcaga tgattgcaga tggctggcaa ggatatgtca tccagaatct ggtggaaaat
     9541 ttatatcgtt cggcagaaac aggagaagtt attaccttat gaaagtagaa gaaaaacagt
     9601 ttgatttaga gtatatcaat aagtatgcga gtcagatgac cattggaaat ggattttttg
     9661 gtatcagagg aagccaggag gaagactatc ggaatcaagt aaggggaatg tttgcagctg
     9721 gtgtatataa ccgaccttta ggagcagaat cagccgagtt agtgaatctc ccagatgtca
     9781 tcagatggga aataaaaatc gacggtgaac atttttctct tgagacgatg aaagttcttg
     9841 catacgagcg ggcactagat ttagaaatgg gagagcttaa acgctatgcg ctcattgaaa
     9901 ctgctcaagg aaaaaaagtg gaagtcatta cttgtcgtgt tgctgatcaa aataatctcc
     9961 atcaggcagc cctgaaagtg gtcatcaagc cgttgaccga agactgccaa gtagatgtga
    10021 aaacgggtat cgatggacag caaaccaatt ttggtacaca gcagttggtt gaaaaagaac
    10081 tgcgagtatt tggtgacgat ttgatcagtg catcctacca gacaacagaa agtaaaattg
    10141 atattgggat tgcagctaaa ttcaataaag caggtgtatt ccaagcaaaa aatcgccgga
    10201 tcaccgcttc cttttcagaa aagatcagaa aaaatacgtc ctttacgctg gagaagatca
    10261 tcgtgctctc tacctctctt cagcaaacag acccgtttaa gaaaagtatt gcccaagccc
    10321 agaaaagcgt tagttataca gaaattttag agcagtcgat gtcttattgg acagattttt
    10381 ggcatcagca tcggataacg atccaaggcg attcttcttt cgatcagtta gcgatggact
    10441 tcgctgctta ccatttacag attatgactc cccgcaatga tccaagtgtt agtgtagggg
    10501 cgaagggact aactggcgaa ggttataaag ggcatgtttt ctgggatacg gaaattttta
    10561 tcctgccttt ctttttatat cagtttcctg aagtagcaaa gaatttgctg ctgtaccgtt
    10621 atgatcgatt agaaggcgca cggcaaaaag caagagatta tcactatcaa ggtgcacttt
    10681 tcccatggga aagcgcacac actggtcagg aagagacacc caaatttgct gcattgaata
    10741 tcaaaaccgg aacaagacaa gaagttgctt cagctgccgc agaacatcat atcagtgcag
    10801 accttgctta tgccgttcaa gagtatgtcc aagcaacaga ggatcaatcg tttttagaaa
    10861 caaagggaaa aacgctcatt catgaaacag cagctttttg gctgagcaga gctgtaaaag
    10921 tcgatgatca tttagagata cacagagtca ttggtccaga tgaatacaca gaatatatcg
    10981 acaataatgc atatacgaac tatctggcgc attataatgt ggccttagct gttaaatatc
    11041 aagctggcga tccgcttttt ttgaaagaat gtcaaaattt tttagataag ttatatttgc
    11101 cagtgcctaa caaagaacag ctgattccgc aagatgacac gtatctcagt aagccagtta
    11161 ttgatttgac cacttacaaa gaacgtcctg gcagccagtc tattttactt gattatgccc
    11221 gtagcgaagt aaatgaactc caagtcagca agcaagcaga tcttgtcatg ctgttatact
    11281 tattacctga gtatttttct caaccaatcg tttctaagaa tttgcattac tatgaaaaca
    11341 aaacaatcca cgactcatcg ttgagtaaag cgattcacgc catagaggct tgccgtatcc
    11401 aagaaatgga atgggcgtat catttattcc aagaagcgtg cctgattgat ttaggaccag
    11461 atccgcattc atcagatgac ggaattcatg ctgcttcatt aggagcacta tggctagcag
    11521 ttatatttgg tttttcaggt attacagtaa cgaatggaac tctttctatt tctccaagac
    11581 ttccttcagc atgggaaaaa atttcttttc cttttatttg gaaaaatcac aggttgctat
    11641 ttgttttgac aaaaaaacaa atagtaataa aaaaagagac gaaagaaaca ttacctattt
    11701 ttgtcaatgg agaccaatac cagctggaaa aagaactgat cttggaacgg aggggaaaat
    11761 aatgcgtatt ctgattgcgc cagattcttt caaagaaagt ctgacagcct tagaggcagc
    11821 acaagccatt caatcaggtt ttcaagaagt ttttgttcag gctgaatatg atctgctacc
    11881 tataggcgat ggaggagaag gaacggtttc atcattagca gacagtttaa ggttgaagcg
    11941 gcaaacagca gtcgttacta atgcatttgg caaaaacaaa gaaatccagt acgcttcaga
    12001 cggtcaaact gcgatttttg aaatggcaga aatcgtcgga ttgaaagatg tcccactaaa
    12061 ccaacaacag ccacttaggt tatccacgcg aggagtagga gaactgatac gacaattgtt
    12121 gcaacaaggt gttcgacaca tcatcatcgg cgttggcgga agtagtacca acgatggcgg
    12181 catcggcatg gcagaaggtc taggttatgc tttttacgat caagataatc aaaaagtaga
    12241 agcactggga gaaaatctag gtaaaattgc tacagttgat aaaacgaacg tgcctattga
    12301 gctgattaaa gccaaaatca ctgtcattac tgatgttact aatcttttat gcggaaaaga
    12361 aggcgcttct tttgttttcg gaccacaaaa aggcttgaaa gaaaatgaat tagcagtggc
    12421 tgaccaagcc atgcatgatt tttatatgag atttgcccct caagtaatcg aggcatcggg
    12481 cagtagtgct ggtggtggaa tgggtgcagg actgttagca tttgcttcag ggaaaatagt
    12541 ctctggcatc gactacgtat tggatttact ggagttcgat aaacgggtac agcaagcaga
    12601 cctcgtaatc gtaggcgaag gacgtctaga tggacaaagt ctaaaaggaa aagcgccagt
    12661 tgggattgct agaagagtac cagagggcgt tcctgtgata gctatttgcg gaagtgttgg
    12721 taaaggaagc gaaaaagcag cagatgttgg catttcggcc gtttttccga tccttcctgc
    12781 agctgtgcct ttacaagaag ctttacagca agccactgat aatcttcatc gcacagctaa
    12841 aaacatcgca tctttacttc aagtcgttca ttttcaagga ggtaatcgat gaaaatcaaa
    12901 gcagcactat tcgatttaga tggcgttctt gtagacacag ctcgttatca ttacgaagcg
    12961 tggctggttt tagctaacca gctgtccatt ccattcactg agaaagaaaa cgagcaactg
    13021 aaaggaatat ccagaaccga gtcactagag cgattgctgt catttggaaa aatggaacag
    13081 aaattctcag aaaaagagaa atcagccttt gctgagcaaa aaaacaatct atatcttcaa
    13141 gcgattcaaa aaatggatga aacatccgtt ttgcctggtg caatagctgt tcttgagtac
    13201 ttgaaaaaaa caaacatcaa gatcggattg ggatcagcaa gcaaaaatgc acgcctcatt
    13261 ttagaaaaaa cgaatttgac aagttatttt gatgtcctta ttgacggcac gcaagtatca
    13321 aaagcaaaac ctgatccaga agtttttctc aaaggagcac agcaattgaa tgtaccgcca
    13381 aatgcatgct tggtcattga agattcagaa gctggttgtc aagcagcttt ggctggtaac
    13441 atgcatgtat tgggaatagg tgaaaacata aatcttcctt ctgctgaata tgtgattcct
    13501 gatttaaccg tttttgatca agtacgttcg ttttggcatt tatcagaagc cgtatcacgt
    13561 atataatagt ttccgttttt tctgtcacaa gcaaaacgtt atcctccttt tggaaatcaa
    13621 ggtatgatga gaaaaaaggg ggattttata tgacaaaaaa agcagttttc gcaggcggat
    13681 gcttttggtg tatggtcaaa ccatttgaag agcagccagg gatcatttct gtgacatccg
    13741 gttatactgg cgggcatgta ccaaatccaa cttatgaaca agtaacaaca ggcacaacag
    13801 ggcatacaga agcagtagag atcgaatatg atccagaagt tatttcttat gatcagttgg
    13861 tggaaatcta ttggcagcaa accgatccga ctgacgcatt aggacagttc gctgatcgcg
    13921 gtgattctta tcgtccagtg atctactata atgatgaaga agaacgaaat attgcagagc
    13981 gttcaaaaca agcattgcaa gacagcgggc gattcgataa gccaatcgtg acaaaaatcg
    14041 aaccaagaag cgaattttat cctgcagaag attaccatca ggattattac aagaaaaaca
    14101 agctccatta caatctttat cgagaaggtt ctggacgtgc aggtttcctt cgtcgacatt
    14161 ggggcgtcaa gaaataagaa gccgaaaaag ctacttatgg aacaccgtat atcagaggtt
    14221 gtaaaaccag cattttgagc taatactgag ggtcagctga ttgaacacca tttagtggag
    14281 aggttgtgaa cctgtcgcct agctccaagt cacaaggatt tggtaagaga acactatata
    14341 tcagaggttg tgacaagtcg tttgtcacaa ccttttttca atacctaaga tgaaggacaa
    14401 tcagcaacgc actgctcaaa aaaggaatga aaggcagctt ttccaacttt tttttatgac
    14461 agataaagta aacagaaaaa acggatattc caaacagact agcaagaaac aagatctgaa
    14521 gcaactcttc tagagacaag ccaccactcc aagaaagcat caacagagta tcgccagctc
    14581 cgagatagga ttgaccatag cgaaggcaca caccgcctac gataaacgtt aacagcacac
    14641 tttgccattg gaagacgccg gcctgcaacc aaaaaatcca taaaattgcc caagaacaga
    14701 atagcgtgtg agaatggacc atgaagtaaa ataaatccat caacgaaaat agcaaagccg
    14761 aaagcatcca tatgaacgtc gaaaaatttc ttgcggaaga aaaagtaaag aaccaggcaa
    14821 aaagaccacc acataaggct tctgccaaca aatagtagaa gggcaaccga cacctgcaat
    14881 agcggcagcg aaatcgttgg acgatgatcg agagaatcgg aatcaattca taccaagaaa
    14941 gcgaacgaca gcatttgaca caatgagatc taggataaat aatagagtga cctaacggaa
    15001 tccgctgggc ggttaaacat aaaaatgatc cgatacagca accaatgata aaataaagta
    15061 acacgccatc tccccctttt caaaaccaag atacgcaagc ttaaactaaa tttttgtaca
    15121 tacataaaat ttgaggtgaa aaaatgaacg tttcaatcgg attagatatc ggaaccacac
    15181 aaaccaaagc agttgctttc acagatgaag cccaagaagc agcatccgct tattttcgct
    15241 accctttgat ccaagaaaca caagggatgg cagaacaaga tcaggaattg atctttcaag
    15301 gtgtctgcac cgttataaaa gaagtaatta aacagttgcc ttcagacaca atcattcaat
    15361 ttgtctcctt ctcctctgcg atgcatggcc tgatattgtt ggatgaagag aaaacaccgt
    15421 tatctcgaat gatcacttgg gcagataacc gagcagcgga cgaagcagaa aaactgaaat
    15481 ataccgtgct tggcaaagaa ttttatcggc agactgggac gccgattcat ccaatgacac
    15541 cattgaacaa aatcaagtgg ctgcaaaaca gccaacctga attattcaaa aaaactgctt
    15601 attttgtcgg tatcaaagac tatatctttt atagattgtt tggcgaactg atcagcgact
    15661 acggaacagc ttcaggaact ggatattttg atatccatga attccaatgg gcagaagaag
    15721 tgcttgacta cttgaacatt cgaatagagc aattgccgca agtctttcct tccacctatc
    15781 aagtaacagg tctaccatca aaaacagcag cagaactagg tttgccaaat gatatatcat
    15841 ttgttttggg aggggcagac gggccgcttt ctaatctagg gttaggtgct tttggtgata
    15901 cgatcgccgc tttgaccgta ggaacgagcg gagcacttcg ttttattact actcagcctc
    15961 agttccatcc agaaatggag acgttttgtt atgttctgga tcagaaacat tgggtagtgg
    16021 gaggagcgac tagcaatggt gctggcatct ttgattgggc agggcataca tttttacagg
    16081 aagtcgttca atctgctaaa gaaaaaggag aaaatccata tgatgcactg ctttcggcta
    16141 tcgcttttgt gccagcgggt gccaatggtc tgatttttca tccctattta cttggtgagc
    16201 gagcaccgct ttgggacgca gatgcgtttg gcagttttat cggtttacag cggcaacata
    16261 cagaaaaaga aatgatgaag gcgattctag aagggatctg tctcaacctt tatcggattt
    16321 taaaaggagt ttgccaggaa aatcagctca cagagatccg tgctacaggc gggtttgctc
    16381 aatctccgat ttttcgtcag atgatggctg atgtactggg ttacccgctt gttttcccta
    16441 ctgtcacaga agcatccgca ttaggagctg tgattttagg ctggagaagc atcggaaaga
    16501 tccaaagtat cagtgaagca caggaattga tagaattgaa agagcaagtt ggagtacaag
    16561 aaactgatca taaaaaatat cagaaaatct atccattatt tgtttcgaca caaaagcatt
    16621 tagcacagac ttatcagcaa tttgctcaac tgcgagaaga attagctgat tctgaaaaat
    16681 gattggcttt acggataaaa aagcgaactg aaaatcgttc tcaggtaaat gattttgttg
    16741 tacttttcat tgaaagtcta ctattatttt aagtgtaaga aaatgttagg aggcaagaaa
    16801 gatgaaattc caacaaacca aagaagtatt aaatcaatta gttgcagatc taagccaaat
    16861 gtctgttgtt gttcaccaaa cacattggta catgagagga ccaggtttcc ttacattgca
    16921 cccaatgatg gatgagttca tggatgattt gaacgctcag ttagatgaag tatccgaacg
    16981 cttgatcaca ttagacggtt caccttattc tacattgcgt gaatttgcag ataacacaaa
    17041 aatccctgat gaaattggcc gctgggatcg tactattcca gaacgtttgg aaacacttgt
    17101 tgcaggctat cgttatctag cagatcttta ccaaaaagga atcgaagttt ctggagaaga
    17161 aggcgacgat ccaacacaag atatctttat cgaattcaaa acagccacag aaaaacgtat
    17221 ctggatgatc caagctcatt tgggcaaagc gccagagatc gacgcgtaaa aactgttgtt
    17281 gaacttgacc gaaaaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa
    17341 acgtcaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat
    17401 tgaacaccgt ttaccaagag gttgtaagcc tgccgttcag ctccgagtca caagaaacaa
    17461 ttttaaaaaa cagcttctta tgttttatgg atattgtgac ttgtgaccga ggagttggca
    17521 ggagaacacc gtttattcaa aataaggggt tgtgacaaga cttttgtcac agcctctttt
    17581 tgtttttcat tttgacagga aacgaccctt ttatataaaa gaaagaaaag aagtaaagtt
    17641 tcatataaat ttttcaatca agcagaaaaa cgttgacatt gactagatta gcgggtaata
    17701 tgttaaatgg tattgtttgc cccacaaaga gccccggaaa ctcgaagtgt atgtcaaaca
    17761 tcagaactta aattggagga aaataacgtg cgtacaacat atatggccaa accaggcgaa
    17821 gtagaacgta aatggtatgt agtagacgca actgatgttc cattgggacg tctttcaaca
    17881 gttgttgcat ctgtactacg tggtaaaaat aaaccaactt tcacacctca tttggatact
    17941 ggcgatttcg tcatcgtcat caatgcagac aaagtgaaat taacaggtaa aaaagcgact
    18001 gacaaaattt attaccgtca cagcaactac ccaggaggat tgaaatcaat cactgctggc
    18061 gaattgcgtg ctaaaaattc tcgtcgtttg atcgaaactt ctgtaaaagg tatgcttcct
    18121 aaaaacactt taggccgcaa acaatttaca aaattgaacg tatacggtgg agcagaacat
    18181 ccacatgcag cacaacaacc agaagtttta gacatcacaa acttaatcta ataggaggga
    18241 atttcattgg cacaagttca atatatcggc acaggccgtc gtaaaaacgc agttgctcgc
    18301 gtacgtctag taccaggaac tggcaaaatt actgtaaata aaaaagatgt tgaagaatac
    18361 attccacatg ctgacttgcg tgaagtaatc aatcaacctt tcgctgttac tgaaacaaaa
    18421 ggcgcatatg acgtatttgt taacgtaaac ggcggaggat acgctggaca atcaggagct
    18481 atccgtcatg gtatcgctcg tgcattgcta gaagttgatc ctgacttccg tttagctctt
    18541 aaacgtgctg gcttacttac tcgtgacgca cgtatggtcg aacgtaaaaa accaggtctt
    18601 aagaaagcac gtaaagcttc acaattctca aaacgttaat atcctttata tatcaacgtt
    18661 tctggcactt ttcaat
//
LOCUS       DANJMI010000054        17674 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000054 DANJMI010000000
VERSION     DANJMI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.054"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(<1..199)
                     /locus_tag="PWU81_002298"
     CDS             complement(<1..199)
                     /locus_tag="PWU81_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110969.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
     gene            complement(362..1996)
                     /locus_tag="PWU81_002299"
     CDS             complement(362..1996)
                     /locus_tag="PWU81_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1110970.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            complement(2000..3373)
                     /locus_tag="PWU81_002300"
     CDS             complement(2000..3373)
                     /locus_tag="PWU81_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1110971.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            complement(3389..3628)
                     /locus_tag="PWU81_002301"
     CDS             complement(3389..3628)
                     /locus_tag="PWU81_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110972.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            complement(3810..6485)
                     /locus_tag="PWU81_002302"
     CDS             complement(3810..6485)
                     /locus_tag="PWU81_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HDL1110973.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            complement(6668..7591)
                     /locus_tag="PWU81_002303"
     CDS             complement(6668..7591)
                     /locus_tag="PWU81_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110974.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
     gene            complement(8114..9118)
                     /locus_tag="PWU81_002304"
     CDS             complement(8114..9118)
                     /locus_tag="PWU81_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL1110975.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
     gene            complement(9189..9485)
                     /locus_tag="PWU81_002305"
     CDS             complement(9189..9485)
                     /locus_tag="PWU81_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110976.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            complement(9478..9771)
                     /locus_tag="PWU81_002306"
     CDS             complement(9478..9771)
                     /locus_tag="PWU81_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1110977.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(9917..10468)
                     /locus_tag="PWU81_002307"
     CDS             complement(9917..10468)
                     /locus_tag="PWU81_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HDL1110978.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(10642..10941)
                     /locus_tag="PWU81_002308"
     CDS             complement(10642..10941)
                     /locus_tag="PWU81_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HDL1110979.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            complement(10968..11276)
                     /locus_tag="PWU81_002309"
     CDS             complement(10968..11276)
                     /locus_tag="PWU81_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL1110980.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            complement(11393..12352)
                     /locus_tag="PWU81_002310"
     CDS             complement(11393..12352)
                     /locus_tag="PWU81_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL1110981.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            12496..13272
                     /locus_tag="PWU81_002311"
     CDS             12496..13272
                     /locus_tag="PWU81_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1110982.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            complement(13370..14017)
                     /locus_tag="PWU81_002312"
     CDS             complement(13370..14017)
                     /locus_tag="PWU81_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL1110983.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(14017..14847)
                     /locus_tag="PWU81_002313"
     CDS             complement(14017..14847)
                     /locus_tag="PWU81_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL1110984.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            complement(14847..15596)
                     /locus_tag="PWU81_002314"
     CDS             complement(14847..15596)
                     /locus_tag="PWU81_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL1110985.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            complement(15610..16074)
                     /locus_tag="PWU81_002315"
     CDS             complement(15610..16074)
                     /locus_tag="PWU81_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL1110986.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            complement(16124..16585)
                     /locus_tag="PWU81_002316"
     CDS             complement(16124..16585)
                     /locus_tag="PWU81_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL1110987.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            complement(16567..17556)
                     /locus_tag="PWU81_002317"
     CDS             complement(16567..17556)
                     /locus_tag="PWU81_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL1110988.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
BASE COUNT         5413 a         3377 c         2581 g         6303 t
ORIGIN      
        1 tataataaac atttccttga ggtttccttt taatcccctt cggaaaatca ccttttgtaa
       61 aatcgctctt aaggaaatca tctaaattac cagttctggt ctttttacct ccagtattcc
      121 ctgatttttt aggtggtaca tgttctttag taagttcagt gtatttttca cataaaatac
      181 acttacccat ctttttcata tctatcccca ctctttttaa ttcttatata caattatatt
      241 tcaaatattc attaataaat atccctattt atgataagga cctccctaac tacttctcta
      301 acgatttcaa atcgctagag aagcgttagc gcccctctaa cgttgtttga tataacctag
      361 actagatcgt taacgtaaag ggtgttttta acacaaaaat agccgtatcc tttgttttgg
      421 atacggctat ttgatattgc tcattcaatt gttgtaccac ggcatgcgtg aatttcatcg
      481 taatttccaa catctcctta tggttaggat caaaggttat tttctctata aaggtacgat
      541 acaaggcttt gatttgcttc ttctcactct gggcaagaaa acgatccaaa ctagtcaata
      601 tccgttggac atctttgact tgaatcggct ctccttgttg aaagatccct aagatttcat
      661 tttctcgaat gtgcaactgt cgtcgttttt cagtcaattc gtctaagcgg tcttttaaca
      721 tcggaaacaa ttcaggacta tcttccaaag cagcttccca tttttcaatt ttagtcttca
      781 attcttcctt tcgttccagt aacaccacga gttcttgttc caatggttgg ctttgtcgca
      841 ctatttcttc atttaacgct gccaccaatc ggggaagaac ttctggtagc tgaacgatct
      901 cctttaatcg gtcggcgaca aatgtttccg cttgttcggc acgaatcgaa ttggcatgac
      961 acacacttgc tcctttattc cgaaagactg aacaagaata atatcgaatc cgtttctttg
     1021 tgccatcctt tagggtattt gtgacattac tcgctgccat cggtgcgcca cattctggac
     1081 aacgtaacaa gcctgtcaat acattttcac ctgtatggtt ccatttgggt tgttttcctt
     1141 ctaaagcaag gcgttcttgt actttttgat aatcttcttc cgaaataatc ggttcatggg
     1201 ttcctttcac taaaatcggt ctcggattct tccccttccg tcgtttggtt tcccaattga
     1261 tgtagcgcgc atattctaaa taccctgcat aaattttatt gcgtaagata tctttgactg
     1321 ccgtggtaga aaaagcattc tttttgactg tttgatatcc ttgacgattc aaaaaattgg
     1381 caatcgctcg ataccctgct ccgcctaaat attttgcata gatcagacga acaattttcg
     1441 cctcttctgg ttcaatgacc aattgttttt gaccattcgt gtcaatacca ctgacatagc
     1501 ctaaacgtcg gcctcctgca taacgctttt tatctttcac taagctttgc attgacattt
     1561 gaacattctc cgcaatttgg ttgcgttcaa attcaccgaa actcccaagg agttgtaaca
     1621 tcaatttacc tgtcgaagtg gtcacgtcaa attgttccga gatcgaatgt aatcctacgt
     1681 catatcgcaa taattcctcg actgtttgta agacatctaa catattgcgt ccgagacggg
     1741 tcaatttcca aatcactagt agatcaaaac gatggttccg agcatcgttc atcattcgtt
     1801 gataggcgaa ccgatttttt gtggatttcc cactgattcc ttcatcggca taaacttcta
     1861 ccacttcata gcccatccgt tcggcaaact gtcgtccatt ttgttcttgt gcttgaatac
     1921 tatgtccttc taaagcttgt tcttgtgtac tcacgcgaat atacaatgcg actcgtttgg
     1981 tttgtatcat ttccatagat cattcgttcc tttctaaaat attttgtgtc agttgtcgga
     2041 agtaaatccc acaaactgtt ttatcctttg ttacctcaat ccgacaaaca tagtcacata
     2101 ataattgtcg actagagagg tcttttagtt gaagccattc tttcactttt gcttcacact
     2161 gttggagtga aagaaacgaa gaagtagaca tcggcttttg aagagcttgc agttgttgct
     2221 tgagttcttc cagtgaacaa ttgccctctt caaatgcctt aatccattgt gcttcttgtt
     2281 tcttttgttg gattactttg cgtttttcat cttttcgctt ttcggctaat tgttgcgtta
     2341 attgggcaat caaggcttga tagacgcctt cgtattgaaa aaaagcctgt atcttttcca
     2401 atacttctga ctcaaccctt tctgcccgaa gaaaaattcc ttggcagctt cggtcggtac
     2461 aatgataata atgaagcgtc ttcgtcttct ttgtattcca ttgatacttc gaactcagat
     2521 gacgaccaca gaagggacac cgaatttttt gacgtaataa gtacagatac gtatcttttt
     2581 tagctgtaca ccttgattgg cgaatctctt gggcttgtaa aaaggttgct ttcgagagaa
     2641 tcggttcaaa ggttcctaga tacttcccca aagatcctcc tttgatttcg ccatagtaca
     2701 atggattttt taggatacta taaatatgat agggctgaaa aggacgttcg cggaagaaaa
     2761 gcttttgttg cgtcaatcgt tgacaaagct ttttgtagcc atattttttg gtcaaataaa
     2821 gttgaaaaat atagcgaaca atccttgctt cgtgtacttc ttgttggatt ttcccattca
     2881 ctaaacgata cccatatggt accgcagaag acaaatattg ccgttgttgc tttttatagg
     2941 aaagtccggc acgaacattc tcaataatga cgtttcgttg ttgttctgca aaagtgaaca
     3001 ataattgcac aaatagttta ttcgttgacg cgtgtgagaa agaagcagga agaggttcag
     3061 aaatcgaata cacttgaacc tgatgttgtt ggcacaactc aataaattct aaggctattt
     3121 tggcttgacg tgccaatcga tcataacgcc aaacatagat caccccctct tcttctgacg
     3181 cacaaaccca agctctaagc tgtcgaaaag ctggacgctc cgctttttcc cctgattctc
     3241 ctttgtctga aaatcgttgg ggaatttgtt catatttctc taaaacatac tctttcactg
     3301 catcatattg agcgtcatta ctaattttcg cttcttcata aacagacgtt cgatcataac
     3361 aagccacacg catgcttgat tcccctctct atgaatgaac taaataccat tttttcacga
     3421 ctcttctatt gaacgaacgt tgcacaaagt gattttctaa atatccttcg ttcttcaacg
     3481 cttgaagagt acccccgata gatccagcag atacccctct tatttcgaaa tccaagtaaa
     3541 ttggttttgt aaaaaaggct tgatttgaaa aatgttcttg aatatacaga agaacctctg
     3601 cttttagtgt cattttattt ttagtcatta gaattttcct ttctttcatg tttgattgaa
     3661 tgaagtaaat gaagcataga tcaagcttca tttacttccg tttgggtttg ctcgctatca
     3721 tacagataag taatcagttg ttttgctaaa tcaataagaa atatttccat aacatggtgc
     3781 ctcactttca tactttggtt tattctaaat tacaatcgaa aatacgattt gagtgaagaa
     3841 ggtaatagaa tctcgtatgt tggaagaact tgacgattca tgtgaatacg tttcgttctt
     3901 ctcccttttt ctgcacacat attacccgaa tcaagcaatt gcctaaaaag ataggttaga
     3961 tcctttactt gaaattctcg ccttaacaag tcctcaaacg ggataggaaa aatatttacc
     4021 ttaatatccc catttttttg aattgatact tttccccaaa tcgtactggg cgtatgtttg
     4081 ttgtcactgt ttgttaaaaa tttatggcta ttcatcacca acaatgtttt cactcggtca
     4141 aataccgttt gctcacttcc taaactagta tctacaatta actgttcgta cgaattaata
     4201 agcaccgtct gtatatgctc taatcgtaaa ggatagccca gtatatcttg aattaggagg
     4261 gctccactat aaatcaaagc tagattatta ccaatgcgtt gcttcaaaga atgttcaggt
     4321 aaaacagccc atagtttttt ttgagcttcg acaaaagttg cttcaatttt tgtaatccct
     4381 tgttcaaaga gtttcaaaag aaataacttt ataccccaac caaaagtatt agaagaaaga
     4441 cttttaactt tctctgcgtg attcgcatcc tttgtaaact tgatatcaga aaattcgata
     4501 tatcgaactt tcaaaccagt taatttctca ttgaaataat tttccaaacg ctcttcagaa
     4561 gaggtaaggg tcaaaacaga aaaagtctct tgtggcataa gctgacaatt agaatctaat
     4621 cttagccttt cttctcctga agctaattga tagatcaacg agctaaagtt attttgagtt
     4681 gctgctccta actcatctag tattagtgga acacctccct gattgtttga tgctagcttt
     4741 ataatggctt tattggtagc attcatattt ctaagaaccg tagttggatt tccaaataaa
     4801 gaggcaataa agtgtaacgt agtggtttta cccgttgtgc tcttgccctg taggttaatt
     4861 agtaaactca aaaaagtatt agccttcgta acgtttaaat agcctaacac aatagaagca
     4921 ataccaatcg accaagcaag ttctaagttc gttcctaaaa catacttatg aaccaatgcg
     4981 tcaaattccg cttctgtacc attagttgtt aattggtagc atgagttttt agttaataca
     5041 gaaggaattt cacgtccaaa agaatcttgt aactgaaaaa tcattgcttc attttctttt
     5101 ttccagccaa gagattcatg ttcaaaaaag attggagcat tctcccgttg aatctgtaaa
     5161 aagtcaacga cttcagcttc tcttctagct ttaatgatta agccatgttt tgctaattgt
     5221 ttaaaattat tagtttgtaa acatccacct tctatttcta tttcatccca acaatgatcg
     5281 acataatagg caagaacaag tgtaatgact ttttgttcga tatttttgcg tatttgctta
     5341 ataaacatcg gacaagcaac tttttcttct tgaccttctt tcaccaaaaa tactccttct
     5401 tgattcagtt taaactggtc aaaataatat tcttgattgg attctaatga ttctacagaa
     5461 ggatttacag gttttggcaa cggatatttt tttacaaagt cttgaatatc tttattttta
     5521 tccgaagcat gcagacaatg aaatcgaatc ttattatcag ccgtaacaga caaacaagaa
     5581 ccattagaat tattcgtgtg ttttcttaat ggacattcgt caaatataag taaggtaact
     5641 cctttatgat cgccctcttt tatggtataa gttaattcag gatagtgagc taaccattgt
     5701 ttatagtctg cttgaataaa aggtaacact tctctaaaag atggatggct ttttactcta
     5761 gaggttgaca tcatggaatt ttcttcaact atctgttcaa gaattttaaa acaattactt
     5821 ctatctatgt tatcgggaat agaaaggaaa ctagataaac gatgcggacg ctcttttgtg
     5881 gactttcctt tgaccgctgg tgtaccaact aatttgcata atcttgaagg attataaaca
     5941 gaagtatcaa tatcaacttc atcttctact ccaaatcttc ggtgaagtaa ttgaagaaaa
     6001 ttctgaatag taagaatcgt ctcgtgatta tttggttgtt catttatggg aagaaaaata
     6061 tggtagccat tcccactatc aacttttaca aatgattcta ttctcttttc tttcaaataa
     6121 cgttctactt gttccataac attttctgcc tttttctttt cgctatctgt tgcacttccc
     6181 tctttccttt tagggtcaat atcaataaat acaaaccgaa ttttttcaat atcattgtct
     6241 ctcacagaat gctttttttg tgtaatcacc tcttgtttaa taggattata agttatgttt
     6301 actgtacctt cagacaattt ctgtttttct aaatagttaa taattgcttg agaatctgca
     6361 gtagaaaaaa tatgagaaac ggttttcttg tttaatgtag aaaaagttct caaattaaca
     6421 taagaaatcc ccagtgtatt taacagttgt aaatgcttct gaattgaagt gtgattcatt
     6481 ttcattaatt accactcact ttctaaaaaa tttttatttg tatgactaca ttacctccaa
     6541 cctctcataa tttcatgatc ttcagttcta caaaaatata tcccactcaa aatcttgaca
     6601 aaagaaaaaa gttgtgattt tggaaatcac aacttttttc ttttacgata aatcaatcct
     6661 tattaaatta tttaggaaaa tattgttcaa ttatttcttt atatattttc tcatagtatg
     6721 tttctgtcca tcctaatgtt cctataaaat caagtcctct acattttaaa ggttcactta
     6781 aactattaat atagttaata atgttgtcta tccttttctc tttatcttca ccttcaatct
     6841 ggggagcata tagtccaatt tcctcccaat aatatgaatc tctttcaatt cccatgatgg
     6901 tagctttggc taatttcaaa tcaaataacg attttagaat tcttagtgtc tcaataaagt
     6961 cttctctctt caactcttta atccattgtg aattttctgg attatctaaa atttcaagtt
     7021 catcttcatt ataagtatat ttcaaatttg acttgcttaa ctcatagata aatgaagttt
     7081 cttgactcat aattctttta agtacttgac tataagtttc gtttgacttt gcccatttcg
     7141 ctaattctac cactgtattc actaataaat cgaaataatg atgttctcgt tcaatagtct
     7201 cagcattttg aaaatgaaaa tcacctttat aaactccatt cttttccact gttttattaa
     7261 tattttttaa aaaccagaat gagtttgcaa atttagtcgt actttttcga ttaactaagt
     7321 taataactct tccatgtaat aataagacaa aaatacataa atttttagct tccttattat
     7381 tagatactaa ttgatcaatc atagggagcc attttttgat tttcactaat tgattgctca
     7441 taaatctttc actggacttt tgttcaatct taaattgcaa aacttctaac tcataattcc
     7501 catcttctga tttatttttg ggaacaacta tcaaactttt ataaagttta tccacatcaa
     7561 aatttatctt ttttgcataa tttgtagcca ttaatacatc tccttattca taataaattt
     7621 catatgacgc tcctctcaaa atttaccggt agactagaaa aaagaataaa agatacgtca
     7681 attcacactt tattcttaaa aaaaaactgg tctctctacc ggtaatatcg attgagtccg
     7741 cctttatcga ctttatcctt tttttatatt aaattttttc accggtagac agggagagaa
     7801 agaataaact aaaaagccct taattcttaa tgttttcacc tccttaccgt tcctaccgct
     7861 aaaatttaat ataactaaat tctataacat tagaagaaat tttaaatacc tttgataaaa
     7921 aatattgatg ttttttctct cattgtttac aaactagata gcttatgtat tcttattaaa
     7981 aataatagat atttcgaaca tcatcactct catctactta atttcgcatt tttactttat
     8041 aaaaaaactg attctaagaa aattaaataa tttttctcaa aatcagtttt tctttgataa
     8101 ataatatttt caattattta atcatttgaa aattgttagt cattccaata cgttgagaaa
     8161 ttcttccatc tgtataaaca cggaattgtt ttccaccagc cattgttgta tttccattat
     8221 catccgttac aatattaatt gaatagtagc ttccattttc atcttgcatt agatctttca
     8281 tatattcatt tgtgctaatt aaattagtat ttgatgcatt tttatcagga gaatcataca
     8341 accataaagc tttattattt agtacagttt ctttagcagc ttcccaactt gaaattgctg
     8401 agctacttgt agattgtgtc gttgtagttg ttgcttgttg agtagattct tctactactg
     8461 gagtttgagg agtttgtcca ttcacaatat caatgaaacc ttgttttaac tgttggttct
     8521 gagtttcatt gaagtacatg tagtttttat cattgccttg attttgttgc gttaaataaa
     8581 ctttaggttg tccattttga aagccaaaga aatatacatg ctgccctaaa tattctccag
     8641 tttctacatc agaataaact gctactaaat tgaaatctgc atgaccagtt ccatcttctg
     8701 accattgtac ggtaactggt gcttcattga ttgccatttt ccaatttcca tcaataactg
     8761 cacttggtaa aggcgtacca tacagactta cattgttctg aggattgtag cttttatatt
     8821 cttgacctaa tgtctttccc cattgtggga caaaaattgc tagttgagta gatttctcac
     8881 tatcccacaa agtattttta acctcttcag aagaagattc aatgattgaa ctttcacttg
     8941 tacttgcttc cacagattgt ttcatttctg ttgtagtttg ttcagttgat tgcgttttac
     9001 tttttttagc ctcaattgtt gtatcagttt ctacagtctc tttagtcctc gtattctcag
     9061 atgtacaacc agtgaataaa atccccattc caattagtaa taacaaccat tttttcattt
     9121 tatttccccc tttcaatttc tccctcgcgt acttatttta tcagtcaatt aatctcaatg
     9181 gtgttcattt agttaatcct ttcccaagaa tgtcgatagc cagcatgaca acgatctctt
     9241 ccttgagggc tttcagatgg attaaaacta gtagaaattt cccttccaca tcctctacag
     9301 cgataagttc tcattatttt tgggctatta gtattatttt tcttagttag agctatgagt
     9361 ccaactatga ttaccccaac tgtcttaatt acttttcctt tatctatatt cggatgcgca
     9421 agcatatatt tatataacca atatccaccg taaattaaac cacctaatac aatcagctta
     9481 gccatgtact cgtctccttt ctttttgttt aattactttt tgaatccatc ttgcagagaa
     9541 accataaact tctgtcaatt gacggatatc tacagttttt tcctgttcta tcatgttttc
     9601 gattcttttt ctaactaact cacgatcgta cattttcatt ggaaaagaaa tctgttgccc
     9661 tcgaaaagca agataaaatt gatagagcaa ttctgaatcc agttccattt cctcaaaaaa
     9721 gtcattcata cttttataag agttactcaa acaagtttca tcaatctcca tctacacacc
     9781 ccctgttatt ttttacataa tgaaaatagc atattactgg gtacattgat aattcatata
     9841 acatgtatca tcacatgtat ttttaaagtc aaaaagcatg taaaactact tataagtaac
     9901 ataagaaaat aaaatgttat ttttcttgaa ataataattg actatctgcc aattgccatt
     9961 cattacgaac atgaatactt agtgggtggg ataacttacc ttctgcattt atcaatactt
    10021 tggtcatctt tttttgtttc gcactcaatt gatcgtaaat ggtcttcgct tgttcataag
    10081 ccacttgact cgtcgctgta atcgaaccac aagcaaaaat aatggtgttg attttctttt
    10141 ctgtgaaaac agttgttaat atttccaaag attgttcatc atttccacga ggaaataccc
    10201 gaggactttt cgcttttgta tacgaaaaaa gattcacagc aataaaacca gtaaatccta
    10261 attgtcggat attcttttcg attaataaca tactcaaatc attcgtaaat ggttctgtat
    10321 ctgttggacg aatagtaatg actaccgcta atttatcttc cgcttctttt cctttgatcg
    10381 cccattgtcg tttgaaaaca tagcgatggg tatgatgagg atctcgccaa acttctgttt
    10441 gtgtggtcac taattctttt tcaatcatct ctttttctcc tatgctaatt tttgttcaag
    10501 caattcaatc accattgccg ttataagtgt caatactagt tgaaaatatt tcatattctt
    10561 tctccttttt tcttcagaat ttgatgaatc gctgtaagga tatcttctaa acatttcgca
    10621 atcattctca aataatcgat cttattcatt cgttcctcca atctgactcc aaaccaatcg
    10681 tccttctacc agtaacccac gaacaaattc tcgctggctg gtaatatctg taatgaaata
    10741 aactacatag tcttttccat cttctggtgc gcaataaata taaagatgtg tcatcactaa
    10801 acgtggtaga gtaacatctt caggaagcat acccatttca atttcctctt tttccatcgt
    10861 aacggattca tagccccact cttcaggagt agtgactagc tgtgcacact tttccagtac
    10921 ttcgatagcc gtttctaaca agtcctttcg ttcctttctt ttagatatca gtattcttct
    10981 ggtaataaca tcgtttgata ctcacccgaa tcaatgaccc atattttttg agacactgat
    11041 ttcaaacgaa gcttaggtga aatcagatac tcttttcttg cttctggttc ttcttgacga
    11101 tgaaccaact tttgatttcc tttttccgta gaaaattcaa agatttgaag atagtctaaa
    11161 tgaaattgct tttgaacgtt ttgatcgatt agattccaac ataaagcttg taattccacc
    11221 ggcaaactgt ccattactcc ctttgtgaca taacgttgtc ctttattttg aaacatttct
    11281 tttccctcct ttgccataaa aatagcccaa tcttctatta aagaagattg ggcttcatgg
    11341 atataatata tatttgatat tcaatttgat tcattttata ctatctgatt tgtcaaatta
    11401 agctagacaa tatgtcttta tctatgcagc tcaaaaatac ttccaatggt gttcgataat
    11461 ttaatgattt tcttggaata tggtttcttt tcgaggcaac ggattgaata aaggtttcat
    11521 ccgtgtccct aaagtccatt gttttaggta agccatctcg tctaagcaag ccgtttgagt
    11581 gctcatttag ccctctttgc gaaggtgttc ctggatctgc gaaaaagatg gcaatatcgt
    11641 tcgagttact tatagatcgc cagttggaaa attcttttcc acaatcaaat gtcatggatt
    11701 taaacaagtg tttaggcatg gatttgaacc aatcatttaa cctgttttcg atatcttcgg
    11761 ctcgtctgcc tgtcggtttc aaagtaataa taaccttgga taaacactca accaatgtaa
    11821 taacagcgct cttgtgttta actcccacaa tggtgtcgac ttcgagatga ccaaattctt
    11881 tagtcaggtg tggatagaga ctgttccgtt catgaatcgt tctgcgaaag gcctgttttc
    11941 cacgcttttc ttgatgatta ttcggtttac gtttcccctt cataggtaac gactgtgtgt
    12001 caaaaaggcc ttttttaaac atccgataga gtgtacgaac agaacaatca attggaaact
    12061 cgccacggcc gataatcaca tcgggtgacc atccttgagc taccttttct tgaacatatg
    12121 ttgattgttc atcagataaa acggttgaac gtcgtccaca acgagattta ttttgtttgt
    12181 attgacggta ataatctagc actttttttc cttgtttgag aaacgaataa acattataaa
    12241 tggtttgtct agagcgattt aaactttgtg cgacaagagc gacaggtttc tcgatattga
    12301 aataagactc tatgattact agttcgtctg ttgtaagatg ggtgtaagtc atttgtactc
    12361 actccttatt aattctttgg tcggaactat tttgagtgta gcacaaatga tctttttagt
    12421 tgtctagctt aattttacaa tcgacgtttt aataaattgt gatataatga agtataactt
    12481 tttagggagt gtactatgaa ttttcaagat aatagaccat tatatttaca agtagaagct
    12541 tctatacgta acgatattcg tgaaaaaaaa tatcttccag gagagcaatt acctacagaa
    12601 gaagaattat gtaaaattta tggagtaagt aagattacca ttcgaaaagc ttttaaactt
    12661 ttagcagaaa gtggaacagt tgaacgatta agggggaaag ggacctttgt aactcaaaaa
    12721 aaagaaagtt taaaattagc tggagttcat ggatttagcg attctctttc tacccgagga
    12781 cataaaatta gatatactgt tttacattct aaaattataa aggctgatga gtttttagct
    12841 aataaattac aaatcaatgt aactgataga gtttacaata ttaaacgttt aatgtgggaa
    12901 gacggtgccc cgatgggaat tgataatttt ttcgcatcag aagaaaaatt tccaacttta
    12961 tttgaaaaag gtagccaata tacttcatta tataaacttc tagaagatga ctatgatgtc
    13021 aaagctacga gctctattat ggaaataaac ggtttatctg caacacctga gctttccgac
    13081 ttattgcaat gtatagtagg agatccgcta tttattttag aaaaggttgg caaagataaa
    13141 aataatcaac catttcatta ttctcaatcc acagttcgtt gtgatagaat tagttatgtt
    13201 attgaagtca cagaaaatat cactttagat acggtgcata aaaatgattc agatcctaaa
    13261 gtaatagatt gatattaaaa gaaatcatac ccaaagcaat caattttgat tagatttggg
    13321 catgattttt atttttacta atattctcta aatacatcaa aaaacttatt tatattccaa
    13381 atttcacctg tacttcttgt cgcagatatt taatatcgta aataacatca tctgctagac
    13441 ttacatcttg agaaaaacgt ttatctttaa ttgcaagaac cttcatattt gctcttttac
    13501 cagcttttat accattataa gaatcttcta tcaccagaca ttcttcaact ggaattctga
    13561 gctgttctga agctttaagg aatatagtag gattaggttt actttctggc aaatctcttc
    13621 cgctaattaa acaatcaaaa tatgactcta ttttacaagt ggtaacaact tcatttatag
    13681 tatccattgg actggaagaa gcaattgcca tttttatttt tttctttttt aaccattcaa
    13741 gctgttcaat tagtatatta ggaaaaatat ccaagtaatc aatcttttgc gatttccaat
    13801 atttagtaaa ttttcccttc aattctaaac tgttacatcc ctcattgtac ttttctatat
    13861 catcaaaaat acttgctgta gttccaatct ttttttgata agaatacaaa ggatagctta
    13921 aattttcttg ttttaaaaaa ttaaataccc acttttgata cataatctcg ctatcaatta
    13981 aaactccgtc catatcaaaa attagagcct taatcattat aagataccta ttatacttaa
    14041 aacaaaccct gccaaaatac aacctaataa gagcatattt gtactaacat ttttcttaat
    14101 caatttatat attcccaaag tacatacaag tggtaataat ccaggaataa tggaatcaag
    14161 taagtcttgt aaaacaagtt ctgttccccc tgtagtgaat tttaaagggg tgacaattgg
    14221 caccatgcta gccaccattc cacctactac agccaaacca atgatagaag caaattgcat
    14281 tactcgatcc ataagacctt cttgatacaa tttagtaagg tatttattgc cagactcata
    14341 tcctaattta ccgccgtaaa aaacagctaa aatagaaggt atagcaaaaa ggccaacagc
    14401 taaaatcggt cctaaaatac taccttgctt agcaaaagac aatccaatcc caaatgtaat
    14461 aattcttaaa gtaccttgga aaaatgaatc accaattcca gcaaaaggac ccattaaact
    14521 ggttttaatc atggagatag attcagtatt aaagtttcct tcttctgctt gagcattttg
    14581 ttcttccata gaagcacaaa gtcccataat aaatgaagtc atttgaggcg tacagttaaa
    14641 aaatgccata tgacgagtat aagcttctct ttttttctct tcatcatttt tataaacttt
    14701 atttaaaatt ggcgccatcc catacataaa tcccatatgc atttgtcttt cataattcca
    14761 cgaacacatg attgtccatg aacgaagaca catcttatta agatcttttt ttgtgattaa
    14821 acgttttttt tctgtactat tttccattaa ccatgcgcct cttctctttt tccatattta
    14881 acttgatcca ttacaaaggc aatcagagct gcaaaaattg tcacgccaat tagtgaaaga
    14941 cctgaatagg caacaataaa gaatccaaaa ataaagtaag gatataattt tttactcaac
    15001 attgtgctta atagcattgc aaaccctaat gcggaaagca tatttccaga gacagtcaag
    15061 gcgtctgtaa tcatttctgg aattttatct actaaatctg ccacaacgtc agcaccaaaa
    15121 tatactgtta aaaagattgg aataaagtaa acaaaagcat taaaaataaa tgaccaataa
    15181 atatgaataa tttgagcttt tctaaattta ttattttcaa tggctttatc tgcagcatgt
    15241 ccaaattgag caattatcat gcgcatgata atactcataa attcaccagc tacagccact
    15301 gggatagcta ttgttaaggc tgtttctgta ctactattag ttaaaattgc aaaagcagtg
    15361 gcaataatac taccaagttg catattagga ggtccagccg ccccaattga gacgaatccc
    15421 atagaaatta attctaaaga tgccccaaga acaatccctg tatgcaagtc acctagcaca
    15481 agtccggcaa gtgtactcaa aattagtggt ctaccaatat tttgtcgacc aattaacctg
    15541 gagtcaataa cagctaccac accaactaaa cctaataaaa tggcttttaa taacatttat
    15601 tttcccccct tacttactaa aatctacatt ttcaatactt gaagatggga cttgttgcac
    15661 aaatattttc acttcttgat caagaatttt ttttgtggct tctaattctt caggagttaa
    15721 aaagactgcc tttccatatt gtttggactc gcttttctta gacacaccgc caaaattaat
    15781 ttctttaatt tctggtaaat tttgaactaa ttcaaaagct tccttaggtc ctttaattaa
    15841 aatcatgtaa ttatcatttg gggatgtttc cataatccct ttaacatcac taattttaac
    15901 aatgtctaat ttgcaatcag caggtttagc aattgtcaaa gccatcacag acattggatc
    15961 tcctggaact ttgttatcag ccacaataat atggttaata tcatactgtt tagtccaaga
    16021 aaatattact tgtccatgaa gtaatctgtg gtctacacgc actaatttaa tcattctatt
    16081 tcctccttca atttttaaaa tttatagaat agaaattgcc accttaactg acatctttta
    16141 aaagcgaatt aatatgaacc atactttttt gagagttatt cagtaattcc ttaaaatcta
    16201 tttcttctat agatttacca gctgactggg ccattaataa ctccagtaaa agaggtaaat
    16261 taatcccagc aataatttga attctattgt tgttcaactg tttaatagaa acattattag
    16321 gtgttcctcc aattaaatcc gttaaaataa ttagaggttg caaatgatgc tgactaatta
    16381 tttctttcat actctcctca aaatcatctg gactagtatt ttctttaaat ggaacagcac
    16441 taatacctgt ggcgtcattc attaaaatta attttgttgc ctttaaataa ccctcacaaa
    16501 gggtagaatg tgttgtaatc acaatgtgaa ataattgatt tgccatcttt tgcaacctcc
    16561 tattaattaa tatttcattt gccacatata gcgtctaaca tctaaggaat ggcctctttc
    16621 aaaagcaatc gcttctacca tttggcgaat aacaacgcca ctaagtacag ctgcaatata
    16681 ttctttcgct acactatcta tagattctaa gtcaaaatca gcttggtcta aaaccattaa
    16741 tttattactg aattttttag tgaagtcttc tacccgttga tctaaaaatc ttgtatctcc
    16801 aatacttttc actaagatga atggtacatc ataatcagtg atttcaaaag gaccatggaa
    16861 aaattcccca gaatgaatag cattagaatt aatccattgc atttccatga accagcaagc
    16921 tgctgtggag taaatttctc cgtaattgat accgctacca attgtgtaaa taattttatc
    16981 tcgtttttgg tttttacccc attctttagc agttatttca aatttgctct tttcagcaat
    17041 aacaagatca tttaacttat caaattcatt caagcaagtt tcccattttt tagagccatc
    17101 taaaacaagt aaaatattaa ataataaacc ataaagaatt cctttattta aatcactagc
    17161 atcaacatct ttaccccaat catagtgaac aggatattca gctgcttccc atagaggaga
    17221 tccttcttca ttagataaac aaatagtcat tgctccaaat tctcttgctt tttttgttgc
    17281 ttctacagtt tctggtgttg tgccagaatg ggaacaagtg ataactaaag aattttcaga
    17341 taatcctttt ggtatagcat agttaaattc gttagcagta taaacatgac aaggtacagt
    17401 taattcttta tcaaatagat attgaccagc cattaaaacg gcttgtgaac ccccacatgc
    17461 tgtaaaatat acatgatcaa ttgtcttttc ctttaacgct gccattgctg tttcaatctg
    17521 ttctttgtgt agttgtctca tttctttatc cctcactcta ataagtatta taaagtcata
    17581 taactttaca atacctatta tctatgaaat tgctttattt tgcaatacta tttttcaaaa
    17641 aaattttttt ataacaattt tatataataa aatg
//
LOCUS       DANJMI010000055        17008 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000055 DANJMI010000000
VERSION     DANJMI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.055"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(<1..127)
                     /locus_tag="PWU81_002318"
     CDS             complement(<1..127)
                     /locus_tag="PWU81_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HDL1110989.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(231..542)
                     /locus_tag="PWU81_002319"
     CDS             complement(231..542)
                     /locus_tag="PWU81_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL1110990.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(544..741)
                     /locus_tag="PWU81_002320"
     CDS             complement(544..741)
                     /locus_tag="PWU81_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HDL1110991.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1135..2862)
                     /locus_tag="PWU81_002321"
     CDS             complement(1135..2862)
                     /locus_tag="PWU81_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HDL1110992.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2855..4048)
                     /locus_tag="PWU81_002322"
     CDS             complement(2855..4048)
                     /locus_tag="PWU81_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL1110993.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4235..4879)
                     /locus_tag="PWU81_002323"
     CDS             complement(4235..4879)
                     /locus_tag="PWU81_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1110994.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4973..5107)
                     /locus_tag="PWU81_002324"
     CDS             complement(4973..5107)
                     /locus_tag="PWU81_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDL1110995.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="PWU81_002325"
     CDS             complement(5109..7241)
                     /gene="feoB"
                     /locus_tag="PWU81_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDL1110996.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7238..7711)
                     /locus_tag="PWU81_002326"
     CDS             complement(7238..7711)
                     /locus_tag="PWU81_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDL1110997.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8112..8336
                     /gene="nrdH"
                     /locus_tag="PWU81_002327"
     CDS             8112..8336
                     /gene="nrdH"
                     /locus_tag="PWU81_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDL1110998.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8495..10654
                     /gene="nrdE"
                     /locus_tag="PWU81_002328"
     CDS             8495..10654
                     /gene="nrdE"
                     /locus_tag="PWU81_002328"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDL1110999.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10704..11669
                     /gene="nrdF"
                     /locus_tag="PWU81_002329"
     CDS             10704..11669
                     /gene="nrdF"
                     /locus_tag="PWU81_002329"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDL1111000.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11758..12897)
                     /locus_tag="PWU81_002330"
     CDS             complement(11758..12897)
                     /locus_tag="PWU81_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL1111001.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13206..13919)
                     /locus_tag="PWU81_002331"
     CDS             complement(13206..13919)
                     /locus_tag="PWU81_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDL1111002.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="PWU81_002332"
     CDS             complement(14173..14874)
                     /gene="nagB"
                     /locus_tag="PWU81_002332"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL1111003.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15108..16640
                     /locus_tag="PWU81_002333"
     CDS             15108..16640
                     /locus_tag="PWU81_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL1111004.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16831..>17008)
                     /locus_tag="PWU81_002334"
     CDS             complement(16831..>17008)
                     /locus_tag="PWU81_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1111005.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5279 a         3495 c         2910 g         5324 t
ORIGIN      
        1 atgaaaaaac acattccact aaatttgatg ccggttttcc ttgaggtttt gttccagcac
       61 ctcctttaat ttttttaagc tcttgatctg aacattgttt agcatttagt ctattttttt
      121 cttccattac gattccccct acaaactata aatactttta tgtcactgat tcaatatacc
      181 tatttttcta atcaaaaaat tttgagacat atttaatatt ataagtatca ttaaaattga
      241 gatttatctc cataatctgc tcgatataaa ccatttattc cagcagttct tccaatttca
      301 ctcaaatttc ttattaattc ctcttgatat tcagtgaatc ttattttatt agtataagca
      361 gtaaaatata gataattcac caatctattt attaaaggac tgggatctaa ttcatatttt
      421 tgtttttcta attttaaata taccttttgt aaaacctcaa gaatatcaga atatttagga
      481 tctttactta tagatatatc attatataat tcatgaacaa tttgcttagc attttttttc
      541 attttagcac ttccctggaa ttgctccacc taaaaaacca cctatagaca ttcctgcaat
      601 acaagtagtt gccttggccc aatcgaccgt acatttattt ttagtgcaat acactccatt
      661 tccataatat tttccactat gagtggtacc cccatagata agttgtgtct ctttaataga
      721 caaaattttt aaatgtttca taatctaatc acccctaaat taatgttttt agactattat
      781 tctaacattc attaaatttc aaaaaacaat tccttcttga aacgtagcaa aataattcct
      841 gaataagaat ttaatcactt ttaagtatat aaactatgta tattctttaa tattttttga
      901 gaatgcactt cgtacataat aaactttaat cacattagtc tttcatacct acgatcgatc
      961 tctttgactc gcacccataa attcataata gtttgtcggc gtctttgaac gtttcgaaga
     1021 tgagctacgc attctctttt tttatagaca aaaaagatag agacgaacat ggagcttcca
     1081 aatttggata tttgatccaa ttttggaagc tccatttatt tcgtgtctgt ctttttattt
     1141 tttccagctt tcgactaatt cttggttctc tttgatccag tcttttgctg cttgctgtgg
     1201 atcttttcca ttgttgattt ccaacataac tttttccata tctttttctg accaatggaa
     1261 attgtctaat actttgtaag cttctggctg gtcttctttc aatccttttc gaaccatcgt
     1321 atggatctgc tcttcgcttc ccattccgtt ttcaggatct tctaagtatt tcagatcata
     1381 tttggcaaac atccaatgag gtgtccatcc tgtaactaca attggttcgt gttttttgat
     1441 cgcttgacct agtgcaacgg tcattgctcc tgaagaagat gtctctactt tccagtcttt
     1501 caagttgtca tatttttgga tcgtgttttc agcagccgta actacaccag ctcctggctc
     1561 gattcctatg attttcttac ctgcctgatc ggttaactca tcgatcgaat ttacatccat
     1621 gtaggctggc actgctaaac caacttttgc tcctgtcaaa ttggcaccta ggtcttcgac
     1681 ttgatcttta tattgtgcat attgtgaacc atgagtttta ggtagccaag ctgaaaccat
     1741 ggcatctgac tcgccatttg aaacagattt ccacatgatt gaattatcta atggcgtcat
     1801 cgttacatca tagcccattt gtttcaatac ttcaccgact acatttgttg aagcaacttc
     1861 tgtatcccat tcaacataag ataagttgat acgttttgtt tctttggcag atgaaaaact
     1921 ccctatacca atcgcaccta agatcactac tactacagca ccaatgatca gtccttgacg
     1981 ttttttactt ttctgtgttg ttgctcctgc agctttttta ttgtttggct ggttcaaatg
     2041 ctgtgtaaaa cggtcaatga tgattgctaa gataactaat gcaaccccgt tgacaaatcc
     2101 attcccgact tgtgctcttt gcaatgctga taatacgccg cgtcctaagc cgggtgcacc
     2161 gatcatagag gcaataacga ccattgaaag tgacagcatt gttgtttgat tgatccctgc
     2221 catgatcgta cttttagcta aaggcaattc tagtttgaat agtttttgac gacttgtgct
     2281 cccgaatgaa tcagaagctt ctactaattc tttaggaact tgtcgaatcc ctaaattcgt
     2341 aaaacgaacg gttggtggca gagcaaagat gactgaggca aatacccctg gaaccatccc
     2401 gataccgaaa aatgcaactg ctggaatcaa ataaacgaat cctggcattg tctgcataaa
     2461 gtcaaggatc ggtttgataa tactttgggc tttactgctt tttgccatca agatccctaa
     2521 tggtacccca atgatgatcg agatcacact agataaaaga acaagtgtga cagtattcat
     2581 caaatcattc catagacctt ggttataaat aaaccataga ccgattaatg tgaacaacgt
     2641 aagtcctgga cgtttcttag aaataaagaa agctgctacc gtcaataaca agataaacaa
     2701 aggagctgga atcactaaca gtaaatttgt aatccccgac atcactgcct gtccaatcgt
     2761 ttgtaaaaat gaaaataggc cagaaaaagt tgtcgtaaac cattctgtga tattttctac
     2821 ccagtttgcc actggtaatt gatagttcaa tagattattc atgttcactc acctcgcttg
     2881 tttctgctaa tgcttcaatc acactacctc ggatgacgac accttttaat tttccatcct
     2941 ctacaactgc aagtggagtc ggtgagtcat aaataatatt gaaaatatca cttaccacca
     3001 tgtctttccc aatcaccgtc acattttcat ctaggaagtc gatcaatgga cgtttttgct
     3061 tcctagcttc taacgcttga tctgcagtga tgatcccttt caggtgtctc tttctgtcaa
     3121 cagccatcag catgctgact tcttcattac gcatccttgt caatgcaacg tttggtccat
     3181 cagattcgat attcgtcgtc aatgcaggta ccatgatgtt ttgagccgta agcactttgg
     3241 aacgatcgac ttcttccaca aattcacgta cataatcatt tgctggattg gtcaatattt
     3301 cttcacctgt tccgatctgc atgatttcgc cgtctttcat caaggcgatt ctgtccccaa
     3361 tccgtaaagc ttcattcaaa tcatgggtaa tgaaaataat cgttttttga acattggctt
     3421 gcaggtccag taattcgtct tgcatttctc gccggatcag cggatcaaga gccgagaacg
     3481 cttcgtccat cagcaagatc tcaggatcat ttgctaaagc tctggctagg ccaacccgtt
     3541 gttgcatccc accagataac tgactaggat attggtcttt aaatgaaagc aaactagaat
     3601 tttctaatgc tttttctgct ttttcttgtc gttcttcttt aggtacacca cggacttcta
     3661 acccgtattc tgtattttcc aagatcgttc gatgtggaaa cagaccgaag ttttggaaaa
     3721 ccatattgat tttatgacga cggacttctc gcaattcttc tttgcttaac ttcgatacat
     3781 cttctccatc aatatagata ctgcccgaag ttggctcaat cagacgattc aatagacgga
     3841 tcaatgtgga tttcccactc ccggaaagcc ccatgatcac gaagatttct ccttctttta
     3901 cgtcaaaatt cacgtcatac acaccaaccg ttgcaccggt tttctttaaa atctccgttt
     3961 tcggctggtg atcattcatc atatctaaag cggcttgtgt ctttttacca aatatctttg
     4021 ttaaatgttt tacttctact tttaccaaac aaattctcct tttttatgta aattcatttt
     4081 tctttctgaa aaaagtgacc acactatgtt aacatcatgt agtcactttg tcaaaaatga
     4141 aagaaatttg cttgtccaaa ctgaaaaaaa cttggctaaa tctttgtaaa taaaaggttt
     4201 agccaagtta atattttttt aatatttcac acaatcattc tgcaagctga tgctccgggt
     4261 aaaaaaagtt tgtcatacgc tgtagtgtat attcatttcc aataaaatga acgatgtccc
     4321 ctgccgtaaa tttagcatag gggccaggtg aaacaagcag ttcttcatta tgagaaattg
     4381 cgataactgt ggcgccagtc gcctgccaga tattcaactc gctaattgaa cggtccagat
     4441 gcattgcttt ttctgataaa gataattcaa acggaagtaa aggattcatc ttatgtattc
     4501 gcttcgtttg attgaccagc tgatccaata attgagaaaa atgattcagc tcatcttgtt
     4561 gcttctccac acttgccatg atttctgctt ttgtatcttg gatggattgt acatcttgat
     4621 attgcaatag gaaatctttt gctttttcct tcgaagcaac aaatgcccca cttccatgcc
     4681 gtacttccat gattccaaga tctaccagca cattgatagc cttgcgggta gtctcaggag
     4741 aagcattgta cgtactggca agtgttgagc gggcatggat cttctcaccc actttatatc
     4801 ggttttcaac gatacgttcg gctaaatcaa cagcaatctg ctggtagcgc gggcggacga
     4861 tttttttctt tccagacatt ttctttcctc tttctttttt tactttccct catcttacta
     4921 tgctctagca aaataagcaa aaaaggggag aatagcctct tgcaacgact gatcatcgct
     4981 tgatctgttc cttttttact gggcaagctg tttgacagtc atcacaattc ttaccggatt
     5041 ttaaacgagt atagacaatt gcgcctgcac ttccaaagat taacacactt aataagaatg
     5101 tcgccatcct attcctctcc ttctaatgac aatatagtat aaaactggtt cttggatttt
     5161 ggctgacgga acaaaacata aacgagtcct atcatcagta aaatagcaag atacgtccct
     5221 aacgctacct gtccaccctc caataagtgt cccaactgat aaatcacaaa actaactaaa
     5281 taagctaaac cgcattgata gcctatggcg atccaagtcc atttcttggc attcatttcc
     5341 cgatggatag ccccaattgc cgcaaaacaa ggcgcacaaa gtaaattgaa ggctaacaat
     5401 gaataagctg ctgcgggagt atatgtgtgt tgtagcactt gccagatttc tctgccattt
     5461 tcggatactt ccgcatgacc aaacaaaata ccaaatgtgc caataatatt ttctttagcg
     5521 accaatcctg taatcgctgc gactgttcct tgccagtttc cccaaccaag cggctgaaaa
     5581 acaactgcca ggattcgccc gaaaaaggct aagatacttt ggtcttcaga aacaggttga
     5641 gcataaaaat tataagaaga gctgaaccag atcacgatac tagaaacaaa gataatcgtt
     5701 cctgcttttt taacaaaaga cttgctgcgg tcataggttt gtcttaggac cgctgaccat
     5761 tgaggaaaat gatatgcggg caattccatg ataaaaggag ctgtatctcc agcaaacaaa
     5821 cgtgtttttt tcaagctgat tccagagaaa ataattgcag tcataccaag aaaataggca
     5881 gaaggagcta cccaagattg atgaggaaaa aaggcccctg ctactaaggc aataatcggc
     5941 aactttgcag aacaaggcat gaaggttgtg accatgatcg tcaaacgacg atcttgctcg
     6001 ttttcaatcg tgcgactggc catgacgcct ggcactccac atcctgtagc aatcagcata
     6061 ggaatgaagg attttcctga aagaccgaat ttcctgaaaa gtcgatccat gacaaaagcg
     6121 atcctcgcca tatatccgca gtcttccaga aagctcaagc ataaaaacaa tataatcaat
     6181 tgcggtagaa atcctaacac tgctcccacg cctgcgatga tgccgttcaa gatcagctcc
     6241 tgcatccatc ccgctacttg ccattcagct aacaatctgc caacatgttc tggtaaccat
     6301 gtaccaaaca atgtgtcatt gatccaatct gtccccattg ttcctactgt ttgaatagcc
     6361 agataataga tcagccacat cacaaatgcg aagatcggca aggctaacca acgattcgtt
     6421 gcaatctgat cgattttgtc actcatcgat aattggaaag aatcatttga cacaacacag
     6481 agtgtacata gttgggtaat aaattcataa cgttcattga ccagaatcgt ctcactatcg
     6541 tcgtccaata gtttctctgt taactggatc agttgctcga tctctttttg ctgagaaact
     6601 gctatctcgt aatcctctct taccttctga tctcgttcaa aatatttcat tgcataccag
     6661 cgtttcttgc cgctttgttc tcctagtaca tcttcaattt catgtaaagc tgcttctaaa
     6721 cgtggatcat atgtacaggg aacaggaata gaaggccgct tttttgcaga ctcctgaatc
     6781 ttttcgatta gtttttcgat tccttgcttt ttcaatacgc tgattggtaa aactggtaca
     6841 ccaagaccat aggaaagctt ttctagattg attttttttc cttctttttt gatgacatcc
     6901 atcatattca gaccaatgat cataggaatg tctaaatcca ataactgcgt agttaaataa
     6961 agatttcgtt ctaaattggt agcatcaata atgttgatga tcagttcagg ctgttcctga
     7021 accaaataat ctcttgcaat cacttcttct ggtgtataag gagacaaaga atagatgccg
     7081 ggtaggtcct gaatatgaat gttcggattt tttttcaacg atccttcttt tttttctact
     7141 gttacgcccg gccaattccc tacatattga ttcgaaccag tcaagagatt gaacaagcta
     7201 gttttcccgc tgtttggatt tccgactaac gcgatattca ttctttttcc tccctttgta
     7261 tcaagattag ttcggcctct gactttcgta acgtcagttc ataccctcgc actgtcagtt
     7321 cgattggatc tcccagaggg gctagttttc ggataactat ttttacattt cgcgtcaatc
     7381 ccatatccat taaccgtcgc ttcactgcgc cttgtccgtg gattgctacg acgatcccag
     7441 tctctcctgc ttttaattga tcaagagaag tccagctttc agaggaagca gtctttttat
     7501 tgacaaagat ttgacctagt atttcccgac ttactgccac tcgatgactt tttaccagta
     7561 aaatggcatt gttgcctgat aaattgacga cgacgatttt acttccggca acgatcccga
     7621 tattttgcaa atggcgtttt gttttttctt ctgtttcgat tcgttcgact tgataaacga
     7681 aattctgctt tgcctgcgct aaggtctgca tattgattcc tctcttgtga aataaatcat
     7741 gtattaaaat cattcattat atgttctaat tttcttacgt gttacgcaaa taaatgagaa
     7801 tcattctcac atagaatact ctcggtaatt gagaatgtca attatacttg tgatattatg
     7861 agcgaatctt tagtattgat agataaaaat gagaaaaaaa tatttcttca tagttttcca
     7921 caattttttc tctattaaat ttgctatttt cccacacgat acttgttttc agctcttttt
     7981 tcaaaaacac aaaacttagt attccacagt ttccacaaca caatatgtag tgtttgttgt
     8041 ttgacttttt tactaagagt tttatactat ctattataga taataagaat gtgttagagg
     8101 aggcactcac catgaacgta aaaatctttt ctaaaaataa ttgtatgcaa tgtaagatgg
     8161 tcaaacggtt tttaacagaa aatcaaattg cttttgaaga agtcaatatc gatgaacagc
     8221 ctgaagcagt agactggttg aaagcacaag gattccaaag tgttcctgtc attacttctg
     8281 atgcagaaac agttatcgga ttccgtcctg atcagttgcg taaacttgcc agctaacatg
     8341 actaaaagaa tcaaaacgtg atacatggat ttgaagggct tgagacagca ccagtttttg
     8401 tcgcaaactt ccaaatcctt ttttaaagct taaaaacaat gcaatgattt gcaatcaagc
     8461 atcatcaaat acgaaatgaa ggttgggcgt acgtatgagt cttaaaaatt tacaagatgt
     8521 cagttatttc aagctgaata atgaaatcaa tcggccagtc aatggtcaaa tccctttgaa
     8581 taaggataaa gaagctttag ttgccttttt tgaggaaaat gtcaaaccaa atacgatgac
     8641 ttttccatct attatggaaa aaatcaatta cttgatcgat caagaatata tagaagcaga
     8701 attcattcaa ttgtattcac catcatttat tgaaaagcta tatcaatttt tggacgagca
     8761 acatttttct ttcaaatctt ttatggctgc ttacaaattt tattctcaat atgcgttgaa
     8821 aaccaacgac ggttccgctt atctggaaac ttatgaagat cgagtagcct tcaatgcgct
     8881 ttactttgcg aacggggatg aagaattagc acttgctctt gcagacgaaa tgatccatca
     8941 acgatatcag ccagctacac cttctttctt gaatgctgga cgtaaacgtc ggggcgaatt
     9001 ggtgtcttgt ttcttgactc aagtcactga cgatatgaac tctatcgggc gctcaatcaa
     9061 ctctgctttg caattgtccc gtattggtgg cggcgtaggg attactttat caaatcttcg
     9121 tgaagccggc gctcctatca aaggatatga gggtgctgca agtggtgtcg ttccagtcat
     9181 gaaattattc gaagatagtt tcagctattc taatcaatta ggtcagcgtc aaggagctgg
     9241 cgtggtttat ttgaacgttt tccatccaga cattattgct ttcctttcag caaaaaaaga
     9301 aaatgcagat gaaaagattc gcgtaaaaac tctttctctt ggcgtgatcg tacctgataa
     9361 attttatgaa ttgacacgta aaaacgaaga tatgtattta tttagtccat atagtgtcga
     9421 aaaagaatat ggtgtcccat tctcttatgt aaatattaca gctgagtatg atcatctagt
     9481 tgcaaatcca aacattcgca aacaaaaaat caaagcgcgt gatcttgaaa atgaaatctc
     9541 aaaattacaa caagaatctg gctatcctta cgtaatcaat attgatacag cgaaccgcga
     9601 gaatccaatc gatggcaaga tcatcatgag taatttatgt tcagagattt tacaagttca
     9661 aacgccttct gtgattaacg gcaaacagga atatgaagta ttaggaacag atatctcatg
     9721 taacttaggc tcgacaaata tcgtgaattt gatgcaaagc cctaactttg gtcaatctgt
     9781 gcgtacgatg actcgtgcat tgacatttat tacagatgca tctgatatcg atgtcgtacc
     9841 accgatccaa aacggaaata agctgaatca tacgattgga ttaggtgcca tgggcttaca
     9901 cacttatctt gcaaaagaac aaatggaata tggctcagag gattcattag actttacaaa
     9961 tatttacttc atgctgttga attattggac acttgttgaa agcaacaata tcgctcgtga
    10021 aagaaaacaa gttttccata attttgaaaa atcagcttat gctgacggca cttatttcga
    10081 aaaatacgta actggagaat tccagccaaa atcagaaaaa gtcaaagcat tgtttgaagg
    10141 catttttatc ccaactgctg aagattggaa tgctttgaaa caagcagtaa tgaaagatgg
    10201 actgtatcat caaaatcgct tggcagtagc accaaatggc tcaatttcat atatcaacga
    10261 tacgagtgca agtattcatc ctatcactcg catgatcgaa gaacgccaag agaagaaaat
    10321 cggtaaaatt tattacccag cgccttatct atccaatgag acgattccgt attacacttc
    10381 tgcttatgat atggacatgc gtaaagtaat cgatgtttat gcaacggcac aacaacacgt
    10441 cgatcaaggc atgagtttga ctttattcat gcgttctgaa atcccagaag gcatttatga
    10501 atggaagaca acaacgaagc aaacgacacg tgatttgaat attttaagac attacgcttt
    10561 ccacaaagga atcaaatcca tttattacgt acgtaccttt actgatgatg cagaagaaat
    10621 cggaagcaat caatgtgaaa gctgcgtgat ctaatatcat agatagattc gcttattcat
    10681 attttaatta aaggagcatt ctcatgtcag aaacatatta tgaagcaata aactggaatg
    10741 aaattgaaga tatcattgat aaatccactt gggagaagct gacagagcaa ttttggctag
    10801 atacacgtat cccactttca aatgacctag atgattggcg tacattgtct caattggaaa
    10861 aagatacagt aggacatgta tttggcggtt tgacactgct agatactgtc caatctgaaa
    10921 gtggcatgga tcagttgcgt aaagatgtcc gcactcccca tgaagaagcc gttttgaaca
    10981 atattcaatt catggaatcc gttcatgcca aaagttattc ttctattttt agtacactga
    11041 atacgaaaaa agaaatcgaa gaaatttttg actggacaaa tacgaatcct tatctgcaaa
    11101 aaaaagctga gcgtatcaat gaaatctaca aacacggtac accgctagaa aagaaaattg
    11161 ccagcgtctt tttagaaaca ttcttgttct attcaggttt ttatactcct ctttattatt
    11221 taggaaacaa caaattggct aacgtagcag agatcatcaa actaatcatc cgagatgaat
    11281 ctgtacatgg aacctacatc ggttacaaat tccaattagg attcaacgag ttacctgaag
    11341 cagaacaaga aacgttaaaa gactggatgt acaatcttct ttatgagctt tacgaaaacg
    11401 aagaacgtta tacagaagaa ttatacgatc caatcggctg gacagaagaa gtcaagacat
    11461 tcctacgcta taatgccaac aaagcattga tgaacctagg gatggatcca ctctttccag
    11521 atactgccaa tgatgtcaac ccaatcgtaa tgaacgggat ttcaacaggc acaagcaacc
    11581 atgacttctt ctcacaagtc ggaaacggct atttgttagg tcatgtagaa gcgatgaaag
    11641 acgacgatta tttgattgga ttggaataag gatttatttt aattcaaaaa tttttcgaaa
    11701 aataaaaaga gtttagcgta cagatatttt aaatgtacac tgaactcttt tttatgctta
    11761 ttcacttgtt tcttccagca cttttggact gtctaacttt tccgaaacat gttccattct
    11821 tctcaataaa tagattttcc ggatatttaa tattatcgtc ttaatcactg catagacagg
    11881 tatgcaaatc agcatgccta atattcctgc gacacttcct gctccaatca ataccaagat
    11941 gatcgtcaaa ggatggatat tcattgattt cccaaataac aacggcttga tcaaatttcc
    12001 atctaattgt tggatcacta aaatcgacaa cgccatatac aaagcttgaa atggagagat
    12061 aaacaaaccg acaatgattg ctggtaccgc tccaataaaa gggccgacat acgggataat
    12121 attcgtgatt ccgcaaatga ccgcaagcaa caagctataa ggctgatcga atattctcat
    12181 tgccaaaaag ctcattacac caataatcaa agcatctaag atcgtactgc tgatataggc
    12241 agataatgtt tcgtttaatt cttttaccgt ttggcgtaac tctttacgaa tcgctttcgg
    12301 aaagaagtga gacaacgcat caataaattt gtgcccatcc ttgaacataa atagcaaaat
    12361 aaatggaacg gtaaataaca aaataaaaaa ttgcatcata aatgaaatga tttttgagac
    12421 acttgcagat aaactgacta atatgatatt gaacaaatta ctaagagata aatttgctga
    12481 tgctaattgt tcttccaaat tgaaattttt aaactcttcc cgctgcgcca attcatttaa
    12541 ccatttgctt gtttcatctg catacattgg caagctctgg gtcaattgaa ttgtctgttc
    12601 cattaactga ggcactacat tcatgacagt taaaacaact aaaatgatta cgaccaaaaa
    12661 cgtcaataaa aaaccgaaca ctctcgggat ccgatgcttt tcgagaaaaa caacgattgg
    12721 atcgaacata taatagataa aaccagcaac aagcaatggt aaaaaaattg ccgaaagcat
    12781 tttcaatatt ggactaaaaa tattcggcat ttgtaaaata aagtacacgc cgatgatcgt
    12841 gagtactagc tccactgtcc agaaaaataa ttttgattgt ttaaaacgtg attgcataca
    12901 tctcctccaa ggtcctttga tacttctatt ctatcattta acaaagagtt acgcgctgag
    12961 attttcattt gcttaaaaaa aacaaaaaaa ataaacatat tttaatgagt agacgagtac
    13021 ttaaacaaaa acacggtttg gagtagtgac aaaaattata ctgcagtccc tttttccaaa
    13081 taaacggtgt tcaaaagctt gcatcttcgt aataagccca gccaaacgaa aaatataagg
    13141 aactattttt atttggctgg gcttattact tgatgtagga catctatttt acaacctcag
    13201 atcatttatt gcgcaatact gactaaatgc aactttgtga tatttttgtg tacggacaac
    13261 tcctcgatca catcggcata agtgagccct tttggaagat caatcgtata gatatttttg
    13321 tacactcggt agtcatcaac gaattttacg tcaaaattca catcttcgat tacgatattt
    13381 ttctcttcaa aataatcggt aagaaaatct ttggtttctt ttctatggat aaattggatt
    13441 tctaactttt tagtcgtagg tacatggatc acgcgtttca ctactgtgag tgcaatagca
    13501 atcccgataa aactgatgat cgcaatggaa tagtaaccca tgccgatggc gatccctaat
    13561 ccagcaattg cccataaaga agcggcagtc gtaagtcccg tcaccgattg tttcgtgacg
    13621 atgatcgttc ccgcgcctaa gaaaccgaca ccactcacca cttgagcaat cagccttgct
    13681 tcatctgaac gaatcgttcc agcaagctgt ggatactcta aagcatctct taatgcacca
    13741 ctagcgatct caacttggat caatgcaatg atacatgctc ccatacaaac taaaatatgc
    13801 gtacgcatac ctgctggacg atttttatat tgtcgttcaa aaccgattgc tccgccaaat
    13861 agcatagcga tccctaaacg taaaataatc tctggaattg tcaataccat atcattcatc
    13921 taaacaccct ccagtccaat atatcatgtc tgaaaggagc agacaaaaaa agtgcaattt
    13981 ttacctctta cgaaaaaagg actgtgacaa aatttttgtc atagtccttt ttatgaataa
    14041 acggtgttca atcagctgac cctcggtatt actcaacaaa cggcaaaata tggggagcta
    14101 tttttccttt gtcttctcta atactcaggt caaaacgctg atttcacaac ctctttagcc
    14161 tactattgaa aattacaact tacttgctgc ttcttcatcg atgatcacaa caacattgtc
    14221 gtgattttgc aatacacttg ctggcaaatg attcgttaca ggtccttcaa tcatcccttt
    14281 gattgcatca gctttgcttt cgccaaatgc catcaagacg atttctttac ttttcataat
    14341 tgaaccgatt cccattgaaa cagcttttgt tggtacatct tctgctttat cgaagaatcg
    14401 tttgtttgca ttgattgttg attctgttaa gtcaactact gaagtcgttc cgtcaaatga
    14461 agcaccaggt tcattgaaac cgatatgtcc attttgcccg attcctaaga tttggatatc
    14521 gattggatgt gcatcgatga tttgatcata ctttttgcat gctgcttcca gatcttctgc
    14581 ttttccatct ggtacaaacg tttctttgaa tggtttttta tcaaataatt ggacatccat
    14641 gaaatgacgg tagctttgtt catctgaacc gcctagtcca acgtactcat ctaagtttac
    14701 agaagtcatt tcactgaaat ccaaatcgct tgaagtcatt tctttatata aagtaactgg
    14761 tgtgcttcct gttgccaatc ctaatacttt tgcacctttg ttcatacctt ctttgatgat
    14821 ttcaaatgct ttttttccgc cttcttcagc gttttttaca cgaatgattt ccatagtgat
    14881 tgctccttta tattttggta tagcccaatt atagcattct ttttttcttt ttacaataat
    14941 tagtatagac caaattgcat ttttttcatt ttcttgacaa taaataggaa tccgtgttag
    15001 gtttcataag atggttcaat caaacaatcc atgttgcatg aaaaccaagc aagtgattca
    15061 ctgtggacgc tttatgtctg cagttttttt atgaaaaagg agttattatg agtatactga
    15121 cattaaacaa cattacccag caatttggcg acaaaatcct ctatgaaggt gtcgatctgc
    15181 aattaaatgc tggcgaacat cttgggctga ttggacaaaa cggagccggt aaaagtacat
    15241 tgattaaaat catcactggt gagatcttac cagatgacgg acagatccat tggcaaaaaa
    15301 atattcacaa aggttacctc gatcaatatg tggaagtcga tgaaacatta acaatcgaag
    15361 aatttctaaa aactgcgtac gcaaaagaat acgaaaagga agaaaaaatt gcttttcttt
    15421 accaaacata tagcgagacc ttcgatgatt ccttgctgga aaaagctggg aaactccaaa
    15481 cggaattaga ccaaggagtg ttttatcaaa tcgacacctt agttgctgag atgagcagcg
    15541 ggttggggat cgatgtctta ggtttagatt cactgttgaa aaatctaagt ggtggacaac
    15601 gttcaaaggt gattttagca aaactacttt tagaaaaccc agatgttctg atcttagatg
    15661 aaccaaccaa tcacttagat gatcagcata tccaatggct gacgcagttt cttcaggatt
    15721 ttgacggtgc gtatatcgtg atttcccatg accaagaatt tcttgatcag atcaccaccc
    15781 atatcgcaga catcgagttt ggaaagatta ctaagtatac tggccatcta aaacaagcat
    15841 tgaaacaaaa ggaacaaaat cgcgaaagct atttgcgcca atatcatgct caacaaaaac
    15901 aaatcgaaaa gaccgaagct tatattcgaa aatacaaagc cggttcccga tcaacgatgg
    15961 caaaaagccg acaaaaacaa ttagataaaa tcgaacggct gacgcctcca gcttcagcaa
    16021 gtaaagctgt ctttgatttt ccattctcgc ctattgtcac gacattagca gtggaaacaa
    16081 ctgatttagt catcggatat gatcgaccgc tacttgcacc catcaatttg accatccgct
    16141 ttggtgaaaa tattgccatt cgaggcttca acgggattgg aaaatctact ttattaaaaa
    16201 cactgatcgg tgagattgaa aaaataagtg gcgatttcca cttcccagat aacacaaaaa
    16261 tcaattactt ttcacaagat ctgtactggg aaaatccatt agaaacacca ctacaatatt
    16321 taagcagtca atttccaaaa gcgactatca aagaattacg tcgccaacta gctaaagcag
    16381 ggttagttaa tcaattagca agtgaacctc tcactacatt aagtggtggg gaacaaacta
    16441 aggtgaaact tgcacagttg acaatgaacc aaggtaattt actgattctc gatgaaccga
    16501 cgaaccatat cgatcaagaa acgaaagaaa gcttacaaga cgggatacag aaatttccag
    16561 gaacagtaat catcgtctct catgaacaag aattttatca agatcttgtg caacgagtca
    16621 ttgagattga accaaaataa acgtttgatt gaatagttga aaaaaaggag ctgtgacaaa
    16681 agttttgtca caactccttc ttctgaataa atggtggcca attagctgac cttcggtatt
    16741 agctcaaaaa acggcaaaat atgaggagct atttttattt ggctattctt attacttgat
    16801 gcagaacagc ttttcacaac ctcttttcgt ctatttttta ttttcgagat ttctctttcc
    16861 tttaaaaaca actgtttgaa agaagaacgc acatccttga aaaacaagcc cgctaaccgt
    16921 tacacctaac cagccatact gctgccacat tgtcgtccct agcaatgaac cagccgcacc
    16981 tccaataaag tacataaaca taaataca
//
LOCUS       DANJMI010000056        16716 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000056 DANJMI010000000
VERSION     DANJMI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.056"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(253..1464)
                     /locus_tag="PWU81_002335"
     CDS             complement(253..1464)
                     /locus_tag="PWU81_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1111006.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKASSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLKHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFENLLKKDIK"
     gene            complement(1583..2218)
                     /locus_tag="PWU81_002336"
     CDS             complement(1583..2218)
                     /locus_tag="PWU81_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDL1111007.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAKNNVLQAQTGYFGTETVSIALHN"
     gene            complement(2354..3007)
                     /locus_tag="PWU81_002337"
     CDS             complement(2354..3007)
                     /locus_tag="PWU81_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111008.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQVTAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFFKYGIQ"
     gene            complement(3110..3316)
                     /locus_tag="PWU81_002338"
     CDS             complement(3110..3316)
                     /locus_tag="PWU81_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HDL1111009.1"
                     /translation="MPSYVVLQVVLKEKFIGKGSQNLSELENTINRQCSKGYRLHTIS
                     TTNGGSKGFGGGDRIQATLVFESL"
     gene            complement(3392..4138)
                     /locus_tag="PWU81_002339"
     CDS             complement(3392..4138)
                     /locus_tag="PWU81_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004564412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1111010.1"
                     /translation="MKQTTQQRLNQLMSERNLKQVDILNMSLPLQKETGIKMSKSHLS
                     QYVNGKSSPDQHKLYLLAKTLNVSEAWLLGYDVPKEDKKNNIPSIETIYNQLEKPRQT
                     KVYNFAEQQLEEQNRGNVVHFPKKEKLPTIKNSASAANPTELVYGDTVVEEEEFERVP
                     NNADFAVPIIGDSMEPVIRNGQFVFVKEQPDVEDGEIAIVEIDGDGVTCKEVYKDYES
                     QTIILRSINELYEDRVVSPEQIRIIGKVVF"
     gene            4298..4522
                     /locus_tag="PWU81_002340"
     CDS             4298..4522
                     /locus_tag="PWU81_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HDL1111011.1"
                     /translation="MSFDYSKLSGRIVEKYGTQYNFSIALGLSERSLSLKLNNRVPWK
                     STELQKAIELLDIPKEEIGEYFFNLEVQNV"
     gene            4546..5307
                     /locus_tag="PWU81_002341"
     CDS             4546..5307
                     /locus_tag="PWU81_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDL1111012.1"
                     /translation="MNTQKFENNLFELEVKTENGESLFNAETVARSLGFVETKNGKEY
                     VRWRTVNGYLKKYLSQEVAKNDFISEPMVYKLAFKANNVLAEKFQDWLATEVLPQIRK
                     HGMYATDELLNNPDLLIEVATKLKEERTLRLIAEQRVNELQPKADYYDRILNNKGLVT
                     VSTIAKNYGMSAVSFNKLLHELGIQFNQSGTWLLYSKFQDKGYTHIEPFDYEDKNGNR
                     QVKTRMKWTQKGHIFLYETLKKNNYLPMIEREQTA"
     gene            5319..5576
                     /locus_tag="PWU81_002342"
     CDS             5319..5576
                     /locus_tag="PWU81_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111013.1"
                     /translation="MNEEMSQTIKVQKMIDDLTHAIDSQADKIIKELQGQKVWDAKML
                     FKTIKFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            5668..5919
                     /locus_tag="PWU81_002343"
     CDS             5668..5919
                     /locus_tag="PWU81_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1111014.1"
                     /translation="MEQLALVNLSDLKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWVNSTEH"
     gene            5895..6068
                     /locus_tag="PWU81_002344"
     CDS             5895..6068
                     /locus_tag="PWU81_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111015.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKPTEGAD"
     gene            complement(6182..6589)
                     /locus_tag="PWU81_002345"
     CDS             complement(6182..6589)
                     /locus_tag="PWU81_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111016.1"
                     /translation="MSFDNTITISIILALVALISPSITAVINNKHVESMKDKEIELQK
                     HDSKTQTIQTTFSTFLNNVGICIGSNTDKNISAVKASGYAVLPYIQNEDIEVMKIFLS
                     RFGYGNTDAEQKSLETYLIDKVLPILNKSLEKL"
     gene            6750..6974
                     /locus_tag="PWU81_002346"
     CDS             6750..6974
                     /locus_tag="PWU81_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111017.1"
                     /translation="MISVKGLGDEIFEAMMSKAQQDIKEKIISAAAQGQTSCTVRSRG
                     TTPSFLLALEDEGILNIKRRDGSVRLFWQF"
     gene            6984..7178
                     /locus_tag="PWU81_002347"
     CDS             6984..7178
                     /locus_tag="PWU81_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111018.1"
                     /translation="MKDFDSLGARQQPPNEASPVGVDWQENPLYPGDTCYLTEEGYVP
                     VDAILEYVQQHYPKIELGGI"
     gene            7181..8203
                     /locus_tag="PWU81_002348"
     CDS             7181..8203
                     /locus_tag="PWU81_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HDL1111019.1"
                     /translation="MANDLTQTTQRSLDEQVISNLGRLQEQGLEMPPGYSPQNALKSA
                     FFELTNNTGGNLLQMAANNQEMKTSISNALLDMVIQGLSPAKKQCYFIKYGNKVQLMR
                     SYFGTMAVLDRVTGGADITPVVVRQGDEFEVAMDGPNMVVKKHETKFENLDNEIIAAY
                     VVIKLTNGKETTTVMTKKQIDQSWAKSKMKDSGPQKEFPEEMAKRTVINRAAKTLINT
                     SNDNDLLVQAAKDTLENEFDNDRKDITPQTEKVATLEQKFFSNKKITEPIQKEPDPIV
                     IPDDIQNEVTRVADVPAHPEIEQAHLIENEDINPIQEELIDIPDFGREEGVEDVSEFE
                     DDEYPF"
     gene            8166..8981
                     /locus_tag="PWU81_002349"
     CDS             8166..8981
                     /locus_tag="PWU81_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HDL1111020.1"
                     /translation="MSQNLKTTSTLSDENYYSNEADWQYMSTSQYKSFLKCEAAALAK
                     LKGDWAPTSDPKALLVGNYVHSYFESKEVHEAFKEENKSKMFSSRKPYGLLKDFQIAE
                     QMIERLKQEEAFMNIYQGEKETIVTGELFGTTWKGKIDCLNVEDEYFVDIKTTKDMHE
                     RKWNENYGSRETFIVNFGYVLQMAIYQELLLQQYGKMFMPIIAAVSKQTPSEAKLITI
                     DQDKMSYELIMLKENIERIVKVKNGEERPIPCGTCEYCRGNLSITGFTSMDDL"
     gene            8999..9781
                     /locus_tag="PWU81_002350"
     CDS             8999..9781
                     /locus_tag="PWU81_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111021.1"
                     /translation="MNLGYIKLYRKVTSSFVWTNSDMFKLWILCLMKASHEDRKFLFN
                     GQEVRLTSGQFVTGAHAIAKEYNEGVPSDKAIAWRTLWRWLKKFENEELLTIQSNARY
                     SVITIINWSDYQSSDKPVTSDGQSSDKPVTTNKNDKNDKNDKNNNPRNSRKTREYADD
                     DPNKKLAILLLKLIRKNRSLKEPDLNKWANTIRLTIEADKRTGKEVQDMIVWATNHDF
                     WSTVISSPTSLRKNFDTMVGQKNKRKQQNISNDELPETGEDW"
     gene            9781..10629
                     /locus_tag="PWU81_002351"
     CDS             9781..10629
                     /locus_tag="PWU81_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL1111022.1"
                     /translation="MNKKLSAMAAPYGGLKTADHNCPKCGDPLYIWKTKNKDGTDRCG
                     PTCINKICGYREMVTKNQKEAIQKANEAMKKDAINRMLNSSMITDDAIWTFDFDGYKV
                     VDQETAQIKAMAQEWAKKIVNGSTIHAVITGRTGAGKTHLGAAVIKEVMRASNYKIAC
                     SFISYRELLEQLKFAMNDPEARKAVTGSLMAEIKKSDFVVIDDLGAELGRMEENNQAT
                     PYDVDVLTSLTEARLNKATIFTTNLSSKQLKHAYGERVFSRVMNGTKGNIAVFKTTTD
                     KRRNPV"
     gene            10626..10868
                     /locus_tag="PWU81_002352"
     CDS             10626..10868
                     /locus_tag="PWU81_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111023.1"
                     /translation="MTFVVKKMCYLDSRGRGEASVEFAKHHTTKEEADLVASVCGGEV
                     VEVIKPKRRFSKPKTKPVKKECCCPKSNQAWMRGAK"
     gene            11011..11379
                     /locus_tag="PWU81_002353"
     CDS             11011..11379
                     /locus_tag="PWU81_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDL1111024.1"
                     /translation="MATRSKYGNKKHEVDGITFDSITEAEYYDLALWQAQANGWKVKL
                     QERFELMPKFELEGKKYRKIEYIPDFTFYKSGKLVKVVDVKGMQTKDFKIKAKLFCHQ
                     YQVPLILAKKYRNTFKEERF"
     gene            11394..11555
                     /locus_tag="PWU81_002354"
     CDS             11394..11555
                     /locus_tag="PWU81_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL1111025.1"
                     /translation="MTTEEVIQMRIRSLRREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            11552..11878
                     /locus_tag="PWU81_002355"
     CDS             11552..11878
                     /locus_tag="PWU81_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111026.1"
                     /translation="MSVDKEAAWRKLMLLIQDENWQEDEAVVAEVQRLGKIANGRIRK
                     KPDKRKQRKGKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYK
                     YLEEEK"
     gene            11875..12690
                     /locus_tag="PWU81_002356"
     CDS             11875..12690
                     /locus_tag="PWU81_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HDL1111027.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
     gene            12829..13140
                     /locus_tag="PWU81_002357"
     CDS             12829..13140
                     /locus_tag="PWU81_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL1111028.1"
                     /translation="MDELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGTNVQECLEMAYNEIKGRTGKMVDGVFVKSSDLEGS
                     K"
     gene            13164..13382
                     /locus_tag="PWU81_002358"
     CDS             13164..13382
                     /locus_tag="PWU81_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111029.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            13379..13795
                     /locus_tag="PWU81_002359"
     CDS             13379..13795
                     /locus_tag="PWU81_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HDL1111030.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIYENSELLEVE"
     gene            13792..14130
                     /locus_tag="PWU81_002360"
     CDS             13792..14130
                     /locus_tag="PWU81_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111031.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMYGNSESAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            14127..14357
                     /locus_tag="PWU81_002361"
     CDS             14127..14357
                     /locus_tag="PWU81_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111032.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            14364..14567
                     /locus_tag="PWU81_002362"
     CDS             14364..14567
                     /locus_tag="PWU81_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HDL1111033.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            14564..14860
                     /locus_tag="PWU81_002363"
     CDS             14564..14860
                     /locus_tag="PWU81_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111034.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            14937..15350
                     /locus_tag="PWU81_002364"
     CDS             14937..15350
                     /locus_tag="PWU81_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL1111035.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            15363..15749
                     /locus_tag="PWU81_002365"
     CDS             15363..15749
                     /locus_tag="PWU81_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111036.1"
                     /translation="MWKDYVSLKELKKDLVFKRIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHTSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKDKLCIVTDA"
     gene            16300..16527
                     /locus_tag="PWU81_002366"
     CDS             16300..16527
                     /locus_tag="PWU81_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111037.1"
                     /translation="MRTYWYVSLNNKYPKPMKGQHRRVVMSVQMKAKYSIVEMLREAT
                     PKEIDQYKLVLIGIGWFKDEHIQTNMKRWLR"
     gene            16524..>16716
                     /locus_tag="PWU81_002367"
     CDS             16524..>16716
                     /locus_tag="PWU81_002367"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111038.1"
                     /translation="MKLYYVEKANCWVVEGCLSKYATDIISTQFSELGLKVIFCGEFV
                     SVKEAVLPMTAKSRRSRGSS"
BASE COUNT         5666 a         2726 c         3485 g         4839 t
ORIGIN      
        1 aataaaaaaa gaacagcgta aatgttttcg aaatgctttc gcaccaacat ttacctgttc
       61 cgtgggtttg gcacccaatg ggccgtgagg ggctcgaacc cgcgacccgc tgattaagag
      121 ttcgttattt aatggtattt taaatgaaga taagcgaatt tacagcattt ttaaatgctt
      181 atataccgca aattatccca agtttttgta tatcctaccc gagtttatac ccgaaaatat
      241 aattttttag tgttatttta tatctttttt taataggttt tcaaacgctg tagttgctgt
      301 atctttttct ttgcgtaaga catggccata aacattcatt gtagtattaa aatctcggtg
      361 tcccaatcgt ttttgaagct cttttggagt catattagga tcgctaagca aataggtagc
      421 tgatgtatgt ctaatgtcgt gaaaacgaat gtgacgcaaa ttgtgacgtt tggtgaatcg
      481 tttgaacttc ttatacagat aacttccacg tggcaaagtt ccatcttgtt ttgtatcaaa
      541 tataaaatag tgttttaatt tctttatatt taatttccaa cgaatctttt tattctcttt
      601 gattaattca tctaacatat caaatagata agcaggaccg gttactattt tttcatcatc
      661 atcatttttc aaacctggca gcaaccgaat atctgatttt ccatccactt ctgaaatccg
      721 ctgatgaaat cttatttctt gtttgtcaaa gtcaatatct ttttcttcta aagctgcaat
      781 ttctccttgt cttgctcctg taataaatgc tagaacaatt aaagttttaa tttctatgct
      841 ttcctcaaat gctgctttta gcattgcatg aatttcttct tccgaataag gttcttcgat
      901 attctttttt acgccttttt cttttggtat ttttacattt tccacaggat tttctttaat
      961 tgctccgtac tcatttttag ccatcaaaaa taagttgtta acggcgtata acatacgaag
     1021 ctttgtattt cttgataaag gtttatcaga gtttctccca ggatcttttg tattgattct
     1081 ttgagcatta acaacaattt tctttatcat gtatggtttg atatcgatta gagggataaa
     1141 atcaaattta tcaagaaatc tattctcgat cattcgctta tagttatgat aagactttgg
     1201 ctctaaattt ttttcggcct ctgaaagcca tatttttcta taaaattcac caaaagttat
     1261 attgaacaaa tccaacgaat catcggtcat tccttcgaat tgctctaacc agatgttcaa
     1321 ttctttatat gccgctcttt tactacttgc ttttatagtt ttgctttttc tttttgccga
     1381 gccatcaggt ttgtatccta atacagctct tagtctatat gtttcgttgt ctacttgttc
     1441 taagtatcct gttacttctg acataattga atcagctctc tttctctgat acaataggta
     1501 ctataaagaa gcctatcgta taggtttgtt tctttcttag aacacgctcg ctttggtcgg
     1561 tggggcgtgt tttttatact aattagttgt gaagagcaat tgaaactgtc tctgttccaa
     1621 agtaacctgt ttgtgcttgc aatacattat tttttgcatt tgcttgggct tcagagatat
     1681 caaaaactat tttaccagtt tgttgcatat caggatttaa gctttccata aagaaagagt
     1741 tagtgatatt accattttca tcttgatttg ccgacataga tgcagccgaa tcagcttcga
     1801 aagtttttcc atctgctttt aatttgaaga atgagctatc aacagtcaca gccttatcac
     1861 ctgcattttt tacagataag tcaactacta agaaagtgtc ttttgcgtta gtaggaagta
     1921 cagatgggcc aacttgtttt actacttcaa cggagtttac tttgtattcc attttcccta
     1981 cagcaacgtc atcaccgatt ttataagtct tttcttcttt tgttgtagtt tcttttgtag
     2041 tttctttatt agtacttgtt gatgttgcag tagctgaatc tttgttttca ccgccactta
     2101 gagcaccacc aataccaaaa aacaaaagta ctattaatac ccaaaaccat acccgtttat
     2161 aaaacggttt tttaacctta tacattttcc catcttgacc cataactttt tttgccattt
     2221 aaatattcct catttcttgt tataatatat ttgtgatctc agaaatgagg tatgagtccg
     2281 tgttgcagca cgggcttttt tatttataac ttttttagag attataggca aaatagtagg
     2341 gcataaaaat atattattga attccgtatt taaaaaatcc taattgaact tcaccggaag
     2401 tcttttgctg tgttgtacgc aatgcttctt cttcagacat tccattctgt actttccatg
     2461 caacaggcga catcccgtat ttgttaacaa aatcagtaag tgataaagtg tcagcgtctt
     2521 gctgagcgct tgtttgttgg tcttctggat tttgttgaga tgctgcttgt tgttctttct
     2581 gatcttgact gataatattg ccagcatcat ctgtagtcaa tccattttca taaagggcca
     2641 cgccgaaagc ttcccactct ttgttggacc aatttgcacg atcagctgga gttgactgta
     2701 aagtgcgttg tttcatctgt tcatatgttt cttcttgagg tgcggtttgg attgtactct
     2761 gactggagct tataactgtt gggctaggtt ccgccgtaac ttggttggag cttgtaactg
     2821 ttgaactaga atctgtttta gatgtagatt tgctagtaga tgaactggtt tcagttgttt
     2881 cttttgtttt acttattttt gtttcttggt tagaagtggc atctgttgat tcagcttttt
     2941 tattatttga acaagctgaa agtagcagag cagtacttaa caacaacata acgctaactt
     3001 ttttcatttt atactcctct tttctttgat acaataagtt gtaaagaagc ctactatagg
     3061 tttaacatcc cacgtccaca aactttggtc ggtgggcgtg tttttttgtt tatagacttt
     3121 cgaaaactaa agtagcttgg attctatcgc caccaccgaa acctttacta ccaccgtttg
     3181 tagtggaaat agtgtgtaat cgataacctt tagaacactg tctatttata gtgttttcaa
     3241 gttctgatag gttttgcgac ccttttccta taaacttttc ttttaataca acttgcaata
     3301 caacgtaact tggcatattc ttttccttct ttctactatg ataataaata ctaaccccta
     3361 gccggagcgc caactacgac tggggtattt tttagaatac gactttcccg attattctaa
     3421 tctgttccgg tgagacgact ctatcttcat ataattcgtt tatagagcgt agaataatcg
     3481 tttgactctc ataatcctta tatacttctt tacacgtcac accgtcacca tctatttcaa
     3541 cgatagcaat ctcaccgtct tccacgtctg gctgttcctt aacaaataca aattgtccat
     3601 tcctgataac tggttccatt gaatcgccta taataggaac agcaaaatca gcattgtttg
     3661 gaactctttc aaattcttct tcctctacaa ctgtatcgcc ataaactaat tcagtcgggt
     3721 tggcggcaga agctgaattt ttgattgtcg gaagtttttc ctttttagga aagtgcacca
     3781 cgtttcctcg attttgctct tcgagttgct gttcagcaaa gttgtagact tttgtttgtc
     3841 ttggtttttc taattgattg tagattgttt caatggatgg aatgttattt ttcttatctt
     3901 ctttaggaac atcgtaacca agcaaccatg cctcactcac atttaaagtt ttagccaaca
     3961 aataaagctt gtgttggtcg ggcgatgact taccattgac atattgcgat agatgacttt
     4021 ttgacatttt tattccagtt tctttttgca aaggtaaaga catattcaaa atatcaactt
     4081 gctttaaatt tctttcagac attaactgat taagtcgttg ctgagtcgtt tgcttcatct
     4141 tatacctcct ttctgatact tattatatac cttattgaac ataagttcaa taaaaaagtt
     4201 aaattaattt gaacttttat gttgacaatg ttttatgcag gagttatact ttggttacaa
     4261 agttcaaaac atttgaacta gaaaggagcg aactaatatg agttttgatt attctaagtt
     4321 gtcgggaaga attgttgaga aatatggaac gcaatacaat ttttcaatcg ctttaggttt
     4381 gtcggaaaga agtttgtcat taaagttgaa taaccgagta ccatggaaaa gcaccgaact
     4441 tcaaaaagcc attgaattgc tagatattcc aaaagaagaa attggcgaat atttttttaa
     4501 tttagaagtt caaaatgtct gaacttaaga aaggaattcg aaaaaatgaa cacacaaaaa
     4561 tttgaaaaca atctatttga attagaagtt aagacggaaa atggtgaatc gttatttaat
     4621 gctgaaacag tagcaagaag tttgggtttc gtggaaacaa aaaacggtaa agaatatgtt
     4681 cggtggagaa ctgtcaatgg atatttaaaa aaataccttt cgcaagaagt tgcgaaaaac
     4741 gactttatca gtgagccaat ggtttacaaa ttagccttta aagcaaacaa cgtacttgct
     4801 gaaaaattcc aagactggtt agcaacggaa gtcctacctc aaatccgaaa acacggcatg
     4861 tacgcaacag atgaactact taacaatcca gacttgctga ttgaagtagc aaccaaactc
     4921 aaagaagaac gcacgttacg attgatagct gaacaacgag taaatgaatt acaaccgaag
     4981 gcagactatt acgaccgaat cttgaacaac aaaggattag ttacagttag tacgattgct
     5041 aagaactacg gtatgagcgc tgtatcgttt aataaattgc tacatgaatt aggtatccag
     5101 ttcaatcaaa gtggcacatg gctgctatac agtaagttcc aagacaaagg atacacgcat
     5161 attgagccat ttgattatga agataaaaac ggcaatcggc aagtaaaaac aaggatgaaa
     5221 tggacgcaaa aaggacacat cttcttatat gaaacattga agaagaataa ttacttaccg
     5281 atgattgaac gtgaacaaac agcataggag gtaacactat gaacgaggaa atgagccaaa
     5341 caatcaaggt acaaaaaatg atagatgatc taacacatgc aattgatagc caagccgata
     5401 aaatcatcaa ggaattgcaa ggacaaaaag tttgggatgc aaaaatgctt ttcaagacta
     5461 tcaaatttga aatgaatcca acaaaaagaa agctcgccga tgtgttggaa gaaaagttgg
     5521 cttccgctat caacgagcaa gaattactat ttgaaaccga tacttttaac agctgatgtt
     5581 ttatagatga cagtttcatc cgaatctata tcattataga ttcgattatt gcaatgtacg
     5641 aattataaat acaggaggaa ataaccaatg gagcaactag ctttagttaa cctctcagac
     5701 cttaaagctt ctttggcaga atctgagatt gcaaatgaag tgtgggatac aaagcaagct
     5761 gcggaatatt taaaaaccac aacacgcacg ctgactaaag atgccgaatc tggaaagatt
     5821 ccagcagcga aagttggcag agagtggagg tttagtagta tcgctttgta tcagtatcta
     5881 aaaggaggaa aaacatgggt aaattcaaca gagcactagt gttcagcgca cccctaatcg
     5941 tctatgcttt aggactttgg ggaagcagac aagcattaat cggcacgatc gtttacatgg
     6001 tttggatttt tatggggctt gatgaagctg agtacagagc gaaaaagccc accgaggggg
     6061 ctgactaaag tgtggttctg tttattaggt gtttatctcg tggccgtttt agtaaataac
     6121 tacatgagaa aacgtggcga atactggtat acatcctatg cggttctagt atgtctaatg
     6181 cttacaattt ttctaatgat ttattcaaaa taggtaatac tttgtcaatc aaataagttt
     6241 caagagattt ttgttctgca tcagtgttgc cgtaaccaaa acggcttaag aaaattttca
     6301 taacctctat atcttcattt tgaatatatg gcaggactgc ataacctgat gcttttacag
     6361 cagatatatt tttatcagtg ttactaccaa tacaaatacc cacattgttg agaaatgttg
     6421 agaaagttgt ttgtattgtt tgggtttttg aatcgtgttt ttgtaattca atttctttat
     6481 ccttcatcga ttcaacatgc ttattattta taaccgctgt aatcgaaggg gatataagag
     6541 ctactaaagc taggataatc gaaattgtga tcgtgttatc gaaactcatt ttttcaccat
     6601 ccagttttta aaccattata tcaaaaagga gagaagaaat aatgcaagaa ttagtaattt
     6661 tgaaaaataa aaaaagacac gaacgccagc aagcatgatc gtgtccaaaa gaaaaatctt
     6721 ttaaaccatt ataacacagg aggaaataaa tgatctcagt taaagggtta ggcgatgaaa
     6781 tattcgaagc aatgatgagc aaagcacaac aggacatcaa agaaaaaatc atttctgctg
     6841 cagcgcaagg acaaactagc tgcactgttc gatcgagagg aacgacacca agttttcttt
     6901 tggcgctaga agatgaaggc attttgaata tcaaacgaag agatggcagc gtcagattgt
     6961 tttggcaatt ttaggaggcc gtgatgaaag attttgattc tttaggcgcc agacagcaac
     7021 caccaaatga agctagtcct gttggagttg attggcaaga aaatccgtta tatccaggtg
     7081 atacttgcta tctaacagaa gaaggctatg taccagtaga cgcaattcta gagtacgtcc
     7141 agcaacatta tccaaagatt gaattaggag gaatttagga atggcaaatg atttaacaca
     7201 aacaacacaa cgttcattag atgagcaggt aatcagtaat cttggacggt tacaagaaca
     7261 aggtttagaa atgccaccag gatatagtcc acaaaatgcg ttaaaaagtg cttttttcga
     7321 actcactaac aatacaggag gaaacttgtt gcagatggct gcaaacaatc aagaaatgaa
     7381 aacctccatt tctaatgcac ttttagacat ggttattcaa gggttatctc cagcaaaaaa
     7441 acaatgctat ttcattaaat acggaaacaa ggttcagcta atgcgttcat attttggaac
     7501 aatggcggtc cttgatcgtg taactggagg ggcagatatt acgcctgtag tcgttcgaca
     7561 aggtgatgag tttgaagttg caatggatgg accaaacatg gttgtcaaaa agcatgaaac
     7621 aaaattcgag aatttagaca atgaaatcat tgcagcctat gtagtcatca agttaacaaa
     7681 tggtaaagag accacaacag tcatgacgaa aaagcaaatc gatcaaagtt gggctaagtc
     7741 aaaaatgaaa gattctggac cgcaaaagga atttccagaa gaaatggcta aacggacagt
     7801 aatcaatcgg gcagctaaaa cattgatcaa cacaagcaac gacaacgatt tattagttca
     7861 agctgcaaaa gatacgttag aaaatgaatt tgacaatgat cggaaagata taacgccaca
     7921 aacagaaaaa gtggctactc tcgaacaaaa attcttttca aacaaaaaga ttactgaacc
     7981 aatacaaaaa gaaccagatc cgattgtgat tccagatgat atccaaaatg aagtaactcg
     8041 agttgctgat gtacctgccc atccagaaat agagcaagca catttgatcg aaaatgagga
     8101 tatcaatcca attcaagaag aattgataga tattccagac tttggacgcg aggaaggtgt
     8161 agaagatgtc tcagaatttg aagacgacga gtaccctttc tgatgagaat tattactcca
     8221 atgaagctga ctggcagtac atgtcaacat cacaatataa gtccttttta aaatgtgaag
     8281 ccgcagcctt agcaaagttg aaaggcgatt gggcaccgac gtcggatccc aaagccttac
     8341 tcgtaggaaa ctacgtacat tcttattttg aatcaaaaga agtacatgaa gcgttcaaag
     8401 aagaaaataa atccaaaatg ttttctagta ggaagccgta tggattgttg aaagatttcc
     8461 aaattgctga acaaatgatt gaacgactta agcaagaaga agcattcatg aacatttatc
     8521 aaggtgagaa agaaacgatc gtgactgggg aactatttgg aacgacatgg aaaggcaaga
     8581 ttgattgctt gaatgttgag gatgagtatt ttgttgatat caaaacaacc aaagacatgc
     8641 atgagcggaa atggaacgaa aactacggat caagagaaac gttcattgtc aattttggtt
     8701 acgtacttca aatggcaatc tatcaagagc ttcttttaca gcaatacgga aagatgttca
     8761 tgccaatcat cgctgcggta tcgaagcaaa cgcctagtga ggcgaagttg ataacgattg
     8821 atcaagataa gatgagctac gagctaatca tgctaaaaga aaatattgaa agaattgtca
     8881 aagtgaaaaa tggcgaagaa aggccgatac cttgcggtac gtgcgagtat tgtcgaggta
     8941 acctttcaat tactggattc actagcatgg acgatcttta aaaggaggtg ctttgcgaat
     9001 gaatctagga tacatcaagt tataccgtaa agtgactagt tcattcgttt ggaccaattc
     9061 cgatatgttc aagctatgga tattgtgttt aatgaaggct agtcatgaag atagaaaatt
     9121 tttatttaac ggtcaagaag tacgtttgac aagcggacaa ttcgtcacag gagcccatgc
     9181 aatagcaaaa gagtacaacg aaggagtccc gagtgacaaa gcaattgcat ggcgaacgct
     9241 atggagatgg cttaagaaat ttgaaaatga agaattattg acaatccaat cgaacgctag
     9301 atatagcgtt ataacaataa taaattggtc tgactatcaa tcgagtgaca agccagtgac
     9361 aagcgacgga caatcgagtg acaagccagt gacaacaaac aagaatgata agaatgataa
     9421 gaatgataag aataataatc ctcgcaactc tcgaaaaaca cgagagtatg cagatgacga
     9481 tccaaataaa aaattggcca ttcttttatt aaaactcatt cgaaagaatc gtagtctcaa
     9541 agaacccgat ttgaacaaat gggccaatac gattcgtttg acgattgaag ctgacaaacg
     9601 aactggaaaa gaagttcaag acatgattgt ttgggctact aaccatgatt tttggtccac
     9661 agtcatttca tcgccaacta gcttaagaaa aaatttcgat acaatggtgg gtcaaaaaaa
     9721 taaaagaaag caacaaaata tttctaatga tgagttacca gaaacaggtg aggattggta
     9781 atgaataaaa aactaagtgc aatggctgca ccttacggcg gattaaaaac agcagatcat
     9841 aattgcccga aatgtggtga tccattgtat atctggaaaa caaaaaataa agatggtact
     9901 gatcgatgcg gtcctacatg tatcaataag atttgtggtt atcgagaaat ggtaactaaa
     9961 aatcaaaaag aagctatcca aaaagcgaat gaagcaatga aaaaggacgc tatcaatcga
    10021 atgcttaaca gttcaatgat tacagatgat gccatatgga ccttcgattt tgatggatac
    10081 aaagtagttg atcaggaaac agcacaaata aaagcgatgg ctcaagaatg ggctaaaaaa
    10141 attgtaaatg gtagcacgat tcatgcagtg ataaccggta gaacaggagc cggcaaaacc
    10201 catctaggcg ctgcagtgat taaagaagtc atgagggcat ctaattataa aattgcttgt
    10261 tcatttatca gttatcgaga acttttagaa cagttgaaat ttgcaatgaa tgatccagaa
    10321 gcaagaaaag ccgtaacagg ttcgctgatg gctgagataa agaaatcaga tttcgtggta
    10381 atcgacgatt tgggtgcaga actaggaaga atggaagaaa acaaccaggc aacgccttat
    10441 gatgttgatg ttctcacatc gctcacagaa gctcgtctaa acaaagctac gatattcaca
    10501 accaatttat catcgaagca attaaaacac gcgtacggtg agcgagtgtt ctctcgtgta
    10561 atgaatggaa cgaaagggaa catagctgta ttcaaaacaa cgacagacaa aaggaggaat
    10621 ccagtttgac ctttgtagtg aaaaaaatgt gctacttaga tagccgaggg cgaggagaag
    10681 ctagtgtcga gtttgctaaa caccacacga ctaaagaaga ggctgacctt gttgcaagtg
    10741 tttgtggcgg cgaagtagtc gaagttatta aacccaaaag acgattttca aaacctaaga
    10801 caaagccagt gaaaaaagag tgttgttgtc cgaaaagtaa ccaagcatgg atgagaggtg
    10861 caaaatgact tgtttaaaat gcaacgatga aacagttatt tggtataaga cattgcttgg
    10921 atggtcaact tgtgaacctt gtccaatatg caatgaaaat ggacgacgtt cgaaagaacg
    10981 actcgaaaga ctaaagaagg agtatagcaa atggcaacaa gaagcaaata cggaaacaaa
    11041 aagcacgaag tagacggcat cacttttgat tctatcacgg aagcagagta ttacgatcta
    11101 gccttgtggc aagcacaggc gaatggttgg aaagtgaagc tccaggaaag gtttgagctg
    11161 atgccgaagt ttgaactaga gggcaagaag tatcgcaaga tcgagtatat tcccgacttc
    11221 acattttaca aaagcggcaa gctggtcaaa gtcgtagatg ttaaggggat gcagacaaaa
    11281 gactttaaga tcaaggcaaa gttgttctgt catcaatatc aagtgccgtt gatattagcc
    11341 aaaaaatatc ggaatacgtt caaggaagag cgtttttaac gaggtggtcc atcatgacaa
    11401 cagaagaagt gattcaaatg cgaattcgaa gccttcggcg tgagattgac gatctggaac
    11461 gaacaaaggc agtgatggtc aatgaaacgg ctagaaaggc aatcgatttg cacatagaga
    11521 atttaagaag ggaaatccgt agattggagg aatgagcgtg gataaggaag cggcttggcg
    11581 aaaattaatg ttgctgattc aagatgagaa ctggcaagaa gatgaagcag tggttgctga
    11641 agttcagcgt ctaggaaaga ttgctaacgg acgtatacga aaaaagccag acaaaagaaa
    11701 acagcgcaaa gggaaaatcg tcgttgtttt acacgaaggc aaaattttga tgcaaggaac
    11761 agctagtgag ctgtctgcag aaactggata tacgcgtggg actattcgaa cgtacgcttg
    11821 gcgaaatcac accgatcgaa aagggcacga atataagtat ttggaggaag aaaaatgaac
    11881 gaaaacaaat taatcaaatt gggtgtagca ggagcagtaa tcgtaggtat tggagttatc
    11941 ggaggattta agttcttcga aaaaatcgat aatggatatg tgggtgtgcg ctattcaatg
    12001 aacggcggta tcaaagatga agcactgacg caaggtgtga aatttgtagg gattgacaaa
    12061 gtgatccaat atccaattcg cttgcaaact atccaatcaa aaaatatttc agtatctaca
    12121 agcgacggca aaaagacaac gattgatatc aaatatgact acaaagttga ttcaactaaa
    12181 gcagcaaaaa tgtacaagga atttgggaat atcacttcgg aagatatcga aagtggatgg
    12241 ttaaaatcta agcttcaaaa ggtcgctcgt gaagtttatg cgaaatatag tctgcttgat
    12301 gtcctttcag gagattcctc taaagttgaa gctgaggtat taacgaactt tgctaaatca
    12361 gttgaatcta aagggtttga agtcgaagac gtaacacttg gtgttccaga tgtcgataaa
    12421 gaaacacaaa aatcaatcga tgcgatcatt cgagctggtc aagaaaatga aaaagcgaag
    12481 ctagacgcag aaactgcaaa aactcaagct gatagtgaag cttacaagaa aacaaaagct
    12541 gcagaagcag aggcagaatc taatcgcaaa gtcgccgaat cagtaacaga caatttgatt
    12601 cgttatgaag aagctcaagc tcgcaaaaag catggatggg taacagtaaa tggagcagat
    12661 actgtagtta cggatgaagc aggcaaataa tatgggattc tttatggcta aaattctctt
    12721 gttcttaggt ttagtaggag cagcatatct cgtgtatgcg ctcctttccc aaactgatga
    12781 caaagaagat gacaacaacg atgaaatgaa attttaggag gagaaataat ggacgaacta
    12841 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcaga tccaaaagca
    12901 cagtttttga aagtagctga ggaattcggg gaaatagctt cggcgatggc aagaagtaat
    12961 gatgaactat ttaaagatag cgtaggagac gttatcgtca cgctgattat cctttccatg
    13021 caaaaaggga caaacgtaca agagtgttta gaaatggcat acaacgaaat caaaggacgc
    13081 acagggaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggaggg cagcaaatga
    13141 taaaaaagct cattcaattc agcatggatt tatatgatat cgaatcagga gcaacactat
    13201 ctgtggaatc ggaccatcta atcataaatt ttggtggaaa gcgccagatt attttgtggg
    13261 tagttgatga tgtactgttt ccagaaattg ttcatgattt cgaagaatca aaagcggttg
    13321 agtttgaaat agtgaaaaaa gtaatggaat tgattgaaaa atacgaggag gacagcgaat
    13381 gataccgaag tttagagcgt gggataaacg aaagaacgta atgagagatg tagccgtctt
    13441 gcattttact aaaaacggca aaacaaactt tattgaatat tggataaatc ctaccgaatt
    13501 gaaatcatat cacgtgcgaa acatcgacct catgcaatcc acaggaatga aagataagaa
    13561 tggtgtggat atatttgaag gggatatagt attagtcagc gtgcgaaatg gcttcgatta
    13621 cttagataat aaagtttgta ttgtcaaaaa ttcaatagat tattccggat tagtttgtgc
    13681 cactgttgat gaagacttag agtatcaaat ttttaacaca gagctgtttg aagaatacac
    13741 gtatgaagtc atcggaaata tatacgagaa tagcgagtta ttggaggtag agtgattatc
    13801 ttagctactg actacatcga ttctttaaaa gatgaaaatg ggaatgtccc agcattgcta
    13861 aattgtggaa ttaaaagcaa gaatagaatc attttgttac ggatatcggc tgatttagca
    13921 aaatatcttt atctgagcaa gacagcagta tattacactg cggctacacg ttatcagtac
    13981 aagggaaaaa caatcagtca ggattattat gatcgtttaa tcagtcctga tatgtatggg
    14041 aattcagaat cagcaattaa gctgtttggc gcaatagaga tatatgccga tgattttcca
    14101 aatttatggt tgaaggagga agcggaatga aatacgaaat accactaagt gaagcagacg
    14161 tccagtcaat tattaacggt cgggaggtta ataaaaaact tcctgatggt actgaattag
    14221 tcatcagaca aagttatttg aaagatatgg cagctccagt attaattgat cgttttaacg
    14281 tgactgattc tgtggtagag aaccacttga aagaatttcg atcaagtata aacgacactt
    14341 tcagattagg gagttgattg acaatgaaca ccagacaccg cagagtagca aaactaagaa
    14401 aacaggaact gaatgtacta aagacaaagt ttgaaaaaga atatggaatt tcagcagaag
    14461 aaacatataa agtggcaagt cagtgtgttg ctgatgcaag tgatgctatt cgtaagtttg
    14521 gaatttcgat attaagtgat gatcgtaaat gggaggaaaa agaatgaaac taaaagacgg
    14581 attttacgct agcagtcacg gtatcggcgg tttaatgcta gatatgccga caaagaatcc
    14641 taaaacacgt aagaaaccaa aagtcaaagt cggtgacatg gtccgctgtg aagcagagga
    14701 gttcatttat ccatttcgtg gatatgtaga acacgtctat aatcactcag cgatcattcg
    14761 cattgaaaac acgatggaat gtgataaatg gacagctaaa agcaaagaga atttagcagt
    14821 agtgagattg gtggatatgg aactaatcaa tgacaaataa aaaagccgga tcgctccgac
    14881 taacataata aaacagacaa gtttattata tcacataaaa ggagcagttt gaattgatgc
    14941 aattgttacg agaggtagat ttcaaacaga caagatgtaa tgcgagagat gtgctgaaga
    15001 actttcggcg tttggagcgg atggcaggtc gctctttgat agatattaag tcgccgatta
    15061 ttacggatat gccgaaggca ccgaagcacg gcaataaggc agaggatgca ttgattcaga
    15121 tgatggatat agaagcggag agagacgcga ttttagcggc tttgatggct cttagtctga
    15181 ttagtcgtca gatactctac tacagttttt gtgtgccaga cagcttctca aactacagaa
    15241 ttagccgtga agtgggttat tcagaaagaa gtatacaacg gatgaaatcg gaagctctaa
    15301 tagagtttgc agaagcatat aaacacggaa gaataattgc ttataaatag ggggaccata
    15361 taatgtggaa agattatgta tcgttgaaag agttgaaaaa agatcttgtt ttcaaaagaa
    15421 ttgtagaatg gtcagagagt gaattaattt tagaagatgg aaccaaaatg gaagttgtat
    15481 gtagcgaatc agattgctgc gcatgggccg aaggtgaatt caaaaatgta aagcttgatg
    15541 cagtgattac agatataaag atttttgata aaggtaaccg tctctataat ggtgacggac
    15601 atacctctta tgctgaagtc gttgtttatc ataatcgtaa tgagatcgct aaagcagaat
    15661 gcacagcgaa tgatgggaat ggcggctact attatagtgt ttgtgcttta aaggtcaaag
    15721 acaaactttg tatagtgact gacgcataaa aaaatggcgg ttttttggcg gaatgatggc
    15781 ggtttttagc catttatcag tgatattatg gtagtgtcga aagattagtg ataggtctga
    15841 gacaaaataa taataaaagg aacatcgttt tattattgtt tcacaattaa gcttcgatag
    15901 acagcaacgg aaatattaag aataaggatg tgaattttaa ctccttctaa attgttctta
    15961 ttatctatca tccgttgctg tttattgtta catacagatc gcttaggcgg tctttttatt
    16021 ttggccgaaa acctacattt tcgatggccg attattggag gaggaatagc atgttcaaac
    16081 tatctgaaat cattaagaag tccgacgttg agaaattaga gatgttaaaa aagaagttga
    16141 agaaatagca gtctcttcgt gaggctgttt tattttgctc acaaaaatag accactatcg
    16201 ggtaatagtg gtcaggaatt aaatgaaaaa gatgttaggg ttgttagcta agtataacat
    16261 cataacgctt acaaaacaat gcaagaagga taaattatta tgagaactta ctggtatgtg
    16321 tcattaaaca ataaatatcc gaagccaatg aaaggacagc ataggcgtgt agtcatgtct
    16381 gttcaaatga aggcgaagta ttcgattgta gaaatgctaa gagaagctac accaaaagag
    16441 attgatcagt ataagttggt tcttattggc attggttggt ttaaggatga acatattcaa
    16501 acaaacatga agaggtggtt gagatgaagt tatattatgt tgaaaaagca aattgttggg
    16561 tggttgaagg ctgtctgtca aagtacgcta cagatattat atctactcaa ttttcagagt
    16621 tgggattgaa ggttatcttt tgtggtgagt ttgtcagtgt gaaagaagcg gtattgccaa
    16681 tgacagcaaa gagcagacgc tcgcgtggtt caagtt
//
LOCUS       DANJMI010000057        16624 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000057 DANJMI010000000
VERSION     DANJMI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.057"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(93..1202)
                     /gene="rpoD"
                     /locus_tag="PWU81_002368"
     CDS             complement(93..1202)
                     /gene="rpoD"
                     /locus_tag="PWU81_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL1111039.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1226..3067)
                     /locus_tag="PWU81_002369"
     CDS             complement(1226..3067)
                     /locus_tag="PWU81_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL1111040.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3249..5564)
                     /locus_tag="PWU81_002370"
     CDS             complement(3249..5564)
                     /locus_tag="PWU81_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL1111041.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5749..6609)
                     /locus_tag="PWU81_002371"
     CDS             complement(5749..6609)
                     /locus_tag="PWU81_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDL1111042.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6668..7189)
                     /locus_tag="PWU81_002372"
     CDS             complement(6668..7189)
                     /locus_tag="PWU81_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDL1111043.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            7467..8228
                     /locus_tag="PWU81_002373"
     CDS             7467..8228
                     /locus_tag="PWU81_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL1111044.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8254..10845
                     /gene="mprF"
                     /locus_tag="PWU81_002374"
     CDS             8254..10845
                     /gene="mprF"
                     /locus_tag="PWU81_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL1111045.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11033..12220)
                     /gene="tuf"
                     /locus_tag="PWU81_002375"
     CDS             complement(11033..12220)
                     /gene="tuf"
                     /locus_tag="PWU81_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL1111046.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12588..12944
                     /locus_tag="PWU81_002376"
     CDS             12588..12944
                     /locus_tag="PWU81_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL1111047.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(13185..14189)
                     /locus_tag="PWU81_002377"
     CDS             complement(13185..14189)
                     /locus_tag="PWU81_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDL1111048.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            14355..15206
                     /locus_tag="PWU81_002378"
     CDS             14355..15206
                     /locus_tag="PWU81_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL1111049.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            15223..15468
                     /locus_tag="PWU81_002379"
     CDS             15223..15468
                     /locus_tag="PWU81_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111050.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            15655..16359
                     /locus_tag="PWU81_002380"
     CDS             15655..16359
                     /locus_tag="PWU81_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111051.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         4904 a         3419 c         2938 g         5363 t
ORIGIN      
        1 aaaaagcttg agactgcgta gtctcaagct ttttttcttt taaaataaac caaacagttt
       61 tactgatttc tcttctggtc aatagtccac gcttattcta agaaatcttt taattgtttt
      121 gaacgagaag gatggcgcaa ttttcttaat gctttagcct cgatctgacg aatacgttcc
      181 cgagtaacac caaatacttt ccctacttct tccaatgttc tcgtccggcc atcatctaaa
      241 ccaaaacgta agcgaaggac attctcttcc cgatcagtca atgtatctag tacatcttcc
      301 aattgttctt ttagtaactc ataagctgcg tgttctgctg gacttgttgc ttcctggtct
      361 tctatgaaat cacctaagtg agagtcatct tcttcaccga ttggcgtttc aagggaaaca
      421 ggttcttgcg cgattttcaa gatttctcgg actttttctg ttggaagatc catttccgca
      481 ccgatttctt ctggcgtagg ttctcttcca aggtcttgca gcaattgacg ttggatacgt
      541 atcaatttat taattgtttc aaccatatga actggaatac ggatcgtacg tgcttggtca
      601 gcgattgctc gagtgatggc ttgacgaatc caccatgtag cataggttga aaacttaaat
      661 cctttgcggt agtcaaattt ttcaactgcc ttcatcagtc ccatgtttcc ttcttggatc
      721 aagtcaagga actgcatgcc tcgtcctaca taacgtttag cgatacttac tactaaacga
      781 aggtttgctt ctgccagacg ctgtttcgct tcttggtcgc cttcttcgat tttcaatgct
      841 aacgctactt cttcttccgc agttaaaagg gagacacggc caatttcttt cagatacata
      901 cgaaccgggt cattgatttt tacaccggtt ggcgcagaaa gatcttctgt ttttgcttgt
      961 tcagcttttt tctcagcact cttcaagctg tgaattgatg gatcaccatt ttcatccacc
     1021 acactgatac ctgcgtcttc aatcttttgg atcaatttgt ccatttcatc tgcattcaaa
     1081 gtgaatggtg tagcaagctg attggataaa tcatcatata caacttgtcg tttgggtttg
     1141 ttttctttga tgaaagctgc tactgctgct tcatacgttt ttttatctgt tccatttgcc
     1201 ataaaagaag gccccccttg agtagctatt tggcttgttt taactgtttc gttaaattga
     1261 tgatttcaat cgtcaattca agctcaagct gtttgtttcc agttcgtctg gcttcttgct
     1321 gcatttgttt ttttaatgtt atttcatcag ctaagccaga tttgctgatc acatctagca
     1381 agtcttggat ttctcgtggc gaactttctt ctgccaccgg taaatattcg atttccacca
     1441 ccaaccgcct taaattttca tcttgcaaaa agtctaaaaa ctctgcaagg ttgaattcat
     1501 aatgttgtga gacatatgca tcgaataaaa aataaacttc tgcatatgca tcatgaataa
     1561 actgaaattc tcgttctttt aaaatattac ggacagaaac ttctttaaat gcacgatata
     1621 atagaatctg ctctgccttt tctacttgcg tcaaaggtct ctttttccgt gcagtttctt
     1681 gtctcgtaac ctgagtgtgt tgactttcct gaggttcttg acgattttgc tgatttgtcc
     1741 ttcgaagatc gcgcagttgt ttttgtatcg tctctctgcc gatctgaaac tctgttgaca
     1801 gttgagatag atacatatct tgttcgattg gtgaaacaac tcggctaagt tcaactagta
     1861 attcttcgag gtattcgatc ttgtcttttt cattatctaa atttttccct tgttttaaat
     1921 aacgtgattt aaaagtaaag actgtttcgc gcccatgagc cactaattct tggaaagatg
     1981 cttctccata cttacggaga tattcatcag gatcaagctt ttctggaacg actacgacag
     2041 aaagtgctaa tcgactgttt tgccgaagca attcaatccc tcgattggtt gcttcaaaac
     2101 ctgcgttgtc tccgtcatag caaaagacca attctttcgt cagcttttcc atttgttgga
     2161 tctgttgagc tgttagactt gtccccatag aagctaatcc agttttgata ccagcctgcc
     2221 atgcagcaat cacatccata aacccttcga acaacaaaat ttctccgttt ttccggatat
     2281 cgcttcttgc tttatcaaga ttaaataaaa cttgtctttt attgaacagt tctgtttctg
     2341 gactattcaa gtatttgggc tgatcttttt cctgcccttt ttcaggtgca agccagcgtc
     2401 ctgaaaaacc gatcgtctgg ccttgagaat tacgaagtgg aaacatcact ctttgataaa
     2461 aacgatcaga catacggcca tcatcatgcg tgacaaacaa tccagagcgt tctaacaatt
     2521 catttgaaat tgattcattc ttgaatactt gctgaagaaa agaacgctca ttaggtgcaa
     2581 aacctatgtt gaattcttca attaattcta aagacaaccc acgactttgc aaatattcta
     2641 aagctggctg tccagctttt gtatgcaata gcatgtgatg atagatttcc gctgcttttt
     2701 catgagcagc aatcagtttc cccgtagcag attcaggatt ttggacagct atagtacgct
     2761 gacgccattg atcttctaac ggtatctgtt cgaagtctgc cacttttacc actgcttctg
     2821 gaaaacttag accttctagt tcttgaagaa aagtaaacac gtttccgcct ttaccacaac
     2881 caaagcaatg aaaaatctgc ttgtcttctg caacagaaaa agacggtgta cgttcttcat
     2941 gaaaaggaca cagccccaag taatttttac cagacttttt caactggaca tattgtccaa
     3001 tcacctcaac gatattcgtt tgactgcgta ctgtttcgat aacctcttga ggaatccgtt
     3061 gagccatttt ttcaccccct gctctattga tatcttttgg gtttaacaga agatatttca
     3121 ggagaataac cggtaaaaaa tcgcactaac tgaaatcagt gcgattttaa aggacacaat
     3181 tatacatgtt tgattgtatc atatttcatt gctttttcaa gtataacaca tttctatctc
     3241 attaagcatc aatttgctac tcttttcagt aactgatttt gaatatcagc cttccagtac
     3301 aatactcgaa gaacaatatt tacgagcatg aatacaaaga agtatcctac gctctctccc
     3361 aaccctaagt ttacttccaa aattccatgg aacagcattg cagctacata tatccctacg
     3421 atcgtcgtta ctgctaggaa gatgcgcaat gggttgaagg gataacaagc ttttacaact
     3481 gccatgcaac tgatcccaat cagcaagtaa tacatcaaag tagtagtttc agcatttccc
     3541 catgcgaatg tttttccaag tagatacacc gcaatgatat tcactactac aagcaaggca
     3601 tttggtaaag cattaataag tgctgtaggc aaataacggt aagtgatttt tcttttatct
     3661 tgttcaaaag ataaaaagaa ggaaggatac ccttcaatcg ctaaatcaat cagcgtgatt
     3721 tggataggga taaacggaaa tccaattgct gtaacggcac atataatcga taaaataaat
     3781 gaataaatcg ttttgataaa aaagatgcct gagactttcg tcacattgtt gaccactcgg
     3841 cgtccttcaa acaggacttc tggcaatgta gtaaaatcag agtccaacaa taccagattc
     3901 gcaatctgac gtgttgctcc atcaccttct gccatagcaa tgctgcaatc tgcttctctt
     3961 aacgccaaaa catcattcac accgtcgcct gtcattgcaa ccgttctgcc gctttctttc
     4021 aattcattta ccagcaattt cttttgctgc ggactcactc gtccaaaaac cgtatagcga
     4081 tgagcagctt ctcgcacttc catttcttca gtaatttgag aaagatcgac atacgcatca
     4141 taattcggta atcctgcacg tctagcgata ttggagacag taaaaggatt gtctccagaa
     4201 atcactttca gatccacacc ttcctcatga agataagcta gtgtttcttg agcgtttttt
     4261 cggattggat catcgatttc aaacagagca accggctcaa caaatgctgg tttttcttcg
     4321 ttcaattgct gatttggagc gatccccaac ataagtacgc gatagccatt cgcttgggct
     4381 gtttttagac tgtccggaag ccgatcttct tccaccaaac gttcaggcgc tcctacgtac
     4441 acgactccca gttctggaaa ttccatcgcg ccccatttgc gatcagaaga aaatgcgact
     4501 ttgtttgtca catcataccg attagaaagc tcaaaataat ctctcatagc ccgcatagtg
     4561 atgttgttat cactgctttc tgccagatac gtccccataa tttctgggat ggctttctcg
     4621 tacagcggat gtagtgcttc tacccgctgg atactcattt tcccttcagt aatggtccct
     4681 gttttatcca agcataatgt atcgacatgg gccagcgttt caattgaata catgtcttga
     4741 acaaggattc gtttttttgc cagtttggtt acccctgtcg ttaatgcaat acttattagc
     4801 aataccaaac cctttggaag catgcctaac aaagctgctg cagatgcaat aacagaaatt
     4861 ttgatagaag catcgcggat gaacagtgct tccaaaaata gtatgatccc caaaggaata
     4921 atcacgaaac tagtgaattt ggatactttg cgaatcgaat ctacaagttc agaacgaata
     4981 gctttgtgag tctttgcctc gctagtcaag cggacagcat aattatctgc acctactcgg
     5041 ataacttcag ccatgacaga accgcttgtc aaaaagcttc cagaaagcaa ttcagctcca
     5101 gtttctttga caatcaaatc agattcgcct gtcaaaagag cttcatttgc ttctgcccgt
     5161 ccagatataa cctgtaaatc ggctggtatc tgttctcctg ctccgatttt taccaaatct
     5221 cctaatacca gctctttagt agaaatcgat tgttctttac cgtcccggat cactagtact
     5281 tctgattggg aaacaatcga taatttccgt acaagatttc ttgcatgtat ttcttgataa
     5341 attccaataa cgatattgac gacaataatt gctaaatacg ccatgttgga atacgcacct
     5401 acaaataaca gacaaatccc aataccaaag ttcagaaaat tgaataacgt caatatatta
     5461 tcgctgaata tttgttttgt actttttgat gcatcttcct ggtagtcatt ttgctggcca
     5521 gaagccattt tctgaactac ctcttgctgt gtaagtccat tcattttctt tttctccatt
     5581 ttccgccaac tttcaaaaat aatcacgtgt ttaagtctag cgtgttccaa gattaaaaac
     5641 aacttgtttt tataaaattt atagaaaaac acacgaatga ttttacaaaa aagatgccaa
     5701 cctttttgtt tgaatcaccc gtgtgctgtt tttattatca atctatcttt acttatattg
     5761 ttcctccaaa cggctcatcc accaacctag tgctgtaccg gctataaaaa gaacaggaca
     5821 agctagtacc ccagcaactc ccaatcccgc gtaatcggta ggaatgatca taatcgttga
     5881 agcaaaaact acacctaaaa tgaaatggaa tagcttgcta tatgcctttg agaaaatgta
     5941 atccatgatt tttgacaatc caagaacaca aagaatccca ccaattccga tcggcacaat
     6001 cacttcaagt cgtacttctt taaatccgtc agccatcgct ttatacaatc ccatatacac
     6061 caaaaaatta gaagggctca atcctgggac gatcataccc aaaccaatca atccgccggc
     6121 aatcagccat gttccaaaat tctgatcgac ttgcccgtca aataattgag cgccgtataa
     6181 taaaaacaaa taagccagaa caaaagtaac aaccatgatg atcacatcag aagtatctct
     6241 tccttttttc cctgcttctt tccataacgc aggaacagtt ccagcgatac atccgacaaa
     6301 aaaccataat atcgttgttt catacgcacc taataaaaaa ctgactaaaa aagaaagtag
     6361 aaagatgcct gtcactccgc ctagaccaac aggcagaaaa aacaaaacat tttctttgaa
     6421 attttttgta aggtgggcta aaaaagaaat cagccgctca tacatgccaa agacagctgc
     6481 taatgcacca ccgctgactc ctggcaagat aaatcctgaa ccgataaaca ttcccttcac
     6541 aaaccgtaac atccagtctt tggttgtgta ccccacttct tgattttcca ttttttgcct
     6601 tttttccact atttgcgtac tccttctttc atttaatttt attcatgatt gaacatttcc
     6661 tgctatttta cttaaagact ttttcatagg tctctattat tttttcattc attttgacaa
     6721 tatcaggata aaacatgtta cctccattat gatagagata gccgccattg taaagcaagg
     6781 actggatccg ccaatatcgg tattgtttcc cttcctcatt tcctaaaagt gggctcagca
     6841 catctttaga atacgcttct gctaattcta ctgtgttttc tccgccatga ttttttacat
     6901 aggcaatata atctttccca aaattatatg cttggacagc tgtccataaa tcgcatcctt
     6961 gtttctttgc ttctactatc atttccgcca aatatttgac accttgatca atactgtcag
     7021 ctggattatc aatgatctca gcttctccat gcagactttc cgagctctgc atgagatctg
     7081 tcccttttcc ttttgattcg gtcaatatta tcgccttgat caaattctcg tagttttcca
     7141 tatgataatt ttttgcagct gttgttattt ctatttgata gctttccact cgttttaccg
     7201 aacgataagt tcttcctcat aaaaacaaca ccgcaccaat aaccgtcaga atgatcatgt
     7261 aaaaaagaat acggataata gataatcgtt ttcgcttcat ttcatcacct agttattttg
     7321 acatcttttt ttatcttact atttctttat cttcttggct atttcttcct tgatttctgt
     7381 gtacgcctta agaaaaaatg aattttcctg tattttttga catcttacga aaatgatcgt
     7441 tgtttttcag taaaaacaat tgcccaatga ctgatataat cgttatactt tacacagtag
     7501 gtgattatgt gaaaaaagaa aagaaatatg caaaaatgcc agatgggagc gaaatttact
     7561 atgaaaagag cggtcaaggc ttccctctct ttttactgca tggcaatgac ggcagcggcc
     7621 gctttttttc agaacaggtc cctgtactcg aacgctacta taccgtgtat ctagtagata
     7681 gccgaggaca tggtcgttcg acaaacgaag catctatgtt gaattttcag ttaatggccg
     7741 aggatctgaa tacgatcatg ttgctggaaa aaatcgatca ggcagatttt cttggattta
     7801 gtgatggagc aaatctagca ctggtatttg ccagcagctt ccctaaaaaa gttcatcgat
     7861 tgattttaaa ttctggaaac acgcttgtta aaggcgttcg tttttctgcc agagtcatca
     7921 gtaatattca ttatgcatgg gtatggttac tctctttatt tcgcccaagt cttcgcaaaa
     7981 atcttttagt tatcaaactt ttgctgcatg acatcgggct tacagaaaat gatttaaaaa
     8041 aaatcaactc ccccactttg atcatcgtgg gaaaaaaaga tgtaatcaag ctaaaacatt
     8101 ctttgtatat agctaaaact ataccaaaag cttcctttgt gcttgtgaaa gaacaaggac
     8161 atgaattagc tagaaaagat cctgaacgct tcaatcgaga agttcttcaa tttctttctg
     8221 aaacataact attaggaata gaaggtgatt tatttgttaa aaaataccat acaatggctg
     8281 aaaaaccacc taggactgtt caaaacaatc tttttgattt ctgtcattgt cattattgtc
     8341 ggtgaattga tgtccatcgg taaaactcta tctatccagc aattaggaga aacgtttgcc
     8401 actatacctg tatggaaaag cggactaatg ctattgattg gtcttatctc tgtgttacct
     8461 atggttggtt atgatattat tttgaatcgg atgcttgaac aaaagcagaa tcctcgttac
     8521 ctgtttgaga caagctggct gatcaatacg atcaacaata ttgccggttt cggcgggttt
     8581 gttagtgtgg ggcttcgctc tgaactatat ggacaaaaga aagagagcag aaacgtgatc
     8641 caagcattat cgaaagtttt cttgtttttg atggctggac tttctattta cagtctgctt
     8701 tcttttttac ttgtagtctt cacgccagtt gctccttttt tgaagcaata ttggatatgg
     8761 ttgatcggcg gcggtctata ttttcctgtc gtctttttat ttactacttt caaaaagaaa
     8821 ggattccttg gtggtacctc acttaaaact caaggacagc tattgctagt ttcattgttg
     8881 gaatggaccg gtgtcctgtt tagtttcctt tctgttggtt atttaatgga aatccggatt
     8941 gatctgtggc aaacaattcc attattcatt gctgcttcag tgataggaat cgtgtctatg
     9001 attccaggag aaattgggag tttcgatgta atgatgatta tcgggttgtc cgctattggt
     9061 gtgccaagag aaatagtcgt tgtgtggatc ttgctctatc gcctgttcta ctacatcatt
     9121 ccgtttttga ttggaatcgt ctttttcttc aaaaatatcg gttccacttt tgatcagcgt
     9181 tattctggta tccctaaaca attagcaaca gaaattgcgc ataaaatcgt ggttgtctta
     9241 ttatactttt ctggaatcat gctagtactt tctgccacta tcccgcaagc ttttacagag
     9301 tttcgctggc ttcatagttt gaacccattg aaatttcatt ttattatcca atttcctagt
     9361 atactattgg gatttctttt aatcattatg ggcagaggga tcgcagcacg tgtgaaacga
     9421 gcatatttgc ctacaatctt cttgattgtt ctagctttac tttatgtttt attaagtgat
     9481 tttagtttca caccggtggt tttcttgtct attctgcttc tgatcattct tgcttctaaa
     9541 aacgaattat ttcgggaaca attgatctat tcatgggagt ggcgtacgat cgatggaatc
     9601 attattggtg cactgtcctt gctttacatc gttattgggg tatacaatct gccggatttc
     9661 cctcatcggc gtcaccattt tatttccttt ttcttatttc cttctgaaaa gatttggttt
     9721 tcgggattgt tggccatcat cgctgttagt ttcatgatcg ttctatttgt tcatttcctt
     9781 caaggagaaa agaaacagat tggcgaagcc tttaatgaag aaaaggccct gaagatcctt
     9841 acaacttatg gaggaaactc cgatagtcaa ttgatcttct taaaagataa gcggatgttt
     9901 gcctatgaaa aagacggtga accaactgtt cttctccaat tcgcttgctt caataataaa
     9961 tgtattgtaa tgggtgatcc ttctggtaag aaagaagatt ttccagaagc aatcgaagct
    10021 tttatcgaag aaacagatcg actatgctat ttaccagttt tctacgaaac aagcgaagag
    10081 attgttatga ttttgcatga attcggctac gatttcatta agatggggga agaagcttat
    10141 gtagacctga atagttttac aacttcaggg aaaaaaatga agggcactcg tgctgtcttg
    10201 aatcggatcg aacgagaagg atttacattt gatgtcttac aaccaccatt ttctactgag
    10261 caaatgagta ctttcaaaaa tatctctgat aattggttgg gttcacgaaa ggaaaaaggg
    10321 ttttctttag gctttttctc tgaagattat ctacaaagag cgcctattgc agttgtcaaa
    10381 aatacccaag aagaaattgt cgcttttgca acgatcatgc ctacttacac gaacaataaa
    10441 gtaggaacga ttgatctgat gcgatatgat tctaaaaaag caccttctgg cagtatggat
    10501 ttcttgttct tgcatttgtt tgcctatatg aaagaagaac atatccaatg gttcaattta
    10561 gggatggccc ctctttcaaa tgtcggaact tccagaaaga gttttttaca ggaaaggatt
    10621 gcatacttgg tttacgagtt tggttctcat ttttattctt tccatggatt aagagaatat
    10681 aaaagcaagt acgctacaaa ctgggtctca cgttatactc tatattctag agatagttgg
    10741 attgcatatg ttatgattgc tttgctgatt attgataatg cgccagttga gcagacacaa
    10801 ggtagaataa gtggtttgaa aaaatggata cgaagaaagt attaaaaact gcataccgat
    10861 agatgaacac tctcatgatg aataaaagag actgaagtaa aacgaaagtt catgcttcta
    10921 caatctcttc ttatacaatt gaagaacaaa gagcggtcca aacagaaatc ctgtttggac
    10981 cgctctttta ttttaaacat ttttcattac caaaatattc gagtcgcgtt tcttatgctt
    11041 cgatctctgt aacgatacct gaacctactg tccgtccgcc ttcacgaatt gagaatgtcg
    11101 ttccgttttc aacagcgatt ggatggatca agtcaacatc aatcgtcacg ttgtcaccag
    11161 gcatgaccat ttctgtatct tctggcaatg tgattgttcc tgtaacatca gttgtgcgga
    11221 agtagaactg tggacgatag ttgttgaaga atggagtatg acgtccacct tcttcttttg
    11281 tcaacacgta cacttctgct ttgaattttg tatgtggagt aatggaacct ggtttagcaa
    11341 gtacttgtcc acgttcgata tcgtcacgtt ggatcccccg taacaacacg cctacgttat
    11401 ccccagcttc cccgtaatct aacgttttac ggaacatttc tacacctgtt acaactgctt
    11461 tttgtgtttc aggtttgatc cctacgatct ctacctcatc accgacacga acagcaccac
    11521 gatcaatacg acctgaggca acagttcctc gacctgtaat tgagaagacg tcttccactg
    11581 gcaatagtaa tggtttatct gtatcacgtt ctggtgttgg gatatattca tctactgtat
    11641 ccatcaattc cataatagca gcttcagcat ctggatcgcc ttgcaatgct tttaatgcgg
    11701 aacctttgat cacaggagtg tcatcgcctg ggaaaccata ttcgctcaat aactcgcgaa
    11761 cttccatttc taccaaatcg atcaattctt catcatcgac taaatcaact ttgttcaaga
    11821 aaacaatcag gtattttaca ccaacttggc gtgacaataa aatgtgttca cgtgtttgag
    11881 gcatcggacc gtcagttgca gaaacaacta agattgcacc atccatttgt gctgcccctg
    11941 taatcatgtt tttcacatag tccgcgtgtc ctggtgcatc aatatgcgcg tagtggcgtt
    12001 tttctgtttc atattctacg tgtgcggtat tgatcgtaat cccacgttca cgttcttctg
    12061 gagctgcatc aatactagca tagtcttgag gatttgctag gccttttttg cctaatactg
    12121 tagtgatcgc agcagttaaa gtagttttcc catggtcaac gtgtccaatc gtaccaatgt
    12181 taacgtgtgg tttacttctg tcataatgtt cttttgccat aatttacaaa cctcctaaat
    12241 aatatagatc actgaaaaat ttcagctctc acctttttat tatagtcttt ttttgactaa
    12301 atcaaaaaga atacgtccga cttacttttt gtaagattcg aactaagctt attatacgtc
    12361 aatattggtc aaagtcaact aagagcactc gtctgtctta tacgcgaaaa aagtaccttt
    12421 tataaataaa aaaatgtgtt ttttgagaaa aacttcaaga tagtccttaa ctttcttttg
    12481 aagcaataag aacaaaatga ggaaaagttt aaaattcagc tgttgccaac aactttcaat
    12541 agacgaaatc ttctatttcg ttaataataa ggaaagaggt gaagtacatg aaaaaattaa
    12601 ttggcgtact tgtcgtttta ggcatatttc tcggcggaag ttttgcagct tatcattatt
    12661 tttacggcgg cgaagcttat tatacaaaaa tcacgacgaa tggcgaaatg tcagctaccc
    12721 aagcagataa cggagagaaa ttcacgactt atacctacaa gcaagcggct tataataaag
    12781 atggtgaaaa aaaacaggtg acgcttcgcg aagaaagaga acatccatta aagatgaatg
    12841 cgtatcttaa actcaaagtc aatcctcgta aaggtgtatt aagctgggaa gaaataaagg
    12901 cttctgaagt gcctaaaaat gcggcagaaa aaatcgataa ttagtaaatg gtcttaaaat
    12961 tttattagat aaaaagaggc tgggacagaa gcgtttaact ccaagaaata agaaggagtt
    13021 gcttctgctt ccgccgttta tacgtgttta gagcgtgaga caaaagtgtt ctttactttt
    13081 gtctcacgct ctttttttgt tatattcgtc ttttccgatc cttctcttga cacaattcat
    13141 ttaactgatc actggtactt gaacacctaa tattctagtc atttttataa acgattcaat
    13201 acttcatcca catttttgat catcacggat atcgtaccat ccggattatt cacaaattca
    13261 atcaaatctg ggttgcgata tacatctact ggtacgatca gttcgatccc gtttgatagc
    13321 ttgaattttt gttttccaaa ttttttctca gaaatttctt gtacttcttt tacaggaggt
    13381 gcttctggga caaatccttg ctcttgtact tcttctttaa aagccatttt tgccgtgatg
    13441 ttttccttga agactttttc cgcaatcatc tcatgatcca accgaccttt ttcttctatc
    13501 gtatcataga cagcttcttt caaatcagcc atgatatcaa attcttctgt ctcaaatttt
    13561 tttccaagat gcttggctac tttttggatc acttttacat tttcttctaa agaaggtgca
    13621 ggaacacttt cgatcactct tccagaaaaa taaagctctt ttttgccaga aaactcatat
    13681 tttttttcaa ttaattcgta ggtccaatca gtcaaattca cagcgaatgc ttcatctgct
    13741 ttttgtgttt tcgcccctag aattgctcga ttcaaaatca agcggttgtc gatccctgct
    13801 tcgtctgctt caacaaagtg agtgtaagcc tcacgataat tgactttcaa taaagataag
    13861 tacatcactg tatctaattc atacaagaca acaaatacat ctgcactagg tgcttcttca
    13921 ctttcttggt agatatcgaa ccaccgctga acgattgctt cacttgcttc cacaaaattt
    13981 tcttgagcga ttttaagtga ttcaacgatc atgctttctt ctgctagttg tccagttttt
    14041 gtttgagcag aagagatttt ttggatcttc ttttgaagaa aatcacgaat atattctttt
    14101 gtcagatcca gctctttttg tgaaaagact ggagaaccag tttctctgtc aataatatgc
    14161 agaattgctt tttttaaata gatatccatt ttctcattcc cttctgtctt agtttacgga
    14221 aaaaaaatca aaaataccag aatcaaaatc attttctttt aaacaaaaat ttcaagagta
    14281 gaaacaaaat ggattctttt tttcgaaaaa tgcgctacac ttatagttga acgaataaaa
    14341 aaggagctga aagtatgaat tcattaacaa gcacttatcg tctatcaaat ggttacgaaa
    14401 tcccggtagt aggattcggc acatggcaaa cacctgatgg ggatgtcgct gtttcctctg
    14461 ttaaagaagc gctggctgca ggttatcgcc atatcgatac tgcccaagga tacaagaatg
    14521 aagaaagcgt cggccaagcg attaaggaaa gtggaattcc tagagaagag atattcttga
    14581 caacaaaatt atggaatgca aaccacagct atgaacttgt gatgtcttct tttgaagaat
    14641 cattgaagaa gctccaaaca gattatttag atctgttttt gatccactgg ccaaaccctg
    14701 ttgcttttcg tgataattgg gaacaagcaa atgcagatac atggcgagca tttgaagaac
    14761 tgtatgaagc aggtaaaatc aaagcaatcg gtgtgagcaa tttcttaccc caccatctag
    14821 atacactagc aaaaacagca aaaattatgc cgatggtcaa tcaagttttc cttgcaccag
    14881 gcgaattaca gacaactgta gttgagtatg caaaaaaaca tgatatgatc ctcgaagcat
    14941 atagtccatt aggcactggt aaaatcttcg atgtgcctga aatgaaacaa atcgctgaag
    15001 cacatgacaa gacgattgca caagttgctt tgcgttggtc gctgcaacac gaattccttc
    15061 ctcttcctaa gtcagtgaca ccaagcagaa tcaaagaaaa tactgaatta tttgattttg
    15121 aattaacaga agaagaaatg aaacaaatcg atcagttaga tggtgtagtc ggcaaagcaa
    15181 aagacccaga tactacccaa ttttaagtcg aaaggtgatt ttatggatcc aatcgaacgc
    15241 ttgaattctc tctcggaaga agttacccag acttttcatt ccgattttgt ctttttgatc
    15301 gatgcagaga aaatccaaca ttttcctgca agaaactgga cacatgatca aatcatcgaa
    15361 gaactgaaaa aacggttcga tcattcgcta atggtcaaac cctggcacga acatgaagtc
    15421 atttattcac ctgaactacc tgtttttgcc ttgataccga aaaaatagaa agaatggaac
    15481 ggattatgta tatatacata atccgttcca ttttttattt ttctttcaaa aaacgggaaa
    15541 atacgcaaat ttttctgttg taatttgcgt atacgcaagt ttttttgcat tactcctttt
    15601 catttggctg tttttttctt ataatcgaaa gggtatgagc gaaggagaga acatatgaag
    15661 aaaaaacaga aacaaaaatt attaaatcaa ttccgtcctt cattggatgg tgtaagagga
    15721 caattattcc gttctctaga agaagagagc ttgcgacgtt atggtttgcc gttacaagtc
    15781 atgcttgatc ccaaaaaccg tcaaatctta acgatcggat tagctgggaa aacaaatgaa
    15841 gatacacgga tcaacgtgcc aatcgatgac aacttcaata ctgtattaaa gagaattcgt
    15901 tcaggagaaa acgggatttt cgaacgattc cgtgacaatc ttcttattga aatcgtgtct
    15961 tactggaatg atcaacgatt gaaaaacgat gaaccaactg cgcaagctgt tgcgacacca
    16021 gttacagaag aaacagtcag tgtgccagaa gaaaaaacgg aaaaaccaaa agcggacaca
    16081 actgaaaata cacgctcaac tgatacgaat gccgctttaa gttttgctga tttttcaaaa
    16141 gccgttgctg agtatcctaa attttacaca gaacaaaatg aacatgaagt agttattaat
    16201 gaaaaaaatg ctgatgaacc tcgtaaatta gcaactattt ctatgactac tgaaaatgag
    16261 ttcgttatcg aaaaagcact cgaacggaaa tacaaagtca aattgacttt gattcctttg
    16321 atcgaacaat ttgcagcgac agcgattact gatcgataaa aattctattt gtcataaaag
    16381 gctgggattt aaaatcccag cctttttagt catataataa tttctacagc aagtaaaaag
    16441 aaattccttc taacatcagg ctcaaatatc agcttgacct gtccaacaaa tcacctatga
    16501 tcctacaatg ttaggcgact tctcttctac ctgaagatat cttggagttg cctgatttct
    16561 taagatgcct aaccttaact ataaaaaatt ctaataataa acatattttc tgatagagaa
    16621 aaaa
//
LOCUS       DANJMI010000058        15900 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000058 DANJMI010000000
VERSION     DANJMI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.058"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(52..522)
                     /locus_tag="PWU81_002381"
     CDS             complement(52..522)
                     /locus_tag="PWU81_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL1111052.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="PWU81_002382"
     CDS             complement(681..1139)
                     /locus_tag="PWU81_002382"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL1111053.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="PWU81_002383"
     CDS             complement(1310..2455)
                     /locus_tag="PWU81_002383"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDL1111054.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="PWU81_002384"
     CDS             complement(2468..3385)
                     /locus_tag="PWU81_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDL1111055.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="PWU81_002385"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="PWU81_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL1111056.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4477..4647
                     /locus_tag="PWU81_002386"
     CDS             4477..4647
                     /locus_tag="PWU81_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111057.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4862..6121
                     /gene="lysA"
                     /locus_tag="PWU81_002387"
     CDS             4862..6121
                     /gene="lysA"
                     /locus_tag="PWU81_002387"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL1111058.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6108..6908
                     /gene="dapF"
                     /locus_tag="PWU81_002388"
     CDS             6108..6908
                     /gene="dapF"
                     /locus_tag="PWU81_002388"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL1111059.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            6911..7792
                     /locus_tag="PWU81_002389"
     CDS             6911..7792
                     /locus_tag="PWU81_002389"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL1111060.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7789..8505
                     /locus_tag="PWU81_002390"
     CDS             7789..8505
                     /locus_tag="PWU81_002390"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL1111061.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8511..9206
                     /gene="dapD"
                     /locus_tag="PWU81_002391"
     CDS             8511..9206
                     /gene="dapD"
                     /locus_tag="PWU81_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDL1111062.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9222..10223
                     /locus_tag="PWU81_002392"
     CDS             9222..10223
                     /locus_tag="PWU81_002392"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL1111063.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10220..11581
                     /locus_tag="PWU81_002393"
     CDS             10220..11581
                     /locus_tag="PWU81_002393"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDL1111064.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11541..12692
                     /locus_tag="PWU81_002394"
     CDS             11541..12692
                     /locus_tag="PWU81_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDL1111065.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12667..13836
                     /locus_tag="PWU81_002395"
     CDS             12667..13836
                     /locus_tag="PWU81_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL1111066.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14011..15390
                     /locus_tag="PWU81_002396"
     CDS             14011..15390
                     /locus_tag="PWU81_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL1111067.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15671..15900
                     /locus_tag="PWU81_002397"
                     /pseudo
     CDS             15671..15900
                     /locus_tag="PWU81_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5202 a         2803 c         3384 g         4511 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaagcgaaaa tattttttca tcactaaata gtgttgaatt tatgttttta tgtataaata
     4681 gcttctaata gttcgggtat tttgttttta taaccatttt tattcttatt tttaaaaaaa
     4741 tatttcgaaa tatactagac aattcagaaa attttgttta atattattcc aataaaattc
     4801 aaatctttat caaatgaaat tccaataaaa acggaaaaaa agtgaaaaga ggaatgaaaa
     4861 aatgacagaa ttgataattg gcggagtcac agcaagcaca ttggcagaaa agtataagac
     4921 acctctgtat gtgtatgatg aagaaaagat gagacaaacg atgctgtcat tcaagcgagg
     4981 ctttgtatcg aaacgttttg atacaaaagt gctttacgcc tctaaagcat tccaaacgat
     5041 tgagatgctg catttagttc gtgaatatgg attcgggcta gatgttgtca gtggtgggga
     5101 aatctatacc gctttgcaag ctgattttcc tagagatact atctacttcc atggtaacaa
     5161 caagacgcca gaggaactga tctatgcact tgaaaatgat ttgttgcata ttgttgccga
     5221 taatttgatg gaagttgaat tattagcaga attaagccaa cgctatttaa aaaaaatctg
     5281 tatcatgctt cggttgaatg tcggagtaga agcacatact catgaatata tcgtcactgc
     5341 gcatattgat tcaaaattcg gcatgagcta tgaaagtaaa gagtgccaac aagcccttca
     5401 gttgataaaa gaaagcagct atctagagtt agaaggattt catgctcata tcgggtcaca
     5461 aatttttgat gttactgctt ggttagctga aatcgacaaa ttgacgaatt acttaaccga
     5521 ttttgaccaa cctctttcct tgaatcttgg cggagggttt gggattcgtt atacacaaaa
     5581 tgatcagccg atcccagtcg aagaggcact aaaaaaactg attgaatata cggaagaagc
     5641 tttggaaaaa cgaaaactga ccattcgtca attactgatc gaaccgggta gaagtattgt
     5701 tggagaagct ggtacaacac tatacacgat tggtttcatc aaaaagacac cacataaaaa
     5761 atattacttt gtagatggcg ggatgacaga taatatccgt ccagctctgt atcaggcaga
     5821 atacgattgt gatttagcag caaaactgtc ggaagaaaaa acagagaaag tgacgattgc
     5881 aggaaaaatg tgtgaatcag gcgatgtggt aattaaagaa gcttatttgc cgaaagcaga
     5941 atcgggtgat cttttagctg tttattccac gggagcttat ggatactcga tgagcagcaa
     6001 ctacaatcga gcgacacgac cagcagttgt ttttgtcaat aatggccaat caagactgat
     6061 tgttcgcaga caagcatttt ctgatctact aaggggggaa gtttcctatg atccttcata
     6121 aatataatgg ctgcggaaat gattttatct tattagatta cgcagaagac acagattata
     6181 gtcaattagc cgcttcttta tgcacaaaag aaaaatatga tatagacgga ttgatcgccg
     6241 taaaaatcga tccgttggaa atgatctatt acaacaaaga tggcagccgc gcgccgatgt
     6301 gcggaaatgg cattcgctgt tttgctcgat acgtaaatga tcagaacatg atgaatgaac
     6361 aggaattcaa cgtagagaca ttagcaggca caagaaaaat tacggtgttg gatccctcac
     6421 ctttttattg cagcgtagat atgggagtgc cagatgattc tccggaaaaa atcaaagcta
     6481 atcaaactta tccaataaaa aaccaggaaa tagagatcaa tggtgaaaaa gtaacgatca
     6541 ctagtttatt tttaggaacg attcataccg ttgtttttgt tgaaaatgcg cgtgaggaag
     6601 tctggagaaa aagaggtgaa ctgatctgtc atcatccttt gttcaaagaa aaaaccaatg
     6661 tcaactttgt agaagtagtc aatgaacaag aattgatcgt ccgtacctat gaaagaggag
     6721 taggttggac acttgcttgc ggtacaggtt gctgtgcctc ttatgtggtt gctaaggaaa
     6781 ctgggaaaat ttcagcagat caggtagttg ttcacttgga acaaggagac ttgatgatct
     6841 ctggtgagga tgcgatacaa atggctggcc cagcagttca tgaatggaca agaatattgg
     6901 aggaatgaag atgataaaag gttcgatcgt ggcgctgatc acgccgatga atgaagaggg
     6961 atcagtagat tatgctggtt tggaaaaatt gatccaattc catttagatg aacaaacaga
     7021 tggattatta gttttaggta caacaggaga atcttctacg ctcacacaat ctgaggagga
     7081 gcagatcctt caactgacag taaagaaagt ggctggccgt gtgccggtga ttgcaggagc
     7141 tggaacgaat aatacgaaag aaacgattga aaaagcgaag cattttgctt ctttgggggc
     7201 agacgcactt ttggtcatca ccccttacta caacaaaaca agtgatgcag gactagctgc
     7261 tcattttaca gcaattgcag aagcctcacc gattccgctt attttgtata atgttccttc
     7321 tagaacaggc atgtcgattc caatccatgt attagtaaat ttagcagaac atcctaatat
     7381 cattggtttg aaagaagcct caggagatat ggcttatgtg atggatgccg ctcggttgat
     7441 tggagaagaa ttcttccttt acagtggaaa tgatgatttg atcctaccag taatgagtgt
     7501 aggaggaagc ggtgtaataa gtgtatgggc gaatatccaa cctaaaatag tccatgaatt
     7561 agtaaaagat acgcaggacg gaagatggca gcaagcaaaa gaaaagcaat taaatgcact
     7621 tgaactgatc catgccttgt tcagtgagac caatcctatt cctgtcaaag cagccatgtc
     7681 attattagac ttaccttccg gacctctacg cctgccttta gtttctcttt caaaagagaa
     7741 aaagaaacaa ttagctcagc ttttgttaaa agaaaggacg gttgaaaaat gaaaatcgga
     7801 ctgattggca gtggacagat gggaaataga gtcgaagaag ttgcacaaga aagaggagat
     7861 acggtccttc ttttacggac ggcacctaaa gaagcaacta cgcttgcttt tacggaactg
     7921 ccagaactgc ttattgattt ttctcatcca gataatttag agaaaatcct ttcgtatagc
     7981 ttatcatatc aacttccgtt agtcattggc acgacaggtt atacacaatc acagttccaa
     8041 gaaataaaag aacatgccaa acatgtacct gtgatgtata gtgctaattt ttcgttcggc
     8101 atcatggtga tgaatcaact aatcaagcaa gcagctgcaa tgttgcaagg ctggcagatc
     8161 gaactgattg aaaaacatca tagtcgtaaa aaagatgcac catctggtac tgcaaatatg
     8221 ctgcttcaaa caatccaaga agtgcaaaag ctgcagcccg tatacaactg gcaagggaaa
     8281 aaaagagaag aaaaccaaat tggtgtacac agtattcgtg caggttcttt acctggtgaa
     8341 catgatgtat tgtttgctgc tactgatgaa atatttacga tcaaacatga atctttttct
     8401 aatcgaatct ttgctgcagg cgcagtagaa gcagcggact ggttgaaaga tcagtcacca
     8461 ggttattaca aattagaaga tttattaatg gacaaagggg tgtaaaaaac gtggaagctc
     8521 aagaaatcat ccgctacatt caaacggcat ctaaaaagac gcctgtgaaa gtctatctga
     8581 atgtaaccga gccaatcgaa tttaaagaaa gtaaagtttt tggtgaagga agcagcttcg
     8641 ttgtttttgg tgactatgag acgattgctc cggttttaga agctgaatca gaaaaaatca
     8701 ttgaaatcga aatcgaaact gctgcaagat acagcgcagt tcctttgcta gatattaaaa
     8761 aaattaatgc tcgtatcgaa ccaggtgcta ttatcagaga tcaagtgtca atcggcaaca
     8821 atgccgtgat catgatggga gcgattatca atattggtgc agtaatcggt gaaaacacga
     8881 tgattgatat gggtgctgtc ttaggtggac gagcgacagt gggcaaaaac tgccatattg
     8941 gtgcaggagc tgtcttagca ggtgtgatcg aaccagcatc ggccaaaccg gttatcgtag
     9001 aagacggcgt actggtaggt gcgaatgcag tgattgttga aggtgtccat atcggaaaag
     9061 atgcagtcgt ggcagcagga gcagtggttt tagaagatgt ggcagcagaa actgttgtcg
     9121 gtggaatacc tgcacgtgta ttaaaaataa gtgatgatca gacaaaagaa aatacagcat
     9181 tgattgctgc gttacgcgaa ttatagagga gatggagaaa aatgcgcgaa atagatacct
     9241 gtattgtggg ggcaaccgga ttagtcggcc aaacgatgct caaagtgtta gaagaatatg
     9301 attttccagt aggacgattg aaattattag cttcaaaacg ttcagccggt aaagtttgtg
     9361 tattcaagaa aaagaaacac actgtacagg aattgacaga aacttcattt gaaggatatg
     9421 atttagcgct attttctgca ggtgcttctg tatccaaaga gtttgcgcca atcgcacgaa
     9481 gtcagggact atttgtcata gataattcct cagcatggcg ggaagattcg gatattccat
     9541 taatcgttcc agaggtcaat ctggaagact atacattgaa tcggctgatt gcaaatccaa
     9601 attgttcaac cattcaagcg gtacttccgc ttaaagcttt acaagaagcc tttggtatcc
     9661 agcgagtcaa ttattcgacc tatcaagcag tttctggttc cgggcaaaaa ggcattgaag
     9721 atcttcatcg tacgcaaaat gacgaggaac cagttttgta tccttataat attagccaaa
     9781 cggtgattcc ggaaatcgat cagcctttgg aaaatgggta tacaaaagaa gaacagaaaa
     9841 tgataaatga gacccgtaag attctgcatc agcctgcttt gccagtatca gcaacctgcg
     9901 tacgtgtgcc aatcgaaaat ggacatggag tctctgtggc agttcaactg caaaaagagt
     9961 ttacgacgga agaaattaga gagtgtctca gccagtttcc agggattcaa ttagaagatg
    10021 atctagcagc acataaatat ccgacgagca ttcttgctag aggaacagat atggtttatg
    10081 ttgggaggat cagaaaagac acttctttag agaacggatt gttgttctat acgactgccg
    10141 ataacatacg caaaggagcc gctgcgaatg ccgtacagat tgctgcagct ttaatgcaga
    10201 aaatagcgga ggtggcaaga tgagtaatac gctgaaagta gcgaaattcg gcggaacatc
    10261 gatgtctcat gaagagcaat tcaaaaaagt ccgtacaatt gttctttcag atcccaatcg
    10321 ccaagtgatc gttgtttctg ctttaggcaa acgtacgtca gatgatgaca aagtaacaga
    10381 cttactttat ttgatttatg cttaccttca gcatcatgtt gattggacac ccctttggaa
    10441 aaggatttgt gatcgtttca ttcaagtaag agatgttctt gccctgcagt tacctattga
    10501 agatgaacta gcacgcatac agaaacaact ggaagacgaa aagataactg aagattattt
    10561 agtaagtaga ggtgaatatc tgactgctca actgatggct gagtatcttg gttaccagtt
    10621 tattgacgca aaagacctga ttttctttga ctatgatggg aaaatcgatc aggaaaaaac
    10681 agcacaggaa atcacaaaag catttaaaag atatccgaaa atggttgttc cagggtttta
    10741 cggggcgaac cctgcaggaa aagaaaaatt attgggccgt gggggaagcg acattaccgg
    10801 cgcgatactg gcgaagatgt tgcaagcgga aagttatgag aattggacgg atgtttcagg
    10861 aatcatgatg gctgatccgc gaatcatcga tcatcctaaa atcatcgagg aaatctcttt
    10921 tagagaactg agagagatgg cttatatggg ggctaacgtt cttcatgaag cagctgtttt
    10981 cccagttcaa gaggctgata tccccatcca aataaaaaat accaatgatc ctttcgctca
    11041 agggacaaaa atatcgaatc acgaaataga aaaggaaaat ggactgacag gaatagctgg
    11101 acgcaaggat tttctttcca tcactttatt caaacgtcat atgtccgatg aaatcgggtt
    11161 tatctggaaa gcaatgagca tttttgctaa acatgagata tcaatcgaac atattccttc
    11221 agggatcgac aatatcggag tgatcgtctc agcagaagcg attgccgata agttatttct
    11281 catcacgaaa gaattaaaag aaacacttgg cgtggaagaa atcgtcgtga tcgaagactt
    11341 agctttgatc tctgtcgttg ggggtccgca taaagaattg attggattat ctgggaaagt
    11401 gttatcgata ttgaacaaat tagaaattcg cacatcgatc ttatcacaag gagcacagga
    11461 gttgaatctg atcatcggtg taccgaataa tcaatatgaa acagtagtga agggtatcta
    11521 tgaagggatg gtgaatacgg atgctgaaag aatatcgaat ggcactccat caaattcctg
    11581 aaatcgggtt tcatgaattc aagacaaaaa aatatttata tgaacaagta aaaaacagcg
    11641 aggccgttat ccatgaaatc ggcgaaacag gactactcct ttattttgat aatcagcaag
    11701 aagaaacgat tgcttttcga acagatatcg atgcactgcc agtcaaagaa gaaacgaatc
    11761 tctcttttgc ttctaagcat gaaggttata tgcatgcatg tggtcatgac ggacatatgg
    11821 cgatgctttt agggttgact gactttataa ctaaacacat tgaggatctt atttataatg
    11881 tagtactgat ctatcagcca tcagaagaaa tttccggtgg cgcacaaagc gttattcagt
    11941 caggattgtt ggataaatat gaagtacaag caatatttgg ctttcatcta tggcctggac
    12001 tagctaaggg agaagttttc tctcgaccag gacctttgat ggcacaaagc agtgaaacag
    12061 acattatcat taaaggccag tctgcacata ttgcttctag tgaaaaagga atcgatagtc
    12121 tggaggcagc tgtacgattt ttgaagaatg tttatgattt tgaccggcaa ttaccggcag
    12181 agtggaatca tctaatgaaa tttggccaaa tccaaggtgg aacgatccga aatgttttag
    12241 ctggagaagt tattgtatcc ggaagtatcc gttcttacag ttcagatatg caaaattatt
    12301 tgaagagggg attagaccat ttagcagaac agtttcactc tacagctact atttcttttc
    12361 gctataatga cggctatcca gctgtaatca accatagagg cttatatgca gcactgttag
    12421 attcaaacaa actacatgaa ttagatgctc cagttcttca agccgaagat tttggcatat
    12481 atacgcaaca atatccatgt gtatttttct ttttaggtgt cggtgatacc cctgctttac
    12541 atgatccgac gttcgatttt gatatggatg ttttagaaaa tggtgtagaa tggtacaaaa
    12601 cgattctttt tacagaaaat cttcttgtaa aaatcgatag cagttcaaaa gaggagcgag
    12661 taaataatga tgaatttatc caatcgattt agtcctaggc tttctagaat agaagtatca
    12721 aaaatacgac aattcgatca gcaaatatcg tcgataccag atgtgatcaa actcaccctg
    12781 ggagaaccgg attttccaac tcctgaacat gtcaagcaag caggaatagc agcgattgaa
    12841 gaagactttt ctcactatac cggaatgaga ggattagagg aactgcggga agcggcttgt
    12901 atatttcagc aacagcgtta tggattaact tatgatccgc aaacggaagt tttgacgaca
    12961 gttggtgcaa cggaagcgat tgcctctgcg ttgttatctg ttttggaaga aggagacaag
    13021 gttcttattc cagcgcctgc ttattcagga tatcagccat tagtggaact agctggtgct
    13081 gaattgatcc cgatagatac ttctgacaca gggtttgtct gtcagccgga acaatttgaa
    13141 cgagcatttg aacaatatgg atcagctgtc aaagcagtca ttttaaacta tcctaacaat
    13201 cctactggga cgacattgtc tgcaaaacaa ttgaaagcaa tcgctgaggt tttaaaaaaa
    13261 tatccggtat ttgttataag tgatgaagta tatgctgaat tgacttatag tggaacgcat
    13321 atgtccatag ctacttatct tccagaacaa acaatcgtga taagcgggtt atccaagtca
    13381 catgcgatga ctggttggcg agtgggcttt atttttgctc aaaaagccct tattgacgaa
    13441 ctaattaaag tccatcaata tctggtgact gccgctacaa cgatgtcgca aaaagctgcg
    13501 gctgaagcgc taataaatgg ggtgaaagat agcgagaata tgaaagagca atatcaaaca
    13561 cgaagagact atctggtcca gcaattggcg cctttaggat ttgaagtgac ccagccaaac
    13621 ggtgcatttt acttgttttg caaactccca gctgccattc aaactgactc ttggcagttt
    13681 tgtgtagaat tagcggaaca ggcaaaagtt gcgtgtatcc caggaattgc atttggccca
    13741 gaaggagaag gttatatccg gatttcttat gctagcagca tggaaaaatt acatgaggcc
    13801 tgcaaacgga tcaaagactt tttaacagaa cagtgattca tagattttat caaaaaaaca
    13861 atcgttaatt ttgcggtata ttctgcaagg agacgattgt tttttaaatt tagttcaaat
    13921 gaacgcgttt cttttgattc ataactaatg taatgttaag tttaaattgt ttagaatgat
    13981 tttaaaagaa tattcaagga ggtttcatca atggaaaaaa tcaaagttgt cattgtaggt
    14041 gcatctcatg gagggcatca atcgatcttg gaactattga cacgatacgg tgaaaatgta
    14101 gatatcacgc tatttgaagc aggagactat atttccttca tgtcatgtgg tatggagctg
    14161 tatttagaag acaaggtaac agatgtaaat gatgtacgta atttcagacc agaaaacttt
    14221 ccgcaatcca atgttcatat tttaaataat cacgaagtaa aaacgatcga tgccgataaa
    14281 aaaacagtaa cggttactag cgcatcagat aacgaaacaa aagaatatcc ttatgacaaa
    14341 ttgattttga gttctggtgt caaaccaaac aatctgccag taccaggagc agatctagaa
    14401 aatgtctact tgatgcgtgg atacaattgg gcaaccaaaa tcaaagaaaa attaaatgat
    14461 ccggctatta aaaaaatcgc tgtcgtaggt gcgggttaca ttgggatcga agcagcagaa
    14521 gcaagccgaa aagctgggaa agaagtcgta ttgcttgatg tgatcgatag acctttaggc
    14581 acttatttag atgctgagat gacagatatt ttagaacaac acctaaatga aaacggtatt
    14641 gaagttttga caaatgcaaa aatcgaagct tttactggta acggaaaagt tgaggcaatc
    14701 aaaacaagtg aaaaagaaat cccagcagat ctagtcattc aagctgccgg tgtgaaggca
    14761 aatacagaat ggttaaaagg gatcgtcgac ttagatgaac gtggctggat ccaaacaaat
    14821 gaatacttgc aaacgaatct tccagatgtg tatgcagtag gagatgctac acttgcttat
    14881 tctatcccag cacgcaaaaa aatgccgatc gctttagcga ctgttgcacg tagagaagca
    14941 cgttatgtag taaaacatct ttttgaagaa gtaccaaaca aaccgttcgg cggagtcgta
    15001 ggttcctctg cattaagtgt ctttgactat cattttgcag caagcggatt gaatagcttt
    15061 acagcagcaa aagcaggagt agatgtacga gtgtcttatt atgaagatac gttacgacca
    15121 aaatatgtac cagaagaaca aggaaatcca aaagtgtcca tccaattatt ctcggatcca
    15181 atgacccatc aattgctagg aggagcagta ttatctacct atgatgttac aggtcaagga
    15241 aatgtattgg cactagcgat ccaaaacaaa ctgactttag aagaactagc agaagctgac
    15301 ttcttcttcc agccaggctt tgaccgacaa tggagcatgt tgaatctagc cgctcaacaa
    15361 gcgttaggag aagaaccatt tgtagaataa gaaaacgaaa tatttgaaaa gaggttgtga
    15421 aaaagctgcc ttgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
    15481 aacttggctg gacttattac cgcagatgca agcttttgaa caccgtttat tttcaaaaaa
    15541 gggactgtga cacgatttgt gtaccgaccc ccaaaagtta gaccaaaaaa tctaactttt
    15601 ggaggtcggt attttttatg gttaaatata cttttgaact taaattaaag atcgttcatg
    15661 attatttgga tggcaaaggc gattggaccg taaaaatcca gatcaagaga ttgaagcgga
    15721 aatgcttaaa attcgggaga agcacaagga ttatggctgt ttacggatga ccaatgaact
    15781 aagaaaccgc ggtttctcta tcaataaaaa gaaagttcag cgcttgatca agaagctagg
    15841 aataaaagta acggcataca cgcgtaaatc acgtcgctac aattcttatc gaggaaaagt
//
LOCUS       DANJMI010000059        15442 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000059 DANJMI010000000
VERSION     DANJMI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.059"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            104..454
                     /locus_tag="PWU81_002398"
     CDS             104..454
                     /locus_tag="PWU81_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HDL1111068.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            553..1083
                     /locus_tag="PWU81_002399"
     CDS             553..1083
                     /locus_tag="PWU81_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL1111069.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            1237..2388
                     /locus_tag="PWU81_002400"
     CDS             1237..2388
                     /locus_tag="PWU81_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL1111070.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(2497..4536)
                     /locus_tag="PWU81_002401"
     CDS             complement(2497..4536)
                     /locus_tag="PWU81_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HDL1111071.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGEKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKDDDDSATKRASELLQYLNQNYQ"
     gene            complement(4594..5151)
                     /locus_tag="PWU81_002402"
                     /pseudo
     CDS             complement(4594..5151)
                     /locus_tag="PWU81_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            complement(5690..5896)
                     /locus_tag="PWU81_002403"
     CDS             complement(5690..5896)
                     /locus_tag="PWU81_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111072.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(5912..6526)
                     /locus_tag="PWU81_002404"
     CDS             complement(5912..6526)
                     /locus_tag="PWU81_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDL1111073.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(6673..7356)
                     /locus_tag="PWU81_002405"
     CDS             complement(6673..7356)
                     /locus_tag="PWU81_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL1111074.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(7465..7923)
                     /locus_tag="PWU81_002406"
     CDS             complement(7465..7923)
                     /locus_tag="PWU81_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL1111075.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(8007..9770)
                     /locus_tag="PWU81_002407"
     CDS             complement(8007..9770)
                     /locus_tag="PWU81_002407"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL1111076.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(9991..11451)
                     /locus_tag="PWU81_002408"
     CDS             complement(9991..11451)
                     /locus_tag="PWU81_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111077.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(11659..12585)
                     /gene="glsA"
                     /locus_tag="PWU81_002409"
     CDS             complement(11659..12585)
                     /gene="glsA"
                     /locus_tag="PWU81_002409"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDL1111078.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(12608..14035)
                     /locus_tag="PWU81_002410"
     CDS             complement(12608..14035)
                     /locus_tag="PWU81_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1111079.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(14066..15280)
                     /locus_tag="PWU81_002411"
     CDS             complement(14066..15280)
                     /locus_tag="PWU81_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDL1111080.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         4413 a         3089 c         2658 g         5282 t
ORIGIN      
        1 tttgtcacaa tcctttttgt ttttttgaat aatcgctttt tcttctcttt ttcttatata
       61 ctataagtag taaataattt gaaaaaaaga aaggtgactg attatggcgc aaaatcgtat
      121 ttttacgaca agttttgcaa gcgtttatcc tatgtatgta gcaaaagcgg agagaaaaga
      181 tcgtataaaa gaggaagtag atgaaatcat ttgttggctg acgggttatt cccaaacaga
      241 actagaatat caactacaaa aacaagtgac tttcgaacaa ttttttgcgg aagcccctgt
      301 ccccaatcct tcacgttcct tgattacagg tgttgtttgt ggtgtacgag tagaagacat
      361 cgaggataac ttgatgaaag aaattcgtta tttagataaa atgatcgatg agctagcaaa
      421 aggcaaaaaa atggaaaaga tcctcagaag ttgatttttc cagagatatt cttctccttc
      481 gctcattttg taacatttaa tttggtaaaa tgactagttc acgttaaaat aagtcaaagg
      541 aggttttctt atatgtttga gacgattaaa caaaatattc aaaaatacgc cttattaaga
      601 gctgctgttt atattatttc tgggattgcg attgtgctaa acccttctgc tgtttttcat
      661 tttatcggtt atctgatcac cgcctatctt gttttgcttg ggttgttgaa tctttttgag
      721 gcttaccgtt ttaaaaggaa aacaggttct tggagtatcg gtctgttcag tggtcttttt
      781 ttcctgatct tagctctgat cgtctatgct tttgcacctg caatcgtttc tatcctgccg
      841 attcttcttg gattagctgt tatcatcaac agcttgttcc agttgttctt cagcatgaat
      901 acaaaagcta aaagttggag tgtttatagt agtattctgt tgttgattgg cggattcgtc
      961 ttattgttca atccattcaa aagtttgatg cttctattcc aaatattcgg ctttattttg
     1021 attttcatgg gcatccagga gattatcaat ttcatcagaa taagaaaaat gactatacgt
     1081 taatcatttt tagttattaa aatagaccgt ataaaaacca acaatccaaa aaaatctttt
     1141 ggattgttgg tttttttgtg agtatatttt cctatttttt caataatgta tgttatgcta
     1201 agttcgggct atattttata caaaggaatg atgagaatgg aatttattgg tattttatgc
     1261 ctgatattga tttcaacaac gattggctca catatctcgc ggagattagg tatcccagca
     1321 gtgattggac aattacttgt tggtgttttg ctaggccaag ctggattgaa ttgggtcaaa
     1381 cctgatattc tagtccatga cttttcggag attggtgtgt tcttattaat gtttttagct
     1441 ggtctggaaa gtgatttatc tcttttgaaa aaatatttcc gacctggtat gttcgttgct
     1501 ttgcttggga tcatcttccc ggttgttttt ggctggttga caggcaaagc attccaagtt
     1561 tcgggaaatg aagcgatttt ctttgggatt attcttgcgg caacttctgt cagtatttca
     1621 gtagaagtat taaaagaact aaacgtagtg aatacaaaag aaggatcaac aattttgggt
     1681 gcttctgtgg tagatgacat tttagtcgtt ttagttctaa gtttcagcct atcttttcta
     1741 acgggggaag cttctagcaa tgcttcttta ccgcttgttt tgattgaaca gctaatttat
     1801 tttgttttta ttttcttatt agtcaaatgg attgcgcctt tcctactttc atttgcagaa
     1861 aaagtgtacg caaattcttc tattatcatc gtatcgctag ttatttgttt aggaatgtct
     1921 tacgtagcgg atcttgttgg gcttagctct gtcattggtg ctttttttgc tgggatagct
     1981 gtcagtcaga caaaggttaa agaagaagta taccacaatg tagaggcttt aggatatgca
     2041 gtatttatac cagtattttt tgtcagtgta ggattagaag ttgatttttc tgactttagt
     2101 gaacaactac tctttatttc tatattgacg cttgtagcga ttttgaccaa attgcttgga
     2161 ggttattttg gtgctcagat tgccggtttt tctcaacata gttcattgat ggtcggtgct
     2221 ggtatgattt cgcgtggaga aatggctttg atcattttac aaattggaca gcaaagtcaa
     2281 ttgatcgaac cacagtacta ttcaccttta gttatcgtga ttttattaag tacacttatt
     2341 tctccacttg ttctcaaata ttttacaaaa aaagtttatg aagtgtgagt aaatcaaaaa
     2401 gaagttgtga aagagacatc gtgaaaaccg tttatttgaa aaaaggactg tgacgataac
     2461 gccacagtcc ttttactgta cttaaaaagc aattttttat tgataatttt ggttgaggta
     2521 ttgcaatagt tccgatgctc gtttggttgc tgaatcatca tcatctttat tttccaacat
     2581 gctgaccatc atataacctt ggttatcaag attgaaagca aacaagaaac tgttctcttg
     2641 cccctttaca tcctgttttt ctttgatttc cgctgtacca gttttcgctg ccaacggaat
     2701 ccctaaagca gaaagagaat gtgctgtacc atttacatct tgcacaactt ctctcagatc
     2761 tggcacgatc gtttgtactg ctgtttcgct gatgacattc ttcttatctt ttgtctcttt
     2821 atctgcaatc aatttaggat agacaagtgt gccattgttg gtaaaaacag aatacattgc
     2881 tgcttgctgg ataggagtaa ttagaagttc gccctgtcca tatccagtat cagctagcaa
     2941 gatatctgag ttaaagctat cttcattaga aatttgtgct ggattcatac taattggcaa
     3001 atcaaggtct tcaccaaaaa tgaatttatc taagcctgta cgaaattttt tctcacccat
     3061 tttcaacgtt tcttgtgccg catatatatt atcggaatag atcaaagcag tttttaagtc
     3121 tacttgtgat acatcactac tgacacgcgt tgttttaaaa gatccccaag aactgtcttt
     3181 ttgccattta agcccgttga tcgtcaacac ttcatttgga tcgatagtgc cgttgtcgag
     3241 accgattgct gctgtaatta ttttaaacgt agatccagga gcataacctg tcgcaaatcg
     3301 gctgatgaat ggatgttcag gattttcttc ataagctttg taatcttctt gtgagatccc
     3361 gtttgtcatt ttgtttggat catagcttgg agagctagca agcgcaagaa gatcaccggt
     3421 ttttggcgtt gtcgcaacag ttgatccagc ttttcctcct agactgtcaa aagctgtttt
     3481 ttgtgccttc gcatcgattg tcaatttgat atctttgcca ttttgaactt catgttcgat
     3541 cagaactttt ttctctacac cgtctgcatc tgtaatgctt aatttctcac ctgttgtccc
     3601 acgaagatcc ttatcaaaag ccatctccaa acctgagcga ccgatttttc cattactgct
     3661 taattctgga ttcttatcaa tatcttctgc tgtgatatct cccacgtaac caatcagctg
     3721 agcagctgct tcacccaaag gataatatct gccgtctact tcttgaatgg tagctccagc
     3781 tggaagttct ggcgttgctc catcaatgat tttcaatggg acaaagtaat cgggttgtac
     3841 ccagctttgt gaaatcgcct gattgatagc atcttctgtt aagtcgaagg aggaagcaat
     3901 cgctttgata ttggctgttt tttcgcctcc atctccaagt ttacttggaa cgactcctaa
     3961 ttgttttagt ttgcctgttg ttgctaatgg ttcaccattt cgatctataa tattccctct
     4021 tgctgcttct tgtgtggtca ggcttacttt gtcatttcct tccatttctg gaaaaaccaa
     4081 gttgggttgc cagttgatcg tggtttgacc atcatttctg tctaatgttc ctttataaga
     4141 aagatctttc aattcaccta atgaggtatt catctttgct ttatatgaaa aactgtaagt
     4201 agaatcgtct tttttatcta cttttagatt cgatatctga agtcctttga catcagcagc
     4261 accgtatata ttctggtatt gatctaagat ctctttttca gataatgcac ttttatttgc
     4321 cgcctttttt gatgtcattt ctgcagcttt gttataatct cccttgttca aagcttggac
     4381 aaattgctca actgtctttt ctccagcttc tactgcttgg gtttcttgat agtgctggta
     4441 gtaaaaataa ccgccacccg cagctgctat taagatcact gcgccggcaa tataagcgcc
     4501 tgttcgattt ttgccgtgct tgtcacttct tttcattttt tcctccttct cttgcttgtc
     4561 attcctttat ttcactattt atacctgttt attctaacat atgtctttga ggaaatccat
     4621 accacaatcc agaagcacac tttctggaag gttcgtttgg atactaccaa gtaatttccc
     4681 aaccgctttg ggtagcttga atattgaagt tacgtctttc agctgctgag agatagcttt
     4741 gatcacttgc tgctgccttc tgatcctccc gaagtctcct tctttgtctt cccgaaatcg
     4801 agcatactgg agtaaagtat taccatccat cacttgctct cctttgttta tatatacgcc
     4861 atctaaatct agagattttt ctgcatcgat cttgactcca ccaggaaaca gttcattaac
     4921 gcaatcgata aaatcttgaa atgtgatact tgcataatag ggagcttcaa atttgaaatt
     4981 ttcctttaat gtttgattta ataaatcaac tcccccataa gagtaagctg catttatctt
     5041 attcattccg taacctggaa tatcgacata tgaatctctc atgatagaga tcagttttgc
     5101 ttgttgtgtt ttctggttgt attgtgcaac cagaagtgaa tccgagcgac ctccaccaat
     5161 cgatcttttt tcaccgcatc tcctcctcac acagcaatcc acttgtcagg tgaggagcga
     5221 gtttgtctca tcaaataagt cagtcacaaa aatataaaga aaaggtcaga aaccttttcg
     5281 aacaagaatt ctgacctagc atcatctcat cgtccgagtt ttagcactat acaacgtaaa
     5341 gaaaacattc ttagctattg gaaaaagcct taatccaaca gtttctgttt tacccattgg
     5401 cgactttcga cgtttctggg caacagccta tgtttgtata ggagcctcac ctaacaaggt
     5461 tattactata ttctatttac gagttcatga tagacttttc ctaatgatta ggcaatggaa
     5521 tttgaaatga aatagtgaaa tttaacaatc tttaatattt tttagaaaaa aataacaaat
     5581 aagttgaaag aatcccgttg caaattcttt cagttgcacc aggattcttt gataattttt
     5641 tagttttatg tgttttttta tatgtttttt cacttttcgg aataaatatt tatttgttaa
     5701 agtaattaaa caatccgcta atcgtttgat ctggcatttc tatttcctta ccatctaaag
     5761 taacagtagt aacgacaaaa tggtcttgca atggcggaac ttctaagtac tgcccttgca
     5821 gaaaatcatt cactgtttca tattcttttt tcatttcttt tgaatctgtc gatggtttat
     5881 agcttatttt taacataact agtgctcctt tttattcggg acgtgcaatc gtgaaaattt
     5941 ctcctattgc tgcataatca tttcctgcca aacggcttac agcattcaat tttcttggat
     6001 cgatccttcc tgtcttatca tcatagactg tttcatttaa atgatatttc tgtacttcta
     6061 gcaaaaataa atcagctgtt actttatttt tattccgtat ctcgacatgt tcatataagg
     6121 atgtttcaaa acgaactttt gcttctgtca atgctggtac tgaaatgatt actgaatcag
     6181 ttaaagtcaa gtcagtcagg tacagttcgc tttcctcatt aggcagattg gctgctgttt
     6241 tatttacttc ttctacgttg tcctcatcaa ggatatggat aacagcttct cttgtctcaa
     6301 gaatattctt tgctgtgtct tttactgcat cttgttttct ctggatcgcc aaggataaga
     6361 tgggtcgatt cgaactgacg atattgaagt aactgaaggg tgcgatattc actatatctt
     6421 ttgctgattt agtcgttact actgctactg gtctagggat gactgaacca atcagcaact
     6481 tgtaattttc acgttctgtc aattctgaag gactccgttc aaacatggtt tcgccttctt
     6541 tctataaaat tcgttaaaaa gcagatcttg aagacaaaat gaaaagtaat gcttttttca
     6601 tcttcttaga atctgcttta aactatctat caatttttct tttttgtcaa gaatctgtgc
     6661 cgtgcgtgtt ttttagttat ttgctttact tgcggcttcg acatatcttt tagtgatcaa
     6721 ttgcggacgg gtgataatag atccaactac gactgaaaaa gcacctaatt catatgcttt
     6781 ctctacttgt tcaggagagt cagtatgtcc ttccataatt actgggatat cgacttgttc
     6841 tatgatttga ttcaataaat caaaatcagg tgtctgcgtc tttcgtgaat attctgtata
     6901 gcctgctaaa gtcgtagaca aaagatcaaa accatcttct tgcgcttgaa gcgcttcctc
     6961 aaaagtcgaa atatctgcca tagctaaccg accgtgttcg tgaatcattt ggacaagttc
     7021 agcagttgtt ttttctgtcg gacgtttacg agaagttgca tccagtgcaa tgatttctgc
     7081 ttgcgtctcc aaaagttctt ccacttcttt ttctgtcgca gtaataaaca cttctgaccc
     7141 atcgtaatct cttttgataa tccctattac cggtaaatca acgattcgct tgatttcatt
     7201 gatatcttct gtcgtatttg ctcgaatacc aactgctccg ccttcttttg cagccaaagc
     7261 cattctcccc ataataaaag aactgtgtag cggctcgttt tccaaagctt gacaagaaac
     7321 gacgactccg cctttgattt gttccagtgt atccacaata tctcctccag taatgttata
     7381 taaaaataca gcttcatata aaaatgtaac ccttttctat tcaggagaca ataatgagct
     7441 atttctttca gcagaccttt cttactaaaa gaaaattact tcaaattctt cgtatacgac
     7501 cagcttctct tctgtaaatt ctatgttcaa cgcttcttta taagcatgtt taaaaaactc
     7561 agcatggact ttccgccaat aagctaatgt ccgatcccct tctccttctt tccaagcatg
     7621 ctctttcgtg acttcactaa aaggcaagat ggtcacattt atatttttga tgatacaaac
     7681 tggttcattt tttccattca ggaccacgtc taattgacca acttttggta aaggctcatt
     7741 ctcttgttta tacagatcgt aaccacttga tgtaccagtc tttatctttt ttacaactag
     7801 ttcgctcaac tcgtctgcca ttttttctgt atccccaaat gaaaagaaac ctaatttttc
     7861 tgattgataa ctaggatatt tttcttggaa ctttttgaca tattcttcta cttgctgata
     7921 cataacatcc acccctttgc tttttataat gaaaaaacca tccagatact agtataaaca
     7981 attatctgga tggtgagcat aatgaattat tgaatattca agacacgcag actatcttca
     8041 acgatttgtt ttggtgttaa atgataacgt tcataaagct gccctacagg aacgctatcc
     8101 gtgaattctt tctctgcacc aaaatttaat acgttcattg aagaatcacc ataataacga
     8161 ctgatatttt cgccaaatcc tcctgacaaa ctaccatctt ctaatgtgat gactaattcg
     8221 tgtgtttctt ttaatttatc taatgtttct acgtcatttt ctgtgatcgt tcctggattg
     8281 atcaacgttg cttcgattcc tttttttcta aaagctttta ctgtttcttt tcctaattca
     8341 agaaaaccgc ctaaagctaa aattgctact tttcctccat tatttactgt ttgataggct
     8401 ggaatactat aatcatttgc gatagctgta ccatgttcta caccatgtgt aggaattcgg
     8461 attgctacag gctgcgctgt ttgttcgaac gcccaatcta gcatagagat catatcctct
     8521 tttgttgctg gagcaagata tttgatgttc gggatacttg ataagtaagg aatgtcaaat
     8581 gttccttggt gtgttcggtc actagctgag atcgttccgc caccgactaa aataatcact
     8641 ggattctcgt tgatcgctaa atcatgtgat aattgatcat atgcccgttg caagaaagta
     8701 cttgattgga agacgactgg acgtactccc tgagaagcca aagcagaagc gaaagtgata
     8761 gagtgctgtt cagcgatccc cacgtcaaaa taatgatctg gatgtttttc ttggaatgtt
     8821 ttcagtccaa aggcgcctgg tatcgctgca ttgactgcgg caataggagc tgtacttgtt
     8881 tgcattttct tatccaaata atccatgatg atcatattaa atgtttctcc agaagttact
     8941 tgctttgcct ccccggtgga cagatcaaat ggtacatgcc aatgatattt cattttatta
     9001 tcgattgccg gctgataacc gcgacctttt tccgtatgga cgtgaagtac aatcggatga
     9061 tcaatatctt ttacttcttc aaataaacgg atcaccgttt ccagatcgtt tccattatcc
     9121 acgtaacgat aatcaaatcc cattgcttta aataggttat tgctgctttt accgttggtt
     9181 tgacgaagct cagctaagtt tttatataaa ctgccatgat tctcatcaat agacatctca
     9241 ttatcgttta ctaatacgat tagattggat tgtaaaacac caccattgtt caatccttca
     9301 aaagctaagc caccacttag ggagccgtca ccgataaatg ctaccacatt tcctgattct
     9361 ttcttcagat ctctcgcttt agctaaacct gttgctaagc tgatagatgt agatgtgtgc
     9421 cccactgtaa aatagtcatg tttactttca tgttggttgg aatatccgct cacttcacgg
     9481 taatgtcctt tttcaaaacc attttttcga ccagttaaaa gcttatgacc ataagactga
     9541 tgggaaacat cccaaacgat tttatccttt ggtgaatcaa atacataatg aaacgcaatc
     9601 gttaattcta ctacacctag attcggacca acgtgcccac ctacatcgct gtccttttgc
     9661 aagatttttt ggcgcatctc ttctgccaac tgtttcattt cctcaacaga taattttttg
     9721 acatctgcgg ggctattgat tgcatccaat acatgtaata ctgtactcat aatacattct
     9781 ctcctccgta ctatcttctt ttaatgaaaa tagatttgtt acactttatc tattctacta
     9841 cttagagttg actctaagtc aatagcattt taaaaaaatt tcagagaaag cttgttatat
     9901 aagtagatct tcgcacgaat caggactcaa ataaaaacct tctacagaca attaaaaagc
     9961 tttgtagaag gtctcctaat taaggtctca tcaagaaata tgttttttta ataggtgaag
    10021 ttcctgagca atatgataca aatggatgtt tgcacctttt aatgagattg gatagatctt
    10081 tgtcttttca gtttctaatc cattttgata gctagcagca tcgcacaaaa ccactctttt
    10141 tttcgcacat agacattcat caggcaattc ttctaatgaa cgaaataaaa taggatttat
    10201 ttcagcgatg ccttcttccg catacaaatg aaattgggtt cgataaaaaa attgtttgac
    10261 tgaactttct gaaacaacaa aaatttctat ttcctgtctc cccgcttgtt cactgttgat
    10321 caaagaaaga cttttgacga attttcgaat gaaattttca ttccatctta gttggatttg
    10381 atttttcttt ttccactctt ccaatatttc tacaattttt ccgtacagaa gttgttcttt
    10441 ttctgataga agatagatcc tttccggtaa aaatacttgt ccatctgccc acaacgtacg
    10501 gacaaattgt aaaaaggatt gcttgaaaat aaaattttct gcatcgtcga tttcagcgat
    10561 actaataatc tgtaaaatca attcattcag tgataggtca ttgagaacaa aatttttata
    10621 aacaacttcg aaatcttttc ttaacagctc catgttctca ttggtataat ttctactgtt
    10681 gaacaaagaa aaaatataat agacatcttc ttcttgtaag tctagaattc ctttttgctc
    10741 ttccaatcct tgacagacta attgaaataa cggcttcgtt tctgcgcttt tcttatcttc
    10801 ttctttcagt ccaaggggaa atgcctttct tccgattgcg atctctattc ctcgtatgat
    10861 aaaggacttt tcatcttccg aatatcttct tttaaaaaaa tgagtctcta cataatcaat
    10921 caactcgtta caagcttctt ctacatacgg atttttattt tcatagcctt cccaattgac
    10981 ataacgtact aaagctagca agatgttccg cgagtcaaat tctgggataa tgatttcttt
    11041 atttttcaaa taatcatttt ctttaaaaaa atccaatacg atttttttga ttgtatatac
    11101 tttgctcaaa gaaataaatt caagatcagc cagttctgta ctcgtgaata taccattaaa
    11161 ataataactt aaaaacttta aaaaataaga ttgaccgtac agaaactgtg tgagctcaga
    11221 aagagcaata ccatgtttga atacgagttt atacttatgc ttctctctta cttgttcttt
    11281 tatacaatat tctagacact cggctagact ttctagatcg tgaaggatcg ttggtgtagt
    11341 cacccttaga gaaatagctg tttgttcgat gtctatttct tggaattcaa ataattgttc
    11401 acacagatac acttttctaa atatatttcg ctcgatgtaa ttttccagca ttcctgatac
    11461 acgcctccag tttttcttat gattttattt aatcatatct gtaaatcgta tttttgtttc
    11521 ctttttattt agaaaagccc tttcaaaaac caataaaaaa agaaactggg acaaaagtca
    11581 aacaagctca caatttcagc ctcattgtag gtagctttga gccgtttgga cttttacccc
    11641 agcctctttc aagaatggtt attcaaagat gtcagcgtct agttctttga ctacatcttt
    11701 caacaattgg ataccagctg cgctgttacc aaatggatct agtgccgggc tgaagacacc
    11761 tatgccgtaa cgatttggca cagctgccat caacccgccg cctacaccgc tttttgcagg
    11821 tacaccaaca tggacggaaa actctcctga ttcatcatac aaaccagcag ttgtcataat
    11881 tgatttgacg attgtagcac tttcttcagt aatgatttgt ttgccattcc aaggagcaat
    11941 tcctttattt gccagcacag cagctaattt tgctatgcct gtagcggtga cattgaggga
    12001 gcattgtctg aaataagcat cgagtatctc cggtacgtct ccttcgatca tttgattacc
    12061 tttcatatag taagcaagag agcgattgat atcacctgtt cttgattcag aatgatagat
    12121 ttcttcattc aatgtgatat taggatcatc acagatctct ttcataaatg acaagattcg
    12181 ctcaaatttt tcatctgcat ctttaccggc aatcaacgag gttgttgcaa tcgctcctgc
    12241 attaacaaaa ggattcattg gatgtttgcg atgattgatt tccatattca tgatagaatt
    12301 gaaggcaaaa ccggttggtt ccgtgttgat tgtttgaaag actttttcta ttccgttgtc
    12361 tttgattgct aataacaata cgggaacttt tgacatgctt tcgatggcaa agcgcacatc
    12421 ggcgtctcct ccctcaatca gttgttttgt atccaaatca taaatcgcca tacctaattg
    12481 tttgggatcc acttcagcta atgctggaat gtaatccgcc acttgtcctg tttgataata
    12541 gtggaaattt tttccaatca attgttctaa ttcttttact tccatttgaa taaccagctt
    12601 tcatttttta tttttctgca tgctggaatt tatgacggca tgcgtagatc gtaaatggaa
    12661 taactaagac gataagataa gaaacaatca gaatcgtttc ataaatccca tgctgtccag
    12721 ctgcgatact agcaggtgga acaaatgaga tacaaaaagc aaaaagtgaa gtcaaaagac
    12781 ctaaacaagc aacgattact tttacaactt ttccgcctgg aacctgatag gctctttcaa
    12841 gactgtcatc tttaaaaacc agcttcaaat agcccgcgaa aaacataaag tacaacatca
    12901 ggtagattac aacagtcaat gacatcgctg caagaaatga aagattattc cccccgccgc
    12961 ttaatgtaaa gacggctgcc cagattgttg caacgatccc atttgcgatc atcaaatgag
    13021 caggaacacc gtgtgtgttt tcttttgcaa aatattcagg aagtaatcct tcttctgctg
    13081 ctacgtgaag tgcttttgaa ggagaggtta cccaagaagc aatttctccg atgacaccta
    13141 aacaaacaag aatcgcaaga attttcgcaa tccatgcaag atttggatca atgtgtctta
    13201 ataggaattc taacgattga atcacccctg tattaagaga caaaccatgt tgaggtactg
    13261 tagtagcgac ggtcaatccg ccaaaagtat ctaaaatgat tgccagaata accaatacaa
    13321 acattgctaa tggataatcc tttttagggt tttccatttc atttgcatga ctggcagatg
    13381 tttctacgcc catatatgct aaaataaagg aaacaaaaac aaccagcgta ggcaatttcg
    13441 aaaaatcagg aatcaatgtt ttcatagaca gtatcgtttg tagtggagcg cctgaagcga
    13501 cataatggat ccctaagcca aacaagacta atgctggcaa caagatacca gctacaaaac
    13561 cgatccgagc aatcaatgaa gtgtatttgg ttccttttaa ctgtgagagt aaaatagccc
    13621 agaaaataat caataggatt gccaatttca gatatttatt gttttgtata gcaccaatac
    13681 ccgttgcata ggataatgca ccagttaaga aatacagcat ggtcaagtag ccaactgtta
    13741 tctcaaacca ttggaaaaag attgcggcaa aaccgaatcg ttttcccaat gtgcgactga
    13801 cccaagtata gacaccgcct ttttcccatc ctttcaccgt tgccatctct gctgcgcaca
    13861 atgccacagg gataaaccat agcaaaccgc ctaataacaa gaaaaatact aagctgaagc
    13921 ctgaggtcgc gaatgtggga tattcatata atgaaacgac cattgagatg gtcatagcca
    13981 aaaagccaaa gaacgttaat ttttttgcag gaattttcgt tttgctttgt tgcatgaatc
    14041 ttcctctttt ctatttgaat ttgtttcaat tgagtttgtt gccaaagaag aaaaagtcca
    14101 atccttcttc ttgttcttct tttgtagtag tgatcgttgt aaagtgcatc gccgatttta
    14161 aaatcactgt tgtcagcaca taggcataca cggcagtaat gatgacacca atcacttgaa
    14221 tccctaagaa gtaccagctg cttggattcg ttagtaaatg gctgttagat gcaaagatcc
    14281 ctatcagaat cgtacctgtg aatccgccca tgccatgaac accccacaca tcaagtgcat
    14341 catcccattg tttcttttta cgaaaatcaa cagcagcatg acagacgata cccgcaatga
    14401 tcccaatcag catggcactt tttgcttcta cataaccggc acaaggcgta atggctgcca
    14461 atccagcaac ggatcctgtt agtacgtcga caaaatcaaa gccatttcct tttacttttg
    14521 caatgatcag ccaagtaatc atcgcaaatg caagagcgat gaatgttgaa ccaaatgcat
    14581 tgactgcttg ttcatctgct cgaagtgcac caccggaatt aaaaccgaac cagccaaacc
    14641 aaagcagtcc tgtaccgata gcagctgcca taagactgct atgctgactt ctttctccta
    14701 aaggaatctt tctttgacca agtgtgagga tacatgctaa agagccgaat ccagctgtcg
    14761 tgtggatgac tgctccacca gcgaaatctc taaatcccat ctgatccaaa aatccgccac
    14821 cccatatcca gtggcaaaca gggaaataaa tcagcagatt ccaaaaaata acaagtataa
    14881 tatagccttt taaattcaat ctccctgcaa aagctcctgt catcaaagga acggtaatca
    14941 cgataaacat cagttggtaa agaaagaaca ttaaaaatgg aatcgttgca ccatgaaaca
    15001 tgttggggaa gcttgtaaca tgtctcatca aaaagaaatc tgccgggttt ccaataacac
    15061 cgccgatatc ccgtccaaag gccaatccga atccaccaaa tacccacata atcgttgtta
    15121 tcccaacgga gagaaaagat tgagacatga tcgttagtat attattcttc cgtgctaatc
    15181 cgccgtaaaa aaaagctaga ccaggagtca tcaggcaaac cattgctgta cataaaatca
    15241 tgaatgctgt atctcctgta ttgataggta atgtattcat ttttttcgcc tcctttctac
    15301 gattacgagt ataaaagaag gtgtagttgt ttttttcttc tgtttttttg aattaatact
    15361 tgcatttata aaagacagac aattaataaa agcttgatgt gttgtgctct ttttcgtttt
    15421 tcacacaaat aaagacaaaa aa
//
LOCUS       DANJMI010000060        14960 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000060 DANJMI010000000
VERSION     DANJMI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.060"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            4..2376
                     /locus_tag="PWU81_002412"
     CDS             4..2376
                     /locus_tag="PWU81_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111081.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="PWU81_002413"
     CDS             2437..3897
                     /locus_tag="PWU81_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDL1111082.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="PWU81_002414"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="PWU81_002414"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL1111083.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="PWU81_002415"
     CDS             6128..6277
                     /locus_tag="PWU81_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111084.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6352..6945
                     /locus_tag="PWU81_002416"
     CDS             6352..6945
                     /locus_tag="PWU81_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDL1111085.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6958..7857
                     /locus_tag="PWU81_002417"
     CDS             6958..7857
                     /locus_tag="PWU81_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDL1111086.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7860..8357
                     /gene="ssb"
                     /locus_tag="PWU81_002418"
     CDS             7860..8357
                     /gene="ssb"
                     /locus_tag="PWU81_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL1111087.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8723..8983
                     /locus_tag="PWU81_002419"
     CDS             8723..8983
                     /locus_tag="PWU81_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111088.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9428..9634
                     /locus_tag="PWU81_002420"
     CDS             9428..9634
                     /locus_tag="PWU81_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDL1111089.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9682..9885
                     /locus_tag="PWU81_002421"
     CDS             9682..9885
                     /locus_tag="PWU81_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111090.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9901..10338
                     /locus_tag="PWU81_002422"
     CDS             9901..10338
                     /locus_tag="PWU81_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111091.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10331..11038
                     /locus_tag="PWU81_002423"
     CDS             10331..11038
                     /locus_tag="PWU81_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111092.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11418..11597
                     /locus_tag="PWU81_002424"
     CDS             11418..11597
                     /locus_tag="PWU81_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111093.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11922..12185
                     /locus_tag="PWU81_002425"
     CDS             11922..12185
                     /locus_tag="PWU81_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HDL1111094.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12179..12529
                     /locus_tag="PWU81_002426"
     CDS             12179..12529
                     /locus_tag="PWU81_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL1111095.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12553..13167)
                     /locus_tag="PWU81_002427"
     CDS             complement(12553..13167)
                     /locus_tag="PWU81_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1111096.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13481..13903
                     /locus_tag="PWU81_002428"
     CDS             13481..13903
                     /locus_tag="PWU81_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111097.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13913..14116
                     /locus_tag="PWU81_002429"
     CDS             13913..14116
                     /locus_tag="PWU81_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111098.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14327..14911
                     /locus_tag="PWU81_002430"
     CDS             14327..14911
                     /locus_tag="PWU81_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1111099.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5512 a         2429 c         3034 g         3985 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac
      241 gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgg ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctaga
      661 ccaactgatt ctatgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacattgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaggaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaaccaat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaagggt tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc
     4981 tattcataca ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat
     5041 cattcctaca aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca
     5101 agcgatttac gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt
     5221 accgatagat ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc
     5581 tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca
     6301 ctatcatgat atatttaatg tagtgtattt gtaacaaaag gggggaagta aatgcaagag
     6361 aaactgaaag aattgttggc tattcgcgat ggaaatttat ctatgaagga agccagaaaa
     6421 ttaggaatcg cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt
     6481 gatcgtggtt attatgtaga ggatggtcat ggaatagatg atttattttg gcttcagcaa
     6541 cgtttttcca ggggaatctt ctctcatgaa acgactttag atatttacca gctttctacg
     6601 aatatgccga aattcattca cttaactttt ccccatggat ataacgttga tcgttcaatt
     6661 acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa
     6721 gtagagggaa cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt
     6781 tgtgatattt ggaatccgag atatgaaatt gaatatgaaa tgaaattaga agcattaaaa
     6841 gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta
     6901 catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg
     6961 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca
     7021 cagttagtac aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag
     7081 tataaaaatg atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat
     7141 agaaggacga caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa
     7201 ttgaagagta tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct
     7261 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
     7321 gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct
     7381 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaggtggag
     7441 attccagctt atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt
     7501 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
     7561 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
     7621 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat
     7681 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
     7741 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
     7801 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     7861 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     7921 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     7981 acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa
     8041 tggtgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga
     8101 cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc
     8161 aactattaga aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca
     8221 gtccaagcac caccactttt gaacaacggg atacagcgac gcctaataac aatggattga
     8281 acgctttaca gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg
     8341 atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata
     8401 atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt
     8461 ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca
     8521 aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa
     8581 acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg
     8641 tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta
     8701 taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt
     8761 aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa
     8821 ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg atggaaaata
     8881 caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta
     8941 tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa
     9001 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc
     9061 tttttaggaa ggcgcgagcg ccttctcaat tgaaaaacct ctctcactcc aaccgccacc
     9121 gccgttcgag aagaaaggaa gtgaacgttg cacgttcact aagataaagg gtcttgcaaa
     9181 gcaagacaaa atagaaaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct
     9241 ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa
     9301 gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca
     9361 acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga
     9421 atcaatcatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa
     9481 ctatcatgca ccacatactg tcgaatgcga ttgtggacaa agagcgaaat gtatcaaaag
     9541 ctatcgtttg tatcaatgtc caacatgcgg aaaaaaatac accttgtctt atggcgatta
     9601 tattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cataacataa attattgact
     9661 gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa
     9721 gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact
     9781 atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg
     9841 gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat
     9901 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
     9961 atggaggata tgacattcct ataccatgag acacgtgttc caaaaaaaca ttataaggaa
    10021 agattgtcgg tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
    10081 acgtactatc gaacactgga agaactgaaa aaacaaaatg caaaatggtt tttccaagcc
    10141 ttactatgct tggaagtagg tgtcaaacca agcactatta aaccttctga atatcaagct
    10201 ttagaactaa cgtatgggaa atttgtcgaa acgaaaaaag ccaagactgt ttcaagtgaa
    10261 tggttagatt attttgaaaa tatcaataag tatggcgcct attacaccat gaaaaaggag
    10321 gacaacgaaa atgaataagg tgatttatca actaacaacg gaagatgtgt cacacttttt
    10381 aacaaacaaa gaacatttga atgaagtaat cagacggatt caaaaagact ttagttgtat
    10441 ggaagaaatg aagtacttta ttcaaagagt cgaagaacaa ttgtatttgg aaagtgaaac
    10501 gagtcaaacg aaggtcacag tcagcaataa ttatttagac gtgtataaac atcgtcctga
    10561 aacggtaaaa aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt
    10621 ttacctagaa aggagtgaac tggtcgaaaa ctttgtcagc tgcacgattt gcaggaaaca
    10681 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    10741 aatggaagag ctaaacagca aaaatgcgac gatagacgat tattatcttg tatttactag
    10801 atttttgacg tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt
    10861 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
    10921 cacgacttta actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa
    10981 tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaaa ggcaataatc
    11041 atttataccg aaaagtgaga atgtgtgaaa taagaacaaa aaccttgaaa ttaagggaat
    11101 ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag
    11161 cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc
    11221 tgttgttcgt actgtgtatt ggcgagatac ttatgcgtca tggtgcagct gtacatttaa
    11281 acttcgtagt attcttagga atactagtat tttgtttgat tttttggcgc tttgtgttag
    11341 cccttgtgag tctactattc aagttgattg cagccatatt tttcataggg ttatttcgat
    11401 gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc gtgacattag
    11461 atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa agtgaatcta
    11521 agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga tttgatatac
    11581 cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata gcacttaatt
    11641 gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat caataactat
    11701 gaaatcttta aagtatccag atcaattttt ttcttacctg attctaaaga ttttgttttc
    11761 ttaaacatat tatgatttcc tttcgactat tttcatataa atgttaacat agtttatgga
    11821 ttcatggaat gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt
    11881 atgtatgtta tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa
    11941 aaaaatgggg aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa
    12001 ttcatacaga agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa
    12061 ttatcgaagc ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca
    12121 ctgatgaaag aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat
    12181 ggtgagaaaa cctagacaag gcgatattct attattgaat acagcacctc gatctggaca
    12241 cgaacagaca ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag
    12301 taacgttgtt acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa
    12361 tataaactca gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac
    12421 aattgattat gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga
    12481 ggagttattg atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag
    12541 cagcatgatc aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg
    12601 tctcgtaaca ttgtagtctt ttgcaatctt agcgatcgca acaccttttt ttagatcctc
    12661 aacgatgttt ttataaacaa gacgacgttg gggatctttt gcattggcac tgtatagctt
    12721 aggacgtcct ttataaacac cgttcgcttt tgcaatctta atgccttgtg cctgacgtcg
    12781 tttagattcg gtacgttctt gttcagcgat catggccaaa atttgaatga tcaaatcttt
    12841 aatgaattta tccaggagag gattgccaat tgcttcggcc atgatcggta gacttgtgat
    12901 gatcagctgc acatttttct tcttcaagta attgactgaa gcgatgatct cgtcgtaatt
    12961 tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga cgaaatccag
    13021 tgcttcttga aaaacaggtc gatgagtaat cgttgcgcca gattgtttct caatgaaaat
    13081 tctctctgca ccgaattttt tcatttcttc aatctgccgt tgttcatttt gatcgatgga
    13141 tgagacacgt atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaaa
    13201 acaccctaat catacacgag aaaaccagat ttttcaatgt cgcggtcgtg tctagttagg
    13261 gtatacctta tatagacaaa aactaatgat ggaggaaata gtatgtgtca cttttctttt
    13321 taaatggcaa tattttttgt aaataaatgt caatcaagaa aatttaaacg attatgacat
    13381 taaaaatata ttttatgaat tttctagtat ataacttgat ttatctgtta agatttatta
    13441 agaaaacgtt ttcttatttt tgatttcgag gtgatagaca atgaaaaata agtttatttt
    13501 atctttaggt attttaacta ttggaattag tgtaactgga actacaattc ctacatttgc
    13561 tgatagtaca aatgaaccaa ctactgtagt agataataac caagaattaa ctcctttaga
    13621 taggaacgga atagccattg gtaacccaac taacgactta actgttactg aaaattattt
    13681 aatagtttct ccattacttg ctcgagcagc ttctaaaacg atacgagtga caggaacgat
    13741 gatttttgat aaagttggtt ggccttctag acctcctcaa tcacggaatt ggaaagaaaa
    13801 aagaaatggt tattggtact atggaaaatt gtatttacaa agttataaat cagctggaaa
    13861 caattggatt gcgacttatt caggaacctt aactagaaag tagggatttt ttatgccaga
    13921 atttgtgaac acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa ttaatttacc
    13981 gaaagaagaa tcagctttac aagttcagcg aactttacag gagacacaaa aaattcaaaa
    14041 atcaatttct aatctagaag attctgaaga aaaaagaaaa aaaagagacc aaaaaacata
    14101 tcaaaaagaa aaataatgat tttaaaaaaa gagtgtctag tcgacattct ttttttctaa
    14161 ttgaaagtgt tcataaaatc acccatttct ggacactttc tttttttgct ttatttttca
    14221 aaaaaacaat tcatttcatg ttcataaacc cataacaata tctatgttca gttaaactat
    14281 atggataaag ttatgttaca ataaaattaa tgaaggagtg atttttttga aaataggata
    14341 cgcccgggta tctactggtt tacaaaattt ggatttacaa gaagacagcc taaataaatt
    14401 tggttgtgaa aaaattttta ctgatcatat gagtggggca aaaagtaatc gaccaggatt
    14461 agaaatggcc attgattttg ttcgctctgg cgatacactt gtcgtttggc gcttagatcg
    14521 tttaggtaga aatatggaag atttgatttc aatcgtcaat cgactgaatg agcgtggtgt
    14581 gagttttcat agtattcaag aaaatattac gatggacaaa tcaagttcta caggacagct
    14641 catgtttcat ttgtttgcgg catttgcaga atttgagcgt aatttgatcc tggaacgatc
    14701 cgcagcagga cgagcagctg ctcgtgcaag aggacggttt ggtggtcgtc ctgaaaaatt
    14761 gtctgaacaa gatttggaat tactgaagac tttagtggat agcggtacac ctattaagac
    14821 tattgcggaa cggtggaacg tctcaagaac caccatttat cgttatttgg caaaaatgga
    14881 ggagaaaaat ggaaagacaa attctcatta gtttttgtca tttaatgctg aaaatttttg
    14941 ccatttatct agaaaagtga
//
LOCUS       DANJMI010000061        14820 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000061 DANJMI010000000
VERSION     DANJMI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.061"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(296..1024)
                     /locus_tag="PWU81_002431"
     CDS             complement(296..1024)
                     /locus_tag="PWU81_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HDL1111100.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="PWU81_002432"
     CDS             complement(1002..2576)
                     /locus_tag="PWU81_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL1111101.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="PWU81_002433"
     CDS             2805..3743
                     /locus_tag="PWU81_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1111102.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="PWU81_002434"
     CDS             3755..4666
                     /locus_tag="PWU81_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1111103.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="PWU81_002435"
     CDS             4690..6162
                     /locus_tag="PWU81_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL1111104.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="PWU81_002436"
     CDS             6213..6824
                     /locus_tag="PWU81_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL1111105.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="PWU81_002437"
     CDS             6854..8767
                     /locus_tag="PWU81_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HDL1111106.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="PWU81_002438"
     CDS             8895..11876
                     /locus_tag="PWU81_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HDL1111107.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="PWU81_002439"
     CDS             11873..13534
                     /locus_tag="PWU81_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HDL1111108.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="PWU81_002440"
     CDS             13574..14506
                     /locus_tag="PWU81_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HDL1111109.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4818 a         2333 c         2979 g         4690 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagttgaaa
//
LOCUS       DANJMI010000062        14690 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000062 DANJMI010000000
VERSION     DANJMI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.062"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(279..494)
                     /locus_tag="PWU81_002441"
     CDS             complement(279..494)
                     /locus_tag="PWU81_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111110.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
     gene            complement(546..1460)
                     /locus_tag="PWU81_002442"
     CDS             complement(546..1460)
                     /locus_tag="PWU81_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111111.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            complement(1460..2359)
                     /locus_tag="PWU81_002443"
     CDS             complement(1460..2359)
                     /locus_tag="PWU81_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDL1111112.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            complement(2359..2946)
                     /locus_tag="PWU81_002444"
     CDS             complement(2359..2946)
                     /locus_tag="PWU81_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111113.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            complement(3110..3301)
                     /locus_tag="PWU81_002445"
     CDS             complement(3110..3301)
                     /locus_tag="PWU81_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111114.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            complement(3374..4276)
                     /locus_tag="PWU81_002446"
     CDS             complement(3374..4276)
                     /locus_tag="PWU81_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111115.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            complement(4293..4769)
                     /locus_tag="PWU81_002447"
     CDS             complement(4293..4769)
                     /locus_tag="PWU81_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111116.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            complement(4785..5384)
                     /locus_tag="PWU81_002448"
     CDS             complement(4785..5384)
                     /locus_tag="PWU81_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDL1111117.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            complement(5388..5441)
                     /locus_tag="PWU81_002449"
                     /pseudo
     CDS             complement(5388..5441)
                     /locus_tag="PWU81_002449"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5943..6422)
                     /locus_tag="PWU81_002450"
     CDS             complement(5943..6422)
                     /locus_tag="PWU81_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111118.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            complement(6441..7070)
                     /locus_tag="PWU81_002451"
     CDS             complement(6441..7070)
                     /locus_tag="PWU81_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111119.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            complement(7067..8443)
                     /locus_tag="PWU81_002452"
     CDS             complement(7067..8443)
                     /locus_tag="PWU81_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDL1111120.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            complement(8523..9785)
                     /locus_tag="PWU81_002453"
     CDS             complement(8523..9785)
                     /locus_tag="PWU81_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111121.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            complement(9798..10145)
                     /locus_tag="PWU81_002454"
     CDS             complement(9798..10145)
                     /locus_tag="PWU81_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111122.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            complement(10165..11034)
                     /locus_tag="PWU81_002455"
     CDS             complement(10165..11034)
                     /locus_tag="PWU81_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL1111123.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            11355..12782
                     /locus_tag="PWU81_002456"
     CDS             11355..12782
                     /locus_tag="PWU81_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HDL1111124.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTYGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGY
                     KTPKFVHFLSHLKLYPILPYTAPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTIT
                     RDGYRTYKSNPKFCVSCPLLENCTLSTKHQKIVTRHIWADLMDELEHQRHTALNREIY
                     KKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKTKKPM
                     AFYFKIMQRLTKRSGLITNSFLSTI"
     gene            complement(12804..13610)
                     /locus_tag="PWU81_002457"
     CDS             complement(12804..13610)
                     /locus_tag="PWU81_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111125.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(13698..14498)
                     /locus_tag="PWU81_002458"
     CDS             complement(13698..14498)
                     /locus_tag="PWU81_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111126.1"
                     /translation="MVGVSKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
BASE COUNT         4211 a         2928 c         2002 g         5549 t
ORIGIN      
        1 catctttaaa gaaatacgat caaaaaacat aattcccata ttatttgctc gttcaacaat
       61 ttgaaatata tctattagtt cttctttttt cataactgct cttcctttaa cttgttaatc
      121 taacttgctt acaaatatat cctaccacat ttgtgtacta tctgtcaata tttttttgtg
      181 tataaatgtg tattataaaa caaataattt tacacaaaca aaaaagagcc taactgctct
      241 tagcaataag ctctcatcac tgctcctcgt gaatttagtc agtacaatgt atttgccttt
      301 tctttgataa aatccttata cgaaaaattg ttcaaccaaa attgttttag cactaccgaa
      361 tttttgtttc catcattcaa ctccaattga ccaaatctgc tgatcatatc atcaatttca
      421 tcaattatct cttgtgccat catgccgtct tctagacgtt ctaatacgta caattcaaaa
      481 acatcccttt tcattcaaaa tgcccctttc aggttacctt ttgccatttt atgggctaac
      541 tccgcctact cagctttttt tatataccta ctgatatttg ctttcgtatt tcttggcata
      601 ccacctgctt gcaggtaatg ttcacgataa taagcatcta gctctgaaac agctttttct
      661 aatctattcg ttgcattcaa cactgagcgg tattcacgtt tttcccttaa tacacgtctt
      721 aaatggttca tcgctgatat ataaggctta tagtattcca atacagtata tcctctagaa
      781 atataggcaa ttggatttct agcttcatgg atcaattcta aaatatctgt tggatcaggc
      841 ttatccggca tctcttcttc gatttccata atactgatca agtagtccaa tccggaatca
      901 tcgactccat aactttcaca ccattctttt aggttgttca agtcatgaat aacatcttca
      961 ggatcaaact ctctaaaagc agtgatccct tggctataat attttatata ttccattgcc
     1021 gattcaaaat cctctggcgg tatgaaagct tcaatttctt cttcatcaat cgctttcagc
     1081 actattccga tacctgagtt tgaaggatat tttctcaatc cttcttcaat taaatcctca
     1141 tctattcgtc ctttttgaaa catcaccaat aattgttcta acagctgatc taaaaccctg
     1201 cgctcttcat tcagttccat tgcattttta acttcttttt caccgagttc aaagtaagct
     1261 ttgatcaact tttcttgttc ttttcgttca actaatttct tcttcaagtc attcatattg
     1321 agaatgaaat ccatgtgatt actcttttgc tgattgacaa aatcatatct ttgttccaaa
     1381 acatcccgat atttttttag ttcttcttga attatttcaa agctatcaag tacatgaaga
     1441 attttttcat tattattcat cagcccatct cctgtagtct cacgtttgtt ttcgactttt
     1501 caaaagtatt gtatttaaaa gcaattaacc ctacaaccct gctatctttg atgcctattt
     1561 ccttgttaaa aattccgtcc aatgttcctt tgactaaatt atcaatatcc ggctgtgaag
     1621 tacgcatcag tgaatcaagc tttttcttat cttttctcac ttggtaaaaa taatctatct
     1681 gagctataac ccccatagga gcattcatta cctttttcag ttgatcattt accaaatctt
     1741 tctctttaag cgtattcttg atattttcca aatagtcgtt gtactctttt ggatagtaag
     1801 tgataatccc atttcctcta aatttagact ttggtcttgg cgttgctact ggtttaaaat
     1861 caaatgtagc ttcccatata tctccgttca atccttcaga agtatcccac tcaaattctg
     1921 aatatcgttt gatcctcaca actgtttttc cttcgttcgc cacattatat ttaaaagcaa
     1981 ttaagcctgt gatatggcta tctcgtatag ctgagatgtt aaaaatacca tccatagcac
     2041 tttttactaa attatctaaa tctggcgctg ttgtcttcat aatgttatag acttttttat
     2101 agctttttgg tatctggtag taaaagtcca cttccataat tgcgcccata ggatcattta
     2161 caatcgagta aaattcatca tctaataaat cttgatcttc gataaagctc tttatttttt
     2221 tcaaataatc aagatacttt ttatcatagt aggttgaaat ccttttctct gctacgtcat
     2281 tagtttcatc agcagcattc cttcttgcac ttggtcttgg tgcagataca ggttggaacg
     2341 gtaaagttac agtccacatt agtctttttt gatctctttc aaatagattt ttttaaaggt
     2401 atcaaggctt aaaggagcta ccttttgcat cttccggatt ttatttactt gttccaattg
     2461 tttcaataga ccatcttctt ttctaaggta ctcaggattt gttatttgtt tattccgata
     2521 ctcgattaat gattgaagct ctcttactgt taaaacatcc tcaatttttg cttctttact
     2581 cgttatcttt tttagatacc ctaatactgt tggataagat ttgccgatca cttctgcgat
     2641 ttctttttta ttgtactcac caaattttcc aagcaaccag tcattcatga tcttacttga
     2701 tacttttggg tatacaagaa ttgctttttc aatggaatga ctgccattat ttctgatcag
     2761 ttgactatct ttacactcaa ctgtaacgcc atcacctaaa tcaacaacat attgccaaac
     2821 gcctcttcga ttcttattaa gagaaataat cgttccctct ttatctctat aatcctttga
     2881 taattcactt actgttgtaa ctctttcccc aatgctaaaa tgtctaatat cattattgtt
     2941 ttccacttat tatccctcca atattgctgg ttatctcttc ctctgacaag gaactatata
     3001 tcatataaag aattttaaca ttattcggaa tgagtgtaaa gaaactttat ataaaaagag
     3061 tgatttagtc taagaccaaa tcactctttc ttttttatta attatattgt tatctgatag
     3121 acctattagt aaatgcacgt cttattgttt gatcattctc gatcaattct tctaacaact
     3181 ctgaaactgt tactttctta cctcttgctc tttcttcttc tttagctcgc ttttctaaat
     3241 atttaacaga agctattttt aaagtcacat actttctaac tttttcttta tttactacca
     3301 tcagctatca tctccaaggt gtcacaccta tatattattg gtgtgacacc atttgtttat
     3361 tataactttc tggctattta gccaatttaa tcgttaatgc ttcgtttgct gtagaatctc
     3421 caaaaacttt accgtcttca taaactacat gaactggcaa atctgcaccg tctgaatctg
     3481 gtttagcagt accacgatac aaaccaatta ctctttttcc tagttctctt gcaaccgtaa
     3541 cttttttatc ctgatccata gattcaactg aattgaataa ggtaacatta atcatttgct
     3601 tttcagtatc caatttaaca gttttgatag ctttgaagac ttcagtgtta aaccgatcag
     3661 ccatagaacc agaaacagat tcaaacgatt tgactttttc aggatcaaaa tcaattttat
     3721 ctggtagttc cgcttcgaca gtagaacttg attcagttgt actttcttct gtagctactt
     3781 cttttttcgc tgattcgata atctttttcg ttgatgtttt atttattttt acatctttat
     3841 agtattcttc tccatcaacg ttaaaaacca atgtcagctt gtgtacaggg ctattctctt
     3901 taactgtata aaacacttca aattgtgatt gagacggctt aatagtagcg agaacgtcgt
     3961 ctgatgcttt cttgccatct tccttcactg tcagcgaaac atttttataa ctcgttacat
     4021 tttcaaatgt tccatctatt actacccttc ccaaagaatc tggagcataa ctattattaa
     4081 ctcccgatat accaaattgt ttatcgttac ttccaaaaat cttagtggca ttaaacacaa
     4141 taaaggttac aatcacaatt ccaatgccta tgttacgcat aatcggatta tttgtttgaa
     4201 attgaccaat aattatagcg attgccccaa aaaaagcaag aattaaacat aaataaccta
     4261 acagggttaa tagcatgatt catcacaact ttctattctt tatttttctt tttggttttc
     4321 gtttgttcaa actcatgctc atttagataa gtaatcaata aatctcgcat ggatttgcta
     4381 tccttactta gcgtatcaaa cgcttcttgt aagtcagcat ccttctctac agcattaaac
     4441 caatgcttcg ttccaaattc aaaaagatta ctaatcactc ttaacggttc ttgatcagta
     4501 ttcgtattaa gccattcaag tatccgttgt tgagctaatc ctaatttact ttcgtcttcg
     4561 tgatttgtac gtgcttcata gattttagtg tgtatatcat caaaatagtg gattcgttct
     4621 cttagcgctt cacgttgttc gtacatagcc cttttatttc taacttcttc gtctattgtt
     4681 tcaatctttt ttcttaacag tatttgaagt gataacattt ctgagacaga caaaaacact
     4741 ggaattttct tttcttttct ttttggcatt tttagcactc ccttttaatt cttttgtttg
     4801 atcaatgaaa cgttctttag aataaatccc aagatcattc cacagacaaa tccgattaca
     4861 tgtgacaaga catttgcctc gcctgattca aaagtagata ccaataaaat gccgattatc
     4921 aataatacaa aaacacttag taataaatgt ttcttgctat caaaacggat gtatgcaatc
     4981 gcaaataaag caatccatcc aaaaatacct ccactaagcc ccatacttac gatgttgctg
     5041 tgtagcagaa aaggaactaa gcttcctaga aatcctgata tgattaaaac gatcaataat
     5101 gttaaccact catactgatc attcagtgct tcttcaagaa ctactaagaa caataaatta
     5161 gaaattaaat ggtacaagtt aatatgagaa acgatagaaa tgaatatatt cgggaacaag
     5221 tgtccttcat acaaaccacc taaagcaacc atttcctttg cactcaatgt atttccaatt
     5281 atgctttttc caatctgcaa aaaatcaaac aggaaaatca atacacaaac aaaaactaca
     5341 gtcgctacta ggtatcgact actatttttg tttttttctc tcattcgcta cttcctttgc
     5401 tttagtaact gccgattctc catattctct aaaaagagtc gatgttagct ttcctctcgg
     5461 aatattgtat ttttttaaaa tttcagttgt tgaaagattt ttttcaaagt cttcaataat
     5521 ttgtttatga agatttattt tagccaaatc attggttgtc aaaacaggtt ctttagcttg
     5581 aatatcgcct tgacgaatta acctttgaat tttatttttg ctttttaaaa aaggatattt
     5641 ttcaattaaa gggtcaattg cattcccttc atcaatccac ttacctattt cttgataaac
     5701 tctcttttga aattttttcc tcagctcaat cgggttcgga aaaacattgt gcttaaaatc
     5761 gtgtgatact aggctctttg atttgcctaa ttcatcagcg acttcttgca ttgtaatttt
     5821 gttttcgata tagcattgga caagagcatt atatttttta gggttattat tatttattgt
     5881 catgttctta cctacgttca ttgctaattt tttggtattt ttctgtttgt gtattgcgat
     5941 ttttaaaagg catttcctaa tacgttaaat tttgataatc ggttcaattt tactgcttta
     6001 tcaatttctg cctgtgacca aggttctatt aaacgcattt ggtacgtcac tagcagatcg
     6061 acatgttcat attcaaactt ttctattccg taaatctgat aaaatatttt ttcttcgttg
     6121 ttactataag gaatcactgt tccgatctta aatatgtttc gtgtgttgtt tacaccaatt
     6181 tcatcagtaa tcttgtgtct atattctaat aacacgtatt tcttatacct gcttgtcaga
     6241 tcaggtgaat agttaacaga ttgacaaacc aagtcactgg ttatacgtgg atcacctatt
     6301 ctgttaattc tcgtgttata gattccgata ataatatagt ttttatcttt atattctata
     6361 gtttctccca cttctaactt ttgattaaag cctgttctat ttgcttcaaa aagttctagc
     6421 aatctttttc acctctactg ctactgccaa acaaatttag ctttatcatg ccatgtttca
     6481 gacattactt ttttaccatt ttcgtcaaat atcaccgctt cccactttgc tgttccatcc
     6541 ggctgtttac aaatatctgc aaaccatagg atcgtttttc gatcgttgaa tttccttact
     6601 ttcttctcaa atttattgat ctgaactcta ttttgattgc tgttccaacc tactaatagg
     6661 cggttatctg attctgtacc atgaaaacca atgggaacga tgtgggttct atcaaatgct
     6721 tcttttcctt ctggatagag cattggtcta agtttcctac gtttcaagtc atctcttgct
     6781 tgataccgcc atgccgattt tggattgaat ttaatagcta tttgatgctt gcttttgtca
     6841 tggtaaaaat atgcgacatc ggcttcagct ccaagcaact tttctcttgc actatttgat
     6901 tcaaacggtt tattcacttc taaaaccttc acccctaaaa tgccatgatc aagcatctgt
     6961 ttaggagtta gtaccgcttt tttatctctt ttcttcgctc gtttcacaac tacttcatct
     7021 gtatatttat atggagtaag ccatcttaaa aaagaactga cgatgatcat ttatgcacct
     7081 gtttttttgc ttttaacatt ttttgcatga taggtgaagt acttccattt atttgtttct
     7141 tttcagattt atcagttgtg atcactacgt tttctgagcc ttttggatca ttagctttag
     7201 gtttggtttt agatgcaatc ggctgtttat tattcgtgtt gtttgaggtt tgcggttcag
     7261 gatcagcttt tcctaatgcc cttaaaactt tttctagttt ttctgcttgc agtttttcta
     7321 gtgttttagc tccctgctcg actttttctt ctttgttctt ttcaagctta atagcaaacg
     7381 cactcttctt ttcaattgat ggctctttag atgatggatt tgctaaaggt gtgttcaaat
     7441 ttcttttagc agattttctt acaggagctt taccaattcc accaaaatcc tcgtcttcac
     7501 cgccctcacc acgagtttca gctacgattg ctcggtaatc aacatattct ggttctggca
     7561 tagtatttct aaggtgattg aagattgttt caatttcttt gttactgatg taagctccct
     7621 gcgctctaac catacttcca tcaacaagct taataagcat atctcccttt ggacttagac
     7681 tttctccacc gccttcatct aatacaatcc tactttctaa atcacttgct accataaggc
     7741 aaacacgtga cggaaaattg gctttgataa taccagtgac aactgttgat cgaggagatt
     7801 gtgttgctaa gataacatga atacctgctg cccttgccat ttgtcctaat tgaacaatca
     7861 aaccttctac ttctttatgt gttccaacca actgtgaata ttcatcaact aataagatca
     7921 catagggaaa tctatctaat ccattttttt cggcatattc gttatatagc gtgatgtttt
     7981 gtaaccctgc tttttccatc agtttgtaac gttcatgcat caagatcaca agatattcaa
     8041 aaaaatcata agcttcatcc atatctgtaa ttggatcagc caacatgaac ggcaattttt
     8101 tgtatcttcc aaattcaacc ctttttgggt ctacaattcc tatttttaat tcatcaggcg
     8161 ttgagtgaca cattactgta acaagaattt cattcagtaa tactgatttc cctgatcctg
     8221 tcgttcctgc aaccaacatg tgtggcattt cgctgatgca aaaatgagca attgtgccat
     8281 caggcttaat tccaaaaata gcatctgttg ggtctttagc ctttgtgttc aacatattga
     8341 cactgaattt tccattaagc tttatattag gttctttatc tagcaaaata gattcagctt
     8401 tttcggctct ctttttgact ttctctaata atttcatagc catttctatt cctcactttt
     8461 ctcggtactt ttctgtttgt gaactggtag tttgtatttt ctgtttctat ttgtgatgtc
     8521 ttctaaaaca cgctattgat cattgatggt acatcaattg gaatacgatc attttctcct
     8581 aatggtaaag taatttgaat acgccctgcg ttgatccgca cgattacacc tgatatatgc
     8641 aagtaagttt caatttcttc tttcattgag cttagttgaa tttttctggt acgagataaa
     8701 gtaaacaaat attcaataga tgtattccca acgtgcatat ctgttagact tgcaggtacg
     8761 tcagcagaag atagataaag ttctaaagat tgcttttgag tttcggcata ttttctagct
     8821 ttttcggttt ggttttggat atttttggaa ttatcggcaa agagattgat tggaaaagaa
     8881 aattgtgaat ttgaaactgc gattgaagat gttgctcttt ccgtttcttt gtttttggta
     8941 cttactcttt ccttacgttc tctttcaata tttactgatg ctcggaaaat gaaataatcg
     9001 cctgaaactt catcttcttt ttcaccaaat ttggctcgtc cgcttgttgc tcttgtcagg
     9061 atcgacccta aatcttgaat ttgtcgtaaa actgtttctc taactttctt cccttttggt
     9121 tgcgggattt ttacgatata ttctcgaata tcttcattga cacttagatc acgtcttgta
     9181 tgaacagaaa ttttgatatt tttaagcgtt gatagcacat cggattctaa ttgcgttttg
     9241 tttcgttttc tttttttgtt gttcgtttca tctcctataa tcttgtttgg atcatacttc
     9301 acatccatag cttctagagc ttctcttttc aaccttctac tttccatatc atcaaaaaat
     9361 ggtctttgtt ctccatagtg atgcctgaac atcaaagtaa ccaaatatcc aacaaagaat
     9421 accgttcctg cttctaatac taaaatagag atgtctacca agtgtagctt tgcaaaaaag
     9481 ctttgtacat tatatgaata acttaaataa ttgggaaaaa acgcattgag taactcttta
     9541 aacatctctt ttaatcctaa atcatctgtt ccgttaacat catttaacga gacaagaata
     9601 cttactaagg tatctacaag cagttgtaca acctttgtta cccaatgcaa aatatatatg
     9661 ccaattgcac caactaccaa tgcacgtcca accagatata ctgatttaga aaaactggct
     9721 acaggataat atttttctcc atcgcttcgc ttatattcct ttaattcaat ggatttttcg
     9781 ctcatctgaa tatctcctta ttttctttct gattttggtt catcgtcact tatgctgtcc
     9841 tcacacatct gcgtatcttt aggaatgatt actctgattt cgtctaacat ttctctctca
     9901 atgatcatcg ccccttttgt actaacagtt ttctctgata attcaacaca tttccctatt
     9961 tcagaatctg gatcaaaaat agcctgttct acgtcaaaat gattcttagc tacatacacg
    10021 atactcattg tttctaactc tctagcaact gaatatttac ccttcttcag aagatagtag
    10081 ccatttcctt tttttattcg atttgcaact gatttatcta atccattgat gcttattgtt
    10141 tccaattctt caccttctca atttctaatc tcttacccaa gtaccatttt gatagttgat
    10201 gttgattcct ttttgattgt taaatacata aacattaagg tttaccgttt ttccattatc
    10261 gttaactgag aaagcttcga catgagaacc acgaggaact aattcattcc ctttataaat
    10321 tggatcaact ttatacaata ctttttcatt cttattttta tagattgcat cacgtacaat
    10381 cgtttctgag taagccatcc ctccattctg atcatttttc ccaacatttt gcgctctcgt
    10441 tccaagtgtt aaattgtgta cctccatatc tcctcctagc gaccatgcta atgaatgact
    10501 tttgttatag agataacctt tatagccttc tagctgagtg atttcattgt ttgaatcttt
    10561 gccgacccaa gtatctctct cttgatcaaa tactccatcc ttgtattctc ctgcaatatg
    10621 gacagaactt ttgaaagatg gacgttccac atttttagat gagtgagaat ttacagcatc
    10681 ataggtgatc aacgcttcaa catctcctgc tcttccaagt tgatctaaat ctgcataatt
    10741 tacccaaaag gtttgactgc ctttttctga taatgcttca tggtcttctt ttgtcagtgt
    10801 atatttccca tcattaatgt ttacgatatt atttggatat ttgtttggat cataacgtat
    10861 ccctaaaaat tcttttgatt cactgtctgc tttattacct acctgaacac tacttttgat
    10921 ctgaaaatca tgttcctgag tactgttatt ctgaaagtaa cccttgccac taagaccaac
    10981 cgcaactaaa accaaaattc ctaacaacga tcggctaaac ttcttctttt tcataccacc
    11041 attcctttta ttgtgttatc ataataagat aaatatataa taaactatat tgtaaatttt
    11101 gtcaaatgta tttttattta ttcccactat aaaccttgat atatagccaa tttaaaaaag
    11161 gatggcaaaa aattatcaag ttaaaaaaat aaattttgaa ccaaattatt tttaataaaa
    11221 tagctagttt ttttatcaaa aaaaatagag cttttcagct ctattcagac tgtagacaaa
    11281 ctcctaaatt tggagtgttg tttgcagttt tttgttttat actaattgta taaactattc
    11341 tgggagtgat tctaatgatg agaaaacaat cgattgaagg tagaaatcaa tttgctatgt
    11401 taacaattga tgatcttgta cctaaagatc acttagttcg aaaaattgat gctgcgattc
    11461 agtttgattt catttatccc attgtagaat ctacgtattc aacctatggt cgtcccagta
    11521 ttgatccagt ggtcttaatt aaattagtat ttatccaata tttatttggt atccgttcga
    11581 tgagacagac aattaaagac gttgaaacaa atgttgctta ccgttggttt ttaggttatt
    11641 cattcgagga tcctatccca cacttctcta cttttggtaa aaactatgtt cgaagattta
    11701 gagaaacaac tttatttgaa gatattttct ctcatattct agaacaagct gtaaaagctg
    11761 gcttcgttac ggaagacaac ctttatattg attctactca tatcaaagca aatgccaata
    11821 aacataagtt cacgaaagag atgacctacg gtcaagcaaa agcttatcaa gatgaattag
    11881 aagatgaaat aaataaagaa cgtattgctg taggaaaacg tccttttaca tgggacacag
    11941 aaagtgagtt gagtcttcaa aaaataagtc attcagatcc agaaagtggt tactatgtta
    12001 aaggtgaacg agaaaaacaa tttgcttatt ctgcacatac ttcctgtgaa gataacggtt
    12061 ttatcttatc tacattaatc acaccaggaa atgtgcatga cagccaagtc gcaatcgatc
    12121 tggttaaaca gtcgaaaaga gcttttccaa atataaagcg tgtggttgct gatgcaggat
    12181 ataagacgcc taaatttgtt cattttctgt cacacttgaa actctacccg attcttccat
    12241 atactgcacc tcatggagtg aaaggaatgt ttcgtaaaaa agactttgtt tatgatgcct
    12301 attttgactg ctatttatgt cccaataatc aaccgttgtt attttcaact atcactcgtg
    12361 atggttatcg aacctataaa tcaaatccaa aattctgtgt gagttgtcca ttacttgaaa
    12421 attgtacttt gagtacaaaa catcaaaaaa tagtgactcg acatatttgg gctgatctta
    12481 tggatgaact tgaacaccaa cgccatacgg cgttgaatag agaaatttac aaaaagagaa
    12541 aacaaaccat tgaacgaata tttgcagatg caaaagaaaa gcatggtatg cgttggacga
    12601 aatatcgagg tcttgaaaaa gtcgccacgc acacgatgct tacgtttgct gccatgaatt
    12661 taaaaaaact agccacatgg ctatggaaaa caaaaaagcc tatggctttt tatttcaaaa
    12721 ttatgcaaag actaacaaaa aggagtggct taattaccaa ctcctttttg tctacaatct
    12781 gaatagagct tttcagctct attttattca atatcatttt gatcaattaa taatcgtaca
    12841 ctccaattaa ccgagttttg atcactcaaa tatttataat ctttgtcatc aaaaaccagt
    12901 tgccaaactc tggtacgaac agtttcttcg tccacttcct catctgaaga gtattgcgat
    12961 aatgcttctt caactatata ctttacaact tcctcaaaat cagtaggaac tttatcaata
    13021 accacttcat agtcttcttg ttttacgata ttgggatcaa tatgttcatc caaacatacc
    13081 tcagtgagtt tttctttcac ttctgtatct ctttctgttt ttaggtactc tgcatagtct
    13141 tccttagacc attgtttttc aagatcatcc gctatacttt taataaagga ttctttattt
    13201 aaccaattat ctgcggtatc tataccttca aactcaaaag ctgttccttc tatacgtgat
    13261 ttgacgaaag cgtcaatttt cttgtattga ttattcattt cccatgaaac acactcatct
    13321 tcttgcgtaa tatcataaat tttttcggaa aaactaacta attcattcca cgatttatcc
    13381 tcggcaactt gacttagttt cgatgcaata aaaggaaatt ttgcttttaa gtcatccata
    13441 ttcttgatct caaattcgat attttcttct cctacagctg aaatccatgc tgaaagattg
    13501 attgcaatat cgctaaagtc ttctacgttt tcgacatttc ctgtattatc ttttattact
    13561 gcttctaaag ccatatcggt tgttgcactc atggtttgat ctcctctcat aatagagcaa
    13621 aaagccggtt taaccgactt tttgcatggt acgttttgtt tagtagtttt ctgcttgtgt
    13681 ttatgttgaa ataatcccta tagctcttct tttaatagtt cttcaacttt atctcttaac
    13741 tctccctgac cactctctaa tagatttaaa tagctcatca gaatatccgt ttcatacgtt
    13801 tttaattcag ctctatactc actttctgaa tatgatcgta aatacccatc attgctaaat
    13861 gcaactagat catcttgaaa gttccattct cctaaatttg ttttactcac tgcttcccat
    13921 ggactattac taaacatttc attaaagaaa tcttcgtcat tttgatatac cttcaattca
    13981 ggaaaatagt tgctgtcttc attgatttct tgcaccattt ttccaagttc ttcttcagat
    14041 aatttacctt ccaataattt tactttttct ctgtcatttg cataagccat ttgaacctct
    14101 cctttttctt tttcaatttc ttcaggtgtt tgttttggcg taactttcaa atctattcca
    14161 ttgcctatta gacctattag ataaccatag aacgttttaa agtcacttgt agcaagagat
    14221 ttggcatcag ggtatgtatc ccctccaatt ttatctaaaa caaaatcagc aatcgttacc
    14281 caaggagaat atatcgtaca tactacatcc catgtagcat cttcaatatt cgtccatgtc
    14341 ggaaagtcat tttcattcaa atagtctttt ttcattgttt cgatcagagt ttttccactt
    14401 tcgttttggc ttagatattc aacaaattct gcaccatcaa acctgctact aaatagttta
    14461 gctaatgagt ctaaagctgt ttttgatact cccaccatta ctacacctcc tattaatatt
    14521 ttacgatgac ttgtttttca ttcacaaata agattttcta catcttatct attgctttat
    14581 agcttttcca tacattattt ctaaatacaa ccaataaccc tattaaatta agcttttgag
    14641 tttatctttt ctaataatat tctgataaat attctgataa aaaaatttat
//
LOCUS       DANJMI010000063        14426 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000063 DANJMI010000000
VERSION     DANJMI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.063"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(602..1786)
                     /locus_tag="PWU81_002459"
     CDS             complement(602..1786)
                     /locus_tag="PWU81_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL1111127.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1811..2818)
                     /locus_tag="PWU81_002460"
     CDS             complement(1811..2818)
                     /locus_tag="PWU81_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL1111128.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(2990..3349)
                     /locus_tag="PWU81_002461"
     CDS             complement(2990..3349)
                     /locus_tag="PWU81_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111129.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(3321..3692)
                     /locus_tag="PWU81_002462"
     CDS             complement(3321..3692)
                     /locus_tag="PWU81_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HDL1111130.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(3709..4011)
                     /locus_tag="PWU81_002463"
     CDS             complement(3709..4011)
                     /locus_tag="PWU81_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111131.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(4028..4498)
                     /locus_tag="PWU81_002464"
     CDS             complement(4028..4498)
                     /locus_tag="PWU81_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDL1111132.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(4473..4751)
                     /locus_tag="PWU81_002465"
     CDS             complement(4473..4751)
                     /locus_tag="PWU81_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDL1111133.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(4748..5797)
                     /locus_tag="PWU81_002466"
     CDS             complement(4748..5797)
                     /locus_tag="PWU81_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HDL1111134.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(5754..6683)
                     /gene="tadA"
                     /locus_tag="PWU81_002467"
     CDS             complement(5754..6683)
                     /gene="tadA"
                     /locus_tag="PWU81_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDL1111135.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            6815..8569
                     /gene="ade"
                     /locus_tag="PWU81_002468"
     CDS             6815..8569
                     /gene="ade"
                     /locus_tag="PWU81_002468"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDL1111136.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(8667..9329)
                     /gene="trmB"
                     /locus_tag="PWU81_002469"
     CDS             complement(8667..9329)
                     /gene="trmB"
                     /locus_tag="PWU81_002469"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL1111137.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(9399..10181)
                     /locus_tag="PWU81_002470"
     CDS             complement(9399..10181)
                     /locus_tag="PWU81_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDL1111138.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(10385..11596)
                     /locus_tag="PWU81_002471"
     CDS             complement(10385..11596)
                     /locus_tag="PWU81_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1111139.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(11589..12326)
                     /locus_tag="PWU81_002472"
     CDS             complement(11589..12326)
                     /locus_tag="PWU81_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1111140.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            12464..12898
                     /locus_tag="PWU81_002473"
     CDS             12464..12898
                     /locus_tag="PWU81_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDL1111141.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12891..13235
                     /locus_tag="PWU81_002474"
     CDS             12891..13235
                     /locus_tag="PWU81_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL1111142.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            13415..14425
                     /locus_tag="PWU81_002475"
     CDS             13415..14425
                     /locus_tag="PWU81_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL1111143.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         4588 a         2936 c         2434 g         4468 t
ORIGIN      
        1 cgagtaattt tctagtatga ttatccattg agagactcct ttggtagggt gacgttttgg
       61 tcgactaaat tttaccaaaa gtgagtctct ttttgagttg aaacgaagaa aaaagtgtta
      121 gtagaaaatc gctttcgatt ttccaccaac acaaaatatt atagaaccgt tttttcgcaa
      181 caaaaaactt gattttcata cctgtgcttt cacgattcag gcaaagtaca ggtggaaaat
      241 caagttttta ttattcgatc aatgaatgtt tcggatcggt tttctagcta aacataaagc
      301 gaagaataaa gtcgacagcc cacgaggctg atctaaaaat gttttttctt tttacatatg
      361 taatgtccct tataaagatg aatgaaaata aaatattggg attttcctga aaatcaataa
      421 ataaaatgcc agtcttttcc cttttgacca cttttccaag caaaattccc taagaagatc
      481 ctgtaaaaaa gaagggaaat tgaagttaaa ttaccatttt gcggtcttct ccgcaaaaaa
      541 aacacatgca agaattccct cttgcatgcg tttcatcttt tatcctaacg aatgctttgg
      601 cttagccgcg cagacgctcc acatcacggg caatcattag ttcctcatct gttgggatca
      661 ctaatactct tacttttgat tcttttgtag agatatctac ttcttcacca cgcaattcat
      721 tcaacgctgg atcgatttca caaccaaacc aagacatacc tttgatgatt tcactacgta
      781 catgtccgtc attctcgccg ataccagcag taaagacaat tgcatcaaca ccattcatcg
      841 ttgtcacata accgccgatg tattttcgga tgcggtccac gaaaatgtca taagctaaac
      901 gaatatgttc tttttcatag ttcttttcaa gatcacgcat atcactagac aatcctgtta
      961 ggcctagcag accagatttc ttgttcaaaa cattgatcat ttcatcaata tcaatctcta
     1021 atttttccat taaatatggg agaacagctg gatcgatatc acctgaacgt gtacccattg
     1081 tcaccccagc taatggtgtg aatcccatag aagtatccat ggatttacct ccatcgactg
     1141 ctgtgatcga tacaccattt cctaagtggc aagtgatgat ttttagttct tcgatcggtt
     1201 tgttcaataa ttttgccgca cgttccgaca cataacgatg acttgtgcca tggaagccat
     1261 attttcttac tttgaaatct tcataataag acaccggtaa gctatataaa taattttgct
     1321 ttggcattgt tgagtggaaa gatgtatcga atacagccac actcaaaatg tcaggtaaaa
     1381 tctttttaaa cgctttgatc cccattaagt ttgcaggatt atgcaaagga gcaaattctg
     1441 ctaattcagc aatgcggtcc attactttct catcgatcac tactgaatcc ccataagttt
     1501 ctcctccgtg aacaacacgg tgccctactc cggtgatttc atcataagag cttaaaattt
     1561 gcagttcaat caactgatct aacagcattt ttactgctac ttcgtggttt tcgatatcca
     1621 tgatttgttc gaatttttga ttatcgccgt acttgatggt aaaaatagaa tcttgtaaac
     1681 cgatacgttc gacgatccct ttcgcaacta cggtttcttc tggcatttga tataattgcc
     1741 atttcaaact tgaactccca gcattgattg caattgtttt agacatatga ctctccttct
     1801 cgttgaatga ttacagagca gatgatttcc agtttttaaa ctcgttgaaa aattccgtca
     1861 ccttctctgg ttcttttaat gaagcaagtt tcacaagcaa gacttctttt acttgttgtg
     1921 cttgtggtcc ttttttctgt aagatcaaaa tacttttttg cgattgttta ttacggaaca
     1981 attcatctgg caattgaatc attccttgaa gataaccttc ttctttaaac cattttttga
     2041 tttcttcact ttgatccgtt tctaaaaatc cactcggcat caaaaacaaa ccaaaaccat
     2101 ccggcttcac atatttcatt gattgctcta gcaataaatg atgtgcataa ctatggcctt
     2161 cttcggtact tgttaaaaac tcttttgctt tttcgtcatt cggataataa ccgattggca
     2221 aatcgctgat tgcaaaatcg acaggatcta ttaataattc ctgcaacccg tcttgatgaa
     2281 agtattgaac atttgcttgt gtcaaatcac ttgtagaagc tgcgacagcc aacaatgtat
     2341 catcaatatc cacaccaatt ccttctgttt ggtaccccgc attcgatagg tttaaaagaa
     2401 ctgttaataa caagttgccc attcctgccg caatatccag aatcttaacc ggtgcttttt
     2461 tatttgtgta tagttgttca ataagaaaaa caaataaaaa accgatacta tcaggagtga
     2521 gttgatggtt cgcctgcaaa tgttctgtct ggctgccctt caataaaagt aattgagaca
     2581 gtctgcgcca ttcttctggc tcgaaagaaa gtttcttcaa ctcttcataa agagctgtga
     2641 tccgttgcgt tgtctctttt gtcggcactc catccaccac acgtacttga taatcatcta
     2701 ttaaattttc tgcattttcg acataggctt ctaaaaagga agttcccaat gcattctgta
     2761 aaagctgaat agcttccagg ttttgattaa acccttgttc cattttttct ggtaacatgg
     2821 tgcacctcat ttctagatat attataacaa ttttttcacc tgtttaattc tatcgaacaa
     2881 ttgacaaaat ttcaatcgtt ttcactgttt tttatttgtg aaacggcaaa cattttttta
     2941 ttaaaaacag tgttataaca gaaaagaggg cgttttcacg aacattccat cattctgtac
     3001 tagtatctgt atcatttttt acttcttctg tgaaccgaaa gataaacggt gagacttcca
     3061 cctctaagaa tagggactgt tgtacccact gatatgttac agatccagtt gtgtagtaaa
     3121 aaactccttc cgaactttcg gcaagcgtag actgttccga taaagccatc tgcttcatta
     3181 tttttgctat ataataattt ttgattcgta tattaagatc cattgacaaa cggtagcttt
     3241 ctaatgctgc catcaataaa aacgtacata aaagaaataa aagcaagaca gaaaataaaa
     3301 tagagccctt ataacatttt ctactgatcg ttttttgtcc atatggcata tccatcttct
     3361 ccattttcaa agtgtacata aaaagaaatt gtgtgttctt tttccaataa ttgaagctca
     3421 gtgatacttg ttaacatagg ttgatgtccg ccggaagttc ttcttttacg tatcattgcc
     3481 tgatatcttt cgatgatata ctgtttatcc tcgatcgtgt agtgcagttg ctgatttttc
     3541 acagagacat tgtgactgct cttgagttct tcttctaatt gtgctaaaaa tattagccac
     3601 tctttttgat ctttacgctg caaaaattca cttacttgga tggtttgctg aatcaaaaga
     3661 ttgaatgtga gcaatagact gctcaatatc atcagtccaa ttagacattc aatcagcgta
     3721 aatgctggaa ttacttgttt tttcaatact gatcgcttct ttctccatat tgacaaacac
     3781 tttttcgatt actccattat tatcgacaat ttggatatcg tatgggatca tttcttccct
     3841 tcgctctggt cttttgtgat acatccgctg cgtcttgata tcttcataaa tcattcgaat
     3901 aaatagcagt tcctccccac ttttcttttc tgcctgaagc aaaaacagct ggccaaagct
     3961 cactaagtag acacagacag aagcgatcat aagcgctgta agggactcca acaaaacaaa
     4021 tcctgtttta ttcaattctt tttgtatagc gaccactccc tatctggaat tgatacgtaa
     4081 tcgtccgctc tttttcttcc caataaaatt gataagcttt tagtgaactt tcattcccgg
     4141 ttctagcagc aaaagtaata gaagcatggc gtttcaaggt aatctcatca ggaatatcta
     4201 atacttccca atttatttta tctggattcg gaacatgaaa aatgatttga ttctcttcac
     4261 tattccaata aaagcctgtt tggatctgat tcacaatagc gattttttgc gtagaataga
     4321 ttcgtttttc aaattggccg aaaaattgat agactgccaa ttcattcttc catgcagaaa
     4381 atgcgagaac tggaaaagaa aggatcgttg taagtaataa aatcagtaag agtgattcaa
     4441 acaacgtata tcctgatgct aaaaccttac cttcaaggcc gctgatagat ttctgcatgt
     4501 tctcgtttga tatagccttc attcaacaat tcctccacag taggaactct tccattattt
     4561 tgaagcgcat agagctcttt ttgtgtgtct accaactgga tgatcgcttc acgactttct
     4621 tggtctacat gattcctata acgcgacaaa ttagggacga acaacaacac taatacactg
     4681 atgatcagta agacgattag catttcgatc aatgtaaaac cgctataata agatattttt
     4741 ctctttttca tggcaaaacc tcctctatgc ttccgtaaac aggtaataaa acagcggcat
     4801 aaactaataa aatcaaagcc gctacgccca aaaaaatgat tggctgaata aaatgcatcc
     4861 agtgatgaag acgtttgaaa agctgttttc ttgtccattc gctgtaaaaa atcagttctt
     4921 tacctaaact tccctttgcc tctccctgta aaacaatttt actgaattct tctgatagaa
     4981 aacgataatt ctggaactgc tcagccaatg acatacctgc ctccaatcct tgaatcaact
     5041 tagaggcaag atgctgaatc agactttctt gtttcgtttc ctttaagcag actatgattt
     5101 gccttaattc gaaaccttca tgaaaaagct ttccaatctc tagtgacaaa taagccgatt
     5161 gatacaagga atatatcggt ccaatcaata attgtctgct tatccattca ctctgatcaa
     5221 caggagacaa cttcatcaga cgatacctta ctagtaaagc caatagagta accgctagga
     5281 tgaatagtag aacataccaa tgaaagactt gtatcagctg aataccccaa tgctcttgca
     5341 tgaccattcc tgaaccaata agctgaggca aaacgaacaa ccgcatacta aacaagatac
     5401 ctagcaaaaa ggtgagcaat agcatcggat atgcaagcag ctttttcaaa tcattgcgaa
     5461 atccttcaac aagccgaaac tgtttggcaa tttccaacaa agttgtcggc agattcccat
     5521 gacgttcagc taactcgatc tgtaccagtt gctctgctcc atatcctatt tgcgaaagta
     5581 cctcactaaa cgcttgccct tcctttagct gatgttggat atttgccaaa ataacaggtg
     5641 gaaacgaacg gatcgtgagt aataaataaa gggattcttg cagactaaaa ccagataaaa
     5701 gcagttctcc catggtttgt gcaaactggt tttgctggct tcttgttaat cctttagaat
     5761 agttctgact gccaaatatt ttcctgagct tcttcataac tttcctccca tgattttttt
     5821 actgattctt cctccataaa ttgataactc cataaaacag aaggatggtg attggcatct
     5881 tccaaaagtt cttgataaat gacaccagct aaacattcag atagctcttc ttttttcttt
     5941 gtcagttcat ggattctgac caatgtacct tcaagattgc gtgcatgtac ggttgcaaaa
     6001 acccggtgcc ctgttagtgc tgctcttacc gcagcatgtg cagttgattc atctcttatc
     6061 tcgccaatga ttagcaagtc tggacgatgc cgtaatgcga gtctcaaaag tgtatcataa
     6121 gtctgtgcta ttttcggatt gatctgtagc tgcagaaaaa acggttcttc tatctctaca
     6181 ggatcttcga tagtaatcac ttgcaatttt tcttccaaag ccaaccgata cattagagaa
     6241 gtcttgcctg atccgactgg tccgctgaaa aggtataatc cacgtctgac ggttttctgt
     6301 aaaatagttg caaaatctcg agaactaaag caacgatggg gtgtttcaaa agtaccgtat
     6361 aatagccgaa tcactaaact ctctcgttga aggtaatctc ctacagaaga caaccgcaga
     6421 cgaacttcct gtttttctaa agaataagta aatgcaccta actgagcttt tcttttttct
     6481 ccgatatcca tctgtcccaa atacttaaat cttgagatca gttgctgtcc atctgccgaa
     6541 gagactttct tccagaatgt tcgttttttt ccatagcgaa aatagatgta aaatacttct
     6601 gaagctggca atatatatac gtcttgggca tgtttggcaa aactttcact tatcatttga
     6661 tccgataatc gttttatttc cattattctc ccctccacaa aaaacatacg tctttttgat
     6721 ctttttgttt caaataaaat gaatttacaa aaaaatattg gcaaaaaccg aatttatgtt
     6781 atacttataa aaatttatct tgggaggcac tccattggaa acaacattaa ggaaatatat
     6841 cgaagtagca agtaaaaaaa agcctgctga cctagtgatc aagcatgcta agatcgtcaa
     6901 cgtctttacg aaagaaatca tggaaaaaga tgtagcaatc tgcgaaggaa tgatcgtagg
     6961 aatcggtgaa tacgaaggaa aaagcgtaca taatgctaat gggcaatatc tagtgccagg
     7021 attcatcgat ggccatgtgc atatcgaaag ctcactttta tcaccaagcg aattttcaaa
     7081 agtatccttg cttcatggag ttactactgt agtgacagat ccccatgaaa taggaaacat
     7141 tgctgggagt acaggtcttg agtttatgat agaagatgca agacaaacac tcatgaatat
     7201 cttttttatg ctaccttctt gtgtacctgt aacccctttt gagaccaatg gtgcaaattt
     7261 aaatgcaaaa aaattagccc cttttttaca aaaacccgaa gttttaggct tagctgaagt
     7321 catgaactat caagcagtag caaataatga aacagacatc ctagaaaaga tccaattgat
     7381 gcaccagcat aagaaaaaaa ttgacgggca tgcagctggt atcggcatgg aagagttaaa
     7441 tgtttatcct gcagcaggta ttcggacgga tcatgaagct acaactgcaa aagaagctaa
     7501 agaacgtttg gatcttggta tgtatttaat ggttcgagaa gggaccgttg caaaagatct
     7561 ggcatcgcta ctaccggcga tcacacctga aaattcaaga agatgcttct ttgtcaccga
     7621 tgataaactg atcaatgatt tagtaaaaga aggatcaatc gatcatatcg tccgtcaagc
     7681 tattgcactt ggaatcgatc cattacaagc cattcagatg gcaacattga atgcagcaga
     7741 atgttttgat ttgaaaacac taggtgcgat tgcccctggg tatcaagctg atttcttctt
     7801 aacagacgat cttaccaagc tgccaattca tgaagttttc tataagggaa cattagttgt
     7861 tcaatctggg caactccaag accatttatt tgctgatcat tcaaattcat atacgtgttg
     7921 tctgccaaaa ctaaacgctc aaccattaaa tccacattcg ttagagctgc ttctttcttc
     7981 ttcacaagcc catgtgatcg aaattatccc gaatagtcta gtgactaaag atttggtaga
     8041 aaaagtggac cgacaaggcg aacgattcat tccttcaatc gaaaaagacc aattaaagat
     8101 tgcggtgatc gagcgtcacc atcaaacggg aaacattgga ttaggcatag tgaaaggatt
     8161 tcagctgaaa caaggagcga tagcgacaac gattgcccat gactcgcata atctagttgt
     8221 cgttgggaca aacgacgagg atatgctgta tgctgctaat cttttgattc aaaaaggtgg
     8281 cgggatgacc gttgtcaaca atcagcagga aatagcttgt gtttctcttc ctataggcgg
     8341 aatcatgaca caggaacctt tttcgatcgt ctatgaacag ttgaatgaat taataactaa
     8401 agcacatcaa ctaggagcag ctaaaacctt tgatcctttt ttgacacttt ctttcttaac
     8461 attatctgtc atacccgaat tgaagatcac cgacaaagga ttattttcat tttctcgctt
     8521 ccaattgatt ccaccttgtg gaaaaaccga tgaaataaat acattttaaa tcaaataaat
     8581 cccagaaaat acattacatc tgtaatgttc attttctggg atttatttat ggatattaat
     8641 gtttttatga aagcagaaag ttagtttcac agacttttct ttttggtctt atcttgaaat
     8701 tgagcttcta ctcgataaat acgttgacct tttgaagaaa atttttcttc atactccgtc
     8761 ataatattgc cttcaaattg actttgatgt aaatctaacc atacctgttt gaggatcatg
     8821 ccatattgag aaaaacttgc cagagaatac tcaaacaatc cttgattatc tgttttgaaa
     8881 tggatttctc catttggacg aagaatctgt tcatctacag caagaaaagt cttatatgtc
     8941 aatcgccgtt tttcatgacg tttttttggc catggatcgg aaaaattcag atagatttga
     9001 tctacttcgc tgtcagcgaa atactctgtc aacgctgatc catccacatg tagaagtttc
     9061 aggttaggaa gcggttgttc gatcaacttg tctagtgcaa tcgaaacaac actgacctgc
     9121 atttctactc caatataatt gatttcagga tgggcttttg ccattccagt gatgaactgg
     9181 cctttcccca taccaatctc aatgtggatt ggatgatcgt tttcaaacaa ttcattccat
     9241 tttcccttcc acaatgttgg atcactaatt acaaaattcg ggtgagccgc taacatttcc
     9301 gctgctcccg gacgatttcg gacacgcatt atttatctcc attcctttcc tctaattaaa
     9361 aaggtctggc caaatctccg cagagtttgg gccaaacctc agccactgaa aattcgtttt
     9421 aattgtaaaa tttcttcgtt catccgtcga tgatcttcac gtttgcaata tctggtgatt
     9481 tcttggagca aattcatggc cgcataccaa tgaaattttt caagcatttc gtcagtcggg
     9541 cgtatgccat aactgatcag ccactgattc cagctagaca gtggcacata atttcctaaa
     9601 atcgttgcaa tatccaccgc agggtctgca aacatgattg aatcccaatc gactagatat
     9661 aaataatttt tacatactaa ccaattacga tggtttacat ctccgtgtac tactgataag
     9721 ttttccttat ctattgacgg gatctgctca cgaagaaaac gatggacaat ttgcagaaat
     9781 tgatttttct ggagttcttc cggtaattct ttttcatatt tttgcagcat cttctccggc
     9841 gtcatgacac gaccaccgat acgggtcagc atggttttaa gagaatttga atgatgtaaa
     9901 tggtacaaaa catcaatcac gtcgtttctt tgaccaatct cattaggagt cagcaagcga
     9961 ccttccagcc actcttgtgc tgtcaaagta tcgccattcc ccgttcgttt tgtccaaaca
    10021 agttttggtg taattccttc ccttgaaaga gcagccaaca tcggagtcgt gtttcgttta
    10081 ataaagactt tgtcctcatc acgcatgcct atgtatgttt ttcctgtgtc gcctttgatg
    10141 ggacgtaacc gccactcttg gtctaactga aactccatca tgcattctcc cgtcctctta
    10201 ctctttgttt tgcagtactt attattctag tcgcctgaac actacacgtc aagcacatat
    10261 acgttctttt tcatttaatt gccattcaaa aaaccagctt ttctcaaaaa aaatagaacg
    10321 aacagcattc ttactgtcaa cgctctattt ttcattctca tttttaccct acacagcttt
    10381 gttttcagtc tgacatcttc ttcaaacgag ttggtacata tagtttaaca aagagcatag
    10441 taacaatcaa ataagccgct agcggaaccc atcgctgctg cccatctaat acgactgcag
    10501 caatcaaacc aaaaataacc gacgcgataa ttaataccca tgtgagtaga tactgaatcg
    10561 cctgcttctt ctgattctta tggattggat aaagctgaac gagaaccata tagcggaact
    10621 ggttcgctaa aggtaacagt tggaaaccga tcaaatataa aaataagaaa ccgataatca
    10681 gactaaaata aaagtcgact actccggcaa tcaataaccc acctataatt actaatcgga
    10741 aataaagacc actaaaatca gaacctcttg ccaatcttct tgcaaacaag taaagataag
    10801 tgttttttga ttctctttta atatgagtca atataggatc caaatagcgt cttcttttga
    10861 tctttgccgt aatttcagga acatctgtga ataaattgat aaattgatag atctgatgca
    10921 acctatgttc ttctacatgc accattttct cccaatccaa tcctttttct tgttgcttcc
    10981 acaaaaacca gtaatataga cttgcctgta ctaatgcacc tgctattcct gcccatatta
    11041 gccaataaaa tgatagaaac aaaaagatca gagaagaaaa aaaccagcct aatttccaat
    11101 aacgaataac ttgttgatct agtcgaaaca attctgctct cttgatttgc agatgactga
    11161 atttcaaact ccataatatg atcagataga cgaaaaacat cgaaaaggat tgctccgtac
    11221 taaccaccac taatggcatg gccaatccgc atgccaatat caaaaagatc aacggaaaaa
    11281 tgcaagaata gcgaaaagct cgttccaaat aggtttgcat ttctttttct ttaggaagta
    11341 aaaaaacaat atctgctgcc tctgcaaaag tagccatctt acccacgaat aaacagagca
    11401 tccaaaatac agagattatg agccctccaa ttgcaaacgg tggctgcagg gttttcaacc
    11461 aattagaata atataatccc acaccgccaa tcaagaaaag acaaactaaa acaaaatgat
    11521 cgttcaatac ataacgcata tatttcatca ttttcttttg atgtcttgct aaacgttttt
    11581 gaaaaaaact actcatgtgc ctgctccttt gttaaagcaa tatagatttc gtccaaagaa
    11641 gaatccggca gctgaaattc ttttctcaac atctcaagag aaccgtctgc tcgtatctct
    11701 ccgttatgta agaccacaaa acgatcacaa tatttttctg cagtagctaa aatatgcgta
    11761 gacatcaaaa taccggctcc ttcttttttc atttctacca tcaattcaag caatgcattg
    11821 atagctagtg gatccaagcc taaaaatggt tcgtcgatga tatataaaga aggctcgatc
    11881 aaaaaggcgc ataagatcat cactttctgt ttcattccct ttgaaaaatt agctgggaac
    11941 cagtcaagac gattagataa ccgaaaagtc tccaacaact tttctgctct tttcattgct
    12001 atatccatag gtatatcgta ggccatcgca gtgacttcga tatgttctct caatgtcaat
    12061 tcttcatata aagaaggagt ctctggaata tacccaattt gtttgcgata ttcttccgta
    12121 tttttcttta aggatagatg attgatttct atcgttcctt ttgaagcttc cagcagaccg
    12181 ataatgtgct tgatggtcgt acttttccct gctccgttca gtccaatcaa ccctactatc
    12241 tctccaggct gtacatcgaa actgatatct tttaacacag ggagatgtcc gtacccccct
    12301 gtgacatgat cgataattaa actcattctt tttcctccgc catttatttt tatttattat
    12361 accacgaaga aaatgacggt attttgtttt tttccttaca atagctttaa cttatggtaa
    12421 agttaagctg aagtatttat tctttataga aaggatgttt tatatggaaa actgtatttt
    12481 ttgcaaaatc atcaatcaag aaatcccaag ctataaaata tatgaagacg acaaggtcta
    12541 tgctttttta gacatctctc aagcaacgaa aggacacaca ttagttgtgc ctaaacgtca
    12601 tgtagcagat atttttgaat atgatcccga acttgcagga gaggtttttt cgcgtgtgcc
    12661 taagattgct caagcattgg aaaaagcttt tccagaaatg aaaggtctta atatcttgaa
    12721 taacaaccgt gaactagcct atcaatctgt ttttcattca catatccatt tagtccctcg
    12781 ctacagcaaa gaggatgatt tttctattca ctttgtcaat catcaagata gttatggatc
    12841 ggaagaacta aaagctattc aagaaacaat cgtaaagcag gtgtcctgcg atgattaaaa
    12901 actttttcaa aggactgtta tttggagctg ctgctggcac tgtcggaggt ctgttattgg
    12961 cgcccaggag cgggaatgaa acaagaaaga aactgatcga agaattggaa gaagcaacaa
    13021 ctctgacaaa tgatttaaat aacagcctga atcattttaa aagcgcattg atcgaaacaa
    13081 aagaaacagc tgaatctgtg attcctgctt tccaagaagc aatcaaaaaa gatatcgaag
    13141 attttaaatt ccaagccgaa ccacgtatca cacaaatcaa tgaacaagta gaaaaactaa
    13201 aagctgatct gccagagtat cctgaacaaa tataataatg attattttct caacagattt
    13261 aactatataa gtgagaaaag tttgcgtatt tcctaggaat gtgatgaaat tttgaatttt
    13321 gtttgaaact accacgacaa cgtttttaaa ggaaatggga tatggtatag ttaatatata
    13381 aaaataataa ctaattacag gagtgcaaat tttaatgaaa aaaaaatcaa ttattttggc
    13441 agctactagt gcgttggctg ttttaactct agcagcttgt tcaggcgata ctaataaaga
    13501 tatcgcaacg atgaaaggcg gaacgatcac tgtatctgat ttctatgatg aagcaaaact
    13561 tgaatcatcg aaccaatcct tagtacaacg gatgatcatt tataaagtat tcaataacaa
    13621 atatggcgat aaagtaaccg ataagcaagt ggatgcagaa tacgacaaac aagcgaaatc
    13681 attaggtgat acttttgaat cacaattaga agctgctgga tatacgaaag atacttataa
    13741 agaatatatt cgtaacaatt tagcttttga agctggcttg aaagctcacg tagatattac
    13801 agatgacgat ttgaaaactg cttggaaatc tttccatcca gaagtagaag cacaaatcat
    13861 caaattatca agtgaagatg aagcaaaaga tgtgaaaaaa tcagcggatg atggagatga
    13921 tttctctaaa ttagccaaag acaaatcaac tgatacagag acaaaagaag atggcggtaa
    13981 agttaaattc gactcaacga caacaactat tcctgcagaa gtaaaagaag cagctttcaa
    14041 attgaaagac ggtgagatct ccgatgttat tactacaaca aatcctactt cttatgcgac
    14101 agaatactac gttgtcaaaa tggtgaaaaa ccaaaataaa ggcaatgaca tggataaata
    14161 caaagatcaa ctaaaagata tcgctactga aactaaacta agtgacaatg cattcacaac
    14221 taaagtaatc ggtgaagaat taaaagatgc aaatgtaaaa atcaaagatg atgcctttga
    14281 aaatgtgttg agtgcattca caacaacttc atcaagtacg aaagactcaa gtgaaactac
    14341 agcttcaaca aagagttctg acacaaaatc aactgattca acaaaagaat cttcaactga
    14401 agaaacaact gattcatcaa aataaa
//
LOCUS       DANJMI010000064        14300 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000064 DANJMI010000000
VERSION     DANJMI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.064"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(51..3725)
                     /gene="rpoC"
                     /locus_tag="PWU81_002476"
     CDS             complement(51..3725)
                     /gene="rpoC"
                     /locus_tag="PWU81_002476"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL1111144.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="PWU81_002477"
     CDS             complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="PWU81_002477"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL1111145.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8128..9096
                     /locus_tag="PWU81_002478"
     CDS             8128..9096
                     /locus_tag="PWU81_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1111146.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9114..10019
                     /locus_tag="PWU81_002479"
     CDS             9114..10019
                     /locus_tag="PWU81_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1111147.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10016..10822
                     /locus_tag="PWU81_002480"
     CDS             10016..10822
                     /locus_tag="PWU81_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL1111148.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10958..11947
                     /locus_tag="PWU81_002481"
     CDS             10958..11947
                     /locus_tag="PWU81_002481"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL1111149.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12264..12788)
                     /locus_tag="PWU81_002482"
     CDS             complement(12264..12788)
                     /locus_tag="PWU81_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HDL1111150.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13075..>14300
                     /gene="scm"
                     /locus_tag="PWU81_002483"
     CDS             13075..>14300
                     /gene="scm"
                     /locus_tag="PWU81_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL1111151.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDST"
BASE COUNT         4060 a         3094 c         2820 g         4326 t
ORIGIN      
        1 caaaaaagag gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt
       61 tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca
      121 ttttcgcttg ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta
      181 ccagctggga tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc
      241 ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat
      301 aagaaactat ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt
      361 gcaggaacgc ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct
      421 aataatgtac ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc
      481 atttgacgaa ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa
      541 acacgttgta cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac
      601 aattgttttg gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct
      661 tctgctactt tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct
      721 ttgattgtta cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct
      781 gttacttctg tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca
      841 cgaggaagac cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc
      901 gtcaactgag taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg
      961 acttcaacgt cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt
     1021 tttgtattac atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt
     1081 tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca
     1141 ggatgacgta cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg
     1201 atttcatttc cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct
     1261 tcacggatga ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg
     1321 gcagtcttca aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt
     1381 tccaagacgg atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca
     1441 ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca
     1501 cgagctccag aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt
     1561 ttttgttgga tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg
     1621 tcatctgtga tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca
     1681 ttttcaatga tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata
     1741 ccggcatgtg tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt
     1801 tctgttactt tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg
     1861 aacggtaata ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca
     1921 aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg
     1981 aattcaggag gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt
     2041 tctttttgcc attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt
     2101 gaatgtaaat gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag
     2161 accattcctt cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc
     2221 atatcttggg aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca
     2281 gctaacatca acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc
     2341 atttggtctc catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt
     2401 gcgcgtcctt gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt
     2461 gcccggttca gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg
     2521 tcttcttgac gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga
     2581 acaagctcac gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca
     2641 cattggtaca tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca
     2701 cgtttaccta gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga
     2761 gatttcaatg ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc
     2821 aatgcatcta ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca
     2881 ttcaagtcta acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca
     2941 ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt
     3001 ggaataacag ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat
     3061 gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc
     3121 aattcttctt tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta
     3181 atcgcttctg cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga
     3241 tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact
     3301 acatatgaag caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga
     3361 cccatacgtg aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca
     3421 atatgtccca tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca
     3481 caaacgattc ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt
     3541 gtaggaccaa aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag
     3601 ttaatcgtct caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa
     3661 gccaaaccta tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat
     3721 ttcatttaat ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg
     3781 gattagtcta aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct
     3841 tccacttgtt cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt
     3901 gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc
     3961 tctgtttctt cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc
     4021 aatacgcgga atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct
     4081 tcgtatgttt tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa
     4141 gtataagccg caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca
     4201 ccaaattgag ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa
     4261 cgtgcatgca atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca
     4321 gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg
     4381 ctggccatac cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat
     4441 actggcgttg ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat
     4501 acttgcccga tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt
     4561 gttccatcag gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta
     4621 tttccgtgac gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa
     4681 acacgaacta acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt
     4741 ttcacatcgt gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact
     4801 tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt
     4861 accccttttg gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata
     4921 cggatgatac ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg
     4981 cgtgtgattt cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata
     5041 tggatagaag tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca
     5101 tagttgtacc cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg
     5161 ccttgttcca tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa
     5221 tgaacgattg gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca
     5281 tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat
     5341 tcaacgacac cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat
     5401 tccataccag taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc
     5461 atgttggcac ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct
     5521 gtcgcaacgg caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat
     5581 acttctaagt tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct
     5641 tcattcaatg ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag
     5701 tagtcgattt ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg
     5761 ataaatccga atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt
     5821 gggccttcag gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga
     5881 acttcataac cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt
     5941 ttgtgtgtca attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa
     6001 ccaaagaatt ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc
     6061 aatgtttcag tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc
     6121 ccgatacgga attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag
     6181 tggtcgatat catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa
     6241 gcaatgatgt cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc
     6301 acgtttactt cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt
     6361 ggatctgtga ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca
     6421 atgaaaggag ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg
     6481 atctcgcctg tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt
     6541 ttcaaatcta attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt
     6601 ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct
     6661 ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt
     6721 ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag
     6781 atcgtatcat cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt
     6841 gtacggtcga tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct
     6901 ccacggttag ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca
     6961 tggaaataga cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg
     7021 atgatgaatg ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct
     7081 ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag
     7141 tttgcatcat gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct
     7201 acaaattcca aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt
     7261 tctctaagtc cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt
     7321 ggtaattcca acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat
     7381 tttactacgt gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc
     7441 gatcaattgt tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc
     7501 ggcttttagg caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc
     7561 ttctactttt gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc
     7621 ttcatcagat agtttttgca acttcatata ctacacttat tttctataaa agtcaacact
     7681 tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc
     7741 aattatctga caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc
     7801 attttgtgga ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa
     7861 taacttttac tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta
     7921 aacgacagtt tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag
     7981 acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt
     8041 tttcaattta ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg
     8101 tttcccaaaa ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct
     8161 gccctgctac tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat
     8221 ggacaaaaaa caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat
     8281 aaaggattca aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc
     8341 gattatcaaa atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc
     8401 aaagaagatc ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat
     8461 gaaacaacca ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta
     8521 gtcacatcca atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc
     8581 gaaaaacaaa tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg
     8641 tataacaacg gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa
     8701 aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg
     8761 acgaaaagtc tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca
     8821 agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc
     8881 gcagttgatc aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg
     8941 ggtaaacaaa cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt
     9001 cctgtcgaaa cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta
     9061 ggcatcaatc cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa
     9121 atttgctctc tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga
     9181 ttggtgtata cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct
     9241 ttcctttagg tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag
     9301 cttgtcttct aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca
     9361 caaaattgaa aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg
     9421 tcacatcccg tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc
     9481 attgggtcga gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt
     9541 ttgctttgat cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta
     9601 ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata
     9661 tgaagatcat cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg
     9721 cgcaaaacaa cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg
     9781 cttctattat catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga
     9841 ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg
     9901 ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc
     9961 cgcagatcca gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa
    10021 tgacgtctta acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa
    10081 tcatgtcctg aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg
    10141 tggaaatggg gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga
    10201 tcagggtcaa attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc
    10261 gaaatccatc agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt
    10321 agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt
    10381 caaaccacaa gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga
    10441 aaatcgttta tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt
    10501 attgatggcc acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct
    10561 tgaccctgct acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa
    10621 tctcactgct tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact
    10681 gatcatgctg catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct
    10741 cacagtgccc caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga
    10801 tcaagtactt ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct
    10861 cttctttttt ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga
    10921 aaactatttt gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat
    10981 gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga
    11041 aaagttggca cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt
    11101 ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga
    11161 cgtactagat gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt
    11221 acaaacgatg ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg
    11281 agattctgtt tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg
    11341 acggcctttt ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga
    11401 caaacggttt gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc
    11461 cattgaatct gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga
    11521 tggaaaaaaa gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat
    11581 cagtcatatc atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc
    11641 tgatctccaa gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa
    11701 tcagctaatc agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc
    11761 gttggacttc tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa
    11821 aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca
    11881 aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac
    11941 gcaataatta tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga
    12001 attctcttca aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa
    12061 aggtattcta aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa
    12121 aacaagagag tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat
    12181 cttatagata gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg
    12241 taagttcttt tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc
    12301 acctgaccag agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg
    12361 actggtggtg tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt
    12421 aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga
    12481 ttagcttgga tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct
    12541 atcgtcatcg gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct
    12601 aaatcagcaa tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat
    12661 ttgatctgcg gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct
    12721 tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa
    12781 taatccattt attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata
    12841 cctacttata tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat
    12901 agtttggaga ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat
    12961 atgaacgttt cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga
    13021 ctaggagaaa aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa
    13081 aaaagggcaa gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg
    13141 gccgtttcag cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact
    13201 ggtaactatg gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca
    13261 actgtgaatg cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat
    13321 tttggtacag agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact
    13381 gattctttcg ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc
    13441 gttggcagta acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg
    13501 cttgatcact taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat
    13561 ttcgagcagg aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca
    13621 gctaaaacat atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact
    13681 gatttatcag ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat
    13741 acgccacttc aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt
    13801 tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca
    13861 gaagattttt cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca
    13921 atcagtgctc cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa
    13981 aaccaagacg gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg
    14041 gatcctaacg aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt
    14101 acagatacat ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat
    14161 actacctcta ccacaagtga tagcacggat acatcagctt caagcgacag tacagatact
    14221 acctctacca caagtgatag cacggacaca tcagcttcaa gtgacagtac agatactacc
    14281 tctaccacaa gtgacagcac
//
LOCUS       DANJMI010000065        14225 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000065 DANJMI010000000
VERSION     DANJMI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.065"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(2..982)
                     /locus_tag="PWU81_002484"
     CDS             complement(2..982)
                     /locus_tag="PWU81_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1111152.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(1006..2490)
                     /gene="galT"
                     /locus_tag="PWU81_002485"
     CDS             complement(1006..2490)
                     /gene="galT"
                     /locus_tag="PWU81_002485"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDL1111153.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(2502..3491)
                     /gene="galE"
                     /locus_tag="PWU81_002486"
     CDS             complement(2502..3491)
                     /gene="galE"
                     /locus_tag="PWU81_002486"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL1111154.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            3740..3907
                     /locus_tag="PWU81_002487"
     CDS             3740..3907
                     /locus_tag="PWU81_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HDL1111155.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(3969..5780)
                     /locus_tag="PWU81_002488"
     CDS             complement(3969..5780)
                     /locus_tag="PWU81_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDL1111156.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(5777..6142)
                     /locus_tag="PWU81_002489"
     CDS             complement(5777..6142)
                     /locus_tag="PWU81_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HDL1111157.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(6305..6700)
                     /locus_tag="PWU81_002490"
     CDS             complement(6305..6700)
                     /locus_tag="PWU81_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL1111158.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(6718..7680)
                     /locus_tag="PWU81_002491"
     CDS             complement(6718..7680)
                     /locus_tag="PWU81_002491"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDL1111159.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(7680..7892)
                     /locus_tag="PWU81_002492"
     CDS             complement(7680..7892)
                     /locus_tag="PWU81_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDL1111160.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(7913..8611)
                     /locus_tag="PWU81_002493"
     CDS             complement(7913..8611)
                     /locus_tag="PWU81_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDL1111161.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(8631..9173)
                     /locus_tag="PWU81_002494"
     CDS             complement(8631..9173)
                     /locus_tag="PWU81_002494"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL1111162.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(9305..10309)
                     /locus_tag="PWU81_002495"
     CDS             complement(9305..10309)
                     /locus_tag="PWU81_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL1111163.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(10306..11295)
                     /locus_tag="PWU81_002496"
     CDS             complement(10306..11295)
                     /locus_tag="PWU81_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL1111164.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(11292..12098)
                     /locus_tag="PWU81_002497"
     CDS             complement(11292..12098)
                     /locus_tag="PWU81_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1111165.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            12264..13220
                     /locus_tag="PWU81_002498"
     CDS             12264..13220
                     /locus_tag="PWU81_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1111166.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(13297..13815)
                     /locus_tag="PWU81_002499"
     CDS             complement(13297..13815)
                     /locus_tag="PWU81_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL1111167.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(13903..14052)
                     /gene="rpmG"
                     /locus_tag="PWU81_002500"
     CDS             complement(13903..14052)
                     /gene="rpmG"
                     /locus_tag="PWU81_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL1111168.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
BASE COUNT         4260 a         3091 c         2465 g         4409 t
ORIGIN      
        1 tttatttcgt tgattctcga ataatcaact ctgtttccac aataatcttt cttgaaaccg
       61 gtgctttgga attacttaat tgaagaatcg tctccactgc tagctctccc atccattcgg
      121 tataaacttt tacagtagaa agagagggcg ttacatattt cgctacactg acatcattga
      181 aacccatcac agaaatatct tctggtacca ctaaaccttt ttcttgaatc gctttcagtg
      241 cgccaatcgc taatgcatca ttagctgcaa aaagcgcatc aggaacatgc ggattcgttt
      301 ttaaatactg cttcactgct tgatatcctg actcgacaga aaaatctgct tcgataattg
      361 gaccttcgtc gtagcctttt tctgccaaaa cttgttgaaa tgccataatt ctgggatctt
      421 ttagccgctg atggtttttc ttcgtatatt ctgttccagc aagcatagcg atatgttgcg
      481 ctttatggga gacaaaatgt tcaatgactt tttgaacact tgacttaaag tcgacaacta
      541 gagagtcgat tccttgagaa gtgccgtcag aatctaccaa aagcaaggca ttttgggatc
      601 ctttcatttc taataactga gcttcgtcga attttcctag tgcgatcgtt ccatctgtcg
      661 gaagatcact aatggaatcc cacgtttctt tcaataaagt aacgtttgct tcttctgcct
      721 ttttttcgat tcctagacga atagataaat aataaagatc ttcaagctct tccgtatcgt
      781 cgtaccactg gatcaggcga agcgtttgct tagtatttgc tgcattttta cgatgtttcg
      841 tataatttaa gcgttctgct gcttcaaaaa ttttttgctt tgtggctaag ccaacggaaa
      901 gatcaggatc atagttgaga actcttgaaa cagtagcagg agaaactcct gccaactgcg
      961 caatgtcttt aattgttgcc atttgtctct cctccttttt tacgtttata aaattgcagt
     1021 aaatcgttca aacgctgcct gtcctttttc atctcgcttg aatactccag cgtcttccag
     1081 tacacgtgca aaaacctgac ctacagattt ttgtacgaca tcggtgaccg tatctttcgt
     1141 caaatcagga tgttcttttt ttaattgttc tgcccacgga cgatgatatt cagccatctg
     1201 gttttcttct cctaataaga aatgctctac ttctactagt tctggtttca aacgaggtgg
     1261 taaaacagca agccccatta cttcgatcaa cccaatgttc tcctttttga tatgttggac
     1321 atctttatgt ggatgaaaaa tcccatcagg atgttcttct gaaacattgt tgtctcgaag
     1381 gacaaggtcc aactcaaagt aattgtccct tcttcgtgca attggcgtaa tcgtatggtg
     1441 tggtgtacca tctgctgaaa aagctctgat agatactgtt tcatccgaat atgctttcca
     1501 cttttccaat actgagttag ctgcttcaat caacaatttt ttctctgttg attgcaaacg
     1561 gatcacagac atcggccatt tcacgactcc tgcgatgact tgcggataat ctgctagttt
     1621 gataagtttt tctatcggtg cttttgccat aggaaattca tgacgtcctg cctgataatg
     1681 atcatgagaa agaatcgatc ctccaacaat cggcaagtca gcattcgaac caacaaagta
     1741 atgaggcaag acttcagtaa tccgaagcag tctagcaaat gtttcctttg tgattttcat
     1801 cgggcggtgt tgttctgata aaatgatcga atgttcattg taataagcat aaggagaata
     1861 ttggaatccc cagctttcac cgtctagatt cattcgaata atccggtgat tggtgcgggc
     1921 tggatagttc aagcgacctc tatatccttc gttttccatg caaagcatgc aggtaggata
     1981 gtctactttt accgcctcgc gttctgctgc gatttgtttt ggatcttttt ctggcttaga
     2041 taaattgatt gtgatttcca gttcaccata ctccgactct gccggaaatt tgatgttttt
     2101 tgcgatggct cttgttttga tgtagtcatt tttcttactt aaattaaaga aatactcagt
     2161 tgcttcctct gggcttttcg aatagtgctg tgcaaagaat gcattgacga ctgaaggtgg
     2221 cggtgtgaga aaatccatta attgcgcttc aaaaatctct tgttctgctg gcaattctga
     2281 aagaacacca ttttctcttg ccttagcgac aagctgatcc aataagtcta cagattcttc
     2341 cttaactggt cttacttcaa tgctttctag agcatcttct cctaccatcc ctaaaagacg
     2401 attctgtaaa tataagcggt ccatttccat ccatccgcca gcttcgatcg caagtgtggc
     2461 aaaatcacaa atcgtttgac tgattgacat atctcattcc cctattcttc ataaccattt
     2521 ggatggctga catgccaatc ccatgctgtt ttgataattg ctttgatatc tgtgtattca
     2581 ggtttccaac ccagaacctc tctcgctttt tcacttgaag cgactaatcg gctaggatct
     2641 ccagctcttc ttggtgctat ttctgctgga atttcttttc ctgtcacttc tctagctgct
     2701 tctagcattt ccttgactga gtatcctttg ctgctgccta aattgaagaa gttgctttca
     2761 tttccttctt tcaagtattc caaagctaaa atatgtgcag caatcaaatc ttctacttga
     2821 acataatctc tgatacatgt tccatctggt gtatcataat cgtctccata aacagccaat
     2881 gcttttcttt gaccaagagc tacttgtaaa atgattggga caagatgcgt ttctggtgta
     2941 tgatcttctc cgatagatgc atctgctttt gctccggcta cattgaagta gcgcaaagcg
     3001 acatagcgca tgccatatgc ttggtcacac cacttcatca ttttttccat catcaattta
     3061 ctttcaccat acggattttt aggattagct ggtgtatctt ctgtgattgg agactctttt
     3121 ggttctccgt aagttgctgc agtagaagag aacacgattt tattcacatt gaactcgtgc
     3181 atgacttcaa gtaagatttg cataccgtat acgttgttgt tgaaatacat caatggtttt
     3241 tcgacagatt ctcctactaa agagcttgca gcaaaatgga ttactccttc aatcggttcc
     3301 ttttcaaata cggaacgtag aaattcttta tcccggatgt ctccttcata aaaatgagca
     3361 tctggatgta ctgcttgttt gtgacctgtg agcaaattat ctaccacagc tacttgatac
     3421 cccttttgaa ctagctggtc tacagcgtgc gaaccgatat aaccggctcc gcctagtact
     3481 aaaattgtca taaaaaacac tcctttgatt agtaaaattg tgtttgttga ttatatataa
     3541 tttaaaaaaa cgaagtttac ttttatggtg aaaacaaaca aagtaaaatt tcgtaaattc
     3601 atatggtaaa tttataataa aacatttagt aaacattttc aaggattttt tgcaaaggtt
     3661 tacaaacaaa cgtaaaacta gccttttttt ctcttttgtt ccataataga tataacaaat
     3721 aataaatgag gtggattcaa tgaaaaaatt tgtatctgga atattagtag gaagtctggc
     3781 aacagccgct gccgttgctg gtttggtcgc ttctgtgaaa aaaacagtga tcgatccaat
     3841 tgacgaaaaa gaagcaatga tcgaagaaaa ccgtaaaaaa gcaatgcgca aacgcgtttc
     3901 tcgttaataa ttatttaaaa acacgcatga agacttgtga aagaacagat aagtcatcat
     3961 gcgtattttt atttgtcagt caatataagt tgtgctattt tatctgcctg tctcagattc
     4021 catggatctg tataaggtct agctactgct gcagttagcc aatccacacc ttcaacaggt
     4081 attccatagc aataaaagtc agactggatt tcccctgaag catcaatcac gcgattcgta
     4141 cctcgctcaa catctatagc ttctgaatga taacttcctg tattatctga aaatgaaaaa
     4201 gggcgaacaa gtttgttttc cttcaaattt tttatgacag gattacttga acgagaaaag
     4261 cttgtttgtg gcaatcgagc ttcaatcaaa tagcgactgc tgatttcttt atcttgtaga
     4321 gaaataacat agcggttgtg cttttgctga atatcaaatg gcggttcgaa caaatgaaaa
     4381 atacccgcat ctatcagtgc tgctaattct ttcgtccgaa tgactggcgg accgatcgtt
     4441 aaaaagctat ttagatgagt aaaccatccc catagtattt ctttgtattc ctttgcagaa
     4501 aaaacgtgcc attcaatagc ctgccggatt ggatcacgcc agtcttttaa tgcatcaaat
     4561 gttgaagcaa atgctcctgt gcaattgcct ttttccgctt cttttatctg cccttcaata
     4621 aatgctcggc tagcttctgc aaaggactca tcctgatagg taagaggaat ttctaaatac
     4681 gaccaatccc atctataagg gattagtgct ggatgctttc cgagacactc ctctgaagag
     4741 agggagagaa aatcatgctc aaaatttttc tttggaatcg gcaaatgata ttcttcgatg
     4801 attttcttgt aataaaacaa ctcaacgtct tttttcagat agtcaaaaaa tgtttcgcca
     4861 gtcaaaaatc ccgactgatg ccattgctcc aggttttctt ttgttagtaa ccgtggccat
     4921 gccattgatc cgccttgctt ttggtttctt cctcgagggt aataagggag cccccttctt
     4981 gatccactgt atacaatcgg ttccttgcct gatggatgat agacaagttt tcctttttcg
     5041 ttctcgaagc gcccttctct tgatactgta aacaatccga cataatcaaa aaaacttaag
     5101 cctagacctc ttaaaaagac attttcttta ggcggtatct tcgaaagatc gacatctgca
     5161 ggattagaag gaagctgata cagtaaatca tttttctttg cgtagtctac ccacactctt
     5221 tcttcttctg tctcatcatt ttcccagtga ccgcttgcta aaacaagctg atcgatagaa
     5281 tagctttgct gatctgtctg taaaagataa ccatcttttc ttttgctgac tttttttact
     5341 aaagaataaa caaactgaat agaagctgga aattcttctt gcaactttga gaaaaaccag
     5401 cgttgataga gtccgtataa acatcgagta gattgctgat ctttttccag acgattgatt
     5461 tcttctataa ttagttgctt atttttggtt accgtctgtt cgatataatc tgtagcaggt
     5521 cctttactcc attgaaacaa gttgggtccc tgtgttacca atcctccact ggacaatgtt
     5581 tcatctgtaa aaagcgtcac ttgttgagaa acagaattca ttaataactc cggtggctga
     5641 ttcaaccgcc aaactcgacc tccaggacca ttgggatcga aaagaaaaag ttcaagagaa
     5701 tgtctgtcct gattattatg gagaatcctt tctgctatac ttaatcctct aggtccggca
     5761 ccaatgatcc ctattttcat ttgcatgcct ccatttcgat aaattttttc aataaaacag
     5821 catggttctg atgttcattt tttgcaccat aaacaaaagt aatgactgga tgttctttca
     5881 taagttgact gagtttaaaa atagcttgct tcttttcttc ttcgaaaatc aattcttgct
     5941 ggtatttctt tgcaaatgaa gcaaatcgtt ctggtacatg gtcaaaagtt ttgcgtgctt
     6001 cattactagg tgcaatttct ttcaaccaca gatctaactg cgctttttct tttgaaattc
     6061 ctcgtggcca gaacctatcc acgagtattc tatagccatc ctctttttca gcatcaaggt
     6121 aaacacgttt taatttgatc atagttgttt ccttctttct gccttttcgc ttagcttata
     6181 tatgtagata atagcacaaa ataaaaaaaa tgaacggcac aactatccat tgcgccgttc
     6241 atgtgatctt ggtcaaaatc taatgattga ttcggttttt aaagctgtca tacaatcatc
     6301 tgttttatac ttttttactt ttgatttttc cagaccatcc gtcatatcca cccttaagta
     6361 aataaatctc ttgataacct gctttacgta atttatttgc tgtacggatg ctcaagcttt
     6421 tcttttgatc gtaaatatag acaggctggt cttttcgaat agatcctaat gaatctttca
     6481 aaacagtgta agggatgttt cttgcaccta aaatatgccc agcatcaaac acatcttttt
     6541 cgcgaacatc gattacttgt gcttttctca ttccttctcg aaattcttct tctgtcaaag
     6601 tcgttgctga acgcttggcc attactcgta gatagacttc ccaaaagatg attgcaagca
     6661 caatcaatag caaaatgata ttaattaccc ataaaaccat gtaatgcctc cgttttttta
     6721 agcaaatttc aaggctaatg aggcagcacc aatgacacca gcttcattac caagctctgc
     6781 taatttgatt tttgttgatt ctcttacttg cgggaaagta aattctttaa aatatttctc
     6841 tacacggcta cgtaaaaatt ctcctgctgc cgatactcct ccacctaaca caatacttga
     6901 cggattcaat gtattcccca agttggcgca agctaaacct aagtagaagc atacgcgatc
     6961 aacaaccatc aatgcaaacg gatcgtcttc ttgagccatt tcaaagatat ctttacttgt
     7021 tacttcttca ccgttgtcta acattgcttt cagttttgaa tctcctgcgt attcttctgc
     7081 caagtaacga cctagacgta ccacaccagt agcgctagac acagtttcta aacatccgcg
     7141 tttaccgcaa gtacattcga acccttcagg atccacagtg atatggccga tctctccgcc
     7201 tgctccgctc acgccgtgaa tcaaatttcc gccagcaacg ataccaccgc caacacctgt
     7261 tcctagagta acgaatacaa cgtctggatc gttttctcca gcacctttcc atcgttcacc
     7321 taaagcagca acattcgcat cattatcgat agcgaatttg attcctgttc ctgcctcgat
     7381 cagttttttc acaaattgaa cttctgtcca gtttaagtta tacgcaccaa taaccgttcc
     7441 agcttcgctg tccacgcttc ctggggtacc catgccgatt ccgataaagt cttcggcttt
     7501 catattatat aaattcaaac gatggttgat tgattcaatg atttcaggaa cgatatgctt
     7561 accatcatcc aaaatattgg tatcgatgct ccatttttgt tgaatctctc cttctggtgt
     7621 taaaattgca aattttgctg ttgttccacc taaatcaatc ccaataatct tcttgtccat
     7681 tacaaatttc ctttctttcg gctttcttca attcggtgct ctctttttaa tatgcgccac
     7741 gctgtcatgt atgtatcatg atcaatcaaa cggctatcat acagattacg caattcgata
     7801 gtcattaatt caatgtcata gatgcgtgca cccacataga taaagatgcc aaattttttg
     7861 aacagctgct gtacatcata taagctctcc atctttaagc acttccttca tattatacaa
     7921 gtaaaccata tttttttaaa cccagtaaaa tacatattac aaaaagaaat acaaaaacta
     7981 gtgtagaaag aatcctttgg tgtatcgaat agcttgtctg ttgtttcggt agacccaaaa
     8041 cattccctaa cactaatccg ccaattagcc caccgacatg tccccaaaca tctacagagg
     8101 tatccagaag accaaaaaca aatgtcaaca caataaataa aagaaaatgt ctgactagct
     8161 gctggatcgc tgggttatgt ttaaaatgaa accctaagat aaacaacgca ccgaacaaac
     8221 cgaatatcga agtactcgca ccaccagaca gaacattcgc ttcgttaaaa gcaaagcttg
     8281 ctgtatttcc cattatcccg ctgaacatat aaatcaagaa aaaacgccaa tggccataga
     8341 ttgcctcgat ttgctgcccc ataaaataca gtatcagtga gttcattacg aaatgcatca
     8401 gtccaaaatg gatgaagatt ggtgtcacta aacgccacca ttcattgaag tgaacaagat
     8461 aaggaccata cattccggca taataaggga tctcaagtcg cggaaataat tccatcatga
     8521 ggaagaccgc tgtctgtatc cctaaaaaag cataggtcca taacggtcgt cttaaccatg
     8581 cacgcaattt tatttgctgt tgataattca tgaaaacctc ccatttcatt ttagtcaata
     8641 aacaattgtt ggatcttctg atcaaagctc tccggttgcc attgttcatt caattgttca
     8701 cgaaaaacaa ggctacatgt ttttttatga aaattattta aataccggtc gtaataacca
     8761 ccgccaaaac cgattcgata accggctcta ttgtagataa tacctggtac aatgaggaga
     8821 tcgatcatct ccggcaaata gagcttctct gattgtggct caagaacacc aaaactcgaa
     8881 cgtacaagaa cggactgttc atccatttca taaaacgcca tctgacgttt cggcaaagtt
     8941 ttaggtacaa ctattttctt ttgttctttt tctgctcgtt ggataaccgg ctgtgtatcc
     9001 agttcaaatt cattcgataa agtcgtccct atcacttttg ctgacttcca gtactcgctg
     9061 gcgaatagct gtttatagat tcgctgttct ttttcctttt tcttgccact tttggccaga
     9121 tttttcagct tttcgattcc tttccggcgt aatcttactt tcaattggtc caaaaactca
     9181 cccttccttc tctcataaaa tggtatagga aaaaaagaaa aatagcaatc gaactttcaa
     9241 ataaatgcat aaatagaata aaaagaagac ctagaaatga ttggccttcc ttttttaata
     9301 aatttcaact gttcagcgcg tgtttttgtc tgataaaaag tgcatacaga aaatatggcg
     9361 caccaaaaag ggcaatcaaa attcctgtag gtacttcccc acttggtaga atcactctag
     9421 caaccatatc tgcactaagc agaaaaatgg aaccgaacaa accgctcaga acaaaggtcc
     9481 agttattttc tttacgtacg actagcttcg cagcatgagg agctagtagt cctacaaaag
     9541 ataaactacc tgtcaccgct acactaccgc ttgccaaagc aacagctaac aacaaaagga
     9601 ttttttgact tttagccacg ttaaggccta atccttgcgc gcgttcttga ccaaatgata
     9661 gcaatccaat cctctgtgca taaagtcctc ctagcggaat taagataacc gcccatggaa
     9721 gcaatgccca aacataaggc cagctcgcac cccagattga tcccgctaac catgcattca
     9781 caaatccata attttcctta gataattgga tcgtgcatag caatgttaat gcagaaaggc
     9841 cggcattaac ggcaataccg gacaataaga ttcgattcat acccaaaaag ccatttcgat
     9901 cataggccgc aagatagaca cagcaagcag ctaaaacacc acctagaaag gctacaacta
     9961 gcaatagaaa cggcagcgaa tagtgcgaaa agaaacctaa ataaagcatc actaaaaatc
    10021 ctgctccggc attaatgccc aatatactag aatctgccaa ttcatttctt gttactcctt
    10081 gcaagatata accagaaacg ccaagacaag ctcctgctaa taaactgatc agtaatcgag
    10141 gaactcgaaa ttctctaaca atcagcgcca aagaagatga ttctttccct atgaacgtgg
    10201 caaaaagctg atccaggtaa ataaaaggcg taccaatcat taaactacct atagacagga
    10261 tgacaagtac aaaaagcaag aaatagaacg tccaatatat ttttttcacc gttgttccct
    10321 ccttacttgt atcagtaaaa acggaacgcc aatcaatgca atgactaatc caaaaggtgt
    10381 ttcaaaaggg ggattgatca tcctcgctgt aaaatccgcc aacataacca atccaccgcc
    10441 aactaaaaaa gagacaggta gaacataacg atagtccgtt ccaaccattc cgcgaacaac
    10501 tggcggaacc aacagcccaa taaatgaaac aggtccgact aacgaaacgg caatggctgc
    10561 taacactgca actagacaga aaacaatcaa ccgcgttcgt ttaggatgac ttccaagagc
    10621 taatgcatgt gtttcactca aatgaatcaa agatattgct tttcctaatg aaaaagaaac
    10681 caaaacagca agtatatata aaggaaacaa tactgctaat tgtttccatg tgatattggc
    10741 agctccgcct acaaaccaaa aagcaagatc ctgctgcaga ttaaagaatt gggtaagtgc
    10801 ttggctgata gcagtaaaaa aagaactaat agctacgcca gctaaaatca gctgtaaagg
    10861 attcatgccc attttcgaaa actgcaaaaa accaaatacg agcaaagtta cagcaaaaga
    10921 acctgccaat gacattagaa acacagaaaa atcagaagct ttaggccaaa aagcaaaact
    10981 aagggccatt gccaacgaag cacccgcgtt gattcctagt aagcctgaat ctgctaaagg
    11041 atttcttgtc actccttgca tcaatgcacc gctcaatgca aaacaactcc ctacaagaaa
    11101 tgctcccgcc acacgaggca aacgaatatt tcgtatgatt tggtgacttt gttttgatct
    11161 atcaaaatca aacaacgcct gccaaaaatc tgcttttccc acagtcgctg cgccatttga
    11221 aagagcaaca aaaaaaataa tcaaaagaac aaaacagacc aaaagcagga gtctattttt
    11281 cttcatagtc ctcataattg atcctcctgc tcttttggca acaaatcata ggttagaaaa
    11341 actggtttat ttgttcttgg aagcgtgaca atcgttgcat caatctcaaa tatttttttc
    11401 atacactctt ttgtgatcac ttcttcaggt gtaccttctg tgatcaactt accgtttttc
    11461 attgctaata aataatctga aaaacgtgaa gcatgattaa tgtcatgaat cgacatcaag
    11521 atcgttgttt gtttcatttg attgatttca gtcaataact gtaggatctc taattggtgt
    11581 gccggatcta aaaatgttgt cggttcatcc aagatcaaga tatctgtttc ctgagcaagc
    11641 gccatagcga tccagacacg ctgggtttgt ccaccagaca gcgttgcgat atcccgcata
    11701 cgaaatccag ctagacctgt agcatccaac gcccactcga ttgcttcatg atcttgcgtg
    11761 tccatgccac tgaacagttt ttgataaggg tatcgtccgt aagatactac ttcttctact
    11821 gtcatacctg tagggtgttc actagtttgt gctaataatg taattttttt tgcaagttct
    11881 tttgaactgt atgtagagat atcagagtta ttgacaatga tctttcctga tttcacaggt
    11941 agtatacggg taaaactttt caataaagta gattttccac ttccatttgg cccaatcaaa
    12001 ctagtgattt tcccttcagg aatcgtaaca gacatgtttt caatgatttt tttgttttcg
    12061 tagccggctg caatgtcagc tgctcttaat ctcgtcattt cacccatcct ttcgctgaaa
    12121 atcattctca attatgacgc tgtttcaaca tttcgtcaag cgctactttc atatctatag
    12181 ccaaatgaag cggttgacag cgttcgaaat atcaggaata ataaccaatg agaaagattc
    12241 tcaattatgt taggaggaaa gaaatgaaat taaaaacaaa agtattcaca ttatctagca
    12301 tattgctgct ggctggagta ctgagcgctt gctccccctc ttcagataaa gaaacagcta
    12361 caactgccaa ttcgacgact tcatccaata gtactcatac agtcacagat acacttggac
    12421 ataaagtaga gattccaaac gcgcccaaac ggatcatcgg cagttactta gaagactatc
    12481 tgattgcact aggggaaaaa cctatcgccc agtggactgt tggcagtggt tccattcaag
    12541 attatctgca agatgattta aaggatgtcc caacgatttc ttatgacctt ccttatgaga
    12601 aagtgttaag ctttgagcct gatttgctgt tgatttcttc ttccgctact gtcgaaggcg
    12661 ggaaatacga acaatatagc aaaatcgcgc caacttatgt tgtaaaaaac ggaacagatg
    12721 tcacatggga agaacaacta aaagatgtcg gcaaagcttt ggcaaaagaa aaagaggcag
    12781 aaaaaatcat ttccgattat caaaaatcca tcaagaaaac tcgagaagaa ctagcagata
    12841 aaatcgagaa caagacagct gcagtgttgt gggtgaccaa caactcagcc tttatggtca
    12901 gcaaaaatcg gtcaagtgga agaatcgtat acgacgatct ggaatttggt gttcctgatc
    12961 tagtggaaga ggtctccaaa aaagccactt ctgactggtc tgctgtttcc tctgaaaagc
    13021 tagctgaatt agatgctgac tacatcatcc ttgtgaacag tgacaaagat gctgccatgt
    13081 tcaatgaacc aagctggaag aatcttaaag cagtgacaga caatcatgta ctgcaattcg
    13141 gacctgaatc tagttggcta tataacggac ctattgccag tcaggaaatg gtcaatgata
    13201 tcaaagaaaa actaaattga ttttaactaa aaaaagaatc ttcacgatta gtcgtgagat
    13261 gctttttttc ttattaacaa actagacgca tagattttat tcttcttttg aagcaaccct
    13321 attttttact ctttcccatt tttgaaaacg atgggcatat agattttctt gatccgttat
    13381 cccctcatac tcctctataa gggcgaagtc tgaaaaatca atattcccaa tatacgtatc
    13441 gccttcaaac gtgctatcga tcaatgttcg ccagatccga ccagtgtaag gaagaagttc
    13501 ttgaaaaatc cgactaccac ccgaaacata gatatcttct gagatcgttt gactatacga
    13561 caaaagttca tcgatcgaat gcattacttc cgcattttct ttcgtgcgaa aatttggttg
    13621 cgttgtcaac acgatgatat ggcgataagg aagtgaaaga tcccccattc cttcataggt
    13681 ctttcttccc atcactaaaa ttcgatccat tgtatgttcg cgaaagaaac gcatatcatt
    13741 tggcaatcgc catggtaaca aaccattttt gccgatcaga ccatttttat cttgtgccca
    13801 catggaaata aacatattat ccgctcctta tgattttaga tgttttgaga taaaaaaagt
    13861 ctcaattctt tgaccagcaa ggaattgaga actttaaaaa cattattttg tttcacggtg
    13921 caaagtaact ttgcgttcgc gtgggcaata tttttgtttt tccaaacgat caggattgtt
    13981 acgtttgttt ttgcttgtta gatagttacg ttctttacaa gaagtacact ctaaagtgat
    14041 gtttacgcgc atgtttttcc ctcccatttt tacaagaatc ctctaagatg actctcagac
    14101 ttgattatct taccatgatt ttttctagat tgctagtgtt tttcacgatt ttgttaagta
    14161 aaactacttg acgataaata caacggtttt acttctcaaa caaaatcttt ttctttttgt
    14221 ataaa
//
LOCUS       DANJMI010000066        14130 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000066 DANJMI010000000
VERSION     DANJMI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.066"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(<1..244)
                     /locus_tag="PWU81_002501"
     CDS             complement(<1..244)
                     /locus_tag="PWU81_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL1111169.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
     gene            complement(237..1829)
                     /locus_tag="PWU81_002502"
     CDS             complement(237..1829)
                     /locus_tag="PWU81_002502"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL1111170.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(2339..3064)
                     /locus_tag="PWU81_002503"
     CDS             complement(2339..3064)
                     /locus_tag="PWU81_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111171.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(3218..3544)
                     /locus_tag="PWU81_002504"
     CDS             complement(3218..3544)
                     /locus_tag="PWU81_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111172.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(3581..3946)
                     /locus_tag="PWU81_002505"
     CDS             complement(3581..3946)
                     /locus_tag="PWU81_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDL1111173.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(3983..4198)
                     /locus_tag="PWU81_002506"
     CDS             complement(3983..4198)
                     /locus_tag="PWU81_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1111174.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            4327..4662
                     /locus_tag="PWU81_002507"
     CDS             4327..4662
                     /locus_tag="PWU81_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1111175.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(4738..6735)
                     /locus_tag="PWU81_002508"
     CDS             complement(4738..6735)
                     /locus_tag="PWU81_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111176.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(6833..7108)
                     /locus_tag="PWU81_002509"
     CDS             complement(6833..7108)
                     /locus_tag="PWU81_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1111177.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            7340..8074
                     /locus_tag="PWU81_002510"
     CDS             7340..8074
                     /locus_tag="PWU81_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1111178.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            8113..9255
                     /locus_tag="PWU81_002511"
     CDS             8113..9255
                     /locus_tag="PWU81_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1111179.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(9883..10368)
                     /locus_tag="PWU81_002512"
     CDS             complement(9883..10368)
                     /locus_tag="PWU81_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HDL1111180.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(10370..11134)
                     /locus_tag="PWU81_002513"
                     /pseudo
     CDS             complement(10370..11134)
                     /locus_tag="PWU81_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11304..12497)
                     /locus_tag="PWU81_002514"
     CDS             complement(11304..12497)
                     /locus_tag="PWU81_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1111181.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(12581..12781)
                     /locus_tag="PWU81_002515"
     CDS             complement(12581..12781)
                     /locus_tag="PWU81_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDL1111182.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(13205..13438)
                     /locus_tag="PWU81_002516"
     CDS             complement(13205..13438)
                     /locus_tag="PWU81_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1111183.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(13445..13876)
                     /locus_tag="PWU81_002517"
     CDS             complement(13445..13876)
                     /locus_tag="PWU81_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL1111184.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         4295 a         2896 c         2270 g         4669 t
ORIGIN      
        1 tgattaattc attatatttt ttttcagtaa ttttatatct tgtttcgtaa ttatcataaa
       61 tagcattttg ttgttcatag acaacaaaat tgctattaga tacaaagaat tctttcttta
      121 aggcactccc aaatggtccc ctccttactc catctttttt atcaagtaag cattcaaact
      181 tacgctgttc ccaagcttcc gtaaatcctg ggaaacgaat ttctggttgt atatcattac
      241 tcatgtttga acacctccag cgcaccttgt aaccacgctt cattttcagg tgttgtttgc
      301 agggaagaga tagcttctaa aagttctttt tctagcactt gctgttcttg tcgtatcttc
      361 ttgatgtcat ttccgacatg aaccatatca attggttctt cttcttcaaa cgtatctaca
      421 tagcgtggaa tatttaagtt atagtcattt tctttgattt catcaaagtt tgccaaatga
      481 gcgtatttct cgacatcttt tctttcagca taggtttcga ggattttttg aatgttttct
      541 tccgataatt tattctgatt ttttcctttg acaaattctc gactagcgtc aatgaataat
      601 acgtctcgag tttggcggtt ctttttcaat acaatcaccg tcgttggaat cgatgtcccg
      661 aagaacaagt tcgcaggcat accgatcaca gcataaatac tgccgtcttc tagtaatttt
      721 tgacgaatga ccccttctgc agcgccacgg aataaaactc cgtgtggcaa gacgatggcc
      781 atagtccctg tttccttcaa atgatagaaa ccatgaagaa gaaacgcaaa atctgctttg
      841 gattttggcg ctaattttcc ataacgatta aaccgagaat cgtctaagaa ggtcgtatct
      901 gctgaccaat tagccgaata aggtgggttc attactactg catcaaatgt gtaaggttca
      961 tctgttggcc aatccttatt caacgtatcc ccattgcgga gattcatttc ttcggcatct
     1021 accccatgta aaatcaagtt catttttgct agattgtatg tcgttgtatt taattcttgc
     1081 ccatgatatt taacgttatc tgggtgagtt aagtagttac gcacatttaa catcaatgaa
     1141 cctgaaccca tggttggatc aaaaacacta aagaatcgtc gttctttttg atccaacgtc
     1201 acgatttgtg ccatcatatc agaaaccata tgaggcgtgt aaaattctcc ggctttcttc
     1261 cctgcttctg aagcaaattg actgatcaga aattcataag catccccgat cacgtcccca
     1321 tcgtgaccta aaacttccac gtcattcaat ttcttaatga cttcggtaat cgtcacattt
     1381 cgttgttgct catctgttcc tagttttttt gattggagat ccacatcatc aaataaccca
     1441 ttaaattgat tgtaggtaga tgccaattgc acaaaggctt tgtttagatc attcaattgg
     1501 aacgttgctt gttttgcttg atccgctaag acgttgaaaa gatactctgg cgaaatcgca
     1561 taccccaaaa tatcaacgat tgttgcaatc agatcttctt ttgattcttc atctgaaagt
     1621 aattcgcgat acaacatcgt ttgtttgctt accgtatcat attcctctaa ggattcatct
     1681 gctaaaagaa ccacttgttc caacaaacgg tcggataaat atttatagaa aatcagtccc
     1741 aataaataat ttttatattc tgacgcatcc attttgctac ggagattatc tgccgcacta
     1801 aataattttt gatttaattc tgcacccaaa aagatacact cctatctctt atttaaagtt
     1861 gctatatacc ctcttatttt agcatactcc cttttagaaa aagcaaaaaa aaaaagaaag
     1921 aaacttattc ttgctaataa actacgcgaa aggagtctaa cgttgtttga tataacttag
     1981 actgatcgtt aacttatttt ttatatttct acatagttat ctttagaacc atttcagata
     2041 catatgaaac aattacatat gattttttca taaaaaaaca gtactgtcta gtagtataaa
     2101 atatacaaat ttcttaaaaa ttttttcact aaaatcacta cctgaaacag tctaaaacac
     2161 ttatatatca acgtttaatg aaactatctc cgtttcattt cagatacaaa taaatatagt
     2221 cgggctccaa atccgaaaaa aaataccccc agtagcttat cctaagctac tggagatgta
     2281 taagtacggt catataacaa cggggtaggg taaataaatg aaaaagatgg aagaatcatt
     2341 attcaataac ttttttaaat acttcgttgt aatcagttac ctgtccgttc ttaaatacca
     2401 cagaataatc ttttttccca ttttcaagta accgaagtgg aatatcttta ccaactttct
     2461 ctgtcactgc attactgata cctgttagat tagctaataa atctgataaa gattgagcat
     2521 ctgtagaaat ctctgtatca gtatttatcg tgattccttt atcttcattt acaagtttcc
     2581 aagtaggtgg tatagctgtg tatgtttcta caaatgaatt aagtttttca ttaagtgact
     2641 tctcttttat atttccatct aaaaactctg tatatatggc ttgagctata ctaaaacaaa
     2701 gctggccttg ttctaataat tttacatcat tagttagtaa agctgtgttc acgttatcaa
     2761 tcgcttcaag aagagtatct ttggtgtccc taacagcttt aggtgtgcct tctggaattt
     2821 ccctacttga tacctcgtcc attttatctt ccataaaccc cttaaaatca tatctttctc
     2881 ccaaatccat agaagttttt gagaaagctt tcatttctga gtctatttct tcaataatat
     2941 ttaacatgtc tgatacatat tgtttaggct gattcgtttt taatctatat gtggtaaata
     3001 tctcaattcc tccaattatc aataaaattg ccaaacaaat ccataataat tttttctttt
     3061 tcatttttcc tcctatacac tgctgttata acagtgacta atgtaaagat tttcccagca
     3121 atcatcagac tattatttac agggaatcaa aaaaactcct tagtaagctt acacctttag
     3181 gagttcctgt ttgaacggta tgcataatac atttaaatca atttaacatt aacacctcag
     3241 tatatggtat ctctacatat acacggtctt tttttggata gtactcgaca tttatattga
     3301 tagcacttcc attgtctaac gtagctttag cccggtacgt gttatcacca ttatcatgag
     3361 tgatcgtcac atcatcacat gtccgattta agttatactg ttcttgtaaa atctgagtta
     3421 ccaatggtac agatgacttc tctagtccag atgaacccgt cattcttgaa aaaattatac
     3481 ctataatagc caatacaacg atacctataa tgacaaactt cttattcata tttactttat
     3541 tcaacttttt atctcctatc tccaaaatga ttttctctca tcagtaactc attgacatcc
     3601 ccaatagtac accaaagatg gctccagcta cccctgctat catcaaaatc gtacccattt
     3661 gttcatcttt ctctcgatat aagtaaccca taataacagc aacagcacca aataccacag
     3721 gaataaatgc taatgatact accatagaaa tccaaccaat aatcaaatac agcttccctg
     3781 tctcttgtcc atgtacgatt tgttccttca tttcattaat tgtctgctca gtacttttct
     3841 ttactgtgtc tgttccggtt gtctcatctg tttcttgtaa tgctccgcaa aactggcaat
     3901 acttactttc atttgttatt tctttaccac attttctaca atacatgtct tctcctccta
     3961 aaatcattca attgcttatt agctaactgt ttactttatt aaatacatca gaaaacagct
     4021 catttattgg tacacttaat atctcagaaa gcattttcat ttgatagggt ttaggaatcg
     4081 ttctccctgt ttgccacgaa cttacacatt gctgtgtaac attcattttt ttggctaact
     4141 cagtttggga aagtcctttt tcttttctgt atcggctgag attacttact tttttcatga
     4201 ttctcccctc ctgttttatt atatacaaca atatgatgta tttcaactca taaaatatat
     4261 aaatacttca aatgtcatta caaatacaac aacttgttgt actctactga gaaggggggt
     4321 gtattaatga aacagaagca atatgcaaag gtcattagag aaaagcgaaa agcactcggg
     4381 tggacacaaa aagaattagc taaaaaaatc ttctctacac aacaggcagt agcaagatgg
     4441 gaaaattctg taacagaacc taacctcgat agcttaacag ctctttctag agccctagga
     4501 acccctgtaa gccattttct agacaatgtt gacatagatt atgaggaaga gtttttggct
     4561 ctctatcgct ctcttagtac tgaagatgca attcgaacaa ttgactatat gaagctatta
     4621 aaaaggcaag aaaatgaacg caatcagcta ttgaaagatt gaaaaataaa taacccaccg
     4681 aactttaacg gtgggtgtat tgagatgaaa taagattaag agctatggat aacctgtcta
     4741 gtttaatata attgggtctc ttctattcct tactctccgt ccatcatctc ttgttacact
     4801 tgtaaattga taaataggaa ctaattcaac tcctgataat atcaattctt ttattacttc
     4861 atctatccct gcccctttcg ctagatatac taaaaaacta ctgcttgctt tcacattgta
     4921 tagtttctta atatccttag aagctcgttc aagagtgaat ccttgtttaa caaagtagcc
     4981 tctgttttct attaaagcat ttttcacgct gttatccctg acatttcgta ggcgttcaca
     5041 aacagtgtta ataagttctt tttctgtcat tagatatgtc ccttgccacg ataagccctg
     5101 taaaacttca tgtgcttcat ctaaaatcaa ttgtttttcc ttgctaagtt ctgatcggga
     5161 attttgagga aatactcttt tagcctcttt ttcgttaaaa catatcttaa cccattcata
     5221 agaaaaatat tgatatgtaa aatgaaattt ttccagaagt ggaaggatat cttcattcaa
     5281 tttttgaatc acagaagttg cattaaaaac gtctaatcga tagacattgc ttctcttatg
     5341 tttaaagttt cctaacgctt tctcttcctt taacctattt aggtattcga gcatcttcaa
     5401 aggaacatct tcatcatcta atttttttat caatcctata aaagtcataa gtgatagtaa
     5461 cttattacat ttatctatgt cataagtttt accatagtaa gaactcattt ttttagctaa
     5521 ttgttccccc gacaaaatac aagtcacact ttccttacta tcatcaaaaa aattaacatc
     5581 agaaagcaag acatcataga cacgtcgcat ttcctctaga taattcctta ttgtcctgaa
     5641 aagtttgtct ttcattgatt caagagtgtc aaaaagtact tttgattcct ctttcaattt
     5701 tcgaaaatgt gtaggataca agtctaggtt ctctaatagc caattaaatg tttctacccg
     5761 actgtctttc tttaaaatta gcatcacaac tccaataaaa ttttcactga tatcacaatt
     5821 actggaatga caataaaaaa tggaatgccc agttttactg gtaaaaatac tagcagaggg
     5881 attatgatcg ttgtgaaaca gacatcttaa gtttttactt gacttaagta atttattcat
     5941 atcaatggtt aacaatcttt cataaatctc attaaaagac atgtcggagg aatcaaacag
     6001 gcagtcttta aaccactgtt tcatttcttc tttgtcgtca ttttttatca acttaacaaa
     6061 atcggtttca ttgactagtt catactctct ccctgaatca ctttgacaat ttttagaaca
     6121 tgtcatggta aaatcaatcg gaagctcagt aatatctaac atgttgtctt cattaattat
     6181 tatcactgca tctgacttat atcctccaaa aaacattcta ttcgaagcat ttacattgtc
     6241 atcacacccg gggatgttct ttttcaagta ttcgtaaaca gcaaccattt taccatagtc
     6301 agtaattgct tcttttaata gaaagacaat cttgtatttt tctagatttt cagagtaccg
     6361 aatacttttt tgtatcgcaa acgcagaatt atgaactaaa ttgatagata aaaccttttc
     6421 tatgcttaag tatctactat catctttact caaatctact accttcccct tctcatcttt
     6481 aacagtgtta tcgacatcca gaataattac tctggtttcc acaaaactct ccttgtcatt
     6541 gcctttattc tttgtcttgc ttggaataaa tgattgtgat gaatttagta tctctacaaa
     6601 ctcatctctt gtaacttctc tatagttatc taaacaccta tctcttacat cagttttttc
     6661 tttttttgta actggcttct ctaataattt ttctttatct acataaattg gaatattaat
     6721 cattgatgtt tccatttgtg tttcctcctt ataaaattct gttaaagact aaaacataca
     6781 gataataaaa acaaactctt tattactata tgctttagtc ttatttgttt actcaacctt
     6841 tataccgttc catgaaacta tcaatatcag attttttaaa tctaatgacc gtatcaattt
     6901 ttataacagg taacccgtat tgagaaacat attttctaat cgttcctact gatacaccta
     6961 gatattcact tgcctgctga tagtccatat actctagagt gactccagaa ataatgtctt
     7021 ctagcatagg ctttagcact gtaattatct ctttcacttg ttgttcaact tgtatagaaa
     7081 caatgctatc aatgatttgt agagacatat ttccccctcc tttcgtgatt gcttgaacaa
     7141 attttctaag aattatcttc gtattttttg ctcaaaataa aagtattttg ttattaaccg
     7201 attaatcaaa caaaaaatga ttaccagttc atcgacatac acataatacc tcgataaaaa
     7261 aggggtgtca atagttttgt aaaatttatt tacaacaccc cttttttgag gtatactaat
     7321 tttaggaggt gtggtcaata tgataaaacg tccaccaata aattatttag aacgaaaaaa
     7381 gatactcgga acaaaaataa aagctataag aaagagtaaa aaattaacac aaccagcatt
     7441 tggtcttatg attaacaacg gtcaattaat tgacaaaaag actatctacg aatgggagaa
     7501 gggaacctat ttaccaatac cagagcgatt atcccgaatt gctgatttgg gaaatatgag
     7561 tatagaggag ttagtttgtg gcaatgtaga agaatatatt ttgggaatta ttctttatag
     7621 agatagcatt gtactagatg gtataacatt tccagataaa aacctgttcc agcatcttcg
     7681 tcaacagttc ccaccagtac actctaattt agatacatgg ctggatagat attccaaatt
     7741 agaaccagag atgcaagaat ttattgctaa caagacttgt aataaagtta aaaatgaaaa
     7801 aattagtctt ttcaatatct taaaaataga agaattattc atcaatgcta ttgtagaaga
     7861 atttgacaat aacattcttt tcctcactag cagtatagaa gaactactag aaagaatggt
     7921 agatgaatgg ctacctattc aattgaaaga catgtcttat cccgaagaag ctgtgagaga
     7981 aattactgat aatataaaca agctagaaca aacgatttca tcaattggga aaaaatacac
     8041 taagaaaaaa atgaagggag gtgataccat ctagtggtaa gtactccttg taaactaaga
     8101 ggagatgatt tcatggcaaa aatatcaaag tacaaaaaga aaaacggtca agtggcgtgg
     8161 atgtataaag gacacattgc caccgatcca cgaactggtg aaaaaataaa tactacgaga
     8221 agaggtttta gtagcaaaca agaagctcaa aaagattatg acgagtacag acatcaaata
     8281 ttatacggtg ttaaaaagaa agcacctgat atgacatttg aagacctgta taatgaatgg
     8341 ataactcatc agcgtaccag tgttaaagct tcaacaatcg caatctcagt aagatacgca
     8401 aataatcaaa tacttccagc atttggtaag cttaaactat cgaatatctc tgtcccatat
     8461 tgtcaaaaag tggtagatga atggcacagt aaatatgaaa gttacgatta tatgagaaaa
     8521 cagactgcac aaattttacg ctacggggtt gcaatgcaat atattgacaa taatcctatg
     8581 gaaaaaacac ttcttccacg aaaaaaagag tatgagaaaa atcgtaaatt ttactcaaaa
     8641 gaggagctta ataatttact ggatgctttt aaagattttg gtaacatgaa acaatacgcc
     8701 ttcttcagat tattggcata cacaggtatg agaaagagtg aagtactcgc tttacagtgg
     8761 aaagacatag acacgtttaa taaagaacta catgtcaata aaactcttgc tgtagatgaa
     8821 tttggtaaag ttataattca atctccgaaa acaagagctt ctagacgagt tatctcttta
     8881 gatactgaga ctctttctat cctcaacaac tggaaattac agcaaaaaga agagtattta
     8941 aaacttggat acaatactag ctctaaagag caacatgttt ttacaacagt taaaaacact
     9001 ctatacattc caaacactgt taatgattgg ttaagatata ttttgaaaaa atataacttg
     9061 cctcgtatta cacctcatgg gtttagacat acacatgcta gtttattatt agaagcagga
     9121 gaatctgtca aggtagttca acaacgatta gggcatgaaa atagtaaagt gacattagac
     9181 atctatgcac atattacaaa taatgcacct aagaaaacag gtcaagactt tgctgatatg
     9241 atggctcatc agtaaaagag ctggtcaaaa tctggtcatt tctagtatac aggcaaaaca
     9301 aaaaagctag aacccttata tatcaagggt tctagctcct ataacaggtt tacccaacac
     9361 tgccttccaa tgactctttt gggaatcaga ggaaagaatc aggctttata gagatatttc
     9421 atctaacaaa ttactttaga ctgacagtat atatcagcag gtactgtcat cgggaacgta
     9481 gccaatatcg tatccaaaac agcaggtaga gaagctattt gaggattcaa gttattctat
     9541 ttcaaccatg aagttaagtt cattaaaaat aaagatagtt ggtatttcaa taatttttgt
     9601 atagagttaa tcactttatt gatgctaatt catcttgatt ggtattataa tcgtattcaa
     9661 gttgcgtggc gtgcttttgc tagaaaatat ataactgttc tttataaaga aatataagga
     9721 ttaaacattt tttatcatag agttttactt atctatagaa ttatgtctgt tcctaattca
     9781 gtttgatgaa ttaactgata aaaaaatatc tactatttaa agatacaggg ataagtaagt
     9841 aaaaaaataa catgtcatta gatagtagac agttccaact ctttatttac cttttttgta
     9901 tgctgcactg tcaatccaag ttcctaaatt gttgtatcca tcatcactta tataatcata
     9961 ttggccatca ataatttcgt caaaccacaa atattctcca tctggttttt gcccaattat
    10021 tttaattact gattgactag tagttgtcat ctggttaaaa tcttttattt tacctatatt
    10081 aacggccaaa atagcatttc ctcgcttata actctcctgg atttcatatt taacatattc
    10141 tcgatcaaga gtttctttac ctatcagcac cacagtcact gatgttccaa ttaattgatt
    10201 atctatccag ttttttatag caatatctcc attacgttta actttctcaa attcagcctt
    10261 atctacaaaa ccagcttcct ttgttcctcg caaagtccag ctgtttctaa ctaccattgc
    10321 tcttgttaca tctgcataat ggaaactaaa aaaaaccctt ctcaccatat taatcctcct
    10381 gaatactatt tattaaactg acaatttgtt caactttttt atctacttct ccttcagtat
    10441 ctaatttttc tagcgcttga aatttacttt ttaaggaatc atcaaaataa tcactgaaat
    10501 tctcttgaat cttatcaaac aaaaccttac ttgcaccttt ggtaaatcca atcggaacta
    10561 ctatattttc attttgaaca gataattcaa attcttcaat cattccatct gctgtaacta
    10621 tttcacctga ttcaagtttg tttccaaaaa caaaaatact aatcccagac tctgcaataa
    10681 tgttttctcg atatcttctc cactcatgag gcatatcaag gggaagtggt cttaaagaaa
    10741 gtacgtcttg tatgttattt tttcttaaat ttcttctctg ctttagaact cctgaaacaa
    10801 tactattacc aattccaagt ccgtaacctg atactattct ataaccggat tcagaaagcc
    10861 tatatgctaa tttctcgaca aattttaatc ctttcgattc agatgtaaat ggagtataat
    10921 caacagcact tcccgaaata aaaatagttt ttctcaaata cttttttcta atacttttca
    10981 aaatatcagt aatctcagaa taatcatcta cttcaacaac gtttatcgaa aatctggaca
    11041 aatcattgat ttgtagtctt ctttttgtca gttcataatt atatctctct tgatcaaaaa
    11101 aatcctcaga atcgacaaaa ttttccttta aaattatatg ggattttaga ttatcttttt
    11161 agaagttctt gaatgtcggt atagcccgtg tttccgggct tttccggcat ttttgatatt
    11221 gttagatact tacaaatgtt cttattacct ctcaccattt ccccctcaaa agtagtaaat
    11281 ggagtagtaa aaccggcctt gtgtcaggct gccagccgtt ccatttctgc ccttgcggaa
    11341 tcgaatgtgg cgtgtgcata atagttcagc gtcatggtga tattggaatg tcccataatg
    11401 tactgcaagg ctttggggtt cattcccgca ttggctaacc gggtgcagaa tgtatgacgc
    11461 atagcatggg gcgtcgtcgt tttcggcaac gcttcttcat ggctcttgtt gtacttcttc
    11521 acaagcctgc caaacatccc gccataatct accgccgtca tgggatagcc gttctgtttg
    11581 agaaacagga aattggcata cccgtcaata atgaacggct ttgcaccttt gcggtttttt
    11641 atcactcgct ggaacgcctg atacacttcc tcattcatgg ggatttttct gacgccgctt
    11701 tgggtcttgg gttcgtcaat atagtaaccg acgccggtat ttctcaaaag ctggtggctc
    11761 acgatgatta cccggttttc aaaatccaaa tctttgtccg tcagtccgca cagttccgaa
    11821 atgcgaagcc ctgtccccag cagaataacg attgcgtcat aatgcttttg gtaaacctta
    11881 tcaccttgaa taaaggacag aaaactttct tcctgtgctg gtgtaagagg cacctttggt
    11941 tccgtatcgt cgtccagaac gtcactaagc tggaagtcaa acgggttttt tcggatgcaa
    12001 tcgtcctgta tggcggtgta aaaagccgct ttcagggaac gcttgtcgtt attgatggtc
    12061 ttataagaaa ggcccttttc tttcatccga agcgcccatt cctttgcgtc ggacagcttc
    12121 acactgtcaa tcgggcagct ccccagccga tcttgttcca gaatacgcat cagccgttcc
    12181 cggcccagtt tggtgttgtg cttcacattg ccccggtgcc tgatctgctt ggcgtagagc
    12241 tggcagacgg tcattttctt gccgatggtg tctatcccgt cgtcaaggtc tttctggatt
    12301 tctttctcct tttcccggag tgaaatatcc tcccgtttac ctgccggggt cttatccgta
    12361 ggcactaact tccatgagta aacaaactgc ggttttccaa aggtatcaac atatttgtaa
    12421 gcatatcgtc cgtcttttct ctggctctct ccagaccgca aaacgcgatt tttgctgtct
    12481 cgtctttttt ctgacatctc gtcatgctcc tttccatgac ggaaagagcc gcatatatgc
    12541 ttataaatta ttataacata tctgcggccc tttttcacta ttagattgtg tccaaagtgt
    12601 caattatttt ctcaaactgc ttgcgtttga tctggatgcg gttgccattc ataatgaccc
    12661 agccagaggc cggattttcc tcggccagct tacgcaattt gttttctccg atacggaaat
    12721 atttagacgc ttcctcaatc gtcagcgtgt atttttccca aataggcaca tcatggttca
    12781 taatgcagcc ccctttcaca aattcaaagg tttgtgtgat ttaggggcat agacggacgg
    12841 ctgctggcac agctccacgg gactttcacc cccgcgtggt tctcacgcgg ccctacccat
    12901 tgcctgcgac gcttttaacg ctcggactgt ggctataagg gagtatcata gtcctgcctg
    12961 tacgtcgtcg cccgcaagcc gccacgctca cattgcggcg tggtgttcct cgctccgctt
    13021 ttcccgtagg gaaaagtatc atggcgcacc agccgtccgg ctcggcacag acagaatgtg
    13081 tccgtatgcc ctatgatgcg ccgccgttat cttgcgggca tatttatcaa ggtgcgatca
    13141 ggccaccggg aaaacaccga aacggggaaa gggtaagccg tttcatgctg ccggggccgg
    13201 ttcgtcagtt cgtgacgata gcgtggatga gcttgatttc cagacggcgc ttcatggcct
    13261 cgtccagaca cacatgcgga tagccgtttt cgtcatagag cgtccgggta cacagcttgt
    13321 tcatgtaacc gctgtaatac tgcaaaacgc gctctacggc tccggcatcc ccagcgcggg
    13381 cggcctcgat caccgaaagg ggcaaaagct caatgccctt gtagttcggg ttcttcatcc
    13441 gcgtctaacc tcccttcggc atcaacgcct ttagctgttt ccgcagctcc gataaggtat
    13501 ttgtcctgtg ccgctgaacg gcactccggg acattcccac aaggccgccg atctcaccat
    13561 ctgccatatc cagaacacag tgcaggatta aaatactctg ctccatgctg ggcagcgcgg
    13621 caaaggcttc ggcaacaagc tcattgttga tatgtaggtc atagccatgc gacgaaaacg
    13681 taaagctgtc gctggggtag tgatctactg tacagagctt gtccaaatct gcctgtgaca
    13741 aagagcttag tgacacttca cggtcacggc ggcgcttcat ttcagccaag tagcttttgg
    13801 cctcgttacg cagtaccgtc ttgcaaaaag catcgaacat gcaccgcgct tcatagtcgc
    13861 gggggatagc atccatcttc tcaccccctt tctgtggggg acttaggtgg ttctctccct
    13921 ttccgctaac aagactttga caaggccgcc agctacccgc taaatacctc ctttttgaaa
    13981 atttctcaaa aaattttttt ggggacagcc gggccgcaaa agacgacaaa aatcgcccgg
    14041 caggtcgcaa acctaccggg cgagaaaatg ctttgctatg ctgctgattt acatggtata
    14101 gtacatactc ataaccggaa tagccctgtg
//
LOCUS       DANJMI010000067        13731 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000067 DANJMI010000000
VERSION     DANJMI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.067"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(142..1344)
                     /locus_tag="PWU81_002518"
     CDS             complement(142..1344)
                     /locus_tag="PWU81_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1111185.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1347..1541)
                     /locus_tag="PWU81_002519"
     CDS             complement(1347..1541)
                     /locus_tag="PWU81_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL1111186.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1588..2436)
                     /locus_tag="PWU81_002520"
     CDS             complement(1588..2436)
                     /locus_tag="PWU81_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HDL1111187.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2641..2991)
                     /locus_tag="PWU81_002521"
     CDS             complement(2641..2991)
                     /locus_tag="PWU81_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111188.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3586..4032
                     /locus_tag="PWU81_002522"
     CDS             3586..4032
                     /locus_tag="PWU81_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL1111189.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4656..5648
                     /locus_tag="PWU81_002523"
     CDS             4656..5648
                     /locus_tag="PWU81_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1111190.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5664..6548
                     /locus_tag="PWU81_002524"
     CDS             5664..6548
                     /locus_tag="PWU81_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1111191.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6597..8204
                     /locus_tag="PWU81_002525"
     CDS             6597..8204
                     /locus_tag="PWU81_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1111192.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8205..9689
                     /locus_tag="PWU81_002526"
     CDS             8205..9689
                     /locus_tag="PWU81_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDL1111193.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9866..10858
                     /locus_tag="PWU81_002527"
     CDS             9866..10858
                     /locus_tag="PWU81_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1111194.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10910..11779
                     /locus_tag="PWU81_002528"
     CDS             10910..11779
                     /locus_tag="PWU81_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL1111195.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12199..13692)
                     /gene="guaA"
                     /locus_tag="PWU81_002529"
     CDS             complement(12199..13692)
                     /gene="guaA"
                     /locus_tag="PWU81_002529"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL1111196.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT         4214 a         2322 c         2832 g         4363 t
ORIGIN      
        1 tgaaaaacgt tgatttatcg gcattcccca tccatattat atttacgtct tattcccact
       61 caatatgagt agcctcgtaa agaagctatt atatcagtgc tttttcagcc tattcctcat
      121 tttggtaggg aaatggtagg gttagatttt tactgcttga tttccattaa agtttgcaac
      181 tttagatttt gttgcacagg taaatttaat gtttcctgtc atttgttctg caattccttc
      241 atcattacgg ctccatagat gagcgtagtg ctttaaagtg atttcagggc tagaatggcc
      301 taaccgttgc gacacgacca gaatgtctgc gttaaactca ttgattaggt agctaacatg
      361 agaatgacgt aatccttttc cttgtattag gggtacctta gctagtttgg cataacgctg
      421 aataattcgt tgaacagttg aacgatagag tgggaggtca gtgtagctaa gaataaattg
      481 ttctacacca tgattttttt gaataacttt ccactttttt agcacttcaa ttgtatcatc
      541 gtccagagaa atgatccgca agccgctttc agtttttgtg taaggtttcc gaataaaatc
      601 tttttgattt gtcatttcca aggtgtgatg aaccctcaac tttttacgtt tgaagtccac
      661 gtcattccaa ttaagtgcta atccttcgct tacacgaaca ccagtcatgt aatatagcca
      721 gatcattaaa aaacacatat gttcgtagaa atcatcttta taaatttttg ccaaaacttc
      781 ttcaaattct tcttttgtcc agtaaggaac gattgatttt ccttttggaa ttgcttttgt
      841 tcttttggaa atattctcat tcagaaattg cagggtgacc gcatagtcta atgatttacg
      901 aaacataccg tacataagtg aactgtatgc ttgtgagtat ccgctatcgt tcaataacca
      961 aattcgataa ttttcacaat ctcgcacatt gatttcacga agcttcttat caccgaaaaa
     1021 ttcagtaatc tgaatcaatc cacactcacg agaactccaa gtactttttt gtacgctagc
     1081 tttgtagtgt ggaacgtaag actgctccat aaattctttg taagttaaat cataattcgc
     1141 aaatccattt acaaccatgt attcattttt tatacgcatg acttctttgt gtgcttcacg
     1201 agctgatgag aatcgttttc ctagagaatc ttttgttcct ttcttctgga tcctctttcc
     1261 agtcacttga tctgttccta actcaactag ataagaaaag cgaccatttt tatcttgata
     1321 cacacaaggg tattttgttt tcatagttat tcctcctgtg aaagaggaac acctataatt
     1381 tcttcaatta cttgtcgggg tactacccca actcttttat tattataata tggatatccc
     1441 tgatttacca ttcttgtttt tgcttgtcgg attattgtgg tagcttgatg tgttttaaat
     1501 cctagagcct ctaaatcttt tcgacaaatt gtatttttca ttgtataaac tcactttcaa
     1561 agaaaacact atcattgggt aatagtgtta tttctttatt tttgtataat gaacggtact
     1621 ttcttgaaaa tccatcactt gttcaggact tcccaactca tttaaagcta aagcattgta
     1681 aaaaaatgga cgtctaacta tatattcgct tgtatatagt atatcttgat tcgtggaatc
     1741 atagggtttc tttaccatct ttttggtgac agttggtagt ttgagattcc gggacttgaa
     1801 aaaagctttt ttcttatgct ctttcaattc cagctcttta tcgaaatatt tgctgatata
     1861 gcgcccgcga ttttcgatac tgtctacatc gatcttgttg atcttaacga aaccctgacc
     1921 ccaaatcgta gttaaccggt taaatgagat aaaggggaaa tcaaacaaga taatatggta
     1981 gtggattgca ccgcgttgct gcttttccca cgtggcgaga tactttaaat ttgctttttt
     2041 agttttatag aggtcataat tcagtcgttt gataaatttt ttaaattcat tatttgcata
     2101 ctcaatgtct tgaatgtttt ctttgaaagt gagagttaaa aatttcgttt gattgtcaaa
     2161 attcgtgtct acaaggcggg caatctcaaa tcgcatactt tgataatgtt tctgtttgcg
     2221 ttttaaactg tcatattgtc cttgagcgga taattcatca tagttttttc ttttttcaag
     2281 agtaatatca ttttcataaa tccaagctaa tttttgtttt cgttccagat ttacttcgtt
     2341 ttcgatcgtt ttacgtttgg gagtaatgat tgcttcataa tcataaattt caatatacgt
     2401 gggagtttca atgattttct gattataagc ttgcaaaaat catcactcct tgttgcgtat
     2461 tcgattgtca aagaaccagt cattttctcc tgatgttgat ctataaatca agttaagggg
     2521 gaaggcctgc gtagcagccc ttctagccta tggggtgttg ggcggtctta acggacagaa
     2581 acggctctgt gggcgctgcc ccgccgtttc gtcctaacca cccaacactc catagtatag
     2641 ttagttttgt gtagtggtac cttgttttct gggaatcaaa gttgccgtat cagcggttgc
     2701 cgataaccca taactgacaa attgtccatt cgagcgtccc caaggtacaa tcaccaaatt
     2761 ggtaaatgta attatggcta aaccttgttg ggtaagttct tccggcgtga tcacaggatg
     2821 ttcagatttg actttgacac tgattttttc gtagcgaagt tttggtagga tacattcgta
     2881 gctccaacct agaatttcgc cctccttttg ccagcgagtg acattgacga cttggtattc
     2941 tttttcgagt gtttcttttg gtaaactgag atcggtaatt tttattggca tcacaattct
     3001 ccttttttat acgaaagttt atacgatttg attaaaaaat atagatggcc atctacgact
     3061 tgtattatat acgagaactt atacgtagtc aagagatttt ctaaaaaaat atatacgatt
     3121 ttttatacga gtgttataat gaaaaaaatg ataaggagga atttgaatat gtttggttta
     3181 gtacaaccta ataaagtgaa ggtgggccaa cgaatcaaag aaataaaaga cggaatgtct
     3241 ctctctttta cagaattagg gaatcgatta ggtttaaaaa agccgacgat cagttcctac
     3301 gttcaaggct atgcgttggc tcctaagagt gtgatcaacc aattatctag tatcagcgga
     3361 aaacctgtcg gctggtttta ttttgggcat gtggaagaat atatccgaga gtatttgttt
     3421 ctcattggtc agaaaaaaat agtggacgat tatcctgaaa tcattgagaa aataaagcaa
     3481 gaattttata cgggcgagtt caaaaatcct gcttgggaga atgagctagg ctatcctttg
     3541 gaggaattta tccaagaatg ttttgtagaa tattcgagtg aaatcatgaa ggactatgtc
     3601 gtacagatta cgaaagaggt acttgaagaa tccaaaacgc taaacaaact atcatcaaaa
     3661 gcaaaagaag aagctgtcac gattctttca tctgggatca ttgactatat cgagatgagt
     3721 ggtgagatca agtatggaga gaaagatcgc attgcgaaaa tggtgaaaga atcaataaaa
     3781 acctttgatg tgaaacaaga cctgacattt aatgattcct atttgattgg aaagttgatc
     3841 aatattcttg gtacagatga agatacggag ctgtttcttt cttctttatc aatgatattt
     3901 actgataagc atttttccac tcgatttggt ggggaagaat tagtcgaaat atttcaatcg
     3961 atgcgaccag cgttaatcaa actctatcgg gagaaaacac cagatgaatt ttatgattgg
     4021 tttgagaagt aaaataagaa aagtggctta gattacaagt tggtgaaaaa atttttttca
     4081 gcataacatc agtaatctat aaaggggttg ttgattatac tgtagaagtt aagtactcaa
     4141 gagatttttt gtacgtaaac aaagcaaaaa agggtgcagt ttcactagaa aatgtactct
     4201 tttttgtgat ttttcttagt tatagagttt ttttgaaact ttaaaaatga gaaaacatat
     4261 tgattttaaa gagtgtagac atagcttctt ttgctaaata gattaaactt actggtgagt
     4321 ggtcttgaac atacttttaa atcacatttt actgagtttt attggatttt ataattcatt
     4381 ggaaacctcg tacttaatct gtttttatct atcgactaga atctgtatat attccagcta
     4441 tcaatgaggc cgcagaaaag ggtgcttaaa aataatctgt tgtttaaaat attattatca
     4501 gaatacttag atacaaaatt ctggcacaaa gtaaaaaaaa tattgactta aaaaaatgga
     4561 tatggtaatc tataaacgaa aatagtggta acgtttccaa aatgcatctt cgttaaattt
     4621 ttattttttt aaacgaaaat ggaaacgttt ctaaaatgta ttgggaggag attgaattgg
     4681 aaaagacatt gggaccaggt ggtgagataa gggcggtaca acgggataaa aaaaatattt
     4741 cgaagtggga aaaattaaag aaacagttgc ggcgtgataa gtggatgtac ttgttgctct
     4801 taccaggtat catcatgacg tttatcttta agtatgtgcc aatgtacggt gctgtgattg
     4861 catttaaaga ctataatcct atgctaggaa ttttggaaag tccttgggta ggactagatc
     4921 actttaggga atttatcagt tcaccaaatt ttacgctttt gctgagcaac acattaaaaa
     4981 ttagtattta tggtttattg ttaggttttt ttccaccaat tattttagca ctgagcttga
     5041 atcaaattat caataaaaaa ataaaacaga aaatccagtt gattctatat gcgccgaatt
     5101 ttatctcagt agttattatt gtggggatgc tgtttttgtt cctatccgca aatggaccta
     5161 ttaatgggtt agtagaaaag gttacggggc aaccaattat gttcatgtct aatccagctt
     5221 atttcagaac gatttatatt ttatcgggaa tttggcaagg aatcggctgg tcatccattc
     5281 tttacaccgc aacgctggcg ggagtcagta ccgatttata tgaggcagca aatatcgatg
     5341 gggcaaatat tttacaaaag atttggcata tcgatttgcc tgcattgagg cctgtgatgg
     5401 tgatcagctt catcttggcc gccggcggta ttatgaatgt aggatatgaa aaagcctact
     5461 tgatgcaaac ttcaatgaac atccctgcct cagaaatcat ttccacttat gtatacaaag
     5521 tcggcttgca atcaggtgat tatgcctact ctgctgctgt tggtctgttc aatatgatta
     5581 ttaatgtcgt tttattactg attgtcaaca atataaccaa gcggctaaac gatggtcaag
     5641 gcttgtaatg aaaggagaaa attatgaata gtgctttatc aaaaatcgac cgtcaaatct
     5701 tatggttgaa tcgagtgatt gtaacttttt tagtcgtcat caccattgtt ccgctgattt
     5761 atgtactggt agcttctttt atggacccag ctactttgat caataaaggt ataagcttca
     5821 atccgaaaga ttggacaata cagggatatc aacgggtatt tgcagatgat tccattttac
     5881 gcggttttgt aaattcgttg ttttactctt ttagcttcag cttattgact gtatttatta
     5941 cgatgatcac tgcgtatcct ttgtctcgac ctaacttggc ggggaaaaag attattatga
     6001 ctttttttgt cattacaatg ttcgtgggtg gtggcttgat acctacctat ttattggtaa
     6061 agaacttggg catgttagat agtgtatggg cgattatcgt accaggtgct ataaatgtgt
     6121 ggaatattat cttagcccgt acctatttta gtgctttgcc aaaagaactt tctgaagctg
     6181 ctactatcga tggcgccaat gacttgcaaa tactgttcaa agtcatgctt ccattagcga
     6241 aaccaattat gttcgtattg tttttatatg cctttgttgg gcaatggaac tcatactttg
     6301 atgccatgat ttacatcaaa gatcccgact tacagccgtt gcaactcgta ttaagaaata
     6361 ttttgatcca aaaccaacct tcacaagaca tggtgggagc caatactgcc atggcagaaa
     6421 tgaaacaaat tgctgagttg atcaaatacg caacgatcgt catttctagt ctgccattaa
     6481 tcataatgta tcctttcttc caaaaatatt ttgataaagg tgttttagtt ggttctgtta
     6541 aagggtaagt attgttcact atttgtatga ataaaaatta taaaggagat tttaagatga
     6601 aagcaaaatg gatgctgcca atcgttttta cttccgtact aattttgggg gcttgtggag
     6661 gctcaaaaga aaagagtaaa tcaagccctg attatgaact agaaaatgtc tcttttccat
     6721 tgaaagaaaa agtatcttta aaaatgatgt ctcaaagtgc ccctttggct cccaatgatc
     6781 ctaatgacaa attgattttc caacgattag aagaagagac caatctccat attgattgga
     6841 caaactacag tagtgatttt gccgaaaaaa gaaatttgga tatcgctagt ggtgatttac
     6901 cagatgccat cttcaacgcc gctgcaggtg actatgattt attaaactgg gcggaaagtg
     6961 gcgttattgt accgattgaa gatttgatcg aagattatat gccaaatctg aagaaaattt
     7021 ttgatgaaaa ccccgaatac cgacaaatgt ctactgcacc tgatggtcac atttactcta
     7081 tgccttggat tgaagaatta ggcgaaggaa aagagtcgat tcatacagta aatgggatgg
     7141 cttggatcaa caaggagtgg ttggataatt tgggtcttga aatgccgcaa accactgatc
     7201 agttgatgac tgtgttggag gctttcaaga cgcaagatcc aaatggtaat ggtgaagcag
     7261 atgaaattcc ttttagcttt attaacaatg gaggaaacga agacttaaaa tttttaatgg
     7321 gggcattcgg tctaggtgat aatgatgacc acttagtcgt tgataacgat ggtcaggtac
     7381 aatttactgc taacaaagaa gggtataaag aagccatcaa atatttcaat gaaatgaata
     7441 ataaaggtct tattgataaa gaggctttcg aacaagattg gaatacgtat gttgccaaag
     7501 ggaaagaaca taagtatggc gtgtacttta cttgggataa agcaaatatc acgggtgcag
     7561 atgatagtta tgacgtcttg ccagtcttag cgggaccaga tggaacgaaa catgttactc
     7621 gtacgaatgg catgggtttc tcaagagatc gtttcgtaat tactagtgcg aataaaaact
     7681 tggaacttac agctaggtgg atagataaaa tgtatgaacc gttacagtct gtccaaaata
     7741 actggggaac ctacggtgat gaagcacagg cgaatatttt tgaattaaag gatggtatgt
     7801 taaaacattt gcctttagag gggacagcac cgggggaact aagacaaaaa accgaagctg
     7861 gaggaccatt agcagttctt gattcttatt acggcacagt cactactatg ccagatgatg
     7921 cgaagtggcg tttagatttg atgcatgaca cgtatttgcc atatattaca gaagacaata
     7981 tctatccacg tgtcttttta gaaaaagaag aaacagatcg attagcaaaa atcgaaacgg
     8041 atatgtggga ttacatctat cgcaaaagag cagagtggat caccggcggt aaaatcgatg
     8101 aagaatggga agaatacctt gctgaattgg aacgtgtagg tttggaagaa tggattcaaa
     8161 taaaacaaac tggctatgat aattttttga agggagcaca ataagtgtta gtagaagaaa
     8221 cggcgatttt ggataaagca aatcgattta ttgaaatgat gcgaggaaaa gttaaccctc
     8281 agtatcgaca aaactatcat ttttccgcac ccatcgggtg gataaatgac cccaatgggt
     8341 ttatatatta caaagggcat taccatctct tctatcaata ctatccttac gatagtcgat
     8401 gggggcccat gcattggggg catgcgcgca gtagtgattt ggtccattgg gaagatctac
     8461 cagtagcgtt agccccagat caaccttatg acagcggcgg gtgtttctca ggaagtgcaa
     8521 ttgaaagaga cggaaaacta tatttatttt atacaggctg tcaagaaatt gatggtatta
     8581 gccatcagca tcaatgtttg gcggtctctg aagatggtat tcattttagc aaatacagtg
     8641 gtaatcctgt ggtagcagaa aaacagctag gtgaaaatgg gctaatacaa gaatttcgtg
     8701 atcctaaagt aattgttcac aacgatggtt actatatggt agtagctact aaatctacac
     8761 agtctactgg tagaatagtg atgtttcaat cgtcagattt gtatacttgg cacttttact
     8821 ctgtgctgct agaagggact tcagagcaag gaataatgtg ggaatgtcct gatttattcc
     8881 atttagacgg acgagatgtc ttaatcatgt ctccgataca aatcaagaag aacggactgg
     8941 aatatcataa tatcagttct accatggctt gtattggtga ggtaaactgg gaaacaggtc
     9001 agatgatagt tgataatttc catgaaatgg attttggaat ggatttttat gcaccacaaa
     9061 caacaagaga tcctaatggt ggacgggtga tgattgcttg gatgcagatg tgggatcgta
     9121 cattgcctac acatgagtta ggccatcttt gggcaggctc tatgagtttg ccccgagagt
     9181 taagagtgaa aaaaaatcga ttggtacaaa aacccatctc aactgtatat agtcatttag
     9241 tttatcaaca aggcagtgaa aatattgcag tgactgacac atcagtcgta tttcgaaacg
     9301 cgctgtctga ttcttcatat ttacatctag tcgcagattt ggagaaagct gaagttttta
     9361 caattgaatt actgcggtct aagacggatt gtctcaaact agcctatgac actgctacac
     9421 aggtttttga attggatcgt cagaaactag gaagagagct tacaggggcg gaagaagacc
     9481 ctctgaatgt acgaaaagtt gaagtacctt tgattgataa tcagctcgtt cttgagattt
     9541 ttagagatac ctcatcaatt gaagtcttta ttaatgggtt agaagttatg acggctactt
     9601 tctatgagct ggaaaaagct cgagatatct tgtttcattc gatcggagaa agtcgtattc
     9661 aagcttttga aacagcgagt gtgaaataaa tgtttgattg cttaggcgat gaatcaatta
     9721 tttaataggt tttgaaggat aatgttttta gaaaagaaca gatagctaga tttcccatgg
     9781 atttgctatt agtattttga aaaaattagt aatttatgac aaaattcatt atactaaact
     9841 tgtgcagaaa tgtgagggat caactatgaa agcaacaatg aaagatgtcg caaaattagc
     9901 aggtgttgga gtaggaactg tttcccgggt attgaataac ggttctgtta aagcttctac
     9961 tagagaaaaa gttgaagcag caatgacaga gttaaactat caaccagatt tttatgcccg
    10021 ggggttaaag atgaagcata cgaacacggt tgctttgatt ctacccacgg tatggcatcc
    10081 attttttggc gaatttgcct accatgtaga aacagcatta gaaagacaaa actacaagct
    10141 ttatttgtgt aactccagtg gtaatagtga aaaagaacaa gaatatatcc agatggtcag
    10201 ccaaaataaa gtagacggca tcattggaat tacttattca gatatcgatg aatacatttc
    10261 gagtaatctg ccttttgtta gtattgaccg ttatttcact gaagaggtga tttatgtgac
    10321 cgctgacaat gcggccggtg gacgtttggc tgcaaatgaa ctttatcgcc gaggatgtca
    10381 gaatgtagca tatatcggtg ggtaccaaga aacaccgaat gaaacgaaga gtcgtcgaaa
    10441 atattttgaa atcgagtgcc gtcaactggg gatttcctat aaagcgttag atatgttaga
    10501 acctttagac catctctcag aacgtgtaac aaattttttg ttagagaatc ccgatatcga
    10561 tgggattttt acgatcaacg atccaatggc attagctgtc attgatatgc ttgaaaaaat
    10621 agggaaaaac gtaccagagg atgtccaagt tatcggattt gatggtcaaa agatgacaga
    10681 aggagatcct tatctagtct caacgattgc ccaacctaca aagcaaatgg cggaagtggc
    10741 agtggcaaaa ttaattgctg tcattaatgg agagacagta gaaaaacgta ccgtattgcc
    10801 gattgaattt ggcgaaggtg acacaacgaa acctttagtg agaaacgatt ttgcttagtg
    10861 caaatacgtt tctttttttg aaaatgagtg aatgaaaggt gtagataaaa tgttatatgg
    10921 tggaatagaa gccggtggaa caaagtttgt ctgtgggatt ggtaatgaac aattacaagt
    10981 gatagaacgg aaaagttttc ctaccacaac accagaagaa acgatggagc aagtgtttga
    11041 tttctttgat aagtacaaag gaaatatcgc atcaattggt gtaggagcat ttgggcctat
    11101 tgatattcaa atcgaatcaa gaacttacgg atatatcacc aacacaccga agttagcttg
    11161 gcaaaattat cattttgtag gagctttgga aaacgccttg tcggttccca tctattggac
    11221 taccgatgta aatgctgctt gttatggaga atatatggca gggtatggaa aaggtaaaaa
    11281 tagtgttctg tattacaccg ttggtacagg tgttggtggt ggtgctgtag tggatgggaa
    11341 aatagtcgca ggttttagtc atcctgaaat gggacatatg ctcttacaac gtcataatga
    11401 agatcaattt gaaggaatct gtccttttca tcatgattgt ttggaagggt tagcatcagg
    11461 acctgcgatt gaaaaacgct ttggaagaaa agcccagact atctcatttg atcatcctta
    11521 ttggacgatt gaggcagatt atcttgctca gtgtgtgtat aacacaacgt tactatatgc
    11581 accggaagta ataattttag gcgggggtgt aatgaaacag ccgcaactac tcgagttgat
    11641 tcgccaatcg tttaaagaaa aaatgggttc ctacgtggat acgcctactc tcgaagaata
    11701 tctactaact ccaaggcttg ctgacaatgc gggcttgatt ggctgcctag ctttagcaat
    11761 aaagaaactc aatcattaga atagtattta ttagaactat tcataacata ccggaagttt
    11821 aaaagggtat tctgaaactt aaatagtctc aattcattct tctttcttga gaatctgagt
    11881 agagactttt attctgtttc aaattaatat aattacgaaa tgatattgag tacaagaaag
    11941 ttctttttac cagatctttt aaatcgaaaa atctgatata tatatttata gtactaaaaa
    12001 tctgattaga taaaaagcta attcaatagg aaaataaagg tagtgaataa aagcaaagcc
    12061 cttgttagta gttaatctaa caagggcttt ggtagggaaa atggtaggaa accatcttat
    12121 tttttacgaa aatcaggaac tttagtaatt gaataatgtt gatttatagg tattcctcat
    12181 ccatattatg ttggctcttt attcccactc cactgttgca ggtggtttgc tggtaatatc
    12241 atacacgata cggttaacgt gtgcgacttc atttacgata cggacactga ttttttgcaa
    12301 gacttcccaa gggatacggg caaagtcagc ggtcatgccg tcaatagaag tgacagcacg
    12361 gatacctaca gtgtagtcat acgtacggcc atctcccata accccaacag agcgaatgcc
    12421 tggtaggacg gtgaagtatt gccagatgtc gcgatctaaa ccggcattgg cgatttcttc
    12481 gcgcaagata gcgtctgatt cacggacgat ttctaatttt tcttctgtca attcaccaag
    12541 gacacgaata cctaagcctg gtccagggaa tggttggcgc caaacgatgc tgtcaggcat
    12601 gcctaattgg gtacctaatt cacgtacttc gtctttgaac aatgtgttca atggctcgat
    12661 cagcttgaat tgcatgtctt caggtaatcc acctacattg tgatgtgatt tgatggtttg
    12721 ggcagtttca gttcctgatt cgatcacatc ggtataaagg gttccttgtg caaggaaaga
    12781 aacgccttct tcaccggcaa gtttggttgc ttcatcatcg aagacatata caaattcatt
    12841 tccaatgatt ttacgttttt cttctggatc agacacgcca gcaagttttg ataagaaacg
    12901 ttctttggca tcgactttta taatgttcaa gccaaatttt ccgccaagac tttccataac
    12961 ttgttctgct tcacctttac gcaacaatac gtgatcaacg aagatacatg ttaattgatc
    13021 gccgatcgct ttttgtaaaa gaacaccaac aacagaggaa tctactccgc ctgaaaggcc
    13081 taataaaact tttttgtcac cgacttgttc acgaattttt gcgatctcca tgtcgatgaa
    13141 gttatccatt gtccagtcac cagtacattg gcagacatct agtgtaaaat ggcgcaacaa
    13201 gtcatttcca tattcggaat ggcgaacttc tgcatggaat tggattccgt acattttgcg
    13261 ttctgcattt tggatcgatg cgatggggca atctttgctg gtagcaacga catcgaaatc
    13321 agtcggtgct tgggtaacta ggtcaccatg actcatccaa acggtttgtt tttcaggagt
    13381 gttttggaaa agggccgctt ctgcagaagt gatttccaat tctgcttttc cgtattcacg
    13441 gtttttggct ggtttaactt ttccgcctaa tttataagta atcaactgca taccgtaaca
    13501 gattcctaaa actgggatcc ccaaatcaaa aatcttttca tcaataccaa aggccttctc
    13561 atcataaaca ctgtttggtc cgccagaaag gatgattcct ttagggttca ttgcctttac
    13621 ttcttctgcg gtaatgcggt ggctcatcaa ctctgagaaa acaccgaact cacgaatacg
    13681 gcgagtgatc agttggttat actgactacc gtaatcaagt acgataattt t
//
LOCUS       DANJMI010000068        13665 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000068 DANJMI010000000
VERSION     DANJMI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.068"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            65..307
                     /gene="yaaA"
                     /locus_tag="PWU81_002530"
     CDS             65..307
                     /gene="yaaA"
                     /locus_tag="PWU81_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDL1111197.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            294..1418
                     /gene="recF"
                     /locus_tag="PWU81_002531"
     CDS             294..1418
                     /gene="recF"
                     /locus_tag="PWU81_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL1111198.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1415..3361
                     /gene="gyrB"
                     /locus_tag="PWU81_002532"
     CDS             1415..3361
                     /gene="gyrB"
                     /locus_tag="PWU81_002532"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL1111199.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3381..5852
                     /gene="gyrA"
                     /locus_tag="PWU81_002533"
     CDS             3381..5852
                     /gene="gyrA"
                     /locus_tag="PWU81_002533"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDL1111200.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6088..6387
                     /locus_tag="PWU81_002534"
     CDS             6088..6387
                     /locus_tag="PWU81_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL1111201.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6437..6949
                     /gene="ssb"
                     /locus_tag="PWU81_002535"
     CDS             6437..6949
                     /gene="ssb"
                     /locus_tag="PWU81_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL1111202.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6975..7211
                     /locus_tag="PWU81_002536"
     CDS             6975..7211
                     /locus_tag="PWU81_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL1111203.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7354..9327
                     /locus_tag="PWU81_002537"
     CDS             7354..9327
                     /locus_tag="PWU81_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDL1111204.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9333..9785
                     /gene="rplI"
                     /locus_tag="PWU81_002538"
     CDS             9333..9785
                     /gene="rplI"
                     /locus_tag="PWU81_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL1111205.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10059..11426
                     /gene="dnaB"
                     /locus_tag="PWU81_002539"
     CDS             10059..11426
                     /gene="dnaB"
                     /locus_tag="PWU81_002539"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL1111206.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12206..13498
                     /locus_tag="PWU81_002540"
     CDS             12206..13498
                     /locus_tag="PWU81_002540"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL1111207.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4557 a         2469 c         3010 g         3629 t
ORIGIN      
        1 gttttattgc gaaaaaaaag gtataataga gtatactttt aataatgaaa atgagggatg
       61 ggcattgaaa caacaagtca ttctaaatac ggaattcatg acgttgggcc aaatgttgaa
      121 agaagtcaca gttatcggta gtggcggaca agcaaaatgg tatttggcag aaaacactgt
      181 gttagtcgat ggcgaaccag aaaatcgtcg tggacgcaaa ctttatcccg gcatgatggt
      241 tgaagttcca gaagtaggaa ctttttttat ggtaaaaaaa ggaaacgacg ataatgaggc
      301 tgaataagct gtatttaaaa aattatcgaa attacgaaga gctgaacatc gaattttcca
      361 agaacctcgt catcttttta ggagaaaatg cgcaaggaaa aacgaatctt ttggaaagta
      421 tctacgtatt agctatgacg cgaagtcatc ggacaagtag tgagcaggaa ttgattggct
      481 ggactgacga tcaggcgatg attcaaggag agatcacaaa aggttcatcc actcttccat
      541 tagaaatctt attatctaaa aaaggccgaa aaaccaagat caaccatatc gaacagaaaa
      601 agttaagtac ttacgtggga caaatgaatg tcattttatt tgcaccagaa gatctttctt
      661 tagtaaaagg cagcccgcag atcaggcgga agtttttaga tatggaatta ggccagatca
      721 atccaatcta tctttatgat cttgtccaat accaagcaat tttgaaacaa agaaaccagt
      781 acttgaagca attgaatgaa aaaaaacaaa ctgatttgct ttatttggat attttgtccg
      841 aacaactagc agcgtttggc agcaaagtat tgaaagcaag agcacagttc gtcaagcgat
      901 tggagttttg ggctaattct ttgcatcagc aaatcaccca tcaaaaagag cagctggaaa
      961 tcgagtatct caccgcagtc gattctttag aaacacatac gcaagagcaa atccaagaac
     1021 aatttctcgc acttttgaac caaaataaaa agaaagacct ttttcgggga acaacgacag
     1081 ttggtcctca tcgtgacgat ctttcctttt ttattaatca gaaaaatgtg cagacttatg
     1141 gatcgcaagg acagcaaaga acaactgcac taagtgtcaa attagcagaa atcgatttga
     1201 ttaaagaaga gacaggggaa tatccaatcc ttttgttaga tgatgtgatg agcgagctgg
     1261 atgataatcg tcaactccat ctgctagaaa caatcgaagg aaaagttcaa acgttcttaa
     1321 caacaacgac acttgaccat gtaaaaaata aaatgactgt tgaacctgag attttttatg
     1381 ttcaacaagg gaaactaaaa ggagaagaaa ctgaatgaca gaagaaagaa gtttagtaga
     1441 acgcgctaaa gagtatgatg ccagtcagat tcaagtactt gaaggacttg aagccgttcg
     1501 taaacgacct ggcatgtata tcggttcgac tagttctgag ggtctgcatc accttgtttg
     1561 ggaaattgtc gataattcga tcgatgaagt attagcaggt tttgcaacga aaatccatgt
     1621 gattattgaa aaagacaaca gtatcaccgt tattgacgac ggtcggggga tacctgtaga
     1681 tatccaggca aaaactggac gtcctgcagt tgaaacggtc tttactgttt tacatgctgg
     1741 aggaaaattc ggcggtggtg ggtacaaagt ttctggtgga cttcacggag taggatcttc
     1801 tgttgtaaac gcgctttcta cacagcttga tgtcaaagta tacaaagaag gaaatgtgta
     1861 ctatcaagaa tatcgtcgtg gagctgtagt ggacgactta aagattatcg aacaaacgga
     1921 tcgccatgga acaacggttc actttacgcc agaccctgaa atttttacag agacaactga
     1981 gtttgatttt tcaaaattgg ctacccggat ccgtgaactt gctttcttga accgcgggat
     2041 gcgtatatcg attgaagaca aacgagaaga agaaccagtc atcaatgaat accattatga
     2101 cggcgggatc aaaagttatg tcgaatactt gaatgcaaac aaaaccgtga tttttccaga
     2161 acctgtatat ttggaaggtg aacagcaaga tatcgcagtt gaagtctcta tgcaatatac
     2221 agacggctac cattccaata tcatgagttt tgccaacaat atccatacgt atgaaggtgg
     2281 aacacatgaa tcaggattca agactgcttt aactcgtgtg ataaatgatt atgcccgaaa
     2341 acaaaagctg atgaaagaaa atgacgacaa cttgaccggt gaagacgtgc gagaaggatt
     2401 gacagctgtt atttctatca agcatcctga ccctcaattc gaaggacaaa caaaaacaaa
     2461 attgggcaat tccgaagtac ggacagtcac tgatcggtta tttagcgaac atttcatgaa
     2521 attcttgctg gaaaacccaa gtgtgggccg gcaaattgtt gaaaaaggat tactagcttc
     2581 cagagctcgt ctagcagcga aacgtgcgcg tgaagtaacg agacgtaaag gtgctttaga
     2641 gattagtaat ctgccaggaa aattagcgga ctgctctagc aacgatcctg aaaaatgcga
     2701 attgttcatc gtcgaaggag attcggccgg cggatcagct aagcaaggtc gtaatcgaga
     2761 gtttcaagcc atcttgccta ttcgtgggaa aatcttgaat gttgaaaaag caagcatgga
     2821 caagatcttg gcaaatgaag agatccggtc tttatttact gctatgggta cagggtttgg
     2881 agaagatttt gatgtatcga aagctcgcta ccacaaatta gttatcatga ctgatgccga
     2941 tgtcgacggc gcgcatatcc gtacattgct gctgactctt ttttatcgtt acatgcgtcc
     3001 tattgttgaa gctggatacg tctatattgc tcagccacct ttatacgggg taaaacaagg
     3061 gaagaatatt acgtatgtac aaccaggaaa aaatgcagaa gaagagttga aacaagtcgt
     3121 tgcttcactt cctgcctcac caaaaccaag cgttcagcgt tataaaggac ttggagaaat
     3181 ggatgatcat caactatggg aaacaactat ggacccaagc aatcggatga tggcccgcgt
     3241 cagtgtggac gatgctattg cagcagatca gatatttgaa atgttgatgg gggatcgagt
     3301 agaaccccgt cgtgcattca tcgaggaaaa tgcacactat gtgaagaact tagatattta
     3361 atcatgaagg aggaaaaaga atgagtgaag aaatcagaga aaacatccat gatgtcaatc
     3421 taaccagcga aatgaaagac tccttcatcg attatgcgat gagtgttatt gtcgcacgtg
     3481 cacttcctga tgtcagagat ggtctgaaac ccgttcatcg ccgtatttta tacggcatga
     3541 acgaattagg tgtaacaccg gataaagccc ataaaaaatc tgccagaatc gtcggagatg
     3601 tcatgggtaa ataccatcct catggagaca ttgcgatcta tgagtcaatg gtacggatgg
     3661 cacagccatt cagctaccgt tatatgctag tggatggtca tggaaacttt ggttctgttg
     3721 acggtgacgg tgccgccgct atgcgttata ccgaagcacg gatgagcaaa atcgctacag
     3781 aaatgctgcg tgatatcaat aagaacactg ttgatttcca aagcaactat gatgatacag
     3841 aaaaagaacc agttgtacta cctgctcgct tcccgaatct tttagtgaac ggaacaacgg
     3901 ggatcgccgt aggtatggcc acgaatatcc cacctcataa cttgtctgag gtagtagcgg
     3961 ctatcgattt gctgatggat aatcctgatg tgacgaccaa tgaattgatg gaagtattac
     4021 ctggaccaga ttttccaaca ggtggtttgg ttatggggaa atcaggaatc cgaagagcgt
     4081 atgaaacagg taagggttct attaccgtac gtgcaaaagt tgagattact gagatgccaa
     4141 atggtaaaga acggatcatc gtgacagaat tgccttatat ggtcaataaa gcaaaattga
     4201 tcgaacggat ttctgaactg cacagagaca agcgtatcga agggatcaca gatttgcggg
     4261 acgaatcttc ccgtgaaggt atgcggatcg tcattgacat ccgtcgtgac gccagcgcat
     4321 ccgttatctt gaataactta tataaattga catccttgca aaattcattt ggattcaata
     4381 tgttggcgat cgaaaaaggt gtgccgaaag ttttgagctt gaaacaaatc cttgaaaact
     4441 atatcgaaca tcaacgcgaa gtcatcacac gacggacggt ctttgaaaaa gaaaaagcag
     4501 aagcaagagc acacatctta gaaggtttgc gtattgcttt ggatcatatc gatgaaatca
     4561 ttgcgattat ccgcgggtct aaatcagatg acgaggcaaa agcaacaatg atcgaacggt
     4621 tcgatttatc cgatcgccag tctcaagcta ttttagatat gcgtttacgc cgattgaccg
     4681 gtttggaacg ggaaaaaatc gaaaatgaat accaagaact cttaaaactg attgaagatt
     4741 taactgacat tctggcaaga ccagaacgtg ttacagaaat catcaagaat gaattagcag
     4801 acttgaatca acgatttggt gataaacgac gcacagaatt actagtcggt gaggtcttaa
     4861 gtctggaaga tgaagattta atcgaagaag aagaaatcgt tattacattg acaaacaatg
     4921 gttacatcaa acgtttggca aataatgaat tcagagctca acgaaggggt ggacgtggtg
     4981 tccaaggaat gggtatccac aatgacgact tcgtcaaaaa tctggtttct tgttctaccc
     5041 atgatacatt gttattcttt acgaataacg gaaaagtgta tcgggcaaaa ggatacgaaa
     5101 ttccagaata cgggcgtaca gccaaaggaa tcccaatcat caatttacta ggtatcgatt
     5161 cgagtgaaag catccaggca atcattgcgg tgacgggaga agcagaagaa ggccactatc
     5221 tcttctttac gacgcgtcaa ggtacagtaa aacgtacttc tgtcactgct tttgctaaca
     5281 tccgaagtaa tggtctgatc gcaattggtt tgaaagaaga cgatgagctc gtcaatgtgc
     5341 tactgacaga tggtcaatct aatatcatta ttggaacaca taacggctac tctgtcactt
     5401 tcaaagaaga agcagtccgc gatatgggac gtacagcttc cggggtcaga gggatacgcc
     5461 tgcgtgagga agattatgtc gttggggcag ccttaatgaa ggaaaatcaa gaagtattag
     5521 tgatcacaga aaaaggttac ggaaaacgga caaaagtatc ggaatatcca gtgaaaggcc
     5581 gcggaggtaa agggatcaaa acggcgaaca tcactgaaaa aaatggtcca ctagctggat
     5641 tgaccactgt ttctggagat gaagacatca tgctgattac agataaaggt gtcattatcc
     5701 gtttcaacgt atcgactgtt tctcaaactg gacgttcaac attaggcgta cgtttgatga
     5761 agatggaagc tgatacgaaa gttgtcacga tggcagcagt cgaaccagaa gctgcagaac
     5821 aaacagttga agaacaagga tcagaagaat aaccatcaag actaagtgtg taagctagca
     5881 agaataaccg attaaaaaat ctggttattc ctgctggctt ttttgattag ttgaatttct
     5941 cttaaaatat tcgaaactgt tcattctgta ttgcttttga ccaaaataga gtgtataatt
     6001 agagcttgtg agtagtctaa ttaattagat aactctcctt gctcttagga acctaagagc
     6061 cgaaagtcca cagggaggtg aatatcaatg gaaaacacga aatatgaaat catgtacatt
     6121 attcgtccaa acattgatga agaagcaaaa actgctttaa tcgaacgttt cgatgcaatc
     6181 ttgaaagata acggagcaga agttatcgaa tcaaaagatt gggaaaaacg ccgtttcgct
     6241 tatgaaatga acggttaccg tgaaggtatc tatcacatcg ttaaagtatc ttctccatca
     6301 tcagcaaatg ctgtcaatga atttgaccgt cttgctaaaa tcaacgacga tatcattcgt
     6361 cacatgattg ttaaagaaga agcttaattg ttgtttcatg tgaaacaata gaaaacgatg
     6421 agaggagatg actattttga ttaataatgt tgtattagtt ggacgtctga cgaaagatcc
     6481 agatttgcgt tacacagcaa gtggtacggc agtcgcaaca tttactttgg ctgtgaatcg
     6541 taattttaca aaccaaagcg gcaatcgcga ggctgatttt atcaactgtg tgatttggcg
     6601 caaatcagca gaaactttag caaactatgc tcgtaaagga acacttttag gtgttactgg
     6661 acgtatccaa actcgttctt atgataatca gcaaggacaa cgtgtatacg tgacagaagt
     6721 agtagctgac aacttccaat tgctggaaag tcgttctgca tctgaaaatc gccaacaaag
     6781 cggcggattc caaagttctg gtcaatcagc tggcggattt ggcggaaaca ataactctaa
     6841 tcaaacatct caatcatcca acggtatgcc tgatttcgac cgtgatactt ctgacccatt
     6901 cggctcaagt tcaacgatcg acatttcaga cgatgattta ccattctaaa cgaatttgat
     6961 aggagggaaa tagaatggca caacaaagaa gaggcggacg taaacgtcgt aaagtagact
     7021 acattgctgc taaccatatt gaatatatcg attataaaga tatcgaatta ctaaaaagat
     7081 ttatttcaga acgcggtaaa atcttgccac gtcgtgtaac tggtacaggc gctaaaaacc
     7141 aacgtaaaat tactgttgca atcaaacgcg ctcgtatcat gggcttgcta cctttcgtta
     7201 gcgaagacta ataaatccta ggaattgaat gactgatcct tttgatcagc tattcaattc
     7261 ctttttttct gtaaaaataa cctctattcg tttagtcgct cgaatgacga aagttatggt
     7321 aaaatcaaat tagtcatata tgggaggaaa attatggaaa ataaaaacaa tcatttatcc
     7381 ccgaaacctc tcttccttat cctgtttata ttaatcgaag tattggtagt cttaatttta
     7441 ccatttcgtt ttgttagcgc ttcggtcatt ctattgatga atttatgggg tgttcgctat
     7501 tggttgaaaa tgaaacgttg gcttatttta aataaacgtg aaaaaatcca gcaggcttcg
     7561 actgtagctg aaaccaatct agcttatgtc tcagaagtca tgcctgtagg agtgattgct
     7621 tatcagccat caagccaaaa aatcgaatgg ctcaatccat tagcattgaa aatcaaagat
     7681 caaagtggat taacagagga agaattgcta gatacttttt ttactgcaaa agaaaaaaag
     7741 aaaaaccaag tctatctagc agataaaaca tttcagtttg atatggatac agtaaagggt
     7801 gttgtttatt ttaatgatat tacccaaaac cagttgttaa aactccatca gacgaacagt
     7861 cagccggtga ttggtatttt atctattgat aactatgcag atgccattga taaattagat
     7921 gacaaagaga tttcttattt gaactctttc gtcactacac tagtttctga ctggatgaat
     7981 gaataccatg tctttttcaa aagaatcaac gcagaacggt ttttcttcgt agcacagatg
     8041 atcgatctga aaaagatgat ggataaaaaa tttgatttgc tggatcgctt tcgagcagaa
     8101 gctgaaaaac agagctcgcc tatcaccctt agtatgggga ttgcttatgg ctcaagaacc
     8161 ctcgatgaaa tcggtggaga agctcagaat aatttagatg ttgctttagt ccgtggaggc
     8221 gaccaagttg tcgtgaaaga ggtaaaagaa ggagcaaaac ctgtttatta tggggggaaa
     8281 tctgctaaga atgtcaaacg cacgcgtgtt cgttctcgag cgatgagtac cgctttgcgt
     8341 cggatttttg atgaaacagg cgatgtgttt atcatggggc acaagtttcc ggatatggat
     8401 gctttaggct ctgcttttgg tgtcagttgt ctggcacgct tcaaccagaa aaaagcttac
     8461 atcgttatca atgagaacga gatcattcca gatgtagaac ggtgtcttga agaaatccat
     8521 aagcatcctg aattagaaat gcaactaatc tctccagaac gtgccataga gctgaaaaaa
     8581 gaagaggacc tcctcgtgat ggtggattac cataaacctt ctctatcgat ctctcagggc
     8641 ctatatgagc aatttgataa gattgtgatc attgatcacc atcgtcgtgg ggaagagttt
     8701 ccaagtaaac ctctgctgac ttatattgaa tcttcagctt cttcagcttc agagctagtt
     8761 tctgagctga tccaatacca aagcagcaaa acaaatcgct tagatcgttt tgaggccaca
     8821 ttattgcttg cagggatggt tgtagataca aatagtttca atgtccgtac aactgcacga
     8881 acttttgacg ttgccagcta tttaaggtct tgtggagcag acgcatcctt agtacaatat
     8941 ttattgagct cggatttagc gacatacttg gagatcagca atttgattgc tggaagcgac
     9001 tatgtgactt ctgatattgt tgtggcggca gggacagacg aaaaagagta taatagtgtt
     9061 atagcagcaa aaacagcaga tacattgctt tcgatgatca atatcaatgc agctttcgtc
     9121 attacaaagc ggacagacgg cttgattggg atcagtgcca gaagtaccgg cacgatcaac
     9181 gtccaaatca tcatggaatc cttaggtggc ggaggacact tcacaaacgc agctactcaa
     9241 ctggaaaata cgacagttga ggaaacaaaa cagaagctat ttgctgcaat caaagaaaat
     9301 atgaatcaaa tatatgataa ggagtaatcc aaatgaaagt aattttttta caagatgtaa
     9361 aaggaaaagg taaaaaaggg gacatcaaag aagttccagc aggttatgca caaaattatc
     9421 taatcaaaaa taacttggca aaagaagcaa cgaaaagcag tatcagtcag ttagcaggac
     9481 aaaagaaagc acaagaaaaa cacgaagctg aaatcttgga agaagctaaa aagttaaaag
     9541 aattcttgga aaaagaagat actgtagtcg aattgaaggc gaaagcaggg gaagacagcc
     9601 gtttgtttgg ttcgatccct tcaaaacaaa ttgctgaagg gttaaacaaa caatacaaaa
     9661 tcaaattaga taaacgaaaa atcgaattag aaaatccgat ccgttcacta ggctacacaa
     9721 acgtacctgt taagcttcat catgaggtga cggctacgat caaagttcat gtcgtacaag
     9781 aataacaaat cttgatagcg atgttcagct ggtaagtata accagaagga aaaaagacaa
     9841 accgaaattt atctgagaaa acagctgcag caatgaaagc aaaagctgca aatcgatttc
     9901 agattgattt cggtttcttt tttgggaaca gcaaaatagc ataaagaaaa cgagtataat
     9961 aaaagagaac tattttttta ttacactttc ggtacgaatg atgacttttc tctcattttc
    10021 ttgtaaaatg aagcaattga agacaaggag cgatacgtat gaatgaagtg tggcaggatc
    10081 gaatcccacc gcaaaatatt gaagctgaac aagcggtttt aggctctgtt tttcttgacg
    10141 cagaaacgat cattgatgcc atggagtaca tagagccaaa agacttttat cgtcgtggcc
    10201 atcaaattat ttttgagaca atgatcgagt taaatgatcg taatgaagcg attgacgtgg
    10261 ttactgtcaa agaccggctg gaacaagcca atttgttaga agatgccggt ggtttaagtt
    10321 atctctcaga cttggcatta gcagttccga cagcagcaaa tatcgtctac tatgcgaaaa
    10381 tcgttgaaga aaaatcattg ctgcgtacat tgatccagac agcaacagga atcgttacga
    10441 aaggattcga gcaaggagaa gaagttcaaa caattttaga tgatgccgaa cgcagtatct
    10501 tggaggtttc ggaaaaaaga aatcgaagcg gctttttatc tattgctgaa gtactgaact
    10561 ccacgattga aaatatcgat cgactagctc aaaacaatga agaaatcaca ggtctaccaa
    10621 ctggctatca aggtctggat aaaatgacag caggtttcca gcccgaagaa ctaatcattc
    10681 ttgctgcacg tccagcggta ggtaaaacag cgtttgcatt gaatattgct caaaatgtag
    10741 gaacaaaaac agatcgttct gttgcgatat ttagtttgga aatgggggca gaatctcttg
    10801 taaatcggat gctttgtgca gaaggttcga ttgaggccag ccatttacgt acaggtcagc
    10861 tttcggaaga agaatggtcg aatctgatcg ttgcgatggg gagtctttct cgagcaaata
    10921 tttttatcga tgatacacca gggatcaaaa tctctgaaat ccgtgcgaaa tgtcggaaac
    10981 tagcgcaaga aaaaggcaat ctaggcttga tattgatcga ttatttgcag ttgattgaag
    11041 gtaacggtaa agagaaccgt caacaagaag tatcagatat ctctcgccaa ctgaaaaaac
    11101 tggcgaaaga gctgaaagta cctgtcattg ctctttcaca gttatctcgt ggagtagaac
    11161 agcgacaaga caaacgacca gtgctaagtg atattcgtga atccggatca attgaacaag
    11221 atgcggacat cgtagccttc ttgtatcgag atgattatta tgaaagagaa ggcggagatg
    11281 acgatgatgg aaacgagcca aaaaatgaca acgtcatcga agtcatcatc gagaagaacc
    11341 gaagcggtgc acgaggaaca gttgaactac tgtttatcaa agaatacaat aagttttcgt
    11401 cgctttcgcc aagagaagaa ttttaaagaa gaggttgtga gccttgagca atcccgagag
    11461 ctctcgtaag aagatgcaag gagtaaccgc aggaacgact agagtactta atttaaacag
    11521 atctccaaag ggattgtgac aaaagtcttg tcacagtccc tttttccgaa taaacggtgt
    11581 tcaatcagct gacccttggt attagctcag caaacgtcaa aatatgagga actattttga
    11641 cgtttgtttc tctaatactc aggtcaaaac gctgatctta cgacctcttt tccttaaacg
    11701 gtgttctcct gccaactcct cggtcacaag tcacaatatc atcaaacatg agaagcagtt
    11761 ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct ctttcttaaa
    11821 cggtgttcaa aagcttgcat ctgcggtaat acgcccaatc aaacgaaaaa tatgagaagc
    11881 tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca acctctgatt
    11941 cacggtgttc caaaagtagc ttcttcgaaa aataagtcga aatttcacaa aaatttgaga
    12001 agcaattttc gtacattcct tcttatttct tgaagctgac cacttctgtt acaacctctt
    12061 ttttaataac cattctaaaa agataaagtt cgttttttaa gcgtttttat aaaataaaaa
    12121 taaaaatcat tcgattttat ttgaaatatg tatctattat tgatacaatg gttcaggatt
    12181 aaataatata gaacgaggtg ttcacatgtc atctgtagta gtagtaggta cgcaatgggg
    12241 cgatgaaggg aaaggaaaaa tcacagactt tctcagtgaa aatgctgaag tgattgcacg
    12301 gtaccaaggt ggcgacaatg cagggcacac aattaagttt aacggtgtga cttataaact
    12361 gcatttgatt ccttctggga ttttttataa agacaagatc agtgtgatcg gaaacggcgt
    12421 tgtagtcaat cctaaatcat tggttaaaga attagcttat ttacatgaaa aaggtgtaga
    12481 aacaagtaat ttgcgtattt ctgaccgcgc gcacgtaatc ttgccttatc atatcaaatt
    12541 ggaccaatta caagaagatg ctaagggcga aaataagatt ggtacaacga ttaaaggaat
    12601 cggacctgcc tatatggata aagcagctcg tgtaggtatc cgtattgcag acttattaga
    12661 taaagaaatt tttgaagaac gtcttcgttt gaatctagat gacaaaaacc gtcaatttgt
    12721 aaaaatgttt gattcagaac cacttgattt tgacgatatt tttgaagaat attatgaata
    12781 cggacaacaa atcaaacaat acgtcacaga tacttccgtt atcttgaatg atgctttgga
    12841 tgcaggcaaa cgcgtgttgt ttgaaggcgc acaaggtgta atgctggata tcgaccaggg
    12901 aacttatcct tttgttactt cttctaaccc agtagccggt ggggtaacaa tcggaagcgg
    12961 tgtagggcct tcaaaaatca ataaagttgt cggcgtctgc aaagcctata cgtcacgtgt
    13021 cggtgatgga ccatttccaa ctgaattatt tgatgaaaca ggagaacaaa tccgcgaagt
    13081 aggacgagaa tacggtacga caactggtcg tccgcgtcgt gtaggctggt ttgattctgt
    13141 agttatgcgc cattctaaac gagtttcagg tattaccaac ctatctttaa actcgatcga
    13201 tgtattaagt ggattggaaa cagtaaaaat ttgtacggct tatgaattag atggcgaact
    13261 aatctaccac tatccagcaa gcttgaaaga gttgaaccgc tgcaaaccaa tctatgaaga
    13321 attgccaggc tggtcagaag acattacagg atgcaaaaca cttgcagaac tgccagaaaa
    13381 cgcacgtaac tatgtacgtc gtatctcaga gcttgttggt gtacgtatct caacattttc
    13441 tgtcggacct gatagaacac aaacaaacat cttagaaagc gtctgggcgc aaatctaagg
    13501 ataaaaacga cgactgaatc aggacgttct taccagagcg agtcgaagtg tttatctata
    13561 cgataataat aacttgaaga ggtagtgaaa gagacgttta gccttattgc cgataggctg
    13621 tctcttgaac actgtttatt cagaataagg gactgtgaca aaaac
//
LOCUS       DANJMI010000069        13254 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000069 DANJMI010000000
VERSION     DANJMI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.069"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..369
                     /locus_tag="PWU81_002541"
     CDS             <1..369
                     /locus_tag="PWU81_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111208.1"
                     /translation="QLQEQEHRQSLNGKTEEPSLSEMIAYLKHKNILLVGGHVNFQNK
                     LTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYRVMSEISNKDKIFYL
                     DNHVNMNLTIKRIYDLLSLI"
     gene            420..791
                     /locus_tag="PWU81_002542"
     CDS             420..791
                     /locus_tag="PWU81_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111209.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            876..1076
                     /locus_tag="PWU81_002543"
     CDS             876..1076
                     /locus_tag="PWU81_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111210.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            1210..2436
                     /locus_tag="PWU81_002544"
     CDS             1210..2436
                     /locus_tag="PWU81_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111211.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            3286..4610
                     /locus_tag="PWU81_002545"
                     /pseudo
     CDS             3286..4610
                     /locus_tag="PWU81_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            4603..4950
                     /locus_tag="PWU81_002546"
     CDS             4603..4950
                     /locus_tag="PWU81_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111212.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            5296..6468
                     /locus_tag="PWU81_002547"
     CDS             5296..6468
                     /locus_tag="PWU81_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HDL1111213.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     gene            6597..7454
                     /locus_tag="PWU81_002548"
     CDS             6597..7454
                     /locus_tag="PWU81_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL1111214.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            7474..8034
                     /locus_tag="PWU81_002549"
     CDS             7474..8034
                     /locus_tag="PWU81_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HDL1111215.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            9007..9774
                     /locus_tag="PWU81_002550"
     CDS             9007..9774
                     /locus_tag="PWU81_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HDL1111216.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            9800..10345
                     /locus_tag="PWU81_002551"
     CDS             9800..10345
                     /locus_tag="PWU81_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111217.1"
                     /translation="MGERTQALITVKYGNSKTKVSVSIHYQWGYGRFMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            10346..10912
                     /locus_tag="PWU81_002552"
     CDS             10346..10912
                     /locus_tag="PWU81_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111218.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTIEGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            10927..11175
                     /locus_tag="PWU81_002553"
     CDS             10927..11175
                     /locus_tag="PWU81_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111219.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            11194..11433
                     /locus_tag="PWU81_002554"
     CDS             11194..11433
                     /locus_tag="PWU81_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111220.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            11450..12034
                     /locus_tag="PWU81_002555"
     CDS             11450..12034
                     /locus_tag="PWU81_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111221.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            12141..12422
                     /locus_tag="PWU81_002556"
     CDS             12141..12422
                     /locus_tag="PWU81_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL1111222.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            12412..12753
                     /locus_tag="PWU81_002557"
     CDS             12412..12753
                     /locus_tag="PWU81_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL1111223.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT         5040 a         1761 c         2584 g         3869 t
ORIGIN      
        1 caattgcagg agcaagaaca tagacaatca ttaaatggta aaactgaaga gccttcactg
       61 tctgaaatga tagcttatct aaaacataaa aacattcttt tagtgggagg acatgtaaat
      121 tttcaaaata aattaactga actattacca aatattaaaa tgtttgatgt aagcgaaaat
      181 aatggaaaga tacttaaaaa tttgaatacg tgtgatgcga tttttctata tacagattac
      241 ttgaatcatg gtctatatta tcgtgttatg agtgaaattt ctaataaaga taagatattt
      301 tatttggata atcatgtgaa tatgaactta acaattaaac gtatttacga tcttttatca
      361 cttatataaa gagagaagtg ataaatagga cacaaaattt ggaaagtagg taagtcacta
      421 tgagagaact aactttatct tctaaggaat tagaaaagaa aactgcttta ttaaacgaaa
      481 tatttagtgc tgattgtata atccaaagtt taaaagaaag ttcattttca aaactaacag
      541 atcaggaaaa aatttcttat atgctaagtt tagataatct aggaactaaa tatgatgaat
      601 cagcaagtag cgataagctt ttgattgaat tagtatcaag tcttagtagt tttggaaaaa
      661 tgaaaagatt taatgtgata ttagaaattt ttgcttggtt aatcccttca tttatttggt
      721 ttttttatat tttcatagtt gaatctgagt taatagccaa ggtctctctt ggactgttaa
      781 ttccgatata aataggttca gcaatttact ttaataactt gtatacacct attcaaataa
      841 aaaagcagtt attacatgaa ggattaagtg gtaatatgtt aaaaagaatt gcatgggata
      901 aagataataa aacagatatt gagcttaaag aatataaata caattatttc tcgagatgta
      961 tagatagaaa gttactgttt aaaaaagaag gattagaatt tagttttgat gaagatgaat
     1021 ttgaaaaatt agtgatggac tggcgtaaga atttactgga tacggtacaa ttctaaaagc
     1081 aattgaggtc gaaattccga tacttgaatc atgatctata ttatcgtgtt atgagtgaaa
     1141 tttctaataa agataagata tttcatttgg acagtcatgt gaatatgagc ttaacaatta
     1201 aacgtattta tgatctttta tatgtttata aacattaatg aggtgctaaa attgacaaca
     1261 ataactatga caaaaaaaat agaagtatat gttgatggat cacaagaaga aagggattcc
     1321 ttttttgatt ttttttggaa aaatagtaga gaatatgtaa aagcgttgaa tctggtatat
     1381 aaacagctaa tgtttgaaga gttgataaaa gatgcaatta aagaaatgga tcaaggttat
     1441 gctgagagag aagaaagtat tcaaaatcgg attgaaaagt tagcatatac tgatccaata
     1501 gatcaaaaaa aacttgataa agcaaaagct gaattatcta aattgagaaa tgaaaaaaat
     1561 aaagaagcga gagaaatatt aacgcaagcg atagggttga agagtcaaac gagaacacga
     1621 gatgtagcaa aaaaattaga ctttgacttt ggagattttg ttgattccgc aaatatgaaa
     1681 gctagtaaag attttggcaa tgattttatc ggaattgtga ccggacaaag aacgcctaga
     1741 atatataaaa atagaaacaa atatggatta ctacctttta gaggtcgaga tattaaaatc
     1801 cataaagatg ataagtttta tatttattta cctaaaggct ttaggtttaa agtgaattta
     1861 ggtagcaagc caagaaaagc aagagatgtt gcttcagttt tacaacacat tatggatggc
     1921 gattacaaat taaatcaaag taatcttatt tttgatggaa gaaaattatt ttttaatcta
     1981 acaatttctt ttgaaaaaag ctataatgaa atggaattca agaaaggtaa tgaattgatc
     2041 gtaaactttg cagatttgtg tagcggtgaa tgtttaatta atgataaatc tgtaatgtct
     2101 tttggtgata gccaatatat aagaaacttc aaaagtaaaa ttttagcaat gaaatcaaaa
     2161 gaacaaagcc gaggagtctt ttctaagggc ggtcatggtc gaaaaagaaa attagatcaa
     2221 gatcgatggg aagcaataaa gaaaagagaa agtaattttg ttaaaactta taataatcaa
     2281 tgttctgcaa aaatcgttaa aactgctatt cgcaataaag ctcaaattat tcgtatcatt
     2341 gaaccagata aggcttatgg cgaatgggct tactatcaat tgaaagaaca actagtaaac
     2401 aaaggaaaaa gatatgggat agaagtcaga agttagtaaa ttaaacaatc gttgatttgg
     2461 caacgaaata gtgagggaaa atccactcac tgaagggtaa actccgtcaa gttgtgtgac
     2521 cagacggaag gacgaatgtt gttgaggttt tagaaccata taaaacagca tgtttctcaa
     2581 atttttagga taagggaaat tgctagcagt ttgttttaga accatataaa acagcatgtt
     2641 tctcaaatga gtactttaaa ttaattgatg gcgttcctgc tttagaacca tataaaacaa
     2701 catggattta aaacaagaag taagtcagtt ctccttttac tttagtaagg aaaaataaag
     2761 tattctcaat aaaacttaaa ataagcaaac ctttgtttta atagctaatc gaagtaaaac
     2821 ccaaagccat atgaacccct gaatcagcga aagtttatga tgatttggat tactaatagt
     2881 ttaggaatta gatggtaaat ctaaattaaa caatcgttga tttggcaacg aaatagtgag
     2941 ggaaaatcca ctcactgaag ggtaaactcc gtcaagttat gtgaccagac ggaaggacga
     3001 atgttgttga ggttttagaa ccatataaaa cagcatagtt ctcaaattgt ttaaaatgat
     3061 tgtcaagtta aaaaaatctg ggatcatata aaatagtata gttctcaaat atgattagaa
     3121 accctattaa agagcaatat taagccaact tacttaaatc gtttaattaa gaacaataat
     3181 ctcttttagt cagactttta gtctgacttt tttgtatttc caaaatttat tctttacaaa
     3241 aacgaacata cgtttgtata attgcttgtg aatgggggaa taaaaatgtt tgattacagt
     3301 agagaaccgg tgaatgacta ttttttaatc gacatgaagt ctttctatgc tagcgtggaa
     3361 tgtattgaac gaaatttaga cccaatgact acaccgttaa ttgtcatgag tagatcggac
     3421 aacactggtt caggtctgac tctagcttct tcacctactg caaagaaaaa atatcatatt
     3481 actaatgtta gtagagctac tgatttacct tatccattac caaaagaact tcatattgtt
     3541 ccaccacgaa tgaacttata tataaaaaaa atatggaagt taatcagatt tttagaaagt
     3601 atgtcgcaga tgaagattta ttgatttatt cgattgatga atcaatttta aaagtgacaa
     3661 aatccttaaa tttatttact agttcaggaa caagaagcga gagaagaaga gaattagcta
     3721 aaataatcaa agatgagatt cagaaagaaa caggtctaat agcaacagtc ggaataggag
     3781 ataacccatt attagctaaa cttgcactgg acaatgaagc gaagagtagt aaagaattta
     3841 tagctgagtg gacttatgag actgtacctg aaaaaatatg gaaaataaac gagatgactg
     3901 acttttgggg cattggatca cgcatgaaaa aaagattgaa tcatatggga attatgagta
     3961 tagaagattt agcaaattgg aatccctaca caattaaaag tcagttaggt gtaatcggac
     4021 tccagttata tttccatacc aatggaattg atcgtacgga tattgctatt ccaccagaac
     4081 caaccaaaga aaagtcttat gggaattcac aagttctacc aaaagattat acgaggagag
     4141 aagaagtaga actagtagtc aaggagatgg cagagcaagt agccattcga attagacaac
     4201 acaattgcaa aactggttgt gtacgcttag caattggtac gtcaattttg gaagaaaaat
     4261 caggtttctc gcaccagatg aaaataccaa ttacagataa tacgaaagaa ttacaaaatt
     4321 attgtctttt tttattcaat aagttttatg aggggcagga agtacgacat ataggagtaa
     4381 cttatagcag attagtatac acagattccc tacaattaga tttatttagt gatccaagaa
     4441 aaaagattgc tgaagaaaat ttggatagga ttattgataa gattagacaa aaatatggat
     4501 ttacagctat tatacatgct tcatctatgt tagaaggtgc aagaagtatt acgagaagca
     4561 ctttaatagg aggacacgca ggaggattag gaggtttgca taatgattga atataacgat
     4621 agaggacgtt taaaatggtc gggattttat ttgtctgatc atactgaaag aatcaatgca
     4681 gacaatgtac aacgtgctaa tcataatttg gcaaaagaac aaatgacgac tgaagagatt
     4741 gcgaaaatac tgagaactgc tgtcataaaa aatagacccg tctgcattca aaaagaggaa
     4801 cgaaacgctg aaggttacta tccagatgat gtagtaggaa aaattattgg atatgacgag
     4861 cttggtgtat ttgtggaaga agaaaaagtt cactatgatg aaattaggaa tgtttctttc
     4921 tcggaattat taaaatggag tcagatatga acattattaa aaaatacgaa ttcggttata
     4981 ttacgtatga acagctaata gaggaattat ggggatatgg tcaacaatta attaatgaag
     5041 tggggattga ttgcttttgt ttctatattg aatccggagc aggataccat aagaacagat
     5101 actatatatt tccatatcca ccaaaatagt ctcttagata tttttaaatt ttctccaaat
     5161 actttcaatt attgtgaaat atttcccttt aattgataaa gtccgtataa ttgtgtaaaa
     5221 gtaaaaaggc catataacag tccttttacg gtacaatgtt tttaacgaca aaaacatacc
     5281 caggaggact tttacatgac ccaagtacat tttacactga aaagcgaaga gattcaaagc
     5341 attattgaat attctgtaaa ggatgacgtt tctaaaaata ttttaacaac ggtatttaat
     5401 caactaatgg aaaatcaacg aacagaatat attcaagcaa aagaatatga acgaacagaa
     5461 aaccgacaaa gtcaacgaaa tggctattat gagcgcagct ttacgacacg tgtaggcacg
     5521 ctagaattaa aagtacccag aacacgtgat ggccattttt cacccacagt gtttgaacgt
     5581 tatcaacgaa acgaaaaagc cctcatggct tcaatgttgg aaatgtatgt atcaggcgtt
     5641 tcaactcgta aagtatcaaa aattgtggaa gaactttgtg gtaaatccgt ctctaagtcc
     5701 ttcgtttcta gcttaacaga acagctagaa cctatggtta acgagtggca gaatcgttta
     5761 ttatcagaaa aaaattatcc ttacttaatg accgatgtac tctatataaa agtacgagaa
     5821 gaaaatcgag tactctcaaa aagctgtcat atagcgattg gaataaccaa agatggcgac
     5881 cgtgaaatta tcggcttcat gattcaaagt ggcgaaagcg aagagacctg gacaacattt
     5941 tttgaatacc taaaagaacg cggtttacaa ggtacggaac tcgttatttc tgatgcgcac
     6001 aaaggattag tctctgccat tagaaaatcc ttcaccaacg taagttggca aagatgccaa
     6061 gttcacttcc taagaaatat ctttaccacc attcctaaaa aaaattcaaa atctttcaga
     6121 gaagctgtta aaggaatttt taagttcaca gatattaact tagcgcgtga ggctaaaaat
     6181 cgattgattc atgattatat cgatcaacca aaatattcaa aagcttgcgc atcattggat
     6241 gatggattcg aagacgcctt tcaatatacc gtacaaggaa attcccacaa tcgactaaag
     6301 agtaccaatc taattgaacg actgaatcaa gaagtacgca gaagagaaaa gattattcgc
     6361 atcttcccca atcaaacatc agccaatcgc ttaattggag ccgttcttat ggacctacat
     6421 gatgaatgga tttattcttc aagaaaatac atcaattttg ataagtagaa atggtaaaaa
     6481 cattgtatag cattttacac aggagtctgg acttgactct ttaattaatc aaatttttta
     6541 taaaagtaat atataaaata aattgcttga agtattatat ttagatatca taatttatgg
     6601 aagaaaaatt aattaaaaag gatgagatta taatggggtt actggttccg agagctaata
     6661 cttatactat acctttaacg aaacgccaat taattgctaa aagatatcaa aggattacta
     6721 gagccatcaa tagagagttt tggaattctg aaagtgatac tgctcacagt ttatacgtag
     6781 gttcttatgg gagaggaacg gcaattagta ctagtgatat agatataata gttgaattac
     6841 cgatggcaga atttgacaga ttcaaaaatt atttatcaaa tggcccgtct aagttattac
     6901 aagttataaa aaacgcattt caggaaatac taccaaatag tgatattaga gctgatggac
     6961 aagttgttaa aataaatttc catgatggaa tcaaatttga aatagttcca gcttttaatg
     7021 aaaaagatta ttggggtgag tcaaaagggt ttatttatcc agattcaaac atgggaggga
     7081 actggaaagc tacaaatccc aaaaaagagc aagaagccat gaaattaaag aatacaaaaa
     7141 gtaataattt attatatgct acgtgtaaac attttcgaca tgtacgagat acagaattta
     7201 cgagttatca tctttcagga atagtaattg acagtttcgt atatgaggct atgggaaatt
     7261 ggaaattcgt tgaaaataac agtgggggtc aaaacattag ttctgtatct tatgagacag
     7321 ctttattaga atactataac tcacataaag ttatgggcgg tttaaattta tactctcctg
     7381 gaagcaatca atttgttaat tctgatagta gtatcatatg tttagaaaaa gtattaaaaa
     7441 aaatagcttt ataaagtttt atggaggaac aacatgggag acgaaaaaaa gcgcttggaa
     7501 gttcaattac gtgaagcata tggacgagta acgtatactt acaccactca tttaaagcaa
     7561 gccgataata ttatttctga caataaaatt ttaaggtgtc ttcaaattgg tttgtcagcc
     7621 cttactactg gaggggtact tagtaccatt attactgata atagtgagtt aacctttgtt
     7681 gctggattat gttcaacaat ttctcttgga ttaaacttat actttaagaa ttttgatctt
     7741 gctgaagaag ctaaaaatca tcaaattgtt gccgataaat tatggttgat cagagaagaa
     7801 tacgtaagct tactaacaga tttaaatcgt ttaagtgttg aagatattgt gaaaaaaaga
     7861 gatcaattaa tgaacgaaac tcatgaagca tataaaaatg cccctaaaac aaatagtaaa
     7921 agctatgaat cagctcagaa agcattaaaa tctgaagaag agcaattttt tacagaagag
     7981 gaattggata agatgttacc agagcatctt agaaaaaaga gttctaaata ttgaactctt
     8041 tttttatgct taaaattctc ttgtaatttt tctattcatc tatatttaag aagaaatatg
     8101 atgatagaaa catctcctaa agccaccgca agccgttcta agcatagctt agcccgacat
     8161 tgctagatat gctcatctca ccatgaacgt ttcctaaagc caccgcaagc cgtcctagcc
     8221 attgcctagt ccgacatcgc tagattagtg catctcgcca taaaagtttt ctaaagccac
     8281 cgcaagccgt cctagccatt gcctagtccg acatcgctag attagtgcat ctcgccataa
     8341 aagttttcta aagccaccgc aagccgttct aagcatagct tagcccgaca ttgctagata
     8401 tgctcatctc accatgaacg tttcctaaaa ccaccgcaaa ccgtcctagc catcgcctag
     8461 tgcctagtcc gattttgctt gatcggttcc atttctgttt atttagtggg tcaacaccgt
     8521 aatcttaaat agccatcgcc tatttaagat tactaggtct gctttgaagg taaaaagtct
     8581 ttcattcttc aagactttta ggctttcaag ctacgcctga aagcattgaa attcatcgct
     8641 ctttatttgg ttttgccatt tataattcct tacgcccatt atctttttcg cctgtcttgc
     8701 cttgtcagaa gtgtttagag cttatctcat ctttggatta atacaagtgg cgtaaatttt
     8761 tttctaaacg caagcgttac gaaaataatt tacttaaaca cttgtattaa tccggcagat
     8821 aagaacgcat tctaatcact cccgacaagg caagacaggc gaaaaagata aataaatgaa
     8881 cgaaaggaag aaacataaaa tgcaaaacca aatagcgatg aagaaaaaac cacctccctc
     8941 taaaacaatc caaacaaaaa agaattttga aaaaccaaaa atcaaaaaca gaaagaagcg
     9001 ttcaacatga gagatagaat tttagaagtt aatcaagaaa tggtaaacaa gttaacaaat
     9061 atcagtggga actatgaaaa gtacgatggt actttacttg ataattatat ttttcacgat
     9121 acacaaaata tcagaattgg cagaagtaaa ccgagagcgt tcattctcat caaagagaaa
     9181 tatttgaatg aatggaatag cacctatgaa atggttatga ctgataactt tgaaaaggta
     9241 ttggatttca gaaatgcttt gttgggttct gatatgggaa atagcagtct tgaatctatt
     9301 gaagttgaaa acaagaccga gaatgaaggt gaaggattga ttaagaatgt cctgaaaatc
     9361 gaacaaaaat atatcagtga agatagaagt ttttatagag tcacaagctc ggttggtcta
     9421 gcaaactttg ctactaactt aattggaaac aatgctagtg aatctttgct agtgatgtgt
     9481 ctgaatacaa agaatgaggt cattgcttat agcgaagttt tcaaaggtgg aattaatcaa
     9541 tcagtagcga taccaagaga gatttttcaa ctagctttgc taaacaattc agcaaaaatc
     9601 gcagtatcac ataatcatcc gagcggatat gaatttccaa gtgaaaatga caaaagtttt
     9661 actacgaatt tgaaaaacgc aggaaaaaca attggtattg aattgttaga ccatatcatc
     9721 gtaacaagta aaaacacaag ctattactca tttcaagaaa atggttcttt ataaaaatta
     9781 gagaaaagag gaaaacaaga tgggcgaaag aacacaagcg ttaattacag tcaaatatgg
     9841 taacagcaaa acgaaagtaa gcgtgtcaat tcattatcag tggggatatg gtcgattcat
     9901 gttgatggac gttttgaatc aaggaatata tttaatcaat aaagcatatg acttacgaga
     9961 tgaatcaaga gaagaattag caaaaggaat ctttgataga agtacaggag ttatcaattt
    10021 tgttacctat gaatctggag tgagtgctta tgatgaaaca gggtttggtg ggcgctatga
    10081 accttatcaa gaaacgattg aagatgtgat gaagttttca gataacaatg atggctatgc
    10141 tcatttagag atggtgttcg atgaaaatgg atttagtcta gatgataaaa ccagattgcg
    10201 attgtttgat tttgagggtc aacagatagg actaaacaag ttttgtgaaa gcggaaaaga
    10261 gtttgctacg aaagatttta ttaagggata cagattattg ctagaaaatt atggaataaa
    10321 cctagaagaa gagagcgtga aataagtgtt tgaaaaaaga aaagaccagt tccctaattt
    10381 agaagcgaaa agtcaaaaag tgattgatgg tgaaatgatt gaattattcc aaaaaagtta
    10441 tggaaccata gaaactaccg caagttgcaa atactacttt caaggaaaga gaaacacgtt
    10501 taaaggcttt gccgattctg atgaagaatt ggcaaagaaa atcattgatt ggcttgtcag
    10561 cagtgaacac gtagcaagag aagataccgt tagaacaacg attgagggtg aagtcctaac
    10621 aggtactaga tgtgtttgtt gtaatcatga atttgtagaa gatgaaggtt gctaccgaat
    10681 caatgaatat ggtgatttta tttgccagag ttgccacgag atagaatttc caaccgaaag
    10741 aagttggttt gaatacatgc tacaagatta tgagatggaa acagaaaatg gtgaagtagt
    10801 tttttgtacc aaagaggaat tgagtaagag aagcgatgat gaaattgaag attggtgtgt
    10861 gtggttagta gggacagatg attgcgttgc atattacaca acagcagaat aggagtgtga
    10921 atcagcatgg aaggaatgga ttttatagat catgaagatt tgattgattt tggttataca
    10981 tggaaaggaa tggtaggtat tagcagaagt cttgctaatg ccttttacga aagaaattat
    11041 gcagtctatg tcctttatga tgacaataca gaaagccttg ttgatgaaga gtataaattg
    11101 gatttagaga atgttttata tgggatagaa aaagaagatt tagcaaaata tattttttca
    11161 tggttgggtc aataaacgaa agagggtagc aacatgacaa aattcaaaag taaaggtatg
    11221 caatggtgtt cttattatta tgttgggaat gatgaagagc ttttgagaaa agccaaacaa
    11281 aaaggttatc cagataagat gttaaaacag attggctatt acatgccaga agagagaata
    11341 gaaaaagaat cttgtaaggt tctttttgat agtgaacagc tattagtttt gttaactcaa
    11401 aataaattga tttgtcaatt tgaaaataat taatatgggg tgaagaacca tgaatgagaa
    11461 taagatggta ttaagtgaat acgcaagatt gagaaccatt aattggttga atggtatgca
    11521 aagtagtggc gcttttgatg gatttgatga ggagaatatg gactttgaaa gtttatcgaa
    11581 tgaggatatt agatattatt ttatcgaatg gtattttaca gaaccagatg aagaggtgga
    11641 agatgaacca gaattaattt tagaacgtca aagaatttta ttcagtgaac aagatagtaa
    11701 gaggttgtat ggtttatatg acttacaact gaaaggatta aaagcgcagt atgttgctga
    11761 tagttacgat gatatggtag aacagggggc agatagggtt gtcacttatt cagaagatga
    11821 tgaagaggaa tgggaaaact attcagatga tgagatatgc tttaatgatg ggtatatgcc
    11881 tattgaaatc accgaagaag aatatgataa agtgtcggat agcgatgaga ttggtatgtg
    11941 tacggttgta agtggattgg atatagatgt ccaaagagta gaggttgcat accagaagat
    12001 gaaagaacgt ataaaaagta aagtagaatc ttgatttgat tggattgttt gcaaagtagc
    12061 taattaagag tataatataa tcataaatgt agttaatgga tataataatg agtatattat
    12121 actcaaaaga ggtgaacgaa atgaaggtaa aaaatccaaa agaaaaaagc agaattcaag
    12181 tcggaataga caaggattta aaagaaaacg cagaaatgat attggaagag ctagggttaa
    12241 atccaacaac agcaatcaca atcctttata agcaagtggt cgctagaggt gagtttccag
    12301 tagagataaa gctaagcgaa gaggaaaagc aaggaatcag attgcaacag ctaactatgg
    12361 atatgccagt tgatgttcta gatacagatg aaaagttaga agagtggttc aatgaagcct
    12421 aatgagatag taacggtcta tgttgcattt acagataaaa atggtggtaa aagaagacct
    12481 attttggtgg ttagtgataa agaagataga gtagaatttt ttggaatcac aagtcaatat
    12541 gagaataaat cagaccaaat aaagagagtg tattttccta ttgcagaatg ggaaaaagta
    12601 ggtctaaaga agcaatcatg gattgacgta gggtctttaa aggctattcc caaaagccga
    12661 gagaacatat catttaaaaa ggttggtatg ctatcagcta ttgatacgaa aaagctaaat
    12721 aggtttatcg agagattaca aagaaaagtc taacaaggct tttctttttt tgtttatatg
    12781 tatattaaga tatacgttta tataaacgta tttatgtata ttaaaataca cgtaaatata
    12841 tacgtgatta taaaaattaa gaaaatatta agtattgtta aatagtgata attagtaata
    12901 tacgaattat aagatacatt ttatatttaa tgtatgtaaa taaataaaat gactgacgga
    12961 tattgaatag taataaataa caataattca gacggataca gtgagtgaag tagcttgcta
    13021 cgaacgaaca ggcagaatga aacggtagtt tcattagagc aagtcgcttg cgacgtagcg
    13081 ttaccactga tgtaactggc aagttacata gagagagttt gcttgcaaac gaacgttacc
    13141 cacttgaata gaaaacaaaa ataacatatt agtgaatttt gaataggagc cacaactcaa
    13201 ccaaccgctt gcggtgggtg agtcccacgg aggaaaagac gaacgaacgg tcgc
//
LOCUS       DANJMI010000070        13226 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000070 DANJMI010000000
VERSION     DANJMI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.070"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            158..1081
                     /locus_tag="PWU81_002558"
     CDS             158..1081
                     /locus_tag="PWU81_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL1111224.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1087..2340
                     /locus_tag="PWU81_002559"
     CDS             1087..2340
                     /locus_tag="PWU81_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL1111225.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2474..3820
                     /gene="gor"
                     /locus_tag="PWU81_002560"
     CDS             2474..3820
                     /gene="gor"
                     /locus_tag="PWU81_002560"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL1111226.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(4755..5708)
                     /gene="menA"
                     /locus_tag="PWU81_002561"
     CDS             complement(4755..5708)
                     /gene="menA"
                     /locus_tag="PWU81_002561"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL1111227.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(5721..6824)
                     /locus_tag="PWU81_002562"
     CDS             complement(5721..6824)
                     /locus_tag="PWU81_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL1111228.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(6914..7870)
                     /locus_tag="PWU81_002563"
     CDS             complement(6914..7870)
                     /locus_tag="PWU81_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDL1111229.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            8151..10085
                     /locus_tag="PWU81_002564"
     CDS             8151..10085
                     /locus_tag="PWU81_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL1111230.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            10542..10958
                     /locus_tag="PWU81_002565"
     CDS             10542..10958
                     /locus_tag="PWU81_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL1111231.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            10972..11538
                     /locus_tag="PWU81_002566"
     CDS             10972..11538
                     /locus_tag="PWU81_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDL1111232.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            11561..12547
                     /locus_tag="PWU81_002567"
     CDS             11561..12547
                     /locus_tag="PWU81_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL1111233.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(12702..>13226)
                     /gene="scm"
                     /locus_tag="PWU81_002568"
     CDS             complement(12702..>13226)
                     /gene="scm"
                     /locus_tag="PWU81_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL1111234.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         4118 a         2400 c         2726 g         3982 t
ORIGIN      
        1 tttttgtcac aatctctttt tatatttgga cagaatatat cgaactttat tttgattttt
       61 tgtgaaataa ttttcaaaaa tccgacttta aatagaagaa gtattggaaa gtttttagag
      121 ctatgctact attatgaagt tgtaggagga gaaaattatg atcgaactca taaccacagt
      181 agaaactatt gaacaagcag aacagttgct tgctgcaggt gcagatacac tatatttcgg
      241 tgaagagcaa tttggattgc gtctgcctgc ttcatttaca cgagaagaac agcgacaatt
      301 ggtagaatta gcacacaagt atggtaaaaa agcgaatatt gctgtaaatg ggatcattca
      361 tcctgaaaaa atgaaaaatg tacctgaata tttggctttt ttaaaagaaa taaacgtaga
      421 tgtgatcaca gttggtgata caggtattat ttatacgatg aaaaaaaatc ctgaattgcg
      481 tataccgtat atttatgatg ctgaaacatt agtaacgagc gcacgtcaaa tcaatttctg
      541 ggcgaaaaaa ggcgcagttg gagcagtact tgctcgtgaa gttccttttg aagagatgaa
      601 ggcgatggaa gaaaaactcg atattcctgt agaaaccctt gtgtatggtg ctacatgcat
      661 ccatcaatcc aaacgtccac tgctacagaa ttactataac tatacgaaac aagacgaaca
      721 aaaagatcgt gaaagaggtc tttttatctc ggaacctaaa aaagaagaga cacattattc
      781 tatttatgaa gatagtcatg gcacacatat ttttgccagc aatgatctga atctctcgaa
      841 tgaattgaat gaactttatc agcatcatta ctgtacatgg aaattggacg gattatttac
      901 tccaggcgaa aaattcgtaa acatcactcg actattcgcc gaagcaaaaa aacaaatttt
      961 agaaggtagc tgggatcaag aaaaagcgga aaaaatgact agcgaaatca gtaaattgca
     1021 tcctgaaaac cgtgaattag atacaggatt cttttatatt gatccttctg ctatcaaata
     1081 aggagtatga tgaaagaaat gacagtaaaa agaaaattga aacgaccaga aatcctagcg
     1141 ccagcaggaa cactggaaaa gttaaaaacc gcgatccatt atggagcaga tgcagtatat
     1201 attggcggga atgcatatgg tttaagaagc cgtgcaggta attttactta tgaagaaatg
     1261 gctgaaggag ttgcttttgc aaaagaacat gatgcgaagg tatacgttgc tgctaatatg
     1321 gtcacccatg aaggaaatga agatggagca ggggaatttt tccgcagttt gcgtgatgtc
     1381 ggtatttctg cagtgattgt ttctgatcca gctttgatcg aaatttgtgc gacagaagcc
     1441 ccaggtctgc cgatccattt atccactcaa gcttcagcaa ctaattttga gacattggaa
     1501 ttctggaaag aagaagggtt ggaacgtgtc gttttagctc gtgaagtttc aatggaggaa
     1561 gtggcagaga ttcgtaaaaa tacggatacc gaaatcgaag catttatcca tggtgccatg
     1621 tgtatctctt attctggtcg atgcacattg tccaaccata tgtcgatgcg ggatgcaaac
     1681 cgtggcggat gttctcaatc ttgtcgctgg aagtatggat tgttcgatat gccttttggt
     1741 caagaacgaa acagtttaac tgatacaggc gaagtggaag aagaattctc gatgagtgca
     1801 gtggatatgt caatgatcga acatattcca gaattgattg aaaacggcgt agacagcttc
     1861 aagatcgaag gaagaatgaa atcgatccat tatgtatcta cggtgtcaaa tgtctataaa
     1921 gcggctgtag acagttatat ggaagatcct gaaaactatg tctgcaagca agaatggatc
     1981 gacgagttat ggaaagtagc acaacgagaa ttagctacag gtttttacta caatacacca
     2041 actgaaaacg aacaattatt cggtgaacgt cgtaaaatcc cagtatataa atttgtcggt
     2101 gaagtattat cttatgatga ggcaacacag attgctacga tacgtcagag aaacttgttt
     2161 tctgtaggag atgaaatcga attttatggt ccaggattca gtcatttcag tcaaaccgtt
     2221 gaagttatgc ataatgaaga aggcgaatcc attgaccgag caccaaatcc aatgatgatt
     2281 ttaactatgc cagtcgcaca gccggtgaaa cctggagata tgatccgaaa acgtaaataa
     2341 ggaaaaagct gatatatccc tgttttaagc atcttcgcat acaaaattca agaaaagtgt
     2401 tacacttgtt ttctgtagaa aaaggatcgt taaataaagg ttttgattga tctcttcaat
     2461 aaggaggaac aaaatgcaaa tatatgatta catcgtaatt ggtggcggct ctggcggtat
     2521 tgcttcagca aacagagcag ggatgcatgg cgcaaaagtt ttgctgattg aagcgaatga
     2581 attgggcgga acatgtgtca acgtcggttg tgtacctaaa aaagttatgt ggcaagcaag
     2641 tacgattttg gaaacaattg aacgagatgc ctcaagttac ggtatccaag cagatttaaa
     2701 aaatgttgat ttcgccggtc tagtagaaag acgagaaaaa tatattgatt ttctacacgg
     2761 tgcttatcaa agaggattag atagcaatca tgtcgaggct ataaaaggtt acgctacttt
     2821 tatcgatagt caaacgatcg aagtaaacgg tgaaacctat cgtgctccta aaatcttgat
     2881 cgctactgga ggccgtgcaa gtaaaatgac cattcctggt ggagaatacg caattgattc
     2941 aaatggattt tttgaattaa cagagttacc taagagagca atcgttttag gtgctggata
     3001 cattgcggct gaactttcag gtgtatttcg tggtttaggt agtgaagtca tgtgggcata
     3061 tcgaaaagaa cgaccactta gaacgtttga taaaatgctt tcggacaatt tgatccaaat
     3121 gtaccaagaa gctggaataa aaacttacgc acatcatatt gctaaagaaa ttactaaaaa
     3181 caatgacgaa tatacggtgc tatttgaaaa tggtgaaacc ttaactggtg attgtgtact
     3241 ttttgccggt ggacgtgtac cgaataccga aaaattaggt ctggaaaata caaatgtgga
     3301 attggacaaa aaagggttta tcaaagtaga taaataccaa aatacaacgg atgaacatat
     3361 ttttgctgtt ggtgatgtga tcggtaaatt agatctgact cctgttgcaa tcgcagctgg
     3421 tagacgttta tcagaacgtt tatttaatgg aaagaaagat tcttatcttg actataaact
     3481 tgttccgaca gtcgtcttta cgcatccacc aattgcaaca attggtttga cagaagaaga
     3541 agcattggaa aaatatggcg aaaatgaatt gaaagtttat cgttcacgct ttaccccaat
     3601 gtactttgca ttaaatgatt atcgccaaaa atgcgaaatg aaattgatct gtgtcggaaa
     3661 agaagaaaga atcgtcggct tacatgctat tggtattggt gtcgatgaga tgctgcaagg
     3721 ttttgccgtg gctatcaaga tgggtgcaac taaagaagac tttgacaata cagtagccat
     3781 ccatccaaca ggagcggaag aattcgtaac gatgcgataa tttaatatta acagttccaa
     3841 cagcttttta ttgaacaaac catgtaaaac cagaaaatca gtatactgat tttctggttt
     3901 tttgtttttt agttatttat gtaagattgc ttcgataatt ctcaaaacga aataagtagg
     3961 gaagttggag attttgatta tcttcgtaac tgacgaagag taaatgaatc catttggtat
     4021 gcactcttaa gttcaaaata ttggtatcga tgtttaaaag aaatagaaat gtatcgaaga
     4081 acagatgttt aaaaaagtgt gactttgtat aaataaatta gaagaagcaa acgaggatgt
     4141 taaaagaata ccatatagtt accaaaccaa gaaagttctc gtcaagttcg ctggtacgaa
     4201 tcaaatcaag tgaaaaaagg gactgtgaaa aaaattgtgt catagttagt cactttttcc
     4261 gaacaaatag tgttcaatca gctgtccttc ggtattagct caaaaaacgg caaaatatga
     4321 ggaactattt ttccatttgt ttctctaata ctcaggacaa aacactgatt ttacaacctc
     4381 tgataaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgtg
     4441 gaaagccatt ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct
     4501 ctgataaacg gtgttcaaaa gccagtttct cggtattaag ccaaatttat gcaaaaatgt
     4561 ggaaagccat tttcacaaaa tttttcttaa tactcgaaac taagcgactt tttcacaacc
     4621 tcctgataaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc aaacgaaaaa
     4681 tataaggagc tatttttatt tggctgttct tattacttga tgcagggcag ttttttcaca
     4741 acctcttcat gctgttaaat ggcttgcata atgattgtta gtaatagccc caatacataa
     4801 ctggcattga ataagaccat atttttgatg gaatagctga aactgatagg ctggggtaac
     4861 gtttgcttga attgatccaa atgcttcttc acaattggca aggtcaagaa ggtgaccaag
     4921 atcggccatt catagatacc gctcaacaac cccattaaaa tcacgaaata gcaagcatac
     4981 atcaatagct ggaataagac tgttcccgca ggacgtccga tataaaaaac tagtgtatag
     5041 cgatgatttt caatatcacg gtcaagatcc cgcaaattat tagccaacat gatattggca
     5101 atcgtaaaca ccaagggtaa ggaagctaat acgattgctg ctatagccca aagatttcct
     5161 accaaagcaa attgtccaga tgaaaagttg actgtcagat catagacatc tgaaccgaat
     5221 gtattgacat aaacgaccat ggcaaaaatt ccaaggccca tagtcacccc actgaaaatt
     5281 tcccctaaag gcattcgaga taaaggaatc ggtccgaatg tatagaatac tccaataaaa
     5341 caacagataa atcccatcaa aagtaaaagc caacccgtat tgatcgttag aataacgccg
     5401 ataacaaaaa caaatgcaat catagctaaa atcatgtttc gaatcaatac aggagataga
     5461 tttctgcggc caatgacatt ttcttcatac ttatatactt cagaatgagc cttttcataa
     5521 tccatgtaat tattgatagc ggtagtcgct aaatcaaaaa ctagcattcc aataaaaaat
     5581 aaaaaagtgt ttagccaatg tatctcatga aaaaaagcga tagagaataa tacccctacc
     5641 gcaaaaggaa atacactagc cacctttgtt cgtatttcaa ctacttctaa aaagattttt
     5701 agtgtcatgg ttcgtactcc ttattttagt tcagaacggt cacccatcgt gtaatgggag
     5761 tcctccgata atttgaaggt gtctttgatt ggatcggtca catatacttt atcgtctttt
     5821 gtcacgaaga tcgcttccgt cccgttattc aattcgcttt cgacgtattc cagtcctttt
     5881 ttcacaccca ttgaaaagac tgctgtagac aacccgtcac catcaattga tttatctgtt
     5941 atgatcgtga cacttgcgat atcattgtcg aacggataac ccgtttttgg atcaaacaaa
     6001 tgatggtatt ttttgccatc tacttctaaa taacgctcgt aaattccaga ggtaactaat
     6061 gttttgttcc gttctttaat agaacctaaa acagatccgc gagccatgtt cggatcttgg
     6121 atgccgactg tccagtcttg gttttcgcct cttgggctgt ggcctaagac atagacatta
     6181 ccgcccaagt caacgatggc agtggtcact ccttgttttt tcaagacttt cactacttcg
     6241 tccgtaatgt aacctttggc gattgcacct aaatcgatga tcattccttt ttcttttaga
     6301 taaacagttt tttctttgtc atttagttcg atcttatgat aatccactaa tttcaatgcc
     6361 tgatcgattt cttcttggga aggttttctg gcgtcatcaa aaccgattcg ccataattgt
     6421 gtgattgcac caatcgccat atcgaatccg ccttgagaat cttggctata ttcgtaggcc
     6481 cgcttgacta acgtgtagac gtcatcagaa acttttactg gtttgatccc agcttgctca
     6541 ttcacttcat cgatctcaga acctttttga ttgatcgtga ttttatcacc aagttctttt
     6601 actcgatcga aggcaggttt caatgctgat tctttgcctt catcatacac acggatacga
     6661 acgtaagtcc ctaacaagaa ctgttcatca gaatatggat ctttcaatat cttggtagct
     6721 gctgaacttt cagtggttgt tttatttgct gagttgctgc agccagttag caccataaaa
     6781 gagatgccca gtgtgattaa ggaaaatagt agttttttcc gcatcttttt cctctcttca
     6841 ttttgaaagg tgagacgact gcctaatctg gctatcgtcc caccctccaa cttttattag
     6901 tatgattatg tgattatttt tctgttacga tattatctac ttcgattgtt tgagtgtcgc
     6961 ctttttcagc tgcattcact aattgttgtg cgtacatgat gaatgaatgt gagctgtgtg
     7021 tagcacctgt aacaacttct acattagcag gtgagccatc ttcttcgccc attgctttga
     7081 cgaattcgtc gttcagagta gggatgtaag tttttggttc tgtaccgctt tttgctttca
     7141 tattttcatt atattctgta tcatcttgtt ttgatttacc gtctttgtcg atataatcaa
     7201 agtttgattc agttactttg ccgccagcaa ctgtcatttc gaattgcaca cggtagccgt
     7261 tagagtagtt cttttcttca agcttgtaag tgccgtcttt caaatcagca ccattgtcga
     7321 tttcgattgt atctgtgtta ccagcttgag cagcttggat caattgttgt gcgtagtttt
     7381 ggaagctttc agatgagtga gtagctccag ttacgacttc cacaccgctt gcgctttgtg
     7441 cttttaagaa ttggtcattc aactcaggaa tgtatttttc tgggtttgtg ccagattttg
     7501 cttccatgtt tttgttgtat tctgcatctt ttgtttttga ttcgccgttt tcattgacgt
     7561 tatcatattt tgattcagtg attttgccgt cttttacaac catttcaaag actgcacggt
     7621 aaccatttga ataatttttt tcttctaatt tatatgtgcc gtcttgcaaa tctccgccag
     7681 ctacaacttt tgcagatgat tctgttgtgc tttttgcagt cgaagattgt gctgtagatg
     7741 attcagaaga agagtttgag cttccgttgt catctgcgcc gcaagcgcct aacactagag
     7801 ctgaaaatgc taatacggca aatccagtta caaaacgttt tttcttcatt ttaatacccc
     7861 accattccat ttctagtcca caatttttct ttgtgattca ttatacattc taatcttttt
     7921 agcctataag tcaactattt ttgataaata acccatgtaa tatttcaaac gttttctttc
     7981 agcaaatttc gtgaaaaaac gctttatttt aaaatttgaa aaaaatccaa aaggagttag
     8041 acaaaagaac agaacatcta tataatatat ggagattgtg taataagtat caaactaaaa
     8101 gaattatcat atattcatta tatagatagt ctataaggag cggatgatca atggcaaagc
     8161 aaaatattgt cgttgtagga gcaggatatg ccggtgtttc agctacaaaa tacttggcaa
     8221 agaaactgaa aaaagaagat gtagcaatta ctctgatcga tcgtcattcg taccatacta
     8281 tgatgaccga gttgcatgaa gtagctggcg gacgtgtaga accagaagca atccaatacg
     8341 atctgcagca tttgtttgct cgtcaaaaga atgtacaact tgtcacagac actgtaatcg
     8401 gtatcgataa agaacgaaaa atcgtcaaga ccaaattagg ttcctatcct tttgatcagc
     8461 tgatccttgg aatgggcgga gagccgaatg actttggtac acctggtgtc aaagcacacg
     8521 gattcaccct ttggtcattt gaagattctg taaggattcg ggaacatatt gagaagacag
     8581 tagcaaaagc tgcattggaa ccagatgatg aactgcgtaa agccatgctg actttcgttg
     8641 tctgtggttc tggatttacc ggtattgaaa tgattgggga actgatcgac tggaaaaatc
     8701 gcctagccaa agattataaa ttagatccaa acgaaatcac actgatggtc gtagaggcaa
     8761 tgccgacgat cttgaatatg ttggatcgaa atgatgctgc aaaagctgaa cgctatttgc
     8821 gtaagcacaa cgtggaatta ttattgaatg caccaatcgt agaagtggct gctgatcata
     8881 tcaaattgaa agacggacgc aacattccga ctcacacact gatttggaca gctggtgtca
     8941 aaggaacttc tgacgctgct gactttggat tagaatcagc acgcggacag cggttgatcg
     9001 ccaatgaata tatgcaggca aaaggctacg aagataaaaa catctatatc attggtgatt
     9061 tagtctttta tgaagaattc ccaggaacac cgacaccgca gatcgttcaa gctgctgaac
     9121 aaacaggaca cacagccgca gcgaatatcg tggcttcgat caaaggcgga gaaaagcata
     9181 aatttaaagg aaactatcaa ggatttatgg tttctattgg tgccaaatgg ggggtagcta
     9241 acctatttga taagatccat ttaagcggat ttttagctat cgtaatgaaa catatcgtga
     9301 atttgaaata cttctttgat attcgttctg gttattatat gttccaatat ttaatgcatg
     9361 agtttttcca tatcaaagat gatcgcaacg tgactcgagg acattcttct cgttacggca
     9421 atgtcttatg gagtgtgcca cttcgtattt tctatggttt agtttggttc gttgaagctt
     9481 ccaaaaaaat cattgggaat ggtgaagtgt tcaaaccaag tacttggttt ggtgaaggat
     9541 catggttcac tggaaacagt gtgttccctt ggacatggga atacaatgta acagatgttg
     9601 ccagtggtgc ttctgctgct ggtgatgcaa ccacagctgc cagcggagca ggggcaagtg
     9661 ccggagaagc agcgactcaa gctgcacatt ttggtttgag ttatgcatac ggtgaagaac
     9721 caatggctgt gattgagaag tcaccagact ggttcacaaa gatcatgcaa gtgatcatgc
     9781 caaacaatga tgtagcaatg ttcttccaaa aattcatggt attctttgaa attgccttgg
     9841 cattagcatt gattgctgga ttattcacat ggctatgcag tgcgacaacg attgtattgg
     9901 tgatcacgtt ctgcttatca ggcatgtttt actgggtaaa catttggttc atttttgttg
     9961 ctttcgcatt gatgaacggt tccggacgtg cgattgggtt ggatcgctgg gtaattccat
    10021 ggctacaacg tacactaggt aattggtggt acggtagacc aagttcccag tacggcaaga
    10081 aataaataga taaagaacgt tatttgaatg caagatttca atagaaaggg gctgagacaa
    10141 accgtcacag cctccttttt atgtaaaatt aaaggaataa gatgcccgag ataagcctcc
    10201 atgagcaaga cacaggtgtc acaacctctg ttacaacctc ttgttataaa cggtgttcca
    10261 gaagtagctt cttcgaaaaa taagccgaaa tttcacaaaa acttgaaaag taattttcgt
    10321 aaattccttc ttatttcttg aagttgccca cttctgtcac aacctcttgt tataaacggt
    10381 gttccacctg ctgcttctcg gtaataagcc caaagaatta gaaaacatgg aaagctgttt
    10441 tctattcttc gttcttatta ctcaaagcaa gacacaggtg tcacaacctc ttgtttttat
    10501 gctaaaattg ggtgagttaa tctggaaaaa ggagaacaaa aatgagcctt aaagaatttg
    10561 ctgaaaaaag tctgctcaaa ccttgggatg tttttattat tttgcttttg actgttcttt
    10621 cttttttacc agtcatgatt tttggtcttc agcaaaacac cgcaacagca gaaaaagaag
    10681 cggtactgcg tgtggatggc acagaaatca aaacattccc tttgatggct ggagaagaga
    10741 gcaacacata tacttacaaa gatgaacacg gagattataa cttgatcgag atagatggcg
    10801 atcgagtacg gataaaagaa gcagattgcg atgaccagat ctgcgtacgt cgaggctggg
    10861 caacgaaaaa tggcgaaacc atcgtctgtt tgccacataa attagtgatc gaagtccgtt
    10921 caacagatgg aggtgacgaa gacagtctga tttattagac cttcacacac catgagtaaa
    10981 ttacaaaaaa tgatttatat ttcgctactt gttgcacaag gagtgatcat tggtttattg
    11041 gaaaatatga tcccttatcc tttttctttt gcgcctggtg caaaactagg cttagccaat
    11101 ctaatcacga tcgttgccat ttttaccatg aaaaaacgag acagcttctt actactttgg
    11161 ctacgactat tcctgactac tttgctaggc ggaacgattt ccacattcct ttatagtatg
    11221 agtggtgctt tgcttagtta ttttggaatg ttattggtca agcaattagg accaaaacgt
    11281 gtcagtatca tcggaattag tgcggccggc ggtttcatgc ataatgtcgg acagcttttg
    11341 atggcttctt tcattgcaca atcatggaca gtcatgttgt acttgccagt cctttcagtt
    11401 ttagggattt tatcagggat tgcaatcggg atcgcagcga attatctttt acgacatgtg
    11461 cagacacttc aacgattcca aaaagattat gagcagggaa ccaaacaaaa atggagccca
    11521 atgtttacca aaaaataatc gtcacacaag gagttttttt atgaagctac atccaatgtg
    11581 ggaaaactat cctgctttaa gtaaagaatt gacagaaaca ctaaaacgga tggaagaaac
    11641 tgtccgcctg aaaaacaaac ctgtcgaaca agcaatcaaa gaaacgatcc atgccggcgg
    11701 aaagctgttg cgtcctgcct atcagctatt gttctcacaa tttggaccag agcaagatcg
    11761 agacaaagcc atcgctttgg cagcttctat cgaaatgctc catacggcca cactgatcca
    11821 tgatgatatc gttgatgaag cggatctgcg acgtggtcag cctaatatcc gtagtcagtt
    11881 tggtaatgtc gtttctgtct atgcaggaga ctatttattt gtctgctgtt ttaaactatt
    11941 gtctgattac tccacttcct tgaaaagtct ccaattgaat tctcgcagta tggaaaaagt
    12001 attgaatggc gaattagggc agatggatga tcgctataat tatgaacttt ctgtaaaaga
    12061 atatttggaa aatatttcag gtaagacagc tgaactgttc caattaagtt gttctgttgg
    12121 cgcttttgaa agtggaacaa gcgagcgctt cgcaaaaaaa gcaggagata tcggactttc
    12181 tatcgggatg gcctttcaga ttatcgatga tattttagat tatacaaaag agagccagga
    12241 aatcggcaaa cctgtactag aagacatgcg tcaaggggtc tattctttac cgttgatcta
    12301 ctctttgcaa aaaaacaaac caaagctact accttattta gagaagaaag cggcaatgac
    12361 agaagaagat gtcgacgccg tacgcaaaat cgttgaacat acaggaggcg tggaagaagc
    12421 taggaagtta gcggcctctt acacagaaaa ggctttgaag gaaataaaga aattacctgc
    12481 cacctctctt cgaacaaaag agaatctctt ttcccttacc caactgattt tggatcgcaa
    12541 agactgaata gggaaaggtt cgatacgtaa ataaatttct cagaaggaaa tctacgtgtt
    12601 ttttgacaaa ttgaattgta gttcttgtat ttatgataaa aagcaaaaat aaagagcaac
    12661 gtccgttagc gacgttgctc ttttttatct tgaatatttt tttacctttt cgatttctta
    12721 taactcataa aagtgattct taaaccgatt acaagtaata taacgcctgc gagtgttatc
    12781 caattattcg attgacttcc agttttagga agtgctcttt ttgatttgtt tccattgtca
    12841 ttgcttgttt tattagttaa gttaaagttc gtcctgttat ccgaacttgt ttttgagaag
    12901 gagacacttg catgacgtgt atcgcttgtg gtagacgacg tgtctgtact atcgcttgaa
    12961 gttgatgaat ctgagctatc acttgtggta gaggatgtat ctgtactgtc acttgaagct
    13021 gacgtgtccg tgctatcact tgtagtggta gatgaatctg agctatcact tgtggtagag
    13081 gtagtatctg tactgtcact tgaagctgac gtgtccgtgc tatcacttgt tgtggtagat
    13141 gtatccgtac tgtcgcttga agctgacgtg tccgtgctat cacttgtggt agaggtagta
    13201 tctgtactgt cacttgaagc tgatgt
//
LOCUS       DANJMI010000071        13085 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000071 DANJMI010000000
VERSION     DANJMI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.071"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(79..2199)
                     /locus_tag="PWU81_002569"
     CDS             complement(79..2199)
                     /locus_tag="PWU81_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL1111235.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="PWU81_002570"
     CDS             2429..3607
                     /locus_tag="PWU81_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDL1111236.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="PWU81_002571"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="PWU81_002571"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDL1111237.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="PWU81_002572"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="PWU81_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDL1111238.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="PWU81_002573"
     CDS             complement(4960..6213)
                     /locus_tag="PWU81_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL1111239.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="PWU81_002574"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="PWU81_002574"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL1111240.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="PWU81_002575"
     CDS             7790..9136
                     /locus_tag="PWU81_002575"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL1111241.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="PWU81_002576"
     CDS             complement(9444..9830)
                     /locus_tag="PWU81_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL1111242.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="PWU81_002577"
     CDS             complement(10098..10247)
                     /locus_tag="PWU81_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111243.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10709..10921
                     /locus_tag="PWU81_002578"
     CDS             10709..10921
                     /locus_tag="PWU81_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111244.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="PWU81_002579"
     CDS             complement(11199..12998)
                     /locus_tag="PWU81_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL1111245.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2353 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 tttaa
//
LOCUS       DANJMI010000072        12738 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000072 DANJMI010000000
VERSION     DANJMI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.072"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            97..540
                     /locus_tag="PWU81_002580"
     CDS             97..540
                     /locus_tag="PWU81_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL1111246.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(546..1730)
                     /locus_tag="PWU81_002581"
     CDS             complement(546..1730)
                     /locus_tag="PWU81_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HDL1111247.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            1998..2696
                     /locus_tag="PWU81_002582"
     CDS             1998..2696
                     /locus_tag="PWU81_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL1111248.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2703..3380
                     /gene="nth"
                     /locus_tag="PWU81_002583"
                     /pseudo
     CDS             2703..3380
                     /gene="nth"
                     /locus_tag="PWU81_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(3506..4039)
                     /locus_tag="PWU81_002584"
     CDS             complement(3506..4039)
                     /locus_tag="PWU81_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111249.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4336..6717)
                     /locus_tag="PWU81_002585"
     CDS             complement(4336..6717)
                     /locus_tag="PWU81_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL1111250.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6707..7354)
                     /gene="recU"
                     /locus_tag="PWU81_002586"
     CDS             complement(6707..7354)
                     /gene="recU"
                     /locus_tag="PWU81_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDL1111251.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7418..7957
                     /locus_tag="PWU81_002587"
     CDS             7418..7957
                     /locus_tag="PWU81_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HDL1111252.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8047..8466
                     /gene="gpsB"
                     /locus_tag="PWU81_002588"
     CDS             8047..8466
                     /gene="gpsB"
                     /locus_tag="PWU81_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDL1111253.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8488..8855
                     /gene="rnpB"
                     /locus_tag="PWU81_002589"
     ncRNA           8488..8855
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PWU81_002589"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9064..10230
                     /locus_tag="PWU81_002590"
     CDS             9064..10230
                     /locus_tag="PWU81_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDL1111254.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10276..11772
                     /locus_tag="PWU81_002591"
     CDS             10276..11772
                     /locus_tag="PWU81_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HDL1111255.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11853..12536
                     /locus_tag="PWU81_002592"
     CDS             11853..12536
                     /locus_tag="PWU81_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL1111256.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         4272 a         2230 c         2630 g         3606 t
ORIGIN      
        1 tcttggtttc agattgggtt ttcctacaaa atatttttat ctttgttgaa taggaaacaa
       61 aaaaacaata taatgaaaga aaaagaaggt gtagaaatgg ataaaagaca actgctaata
      121 gaagaatatc tggatttagc taatgagatt attcatcgta ataaaccaga aatggaaaaa
      181 gaatttgcag gatttactct aaatgaacta gaagtgattg aacatatcgg gaaaatccct
      241 gatcctaacg tgacaaaatt agcgaatgct agttacatga ctagaggagc catcagtaag
      301 ctaacaaaaa aactgatagc tagagggatc ataaacactt atcaaagcga agagaataaa
      361 aaagaaatct attttcaatt gacgccaatc ggacaaaaaa taaggaatag acacgagcaa
      421 ctccatcaaa aatgggtaca gcgagatgct actgtatttg aaaatatgtc aaaagaagaa
      481 tttgacacag tatttcgttt tatcggtcga tacagagaat ttatacgaag tagcaaatga
      541 agctactatt ttttattgta gtgataatag gcaaaaatca taagcaaaaa tccgaatacc
      601 gtcggaagaa atggtgctgc agcataaagc tgtcctgcga ttagcgggcc gatgacgcgc
      661 gatagagatt gtatactttg ggtaccgccc attacaagac cttgctggtt atctgagaca
      721 gaattggata gttggccatt gaatacagga gtaaaaatag aatcaccgaa accataacag
      781 ataatcccaa ataaaaacaa aatcggcagt agaagaattc cagataatgt gatgaataga
      841 taggcgagga tttcactgaa catccctatt cttgtgatct gctgttctga aaattttttg
      901 agcaatcgag gcataatgaa caattgcgag acagtatcca agattccaat caaagagaag
      961 cttaaaccaa ccaacccagc cttccactga aaggcgtcaa tcgaaaattg agaaaagata
     1021 gattgaagag agccagctgc caaccaaaca gcaaaacctg ctgtcaataa aggaataacg
     1081 gatgaaatct tgaataattg ttgtaattgg tgaaaaggtc ttacatggga gaaacttaaa
     1141 tctactgaac gtttttttat tggaaggctt tctagcataa atgtgtagcc ataaacggca
     1201 tttaagaaag taaagagtgc tccaataaat ataggaacag aattccccaa ctcagctaat
     1261 agacccccta taataggacc cagcgttgtt cctataccga ctaaagcacc catccatcca
     1321 aaaactttgg ttctttcatt aggttccgtg atgtcggcaa agtaagcata taaggtggag
     1381 atttctcctg ccgtcaatcc ttcaatgata cgaccaagga acaacatcca tatcgaattt
     1441 cctaaaccaa aaagcaaata tccgatactt gatcctaata aactgataat aaggatcgga
     1501 tgacgcccaa aatgatcact taagctaccg agaatggggg cagataagaa ggcagctaga
     1561 gcataaacag acatgagcaa agtgatatag aaagcttgct gatgtatgga gtgagaataa
     1621 ggcagcgtta aaaacggaag tacaggactg acgatagtaa atcctaatcc agtgaataag
     1681 acagagacgt atccaaaaac tttagaatgt ttatttgtga aactgtacat gtaaaaactc
     1741 ctttatgcaa taatgttaac ttggaaacaa aaatagtgta tagtaatttt gtttccttgt
     1801 caacattcta aggaacagcg aaatctactt aatttattta ttccgaaaag tgaagatacg
     1861 catcaaaaac atgaacaaac aaaaatcaag tagacgacat tgtaaatctg accgatcagt
     1921 gaagtaaata gaaaagaaac ctgttcttgc ttcccatgtt ttttttacgt atactgtgat
     1981 tgagcggagg agaaatcatg ttgaatttag aaaattactt aagcgctgga caaacagtca
     2041 tatctaatct tttacttaaa aattatcata ggatcggtct acaatctgaa gaatttttat
     2101 ttgttctaca gttgcatatg gcacagcttg aaggagatac tttccctgat ttgcagatga
     2161 ttgctatgga tatgggaatc aaacaagatc agatttttca aatactggat cgtctcgtaa
     2221 caaatggatt tatcaagata gaaacgacga ttgtcaacgg taaaaaagca gatcgatata
     2281 atctttaccc catctatgat ctattaggtg agtatttaaa aacacaagaa aaaaaatacg
     2341 aagaaaaaac acaagaaaaa gaaatccagt cagtgtatca attatttgaa caagaatttg
     2401 gacgcccgct ttcttcgatc gagttccagc ggatcggaca atggctagaa gaagaccatt
     2461 atcagccgga aattttgaag ctagcactgc gggaagccgt attaaatcag gcttatagtt
     2521 ttaattacgt tgaccgcatt ttgctatcgt gggaaagaaa aaatctacgt acaaaacaac
     2581 aagtggaaga agaccaaaaa cgaagaaaac agcagcttct tcaaagagaa gtagaacagt
     2641 cagctcaaca agaagaattg ccgaaagtct cactgaaaaa ttggttggag gagtagcgga
     2701 atatgttaag caaacaaaaa acaatggaag ctctagaaac gatgtatgga atgtttcccg
     2761 aagcacatgg agaactcaag cacaataatc catttgagtt attgattgca gtgatcttaa
     2821 gtgcccaggc gacagatgta tcagtgaata aagcaacgcc agatttgttt gcctcttttc
     2881 cgacacccga tgcgttagca gaagcttcaa tcgatgaaat cattcttaaa ataaaaacga
     2941 ttggtttgta tcgaaataaa gcaaaaaaca tcaaagcgtg tgcacagcag ctgattgaac
     3001 gtttcgatgg gcaagtgcca acgtcaagag aagaattgat gtctcttcct ggtgtaggca
     3061 gaaagacagc aaatgtcgta ttgggggatg cattcgggat accagcaatc gcagtagata
     3121 cccacgtgga acgtgtctca aaacgcctga ggatctgcaa actagatgct acggtcatgg
     3181 aagtagaaga aacactgatg cggaaagtac cacaagaatt atgggtaaag acacatcata
     3241 cactgatttt ttttggttga tatcattgca cggcaagaaa tcccaagtgc gaggtatgtc
     3301 ctttgctatc aatatgtcaa gatgggaaaa atcggatgcg tctaaaagaa aaggcgctaa
     3361 aaaagaaacc acgcctgtag acgtggtttc tttaattgca aataaatgta tgaattatct
     3421 tgttattatg gagaaaagct gtttcgttta tcttttttgc atgaaaaagt aaatgtacat
     3481 actcagtagc taggatggta acttattaca atttattgcg ataaaaataa aagttgttat
     3541 ttttattggg aaaatacccg atgtagaaaa taatgccttc caatatacct ataaaataag
     3601 tccagtattc caaaggatta tataaataag tgacgtatct atctaaaggt gtaataaaaa
     3661 tgataagtga taataaaaaa ataccattta taactaaaca taaactaatg gatttgggcg
     3721 gtttcaggct catcagaaaa atatagattc tggtgaacaa caaccataaa aaagtgagaa
     3781 tcattgcatg agagcttctt tccataaatt ttcctttaag agtagtgctt aaaagtacag
     3841 aaaaaataat aaatgcaagg ataggagcaa ttaaagtaaa tttatttaat aataaacaaa
     3901 atttttcagc ttttttattc ttggaataac gaggtattgt tttcgataaa aaaagatggt
     3961 gcagacaaat tatgataatt aagcatacta tcagataact caaaatgaag ccttctttca
     4021 aaaaattatt caaaaccata tattgtattt tttatttgat aataacaatg ggattattaa
     4081 gaagtggcac taaataaaag actagtgtgt atttagccga tttaattata aacaagtaaa
     4141 tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta agctaaatgt
     4201 tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac agcttggtac
     4261 atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac gcttgtagac
     4321 gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg gtgtatggtc
     4381 atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag gagcagatga
     4441 agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag acgctggctc
     4501 gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc tttgactagt
     4561 agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct ggacatagct
     4621 ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat attgatcttt
     4681 gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt ctgtatttgt
     4741 cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta cgtgtgacga
     4801 ttctgaagtg acaggcgtca tttttttatt gtaacctgtc cacacggaaa tcgaataatt
     4861 tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac caagttttgc
     4921 atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata acccagcgat
     4981 ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg tgatcatata
     5041 agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt ctgttccgtc
     5101 ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat tggcgaaagc
     5161 cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat cttgttcttc
     5221 cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc caagattttt
     5281 taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg ctggaacatt
     5341 acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat tatcccagtt
     5401 tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag tttttccagt
     5461 agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca tcgtagaacc
     5521 aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt ctgcgatgtg
     5581 acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg tggaggcaac
     5641 ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat catatagttt
     5701 cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt agacattctt
     5761 atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga cgatcttagt
     5821 attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat taacagcttg
     5881 atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa cagtatctcg
     5941 tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg cttgaggcat
     6001 cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac caaagtacat
     6061 ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat ttacgtagta
     6121 ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc acgcttcttg
     6181 ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa gtttgatcaa
     6241 ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag agagggcaga
     6301 accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat cttctaccga
     6361 cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt cacgcttttc
     6421 tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag atacggtagc
     6481 gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga aaagtcctac
     6541 acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct tgataaataa
     6601 tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct tcttcacact
     6661 ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat ttgccattct
     6721 tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc aaataaggaa
     6781 tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa gataaaggta
     6841 gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga acactattca
     6901 aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc ccattttgtt
     6961 cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag ggaaaagatg
     7021 ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag acgccattat
     7081 agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca cttctcttag
     7141 gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc gccaacccgc
     7201 gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc ccccgattgc
     7261 cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag gcattcttat
     7321 aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct aggccacatt
     7381 atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa aagtaatcta
     7441 tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc ctaaaatcca
     7501 agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag ggctggaatg
     7561 ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag tagcggaatt
     7621 gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg attttggtaa
     7681 taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag cagattatgt
     7741 cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc acactcgttt
     7801 tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc ctggaaaaac
     7861 gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg aaattcggct
     7921 gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt tgatttatca
     7981 acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa aactagaggt
     8041 gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga attcaaaaca
     8101 aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat catcaaagat
     8161 tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg attgagtgca
     8221 aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca acaaacagag
     8281 gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc aaatcttgaa
     8341 cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc agcacaacct
     8401 aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa aacacgtcaa
     8461 ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga cttgatcagg
     8521 ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc ttagcgaaac
     8581 cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct tcggctatgc
     8641 ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat ccggtggaac
     8701 gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt gtctggaaga
     8761 agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg ttttttctcc
     8821 ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag ggaattaggt
     8881 cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta tttttcaata
     8941 cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta ttcggaattt
     9001 gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat aaggagattg
     9061 tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg gctagaagca
     9121 ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa cggacgtgca
     9181 cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg tacagcagat
     9241 cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt gtttgaaaac
     9301 gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc tgtagctgga
     9361 aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac aaaaaaagca
     9421 atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact gacggaaact
     9481 ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt aactctggat
     9541 acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg agcaccattg
     9601 aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga ccgtccattt
     9661 tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat aggccataat
     9721 atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt cagtcaagaa
     9781 atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat tgaattagat
     9841 attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa tgcagaagaa
     9901 atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt caaaacggat
     9961 aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg agaagaagaa
    10021 actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa aacatggagt
    10081 aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa agcaaccaaa
    10141 aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg gggaactcgt
    10201 ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc aattggatgt
    10261 ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga aatcaatctt
    10321 ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc tgaaaaagct
    10381 agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc aaaaaaaatc
    10441 ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga attgtcggaa
    10501 gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca tcgcgtaccg
    10561 gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca atggcaaaaa
    10621 gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa tattgaatta
    10681 acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga tgttttgctg
    10741 aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga tcagcttaaa
    10801 gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc tgaaacagac
    10861 tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa agtaatcgaa
    10921 caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc atttatgact
    10981 ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga tttcaaaatg
    11041 gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga catcgatgat
    11101 aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga atcacaatcg
    11161 ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca atatcctttc
    11221 tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt ttatcgttca
    11281 ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac ttaccctctc
    11341 catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt agatgttgct
    11401 gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg accaacaaat
    11461 gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg ttattttccc
    11521 tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca gcaagaaata
    11581 gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg gatgtcagag
    11641 catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga tgcaacacat
    11701 gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt taatgtatat
    11761 caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct atagatgttt
    11821 tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg atcgctgatt
    11881 atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat accaaaagag
    11941 ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc tttgtgacgc
    12001 ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat gagaacgagg
    12061 caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa cagatctcga
    12121 tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa gcggccgaac
    12181 tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta ggagcaatcg
    12241 gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt gatcctgaac
    12301 tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca gtgatcaatc
    12361 atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac ggatatgaag
    12421 aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt ctggaatggt
    12481 ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa aaatgatgga
    12541 ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta aaagttactt
    12601 ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt cacaaggaac
    12661 aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt cggaattggc
    12721 aggagaacac cgtttatt
//
LOCUS       DANJMI010000073        12734 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000073 DANJMI010000000
VERSION     DANJMI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.073"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(87..266)
                     /gene="rpmF"
                     /locus_tag="PWU81_002593"
     CDS             complement(87..266)
                     /gene="rpmF"
                     /locus_tag="PWU81_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL1111257.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(324..893)
                     /locus_tag="PWU81_002594"
     CDS             complement(324..893)
                     /locus_tag="PWU81_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDL1111258.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1079..1981
                     /locus_tag="PWU81_002595"
     CDS             1079..1981
                     /locus_tag="PWU81_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL1111259.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="PWU81_002596"
     CDS             complement(2016..3800)
                     /gene="pyk"
                     /locus_tag="PWU81_002596"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL1111260.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="PWU81_002597"
     CDS             complement(3874..4836)
                     /gene="pfkA"
                     /locus_tag="PWU81_002597"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL1111261.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="PWU81_002598"
     CDS             complement(5004..8318)
                     /gene="dnaE"
                     /locus_tag="PWU81_002598"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL1111262.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8549..8734
                     /locus_tag="PWU81_002599"
     CDS             8549..8734
                     /locus_tag="PWU81_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDL1111263.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8796..9992)
                     /locus_tag="PWU81_002600"
     CDS             complement(8796..9992)
                     /locus_tag="PWU81_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL1111264.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10162..11598)
                     /locus_tag="PWU81_002601"
     CDS             complement(10162..11598)
                     /locus_tag="PWU81_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL1111265.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11891..12700
                     /locus_tag="PWU81_002602"
     CDS             11891..12700
                     /locus_tag="PWU81_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1111266.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3802 a         2672 c         2250 g         4010 t
ORIGIN      
        1 atatttttta tttaccgact acaaaaaaat aaacctcaag gctccatggt tagccttgag
       61 gttttacatt ttctaattaa aatgtattaa gcttcttttg acattacgtc cttaccatca
      121 tagtaaccac aatttgcaca tacgtgatgg ctttttttca tttcaccaca gtttggacat
      181 tcgtttaacc ctttgattgt caatttgtag tgtgtacgac gttttgcttt tttagctttt
      241 gatgttcttc tagctggtac tgccattttg ctacacctcc ttgtaaagtc ttgtatctaa
      301 attagacaca atatattcca atcttacttg ttatcgtcgt cttttgattt agaatcaaaa
      361 agttcagata gttttgcaag acgaggatca attttattct cggcttcttc catttttctg
      421 cgctggtatt cttcttcgga aacaacttcc caatcattgc cttttggaag ttctgttgct
      481 gcttcctctt cttttgtcaa tacccgcatt ggaatcgcca ataagatgtt gtcttcaatc
      541 gactctgcta aattcaataa tggaccttca attagcaaaa tctcatctgc agacatttgt
      601 tcatctctac tttggaattg ttcttctgtc atgaatactt cgtctacaga aaaatccatg
      661 ggcagttcaa caggttctag tgaacgtgtt gaaggaactg tcaaagtcgt ctgcacagtg
      721 taatgaacga tatagccttt cttatcaact gagaccaaac cttcgacttc cactggcgca
      781 acatcaagta ttaaattgtc tcgcttcatg agagcggctt tcaagtcgag cgtctcatga
      841 aaagttaacg gtgtctcctg gtattttctc aattccaata aagaccattt catttttcat
      901 cactcctaag caacaaaagc aattatacag ggcaaaaagc atgttgtcaa tgaaattttc
      961 ttgacacctt gcagttcata aaaaaacaga aaaatcttta ttagaaagcc tctgtttgct
     1021 atcatcctca gaatcattta caataatcat tattccatta aaggagcgga tgatcttcat
     1081 gaacacgaaa gatatcgaat actatgtcca aatcgtcaaa caaaaaaatt ttacgaaagt
     1141 ggctaattat ttccgtgtga gccaacctac tattacatat gcattacaac gtcttgaaaa
     1201 agagtttgac accgtactta ttacaagaga tcgatcgcat cacgaattaa tcgtcacgcc
     1261 ttctggaaag cagctgtttc tccatgcaca aaccattctc caagagattt ctactgcaag
     1321 agctgaaatc actcagttgc aaaagaaaaa gcttcgtttt ggcatgcctc ctattatcgg
     1381 caatgaatac tttcctaaac tgtcaagtta ctttatcaag catgaattga tgaatcaaat
     1441 tgagattacc gatggaggct ctcgggactt atacgggatg atccgacaag ggaggatcga
     1501 tttagctgta ttaggctcaa cacagccaat cgtggatcaa tatttagaaa cagagttact
     1561 tttggacaaa cgtttcatga tcgtcgtgcc acctacacat cctctttcca agcaggatac
     1621 tgtctctttc aaagatatag cagatgaaca gtttgtacta ttgaacgaac attatgtcca
     1681 ccccgcagca ttcactaagc tttctaaaca aacacatact catccaaaag ttgtttatca
     1741 aagcaatgac ttgacgattt taaagagtat ggtccgagaa aatgtgggta tcggtttttt
     1801 ggcagagatt gctgtccatc ctgaagatca tttagtttgc ctccctcttt cagatgaagt
     1861 tcagcctcgc ttcttgattt cattggcaaa acgaacacag cagatcgtca cgcctcttca
     1921 agaacaagta gtccaagtta tccatgaatt cgttacagca caaaaaaaca gctcttcgta
     1981 aaagaagagc tgtttttatt ttttattaag ctaaattaga tagctgttgt ttcgccacgg
     2041 taaacgatgc cgcgacgtga atcaacggta atcaattcac cttctgtaat tgttgttgtt
     2101 gcgcctttag caccaacgat tacaggaata tcttgtgcaa ttcctacaac tgcagcgtga
     2161 gaagtcaatc cgccttcttc aacgataaca gcagctgctt tttcgattgc aggcatatat
     2221 tctttgtcag ttgatggtac aactagaacc attccgtctt ttgctttttt tacagcttct
     2281 tcagcagatg aagcaataac tgcatttgca acgacagatt tttgtccaac gccttgagct
     2341 tctaataatt tagaaccaat catttgaatt ttcatcacgt tagttgttcc gcgttcacca
     2401 actggtacac cagcagtgat caagattaaa tcgccttctg atgcgaaacc taattcaaca
     2461 gcttttttag ctgctagttc aaacatttca tcagttgttg atggtttttc agcaactgtt
     2521 gggtaaacgc cccagtttaa catcaaacca cgttttgtgc gttcgtcaaa agtaacagcc
     2581 aagatatctg catctggacg atattttgaa atcatttttg cagtgtatcc tgattcagtt
     2641 gcagcaacga ttgttttcac gcctagattt tttgcagcac gagctacaga taaaccgatt
     2701 gtttcagtta catctgtttt atcgaattcg ttcaattgga atgtacctaa ttctgataat
     2761 gcattttcag cttcttggtc gatacgtgcc attgttgaaa cagcttcaac tgggtagtcc
     2821 ccgtttgcag attcaccaga tagcattgtt gcatctgtgc catcaaatac agcgttagca
     2881 acgtcagaag cttccgcacg tgttggacgt gggttttctt gcattgattc taacatttgt
     2941 gtagcagtga taactgggat accagcagca ttacattttt tgataatgcg tttttgaacc
     3001 attggtacta gttcagctgg gatttcaaca cccatatctc cgcgggcaat cataacaccg
     3061 tcagaaactt tgatgatttc gtcaatgttg tcgatacctt cttgagattc gattttaggg
     3121 aagatttgaa catgagtcat gtctttttct tctaagattt cgcggatatc tagaacgtct
     3181 tgtgctttac gtacaaaact tgcagcgata aagtcgatat cgttgtctaa accaaaacga
     3241 atatcgtcag catcttttgg agtaatacct ggcaaactaa tagaaacgcc tggtgcattt
     3301 acccctttac gtgatcctag catacctgca tttttaactt ctacgactaa ttcacggttt
     3361 gcttcgtctt tttcaatgat ttgcatatcg atcaaaccat catcaaataa aatgtgtccg
     3421 ccaacgtgaa catcgtcaaa taatcctggg taagtaaccg cgattttttc ttttgaacct
     3481 acatgttcag gatccataga gatgcgtgtt tgatctccta cttcaaattt gatgtatcct
     3541 gcacgaccaa aatcagcttc tgttgtgcct tgaacagttg tacggatttc agctcctttt
     3601 gtatctaaaa gaataccgac atcttttcct gtctttttaa ctgcttcacg gaccatagtc
     3661 atacgtgata attgttcttc atggtcacca tgagaaaaat taaagcggaa gacatttgcc
     3721 ccagattcaa tcaattgaga aataatttct actgagttac ttgctggtcc taatgtacta
     3781 acgattttgg tttttttcat ttaaaaaact ctcccattca attttgttta tttaaagctg
     3841 tacacagctt aaatagcttg gtttctttca ctcttaaaat gaaagtgaat tatttagatc
     3901 atatagtgat aagtctggtt tatgtttgtt gttttcaagt gtatcaatga tgtcagacgc
     3961 aacgatttcg ttgtcatgga taccgacaca taatccacct tttccttctt gaagcaattc
     4021 gacagcgtaa gcaccgaatt tacttgccaa cacgcggtcg cgtgcgcttg gtgctccacc
     4081 acggacaacg tgtcccaaga tagatacacg tgtatgatag tcaccaaatt ctgaaagttt
     4141 ctcagcgaat tcatttccgc ccattacacc ttcagccaag atgatcaagc aatgtttctt
     4201 cccgcgatcg cggccttctt ggattttttt agcaactgaa gccatatcaa aatcatgttc
     4261 tgggatgatg atttcatccg ctccgccagc cacacctgac cataaagcga tgtctcctgc
     4321 attgcgtccc attacttcga taacgaatgt acgaacatga gaagtcgctg tgtcacgaat
     4381 acggtcaatt gattctagta ctgtattgat tgctgtatca aaaccgattg tgaaatcagt
     4441 cccaggaata tcattatcga ttgttccagg aatacctact gctgggaaac cgcgttttgt
     4501 caaagccatt gcgccatgat aagagccgtc tcctccgata accactagac cttcgattcc
     4561 gaattttttc aattgttcga ttccttttaa ttgaccttct tcagttgcaa attctggata
     4621 acgtgcagaa tacaagaatg taccaccacg ttggatcttg tcacctacat ctgccacatc
     4681 aagacgacga atatctccag caactaaacc ggcaaaacca taattgatcc cgtatacttc
     4741 cagtccttcg taaatacctt tacgaacaac ggcacgaaca gctgcattca ttccaggtgc
     4801 gtctccgcca ctggtcaaaa ttccgatgcg tttcataata aacattcacc tcattaaaat
     4861 actttatcct aagtatgact taggtttaca accatttttg cgttaaccac gactaacatt
     4921 ctaacattat ttttctcact tgtcttgatt tacggataaa aaaatgaaaa aaaatgaaag
     4981 caagtagtat tttttaccgt aagttatttg aaaaccacat tatcttcgcc aagcagataa
     5041 ataagttgcc cctttatact cttgctgtca tccacccaat aagcatctga cagaactact
     5101 tttcgttgcg ttttttcgtg atacataact actggtatat atcctgggtg tttctgaaaa
     5161 atcacttgta attgtttcat tatctcggga tcttcccgtt cttctgtaat acgcaaatag
     5221 caagtttgtg aaggatactg ggcttctaac tggttaggat cagcaaagct ttctccaatt
     5281 agttgcagtt cctgattata tttgctgatt tcgctccgcc cttgaacaaa gatcactcga
     5341 tcaacggcaa acaaattacg atatttacga taaagagttg gaaaaactgt gacagaaatt
     5401 tctccactac tgtcggttcc ttcaacaaag gccatcagtt ctcctttttt tgtccgaatt
     5461 tctctaactt ccttgatcat cactaataaa ttgactggtt gattagggac gatatctgta
     5521 atcagctgaa cttgtttagc taaacgaaga cgatcaaacc cttctgcagg atgacctgac
     5581 aaataaatac ctaagtactc ctcttccagc ttcaagcgtt cttctagtgt atagtcagcg
     5641 acttcttctt cttttaatgc catgattccc agcaagtcaa gacttccacc actgtaaacg
     5701 atattttgga tttttccttc gagatcaagc atcaattgtt tcctgttagg agataattca
     5761 tcaaaaactc ctactgctgt cagtgattgc agtaactcta atttcagcca acgcttatcg
     5821 atccgtatga ggaattgatc cactgactgg aacgggccat tttgttttct ttcgtttaat
     5881 atatcttcta caaaatcacg ccgaatacct tttatcgctg tcagtccgaa tctgatttct
     5941 tccttattga ccaactcaaa actggaatta ctttgattaa tggacggcga ggaaagtttg
     6001 agtttatttt tacgtgcttc tgcaatatat tctttcaatt ttgatgtgtt atgccgaaca
     6061 gaattcatca ggctggtaaa gaaagcacct ggatgatggg cttttagata cgccatttgg
     6121 aatcccacga atgaataagc aaatgcatgt gaccgattga atccatagtt tgcaaatttt
     6181 tcgatatatt catatacggt cgctgctgtt ttttccgaat atccctgttg taatgctcct
     6241 gatacaaaat gcgacttttc ttcatctaat acgtcttttt tctttttact cactgcccgt
     6301 cgcagaatat ctgactgccc taatgtaaat ccagccatag tcgaggcgat ctgcatgatt
     6361 tgttcctgat agacgatcac tccatatgtg tttttcaaaa tcgggatcag tgaatcatct
     6421 gggtagcgaa tggcttcttt tcctttcttc ctggcaatga aggtatctat attttgcata
     6481 gggcctggtc gatacaatgc attgactgcg gcaatatctt cgatgttttc tggatctaat
     6541 cttcgtaaaa cgttccggat accagcagat tcaaattgaa atactcctga ggtttcgcct
     6601 cgttgaaaca agagcagcgt ttttgcatca tttagcggga tctcttttaa tcggatctca
     6661 tacccttcta ctcgcttaac agctttcaaa gtaaaatcaa taatcgataa gttgcgtagt
     6721 cctaaaaagt ccattttcaa aagaccgatt gcttctacct cattcatcgt atactgggtc
     6781 agaaaagctt catcagaacc gcgttggagc ggaactagtt caagtagatt cttatcgctg
     6841 ataactacac ctgctgcgtg agtcgaaaca tgtcttggca atccttctaa aatcactgca
     6901 gttttgaata aaagttggtt tttttcgctt gcctgaacta attctactaa ttgatgcgaa
     6961 gttttataag cttgggtcaa tgtgattttc aattgattgg gtattgcttt tgaccagcgg
     7021 tttgcttcac tttgcgataa accaaacact ctagctgcat ctctaagaac catttttgct
     7081 gccattgtgc caaatgttgc gatttgtgcc atatgatatt gtccatactt ttctcgaaca
     7141 taattcaata cttcttcacg tcgattgtca ggaatatcca aatcgatatc tggcatcgta
     7201 aagcgttcgg ggtttaaaaa gcgttcgaac agcaaatcat attctaatgg atccacatct
     7261 gtgatttcta acacataggc tactaatgaa cccgcagcag aaccacgacc tgcaccggta
     7321 acgattttgt ttctatgagc aaaagccatt acatcccata cgatcaagaa ataatcatcg
     7381 aatcccattt gatggatgac agataactcg tagtccaaac gttcttggta ttcctttgta
     7441 tagtcagtga ttcggcgttc cacttgcgaa tagcataact ctcttaaata ttctccagct
     7501 tgttttccat taggaactgg ataatgcgga agtaatttct gatggaactg gaattcaaat
     7561 gaaatcgatt cagctaaacg ctgggcgcct attacagctt gggaaagacc cgctttttcg
     7621 aatcgtttga tcatttcttg ttctgaaaga agatagacat ttgctgttag tggtaaagat
     7681 gcagataaat tttctaaagg tatttggctt ccctctttga tatgtttcat cacttcaagt
     7741 gcaaaaagtt cttcttgttg taaactagcg atgggatgta atgcacaaag cgaataattt
     7801 tcttttatca catactgata ccattcaggc aattgatcag gcgtgttttc tgtcaagccg
     7861 atataaaaat ttctagtaaa aacattttca agtaaaaact gccaatgacc gaaaaagtta
     7921 tcctctttat tgaaagcctc agaaacagaa tgatttgccg gaacaacaaa cgctagatgg
     7981 tcggtttctg tcatttctgc aagttccaat ttttcttgga tcatttttcg gctggaaagt
     8041 ttcatcagct gctgataacc tatttcatct tttgcgtacg caagcaagcc ataatttttt
     8101 tcttcataag tataactaag atgtaatcca atgattggct gtactccttc ttctacacaa
     8161 atacgataga attcaggtgc accgtataag acatctttat ccgttatggc taatgaagtg
     8221 tagccttgtt tttttgcttc ttgtacatat tctcgaatac gtatcgtact ttgtaaaaga
     8281 gaataagaag taattgtata aagttgtggg aaaaacatct tgcattccgc cttttttttc
     8341 gttaaaattc actagcgtta aaaggtttga tccttttact ttcattaaga tagcaggcgc
     8401 tgaagttgat tattccactt catttgcttg cttgtaatct gtaggaatga gttctttcgc
     8461 aagaactcaa attttattga gatttggtct cgtttcttta cattcaaaga aaatctggta
     8521 aaattaagat agaaaagagg tgttctttat gcgatatatc gttacattat tctgggctgt
     8581 cgttctaggt caagtagtcg gttatattgg tgctgcatta acaagcggaa catatgattt
     8641 tactttgaca acaatcattt cattcattgc gggtgtcatt atccttttga ttggtgcagt
     8701 tgcgccaaga aaagaaacct ctgcccattc gtaaaaaaac agccaagagc ttgagatttc
     8761 tcaagctctt ttttattttc tactcctcac caccattata atctgctgcg aagaacttta
     8821 cgaaagttga ataaagaaag aagaatattt gacagaacaa acaaacttgt cacaataaaa
     8881 acagcactgt aattataagc atgcgcgact gtcgatccta gcatgggacc taaaacttgt
     8941 ccaaagttgc taaacatctg attgtagcta tagattcggc tgactccttc ttgaggcgta
     9001 atcttactga tcagtgtatt gacagaaggc atcaaagcgc cagttgaaaa gcctaaaata
     9061 aagcgaagta tacctaattg gagcggtgta gtgacgaatg ccataggtaa ataacagcaa
     9121 aatgataaaa tgagtcctcc aattagaact ttttgattcc caatcttatc ccctagtttt
     9181 cctagtttcg gtgaagaaaa gacagcggaa acaccagcta tcgaaacaat caagccgctg
     9241 acaaaaagga tattttctgt acttccgctc aattcacgaa tatataaggt caaaataggg
     9301 ctgatcgtag tgattcctat ctgtaaaatc aaggtcgtaa tgaagagacc gatcaaaata
     9361 gagagatgat ccatttttga aaaaatttct ttggtgctta tcagatcttt tttctcgatt
     9421 ggatggaaat cttctttgac taagaaaatg gtcaataccg ttgtgatcat caacaaagcc
     9481 cctgtgatca agaatacatt ttccatacca aaccactgag ccagtgcacc gcccattgaa
     9541 ggtccgatca gatttccagc aatcgctccg gtagacaatg ttcctaacgc ccatccgctc
     9601 ttttctctag gtgcctgtga agcaatcatc gctgtggcat ttggaatata tccagacaaa
     9661 atcccattcc aaaaccgcat gatcaaaagc caatacgcgt ttggtacaaa agccaaagat
     9721 cccattgtta atgtcatccc tgctgctgca cggatcatca tgagttttcg gccttttcga
     9781 tcagctagat ttccccagat tggtgcaacc acggcagatg caaaagcagt cacagagatt
     9841 gctagtcccg aaaacaattc gatttgatct tttggtgttc ctagttgctc aacataaact
     9901 ggtatgaatg gcataactaa actaatactt gcaccagtga aaaaacagcc aatccacgca
     9961 atcactaaat tttttctcca attaatctcc atctatcatc cctctttcgt ctcttttatc
    10021 atacctattt tcgaataaac aagcaacgat ctcgccagta aaaagagaaa aatcccgcaa
    10081 aggtcatttt atatgtaacg ttctttttat gtaaaacaaa aagagatcag aacaaatact
    10141 gttccaatcc cttctcttta gttaaattgt tgctagaatg atgaagttga taatgaataa
    10201 aatatcaacg atccacaaag caacagaaac ttgattgtat tttcctttga ttactttcac
    10261 aattgtatag aaaataaatc ctgctgcaat cccataggaa atgctatagc acaatcccat
    10321 aaagatcgat gcaaagaagg ccggtaaagc ttcttccata tctagccatt tgatgtctgc
    10381 aaaagaagcc atcatcatta ctccaactaa aatcaatgct ggcgcagttg cttgatttgg
    10441 tacaatggcg atcagtggag agaataaact acttaatgcg aatagcactg ccactactac
    10501 tgaagtcaaa ccagtacgtc caccggcccc gatcccagct gcagattcga cataagttgt
    10561 tgtattcgat gtaccaaaga tagcccccac agaagtagcg atcgcatcgg caaatagtgc
    10621 tttatccatt tttgttttga acccacgacc atcttccaac gcgatctcgt cttcttttga
    10681 gaaaatacct gtgcggcgtc ctgtaccgat aaatgttccg atcgtatcga aagtatcaga
    10741 taaactgaat gcgatgatcg tcatcaatac ttgaggaatc ttggaagaat cactgaataa
    10801 agattgcatg ccttcagaac caaatgctgc accgaaagtt gttcctaatt ctttaaatga
    10861 attacctaac gaattcgttt gccaatcaat ggaacttaaa tcaacaacac ccataaaaat
    10921 accaaggact gtggtcgcta cgataccgat aagaatagcc ccacggacat tttttacaac
    10981 taaaatggct gtcagtacca aaccaatcac agccaataag ataggagcgt tgtcaaagtt
    11041 ggctaacgca gggacgattc caccatttat cgtaacattt gtcgcttttc cgccttcaac
    11101 gacagagctc gaaatagctg actggtctgc tgaaaaagat aagaatcctg catttttgat
    11161 tccgacatac gcaacgaaaa taccgatccc gccaccaatc gcatgttgaa gactttcggg
    11221 aatcgcccga ataatcattt ttcgaatatt tgtgacagta ataaaaatat tgattaatcc
    11281 gcaaataaac accattgcca aggcttgttg ccaagaataa ccaagcccaa aaacgaccgt
    11341 aaaagtgaaa aatgcgttca atcccatacc aggtgcctga gcatatggta catttgcgaa
    11401 caatcccatt accagcgtac caataatcga tgcaatgatt gtcgctagaa atactgcttg
    11461 gaagggcata cctgatgctg acaaaatcgt cggatttacg aataagatat aactcatggc
    11521 aaagaatgtc gttactcccg ccattatttc agttgataca tttgtattgt ttttctttaa
    11581 accgaaaaac ttttccataa aaagttccac tccctgaatt tttttctgat ggaaaactat
    11641 aataataaaa aataagtaaa atagggcaaa tccatcgaac aatttcaaat tatatagacg
    11701 tttcattcgc ttttcaacgt ttttttaaca aaaaaagcga aaataatact taacgttcgt
    11761 gtttagctta tgtttagcta tttatatagc cataactgta gtttttgtta ggaatagctg
    11821 attgctcttg ttaattacct aaaatatgct agactaaaac aaatgctagt tgaggaagga
    11881 aggaccctta ttgaataaaa aattaattgc catcgatctg gacggaacta ccctcaatca
    11941 agattcgatg attacatcta aaacaaaaga gactctaaaa aaagcaatca atgccggtca
    12001 tcatgtcagt attgctacag gacgcccatt ccgaatgagc catcagtttt accaacaatt
    12061 agaattaaca acaccgatgg ttaattttaa cggcgcatta gtacacatcc ctaatcagca
    12121 ctgggatggt gaaagagaaa cgttgatcaa tcgtgaaatc gcttttgaaa tcttatcgca
    12181 aaaaaagcag ctaaatttag attttatcgc tgctgaaaat cgtgatactt tttttattga
    12241 cagttttgat ttctttgatg aaaaaatttt tgcttcttct cgtccaggag agaagaattt
    12301 gctttctcct aaaaatttga cgacaaatcc tacatctttg cttgtccgaa cagacaaacg
    12361 ttttgcagga gctgtatctg ctgaattaac acgtcaattt ggcagttatg tcgatgttcg
    12421 gacatggggt ggacctactg ccatcttaga gattgtttca aaaggaatcc aaaaagcaaa
    12481 aggcgtgcaa gagattgcta actacctgtc tatcgatcaa aaagacgtca ttgcctttgg
    12541 tgacgagcat aatgatttgg aattgttgga ttatgcaggt tggggagtag ctatggctaa
    12601 cggtactgac caactcaaag gcattgcaaa tgatgtcact cctttgagca atcaggaaga
    12661 cggtttagct gtttatctgg aaaaactact taaactataa tagagaatta gattccacaa
    12721 aaaagaggtt gtga
//
LOCUS       DANJMI010000074        12556 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000074 DANJMI010000000
VERSION     DANJMI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.074"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..776
                     /locus_tag="PWU81_002603"
     CDS             <1..776
                     /locus_tag="PWU81_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL1111267.1"
                     /translation="DSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYL
                     VKITEEKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANAD
                     VEKELYANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMT
                     RRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFF
                     KVSLDEIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            848..1969
                     /locus_tag="PWU81_002604"
     CDS             848..1969
                     /locus_tag="PWU81_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1111268.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(2051..3823)
                     /locus_tag="PWU81_002605"
     CDS             complement(2051..3823)
                     /locus_tag="PWU81_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1111269.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(3826..5538)
                     /locus_tag="PWU81_002606"
     CDS             complement(3826..5538)
                     /locus_tag="PWU81_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1111270.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            5653..6321
                     /locus_tag="PWU81_002607"
     CDS             5653..6321
                     /locus_tag="PWU81_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDL1111271.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            complement(6388..7176)
                     /locus_tag="PWU81_002608"
     CDS             complement(6388..7176)
                     /locus_tag="PWU81_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL1111272.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(7259..8731)
                     /locus_tag="PWU81_002609"
     CDS             complement(7259..8731)
                     /locus_tag="PWU81_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1111273.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            complement(8861..10054)
                     /locus_tag="PWU81_002610"
     CDS             complement(8861..10054)
                     /locus_tag="PWU81_002610"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL1111274.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     regulatory      complement(10052..10131)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            complement(10320..10391)
                     /locus_tag="PWU81_002611"
     tRNA            complement(10320..10391)
                     /locus_tag="PWU81_002611"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10356..10358),aa:Glu,seq:ttc)
     gene            complement(10403..10476)
                     /locus_tag="PWU81_002612"
     tRNA            complement(10403..10476)
                     /locus_tag="PWU81_002612"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10440..10442),aa:Ile,seq:gat)
     gene            complement(10489..10559)
                     /locus_tag="PWU81_002613"
     tRNA            complement(10489..10559)
                     /locus_tag="PWU81_002613"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10525..10527),aa:Gly,seq:tcc)
     gene            complement(10564..10636)
                     /locus_tag="PWU81_002614"
     tRNA            complement(10564..10636)
                     /locus_tag="PWU81_002614"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10601..10603),aa:Phe,seq:gaa)
     gene            complement(10640..10712)
                     /locus_tag="PWU81_002615"
     tRNA            complement(10640..10712)
                     /locus_tag="PWU81_002615"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10677..10679),aa:Asp,seq:gtc)
     gene            complement(10731..10804)
                     /locus_tag="PWU81_002616"
     tRNA            complement(10731..10804)
                     /locus_tag="PWU81_002616"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10768..10770),aa:Met,seq:cat)
     gene            complement(10817..10906)
                     /locus_tag="PWU81_002617"
     tRNA            complement(10817..10906)
                     /locus_tag="PWU81_002617"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10868..10870),aa:Ser,seq:tga)
     gene            complement(10929..11000)
                     /locus_tag="PWU81_002618"
     tRNA            complement(10929..11000)
                     /locus_tag="PWU81_002618"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10965..10967),aa:Glu,seq:ttc)
     gene            complement(11022..11109)
                     /locus_tag="PWU81_002619"
     tRNA            complement(11022..11109)
                     /locus_tag="PWU81_002619"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11073..11075),aa:Ser,seq:gct)
     gene            complement(11134..11207)
                     /locus_tag="PWU81_002620"
     tRNA            complement(11134..11207)
                     /locus_tag="PWU81_002620"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11171..11173),aa:Ile,seq:gat)
     gene            complement(11221..11291)
                     /locus_tag="PWU81_002621"
     tRNA            complement(11221..11291)
                     /locus_tag="PWU81_002621"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11257..11259),aa:Gly,seq:tcc)
     gene            complement(11293..11368)
                     /locus_tag="PWU81_002622"
     tRNA            complement(11293..11368)
                     /locus_tag="PWU81_002622"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11333..11335),aa:Asp,seq:gtc)
     gene            complement(11387..11460)
                     /locus_tag="PWU81_002623"
     tRNA            complement(11387..11460)
                     /locus_tag="PWU81_002623"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11424..11426),aa:Met,seq:cat)
     gene            complement(11473..11562)
                     /locus_tag="PWU81_002624"
     tRNA            complement(11473..11562)
                     /locus_tag="PWU81_002624"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11524..11526),aa:Ser,seq:tga)
     gene            complement(11586..11659)
                     /locus_tag="PWU81_002625"
     tRNA            complement(11586..11659)
                     /locus_tag="PWU81_002625"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11623..11625),aa:Ile,seq:cat)
     gene            complement(11673..11746)
                     /locus_tag="PWU81_002626"
     tRNA            complement(11673..11746)
                     /locus_tag="PWU81_002626"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11710..11712),aa:Met,seq:cat)
     gene            complement(11774..11846)
                     /locus_tag="PWU81_002627"
     tRNA            complement(11774..11846)
                     /locus_tag="PWU81_002627"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11811..11813),aa:Ala,seq:tgc)
     gene            complement(11866..11939)
                     /locus_tag="PWU81_002628"
     tRNA            complement(11866..11939)
                     /locus_tag="PWU81_002628"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11903..11905),aa:Pro,seq:tgg)
     gene            complement(11949..12022)
                     /locus_tag="PWU81_002629"
     tRNA            complement(11949..12022)
                     /locus_tag="PWU81_002629"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11986..11988),aa:Arg,seq:acg)
     gene            complement(12033..12118)
                     /locus_tag="PWU81_002630"
     tRNA            complement(12033..12118)
                     /locus_tag="PWU81_002630"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12082..12084),aa:Leu,seq:taa)
     gene            complement(12135..12206)
                     /locus_tag="PWU81_002631"
     tRNA            complement(12135..12206)
                     /locus_tag="PWU81_002631"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12172..12174),aa:Gly,seq:gcc)
     gene            complement(12218..12290)
                     /locus_tag="PWU81_002632"
     tRNA            complement(12218..12290)
                     /locus_tag="PWU81_002632"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12255..12257),aa:Thr,seq:tgt)
     gene            complement(12308..12389)
                     /locus_tag="PWU81_002633"
     tRNA            complement(12308..12389)
                     /locus_tag="PWU81_002633"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12353..12355),aa:Leu,seq:tag)
     gene            complement(12397..12469)
                     /locus_tag="PWU81_002634"
     tRNA            complement(12397..12469)
                     /locus_tag="PWU81_002634"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12434..12436),aa:Lys,seq:ttt)
     gene            complement(12473..12545)
                     /locus_tag="PWU81_002635"
     tRNA            complement(12473..12545)
                     /locus_tag="PWU81_002635"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12510..12512),aa:Val,seq:tac)
BASE COUNT         4022 a         2698 c         2225 g         3611 t
ORIGIN      
        1 tagactcgac tagggaaaaa atcataaaaa tatttgataa aattaataat ttattcaaga
       61 atgacaatgc tgaattaagg aagggtcttt tagagtttaa acttcaagaa ctagaacttc
      121 attactctta tttagtgaaa attaccgaag aaaaagaaca acaaaaagca ataaaagaac
      181 agatgataga agaggaaaaa gttcgtagag aaattgatcg tgaaaagaaa cgtattgata
      241 aagaacaacg tcaattcaat tctgaaattt ccaaattaat cgcatatatg caaaaagcta
      301 atgcagatgt ggaaaaagaa ttatatgcaa ataaaattca agaactggaa gaaaaattaa
      361 aagaattaga agttatacgt gaaaatgtat tacaaagaga attaaatact agagccggtt
      421 atgtgtatgt aattagtaat attgggtctt ttggtgaaga catatataaa attggtatga
      481 ctagaagatt agaacctatg gatagaataa aagaattgag tagtgcatcg gttccatttg
      541 aatttgacgt tcatgcgatg attttttcag aggacgctcc gtctttggaa actaaattgc
      601 ataatcattt tagaaaacaa gaagttaata aaattaatca aagaaaagaa tttttcaaag
      661 tttcactaga cgaaatagag aaagttgttt tagaaaatta taatggaaca gtaacattta
      721 caaaattagc taaagctgaa caatatagac gatctttaga attgtcaaaa gattaaatat
      781 ataaaaagcc ttcgggcttt ttcttttaac aattaatagg aacgtatatt cgaaaggaat
      841 agaaataatg tggttagaaa aaataaatga caaaaaattt aagtacaccg aacgttatac
      901 ggatccctta actgaaaaaa agagaaaagt gtccgttact ttatctagca attcacgcca
      961 agcttggaat caagctaact tacttttgaa tgaaaaaatc gcagaaaaaa taaaacgaaa
     1021 tgaagaatta ccactaactt ttggagaatt aaaaactaaa tgggatgaaa aatacaagcc
     1081 aactgtaaaa gaaagttcct atcgaacaac tcaggtttat ctatcattaa tttcaaaata
     1141 tataaaagat gatgtacttg taaaaaacgt taatagtaat cttatacaag atatgctaga
     1201 ttactactat tttgaaaaaa atttatcata taattatgta atacatttaa aaacttttac
     1261 aggaatgatt tttaaattcg ccaaccgtcg atatggatta aaatataatc ccgtaaatgg
     1321 ggtttcttta gcaagaaaac ctaagacaaa cgaagaaata aaaaaagaaa aagatggtta
     1381 tttatcaaaa gaagaagttc aaatgattgc tagtaaacaa aaaagttcac atcaacagtc
     1441 acgttattct ttaattacta aatttttgtt tttaactgga ctacgttatg gtgaattgat
     1501 atctttgact gaatctgatt atgatggtaa taaaattacg gtttctggta cttatgatta
     1561 cgagttaaaa attaaaacaa caactaaaaa tacaggttct tatagaacca ttgaattatc
     1621 aagtaatgct aaagaaatca tagatcaatt aattgaagaa aacaaattaa ttaagaataa
     1681 taaagataaa tacattttca tctctaaaaa tggtaacccg atatctattc aagcatataa
     1741 tcaatcttta agagcagtat ccaaagaatt aaacctagat aaaaaagttt cttctcacat
     1801 gttaaggcat tcccacattt ctctacttac agaattagga atcccattaa aagccattat
     1861 ggatcgtgta ggacatgaag attcaaaaac aacattaaag atttatactc atacaactaa
     1921 aaatatgcaa aatcaactag tagaaaaact aggaaaaata gaaatttaaa ttgccccttt
     1981 tctgcccctt ataaaaacaa acaacataaa atatataaaa agaaccccgt tataacgagg
     2041 ttcttttaat ttaactttca aatacaaatt gattatgata aagttccgca taaaatccat
     2101 tttctgctaa taactcgtga tgactgcctt cttcaatgac ttccccatca cgcaagacaa
     2161 cgattctatc tgcattcaag attgttttca gccggtgagc aatcacaaag ctggttcttc
     2221 ctttgattgc ttcatccatt gctttttgga tccttgcttc tgttaccgtg tctacattac
     2281 ttgttgcttc atccaaaatc aataaagatg gatttgtgat gatcgttcga gcaatactga
     2341 tcaactgctt ttgacctgta ctgaataagt tattttcttc ggatatctct gtatcataac
     2401 ctttttctaa cgtcatgatg aagtcatgaa tattcgcttg ttttgctgca ttgatgactt
     2461 cttcatctga agcgtccggt ttgccgaatg tgatattgtc tttgatcgtt ccggagaaca
     2521 atacagattc ttgcaaaacg attcctacat gtctacgtaa tgcatcaaga tcataatctc
     2581 ggatatctat tccatcgatg actactgaac catcattgac atcgtaaaaa cggtttagca
     2641 gattcatgat cgtcgttttc cccgatcctg ttggaccaac aagagcaacc atttctcctt
     2701 tgtccacatt gatcgagaca tctttcaaga ctagtttttc tggagaataa ccaaaatcta
     2761 cgtgctgcaa cgcaagacct ttttcaacac catcaaatgc tttacagttt tctggtttga
     2821 cttcatcttt ttcatcaaat atttcattca atcgtcttgc ccctgtaatc gctaattgga
     2881 tcatgctgta tccagatgaa atctgcatca gtggttggta atattgttgt gaatactgga
     2941 cgaatgtcac aactaagcct aatgccaccg aacgttccaa atcaccattt aatgctaacc
     3001 agccgccgaa aaagataact atcgctgtat tgaccagtga cattccttgc atcatcggaa
     3061 atagtagacc agaataagct tgtcctttga aggttgcttc gcgaacactt tgattatgct
     3121 ctaagaatcc ttcaatcgtt tcttcttgta agccatttgt gatgatgaca cgttgtccgc
     3181 tgattttttc atccatataa ccatttagtt tccccacttc atcttgctga agatccacat
     3241 atttccgtgc tttgcggata atcaagacag ctatcaaaac ggctactgga gtggaagcaa
     3301 ttgtagccca agctaactct acattttggc ggaacatcat gatcaagatc ccgacaagta
     3361 aaacagcatt cgtgatgact tgaagcaaag cttggttcaa actattttga atattatcta
     3421 agtcactggt aaaacgactt aaaatttcac cgtcttgatg agaatcaaag aaacggatcg
     3481 tcaatttttc caatttgttg aatagaccga tacgcattcg gttcgttgac ttccctacta
     3541 cttgtgtgaa caaaatacta tagataaagt tggctgcact agctagtaca taaaacagca
     3601 agagtttcca tattacagaa acaaatttgc ttttatcgtc tacgccttgc atcaagcctc
     3661 ctacgtaatt agctaattct tgaaaagctt ccccgatgta ttgaggtgcc tttacttgta
     3721 gataggtagc aatgatcact gtgataaaga taaagaaaaa ggagagtttg taccgcttca
     3781 aatagtgata gaaaaattta cttgctttta ttagatcagt catacttact cctccgttcc
     3841 tttttgagtt tcaaagattt cctgatagac acggtttcca gctaccaatt cctcatgggt
     3901 tccttcgccg actaatctgc cttcatccaa tactaagatg cggtctgctt tgacaacaga
     3961 agcaattttt tgtgcgatga caatcgtcgt tgttcccttc aaatctcgat cgagtgcttc
     4021 tcggaccagc ttttcagagc gagcatccag tgcactcgta ctgtcatcta ggatcaagat
     4081 ctttggatta ccgataacgc cacgagtaat ggataaacgt tgcttttgac caccagaaaa
     4141 gttactgcta cgttcttcta ttggtgcttc gtaacgatca gataactttt cgatgaattc
     4201 ttttgcttga gcaatagaag aagcatgttc catctctttt tctgttgcat tctttttccc
     4261 ttggcgcaag ttttgagcaa tcgttcctga gaataaaatt gctttttgca aaacaaagga
     4321 aaccgtttgt cttaaactct tttcatttac ttgacgaaga tctacaccac ctacttcaac
     4381 tttgccttct gtcggatcaa atagacgagg gatcaattgt gctagtgttg acttcccggc
     4441 accagtcgcc ccgacgatac caaccatttc tccaggtttg atagaaaagc tgatatcttt
     4501 aagcgtatct gtatcctcac caggataacg aaaagaaaca tggtcaaaag tgacagaacc
     4561 cgtcaactct tgttctggaa cgtctaaata agtaagatct ggttcagcat ctaaaatttc
     4621 cgcaatccgc ttcagcgata cggctgctct tgaagtcatc atcatcatca ttccgccaat
     4681 aatgattgcc atcataatct gcatcaagta gttcatgaat gaagcaattc caccaattaa
     4741 cgtaggatca tctttaacaa gatcgctcac aaaaaagatc gccccaacca cagccaaatt
     4801 ggcagccagc atgaaggacg ggatcatcac agaaaataat gtaccgacta tgatgttgtg
     4861 ttttgttaag tcctcactta ctttgctgaa tgacttcaat tggttttctt cttgtacaaa
     4921 agatttcact acacgaatcc ctagtaagtt ttcttttgca atgctattca cacgatcaat
     4981 cagtttttga atgatcatga agtgcttgcc catccttgta aatgaaagtg ccgtaatcag
     5041 aaataccgta acgataagcg ctacgatgat ccaccataat tgcggcatcg tgatcatagc
     5101 taaaatgaag gcaccaataa ataagaatgg aattcgaaaa agtgattgca aagaaatcat
     5161 cactaaattt tggatttgcg tgatatcatt tgttagacga acaacaagat ttccagcaga
     5221 gaactgttcg atatttccaa atgaaaatgt ctggatcttt cgaaatgctt cttctcggat
     5281 atctgcactt acgccttgag ctacttttgc agagaaaatc gtgttcgtta cacctgcggc
     5341 taatcctagc aaagccagac tgatcaggta aataccaatt gttttcattt catcattatc
     5401 ttctgtaata atagcttcca atacttgctg tagtaactta ggttgccata atgctgctgc
     5461 aaccatgact gctacggaca gcaaggaaat aaaaacagct actttatatt ttttcgcatg
     5521 tcgtaagatt aatttcattc gttgattaca cccctttgtt taaattgaca ggactaattt
     5581 aacactgtaa aattagaccg actaacaatt taccatgttt aaaaagataa tgcaatacac
     5641 gaggttgata aaatggaaat aaagctttct caagcaatca tcattcaggc cgcctttgat
     5701 atcctggctg aaacacaagc tctttcgcag ctttccatgc gaaacatcgc aaacagaatt
     5761 catgttcaag cacctgcttt atattggtat ttcaaaaaca aacaacagct cttgcaaata
     5821 atggctgaaa caatggaaga agaattagcg cttccagatc caactttgcc ttggaatgaa
     5881 cagttgctgc agtatatgga aaattactat gacctttata cacgtttccc ttgtggggca
     5941 gaattagaaa tcaatactgt gcccgctttt ccgagtcgat tagaacattt ggagcaaatg
     6001 atccaattac ttgttcgtga aggattttca gtcacccaaa gtcatcaggc cattgcctct
     6061 ctccacaatt tattgattgg acagttaatg gatcagcagc atgaacagca gcttcgacaa
     6121 aaaattatgg acggaaatga atttttgaaa gaaacagttc tatttatgcg taattatgta
     6181 gttgaaaatc aattagaggg atttaaagaa agtatgcagt cccatgcaga attgaatgaa
     6241 aaagcactct ttctaacgag tgcacaaacc tatattgaag gcttatgcca acaaaataaa
     6301 agaagagaaa acgagcagta aaaaagctcc tatagagatc actatgtttg tgatctctat
     6361 aggagctatc tttttaagac tcttttttta tttttcttcc attagccacg ccaatgcacg
     6421 ttgaatccat tggtcccaga agatccagtt atgatcgcca ggtccttgtt caaaggtgac
     6481 cgccaagcct tgttcctgca tagcagatac catttcctga tttgcctgat aaagaaaatc
     6541 agattcccca caagccatat aaaatcttgg aactggtttt ccgcttttta ctagttcttt
     6601 caataagtat tgcggatcat tttttgaacc ttcgatttga tccaatggtc caaagatccc
     6661 ttcccaaaaa gatgctttac gaaccatcaa aagatcatcc attcgatttc ctacagaaac
     6721 agcgcctgaa agagagatta ctgctgcgaa attttctgat tttgcaaggc ctagttttaa
     6781 cgcgccatat ccgcccatcg ataatccagc ggcatatgtt ttttctcttt ttcttgttaa
     6841 ttgcgggaat aattcatgac aaatctcagg tagctcttct gcaatgaatg tccaatactt
     6901 catgtcataa actgtatctg tgtaccagcc aagatcagta gatggcatga tgacagctaa
     6961 tccgtagttt gctacataac gttcaatcga cgtacgtctt tcccatacac tatgattgcc
     7021 acccattcca tgaagtaaat ataaaacagg tatatccgtt gtctcttgct ctgaactggt
     7081 gccaatcacc ttttttgttg tttgcggtag tatcacattt agattgactt ccatttctaa
     7141 cacattcgaa taaatatttg cttgaataaa tgccatattt tcgccttcct tagtaagaag
     7201 ttatttttga ataactttaa atttgaatga ttcggctacg tacatatctt aaccgaaact
     7261 actgttttat tttaccactt ttttcatgaa cgaaaagcgt taagaccaaa gcaatgatca
     7321 cgagtaacaa gccaactaga taaacggaat gtaacccatc ataaagaatt tttcttaatg
     7381 gtaacaaaat atccgcatca atatatttag ccgtttgtgg attcaccagt ttattcatga
     7441 tatctgcatc atttgttaaa tttgctgctg caagttctcg tttagttacc ccatttatca
     7501 ataaaccaaa aatagaaacc atgactgttt gaccaattgt tcgaactaac gtattaaaag
     7561 aagttgctac accaagctgg tcacgagata cactattttg tgcactgaca gtcgaggtag
     7621 tcatggtcac acctaagcct ataccgatga ccgttgatat agcaaaaaac cataaatagt
     7681 tacttctgaa aggcataaga tacaaccaag cgcccccaat agctgcgatt agtagtccaa
     7741 ttctcaagac ccaatgagtt ttcatttgag tcatccactt gcttgcaagg aaagaaccga
     7801 ccatccagac tagtgataat ggtgctaata ccagcccacc aattgctgca ttcagcccta
     7861 atacaccttg catccacata gggatataaa catcgattcc catcaaaaaa ccactagcca
     7921 atgctgcagc caagttgacc acaacaaata gtggattttt gaataaatgc atcatgatga
     7981 caggatcttt agctcttttt tcggcaaaaa caaaaatacc taacattacg atactaccta
     8041 gcagcaaagc agaaacaagc ggactcattc cttcgtctcc taataattgg aaccctaaca
     8101 gcaatgagac aagcatggcc atcaaactaa aactaccaaa aatatccatt ggtttagact
     8161 catgtatgat ttttggttca tttaaatata gccaaatcaa aataattaat agaattccga
     8221 tcggcacatt gataaaaaag atccagtgcc agctgatcgt ttcaacgatg aatcctcccg
     8281 caagcggtcc aaaaacactg gcaatccccc aggctgaact attcaaacct aaaacttttg
     8341 ctcgcttttc aatagaataa agatcgccga tgatcgtcaa agctactggc atcattgccc
     8401 cagcacccat cccttgaatc gcacgagcag caatcaaccc aatcatgctt tgagataaac
     8461 cgcaaaataa cgaaccgata ataaacaata atgtcccaaa aataaagatt ggtttccgtc
     8521 ccactttatc cgcaagtttt ccgtaaatcg gagtaagcat agcgtttgtc agcaaataaa
     8581 tcgaaaatac ccagttcatg atttccattc catgtaatga accgacaatc gttggcatag
     8641 cggttgttac gattgtacct tcaatagccg tcatgaacgt tgcaatgaaa atactcactg
     8701 tgaccaaagt aacatttgtt tcttttttca tcttattcct ccctttcatt atgacaggat
     8761 ctaaaaatag ataaataatt actatctttt tcaagaggtt aactgccata aaaaaagctg
     8821 gaacaaccac cacttggcga tcgttccagc tgaatataac ttattttgct aaacttgctt
     8881 tcaacgcatc tactttgtcc gtatgttccc aaggcaaatc tacatctgta cgaccaaaat
     8941 gaccgtaagc agctgtttgt ttgtaaatcg gacgacgcaa atcaagcatc tcgatgattc
     9001 ctgctggccg aagatcgaag ttttctctca ctgcagcaat cagttcttct tccggaaccg
     9061 ttccagtacc gaaagtattg attgagatcg aaacaggttg agcaacacca atcgcataag
     9121 ctagctgtac ttctgctttg cgagcaagcc ctgctgcaac aatgttttta gcaatataac
     9181 gtgcagcata gctggcagaa cggtcaactt ttgtcgcatc tttaccagaa aaagcaccgc
     9241 caccatgacg agcataaccg ccatacgtat caacaatgat ttttcttcct gttaatccgg
     9301 catccccttg aggaccacca atgacaaatc ggccagtcgg attgatataa tatttcgttt
     9361 gatcatctaa taattcagct ggaataactt ccttcactac tttttcgatg acatcatgac
     9421 gaattgtttc attatctact gcatcatcat gctgtgttga aataacgatt gtatctacgc
     9481 gttccggttg tccttgatca tcatattcaa ccgttacttg agattttgca tctggacgta
     9541 aatacgttaa ttcatttgat ttacgcaaat ctgccaagcg tcgtaccaga cgatgactca
     9601 aagcgattgg taaaggcatc aattctggtg tttcatctac agcaaaccca aacattaaac
     9661 cttggtcacc agcaccaatc tcatctaata cgtctttttt atcttcatcc cggatctcaa
     9721 gtgcttcatc aactccttga gcaatatcag gagattgttc atcaatcgca accaatacag
     9781 ctgctgtatc tccatcaaat ccgaattttg cacgtgtata accaatttct tttattgttt
     9841 cccgtacgac tttttgaata tctacatatg ccgtcgtaga tatttctcca aatactaaaa
     9901 caagaccagt tgttacagac gtttcacacg ctactcgtgc tgttggatct tgttttaaaa
     9961 ttgcatccaa gattgcatca ctgatttggt cagcaatttt atctggatgt ccttcagaaa
    10021 cagattctga tgtaaataag tgtctttcta ccattataat gattccccct agttaaattc
    10081 ggttacaagg catctccgtc ctgcggatcc tttcgggaac ttgcaacgaa ccaagtatag
    10141 cagataaaac agttttctgc tcgtattttt tcattttttt gcttaaaaaa tataaaaata
    10201 ttttacatta gaactgtcca aaaaagctta gcacaatcat ccataaagat aactctgcta
    10261 agctttttaa aatcattctt tcagataaaa agaaaaagat actcaaaaag tatcttccat
    10321 gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag ggcggtgtct taaccgcttg
    10381 accaacgggc caataaaata aatgggccta aatggactcg aaccatcgac ctcacgctta
    10441 tcaggcgtgc gctctaacca gctgagctat aggcccataa aaactaaaag cgggtgacga
    10501 gaatcgaact cgcgacaaca gcttggaagg ctgtggtttt accactaaac tacacccgca
    10561 taatggcgcg ggacagaatc gaactgccga cacatggagc ttcaatccat tgctctacca
    10621 actgagctac cgagccaaac ggttccgacg ggaatcgaac ccgcgatctc ctgcgtgaca
    10681 ggcaggcatg ttaaccccta caccacggaa ccaatgtttt tagtttttaa ttgcgggagc
    10741 aggatttgaa cctacgacct tcgggttatg agcccgacga gctaccagac tgctccatcc
    10801 cgcgataata tgaaaaagga ggataaggga ttcgaaccct tgcacggttt tacccgcctg
    10861 acggttttca agaccgttcc cttcagccgg acttgggtaa tcctccatgt aaaactatat
    10921 taaattaatg acccgtacgg gattcgaacc cgtgttaccg ccgtgaaagg gcggtgtctt
    10981 aaccgcttga ccaacgggcc aggtaaaaca aaatattgaa acggagaagg agggatttga
    11041 accctcgcgc cggtttcccg acctacaccc ttagcagggg cgcctcttca gccacttgag
    11101 tacttcccca aaaccaataa gattttgtac taatgggcct aaatggactc gaaccatcga
    11161 cctcacgctt atcaggcgtg cgctctaacc agctgagcta taggcccata aaaaactaaa
    11221 agcgggtgac gagaatcgaa ctcgcgacaa cagcttggaa ggctgtggtt ttaccactaa
    11281 actacacccg catggcggtt ccgacgggaa tcgaacccgc gatctcctgc gtgacaggca
    11341 ggcatgttaa cccctacacc acggaaccag tgtttttagt ttttaattgc gggagcagga
    11401 tttgaaccta cgaccttcgg gttatgagcc cgacgagcta ccagactgct ccatcccgcg
    11461 ataatatgaa aaaggaggat aagggattcg aacccttgca cggttttacc cgcctgacgg
    11521 ttttcaagac cgttcccttc agccggactt gggtaatcct ccatatgaaa cttacaacgc
    11581 cacaatggac cttgtaggac tcgaacctac gaccggacgg ttatgagccg tctgctctaa
    11641 ccaactgagc taaaggtcca ggctcttaaa aatcgcggcg gaggggatcg aacccccgac
    11701 ctcccgggta tgaaccggac gctctagcca gctgagctac accgcgaaaa agatattcat
    11761 tatttaaatg aaatggagcc tagcgggatc gaaccgctga cctcctgcgt gcaaagcagg
    11821 cgctctccca gctgagctaa ggccccgtgt ttaaatttat aattatcggg aagacaggat
    11881 tcgaacctgc gaccccttgg tcccaaacca agtgctctac caagctgagc tacttcccgt
    11941 ttaattaacg cgcccaagag gagtcgaacc cctaaccttt tgatccgtag tcaaacactc
    12001 tatccagttg agctatgggc gcatatttga aatgccgagg accggaatcg aaccggtacg
    12061 gtgatcactc accgcaggat tttaagtcct gtgcgtctgc cagttccgcc accccggcgc
    12121 gattgctttg gcaaagcgga aaacggggtt cgaacccgcg acccccacct tggcaaggtg
    12181 gtgctctacc actgagctat ttccgcgtgt ttaaagatgc cggctaaagg acttgaaccc
    12241 tcgaccctct gattacaaat cagatgctct accaactgag ctaagccggc atgtaaaact
    12301 taaattatgc gggtgaaggg acttgaaccc ccacgccgta aggcgctaga tcctaaatct
    12361 agtgcgtctg ccaattccgc cacacccgca aaaatatgag ccgtacaggg ctcgaacctg
    12421 tgaccctctg attaaaagtc agatgctcta ccaactgagc taacggctca tatggaggtt
    12481 aacgggatcg aaccgctgac cctctgcttg taaggcagat gctctcccag ctgagctaaa
    12541 cctccatgga taactt
//
LOCUS       DANJMI010000075        12470 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000075 DANJMI010000000
VERSION     DANJMI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.075"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(195..1961)
                     /locus_tag="PWU81_002636"
     CDS             complement(195..1961)
                     /locus_tag="PWU81_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1111275.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1921..3675)
                     /locus_tag="PWU81_002637"
     CDS             complement(1921..3675)
                     /locus_tag="PWU81_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1111276.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3785..4354)
                     /locus_tag="PWU81_002638"
     CDS             complement(3785..4354)
                     /locus_tag="PWU81_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HDL1111277.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4372..5787)
                     /locus_tag="PWU81_002639"
     CDS             complement(4372..5787)
                     /locus_tag="PWU81_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL1111278.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(5798..6466)
                     /locus_tag="PWU81_002640"
     CDS             complement(5798..6466)
                     /locus_tag="PWU81_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL1111279.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6596..7180)
                     /locus_tag="PWU81_002641"
     CDS             complement(6596..7180)
                     /locus_tag="PWU81_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1111280.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(7511..7744)
                     /locus_tag="PWU81_002642"
     CDS             complement(7511..7744)
                     /locus_tag="PWU81_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HDL1111281.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(7759..8709)
                     /locus_tag="PWU81_002643"
     CDS             complement(7759..8709)
                     /locus_tag="PWU81_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL1111282.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(8706..9548)
                     /locus_tag="PWU81_002644"
     CDS             complement(8706..9548)
                     /locus_tag="PWU81_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL1111283.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(9550..10269)
                     /locus_tag="PWU81_002645"
     CDS             complement(9550..10269)
                     /locus_tag="PWU81_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL1111284.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            10961..11119
                     /locus_tag="PWU81_002646"
     CDS             10961..11119
                     /locus_tag="PWU81_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1111285.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            11191..12417
                     /locus_tag="PWU81_002647"
     CDS             11191..12417
                     /locus_tag="PWU81_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL1111286.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
BASE COUNT         4319 a         2204 c         1670 g         4277 t
ORIGIN      
        1 taactggttt atcgccaatt ttttaaaact ttgattattc cttatattat aagaatagac
       61 attaagtgaa ctaattcaat catactgatt ctttcgtgta ttcaaaatta gatgctgttt
      121 aataaaatta ttctttttga aaaagaatta taactatatc ccaaaaattg taggttaatt
      181 taaagcctat ttcatcatcg tgattgagct tctttttcat agacgaattt ctcagatagt
      241 ttagttttct ttatgagatt tttgtatatt tctgaccttc gatacaactc attatgagta
      301 ccttgatcaa ctagttttcc ttgatccatc actacaattt tatttacctt ttcaatagac
      361 ttcaagcgat gcgatataat tagtactgtc ttatcttgta ttaaacgatt taaactttcc
      421 tgaatacgtt tttcattttc cgcatctaaa ctggcagtaa tttcatctaa aattaaaatt
      481 ggcgcatttt tcaaaaaagc ccgtgctatg gataggcgtt gtcgctctcc tccagaaagt
      541 gaacttccgt tttctccgac caatgtatga taaccgtcag gtagtttttc aataaagtct
      601 tcgcagttag ctaaacgagc tgcttttttc acttcctcat ctgacgcttg actatttcca
      661 atacgtatat tttccaaaat acttgtatta aacaaagtta cctcttgaaa aacaatagcg
      721 attttgctaa acaaagaagc tggagataca cgtttaatat catacccatc aattaaaata
      781 cagccttcat cataatcata cagtctagac actaatttca agatactagt tttcccggaa
      841 ccactagcac ctactaacgc agtcacttct ccttgtttag ccgtaaaaga gatatgatct
      901 aaaattgggg tattgttatc atatgagaaa gagacatctc gcaactcaat gtcaaaagat
      961 ttcagaggac tatctgatcc ctcttggata ctggtttcct tcatagctct aattcgctgt
     1021 attttaggag ctaaataaaa tatttcaagc actgcttctt tcatagaatc gaaggaatcc
     1081 tttattttta ttgcggctag taagtaacca acgacgtaga gtatagtcac ttcacccgtt
     1141 aacagtaagt gaacacctac tagaatgact actgctaaac taacatatga gaaaatagag
     1201 gataaggcca tgactgaaaa agtacttaat tctaccttta aatgaatcct ttcactctct
     1261 tccatttttt tgaaaagtct gtcttgaact tttttagata gattaaagct attaatctct
     1321 tgctgcaatt ctatagtttc ttgaaattct tccgagttct ctctcaaagt atcgtaatat
     1381 ttagtattgg ctttcgtttg ggatttcttt gataacaaga ttaagacaaa actaaataac
     1441 gttggcaaaa taacagctaa tcccattttg acattaccaa ccaaaagcat cactgaaata
     1501 aaagggaaaa acagagccat accgccggct ttaggtattg catgactcat cgcatgctca
     1561 ataccttcaa cgtcagacat gatagtttga gataaatctg ataaattatg ttttgaaaaa
     1621 taggataatg gtaaatttga caaatcgtct gcaatttgca ctcttaaatg cgcactttct
     1681 ttataagtac tattatataa gttttcatac tccctatcta acaaccaata catgaaaagc
     1741 aaagtaacaa acgatacggc aaaataaagc caacggggct ttgtattttc taaaactaat
     1801 tcgtctatca gcatcattat aatgaatgct ggtaccatat taatgcagta tacaaaaaaa
     1861 ctagaaatag aagctttagt taaagcttct gcgccctgat cacttaaagc aaatttgttt
     1921 ttataaaaac ttttcattgt caagcctcca atcatttgca ttctcataat tagtgaccaa
     1981 ttgtttatac acttgatgtt tagccattaa ttcattatga tttccacgtt ctactatttt
     2041 cccttctgac aagacaagaa tttcatctac agatttaatg ctggataatc gatgagctat
     2101 cataataact gttttttctt tcatcagatg ttgaaaagcc ttttgtagtt catactcatt
     2161 atctggatca atagatgcac tggcttcatc cataataaca atctttgcat cttttaaaat
     2221 tgctctggca atagctatac gttgtttttc tcctcctgac agataaactc ctttactacc
     2281 aattaaggta tgctctcttt cgggaaattt ttctaaaatc gattcgcaac cggctaatct
     2341 tagcgcgtca aatactttac ttgctggcgc ttgaggattt gctatagaaa cgttatcgta
     2401 aatactctga ttgaatagtt tcgtatcttg aaagacaaaa gcaattgtgt taataattgt
     2461 gtcttttgaa tagtgattta aaggcttacc accaatttta atctgtccct tatccacatt
     2521 gtagaaacct gaaattagtt tagctatcgt actctttccg cttcctgatt ttcctactaa
     2581 tgcataaatt cttttttgtt ctaaaactaa gttgaaattt tctaaaacac actcttcttt
     2641 gtatgaaaag ctgacatttt caaatgcaat gtcacaattt ttaatctgtg tctcgttccc
     2701 atattttagt tggtcttgct tcatttctaa ataaagggag tctagtgttt cgactgcata
     2761 tcttgcttca aacaagtgca tacttacata cattattctc atgaaagaaa caaaaataat
     2821 tccacttaaa aaaaacaaca tcaacaagtc aacagctaat atcttgggag tggtaagaga
     2881 agttataaaa aaggttatag gaactatcaa aatgcaaact atgccaaaaa atagccattg
     2941 aaaaagaaca aaaggtgttt tcccactcaa agaatactta tatgcaaact tagcataatc
     3001 gttaattgct ttattcaagg ctttaaatga tgttacttta gcaccaaata tctttataac
     3061 ttgaattccg cggatatatt ctaccgtttc agagcttaac acatctaacg attcttgata
     3121 tttttgaata aaatttgtat tctctgtcat tttctttaac aagaatacac caattactaa
     3181 catggcgatt agaaccaatc caattttaaa gttaatgaaa aagcctaagg caactgataa
     3241 aacaggtgtt actatggctt gagcactatc tggaatcaag tgagcaattg ccatatgtgt
     3301 tttagccgcg ttgtcatcaa ttgtctttcg aatatatccc gaagaattta aatcaaaaaa
     3361 acgaaaactt gcttctgtta aaccgctaat tccctttttt ctcaaattag tctctaagcg
     3421 aaacccaagg atatgagaaa ataagccaga aaaaatataa aataaagctc ctaaagttaa
     3481 ccaagcaaca gtttgaaccg cataactctt agctaaatat tcatctccta caataattag
     3541 agactgaaga aatttgtaaa tactgtaata tccatagact gttaatacga cagaaaaatc
     3601 agaaaataaa gtggctaacc atccgtagta tttcatttta gggacataac taaataattt
     3661 tttgtatact ttcatttttt cctcccccgc attcaatttc ttctcttatt cgtttacaac
     3721 tgttaatgct ttccgtaata gcgtaaatta gagatgtaca aacagataaa aggtaaacta
     3781 cgttctattt tttgtatttt tttaagactt ttcttccaat caatacacca atcatagata
     3841 aacttgctac aactaacact gaaatgattc cccatatacc taccgcatag tggatcattg
     3901 catccattgt tgcagcagat tgtcctttat ctatccaatc ttgacgaaaa cgatctgtaa
     3961 agaaccatat aggaatccac tgtccggcag cactgcctaa ttgcattaga gcccaagacg
     4021 caacttgagg aattgctcga tcatatccaa atacagatag aataatatca gctaagatcg
     4081 ccatactaat cgaactgata aaccaaggta agtacgcccc gcccattaac gtaaaaaata
     4141 acaataaaac aattgtgtaa atagtaaaaa ttccttttct agggtagtta tagctaatat
     4201 aaacaaatat gattccagat aataatccaa aaatcccggg ggatatgctg tgaccaaacg
     4261 ccccaccaat actactaacc attattgaaa aaaagtaaat tactagggaa ataactgtag
     4321 ctaaaccaat aaaaatataa tcttttgttt tcatatatat tctcctcaat tctatttcgt
     4381 tatgtcatta aaaatctgtt ctatcaaagt tgaatcttct actctaagtt ttatatcttt
     4441 ttttattcca ccatttttta aataaataat tctattgcat gttttataaa gaaattccgg
     4501 atcatgagta gctataacta ctgcaccctt ttcttttaag tctttaataa ggtcagcaat
     4561 cctttccata ttaatataat ccaacccact tgtaggttca tcaaatagaa ataaattagt
     4621 ttttgacaat gaagctatag aaaccaataa tctttgtttt tctccaccgg ataaatcaaa
     4681 cggtaattga tctttcattt tataaagttc catttttttt aaaatttttt cttgctgatc
     4741 tttactaact atttcattcc ccagggctaa ctcgctcctc actgattctg tgaaaaattg
     4801 gtaatccatt tcttgcatta ctaaaaacgg cgatgacaaa ttttttttat tttcgattat
     4861 ctttcctttg gtagggcgaa taatcttcat gagtattctt agtaaggttg ttttccccac
     4921 accgtttttc cctaccaaac caataacatc tccaactttg acatcaaaat ttatgtcttt
     4981 tagaatattt tcataagtaa tttcttcaac ttttaataca tctgcactta ccttttcgat
     5041 atagggaact tcaatcgaac tattaaaaat gtcaaatttc cttaatgcgt tattgttttc
     5101 agtttttaac aactgaatag ttgtagtttt tacagttcta tttttaacaa atagaacttg
     5161 atcaattaat tctttcaaat agtaaaaacg atgttcagct ataacaatag taaatccttt
     5221 attttttaaa tcacaaagta agtcagcaag tttcttagtc gctttgaagt ccaaatttga
     5281 agacggttca tccaatataa ttaatttaga gtttagcgat aaactacatc caatagaaat
     5341 tttctgccgt tcaccacttg atagcgaaaa aatgttttta tctaatatat ttgaaagatc
     5401 taataattca gctaattcat tgattctttt atccatatgc tcttttgtaa atccaaagtt
     5461 ttccatcgaa aatactaact cactcgttgt atttgttgta aagaattgtc ctctaggatc
     5521 ttgacttacg tagccaatat tttttgatat actttcttgt gttaaggatt gtagcgagtt
     5581 attttcaaat aaaatttccc caaccaattt tccgtcataa agtcttggaa taagtccatt
     5641 cattaacttt aatagtgtgg atttcccact cccagaagaa ccagtaatca atgtaagact
     5701 gccttttttt attgaaaaat taaggtcaac aagcgcttgt tccttaacat ccttatatga
     5761 aaaagaaaca ttgttaaatt caaacaattc tttcacctca aatcaaaata cttaagaaaa
     5821 aaatagtaac ggaaaacagg atgatatagt ctaataagct catgtttgtt ttatagtaag
     5881 aacttctttt cacaggagat tctatcccct ttgttattgc attagcggtc atttcttcag
     5941 caatcatagt tgcacgtata agaacgggaa ctaacagaaa ttccaaatat tgcagaggct
     6001 gaaaaaaaga caagcgaatg tttctcattt ttaaagattc tttaatcatt cttatttcag
     6061 aacgataagt cggaaagaat ctgatcacta ctgtaatact taacattatt tttttcggta
     6121 aatgtatttt ttctaaagag gataataatt cattgctagt atagacacta accaacaacc
     6181 atgagatcat tacgaccggc aacattcgaa atacaatata taaggatgtc gcaaaaaata
     6241 tagtaaaaaa ctctatcttg atagcatcaa taattttcat tactaaaaac agaactgaat
     6301 aaatcaagat gaaataaata catgtcttta ttttacgaaa catcaatact aaaactgtag
     6361 caataatgaa acaccccact tgaaatatat ttccctttcc taaaaaaata atgaccggaa
     6421 aaaaaatatt taaagtaatc aaggttaacg gattgatttt tgacactctt tcacctccaa
     6481 aaaataagat cgcactgtta tcttaagctg ataaaaatgc accgttattc agataacagt
     6541 gcgatcttat caaaaagcat aactgagaac aattctcaat gcaagctttt tttaattaac
     6601 tcagtaattc aatccaccca ggaacagaaa attgatacaa tcttctggtt aatagcatag
     6661 tcttttcttt tgaatagcta tgcttcaata tatcatagaa tgtagcgtag aatgagtaaa
     6721 tgtagaagtg gatttcttct tcattaaaga ctaaattttg attgatcatt ccgatagtct
     6781 ttaacttatc tagacaacag actgtctttt tcgtcacaaa tgatgtaatt ttttcaacaa
     6841 tatcttgata tttggttcca gctgattgga ataaaatcaa atcgaatata cgtttattct
     6901 caaaaatata attaataaat ggatctaatt cattgaagtt ctctctccaa cttttctcta
     6961 tatctatatt ttctaagttt aagaggtatt tggtaaactc ttcattatac atattaataa
     7021 gcgcattaat atgcggctga ataatttctt caaataacgc ttccttagaa gtaaaatgtt
     7081 tataaaaagc acccgtagtt acgcctgcgg tcaggcacag ttgccttatc ttagtttgct
     7141 gaaatccttc tttttcaaaa tggcttagtc cactttctat taacttttgg tgagttataa
     7201 tataatctct ggtttctatg ggtacttcct cctaataaga tgaattaact ttattatctt
     7261 acacaggact tgaagtttca atcgttaacg tttattactg aactcattcc ttagagctaa
     7321 actaatgaga aagagcagaa taactgatac aaaaagcaat cctacaagaa ttaatagact
     7381 catctaaatc attcaccttt tctaaaataa tccgagaaag atttaaaaaa attatatcca
     7441 gctattagga taagtcctat cataatcact ccaatagcca ttttcttatc ctccctaaaa
     7501 ataaagtctt ttacttaaaa ccttttttgt aatcgtcaat aaattttttc cctttatcag
     7561 ttaattgaaa tcctatatct aaatagtcct catatctaag tttagccagc atctcactta
     7621 cagatgctgg actaacactt agaacttttg ctaaatcatt attttttaat ttcttttttc
     7681 tttttgataa ttcctgaatt gctagtaaat aaattagtat agtagtcgtt ttaatttgta
     7741 ccatacctac cctccaaatt atttcgaaag gccttcagca attttatcca ggttccattt
     7801 catcatcgca tagtaactat caccatcttg tcctttttct gcaattgaat cagtaaaaat
     7861 cgttgaatag ataggaatat tggtatcttt tgatactgtt ttcattggtc tatcatccac
     7921 actgctttct acgaataagg agggaacttt tgttgtgcgt aatttttcaa ctaagtgttt
     7981 tatttgatct ggtgttcctt cttcttcagt gttgatttcc caaatgtaag cagaaggcac
     8041 attatacgct ttcgagaaat atttaaagca tccttcactt gtaacaatca tttttttatc
     8101 attcggaatt gaagcaaatt tagatttagc ttctttgtct agagaatcca atttttcaat
     8161 atacttatct agattttctt tatagaattt tttattatca ggatcttttt ccgctaactg
     8221 tttttcaata tttttagcgt aaataatacc gttttctaaa tttaaccaag catgaggatc
     8281 ttccttccct ttctcactct gaccctctaa gtaaataaca tctatgccat cacttgctgc
     8341 aaaatagtct ttgttttcct ctttgttcgc atttttaact aattttgtaa accaagcatt
     8401 tcctccagtt tccaagttaa caccgttata aaaaatcaaa tctgcttttg aagttttttg
     8461 aacatcttca ggcaaaggtt catattcgtg gggatctttc ccaataggta cgatactgtg
     8521 taaatcaatt ttatcttttg ctatattttc ggtaatatct gctaaaatcg aattagtaac
     8581 tactactttt aatttttcgt tcgaagccac tttatctttg tctttactat ttattgaaca
     8641 tgccccgaat ataaaaagag caatcaaaat ccccagactt cccagtactg ctttccattt
     8701 tctcatcata acgatctctc cttctttctt aaaaattttt gttttggtga gattagaaaa
     8761 ctaattacaa ataagaatgc cgctgttaaa acaatacttg atcctgctgc tatgttaaaa
     8821 ctataaccaa taaacaatcc aagcaatgat gaacttgctc cgactgttgc agaaataaat
     8881 atcatagttt tcaagctatt tgcatataaa tatgctgtag ctgctggtgt aattaacatt
     8941 gcaacaatta atatagttcc cacactttgc atagctgtta ctgctactaa agttaataga
     9001 accatcaata gataatgata aaaactgaca ttcatcccta ttgcttttgc cattaatgga
     9061 tcaaaagaag tgattaataa ttctttaaaa aatagaacta ctataaccaa cactaaaacc
     9121 gaaacgccaa ttgtaatcca cttatcaata tcttgtacag ctaaaatatt tccaaataaa
     9181 atatgaaata aatccgttga gctatttgca actcctataa ggataactcc tagtgctaaa
     9241 aaagaactga aagtaatacc aattgctgta tctcctttta ttacactatt gctttttata
     9301 aaagtaataa tgatagaagc taatagacca aatacaatcg caccaataaa aaaattaata
     9361 cctaaaatat aagaaattgc tactcctggc aaaacagcat gagaaatagc atcacccata
     9421 agagacattc ctcttaaaat aataaaacaa cctactgttc cagaaacaat ccctataact
     9481 acagatgtaa ttagagcatt ctgtaaaaag tgaaagtcat acagaccatt aataaaattt
     9541 tgaagcatat tattccccct ctccaataaa tatagtatct ccataggctt tttttaaatt
     9601 ttcttcatta aatacagatt ctacacttcc gtgagcaatt agatttcgat tcaaaagaat
     9661 aatattatca aaatacttct ttaccttgct taaatcatga tgcacaatta gcactgtttt
     9721 tccctgatgt ttcaattttt ttaacgtatc cataattatt cgttcactaa ctaagtctat
     9781 ccctacaaat ggttcatcta aaaatatgaa atccgcattt tgagcaagac atcttgctaa
     9841 aagtacacgt tgaaattgtc ctccagataa ttctcctatt tgatgattag aatattttcc
     9901 catatcgacc ttttccaaag cctttgaaac tctttgccat tctgcatttt taattctttg
     9961 aaaaactttc attccggcat atgttcccat agatacacat tcttttactt ttattggaaa
    10021 agtaaaatca atatcagctt tttgctctac atatgcaatc ttcaccagtt ttttattgat
    10081 atcttcatta ttaatggaga cctctccctg atgagggata atatttaaca ttgctttaag
    10141 caatgtggat tttccagcac catttggacc aataatacct gttaaagcac cttcttgtat
    10201 ttttaaagaa atctcgttca atgctaaaaa atcgttataa caaacattaa gctttttcac
    10261 ttctaacata actttctcct tctaaatgat aatgattatc acgatcatat ggtatacaaa
    10321 aattatattt tagtcaaccc taaattttat agtttagatt tttttctgtt gctgaagatt
    10381 agaatgacaa aactatttga aactacgcgt atagtaagta gctttcaaaa tatataaaaa
    10441 aggagcgttc tcaaatgaga acatgctcct aaaactactt tattggcgta tggaaaaatt
    10501 atacttgaag tggatagcgg gttgaataag ttgataagca tcaaaacatt tataaaaatt
    10561 attcaagact atgtctgttc catatacaaa tatgaaaatc agtaaactcc atttaacttt
    10621 tttcgtagtg tgtcttttta tacatgttta tcgttattaa tagtgaatag gaaaaaaact
    10681 atttttgatc attgacaatt aaatacaatt tttgaataac gatccctgct attcgagcta
    10741 gaaactcaag tacgccatga cctgattagc gacaaatact tagagatttc tttttgttct
    10801 gggaaaacaa aatgccatga caatagcagc aaataatgat acttccgtaa ggctcgccgt
    10861 aaactgggga gaggtgaaat tcctatgtgc ttacccaaag caattcaaaa atttcataac
    10921 tactatttta ggagatgatt ttatagaatc cagagtctac ctgaataaaa aacaaacctg
    10981 tgcatttcta ggaatcgata ataatactct agataagtgg attcgtatat ataatttccc
    11041 agtaataaga gttggtggtg tagttcgctt tgacgtagaa gatattaaag actttatgaa
    11101 tacatttaaa ataacttgaa attttcttct cgccgcatat actaaaatta gtgaaatata
    11161 ctgccttact ttttttaagg agtgaggatt ttgcctacaa agctttcaaa tgggaaatat
    11221 aaaactaatc tgagatatcc aaaaaggttt aaagaaataa caggaattgc atcagaaaag
    11281 tatcaaaaaa catttccaaa taggcaattg gcgattaaag ccgaaaatga tatgaagaag
    11341 aaaatagaaa aagttttgag agaggagaat gcaaactcat tagaattaaa aggaaaaata
    11401 acgtttaaaa aattttacga atctaaatgg cttcctagat atgaattagg tcaaacaatt
    11461 agatcaaaca ggcctcctag tgatataacc attagtaata caaaagatat atttcggttg
    11521 catattttac ctatgtttgg tgaatacgca atgaattatc taaatatcaa tactgagata
    11581 atatcagatg aattaaccaa aaaatcgaaa gaatatgcaa atataaaaat cataaaagga
    11641 tatgttcgtt ccatgttcga tattgcagaa atactaaatt atatcgaatt taatcgaact
    11701 actaaaatta ttcaaagtat cactgcacct aaaaaaaatg ctttagaaga aaaaagaata
    11761 caagaaggta agcaagcact atcatcaaaa gaacttacta attggataga agcagtaaat
    11821 gatgatttta ataatcattt acttactttt catgattaca cactttttat gattaccctg
    11881 tatcttggtg atagaaaaag tgagacttac gctttgcaat ggaaatatat tgattttgaa
    11941 aaacaaacgg tccgtcttaa acatacttta gataaatatc aaagaaaaaa gtttacaaaa
    12001 ggtcgaaaag ataccgtcat tcaagtacca gaagttgtaa tgacattgct aagtgaatgg
    12061 aaatcggtac aagctgatca attgttgaaa ttaaaaatta agcaaacgtt agatcagtat
    12121 ctctttactt atactaaacc atctggagaa gtaaattgtc ctgttcatgc cgattaccta
    12181 aactatcgaa tcaattcaat taaaagaagg catcctgact tagttcattt atctccacac
    12241 aaactccgtc atacttatgc aactatagct agacaaggtg gagcagatat gaatcaaatt
    12301 tcaaatgctt taacacactc agatatttct acaacaaaga tatatgtgaa cacaccagat
    12361 atagttgata aggctgtttt tgaagctttt cagagaggat tgaataagtg tgattgatat
    12421 gtgttcttaa atgtgttctt aaaaacaaaa gaagcacttt ccaaataatt
//
LOCUS       DANJMI010000076        12135 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000076 DANJMI010000000
VERSION     DANJMI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.076"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..610
                     /locus_tag="PWU81_002648"
     CDS             <1..610
                     /locus_tag="PWU81_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL1111287.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="PWU81_002649"
     CDS             723..1001
                     /locus_tag="PWU81_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL1111288.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="PWU81_002650"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="PWU81_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL1111289.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="PWU81_002651"
     CDS             1458..2213
                     /locus_tag="PWU81_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL1111290.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="PWU81_002652"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="PWU81_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDL1111291.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="PWU81_002653"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="PWU81_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDL1111292.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="PWU81_002654"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="PWU81_002654"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL1111293.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="PWU81_002655"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="PWU81_002655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDL1111294.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="PWU81_002656"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="PWU81_002656"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL1111295.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="PWU81_002657"
     CDS             6950..7492
                     /locus_tag="PWU81_002657"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL1111296.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /locus_tag="PWU81_002658"
     CDS             7515..9071
                     /locus_tag="PWU81_002658"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL1111297.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="PWU81_002659"
     CDS             9085..9987
                     /locus_tag="PWU81_002659"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL1111298.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="PWU81_002660"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="PWU81_002660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL1111299.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="PWU81_002661"
     CDS             11430..11852
                     /locus_tag="PWU81_002661"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL1111300.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4035 a         2155 c         2677 g         3268 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttatt
//
LOCUS       DANJMI010000077        11908 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000077 DANJMI010000000
VERSION     DANJMI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.077"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..465
                     /locus_tag="PWU81_002662"
     CDS             <1..465
                     /locus_tag="PWU81_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL1111301.1"
                     /translation="WKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKKRDTQAAYAF
                     LKRLVKQFDEPKVVVTDKAPSIASAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVK
                     RRNKFYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPD"
     gene            complement(1080..2120)
                     /locus_tag="PWU81_002663"
     CDS             complement(1080..2120)
                     /locus_tag="PWU81_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL1111302.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2469..2795)
                     /locus_tag="PWU81_002664"
     CDS             complement(2469..2795)
                     /locus_tag="PWU81_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111303.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2782..3585)
                     /locus_tag="PWU81_002665"
     CDS             complement(2782..3585)
                     /locus_tag="PWU81_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL1111304.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3938..4228)
                     /locus_tag="PWU81_002666"
     CDS             complement(3938..4228)
                     /locus_tag="PWU81_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL1111305.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4364..4543)
                     /locus_tag="PWU81_002667"
     CDS             complement(4364..4543)
                     /locus_tag="PWU81_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111306.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4540..4890)
                     /locus_tag="PWU81_002668"
     CDS             complement(4540..4890)
                     /locus_tag="PWU81_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111307.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4883..6208)
                     /locus_tag="PWU81_002669"
     CDS             complement(4883..6208)
                     /locus_tag="PWU81_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL1111308.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6658..7272
                     /locus_tag="PWU81_002670"
     CDS             6658..7272
                     /locus_tag="PWU81_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1111309.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(7437..7679)
                     /locus_tag="PWU81_002671"
                     /pseudo
     CDS             complement(7437..7679)
                     /locus_tag="PWU81_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7883..9202)
                     /locus_tag="PWU81_002672"
     CDS             complement(7883..9202)
                     /locus_tag="PWU81_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HDL1111310.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(9357..9944)
                     /locus_tag="PWU81_002673"
                     /pseudo
     CDS             complement(9357..9944)
                     /locus_tag="PWU81_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10582..10839)
                     /locus_tag="PWU81_002674"
     CDS             complement(10582..10839)
                     /locus_tag="PWU81_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL1111311.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(10832..11101)
                     /locus_tag="PWU81_002675"
     CDS             complement(10832..11101)
                     /locus_tag="PWU81_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL1111312.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(11232..11411)
                     /locus_tag="PWU81_002676"
     CDS             complement(11232..11411)
                     /locus_tag="PWU81_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111313.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
BASE COUNT         3675 a         2151 c         1826 g         4256 t
ORIGIN      
        1 tggaaaatgg atgaaacgta catcaaaatt aaaggaaaat ggcattattt gtatcgagcc
       61 atcgatgcag atggtttaac cttggatatt tggttacgta aaaaacggga cacacaagca
      121 gcctatgctt ttcttaagcg gttagtgaag cagtttgatg aaccgaaggt tgtagtcaca
      181 gataaagcac catcaattgc tagtgccttt aagaaactaa aagaatacgg attttatcaa
      241 gggacagaac atcgtaccat taaatacctg aataatttga ttgaacaaga ccatcgtcca
      301 gtaaagagac gcaataaatt ctatcgaagt ttacgcactg cctcacccac gattaaaggc
      361 atggaagcca tccgaggatt atataagaaa acccgaaaag aaggcactct cttcgggttt
      421 tcggtctgta ctgaaatcaa ggtattattg ggaatcccag attaaatcat agataccgta
      481 agggatttta ttctttattt aaaactttgc aacagaaccg tccgttgcgg gtaataccgt
      541 aacactgaag ggcacgcatt aaggcgtcat cttaatgctt tcctgtagga cactgacgga
      601 gattaccagt ctcccgagcc ttacaggggc ctttcttttt ttgtattttc aattatcaca
      661 atttatcgtc caaaagtgca gaacgaaaat caattcatca caagcatatc agacaattaa
      721 atttaaaaca actaaaaata tacaaaaaag tgtaattgaa tattagcagc actgcctgaa
      781 aagaagtaat atcaggcttt tcagcttggg gaaataagtg aattacctct cttcatgaat
      841 tattataact caaaaaagca catatgcaaa cactagttcc ctattttttt gaatgaaaaa
      901 taactaaaat atctaacgat tatgacttta aaacctacgt ttgcgattgt tttcataaac
      961 ttttaatcta aaaactccgg aataatagct atttatttgt aacatttgta ataaaattaa
     1021 aatcataaaa aagttctaat ttaaaattga tcatttttat agtgatcgct ttttatcaac
     1081 tactctagcc aattttccgt actaactgtt ggtaattcgt taaatgtttc tctttgttcg
     1141 tttgcaagct ttgccgcaat agctttttcg acaaaccaat tttttacata aacgaaaatt
     1201 agattctgca aattgtttac tttttcagtt ttttgttttt gataagcttt cagcaaagtc
     1261 atatataatc ctttgtcagc atcaattcca taagattcca gttcttcata aacaacaact
     1321 gtttgctcta attcttcagc ttttttcttt gcattatgaa tagtttggac ggttttttga
     1381 gcgtcttcgt aagtactaga aaatgttttt atcaagttta atacattttc tggtacaaat
     1441 gtatttatac tgtcctcttt catgatgtcg acgaagttat ctagtaaaac ttgattttgt
     1501 aactgatcag tttcagtgtc tattatgtgt ctattagtgt ctatagaatt agaatagaat
     1561 agattgtctg cgattttcgc aattacggtt gagtccttga tatgactgga ttttgccgta
     1621 tttgcgattt tcgcaattac ggtggtatca ttgatttctc gcggttttgc cgtatttgcg
     1681 attttcgcaa ttacggtggt atcagtattg tcttcctctt ccatgatttt aaagacgtct
     1741 tgattcgtta cttctggctt gcctaagtac aataaattag gcatggattc aatcttaccg
     1801 tcacgtttat ttactcttcc tctcttctgt ttcagaagac ctgctttttc taattccttt
     1861 ttgattttag atactttacc ttcgcggcat tgtaacaatt tcattaattc ttcaactgtg
     1921 aatatgaaaa atatattatc atcagaatct acccagttat ttttgactga ataatcaaat
     1981 cgatctttaa gtagcatata agcaattttt gcatcatttg atagatccat gtatttttcg
     2041 ttagtaaaaa ataccttagg catttgataa tatttttcac gataaatttc ttttgatttg
     2101 taataattaa aatcattcat agccaatcct ctttctgtag gatagcgttt tgttcaaaac
     2161 tatgttacaa tgaattaaac aaatatatct atccactata tatttttcta ctgctgataa
     2221 gtcgccaaac tttgttaaag cagtagtctt tttttatctt tttttagttt accttaaaaa
     2281 ataaattaaa gcaaaccgct ctatttcaag gtttaagctc atagtaacta tatcacgata
     2341 ttacaataat attatatcac gatatcgtaa tattacaata tgaagataag cgacttatca
     2401 gctttgatac tactgatttt tataaaaaaa atatcacgat attatgatat cgtgatattg
     2461 aatgtaaatt aaattttatt ttgtttcttt gctagtttat atccttcaat caagtattct
     2521 tcaggtatgg actttaatag cgcatcttct ataacgtctg gtttactttt gtctgattca
     2581 gcaatagtcg tccaatattt gagtgctgac attagaattg gatttatacg aatgctatct
     2641 gctgttctta cactacttcc ttgatctttt atgactttac ttatattttt cttggttaat
     2701 tcgatttctt ctacttcttt tgatttaaaa ttctttttat ttttatttcc tagtaatcta
     2761 cttactgcag cttgactgcg attatcttct gccatcaaaa tcaccaactt cttttaaata
     2821 ttcttctgta aatgctttat agtcatcttc agctgttgag ggtttattaa aattgattag
     2881 atctgatcca gcagcttgcg cttcgtcaac ggttattgtc ctacgaatat aagttttgaa
     2941 aatttttgca ccaatgactc tagaaatttc ggttgctgca gacgtcattt ctttagaaat
     3001 gttataacgc ttattatact tagtaaatag aactcctcgt acttcaagct tgtcgttcgt
     3061 atattctttt accgtttcaa tagtttcact taactttatt agtcctttta atgagaaata
     3121 cgcagcttca actggaatga taatttcatc agaaacagta aatgcgttga ctgtaagcat
     3181 tcctaaactt ggtggacaat caattataat ataatcatag ttgtccttga ttggttgaag
     3241 tactctttta agcctgtgtt cacttcctac ttgagtaaat tccctctctg cagaagaaag
     3301 ggctatgtcg gctggcaaaa catcaattcc tttatagtcc agaatagcat ctgtaatgtc
     3361 agcctcccct tttaatactt cataaattgt aggttggttt gttccatcta tatcgaaacc
     3421 tacattatct gtagcatttc cctgaggatc aatgtccact aaaagtacat ttttaccgta
     3481 aacaaatttc aaacatgctg ctaaattaac gctagtcgta gttttagaaa ctccaccttt
     3541 ttgattggct actgacaata ttcttgccat taaaatatcc cccattcatc ttatattata
     3601 atataattat atcataatat tgtgatattg caatatcgta tatgcattat attgtaatat
     3661 tgtgtgatta ttttgtttta aagatatata tcggttcgga tataaagatt attcatatgt
     3721 tattcgaaat taaaataatc actttttatt actttaaaat tttttattaa tgataagaaa
     3781 tatgttttgc gagcagcaac tagaataatt taaacattac taaactgtct atattgttac
     3841 taataattcc cttcttatag tgcattttct tagaaaaaaa caatattacg atatcatgat
     3901 attaagatat cgtaatatta tacaattgtg attgatatta tatatgtttt tttatttttt
     3961 tatcatgttt aatcttgaag gaatcatatt ctttttctaa agacaggagc ttttcataaa
     4021 catcagacat gtttaaacta gaggccaggc ttaaatcata aacgaatgaa gcaggtaatg
     4081 attttattct acgttgtcgg gttatccagc ttgcaaccgt tccttgtggg taggcatata
     4141 actcacaaaa ttgttctaca gtgattccta aattcttgat aatgtaaaca ttgatcgggt
     4201 gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc tactttcttt tattattgcg
     4261 tttgcaataa tagtttaaca cacgcattat tctagtatct actcctattt gtaaaaacaa
     4321 aaaaggattc atagagataa ctctatagaa tccgtaaaat accttatata tataacggga
     4381 agatataaaa cctaaaatta tgataacctg ctgcagcttc tacatagaaa gagaagcgct
     4441 caacaccaac ctcatttatc aaacgttggc cataacccca aatctcttct gatagctgat
     4501 caaaggttat atactttagc tcatattgct ggataatgtt cattctaatc gactccattt
     4561 attatcctgg taaaattcga tatgacgtat ttcgtcgtag tgaattttgt cttttccaac
     4621 gtagatcccc agctcatcac tacctaagat cttgccaatg atatcttctg gatagtatcc
     4681 ttcagcgttt cgttcttctt tttgaataga tactgatcta ttttttatga ctgcagcgtt
     4741 caatacttcc gcaatctctt cagtcgtcat ttgttctttt gctaaatttt gactggttcg
     4801 ttgttcattg tcggcatcaa tttcttcagt atgatcagat aaataaaacc ctgcccactt
     4861 taaacgtcca cgatcattgt aatcaatcat ttttaattcc cccatttccg cccgcatgac
     4921 caccaacaag tgtgcttcta gtaatgcttc tagcgccttc taacattgaa gaagcatgta
     4981 caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat aattttatct aaattttctt
     5041 cgtcaatttg cttttgtgga tcactaaata aatccagctg aagcgaatcg gtgtacacaa
     5101 gcttgctata ggttattcca acatgcctta cttcttgtcc ttcataatac ttatcaaaca
     5161 agaaaagaca ataattctgc aattcctttg tattatctgt gatagggatc ttcatctggt
     5221 gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat gtgtaaatgg acacaacctg
     5281 ttttacagtt atgctgccta atgcgtatcg ctacttgttc cgccatttct tttaccacca
     5341 gctcgatttc atttcttcta gtataatctc gtggtaacac ttgtgaattt ccatatgatt
     5401 tttcctttgt cggttctggt gggattgcta tatctgttcg atcgatccca ttagcatgga
     5461 agtacagctg taagccaatc acacctagtc tattcttaat cgtataagga ttccagttag
     5521 caagatctct aatgctcata atccccatct gattgagtct ttttttcatt cgagagccaa
     5581 ttccccaaaa gtccgtcatt tcaggaatat tccatacttt ttcaggaacg ttttcgtacg
     5641 tccattccgc aacaaatcct tcattatgct ttgcttcgtt gtctaaggct agtttagcca
     5701 aaaggggatt atctccgaca cctactgtag caatcaatcc tagctcttct ttaatattct
     5761 cctggatcat ttgagccagc ttctttctcc gttgacttcg tgttccttca gtcgtaaaaa
     5821 gattcagtga tcgggtcact tttaagattg attcatcgat cgagtaaatc agtagatctt
     5881 catcagccac atatcttctg aaaatattat ttacctgcat atttcgcttg atatataggt
     5941 tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg ttgtggtaag tcacgtggtc
     6001 tactcacatt tgtaatacca taccgctttt ttgcttcagg agaagaagct aatatcaatc
     6061 ctgaaccagt attatcagct cgactcataa caacaagttc tgttgttaat ggatctaaat
     6121 ttctttctat acattcgaca cttgcgtaaa agcttttcat atcgattaaa aagtaatcat
     6181 ttactggctc ttttgaataa tccatcataa ataacactcc attctttcac attatacaaa
     6241 catatgttcg atattgtaaa gaagaaatag ctaaatacat aaaaaaagaa gcataggtat
     6301 gctatcaccc tgcttcttct tttgatttgt atgatcaatt tattctatca ctttactaat
     6361 taatttgaaa atctaataaa aactctcact ctaaaagagt gagagactgc aaattagagt
     6421 acggactttt ttggagttcc gttctttaat attaacaaat gaataaaatc cttacaacat
     6481 ttgagaataa gttatcagta gacacttaca aaaaattctc atactatttc ctccatcatt
     6541 agtttttgtc tatataaggt ttaccctaaa tagacactgg tataacattg aaaaatatgg
     6601 cttcctcgtg tatgattagg gtgtatttta aatagttgca gaaagtaggt aaaagttttg
     6661 agagttgcat acatacgtgt ctcatcgatc gatcaaaatg aacaacgcca gattgaagaa
     6721 atgaaaaaat tcggtgcaga gagaattttc atcgagaaac aatctggcgc aacgattact
     6781 catcgacctg ttttccaaga agcattagat tacgtccgag atcaagatat ttttattgtg
     6841 gaagctattg atcgacttgg aagaaattat gacgagatca tcgcttcagt caattacttg
     6901 aagaagaaaa atgtgaagct gatcattact agtttaccga tcatggctga agcaattggc
     6961 aatcctctcc tggataaatt cattaaggat ctgatcatcc aaattttggc catgatcgct
     7021 gaacaagaac ggactgaatc gaaacgtcgc caggcacaag gaattaagat cgcaaaagca
     7081 aacggcgtct acaaagggcg gcccaaatta tacagtgcag acgcaaaaga tccccaacgt
     7141 cgccttgttt acaaaagcat cgttgaagat cttaaaaatg gtgttgcgat tgctaagatt
     7201 gctaaagact acaatgttac gagacaaacc gtgtatagaa taaaaaaaga cagcatggtc
     7261 aatgacaaat agttgatcat gctgctttta ttatcttctc atctttaatt tcctacatat
     7321 aattactatc tataaaatta agtcttactc agaacagata aagcataaaa ttccaacaaa
     7381 ccatttttta cttaactaaa taaagagtct gttaatataa tcttcactta tttacgttaa
     7441 tcccctactg tatttgttgg ttccttatct atattttcta caaaatcctt attatcttgt
     7501 gatttagctt cgtataattc tttgtatgtc gacacattgg ttatactaat tcctatagct
     7561 agttcttgag actctacttt tctctctagc tcctttgaca aagtgatttg agatttattt
     7621 ggttccgttg gagtcaattc tatagattct tcataaaaat ctctatacat aaagctatcc
     7681 ctgaataatt catagcttta attctttggc acattttatt gaaatatgta tcactaatat
     7741 ccctatcaac tgattttgac taaaaagtta cttcaaacat tcactgttca aaaatattga
     7801 ggaataaatt ttgggtgtaa ggcattttga gcatacttct ccctggataa cttcccagta
     7861 aaaaaatcgt tagattgttg gattagatcc attgactggc tcgcctcagg cgagcaaaga
     7921 ccctgtagaa ttcattctga tacacatgat aactagataa tttgagatag actcgtctac
     7981 ccgtttggac taatttccca gcaactttca gaaacttcaa tcgaatagaa tgaatggtca
     8041 ttcccttttg gacttcttca aagccaatcg ttcttaagaa gttgactaag ttgtaagcta
     8101 tcaagctgag catcattcgg acatgattct ccaaaaagcg aggactgtct gttttgtcaa
     8161 agtaaaagcc agctttcgct tctttaatga agttctccat tgtgccacgt ttggcataga
     8221 tagaaaagat ggtatcagga gaaacatttt ctgatagatt cgtcacgata aattcatgtc
     8281 gaaagagtag ttcgcccgct tcacgtgttg agcgtataca cacgcgacga ctttgtgacc
     8341 aggattgtgc ttgataagtg gtcgagaagt actgaacttc tcgttcttcc cacttttgat
     8401 tatcgccata aagtactgat ttctcagcta tttgacctag tctacgatta ttcttcagtc
     8461 gaataacata atgactcttt tttgattcac acgaatcata cacaccaggt gtagcgaacc
     8521 cgctgtctcc acgaaccaag atgtcagtgt ttggtagaga gtgattatag tgctctaata
     8581 aaggtgtgag aaactccttt acgccttttg atgtatattg atttcctgaa cgtagttcag
     8641 cttttaagaa gtcaccagtc aatccgtcaa aagctaccaa tggatggtaa ccataggttt
     8701 ggtagtgtgt attataattc gtttgttctt ggtgaccaaa tgtatctgaa tgggtcgaat
     8761 ctaaatcaat gattaattcc gtatcattac ggatgaggcg tgctttatcg ataagttctt
     8821 ggttcaaagc ttgaagttca tggatattct cctcagatag tcgatctaaa aatcgggaaa
     8881 gtgaagattg agaagcgagt tctttcctat ctaaaacagc tttaaacaca ggatcttgtc
     8941 tcaggagatt agctgcagaa tcagctgagt aaccagcaat taattgcata atgaactgct
     9001 caagtatgga taagttgtca tgagtaaaat acgctcgttc gtcttcaatg tgaatgtgtt
     9061 gcttagccag ttcagaaaaa ccaaagatgt tcatcagctc tttcactagg acgagacccg
     9121 aatcactcga taattgacca cctgtatgag aaatagtgat atttgaattg aattttactt
     9181 ggtttttgtg taagctagtc atgaaaagaa ctcctttctt ttggttggtt tagtcacttt
     9241 aaccatagca gaaaggggtt cttttttcat cacttaacgg gtgaagatga aaaagtaatt
     9301 gtaagctgcc gtgaggcagt ttcacatggt tttaattaaa agtatgaatt attcaggcta
     9361 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     9421 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     9481 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     9541 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     9601 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     9661 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     9721 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     9781 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     9841 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     9901 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     9961 aaatcagcta atttttcttt agaataaaaa aaaacattta tatcagaatc aataaattct
    10021 atttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
    10081 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
    10141 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
    10201 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
    10261 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
    10321 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
    10381 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
    10441 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
    10501 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
    10561 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
    10621 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
    10681 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
    10741 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
    10801 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
    10861 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
    10921 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
    10981 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
    11041 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
    11101 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
    11161 aaatgattaa tattctaact tcttataatt aaaagtgcta tcacttttag tttttaggat
    11221 aaaaagtgac gtcacttttt gagtgctggt acctcaaatc ctgctttttc ttgggcaatc
    11281 aataaatcta acgcaataca cataatcctg gattcacttt tgccgtactt ctgctttagt
    11341 tgctgcaatt ctttttgtct cttttcatcc aacgtaacgg gtaaccgttt atttcctatt
    11401 gcaattggca ttagtactct ctcctttcat cgaaataacc ctatgaaaaa tatggctgca
    11461 atcatcttga atagtagact cacaagggct aatacaaagc gccaaaagat caaacaaaat
    11521 accagtattg ccaaaaatac tacgaagttt aaatgcacag ctgcagcatg gcgcataagt
    11581 atttcgccaa caaataggac taacaataga ggaatgattt tattcatatg gatctccttc
    11641 tttctcttag gttatcgggt gttgcccgct tatttcgcac cacctaagcg gcgggggcgg
    11701 ttggagcgat tggtgcgaaa taagaaccat tcccttagtt actaggtttt ttgtccttat
    11761 ttcacacatt ctcacttttc ggtataaatg attattgcct ttttttcctt tcaaatcgtt
    11821 gatatggcat tcgtttacgg tcaagttcat tttggatttc ttctgtatcg tcgtaagtat
    11881 aggtgtctaa aaaatcagtt aaagtcgt
//
LOCUS       DANJMI010000078        11781 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000078 DANJMI010000000
VERSION     DANJMI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.078"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            507..1070
                     /locus_tag="PWU81_002677"
     CDS             507..1070
                     /locus_tag="PWU81_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111314.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1131..1496)
                     /locus_tag="PWU81_002678"
     CDS             complement(1131..1496)
                     /locus_tag="PWU81_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111315.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1572..1817)
                     /locus_tag="PWU81_002679"
     CDS             complement(1572..1817)
                     /locus_tag="PWU81_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1111316.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1887..2141)
                     /locus_tag="PWU81_002680"
     CDS             complement(1887..2141)
                     /locus_tag="PWU81_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111317.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(2305..2628)
                     /locus_tag="PWU81_002681"
     CDS             complement(2305..2628)
                     /locus_tag="PWU81_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111318.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2815..2888
                     /locus_tag="PWU81_002682"
     tRNA            2815..2888
                     /locus_tag="PWU81_002682"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2849..2851,aa:Ile,seq:cat)
     gene            complement(3273..3770)
                     /locus_tag="PWU81_002683"
     CDS             complement(3273..3770)
                     /locus_tag="PWU81_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL1111319.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3810..4619)
                     /locus_tag="PWU81_002684"
     CDS             complement(3810..4619)
                     /locus_tag="PWU81_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1111320.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4953..5810
                     /locus_tag="PWU81_002685"
     CDS             4953..5810
                     /locus_tag="PWU81_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL1111321.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5803..6705
                     /locus_tag="PWU81_002686"
     CDS             5803..6705
                     /locus_tag="PWU81_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL1111322.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6738..7088
                     /locus_tag="PWU81_002687"
     CDS             6738..7088
                     /locus_tag="PWU81_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL1111323.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7137..8060
                     /locus_tag="PWU81_002688"
     CDS             7137..8060
                     /locus_tag="PWU81_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL1111324.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8138..9031
                     /locus_tag="PWU81_002689"
     CDS             8138..9031
                     /locus_tag="PWU81_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1111325.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9069..9632
                     /gene="amaP"
                     /locus_tag="PWU81_002690"
     CDS             9069..9632
                     /gene="amaP"
                     /locus_tag="PWU81_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL1111326.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9646..9834
                     /locus_tag="PWU81_002691"
     CDS             9646..9834
                     /locus_tag="PWU81_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL1111327.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9847..10749
                     /locus_tag="PWU81_002692"
     CDS             9847..10749
                     /locus_tag="PWU81_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL1111328.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10781..11023
                     /locus_tag="PWU81_002693"
     CDS             10781..11023
                     /locus_tag="PWU81_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL1111329.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(11279..11650)
                     /locus_tag="PWU81_002694"
     CDS             complement(11279..11650)
                     /locus_tag="PWU81_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111330.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4175 a         1985 c         2233 g         3388 t
ORIGIN      
        1 cattggtcgt tgaatgggct gaatagtagt gtataaaaca attttgtgga gcgtgaagct
       61 tttatggtag aggaaaatac gaataaagtg atgttgataa aaagattcct atcttgaata
      121 tgaatcgaga tgtcttgaaa atgaatgagt ttgaccaatc attatcctat cgaaatttac
      181 agtttcgttc gattggtctg tcaattaatg aaaaagtaaa aaagaatatg ttctaggaag
      241 agggcggcac atctaaattt ttaagtaacg gacagttcaa atatcaagca ttgaaagagg
      301 ctatcctact gtttgaagga atacgaatat ataaaaacga caaagaggaa aaagtagttc
      361 attaaatccc ttcaagtgat gaaagaatga gatctaataa aacctgatct ttcatctgtt
      421 tttttaataa cgtaataatt tttttatttt ttgtgctttc gccgttattg tagagtgatt
      481 tagcattctt atttttaaaa ataactgtgt tatatattaa aaaaggcata atagttataa
      541 gcttttttaa agaatattta gaaattatta attttcgtct gaaaacaaaa aaagaaagcg
      601 ttattatttt agtctatttt caggggggga accttatgat aaaaaaacta tttttactat
      661 caatattaac gctagcaatg gcaggttgtt ctgactctgg cttagcaact gaactgatca
      721 caaaatcaac tgaagccacg acagaggagt taaaatgtgg aaacgatcac agtgactaca
      781 gaggcttaaa tcgacgatgc atgggaatat taggcagaag caattgtaag catcgatgta
      841 gtaatcgaag aaggtgtaga agaagcaaca gaaaaatcaa aagaaagatc aaaagaagca
      901 atctgtgctg tgcatcgtat acagacgtcg aagatgcggt acttttaaac cacgtcagga
      961 gatgttcatt tatgtctagg gaagttggcg atgaaccaaa actagagtac gcttttagtt
     1021 ttgcacgtga ggatgaagat aaccctaaaa cttacgacat tttagcttga tctaagatgg
     1081 ttaaagcttt ttaatgaagt aagaggagat agaaggcttt tctagttata ttaggtaaga
     1141 taaattcctt tttgctccaa atttgctaaa cattctgcta gataaacttc atcgtgttcc
     1201 ggataaatcg gcacgttgcg ctctacagta ggtaaatcaa aaaatgcttc gcatgatttg
     1261 ccacgataca taggattttt ccactctggt gctgggctgt aaccacgttt ttccatttca
     1321 tccatgacaa gctgatgata ttggaataat ctataaggag aatagtgaaa aacataatcc
     1381 acagtagcat gtttctttcc ccagccattt cctcttaatg cacagcattc ccgatgttgt
     1441 cctaaaagct gttgacgagg aagctttgaa atcaattttt catgccataa tcgcatagaa
     1501 atgtctcctt tatatataga ttgttaacct tagacgttaa atgaaaaaac cagcaccaag
     1561 gctggaaaaa atcacttcag tttgcttgct gtttctgagc gaccaattag gtaatcaacg
     1621 gaaatatcgt aaaagtcagc gatatagatt aattcaatga tgcttggaat ttcttctcct
     1681 tcttcgtact tacgatattt gaataaagga atacttaacg cgctagaaac agctacttga
     1741 gataaagatc ggccagttct taagaaattc agacgacaac gtaaagtatc cagtaaaata
     1801 gcttcttcga tactcattta atcacctttc attatagtag aatagttata atgactatag
     1861 aaagctacac atgcaatcaa tagtactcaa tgttttttat ttaaacgtat caaataaaga
     1921 atgacgatca ataaagacaa aaccagtcct gataattctt ctataaaaac actatcgggt
     1981 tcaaagtagt tgtaattaaa aactttccac ataaagtaga tagccggaag agtcaaaata
     2041 gctaaataaa accgttcttt gttccaattc ataagataaa caatacttgc gaccagtgcg
     2101 accgcaggca cccaaaacaa aactctcacc aacgtaatca tgtaacggac tcctcaacga
     2161 ttcatataat aaaagataac atgttattgc atacatcaca aatgaaatgc taggaaaata
     2221 aaagaagaat agatcaatga aaaatgaaaa agcagaacag ttttctgctg ataagcagga
     2281 actgttccgc tgaatgtgtc ttatctatcg agaaaaaatc aatggattga tatctaagta
     2341 attcaataca tataaaaaga ggacgatcgc attcactaaa aaggaaagaa tgatgatccc
     2401 atagcgaatg gaaacatatt taacaaatac ttttgtttga atacgggcat atctttttcc
     2461 tttatagctg agcatcatga caagaagcaa aagggtaatc acaccttgaa aaatcattcc
     2521 aataaacaga attcctgagg tttgataatt taattggaga aaataagctg ctgccgcaac
     2581 taaatcgaat aaaatcgcaa taaatggcaa aataatcact cctaacattg agaataaata
     2641 aaagaataac attttttcta aaataagcaa ataaaaatta aagtaacaaa atagttatga
     2701 cacaagtaaa agaaataaga ataacaatat tattttactt atattttgaa acttgtgaga
     2761 gtttgtcatt cgtgctatag ttgaaaagta acaaaaataa attttattta tgatgtacct
     2821 ttagctcagt tggttagagc agacggctca taaccgtccg gtcgtaggtt cgagtcctac
     2881 aaggtacata agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaagta
     2941 aaaacagctt cttatgtttt tacttggctg ttcttattac tgcagatgca agcttttgaa
     3001 taccgtttaa ggaagaggtt gtgaaatcag cgttttgacc tgagtattag cttaacaaac
     3061 ggaaaaatag ttcctcatat tttgccgttt gttaagctaa taccgagggt cagctgattg
     3121 aacaccattt aagaaagagg tcgtgagcct gccgtttcac tctgtgtcat aaaaaagcca
     3181 atctactttg atagaaaaag cacgtaggac taaaacagtt ctgcgtgctt tttttattgt
     3241 gtgttaaata tcgtaaatga agatagatat ttttatgatt cgacgataga gacgttgaaa
     3301 tctttcaaca gctctaaagc ggattctccg tgttttctac tggagactaa cattggatca
     3361 acaaaaaagt ctgcttcagg aaaagcactc tgcaggccta tcagattaga gagtacacac
     3421 aagtttgttt caatcccgac tacttcgatc tctttttgtt cttctttgct ttcaaataaa
     3481 gtttcctgga tttcagctaa agtttcaggt ggaatcgtaa agtaatactt tttaatcact
     3541 cgttcatttt cttgtggaga aagaagaggg atcaattgca aatcttctcg ttcttcttct
     3601 ttgtccttca gttcaactgg tatatcccgt gtgaagagaa tatattcatt attttggcgt
     3661 gctttagcta atctttgagc aatacgttct gccaattgat ctgactctgg aatataaaat
     3721 tcgctgtttg gatcaagaat atgattttgc atatcaataa taactaacat tttctttcct
     3781 cctacattca tatcgtcatt ttattctttt taatagaagt tttttaaaat ataagcaaca
     3841 ccagcttctt cattcgtcag tgttacttgg tttgcaattt cttttacttg tcgctgtgca
     3901 ttccccatcg caatactcaa cccgctgtaa ttcaacatag caatgtcgtt ttcttgatcg
     3961 ccaaaagcca tgatgtcact cgattcgatt ccaaggtaat ccgccagaaa aacaagtgaa
     4021 tgagctttag tgatacctgt aggcgtgatt tctaagaaat gcggatcaga gaatactgat
     4081 tggataccat ctgcttgcag atctctttgt agcttctgta gtttttcttc actcttatct
     4141 gttaatgcta atttaagata gctaagagaa ggatcttcca acatatttaa aatttgatca
     4201 aaagaatggt tctgtaaatt gaaatgttca taataagggt catctttgac tagatttgat
     4261 tcatcgaatg tcagctgatc atcttgggtt tctcggctag caatgatgga tattttcgct
     4321 tcttgagcat agaagaaggc ttttctcagt gtatcgctat ctatttttga acgtctgaga
     4381 actttcgcca tttttccatc cttcatttca gcaatcagtg aaccattgga agaaatcaaa
     4441 tagccaccga ttcctaattc ggaaaaaata tctttagcag aaatgctgtt ccttccggta
     4501 gcgacggtta cgattccgcc attttcatca aatttccgaa gtgcttggat attttcttct
     4561 ggtatctttc catttcgatc aagcaaggta ccgtctaaat caatcgctgc tagtttcatt
     4621 ttatcttcct actttctttt tacatatcta ttttcctgtt accttaagat taaatgagaa
     4681 tttaaaaaaa tgaaactaac ttgctttgtc attatgaatt ttttcaaaaa acattcttaa
     4741 gaaaaaggtg gctatatata gtagtttttg gtcaatacct ttgaataaaa agccatgaaa
     4801 aacgaatagt tagcgcttac cgatgcgttt tgtgataaaa aaattttaaa atattcaatg
     4861 aaaacaaagc atatattttg ctttaaaacc aaatgaggaa tattctagat gtagaacagg
     4921 ttagaaaaag attagaaaaa ggagtgattt tgatggagaa gagatacggc gtagttcctg
     4981 tggtaactgc ttcagacgaa aattatgctc cttacttgag cgtgatgatc gcaactgcat
     5041 tagaaaattg caataaaaca agacgcataa aattttatgt gattgacgat ggtctatcag
     5101 aatatagtaa agaaggattg gaagaaacgg taaataaata ttcaagtaat gcaagtatcc
     5161 aattcctgac agtggaaaaa gatatctacg aagacttcct agtcagtgac catatcacaa
     5221 ctaccgctta cttaagaatt tctttaccaa acttattagc taaagaggat tataagaaag
     5281 tcctttactt agattcagat gtgttagtac tagacgatat cgtaaaacta tatgatgagc
     5341 cgttaaatgg aaaaacaatc ggcgctatta tcgatccagg ccaagtgaaa gctttggaaa
     5401 gattaggaat cgattccgat gacttgtact tcaattcagg agtgatggtg attgacattg
     5461 atcaatggaa taaaaaagaa attacagaaa aaactatcca ttacctcagt gaaaatggcg
     5521 atcgaatcat ctatcacgac caagatgctt tgaatgctgt actttatgaa gattgggaac
     5581 aacttcatcc aaaatggaac atgcagactt cgctgatttt tgaacgtcat ccagcaccaa
     5641 atgaaaaata tgaacgtcag tataaagaag gaaatgaaaa accttcgatc gttcacttta
     5701 caggacatga taaaccttgg aatacgttga aagatcatcc gtataccaat ttatacttaa
     5761 aaaaattagc acacagtact ctaacgaaag taggcgagat caatgaataa aaaagaaata
     5821 gcagtagtcg caagttgtaa cacaaaattc gtaccacatt tagctgcatt atttgtctca
     5881 gtattagata attgcaaccc gtctaaattt gttcggttct atgtgattga cgatgatatc
     5941 gattttgaaa gtaaacagct tttacgcttc tcagtgaaaa acgcacgaat gaactcagat
     6001 gtggaattct tgaaaatcaa taaagaattc tttacaaatg tggtaatcag cgatcgtatt
     6061 ccagaaacag cttattatcg tattgcgatt ccggaattgt tccgcggcac agaagttgaa
     6121 cgtatcttat atatggattg cgacatgatc gctttacaag atatctctaa attatggcgt
     6181 cttgacttcg gtgattcaat cgtagcagct gttgaagacg ctgggttcca tcaacgactg
     6241 gaaaaaatga aaatttcagc taaatcaatg cgttatttca actcaggttt gatgctgatc
     6301 aatgtgaaaa aatggttaga tgaaaatatt acgcaaaaag tattagattt catcgaacat
     6361 aatccagaaa aattacgatt ccatgaccaa gatgcattaa atgctatctt acatgaccgt
     6421 tggttaccac ttcatccgcg ttggaatgca caaggctata tcatggcaaa agcaaaaaaa
     6481 catccaacag cagcaggtga acgtgaatac gaagaaactc gcaacaatcc ttacattatc
     6541 catttttcag gacatgtgaa accgtggagc aaggattttg aagggccaac gaagaaatat
     6601 tacgaaaaat atgcagggat gacggctttc cgttgtgtag ctaaattccc taaatatcca
     6661 aaatatgcaa aagttcaacc aaaaacagat gtagttgtta aataatagaa gaccacaact
     6721 gaagaggggg agtaggaatg gaaattacaa tgtctaaaaa tgcagtagag acgttaattg
     6781 aaaaggttgg ggaaaataca aaaatcgcac tcgcgcttat caatgattcc gatccatttc
     6841 taagggacaa aggtgctttt gcaaaaggca gcttttttca aatcattcct tttgtttcgg
     6901 aatttggaga atatgcgact aaaatcgagc atccattatt ggacatctat acatctaaat
     6961 tagagcaaaa ttatttcggt aaacgattga acatggactt caacaaacag cttgacagct
     7021 tttcattaga aaatgaaatc gcagtactcg attataatat caaattaaag aactgcttct
     7081 tcagctaata gtttcgagaa agtccaaaaa ggaggtatac attgagatac tactttgtgg
     7141 atgaagaaaa gaatgtaata gaaacagatg aaaaaaaata taattggctc gtacttgata
     7201 gtgcaaagcc cgaagagatt gagcaagcaa tcgaagaatt cgagcttcct gatgatattt
     7261 tcgtaggaac cgaatatccg gaagaagttt ctcggatgga gcgcctttat gagacgaaac
     7321 tagagcatcc attttctttg gtagtgatta atctagatca gtcagataat cgtatcgaaa
     7381 aaaaattaac gccaatatct tttgtacttt ccgataatct cttgattact tgtatcgata
     7441 gaaaaaccga atttgtggat cggctgctga caaagcatgg aaatcattta aactcgtttg
     7501 aaaagatcat cacgtataca ttattagata tttacaccca ttacgtgaaa gaactaagag
     7561 agatgaaaaa gcgaattgac gctttggata aagcagctcg taaaacaacg gaaaatgaag
     7621 aactgtataa acaggcagat cttgaaagag acatcgtata tatcgatcat accttaagag
     7681 atcaacggga aacaatggat caattatgga attctgaaac tttcatcaaa cgtttagacg
     7741 atgaacagct actctatgat gtccatttga gacagcgaca aacagaaaaa atgatcgaga
     7801 tctatcgaga tttattagag agtattggtg atctattcaa cgggatgatg gacaataatc
     7861 taaatcatat catgaaatat ctagattcag ctgcattaat catttccgtt ccggcgttgt
     7921 tcgcagggat ttggggaatg aatacaggag gattaccggg taaaagttct gctctaggct
     7981 ttatactcgt agtctttggc tctttgatag cagcagtagt tttaggttac catttatacc
     8041 ggaaagatta tacaaattaa agaatctatt taaaacaggt atcaaacaaa gaaatttgtt
     8101 ttggcaatac aatttaaaaa ctaggaggaa tacagcaatg acagaaccaa aattaatcga
     8161 cccgcgaaac ttgtaccata cggagaaatt tcctaaacaa gatcaggata caccagcttt
     8221 acagcagaat atgattcctg ttcctgattg tggagaagaa tcctatgaag gcaataatca
     8281 actagaaaac cgccgtgtac taatcactgg tggagattca gggatcggtc gtgcagcagc
     8341 gattgcattt gctagagaag gggcagatat cgctcttcaa ttcttcccag gagaagaaaa
     8401 agatgcagag gaagtagcaa aatacgtgaa agatgcagga cgtaagattg tcttactacc
     8461 ttttgatctt cgtgacgaac aagcacctaa agaaatcata gataaagccg tgagtgaact
     8521 tggcgggcta gatacgatgg tactaaacgc aggacagcaa atttcttgtc agtctattgc
     8581 tgagttacca cttgagcaag tcaaagacac atttatgata aacatcatcg ctatgtttgc
     8641 tttagtgaaa gaagctgttc cgcatatccc agcaggagga gcaatcgtaa caacaacttc
     8701 tgttcaagca ttcaatccaa gcgaacattt attagattac gcagcaacaa aagcttcaat
     8761 cgctaacttt acgatcggtt tagcaaaaca gcttgcacct aaaggaatcc gtgtaaatgg
     8821 tgtagcacct ggtccaattt ggacaccatt acaactagac gaaggacaac cttctgattc
     8881 gatccctgaa tttggtcagc aaagtttact agaacgtgca ggtcagccag gagaattagc
     8941 accagtttat gttttcttag catcgaataa agcaagttat gttacagccc aaatttacgg
     9001 agtaactggt ggagaagcga tcaacttata agaagttgat cgtttcaccc ctatgaggag
     9061 gtgcgaaaat gaataaaggt gtaaaaacga tcggagtgat cggaacatta cttttgctat
     9121 ctgtgctttt gtttgcgata ctattaagtg caacctatcc attgcctttt cagctagaga
     9181 agtttcgatt cttttccatc actaattcat acgtgcaaca atatgtattt tggcttgccg
     9241 ttgcatttgc tattttgaca attatcacgt tacttgtttt cctgttttat ccaaaaacaa
     9301 tcggaaactt tctactgaaa catggtgatg gggagttgac gttggataaa aaagcaattg
     9361 aaggaatggt tcgttctcac ttgcatgaag atgaattcat ccattcacca aaagtaaatg
     9421 tcaaagcaac gaaaaacaag atcgccgtca atgtaaaagg ggagttgaaa cgaacttcag
     9481 cactcattgg taaaacagga acattgatga aagaaatcga aaatgaagta caacacatat
     9541 tgggaacaaa agagccagtg aaagtatcgg tgaaatacac aggctttgaa gaaccagcta
     9601 aacgaagcaa tgaccgcaaa cgagtagaat agggggggac caaaaatgaa agaactattt
     9661 tcgacataca agctccctat catctttggg gtacttggat tgatattagc cgttttatgg
     9721 atcaccgtgg gctttttcaa gaccttattg atcttgatca tggcagcgat cggcgtcgct
     9781 atcggttttt atctgaaaca aacgaaaatc ttagatcact attttaaatc ttaggaggaa
     9841 acatccatga ataacaataa caaaaataat aacaataaaa tgaacaacca aaaaccagtg
     9901 tctgcacatc cagacccaac gacaccaaac caatcaaatg gtgtgaacga tgaaaagaac
     9961 aaaacagcac cagtatctgc acatccagat ccagtagtcc ctaacgttca acaaggagta
    10021 gaagagaaga aacatgatgc agaaaagcat gcacctcatc atccaggaac tccagaatct
    10081 aaagttccag aacaacatga tgcaatcgat ccttctaaac ctgtggaaga gcaaaaacat
    10141 ccttctgacc atgcgaaagc acaagaaaaa cttagttctg accacacaaa cggacaagga
    10201 aaacttggtt ctgatcatac aaataaccaa ggaaagcatg attctgaaca ggaagattca
    10261 atgcgcggtg agctgaatta tgaagataaa gttgtgcaaa aaatcatcgg aatcgcaatt
    10321 gaacaagtag acggtttatt atctgcaaac ggtggtttct tctctaatgt ggcaggaaaa
    10381 cttgtgaata cagataatgt aacggctggg atcgaaacag aagtcggtaa aaaacaagta
    10441 gcagttgata tggatgtcat tgtggaatat ggcaaagata tcgaaaaaat ctttgaagaa
    10501 atgcaagaag taatcggcaa agaagtaaaa aatatgacac atttagaagt gatcgaagtc
    10561 aacgctaacg tagtggacat caaaacaaaa gaagaatttg aaaaagatca agaaacggtg
    10621 caagacaaag taactgacgc agcgaaaaaa accggtgaat ttgcttcgaa tcaaacagat
    10681 aaagtaaaat cagcagctgg aagcggcgca caaaaagtaa aagaaaatac agaaccacgg
    10741 gttcagtaat aaggaagtaa aacattagga ggaacgaaac atgggattta tttggtcact
    10801 tatcgtcgga ggaattatcg gagcaattgc aggagcaatc acgaataaag gttcttcgat
    10861 ggggattatt gcaaatgtcg tagcaggttt agttggatca gcacttggtc aagcattatt
    10921 aggttcttgg ggaccaagta ttgcaggaat ggcaatcgtg ccatcattga ttggtgccat
    10981 cattttagta ctaatcgtat cattctttgc tcgaaaactg taaaaagact tttaaaaaga
    11041 ggtcgtgaaa aagctgttct tcatcaagta ataagaacag ccaaacaaaa atagcttctc
    11101 atatttttca tttggttggg catattatcg aagatgcaag cttttgaaca ctgtttatac
    11161 ggaataagag gctgtgacaa gacttttgtc acagcctctt atttatgcgg gcaaattccc
    11221 aattagattt ttaaaagttt ttccttaaat gtgtctttat tgatacagag aagcttcttt
    11281 aataaaacaa tgcctcccta aattttcttg agataggaaa ggtgaacata caaaataata
    11341 cgaccacttt gaaaacccag atgatccaca aatggatcgt atgatactga tgaagtaaaa
    11401 agcaatggat cagaaactca aagagattga aaaagacaag gaagaaataa tatctctttt
    11461 ttactcttgt ctgttttagc caataatagc tcgtaaagta atctagacaa acaaatagaa
    11521 gtaaacaaag tttcgtaaga gcagcaatcg tatcatgttg ctctgctaga cgattgacga
    11581 gcaggaaaaa tgttggtaat ggaatataga tcacgagtaa tgaaagaaaa aagtaaacaa
    11641 taaatggcat actattcctc ctaattgtgt gagttcgccc tcattttaaa ataacgatat
    11701 ttgtttgtga agctttttcg tgtcgtaaca aatgttatct aatgtatttt taaataaata
    11761 atcattatta tcaaaataat g
//
LOCUS       DANJMI010000079        11755 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000079 DANJMI010000000
VERSION     DANJMI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.079"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..177
                     /locus_tag="PWU81_002695"
     CDS             <1..177
                     /locus_tag="PWU81_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1111331.1"
                     /translation="YLLGMALAYSVGSLIKVNVSLDLFTILLAVGVSSVIGLVFSVMP
                     ASEAAKKDLIDILR"
     gene            578..1183
                     /locus_tag="PWU81_002696"
     CDS             578..1183
                     /locus_tag="PWU81_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HDL1111332.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1199..1771
                     /locus_tag="PWU81_002697"
     CDS             1199..1771
                     /locus_tag="PWU81_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL1111333.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2023..2280)
                     /locus_tag="PWU81_002698"
     CDS             complement(2023..2280)
                     /locus_tag="PWU81_002698"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111334.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2406..3221)
                     /locus_tag="PWU81_002699"
     CDS             complement(2406..3221)
                     /locus_tag="PWU81_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111335.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            3738..5063
                     /locus_tag="PWU81_002700"
     CDS             3738..5063
                     /locus_tag="PWU81_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL1111336.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5056..5406
                     /locus_tag="PWU81_002701"
     CDS             5056..5406
                     /locus_tag="PWU81_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111337.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5403..5573
                     /locus_tag="PWU81_002702"
     CDS             5403..5573
                     /locus_tag="PWU81_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111338.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(6477..6752)
                     /locus_tag="PWU81_002703"
     CDS             complement(6477..6752)
                     /locus_tag="PWU81_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HDL1111339.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(6724..7677)
                     /locus_tag="PWU81_002704"
     CDS             complement(6724..7677)
                     /locus_tag="PWU81_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL1111340.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8289..9782
                     /locus_tag="PWU81_002705"
     CDS             8289..9782
                     /locus_tag="PWU81_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL1111341.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            9917..10213
                     /locus_tag="PWU81_002706"
     CDS             9917..10213
                     /locus_tag="PWU81_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL1111342.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10314..11165
                     /locus_tag="PWU81_002707"
     CDS             10314..11165
                     /locus_tag="PWU81_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111343.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11186..11469
                     /locus_tag="PWU81_002708"
                     /pseudo
     CDS             11186..11469
                     /locus_tag="PWU81_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4041 a         1811 c         2179 g         3724 t
ORIGIN      
        1 tatttattag gaatggctct tgcttatagt gttggttctt tgataaaggt aaacgtgtca
       61 cttgatctat ttactatttt actagcggtt ggggtatctt cggttattgg actggtcttt
      121 tctgtaatgc ccgcttcaga ggctgcaaaa aaagatttaa ttgatattct tagataaaat
      181 cagaatgaca agaatattta cgttctagat tctaaaataa aacacaaaga gaccaaccag
      241 gcaccctatt gtttctttgt agtatagaga tattctttgg cttgtaagag atcaatagag
      301 ttaggtgacc ggagttcgaa ataaaggaaa tttttgattg tataggagaa ttctttatcc
      361 ctaatattta gaattttgag tatgccatgt tgatgtttta gattctctat aaatatagag
      421 gtgtatcaaa ggtcaactag actgtgcaaa atagagacac ttgaaaaatc tatcgggggt
      481 cagaaactta atagtttttg acacataaac aaaatcgcga tataatgggg ttgagagtgt
      541 ttcataaact tatgtcataa agttaaggag atttcgcatg attttagaga ataaattggg
      601 attaaccaat caagttgagt tggctaaggt tgaagaaaaa ctcagtaaac aaaaagcgaa
      661 agaattgtac gattgtggaa aaataaatga aatcgaagtt ggtacattta aaggtttatc
      721 tgaaattcat gaatttctat tttcggatat ttacgatttt gccggaaaaa tccgatcggt
      781 caatattgca aaaggaaatt ttcgatttgc accagttatg tatttagaac attcgttaca
      841 gcatattgat cagatgccac aaacttcttt tgaagagatt atcaagaaat atgtggagat
      901 gaatatcgct cacccatttc gagaaggaaa tggccgaagt actagaattt ggctggatct
      961 cattttaaag gaagaattgc aaaaagttgt agattggaac ttaatcgata aagcagacta
     1021 tctttctgca atggagcgaa gtcccataaa cgatttggaa atacgctatc taatttccaa
     1081 tgctctgact gataaaattg acgatcgcga gttatatatg aaaggaatcg atgtcagcta
     1141 tttctatgaa gggtattctg agtatacgat agatgatttg taaaagtagg tggaccaaat
     1201 gacgaaatat ggatatgcac gtgtctcaac cgcttctcaa agtacaaaag aacaaattca
     1261 acagttgctt gagaacggcg tagagaagaa gaatattttt tcagaaaaat ttacgggtac
     1321 aaaacgggat cggccaaaat tcaatcagct gttggctggt ctgaagcctg gagatgaaat
     1381 agttgttacg aagcttgatc gatttgctcg taacacgaga gaagcattag agatcattga
     1441 acctttactc gatgataacg taacgatcaa ggttttgaat ctaggaacga ttgaaaatac
     1501 gtccatgggg cgaatggtga cacggacgtt gttgagtgta gcagaaatgg aacgtgatat
     1561 gattgtggaa cgtacccaag aaggaaaaat gtttgcaaga aagaataatc ctaatttcag
     1621 agaaggacgt ccgaaagcaa caatcacacc gaagaagagg cacgcatatg aactgcttac
     1681 ttcaggtaaa tcgtataagg aagttgaagc tataaccggt ttcagtcgca gtacgctttt
     1741 tagaataaag aagaaaattg aagaaagtta aaaaagaagt ctcagcgaac cagacaggta
     1801 aatacttaat gcgttctaga tatgagtcta ttagcatgtt gtgaaatata atattttttt
     1861 atttacttga tttctgctca aaaaatgtgt atggtaactg taaaaggtaa agtaattaaa
     1921 ggtatgctag ccacgcttac tatattagga taccatcata tatttataat gaacgatttg
     1981 ttcgtctacc aaacaacaca tatcgttcag cgtaagtcac gtttaggaac gtggcttttt
     2041 tagtgttatt atcgataaat aatctattga ctctattgat tttatctcca tatccctacg
     2101 gtgttgattg tctttagatc tttcatttaa gaaccaaata actatcataa aaatcagcac
     2161 tgctgctaat ataataaaca gcattcctat tgtgatatta cctattgatt ttaccataac
     2221 tacgattaaa gtaataatgg cattcaagtc tcctttgttc tctatatata aattagccat
     2281 aaatcatctt cctttcaaat taagatactt gtttgatacc aataaatagt aagcgtggct
     2341 agcatacctt tttctgtaat agcaagtgtt ctaatagtcc aaacttaatg atggaccgat
     2401 gctagttata gtacagtaat gttttctttc ttatctagat ttttagtaag ctctatcagc
     2461 aaatctaaca aatctgagtc cttttcagaa actaaattaa atctagtgtt tacaacaaag
     2521 ttttcatgtt ctatattgtt tagattattt gcggaaagga gggcttctag atcttgacat
     2581 tcaaatctat atgcatcgtc ataaatctta ctagaaggag tgcttaattc gaaattactt
     2641 attttctcat cttggaccaa gataaattta tagatagtat ctttttgtgt ttttctttta
     2701 aaaacacaac agagtgtgtt ataaggaacg ttataatttt catcagcctc attttttctc
     2761 cattcaaaca aatgataaac tgcaaccaag ttgccatcaa tttcatggac agtgctctcg
     2821 gtaacatcct ttaaagaact atcaagggct aatttatagt aactagagag aggaagtaaa
     2881 attgtcgatt tactgagatg gttatcgtct atacgtgcaa tcatacctaa atcaactaag
     2941 gttttattcc aggtgtttat agttttagtt gtcactccta gagactctgc tatggtattg
     3001 accgaatgcc aggaatcccc gccttcattc tttgccttaa aacagtaata cagatataaa
     3061 ttaatagctt tgttattcgg taaatatttt tcaagttctg ttggtagaat aaagtaagaa
     3121 tccttaatgt tttttctgaa ctgtctccag tctctgtagt tttgtttata aagttgtgaa
     3181 acggtttttc cacctttaga aagtcctgct atcttaacca atcttattgc tcctttcttg
     3241 aatctgtcat gatcatacag tattttttac ttgtagtcca gcaccttaat atattatcct
     3301 atgtaattaa tttcgtacta gatagtaatt attttttatt taattctcta gtcacttaca
     3361 aataagttat ataaactacg attaataaat tattacaaaa actattaaga aaacttctac
     3421 atacaactat tacaaaacta ttaagaaact gaatactcct attttttgtt tttaaggaaa
     3481 gttttttctt ttttgaattt cttatttttt tctaatataa ctaagcaaaa ctggataata
     3541 cgacacttat tttcaaattt tcacaagact aatagtaaat atccatttaa taaagtgata
     3601 caatgtgttg attttacaaa tcaacagaag aagtagggaa ataacttacc tatgcttctt
     3661 tttttgtaca tttaatcgat tcttctttac aaaaacgaac gtatgtttgt ataatgtgga
     3721 agaatggagt gttatttatg atggattatt caaaagagcc agtaaatgat tactttttga
     3781 tcgatatgaa aagcttttac gcaagtgtcg aatgtataga aaggaattta gatccattaa
     3841 cgactgaatt agtcgtcatg agtcgagcgg ataatacagg atcagggttg attttagctt
     3901 cttcaccagc tgcaaagaag aagtacggta tttctaatgt cagtcgacca cgagatctac
     3961 cgcaaccttt tcctaaaacg ttacatgtcg ttccaccacg catgaatctc tatattaagc
     4021 gaaatatgca ggtaaacaat attttcagaa ggtatgtagc agacgaagat ttattgattt
     4081 actcgatcga tgaatcgatc ttaaaagtga ccagatcaat gaatcttttt acgactgaag
     4141 gaacaagaag tcaacggaga aagaagctgg ctcaaatgat ccaggagaat attaaagaag
     4201 agctaggatt gcttgctaca gtaggtgtcg gagataatcc cttattagct aaacttgctt
     4261 tagacaatga agcaaaacat aatgatggat tccttgctga atggacgtat caaaatgttc
     4321 ctgataaagt atggaatatt cctgaaatga cagacctttg gggtattgga tctcgaatga
     4381 aaaaaagact caaccaaatt ggtatcatga gtattaaaga cttagctaat tggaatccat
     4441 acactattaa gagccattta ggtgtgattg gcttacagct gtattttcat gcgaacggga
     4501 tcgatcgaac agatatagcg atcccaccag aaccgacaaa ggaaaaatca tatggtaatt
     4561 cacaagtgtt accacgagat tataccagga gaaatgaaat cgaactggtg gtaaaagaaa
     4621 tggcggaaca agtagctatt cgtatcaggc aacataactg taaaacaggt tgtgtccatt
     4681 tatacattgg cacgtcgatc ttagaaacga gaccaggttt ttcacaccag atgaagatcc
     4741 ctataacaga taatacaaag gaattgcaga attactgtct tttcttgttt gataagtatt
     4801 atgaaggaca agaagtaagg cacgttggaa taacctatag caagcttgtg tacactgatt
     4861 cgcttcagct ggatttattt agtgatccac aaaagcaaat tgacgaagaa aatttagata
     4921 aaattattga caagattcgg caaaaatatg gttttacgtc gattgtacat gcttcttcta
     4981 tgttagaagg cgctcgtagt attactagaa gtacattggt tggtggccat gcaggcggcg
     5041 caggagggct taaaaatgat tgattataat gatcgtggac gtttaaagtg ggcaggtttt
     5101 tatttatctg atcatactga aaaaattgat gccgacaatg aacaacgagc caatcaaaat
     5161 ttggcaaaag aacaaatgac gactgaagag gttgcggaag tattgaacgc tgcagtcata
     5221 aaaaatagat cagtatgtat tcaaaaagaa gaacgaaacg ctgaaggata ctatccagaa
     5281 gatattgtta gcaagatctt aggtagtgat gagctgggga tctacgttgg aaaaaacaaa
     5341 gttcactacg acgaaatacg tcatatcgaa ttttaccagg ataataaatg gggtcgatta
     5401 gaatgaacat tatccagcaa tatgagctaa agtatataac ctttgatcag ctatcagaag
     5461 agatttgggg ttatggccag cgtttgataa atgaggttgg tgttgaacgc ttctctttct
     5521 atgtagaagc tgcagcagga taccataaat acgaatacta tattggctat taaagtgcaa
     5581 taaaattata tttcttttat gttttacatt ttgtatcaat tctgataaaa ttacctatag
     5641 gtaattaaaa aatataaaaa aactagttac aaccttagga cgttgtaact agcttgaagc
     5701 atactagagc taactaaaat agcactagaa agcaatgtta aacccgtcca acaaacttga
     5761 tttaacattg agaccagctt attcagctaa atatagttga ataagcttct tttgttttaa
     5821 aaaaacaaaa ggtgtactac gaataacgca agtaaaagaa atactattac aaccagtagg
     5881 ctacaagtaa gtattactaa acattgcgta gttagaacct ttgctctatt gtctaaagcc
     5941 acaaaattag agatcagctt tatgatgcta agagcgagct gcatccatgc ggacataggt
     6001 tcttttgaca ctattaattc tcctttttag gataagggaa attgctagta gttttttcat
     6061 tagcaccatc ccccttcatt tgattgagtc tagtgctgct gtgatagttt gctgtttaat
     6121 atgcttacat tcttattata acataaatga aacaaacaca tggttagagg tcgtatcaat
     6181 caaaatagtg tctcacaaac gatttattac ttactcctgc tgactaacgg actgttcttc
     6241 agtaactgta actatatgtt tgttaaaacc accattacct gtagataaaa accaatatat
     6301 ttactatact cactatatta aatatattta gtatagtaaa aactagaaac aataaaattt
     6361 gtgaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
     6421 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
     6481 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
     6541 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
     6601 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
     6661 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     6721 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     6781 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     6841 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     6901 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     6961 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     7021 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     7081 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     7141 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     7201 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     7261 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     7321 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     7381 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     7441 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     7501 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     7561 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     7621 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     7681 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     7741 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     7801 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     7861 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     7921 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     7981 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     8041 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     8101 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     8161 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     8221 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     8281 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     8341 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     8401 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     8461 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     8521 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     8581 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     8641 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     8701 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     8761 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     8821 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     8881 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     8941 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     9001 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     9061 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     9121 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     9181 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     9241 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     9301 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     9361 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     9421 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     9481 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     9541 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     9601 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     9661 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     9721 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     9781 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     9841 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     9901 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     9961 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
    10021 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
    10081 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
    10141 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa aaaggaagaa
    10201 gtatatccag tagtagtttt tatttactta aaaaattggt tcattgagca acctatcgct
    10261 gctaaagtag caaaggaaaa cgttaaagga aagaatttag ggaagaaatc gaaatgaaat
    10321 cggacgactg gattgaacac acctggcttc ctctgaaaag aaatagcaaa cgaaagaaat
    10381 tttgggtatt gcaattatgg ccattaggaa aactttgtag ttacggggca cgacttattc
    10441 ttgatttaac cgtttactcg attggagtat ttggaacggc ttattacttg ataaatgatc
    10501 ccaataggat tgcagcatca gacattttta cagtgctaat gacgttgatt tttgtcatgc
    10561 tattgtctat ttttactcaa ttagtcatgc aatttaccag gcaaaaatta acagcaggaa
    10621 aacttttcaa aagcctattc gatttaattg ttgtaaatgc aattctagcg actttaataa
    10681 aacaaattga acaatctgga agaacaacaa aaggagtgaa ggatttttta ttgacgttta
    10741 ttcactttgt aaatggacac atgtatttgt ttgaagtggg tattattctc ttttttgtcg
    10801 ggtatgtatc cacgaaactt attaaacgtt caattgaaaa agaaattgaa attgacctga
    10861 ttccgacact tcttggttat gagtacaatg ttgattttgg aagatatgac gaattgatca
    10921 atacggatat cgttcacacg agtatccatg aatctactgt taagggcatc agatctattt
    10981 taaaaaatgg ccaggaaatg aaagcaatag atcgaagaaa agagatctac aatttttatg
    11041 aagtcgaagc aagcctacga tatggcatcg gaaaattgtg gttagatgga agtaatattc
    11101 gtcaaaacaa accatacatt ttttggaaca gtagtgattt agaacttcca atgaaagata
    11161 aataattatg gagaatcgtt tgaaaatgat tcctctatac aggattgtgc ttatagttgg
    11221 catggagtta aaaaccttaa agaaggagca gttattcaaa gtgaaagaac gtcataatca
    11281 gttactatgt agtaaaacgg gtacgatgaa cattgaatac cgaacagaat atttagaatt
    11341 aacgaatcaa actgattttt tgttgattcg aaagcaacaa gagcaagaaa aaaattaatg
    11401 gctacttata gccaattgat tgatgaatgg gaacaattca aggaatctaa aattccagaa
    11461 cgtctctaaa agcctttatt tgtaaagagc ataaggaaac gtgggaacac tttttaaaaa
    11521 aagtgttcca ataggctgtg cctattacgt ttctcaaaaa taaatgtggg aacaaaatat
    11581 ttaaaaatag gatagtgaaa ggatagtgaa ggatagtaaa aggagggtag taggatagca
    11641 aaaaggatag gctccttttc gctatccttt ttaaattgga ggaattaata tggaagtaag
    11701 aataagaaat gtggatgaga aaatcgtaag agaaattgat cgaagatgtg acgaa
//
LOCUS       DANJMI010000080        11396 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000080 DANJMI010000000
VERSION     DANJMI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.080"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(313..903)
                     /locus_tag="PWU81_002709"
     CDS             complement(313..903)
                     /locus_tag="PWU81_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDL1111344.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1426..1587
                     /locus_tag="PWU81_002710"
     CDS             1426..1587
                     /locus_tag="PWU81_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL1111345.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1606..2100
                     /locus_tag="PWU81_002711"
     CDS             1606..2100
                     /locus_tag="PWU81_002711"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL1111346.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
     gene            2093..2254
                     /locus_tag="PWU81_002712"
     CDS             2093..2254
                     /locus_tag="PWU81_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111347.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
     gene            2738..3761
                     /locus_tag="PWU81_002713"
                     /pseudo
     CDS             2738..3761
                     /locus_tag="PWU81_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            4104..4360
                     /locus_tag="PWU81_002714"
                     /pseudo
     CDS             4104..4360
                     /locus_tag="PWU81_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            4747..4974
                     /locus_tag="PWU81_002715"
     CDS             4747..4974
                     /locus_tag="PWU81_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDL1111348.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            4990..6156
                     /locus_tag="PWU81_002716"
     CDS             4990..6156
                     /locus_tag="PWU81_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL1111349.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6391..7434
                     /gene="hrcA"
                     /locus_tag="PWU81_002717"
     CDS             6391..7434
                     /gene="hrcA"
                     /locus_tag="PWU81_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HDL1111350.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7528..8091
                     /gene="grpE"
                     /locus_tag="PWU81_002718"
     CDS             7528..8091
                     /gene="grpE"
                     /locus_tag="PWU81_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL1111351.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            8142..9971
                     /gene="dnaK"
                     /locus_tag="PWU81_002719"
     CDS             8142..9971
                     /gene="dnaK"
                     /locus_tag="PWU81_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL1111352.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            10122..11288
                     /gene="dnaJ"
                     /locus_tag="PWU81_002720"
     CDS             10122..11288
                     /gene="dnaJ"
                     /locus_tag="PWU81_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL1111353.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         4004 a         1834 c         2333 g         3225 t
ORIGIN      
        1 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
       61 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      121 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac
      181 tgttgccatt ccatctatga atataaatag agtgtatctt ctttgataaa tgtcagaaaa
      241 agtgtgacaa ataaccatca gtaaaattac ttgtcatact attgataagg agtatttaat
      301 tgaagagaat gattaggaga aaggatctcc gtacttttta tagtaatcat agaatccaag
      361 tttgtataga cgaatctttt tttctcttat ttcttctgtt tcaaaatgga ctgcttgaag
      421 aatcaagtct gtgaattcta taggagctgt tccattcgca gtaatcaaat tacgatcttt
      481 cacagcttgt tgttctataa aatcttcttt attctgataa ttttccgatt ctaaccaagg
      541 aaattgcgca tttccggtgt gtttgtaacc ttgcaataat ccgctctgag ctaaataatc
      601 aactgcccca caaatagcag ctactggtac gtctgttttt aacaatttat cgattatttg
      661 atataaatga tgatttttta tgtcccatga attgccaccg atcataataa aaaggtcaac
      721 tttttcaggt atttgttcga ttaagtaatc gatttttgtt tttaatcctc caatggaagt
      781 gacctctgat tggatagaag cagttttgtt ttgccatccc tctttttggt ttagtgtgct
      841 agtaatataa gcaccttccc aatcggcata ttcctctaat agaaacgtaa tcgcagtttt
      901 cattttcaag ctctccttta ctatctatga caaggacaac agttttaaca tacagacatt
      961 cattaaattt ggatagcgac agcgaaaaca acaattctta agttttagac agagtataca
     1021 gacagtcaac ttaattaaat acgaactaaa taaaaatgac aggtaatcat ttttgtttac
     1081 cagcattata acattttgca tacatggatg acaaaagaac caattcccac ttgcttgagt
     1141 acaatcttaa aagaaactgg aaggcaaaaa gtaattatag aaacgaaaaa taaatcatat
     1201 cgggcactca gacatgaaaa caaccctaga aatatataac tagtccaatg aaaaaaaagc
     1261 gaatgccgta gaaaacaagg attctgcgta cattcatatc tcattttgcg agcatatctg
     1321 ctattactgt gactaaatgt taatatggtt aaaatgaccg ttatattttt tatataaata
     1381 tcgtataatt agtatgaata tagaagaagg aaagaaggtg caatcttgga acctaaagac
     1441 aagtatttag attacgctag ggtcaataaa gataaattta ttgaaacaat catcaaggga
     1501 aaaatccctg atgatgaaaa agatgcggta ttcatggctg gaagcccagg agcaggtaaa
     1561 acagaggttg cactaggttt agcttaaaat tatgacaacc atgttgtgat tgatgcggac
     1621 tattttagag cacaatttcc agaatacaat ggaaagaact caagtgtttt tcagaaagct
     1681 tcttcttggt tagttgaaca atcattaaaa tatgtactgg aacatggcta ttctttcata
     1741 ctagaggata cttttgctat ccttagtgca gagaaaaata ttattcgagc ccttaaaaat
     1801 aactttcgcg ttacgatttt ctatgtttac caagatccca aagttgcctg ggatttcact
     1861 cgaaaacgtg aactagctga aggacgtcat gtgccaaaag aaatgtttat caatgcgttt
     1921 tttaagtcca gagagaatat tgaaaaagtc aaaaatagac atcctgaagt agtccttcac
     1981 ataatgatta aggattatca aaacgacatt tctgaagtgc attatgcagc agataatatt
     2041 caattagtac taccaataca atatacttca aaagagttgg aggaggaatt acatgactga
     2101 gaaagaacaa gtacaacaaa tcgtgaagaa gtataataaa agtatcgctg atttatcaga
     2161 aaacgcctca gcgaaagaat ttaaaacagt aatgaattac gtagcagatg aagcaaatcg
     2221 taagcagcga aaactagttg ggttggataa ataaattttt taaaccatat ccttgtaaag
     2281 ttgagttttt tattttaatg aattaagact attaaataga taattattgt aacttaaaaa
     2341 acacagctaa aaattagttg tgttttttat taggtttact tttctggtat aaaaggaact
     2401 agctactttt tcaaaatttc taaatcattc aatatgtagc gcttactaaa ttattcgcca
     2461 taaaatttta taattggatc actcattgtt ttacctaagt catatttaag taataaccgg
     2521 tgtttttaca taagaagact tcgggtattt tttagtttga ttagctctga tatacctata
     2581 aagaatatgc taaggtaaga atagtaaaaa ttcacataga tgaggtataa taatgatatt
     2641 tttatatatt acagataact tgacacaaaa tgagtttttt tcgatttttc aagttatcta
     2701 atgcaattat attgatatca ttaggagctg cattgttatg aatccaacat tgattactaa
     2761 gaaagaatta ctaaaaaaat tagatatttc tactggtgta ttagctaatt taattagaaa
     2821 tggcatgcca aaagagggag aaatgtttaa tcttgataag attattacat ggcgagaaaa
     2881 ttggtctaaa aacatattag gtgagttaga agttggtaga gtatatacta acaaagaaat
     2941 ttcagagaag tttaaatgtt ccaaacaggg tggaatgaga agatctcatc aaactaatac
     3001 tcttgtactg tttagtgatc aaacaggaag taatgtttat aaagataagt ggttaaatgg
     3061 tattttacaa tataccggta tggggctaaa aggggatcaa gtattggata agaatcaaaa
     3121 taaagtttta gccaattcaa aaagtaattt tgtaaagatt catttatttg aaacatttaa
     3181 accaaaagaa cacacatatc tgggagaagt atatttagca ggacaaatat atactgttaa
     3241 tgaaaaagat agttcgggca acagcagaaa agtatataaa tttccattag ctttaataaa
     3301 tcaagagcaa ttgattgaag ataaagatat ataaccagga agaaaatcaa actagacata
     3361 ttcgtaattt atctaatgct aaattagagg aagaagcaag aaaagttagt aattataata
     3421 aaaaaatggc tgcaaaatat gttaacgaag aggagttttg cttctcaaaa acaaaaatat
     3481 ataaacgtaa tcccttcgta tctgaatatg ttaaacgact tgcaaaaggt atttgtcaac
     3541 tttgccaaga aaaagggcca tttataaaag acggtgtacc ttatttacat tgtcatcata
     3601 tagaatattt gtcgcaaggt ggaaaagatg tgattgaaaa ctgtatcgcg ttatgtccca
     3661 attgtcatgc tagaattcat gaacttgagt tacctgacga taaagaaaaa ttgctgaaga
     3721 tcgttgaaaa aagagaaaat agcttttcta aaataccgta atttattttt ataggacaaa
     3781 taagctaatg tgagctatgc cttctatata agactaagtt ctattataag taacgaagta
     3841 attctcgttc cttcctgtag taatattttt taaataacct ataggcttct tcaaaagtac
     3901 atcctccagt aaattctaag tactggagga ttctttttct agtgttgctt tactcatgaa
     3961 aaagtagttg gctacttcat caaaatttct gaactttttt aatgtataat aaagagctcg
     4021 atcatttact acaaggatgc tagctcctac attttcacag ttttttagca agtaaatttt
     4081 tctaaaatca catcaggaaa tgcatgagat tctttaagag aggcaattat agggctatga
     4141 ttacggttcg tgtaagtgac gccaaaaaag aatggctttc ttatatggct gaattttatg
     4201 ggattacttt atctgaactt ctaaaaaact attcgatgga acaactagaa gatgaataca
     4261 atcgtcaaac ggctgaaatt gcccataaac attggataag aagtgggaaa acaacagtat
     4321 tcatggaaga atccttaatg aatttggtgg gctggaatga cctgccgttt aaaattcacg
     4381 cccgaaattc agaagcagct ctgattggca aatacgtgta ttggtagaga tagaagataa
     4441 tcaattcgtt gttatcgcta ttcaagtagg gcaccgaaga aatgtctatg attaagagac
     4501 tgtttatttt tacaaaaaaa taataattgg aatctagtta aaactcttga agtgagagat
     4561 ttgactagat ttttttatga ataaaataag gacaaaattg tttaggaaga atcatgagtg
     4621 taaaaaaatc atccaagaag tcgattcttg gattttttag tggatttgtt cgccaaatac
     4681 gtttagtatg cgtatatgag aggttgtaag caaagcgttg tggtctgagc acttaaggag
     4741 gaatctgtga aaatagcttt tcatattttt gcagattttg acttaatgcc gaaggtcagc
     4801 tttgcgatta ccgtttatcg agaggctgta aaagcaccgt gtagctccga tgacaataag
     4861 caagcatttt tagagctagg tttccttgat tctaaatatg cggcttattt caatgagttg
     4921 gtgcttgaac gcgattttaa gaaggtcgaa aacaggcacg acttttcgac gtaaggagaa
     4981 agaatttcaa tgatagaaac aataaatccc gcagaaaaca aggtttctgc gtacattcat
     5041 atcccatttt gcgagcatat ctgctattac tgtgacttta ataaagtttt tttagaaggg
     5101 cagccagtag atgagtatgt ggaaatgctg ctgaatgaaa tgagattaat gctggaaagt
     5161 catcctacag cgaatttgga aacactttat gtaggaggcg gaacaccaac ttctttatct
     5221 gctaaacaac ttgatcgact attatcaggt gctagagaga tcctgccttt tcaaaatggt
     5281 aatgagttta ctgtagaagc gaatcctggt gatttgaccc gtgaaaaact tcaagtgatg
     5341 aaaaactatg gcgtcaatcg tttatccatg ggagtgcaga catttgacaa tcgcttattg
     5401 aaaaaaattg gacgtaaaca tactgcagaa gatgtgtatc aaacaatgca atttttagaa
     5461 gcagaaaatt ttaccaatgt aagcatcgat ttgatttatg cattaccggg acaaacacta
     5521 gaaggatatc gggatacttt ggaaaaagct ttggctttgg atctgcctca ctattctttg
     5581 tactcattga tcctcgaaaa caaaacaatg ttcatgaact gggtacgaca aggaagactc
     5641 gaactgcctg atcaggaaac ggaaaccaga atgtttgaag agacgattga agcaatggaa
     5701 aagcatggac gtcaccaata tgaaataagt aactttgggc tctccggaca tgaatctcaa
     5761 cataacctca tgtactggaa caatgaccac tattttgggt ttggagctgg tgctagcggt
     5821 tatcttggag atattcgtta ccgcaacaaa ggaccaatcc aacactattt acgcccatta
     5881 cgagcaaatg aacgcccggt attggaagaa gaaaaattga cacgaaaaaa tcaaatagaa
     5941 gaagaaatgt tcttaggact aagaaaaaaa acagggatta ctaaaaaaca tttctatgat
     6001 cgctatcaaa aaacaattga atcggtgtac ggtgatgtgc taagggaatt agagcaagaa
     6061 gggttacttg ttaatgaaga agaccgtgtt tatttgacac caagagggac ttttttgggg
     6121 aacaatgtgt ttgagcgctt tttatttgaa aaatagtttg aagaatattt gttttttatc
     6181 gaaacttgaa aagaaaaagt ttagaaagtt tgacaatgag gtattgaagt attgatataa
     6241 ctggatttac atcaattatt tagaattagc actcttgtgt ttagagtgct aaaaaatgga
     6301 gtctttctct tgacaggaga agggcttctt gctatattta taagtgaatt agcacttaga
     6361 atatatgagt gctaatgaga ggtgaggaag atgttaacaa agcgacaaaa cgacatttta
     6421 cgtctgatca tccaacacta tacgagtagt ggcgtgcccg ttggttccaa aacattaatg
     6481 tctgagggag tcaaggccag ctcagcaacg attcgcaatg atatgaaagc attagaagat
     6541 gaaggattgc tgttgaagac tcactcttct tctgggcgga ttccatctgc tttaggctat
     6601 cgttattacg tggatcattt gttaaaacct gctcgcgtag cagaagatga tttgctgctg
     6661 attcgtcagt ctctaggcag agaatttcat gaaatcaatg agattattaa gcaatctgct
     6721 gagatattat ctgagctgac aagctatacg actttttcat tgggacctga aataaaagac
     6781 cgccgactga ctggctttcg tatcgtaccc ttgaataatc gacagattat tgctatcgtc
     6841 gtaacagata tggggaatgt agaaagccaa gtgttcactc ttcccgaata tatgggaagt
     6901 caggaactag aaaaaatggt acagattgta aacgaccgtc tagtcggaga tcctctgatg
     6961 acggtgtacc ataaacttcg tacggagatt ccgatgattc ttcataaata tttccagacg
     7021 acagaaggga tgtctcagtt atttgacacc gttcttggtc atgtttttga agaaaaggtt
     7081 tatgtcagtg gacaaatgaa tctcttggat tatgagcccc gtcaagatct tgatcagttc
     7141 aaatcgatgt tttcttttat gaaagactca gacggactta cacaaatgat cactccgatg
     7201 gatagcaata ttcatatcaa gattggtgct gaattaggca atgatttatt gcagaatatg
     7261 agtatgattc aagcaagtta cgagatagat ggtcatggaa gaggtacaat agccttgtta
     7321 gggcctacta gtatgcctta ttctcggatg ttcggtctag tcgatgttta tcgaagagaa
     7381 ctagcagcta aacttgctga ttactaccgc tcgcttgact tgtcaaactc gtaatgtgaa
     7441 taacgaagct atttgcagct gatcgttctc tctttcttag taagagcaat caaacagctg
     7501 actgaataat gaaaggaaga ctgtatcgtg aaagaaaatc aagaagaact agataaagaa
     7561 atgacagatg ctcagccaga accggaaatc gatgtcgaag ctgcagaaga ttcgggtatt
     7621 tcagaagctg aagcggaaga attcgaaaca gcgaaattaa aagctgagtt agaagaaatg
     7681 gaagataaat acttgcgggc tcgtgctgaa atcgcaaata tggcaaatcg aggaaaaaat
     7741 gagcgtgagc aattacaaaa ataccgttca caagatttag ccaaaaaatt acttccatca
     7801 attgacaacc tggaacgtgc attagccaca gaagtgtcag atgatcaagg ggcaggtttg
     7861 aaaaaaggcg tggaaatggt cttggaaagt ttgcgtaacg cactggaaga agaaggaatt
     7921 gaaaaaatcc ctgctaaagg agaagccttt gatcctaatc ttcatcaagc agtacaaact
     7981 gttccagcga ctgaagatac accagccgat acgattgtag aagtactaca agaaggctac
     8041 aaattacatg atcgcgtgtt aagaccaact atggtcattg ttgcacaata attgtgcgga
     8101 tataaaactt acactttcaa ttaaagaagg agacgttaat tatgagtaaa attatcggta
     8161 ttgacttagg tacgactaac tctgcagtag cagtattgga aggaaacgaa gcaaaaatca
     8221 tcgctaaccc agaaggaaac cgtacaacac cttctgttgt atcattcaaa aatggagaaa
     8281 tccaagtagg ggaagttgca aaacgtcaag ctgtgacaaa cccaaatacg atttcatcaa
     8341 tcaaacgcca tatcggtgaa gcaggttaca aagtagatgt agaaggtaaa tcatatacac
     8401 cacaagaaat ctcagcaatg atccttcaat atatcaaagg attcgcagaa gactatttag
     8461 gtgaaaaagt cgaaaaagca gttatcactg tgccagctta ctttaatgat gcacaacgtc
     8521 aagcaacaaa agatgctggt aagatcgctg gtctagaagt agaacgtatc gtcaatgaac
     8581 caactgcagc tgctcttgcc tatggtttag ataaaacaga tcgcgacgaa aaaatcttag
     8641 tattcgacct tggtggcggt acttttgacg tatctatcct tgaactagga gacggtgtct
     8701 ttgatgtatt gtcaacagct ggcgacaacc atctaggtgg ggacgacttt gataacaaaa
     8761 tcatcgacta catggtagca gaattcaaaa aagaaaacag cattgacttg tctcaagaca
     8821 aaatggcttt acaacgcttg aaagacgctg ctgaaaaagc gaaaaaagat ttatctggcg
     8881 tatcaagcac acaaatcagc ttgccattta tcacagcagg agaatctgga ccattgcatt
     8941 tggaaatgac attgacacgt gcaaaatttg atgaattaac agctgactta gtagaacgta
     9001 caaaagtacc agtacgtcaa gcgttaaaag atgcaggtct gtcacaatca gaaatcgacg
     9061 aagttatttt agtcggtgga tctactcgta tccctgcagt tgtagaagca gtacgtaaag
     9121 aaacaggaaa agaaccaaac aaatcagtta accctgatga agtagttgct atgggtgctg
     9181 ctatccaagg tggcgtgatc actggtgatg tgaaagatgt tgttttactt gacgttacac
     9241 cattgtcatt aggtatcgaa acaatgggtg gagtgttcac taaattgatt gaccgcaata
     9301 caacgattcc aacaagtaaa tcacaagtat tctcaactgc ggcagataat caaccagcag
     9361 tagacatcca tgtattacaa ggtgaacgcc caatggctgc tgacaacaaa actctaggaa
     9421 gattccaatt gacagatatt ccagcagctc ctcgtggtat tccacaaatc gaagtaacat
     9481 tcgatattga taaaaacggg attgtaaatg tatctgcgaa agatctaggt acacaaaaag
     9541 aacaaaaaat cacgatcaaa tcttcttcag gtctaacaga cgaagaaatc gaacgtatgg
     9601 tgaaagatgc agaagcaaac gctgaagcag ataaagctcg taaagaggaa gttgacctac
     9661 gtaatgatgt agacgcattg ctattctctg tggacaaaac attgaaagaa ttagaaggca
     9721 aagtggacga agaagaagtg aaaaaagcag aaacagctcg tgacgaatta aaagccgctg
     9781 ttgaagcaaa caacatcgag gaaatgaaaa ctaaacgcga tgcattgaac gaaatcgttc
     9841 aaaacttgac tgttaaacta tacgaacaag ctgcacaaca acaagctcaa gaaaatccag
     9901 aagcagctca aggcggagca gatgatgtcg ttgatgctga ttttgaagaa gtcgatggcg
     9961 acgataaata atcagttcac tgaatttcga gagaaggcca aagttgctac tttggctttt
    10021 tcttttgaaa aatagtttga agtatcggtt tttgtttcaa acaaaatatg atatgataac
    10081 aagcgacgtg aaaatgatga gaaccaatag gaggaaagcc tatggcaaca aaacgtgatt
    10141 attatgaagt cttagggctc tcaaaaggag catcagaaga tgaaataaag aaagcttatc
    10201 gtaagctttc gaaaaaatac catcctgaca tcaataaaga acctgatgcg gaagaaaaat
    10261 tcaaagaagt atcagaagca tatgagattt taagtgaccc gcaaaaacgc gctgcatacg
    10321 accaatacgg gcatgcaggc acagatccaa attacggtgc tggtggagct ggtggcggat
    10381 ttggtggatt cggcggcttc tctggtggcg gcttcggtgg ttttgaagat atcttcgact
    10441 ctttctttgg aggaggcggt cggtcagtcg atccaaatgc gcctcgtcaa ggtgctgatt
    10501 tgcaatatac ggtcgacttg tcttttgaag aagcgatttt tggtgtagaa aaagaaatca
    10561 agtataatcg ggaagaaatc tgtcatactt gtggtggaaa cggtgcaaaa ccaggcacac
    10621 aaccaactac ttgtcataaa tgtcatggtg caggtacgat caatgtagaa cgccaaacgc
    10681 cacttggacg agtgatgagc cgtcaaactt gtgatgtttg tcatggcaca gggaaagaaa
    10741 tcaaagaacc ttgtccaacc tgtcatggct caggacatga gaagaaagca cattcagtta
    10801 aagtgaatgt accagctggt gtagaagaag gacaacaaat gcgtttagct ggtcaaggag
    10861 aagctggaga aaacggcgga ccatttggtg atttgtatgt ggtttttcgt gtagaagaaa
    10921 gtgatatctt tgatcgtgaa ggttctgaaa tttactatga attgccttta agttttgtac
    10981 aagcagcttt gggggatgaa gtccaagtac caactgtcca tggagatgtg aaattgaaga
    11041 tccctgctgg aacacagaca ggtacgaact tccgtttacg tggaaaaggc gctccgaagt
    11101 tacgtggtgg tgcaacaggc gaccaacatg tcaaagtaaa actgatcaca ccgaaaaatc
    11161 taaatgaaca acaaagagaa gcattacgtg cattcgctga agctggcggc atcaaagttg
    11221 aagaacagca agaagacggt ttcttcgata agtttaaaga tgcttttggc gggaaaaagc
    11281 ggaagtagtt tttctagtaa aaatataaag tagacttgcc caaattttct atttctagta
    11341 aaaacgctaa gtcattaata attaaaaacc agacgaacaa tcatacgttc gcctgg
//
LOCUS       DANJMI010000081        11331 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000081 DANJMI010000000
VERSION     DANJMI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.081"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(<1..540)
                     /locus_tag="PWU81_002721"
     CDS             complement(<1..540)
                     /locus_tag="PWU81_002721"
                     /inference="COORDINATES: protein motif:HMM:NF026247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HDL1111354.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFK
                     YIFFILVAGLFHNLAYFYLP"
     gene            complement(512..1408)
                     /locus_tag="PWU81_002722"
     CDS             complement(512..1408)
                     /locus_tag="PWU81_002722"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL1111355.1"
                     /translation="MKNKIGIIIVTYNPDNNIKLLVDTLIQEKQNKLIIVDNGSDNIN
                     SFQFEMMENLILKKMNENIGLATAQNIGIEIAKKEKCDYILLLDQDTIIQKNLSLKLL
                     EEFTNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMKIAFAN
                     SSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHVGDGEN
                     LKIGSKKIKIDNHNSTRKYFIARNSTLVKRMYKSDKEFSRLMNKRLLKFFIAIAFETD
                     KLSRFRAAIRGVRDGYRGVIGD"
     gene            complement(1398..2558)
                     /locus_tag="PWU81_002723"
     CDS             complement(1398..2558)
                     /locus_tag="PWU81_002723"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111356.1"
                     /translation="MSENKKKWLLIHPFDYISRTNILEEILTENSIEYFNVVSDYSHI
                     RKQFLSSKERHMIKNRHVIKTIGYKKNISVMRILSHIWFSIKTLLFIRKEKPEIIYCR
                     IPPNTLVFFIAKFSRCNRVIFDVYDLWPESMPGIGNKYKFFTDLWKKLRDQYLSNSSL
                     VITECDYFKKFIKISGESKNMTIYPCSFNVDDQNDYGEKIVNKSLESTLNLAYLGTIN
                     NIVDLEAINELLKTCKRKFGNKINLYFIGKGESKTKLADICEKNHIEFVDCGVIYDNA
                     EKKVILDKCHFGLNLFKSNTIIGLSLKSIEYFRFGLPIISNLSGDTKEIVKRYNVGIN
                     YNSTVDENEMLKFYQCNKNINENVRKIYATIFSEQVLKMKWNEVYKVMNDEK"
     gene            complement(2551..3165)
                     /locus_tag="PWU81_002724"
     CDS             complement(2551..3165)
                     /locus_tag="PWU81_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_065828827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL1111357.1"
                     /translation="MYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGSPVIFK
                     QQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPELWNIL
                     KGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFEKDIEY
                     VENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            complement(3173..4255)
                     /locus_tag="PWU81_002725"
     CDS             complement(3173..4255)
                     /locus_tag="PWU81_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_037946024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL1111358.1"
                     /translation="MSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEEELAEYVGASHAA
                     ALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPVFVDSDYETWNMN
                     PEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIEDAAESLGTTYKGK
                     YTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSREKARHYQHNEIG
                     YNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQFMPENNWNEPNYW
                     LSVITVKGINTNHLIDELDKNNIEARPIWKPMHLQPVFDKYEYIGTDVAQTLFEKGIC
                     LPSDSKMTDDDLYRVTNTIKRVMQNV"
     gene            complement(4293..4925)
                     /locus_tag="PWU81_002726"
     CDS             complement(4293..4925)
                     /locus_tag="PWU81_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL1111359.1"
                     /translation="MKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSLKMQNQELVG
                     KTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVASSALVGINV
                     QLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVNISGHVLLGD
                     KNEIGVGTKIIQNLRIGNMNIIGAGSVVIKNINNKTKNVGVPTKVIERWD"
     gene            complement(4936..6753)
                     /locus_tag="PWU81_002727"
     CDS             complement(4936..6753)
                     /locus_tag="PWU81_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL1111360.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            complement(6766..7530)
                     /locus_tag="PWU81_002728"
     CDS             complement(6766..7530)
                     /locus_tag="PWU81_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HDL1111361.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(7558..8256)
                     /locus_tag="PWU81_002729"
     CDS             complement(7558..8256)
                     /locus_tag="PWU81_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HDL1111362.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            complement(8268..9047)
                     /locus_tag="PWU81_002730"
     CDS             complement(8268..9047)
                     /locus_tag="PWU81_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDL1111363.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            complement(9063..10007)
                     /locus_tag="PWU81_002731"
     CDS             complement(9063..10007)
                     /locus_tag="PWU81_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL1111364.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(10053..10832)
                     /locus_tag="PWU81_002732"
     CDS             complement(10053..10832)
                     /locus_tag="PWU81_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111365.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
     gene            11175..>11331
                     /locus_tag="PWU81_002733"
     CDS             11175..>11331
                     /locus_tag="PWU81_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL1111366.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGF"
BASE COUNT         3257 a         2245 c         1799 g         4030 t
ORIGIN      
        1 agggagataa aaatatgcaa gattatggaa cagtccagcc actaagataa aaaaaatata
       61 cttaaatata gctttgctgc ctcgcaattt agttagatga taaaaagcta gtaatatgaa
      121 tatcaatgcg ctaaacatac gtacttgtat tgcttgtgaa gtaaaaccaa aaataaaaaa
      181 caagaacaaa actaaaaaaa cattagttgt gaataatctg atgacataca ataaagctga
      241 gagtaagaca gtagaaataa tcaataaaaa aatctgatac tgactggaaa taaaggtaca
      301 tacgtacata acagctctga atcccggttc aatactatct gaactaaagt ccataccaaa
      361 ttgtgctatc ccattataca agacagaata tgcataataa tcagcgtttt gtgtgttaaa
      421 tgccatgatg atccacatta tcaaaagtag caccccagac aaaatcttgt tcgatgtact
      481 ccattttttt tgtgaaacac tcttttcttg attaatctcc aatgacgcct ctgtatccat
      541 ctcgaacccc ccttatagcc gctctaaatc tactgagctt atcagtctca aaagctattg
      601 caataaaaaa tttcaataat cttttattca taaggcggga aaattcctta tcacttttat
      661 acattctttt caccaatgtt gaatttcttg caataaaata ctttcgtgtt gaattatgat
      721 tgtctatttt aatttttttt gatcctattt ttaaattttc tccatctcca acgtgatgat
      781 aaaaatagac ttgatttgtt acataaatgt tatagccatt atttgcaacc cttttacaaa
      841 attcaacatc aatctgatca ataaaaaatt catccttcat atttccaaca tcgtcaatta
      901 attcactttt gaaaaacatt ccagaggaat ttgcaaatgc tattttcatg gaaccactag
      961 ttgaagcaaa gtgttttttt acgtatctcc ctctctcatt tttgcaggta aaagagacag
     1021 attcatcatt gctattctca aagtaattgg gaacaataat cccaatgtcc ttatctttcg
     1081 cttcaatatt ggtaaactct tctaataatt ttaaagaaag attcttttgt atgatggtat
     1141 cttgatctag taaaaggata taatcacatt tctctttttt ggcaatttca atacctatgt
     1201 tttgagctgt cgcaagccca atgttctcat tcattttttt taaaattaag ttttccatca
     1261 tttcaaattg aaaagagtta atattatctg agccgttatc cacgataata agtttgttct
     1321 gtttctcttg tattaacgta tccactaata actttatgtt attatctgga ttgtaggtaa
     1381 caataattat cccaatttta tttttcatcg ttcatcacct tgtacacttc attccatttc
     1441 attttcaata cttgctcaga gaatatagtg gcgtaaattt ttctaacatt ttcatttatg
     1501 tttttattac actgataaaa tttcaacatc tcattttcat caacggtact attataattg
     1561 attccgacat tatatctttt aactatttct ttcgtatctc ctgataaatt tgaaattata
     1621 ggtaatccaa acctaaaata ctcaatcgat ttcagactta aaccaattat ggtatttgac
     1681 ttaaataaat ttagtccaaa atgacattta tcgagtataa cttttttttc tgcattgtca
     1741 tatataacac cgcagtcaac aaattcaata tgattttttt cacaaatatc agctaattta
     1801 gttttgcttt caccttttcc aataaagtaa agatttattt tgtttccaaa ttttcttttg
     1861 catgttttca ataattcatt aatcgcttct aaatctacga tattattaat cgtacctaaa
     1921 taagcaaggt tcaacgtaga ctctaagctt ttattgacta ttttctctcc ataatcattt
     1981 tggtcatcta cattgaaaga acatggataa atggtcatat tttttgattc tccggaaatt
     2041 tttatgaatt ttttgaaata atcgcattct gttataacta gactagaatt actgagatat
     2101 tgatctcgta gctttttcca taaatcagta aaaaatttat atttgttccc tattcccggc
     2161 attgactctg gccataaatc atacacatca aaaataactc gattgcatct actaaactta
     2221 gcaataaaaa aaactaaggt attaggtggt attctacaat aaataatttc aggcttttct
     2281 tttcgaataa ataataatgt ttttattgaa aaccatatat gagataaaat tcgcattacg
     2341 gatatatttt ttttatatcc gatcgttttt atcacatgac gatttttaat catatgcctt
     2401 tctttactgg atagaaattg ttttcttata tgtgagtaat cagatacaac attaaaatat
     2461 tcgatactgt tttccgtcag aatttcctct aatatattcg ttcgactgat ataatcaaaa
     2521 ggatgaataa gcagccactt ttttttattc tcactcatct atcttcgcac cctcaaattt
     2581 ctcagtagtc ccacttgcta ctgatgttat tccatcacgg acaataactt tctttagcgt
     2641 taaaaaaaat atttttatgt ctttcatcat tgaaatgttc tccacatatt caatatcttt
     2701 ttcaaatttt tctttccaac taattgcatt acgaccactg acttgagcta atccggttaa
     2761 acctgggcgc acaaaatgtc tttttcgttg ttcattactg tatagtgaaa gatactcaac
     2821 taacagagga cgcggtccaa taatactcat atctcctttt aaaatattcc ataattctgg
     2881 tagttcatca agactagtcg aacgcaatat tttaccaaat tgagtcaatc tgatatcgtc
     2941 tgataataaa ttaccttctt catctctttg atctgtcatt gttcgaaatt tatacatcag
     3001 aaaaatcttt tcatgttttc caggtcgttg ttgtttaaag atcactggcg atccaagctt
     3061 tattcgaact aggattgcaa caaacgtcat cagtggccat aacattatta aacctaaaat
     3121 tgaaaaaaga atatctatca aacgtttaaa gaacttttcg tagatacctc tattagacat
     3181 tttgcatcac tcgcttaatt gtattagtta ctcgatacaa atcatcatcg gtcattttag
     3241 aatctgaagg taaacagata cctttttcaa acaatgtctg agcaacatct gtaccaatat
     3301 actcatactt atcgaaaact ggctgtaaat gcattggttt ccatatcggg cgggcttcta
     3361 tattattttt atctaactca tcaataagat gattcgtatt tattcccttt actgttatga
     3421 cacttaacca ataatttggt tcattccaat tattttctgg catgaattgt atatttgaca
     3481 atttagacaa ttctttttta taaaaactat gaatatatcg ttttttctca attctttcat
     3541 ttaaaacttt caattgtcct cgaccgatac ctgcaacgat attgctcatt cgataattat
     3601 agccaatttc attgtgttga taatggcgtg ccttctcacg agattgcgta gcccaaaacc
     3661 tcactttttc caccttttct ttgtcattag acacaagcat tccgccacct gaagtcgtga
     3721 ttatcttatt tccattaaaa gaataaattc catagtcgcc gaatgtacca gtatattttc
     3781 ctttatatgt tgttcctaat gattctgcag catcttcaat taaaacaaca ttatgttctt
     3841 tgcatatttt agtaatttca tctagttcag ctgataaacc gtacaaatgc actactaata
     3901 cagcttttgc gttaggatat ttttctagcg ctgttcgcaa atgttcaggg ttcatattcc
     3961 aagtttcgta atcactatca acaaatactg gaatagctcc ttgatagatg attggattgg
     4021 cagtagctga gaacgtcaaa gactgacaaa tcactatatc atctttaccg accccagccg
     4081 cttttaaagc tagatgtata gctgctgttc cagaagatag tgcagctgca tgactagcac
     4141 cgacatattc tgctaattcc tcttcaaaac cattgacatt catacccaaa ggagccaccc
     4201 aatttgtatc aaatgcttct tttacatact cttgttcata tccttcatca ctcatatgag
     4261 gagatgctaa taatattcgt ttattttcca acttaatccc atctttctat gactttggta
     4321 ggtacgccaa catttttagt cttattatta atatttttta ttactacact tcccgcacct
     4381 ataatgttca tatttccaat tcttagattt tgtattattt tagtacctac tccaatttca
     4441 tttttatctc ctaatagaac atgtcctgaa atatttacac ttggaaaaat tgaattataa
     4501 ttaccgattt gagtatcatg tccgatagta gagccgatat tgatcatatt gaaatttcct
     4561 aaaactacgt cagcagtgta agtcgtatag ggcatcaaaa tattgccaat tcctaactga
     4621 acatttatgc ccactagtgc acttgatgca actaaattag ggaaaatcaa tttattattt
     4681 ttatttatct tttgatatat tttctctttg atatctggcg aagcgattcc cagaaaaata
     4741 gcagtttctt cttctaattc taataacgag tctattgttc ccaatacagt ttttcccact
     4801 aattcttgat tttgcatttt taacgaatca tcgacaaaac caatcaattc atatctagag
     4861 aaactttcta gtagaaaagc aatctctttg ccaaatccac cagctcctac aataattgct
     4921 ttcactttac tccccctact tattcgaagc attcgcaaaa gtgacaatgt ctttagccaa
     4981 ttgttcatcg tcttgcggca ggctcttgac aaattccatc actgtttgca tggaataacc
     5041 tttgatgttt cccacaaaga tcttctcgta aactgcttca tcgtttcttt ccttatctaa
     5101 caatagttct tcatatagtt tttcccctgg acggatacct gtttcaatga tttcgatctc
     5161 gtcttcgcta tatccgctca atcggatcat gttttttgct agatctacga ttttcactgg
     5221 ttcactcata tctaaaataa agatttcgcc gccttttgct aacgccccag attgaataac
     5281 taaacgactt gcttcaggaa tcgtcatgaa atatcgggtc atacgaaagt ctgttacagt
     5341 gactggccct ccttgagcaa tttgttcccg gaatactgga atcacactac cgcgagaacc
     5401 aaggacattc ccaaatctga cagctgagaa tttcgtacat ccttcttcat tcaacccagt
     5461 cacgatcatt tcagcgattc gttttgttga acccatgacg tttggcggat tgttggcttt
     5521 atctgtcgag accatgacga agttttttac tttcgctgct ttggctgctt ctgctacatt
     5581 ttttgtaccg aaaatattgt ttttgactgc ttcttttggg ttatattcca tcaatggtac
     5641 atgcttatga gcggctgcat ggtagacgat atctgggtgg tattgttcca tgatctcatt
     5701 gattttcttg cgatcttgga tatccgcaat gatcggcacg atttctgtag gacacttttg
     5761 atgatgcgtg cgtaattcac ggtcaatcaa gtaaatcgag ttctccccat gacctaaaag
     5821 taatagtttt gcagggtcaa attggatgat ttgccggcag atttctgaac cgattgatcc
     5881 tcctgcaccg gtgacaagaa tgactttgtt tgtgatctgg tctttgatgg attcgatatc
     5941 caactctacc tcatctcttc ccagtaattc cactacgtcg atggttttta attttgatac
     6001 gctgattttc cctgaggcaa gttcttccat agaaggcatc gtattgactt tcacatgagc
     6061 agattcgact aactcgaaga ttcttcgtaa tttttttctt gataaagaag ggatagcgat
     6121 cgttaccatt tggatgtcat atttttcgat caattccggt atatctttta atgaccccag
     6181 tacttttttt cctgaaagat aggtatttct tttattcgga tcatcatcca cgaatccaat
     6241 gacatggata tcttgagccg ttttagaacc aagaaagctg ttgtataaaa tacgtccacc
     6301 ttcgccggcc ccaacgatca aggtattttt agcggaatct gttgatagat acctcatatt
     6361 ttttgtttcg atatagatac gccagatcaa tcggctgcca atgatcagta atagcgaaag
     6421 taggtatgca aagatcacta aacgtaaact gtattgttta tttatgaaaa gcaatatgat
     6481 gatacttgag gccgcacttg cagataacga accaaaaatc gcaatgatct ctcttaaatt
     6541 tgtataacga ttgattcgag taaaaacttt gaataaacta ccatagaata aataaaaacc
     6601 aatcgacaaa ccgatagaga ttaaaataag gtccattggt atagctacaa acggcttcat
     6661 gaatttagct gctgcaatat tcgcagcaat caacaacaag ctgtccacaa ataccaatgt
     6721 ggcaattttt cttcttcttg ttaatacaaa caccctacat tccccctaaa ataaaccaaa
     6781 cttcttcttt tttacctctt catagactgg ataactgatc tcatccccgt tgatcaggtc
     6841 ctttgcgact tgtttcatct gtgctacttt ttccttacca aagtccttgg cgatttgctc
     6901 ataacattct tttaagtaaa aggtacgttt agagacccca tgggcatcgg aagcagccat
     6961 ttgcacgagg ttatgtttga ccatcatttt ggctgttttc tggatgtctt ttccaaatac
     7021 accgacataa cttggtgcgg tgagttgagc caagcatccc atatctagga atggcagcaa
     7081 atgatttggg tcttctcgaa aatgcgcgtt gcgttctgga tggacgatca ctggtgtctt
     7141 tcctagttgt aacaactcaa aaaagagcca ttctgcatat aacggcactt ccagtgtcgg
     7201 aaattcgatt agaagatatg tatcatctaa atctgtaaat aaaatttcat ctcggtggat
     7261 gtcttcaatc agtgttccgg taatacgtac ttcttgtcct tccaataacg tcaaaggcaa
     7321 ttgtcttttc tctaattctg cttgtaacga agcgactaga gaaatgactt gtgatttttc
     7381 attgctgtat tttccattat tgtgatgggg tgtgcataaa atatgtgtga tcccttgttg
     7441 aatcgctttt tcagccatgg cgatactggc atctatgttt tctgctccgt catcgattcc
     7501 tggcaggata tggcaatgta gatcgatcat ttctcttgtc tcctctcttt ttatttctta
     7561 attcccgtag taatagccag catctctgct gtgttctgct ccattgtata caacaccaag
     7621 gactcgcgct tgtaccatat ctaacagttc ttttgcttta gtcaatgatt cttttcgtga
     7681 cgtattttcc cgtacgacca ataaggtgcc atccgttttt gaagacatga tctgtgcatc
     7741 tgttacggca acgactggtg gcatgtcgaa aatcactaca tcaaatagtt gtcttgcttc
     7801 tgctaaaatc tgatccattc ttggcgaact tagtagttct gatggatttg gtggtttagg
     7861 tccacttggc agaatcgaca aattgtcgac tggtgttcgt tggatctcat ctgctacact
     7921 tcctgatgaa ctaagaacag tacttaatcc gcttgcgttg ttcaacttga acgttttata
     7981 gatgacgggc ttacgtaagt ctgcatcgac tagcagtacc cgttgaccag atttggcaaa
     8041 aacaacggca atgtttgctg cagtggtgga ttttccttct ccaggacctg atgaagtcac
     8101 gacaatcgtt ttgatttgct gaccagctgc ggaggcaaac tggatattcg tccggatcgt
     8161 acgatattgt tcggcgattg gtgaagatgg gtttgttaat gtgatcaaac tgactgcatg
     8221 agtttgagtt gctttttgtt tctgtgttcg tgccattttt cttcctccta gactcttgag
     8281 cggcttctgc gtgatggggc tgttttatcg tttgtagctt ttggtcgtgc aggagctgat
     8341 ccttttttac ggataccagc gttcaattct tttgctgaca tttctggaac tgttcctaag
     8401 attgggaatc ctaatgtatc cgtcacatat ttttcatctt tcactgtccg atcaagtaat
     8461 tcgaataaga atgccagacc tacgcctacc atcattccta acactaagcc aatcactaga
     8521 ttcaatttat tatttggcga aacaggtgtc gtattggcaa ctgcacctga aataatcgag
     8581 attttatcga cattcatcac gtctttggcg ttttcttgga aaacttgtga agttgtatta
     8641 gcgatctgtt gagcagtcac tgcattcgtg cttattgcct ggatcgagaa catttgtgaa
     8701 ttttgtgact gattgacgga gatcgcatct ttgatttgtc cagcagtcat tttcacattg
     8761 tcttctgttt ctaaacgatc tttgacttca tttagtacta aatcactgac gatcatatct
     8821 ttatacgtat tgatcatttg caggttcgta ttgacatcat tgacatttgc tgtgtctgat
     8881 tgtggcagtg tcacgatcaa ttgtgcttgt gaactgtatt ttggtgtcaa gatgaaaaat
     8941 gttacgattc ctgagatggc caagccagca aacattgaga tgatgattgt taccaagtgt
     9001 ttgcgtaatg tttcaaaaat ttctttcaag ctgattgtct cttccatttt ttctccactt
     9061 tcctatttat tctctagatt catttgatct tttaattctt ttttcacacg atcaagttct
     9121 tgatcgctga ctttttgata ggagatatta tcttgcatga agccttctcc ttgcagctga
     9181 tcttgcttga tcgtaccaaa tgcctcccga taatctaaag cgattttttt catttcatca
     9241 aagctcatgt ctgttttcat attcgactct agagcattca aaacggattg atagttgctg
     9301 acaccgttta aactcaatac tttttttgct acaccttcta cgatctttct ttgacgctct
     9361 tgtctaccat agtcaccatt tggatcttca taacgcatac gagaataaga taatgcctga
     9421 tccccgttca atgtaatttt accgattgga aagtcatagc catcttgtga gaaagtgagg
     9481 tcattattga cttcgatacc accaactgca tcaacgagtt cctttagccc tttcatattg
     9541 atagagacat agtgatcgat tgggatgtct acatattttt gtagggtatc catcgccatg
     9601 gcagctccgc cgaatgcgta agcgtggttg atcttgtctt ttgtgtcacg acctacgatg
     9661 tcgacatacg tatctcgagg aatgctgacg atcgttgtct gtttgtcttc aggactgact
     9721 gtggcaacca tcatcgtatc tgatcttcct tgatcgaccc gacctaaatc tccagtatca
     9781 atgcccatca atagaacaga gaaagattct tgtttgtcca gatcaactgg tttcgttcgt
     9841 tctccttggg ttcgttccac tgattcgtaa gttttctcaa tagaatgtga gagatccaga
     9901 tataattttg ctcctactcc tacaattgct aatactaata cgataagaac tgctaaaata
     9961 atcagaaata tttttttccc tcttgatttt cttcttcgtc ttgccatagt ttcctccgtt
    10021 tgctaacctt gcttgaatca acgttttaac tgtcaataac ttatattgct atagcccatt
    10081 tgctgagcaa cagtttgtgc ttttgttgcg tatgctgcgc ttattttttc attccggatc
    10141 tgctctattt gactgagcgc cgtcaaatat tgatcgtaag tagcggtggc agtcacttga
    10201 ccatccttca acatcgtatc gaataactga tctagttgcg tgacttgcgc gatctgagta
    10261 tcagcgaatc ctagatattg cataacaacc gttttccaag gactatcttt aaaaaagctc
    10321 atattttcac gtacacgagc gacatctgca cttgtcacct tttctttgat gacgacatcg
    10381 tctacagctt gctgccagtt tgatacattt ttagtaaata aatcgttgac agctgtctgg
    10441 atcttcagtt ttgattctgc actatctaac tgtgtcacga ttgctttctt ctcatcttgg
    10501 attgcaacag cttctttagg caattctgat tctttgatgt cgtaatcatc tgctgaaagt
    10561 ttgacagagg atagcttatt tgacaaactt gtgattgttt cttctgttat ttcagggttt
    10621 agaaaatttt tttcttttgt actatacagc ttgtctactt cttttgataa agaagtcaag
    10681 gtgtcttttt gattggtgat ggcagattct gcataattga tccgttgctg atgttgccgc
    10741 tgattattga tatggtaacc aaacaaacag atcgcaatag cacctgcacc taccgccaca
    10801 gcagtcaatg acttgacgaa tggcttttcc atagattcac tcctaagtat tgatgataga
    10861 taaattaaga tataattcac atacataaaa atgacagctt tttattttaa aaaacataat
    10921 taaattaata tattcacaga aatatttgta aacaagtttt ccctatataa tcataccaca
    10981 aattgttttc ttgactaggg caatttacga tttaaaagta aataaaacat taataagata
    11041 acaattaatc gatttcattt ttatttcctt aattttcata gagagtgtaa aatattttgt
    11101 gtaaatagaa aaaaggaagt cccttctgta gaatagagtt accacaacac attcacagaa
    11161 aagaggactt ccatatgaac gattttacta cagaaattct aaagactcta gcgaacaaag
    11221 gcgatttgaa tgaattattc cgtgtccatt tggaaaaagc tgtcaatacg cttctcaaaa
    11281 cggagttaac ggctttcctc gattacgaaa agtacgatcg cattggtttt a
//
LOCUS       DANJMI010000082        10218 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000082 DANJMI010000000
VERSION     DANJMI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.084"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..158
                     /locus_tag="PWU81_002734"
     CDS             <1..158
                     /locus_tag="PWU81_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111367.1"
                     /translation="LNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRK
                     LVEYGII"
     gene            145..1566
                     /locus_tag="PWU81_002735"
     CDS             145..1566
                     /locus_tag="PWU81_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL1111368.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            1579..2586
                     /locus_tag="PWU81_002736"
     CDS             1579..2586
                     /locus_tag="PWU81_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL1111369.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            2598..3602
                     /gene="neuB"
                     /locus_tag="PWU81_002737"
     CDS             2598..3602
                     /gene="neuB"
                     /locus_tag="PWU81_002737"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDL1111370.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            3599..4747
                     /gene="neuC"
                     /locus_tag="PWU81_002738"
     CDS             3599..4747
                     /gene="neuC"
                     /locus_tag="PWU81_002738"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDL1111371.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            4720..5397
                     /locus_tag="PWU81_002739"
     CDS             4720..5397
                     /locus_tag="PWU81_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL1111372.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            5387..6529
                     /locus_tag="PWU81_002740"
     CDS             5387..6529
                     /locus_tag="PWU81_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDL1111373.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            6542..7519
                     /locus_tag="PWU81_002741"
     CDS             6542..7519
                     /locus_tag="PWU81_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL1111374.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            7519..8427
                     /locus_tag="PWU81_002742"
     CDS             7519..8427
                     /locus_tag="PWU81_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HDL1111375.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            8428..9180
                     /locus_tag="PWU81_002743"
     CDS             8428..9180
                     /locus_tag="PWU81_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDL1111376.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            9185..10132
                     /locus_tag="PWU81_002744"
     CDS             9185..10132
                     /locus_tag="PWU81_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDL1111377.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         3693 a         1289 c         1902 g         3334 t
ORIGIN      
        1 ttttaaatga atatacagca ttatctgaag ataaaaaaga aacattgaca aatattgttc
       61 ttttctgtga acattataac cgtctatcag aagaaaaaat tttactccca gaaaactggg
      121 cagaattaag aaagttggta gagtatggga ttatttaaca cactttccaa gtcaaaatta
      181 gttaagtcca gtttctggta tactataggg aattttttta ttaagggaat ttcattcata
      241 actattccta tttttgtaaa tattactact caagcagaat atggtttgat taataatttc
      301 acttcatttg tatcaatttt ttcgattctt gttgggctaa gcctaaattc ggcaattaat
      361 aatgcaagtt ttgactataa agatgacata aatgaattta tgtcatcggt actttctcta
      421 gctacacttt cgttaattgt gttcttatta atagggaaca tatatttttt gttttcaaat
      481 gctttttttg atatgaacca acttgtattt aatttgatgt tattacaaag ttttgctagt
      541 tttgtaatta gttatatcca gtcttatttt acgattaact ttaaccattt taaatatttg
      601 attttatctt ttatatctac ttttttgaat attgcaatct ctgtagcttt gatgcagaca
      661 ttatattttg atgataaata tgttggcaga gtagtcggcg gaacactgtc atttgtgttg
      721 ataggtttag taatttattt aagaataatg ataaagggtg ctcatttttt tgataaaaaa
      781 cagtggagat acgctttaaa aatctctttg ccgctaatcc cacattcctt agcaaatata
      841 gttttagcac aattcgatag aattatgatt aattcttatt taggtcctgc tgcgggggga
      901 atatattcct acattagtaa cataggtatt attctaagcg taatatggat gtctactaat
      961 aacgcctggg ttccatggtt ctatggtgag atgagtaaaa ataatgaaaa aaaagtgaag
     1021 aaagttagta actattattt agtagtcttt actttgatta cgatgattgt aatggtagtt
     1081 tcgattgatt tagcaaaatt tatggctccg aaagactatt attctgggtt atctttagtg
     1141 gttccgataa ccttaggcta tttctttcag tttttatata gtttaccagt taatgctgaa
     1201 ttttatgaga aaaaaacatc gtttattgcg attggtacag tagcaagtgc tatagttaat
     1261 atactattaa acatgatttt catacctagg ttaggaataa ttgctgctgg atacacaacc
     1321 gtatttacgt attttttact ttttattttt cattataata tcgctaaaaa aattacgaat
     1381 aaacaattat ttgacacaaa aaaaatatgt atcataataa tttttgtatc aattttttct
     1441 ttgatacttc atattttaat caatcaaatg ttgattcgtt acgttttgac atcattattg
     1501 ataattagtt tagttatata ttttttaaag attaagaatc gaattataga tgactataat
     1561 tcataggagg agatataagt gtttaaagga aaaacaatat tagtaactgg cggaactggt
     1621 tccattggaa gtgaaattgt tcgtcagctt ttaaaatatg aaccaaaagc tatcagaatt
     1681 tatagtagag gtgaagaaaa acaattttat atgcaacaag agctctcaaa tcacactaat
     1741 ttgagatttt taattggtga tatcagagac tctcaaagga ttgattatgc atgtcgtgat
     1801 gtggatatta tttttcatgc tgccgcaatg aaacacgttc cagcaagtga atataatcct
     1861 atggaagctg taaaaacaaa tgtgatggga actcaaaatg taatagatgc agcgatcaaa
     1921 aataatgtaa aacattttgt aggtatcagt acagataaag tagtgagtcc tactaacacc
     1981 atgggtgcga caaagttatt gagtgaaaaa ttaataatag ctgccaatca gtataaagga
     2041 aaaacagaaa caatattttc ttgtgtaagg tttggaaatg tcatggggtc aagtggatct
     2101 gttattcctc tttttagtaa acaaataaaa aacaataaag atattacttt gacagatgtt
     2161 gatatgacac gatttatgat gtcaataccg gatgctgcaa gattagttct gtctgctgct
     2221 atgaattcaa aatttggaga aacatatgtc ttaaaaatgc cggcaatcaa tattttaact
     2281 ttagctcaat cattaataaa aatatataat gatacacatg atgaaaaata tagaggtgaa
     2341 ataaaagttg taggtatcag gccaggagaa aaattatatg aagagctaat gactacagac
     2401 gaaattgaac gctcatatga aaatgataaa atgtatatca ttccaccaca ctatcatgct
     2461 actgacgaac tgtacaaaga ttatgaaaaa attaaaatca ctgcatactc ttcagatgca
     2521 acagaattaa tgagtggaca ggaatttatt agaatcgcaa aagagaataa tgtcgtgact
     2581 tggtaaggag ttttatgatg aaatcagaag tatatgtaat tgcagaaatt ggtgtaaacc
     2641 ataatggaag tctagattta gctttagaaa gtattgataa agctaaagaa tgtggagcta
     2701 acgcggttaa atttcaaaca tttaaaacga ataagctagt ttccaaaaag gctccaatgg
     2761 caaaatatca gaaggataat cttaaagagg acagttctca atttgaaatg ctaaaaaaat
     2821 tagaattatc tcaatctgat tttattagaa taaaagatta ttgtatacaa aataaaattg
     2881 attttttgtc tactcctttt gatgaagaaa gtgcagactt tctaaaacaa attggtgtac
     2941 aaggatttaa aattggctca ggagatttaa caaatttacc ttttcttaga aaaatagata
     3001 tgtatgggtt gccagtttta ttatctacag gtatggctga tattcatgag gtcagtgagg
     3061 ctgtagattg tttcatagat agtccagtta ctattttaca ttgtacttca aattatccgg
     3121 ctagttctga agatattaat ttattggcga ttaacacatt aaaagaaatg ttcgggatac
     3181 ccgttgggta ctccgatcat tcattgggat atgatgttgg tatttgtgct gttgcgatgg
     3241 gtgctaaggt aattgagaaa cattttacct tggataagga actacctggt ccagatcata
     3301 aagcttcttt agatcctcag gaatttgcag attttgtaaa gcatattaga aacactgaaa
     3361 tatttttagg tgatggtgtt aagcgagcaa tgccttctga acttagtaca aaagaagtat
     3421 caagaaaaag cctagtggtc actgagaatt taaatgtggg agatattctt acagagaaat
     3481 ctcttacaat aaaacgtcct ggaaatggaa tagctcctaa gtttttatat caatatattg
     3541 gcaaaaaagt taaaagacca ataagtgcag atagtgttct gcttgaggaa gatgttgaat
     3601 gaaaaaaata cttatagtaa gtggaacacg agctgattat ggaatatata aacctgttgc
     3661 tgagaattta ctacaagata attgggatgt atcatttgtt gtttctggaa tgcatttatc
     3721 ccataaatat ggatttacta aagaattgat cttagctgac ggatttagaa ttagtggaga
     3781 agttcaaagt ttgtttttag agaacaataa gggaaacatg gctcggtctg tggcattaga
     3841 gataaatggt ttcacaaata tatttgaaca aactagccct gattttgtat tattactagg
     3901 ggatagagga gaaatgttgg cagctgcgat agcttgtttg tatcttggaa tcaaaacagc
     3961 acatattcac ggaggcgaag tgtctggtac tgtagatgaa tcaataagac atgccataac
     4021 gaaaatagca tctgttcatt ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat
     4081 gggggaagat tcttggagag tttttcccgt cggagctcct agaatagata ctattttaaa
     4141 taatagatta cctagtttta gagatataaa agaaaaatat catctaaaat ttgaaaagaa
     4201 aaaatacgtt ttgcaagtat ttcatccagt tgtaaccgaa ctagaaaata tagaaaaaca
     4261 aattttagct ttgatcaatt caatggaaca acaatctcaa ccaattattt gcattttacc
     4321 taatagtgat gctggtagcg aaattatcag aaaaatgtac cagaaatatt taaaaacgga
     4381 tattatttat attacgaatt tagagccgga ggaatacctg actatattaa aagagtgcaa
     4441 atttatgatc ggaaattctt cttctgggat aattgaagct gcatcattca aaataccagt
     4501 aataaattta ggaaccagac aaaatggacg agaacaatct aaaaatatta taaatgcaaa
     4561 tctaaatgaa aatgaaattg aaactgcttt gaataaaata caagatcctc agtttataaa
     4621 aagcttgaaa aatgttaaga acatctacgg tgacggcaac agttctagaa gaataacaaa
     4681 aacattgaat caaatattga atgatgggga agatattaag tggattcaaa aacaaatagc
     4741 ttattaaaat atactagtga tccatacatt attattgggg ctggcggaca cgcaaaagtc
     4801 attattgaaa ccttaagatt agaaaataag ttaatatatg gattttttga tgataagcaa
     4861 aataaaatgc aagatctgaa atatcttggg aaaataaacg aattgaatag ctgggcggat
     4921 agcaaaaaaa aaatctataa atttattgta gctattggtg actgtgtagt aagaaaaaag
     4981 attataaaaa aattgaatat gccaagtgag caatatggaa tagctgttca tccaagtgct
     5041 atagtggcaa aagaaatttc aattggttat ggaactgtta tttttgcgaa tgctgtaatt
     5101 aacccagatg ctgtaattgg agagcatgct ataataaaca caggtagcat tgttgagcat
     5161 gattgtagaa ttaataacta tgttcattta tctccaggtg tatgcttagc gggtggagta
     5221 catgtaggtg taggaactca aataggaatt ggttctcaat gtatccagat gaagaaaatt
     5281 ggatcttggt gtatgatagg cgcagggagc actattgtta aggatattcc ctcacattcc
     5341 ttagcttatg gcaatccagc taaaattaag aaagaaggaa ttaattttga attctagaat
     5401 tttattatca agtcctcata tgggaggaac agaagaaaaa tatgttgcta aggcttttaa
     5461 agataattgg atagcgccat taggtgaaaa tgtggataaa tttgaagaaa gtattacaaa
     5521 atatactggt tctaagggcg gagcttctgc gttaagttca ggtacagctg ccatacatct
     5581 tgccttaaga ctgcttaatg ttggaaaaaa cgatattgtc tttgctcctt catttacttt
     5641 tatagctaca gtaaatccta ttttatatca aggggctgta ccagtgttca ttgactcaga
     5701 acctaataca tggaatatga gccctgttgc actagagagc gcattagaag aagctgaaag
     5761 aaacaaccaa ttacctaagg caatcatcgt agtgaattta tatggtcaaa tggctgatta
     5821 tgaacgaatt ttagctattg caaaccaatt taatgttcca gtgatagaag atgcagcgga
     5881 atcgttaggt tctacttata agggaatttc gtcaggctta tttgggacaa ttgggatcta
     5941 ttcatttaac ggtaataaga tcatcacaac ctcaggtgga ggagctctag tgtctagtga
     6001 taaaaaatat gttgagcatt caagattttt agcaacacaa gcaagagatc aagcaccgta
     6061 ttatcaacat agcgaaatag gttataatta ccgtctaagc aatatttctg ccggtatcgg
     6121 acgtggacaa atggaagtat tgaatgatag agtagctaaa cgaagagaaa ttttcaatcg
     6181 atataaggaa gctttttctg gaattgatag aattgaaatg atgccagaag tggatggcat
     6241 ggtgccaaat cgttggttgt ctactttaac gatcaatgat tataattcta ttgatcaagt
     6301 agttaacgta ataaataaac taaatgaaaa aaacattgaa acaagagctt tgtggaaacc
     6361 actacatttg caaccagtat ttcaaggggt aaaaatgtat taccatgata ataatcttag
     6421 tgtgtcagaa aagttattta atagcggctt atgcttacct tctggaagta atatgacaga
     6481 tagtgaacaa gaatttgtta ttgatgagtt taaaaaaata ttcaagtagg tgtgatattt
     6541 tatgaacata ggaattattg gtttaggttc catggggaaa agaagactta gactcattca
     6601 agaacacttt aatgatattt tactatgtgg tgttgatact aatgaggcga gacgaaagga
     6661 agttgaaaag gaatttggaa taccaactta taatagtcta acagaggcaa aaaatttgtt
     6721 tgaaataaat tcagtttttg tttgtacatc ccctataacc catgaattta ttataaaaga
     6781 ggctttaaat ttagattgta atatctttac ggaaatcaat ttattaaatg aatattatga
     6841 tgaaataatc gaagtagcta aaaagaaaga tagattactt tatctatctt ctacattttt
     6901 gaagagaaaa gaaattcaat atattaagtc taaagtgacg cataataaaa aaatttctta
     6961 tcaataccat gtaggacagt atttacccga ctggcatcct tgggaagatt atcgcaattt
     7021 ttttgtttca aacaaaaaaa ctaatgggtg tagagaaata ttcgctattg aattaccttg
     7081 gattatagat acatttggga aagttaaatc gtttactgta atgacccaga aattatccaa
     7141 tcttgatatt gattattcgg acagtttcac tcttattatt gagcatgaaa ctggtatagt
     7201 tggaacttta agtgtagatg ttgtttcaag agtagctaaa agaaatttaa ctataattgg
     7261 tgaaaagatt ttaatagaat gggaaggtaa cccggattct ctattttcat ggaatgaaga
     7321 aacttctagt atgacagata ttcaattgta cgatgaggtt atgcatgata ataattattc
     7381 acatactatt attgaagatg catatttaga ggagattaaa gaatttttca cgcttttgga
     7441 aaagaaaatg gaaccccaat atacttttga aaaagataag tatattatta acttaattaa
     7501 cgacattgag gtaaattaat gatgaaagta attttttttg ggttagggtc aattggaaag
     7561 agacatctaa aaaatctaat aagctatgga aaaaagaatt ctattaattt tgatataaca
     7621 gcttatagta gcagtggaaa aaaagagttt gaagaaataa cgtatattca aaatagagac
     7681 gatttggata cacattatga tatagctttt attacaaatc cgacttttaa gcatctggaa
     7741 acactaaatg taattgttga aaaagttgat tacatatttt tagaaaaacc tgctttttct
     7801 gagagtattg atcttactca atataattat gattacaaaa atatttatgt tgctgctcca
     7861 ctgaggtata aaaatatcat ggaagttgtt cgtaatagta ttcaaagtaa aaaagtatat
     7921 tctgtaagag ctatttgttc tacttatctt cctgattgga gaaaaggtac tgattataga
     7981 aataattatt ctgcctttaa atctatgggg ggaggagtag aattagactg tatacatgaa
     8041 ttggattata ttataaacct atttggtttc cctaaatcag taaaaaaaca gtttggtaaa
     8101 tattcgaatc ttgaaattaa cagtaatgat ttagcaacat atatattgag atacgatgat
     8161 aaaattatag aaattcattt agattatttt ggaagagttc ctacaagaaa atttcaattg
     8221 tttactttag atgaatgtat caatgttgac ttattaaata ataaaataag ttttgaagaa
     8281 aacattgtta aagaaatgcc tgaatctgct aatgatatgt atgagaagga gttgaaatat
     8341 ttctttgaaa acgtagtaac aggcaaagaa aactggaatg atttatatca tgcaaacgaa
     8401 gtattgaaaa tagctgagga gaaataaatg gaaattttat ttactatttg tggtagagct
     8461 gggtctaagg ggtttaaaaa taaaaatttg aaatatttgg caggaaagcc cctagtccat
     8521 ttcgctttag caatgataga tatctatcaa caaaaacatc ctgaacataa ttgcacgact
     8581 tgtttgaata cggacagtaa tgagttagta gaattgacga ctaagttaaa tccgtctgtg
     8641 aaaattgtag aacgtaaacc tgaattagct acagatactg tagggaaaat tgaagtgata
     8701 cgtgattcat tcttacaact agaaagagaa gataatatat ttgatttaat tatagattta
     8761 gatttgactt cacctttacg tgaagtttct aatcttgaag aattaataga tgaacatcta
     8821 aaaaatttag ataaagacgt ctacttttca gttgtttctg caagacgtaa tccctatttt
     8881 aatatggtaa aaagaattga aaatgaagtt actatagtta ataaatcaag atacacatct
     8941 aggcaacaga cacctgaagt ttttgaaatg aatgcctcaa tgtattcttt taaacctgaa
     9001 tttctaaaaa aaagtaatta tatttttgat gggcaatgtg ggataataaa aatgagagat
     9061 tatctaattt tagatattga tagtgaagaa gattttaaat ggttagaatt tttgtttcct
     9121 aaatttttag aagattcttc ggagcttcaa gatatctata attatttata tcggaggtaa
     9181 aaggatgagc tgttatctta tcaccggtgg ggctggcttc attggttcta ccttagccaa
     9241 tcatttagga gaagaacatg tggtaatcgt cgtcgatgac ttatcaatgg gaaaagtaga
     9301 aaatcttgat atgacgaaaa atatcacttt tattgaggga gatgtcgctg agaaatcctt
     9361 gatggaagag atcatgaaag ctaataaatt tgattatatc tttcatttag cagcagttgc
     9421 tagtgtggca gattcagttg cccgaccagt agaaacacat cgtgtgaatt ttgaaagtgt
     9481 attaatgatt ttagaattga tccgcaagta tcaacctgat ttaaaacgga tcgtgtttag
     9541 ttcttcagcg gctgtttatg gggatgaacc gactttacca aaaaaagaag agtccgtgat
     9601 tcgcccattg acgccttatg cggttgataa attcgctgca gaacaatatg tgctagatta
     9661 ttgtcattta tatgatgtac ccggtagcgc agttcgtttt ttcaatgtct atggtccaaa
     9721 tcaaaatccg aattctccat acagtggtgt gatttctatt ctcgtagatc gctataagaa
     9781 acaaatcgta ggagaagcaa cttcatttac attatttggt gatggaagcc agtctagaga
     9841 ctttgtttat atagatgatg taatccaagc attactttta gttgcgaatg aggaagctgc
     9901 actggggaaa caattcaatg ttggaacggg taaagcaacc acacttttgg aattgattca
     9961 tgcaatcaat gaaattctcg gtgtagaatt gacacttgaa tacaaagaag agcgttcggg
    10021 tgatatccat gactctctag cagatattac taagatccag tcaattggct atcaaccgaa
    10081 atatggtatt ttaagtggaa tgactaatta tttgaaaact gaaatcaatt aaaacatact
    10141 aagataagat tagagggtaa attcaggctc tttgtcaata aggactgatg agttttgtaa
    10201 aatcaaatct gggataga
//
LOCUS       DANJMI010000083        10147 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000083 DANJMI010000000
VERSION     DANJMI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.085"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            290..559
                     /gene="rpsN"
                     /locus_tag="PWU81_002745"
     CDS             290..559
                     /gene="rpsN"
                     /locus_tag="PWU81_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL1111378.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            574..753
                     /gene="rpmF"
                     /locus_tag="PWU81_002746"
     CDS             574..753
                     /gene="rpmF"
                     /locus_tag="PWU81_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL1111379.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            785..1162
                     /locus_tag="PWU81_002747"
     CDS             785..1162
                     /locus_tag="PWU81_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111380.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1183..1332
                     /gene="rpmG"
                     /locus_tag="PWU81_002748"
     CDS             1183..1332
                     /gene="rpmG"
                     /locus_tag="PWU81_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL1111381.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1356..2339
                     /locus_tag="PWU81_002749"
     CDS             1356..2339
                     /locus_tag="PWU81_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL1111382.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2369..2491
                     /locus_tag="PWU81_002750"
     CDS             2369..2491
                     /locus_tag="PWU81_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL1111383.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2509..4032
                     /locus_tag="PWU81_002751"
     CDS             2509..4032
                     /locus_tag="PWU81_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL1111384.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4056..4295
                     /locus_tag="PWU81_002752"
     CDS             4056..4295
                     /locus_tag="PWU81_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1111385.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4488..4949)
                     /locus_tag="PWU81_002753"
     CDS             complement(4488..4949)
                     /locus_tag="PWU81_002753"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL1111386.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5144..5362)
                     /locus_tag="PWU81_002754"
     CDS             complement(5144..5362)
                     /locus_tag="PWU81_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1111387.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5480..7096
                     /locus_tag="PWU81_002755"
     CDS             5480..7096
                     /locus_tag="PWU81_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL1111388.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7337..8677)
                     /locus_tag="PWU81_002756"
                     /pseudo
     CDS             complement(7337..8677)
                     /locus_tag="PWU81_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8953..9108)
                     /locus_tag="PWU81_002757"
     CDS             complement(8953..9108)
                     /locus_tag="PWU81_002757"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL1111389.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9404..9736)
                     /locus_tag="PWU81_002758"
     CDS             complement(9404..9736)
                     /locus_tag="PWU81_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1111390.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         3373 a         1610 c         2006 g         3158 t
ORIGIN      
        1 attgccgttt taagtagagg aaaagctact taaaacggta tttgtgtttg attggactaa
       61 taaaagtgaa ttcttatttt tcagagccta aactgcatat tcttttaaat gatttgatta
      121 tctctatgta ccgataaatt cgttttcaaa tagttatcta ttcatatgag ttttatttca
      181 tgttatttat tgttgacaag agcaactctt gttggtaaag tatcagtgtc aaaacgtaac
      241 gattacgatt tgaggtgtct aaatcaaaat aaaaggagta taaaggtata tggcaaaaaa
      301 gtcgaaaatt gctaaagcaa agaaacaaat ggcgatgatt gaaaagtatg cagataaacg
      361 tcaggaatta aaagccgcag gagatcgcac cgctctcgct aaattgccaa gagattcaaa
      421 tccgaatcgt ctgaggcttc gtgatcagac ggatggtcgt ccaagagggt atatgcgaaa
      481 gttcggtatg tcccgtatta agtttagaga acttgcccat caagggttga ttcctggagt
      541 aaaaaaagca agttggtaaa aggaggaaat atcatggcag taccagcaag aaaaacatct
      601 aaggcgaaga aacggttaag aagaactcat caaactgtag taaaaccaga aatttcattt
      661 gacgaagcga atggtgatta tagacgtagc catcatgtat ctttaaaagg gtactataat
      721 ggaaagaaag taattaaagg agcttcaaaa tagtttaata tataactttt aggagggaat
      781 caaaatggaa attatttacc cacctttagt tgaagaagga ttaaagtatt atcttgaaac
      841 gacccagcaa tcgctagata aatgcacttt ttatcgttcg atggtagaga gaggaattat
      901 tactgaaacg ggtttaccga cccaacaggc aattgaaaat ggattagtca aagattacta
      961 tgaggaccaa ggcttatcat ttgatgattt tttaagaatc tatccaattt ttgaagagta
     1021 tgacgaggag ttatttcaat gtatcgatgg ttattgggag atacctgttg atatgaaaga
     1081 aaatttagtt tcacaattgg aatctggcga gttaactttt gaagatgcac aacaaattca
     1141 agcatatctt gaagatcgat aagtattgaa ggaggaagat ttatgcgtca aaatattatt
     1201 ttggaatgtg tcgaaactgg agaacgtctg tatctcacca gcaaaaataa acggaataac
     1261 cctgaacggt tagaattaaa aaagtattcg ccaaaattgc gcagacgggc aatttttaaa
     1321 gaagtgaaat agctaagtgg taaaaaggtg acaaaatggg cataccagtt acaatcgttt
     1381 ctgggttttt aggggctgga aaaacaacgc taatcaatca agccttgcaa gtctcaccct
     1441 accccaggga agaaattatt ataattgaaa atgagtttgg tgaagttggg attgatcaca
     1501 aattattagt tcatagcgag gaacgggtgt tacaactaaa taatggatgt atgtgttgca
     1561 gtcttcgcag tgatttatta gcaatattga tttctttact ggaaacaata gaagaacatc
     1621 aacaaccaat ttcacaaatc atcattgaaa ctacaggtat agcagatccc caaccaatca
     1681 ttcaaacact tttaacagcg cctcaactaa gaggaagagt atatatcgat agtttattga
     1741 ctgttattga tagtgacagt tttgaactta ttgaaagaga accacaagcc cttaagcaac
     1801 ttgtcatggc agatcgcatt tttgtgtcta gaaaacaaaa cttgaattta tctgcactaa
     1861 aaaaattaca gagagaaatc aaaagtatta accctttatc cgatattcga atgttcacac
     1921 atagtgaaac gattcaaaaa aaggattttt ataatttgaa aaaatttaat catcccttag
     1981 tttttgaaag tgattcatca gaaaatcact ataatggcaa atactatcat caacaccatc
     2041 aagtacatga gtttaaatca attctcttaa cttcagatca agacataaaa aaagattttt
     2101 tactccaatg gattgactgg ctgattttaa ccaaccaagg gagcctgtat cgttttaaag
     2161 gattgattgc tttacaagag caggatttta tagttgccat gcaagaggta aacgaacagg
     2221 ttaattttca atacaccact cgacaaaaag accaaaccac tattattcta atcggaaaaa
     2281 tgttggatga agaaaggatc aaacagagtt ttgataaatt actggcggaa agcaaataat
     2341 gataagtagt gattgggggt aatttctaat ggagaaaacg gatatatcta gtgcatatcg
     2401 acgacttaaa agcccgaata ttaaaactag aaaaagggca ttaaaaatta tcaaagaaca
     2461 taaacgaaat aaaatgaaga aattagcata attaaggaag gaaaaagaat gaagaaaatc
     2521 agtgtaggac ttattgggat agcagctcta ggattattgg gagcttgctc cagcactaat
     2581 gatgcaaaag tatcgaatga taaagatgga aaattagaaa ttgtaacaac attctatcca
     2641 atgtatgatt tcaccaaaaa catcgtcggc gatgaagcga acgtggattt aatggttcca
     2701 gcgggatcag aacctcatga ctatgagccc tcagctaagg atatggcaaa agctcatgat
     2761 gctgatgttt ttgtatatca taacgaaaac atggaatcat gggtaccaaa agctaaagaa
     2821 agttggaaga aagctgggcc taatgtagta gaaggaacaa aagacatgat tttattgcca
     2881 ggtagtgaag aagaagatca tgatcacgga gaagaagatc accatcatga actagatcct
     2941 catacgtggg tttcacccaa aatggcaatt aaggaagttt caaatatcaa ggatcagtta
     3001 gttaaattgt atccgaaaaa agcaaaagta tttgagacaa atgctgaaaa gtatttgact
     3061 aaactgaaac ggttggatgc tgactatacc actagtctta aagaagcgaa acaaaaaagt
     3121 tttgtgacgc aacacgcagc ctttggatat ttagctttgg attatggatt gaaacaagtt
     3181 cctattgctg gactaagtcc tgaagaagag ccatcttctg gtcgattagc tgaattaaaa
     3241 gagtatgtta aaaaaaataa gatcaattat atttactttg aaaagaatgc taatgataaa
     3301 attgcaaaaa ctttggccaa cgaagctggg ataaaattgg aagtactgaa tcctcttgaa
     3361 agtcttacaa aagaacaaat ggataatggt gaagattatg tttcagtaat ggaagacaat
     3421 ctaaaagcgt tagaaaaaac aactatggtt gctggtgaag aggtagttcc agaaaaagaa
     3481 gcgaaggatg aaaagacagt tgctaatggg tactttaaag atgccgatgt gaaggatcgc
     3541 gaattgagtg attatacagg cgaatggcaa tctgtttatc cgttgttaaa agatggaatt
     3601 ttggatgaag ttttcgatta caaagcaaaa atcaataaag acatgacagc tgccgagtac
     3661 aaagattatt atactactgg ctataagaca gatattgata ctatcaacat taaagacaac
     3721 accattgatt ttgtagtcaa cggtgaacat catcaataca catacaaata tgttggatac
     3781 aaaattttga attatgaaaa aggaaaccgt ggtgttcgtt ttaattttga aactgatgat
     3841 gcaggtgctg gtcgttttaa atatattcaa tttagtgatc atggaattgc accaagtaag
     3901 gcagagcatt tccatatttt ctttggtgga gaaagtcaag aaaaactcta taatgaattg
     3961 cataattggc ctactttcta tccagcttca ttgtctaaac atgaaattgc tcaagaaatg
     4021 atggctcatt aaaattcaat ttttggagga aatacatggt aagaaaagta ttattgtcta
     4081 tggattccaa gaatattgat gcttatcggc gttatcgatt tgcaaagcaa atgagattaa
     4141 atgggagtac attagctgat attgctgcaa ctttacatgt tagtattgac acagttcata
     4201 gagtattaaa acgggatcct gattgttgtt ttatcaaggt caatactgga agaagtagac
     4261 gtttagcaaa acggcataat agcttggcca attaattgat aataccagag tagatcattt
     4321 ttcccttatt tggatttgat ctgcttttta ttatgtcttg aattgtcaaa tcaagcgcaa
     4381 cactttatca aagaagactt cataaggtgt tctccaatct aaacactttc taggtctgtt
     4441 attcagtttt tcaatcatct gatttatcgt tgagttatct accgtagcta tctctttcga
     4501 ttttggcaag tattctcgaa tgagtctatt cgtattttaa tttgttcatt gttgccaagg
     4561 gcatgaggat ctggaaaata aaagggcata ttattcaatg cttctgtaac tgcgaaatga
     4621 ttggagaatt cttttcctct atctggagtg atcgttttga ccgcctcatt tggaatactg
     4681 cttaaaagtt caatcatttt ctcgctcaca taagcggctt tctttctggg gaccttcttt
     4741 cctgctaaaa ggaagcatga ttttctgtct gtcaaagtca ctaaacaagt accaccagtt
     4801 ttgccagcta caatatcggc tttccaatga ccaaaatcac ttctatcatc tgcggatttt
     4861 ggacggccat ggatcgaatg agtaaggcaa atctttccac gagactcgac atgatttttt
     4921 gtatgtcttg tttttcctcg atggtgcaat gaacggatca ggcctttatt gccgtgtgag
     4981 aggggttctg tatcgaaaag atcattgcaa attgcacgtt agatcgtgat aaaactaatt
     5041 gaaaagtcca tatgttcatg cttcaaacga ttcgagattt cctctggcga ccactgaaaa
     5101 tcgacaaata aatgacgaac aattgaccaa actcacggat catttagaat gtgataacgt
     5161 ccgcattttt ttcttcgata agcataattt ttttccgctt tggaaggtga atagtttttt
     5221 tcttgtttct tctcagttca tgtaaaatag taaacgggct atgctgaatg tcgttgccaa
     5281 ttatactaat agaaaatcct tcttcgtgaa gcagaaagat tctttctcgt tcgagtatag
     5341 taagatgacg atactggctc ataattattt tccttctagg agtgttctcg acaaacttat
     5401 actagttgga tatcgattat gactcatttt tatgtgttgc acttaaagtg taaattcaag
     5461 gtatagaaag aggttcccta tgaaaaaaat acctattaca atcttaactg gctatcttgg
     5521 ttcaggaaaa acgatacttc taaatcatat tctgtacggt gatcacggaa agaaaattgc
     5581 tgtcattgta aatgacatag gggaagtaaa tgttgatggg gtattagtcg acaagaaagg
     5641 ttttaaaaga actggagaaa aacttgtttc gatgccgaat ggatgcattt gttgtacatt
     5701 acgcgaagat ttgattgttg aactagatca attagtgcaa ttttctgact tgaatcaaat
     5761 tgttattgaa gcttcaggaa tcagcaaacc aattccaatt attcaagcaa ttatattagc
     5821 taaaaaccct ttagacattg atttgaccca acgattagag attggtgcca ttattatagt
     5881 agtggatgta aaacgttttt gggataattt cgaaacgggt gaaaagattc aggaaagaaa
     5941 agctgaaggt aatgtagttg aagaagaaca ggatattcgt ggcttgttaa ttgatcaatt
     6001 cgagtgttgt aacatattac ttttaaataa tagtgactta gtgtcaacca agacgattta
     6061 caaaattaac ggtctaattg ctaagttaca gtccaatgcc aaaataatta ggacaattaa
     6121 aggtgaagta gacttatcga aaattctaaa tacaaatctt tttaatatag atgagacttc
     6181 ctattcagtg ggctggttca aagagttaga gcttggctat caaaatcata ttcctgaaac
     6241 agaagaatat ggtatctcaa gttttatcta tcgaaatcgg ttactccttg acgcacaaaa
     6301 atttgcccat cttacggata aaaattttcc tggcaatatt acaagagcaa aagggtttct
     6361 ttggtttgcc caacatagtg atttttcgac gatctttgaa acatcgggta gctataaaag
     6421 tattgaatca ttaaattatt gggtagctag tttacctaaa attgagtgtc aaatgattat
     6481 acaaaaaaat cccgaattga aggaagcctg gcatccgttg tttggtgaac gcattaataa
     6541 actagttttt attggtatga agatggatca acaatgtatt aaggaacaac ttgagggttg
     6601 tttaactgat tctcttaatg cagataattt ccgaaaaatt tcaccatttc catggaaaaa
     6661 gtctaatatg tttagccaga tatcgttaat gaaattttta agaaaagctg gtgtcaagat
     6721 aggcgatgtc ataattattg gcattttgat tattacctct tttattccct tatttgtatt
     6781 ctctatagaa aaggagaatg aaacaactga agatgctatc aaatatgcca tcgttcgaat
     6841 tgatggcaag gaaatggatc gcttcgattt ggataagatc aatcataaac tcgtgaatta
     6901 tcatcctgcg aagggacaat ataatattgt tgagattaaa gatggaagaa ttcgagttaa
     6961 agaagataat agtcctgatc aaatcgcggt tagaactgga tggatctcaa aacccggtca
     7021 aacgagtatt tgtttacccc ataagttagt tattagtatt gagaaaaaag aaagcacaga
     7081 ttactacatt tactaatctg gaaatagatg aggaggtttt taataagaaa aagatattat
     7141 tattaataat agcaggggct ttattaagtg tgttaagcgc ttgtggacaa gaaaaagaag
     7201 cggtgacaac aggcacaagt atctttaacg ggacctgcaa tggtttagtt aacagtcggt
     7261 tagattactt attttggctc ttcgtcaatg agtgttggtg aagaaattca atagaataat
     7321 cgagatactc acacggctag acagctatct tgtctagccg tgttgttgtt ctggctttag
     7381 tcagttgggt agtattctta gttttcttaa aatattttcc tcgatataaa aagattcgtt
     7441 ctcggtagtt ttgaaagttt ctatagccat aggcaattcg ttttagtact ttgatatggt
     7501 tattcaaaca ttctataggg ccgttggaat agggaacgcg caacgctcgt ttgatttctg
     7561 tttggtattt cttgaaggtc gtgaacactg gttggtagtg ctctggcaac tccttgtagt
     7621 cttcttttag gagcgcgacg aattcaggaa catcttgtct tctgataacg gaaagaaaat
     7681 cctggtagac ttcgtagcct cgctttaagg aatcatcata agataatagc cgatcgacta
     7741 tctctgcttc tgtcagatga tctcgaaatg acggacgcca gtagcgatgc tgacctttca
     7801 attcccacgc attcttttga agtagtttcc agtatttctt taagtgcttc gaccgacgat
     7861 cccctttttt aaaggtcttc attacttgaa ttcggtggtt ggtaaaagcc cgaccaatgt
     7921 gttggctaat atgaaagcga tcggcgacga ttcgggcatt cggaaaacac tctttcacca
     7981 atgtgcgata gggtgtatag aaatctgaaa ccactagacg cacttgttca cggactttta
     8041 tagggtagcg caagaaatag tcgcgtaaag gattgagttg ccgattttcc acaatatcaa
     8101 tcagttgatg ggtcttgccg tccatcatga caaagctcat ggctcccgag acatttttca
     8161 ctgatttgaa ttcatcgaaa cacaccactt ctggtaacag ctggctcaaa gaaatggttt
     8221 taggccgaag gctgcgaagt attcgaatga cagtggtcgg agaaatgtgc ttcatacgtg
     8281 cgatcaaaga catagaaaga ggttcgctta atagctcaag aatggcttgt ttgactctgc
     8341 gagcaatgga gcagcgacga tcactaactg aatcttctgc taaaaaagtc tttccacagt
     8401 gtttacattt gaagcgctgt ttgttaatcc gcagaatggt cttgtagtta ctgacatcat
     8461 tcaataagat ggatactttt ttccagccct atttaatgat cgtttgttca ttttgatagt
     8521 ggcgatgaaa gcattcctcc ggttgatagg tcaatgtgcc atcaaaaaca aaacatgttt
     8581 cgtcattgat tcgctctttg cgaaaggcat cttcatgaaa gataagattt aggtctagaa
     8641 tatctagtaa ctctttggta aactctgaat aggacatctt gaaacactcc tttgaatggt
     8701 tttcgtcgac tttattctaa aggattttaa ggtgtctgtt tgcgtttatt agaaaaaaga
     8761 aaaagtattg gcaacagaag gcgtcaccaa cactagatat tatagatccc ttattttctt
     8821 cgcttcaaat tgtcaaggag tcatcataaa ttttgtatgg attcttttgt ttgggtagta
     8881 gtcttctaaa taatcttgga ttaacacctt catctctatt ttacttttat atttgttgga
     8941 gtcgattaat tctcatttga ttgatcgatg aaacgcttca attactgcat tgtcaacagg
     9001 agtcccagtt tggctcactg aatgtgtgat gtggtggtcg ttcaacagct cttgataaat
     9061 aaaagaccga tgctggctac ctctatcgga atgaattacc gtaggcaatt ctccttaata
     9121 aatttgtaat ggacggacga caatctcttt tttcatgtgt gaatctacca tatagtaacg
     9181 tagaatacga gttgctaagt caatataggt ggacaaatag aggcgaccgt cattacatgg
     9241 aatgtaagta atatccatca cccaaacagc atcaatttgg ttctgttcaa attatcgatt
     9301 caacattttt ttaccatttc tttgattgtt ttttctctac catgagaaga tttgcgtcga
     9361 tgaaatcctc ttgtgcataa attcatttca cgcataagaa cagctacaac acgtttattg
     9421 atttcgattc cttcatcgta aagtttttga gtgatccctg gagatccgta tcgatctttg
     9481 tgttcgtaga acactcgtct tcctctttta gtcgtttatt ttcctgttca agaatgtaga
     9541 aatctgcttt atcaggtaat ctttttcctt gtccagcaaa agcatttttc ccgtatcgac
     9601 gatattctga aacccatcgg gaaattgttg gacgagagat attgtattct tcagcaattt
     9661 tcttggtagt tacttttttc tcaaggatat cctgtgaaat ctttagttta aatttacgat
     9721 catatgtttt tctcaataga gttcattcct tccggtaacg taactgatag tcaagaaaag
     9781 tgtatcatag cccaacttag tatcagggtt tgtcaactaa actgtggaag ttgatagata
     9841 agagttttta acaaccaaaa ttctcttgac tatttttgaa atcagacaaa ttttgattgg
     9901 acacatagtt gaataaatct accgtcgtta cggaaggtaa atcgcatgct tttcaattct
     9961 agaggagaag tcctcgttga agtaccatag cccagttctg gattcgacct cctgcccact
    10021 tcatacgtaa ataaagtaat tttaggagtg agactgtgac aaaagttatt tttaagctaa
    10081 aaacgaatta aaaaacgttg ataattaaaa tcattcgttt tagattctat gaatcattat
    10141 agaacgc
//
LOCUS       DANJMI010000084         9945 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000084 DANJMI010000000
VERSION     DANJMI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.086"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            32..388
                     /locus_tag="PWU81_002759"
     CDS             32..388
                     /locus_tag="PWU81_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich SFCGS family protein"
                     /protein_id="HDL1111391.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            404..754
                     /locus_tag="PWU81_002760"
     CDS             404..754
                     /locus_tag="PWU81_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HDL1111392.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            783..1562
                     /locus_tag="PWU81_002761"
     CDS             783..1562
                     /locus_tag="PWU81_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HDL1111393.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1573..2235
                     /locus_tag="PWU81_002762"
     CDS             1573..2235
                     /locus_tag="PWU81_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HDL1111394.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAIFPA
                     VN"
     gene            2254..3360
                     /locus_tag="PWU81_002763"
     CDS             2254..3360
                     /locus_tag="PWU81_002763"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HDL1111395.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3350..4441
                     /locus_tag="PWU81_002764"
     CDS             3350..4441
                     /locus_tag="PWU81_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HDL1111396.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4454..5194
                     /locus_tag="PWU81_002765"
     CDS             4454..5194
                     /locus_tag="PWU81_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HDL1111397.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5482..7422
                     /locus_tag="PWU81_002766"
     CDS             5482..7422
                     /locus_tag="PWU81_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDL1111398.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7509..9431
                     /locus_tag="PWU81_002767"
     CDS             7509..9431
                     /locus_tag="PWU81_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDL1111399.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(9711..>9945)
                     /locus_tag="PWU81_002768"
     CDS             complement(9711..>9945)
                     /locus_tag="PWU81_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HDL1111400.1"
                     /translation="DLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQ
                     AVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
BASE COUNT         3248 a         1651 c         2207 g         2839 t
ORIGIN      
        1 acaaatgata ttttacgagg aggaaacaat catgattaaa gtagttattg cagaccgtat
       61 gggtaaagga caaaatgtgg ccaaaggggt agaagcagct ggtggaaaag cagttgtcgt
      121 accaggaatg ggggcagata tgcgtttggg cgatgtgatg caacaagagc atgcagatat
      181 gggtatctct ttttgtggta gtggtggtgc aggtgcatta acagcagcaa caaaatatgg
      241 ctatccagaa cgacatggta tgcgttcgat tgaagaaggg atcacagcta tcaaggatgg
      301 caaaactgtt ctaggtttcg gctttatgga tcaagaggaa ttagggaaac gtctgacaga
      361 agaatttatc aaaaagcatg gaaaatagaa agagggacat gaaatgagcg ttcaaaagac
      421 aattcagcaa aaagttatcg tctccggtaa aggggaaaca aagcaacatg cttttgcatc
      481 agcgttagca gaaattcaaa acaaaataat ggaacaatca aacgtattat tgcgaatcga
      541 accagtcaat gtggatattt taaaggcggt cgaaaacaaa tacacagaac gctttctatt
      601 tttcttcttc ccacggcaac gtattagcta tgaagtcgaa ctctctgtta ctgtggctgt
      661 aacagaaata aaattggcag acatatcgtt cagccagcaa atgactgaag atccaaatgg
      721 gattgccatt ccatttgtaa ctaaaaaaat ctaattttga atcataaggg ggaaagaata
      781 acgtggattt cgtaatcatt ctagtcaaat cggtcattat cgggggatta cttggttttg
      841 cagcatctgt cggggcagct cgaatgtttc atgcacctaa tacacaaggt ttaggagctt
      901 ttagaacatt aggcgaaatg aatgcctgca tgggtgatcc agcttctcat ttttcatttg
      961 gtttaggctt tttctttaat gcatgggctt cttcagttgg tgctggtgca ttcacacaag
     1021 atgtgacgca tcgaatcatt cccaattggg cagcggcagc acttctagca aaaaacaaag
     1081 atgtcaccca gaccatgcat gatccgaaaa aaatggggat cattggagcg gttatcgggg
     1141 cagtggtggt agccttctta aacacaacag ctgcagcaat tccagaggcg ttacaagtga
     1201 cggcggtttc cgttttggta ccagcagcga cgattctgat caatacagta atgccagtta
     1261 ttttttggtt ggcagcttta gacgcaggga aacgaactgg tttttggggc actctttttg
     1321 gtggtatcgc gcaattgatt atgggaaatg cggtacctgg tgttgttcta ggaatcttga
     1381 ttggtaaagg cgtcgatgag agcggttgga atcgcgtcac gaagagcatg atgatcgcta
     1441 ttgttttatt atttattttc agtggatttt tccgtggatt tgatatggaa atgatcaaga
     1501 gcttccagct tcaagtgcct aattggcttg ataacttgca caacatcttt agtgtgaaat
     1561 aggaggagaa tcatggagac aacagaagaa caagcaaaag tattagaagc aaagcggcaa
     1621 aagagctttt ggtttgcaga ctggtctttt ccgctaatcg taggaattat gtccgctgct
     1681 gtatttgcag gaacacacat gtatgtgatg tatggtgtgg gtgcattcaa tgaagtttcg
     1741 atcgtggcga tgttaaaagc cgggctggat ggcggatcct atggcgcagc ggcagcgttt
     1801 ggtgctagtt tcttatttgc cagaatttta gaaggctctt tagttgggat cttggactta
     1861 ggcggttcga tcctaactgg gatcggaatt ggggtaccag caattctctt aagtatgaaa
     1921 atagcggcac caatcgataa ttttgcactc tctttattga ctggtttggt tctaggtttg
     1981 attgttggtg gcatcattgt cttgattcgt aaatttacga tcaatcaatc aaattccact
     2041 tttggtgctg acgtcatgat gggggcagga aattcatctg gacgtttttt gggtccatta
     2101 attatattaa gcgcgtgtgc agcatctatt ccaatcggca ttggcgctac attaggagca
     2161 ctaggattct atgcttggaa aaaaccaatt gctggaggtg ctattttagg agcaatcttc
     2221 ccggcagtaa attaatagaa agagggtcat taaatgtttg atactttgat taaaaatgga
     2281 aggttagttg atggatctaa aatagaggtc gctatagaaa aaggcatgat taaagcagtt
     2341 gcagcagaaa taaatcagcc ggcgcaagaa gtagttgact tggagggaaa gtattattta
     2401 tccgctgggt ggatcgatga tcatgtccac tgctatgaaa agatgaatct ttattatgac
     2461 tatcctgatc agataggggt cgaaaaaggt gtgacgacgg tcattgatgc tggaactacg
     2521 ggagcagaaa acatccgtga attttatcaa ttgacgaaat cggtcaagac aaatgtgtat
     2581 gcgctaatga atatctctaa atggggcatt gtcaaacaag atgaacttgc tgatctaaca
     2641 aagattcaag aagatctagt aagtactgcg ttagcagaac tgccggaatt tattgtcggg
     2701 attaaagcga gaatgagtaa gacagttgtt ggtgaaaatg gcattaagcc attagaatta
     2761 gctaaaaaaa tccagacaga aaacaatgat ctgccattga tggttcatat cggttctgca
     2821 cctcctgagt tagaagaagt attgtcaaga atggaaagag gcgatgtatt gacccattgt
     2881 tttaatggaa aaccaaacgg tattttagat ccaaagacag atcaaattaa aaactttgtc
     2941 tgggatgctt acaataatgg tgtcgtcttt gatattggac atgggacgga tagctttaat
     3001 ttccatgtgg cagaaaccgc actaaaagca ggaatgaaag ctacctctat tagtacggat
     3061 atctacatcc gcaatcgtac aaatggccca gtttttgatc tagcaactac tttagaaaaa
     3121 ttgagggtag tcggctattc ttgggaagaa atcattgaaa aagttacagc agttcctgca
     3181 cggaattttc atttgaagaa taagggccag ttagctgtca attatgatgc agatttgacg
     3241 atatttgaga ttgttgaaaa tgaaaaggaa ttgaaagatt ccaacggatt tacacgtata
     3301 gctaaagaac aaattttacc gattaaaaca attattggag gcgtcatata tgacaattag
     3361 ctatggaaag tttgatttaa aagaagtcat taatgcatcg ggaaaaatga cgatcctagg
     3421 ggtttctaag gtatctgaaa gtgtcctagc agcacaacga tttggaggcg agcatttttt
     3481 tgagatgagt gatcttgcca tcaaaaccgg cgcttatctt tctcaattac tgaaagtgga
     3541 agatgcgcaa atcgtttctt ctgcttcagc agggattgct caatctgttg ctgctttaat
     3601 cggacagggc gacatgtacc atgtctatca tccctataca gaaaaaatcg ctagacggga
     3661 aatcatcata cctaagggac ataatgtaga ttatgggaca ccggttgaag taatggttgc
     3721 tcaaggtgga ggaaaagtca tagaagctgg ctatgcaaat atgtgtacac cggaacatgt
     3781 ttccatgatg attacggaaa aaactgctgc gcttctatat attaaaagtc atcatgcagt
     3841 tcagaaaagt atgctgacgg tggcagaaat ggtagaagtt gccaaagctc atcgtttgcc
     3901 attgatcgtt gatgcagctg cggaagaaga tttattcaaa tatagcgaaa tgggcgttga
     3961 cttagtgatt tactctgggg caaaagcaat cgaaggtccg agtgccggac tggtcatagg
     4021 aaaaaagaag tatattcaat ggattcggct acagtctaaa ggtattggcc gagcaatgaa
     4081 aatcggtaaa gataatattt taggctttac ccaggcacta gaagattatt taaaaaatgg
     4141 gagcgaatcc ggtgactcca tgaagcaacg attagcgcct tttgttaacg caatcaatca
     4201 acttcctgac ctttcagcga aaatagttca agatggtgcc ggcagagata tttatcgagc
     4261 aagtgtaacg attaatggcg ataaaagtgc gaaagaagtc attcaggaat taaaagcacg
     4321 aaatccagca gtctacaccc gagaatatca agcaaataac ggaatcattg agttcgacat
     4381 tcgttccgtc agccaaaacg aaatggaaaa aattataaaa agattgagag aaatcatgta
     4441 ggaggctttt aaattgtcag ttatcccaaa ttattataaa aatcgtgtat gtttaaatgt
     4501 attagctcat tcagttgaaa atgcaaaagc atgctatgaa gcagcggaag ggcatgtcgt
     4561 actaggtgtg ctatcgaaaa attatgctag cgatgaagca gcaatcgaag atatgaaaag
     4621 gtaccaagac gcaaccaata atgcgttgtc agttggatta ggcgctggag atccaaacca
     4681 aagtcagatg gtttcgcgtt tatctggggt tttacaacca caacatgtca atcaagtatt
     4741 tactggcgtg gggacttctc gtgctttatt gggccaaaat gatacagtca tcaatggatt
     4801 ggtttcacca acaggtaaga tcggctatgt caatattgct acaggtccgt taagttcaca
     4861 agcgccagca gctgaagtgc ctattgaaac tgccattcaa atgctgcaag acatgggagg
     4921 tacttcaatc aagtatttcc cgatgaaagg cttagcacat agggaagaat ttaaagcagt
     4981 tgcggaagct tgcgcaaaat atgaattcta tttagagcca acaggaggaa ttgatctttc
     5041 aaactttgaa gaaattctga aaattgcaat cgatgcaggt gtaaaaaaag tgattcctca
     5101 tgtttatagt tcaattatag ataaagaaac aggagatacg cgtataggag acgtaaagat
     5161 actattaagc atggtaaaaa aaattattca ctaactcatt agaaaataag aagcttattc
     5221 cagaatgaaa ttaaatggaa taagcttttt ataattaatt acggaaattg ttaatgtaga
     5281 taataattta gatgtacgta agcagaagta cagaaatgta agattttcca atattatttt
     5341 attcaacata ataaatcgta tatcaatttt tttagcgaaa tttctattta ttccaagaat
     5401 ttcagttatt acggaatcat tttgtgctta ataaaaagaa accgttttta tatactataa
     5461 atgtagaaag gcggtaggaa aatgaaaaaa attattgcaa tcacatcctg tccagttggt
     5521 attgcccata catacatggc agcagaaaac ttagagaaag cggggaaagc agttggcgcc
     5581 gaggtaaaag tcgaaacaca tggttccatc ggtattgaaa atgaattgac tgcaagagat
     5641 atcgaagaag cagctggtgt aatcatcgca gcagatacta aaatagataa atcacgattc
     5701 ggcggtaaac ctttgataac cgtaggtgtg caagaaggaa tccaccatgc agatgagttg
     5761 gtgagagata ttttagcagg aaaagcgcca gtctataaaa gtacggaggc aattatttct
     5821 tccgaaaata aaagtgaatc agaaaattta ggtaaaaaaa tctataagag tttgatgaat
     5881 ggcgtttcgt acatggtacc tttcgtagtt acaggagggt tattgatcgc gatttcttta
     5941 acattaggag gaacagctac acctgaaggg atcaagatac cagaaggaac gatttgggca
     6001 acgatgaata gtatcggaag tatcgctatg gggttgatgg ttccaattct atctgcattt
     6061 attgcgcaaa gtattgcaga tcgtccagga cttgttcctg gatttgttgg tgggatgtta
     6121 gcggcaaacg gagcattata tggtagtgat gcgaatgcgg gattcttagg cgggatcatc
     6181 actggatttt tagcaggatt cattgcttta ggaatcaaga aagtgcgagt accgaaagct
     6241 ttacaatcga tcatgccaat tattgttatc ccaattttag gatcattggc agttggtttt
     6301 gtgtttatct atgttattgg agaaccagta gctcttctat ttagttcatt gactcacttt
     6361 ttagcaggga tgcaaggagg aagtgaaatt gttctagcta tgatcttagg agcgatgatt
     6421 gcttttgata tgggggggcc gttcaataaa gtagcctttt tgttcggatc tggcttgatt
     6481 gcagaaggca attattccat tatggggcca attgctgtag ctatctgtat ccctccatta
     6541 gcattggggc tggcatcatt gattttaaaa accaaattta cgaaaacaga acgagaagct
     6601 ggtaaggcgt ccttagcaat gggactgttt gggattacag aaggcgctat tccgtttgct
     6661 tcaacagatc cactacgggt gattccaagc attgtaatcg gggcaatgac aggaagtgtg
     6721 atcgcgatgc tttcaggagt gacagatcat gtagctcatg gcggtccgat cgtagcggtt
     6781 ttaggagcag tggatcacgt agccatgttt tttgtagcag tgattgctgg tattgctgta
     6841 accgtgctaa tgttaagaat ccttaaaccg acattacaac aaactgcggt tgctgcagca
     6901 aatggtgtga cagtagcaga tctatcagag actgaaacaa gtgatgttta tacgaaaact
     6961 gaaacttttg attctatcac ggatattatg tcagatgaag caatcgtttt aggtgaaaca
     7021 gcaacaacaa aagacggagt aatcgaagat ttagtccaac gtttagaaaa aacagcggcg
     7081 atcacagata aaaccagatt taaagaagtc atctatgata gagaacatga aagtacaacg
     7141 ggaatcggta tggggattgc tattccacat ggaaaatcag cttatgttct taaaccaaca
     7201 gtggcatttg ccagaagtca atctggggta gattggcatt ctttagatgg aaaacctgca
     7261 catatgatct ttatgattgc tgtacctgaa aacagccaag gtgatatgca cttgaaaata
     7321 ctgcaacgtc tatcacgtaa attgatggat gatgacttcc gtcaagcact gatggaagcg
     7381 ccagataaaa caacagttca ccaattatta agcgaaatgt aacataagat gaaaaatcga
     7441 ccgtgatgag acataataga tgtgttatca tgatcgattt tttatttaga agggagtttc
     7501 tgcacattat gaatcagcgt cagcaatctt tgatctatca attaagtgag aacaataatt
     7561 atataacaac aaaaaagcta gcagagcatt tccatgtgtc agaaagaacg attcatagcg
     7621 atttgaatac cattgaaact tggttgagag acaaacatct gcctttaatg tttgaaagaa
     7681 aaaaaggtac aggtatcttg ttgaatggtc tgcctcacga aagggaacaa ctgaaacgag
     7741 cgcttaatga agagactcaa acagaaatgg ataaaaaaca gcttcgtcag ttattgattt
     7801 tccatctgct agtagcaaaa gacggattta gtcttgaaga actttcagaa aaactatatg
     7861 ttggaaaacg tacgattcgt aaagaattga cagagttgca aagctttttt gagtatcacc
     7921 atttacagct agtatccaaa acaaaactag gaacttttct taaaggcgat gaacaagaaa
     7981 aaagacagtt gcttgttaag acattacgaa atatgcagaa agaagatcca caaagtccgt
     8041 cgttgaaaga attcttcgct aaagatacat taaaagttat ccaacataca ttacaagatg
     8101 ttttttatga aaaccattta gaacagccga atggactggc tagtgtggag attcacatct
     8161 actttatgct cgagcgaatg aaacagtacc agaaagtgaa attaagtaaa gacgaaaacg
     8221 aagtcgtgga acacacccaa gcacaaaagt taagtagtca aatattagca aaactagcca
     8281 ccatctatcc aattgaattt tctcctgatg aaatcaatta tctggctttg cgtatcgcca
     8341 atctttttac aaattcacaa gcagccacac gttttcaaaa ggaatctagc gcacttgctg
     8401 atcatttgat cgaacaagtg gaacagtttc taggctattc attaaaagaa gatcagctac
     8461 taaaacaaaa tttaaggtct catttatctt caacttattt tcgactccat tatggtttgc
     8521 agatttctaa tccattgacg aaaaatgtgt ttagtacgta cacacaactt ttcttagttt
     8581 tacagttaat tttagaagat tattttgaaa aggaaaattt ttatgttcca caagaggaga
     8641 cagcttattt aacgatccat ttccaagcag cgatcgagcg acacaaacat cagaaaagta
     8701 gaagatatca aacggtttta gttagtgaat attcaaaagc tatggctact tttctcgaag
     8761 cacggctgaa cagagaactt ccagaattaa cgattatgga tctagttgaa tataagccag
     8821 aattaaagag acaagatttt ccctctgttg attttatctt ggctacgtta ccatttaaac
     8881 atataagcat tccagtaatc gagatctcac caatgatcac agaaactgat ctagcctatt
     8941 tgacaaaata tatgctggag catgtgccta tcaagaagaa aaaaacattt gatctagcaa
     9001 gctttactca tccatttctg atctttcctc aacttgaacg gacagaccca gccgatattt
     9061 taaactttat gggaaacgtc ttagtggaac atcattatgt ggagccggaa ttcatcgatt
     9121 ctgtcttgga aagagacagt cactccagta ctagagtagc accatttgta acaatccctc
     9181 acggtaatcc gctgtatgtg aagcattcga tgatttctat tgctacaatg aaagaaccag
     9241 tcctatggca cggggaacag atacgtatcg tactgctatt agcaataaag aaaaatgatt
     9301 tgaaacatgc agagttcaaa aaaatatttt ctgttattca ctatctagaa aagcagccag
     9361 aacaaatggc tcagttgatg caatccaatc atccgctaga aatcttaacg ttgttatcta
     9421 gttatgaata agtagcgatt aaaaaactgc ctttatattt ataataattc tatctattta
     9481 tttggaagta gaaaactaaa tacaaaaaaa gaggatgtgg ccaaagttgt ggcacatcct
     9541 tttattttga gtttcttata attttttata gttgcttacg atgtcttgac tactggtaaa
     9601 cggtgttcaa gagacaattt gtcggcaata aggcacaatt ttgtaaaata tggaaagcta
     9661 tttttttaac attgtttctt agtgcttgaa aaatcatttt gaatacttct ttatacgatc
     9721 gatgtacgtt gatttaagtc taaaactttt cttttgattc cttctctgac ttttctgaat
     9781 tccgttaata tttcttcttc gctcccgaca gcttgtgcag gatcttttag gtcccagtga
     9841 aggtgttcgt gattgtttgg aacggcaggg cacttgtctt tggcatcacc acaaagtgta
     9901 atgatcaagt ctgcctgagt aaagtaatct ctatcaatca aatca
//
LOCUS       DANJMI010000085         9439 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000085 DANJMI010000000
VERSION     DANJMI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.088"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(<1..450)
                     /locus_tag="PWU81_002769"
     CDS             complement(<1..450)
                     /locus_tag="PWU81_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1111401.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQ"
     gene            651..1865
                     /locus_tag="PWU81_002770"
                     /pseudo
     CDS             651..1865
                     /locus_tag="PWU81_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2044..2373)
                     /locus_tag="PWU81_002771"
     CDS             complement(2044..2373)
                     /locus_tag="PWU81_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL1111402.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(2363..2635)
                     /locus_tag="PWU81_002772"
     CDS             complement(2363..2635)
                     /locus_tag="PWU81_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL1111403.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(3086..3889)
                     /locus_tag="PWU81_002773"
     CDS             complement(3086..3889)
                     /locus_tag="PWU81_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL1111404.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3867..4991)
                     /locus_tag="PWU81_002774"
     CDS             complement(3867..4991)
                     /locus_tag="PWU81_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1111405.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(5007..5822)
                     /locus_tag="PWU81_002775"
     CDS             complement(5007..5822)
                     /locus_tag="PWU81_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL1111406.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5812..6684)
                     /locus_tag="PWU81_002776"
     CDS             complement(5812..6684)
                     /locus_tag="PWU81_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL1111407.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6769..8058)
                     /locus_tag="PWU81_002777"
     CDS             complement(6769..8058)
                     /locus_tag="PWU81_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL1111408.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(8286..8930)
                     /locus_tag="PWU81_002778"
                     /pseudo
     CDS             complement(8286..8930)
                     /locus_tag="PWU81_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2911 a         1995 c         1520 g         3013 t
ORIGIN      
        1 ctggtaatgc ttcgttgctt cacgcaagat ccgtgtcacc gtcccatccg agacgaagta
       61 cttcttagcg attagtttac gtgattgatt ttcgtacagt tccatgagaa ttgcctgctt
      121 cagttccttt gttagttgat gatgttcatc aactaagtcc gttttcgcag aaaaggtcct
      181 tccacaatca tggcagagaa aacgggtacg ttttaattct aaaatagtta acttttcttt
      241 gactttcaat atctgagtgc gtgtcgtata ggaaccgttg cggataatac gaaccgatcg
      301 acagtgttca caataggaag gagagtcatc caattttcct ttaataaact gcgcttgaac
      361 gcctttatat tctttttcaa tcagccattc ttcatcaaaa aataaatgtt tatctgttaa
      421 tccgagtaat tttctagtat gattatccat tgagagactc ctttggtagg atgacgtttt
      481 ggtcgactaa attttaccaa aagtgagtct ctttttgagt tgaaacgaag aaaaagtgtt
      541 agtagaaaat cgccttcgat tttccaccaa cacaaaatat tatagaagca aaatcaattc
      601 taccttagat gttcttatat cactctatca gtcctatttg acaaagagcc aagaaaaaga
      661 cgttggtcgt cgatgctgtt ctctcgccta ctcctcgtgc ttgtagaagt tgtggttcta
      721 ctgtggtaga tggaaacggg aaagcaatta tagtgaaaaa tgggaagaag gaaacgattg
      781 tccgttttga acaatacaat catatgcctt tggttacgcg cctaaaaaag caacgctata
      841 cctgtaaaaa ctgtcgtacc cattggacgg ctcaaagtta ttttgtccga ctcagccatt
      901 caatcgcaga tcatgttcga tataaaattg cttctttact gactgaaaaa gtatctttgt
      961 cttttattgc gaaaagttgt caggtatctt tgactaccgt tactcgtaca ttgaaagagt
     1021 ttaaaagcta tttaccaaag ccttctaaga agattctccc cagagtattg atggttgatg
     1081 aatttcgttc gcacgcttcc atagaagata aaatgagctt tatttgcgca gatggtgaaa
     1141 caggaaactt aattgctgtc ttgcctacgc gtaaattacc tcgattaaca agctatttct
     1201 taggctgcac caatccagaa gaagtagaat tcttagtcac agacatgaac gccgcttact
     1261 tccagcttac caaacgtgtt ctgccaaatg cgaaagtagt gattgatcgg tttcatattg
     1321 tcaaacacat gaatcaggca ctcaatgagt tgcgtatccg tgaaatgaat gagcttcgta
     1381 aagcaggaca gaaaagtcag gcagaaaaac tgaaaaagaa ctggcgcttt ctgctaaaaa
     1441 atcgtgcaag catcaaccat tatgagtaca aaacatggaa aagtttccga gcaccaaaat
     1501 acccatttct cactgaagca atgatgattg atcgattgct tgagttttct gcgcctctaa
     1561 aggaggcgta tccctttttt catgaattag tcgaagcctt tcgagacaaa gaccctgact
     1621 tatttttctc cttattggca gaacttcccg aaacgttaga tgacggcttt cgggagaagc
     1681 ttcaaaacct tctgacctat gaagaaggta tcaccagcgc aatgatctat ccttattcca
     1741 atggaaaaat tgaagcgaag aatacccaca taaagacaat gaaacgagta tcctacggat
     1801 ttaaatcatt cgagaacatg agaattagaa tctttttgat caatcaatta atcaatgtaa
     1861 gataacaaaa aatctgaacc agaatgtggt tcattctgac ccggatttga ttttgcacaa
     1921 ctcatcagtc cttattgaca aagagccaaa aatctgagcc agaatgaggt tcattctgac
     1981 ccagatttga ttttgtacaa cccatcagtc cttattgaca aagagccctt ctttttagta
     2041 ttattataat tcttccatgc gttcttccag tcgctgtaaa tcgcttggac ttaattcccc
     2101 aatttttttc catttgacct ttttgttgtt tttgggtaat tcgtttagag ttcctacgtc
     2161 aatccaagat tcttttttta gtcctgcctg tttccaatcc cttatttcaa cgtatttcag
     2221 ctttatttta tcggatttag tatgatattt tgaagtaatt gacagaaacg aaattgtgtc
     2281 cagtccattt tttagaatat agacaggccg gcgtttaccg ccctctgcag ctgtgaaagc
     2341 aacgtagacg gttgctatat cattagtatt catcttcatt gaaccattct tcaatttttt
     2401 cgtcagtgtc tagtttttgg acaggcaatt tttccgctaa tctttgaata ttatggattc
     2461 gtttactttt ttccgacaat tcaatagaaa atgggatttt tccttgctca acaatttgct
     2521 tataaaaaac ggtgacggct gttttggggc tcatgcctag ttcatcaata atgctttctg
     2581 cttcattttt caatttttcg tccatagaaa tttgtaactt agccattttc tccacctctt
     2641 tctgacaaac acattatatc acggaaagag ttcaaatgga attaatacaa ctacttttga
     2701 actccttgaa atgcagtgta tttatctaaa tataaggaaa ggatatagtg ctaaatggcc
     2761 ttgatagata gaaaagtttc aaaatccgcc acagcgataa ttgaagcgaa cactcaacga
     2821 cacacagaac aggaaaagac tcctaaaaga accaaattaa ctaggacgtg tctgaaaact
     2881 ccacaaagat gtggtatgct ctggtaaaaa acgaatggca caaccacgaa gaccgatatg
     2941 gttcttggaa aactttctcc accctttttg taattggagg gatagtggtt tattgatagc
     3001 gattttccaa gttcttcaag tggaaccaga cattgagcac cacggctaaa aagtgaatga
     3061 agtctttatt tttaatcaat aatttttaat gattaccagt aattggtttt ttcatgcgcc
     3121 ctatttctat ttgatgtgga gttaaataat gctgataagt aaaaattttt ccgtcaatta
     3181 aactgccact agtgaatcct gttctattgg taaaaagaga atagtctggg tattcatcta
     3241 caccaaatga taaagattca gccataaact gttttttacc agcaaattca gcactgctac
     3301 cttgatggat atgtcctaca aaaataccta aaatatcact ttcggcaatc aattcagcaa
     3361 aaccttccgg aacttcagtt acaatagatg gatcttccca cgacaatgga tgatgaacca
     3421 ataaaatcgt tcccttttca gcaggtgtac ccaaccactc ttgtaattga atcatctgtc
     3481 ctatcgatat ttttccttcg tgctgggtat cttgagcaga atccaaggaa ataattcgta
     3541 acccattttg attcgtacaa tggatatact ctcccgtttc attgctttca acactcattc
     3601 cttcaaagaa aacactgcga cgatcatgat tcccacgaca aaaatagtaa ggtgtggttg
     3661 ggaaatattg atccaacatt ttccgtaaga cttgataatc agaaatcccc ccttcatgta
     3721 ctaaatcacc tgttaataga ataaaatcgt acactgttaa cgagagttct tgtaacaatt
     3781 tttccaatgc caactgtgga tttttaattg ttggaaatag ttcagcagta aacaagttag
     3841 tttgatagtt ttctctaaaa tggatatcag agagatgcaa aaagcgcatg gctattccct
     3901 ctttcttcaa taaaataatc aggatatcct aaattttctt gcgtctgata atcaaaaaaa
     3961 tgcattcttt caggttctat gcggagattg actgcatcac caatctgcca agtaggtttt
     4021 tcattaagga caatcatttc tataccatcg actttaacgg tgtacgcata actttgacct
     4081 aatagctcac gatacttcac ttgaccgtgt aaatcgcttg tttcttttga aaaggttacg
     4141 tgttctggtc ttatgcctaa atacaatgag ccactaacct gaagaagttg tttttgccaa
     4201 gctggattca atgttaaaaa ttgactggct acctttaatg ttccttgatc ataagtaact
     4261 ggcataatat tcatgcctgg cgaaccaata aaatttgcag taaaagtatt tttaggtttc
     4321 gtataaacat ttttcggcgt atcaatcatc tgaattctac cttcattcaa taaaacaatc
     4381 cggtcagcta acgtcatcgc ttcaatttga tcatgagtga cgtagacaaa agtttgttta
     4441 taacgttgat gaatttttac caattctttt cgtgcatcca atcgtaaacg cacatccaaa
     4501 tttgataacg gttcatctaa taaaaaataa ttactccgtt taaccaccgc acgagccaat
     4561 gctacacgct gccgttgccc tcctgacaaa tctttaggaa gtcgatcttg gtatggcgtt
     4621 aaacctaaca tcttcaaagc ttcttccaaa cggtgctgaa tgtcagctgt cttaatttta
     4681 ttcgccctta atgcaaaaat aatattctcc tttacagtca tgtgaggata taaagcataa
     4741 ttttgaaaaa ccattgcgat ctctcgttct ccacaaggta gcttatttgc tagttgtgta
     4801 cctaaaaaaa gctctccact cgtaattttt tccaaaccag caatcattct cagcaaagta
     4861 gattttcctg aaccagatgg tcccagtaac acaattcgtt ctccagcggc cacagctaaa
     4921 tcaatttctt ttaaaatggt atgttctcca tatttttttc cgatatttct taattcaagc
     4981 gtttctccca tgatttgtcc tccaatttat ttaatacctg cagaaacaaa agtcccgata
     5041 atatattttt gaaaaattgc atacaagact aacggtacaa acattgtaat taaactaatc
     5101 gccattgcta cggtaaaatt tgtcccacct tcagaactaa tataggtctg catcgccaac
     5161 ggcaaagtat aattatctac tgtccgtaaa attaataccg gccaaacata ctcattccaa
     5221 gaggtaataa aaaaccaaat accggtagaa gtaatcgcat gtctagtaag aggaaaaaca
     5281 attttactca tgacggttgt ttgtttgatg ttatccaatg ccgccacatc tagaaggctc
     5341 actggaattt cccgcattgt ttgcgtcaat aaaaaaatgc ccatcgcatc agcaaattgt
     5401 ggtattatca caccccaagt agtatcaatt aaattaagtt ttgcaatcaa caaataatta
     5461 ggaatcatcg caacagtaaa aggaatgaac atcgtaccaa tgatgataaa gtaccagatt
     5521 ttcttccctt tgattcggca ataagtaaaa ctatatgccg caaaaaaagc tacgcctaac
     5581 ttaacaaccg ttataattgt agcaataaaa aaagtatttc tcgtaatttt gataattgga
     5641 agattttcga acaaatagca aaaattttcc aaggtaaacg gctgtggtat caaactagta
     5701 atcgtctgat aggcatcttt caatggctta aaagaattgg ccataataaa caataatgga
     5761 tagataataa tgattatgat gaacataaat aagagatgtg gcaaccagcg tttaattttc
     5821 atagtaaacc cccttttcca caaatttaaa tattacccct aagaaaacag caaatattaa
     5881 taaagttaaa actgaaattg ctgaagattc tccgacttta tacatttcaa aagccttttg
     5941 ataagcataa tagatcacgt ttgcactacc attattcggt ccaccacgag tgatgatctt
     6001 aatcggagta aacacatact gcagaccttg aacaatggtc atcaccaata aatatagtcc
     6061 tgtcgaacta ctcataggca acacaattgt aaaaaaaatg cgccagatgg gtacacgatc
     6121 caacttagct gcttcgatca tctcttgatc aattcccaca attgctgcaa aaatgacaat
     6181 gaaattataa ccaaaaactt tccaagcagt aatacctgca atcacagcaa ttacccaacc
     6241 ttgtaaattc atccacatag gcagtcttaa accaaaattt cccaacaaga cagcaattgg
     6301 tcccgatacc ggatttaata tccatgtaaa aaccatcccc gccactacca aagaaatcac
     6361 agctggaata aaaaataatg gtttatacaa attctgccct ttttctataa ctattcctaa
     6421 taaaaaggat gtaatatatg ggaacacaca gctcagcaag acaaatagaa cggtatagga
     6481 caaagtattc ttcaacactt tatgaaagac tggatcagtc agcaccttta catagttggc
     6541 aaagccaaca aattctttag tgggggccac catattccag tcaaaaaaac tcaaatagat
     6601 cgtataaatc aatggataaa aaacaaacac gcccaacaaa ataattgctg gcgccagata
     6661 caaataacct acatagcgct tcatttccga cagcctcctc atccttatgt caaaagcaga
     6721 cgaccttgtt tttcctttct tacaggcgct gtcgtctgct tgctttgctt atttgagtaa
     6781 ctcattaatt tccttttgag tagctttcat cgtttcattg acggaattac ctgataaaat
     6841 tttatcccgc atatccaaca atttttgttc agcttctaat ccagcatctc caggaaatga
     6901 actccaactg accatcttat ccatctgatc aattgctggt atcatcatct gattttcagc
     6961 taagaaagct tttaatccat tttgggcgtc tgcaacatct ttacgtggtg gtaaataacc
     7021 agtaccttct gtccatttag ctaaagattc aacactataa agatatttta agaaatccca
     7081 agttgcttcc tttttcttat ccgtttgtga tgtaacagct aacattgcgc caccagcagg
     7141 tagtttaact tttttccctt cccaacttgg tgactcaact gctttagcag taaaagtact
     7201 atttttttga atgttgtccc tttgagcaat agtggtatag accatcgcga cgttaccatc
     7261 aataaagctt tgcacacctt gttcccacgt agtgtgtaat gccgtctggt cttttaccat
     7321 cctttgatat aattcataag cagcgatacc atctttagaa gcaaaggtag ctttgccatc
     7381 ttttattaca cttgccccat tactttctaa aattgcttgt tgtgcccaat tatcagcagg
     7441 ttcttgaata tacacgccat actttccagt tttttctttt attatcttag aaaaagtttg
     7501 aacttcttcc caagtttttg gaccattttc atccaagccc gctgctttta atatatcttg
     7561 atttaaatat aaaaccggat tactaatgga gtaaggaata cccacttgtt taccatcttt
     7621 ttcagacagt gccatgatat tgtctaaaaa atgatcttta aaataatcag cattatccgg
     7681 atccacttct tctactaaag attgaggatt agtatatttg aaattttctg caaaataatc
     7741 tttaaaagcc catcccactt gtactacgtc cggtatttct ttggaggcaa cagaagcttg
     7801 taaattttgc atcaagcctt tatacatatc aggattatat tttgctacaa ccttcacttt
     7861 gtcttgagat tgattgtatt tatctactaa ttcattcact gtttttccac ctagggtatc
     7921 cgcattaaca tgccaatact ctatcgttac acgaccctcc gatttttttg ttgtcttggt
     7981 attcccacta ccacaagctg ccaaagagat gacacttaaa acagacacaa cccctataac
     8041 aagatttttc tttaacataa cattaacttc ctcctaattt ctttatggcc atttttctaa
     8101 tcaagaaata ttatgaacta ccaatgtaaa tttctagtta attttctgta aataattcga
     8161 aatttgtgca tttcggggta cagggtcact ggacatatcc atcaagctaa gaataaagcc
     8221 cccatatggt catgtatccc atctccaaat ctcttaactc ctctgttttg attataaaat
     8281 agtttattct gtgctccttt cttccataaa taggcccatg cacaacgtga ggttatatta
     8341 taaacaccac agcgcgcaag gtactcaatc gtttctagtt cattgactgt ccatacagaa
     8401 aatgtcaatc ctagctgctg ccccacttca cgggccaact tagttaacca tccttcatga
     8461 atattgacga tttgaacgct ttgataggct aatttctgta aggtatcgat caaatctgct
     8521 tcggtaatat ccccaagttt tttcccttcg aactgatttt caatattata aaatatatgc
     8581 tttccccaaa tcgcagccca agataattgt acatagccac taagaacaat ttgtggccag
     8641 atttgatagg tttgtgccag cgaaaaaacg gccatttcca atccctcttc ttttaaatca
     8701 cagttaatgg tcatggttgc cttctcattg gccactgctt gaaacacctg ctcaagggtt
     8761 accgaatctg ggacaacttc ttacctgtca tgggctagta ttagcgtacc atctgtttgc
     8821 tgacgcacat ctacttcaaa acaaagctgg cgatccaaac aaaagcgaag aaatgcccag
     8881 ctattctcct ccgtattgtc actaccagag tgtgcagtga tcatggtcat tttgacccct
     8941 cctttaattc cgtgattttc tcccacacgt ccactatacc gatgaagcgt taatttcttg
     9001 ttaatttctt gtaaattccc tgcgtttttt cagtaaacta gttgtaaatc ttgcatgctc
     9061 cttcctacga actcatgaat cgtcgatttt gcccttctag ctagtttcac caattaagta
     9121 gttctacatt ccccgctttt aacctactac accttttttt gttttcagct attccttctt
     9181 cttgctgaaa gtccttgagg ttctttctta caggatacca tcaaggtgtt caatggctta
     9241 ttcccattga atgaccgaga cttactatca gatttctgct cgttgaacca agtctttgat
     9301 acaggatttc ttccgtcatc ttctcacaaa taccaccttg cgtttctcta gcgattggtc
     9361 gatgtgcacc ctcccagtgg actgtcacta cccctttttg ctatgcctag cccacaaaaa
     9421 aagctgtcct cacagacag
//
LOCUS       DANJMI010000086         9322 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000086 DANJMI010000000
VERSION     DANJMI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.089"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..107
                     /locus_tag="PWU81_002779"
     CDS             <1..107
                     /locus_tag="PWU81_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HDL1111409.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="PWU81_002780"
     CDS             complement(176..904)
                     /locus_tag="PWU81_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1111410.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="PWU81_002781"
     CDS             complement(917..2197)
                     /locus_tag="PWU81_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL1111411.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="PWU81_002782"
     CDS             complement(2338..2940)
                     /locus_tag="PWU81_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL1111412.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="PWU81_002783"
     CDS             3185..3391
                     /locus_tag="PWU81_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111413.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="PWU81_002784"
     CDS             3703..4422
                     /locus_tag="PWU81_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL1111414.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="PWU81_002785"
     CDS             complement(4503..5186)
                     /locus_tag="PWU81_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDL1111415.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5405..7591
                     /locus_tag="PWU81_002786"
     CDS             5405..7591
                     /locus_tag="PWU81_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL1111416.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            7937..8347
                     /gene="spx"
                     /locus_tag="PWU81_002787"
     CDS             7937..8347
                     /gene="spx"
                     /locus_tag="PWU81_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL1111417.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            8361..8717
                     /locus_tag="PWU81_002788"
     CDS             8361..8717
                     /locus_tag="PWU81_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HDL1111418.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         3364 a         1525 c         1745 g         2688 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5341 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5401 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5461 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5521 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5581 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5641 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5701 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5761 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5821 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5881 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5941 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     6001 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     6061 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6121 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
     6181 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6241 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6301 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6361 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6421 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6481 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6541 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6601 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6661 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6721 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6781 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6841 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6901 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6961 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     7021 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     7081 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7141 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7201 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7261 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7321 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7381 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
     7441 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7501 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7561 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7621 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaatttcag
     7681 cttattttcg aagaagctat ttctggaaca cagtttattc ggaaaaggga ggtgtgacaa
     7741 gaattttgtc acacctccct tatttatttt gaaaatgaga atactattat caaaatagag
     7801 aatatataga aaaattctca ttattcaaac gtaaataatc agttattttg atttttttta
     7861 tattttatta gctaataatt ggtattttaa aagaaattac tgtagaattg gattataatt
     7921 aaaaaggagt gagaaaatga taaaagtata tacttcgcct agctgtactt cctgtagaaa
     7981 agcaagagca tggttgcaag cgaatcaact tgaatttgaa gagaagaata tttttgccga
     8041 accgttaaca gaaagtgaga tcaaaaagat cttggcagcg actgaaggag gagttcaaga
     8101 tattatttcc actcggtcga aaatttacga aaagctgggc atagatttta acgaattaac
     8161 tttaaagcaa atgatcgtct tattcaaaga atatccttca ttattgagac gacctatttt
     8221 gattgatgaa aaaagaatac taatcggatt taatgaagat gagatccggt catttgtccc
     8281 tcgtgaaatt cgatgtgttg ccagaaatct gatctattta caaaacaata acatcttaaa
     8341 ttcataggga gggagccttc gtggaagtga aagtctatgg atcagcgcac tgtgtaacaa
     8401 gtctgaaggc gaaacaatgg ttagaacacc atcaattgcc ttatcaattt gttgacttag
     8461 aagaacgcga tttaagtaca gaagaagcag aagaattatg cagttttgaa gaagtgcaag
     8521 cagaacagct atttgcgact tggtctgagg cttttcacaa cattgcgatt gacctttcct
     8581 gtgtaggaaa aaaacaactg atttttttat gcacacaaca taccaatctc ctcagacgtc
     8641 ctttgattat tatcgatgaa gttctattta ttggatacaa tgagcagttg atggaacagc
     8701 ttatcttaaa taaatagact ttaactgttt taatcaagtc actgagcatt tcatacagtt
     8761 gtaacagtcg aacacttagc atcaagcgtg ttatgtgttc tttttttgac tttatttttc
     8821 tactatagag acatgctcaa tttatgtggt caaaattcaa atctaggtgg ggatatagag
     8881 ttaaaaatat attgacatgt gtaaatttac atgatatatt catttctgtt gttttaagta
     8941 gcaaagcaat aaaatagtat tttaaaaaac atataaaata gttgttgaca tgttagaaat
     9001 gacatgataa gataactgag ttgttaaaaa agaaccactt gtgaagcttg attctatcga
     9061 aaaaaacttc aaaaaagttc ttgacaaata acaaatgata agttatgata aatgagttgc
     9121 tgtaagattt cgaaaaacga aataaaaaaa acttttaaaa aagagttgac aattacttgt
     9181 ctctctgata tgatataaaa gttgctacgg cagccaaaaa cgaatcgggg aacactcctg
     9241 atgaagtaga cctttgaaaa ctgaacaaag taagacaaac caaatgtgta gggcgtcttg
     9301 attcaattca agacaacaaa ca
//
LOCUS       DANJMI010000087         8942 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000087 DANJMI010000000
VERSION     DANJMI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.090"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(15..1154)
                     /locus_tag="PWU81_002789"
     CDS             complement(15..1154)
                     /locus_tag="PWU81_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDL1111419.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1553..2851)
                     /gene="eno"
                     /locus_tag="PWU81_002790"
     CDS             complement(1553..2851)
                     /gene="eno"
                     /locus_tag="PWU81_002790"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL1111420.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2967..3722)
                     /locus_tag="PWU81_002791"
     CDS             complement(2967..3722)
                     /locus_tag="PWU81_002791"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL1111421.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3826..5016)
                     /locus_tag="PWU81_002792"
     CDS             complement(3826..5016)
                     /locus_tag="PWU81_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL1111422.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5126..6127)
                     /gene="gap"
                     /locus_tag="PWU81_002793"
     CDS             complement(5126..6127)
                     /gene="gap"
                     /locus_tag="PWU81_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL1111423.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6168..7259)
                     /locus_tag="PWU81_002794"
     CDS             complement(6168..7259)
                     /locus_tag="PWU81_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HDL1111424.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7408..8742)
                     /gene="rpoN"
                     /locus_tag="PWU81_002795"
     CDS             complement(7408..8742)
                     /gene="rpoN"
                     /locus_tag="PWU81_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDL1111425.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8802..8873
                     /locus_tag="PWU81_002796"
     tRNA            8802..8873
                     /locus_tag="PWU81_002796"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8835..8837,aa:Arg,seq:ccg)
BASE COUNT         2317 a         1897 c         1647 g         3081 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgcccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tattttattt atacgagagg acttgcccat agattcctat
     1321 tctactgttc tatccttaaa agctcttata tgatgctagt tattgttagc tttccttttt
     1381 caaggatgat cttttcttgt accattcttt gtaaacatca tatacagaag ctagttgcaa
     1441 aaaaactctc tacctgctga tatttcagca agtagagagt tcgattactt aactaagtga
     1501 tcatactcga ctatctttca atagtcgatc atgtatcttc attagaaaag aattatttgt
     1561 tttttaagtt gtagaaagat ttcaaacctt tgtattctgc aacttcacca agttggtctt
     1621 cgatacgtag taattggttg tatttagcaa tacggtcagt acgtgataga gaaccagttt
     1681 tgatttggcc agcgtttgtt gcaacagcga tatcagagat tgttgaatct tctgtttcac
     1741 cagaacggtg agatacaact gctgtgtagc cagcttcttt agccatttcg atagcttcga
     1801 aagtttctgt taatgtaccg atttggttca ctttgataag gattgagtta gcaattcctt
     1861 tttcgatacc ttcagataat ttttgagtgt ttgttacgaa caagtcgtca ccaactaatt
     1921 gaactttgtc gcctaatacg tcagttaatt tcttgaatcc gtcccagtcg ttttcgtcta
     1981 atccatcttc gattgagatg attggatatt tagaaactaa ttcttcgtag aatttgatca
     2041 tttcgtcagt tgttttttcg ccttcgcctg agtcagctag tacgtaaaca cctttttctt
     2101 tgtcgtagaa ttctgaagaa gcagcatcca tagcaagaac aacgtcttta ccaggtacat
     2161 agccagcttt ttcgattgct tcgatgatta cttcaaaacc ttcttcgttt gaaccaaggt
     2221 ttggagcaaa tccaccttcg tcaccaacag aagttgctaa tccgcgagct ttcaagattg
     2281 aagctaatgc gtggaatact tcagcaccca tacgtagagc ttctttgaat gttggagcgc
     2341 caacaggcat gatcatgaat tcttggaagt cgattgagtt atcagcatgt gatccgccgt
     2401 tgatgatgtt catcattggt gttggcaata cttttgtgtt gaatccgcct aggtagtggt
     2461 ataaaggtac ttctaggtag tcagcagctg cacgtgcaac agcgatagaa acacctaaaa
     2521 tagcgttcgc acctaattta cctttgttag gagttccgtc taaagcgatc atagctttgt
     2581 cgatagccat ttggtcacga acatcgtagc caatgattgc ttcagcaatg atgttgttta
     2641 cgttgtcgac agctttagtt acacctttac cgccataacg agctttgtct ccgtcacgta
     2701 attcaactgc ttcgtattca ccagttgaag cacctgaagg aaccattccg cggccaaaag
     2761 ctccgctttc tgtgtatact tctacttcga ttgttgggtt accacgtgag tctaagatct
     2821 cgcgagcgta aacatcagta ataattgaca tgttttgtct ctcctttgag tttgtttttt
     2881 cctaagggat ttccctcata tacgatttta gttaattttg ctcattagtg caaacatttc
     2941 ttgcattttt cgcaaatttt caacaattat tttacagcgt ctaacaatga caagaatgaa
     3001 tctgcttgca agcttgcgcc accgactaaa gcaccgtcaa cattttcttt tgccatgtat
     3061 tcagcaatat tttctggttt tacagaaccg ccgtattgga tacgaactgc ttctgatact
     3121 tcttttccat aaagtttttc aactgtttga cgtacaactc cacaaatttc gtcagcgatt
     3181 tgtgcgtcag ctgatttacc agtaccgatt gcccagattg gttcataagc aataaccatg
     3241 tttgacacct gttcagctga aagatcagct aaaccagctg taatttgtcc ttcgatccat
     3301 tcagcagttt tgcctgcttc ataggtttct aaagattcgc cgcagcatag gattggtgtc
     3361 ataccatttg caaagatagc tttcgctttt ttattgatgt cttcgtctgt ttcatggaaa
     3421 tattcacgac gttctgaatg tccgatgatc acataatgaa cacctagatc agcaagagct
     3481 gctggtgaag tttcaccagt gaacgcacct gagttttccc agtagcagtt ttgtgctgag
     3541 atttttagtt ctgtaccttc agctgatgtc attaattctt gtaagaataa tgcaggagat
     3601 ccgatgactg aatcaacttt gtcatttgaa gggattttgt tttttactgc ttcagcaaat
     3661 tcttttgctt ctgatgcagt tttgttcatt ttccagttac ctgcgataat aggtttacgc
     3721 attgaggaac acactcctta atttattgaa aaatggctag ggagtcatga aaactccctt
     3781 caccttttgt tcgattactc gtcagaattg agcaaacttg gtcgtttatt tatcgttgat
     3841 tgctgctagt cctggtaatt ctttaccttc aagcaattcc aagcttgcgc cgccacctgt
     3901 tgagatgtgt gtgaatttgt cagcaaatcc taattgttct gctgctgcgg ctgagtctcc
     3961 gccaccgatg attgttgttg cgccttctaa gttagcgatt gcttcacaaa caccgattgt
     4021 acctttagca aagttactca tttcaaatac acccataggt ccgttccaaa caactgtttt
     4081 tgcaccttgt aatgtatcag caaataattt tactgatgct gggccgatgt ctaaagccat
     4141 caaaccatca ggaatgtctc cttcgattac ttgtgtttca acatcatttg aaaattctgg
     4201 tgcacagaca ttgtcgattg gcaagacaag tttgtcgcct gctttttcga ttaattcttt
     4261 tgctagttcc actttatcag cttctactag agagttaccg atcttgattc cttttgcttc
     4321 gtagaatgtg taagtcattc cgccaccgat caagatttta tctgctttag gaatcaagtt
     4381 ttcgattacg ccgattttat cagaaacttt tgcgccacca aggattgcaa tcattggtcg
     4441 ttttggttct tctactgctt caccgatgaa cttgatttct ttttccatca agaatccagc
     4501 aactgttggg ataccagttg aagcgatacc tacgttagaa gcatgtgcac ggtgagctgt
     4561 accgaaagca tcgttaacaa atacatcgcc taatgaagcc cagtatttac ctagttctgt
     4621 atcatttttg ctttcttttt taccgtcaat atcttcaaat cgtgtatttt cgaatacgac
     4681 aacgtcgccg tcattcatgt tgcggattgc ttcttctaat tgttctccgc gagtttcagg
     4741 tacgaatgtt acttctttac ctaataattc gcctagacgt tttgcaactg gagctaaaga
     4801 ttttccttct ttatcttctt ctgtttttac acgtccaagg tgagagaaaa ggattgcttt
     4861 tccgccttgt tctagaacgt agttgattgt tggcaatgct gctttgatac gagtgtcgtc
     4921 agtgatcacg ccgtctttca aaggaacgtt gaagtcaaca cggacaagaa cttttttgtc
     4981 ttttaaatcg atatctttca ctgttttctt agccatttta tttcctcctg aataaactta
     5041 ttatcaaaaa aagcggaaga agcgcgattg cttccccgct tttaaatgta catgtctata
     5101 atttacactt gtacgtttgt ctatcttata agttagcgaa gtattctaaa gtacgtacta
     5161 attgtgcagt gtatgacatt tcgttgtcgt accaagcaac agttttaact aattgtttgt
     5221 cgccaactgt cattacttta gtttgtgtag catcaaataa tgaaccgtaa gtcataccaa
     5281 cgatatctga agaaacgatt tcgtctgtgt tgtatccgta agattcgttt gaagcttctt
     5341 tcattgctgc attgatttcg tcaacagtta cttctttttc aagaactgtt actaattctg
     5401 ttaatgaacc tgttggtaca ggaacacgtt gagcagctcc gtctaattta ccgttcaatt
     5461 cgggaattac taaaccgata gctttagcag cacctgttga gttaggaacg atgtttgcag
     5521 cagcagcgcg tgcacggcgg aagtcacctt taggatgagg tccgtctaga gtcatttggt
     5581 cacctgtgta agcgtggata gttgtcatta atccttcaac aacaccaaat ttgtcgttca
     5641 atgttttagc cataggagct aaacagttag tagtacatga agcacctgag ataactgttt
     5701 ctttaccagt taatgtttcg tggtttgtgt tataaacgat tgttggaaca tcgtttccgc
     5761 caggagcaga gataacaacg cgtttagcac cagctgttaa atgtttttca gctgctgatt
     5821 tagaagtgaa gaaaccagta cattctagaa cgatatctac gcctagttcg ccccatggta
     5881 attcttctgg gttgcggtta gctaatactt taacttcttt tccgtttacg ttgaatgaac
     5941 cttcatgaac ttcaactgtt ccgttgaaac gtccttgagt tgtgtcgtat tttaacaagt
     6001 gtgccaacat tttagcatct gtcaagtcgt tgattgctac tacttcgatt ccatctacat
     6061 cttggatacg acggaatgct agacgtccaa tacgtccaaa accattaata cctactttaa
     6121 ctgtcattta agatttcctc cttatgaaaa tcaaaaaaat atttatttta aagggttacc
     6181 ccttttaaaa tctcatttgc agcagcttca tcagtgatga gccacgtttg ttttggtgca
     6241 tttttcatat aagctcgaat tgctttagct tttgattttc cgcccgcgat tgccatgatg
     6301 atcgggactt tttccaaatc ttgcaattct atgccgattc tagggacctt ataaacgact
     6361 tcaccttttt cattgaaaaa gtagccaaaa gattccgcaa cagcatttgc ttgtttcagc
     6421 atgactaatt ctttttcagt catcttacga cgtgctgcca tgtgtaatgc acgcccaata
     6481 ctatgaataa cacaattcgc ttcactaatc aagtttaaga cttcttggat agatggctca
     6541 ttgagtaaag aattgtaagt tgccagactt aattgttccg gaacataaag tgcgcggtgg
     6601 ttaccgtttg ctttagcagc cattcttgca ctgacagaat ttgcctgtac agttatcgct
     6661 tcaccaatcc cgcctcttgc agggacaaac aggttatgcc gtttcttatt ttccagatta
     6721 gacaattgtt cggctaccac agccatagta gttcctccca tcacagcgat gatattctcg
     6781 ccttctggaa gtagtcggtc taaggcttcg ctcagcgctt cgccgaatgc atcagccact
     6841 ttggtctgct cttcgctgtt tcctgaaaca acgatacagc gttggttacc aaaatattct
     6901 gccaactgtt gttcggtttg atgcgtacct aaaagctgat ccatgaagtt ttcaaggctt
     6961 tgataaactt cttcgccctt agttgtcagc gtcataccgc tttttgaggt gtcgatcaaa
     7021 ttcagatttt tcaaaaggtc tgtttctgtt cgtaagactc gttctgtgat ccccatactt
     7081 tccgataaac ttcgacgccc aatcggctgc atccaataga tatttcgtaa aattttatag
     7141 cgttcttgca acacttcgat aatatctggt gcaacagatt caatcatttt tatttcatcc
     7201 agcatagcga tctccttagt tggacacttt acgtccaatg tagacttaaa acgacccata
     7261 tacgataaaa aaagaaatta actattcgaa tttcacatca ataaagggaa atctcaacct
     7321 cacgactgta agtctaacag ttttgctctc gtttttcaac taaaaagact agaaaaagtg
     7381 aaatactttt tctagtcttt tgactgatta gttatcataa cgttttcttt ttgatgaact
     7441 cgggattcct aataaatcac gatattttgc tactgttcgt cttgagatcg ctatctcatc
     7501 ttttttcagc aattcaacca atttctggtc agacaacggc ttgtttttgt cttcttgatc
     7561 gactaattcc tgcagctttt tcttcgctgt atctgctgaa aggtcttctg tctgttctgt
     7621 actattcgta ggaattcgtg tagtgaaaaa tttttttaat tcaaatacac cgaagtctgt
     7681 ttccaaatat ttcccgttga ctgcacgact gaccgtggat tcatgcacat ctatctcagt
     7741 tgcaacttcc tttaacgtaa gaggcttgat tggacgttcc ttgtcgataa agaaagcttt
     7801 ttgccgggaa acaatgactt gagcaacccg caaaattgta tccccacgtt gtaggatcgt
     7861 tttcttcaac cattcgaatt cctgtttccg ttcttttaaa taattttcag tttctttatc
     7921 tgcttgttgt gacatttgtt tgaagtaact ctcttgaaag cgtatatttg gctgattttt
     7981 tcggtttgag ataacttgta catttttatc gtcgtccact aatacccgga catcaggaat
     8041 aatgtacaat tcgctgctac ggtcgaaaat ccggccaggg cttggactca acgtttgtaa
     8101 atagtcgaat atctgctgaa cagcatgtag atcaacagaa aaacgttgtg cgatttcttt
     8161 ccattttcgc tccaccagtt cctcaaaaca ttcttccaac acgatataag ctaattccgg
     8221 cgcataatca tctcgttctg tttgaagcat gagacattct tgcaaactac gtgcacctac
     8281 tccagctggt tctaattgct gaatcagagt caacgcatcg agcatttgga taggcgtagc
     8341 tccaatctct tttccggctt cttctaaact aatcgtcaaa aatccgttca aatcgatgta
     8401 ttctacaaga taaagaacga ttttcctcaa aaaagtatct cggtaattca aatggacttg
     8461 ttcgatcaat gaatcaaata aagacccttt tgtatcagga atctgattta ggtaatttgt
     8521 ctcttctcct tcataagaag atgaagaagc ttttatgtaa tctggctgcc attccggctc
     8581 aacaacttcc accaatggat tttccattgc ttgattctca acgaatgcca acagctcctc
     8641 actgttatat tgtaaaactt ggatggactg ttgcaactgt tgcgtcatgg ctagtttctg
     8701 tgtctgctgc tgtttttgtg aaaaattttg ctcaaatttc atttttttgt ccaatcccct
     8761 tgtaattttt tatctgttta gttacaatta tcccagtgct tgcgtcctta gtgtagtgga
     8821 tatcacgtaa gattccggtt cttgagacgg gggttcgatt ccctcaggac gcgttctact
     8881 gctattttag cataatttac gaatagttgc ttctttattt ttaagtgaaa actgtccgcc
     8941 aa
//
LOCUS       DANJMI010000088         8733 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000088 DANJMI010000000
VERSION     DANJMI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.091"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(39..635)
                     /locus_tag="PWU81_002797"
     CDS             complement(39..635)
                     /locus_tag="PWU81_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL1111426.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            985..1329
                     /locus_tag="PWU81_002798"
     CDS             985..1329
                     /locus_tag="PWU81_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL1111427.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1330..1749
                     /locus_tag="PWU81_002799"
     CDS             1330..1749
                     /locus_tag="PWU81_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL1111428.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1841..2191)
                     /locus_tag="PWU81_002800"
     CDS             complement(1841..2191)
                     /locus_tag="PWU81_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111429.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2157..3488)
                     /locus_tag="PWU81_002801"
     CDS             complement(2157..3488)
                     /locus_tag="PWU81_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HDL1111430.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3743..4309
                     /locus_tag="PWU81_002802"
     CDS             3743..4309
                     /locus_tag="PWU81_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1111431.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4454..4541
                     /locus_tag="PWU81_002803"
     tRNA            4454..4541
                     /locus_tag="PWU81_002803"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4486..4488,aa:Ser,seq:cga)
     gene            4877..5851
                     /gene="bsh"
                     /locus_tag="PWU81_002804"
     CDS             4877..5851
                     /gene="bsh"
                     /locus_tag="PWU81_002804"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDL1111432.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            6176..8071
                     /gene="asnB"
                     /locus_tag="PWU81_002805"
     CDS             6176..8071
                     /gene="asnB"
                     /locus_tag="PWU81_002805"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDL1111433.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8266..8712)
                     /locus_tag="PWU81_002806"
     CDS             complement(8266..8712)
                     /locus_tag="PWU81_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL1111434.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
BASE COUNT         3071 a         1483 c         1628 g         2551 t
ORIGIN      
        1 aacattgtca atcaatgact aattttacaa aataaatatt atccaagaag accacttaga
       61 tattgacctc cataaatcaa ggccatacta tataagaaaa tcgacagtgg tttagcaatc
      121 aaaatgatta gtgtatatcg tttcactgac atgtttgtca atccagccat caaacataac
      181 gcatcgtcag gagcaactgg aagaaaaata gctagagcaa acagcttctc aaaacggttt
      241 tgattatcca gccagccaat atatttgtta tatgtcttat cgctaatcat ggaaagtatg
      301 aatggctttc cataacgacg ccctagcaga aaaatgataa tcgaaccaat gcagattcca
      361 atatagttat agataaatcc agcataaggg ccaaatatca ggacaccagc tgcagtactg
      421 atccctccag gaatgattgg aataactact tgcaggattt ggatgaagac aaaaataatc
      481 ggtcctaaaa tgatggaatc acctactaat cctcttaacg cattcaaatc tttgaaaaca
      541 cctaaattaa taaaataaat cgtcagcgcg attgataggc ctaatccgat aatggatatg
      601 aaatttatca gttttcgaga ggcagcaata ttcattattc atcaactcct tagaggttct
      661 ttgttaacta tactttacta gaaaagcaac taactgcgcc attttttgat atgacagaaa
      721 acaaacaatt ttgtaagttc atcataagta taaaagattg atctcaaaaa aacatccaac
      781 taaagactta tttaataact tgtttttttt aaaataaaaa aagcgttagc aactatgctt
      841 aaaaagcata tcgactgaat aaactaagca ttggacaggc tgaaagacta catttaaaat
      901 gaaatgcgaa gaaacaatag aaaataaagt tgccttagaa ccatagttga atcacctaac
      961 agtcgtgaag ggagaataag agaaatggca gaaaaacaaa ccgcaggacg agatcaatta
     1021 ggagattttg caccaaaatt tgcagaatta aatgatgatg ttttatttgg acaagtatgg
     1081 tccagagaag aattatctgc tcatcagcgt agtatcatca ccatatctgc attaatttca
     1141 ggaggaaatt ttgaacaatt gccagctcat ctgaagattg gcaagcaaaa tggcattact
     1201 agtgaagaaa tagcagaaat cattacccat ttagcttttt atgtagaatg gccaaaagct
     1261 tggtcagctt ttagtttagc aaaagaaatc tatttagaag aaaatagcga agataagaaa
     1321 gaggcgtaaa tgatgacaaa acacgaagaa atacacaatg gcgtgatttt tccagcaggt
     1381 gaaaaaaacg aagcttacgc tcaagtattt accgggcaaa gctatctgaa tatgttagtg
     1441 aatgatcccg aagtttctgt tggtgtaggt aatgtaactt ttgaaccagg ttgtcgcaat
     1501 cactggcatg tccatacaga cggctatcaa atcttacttg tgaccggagg agaaggctgg
     1561 tatcaggaag aaggaaagcc agcaagacga ttaatggctg gagatgtcgt cgtaacgcac
     1621 aaagggatta aacattggca tggtgcgaca aaagacagtt ggtttgaaca tttggcgatc
     1681 actgctggta attcagagtt tttagaacct gtaacagatg aagtgtatga aggattggaa
     1741 aatcaataat gtcaagcttg aacagctcct gaaagtagat tttaatcgaa ctttcaggag
     1801 ctgctttttt gattcagtct tttataaata gaaaataggg ttaataatag gaatgagaag
     1861 cacgtcgaaa gatcttaata ttgataatcg gctgggcata catgatttcc acgtcatcaa
     1921 tcaaactacc taacagatga taatcttccc gatcatgtcg gggattacct aatagttctt
     1981 gatagtagat ttccatacta gggtcccgag gattgttcag caaatcagca agtacatcta
     2041 aatcttctgg ctgagtttct gccgtaccct gtatctctat gaaaagccct tcctcagcaa
     2101 attctactcc aatacgtatt tcagttattt catgggaata taaataagta agaagctcat
     2161 cgatgattct ttttgataat tctttttcca agtgtctttt tttcctttct gacagcggta
     2221 aagttatcta gtatcggtac aaggatacct gcaacgaaac cagttgaaaa accactgtta
     2281 tatagattta gtcctccgtg caaagtgctg acgttatgga caagagcaat atgtagcaca
     2341 ccagctatta caccgtaaaa actgccgtaa aaaccgctta tgggcgcaag acctgtccca
     2401 aacacagctg caacaagcat gccagtactg gtgatctcat gaaaagttcc aaacaaagaa
     2461 gcaataaata ttccgactag tatcggaaaa ctgtttttta aatgacaacc aaaagcactg
     2521 aaacccaccg cagataaaat agcaccgatg acaggtccat ttaactgacc gccaaccagc
     2581 aaaacataag aagacagcat cagaccaact aagcccatgt tgatcaaagt agcaccaata
     2641 ccagaattag caatgaaatc agttgtcaat tttccagatg aatcgaaaat ttctcgaatc
     2701 cccgaaagcc gaaaggaatt ataatagaaa ccaattccga ctgtcagcga aaaaaagcca
     2761 aaaataatcc aaatgagagg aaagtgatat tcattgaaaa ccaatgtatt ttcgacgatt
     2821 gaatagctga ataacctcaa aacagcagta aaagtcatcg caatcaaccc agaggtgaat
     2881 ccaatattat aaagtgtgaa tccacgatgg aaaactaaag tttgagcagc taagggcgga
     2941 agaagtatac caattgctat gccagctaaa ttccccaata aaatacctat cagtaaagga
     3001 aaacgcatac caaaagtgat ataactaacg accggtgata atacgctacc aaacagtccg
     3061 atcatgatgt actgagaaaa aggtttattg acaaatacag cgtatagata cacacctaaa
     3121 atgattgaga tagaattata aaggtttttg ccaaaaaaag agaaaccggt aactgttaga
     3181 atcgaagcaa ataagggacc gtttaaaatc accttgtgcc gataagctaa aagcacagaa
     3241 agtaaggtca aaatccctac atttatgaat gcgcttccga aaacacctaa agcaaaataa
     3301 tctgtcaaaa gattgcttgg cgaggtaaga atagttagca atccactcca attatcagta
     3361 ggagtattag aaagcaagcc aataaaaaga aaaaaaacac ctgtaaaacc aagataaaaa
     3421 tactttcttt tctgtgaaac gattcgtttt tgaggatgaa tataaataac gtcagtttcg
     3481 ttttccatta cacaccttct ttttcatttt ctttaactat aacttgaaat gaaagatttg
     3541 gacaactatc atttaggtca aaactaaaaa tgtaagggaa cccattgaat ttaaccaaat
     3601 ttttttaatt ttgcaaaaaa aagttaaaga agactgaaaa aaatttaaaa tataacgttt
     3661 tgagtaaaat gtttgcaaaa aatgctaaaa atagggtaca ataaaacaac atatactgca
     3721 gtaaagaagt gggggagttt taatgagaag aaaagtctat accaaggatc aaattctaaa
     3781 agctgcttat gatgttgttg caaaggaagg gtttaaagga tttactgcga gaaatattgc
     3841 taaaaagatg gggatttcga cacagccgat ttatctggaa ttcaaaaaca tggaagattt
     3901 aaaaaatacg ttgctggatt ctatctttga acaactaaaa gaagatgttt tccctattga
     3961 acgtactggt gataaaatca ttgatctcgg tttgaactat atctactttg caaaaaagca
     4021 tcatagtctt tacatggcat tgtacattga tgaacatggg gacggaaaga aaatgcatga
     4081 tttttcatac aattatttcc gtgaaatagt taaagaagat gaaagataca atcaattatc
     4141 ggatgaatat gtcgatgctc tgcatactgg tacatgggta gcagtgactg gcttagcgtc
     4201 attgatgtca tcaaacatca tgcatccaac agaagaacaa atcattgcac tgatcaaacg
     4261 gacaattgac tcgattttag cattagataa tcctgaaata atcagatagt gttctatcag
     4321 tatgaatttg atcggtaagt ccgttggctg caaactgtca atggactttt ttgctaaaaa
     4381 aagcgatttc tgttatttat ctattagaaa taggcttgac atttcgtgaa aaaagtttaa
     4441 aaatagtact tacggaaggt tggcagagtg gtaatgcacc ggactcgaaa tccggcgaac
     4501 cggctaatac cggcgcgcag gttcaaatcc tgtaccttcc ttatatcaac gattagaggt
     4561 tgtgaagaag ctgtcctgca tcaagtaata agaacggcca aacaaaaata gcttttccta
     4621 ttttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttgttcgg
     4681 aataaggggc tgtgacaaga cttttgtcac agccccttat tgttgtactt ttaacctaca
     4741 atattttttg attccccctg agtactattt tttataaatg ctagtaaatc aatcttcttt
     4801 ggcctttttt ttgataaaaa aaaatactag atgtaagata aaagtgtaaa aacgatttgt
     4861 tttaggaggg aaaacgatgt gtaccgcaat cacttatgca acgaaagacc attattttgg
     4921 aaggaatttt gattatgaga tgtcttataa tgaagtagtc actattactc caagaaatta
     4981 tcgtttcgat tttagaaagg taaaaaattt agataaacat tatgcaatga taggaatagc
     5041 agctggggta tccaactatc cactctatta tgaggctaca aatgaaaaag ggttgagtat
     5101 ggcaggactg aattttcctg gcaatgctga ttataaagaa ttacaagaag gaaaagataa
     5161 cgttgcacca tttgagttta ttccatggat actagggcaa tgctcaacca tagacgaagc
     5221 taaagaatta ttggctacta tcaatctagt caatatcgat tttagtgaaa aactaccctt
     5281 gtcaccttta cactggttat tagctgataa agaaaaatcg atagtaattg aaagtatgaa
     5341 agatggactt catttatatg ataatcctgt cggtgtgttg acaaataatc cgccatttga
     5401 ttatcaatta tttaatctga acaattatcg ctcgctatca aatggaacac cagaaaatca
     5461 tttttcaaac cagatcagct tgaatgttta tagtcgtggg atgggaggat tgggcttacc
     5521 tggagatctc tcttctgttt ctcgatttgt aaaagccaca tttactaaaa tgaatgctgc
     5581 atcaggtgat tcagaatctg aaagtatcag tcaatttttt catatactag gctcagtcga
     5641 gcaacaaaaa ggagtatgcg atactggaga aggaaaatac gaatatacga tttattcttc
     5701 ttgctgtaac gttgataaag ggatttatta ttatcgtacg tatgaagaca gtcaaattac
     5761 tgctgtaaat ataaataaag aagacttgga tcgtcatgaa ttggtcagtt atccagtcat
     5821 aaaagagcaa cagataaact atgtcaatta attaatgaat agatccgttt ggtatcatat
     5881 tttctgatac agtcataatt agacacgatc tttaggtcgt gtcttttttt gctgtttttt
     5941 ttggaaatag catccactaa tattttttta acacgaagag aataaggtag cctcaaaaaa
     6001 gaaaacatgg taaattagat attgggactt tattttctgt aaaaacagaa actttcatct
     6061 tttgttttca cgtgacgaaa ttgtaagaga gaaaatattc gtccgctatt tattctatga
     6121 taagatgcat atgaatgaaa taaacagtaa ttttatatta ggatgtgaaa gttaaatgtg
     6181 cggtattgta ggttttgtta acgacaagga caacaaaaaa acaattatta atacaatgat
     6241 ggatcgaatc gttcaccgtg gacctaatag ttcaggagaa tatatcgata aacatgttgc
     6301 tttaggattc agaagattaa gtattattga cttagagggc ggtactcagc ctatttacaa
     6361 tgaagatcgg acaaagatca ttatttttaa tggcgaaatc tataattacc agcctttgag
     6421 agaagaattg attgcagctg gccacgtttt tcaaacacat gcagatacag aagtcctttt
     6481 gcatggttac gaagaatggg gaacagaatt attgcaaaag atccgcggaa tgtttgcttt
     6541 tgctatttgg gataatgaaa agaatgagtt attcggtgca agagaccact ttgggatcaa
     6601 accatattat tatgcagaaa tgaacggaac atttatgttt ggttcagaaa tcaaaagttt
     6661 tctgcctcat cctgatttta acaaagaatt gaacaaagaa gcgttgaaac cttatatgac
     6721 tttccaatat tctccattaa acggcgaaac attcttcaaa gatgtctatc gtttgcctga
     6781 aggccattat tatacgtata aagatggaaa actagacatt cagcaatatt gggatgcaga
     6841 ttttgaaaca aaagaaacgc attcccgaca agaatggatc gaaaaaatcg acgaaaccgt
     6901 tcaagcttct attgaagcac atacggtcag tgatgtagaa gttggttctt tcttatccag
     6961 cggggttgat tccagttatg taacctctgt attgaaacca gatcattctt tctcaatcgg
     7021 gtttgatgat aaaacctata atgaagcgat tgaagccaga aaactaaccg aactattgga
     7081 cttagataat acagcagcag tgatcgacgg agacatgtcg ttcaaagcat tccctttgat
     7141 ccagtatcac ttagatgaac ctgattcaaa tccttcttgc gtacctttgt actttttagc
     7201 aaatcttgct tcacaaagtg tgcgggtagt ccaatctgga gaaggggcgg atgaactgtt
     7261 tgcgggatac caaacgtatg gattccatac gaattcaaaa ttcatccgtg tgattgctca
     7321 aggattgaaa aaattaccaa aaggaacacg ctataattta ggacgtaaaa tcggaaaaat
     7381 gaaaaatttc catggacgga tccatttata tgaatcactt gctcctgcaa aagaatattt
     7441 tatcggtcat gctcgagtgt tcgaagaatc agaagcatca gaagtgttaa caccaaaata
     7501 tcaaacagca ccatctgtgg atgaaatcat gactgttcat tacgagaaaa cagaaggaat
     7561 caaagatgaa gtcaataaga tgcaatatgt cgatcttcat caatggatgc caaaagatat
     7621 cttgttaaag gcagataaac tttctatggc aagttctttg gaagtccgag tacctttatt
     7681 ggatatcgaa gtcatgaaat tggctcaaca aataccaagt aagtttttat tgaatcaaaa
     7741 caacacaaaa gatattttca gacaagctgc gaacaaacat cttccggaag aatggtcgaa
     7801 tcgtgtgaaa ttaggattcc ctgtgccaat caaagcttgg ttgaaagaag aacacggcta
     7861 cgaacaagtc aaagcattat tcgaagcaga ttttgcaaaa gaattttttg atcaagaaaa
     7921 aatcatgaag ttgctagatg accatcacga aggacgtaaa gaagagcaaa gaaaaatctg
     7981 gacaatcttc agtttcttga catggtataa agtctacttt gtagatgaaa cgattccgca
     8041 agcagaagca atcgactatg tgactgtttg atgaaaatca aatatcaata aaaaagaggt
     8101 tttctcctac cgtttaacac tgagtgataa gaaacaattt caaaaaacag ctcttcccgt
     8161 tttatagata ttatgactta tggttcggag ttggcagagg aacaccattg tttcgaacat
     8221 aagggggttg tgacacgatt ttcgtcacaa cccctttaca tatgttcaat aatcgatttc
     8281 tttttctttt gtcccatctt ttttataaat gaacaaagtt gattctttgt tttgtgctac
     8341 ttgctttcct ttttcgattg cttctttttt tgtatcaaaa tggttgcttg ccttttttgc
     8401 ccctttagat ttcacgatcc attgttcttc ctcatattct acgatcacat ctgaatcaag
     8461 cagttttcca gcacgaggat tcgtatcgtg ttcatccgtc ttgcttggat ttttttcttt
     8521 ttcgaaagtt cgtttttctg attcactagc atcttcatac catttttttg cttgatcaat
     8581 ggcaattggg atcgcacggt tatcagggta cccttcatct aataatgcat ttgcgatgtc
     8641 gattgctttt tttcttgtca atttatctaa gtttttcatt gatgcaggaa agtccttcat
     8701 gttccatggc atattagtat ccccctttat taa
//
LOCUS       DANJMI010000089         8418 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000089 DANJMI010000000
VERSION     DANJMI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.092"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            331..1254
                     /gene="truB"
                     /locus_tag="PWU81_002807"
     CDS             331..1254
                     /gene="truB"
                     /locus_tag="PWU81_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL1111435.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1258..2199
                     /gene="ribF"
                     /locus_tag="PWU81_002808"
     CDS             1258..2199
                     /gene="ribF"
                     /locus_tag="PWU81_002808"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDL1111436.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2594..3379
                     /locus_tag="PWU81_002809"
     CDS             2594..3379
                     /locus_tag="PWU81_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1111437.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3392..4243
                     /locus_tag="PWU81_002810"
     CDS             3392..4243
                     /locus_tag="PWU81_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDL1111438.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4367..4819)
                     /locus_tag="PWU81_002811"
     CDS             complement(4367..4819)
                     /locus_tag="PWU81_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1111439.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5284..5652)
                     /locus_tag="PWU81_002812"
     CDS             complement(5284..5652)
                     /locus_tag="PWU81_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HDL1111440.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5802..6284
                     /locus_tag="PWU81_002813"
     CDS             5802..6284
                     /locus_tag="PWU81_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HDL1111441.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6321..7331
                     /locus_tag="PWU81_002814"
     CDS             6321..7331
                     /locus_tag="PWU81_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDL1111442.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7446..8159)
                     /locus_tag="PWU81_002815"
     CDS             complement(7446..8159)
                     /locus_tag="PWU81_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111443.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
     gene            8309..>8418
                     /locus_tag="PWU81_002816"
     CDS             8309..>8418
                     /locus_tag="PWU81_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1111444.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT         2862 a         1366 c         1703 g         2487 t
ORIGIN      
        1 tgattaagaa actgaaaaaa taaataaaaa aaaacggaaa aaagactgga agaatggaca
       61 gtcttttttt tcgtttttgg gaagtgaaaa ccaaactatt tacctgtaag ccacccataa
      121 atccaatcaa atggtgcaaa gaaaaaatct tccattctat tccctccttt aaaaaaaaca
      181 taacatgatt tctatttacg tgagcatgaa ttttgttacg tttttattgc cgaaaaataa
      241 aaaccgttca ttatgatgtc aaattcgtca gtaattgatg caatgtgtta taataattca
      301 gttaaatcaa aaatgaaatg gagaaaagac atggacgggt tattaacttt atggaaagaa
      361 cgcggaatga caagtcatga ttgcgtattc aaattaagaa aaatcttaca tacgaaaaaa
      421 atcggacatg ggggtacgct tgacccagac gtagacggtg tgttgcccat ttgtattgga
      481 aaagccacaa aagttatcga atatttgaca gattctggga aaatctatgt aggcgagata
      541 actctaggtt tcagtacaac gacggaagat cgatccggag aacgggtaga aacaaaacca
      601 gtagatatgt ctttatctga agaaaaaata gatgaagtga tggcttcatt tattggtgag
      661 atcacccaga taccaccaat gtactcagcg gtaaaagtca acggtaagcg gttatatgaa
      721 tatgcccgca ataatgaaac agtggagcgt cctgttagaa aggctcagat ttaccgtttt
      781 atccggacaa gtgaattgac aagagacgaa gtgacaggaa ccatttcttg gcgcttcgaa
      841 gtagaatgcg ggaaaggaac ctatgtccgc acattagctg tagatacagg caagaaactt
      901 ggttaccctg cgcacatgtc tgatctgacc cgaacagcaa gtgccggtat gtcccagaaa
      961 gatgctctga cattagaaga agtagctcaa gctgtgaaaa atgggacaat cgatgattgt
     1021 ttgctgccaa tagaaaaagg agtgaaacga ttcaaacgag tagatattac tgatgaagta
     1081 tggcagaaag taaaaaacgg tatgagactt aattatactg tatttgcgct cccagaaatg
     1141 ccgtcagaag aaattgcttt gttttataaa ggcaaagttg tcagtatcta tcagccaaac
     1201 ccgaaagaaa aaaacaaatt aaaacctagt aaagtattga gaaatgagga ataacgaatg
     1261 aatatcatca agatcagaca tccttaccaa tcagaacaaa ttccagaaga cgatgtcgtt
     1321 ttagtactag gattttttga cggcgtgcat attggacacc agaaagtcat aaagacaggt
     1381 atggaaattg cacaaaaaga aggattgaaa ttagcattga tgactttcaa tcagcatcca
     1441 tcgattgtct ttaaaaagat cgatcctagc agcgtgaaat atttgactag ccttaagcaa
     1501 aaagaagaaa aaatggccgc tttgggtata gattatttat atgaagttga ttttacttct
     1561 tcttttgctc atttagcacc gcaggaattt gttgatcaat acatcgtagg gttacatgca
     1621 aaatatgctg tttccggttt tgattatacg tatggaccaa aagacattgc agatgtcgct
     1681 cattttcctg aatatgccaa aggaagattc aaaatcgtca cagtttctaa agaagaagaa
     1741 caaggtgaaa aaatcagctc tacccgtatt cgaggattac tagacagcgg acaagtaaaa
     1801 gaagcagcaa aactattagg ttgcacatat gaggtagaag gggtagttgt ccatggcgat
     1861 gcacgaggca gactactagg attccctaca gcaaatgtca aagtaaagag cacagttcat
     1921 ttgcctaaag aaggtgttta tgtatcggaa ataaaaattg gggaaatttg gtatccagcc
     1981 atgggttcaa ttggacataa tgatacattc ggtgaaggac gggagctgac tgtagaattg
     2041 tatattttgg actttaatca agatatctat ggtgaacatg tcgcagtacg ctggaatcaa
     2101 tttttaagag atcaagtgaa attttctagt gtagaggaat tgattgaaca actcaaagag
     2161 gatgaacaag caacagctga atatttttct cagcaataaa acattatgca taaaatctct
     2221 tgtttatgta taactagcaa tttcaataaa atgggtgata aataagccca ggaaaggggc
     2281 tgtgacaaaa gtcttgtcac agcctttttt tccaaataaa tggtttttca gaagtagctt
     2341 cttcggaaat aagccgaaat ttcacaaaaa tttgagaagt aatttttgta aattacttct
     2401 tatttcttga agctggccac ttctgtcaca acctctttct ttatagaaat aaaaggacac
     2461 tagcaattca tgaaaacgat tgctaaagcg tatcatttgc tttagactat atatcttggt
     2521 gtacactcac tttaagacgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa
     2581 aggagtgggt actatgtata aggaattaga aaacaaagtc gcagtagtaa ctggtggctc
     2641 aaaggggatt ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt
     2701 caactataac tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg
     2761 cggaaaagca gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact
     2821 tgatacggct ttagaaaatt ttgggacact ggacgtttgg attaataatg ctgggatgga
     2881 aaatcagcat ccaacccatg aacttagcct tgaagattgg gaaagagtat taaaagtcaa
     2941 tttgactggc gtttttatcg gaacgaaagc ggctttaaat tactttacag agaatgataa
     3001 aaaaggaaat atcatcaata tgtcttctgt tcatgaacaa atcccatggc caacgtttgc
     3061 tcattacgct gcttcaaaag gcggagtaaa actattcacc gaaacggtag caatggagta
     3121 cgcaccaaag ggaatcagaa tcaatagtat aggacctggt gctatccgta cacctattaa
     3181 tgcggcaaaa tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg
     3241 acgcgtaggt gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc
     3301 cagttatgtt acaggtatta cactatttgc agatggtggg atgactttat atccttcttt
     3361 ccaaggtgga aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc
     3421 agttgggtgg gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga
     3481 gatattagga actggtattg gagccgtgct cgttggctta cttgttcaat tattcgcttc
     3541 tccagctggt atttcagtga ctacattttt gattagttta ctttctggtg cattctgggt
     3601 catcggacaa acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc
     3661 aatttcgact gcattacagt tagtcggaac ctcattgatt ggtgtcttcg cttttggaga
     3721 atggtctgga agtatgggaa aaatcattgg tgcagcagca atcattcttc ttgtatttgg
     3781 ttctgcttta acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat
     3841 cagtctatta gcatcaacaa gtattggtta ttgggtttac agtgcattgc ccaaactagt
     3901 tgatgcaagc ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt
     3961 gatttatgtg attttccgaa aaccaaccgc attttctgaa gacaagagtt ggaaagcaac
     4021 aatagttgga gccatcttca gtgtgtcagc attggcttat attttttcag caaatgaaaa
     4081 tggggttgct actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg
     4141 tatgtacatc ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt
     4201 attgctgatc gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa
     4261 tctcatttat taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc
     4321 gctagaattt ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaagtg
     4381 aaatatctgt ctgcatcaat aatttttcat tgatccattg actaaaattt acgacatgat
     4441 tgaagttacc accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa
     4501 gacggtcttt tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggattt
     4561 gattgattgc tgtaaaatca cgatactgaa ggttgaaacg catcgtgatt ggtgcattta
     4621 gcatgaatcg ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa
     4681 aaagattacg ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt
     4741 catgaagata atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta
     4801 ctgagtaaaa atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga
     4861 tatttgctta atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca
     4921 agtcagaaaa ttctaacttt tctttctcta aagaaattcg ttctctccta aagtgatatt
     4981 atatttaaat atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg
     5041 tattttttcc ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt
     5101 ggaaaattca aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa
     5161 aatagcagat actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga
     5221 acaaaacctg tataagctga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa
     5281 gctttatcgc caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg
     5341 ttcaatcaat tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata
     5401 tacgtagcct gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc
     5461 gatagaaaaa tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa
     5521 cataggttgg ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc
     5581 aaaaaggtct ttgatctttt gttgatgttc tttttctttg atctgattga tgatcggctg
     5641 aaatttttcc attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca
     5701 atagttatgt ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg
     5761 ataagatgtc tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga
     5821 aattcttaca cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg
     5881 aagatgcttt ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat
     5941 tcttaaaaga taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg
     6001 atttcaaagc attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca
     6061 tggaagaaca aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga
     6121 ttccgattat tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta
     6181 gaccagatca aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg
     6241 agatgccgat atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc
     6301 atacttggag gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg
     6361 gggttactgc accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt
     6421 taaagagaat ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag
     6481 ataaggaaaa atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag
     6541 atgaaaatat ccaaatgatc tttccgcctt ggggaggaga attattgatt gagattctcg
     6601 attttgtcga ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta
     6661 cgttgctact tgctgtcaca ttaaaaacag ggatagccac tgcacatgga accaacctta
     6721 ttgatttaag aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa
     6781 caagagaagg agaatcgatt ctacagcaac cttctgaaaa gtatcaaaaa gagtggcccc
     6841 atgagcattc atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca
     6901 gcggagatgc agtaaggatg aagggacgat tattaggtgg ctgtattgat acgatcagtc
     6961 atttaatcgg taccccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa
     7021 agccgatctt gtggtttcta gaaaattgtg agctatcgtc agttgattta agaagatcac
     7081 ttgttcagat gaaactggca ggatggtttg atggcagctc ggggatcatg ttcggtagaa
     7141 gtgcagcaga ggatttgtta gattataaaa tgaaggatgt atatgaagat ctgtcaaagg
     7201 aattaaaact accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat
     7261 tgatcaatgg tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa
     7321 cgtttattta aaaaattttt tcctgccttg gagtttatca aagaccaaca aatctaaata
     7381 gaaaagaaaa tgaaagaagc tgacagtatt ttgccagctt ctttttgtat gatctattag
     7441 aaattttaat tatccataac tatcacattg atcagctgtt tcacatcatt tggattacct
     7501 gtaggaatag ctactacaga tgcattatca ataatttctt gagttgtttc tctaatgaac
     7561 gctggataag atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg
     7621 attgtcccgt catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg
     7681 agcataacca catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt
     7741 cctgcaggat aatgaataac atgtagttcg aaatcacccg atgtatctct aaaagtatta
     7801 cctctaattg ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac
     7861 ggatctattg actgagaaga ctgtgaggaa gttgtgtcta ttgttgaaga agaaatattt
     7921 gtctcaactg tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt
     7981 gaagatgtat tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg
     8041 gtttgtttga ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga
     8101 gacgtacatc ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata
     8161 taaaatacct cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt
     8221 aaaaccttgt ctatggatag gctacaatta actagacaga aaaattaagg tgtgtagact
     8281 agaagaaaac ataccaggag gaatttttat gtctaagaga acacgaagaa ctttttcaca
     8341 agaattcaag caacaaatcg tcaatcttta cttagctgga aagccacgtg tagaaatcat
     8401 tcgagaatat gaactaac
//
LOCUS       DANJMI010000090         8136 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000090 DANJMI010000000
VERSION     DANJMI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.093"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            92..484
                     /locus_tag="PWU81_002817"
     CDS             92..484
                     /locus_tag="PWU81_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111445.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(657..839)
                     /locus_tag="PWU81_002818"
                     /pseudo
     CDS             complement(657..839)
                     /locus_tag="PWU81_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            826..1023
                     /locus_tag="PWU81_002819"
     CDS             826..1023
                     /locus_tag="PWU81_002819"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111446.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(1066..1641)
                     /locus_tag="PWU81_002820"
     CDS             complement(1066..1641)
                     /locus_tag="PWU81_002820"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL1111447.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1830..2315)
                     /locus_tag="PWU81_002821"
                     /pseudo
     CDS             complement(1830..2315)
                     /locus_tag="PWU81_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
     gene            2772..4283
                     /gene="gadC"
                     /locus_tag="PWU81_002822"
     CDS             2772..4283
                     /gene="gadC"
                     /locus_tag="PWU81_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HDL1111448.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            4296..5696
                     /locus_tag="PWU81_002823"
     CDS             4296..5696
                     /locus_tag="PWU81_002823"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDL1111449.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5861..7296)
                     /locus_tag="PWU81_002824"
                     /pseudo
     CDS             complement(5861..7296)
                     /locus_tag="PWU81_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            7689..>8136
                     /locus_tag="PWU81_002825"
     CDS             7689..>8136
                     /locus_tag="PWU81_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDL1111450.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVS"
BASE COUNT         2594 a         1336 c         1461 g         2745 t
ORIGIN      
        1 ttttttttga cttcatattt ataataaaga cacgtaaatc aattattttt cattttactt
       61 taactaataa tgtttatttg gagggattat tatgttagat gatagattga cgtgttctaa
      121 ttgtggtaca ttattttact atgaggaagt tagaaatatt gtaaaacata gaaaaaaaga
      181 aatgcctata cgttgtccaa attgcaatga agttattaag cgaaagttgt ctcatggtta
      241 ttttgtcagc tacgaactat atgaagaaag taaaataaat ctgaaaaaaa aaccaaaata
      301 ctataaaaat ttgaaaactg gagctattat aactgaaaaa gaatttaaaa atttagtgac
      361 tgaagaagaa agcaggtttt ataatttgtc tggtaatatt tgccctggat ttatggatca
      421 gattattgaa aatgagtcaa ttgaatcaat tgaaaactat gttcctgttg acttaccaaa
      481 atagaaaaaa taatcctgtt tttacagtga attattttaa ttttgatttt caataaaaat
      541 atattacaat aataatcgta atagctaatg aaacaatttc tcaatctttt taccactcca
      601 aacagggagc gtcaataatt ttgtgtaaat aactcgtcct tctgcaaaat aatgggttac
      661 tcagtaaaca ttgaagctaa tgtatccgtc acttgttgga agcctttatg gcttctattt
      721 aagaattttt gattgtatgt atcaaagatg gatactagga aacgctctag tgattcttca
      781 ttttgaaact gctcttttct gcggctgtat ttcttgattt gtttattgaa agattcgatt
      841 gtagaaccag aaggaatgga gaacactttt gaactttaca acaaaactac tgttgataaa
      901 ttaattccaa ttttagttcc aactgattta aatggtcatg atgttttact tcgttttaac
      961 ggatttttag gtgaggatta tagataccta aaaatttctg atcctcaacc tttaacagaa
     1021 taataaaaaa cgtgtaatca attatttgat tacacgtttt ttatttcaat cttcgttgta
     1081 gtagtggttg cgacataccg aaaggtaatc ttcattacct cctactacaa tctgttcacc
     1141 tgaataaact ggaataccat caacaatacg agcattcatt acagcctttt tattgcaaaa
     1201 atggcaaata gttttaattt cctcaatttt atcagcaaat gtcaataatg cttcggatcc
     1261 cttgaagaga ttattttgaa aatcattctt caatccatag caaattactg gtacttcctc
     1321 attcattacg ataagtgata gatcaataat attctctcta cttaaaaatt gagactcatc
     1381 gaccaaaata caatcaactt ttccgtactg atcaataatc tccactaatt ctccgtacac
     1441 atccatttca gaagtaattg caatagctgg tttcgaaaca cctatacgac tctcaacttt
     1501 tccaaatcct gatcgagtat ctattgcgct tgtcattatg ataactctct tcccttgttc
     1561 ttcataatta tgagccactt tcagtagctc aatactcttt ccactattca ttgttccata
     1621 tctgtaaaat aattgtgcca ttaaatttat tcccttcttt tacaattcaa ttattcctta
     1681 attccatctg cttttatgca gctgctcgtt tattccttga tatgtctata acatctacta
     1741 tcccaattta taagccatta gagatggtat tcctgaacat ctctcatatt gttttttgag
     1801 tggtatatca tctgaaaaac tgaaggaatc atgaaaatag cgacatactt atcatttaga
     1861 gttctaactt taaatgcatt tacttcttca gtcagcactt tagtcatgtt ggacatggtt
     1921 tggggcgtgt agtgatgccc atacattttc tcaatcaagt ctgcgatttc cgacatggta
     1981 ataccttttc ggaagaggtg aatgacggtc tcctctaatg tgtcattcgt ccgtctataa
     2041 gcaggaacag tctgttgctt gaactcgcca ttgcggtcgc gcggaatctg gagatgaagt
     2101 tccccatact cggtcttgac cgtacggtca taggagccgt tacgagaatt acccgtgtta
     2161 aaaccaatgc gatcgtactt ttcgtaatcg aggaaagccg ttaactccgt tttgagaagc
     2221 gtattgacag ctttttccaa atggacacgg aataatccat tcaaatcgcc tttgttcgct
     2281 agagtcttta ggatttctgt agtaaaatcg ttcatatgga agtcctcttt tctgtgaatg
     2341 tgttgtggta actctattct acagaaggga cttccttttt tctatttaca caaaatattt
     2401 tacactctca acaaaagtat cttcgattaa tacttgctca agttatgtgc agctacgaaa
     2461 aagtaggtac cgaggtactt tctttttcgc agcttttttt ggacttatga gaagtacatt
     2521 cgctggaaga atacgatata aaacgagctt tttcactaaa gatattaata gagtgaaaga
     2581 aacaagcttt tgttgtcagt tttttcactt ttctaaaaat gaaaataaaa aattctggaa
     2641 gtggagaaga attttgcttg ctatactttg tcttgtagaa aagaggtaga caaaataatg
     2701 caagctcaaa taagcgattt tccataacct tggtaggaat gacaaattaa ataggaaagg
     2761 gaagtaataa tatgaataaa aaaaagttat ctctattcgg tttttttgcc ctgacagctt
     2821 ccatggtttt gactgtttat gagtatccga cctttgcaac atcacaatta catttggttt
     2881 tctttcttct tttgggaggt attttatggt ttctaccagt tgctttatgt gctgcagaaa
     2941 tggcaacagt tgaaggatgg aaagatggtg ggattttcag ttgggttagt caaactttag
     3001 gagaacgttt tggttttgca gctatttttt tccaatggtt tcaaattaca gtaggtttta
     3061 ttacaatgat ctacttcata ctaggagcgc tgtcgtatgt aattaatttt ccagagctta
     3121 atacagatcc atttatcaag tttattggtg tattagtcat tttctgggta ctgacttttt
     3181 cacagttggg cgggaccaat cgtaccgcta aaattgctaa agcaggattt gtcattggaa
     3241 ttgttattcc ttcgatcatt ctctttggtt tagcagcagc ctactttatc ggcggtaatc
     3301 cgattcaaat ggctattaat gcgaaagctt ttattcctga cttttctcag ctttcaactt
     3361 tagtagtatt tgtatctttt atattggctt atatgggcgt agaagcttca gcatcacata
     3421 ttaatgagct ggaaaatccc caaagaaact acccattggc aatgttttta ctagtgattt
     3481 tggccatcgc cttagatgca atcggcggtt tctcagtagc agcggttatt ccacaacatg
     3541 acttgtctct cagtgctggt gtaatccaaa catttgaaac gcttatttta cattttaaca
     3601 gtcatttaca ttggttggta aaagtgattg ctctcatgat tgcctgtggc gttatgggtg
     3661 aagttagttc ttgggtagtt ggtccatcac gagggatgtt tgcagctgct cagcgaggtc
     3721 tgttaccagt tttcttccgg aaaactaata aacaagacgt tccagttccg ttgattatga
     3781 tacaaggaat tatcgttact atttgggcag cagtcttaac ttttggtgga ggaggcaaca
     3841 atttatcatt cttagtagca atctccttaa ctgttgtcat ttatctcgta ggatatttac
     3901 tatttttcct aggttacttt gtattagttt ttaagaaaaa agacttaaaa cgaacttaca
     3961 atgtaccagg aaaaactgtt ggcaaaacga ttatcggtgg aattggtttt attttatcag
     4021 tgttcgcatt gcttatctct tttgtaccac cagcttcaat tgcagctagt gagcaccgaa
     4081 cataccaaat catactactt attagctttg tagtgactgt tcttttacct tttatcattt
     4141 atgaagtaca tgataaaagt gctcacacaa ctattgagga acctactcac gtgaaagctg
     4201 aagacgttaa tcccgctgtt tatccagctg ctagaggaga gcaccacgtt acaaaaaatg
     4261 atgaacatat tttaaaacat taggaggaat tcattatgtt atacggaaaa gataatcaag
     4321 aagaaaaaaa ctatttggaa ccaatttttg gctctgcaag tgaggatgtt gacttgccta
     4381 aatataagtt aaacaaagaa tccattgaac cacgaattgc ttatcaatta gtacaagacg
     4441 agatgttgga tgaaggaaat gcgcgattaa acttagctac tttttgtcaa acgtatatgg
     4501 aacctgaagc agtgaaattg atgacccaaa cgttagaaaa aaatgcaatt gataaatcag
     4561 aatacccacg aacaacggaa attgaaaacc gctgtgtaaa tatgattgct gatttatggc
     4621 atgctccaaa taatgaaaaa ttcatgggaa cttcaacgat cggctcttca gaagcctgca
     4681 tgctgggtgg tatggccatg aaatttgctt ggcgtaaacg tgctgaaaaa ttaggtcttg
     4741 atattcaagc aaaaaaacct aacctggtga tctcttctgg ttaccaagtt tgttgggaaa
     4801 aattctgtgt atattgggat gtggaactga gagaagtccc aatggatgaa aaacatatgt
     4861 caattaatct agatactgtc atggattatg tggatgagta cacaattggt attgtaggta
     4921 ttatgggtat tacttacact ggtcgttatg atgatatcaa gggtctgaat gatttagttg
     4981 aagctcacaa taaacaaact gactataaag tatacattca tgttgacgct gcatcgggtg
     5041 gcttttatgc accatttact gaacctgatc tagtttggga ttttcaattg aaaaatgtta
     5101 tctcaattaa ttcttcaggt cacaaatatg gtttggtata tccaggtgtg ggttgggtct
     5161 tatggcgtga ccaacaatac ttaccagaag aattagtatt taaagttagt tacttaggtg
     5221 gagaaatgcc aactatggct atcaacttct ctcatagtgc agcacaacta attgggcaat
     5281 actacaactt tgtacgctat ggctttgatg gttatcgtga tattcaccaa agaactcatg
     5341 atgttgctgt ttatttagcc aaagagatcg aaaaaactgg tatttttgaa atcattaatg
     5401 atggatcaga attgccagtt gtgtgctata agctgaaaga agatcccaat cgcgaatgga
     5461 cactatatga tttatctgat cgtctgttaa tgaagggatg gcaagtccca gcctacccat
     5521 tacctaaaga cttggatcaa ttaattattc aacgcttagt tgttcgagca gactttggaa
     5581 tgaacatggc tggtgattat gtacaagata tgaaccaagc aattgaagag ttgaataaag
     5641 ctcatattgt ttatcataaa aaacaggatg tgaaaacgta cggctttact cactaaaatt
     5701 cttcagagaa atataaaaga agttaattaa ctaataaaag gagttgtgat taccacacag
     5761 ctccttaaat ctatttgaat aaatatatga tattcatttg ttacactctc aatcttagct
     5821 gttgttctat tcatcattac tcttattgta agtagttcag tcaaatttgc ttgttcaatt
     5881 gatcgataac agtatgaatt gtttgaaaag taatcggata tataattgtt ttctcactac
     5941 ctaagccttc ttggtataaa gaactatcgc aaagtataat ccgctgagta caaaagaaag
     6001 tgtcgtctac tatttctcta agttgtttac aaatgatatt actaatagaa aagtgttctc
     6061 ccaaaatgtc ttctaactgt tgtcgatagt ataaatacct aaagtctgac ggagctacta
     6121 tgaacacttc aattggagat ttccgtttat gctcatttaa aagacttaaa ctaatggtta
     6181 gtttacgaat agaattctca ttccaacgca gaaataaatt gttttgtttt cgccattgtt
     6241 caagaatttc atgaacttct aagtacaaag cgcgttgctc ttcatttaac aagtaagtct
     6301 tatcaggttg aaatatctga atatcagccc aaagtgagcg gataaacggg agaagtgctt
     6361 tatcaaaaag tatctgattt tcttcagata tattcagaga taagataagt ttttgactga
     6421 gctcaatcac caaagggtta tctgctacat ggttttgata aacaacgttc aagtcttttt
     6481 gtaacaattc aaagttatcg catagataat ttctagagtt gaatagaaag tagatataaa
     6541 ggcattcatc ttcgtcatga cataaagaga aagaaagttg ctctaatcct ttctgtatta
     6601 gctgaaacaa gggagtatgt cgggcaactc ttttttcttc ctgactaaaa tatattgggt
     6661 tagtcttaga tctctgcaaa ccgatagcga tacctaataa aataaattgt ttttcaactg
     6721 tagggtaact tcttgaaaaa aatgtttttc tacataatca atcaattgtt gaccgctttg
     6781 aataattgat ttatttagct ggatcaactc tgtccgattt gtctcataga ttatcgttag
     6841 taacaaactt cgataatcct tttcaggaat ttcaaaatgg ccatcagcat ttaagtactg
     6901 catttgttta aaaaaattaa tcaactcatt tcgtaagttg taaactttgc ttaacgaaat
     6961 gaaggtagta tcagctatat tactccaatt gtgttcccct gataaataat gtcctagtac
     7021 attgaaaaag tcagagcgct gatagatgag ttgagtgagt gccaacctcg aaaacgaaga
     7081 gtcgaatgcg actgtaaagc tacttctaga tttctccact gaaataattt gctgatggag
     7141 tatgtcggta atgcattcca agtcgttagt gacagtgagt gggcaaacgt tcaattgatc
     7201 tgctatttcg cgaatctgaa tggtcccttt ttcgtgtaat tgctcacaaa tgtaaacttg
     7261 acgaaaaata cttttctcca gataggtttc caacatagta ctcctcctag tcattttata
     7321 cataactatt ataactctac caatcggaat agtcctgtaa tatattgaat caaaaatata
     7381 aattataaaa taattagatg gggtatttgg tttaagtatc ccttatatag taataaattt
     7441 ataggtgtga ttctcgaatt aatacccgaa aatgtgagag aatttttatg gattttttag
     7501 gatacaaatt aattattttt gcttttatcc tgattaccag tccagtactg tcggtatccc
     7561 attttgcagc aaaatcatgt tgataaagtg acaagaagac tgatgagata tattatccaa
     7621 attgagaaga taattaatcg gattcttgag tataccctac tttttaagaa agtagggtat
     7681 acaagcgtat gaataaagac ataactttcc ctgtgatgga cgttcggctt gttccaatta
     7741 ccaaaataca ggcgaacgat tataatccca atcgagtagc gtctccggaa atgaacttat
     7801 tggagctttc tatcattgaa gatggcttta ctcaaccatt agtttgttat tacaaggaaa
     7861 aagaagagaa gtatatcttg gttgatgtgc ttcataggta tcgtgttgct aaggaaagac
     7921 tcaacttaaa agttgtaccg gtaacagtta ttgataaacc catagaagaa cgtatggctt
     7981 caacaattcg acacaatcgt gcaagaggga cccatgaaat aagaggaatg gaaattgtca
     8041 tccaaagatt agtaaaaaat ggatggtcag acaagagaat atccaaagaa ttaggcatgg
     8101 acattgaaga aatttttcgc tttaagcaag tgtctg
//
LOCUS       DANJMI010000091         8128 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000091 DANJMI010000000
VERSION     DANJMI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.094"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(26..1378)
                     /gene="celB"
                     /locus_tag="PWU81_002826"
     CDS             complement(26..1378)
                     /gene="celB"
                     /locus_tag="PWU81_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL1111451.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1378..1896)
                     /locus_tag="PWU81_002827"
     CDS             complement(1378..1896)
                     /locus_tag="PWU81_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111452.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1912..2238)
                     /locus_tag="PWU81_002828"
     CDS             complement(1912..2238)
                     /locus_tag="PWU81_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HDL1111453.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2251..4230)
                     /locus_tag="PWU81_002829"
     CDS             complement(2251..4230)
                     /locus_tag="PWU81_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL1111454.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4251..4565)
                     /locus_tag="PWU81_002830"
     CDS             complement(4251..4565)
                     /locus_tag="PWU81_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HDL1111455.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4733..5026
                     /locus_tag="PWU81_002831"
     CDS             4733..5026
                     /locus_tag="PWU81_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDL1111456.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5104..6216)
                     /locus_tag="PWU81_002832"
     CDS             complement(5104..6216)
                     /locus_tag="PWU81_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL1111457.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6369..6593
                     /locus_tag="PWU81_002833"
     CDS             6369..6593
                     /locus_tag="PWU81_002833"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111458.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            6603..6782
                     /locus_tag="PWU81_002834"
     CDS             6603..6782
                     /locus_tag="PWU81_002834"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111459.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            6976..7146
                     /locus_tag="PWU81_002835"
     CDS             6976..7146
                     /locus_tag="PWU81_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1111460.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(7570..7953)
                     /locus_tag="PWU81_002836"
                     /pseudo
     CDS             complement(7570..7953)
                     /locus_tag="PWU81_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(7899..>8128)
                     /locus_tag="PWU81_002837"
     CDS             complement(7899..>8128)
                     /locus_tag="PWU81_002837"
                     /inference="COORDINATES: protein motif:HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111461.1"
                     /translation="YQLVETQAPIGYELDATPIEFEIERSQTAVVELTKENRLTPGGV
                     VLTKIDDQSGEILQELFLSYRTEKEKRYKPV"
BASE COUNT         2556 a         1642 c         1322 g         2608 t
ORIGIN      
        1 gtcacagttc cttttttctt agaaatcatt ttaacttttc actagctaat tctgctgcct
       61 tttctctttc ttcatttaat aatttattat catagaattt gataaatggg aaccagataa
      121 tgaatgcgat gattgcacaa atgactgcta acacagcagc tttccaatca ccaccactac
      181 caacaaatgc tccgatcccg actggtgaag gccacggcat ctgtgcaagc atcggtttga
      241 cgatttccag ccgaatggca aaataagcga gtgtagcaga agccatcgga gctaagaaaa
      301 acggaaccgc taaataggga ttgtagacga ttggcatccc gaaaatgatt ggttcattga
      361 tattgaataa tcctggtaca agtgaggctt ttcctaacac tttcagctga tcagatttcg
      421 cgaggaatgc aataaagatg atcaatccta aagtcgcacc tgatccacca acggttacat
      481 aagaattgtt gaattctcct gccaacggat aatttgcgcc ttgtgcgtta gctgccatat
      541 tagctaatac gatcggggtc aagaaagaag tgatgatatt cgctccatgg atccctacga
      601 tccacaatgc atggatcaag aaataaatca ccatgacacc aagccatgta ttcgtcaact
      661 gcgtaacaaa cccaaatggg atagcaatca ttttgaagat atccgttcct agtgcaacta
      721 ataatccatt gatgatcatc accacaaacg caatgacaaa tgttgggatc agagcagtga
      781 aagaacgaga aactcctggc ggaacaacat ctggcatttt tatgacccaa tttctcttga
      841 cacacataca atacaattcg accgcaataa tcgacataat aatagctgta aagattcctg
      901 aagttcctaa acgttcgaca aaagagccca tcctaatacc atttactaca gtttctgtct
      961 cattgataga agcaatcaag ctgattttac catcagtcat gatcaattca gggatacaca
     1021 taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg attaacattt aatttttgtt
     1081 catctcgttc aattgacgtt aattcataag caaacacaat ggcaaagtaa atagataaaa
     1141 tccccatcgt cattgtattg acgatcatgt acaagtcact aactttgaaa aaagttgcat
     1201 taaaaaatcc ttctaaaaat gggaacggta atggcaaaat atttaaaacc aaaaacatcg
     1261 agcctacaat cgtgaaagga atagaagcca tccccgctgc catgacagcc cgaacgattt
     1321 taagttgcgc aattttcccc ataggtccca tcaggtattt ctgcaaaaaa tcaaacatta
     1381 gttattctcc tttcctaaat ataacgcttt ttcatattgt ttaatacgga tatactgttg
     1441 gtcttggttg agtgaaattt tctttaaacg atactgtata aatgcactgg caaaaatacc
     1501 gcttacaaca agaatgaaaa caaataactg tcctccattg ttatttgcca taaaaggata
     1561 caagaatgta aatcctgagg taaacaacac tggaatcact aataaattaa agacactctg
     1621 gaacaaaaaa taccactttg tccatggaac gttcgtttgg tgttttcgat aaagctttac
     1681 ttgttctcct actgcaggca aaatagctaa aatcagaatc atcggtaaac tacttcctaa
     1741 acttttagct gatagaagca gaatcatcca ttgcatgttc atgaaaaaga aaaaggcagt
     1801 caggtaacgt acgattagat aacgattgaa atacatgttt ttcaaactaa gttgcttttt
     1861 ttctaattcg attttccttt ttactgtatc ctgcacaatg atccttcctt tctatttatt
     1921 tttttgatac aaataattca tttcctgtgc aacttccaat aatgtcatcg tcgtcatcag
     1981 atgatcttgt gcatggacca tgataatttc catttctatt tttgtcccgc tggcatattc
     2041 tttcagaagt tgagtttgtg actgatgcgc atgaagaatc tcttcatttg cttgtcgcaa
     2101 cttctcttct acttcttcaa acatctcttt acgcatcgag cgaaatgctt catgtatcag
     2161 tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa gtcacttgta gtgtttcact
     2221 gtttattttt tcagccatct gttttcctcc ttattcgatc agtttgataa atttactgct
     2281 gaaattctca aaaataggtt cagctaagat ttcctgctgt acttttagct ggtcaactaa
     2341 cctaacgatt gctttagtca cgaccgtaat tccttcattt tcgacataag aaggcgaaac
     2401 taaaaaaaca aaatgaattt cttgatattg atcccactgc atcccagaag gaatcaatgc
     2461 aacggctacc tttgtttcga ttccaactgg aacagcggga tgtgggactg ccactgtatc
     2521 actgaatatg atttgtccca tattttcacg ttgctggatc tgctttcgca tttctcttat
     2581 atatccttct tcttcattta tgctcagctt ttgcagcaaa tcatgaagaa tttcttcctt
     2641 tttcggttcg tacgtataag tcttgaaaaa tttttcagac aactgttcgg tgtaaatctg
     2701 ctttttttgc tgtagagaaa atgctggtat cggtctatct gaggaatgat agggtataga
     2761 ttctccaact acctttcgta ttttacgaac atcatcttca ttcaaaaaaa tactgacatg
     2821 taaaactgga attttgaaaa acattgtcga aagatcaata gaagaaataa tcaggtcgat
     2881 tccttttaaa gttgtttcat tgatctcata atagcctttt acacttacca ctgtaatatt
     2941 tttaccgaat tcactgacta cgcgattctt taatagttgg gcactgccgt aacccgttgc
     3001 acagataatc aatgcatgta gtttatgcgc agctctttct ttttccaatg ccgccatgag
     3061 atgcaaagcc aaataggccc attcatcttc attgatggta tagtttttca aagccggcat
     3121 gttgctgaaa tgccttttta ctgcgtcaaa gactttggga ttttctttct ttatctcctc
     3181 agtcaatggg ttctcaatgc gaatacctcg gctcaatcga atcatcatcg gtgacaaatg
     3241 atcgagtagt ccattgaaaa gctggagatc ctctactaat gaaaaatgaa attcctgaga
     3301 caatcttttc agaactttcg ctaattcctt tgatagttcc tgatcatttt gattttcttt
     3361 gtgattcgac ttagccatga gatgtaatgt caaataagca atttcttctt cgggaaacga
     3421 tacacctgcg attacctcaa tacgttgaac gatccgctgc gccacttgat actctactcg
     3481 cttagaaact tcttgttcga tgcctaattc cttgatttca aacccttctc ggagccgttt
     3541 gatccccaaa gccaagtgta aagtcagatt ctgtatgata aaatccgaaa cttttaactt
     3601 agcttctctt gtttcatcga gaatgataat gactagctct tcaaaactga tgtctgggaa
     3661 aaaacggctt tgccctaaat attctttcaa tgaatttcca taatggtttc caaaaaaagt
     3721 atccatgata aaatgccgtc gatttctttc ttccccttcg atccatgtcc cttgaccatg
     3781 cttagaaacg atttttagtg aatatggaat caatcgctcc ttgatttctt gcatgtcctt
     3841 tgtcaaactg gaacgactga tataaagctc ttctgccaaa tcatccataa aaagaatggc
     3901 atcttccaaa agcaattggt tcaatatata cttttgtcga tcttcggact cataaaatcc
     3961 ttgcggttct ctagcatatc caggtactat ttcttgccga ctcaaaaaca agtcaaatgt
     4021 cagtctttgt aaaatatgca gttggtagcc ataacctggt ttagcaatga tcttacctcc
     4081 gtttttttct actaactctt tgatccgctg caagtaatta cggattgtcc gatcagataa
     4141 taacaattca gaagctaaac gttgactggt gacatactcc ttcttgtgat caatcaaata
     4201 tctcaacagc aatttttcct tctgtttcat tatcatccac tcctattcca tcatatgttc
     4261 tcttcgatca cctctgctaa cttctggatg cctgtaggga ttgggacata cgcttggaaa
     4321 gggatactaa caacaggctt tccaacagat tctcctaatt ttttgaattt atccaaaaac
     4381 atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac tattcttaat catattatcg
     4441 ccttctgtag ctgaaactgc atctacgagt acctcttctc ttttttgctt aaagaaatcc
     4501 gttgtctttg aggccatcat cgaggatgac attcctgccg cacaaataat caatgctttt
     4561 ttcataaatc aatcactcct tttattttta taaaccaagc atatcttgta agcgttttct
     4621 attcaaaata tatatttccg tagcattgcg gaaaaaatgt gtatcatatc ttgaataata
     4681 atttctggca ccgtgatata ctagtgatag aattttttca ggaggggatt tagtgagtat
     4741 cacagtgaat attttgtata caagtacaaa tggacaagcc agaaaatttg cagaagaaat
     4801 ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa ggaaacgaga aatatgacta
     4861 ttactttcca ctagaagatc cagaatctct tttactgatc gaccgttgga aagatgaaga
     4921 agcaatcgaa aagcatcaca aatccgaaat gatggctcaa ataaacgaac tccgtaaaaa
     4981 gtataaatta aaaatgaaag tcgaacgttt cactgattta ccgtaaaaag atacacatgg
     5041 aatgatagca atttatcatc catgtgtatc ttcacttttt ccttattttt tattcgatct
     5101 catctaatga ttcaaaccgt gtttttctct tatttttcgg ataagtattt cagtcacccc
     5161 aatatacatg attcctaaac ataccccaag gatattctca aaaatgcgga tcgttactgc
     5221 tcctggaacc ccgaataaac tggcagtgat tgccaaagca ccaaaagaat taaaaactgt
     5281 tttatatcta taggtagaac aaacacctaa agctagacca cctaatatac cgacccagtt
     5341 aaaaggaata aattggctga taccaataaa taaaacacat ccaatcaatg agccaatgat
     5401 tcggtctact gctcgttctt tgaaaccaat cgacatcaat ccatagctag atacaataga
     5461 agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg acatactctc ctgcaacgat
     5521 caaaagacta atgccaaatg catagatgag catccaaatt ttttcctgcg aatacatgcc
     5581 tttcaaaaaa atctctttaa acaagctttt gtctttgttc ttttctcgat gttttctgta
     5641 aaggagaact gaaaaccaac aaaaaaacaa aaataataag ctacttgttt gtgtaaagaa
     5701 attcttgttc aacgaatctt taggtaacga ataaacaata aagagatatg aaaagccgta
     5761 caaagaagca ttccccattt tgggatcact agcggttgcc attaatatgc tgcttagtaa
     5821 aataaaatga atcaacatgc ccataactga ccatttgatc agctggacat acggtgctac
     5881 taatagtgag aacatgacga ttgctaaacc tatgatcgaa tgggaaatct tataaccaaa
     5941 gtcaacaaaa cgaatactca tcaacataca gaataaaacc acagcgtatg gtttatgctt
     6001 cgattcaaat aatgttgtaa ttataatgat aaaacaaatg gcaaacaata cgatgaataa
     6061 tgatcgtaaa aacaatgaac aaatatacat gcacttttct tttttagaag ctgcttctcg
     6121 tattttttgt tttaaaataa acggatctag ttgtagagac tgataaaaat ccacctgttg
     6181 tcgtcctttc tttttacttt tattttttac agtcatctat gacaggttgt aaatactatg
     6241 gaacatcata tggcgttgtc attttcttgt caactatttt tcctattcat gtttttataa
     6301 aaaaacatat cgttttcttg tatgattaaa tcaccacaaa ataaacaact gagagaacga
     6361 tatgctttat gaatggttct atcaaaaaaa ttctgagatc aatagacaaa aatttgatga
     6421 ttacagaggt ctcttacgaa accttacaga agaaaaaaac attgatcgtt gctgcagttc
     6481 tcttccctgc tccatgtgtc tgtagaaatt gtggttctgc tgtggtagat ggaaatagga
     6541 aattaatggt ggtgaaaaac aagaagaagg aaaccattgt ccactttgaa taatataatc
     6601 atatgccaat ggttatacgc ctaaaaaagc agcgctatac ctgtaaaaac tgtcgaagcc
     6661 attggaacgc tcaaagttat tttatccgtc caaggcactc gatttcaaat catgtcagac
     6721 ataaaatcac ttctttactg actgaaaaag tgtcgttatt ttttatttcg aaaagttgtt
     6781 aggtatcttt gacaaccgtt attcgcacat tgaaagagtt gagaagctat ttaccaaagt
     6841 cttccaaaag aattctgtct tgagtgttga gggttgtgaa tttcgttctc acgtttccgt
     6901 tgaggataaa ctgagtttta tttccgcgga tggcgagaca gaaaaattaa tcgagaaaat
     6961 ttacctacac aaaaaattcc tcgattaaca aactatttcc ttagctgcac taatcctaaa
     7021 gaaatagaat tcttagttac agacatgaac gccacctact ttcagctcac caaacatgtt
     7081 ctaccaaatg cgaaattggt gattgatcga ttacttggtt tttccacgcc tttcaaggag
     7141 gcgtaacctt tattcacgag ttggttgaag ctttccgaga caaaagaccc gacttgtttt
     7201 tctccttatt gacaaagagc cattaaaact agagaaaaaa ttacaactga tcgctgatta
     7261 tttgtagaaa gaaaatcaaa gtattaccca ataaacttga gtcattaggc aaaaaacact
     7321 gcgaaactca agaatgattt tcgcagtgtt tttgtatatt ttttattaac tagtattact
     7381 acaaaggttt atttttcact tctcaacgca gcacaaaatt gtctttactt atttactggt
     7441 tgggttcaac cttctctact ccgatactaa tatttgtttg tatacccctt ctaaaatcgg
     7501 tagtttccat tcaattgatg ggtactagtt gattttaaaa actatgctta ttcaattttt
     7561 tattttagct catatatttc gtttctttct aaattttata ctgatgaaaa caagtagcac
     7621 acccaatatt gacaatataa actggctaat gatggtttct ccagtttttg gaagatgctt
     7681 atttccactg cttgttggat tgtttcctct tatatctgag ctatcagcaa cttttctatt
     7741 ctcttttgtt aaaaagatcg ccacttcttg ttctttttcg atcttgaaag ttactggtgt
     7801 cgcatccaat tcatacccta ttggcgcttg agtttctacc agctgatatg ctcctggtgc
     7861 taaaccgtca attgccagtt ttccatcttc tcctgtagtt agaccggttt gtagcgtttc
     7921 tccttctcgg ttctgtaact caaaaacagc tcctgcagga tttccccgct ttggtcatcg
     7981 atctttgtca acacgacacc gcctggtgta agtcggtttt ctttcgttaa ttctaccacc
     8041 gctgtctgac ttctttcgat ctcaaactcg attggtgtcg catctaattc ataccctatt
     8101 ggcgcttgag tttctaccag ctgatatg
//
LOCUS       DANJMI010000092         7618 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000092 DANJMI010000000
VERSION     DANJMI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.095"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(<1..1251)
                     /locus_tag="PWU81_002838"
     CDS             complement(<1..1251)
                     /locus_tag="PWU81_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111462.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENK"
     gene            complement(1271..1438)
                     /locus_tag="PWU81_002839"
     CDS             complement(1271..1438)
                     /locus_tag="PWU81_002839"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111463.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            complement(1475..2164)
                     /locus_tag="PWU81_002840"
     CDS             complement(1475..2164)
                     /locus_tag="PWU81_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HDL1111464.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            complement(2419..2739)
                     /locus_tag="PWU81_002841"
     CDS             complement(2419..2739)
                     /locus_tag="PWU81_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111465.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            complement(2772..3890)
                     /locus_tag="PWU81_002842"
     CDS             complement(2772..3890)
                     /locus_tag="PWU81_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111466.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            complement(3919..4713)
                     /locus_tag="PWU81_002843"
     CDS             complement(3919..4713)
                     /locus_tag="PWU81_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111467.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            complement(4723..5598)
                     /locus_tag="PWU81_002844"
     CDS             complement(4723..5598)
                     /locus_tag="PWU81_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111468.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            complement(5815..6072)
                     /locus_tag="PWU81_002845"
     CDS             complement(5815..6072)
                     /locus_tag="PWU81_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111469.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            complement(6075..6299)
                     /locus_tag="PWU81_002846"
     CDS             complement(6075..6299)
                     /locus_tag="PWU81_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111470.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            complement(6472..7515)
                     /locus_tag="PWU81_002847"
     CDS             complement(6472..7515)
                     /locus_tag="PWU81_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL1111471.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
BASE COUNT         2123 a         1586 c          962 g         2947 t
ORIGIN      
        1 tttattttca aacctcagct gttcaagttc ttttctgatt ccttttaatt ctttaatctc
       61 ttgcttcaaa agttcctctt ctttttttgc cgatttgact aaagattcta accgaccgtt
      121 ttctttactc gcaatactta attcttttct taatttgatt acttcagatt tgttttcttt
      181 tctaatactt gcaatttgtt ttgctatttc ataatcagaa ttcaggatca attcttcctt
      241 agcattttta aacaaccgag ttaaaacgaa ctgatataac aaactataaa aaactagcaa
      301 atagtcaatt tgtcggtact cggtattctg atcaattgca tatagagaga ttaaaacgaa
      361 atacacaata tcattcagct cgctaccctt gatctcaaaa ttattgacta agtaactatc
      421 aatacccatc aaatctgcaa aatctccata caaagaaaaa tatgattttg acaatatatc
      481 tatatctgaa tcgtcaaacc catttactat tttcccatca ttgttagtag ttgctaacga
      541 agcaatagtg acttttgaat tgggttttac aaatcctata cctaatagtt ctttcatctt
      601 tttaatttca agtttgttat ccttttgttc ggctttatgc tgtttcatat tgtaaagcgg
      661 ataaaatgct gttccgtaag caccttttag tacgttatct ataaaataat cttcagaacc
      721 tgttttgaat agaaagttaa tcgcagccaa attagataac agctcttcag tgttgtgcat
      781 caatttttta tcatttaata aattatcccc aagatatttc attgcataat aagggtttgt
      841 ttcgctactt atttttaata ttttttgata gctattcctc acgatattaa ccaaaatatc
      901 tgtctgatta attgaattac agtacaaagc cactcctaat gccatataaa cattcaaatc
      961 atgttttgcc gtaccgttga ataaaacgtc ttcaactttt gaattatctc gtctatactt
     1021 tttgatcata tttttgatat tttgatcatt ctcttggtat atttttttca ctcgttccaa
     1081 tgccttgggt ttgttggaaa cagcaacagc gaagtctagt tctttgaaat caatatttag
     1141 tatctgtacc tcatcaccta gattataatc tctatttacg ctcggaaaac tgctgaaatc
     1201 taactgtttt ttacctattt ttggcgaaaa tattccttcg atttctggca aatgtaatca
     1261 cactctctcc ttatttatct ttagaaaatt tgtcactatt ctccttatat ttatcaataa
     1321 acctttttaa tcgagcaata taagcgttaa cccttttagg ttcttctttt cgccattcat
     1381 cgtttacgtg accattttca aagaaagaag ctaaaagagt ttccacttca ttaaccatat
     1441 ctgcgatcgt atatttttct gtaccctcac tcaattattc atgaactgtt aagtgttcat
     1501 agctaagttc attcataatt ttgttatagc gttttcttgc tttttcaaat tctttctgtt
     1561 cgtcaaattc cacttgattc ttcatgttgt tcttcctctc tgtttgatat tttttcactt
     1621 cttcaagttt agcatctagt tccgctttaa cttctgtcac ttctttaagc gcttgggata
     1681 acttatgaga caatttcgca taagtttctc tcttgtattc atttcctact cccaactctt
     1741 cggagtagaa ctcttctagt agtcttgcaa aattaacatt tctcctgcca gcatagtttt
     1801 ttagcttctc aaaatcagat tgaaagacag caacactttt gtaatccttt tcaccctcaa
     1861 aatcaaaagc atagtcatac tttcctgaaa tcaattcatc aataactgta gcaatatatt
     1921 gagagataga aatattatct tcacttctat gtcgctctat aagattatat atatcagggc
     1981 tgacatgaaa cgtacttctc ttttttgttt gcctttttct aactgttacg accacttttg
     2041 gaacttcact atctccattg atctctttgt atttttcaat aaataatttt ctagcttgtt
     2101 ctcgactttt tgccggaatt tcatctacaa ttttatcttc taaataaata tagtgtgttc
     2161 tcatacattc gtcctctatc ttctttactc tcaatcttca tgatcttagt tcttaccttg
     2221 tttactctta aacattgcaa aaaaatctag tcaaaatcaa acttgaaatc atcgtctatc
     2281 acaacaccct taacttcatc tttagatttc ccaaaggaac ccttattttc aaatccttta
     2341 tttttgaatg atgatggtta tggaaaactt cctttgaaat tctgacaagc ataactagtc
     2401 agaactgttt tacttagatt aagctttcaa aagatactga tgaagaacta agttctcagt
     2461 accttcccat tcttcaataa aaaatatttc tccttctttt acccactcta tagctaattt
     2521 cttataatca aataaaccat atgtgtttaa gcctttgtct ttcattaaac tcaaaaactg
     2581 atcccctttt aatctttctg tatcatccat cagaaattca actaaatctt tatccatgcg
     2641 tgcaactttt tcgtctttta gtgccattga tttagcatag tcagttgaat cttttagaat
     2701 aataacacct acatttcctt ccacaatttt cttcttcata tttacctcct accatctttt
     2761 ccaaagagta tttagtcaaa atcaaactcg aaatcatcgt ctatcacaac acccttaact
     2821 tcatctttag atttcccaaa ggagccctta ttttcaaatc ctttgttttt aaattctaaa
     2881 gctttttcga cttcttcttt ggatattgta tgaacttttt tcttaactac tccaagttta
     2941 tacagctctt caaactctgg tacttcggat ctgaaataca atccatcagt acgaattttt
     3001 aatagcccat atgcgctatg aacgtatttc tgttcatatc taaccatacg atctctaaat
     3061 acgcctgcta ccatggagga atacgctcga ttatacatac tgtatttatc gaaggtgctt
     3121 gtttctcctt ctcttaatga tttttgtccg atagcaccaa atactttcgt tctaagattt
     3181 ttataaagct tcaacgtatc aggatcagtt tttaatggct cgattctttc aaaatattct
     3241 ttgagtaatc cttttttcgt caaaaatgtg tggctatcta ggacttccat ctctttaata
     3301 tcataataat caaggataag tgttagctca atctccgata accaaatagg ctcattagct
     3361 gttttgggat tttcgccata atccgcaaac atcgactgtg attttggatt agcatatgtt
     3421 ccttctttgg ctgttccttc aaaattaacc aaaaagaaca tatacgtttt taaattttca
     3481 tacggtctta cttttggtct atttatcgct ctcaaaatag cttgatctgt attatatttt
     3541 gctttcaaat aagtgtttga cggcaaagga taatttctca tgatcctcga ataggcttcg
     3601 ttgaaatcat attcgatcat ctttgctcct tcctcaaaca aatccaagtt atacatgcta
     3661 ccggaggtac tggctctata ggctgaactt ggaacaaaga aaggtgcatc ttccggctta
     3721 tcatagccca aaaaatgtat tcctgtttgc ttgttctgac ggtaaatgtc catcaaaaga
     3781 tatttgatcg tactaaaaga atctaatttt cctcttgtca tccaccctaa tccgacaagt
     3841 cttttaattt tattctgcat ttcaggtgtg tatatgctat tgtttcccat ttatctgctc
     3901 atctccggct tttttattct agaccctata tttgttcatt acaccactta tctcgtctac
     3961 aaaggctccc agatcgtgtg taaaggcatc atagtaatct tcaatctcac tcgattcagt
     4021 tctccggttt cttccgtttt tatttctgaa tagatacatc tgcgtttctc ttaaatccct
     4081 tgcttttttc gcaagtgcta ataaatccgt aaaattatca ataaccataa tgtcattgat
     4141 cataaaacct tcttcatgac catacttttc agccatctcg ataaaatcat taacatcgaa
     4201 caactccaat aattctgctt tatatttctc taacaatgta tttaaatagc gatttctcaa
     4261 ttcccatgaa ctttcaccag ttccgtattc aaccttgatc cctaaattct tagtgtagcc
     4321 ttttgctgtg tatcgtccaa tgaagtctcc cttattccga ttgctatctt cttctttgaa
     4381 aaactcacgc caaatcccct ttttcttctt aggaattaga cctctgctct tgaaatcatc
     4441 atattttttt gaagcttctc ttcgtgtatc taagtacctt tgcttcagct ctgtagctga
     4501 ttcattcgtt ccatattcaa cagtgatcct taagcgcctg ctataacctt ttgctgtata
     4561 tcgtccaaca aagtcaccgc cacttcgatc actactgact tccttaacaa attctctcca
     4621 tatacctttt ttgtcctttg gtattaatcc tcgtcttttc atatcttgat atttttcaga
     4681 agcttctttt agagtattct tttttgctac catatattcc actcactctt tgttatctag
     4741 tattttaata actggtttca tttttgtaat atcttctttt tcagctatat acaactccgt
     4801 tacggtaacg atcttagtat gatttagatt aattactttc acttgatcgc caattttgat
     4861 ttttcctcgc aaccgtttgg gaataaacca gttgtataat cgtgatttat tattatctac
     4921 ataagctttt attgcatttt caggaaacag gttatttagt ctttcaattt ttttactttt
     4981 aaaaaattca ttcgcaaact tttcatcaac aactaaatta aataaagttt cagaatttac
     5041 agcttccata tctattcctt tgtagatagg agaaagcaat tttttatatt ttgccagtaa
     5101 tgactttcca gaattactaa tcttaatttg gttacttaaa tcttcttgat aaagaacccc
     5161 atatcgaagt aacgccttaa tataatgttt taatatgccg ttgataccag aataaatatc
     5221 catgccgttc tcaatatagt agaaagtgtc gtctctccaa aacaatttga agctattatt
     5281 caaataaagt aacaagatca atatatgttt gtcagaaatt ctgacaaatc tattactttt
     5341 tagatactca ccaatggctt cacttaactc tttgaagcca ttgtattttc ccgataaaaa
     5401 tgtcgtaaca ttatcttctc tctttttcct atggtgtgaa cttaccctga aataatagag
     5461 tctattctca tttaatattg aaaaataaat actttcagtt gtttgtgagc attgtatata
     5521 gttgaaatga tatccatctg gcatgacttt tttaactgta ttttgtacta gctctgacca
     5581 ccttttgtta gtagtcatat atttttttcc attaaaccaa acataaatat cttttttaaa
     5641 aattttgttc cccccctatt gcgttttctt tgacattaaa gccctttgtt ttcatcacat
     5701 tcgtatagcc ctagccactc ataacctgat gcttttgcga agtttattta aaagtgtttt
     5761 cctttgtaat ctacctttcc cctcgtcctg gtacttttct tggcgactat taaactattc
     5821 aactttttta gaatcattgt cagcttgaaa tccttcacgt ttcattatgt cttctgcttt
     5881 tccaatgctg attcctttcc aatcacgcca tcctacgctt cccttatttt gacgataggc
     5941 tttaatagct gattcggata tttgccatct tgtagccaac tctttggctg tgtacttatt
     6001 tgaaaaaagt agcttctcta tcagtgaaat atcaatcatt ttattctcac tcctgaaatc
     6061 ttcattaatc atctctagct agctatatct tcaatcaatt ctaatatgtc ggataaacca
     6121 tcaacagctt cttcaagttg atcaacggat tcttctgctt tttcatatct ttctgatccc
     6181 tgtagatttt taggtatatt ttctttgtac tcttcttctt gaattacttc taactcatcc
     6241 tttagctcgg aaagtcgctc gcttatttct tctaaccttt ttcttcttct ttggttcatc
     6301 aaaatcacct cttcgcataa tctctacgat tttaatatac cacttttgtg tactacattc
     6361 aatatttttt gttatagatt cgtattacaa aaatttcctc taaaaaaata agagcctatt
     6421 taccatttca gtaattaagc tcttatttca tttagttgat aattctatca attaacgtac
     6481 agcaaccgcc attccagtca cattaaatgc tctctcatac gtgcttgcat tgatccattt
     6541 tgcgccaaat acagggtcaa caacatggta tgcaccattt ctgaagccat ccacgagaac
     6601 tacatgtcca ttccatactg cacgacccca aggcatttgt ttgaactctg gcgtagcata
     6661 gttataagta atccaagtta caaccggatt accattgcga atttcattct tgattccatt
     6721 cgttcctgaa ccagtgatat tgacggcatt accgccaacg ttttttgccc atttaactac
     6781 tgggtcagcc atcatgcctt gatacgttcc attaacgtta tgacgccact ctcctgagta
     6841 accatgataa ggattattgt cattagcttt tggcatatag tttacaaaac tggttaagct
     6901 ttgatttgtg gctacacctt tgtagtgtaa accttctaat aaagaagctc cttcgcataa
     6961 cattgtattt ccttgccata caacaggaac gttcaacact ttttcagtgc ttggagcagg
     7021 tgtaggattt ggtacgctgt ttggcattgc ataccagcct gtaccttcaa tcttccaacc
     7081 ttgtttacta attacgttct tttcattttg atctaacgtg tagtgatgcc aattcacgta
     7141 tttgttatat aaacgtgtta caggaactct atttgctccg ttgtaggaat agaacgcaac
     7201 accttcgtac gtccatcctg tgtgtgaaat cttatctctt tctccggcat caactgtgta
     7261 aaaatgttca ccgttatacc gattatacaa acgataaaca ggagtaccac ttgttggagc
     7321 ttgccatgcg acaccttcgt aatgccatcc ataacgtttc agattgtctc tttctccaac
     7381 gcttctagtg tatagatgct ctccgctatt gggattatac atacgataca tggaaataga
     7441 gctttctgct gaaactgaag gtgataataa tactagcccc attgtgaata gaattcctac
     7501 taaaaatttt ttcatttgat tttacccctt ttcatttttt attgtcatta ttgcatgaaa
     7561 attattcgta agattgccaa ttacataaac gattgtttta agatcataat cgttgttg
//
LOCUS       DANJMI010000093         7600 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000093 DANJMI010000000
VERSION     DANJMI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.096"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(943..1356)
                     /locus_tag="PWU81_002848"
     CDS             complement(943..1356)
                     /locus_tag="PWU81_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDL1111472.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="PWU81_002849"
     CDS             complement(1812..2297)
                     /locus_tag="PWU81_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111473.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="PWU81_002850"
     CDS             complement(2436..2651)
                     /locus_tag="PWU81_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDL1111474.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="PWU81_002851"
     CDS             2875..3675
                     /locus_tag="PWU81_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1111475.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="PWU81_002852"
     CDS             3694..5562
                     /locus_tag="PWU81_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL1111476.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="PWU81_002853"
     CDS             5555..6046
                     /locus_tag="PWU81_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL1111477.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="PWU81_002854"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="PWU81_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL1111478.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="PWU81_002855"
     CDS             6606..>7600
                     /locus_tag="PWU81_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL1111479.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DANJMI010000094         7141 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000094 DANJMI010000000
VERSION     DANJMI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.097"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..615
                     /locus_tag="PWU81_002856"
     CDS             <1..615
                     /locus_tag="PWU81_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL1111480.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            612..1385
                     /locus_tag="PWU81_002857"
     CDS             612..1385
                     /locus_tag="PWU81_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL1111481.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1382..1780
                     /locus_tag="PWU81_002858"
     CDS             1382..1780
                     /locus_tag="PWU81_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDL1111482.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1965..2636
                     /locus_tag="PWU81_002859"
     CDS             1965..2636
                     /locus_tag="PWU81_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111483.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2695..3135
                     /locus_tag="PWU81_002860"
     CDS             2695..3135
                     /locus_tag="PWU81_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111484.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3147..3815
                     /locus_tag="PWU81_002861"
     CDS             3147..3815
                     /locus_tag="PWU81_002861"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL1111485.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3879..4502
                     /locus_tag="PWU81_002862"
     CDS             3879..4502
                     /locus_tag="PWU81_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDL1111486.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4558..4944
                     /locus_tag="PWU81_002863"
     CDS             4558..4944
                     /locus_tag="PWU81_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HDL1111487.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5157..6548
                     /locus_tag="PWU81_002864"
     CDS             5157..6548
                     /locus_tag="PWU81_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL1111488.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         2277 a         1160 c         1572 g         2132 t
ORIGIN      
        1 tttgtttttg caccgttaga aggagaagac ctcgcttcat ttctggttgg tgcggcaatc
       61 atgatgatcg gaatgacgct ctttttgttt ggagcagatt attctatgat ggaagtcgga
      121 gatctagtag ggaaatatat gatcaaaaag aaaagtttga ccgttttggt cagtttggga
      181 tttgctatcg ggatcgtgat cacgattgct gaaccttccg tgcaagtttt gggacaacaa
      241 gtatatgata tatctgacgg agagattggc agagtgttgc tgattgggat cgtcagcgtc
      301 ggcactggag tatttctagc ctttgcgctg cttcgggtcg tattcaagct atcctattat
      361 caattgatgg ctataggcta cataggcgtt ttgatcgcct catttttcac cagttccgaa
      421 tttatgccga ttgcttttga ttcgggggga gtgactactg gtccagtaac tgttccattt
      481 atcttggccc ttgccggagg gatgactagt atgatcaaac aaaagaaaaa tgaaaatgac
      541 agttttggaa tggtcggtat tgcttcgtta gggccgattc ttgccgtgat gattttgggg
      601 gtgatcttcc aatgagtttc tggcagcaag tgatagatgg aatcgaaatc gttaccatgg
      661 aagtcatttt ggcagtggca cctatcgtaa tcctatttgt tttaatgaat atcattagtt
      721 ttcgtatgcg caaaaagcgt ttctttgaca ttatgaaagg tttcttgata acgactattg
      781 ggttgatcct tttcctgcac ggagtcaata tggcttatgt accggtagga cagcatttag
      841 gcagtgctat tgcgggattg ccttacaatt atatactggt tccccttggc tttttaatgg
      901 ggttcttagt agggtttgct gagccggcga ttcatgtaat gatcaaacag gttgaagaat
      961 tgagcgaggg gagaatccga tcgaaaatca tgctgatggt aatttcaatt gggatcggcg
     1021 cagcagtagg attgtctatg tggcgtttgc tcgtcggatt ctctctttat tatttcttga
     1081 tcccaggata cgggctagca tttattttag gacgaaaagt ggacaagatc tttttagcaa
     1141 tggcttttga taacggtgga gtagcaacgg gcccgatgtg ttcgacattt attttatcaa
     1201 tgagcgtggc aatcgctgca caaattgaag gaagagatcc gatcattgac gggtttggtg
     1261 tggttgcaat gattgcattg acaccaatat tatccacttt agtactagga tatatttata
     1321 aacgaaaaga tgaaaaagac agaatcgaaa aagaaagatt gcaaaaattg ggggaaagca
     1381 aatgaaagat cttgtagcat tgaatcttca catgattgtg acgatcgttg acggaggaat
     1441 gggcgaagat gttatcgact tttcaaaaga agcaggtgca gcaggcggaa cgatcttaca
     1501 tggaagaggt tctggtgtac atgattcagg gaaattttta ggtttggaca tttcgccaga
     1561 aaaagatatt gtgttgacat tagtacccga tagtttgacg aataaagtaa tggatgcgat
     1621 tggtcaaggc atcaacatcg atattccagg taatggtatt tgttttagta ttgatgtaga
     1681 caaagtcatt ggtatcacta agattcatca atatcgagaa attatcggaa tgcaagaact
     1741 tttagatgaa aataacgata gagaaaagtc tgaatcatag acaagcgttt gaagaggaaa
     1801 taaacacaaa caagaaggcg gatgagcgtt atttccgctt ttttgtttgt atttttctcg
     1861 tgaatactta tgaaatagta aatttttatg aaattctact ctaagcatca gactttcttt
     1921 tcaaaatatg ctattttaaa aatgatgaaa aaagggggag aaaaatgaat tttttagttg
     1981 ttttaaaaga tactctgatt gagcgattta catttctata tgttcaattg caggaaaata
     2041 aaacattgct gcatttttca ccagagttcc atctagaggg gctaacatta ggagaaatct
     2101 atcaagcaga agggctgtcg gctgttttgc acttttttaa ggtagaattg gaattaccag
     2161 taaaagatta tatcgagctt tctcttgaat cttgggatcg agcagtggtt gaactgtttc
     2221 cagaaggttt aatgatcgat acaaacgatg ggaagatcca tctgactgca gcagaactgc
     2281 aaaaacaaat gcgttatcaa atagatgacg aaaattcttt ttctattttt agacgtcagc
     2341 aaaagattct aaaaagcttt ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga
     2401 cacagctgtt gaaaaaatat ccaggtctgc ttcacacgtc tattcaattt cctcaattga
     2461 ttacgatgat ccatcgtttt atacgtatgc aacagctacc atctaagaag ctgtttcttc
     2521 cattgacgga cacatttcga gtagtcaaca gagaagataa aaagaaagtg attgtgattg
     2581 actttaccag aaatcggaat gtccttcacc aagttgcaat ggaaaaggcc aattaaggct
     2641 ttttcttttt ggattttttt gtcataataa atattagatg atggaggaga taatatgatg
     2701 caaacatggt tagaaaggct aatacgattt cagcagaact atcacaaact tatgcaggga
     2761 agatatgcga aatttgatca gttgaatcgc ggtctattga tcgctgcgct tgtctttagt
     2821 ttgctaagtc gttggttgcc ctatagaatc ggacaattgt tgttcatcgt ctttttcgga
     2881 tggatgatat ttcgtttcct atcaaaaaaa atctacccca gactaaatga gaatcaacgt
     2941 tatttgaaat atcttgatag agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa
     3001 gtcaaaatgg atcgaaaaag ttatactttt tttgagtgcc cgaattgtca tcaaaagcag
     3061 cgtgcaccta aaggaaaagg acgaatacgt gtgacatgca aaacatgtgg tataaaattc
     3121 gaaacgaatg tttaagggag aaaagaatga aattagtatt attacgtcat ggcgaaagcg
     3181 aagcaaattt tgaaaattac tggacaggat ggctagatgt cgccttaaca gaaaaaggaa
     3241 aagaacaagc agcagttgcc ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg
     3301 tttatacctc tgttttgaaa cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg
     3361 gtcagttatg gcttcctata ataaaaacat ggcgtctaaa tgaacggcat tatggggatc
     3421 ttgtgggtaa aaacaaagat aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat
     3481 ggcgcagagg atatacggaa gttccgccgt taaccgcaga aaatagattt gaccgcagat
     3541 atgatcactt ggatccgcat ttgatcccat acggagaaag tctggaaatg acagtgaatc
     3601 ggatcattcc cttatggcaa gatcatctag caccaaaact aaaagatcgg caagatttac
     3661 tagttgtggg tcatggtaat agcatccgag cactgacaaa atatttagaa gatgttcctg
     3721 aagatcaaat ggatacgatc gatatcccaa atgcacaacc aatccagtat acattagcag
     3781 atgatttgac aattctggac aaaaaaatac tttaaagaac acctttatct aaaagtgaga
     3841 gatagaaaaa agaagcaaag gaatggaaca aaaggaacat gaaaaattat atatgggact
     3901 ttgatggtac attgtttgat acgtatcccg caatggtaga tggtgcatgg caagcactaa
     3961 aagattttgg tatcagtatg gacaaaaaag aaatctactt caaaatgaaa aagtattcta
     4021 ctagttattt gatcaatgaa tcaaacttga atgccagaga gtttaatgaa ttatttcacc
     4081 gatatgaaaa agaaagtacg gaagtttccc gtccatttcc agaaacgaaa caagtactgg
     4141 aaatgctaaa agacaacggc ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa
     4201 catggggcct gttaaaagaa catcgattgg cacatctgat tgaggaagta gtagggatcg
     4261 atcaggactt tcctcgtaaa cctgatccag catctttaaa ttatttgatc gatacttttc
     4321 atttagaaag aacagacacg atgatgatcg gcgatcgtcg tttagatatc gaagcaggga
     4381 aaaatgcagg agtcgctact tgtctttacg acatcgatca ttttctgggt gagatcccgg
     4441 cagattatgt ggtaggaaat ctgaatgaaa tccttacctt gcagagcatc attaaaaagt
     4501 agtatcccct ttcaaaggag atatgttata attttcataa aggagtgata aaattatatg
     4561 gaaatcagaa gacaaaaaga agttgtagaa gcatttcatt atgatatgag attgccgaac
     4621 caagaagtag aaacagattt gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac
     4681 tatccgaaag aacatagcat cgtaggagca cgcttagagt tccgtttagt gtttgataca
     4741 tatgttttat ctggaagtgt gagtcagatc aatcatatcc tcaatcggaa aattgaaggt
     4801 caaaatgatg tcacacaaga agaagtggac gagctagtag caccgttatt cgatatcgta
     4861 cgccgaatgt cttatgaggt gacagagatc gccttagatc agcctggggt aaaattgaat
     4921 tttcaatcag aaaaacaaga atagacagta aaaacatacc tgcgatagat taaagctatc
     4981 acaggtatgt ttttttatta ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg
     5041 tctaaaatat aaaagtcact gaaaaaaatt ttttcaggag gatggttgaa gaatcattct
     5101 cgggaacctt tacttccttc gatggacttg tcattaataa tatgaggagg agaaaaatga
     5161 caacactcat cacagtttta gtggttctag tttttgcggc attattatat gtgttctatc
     5221 gtatgcaaaa gcgccataca aaattttcaa cacgcgtctt tacagcatta ggagttggta
     5281 tcgtctttgg cggtctgctt caattgatct ttggtgtaga cgacaaagtc gttactcaat
     5341 caatggaatg gatcgggatt gtaggtcaag gatatgtatc attcttgcaa atgctagtta
     5401 tgcctttagt gtttatttcg atcattggtg cattcacaaa aatgaaagtt tctgaaaagc
     5461 tagggaaaat tagtgcgact gtattgactt ctttacttgg aacaacagct attgcagcat
     5521 tgatcggaat tggttcaaca atgctgttcg gactccaagg agctaaattc acacaaggag
     5581 ctgcagaaac agcacgaatc tctgaactgg caacacgtca ggaaacagtt gaaaacttat
     5641 cgattccaca gcagatttta tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta
     5701 gtcgtgcaac aagtacgatt ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc
     5761 taggcgtgaa aagaaaagcg ccaaaagaag cagcattctt ttcagattta gtcgacagtc
     5821 tgtataaaat cattatgcgg atcgtgacgc ttgttttgcg tttgacacct tatggagtat
     5881 ttgctttgat gacaaacgtt ttggcaacaa gtgattttgc agctatcgtc aatttaggta
     5941 aatttgtcgt tgcttcttac gtagcattgc ttgctgtttt gattgtccac tcactgattt
     6001 tgattggtgc aaaagtcaat ccaatcaact atttcaaaaa agcctttccg gtattgagct
     6061 ttgcgtttac ttcacgttca agtgctggag ctttgccatt gaatatcgag actcagacaa
     6121 aagctttagg agtagatgat gcaacagcta atttctcagc aagttttggt ttgtctattg
     6181 gacaaaacgg ctgtgctggc gtttatccag ctatgttggc aacgatcgtc gcaccagcag
     6241 ctggagtgaa tgtctttagt ttggaattta ttttgatgct tgtggcaatt gtaacaatca
     6301 gttcttttgg tgtagctgga gtcggtgggg gcgcaacatt tgcttcactg atcgttttag
     6361 gggcaatgaa cttgccagta gcaatcgttg ggttagttat ctctgttgaa ccattaatcg
     6421 atatggcacg tacagcagta aacgtcagcg gtagcatggt agctggtatc gtgacaagtg
     6481 cacgcatcaa agatttagat cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga
     6541 atgcatagtc aaaaagaggt tgtgatagaa gtggtcagct ttaagaaata agaagaagtt
     6601 tcctaaaaat agtttctcaa atttttgtga aatttcggct tatttccgag aagctacttc
     6661 tgaaacaccg tgaataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
     6721 caaacaaaaa tagttcctca tattttacgt ttggttgagc ttattaccgc agatgcaagc
     6781 ttttgaacac cgtttaagaa agaggtcgtg agcctgccgt ttcactctga gtcacaaaga
     6841 gcaatttcaa aaaatagctt ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt
     6901 gtcaggagaa caccgtttac cagaggttgt gacttgtttt tagtcacgac ctcttttttt
     6961 atttattcca tataaataca cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa
     7021 caaccaactt atttattatt aaagaaaagc gtatttaagt aggacgattc tgttgagagc
     7081 tttcgtagat tttcccattt tcctatggta aactgacaaa gttgagaatg ttagaacaga
     7141 t
//
LOCUS       DANJMI010000095         6890 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000095 DANJMI010000000
VERSION     DANJMI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.098"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(<1..182)
                     /locus_tag="PWU81_002865"
     CDS             complement(<1..182)
                     /locus_tag="PWU81_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL1111489.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="PWU81_002866"
     CDS             517..951
                     /locus_tag="PWU81_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL1111490.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="PWU81_002867"
     CDS             974..1294
                     /locus_tag="PWU81_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111491.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="PWU81_002868"
     CDS             1435..1626
                     /locus_tag="PWU81_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111492.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="PWU81_002869"
     CDS             complement(1697..1954)
                     /locus_tag="PWU81_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDL1111493.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="PWU81_002870"
     CDS             2112..2375
                     /locus_tag="PWU81_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111494.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="PWU81_002871"
     CDS             2399..2587
                     /locus_tag="PWU81_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111495.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="PWU81_002872"
     CDS             complement(2670..2855)
                     /locus_tag="PWU81_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111496.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="PWU81_002873"
     CDS             3070..3441
                     /locus_tag="PWU81_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL1111497.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="PWU81_002874"
     tRNA            complement(3591..3663)
                     /locus_tag="PWU81_002874"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="PWU81_002875"
     CDS             complement(3709..4137)
                     /locus_tag="PWU81_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111498.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="PWU81_002876"
     CDS             complement(4197..4955)
                     /locus_tag="PWU81_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL1111499.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="PWU81_002877"
     CDS             5250..5447
                     /locus_tag="PWU81_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111500.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="PWU81_002878"
     CDS             5553..6035
                     /locus_tag="PWU81_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111501.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="PWU81_002879"
     CDS             complement(6093..6689)
                     /locus_tag="PWU81_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL1111502.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         2331 a         1059 c         1354 g         2146 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc ctcagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga atttttgctt
//
LOCUS       DANJMI010000096         6644 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000096 DANJMI010000000
VERSION     DANJMI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.099"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(1..859)
                     /locus_tag="PWU81_002880"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="PWU81_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="PWU81_002881"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="PWU81_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDL1111503.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="PWU81_002882"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="PWU81_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2603..3421)
                     /locus_tag="PWU81_002883"
     CDS             complement(2603..3421)
                     /locus_tag="PWU81_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL1111504.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="PWU81_002884"
     CDS             3582..4271
                     /locus_tag="PWU81_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL1111505.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="PWU81_002885"
     CDS             4285..5787
                     /locus_tag="PWU81_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL1111506.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="PWU81_002886"
     CDS             5800..6276
                     /locus_tag="PWU81_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL1111507.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="PWU81_002887"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="PWU81_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DANJMI010000097         6532 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000097 DANJMI010000000
VERSION     DANJMI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.100"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..942
                     /locus_tag="PWU81_002888"
     CDS             <1..942
                     /locus_tag="PWU81_002888"
                     /inference="COORDINATES: protein motif:HMM:NF016640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF616 domain-containing protein"
                     /protein_id="HDL1111508.1"
                     /translation="FMGRQELYSLAINELEKRNLEIHELNQKNNSILGMKIRNKINQL
                     NQMKKRDNLLPAKYTLKSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIAVYSCI
                     TGNYDSIKEPDFSHPLVDYYLYTDNVITSEHWNIREIPKYISELKNNILINRYIKFHP
                     NELFTDYDFTLYVDGNVTITSDITGLLNSSSKLGLSMHKHSERKSLYSEADALIAVGK
                     GNKESIIEQISKYREEGFPDNFGLAEATVILVDLNNSIAENLLDLWWREYLNSESGRD
                     QLAFPYVLWKSGHAMKEVTTLGNNVYKSAKLRIISHY"
     gene            1188..2204
                     /locus_tag="PWU81_002889"
     CDS             1188..2204
                     /locus_tag="PWU81_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HDL1111509.1"
                     /translation="MKMKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQL
                     KEDRLTILYDYNNFKFIRMDLLEKEKLDSLFESEGFDIVIHLAAQAGVRYSIENPYAY
                     IDSNIIGFLNILEACRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKK
                     SNELMAHTYSHLYKIPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKME
                     RDFTYIDDIVEGITRLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFV
                     ETIEKYLGVKANKIFLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTV
                     YYLS"
     gene            2572..3996
                     /locus_tag="PWU81_002890"
     CDS             2572..3996
                     /locus_tag="PWU81_002890"
                     /inference="COORDINATES: protein
                     motif:HMM:NF014051.1,HMM:NF026018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HDL1111510.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFPNLEYYQLFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLL
                     LLTIFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYS
                     IPLTLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKFSSIILTLTSIVSMSMIEDA
                     VITSENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAYSFITNPDYKKSLVLVPILV
                     IMACFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLLGYSFGVIGVSFATLL
                     GTLVLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHRGFYFNLFLTIIFSAI
                     FVFTYKQLIITYLKKFFRIKMAKR"
     gene            4226..4510
                     /locus_tag="PWU81_002891"
     CDS             4226..4510
                     /locus_tag="PWU81_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111511.1"
                     /translation="MSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDNVIIRANT
                     LVNKDIPNNSVVAGSSCKVISTIEEHYEKNKNEFIKYRTSMILKQKNFFY"
     gene            4598..5764
                     /locus_tag="PWU81_002892"
     CDS             4598..5764
                     /locus_tag="PWU81_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HDL1111512.1"
                     /translation="MKISVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISP
                     IEDKEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIE
                     KALAIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIV
                     GECSARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYA
                     EFRGLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAI
                     VDANTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVV
                     YEPTLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDIFGDN"
BASE COUNT         2261 a          860 c         1210 g         2201 t
ORIGIN      
        1 tttatgggaa gacaagaatt atatagttta gctatcaatg aattggaaaa aagaaatctt
       61 gaaatacatg aattaaatca aaaaaataat tctattttag gcatgaaaat acgaaataaa
      121 attaatcaac ttaaccaaat gaaaaaacga gataatctgt tgccagcaaa atataccctt
      181 aaatcgatga ccggtaaggg gcctattaaa gactattcaa acagagagaa tcgcagtgat
      241 gaactaagaa aaaaagagtt aactaatcaa ggaacaccta aaaggattgc tgtgtatagt
      301 tgtatcactg gtaattatga ttctattaaa gaaccggatt tttctcatcc attggtagat
      361 tactatttgt atacagataa tgtgattaca agtgagcatt ggaatattag agagatacct
      421 aaatatatat cggaattaaa aaataatatt ttgatcaata gatatattaa atttcatcct
      481 aatgagctat ttactgacta cgattttact ttgtatgttg atggtaatgt aacaataact
      541 tctgatatta ccggattact gaacagttct tccaaacttg ggttaagtat gcataaacat
      601 agtgaaagaa aatctttata ttctgaggca gatgcattaa ttgcagttgg caaaggtaat
      661 aaggaaagta ttatagagca aattagtaag tacagagaag aaggatttcc tgataatttt
      721 ggattggctg aagccacggt tattttagtt gatttaaata attcaatcgc tgagaatctt
      781 ctagatttgt ggtggagaga gtatttgaac tcagaaagtg ggcgagatca gctcgcattt
      841 ccttacgtgt tgtggaagtc cggacatgca atgaaggaag tgactacctt aggcaataat
      901 gtatataaaa gcgctaaatt aagaatcata agtcattatt agccctaaat gatttctaaa
      961 ctattctttc aaatcctagt aaatatttgg agatagaaga tttccttttt aaagtcataa
     1021 aaaattaagt tatatttttt gagtgaatgg agaagacgca tagtatcata aacttcgtta
     1081 tatcttaaat tatggggtat gcctaaataa taaatgtaat acgagtataa agaagaaagc
     1141 aattttgggt ttaaccctat tggttccttt cagaaaaagg tggatgaata aaaatgaaaa
     1201 tattaataac aggtggagca ggatttattg gttttcattt gtctagaaaa ttactcaagg
     1261 aaactgaaaa tcaaattgta gcgattgata atctcaatga ttattatgat gtccaattaa
     1321 aagaagatcg attaacgata ctgtacgatt ataataattt taaatttatc agaatggatc
     1381 tattagagaa agaaaaatta gattctctat ttgaaagtga aggttttgac atagtaattc
     1441 atttagctgc tcaagcaggt gttagatatt ctattgaaaa cccttatgct tatattgatt
     1501 ctaatattat cggttttctt aatattcttg aagcctgccg aaacttccct gtaaagcatc
     1561 tattatatgc atcatctagt tcagtatatg gtgggaacac gaaagtaccg ttttcaacag
     1621 aagattcggt ggatcatcca gttagccttt atgcggctac caaaaaatcg aatgaattaa
     1681 tggctcatac ttatagccac ctttataaaa taccaacaac tgggctgaga tttttcactg
     1741 tctatggtcc atttgggcga ccagatatgg cctatttctc ttttaccaag aatattttat
     1801 ctggaaaaga aattaagatt tttaaccatg gaaagatgga aagagatttc acttacatcg
     1861 atgatatcgt agagggaatc actagattaa tcaataaagt acctgaaccc aactccgact
     1921 ggacagacat cgatgatttg agtagtgaaa gttgggcacc atataagata tacaatattg
     1981 gaaataataa gccggttgca ttgttggagt ttgttgaaac aattgaaaag tatttaggtg
     2041 ttaaagcgaa caagattttt ttggacatgc agccgggtga tgtcgttaga acttatgccg
     2101 atattgaaga tttagaggaa aaaatcaact ttaaaccgaa tataaccttg gatgagggca
     2161 ttaaaaaatt cgttgaatgg tatacagtgt attatttgag ttaattttga aaggaatatt
     2221 gaagatgggt aattcatacg atttttttag ctttgttagg tattttagga aattctactt
     2281 tcaaacgtga cgaactttct cgccgaatag atattttcta taactaaaag aatttctata
     2341 aaacaaaatt gtgtatcaag atgtgtgaac tggattctga ctgccaatat tgattgatga
     2401 gattgaagaa atatatagtt gatcatggat atctttattt gaaaagatct tagctattca
     2461 catattcaaa aaaattttta ctacgaggac tcacacgata cagaggaatt taggtagttc
     2521 taattaaacc agaaagtcat ctatagatgt gagttgaggg aggtcatacg gttggatagt
     2581 agcagtaata aatcacattc aatagctaaa aaagctttta tttacgcaat aggaacatta
     2641 tcatcaaaaa tgatatctgt cattataatc cctatctatg cagtatatat ttctgttgcc
     2701 gatttaggaa gctttgatta tcaaaataca attgctcaat ttctttctcc agttattttt
     2761 ggagcaatat gggaagcaat tttacgcatg ggattaggca aagaaaaaaa tattcagaaa
     2821 aaagtagtga cgaacagtat ttgttttgtt atagcaaata cagtgatatt agccattttt
     2881 ttaccaataa tatatagaat gattttcccg aatttagagt attatcaatt gtttgttttt
     2941 caattaattt tcactcctat cctaagtgtt tttcaatata ttgtaagagc actaggtgaa
     3001 tcaaagattt ttgtgatgag tggtataatc gctgcgggta tcaacctgtt actccttact
     3061 atttttgttg ttacaatggg tatggggata cttggtctct tactctctat cataatttct
     3121 aatgttataa atttgatttt tttgattttc agggggcaga tctttcaata tttcgcaacg
     3181 agttcttttg attttttatt tttaaagtcc atgctgaaat actctatccc actcactttg
     3241 aatctcactt ttggatggct aatagttggt ttttcaagat tttatattgg aacgtacatc
     3301 ggagtaacag aaaatggtat tttttctttt gcgatgaaat tttcatctat aattttaact
     3361 cttacaagta ttgttagtat gtctatgata gaggatgcgg ttatcacgtc tgagaatgat
     3421 gatttcatcg aaagattcgt tgagaaaaat acctatattt ttaaaatgta cattaggtta
     3481 gcgactcttt ctattcctat catttgctgt gcatactctt ttataaccaa ccctgattat
     3541 aaaaaatcat tagttctagt tccaatatta gttattatgg catgtttcag tgggatgaca
     3601 acaaatatcg gaaatatatt tgcagtatac aataaaacaa atcagttatt aatgatttca
     3661 gtagtgtcag gtctattgag tgttgttggc acctatttat taggctattc ttttggagta
     3721 attggtgtct catttgctac tttgctcggt actttagtat tatttggact tagatattat
     3781 tttggaagga aaattgctta ttatgcattt gattggaagc gaatatcgct acttttagtt
     3841 ctctattccg caatcgcttt tgtatgcatt catagaggct tctattttaa tcttttctta
     3901 acgattattt ttagtgctat tttcgttttt acgtataaac aattaattat aacgtatcta
     3961 aaaaaatttt tcagaataaa aatggctaaa aggtgagggt atacgaagta agaatagaat
     4021 ctttcgaaga ataaagagaa aactatttgg aaaaaatctg cgagaatgta ttaatgaagg
     4081 tttgatattc ggaaaaaaac atagtttagt agaggttcct gaatttggga gcgagccata
     4141 tttaattgaa ataggggata atttcagatg ttcatttggt gttaaattta taacacacga
     4201 tggtggagtc tgggtcttaa ggaagatgag ttctgtgagc gagtcaactt caaaaatagg
     4261 taggatcaaa ttgggaaata atatttttat tggtatttac tctattgttt tcccaggagt
     4321 ttcaattggc gataatgtaa ttattagagc gaacacttta gtaaataaag atatcccaaa
     4381 taatagtgtt gtagcgggta gttcatgtaa agtgatttca actatagagg agcattatga
     4441 gaaaaataaa aatgaattta ttaaatatcg aacctcgatg attttgaagc aaaaaaattt
     4501 tttctattaa ataatattga ttgaataagg aatatgttca aaaggataac agctaaaata
     4561 ctataaaatg aaatttgatt ggagcgtatg gaagaatatg aaaatttcag ttttcggatt
     4621 aggctatgtt ggtctagcaa attcactatt attagcacaa aaagagcagg tcaaagctta
     4681 tgatatcgtg gaagaaaaaa tcacgatgtt acaaaatggc atctcaccaa tagaagataa
     4741 agaagttcat gaatttttaa aacgggatga cttaaatgtc gaatttacgt caaattttac
     4801 tgatgcagta aacttcggtg attacttgat tattgcaact ccaacagatt atgacgaaaa
     4861 gaaaaactat tttaatacat caacagtaga agatgtaatt gagaaggctt tagcaattcg
     4921 tccagattca acattcatta ttaagtcgac agttccagtt ggctatgcaa atgaaattaa
     4981 agcgaaattt ggtacagaaa acatcatttt ttcacctgaa tttttgcgtg aaggaaaagc
     5041 tttgtacgac aacttatatc catcaagaat tatagttgga gaatgttctg ctcgtggtcg
     5101 cgagattgca actatgtttc aaagaaacgc atttaacaaa gatgcatcag ttcttttgac
     5161 tcactcaact gaagcagaag ctattaaatt attctcgaat acatatttgg cattgcgagt
     5221 ggcgtatttc aatgaattag atacgtatgc agaatttcgt gggcttgata gtaagcaaat
     5281 tattgaaggt gtcggtttgg atccacggat cggttcgcat tataataatc cttcttttgg
     5341 ctatggtgga tactgtttac ccaaagacac aaaacaatta agagccaact atgaaaaagt
     5401 accaagcaat atcatcagtg caattgttga tgcaaataca acaaggaaag attttattgc
     5461 tggacagatt ttggcgaaaa aaccaaatgt tgttggaatc tatcgtttaa cgatgaaggc
     5521 cggttcagat aattttcgat attcgtcgat ccaaggaatt atgaaacgga ttaaagccaa
     5581 aggaattgaa gtggttgttt acgagcctac attggatgaa gatgaatttt atcattcaca
     5641 agtattgaaa gatattaatg aatttaaaaa tatttctgat gtgatcgtat ctaatcggtt
     5701 tgaagatatt ttaaaggatg ttgaagaaaa agtttatacc agagatatct ttggtgataa
     5761 ctaaataata atgaacgggt acatggagga aaatatttta gatggtgact cgaaataaat
     5821 gtttgttctc attgcatctg tatctatacc gataagttta aaaattccga ttaggaaaat
     5881 gaagttggct atccttgcga ggaatttatt gctggattat tttatgaact aaagcaagac
     5941 atcatcagaa aaaaatccaa aatttgacaa tgggattgat cgtttaccag ctgtaagcga
     6001 agtatctgac acgaaaatgg aaaatgcaat tactgttatt accaatagct ttttttcatc
     6061 gatcaagtat ttgaaaacat tatttgtttg ccaagctgat caatgttctt tcagacttgg
     6121 aagaacattg atcagctcac aagtaatgat tgaagtttag gttatgggat aaaggataag
     6181 tgaaataaaa gtcgtgtctg catctttgtt agtaccagta aatttttcta ctacggcgct
     6241 agtcacgaca gtccaggcat ctgaaattga agatgcaaat agagtttctg ctagtttagg
     6301 tattgatgtt actgtattag cattaatctt agagccagta gcatccaata ggtgtagcta
     6361 tagttctaat agtataaaac aatgcagagg atatgattgc tttatgtctc ggtgtgtcag
     6421 gtcaaaatag agttccatat ggggcgattt ctcaatgaat aatcttatag atataatctt
     6481 gtttgctatt tcgttatcac ttttactttt cttcgattat ttcaaaaata aa
//
LOCUS       DANJMI010000098         6352 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000098 DANJMI010000000
VERSION     DANJMI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.101"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(99..1229)
                     /locus_tag="PWU81_002893"
                     /pseudo
     CDS             complement(99..1229)
                     /locus_tag="PWU81_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="PWU81_002894"
     CDS             complement(1878..3080)
                     /locus_tag="PWU81_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1111513.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="PWU81_002895"
     CDS             complement(3140..3343)
                     /locus_tag="PWU81_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HDL1111514.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="PWU81_002896"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="PWU81_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="PWU81_002897"
     CDS             4880..5692
                     /locus_tag="PWU81_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1111515.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1898 a         1301 c         1002 g         2151 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagctaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac ag
//
LOCUS       DANJMI010000099         6337 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000099 DANJMI010000000
VERSION     DANJMI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.102"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            290..1135
                     /locus_tag="PWU81_002898"
     CDS             290..1135
                     /locus_tag="PWU81_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1111516.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="PWU81_002899"
     CDS             1416..2636
                     /locus_tag="PWU81_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HDL1111517.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="PWU81_002900"
     CDS             2633..5353
                     /locus_tag="PWU81_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1111518.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="PWU81_002901"
     CDS             5337..6281
                     /locus_tag="PWU81_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL1111519.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2363 a          982 c         1223 g         1769 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 tcgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgcttt
//
LOCUS       DANJMI010000100         6303 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000100 DANJMI010000000
VERSION     DANJMI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.103"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            334..714
                     /locus_tag="PWU81_002902"
     CDS             334..714
                     /locus_tag="PWU81_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDL1111520.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="PWU81_002903"
     CDS             696..1610
                     /locus_tag="PWU81_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL1111521.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="PWU81_002904"
     CDS             1855..2544
                     /locus_tag="PWU81_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111522.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="PWU81_002905"
     CDS             complement(2665..2751)
                     /locus_tag="PWU81_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL1111523.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="PWU81_002906"
     CDS             3427..4377
                     /locus_tag="PWU81_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDL1111524.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="PWU81_002907"
     CDS             4398..4931
                     /locus_tag="PWU81_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL1111525.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="PWU81_002908"
     CDS             4947..5117
                     /locus_tag="PWU81_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111526.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="PWU81_002909"
     CDS             5567..6100
                     /locus_tag="PWU81_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111527.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DANJMI010000101         6224 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000101 DANJMI010000000
VERSION     DANJMI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.104"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(207..1169)
                     /locus_tag="PWU81_002910"
     CDS             complement(207..1169)
                     /locus_tag="PWU81_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL1111528.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="PWU81_002911"
     CDS             complement(1171..2610)
                     /locus_tag="PWU81_002911"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDL1111529.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="PWU81_002912"
     CDS             2795..4741
                     /locus_tag="PWU81_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HDL1111530.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /locus_tag="PWU81_002913"
     CDS             5005..5877
                     /locus_tag="PWU81_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL1111531.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1986 a         1061 c         1107 g         2070 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaggc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa ccgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacaaaaa aaagggggtg cggatatttt tttggacctt tttaacaaat
     6121 aggttctgtt gcaaagtttt aaatctacta tcagataagg tagaataata gaaaaagata
     6181 gcaggaggaa tgacgatgag tcattttaaa ggaaagcaat ttca
//
LOCUS       DANJMI010000102         6172 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000102 DANJMI010000000
VERSION     DANJMI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.106"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            725..811
                     /locus_tag="PWU81_002914"
     CDS             725..811
                     /locus_tag="PWU81_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL1111532.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="PWU81_002915"
     CDS             complement(932..1621)
                     /locus_tag="PWU81_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111533.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="PWU81_002916"
     CDS             complement(1866..2780)
                     /locus_tag="PWU81_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL1111534.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="PWU81_002917"
     CDS             complement(2762..3142)
                     /locus_tag="PWU81_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDL1111535.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3591..3878)
                     /locus_tag="PWU81_002918"
     CDS             complement(3591..3878)
                     /locus_tag="PWU81_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL1111536.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3898..4122)
                     /locus_tag="PWU81_002919"
     CDS             complement(3898..4122)
                     /locus_tag="PWU81_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HDL1111537.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4577..4747)
                     /locus_tag="PWU81_002920"
     CDS             complement(4577..4747)
                     /locus_tag="PWU81_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111538.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4762..5295)
                     /locus_tag="PWU81_002921"
     CDS             complement(4762..5295)
                     /locus_tag="PWU81_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL1111539.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5316..6158)
                     /locus_tag="PWU81_002922"
     CDS             complement(5316..6158)
                     /locus_tag="PWU81_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL1111540.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1150 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggaag ctctatattt gctagccaaa tacctacgta tttggtgcaa ataaccgctt
     3301 gtcaaagggg cgcaagctcc cccttgaatc cccctccccc ccactctcca caatacaaaa
     3361 aagactagca aatgctagcc actttttgta aagtttcgct tgtttcacct tcggtgaaat
     3421 tgttgcaaat caataattag gtagagctac atttttttcg ttgttcaaag attcttatgc
     3481 catttactat taataaatct ataaatatga taatttttcg gctctataat ttatgggact
     3541 gatgaatcag aattgattct tcagtcccat tttcatttta ttccaaagaa ttaactccaa
     3601 ataccaatag aagcccatcc tctatatact tgagattcct tactaacaat ttcttgtaat
     3661 tctattaaag atttaggtaa tccaaattta tgttttaaaa tatatcttcc aagaaaacta
     3721 cttaatttca tactaacttc accaacactt tttccctcac tgatttcttt agaataaaga
     3781 aagagctttt cttttaagtc aggatagtca tctatagttg cttcattgta tactttacta
     3841 attgcattta aaagtttttc ttctttagta aaatccatta taataacctc cctaccacta
     3901 ccttctaggt gcccatggac catgattaac ccaaccatta acaataattt ttccaatttc
     3961 tcctttagct tgattccaat ctacccaaca tttttctttg ttacaataaa gaccatttcc
     4021 atagtaagta gctgcatcaa cttttattcc tgtaccgatg ccagctagac aagttcctaa
     4081 tattcctaga ataacacaat gttttaatac tttctttttc atgattgttt ttccttttcg
     4141 tttttttaaa attgcttgtt atattaaata atcttagtaa ttttattata taacatttta
     4201 ttatataaca atgattaacg tcataaaaag tagttttgaa ttcataatcg tttaaataaa
     4261 attataagat cagttattta aacagctagt agacataatg tgtatactac caagtaacgc
     4321 ctgtaccaag ggatccaggc gctgattttg tgaattttta ttggtctagg ttaaaagctc
     4381 tttttaaggc ttggttttct gcggttgttt ctttctaatt ctttgtgaat attgatcggt
     4441 cattttttaa atacttgtta tttttgtttg tgtttgttat gctttttaca tatctcatat
     4501 tcatttatta ttcttttcta aggttaataa taggataatc tctttcagaa agactgctcg
     4561 tacgagcagt cttttcttat ttatcaagtt tttctacaaa tacttcctca ttttgactgt
     4621 tgtagtaatt aggattgagt tttagttccc cttttttatt tcctttgttg gccactgata
     4681 ataacattgg gctaaagctt aatattccta gtaggataga accgaatagc aagtgttttt
     4741 ttgtcataag tatttccttc ttcatttcca tagctgccac cattttttcc aattctttgt
     4801 ttgttcgtcc attgtttctg ttgcttcaga aagagtcgtt gaactattct ctgaagactc
     4861 ttccgtataa gtcaactgca gttgctcctg aagtttttcg atttgttggt tggcttgcag
     4921 ggtgagttgt tgttgctggt cgagtagttt ttgtatatga gaattatttt gctctttgag
     4981 ggcaagttgt tctcgtaaaa attcattttc ttcacgcaac tgatctagca acttgtttgc
     5041 aaccacgttt ttgtttggta tataagcatt tttatcgttg ttttgttcat gtttatgcaa
     5101 cttatctagc aacctttgat tgtctaagtt gcgagcaagt tgcaatactc cagaacgcaa
     5161 tacaacctta taaccacgct catttttagc tatcatggaa gaatctaatt tttggatatg
     5221 tttcgatatt gcttgcctac tcatcttaaa aagatctgac aattccttta ttgtaaaggt
     5281 ttcttgtggt tgcatactgg ttgcctccta ggttgctact ggaataaatt atctaaatca
     5341 gataataaac tttttttctc tttttccgaa agtgcggact ctgtttgttt ttcaagagct
     5401 tttttctctt tttctagttg tttgatttgt tggaggagtt caagttctcg tttcttctgt
     5461 ttgatttttt cgttaattgt tgtagcgggt tcttcgtatt ctttttgttt atcagctaaa
     5521 gccaatgctt tattttggtc ttctttggtt ggtgcatgtt tgttaaagtc gtctgctttt
     5581 ttgatctcag gtttgaacgt aaattgatag ccaataacag gttttccccg tccttttccg
     5641 tatttctttt tgattgttag tcctttgaag attgcagtta gttcttgacg tataggtttt
     5701 ataactcgtt tatctatatc aggttgctta taacttttag gaatatctaa taattcaaga
     5761 aagtcttctt tggagaaata tgcgaaacct ttggttctgt attgctttaa cagacgaaac
     5821 atagtctttg catagccact ttgtagctcg ttgaattgtt gtaaagaata gcgtacccac
     5881 tcgtctaaat tatttaacaa aggcagtgct ttttcgtaaa ttcgtatatc tacgtaagga
     5941 ttttctacgt ccccgttgat tttaaattct gtaaacatta caaacatatc gtaagaaagc
     6001 ccagactcac ttctacgacc aaagcgtaaa gataagattt tttcataagt tgcttttaaa
     6061 tcttctacaa atcgtacatt tgcagttgct ttgtagttac ttaaatcttt tagttggtca
     6121 aacgtaaaac gaactgtctt atcccctttt tcacgcattc tagacacgat gg
//
LOCUS       DANJMI010000103         6086 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000103 DANJMI010000000
VERSION     DANJMI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.107"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(<1..2387)
                     /gene="esp"
                     /locus_tag="PWU81_002923"
     CDS             complement(<1..2387)
                     /gene="esp"
                     /locus_tag="PWU81_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HDL1111541.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVS"
     gene            complement(3004..3438)
                     /locus_tag="PWU81_002924"
     CDS             complement(3004..3438)
                     /locus_tag="PWU81_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111542.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3455..4660)
                     /locus_tag="PWU81_002925"
     CDS             complement(3455..4660)
                     /locus_tag="PWU81_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HDL1111543.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5354..5740
                     /locus_tag="PWU81_002926"
     CDS             5354..5740
                     /locus_tag="PWU81_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL1111544.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5834..6025
                     /locus_tag="PWU81_002927"
     CDS             5834..6025
                     /locus_tag="PWU81_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1111545.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT         1979 a         1083 c          837 g         2187 t
ORIGIN      
        1 aagcttacgc cgtctttcac tgcaaagact gttgttccac tttcaatact tgttccttcc
       61 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
      121 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      181 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      241 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      301 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      361 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      421 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      481 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      541 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      601 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      661 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      721 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      781 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      841 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      901 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      961 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
     1021 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1081 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1141 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1201 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1261 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1321 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1381 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1441 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1501 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1561 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1621 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1681 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1741 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1801 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1861 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1921 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1981 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     2041 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2101 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2161 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2221 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2281 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2341 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2401 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2461 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2521 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2581 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2641 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2701 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2761 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2821 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2881 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2941 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     3001 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3061 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3121 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3181 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3241 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3301 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3361 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3421 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3481 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3541 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3601 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3661 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3721 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3781 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3841 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3901 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3961 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     4021 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4081 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4141 aattttgtga taacagatta tctattttga tttgctcaat agttttactt agattttctg
     4201 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4261 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4321 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4381 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4441 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4501 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
     4561 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
     4621 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
     4681 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
     4741 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
     4801 agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata
     4861 tctagttcaa agacaataat tattatgatc cgttattatc taagcactat acagataata
     4921 ggaatttttc gtttctaaat attccaattt gggaatccaa taaaaatttt cgttcaaata
     4981 cgtgtctatt cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac
     5041 aatctataca cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg
     5101 taaaaagctc aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat
     5161 gaattttctt ttctaagtat aaagataact atccattatc aactaaaaaa acatttttga
     5221 aaataaacat cagcagtcct atcaatgcat gtgagcttat agaagttatt cgcagcgcag
     5281 cataattgca ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga
     5341 ggaggtgtgc aatatgtcag ataacgacga acgcgtaaat gcccagttga ttcgattctt
     5401 tgaaaaggtc attttgaaca ccgctaatac ttattataaa aaacaagaag aaattcaaaa
     5461 acatgaacaa cttgatcaac tcccaccata tttgtttact aaagatacta taaaatttaa
     5521 acaaccgatt aatgttttaa atgtgactct ccttgttgaa gatataaaat tagcaaaaat
     5581 ccttcctttg ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta tctttgataa
     5641 aactgataaa gaaattggaa aggttctggg gattacaaga cagggtgcga caaatttaaa
     5701 acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata ataactaatt
     5761 ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa tagattataa gtccatggga
     5821 agaaggtaga tttatgaatt ctttaaaaga agatttcatt ttagcaaagg ctggaaatga
     5881 agaagctgtt gaggcaattc tcaaaagatt tagctctttg attcataagc aatcctggag
     5941 aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat
     6001 ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata agtaaaaaga
     6061 tagtttcaaa tttcgaacaa taaatt
//
LOCUS       DANJMI010000104         6008 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000104 DANJMI010000000
VERSION     DANJMI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.108"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(<1..450)
                     /locus_tag="PWU81_002928"
     CDS             complement(<1..450)
                     /locus_tag="PWU81_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL1111546.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="PWU81_002929"
     CDS             674..1465
                     /locus_tag="PWU81_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL1111547.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="PWU81_002930"
     CDS             1431..2384
                     /locus_tag="PWU81_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HDL1111548.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="PWU81_002931"
     CDS             complement(2782..3756)
                     /locus_tag="PWU81_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL1111549.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="PWU81_002932"
     CDS             3871..4305
                     /locus_tag="PWU81_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL1111550.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5452)
                     /locus_tag="PWU81_002933"
     CDS             complement(4430..5452)
                     /locus_tag="PWU81_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL1111551.1"
                     /translation="MKKIGFLSFGHWMEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGA
                     FFRVHHFAPQIGSPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQG
                     RVQLGVGRGSPEQVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPM
                     FPQGSGKLRIEPYSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQ
                     QANQLRAYKEAWKQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYH
                     RNPTIFGKTFAGEPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIA
                     PELGWR"
     gene            5844..>6008
                     /locus_tag="PWU81_002934"
     CDS             5844..>6008
                     /locus_tag="PWU81_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HDL1111552.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIMAEDG"
BASE COUNT         1892 a         1133 c         1037 g         1946 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaaa ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga
     5941 taagaagtgc ggggatcgag acacatggct tgaatccaag agctataaaa atcatggcag
     6001 aagacggc
//
LOCUS       DANJMI010000105         5874 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000105 DANJMI010000000
VERSION     DANJMI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.109"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            196..921
                     /locus_tag="PWU81_002935"
     CDS             196..921
                     /locus_tag="PWU81_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111553.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGFSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="PWU81_002936"
     CDS             911..1420
                     /locus_tag="PWU81_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111554.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="PWU81_002937"
     CDS             complement(1490..2938)
                     /locus_tag="PWU81_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL1111555.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="PWU81_002938"
     CDS             complement(2938..3654)
                     /locus_tag="PWU81_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1111556.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="PWU81_002939"
     CDS             complement(3635..3985)
                     /locus_tag="PWU81_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL1111557.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="PWU81_002940"
     CDS             4129..4902
                     /locus_tag="PWU81_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL1111558.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="PWU81_002941"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="PWU81_002941"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(5744..>5874)
                     /locus_tag="PWU81_002942"
     CDS             complement(5744..>5874)
                     /locus_tag="PWU81_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL1111559.1"
                     /translation="QVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT         1706 a         1142 c         1023 g         2003 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tttttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt ttttttaatt attatttgtc aaattaagcg agacaaaact
     5761 ttccatttac gtaactcaaa aaaacttcta atggtgtttg gtaatttagt gattttctag
     5821 ggatatggtt tcttttggac gcaacggatg agatgaatcc ttgattgact tggt
//
LOCUS       DANJMI010000106         5481 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000106 DANJMI010000000
VERSION     DANJMI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.110"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(182..799)
                     /locus_tag="PWU81_002943"
     CDS             complement(182..799)
                     /locus_tag="PWU81_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111560.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            complement(861..1541)
                     /locus_tag="PWU81_002944"
                     /pseudo
     CDS             complement(861..1541)
                     /locus_tag="PWU81_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(1863..2084)
                     /locus_tag="PWU81_002945"
     CDS             complement(1863..2084)
                     /locus_tag="PWU81_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111561.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(2182..2427)
                     /locus_tag="PWU81_002946"
     CDS             complement(2182..2427)
                     /locus_tag="PWU81_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111562.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            2667..3281
                     /locus_tag="PWU81_002947"
                     /pseudo
     CDS             2667..3281
                     /locus_tag="PWU81_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(3651..4652)
                     /locus_tag="PWU81_002948"
     CDS             complement(3651..4652)
                     /locus_tag="PWU81_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HDL1111563.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(4811..5077)
                     /locus_tag="PWU81_002949"
     CDS             complement(4811..5077)
                     /locus_tag="PWU81_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1111564.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(5067..5423)
                     /locus_tag="PWU81_002950"
     CDS             complement(5067..5423)
                     /locus_tag="PWU81_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL1111565.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1696 a         1012 c          752 g         2021 t
ORIGIN      
        1 aaaacgtaga aaattacgtc tacgttttcc tatttagttc tatattttca ttaaatttta
       61 tatttgctct tttcactttt cggaataaat tcactgtgac tcttgatacc ttcttttata
      121 tattgctaat gtattttctc cattgctatg aaaatagaat caacatattt ataatatcta
      181 tctaatctat tttcatttca ccgcagcatc ttattaaata acgtgtaaat aagaatataa
      241 aagttattga actaattaat aaagatagaa tcatcgtagt tggatacgta gatgaaacct
      301 caactatagt tcttttcccc gtacctttaa tagacactaa accattgtca ttttctatag
      361 tgctcttttt cccatttact ttcacaactg ttccccaata attgaataca ggtaatgtga
      421 tattttgctc atcggcatct tctactgtat agactatttt ggaattatat gctttatgag
      481 atgcattctc ccaccgatca ccaactttta cttcatgttt ctttaaatga tctttatatt
      541 tcaatgcatc taatggtgca tagtcatata ggcctccagt tcttgaggat gtataacgtg
      601 taacatcttt tttggtcata actaaacgac ctttatacga ctgaatttta tgcgaataat
      661 ttgcttctgt tgctgcagtc acagtaaaaa taataagaac tattcctaca aggctccaca
      721 tacttttttt gttgcttgtt ttccacttta aaaacactat ggttaagatt aatgaaccaa
      781 aaagcacttg aaaacccaag gttctcggtt ctgttgcgaa gtttgaaaat caaacgcgcc
      841 ctctctgaac tccaaatatc ttaggctgtt attcccatta ataccttgat ttcagtagac
      901 accgaaaagc cgaagagcgt tccatttctt cggttctttt tatatattcc tcgaatggtc
      961 tccatgccct taatcgtgga agaggctgta cggagacttt gataaaattt atttcgtcgt
     1021 ttaataggtc gatggtcttg ttctattaaa ttgttaagat acttcacagt tcggtgctct
     1081 gtcttagtat ataaacccac actctgtaac tttctaaagg cggagccaag agaaggtgct
     1141 ttatcggtca caattgcttt cggctcacca aactgtttat ggagtcgttt taagaaagca
     1201 taggctgctt gcgtatcccg tttctttcgt aaccagatat ctaaggttaa gccgtccgca
     1261 tcaattgcac gataaagata atgccaacgt cccttaattt tgatataggt ttcgtccatt
     1321 ttccatgaat agaaggattg tctatttttc ttcttccaaa gataatagag gactttgctg
     1381 tactcttata cccaacgata aatcgtagta tgacaaacat ttattccacg atcatataac
     1441 aattcctgaa cttcacgata gcttagattg taacgcaggt agtaaccaac agcgacaata
     1501 atgacgtctt ttttgaattg tttgccttta aaatgattca ttactctgtc ctctctgtct
     1561 tttttctcaa ttttacacta aaatagattt tttggaaaac tttgcaacag aaccgttttt
     1621 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
     1681 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
     1741 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
     1801 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
     1861 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
     1921 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
     1981 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
     2041 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
     2101 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
     2161 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
     2221 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
     2281 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
     2341 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
     2401 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
     2461 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
     2521 ataaactatt taacttttag aattcaaaaa ccgtttgtct atttagagtt taccctaaat
     2581 agacaaaaat ataacattga aaaatttaga gttctaacgt atgattaggg tgtattataa
     2641 acgttataga aaagtaggga ataaatttga aaattgctta tattagagtt tcatccatcg
     2701 atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta
     2761 tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa gctttagatt
     2821 ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga cgcaattatg
     2881 acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt attatcacca
     2941 gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc atcaaagact
     3001 taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg aaacgacgcc
     3061 aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt cctaagttat
     3121 acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc gttcaggatc
     3181 ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact aggcaaacaa
     3241 tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt tgaatcacgt
     3301 aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc tagctaagtt
     3361 gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc ataaatcgtc
     3421 cttatttaaa ggcatttaac attgacaact tagtataatg tatcttatgt aaactaataa
     3481 gatttgaatt ttattttata cacactcgat aatagatcaa ttttatggtc ccccgcattt
     3541 acgattgcac aagagaaaca taaagtcgac ctattagcat ttcttttcaa ttcaaacatt
     3601 aaaaaagttt atacctctgc tactaaaaaa tgaaattatc tatttaaaat ttaaaacggt
     3661 aaatctgatt catccaaatt ggaaaatgga tcagcctgta ttggagcttt gtatttctcc
     3721 ataataacag atatcttttt ttcatttttt aaaaaatacc ttttaaattt cttatagtcc
     3781 tctgaaatca aaatatagcg tgatggatac tcatcaaaag aagaatcgta ttcttccttt
     3841 aaaattcccg atttacttaa tttttctagt acatcaaata ctctatcact caagtaactc
     3901 cttaaattgt tatcactttc ccattgaact acttttctat aagttgaatt atcagttgaa
     3961 aagatagaat tttcatccat aaacgcatat attagcagca atctttcatc attgtcaaat
     4021 tctattggat caaaaatatc taagttgtca tcaaaatctt tatcagttcc ggtaactttt
     4081 ttgttaatta ctgaaggtgt cttgtaagaa atagaatcta aatcaaagcg ttttgattct
     4141 aaattatttt gtgctttctc aattaaataa ttcacttttt ttgtgagaaa ccttctgata
     4201 ttgtctttgc tatatgttcc ataaaaaccc ttatcttcta aagagtccct gtacttctta
     4261 acttctatat actcttcagc caaaacttgt ggaactggtt catctaagaa aaaaacccct
     4321 acctcgcctt tgctattttt ataaaagtaa tcaatttctg ctatagttcc cgatctataa
     4381 ttatcaactg gagaacccaa cttatggcca aaaatcgcta ataatatatc actattttct
     4441 acaatttgtt tattaataac actttgccca gagctattat ttatataagt aggaatcgca
     4501 ttcgtttccc atcgaattgg catcaaaatt acttttgtat tatctgtatt ttctatattc
     4561 catctatata tggtttcttc tacttcattc ctctttgctg ctatatcacc tggtgatgct
     4621 ataaatactt ttattagatt tatggttttc atttattgta cctttctctt tatgtctctt
     4681 taaagtaatt tttgtataaa atttattgac ttttacacta taggacaact tttatctatt
     4741 tagtacaaaa aggcccacta aactaattga ttagttagga gcctattaca ggtatatttt
     4801 caactcgctt ttaagcaaat tctactacca gctttttgcc taatgcccta gcgatatcat
     4861 caagtttatc gaaagatacg ttatcccctc tctcaattcg agcaatagta gattgagttg
     4921 tatgtgcttt ttgtgcaagc tccgcttgag ttaggcctgc ttcctcccgt gcatgataaa
     4981 gagcaaccgc agtttcgagt tttcttcctt cacggtcata tgcttctgca aaatctttgt
     5041 ctttttgctt ttcttcaatc aattttttaa tcatcatttg tgaaccactc ctctctaagt
     5101 tcttttgcat gtttaagttc acttattggt gttttctccg tttttttagt aaatccatgg
     5161 gtaatgacat accttccatt gaccacatgg aaatagatag ctctttggat gtttgatcct
     5221 accttacttc ttaattcgta caaattagtg tctaattttt tcacccattt caaccgttga
     5281 gctactagca tcccattctt ttctgtttct tctattgtac gtaaaagctt cgcactatct
     5341 ttttttggta atgtttccaa ccattctaaa aattcatcat gaccattagg tcttttaaat
     5401 gtttcaaaag atattttttc catacccaat tatatcatat attgatttta tattatagta
     5461 tataacataa aaattcgaat t
//
LOCUS       DANJMI010000107         5312 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000107 DANJMI010000000
VERSION     DANJMI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.111"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="PWU81_002951"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="PWU81_002951"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL1111566.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="PWU81_002952"
     CDS             complement(1297..2133)
                     /locus_tag="PWU81_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1111567.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="PWU81_002953"
     CDS             complement(2317..3534)
                     /locus_tag="PWU81_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL1111568.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3862)
                     /locus_tag="PWU81_002954"
     CDS             complement(3683..3862)
                     /locus_tag="PWU81_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111569.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3917..4000
                     /locus_tag="PWU81_002955"
     tRNA            3917..4000
                     /locus_tag="PWU81_002955"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..4818)
                     /locus_tag="PWU81_002956"
     CDS             complement(4240..4818)
                     /locus_tag="PWU81_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111570.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(4824..5207)
                     /locus_tag="PWU81_002957"
                     /pseudo
     CDS             complement(4824..5207)
                     /locus_tag="PWU81_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1654 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca ta
//
LOCUS       DANJMI010000108         4933 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000108 DANJMI010000000
VERSION     DANJMI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.112"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            85..972
                     /locus_tag="PWU81_002958"
     CDS             85..972
                     /locus_tag="PWU81_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL1111571.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="PWU81_002959"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="PWU81_002959"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL1111572.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="PWU81_002960"
     CDS             2274..2720
                     /locus_tag="PWU81_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL1111573.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="PWU81_002961"
     CDS             2993..4345
                     /locus_tag="PWU81_002961"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HDL1111574.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="PWU81_002962"
     CDS             4516..4803
                     /locus_tag="PWU81_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111575.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DANJMI010000109         4826 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000109 DANJMI010000000
VERSION     DANJMI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.113"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(4..1203)
                     /locus_tag="PWU81_002963"
     CDS             complement(4..1203)
                     /locus_tag="PWU81_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL1111576.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1333..2709)
                     /locus_tag="PWU81_002964"
     CDS             complement(1333..2709)
                     /locus_tag="PWU81_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1111577.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2709..3365)
                     /locus_tag="PWU81_002965"
     CDS             complement(2709..3365)
                     /locus_tag="PWU81_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL1111578.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3375..4652)
                     /locus_tag="PWU81_002966"
     CDS             complement(3375..4652)
                     /locus_tag="PWU81_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL1111579.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4671..>4826)
                     /locus_tag="PWU81_002967"
     CDS             complement(4671..>4826)
                     /locus_tag="PWU81_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDL1111580.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1512 a          878 c          580 g         1856 t
ORIGIN      
        1 tttttagaca tcatagctat cactccttgc attcgttttc ataaatctat tataaaagaa
       61 aagactgtag acatcatcca taaaatggac tttgtctaca gtctgcgcat tctcattaga
      121 gaacactccc ttttttgtga cttttctgaa gctagagtga atactttcaa ctgcattagt
      181 agtgtacatg atttttctaa tggcgctgcc ataatcaaag agttgttcaa catgagaaaa
      241 gtttcgtttg catacatcta ctgcaccaga ataatgagac caccgatttt gaaaactgtc
      301 gaaggcagcg tgtgcagcgt ttaaagaaga agcactgtag aattttttca tgtctcgaca
      361 gacttcctta taatccttac ttgggaggta tcgtaacgca tttcgaacaa gatgaacaat
      421 acagcgctgc acaatgacag aaggaaaaat tgcttttgcg ccttctttga gaccggaaac
      481 accgtccatc gaaatgaaaa aaacatcttc tacaccacgt actttcagtt catcaaatac
      541 ttgcatccag cgatttttag attctgtttg attgagccat aggcctaaaa tctccttgtt
      601 tcctttaaga tcatagccaa gaatggtgta cacagcatat tctttagctt catagttttc
      661 gcgtaaagtg acgtacatac aatcaacgaa tagaaatgca taacacttgg ctaatggccg
      721 agcttgccat tcttccaatt caggaaggac ggcatccgtg atatcagaaa tcatttcgtg
      781 ggaaatatca aagccgtaaa tatcttcaac ggttgcggcg atatcgcatt gactcattcc
      841 tcgtgcatac atggaaagaa ccttaccttc gatgtcggag acatctcgtt ttctattagg
      901 aatcaactca ggttcaaaag aagcctcccg gtctctggga acatcaatgt ttactttacc
      961 aaagctcgtt ttaagcgtct tattgccata accgtttcgt cgattgtcat gtttctttgg
     1021 ctctttagaa tgggcgtcat accctaaatg gttattcaat tctccttgaa gcattttttc
     1081 aaataaggga ccaaacacat cttttaaaac atcttgcata tcctctacag attcgggttg
     1141 ataagtattc aaaatggatt cagctaactt ctcagcatca ggatttcttt tctttctagc
     1201 catcgtgtaa tcacctcttg atcttatttt agaaaaataa aaaccgtgaa gcaaccaccc
     1261 gcctaggatt gatggttact tacacggttt atattacact ctcatttttg gtgctaaaaa
     1321 gcaccgaaaa ttttaattat tgtcaattaa taattctttt ggattccttc ttaatatatt
     1381 gaatgaagaa ataatcaatg atacaagaag tactaaactc ataaatataa ctacataaat
     1441 catgaattta ggtaatacat tgatatctaa acttgataat gtcttgttaa atccttcagc
     1501 ttctgcccca ccacctaatt ggctagatgc agattgttta gctatttgtt tagctatatc
     1561 accagtaacc ttattcaata tattattccc aagcttgtca gctgtatatt gagctaagaa
     1621 ataggaacca acgaatgcgg ggattgatat aaataccatc tcaattagga attgaccaaa
     1681 aatttctaat tttgatatac ctaatgatag taatactgct atttctttct ttctagcatt
     1741 catccataag aataataata atgaaactac aactcctgca aatattaatg agccaaaaaa
     1801 taatttattt gaaattgagt aaatacctga tatagattgt tgtaatgcag ggtaatttga
     1861 tgaactttta atcaaattat attctctcca atttatatct aatttcccaa gatcttttat
     1921 tacactatca agattcttat caccttttac aaagaatgtt gcatcttggt atacagctgt
     1981 atcttcggta ttaccataaa ctttagcagc tgtatgaata tcagtgatta atgtattttc
     2041 gtatagttct tgtgctgcac ttactccacc gctattatgc ccatcgaata gacctttaat
     2101 ttctacttct actgtttcat ctgcaccttt ctcattatca gcatcaaata aattagattt
     2161 tatttttatc ttatctccaa ctttaaggtt atttttttca gctaaatctt tatgcattaa
     2221 gattttattt ttatcttcat tttctaaatg ctttccttct actaatttat atgcttctga
     2281 tacaaatttt gtttcttttg ttgagtcgtt aaccccagtt aacataactg ttcttttaaa
     2341 attctttgct ctttcaggtg attgattcgc aagagtctct tgagtttcaa taatatcata
     2401 atcaactaaa tctgcaacac tgtttattct ttttacatag gagtctatac tatctgtttg
     2461 agatatcttt ttaatatctt caccttttac atttccccca cctctaggtg ttcctggatt
     2521 tacttgtcta tttatctcca tagaaaaact atttgttata ttagcaaatg tttcttttga
     2581 agctctgtcc gtagcatctt taatagataa actaatgaga cttaaagttg acatagataa
     2641 aataactaat atgataatta atgattttaa actttttcta gttacataag caaatgcatt
     2701 ttttatcatt attcaacctc caaattcatt ttatttactt ttttcaactt tttaccactt
     2761 agttctaaaa tgatatccgc agaatctgct acttccttac tatgtgttac aactattaca
     2821 catttatttc tatctttagc taatgtcttt aatatattaa ttatttctcc agcagtaaca
     2881 ctgtctagat taccagtagg ctcatcagct agtattattg gagcatctga taccaatgct
     2941 ctagcaatag ctactctttg ttgctgtcca ccagataatt tcataacatt tctttttatt
     3001 tgttttttat ctaaacctag ttcaaacaag atactttcat ctgctgattt atttactaat
     3061 ctaatatttt caattggtga taaataatct attaaattat agttttgaaa tactaaagat
     3121 atattatttt ttctatgatt actatatcct ttattttgta tatcttcatt cttaaacaat
     3181 atttttcctg tttgaggttt atcaagtcca gcaagtaagg aaagaagtgt agattttcct
     3241 gttcctgact ttcctactat cgcataaaac ttcccaagtt caaatttttg atttactcct
     3301 gacaaaactt tttctttaga atttgcataa ctatatgcta cattctttat ttctaatata
     3361 tccattattt tctcctaact tatttttgat aagatttctt taggcttctt gattaacatt
     3421 aatgaagaag caaatacaac tgataaaaca ataatactta ttaatattaa ataactttgt
     3481 ccaagtgttg ttatatttga cataaaacta ctattattta ttaaacttcc accggaaatc
     3541 attgaatcct ctgagttaat gaatccatct acaattactt ttagtaatac atttcctaaa
     3601 aataaggaag atattatact tggtatcgat atgaatatta actcgaatat aaattgcatt
     3661 ataatttgta tcttagatgt tccaatagat aagaatatac ctatttcata aattctttcc
     3721 cttaaccata gaatcaagat taatgaaaga acaaccatcc cacctaacat aatagaataa
     3781 gtcattactt ttattatata ttttattcca ctcactgact ctaaagactc ttcaaacgca
     3841 ttagaatctt tttcaacaaa atactttgac tcatcaattt taagctcttt caatttgttt
     3901 aaggctaagt ctgtagattc tgcactacca gaatacatta aaattttatt tgcaatttca
     3961 ttattttctg aattatttaa tatctcttgg cttgttgaat aatctacaaa caccatattt
     4021 tcactaaaat cagaagataa tcctgtatat gtttcctgtt ttttaccaga aaagatccct
     4081 ataattttaa atttatgact ttttattttt ccactttttt caatatctag taattcaaga
     4141 tcaacttcat cacctaattt tagattgttt tgtttagcaa attcttcatg aacaataatt
     4201 gaattcttat cattttcttc tatattttta ccttctttaa ttgtaaatac tccactacta
     4261 aataaaatat ttcttttagt attatttgta gcttcgagtg aaacaacatt cttaaattcg
     4321 tcagataaat cttctctatt tattctttgt tctccgctaa ctactttagc atcttttagt
     4381 tttgctaatc catcatattg aattattttt tcttcaattt cttttaattt ttcaatatct
     4441 ttaaattgat taacattaaa atatttacca tctttttttg ttattgatat tgaagaatta
     4501 gaactttcat ataaagcctt ttctatttca ttgcttgatt tcattattgt taaacaagaa
     4561 tacaagcaag aaagaactat agttaatatg ataaaaatta ttaaagttct atttttcttt
     4621 cttgtaatat atgctatagc attttttatc actttcctaa ctactcctct ctattttctt
     4681 actccataac taattgcatc cactggacaa gcatttttac atcgtccaca tctgatacac
     4741 tccagatgat tgcaattctc aactgaatct ttattcattt ggcaaacttt tttacatttt
     4801 ccacaattaa tacacttatc cttatc
//
LOCUS       DANJMI010000110         4680 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000110 DANJMI010000000
VERSION     DANJMI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.114"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(<1..1537)
                     /locus_tag="PWU81_002968"
     CDS             complement(<1..1537)
                     /locus_tag="PWU81_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL1111581.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAEL"
     gene            1721..2383
                     /locus_tag="PWU81_002969"
     CDS             1721..2383
                     /locus_tag="PWU81_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL1111582.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            2527..3090
                     /gene="ahpC"
                     /locus_tag="PWU81_002970"
     CDS             2527..3090
                     /gene="ahpC"
                     /locus_tag="PWU81_002970"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDL1111583.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            3100..4629
                     /gene="ahpF"
                     /locus_tag="PWU81_002971"
     CDS             3100..4629
                     /gene="ahpF"
                     /locus_tag="PWU81_002971"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDL1111584.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
BASE COUNT         1498 a          855 c          920 g         1407 t
ORIGIN      
        1 caagctctgc ttggtaaaga tctacaccaa aaatttcttt atttgacaaa ataggagcca
       61 ttttctcatg aatatgtgat gtatcttgcg tacccagttg aatatcggaa agcttatgct
      121 gaaggtctgt caatagtggg tctggactcg ggatgaatgg ttttccttga tcatcaatgc
      181 caataaggta tcgaagccaa gcagcgagag tcaatggtat gaatacaagt gtctgcgggt
      241 ctttaacttc tatttccata tattttttaa tcgtctcacc ataacgaatc gccacctttt
      301 gagaggtatc cgtggcgatt cgctgaggca tattaggaat atttggatta gaaaagcgca
      361 cagtaattac ttcttccaga aagatctttg gatcaatgat acctggagac tccacaactg
      421 ggaggccttc attgtacccg atttcagaaa ctagcagcga taaatctgga tctttcatct
      481 cctcagctat gctcctatac cccagtaaac aacccataat agctaaagac gtatgcaaag
      541 gattaaggca agtcgtcacc ttcatcgtat ctgctttatc taccgttgca cgatcagtca
      601 agaatacccc agctttttcg aatttcggac gtccattggg aaaatgatct tccatgatta
      661 aataatggat gtcctctgta ttgacaaatg gagcaatatt atttcctttt gcggtttgga
      721 aaatcgacat atttgaaaag ccctcttttg ccaacatttt gctaactgac aaggaaggat
      781 tcggggtgat acgatcaatc atcgtccatg gaaacgcaac aatttcttca ttcgataagt
      841 attggataaa tgtattttgt acataacctt ccttttgcca ttcttttgcg atcgttagca
      901 aagaagtacg taattgacta ccgttttgtg aaaaattatc tgtagagaca agtgcaatcg
      961 gccaagctcc agataaaaaa cgtttataaa gtagcacagc aagaatactc attgtatgtg
     1021 atggcttctt tggaccattt tttatatcga ctgcaatcgg tcctatatat ttattatttg
     1081 tatctttcaa tcgataacct ttttcagtaa tagcaaaagt aactaattgt aaactaggat
     1141 tttcaaagtg tcgaatcatc ttatcaaagt gttcagaaca atctggatga caatacagac
     1201 tgtcagctgt cgcagctaat atacgtttga cgaactctcc actgcttggc atgaccactt
     1261 caagaatcaa atcatcaaaa tcttggtaag ccctttcaat tatttcttca tcgaatgttt
     1321 cacaaacaat gacaccagtg gacaattcgc ctagatccgc taaacgttga gcgatttctg
     1381 catgaaatcc tcgataaaga tttccgccac cgaaatggat ccagacagga tttttttgtc
     1441 cattttctct taatgatgca cggtcaaaat gaggtatttc tacttctttt tgagataatt
     1501 cagggataaa gttcctccag ttgtttaaat caatcatttt tcccctccta aactaacata
     1561 ttagtttttg tattcaagtg aattttatgc aataaaaatc gctatgtcaa ccaaaatttt
     1621 actatttata caaatttact agttaatgtg acataccaga ttgtttttca gacctcttct
     1681 gttatactac gttcatagaa atgattttgg aggaataaag atgaaaagca gcttgcaatc
     1741 acaagcttat caagcgattc gtaaacaaat tatctatgct gacttagaac ctggaaggaa
     1801 aatatctgaa aaggcactgg aggaacatct tgctatcggc cggactccca ttcgcgaagc
     1861 attgattcaa ctacgtcagc aagaactcgt gtatacgata cctcaaagtg gtacgtatgt
     1921 ttccaaaatt aatcttgttt ctgcagaaaa tgctcgcttt gtccgagaac agttggaaga
     1981 gcaaatcatg attgaatgtt gttccaaatt ggacacacaa acacgaatag tgctagaaac
     2041 gattttagaa gaacaagaaa aagcagtcaa aataaatgac gaacgcgctt ttttccgtgc
     2101 agataattta tttcatgaag cttgtttcga aattgcagga agaaaagaag tttggaactg
     2161 gttagatgac cataataccc atttagaacg tttccgctgg ttacgagtga ccattcaggg
     2221 gcttccatgg gaaacgatca tggaacaaca ttatgattta ctcaaggctt tagttgataa
     2281 aaaaccagaa gaagctcgat ttcaaacggg attgcactta cacatgatgc tgaaagaaca
     2341 ggaaactgtt actcaacgtt atcccgagta tttccagcag tagcattttc ttgcgacctc
     2401 ttctattttt ataattaacc ttcgtttttg agtaatcttt attaaataac aaaatatcca
     2461 ttgactttgc aactccaact aaattacaat aattataaat aaataattat tattggaggt
     2521 tttactatgt cattgattgg gaaaaaaatc gaagaattta atgcacatgc ttatcatcaa
     2581 ggagaatttt tagaagtcag tgaaaaagat cttatgggga actggagtat tctttgcttc
     2641 tatccagctg attttacttt tgtttgtcct actgaattag aagacttaca agaacaatac
     2701 agtacattga aatctttagg tgttgaagtc ttttcttgct ctaccgatac ccactttact
     2761 cacaaagcat ggcatgatac atcagatgct atcggtaaaa tcgaatacgt gatgatcggt
     2821 gatccgtctc atcagatttc tcgtattttt gatgttttgg atgaagagca aggattagct
     2881 cagcgaggaa catttattat tgatccagac ggaatcgttc aagctatgga aatcaatgca
     2941 gatggaatcg gtcgtgacgc tagtgcactg attgataaga ttcgcgctgc tcaatatgta
     3001 cgtacccacc caggtgaagt ttgtccggca aaatggaaag aaagtggcga aacattgaaa
     3061 ccaagttttg atcttgttgg aaagatttag gtggtggata tgttagacaa agaaactaaa
     3121 caaaatctcg aacaatattt agcactgatt gaatctccta ttgttttttc tgttagtttg
     3181 gataatagtg aaaatagtca aaaactagct gaatttacga aggaaatagc agaaatgtct
     3241 cctaaaatct cttgggaaaa aagagattcg atgcgcacac ctagtttttc tatcaatcca
     3301 gttggcaaag aaagtggaat cgtttttgct ggtatccccc tcggtcatga attttcctct
     3361 tttttactgg ctatgttaca aattagtgga cgggcaccga aaatcagtga atctttgagt
     3421 cttaaaatca aaaaaataac acaaacattg cattttgaca gttatgttag cttgacttgt
     3481 cataattgcc ccgacgttgt acaggcattg aatatattag ctgtattaaa tccaaatatt
     3541 actcatacga tgattgaagg tgggatgttc caaaaagaaa tcgatgataa aaaaatcatg
     3601 gcagtgccaa ctgtgttttt aaatggagaa gaatttagta gcggacgcat gacgatcgaa
     3661 caaattgttg aaaaaatcac tggccctttg attgaagaag agttgtttga aaaagaacct
     3721 tatgatgtat tagtgattgg cggtggacca gcagctagta gtgcagctat ctatgcagca
     3781 cgtaaaggaa tccgtacagg acttgtcgct gattctttcg gcggacaagt cgttgaaaca
     3841 ctagggattg aaaatatgat tggtacctct tacacagaag gtccaaaatt gatgcagcaa
     3901 gtagaaagtc atgtgcgtag ttaccctatc gacattatga tgaatcaaca ggccgtttcc
     3961 ttaaataaag agcaacattt tattaatatt ggcttagcta acggtatatc acttaaagca
     4021 aaaaccgcca tcatcgcgac aggtgcgcac tggcgttcta tcaatgtacc aggcgaaaaa
     4081 gagtttaaaa ataaaggaat tgcttattgc ccacactgtg acggcccttt atttaaagga
     4141 aaagagattg ctgttatcgg tggtggtaat tctggtgttg aagcagcaat cgatttggct
     4201 gggttagcaa aacatgtata tgttctagaa ttcttaccag aattaaaagc agatcaaatc
     4261 ttacaggata aactagtctc tcttgcgaat gtcactgtta tcaaacatgc agcgacgaaa
     4321 gaaatcagcg gtacgaatca ggtagaatct ctctcctata tcgatcgccg tacgatggag
     4381 atgcatacac tagctgtttc aggagtattc attctagtcg gcttactccc taatacggat
     4441 tggctagatg gaacgatcga aatgaatcca agaaatgaaa tagtgaccga gaaaaatggc
     4501 gcaacgaata tccccggcgt tttcgcagct ggtgattgta cagatagtcc atacaaacaa
     4561 atcattgttt caatgggttc aggagctact gcagctttag gagcattcga ttatctgatg
     4621 cgcacttgag ctatgaagat tgtaaaaagt cagagagcgg aaaaaaggat ggtgacaaga
//
LOCUS       DANJMI010000111         4615 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000111 DANJMI010000000
VERSION     DANJMI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.116"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(1016..1306)
                     /locus_tag="PWU81_002972"
     CDS             complement(1016..1306)
                     /locus_tag="PWU81_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111585.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1708..1944
                     /locus_tag="PWU81_002973"
     CDS             1708..1944
                     /locus_tag="PWU81_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111586.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1951..3903
                     /locus_tag="PWU81_002974"
     CDS             1951..3903
                     /locus_tag="PWU81_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL1111587.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3975..4472)
                     /gene="dfrG"
                     /locus_tag="PWU81_002975"
     CDS             complement(3975..4472)
                     /gene="dfrG"
                     /locus_tag="PWU81_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL1111588.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1582 a          811 c          756 g         1466 t
ORIGIN      
        1 acatacttgc aaagtaatac tacaattcct aacataatcg tagaatttaa taatttccat
       61 agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct tgaactccat
      121 gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct acttgcataa
      181 actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa gataccccaa
      241 atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt atgtttgttc
      301 atttttatac tccttcttat atttcctaat agagtataac atagctttaa gcattactta
      361 aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat ttgatgcaaa
      421 agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt tttcttagtt
      481 aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa gacatacaat
      541 catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa aatccaaatg
      601 ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac
      661 ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca ggaacgttta
      721 aaatcctaca attccactac ctgagactag taatgactag tctcttttta tggcctctga
      781 tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc tttaagcaat
      841 atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa tacatgtagt
      901 tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg
      961 catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt gttttctagt
     1021 ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca tattctacca
     1081 caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt ctattattat
     1141 aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag ttcaaactat
     1201 gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt ctggcactaa
     1261 cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc ttcacctcaa
     1321 taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta tctttttgac
     1381 tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat gaaggacatg
     1441 tactaaaaaa aagaggcagc agtatagatt taagtagttc agttttggag tacaaaaagt
     1501 gaaaatgtaa acctaaatga gtatcaaatt gcaactgaat ttgatactcg ttttttattg
     1561 ttaaatcaac gtttgttgtt aatttacacc acaaaatgta agttaaaatg tatataaaaa
     1621 tgcgaacgtg aaataattat tgtaggaatg caaattggtg tgttaaaatg gttacaattt
     1681 actaagcaaa aaggggtgca ttttcctatg caatttcttg agccaaaaaa taagaacgct
     1741 aagtctgtgg attgggaaat atcagagcaa gtaagggtga tagttaagca atacgctgaa
     1801 tatgcagagc gtactgaaag cgaagcagtg gatgaattct tattgaacat tttagacgat
     1861 aagaagttta ttgaatggat tgccaacaag cgaagtaata agcggattgt tgaaaaaatg
     1921 ggtatcaaag atagagtggg ttgatagaca atgaccaagc ttaaaagatt agccacgaag
     1981 gaagatgaaa ttgtagatgt taaaattccg atttcaaacg aagaattaaa agatagggct
     2041 aaacaatatg atttattaac tccaaaacga tttgcaacaa ggtataacaa aatgttgttc
     2101 ttgccaacgt cttttaaatg gaacggttct gaatatccca ttcaatataa ttactgcatc
     2161 aatccgtttt gttgtaattt tgggaaagaa cagcacaaat tcaaagatgt aaaagggaaa
     2221 ccaagtcgat ataaaatgac tggtagttcc aaagataaag gtcataaagg tatgtactgc
     2281 aatgacaatc ctattggcag aggtgtatcg caaaactgta cagtaacccc tttatctaat
     2341 tggtctgttg ttgaagaaat taaacgcctt atagaaatca atagcatcca agatgttgag
     2401 ccagattatc aatttcataa agaaggctgc tcggaagaag aatcaacccc attcaatgag
     2461 ccgaagcaat tttacaaaag ggggaaaagt aggggcaaat ctcaacgcta tcaatgtaag
     2521 gcgtgtaaga agttcactaa tgttctacca aaaagagaag aaaccactac ttatcaccaa
     2581 caaaagaaca ccattcttcc aatgttcgct aagatggttg ttggaagggt ttcagtcagt
     2641 cgtacttgtg atattttagg aattggagtt ggaacctact atcataagct ggagtggctg
     2701 tataggcgtt gtttggagtt tttagaacga tatgagacac aaccccttca aacaaagaaa
     2761 tttaacgaga tgtggcttaa tacggacaag atgcactact acctaaataa cgtccgtaag
     2821 aaaggacaag gttcaaagaa atatacgggg tttgaagacc ttaatatgca aacgtatata
     2881 gttgtatctg ctgaggtctt atcaagatat gtgttccgtt ctgatgttgc ctatgattgg
     2941 aatatttcaa tggatgaatt aaatgaagat acaagaaaat tcaaagaaga ccatctcaat
     3001 acttttagtc gtaaaaatga taggttagat tggtcttatt atccacaaga accatccgct
     3061 aacgatagcg aaaataggaa tgcttatctt cacgaattag ggaaaattac taataggagt
     3121 agatttgtcg atggtttaaa tgttgatgct ccttatacta caactgctca ctattggtta
     3181 attaaacaaa tggttaatgc cgacgaatgg cgaatgatta gtgatgatga cttctctatt
     3241 agaaacgcct tttatagagt attcacgaaa gaattaaggt tgagcgatgc acatcatttt
     3301 atttgccaag ttaacaagac gaaaagcaga aaacaatgct taaaagaatt cggacaagca
     3361 aaagcagaac tgctcgattg gggagatatc agaggattca aaacaaagtt tctacggact
     3421 ttggcatctc attatctaac tgaattacta acaagtcatc aattccacga agaggctatt
     3481 agtaaagatg gagaaagata tcgcaaatat gccgataacc ctatcaaaca tccattagcc
     3541 actaaggata aaggatttta ttcagttgat tgtagaacgg atttatctgc tctggaacca
     3601 aacgaaatcg caaagatgct attaaatgtt aatgaccatt cgaccaatag ctttattcaa
     3661 cagataagac gttatatatc atctcttgaa agaccattaa caacagctcg tggtgataag
     3721 aagagctata tttacgctaa cttcaatccg aagtatgctc aatttgcgat tacaattcta
     3781 agaacttact acaacttttg tcgccctttt aaatcagcgg ataaaaaggt tttgacacct
     3841 gctcaaagat tagggattac agataaacaa tttgattgga aagatataat ttatttcaaa
     3901 tgacaaaaag agcttggagt aatcccaagc tcttgtgctt tatctttact gttcttcttc
     3961 aattaacgca cccgttaact caataagttt tttctttcat atacatgaaa atagtagtta
     4021 tacggatttt tatcattctt tatccctttt tgggcaaata cctcattcca ttcctcataa
     4081 ttcacttctg gaaaaaaagt atctccttcg aattcatgat gtatttttgt gatgtacatt
     4141 ttctcaacat aagggaaaaa caaattataa atctgttctc ctccgaaaat aaaaatttct
     4201 tcttcgtttt tacataactc aaaaacatct tctattgaat gaacaatttc acaaccatta
     4261 aaggtaaacc ccttatctct cgtcagaata atatttcttc tgtcaggtaa ggctcttccg
     4321 attgattcaa ggttcttcct acctaatatt atcggatgtc cctttgtagt atttttaaca
     4381 tattcccagt ccttgggaat cctccaagga atgtcattct ctttgccaat cactctattc
     4441 ttatccatcg cagcaatcaa agaaactttc atttataaca cagccttttt aactaattta
     4501 ccatctaatt tcttatttct atcaaaaaac cttttctcat tatcctgccc gttagttgaa
     4561 taagcaattt tttctataag ttatattatc ataataccac aaaatcacaa atttt
//
LOCUS       DANJMI010000112         4587 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000112 DANJMI010000000
VERSION     DANJMI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.117"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            186..2855
                     /locus_tag="PWU81_002976"
     CDS             186..2855
                     /locus_tag="PWU81_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDL1111589.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3566)
                     /locus_tag="PWU81_002977"
     CDS             complement(2946..3566)
                     /locus_tag="PWU81_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL1111590.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="PWU81_002978"
     CDS             3680..4219
                     /locus_tag="PWU81_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111591.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGEMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="PWU81_002979"
     CDS             4216..4554
                     /locus_tag="PWU81_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HDL1111592.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1420 a          790 c          859 g         1518 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggagaaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgac
//
LOCUS       DANJMI010000113         4465 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000113 DANJMI010000000
VERSION     DANJMI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.118"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            574..1008
                     /locus_tag="PWU81_002980"
     CDS             574..1008
                     /locus_tag="PWU81_002980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111593.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="PWU81_002981"
     CDS             2298..3221
                     /locus_tag="PWU81_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL1111594.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="PWU81_002982"
     CDS             3237..3776
                     /locus_tag="PWU81_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL1111595.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1667 a          674 c          757 g         1367 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacggagaa cttaaattaa attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagagacg gataaataat aaaaaagact
      181 gctctttcga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tatgaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacacccca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttga cttagaccaa tcgaaaatca caaagtgagc ccctgtatcc cttggtagag
      421 aggctacttg gcatagtaca cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct ctcaacgtgg taaaaatccc acttataaga gaaaatggtc aagaacctaa
     4261 acggtcggcg aagaaaacat accaagcacc acaagcaaaa aaagttagag taacttccaa
     4321 aaaagaaaaa ttttttagaa caacttttga aaatttaatt ttgatttata tccgttcaat
     4381 ttaaacattt gataaactga aggctagtat ttaaaatgtg tatacaagtg gtagcgttaa
     4441 aaaaagtgga ggtataacct atgtt
//
LOCUS       DANJMI010000114         4433 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000114 DANJMI010000000
VERSION     DANJMI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.120"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(543..2048)
                     /locus_tag="PWU81_002983"
     CDS             complement(543..2048)
                     /locus_tag="PWU81_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL1111596.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="PWU81_002984"
     CDS             complement(2045..2731)
                     /locus_tag="PWU81_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1111597.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="PWU81_002985"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="PWU81_002985"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDL1111598.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1238 a          919 c          730 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa agggatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DANJMI010000115         4335 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000115 DANJMI010000000
VERSION     DANJMI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.121"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            127..579
                     /locus_tag="PWU81_002986"
     CDS             127..579
                     /locus_tag="PWU81_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111599.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="PWU81_002987"
     CDS             593..1282
                     /locus_tag="PWU81_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111600.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="PWU81_002988"
     CDS             1407..3758
                     /locus_tag="PWU81_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HDL1111601.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="PWU81_002989"
     CDS             3838..4233
                     /locus_tag="PWU81_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111602.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1526 a          548 c          867 g         1394 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatt
//
LOCUS       DANJMI010000116         4205 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000116 DANJMI010000000
VERSION     DANJMI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.122"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            994..1962
                     /locus_tag="PWU81_002990"
     CDS             994..1962
                     /locus_tag="PWU81_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL1111603.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            1979..2503
                     /locus_tag="PWU81_002991"
     CDS             1979..2503
                     /locus_tag="PWU81_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL1111604.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            2879..3160
                     /locus_tag="PWU81_002992"
     CDS             2879..3160
                     /locus_tag="PWU81_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111605.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            3162..3578
                     /locus_tag="PWU81_002993"
     CDS             3162..3578
                     /locus_tag="PWU81_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111606.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1606 a          735 c          693 g         1171 t
ORIGIN      
        1 aggtatttgg ctagcaaata tagagcttgc caaacacttt agtgagtttt gtagaaagta
       61 cctttaaagg tactttcaca ttacgaacga tacgtccgta ggacgtactg aaagggtggg
      121 atttatgagc gaactaatgc aaaatttggc tagcaaaaaa agttaaaaaa tatatgctaa
      181 taatcgagcg aaatgaacac tttttcaagt gagcgaatcc gccatgacaa agctgaagca
      241 gtttatttca gctttgtcat ggcgtgtgcc tagactttta aaaaagtcgg cacacgagca
      301 aatatcttta tcaagctcta atttgcgttt taagacgttt gaaacgtcta agtatataaa
      361 tatataagag taggtttaag agtgcttaaa tcgaatttta gagggcattc agacaacttt
      421 agggttgtta gggcttgccc tgaccgcctg taaggcgctt taagaacaag ataagggatt
      481 tatactaaga ctgtcttaaa acgtctcaaa cgaaggctca tagcttctca aagagttcct
      541 tatgaacatt tttagcaaag gagccggaag cgactgcgca gtataatgtg agagcagagc
      601 gatcacacct ttttaagcga ctgtagggag taagattccc agttgagaaa aatgcgcgaa
      661 tctgtttgga gaaaaaagaa gtggtttttt aggcgggaaa ttttgtcccc ctcttttata
      721 atctcttttt aaacctcttt ttaaacctct ttaccgtcgg ctatatcctt actctcccaa
      781 gggaatgacg cttttatcta catcgttttg tctgtttatc tacatcgttt tgtctgttta
      841 tctacatcgt tttgtctgtt tatctacatc gttttgtctg tttgtacatt gaattagtac
      901 atcgacgatg ttaatataag atgtagataa acaggcattt tgtatttcat aacgtgtatc
      961 tcttgtgcga gtaagaaagg agctatttta aatatgtcta atgaaatcgt aaagtatcat
     1021 catgaattaa atacaattcc gttgcgaaaa tttacaccag tcgaaatgaa tttgttcttt
     1081 tctatcgtat caagaatgcg tgatgtaggt gataaaaaag tacaatttac attcgagcaa
     1141 ttaaaagatt taagcaatta taaagcaact gcaaatgttc gattcataga tgatttagaa
     1201 acaacgtatg acaaattaat ggatttacgt tttggtagaa gaagtgctga cggtttgcaa
     1261 cgtgaacgtt ttgttttatt taatcaattt aaaatcgacg gaaaatctga tattcctttt
     1321 gctgaaatac aagttcatga aaaagcctta cccttactta ataatcttga agaatgggtg
     1381 cgctattctc tccaacaatt caatgaatta gaaagcagtt attcaaaaac aatgtttcga
     1441 ttactaaaac aatacagaac caaaggtttc gcttattttt caaaagaaga ctttcatgaa
     1501 ttacttgata ttcctaaaag ttacaaccaa ggtacgattg atcaacgtgt gttcaatcca
     1561 atacgacaag aactaaccgc aatctttaaa ggtttaacaa tcaaaaagaa atacggaaaa
     1621 ggacggggaa agcctgtcat tggctatcaa tttacattta aacctgagat aaaaaaatca
     1681 gacgacttta acaaacatgc cccaacaaaa gaagaacaaa acaaagcatt agaactagct
     1741 aaccaacaag ttcaatctga aaaagcacct acttcattac aagaaaaaat caaagaacaa
     1801 aagcgaaaat tggaactttt acaacagtta gaaaagttag aaagagaaca aaatacccaa
     1861 aataaagaaa ataatgcaca aaaatcagag ataaattccg atattccttc agaactaaaa
     1921 aaagacttat tagacaatct tcaaaactta ttcaaagatt gaggggtttt tagattttat
     1981 gacagaaaaa acaatcaaag aacttgctga agaattagga gttagtaaaa cagcaattaa
     2041 caaaaaagtt tctgacgaaa atcgaaaact ttggttttcg aaaatcggaa acaagtttgt
     2101 gattaatgaa accggacaaa aggctataaa atcaatgttt tttaacgaaa acgaaaactc
     2161 aaatcaacaa ctagttggcg aaaaaacgaa aaccgaaaac cacaaaaacg ccaaccaagt
     2221 tcccgaatta gaagtccttt ctctcctaga acaacagtta aatcaaaaag atatgcaact
     2281 acaagaaaaa gacgagcaaa taaaacaaat gcaaaaatta ctcgaccacc aacaacaact
     2341 caccctgcaa gccaaccaac aaatcgagaa actgcaggaa caactacagt tgacttatac
     2401 ggaagaatct tcggaaaata aatcaacgac tctttctgaa gaaacagaat cgatagaaaa
     2461 acaatcaaag gcggagaaaa aatggtggca attctggcaa taaaaccagc aatttaaatt
     2521 aataaaaatt gctggtttta ttcttctttt tgcttttgcg agcagagcaa catggaccca
     2581 tgttgcgata ttttggtggc gccccaatcc ccgttttttc gttgaatctt actcggactg
     2641 cactcgtttt acagctgatt tttctgaatt ttgaaagagc ataaggaaac gtggtatcac
     2701 tttcttacga aaggtgctag ataggctgtg cctatcacgt ttctcagaaa aaaatttgct
     2761 atcacttttt tgccattgtg gctaggctat gtatagtgtt ttggcgaaaa atagctatac
     2821 aaatactata tttgtatggt atacataaat tatacgctga tacgaaagga aaatttccat
     2881 ggacatttta gttcgaaata tcaatcccaa aattattgca agaatagata aacgttgtca
     2941 ggaactcaca accaaaacgg gcaaaaaatg gagtcgaaat aaatatctaa aagttctaat
     3001 cgaaagtgat tttgaccgtc aacttatgaa ttacaaaaaa gatcaattcg atttattact
     3061 cgaaaaattt attgccattc aaacagaaaa tacgaacata ctgaatgact atgttcacac
     3121 aaacaaccaa ctcattgaaa cacttatcgg aggaatataa catgagaaaa acttctgttc
     3181 aaaaacacgt aaccctttat cccagtcacg ttgacgtttt agattccatc atcgaaaaaa
     3241 atccagacgt caacaattat tcagaagcca ttcgtttttt atgtatgagt tatgaacctg
     3301 ataccaatac aaccacaacc agtaaccaag caaaactcaa tgccatggga aaagaaattt
     3361 ctatcatgac agaaatcatg gcagaatttg ctgatttata tctaaaagat actggtgttc
     3421 ttatcggtac aaattctaaa gtgtatcaag aagtgaaaga aaaaattgaa aatcaactca
     3481 atccttcccg acgaaatcaa cccaaaaatc gtcaaaaaga aaccaatcaa acctttactt
     3541 ctggaaattt tgacccaaca aactttctaa aaagataaaa aaagaaaccg atccctaaaa
     3601 aaattcctcg atttcttttt agggatcagt cttccagcaa gatttgcaag ttggtaccaa
     3661 cttgcgaaaa aaaagaaaaa tccgcttatg caacttcttc tttttgtatt ttggggcgct
     3721 accccaatcc ccaagtatcg gttatttaat agcgcggaaa tccaaagaat aggacgtacc
     3781 ctgaaacaaa aaaagactgc tctcacgagc agcccaaaat gagattatcc tattcatcaa
     3841 cataaaaata ttacaaagtg aagaactaga gaaagacaaa aaaagcatat caaatcctct
     3901 caaaaataac aagtctttaa aaaatgacag ataaaaatgc acaaagaact aaaaaagaga
     3961 aatcgcgtaa aatcaagcat tagaagctag taactagcta gaccaattaa aaatcacaaa
     4021 gtgagcgtct ctatccattg gtagaggggc tacttggcat agtgtacatt atgttaacta
     4081 gctcgataaa taacgattat gttcccaaaa caatatttta tgaactataa caaaaatttg
     4141 ttatcgcata ttgtatattt taattatata attataggtg tataccactt aaatttaaca
     4201 caaag
//
LOCUS       DANJMI010000117         4067 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000117 DANJMI010000000
VERSION     DANJMI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.123"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(<1..1157)
                     /locus_tag="PWU81_002994"
     CDS             complement(<1..1157)
                     /locus_tag="PWU81_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1111607.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
     gene            complement(1160..3409)
                     /locus_tag="PWU81_002995"
     CDS             complement(1160..3409)
                     /locus_tag="PWU81_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL1111608.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            complement(3506..3961)
                     /locus_tag="PWU81_002996"
                     /pseudo
     CDS             complement(3506..3961)
                     /locus_tag="PWU81_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         1311 a          744 c          580 g         1432 t
ORIGIN      
        1 actctagtat tttttttatt tgtcgcaaaa aatgttacaa ataggatcac aataaatatt
       61 cctgcgaata caaaaaatgg agcagtcaaa tttgtacttc ttgttagaaa ttgtgctaat
      121 tgcatagcaa taggagctat aaaattacca atattacagc caataaaaat caatgaagta
      181 gcaaaattaa ttgtattctt tgtagtgact ttatctaagt tactaaagat aaatgggaag
      241 caccatgatc ctgttatact tgataaaaat aaacccgtta gcaggaacgt catattaccg
      301 tttgacacag caattaacaa atttgaaatt ataaagaata taattcccaa gtatagggtt
      361 ccttttccta tccacttatt tattggtcca aacataaatc ctgctagtat tcctaagata
      421 ggcattagag ctaaatagtt actagcatta aagtttaccc ctttaatttc agttgcaatt
      481 gatgcaaatc gtacagaaat agcaatacta ttcattacga gtaagattgc aaatagaact
      541 agggcaaaaa ccattggatt catcttttct tttacatgat tttcaggtat gtcactattt
      601 tcatctctaa tttcaggaac ttttagcgcg aataagagag ctacaggaaa cgcaattgca
      661 tagactaaaa acgaagccgt ccaattaatg ttcactaaaa ctccagcaag aaaaataagg
      721 attgtctgac ctaaagcttc cattgaattt ctaataccta atagcgttgc tctagtattg
      781 ccagagtata aagagctgat atatgtcaca gccaatgaat tatatactcc taaaccggca
      841 cctaatatta ctcgactaat aagaacggtt tggaaggtag ctgtaaatac tggaataatt
      901 cctcctatac cagctaataa taaacctaaa attattgtct ttttcattcc tattcttttg
      961 gcaaagtaac tagataataa gataaacaaa ataacagtta tacttggtgc agttcctaaa
     1021 agttcacttt gactttgtga tatccccaaa gactgtttca tttgtggaat aacaccatta
     1081 attgcttgag agcttgttaa cattagtgaa atacttaaga ttgctccttt ttgaagtaag
     1141 cttttttggt tattcatctc tacttttcct cctgtgttat aacccaaatc ttggaatcaa
     1201 aatcaaatgt ttctgggcgt cgattctgat catttatttg tccactagat gcatctgaac
     1261 agactagtcc tgctctcatc aattccattc cagatcgcaa actattttgt cctacattat
     1321 aaagtccgtc attatctaac ccttttaatt tcagtcggcg gtaaggagca tttacctcat
     1381 ttaatgtttt ataatgagca acaatagccg ttttcttatc atgactaaca accatccacg
     1441 cggtttgatt atcatcatcg aatggtgata atagtctata aaaagtacca ttatgaatta
     1501 tggttctata ttttttataa aaggcaattt gttcttttac aatcctcaaa tcgccttcac
     1561 ttaattcatt taagtcaagc tcatatccaa atgcaccaaa aaaagctaca tttcctctag
     1621 tttttatagg tgttatacgg tttgtttgat gatttggaac aattgataca tgagcaccaa
     1681 tagacgaaag aggatacagc atactagttc catattgaat ttttaacctt tcaattgcat
     1741 ctgaatcatc actagcccaa gcttgaggtg catagtgaag cattccagga tcaaagcgcc
     1801 caccacctga tgaacaagac tcaaatagta tgttaggata ttttgttata agtttttcat
     1861 ataagcgata tacgcctagt acatatctgt ggaatacttc tccttgttga tctgctgaca
     1921 agtgactgtc aaatacatca gtcaatggac gattcatatc ccacttaatg taatctaagt
     1981 ttgattcatc aatgatttta cacatttgat cgaaaatatt ttcaacaacc tcatctaacc
     2041 caaaatttaa tacatattgg ttcctaccat gattaggttt tcgattaggt gtagaaataa
     2101 tccaattcgg atgtgtgcga tataactcac tatccttatt caccatttca ggttcaaacc
     2161 ataaaccaaa tttcatacca aaactacgaa ttttttctga aaggctgccg attcctctag
     2221 gtagtctttt aggatttaca tgccaatctc ctaatcctgc acaatcgttt tctctttctc
     2281 caaaccagcc atcatctaat acaaataatt caataccaac atcaaatgct tttttagcaa
     2341 tatttaccaa cttttcttcg tcaaaatcaa aatatgttgc ttcccaatta ttaattaaga
     2401 caggacggtc tttttctcgc cacttaccac gagctaagcg ttttctaaat agttgatgaa
     2461 aagtttgact catggcattt aaacccttac ttgaataaac cagaatcgct tctggtgctg
     2521 taaatatttc tcctggattg agtttccaat taaatccaaa tggattgatg ccaacttgta
     2581 gtctagtaac atcccatgta tctacttctg cttgaattaa gaagtttcct gagtaaacta
     2641 gtgctgcacc aatagcttct ccttgatttt catcagtatt ttttcttttt aaagtaacaa
     2701 atgggttatg ctgatggctt gaaatacctc gagtagattc gattgattga attccttgtt
     2761 gtaaagtacg cgacttaata gttctttctc taccccaagc accagataat tgttgccatt
     2821 cataatttgc atcgggtaaa tccaatgata aactgctaat attctcgatt atttgaatat
     2881 cttttccttt attcataact cgcgcgctac gagtaataac aggataatct ctataaattg
     2941 tatataataa ttctacttga acatttgtaa ctgaatctaa aatataaact ttcaatgttt
     3001 tgctttcatc attattatca acataggtag caggtaaacc ttctagttta ggttttcctt
     3061 cttccacttc gtacccttga tagacaaaat ctgataagcg agagccattt ttctgtagta
     3121 atgaaatagc aggatgtcta aaatctgttg taccatattc aggaaattct tgttttaagt
     3181 gctctaacga atatagcaaa tcgttttcat ttctataggt tgtcataggc cgatgatgca
     3241 tctcaattag atgactatag tcattatctg gtaagttctc accataatat aaatgcaata
     3301 atttaccatc ttccgatatc ttaaaaatgt aacttatctc ctgattaaac aaatgaaatt
     3361 gttggttttc tgatcgataa ataatattca tttcattatc cttatacata agctctcctc
     3421 cgtttcttta ttgcattata aaaaacgctc acataaatag taagcgttta aagtaacgaa
     3481 tatttatcaa aatcaaatgt ttagtttatt caccattaat gattcctaga cctaatctac
     3541 gttttcgata tactcccggt gaaacaccat ttgctttctt aaaggcttta gaaaaagatt
     3601 gtactgttga gaatcctatc ttgtcactga tttgctggat tgaatattca gttgatgata
     3661 acagatcgtt tgcaatagaa attttgattt cactaatata ctcttttatt gatattccca
     3721 tatcattctt aaacaatcgt gataaataat tttcgtgtat cgatagactg acagcaatat
     3781 ctttaacaga taatttttga tacgcattct cacgtattat ttcaattgtt tgacgaacaa
     3841 aaggattcat ctttttagta attatccctt cgttcagata actagctgaa tttaagagga
     3901 gttctaaaaa tttataaact tgtttttgtc tggacaattc actgactaga ttgtcctgat
     3961 ctttcagctg agtgttaggt tctgttacta aatatataga attttccata tccaccctcc
     4021 tatgttagat ttggataatt tttgttttat tatgtttata ttattac
//
LOCUS       DANJMI010000118         4066 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000118 DANJMI010000000
VERSION     DANJMI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.124"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            85..1434
                     /locus_tag="PWU81_002997"
     CDS             85..1434
                     /locus_tag="PWU81_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL1111609.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="PWU81_002998"
     CDS             complement(1562..2152)
                     /locus_tag="PWU81_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL1111610.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="PWU81_002999"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="PWU81_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL1111611.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1172 a          744 c          789 g         1361 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     4021 tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DANJMI010000119         4030 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000119 DANJMI010000000
VERSION     DANJMI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.125"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            958..1926
                     /locus_tag="PWU81_003000"
     CDS             958..1926
                     /locus_tag="PWU81_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL1111612.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            1936..2424
                     /locus_tag="PWU81_003001"
     CDS             1936..2424
                     /locus_tag="PWU81_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL1111613.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            2720..3001
                     /locus_tag="PWU81_003002"
     CDS             2720..3001
                     /locus_tag="PWU81_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111614.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            3003..3419
                     /locus_tag="PWU81_003003"
     CDS             3003..3419
                     /locus_tag="PWU81_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111615.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT         1502 a          702 c          671 g         1155 t
ORIGIN      
        1 aggtatttgg ctagcaaata tagagcttgc caagcccttt agtgtgtttt gtagaaaggg
       61 accttatagt cactttcaca ttacaaacga tacgtccata ggacgtatca aaagggtggg
      121 acttatgagc aaattaaagc aaaatttggc tagcaatcct tctaaaaaat acacctattg
      181 atcagagtca aaataaatca gttttcgagt gagcgaatcc aaatttgcaa ctctgaagct
      241 ttgcaaatga gtgtgccggc ttccgtaaaa gtctaggtaa cattagtcga tatatacata
      301 gggctctaat ttgcgtttta aggcgtttta aacgttctgg tatgtaatta taccaaagcg
      361 actttaaaac gccttaaaac gaattttaga ggttctgtag aacgttttag ggttgttagg
      421 gcttgccctg accgcctgta aggcgcttta aaaacaagat aagggatttt atctagactt
      481 gtcttaaaac gccgtagatg agcgctcaga gcttctaaaa ggactcctta cgaactgttt
      541 ttaagcaaag gagcctttag gcgactgcgc agtttaatgt gagagcgtag cgatcacacc
      601 tttaaagcga ccgtagggag cagattccca gtttaggaaa tttcgcgaac ctgtttggag
      661 aaaaaaagta ggaaaatttt aggcgggaaa ttttgccccc ctcttttata atctctttta
      721 taacctcttt ttaaacctct ttaccgtcgg ctgtatcctt actctcccaa ggtgtgcaga
      781 atcttttagg ggtctttttg tctgttttta ggggtctttt tgtctgtttt taggggtctt
      841 tttgtctgtt tttaggggtc tttttgtctg tttttagggg tctttaatta gggggttgac
      901 ccccattaga aagctcttta taattttgtt aagtagttaa tcttgaagga ggattttatg
      961 gcaaatgaaa ttgtgaaata ccaccatgaa ttaaatacga ttcctttacg caaatttacg
     1021 tcagtagaaa tgaatttatt cttctctata atatctagaa tgcgtgatgt aggcgataaa
     1081 aaagtacaat ttacattcga acaattaaaa gatttaagca attataaagc aactgcaaat
     1141 gttcgattca tagatgattt agaaacaacg tatgacaaat taatggattt acgttttggt
     1201 agaagaagtg ctgacggttt gcaacgtgaa cgatttgttt tatttaatca atttaaaatc
     1261 gatggaaaag ctgatattcc ttttgctgaa atacaagtcc atgagaaagc cttaccctta
     1321 cttaataatc ttgaagaatg ggttcgatat tctctccaac aattcaatga attagaaagc
     1381 agttattcca aaaccatgtt tagattatta aaacaataca gaaccaaagg aattgccaat
     1441 ttttcaaaag aagactttca tgaattactt gatatcccta aaagttatag agaaagtgat
     1501 ataaataaaa aaattataaa gcctattcgc caagaactaa ccgcaatctt caaaggtttg
     1561 acaatccaaa agagatacgg aaaaggacgg ggaaagcctg tcattggcta tcaatttaca
     1621 tttaaacctg agataaaaaa atcagacgac tttaacaaac atgctccaac caaagaagaa
     1681 caaaataaag ctttggaact agctaatcaa caggttcaat ctgaaaaaga acctacttca
     1741 ttacaagaaa aaatcaaaga acaaaaacga aaattagaac ttttacaaca gttagcagaa
     1801 ttagaaagaa aacaaaatac cccaagtaag aaaattaaca aacaaatagc agataacaat
     1861 tccgatatta ccccagaatt taaagaaaac ttactagata atctccaaaa cttatttaaa
     1921 gaataagggg tttttatgac agataaaact atcaaagaat tagcagatat tctaggtgtt
     1981 tcaaaagata aagtgaagta tcaactgagg aaattaccta gtaattttac ctacaaaaaa
     2041 ggaaatatta cctacctgaa aaaagaagca gtaacaacaa ttacaaaggt tttatcaggt
     2101 aatgaatcgg gtaacttacc tagtgaatta cccgattcat tacctaccga ttacctactc
     2161 aaggagattg aaactaagaa taatcatatt gaagaaaaag ataggcaaat tgaaaaactt
     2221 caaatagctt tagaaacgca gcaaaaatta ctcgaccagc aacaacaact caccctacaa
     2281 gccaatcaac aaatcaaaaa acttcacgaa caactccagc taacctatgc agaagaatct
     2341 tcagaaaaag aacccctttc tgttactgaa gaaactcaaa aaggacaaaa caaggagaaa
     2401 aaatggtggc aattctggca ataacccaac aatttttatt cataaaaatt gttgggttac
     2461 tttctttttt tgcttttaca agcatagcga catgatctca cgtcgctatt ttttaaagag
     2521 cataaggaaa cgcggtatca ctttcttacg aaaggtgcta ggtaggctgt gcctaccacg
     2581 tttctaagaa aaaaatttgc tatcactttt tcgtcattgt ggatagctta tgtatagtgt
     2641 ttcggcaaaa aacagctata caaatactat atttgtatga tatacataaa ttatacacaa
     2701 atgcgaaagg aattcttcta tggacatttt agttcggaat attgatccaa aagttatttc
     2761 agaaatagat agacgttgcc aagaactcac aacaaaaact ggcaaaaaat ggagccgtaa
     2821 tgcgtaccta aaagttctaa tcgaaaacga ttttgaccag gcacttaggc attacaaaaa
     2881 agatcaattc gatttattac tcgaaaaatt tatcgatata caagcaaaaa atactgaaac
     2941 attgaacgac tatgttcata caaacaacca actcattgaa acacttatcg gaggaatata
     3001 acatgagaaa aacttctgtt caaaaacacg ttacccttta tcccagtcac attgacgttt
     3061 tagattccat catcgaaaaa aatccagata tcaataacta ctcggaagcc attcgttttc
     3121 tatgtatgag ttatgaacct aatactaata cgacaactac cagcaaccaa gcaaaactaa
     3181 atgcgatggg aaaagaaatt tctattatgt ccgaaatcat tgcagaattt gctgatttat
     3241 atctaaaaga tactggtgtt cttatcggta caaattctaa agtatatcaa gaagtgaaag
     3301 aaaaaattga aaatcaactc aatccctccc gacgaaatca acccaaaaat cgtcaaaaag
     3361 aaaccaatca aacattcact tctagaaatt ttgacccaac aaactttcta aaaagatgaa
     3421 aaagaaaccg aaaatttttt ctatttctca gtttcttttt tagggggcag tcttccagca
     3481 agatttgcaa gttaccggta acttgcacaa aaaaagaaaa atccgcttgt gcgaatcctt
     3541 ctttttgcat tttggggcgc tgccccaatc tccaaatatc ggttatttaa tagcgtggta
     3601 actcaaaaaa atagaacgta ctctgaaata aaaaaagact gctctaacga gcagccaata
     3661 aaaaataatg agcgtttatt cttgaaaaag cagcgcgctt atgaagcgct gccaaaaatt
     3721 taccatattt tatgaaaaat aacaagtttc taaaaaggaa tcggttaaga ttcacaaaga
     3781 atcgtaaaca gacaatagcg gaaaatcaag cttccgaacg taccttttag ctagaccaat
     3841 gaaaaatcac aaagtgagcg gctggatccc ttggtacagg cgttacttgg tagtatacac
     3901 attatgttaa ctaacgctat aaataacgat tatgttccca aaacaatatt ttatgaacta
     3961 taacaaaaat ttgttatcgc atattgtata ttttaattat aggtgtatac cacttaaatt
     4021 taacacaaag
//
LOCUS       DANJMI010000120         3878 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000120 DANJMI010000000
VERSION     DANJMI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.126"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(718..3867)
                     /locus_tag="PWU81_003004"
     CDS             complement(718..3867)
                     /locus_tag="PWU81_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL1111616.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          637 g         1362 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttggaca cttgaatctc ggctgttttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcttccttc acgaatgggc tgattaggaa actcttttgt
     2041 taaccagtca ggttctttcg tcatttcttt gatcgctcga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa aatcaaattt
     2161 tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gtagggtcca aataactttc ttttttgaca ggctccattt gatcaatcat
     2281 acgattgatc tgttccggtg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctaatgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 aaccgagcca tcttctaatg cattcttgat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtc gtacgtgcca attgcccttt ggcttgtttc cgattctcag ggaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt gccccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttt tttgcatatt tcaacgccgc
     2701 atctagcttt tgacgggttg taataatcac aacatttgca ttggcatctg ccaacagagt
     2761 ttcacttaac tctttggcac ttcctgttcc cacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaatttcgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgtccgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ttttcctgaa ccagtcgcat gccacacata
     3001 tcctgattga tgtttatttg cagcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgaggattt tattgtcttg atcttcactg acaatcgtat aatccgcaat
     3121 caagcgatga gcatccggga tatttagcac ttgtttgcaa aactcatata agttttctac
     3181 ctttttatta tctttcgttc gccaactaaa taaaaactta ggatgcaaat cctttggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaacgcctg
     3361 ataaaaacta tcttttgcgg ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gatcagtaag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aggtagagat tcctccacca atgtcttgat tagaataaag
     3541 aatgagcgag acagaaccca gttgcggatc ttcccgttca atcgtgatcc gagccattcc
     3601 attttcacct tttaaccatt tcgcagcatc aaagggggtt ttcgttcgta gtaacaattc
     3661 tgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagatg attttttggc gtaaattgac ccaaagatct tcttctgatt ttagatcggg
     3781 acgatacgtc cactgattat gcccttcccc caatacacgg atcaactgtt cttcgacttc
     3841 cgcttcctca cggtgactaa ttttcattta tcttccct
//
LOCUS       DANJMI010000121         3718 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000121 DANJMI010000000
VERSION     DANJMI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.128"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(<1..353)
                     /locus_tag="PWU81_003005"
     CDS             complement(<1..353)
                     /locus_tag="PWU81_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111617.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRT"
     gene            complement(613..1161)
                     /locus_tag="PWU81_003006"
     CDS             complement(613..1161)
                     /locus_tag="PWU81_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111618.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="PWU81_003007"
     CDS             complement(1162..2019)
                     /locus_tag="PWU81_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL1111619.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="PWU81_003008"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="PWU81_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="PWU81_003009"
     CDS             complement(2581..2910)
                     /locus_tag="PWU81_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL1111620.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3259)
                     /locus_tag="PWU81_003010"
     CDS             complement(2987..3259)
                     /locus_tag="PWU81_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL1111621.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3259..3522)
                     /locus_tag="PWU81_003011"
     CDS             complement(3259..3522)
                     /locus_tag="PWU81_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL1111622.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1091 a          754 c          473 g         1400 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac tctatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttataaacaa
     3061 cccggtgttg tctattcaat cttctagaat acctaccttc atgtgtaggt tttaacttct
     3121 caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc ttctcaaatt
     3181 gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag tttttaatca
     3241 tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat tagtaaatcc
     3301 actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct caagtaatag
     3361 cgtttctttg attgctttcc aatcatccaa accaataagc accgcattat tttcactgcg
     3421 ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca acttataaaa
     3481 atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc cccctctaat
     3541 ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt tgtttaaatt
     3601 ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatacccgt ggtgctagtt
     3661 aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa tcaacaaaaa aacgcata
//
LOCUS       DANJMI010000122         3717 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000122 DANJMI010000000
VERSION     DANJMI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.129"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            72..155
                     /locus_tag="PWU81_003012"
     CDS             72..155
                     /locus_tag="PWU81_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HDL1111623.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            280..1017
                     /gene="erm(B)"
                     /locus_tag="PWU81_003013"
     CDS             280..1017
                     /gene="erm(B)"
                     /locus_tag="PWU81_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL1111624.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1280..2143)
                     /locus_tag="PWU81_003014"
     CDS             complement(1280..2143)
                     /locus_tag="PWU81_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL1111625.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2145..2417)
                     /locus_tag="PWU81_003015"
     CDS             complement(2145..2417)
                     /locus_tag="PWU81_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL1111626.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2434..2643)
                     /locus_tag="PWU81_003016"
     CDS             complement(2434..2643)
                     /locus_tag="PWU81_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDL1111627.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2721..3617)
                     /locus_tag="PWU81_003017"
     CDS             complement(2721..3617)
                     /locus_tag="PWU81_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL1111628.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1143 a          652 c          575 g         1347 t
ORIGIN      
        1 aaattttgta taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg
       61 agggattcgt catgttggta ttccaaatgc gtaatgtaga taaaacatct accgttttga
      121 aacagactaa aaacagtgat tacgcagata aataaatacg ttagattaat tcctaccagt
      181 gactaatctt atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct
      241 gtatttattt atagatgtaa tcacttcagg agtgattaca tgaacaaaaa tataaaatat
      301 tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta
      361 aaagaaaccg ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg
      421 gctaaaataa gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg
      481 tcagaaaaat taaaactgaa cactcgtgtc actttaattc accaagatat tctacagttt
      541 caattcccta acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca
      601 caaattatta aaaaagtggt ttttgaaagc catgcgtctg acatctatct gattgttgaa
      661 gaaggattct acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact
      721 caagtctcga ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa
      781 gtaaacagtg tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg
      841 aagctatata cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact
      901 aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccgttact
      961 tatgagcaag tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc
     1021 tatgagtcgc ttttgtaaat ttggaaagtt acacgttact aaagggaatg tagataaatt
     1081 attaggtata gggcacctct aataactacc aattaattca cctcaaaatg aaattagaaa
     1141 aaaacagcga aatttttaaa tctatttctt atcgatacaa attcctcgta ggcgctcggg
     1201 accccaacat gaggaaatgt ttttataaaa aaagagtagt taccgttttt tggtaactac
     1261 tcttttcctt ttttaaggtt taaatacctg gaagtttagg tgttttgggt attggtggtg
     1321 taggtagctg caagctttcc attttttgtt gaattgcttt aaattcaggg gtttcttggt
     1381 gttgattttg aaccattttt tgctctattc tctccaaagt cggttgaatt tctttccctg
     1441 atactttacg atttaattct ctttctaagg tctctttcgg actaatggaa ggcgtttcta
     1501 agcttgaata gagtttcact ccttctctat tgtaaagcct tatatcgcta aataagcccg
     1561 ttttatgaag ggtttctaaa ttggtcggta agtttttgac aacaatatca tgcgcttgtt
     1621 ttggtgttgc cctagctgtc attggatcat ctgcatacat ggtttcatat cgttcaatcg
     1681 ttcctaagta tgattcaatt ttcggtactg ccatgacgta tatttttgtt tcataacctt
     1741 tagattgaag cattgtggct gtttgaatgg gaacgtctgt tgttcgtcct gttccttcaa
     1801 tcactaaatt atagccttga tcgcttaaac ggctaataag tgcttctgtc atgcgattcg
     1861 aataaggggt aacgtgtttt actacgtctt tttcatagag tttcactaat tcgtcaaaat
     1921 ttgggtgctg ttgtttaaag gtatcattat caatgacaat aacattccct tgtgtttctt
     1981 caaaaattgc tgatcgcaaa ctggttttcc ctgaccctgg ttgcccacca agtaaaaaag
     2041 cggttggcga ttcaaccgct agtctatttt tagttagtcg ttcaacattc ttttctaaac
     2101 gatcttcaaa ttgcttttcg gtgaagtcag taatattcgc catgttaagc cactttctct
     2161 ttattcaaaa tttgttttga atattgattc attgaccgcc aatactcatg aatagcttgt
     2221 aattcttcaa atggttgttg aaataaatca acttcttgtg ccacttctaa ttgatttaaa
     2281 agattcactt ctagtaaacg agtattttta gtatctagtt catgattcaa aacataattt
     2341 aatacccgat tataaacact tgccgataac tcattaatga tttcaatttc aaatgttttt
     2401 tcatacgtaa ctgccatttc attttcactc cttttaaagt ttgtcaggta aatatttccg
     2461 tatatattct tctaaggctt gatccataac ttcttttacg tttccgccat tctttgctgt
     2521 ttcgattttt atgatatggt gcaagtcggc acgaacacga accgtcttat cccccattat
     2581 atcttttttt gcgctgattg gcgtatcatt tcgttttgct ttctgtgcgc ctaaatttcc
     2641 cacgatcgct cacttctttc tatttcttct tactatttat tttatcatca cctatcacaa
     2701 atcacaagtg atttgtgatt ctattctttt tcattttcta attgaatgat tcgctcaatc
     2761 atttcaaaaa atacgttctc atacatagac aaaacttttt tgtcatagcc tttgtgttct
     2821 gtaatgccat ttttagacca agtacttact ttattacttc gcttgataat atttcggaaa
     2881 accaaattat cctctttatg ttctttgtac agttcttcca ggtttgattt tatcgttgcg
     2941 ctgtccgtat caactaaata aggaacaaaa ccaatcatat ctagtccagg gttaaactgt
     3001 tcttgcaaat caatcaaata ggaaatatag ttttgaatgt tgtttgtact ttcttcttct
     3061 gcttgtaaag ggatcataac gtaatcactc gccacgattg cattatttgt ataaacgctt
     3121 ggcgttggta cagtatcaat aataataaga tcatagtcac tttttaaagg tgctaaaaga
     3181 gtagcaagca atctactttc attttcaaac gtccatgagc gagttaattt tggcagtaac
     3241 atcaaatcaa acgtgccagg gatcaagtct aaattatcag tcaaatgaat aatagaagaa
     3301 gccaagtttc catttttcaa gccttcataa aaattgacac gtggcaattc tacctcaaaa
     3361 gtttttgcta agtcttttgt caatgttgcc tgtaagtcct tatcgatcat taaaactttt
     3421 aaattcaatt tgtctgtcaa gtaagcaaac atagtcgata atttggactt tccaacacca
     3481 cctttaaagt aattattcaa gataataatt gcttcatttt tattgtttaa aatacgtctt
     3541 aattcttcaa gtatttttag tttttccttc tccataacta cgccccattc tttttttgta
     3601 tagtaatatt gtatcattaa gaatcacaaa tcacaagtga tttgtgatta atcacttgtg
     3661 atttgtgata agtgatttgt gattaatata taaaagccct ctttaaaggg cttttat
//
LOCUS       DANJMI010000123         3677 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000123 DANJMI010000000
VERSION     DANJMI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.130"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            64..972
                     /locus_tag="PWU81_003018"
     CDS             64..972
                     /locus_tag="PWU81_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDL1111629.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1592
                     /locus_tag="PWU81_003019"
     CDS             972..1592
                     /locus_tag="PWU81_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111630.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1596..2045
                     /locus_tag="PWU81_003020"
     CDS             1596..2045
                     /locus_tag="PWU81_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111631.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            2045..2539
                     /locus_tag="PWU81_003021"
     CDS             2045..2539
                     /locus_tag="PWU81_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111632.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            2553..2984
                     /locus_tag="PWU81_003022"
     CDS             2553..2984
                     /locus_tag="PWU81_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111633.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            2986..3123
                     /locus_tag="PWU81_003023"
     CDS             2986..3123
                     /locus_tag="PWU81_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL1111634.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            3160..3453
                     /locus_tag="PWU81_003024"
     CDS             3160..3453
                     /locus_tag="PWU81_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111635.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            3450..3674
                     /locus_tag="PWU81_003025"
     CDS             3450..3674
                     /locus_tag="PWU81_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL1111636.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT         1324 a          546 c          814 g          993 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgtcaatcag tcatatgtag
     1501 atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa agaattccaa
     1561 attttacttt tgaaaaaatt ggggaggtat agagaatgac agatatcgta aaaataaaac
     1621 aaagcaatgt acaggtttat ccacaaactc attggaatgc tatagaaggc aaaccaacaa
     1681 cggtgaaggg agataaaggc gatcctggcc aagcagcaac aattaccata ggaaccgttt
     1741 ctagtggttc aaccgcttct gttacgaatg taggaacatc atctgcagct agatttaatt
     1801 ttgttttacc aaagggagat aaaggtgatc ctggaataaa tgccacaact acagctgtag
     1861 ctacaactac agcaaatggg ttgatgtcgt ccacagataa aactaagttg gatggaattg
     1921 cagctggagc acagaaaaat ccaggtaatg ctacaactac gacagcaggt ttaatgtcag
     1981 caaccgataa agtgaaactt gatggattag cgaatattac atttgagaaa gtggggacgg
     2041 tttaatggct gatattgttc aattaaaaga agacggagtt gctaaatact taaaaacgca
     2101 tgtagaagct attgatggta aagaagcatt ggtgcagaca gatggagacc aagccattgc
     2161 aggacataag aatttttcag gttctgtaac aatcaataat aaacgtgttt tgacgactga
     2221 tgataacaga tatgaagtgg taaatctggt tgtcactaac ggtaatacag ggacagcaaa
     2281 gctttatcgt gaaggaaaaa cagtttctat atattttgtg gctttaaacg gaaaaagtag
     2341 tggcgggaat gattcggtta ttttaactgt tccagaaggc tatcggacac caattagttt
     2401 tgaacaactg gttgggtcaa tagaccgttc tactttgaac agtgctcaac tatctattgg
     2461 cgcagacgga gccattaaat ggagaagaaa ttcaagttat ggatcagctt attcatttgt
     2521 tatcacttat tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc
     2581 cttacggatt cgagcaaatc attttgaaca atcaagaaaa tatccctgaa aactgtacag
     2641 agattaaacc accagttcct aactggagac caagatttga ttttgataaa aaacagtggg
     2701 ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt tgatagcgta gatgaattgg
     2761 ctaatattaa ggcattatac gaaacgctga aggcagaaaa tgatgaattg aaacagctaa
     2821 attctaaagc aatgctaaac aatgtagcaa tcaagcaaga aaatgtctta ttgaaagaaa
     2881 agtcagaaag tttagcgcag ttgaattcaa aaacgatgct tgcttctgta caaaatacca
     2941 aggaaatcga agaaatcaaa aaacaattac aaggtgggaa gtaacatgta ttcatatgaa
     3001 gatatcaaac taatgtatga ctggggctgt ttcactaacg aacaagtaat ggttttcgtt
     3061 ccattatgca ttacagacga agaagcagat aaaatcatta ataaagataa gagcgcatct
     3121 taattgatgc gtttttattt aaggtaaagg agttgtcaca tgattaattt aggggaatgg
     3181 ggaatgatag caggatcaat aaccgctatc gtttctttga ttttattagt aattagaccg
     3241 attgctgcat ctttctcgaa aattactgag actctttcaa aagtaagcca caatttagat
     3301 ttgctgacta aagatttaga atcgagcaaa tcagatcgat tgatgattca tgaagaacta
     3361 aagaaacacg atgaaagatt agatacacat acagaaaaat tggtggaaca cacgcaacaa
     3421 atcaaaactt tgtttaggga gagaagaaaa tgaataataa aacgttcgaa gtactaaaat
     3481 ggttcgcact ggtaattatt cccgcactag ctactttcgt ggggttagtt ggtaaagcgc
     3541 tcaattggca gtacacagat atctgtgttg tcatcattac tggttttggc gcgtttttag
     3601 ggagtgtgtt gggtgtatca aatcgaacct acaaaatgtt ctcggctgaa agcgaagaag
     3661 gaggaaacaa atgaaaa
//
LOCUS       DANJMI010000124         3489 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000124 DANJMI010000000
VERSION     DANJMI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.131"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(<1..1838)
                     /locus_tag="PWU81_003026"
     CDS             complement(<1..1838)
                     /locus_tag="PWU81_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1111637.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
     gene            complement(1835..2575)
                     /locus_tag="PWU81_003027"
     CDS             complement(1835..2575)
                     /locus_tag="PWU81_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL1111638.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            complement(2565..>3489)
                     /locus_tag="PWU81_003028"
     CDS             complement(2565..>3489)
                     /locus_tag="PWU81_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL1111639.1"
                     /translation="NIWTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAI
                     QKWIELKFSIIQTWIDLKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTW
                     NNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDG
                     LINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDK
                     EQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDS
                     MVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
BASE COUNT         1096 a          665 c          417 g         1311 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcac taggataccc accagtagat
       61 tctccacctc ccgatggacc accaccttgt ccaggaatta tttcttttcc tttaactgta
      121 agttttcctt gtacatctaa gtctccaaaa taaattgctt taccatttcc taataaaact
      181 aatccttttc ctactttagg agaaatcaaa atatatttgc cgtctccatt tgtacgaata
      241 actaaagaat tatcttcaat aggggttgga gtagaagccc caggaaaagg attaccaaca
      301 gaatcagttg ttccaatcgt tccaattgaa cccttagaat tccaaaattc cattcctttt
      361 ttagttaact ccattatttt ctttttgttg ttccaaattt ggagtagtcc attaactaat
      421 ttcaatacat ctccagtctt gttaaaagaa ttttgaaaga tatctgcttt gattaatcca
      481 gtttgtataa aattggcatt aaatatacta tctaatgtcc aagctgaatt aaaaggcccc
      541 cgccatcctt tcttagaaaa agcaatccca tttttattca ttcttagtac ttctcttgcc
      601 ttttctaaat ctggattatc cataataaag atgttggaag gtttttcttt tggccataaa
      661 actacatatc ctcctgcact gccttgacct gtaatcattg aagaaacata atcattaaaa
      721 tcactcatat aattattcgt tgcataatct tttaatttat cctgaatagt tacagcttgt
      781 tgctgataaa aagcaacttg aatgtctcct gcttctaatt tcaaagtttt ttcagataaa
      841 gaatcgtatt gtagtccact aacctttgat tcaagataaa tattatattt tttatgatat
      901 attttaaatg tatcaaacag accataattt ctaatcttcg caaattcttt cgcttcttca
      961 ctatcagtca acttatcaaa ttcaaccgta atagaaactt taggcttatc acagccagga
     1021 ttgatcgttt taaagtaatt tttagctatt ttatttaagc tcttaatgtc ttttactcct
     1081 tgttcttctg taaactgaac atgttctgta tagacatcag gatagttatt aatataggca
     1141 ctatctactg gcgaaccata aattcgatta gtcgtaccta cttcactttg aggatcagca
     1201 tatggaataa ttcttgtttt tatacctgtc caatctaatt tgaccttcaa accagacata
     1261 tcttttccat accgaatcgt tccaacatta ttgcgacctc ttcgctttaa caaagaaagc
     1321 ttaaatggtt cacgtttaat ttctccgccc caatattgaa gcaatgatcc ttgttcacca
     1381 gcaatacaat ttaaaacatt tctcgcttca aagattgtgc tagaaacagc cgtaatatca
     1441 gaataaagtc gtatgtcaga ttttttatcc attttagttt caataattga tatcgcttct
     1501 tgaccagttt tagagtccac ttccacaaga gtgactaccc gtcttccaag tcgatttgta
     1561 cgactctgag cataaatagt tactgtattt aaaaaagtat ctatatcttt atcatcaata
     1621 aaaaagatat gatactcttc ttgatcgttt ggctttgctt ttatttgata gtcattttca
     1681 aaatattcat caaatctagt tcctaaagga taatccaact ctaattcata ttttccatta
     1741 gctacttcat atatttcaca tcttgtacaa tcctttaaaa ttcctaagcc attcgttgag
     1801 aaatctgttt cagtaggact atatattctt ggtttcatac ttttctccac cacctaggca
     1861 ttatttcaaa tgaatgaata tttttcgtcc atttaatttc attttttcca ggatataaaa
     1921 atggaaaatc taaaaataag gtgacctgat cctgatgttc taaattacca tctaattttc
     1981 gataagcctc ttctaattta gaatctataa ataattctct gtccaacgat ttcaaatcat
     2041 atttatcctt attaatataa aaagaagcat ctccagaacc actcaattta ataagcggtt
     2101 ttgaagaata cttctctggg ttataaattt caaaagtttc tgtttgtcga atagtaaatc
     2161 taccattgta attttcctta aatggtcgaa tactcactgt aaattcaaaa gggacaatat
     2221 ttcctgtttt tcttgttcct ttaaattcag gtccttctat tactacagct tgataaatat
     2281 attgttcatc atagtaaaga ataaaatcac tataagacga catatctaac cattcagtga
     2341 ttcgatcttc ccactcctgc accatatcaa ttgaaggtgc tttatagtaa cactcaatct
     2401 ttctagtcac atttttgtaa tagcctttat caataatgat agaatcattt ccttctcgct
     2461 ctctcaattc aattactcga ctagctgaaa cagaagcagg cctgttttga atatacacat
     2521 taaattcaga agaataatgt tgattaataa aaaattgtcc tctcttaagt tgcatataat
     2581 gttcctccta ctgcaccagc atctcgtttc atttgtcttg tcaattctgt tttaattttt
     2641 gtagtaatct ttcgaaccat tgaatcaggt aaatctccat aaacatttaa atgtaaatga
     2701 atttcttttg tttcactagc agatatattt tgctttgttt gtgtaattgg taggtgactt
     2761 attccattta ctacaggttg aacagacggt gtttttacta aatttgtcat ggtttgatct
     2821 aacttacttt gctctttatc aataccaacg ataatacctt gaacaatatt tttaccaacc
     2881 atatcacgca tccatcttga tggagaatga atatctaaag ctcctttaat ccaatttttg
     2941 atattacctg caataccttt gatagtatct ttcaaagcat tccacttttc ttttacacca
     3001 tttataagtc catcaatgat gttcttgcca atttcaaata aattgacttc ccttaacgaa
     3061 ttaaagattt cttttacccg attaatagca tttgaaacac cattttttag atttgtccat
     3121 gcattttcag cagaattaac aattccagta acaatattcc agaaagagtc cttaatgtta
     3181 ttccatgtat taatcatggt attttttata gaaatccacg tattgtatgc cgtatcttta
     3241 atatttttcc aagtatcttt gaaaaactgt ttaatattat tccaagtagt aatggcatta
     3301 tattttaaat cgatccaagt ttgaataata gaaaacttca attcaatcca tttctgaatt
     3361 gcaaaatatt ttatatcaat ccaaagatta ataaaaaaat actttatgtt taaccaaatc
     3421 gatttagctt gatttaccac ttcattccaa atatttatca atgtaagctt gaaccctgtc
     3481 caaatattg
//
LOCUS       DANJMI010000125         3162 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000125 DANJMI010000000
VERSION     DANJMI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.132"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(63..2555)
                     /locus_tag="PWU81_003029"
     CDS             complement(63..2555)
                     /locus_tag="PWU81_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL1111640.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2562..3029)
                     /locus_tag="PWU81_003030"
     CDS             complement(2562..3029)
                     /locus_tag="PWU81_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDL1111641.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT          838 a          697 c          518 g         1109 t
ORIGIN      
        1 cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt
       61 aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct
      121 tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg
      181 ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca
      241 ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata
      301 tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt
      361 tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt
      421 cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta
      481 acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt
      541 gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa
      601 tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact
      661 tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct
      721 tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc
      781 ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa
      841 agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca
      901 attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct
      961 ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag
     1021 cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc cgatacaact
     1081 gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt
     1141 gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca
     1201 ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct
     1261 agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct
     1321 gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca
     1381 gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca
     1441 tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact
     1501 tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca
     1561 tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata
     1621 ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca
     1681 atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct
     1741 tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg
     1801 cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca
     1861 gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt
     1921 cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc
     1981 ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct
     2041 tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca
     2101 gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg
     2161 atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc
     2221 aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa
     2281 gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg
     2341 ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc
     2401 atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca
     2461 tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat
     2521 actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa
     2581 cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg
     2641 aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt
     2701 atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca
     2761 tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca
     2821 attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta
     2881 aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt
     2941 tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca
     3001 attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct
     3061 taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt
     3121 tagaatcaac aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DANJMI010000126         2835 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000126 DANJMI010000000
VERSION     DANJMI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.133"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..1350
                     /locus_tag="PWU81_003031"
     CDS             <1..1350
                     /locus_tag="PWU81_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL1111642.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..2827
                     /locus_tag="PWU81_003032"
     CDS             1484..2827
                     /locus_tag="PWU81_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL1111643.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1111 a          389 c          549 g          786 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2761 tcctagtttt agccaaccct ataattttat tttaaaggca gtaacaggat taaacattga
     2821 aacataatcg ttact
//
LOCUS       DANJMI010000127         2675 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000127 DANJMI010000000
VERSION     DANJMI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.134"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            690..785
                     /locus_tag="PWU81_003033"
     CDS             690..785
                     /locus_tag="PWU81_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL1111644.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1087..1332)
                     /locus_tag="PWU81_003034"
     CDS             complement(1087..1332)
                     /locus_tag="PWU81_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111645.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1677..1961
                     /locus_tag="PWU81_003035"
     CDS             1677..1961
                     /locus_tag="PWU81_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111646.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1964..2380
                     /locus_tag="PWU81_003036"
     CDS             1964..2380
                     /locus_tag="PWU81_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111647.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
BASE COUNT          882 a          497 c          430 g          866 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tactcagata aaaagttgtt agtacccttc tgtatccgtt gggagagtaa
      241 ggctcaactg ccccctaaaa agggttctaa aaaatcttta aaaaataaat aaaataaacg
      301 cgcgcgcgcg agcaaaattt cccgccaaaa aatcacccac tttttaaaat aaacaggttc
      361 gcgcatgata ttaaactgcc aatctagctc cctatggtcg ctgtaagaag gagtgatcgc
      421 tacgctctca cattttactt gcagcagtcg cctttaggct ccttcgccaa aaataattca
      481 ttagggactc ctttgtggcg tcctgagagc tcgtctaagc cgttctaaga caagattcga
      541 taaaaactat gtccttcgtt ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac
      601 cccaaggcaa taaaaaactt aggcttattt ttgctttttt attgtataat tgattgtcgc
      661 ctatccgaca gtcttgcgac tggaaggagg tgtgtttcgt gttcactaac tttatttgct
      721 cccttcttgt tgggctttta agcgctttgt tcagttactg gttgaataac cgaaacaaga
      781 agtaaaactt aattaaggcg actcagccca caaaaaaagc cccagagaat ccgacctctc
      841 tggggctttc gtgtgtttcg tgttcactta tttgcaatca aattataaca gaaataaata
      901 tttatttcaa aaatatccta caaaagtcta aaatccgtaa ttatttctta tatgcaacat
      961 ttacaaataa agtcgctctg agagctcgta tttttcgatt taagacgttt tatttagttt
     1021 ttggtgtatt tcattggtcc aagctaaaaa actcttagaa gcgattttag agccatttaa
     1081 acccacttaa ttatattctc catgaaattc gctccatgcg tcaaaagctg cgtctgaaat
     1141 tccgccgtct tcgttcacta gttgcattaa tttttccaac gatcgttttc tttcgggtgt
     1201 catattttta tattccaaca atttttcgtc tgttaaatcg aacttgattg cttgaattgc
     1261 ttgctctaat ttttctaagt tttgaaaata ttcgtaagga aatttttcca ttttttcttt
     1321 ctccagtctc acaatttttc tagaaataaa atcacttctt tgccgtctat actctcaaaa
     1381 aaaatgtcgt ttgaaaaact aaataaattt ttgatcgtgc atttttaaat ttggttatcg
     1441 attacacgtg cagcactccc ccccgaattt taaagagcat aaggaaacgt gtacccactt
     1501 tctaaaaaaa gtgggtagat aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat
     1561 cactttttcg ccatcgtggg tagcttatgt atagcatttt ggcaaaaaac agctatacaa
     1621 atatagtatt tgtatggtat acataaatta tacataattg caaaaggaga ttttttatgg
     1681 acattttagt tcggaatatt gatccaaagt ttgttgcaac aatagacaaa cgttgccaag
     1741 aactcacgaa taaaacagga gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg
     1801 aaagtgattt tgaccgtcaa cttacgactt acaaaaaaga ccaattcgat ttattgctcg
     1861 ataaatttat tgctatccaa acagaaaata ctcaaatatt aagcgagtat gtctacacaa
     1921 ataatcaaat cattgaaatg cttatcggag gaatgatata accatgagaa aaacttctgt
     1981 ccaaaaacat gttactttgt accctagtca tgttgacgtt ttagattcca tcatcgaaaa
     2041 aaatccagat atcaataact actcggaagc cattcgcttt ttatgtatga gttatgaacc
     2101 cgataccaac acgaccacta ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat
     2161 ttccatcatg actgaaatca tcgcagaatt tgctgattta tatctaaaag atacaggcgt
     2221 tcttattggc acaaattcta aagtatatca agaagtgaaa gaaaaaatcg aagctaaact
     2281 aaatccatca aatcgaaatc gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac
     2341 ttctagaaat ttcgacccaa caaattttct aaaacgataa aaaacagata ggagttattc
     2401 ctatctgttt tttatttaga agccccatac aatatgctag aatgtactag caatcttagt
     2461 cggttgtaag ggacttgtct acttgcttaa tgtaaggggg gcgagttctt ttttatttat
     2521 tcttctaacc agttacgcag agaaatcttt ggcaaatctg acgttaggct tgaaaaagta
     2581 aattctaatt tggcgatttt attgcccttt tttgctttta tttttgtgac tttaagatca
     2641 ttgaagtatt tttttagttc tcgtaatgca ggatt
//
LOCUS       DANJMI010000128         2398 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000128 DANJMI010000000
VERSION     DANJMI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.135"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            207..2069
                     /locus_tag="PWU81_003037"
     CDS             207..2069
                     /locus_tag="PWU81_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL1111648.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="PWU81_003038"
     CDS             2069..>2398
                     /locus_tag="PWU81_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL1111649.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DANJMI010000129         2332 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000129 DANJMI010000000
VERSION     DANJMI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.136"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            24..1451
                     /locus_tag="PWU81_003039"
     CDS             24..1451
                     /locus_tag="PWU81_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL1111650.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            1453..>2332
                     /locus_tag="PWU81_003040"
     CDS             1453..>2332
                     /locus_tag="PWU81_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111651.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFE"
BASE COUNT          796 a          273 c          374 g          889 t
ORIGIN      
        1 aattttgaaa gttggaaata tagatgaata gaatagtacc tgttttagag aagagtttat
       61 taagtcgaga tgcgtttcga gtaataaatt taatcatttg tctgctttat tttatgccta
      121 gtacttttaa tgtaactaat atcccgatga aaatagcttt tgcttggggt ggaattatag
      181 ttttgtatga ggtttttata aagaaaaatt tttttaaaat gcgtttttct tggctactga
      241 ttggagcaat gattagtttt atcttaacta taatcattaa cagagaaaat tttttaattc
      301 catctattta taattttgga tatttgctga ttactttgat agtaattttt cctgcagact
      361 tttcacaaag tgaagaggaa aaaaagaaaa aaatggtgta ttttaataat gttttcatta
      421 tcataatttt tttagctgcg ctaatatcta tctatcaatt tatttttttg atttcttatc
      481 atgtcccaac aggaacgcca ggattacttg caagacaagg ttttattgaa catcggttat
      541 ttggtgtcta tacaagtcca aatgttggag ctatgtttgg atatacttct attattttaa
      601 gtttaattag tgtattaatt tcaaaaataa caaaaacgat tcgtgttttt tatatgatga
      661 attttatcgt acaaatattt tatattacac tttcttcgtc tagaggtgcc caggtagtaa
      721 tttgttcttt tttagtatta tttcttttgt tgtttgccaa ttcaaaattt agaaagagca
      781 ttagagaagt actacctgta aaaatcaaat ggatggtaag catattatta gttctattgg
      841 tatatttttc ttttggtact aattatgcta aggatttttt agcaacaatt cctgtatcga
      901 ttgatagaaa agtagaagtt atagagtcaa atgaatcaga taaaactcca cttccagaaa
      961 aagaagtaat attacagcat tctagtgata actccgaaat ttcagctggt cgttttacga
     1021 tttggaaagc tgcgcttaaa gttataagac caactttgtt atttggttat ggagagaccg
     1081 atttctatgg gaatagcgat gcaaaatttt tgaaaaaagt agatcttgat actacagatg
     1141 tgaatgaact aaaaagagca catggaaaca tgcataatgg atttcttcaa gtacttgtat
     1201 caagtggtat aattgctttt atatgtatct atgctttcta tgtgttaaat atctttagtt
     1261 tactcaaaac attttttaga agtaaagtga attatgcgtt tcttggtatt gttttaattt
     1321 ttatactatc tatttttata gatgaaatgg tggaggctca tgtacttttc aataagcgcg
     1381 atgtcatttc aattgttttc tggtattatt tgggaagcat ttcttacttg tatttaaaaa
     1441 aggagaatta atatgaagac agctttcatt gcttggactc ctttacattt gattaatata
     1501 ttaaatgttt atcaaaattt ttaccgagaa tcaaaaggag atttatttat ttattctgaa
     1561 tttgaaaacg ctagaagata tagtgagcgt ataaaagaat tagggctatt taatgatgtt
     1621 gtttttgtga atcaccggaa aatcggttct tcctttaaac gtaaaattaa cttgttaact
     1681 aaccataatc aatttttctc ggatgattta tccgtttatg atgaaatttt tatacagggg
     1741 gaaaattatt tcacaaaaat acttttctcc aatgtttaca aaaaaaatcc agatgtaaaa
     1801 ctaaattata tagaagatgg tttgggtact tatttgaaga agagtatatt ttcaacaagt
     1861 aaaaacgttc aaagagtttt taaaatatta aataaaaata gcatcttcaa aaaaaaaatt
     1921 gacgattatt ttgtctattg tccggaattg ttactttatg atgtaaaaaa tataaaaaaa
     1981 attcccaaaa tagatgaaaa cagtaaattg atgaaaaaca tacaatcaat ctttgggata
     2041 gaagaagttt ttgtcggaga taaatgtatc ttttttgatc aacctttatt atctgatggt
     2101 tatggcatag atgaaacaga tattttcgct aaagtaaata gtatattttc tggaaaaaaa
     2161 gaaactttaa ttaagcttca tcctagagca aggacaaatc gttatggtga aaaaaacata
     2221 ttgaattcca gtttaccgtt tgaagtcttg ctacttgaaa atgacttctc ttcaacactt
     2281 ttgattagtc ctatatctac aatttctttt tcaccatcta tgatgtttga aa
//
LOCUS       DANJMI010000130         2304 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000130 DANJMI010000000
VERSION     DANJMI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.137"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            192..446
                     /locus_tag="PWU81_003041"
     CDS             192..446
                     /locus_tag="PWU81_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL1111652.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            447..764
                     /locus_tag="PWU81_003042"
     CDS             447..764
                     /locus_tag="PWU81_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL1111653.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1323..2258
                     /locus_tag="PWU81_003043"
     CDS             1323..2258
                     /locus_tag="PWU81_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111654.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          840 a          311 c          434 g          719 t
ORIGIN      
        1 ggatgttaac ggaagtgtat ggtgagtagt tcactcgtat tttgatatcg tttgtatatg
       61 ttttcagcgt tctaagtggc ttattttgtc caataataat atggagtgga atcccttaag
      121 aaacaccgtt ggcaattgtt gttataacat gttataattt attcatcaaa aactaaaagg
      181 agtgtgataa catgggaaaa gaactaaagg ttagaaagat cggcaatagc gtgggagtga
      241 tccttccaag ctctcttgga ttaaaaagcg gagacattat ccaagccaaa caagaaggca
      301 acttattcat attagatact actcaaatag caaaagaaca cgatcgcaaa ttgattgaag
      361 aaagtttcca agactttgag aaaggtttaa cagtttcaga aattgaaatg gtaaaagctt
      421 tcggaaaata cggctggagt gaatagatga aagagtacac gctagtttat tctaaaagtt
      481 ttcagaccag ccttgaaacg ataatagaag aatgggaaaa tgagcttgct ctttctccag
      541 ccagcataaa aagatttgta ggtgcgatcc agaaggcttt agaactaacc aaaaattttc
      601 caactatgca tgaggaagtt tccgaaatct atggaatgga tatcccaaca tatcggatcg
      661 tgatcggcaa aagctatgcc ttattttatc ggatcgacca tgaaaaaaaa gagatacttg
      721 taggacgtat tttccaatct aaacaaatga aactaggctt ttaacaataa ttaggaaaaa
      781 gtcgctcatc ctctacaggt gggtgattaa tgccgctcga tatggttgtt aaccgtcggt
      841 tactcgaaga ttgatatata agacttagta cctttttatt ttgctccatg ttatggccga
      901 aggtctaaag atacaatcat tagtctagag aaaaattaag aaccgtagga actacgggga
      961 tcgcttggtg aataagagac actgctatta gtgaaaaaat aagatatcaa gtacgctcta
     1021 ttcccaaaaa tttcgtgact ttactcatga gaggttcaag tagtatattt ttaatcaaaa
     1081 ttgacgtaac ttttaataaa tactacctaa ataaggtagt atttattttt gtgttttaaa
     1141 agaggaagtc caatatatct atgggaaact tcttaaatat tatgttggta catgaaaata
     1201 tacatccctg ctagactatt tacaacattt tactttgtac gtattcttgt tttaaaagga
     1261 atgggataaa atataggtac aataactatt tgatgctaaa tataattaat ggaggagaga
     1321 tagtgaacat acttaatgaa aaaagatatg tatggtttgc tggtaaaaaa tatattacta
     1381 ctttcaaaag attatccgag ttaatgcagc agactgttaa aagtgctatg cctgaaggat
     1441 acaagtttaa ttttgttcaa tcttcagtat ctactgaaag tatctatttt tcaattcttt
     1501 ctgagagtag gctttttcat tttagaataa gttctcatgt aaagaaaact gatgttgata
     1561 tatatacgtt ccgtacaaat aattataaaa atttaaatga tttgagtaga agtatcagtg
     1621 aatatctcaa ggggaatagc tatctcaaga ttcgattttc acatatatta attttactac
     1681 catatttgaa tgagaatttc accctaattc aagtagataa gtctttcatt gcctcaggta
     1741 atcaaggaaa tatgaatatt acaagtaaca aaacgtcacg tgtgctattg gcgttgatgg
     1801 gttacggaat tctttataga gatgaccaaa atggccagat tctaataaat cgggctgggc
     1861 gttcattatt aatgaaatat agaaaattta taccggaaga ttttcaaaac ttacagttag
     1921 aaaacatgcg tttagaagat ttatttagat tagcagaaga tgataagtat agatttgaag
     1981 ttttgaaaat tcaaaagaat gaactattga ataagttata tcctgagaat gcgataaaag
     2041 cacattttgg tgatgctaat catatgtata gttggttcat tcctaaaaga tttagaggga
     2101 aaatcaatgc tggagatcaa gtgaaagtac tcaatcgaaa tagtacagat gtcgtaatga
     2161 taacagatat ctatgtagaa gatgaagata taatcaaata tatgaagcct gtgataaaac
     2221 ttgttaataa aaataaagga acccgtggtg ctagttaatt ttttcctaga gaaaaatgtc
     2281 taaacatgtt ggtaaatcaa caaa
//
LOCUS       DANJMI010000131         2293 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000131 DANJMI010000000
VERSION     DANJMI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.138"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(51..1598)
                     /locus_tag="PWU81_003044"
     CDS             complement(51..1598)
                     /locus_tag="PWU81_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDL1111655.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="PWU81_003045"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="PWU81_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDL1111656.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="PWU81_003046"
     CDS             complement(2043..2237)
                     /locus_tag="PWU81_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111657.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          644 a          477 c          408 g          764 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agt
//
LOCUS       DANJMI010000132         2253 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000132 DANJMI010000000
VERSION     DANJMI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.139"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            138..>2253
                     /gene="acm"
                     /locus_tag="PWU81_003047"
     CDS             138..>2253
                     /gene="acm"
                     /locus_tag="PWU81_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HDL1111658.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLL"
BASE COUNT          886 a          345 c          442 g          580 t
ORIGIN      
        1 tttataaata tttgtaaaat aactatcaat tttttagtta aaagattgga aatgagtggg
       61 ggaggaattg aagaaaggat ttttgagaga tgatatagta ggttttattt ttagttaaaa
      121 taggggagat agaaaaaatg aaaaagtgta gaaatattat tttatcaatg ttatttatta
      181 ttactaatat aacatcactg attcctgttc atgtctatgc ggatgcaggc agagatatca
      241 gcagtaatgt cacttcgttg accgtggacc caacgaatat tacagatgga ggaaacataa
      301 aagtaaaatt ctcgtttgac gaaaaaaaac aaaatatcca accaggtgat tatctttgga
      361 tcaattggcc aagtgaagga aatattcgcg gtgaaggatt ccaaaaagaa atcccattaa
      421 tgattgaaaa taaaaatgtt ggaactttaa cggtcagaaa agattctgcg caggttgtgt
      481 ttaatgaaaa tattaaaaac ctagactcag tagaaggttg ggggcaattt gagatacagg
      541 ccagaaacgt aaccgatacc ggtgaagaaa atacgggacc attcaccgtg acgagcggtg
      601 ataaaacagc tactgtaaat gtgacaaagc cagcttctgg ttcttcttct agcgtttttt
      661 attataaaac aggagacatg ctacctgaag atactaagca cattcgatgg tttttgaata
      721 ttaacaataa cggcacttat gttgaacaac cagttaaaat cagtgatgaa atccaaagtg
      781 gacaaagatt agatccaagt acgtttgaaa ttaatcaaat acacttaggt gaacagaagg
      841 tttatagagg agaagaggga atccaacaat ttttacaaga tttccccagc gctacattta
      901 atttttcagt aactgataat tacattgaaa taactatacc aaaaaacttt gtcaatctta
      961 ggaaaataat ggtgagttat aagacgatca tagaaaaccc agaacaaata aattttgaga
     1021 atcattcgga agcttggttt aaagaattta ataaacctgc tgttgatgga gaaagtttca
     1081 atcatacagt taaaaatatt tcagcttctg gtggagtcaa cggtacagtt agaggcgaat
     1141 tgaagatttt caaatatatc aatgacacag agattggtat cccaaatgtg actttcgaat
     1201 taagacgagc agatgaacag cctattcaag gacagtctag tatcctgttg acttcgaatg
     1261 aacagggaga agctagtatc aaaggcctgc aagtaggaga ctatgttgta aaagaaaaag
     1321 aagcacccaa ttggattgat tttgatccac taagttcaaa tgaactcaaa ttctccataa
     1381 acgaaaatga tacagaggga gtcagtttac ctatttataa caagaaaaag gttacgaaca
     1441 tcacagcaac taaaatttgg aacggtggaa ccactccaag accgagtatt tattttaaat
     1501 tattcagagc aagtacaaac aattgggaac cagttcctga tgcagaaaca aaacgattag
     1561 acaatggaat aacttcagtg acttgggaag atatccaaca gtatgatgat agtggaaacg
     1621 aatatacgtt taaagtgcaa gaagtagatc aaaacggaaa tgattatgtc ccttcaggtt
     1681 atcaaaaaat tgaaaacggg ctagtcgtta caaatgagaa taaagaagtc atttcagtct
     1741 caggacaaaa aacatgggaa gacaatgaga accaagacgg caaacgaccg acgacgatca
     1801 cagtaaatct attagccgac ggtcaaccga tccaacacaa agaagtaagt gaaaaagatg
     1861 attggcgcta tcgtttcaca aatctgccaa aatacagaga cgggcaagaa atcatctata
     1921 ctgtaacgga agacaacgtg cccgaataca gtacgacgat tgacgggtat aacatcaaaa
     1981 acagttatac accggaaaaa acaaatatat ctataccagg tagccatata acaccgtgga
     2041 aaaattttcc agagaagaca gagagagaga aaattagaaa tctattctct caaaaattgc
     2101 aaatatttgc tcatacagga gaatccagcg aacaaagaag caattatatt agtaagagag
     2161 ctgtacctaa acagataaca aataaatcaa aaagcattct tcctaaaaca agtagtaaaa
     2221 aatctagttt tgctgtaatc attggtttat tga
//
LOCUS       DANJMI010000133         2049 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000133 DANJMI010000000
VERSION     DANJMI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.140"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            662..994
                     /locus_tag="PWU81_003048"
     CDS             662..994
                     /locus_tag="PWU81_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDL1111659.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1613..1954
                     /locus_tag="PWU81_003049"
     CDS             1613..1954
                     /locus_tag="PWU81_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HDL1111660.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          716 a          271 c          431 g          631 t
ORIGIN      
        1 ataagtgtta aaagaaataa ttaatttcaa atatagaaaa gggaacgtat gtttgcatac
       61 tccttttttt tgagttataa taatacgcga agagagataa tttcattatt taaacaagct
      121 aaaaatcttg atattacttc ttttcaggca atacgaatat tattcgctta cattttttgt
      181 ataaatttaa ttgtctgcta tgcttgtgat gaattgattt tcgttttgca cttttggatg
      241 ataaatttgg acaattgaaa atacaaaaaa aagaaaggcg gtatgaactc ggaaggctgg
      301 taaccttcgt cagcgttcat acacggcatt aagatttggc ccttaatgcg ttaacctttc
      361 agtgttacgg tattacccgc aatggattta gtataacaga tattcaaaag tgataccact
      421 ttttttggct gtgttaaggt taaaaatacg tgatattacg atgaatctat gcctaagcat
      481 aatgttataa aaatgtccgt gtggctcttt gccgtatgac gaacgctgaa attagaggga
      541 acgcatgttt ggatgcgttc cctctttttt gtttgtcatg aacgaaaatc aattctctat
      601 ttttgaaaaa taacaagaaa aacttgttaa aaatatttaa agaaattggg tggtttattt
      661 tatgtcatat acaggaattt tgagtctcaa agatatttgt cactacggta aacggtgtac
      721 cgctacagag aaaatcacga agaaactatc aactggccaa agtaaaaccg ttgtgcagtg
      781 taagaaatat ataatccaga aagacaaagt ctcagaggaa atgatttatt acattgggaa
      841 acaaaagcaa ataattttaa aagatcctat ccccttaaaa gagctatatc caacaatcaa
      901 acatgtttat gaccaaaacg gtgtactgat tggtcgaaga aaaaatggag tcttgcgttg
      961 taccgcaaaa gggatggggc gtttgatcgg ctgaattgat cattctcata acttgtattt
     1021 tatcgttttt cgcttgcttg aaaatattgt cgagacttct aaaggaactt agagtcacta
     1081 gaaaaataag gagtagactt gatctatcaa caattccctt cggggagtga cggggtaggt
     1141 tttaacgatt ggtaacgtaa aaaattggtc aatcgagtaa atgcgcttgt ttggatagtg
     1201 gtgaaatgaa tttgtgtaaa taataagcgc agagtaaaca agagggaatt tagaagttag
     1261 acaagttaga atagcgtcta gattaatcat gagcagctac taagggatct agactttgac
     1321 gcaaactcat ggaataagga gtaggtcttc tcgtaatggg gaggacgcac aagaaattgt
     1381 gtcgtgtgct gcagtacatg tagatacttg cgaggacgtt gattattgga tcaatcgagc
     1441 atttaccgct gtctactagg gtttctcgtt tcggacgcca taaacgagtc gaaatttttt
     1501 atgtattttt aagtaaacga aaaacgataa aatatgaaca agtcctgaat aaagtgaaaa
     1561 aattgatata aaacatagag aaaatgaaat ttacgtaatg gaggaatgac aaatgaaaaa
     1621 ttatgatccg aatatacgat tgggcacaca tactattaag gtttctttcc aacgttggga
     1681 ttacaaagga tttgtgacgt ttagaagggg cggaaattgt aaaggattag atgttttagc
     1741 attagacgag gatgatttat acgatcaaac attgactgat aatccaattg gattcggctt
     1801 gctacctgaa gatgatgaag gtaatgaatg gtttaaaatg acgcttatga atgataatgg
     1861 agacgaatta tctgttgaag atacttggtc atatttgagt gattatattg tcagctttga
     1921 aatcattgaa tttgtagcgg ataaagaaga ataaaaactg gaggaataaa ttttacagat
     1981 aaaggcttag aaggagtcac gacgattaat aaaaaggtag tattacctta ggcattgagt
     2041 aaggaattt
//
LOCUS       DANJMI010000134         2005 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000134 DANJMI010000000
VERSION     DANJMI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.141"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            272..838
                     /gene="amaP"
                     /locus_tag="PWU81_003050"
     CDS             272..838
                     /gene="amaP"
                     /locus_tag="PWU81_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL1111661.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            851..1039
                     /locus_tag="PWU81_003051"
     CDS             851..1039
                     /locus_tag="PWU81_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL1111662.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1052..1609
                     /locus_tag="PWU81_003052"
     CDS             1052..1609
                     /locus_tag="PWU81_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL1111663.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1641..1883
                     /locus_tag="PWU81_003053"
     CDS             1641..1883
                     /locus_tag="PWU81_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL1111664.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          731 a          309 c          407 g          558 t
ORIGIN      
        1 ataaatagaa tcttgtaata ataactgagc ataatctttg caatttttaa tcttgaataa
       61 tatcctttaa gtatagccag caatcaaaag taaatagaga ataagtaagc aattgttatt
      121 tcagtatcag actggcaata aaacgccgtt atgatccagc aatgtttggt agcgctttat
      181 ttactgatgc tatgctaact gttcgctatc tatactgtga ttgtcggtag agttatttac
      241 agggtaaaaa taagtttata ggaggtggaa aatgaataag gggttaaaaa taatcggtgt
      301 tatcatgagt ttactgctac tttcagtatt tctagcgatt gcgttgatca atgcgccgta
      361 tattatgcct gaacaattgg aacgatttcg cttctttact ttaacgaatt actacatgca
      421 acaatatatt ttctgggcag ctgtagtatt tgccgttctt gctattatca tcttgctagt
      481 cgttcttttc tatccaaagt caagaggaac tttcgttatg aaacgggaag atggcaaact
      541 aacaatcgac aaaaaagcga tcgaagggtt ggtacgttcc catttacacg aagaagagtt
      601 cattcattca cctaaagtac ggatacgttc aacgaaaaat cgaatccata tccatgtcaa
      661 cggagacttg aaacgaactt cttcattagt agggaaaaca ggtgtattga tgcaagatat
      721 cgaagaagaa gtaactaaag tactcggaac aaaagaaacg gtcaaagtgg cagtcacata
      781 cagcggttat gaagaacaag aagatacaag agactacaag cactctcgag tggaatagga
      841 gggccaaata atgaaagaac tattttcagc atacaagctc cctattatct ttgggggtct
      901 tggactactg ctagctgttt tatggttaac gattggattt ttcaagacat tgttgatcat
      961 aatcatgaca gcaattggcg caggaatcgg tttttatttg aaacagtcag gaatagttga
     1021 tcaatttttt aaatcatagg aggaaacaaa gatggaaaac aaaaacatga atacaacaaa
     1081 tggatcagtt ttggcaaatg aaaaaggaga aaaccgcatg atgaatggtg aattgactta
     1141 tgaagacaaa gtcatccaaa aaatcatcgg ttatgcattg aaagatgtag atggtctatt
     1201 gacattagat ggcggatttt tctcaaatat ggctgaaaaa ttagtcaaca cagataacgt
     1261 aacagcaggt atcaatacag aagtaggtaa gaaacaagta gctgtggatc tagatgtgat
     1321 cgtagaatac ggtaaagaca tcgaagatat ttacaaccaa atcaaagaat tgatcagtac
     1381 tgaagtaaac aatatgacac atttagatgt tatcgaagtg aatgtgaatg ttgcagacat
     1441 caaaacacaa gaagaatacc aaaaagatca agaaacagta caagacaaag taacagaagc
     1501 agcaaaatcc acaggacaat ttgcttcacg ccaaacagat aaagcaaaat ctgctgtagg
     1561 caaaggtgca caaaaagtca aagaaaataa cgaaccacgt gtacaataag aagtaagaag
     1621 tcagttagga ggaatttaaa atgggattta tttgggcatt aatcgtcggc ggtatcattg
     1681 gagcaattgc aggagcaatc acgaataaag gatcatcaat ggggatcatc gcaaacatca
     1741 ttgctggttt agtaggttca gcaattggac aagctttact aggaacttgg ggaccaacaa
     1801 ttgcaggaat ggctatcatt ccatcattga tcggtgcaat cattttagta ttagtcgttt
     1861 cattctttgc acgtaaatta taagcaaatt gaagaaaaac ttatgttgaa atgaagaaca
     1921 aatttgagaa ccgtagcgac actcgctgcg gttctttttt atatatttta tatgaagtat
     1981 tctttattcc ttatgaaaaa atatc
//
LOCUS       DANJMI010000135         1859 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000135 DANJMI010000000
VERSION     DANJMI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.142"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="PWU81_003054"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="PWU81_003054"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL1111665.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DANJMI010000136         1750 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000136 DANJMI010000000
VERSION     DANJMI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.143"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(145..459)
                     /locus_tag="PWU81_003055"
     CDS             complement(145..459)
                     /locus_tag="PWU81_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111666.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            745..984
                     /locus_tag="PWU81_003056"
     CDS             745..984
                     /locus_tag="PWU81_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111667.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(1346..1606)
                     /locus_tag="PWU81_003057"
     CDS             complement(1346..1606)
                     /locus_tag="PWU81_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111668.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
BASE COUNT          614 a          303 c          231 g          602 t
ORIGIN      
        1 tataaactct agagaaatta attcatccta acataaaata attgttcact caaaagtttt
       61 tctagaacaa ttaaaacagc aggcttcaca ttcgctatac atttgtaaga agtaataatt
      121 cctgtactaa aaggcataat tgctttaatt attttttgac ttctcttcaa acacaagaag
      181 tagttgtgta ataattgcag aaataaggat tccaataata gatataaaat ttgacatgga
      241 aacgtctata acattaaaag aataaagtgt tgaagaaagt aaactgattg aaataacccc
      301 tccgtaaaaa atagtctgtg aatataccca ctttttttca gaagacgtac ttcgtttggt
      361 tctaaaacct atccctgcat ttctactctt atcaaaaaac caaatattta gaagtaataa
      421 tattgctatt attaagataa aattactgta aaaatccaaa agctatcctc ctagcaacta
      481 tttaaatacc caaaaaaatt acctctagat aatatcatac ctacgttttc acttataaat
      541 tttttcataa aaaaagcgtt tagaggcata atcgttaata cagatggtaa ccaatgtatc
      601 tggtttgaac tgggatatca ccctctccat atatgtatta ctaagactaa tagatactat
      661 aaaagacttg atagttttgc attgattttg cctaatactg agcataggtg tatgctaaac
      721 gatactataa ggagtgtgta cataatgaaa agaattctgt ttatcagttt atcaaattta
      781 ttgttactta ctgcttgtgg gacttctgca tcaaagaaca taccttcaga tacgcaaatt
      841 tcgaatagta tcgattcaac aatttctaca aatgagaaaa attctccggt agaaacttcc
      901 gatataaaag tttctacctc tgcaaaaaac agggtaaaga aactgtcagc acgtctcctt
      961 tatttcaaac aaactctgaa ttagcttctt catcttcttc agcaaataat gatgatagtt
     1021 tcgcgtttac tcctaactaa atgcaacctt tacaaatgct ttacgataac aagcctgagc
     1081 taaatgatcc tgatatcagt tttgcatttt ttgaaatgat tggatcggat gatcttttta
     1141 aaactactag tttgtctatc cgaaaatagg acagttctgg cagtataggt ttttatcgag
     1201 tttctccacc aggcctcgtt acagagacag attctctagg cgaacctttt gaataaatca
     1261 aaaatcatga acaatcactg ccaactaaat aaagtgcctt caaaagcttg gtcttctcgc
     1321 ttttgaaggc actttattta aatctttatg aaactaaagt atgttcatat ttgttgctat
     1381 agttatatag atattctgtt gtttccaact cataaacgtg agtatatttt gccactactt
     1441 ttccgcattt ttctaatatt tcattgcgat cgacgtcatg atattcgtct acgcagatca
     1501 caacacaatt ttctaattta ctgattactt ctccaatcac tttttgtttc aagccaatag
     1561 gttggcaaat atattttttt cctaaaatga tagtttcgtt attcatattt catccatcct
     1621 taatgttttt tcattattta aaatgataat aacattaaaa tatgattatg aaaatcaata
     1681 acacttgtga ttttataaaa aaataaaaaa accgaaaaac agaaatgccc actgtctttc
     1741 cggctctttt
//
LOCUS       DANJMI010000137         1655 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000137 DANJMI010000000
VERSION     DANJMI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.144"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            144..1610
                     /locus_tag="PWU81_003058"
     CDS             144..1610
                     /locus_tag="PWU81_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDL1111669.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          462 a          297 c          338 g          558 t
ORIGIN      
        1 ttcttttttt tatgatattg ccattaaaaa ttacttgctt ttatagaaaa ttagtttata
       61 tttcttaaat aagtttttta gttaaatttt tcggtaaaga tgtataatat tttctgaaaa
      121 tttaaagaat tggggtatag attatggaaa aacagcaaga caagggcttc ttcgggcagc
      181 cgaagggatt gtcaactttg ttcttcactg agatgtggga acgatttagt tattacggta
      241 tgcgtgcatt acttatctat tatatgtacg atacagttgc caacggggga cttggattac
      301 cacgagcaac agcattagct atcatgtcga tctacggatc tttgatctac atgtctagta
      361 tcgtcggcgg atgggtatct gaccgagtgt taggagcaaa acgcactgta ttcttcggtg
      421 gagttttcat tatgcttggc cacatcgtct tggctttacc atttggtgta agtacattgt
      481 ttgtttcgat ggcattgatt attatcggaa ccgccttttt gaaaccaaat gtttctggca
      541 tggtcggaca tctttattca aagactgact tgcgaagaga tgcaggattt tcgatttttt
      601 acatgggtgt caacttaggg gcattattag caccactaat cgttggtaca atcggtcaag
      661 aatataacta tcatttaggg ttctctttag ctgctattgg gatgttcttt ggattattac
      721 aatacatcat ccaaggacgc aagacattag acggagtggg tacaacaccg ccgaatcctt
      781 tgacaactga agaacgtaaa ggattcatcc gtaacttgat catcgcactt gttgttatcg
      841 ctgcagtatt cggcggggca caactaactg gtcatctgac aattgacttt tttgtcaata
      901 caatcagtgt attggggatt atcttaccgt tgtattactt tatcaaaatg ctgacttcaa
      961 aagatgtaac tgctgaagaa aaaccaagag tatgggctta tatcccatta ttccttgcag
     1021 caatcatttt ctggtcatta caagaacaag gttcatctat cttagcttta tttgctagtg
     1081 aacgtacaca atctacgcta tttggtgtaa cgatccaagc aagctggtat caatcattga
     1141 atccaatttt catctttatt ctgacaccag tattcgtaac gatgtggacg aaacttggtg
     1201 aacgtcaacc atctacagta gtgaaattct ctcttggact tttatttgca ggattatcgt
     1261 acttgctatt gatgttgccg ggtatgcttt atggtacaag tggaaaagta agtccattgt
     1321 ggttgttggg aagtttcttc atcgtggtta ttgctgaatt gtgtctttca ccagttggtt
     1381 tgtctgtgac aacaaaatta gcaccacgag catttgaatc acaaacaatc gctatttggt
     1441 tcttagctga tgcttcttca caagcaatca atgcacaaat tgcacgcttc tacacaccgg
     1501 gaacagaatc tgcttacttc ggtattatcg gtgcagtagc aatcgtcggt ggtatcttgc
     1561 tatttactgt gaaagaacca atcaaaaaac taatgggctc tattcattaa tcaatgaaca
     1621 taaaaacagg aattgcttat ttgcagcagt tcctg
//
LOCUS       DANJMI010000138         1627 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000138 DANJMI010000000
VERSION     DANJMI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.145"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            798..>1627
                     /locus_tag="PWU81_003059"
     CDS             798..>1627
                     /locus_tag="PWU81_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL1111670.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          577 a          222 c          248 g          580 t
ORIGIN      
        1 taatttagtg ttttgtttgt aattcctaat tttgctccac ttttagataa tttttaggtc
       61 tatttaactc atcagacatt ctatgctcat ttttctccaa atttgtttca actctttcgt
      121 acttaatttt gcgtatttga aaattcttct tttagagtac ttttattacg atactagtat
      181 gatatatatc tatagcaaac agttaaaatc ttcctaaaat aagcgaatag cattcctaaa
      241 acctataaaa tttttagatg aagcgacaag tggacaatta ataaactact tgaacatgaa
      301 attatggatc acctttcaaa tttagaaaat ggaattctta tacttattgc gcaacattta
      361 ttgattgcaa aattatgtga caaaaaaaac tagtagaaga aagaataatg gcttgataat
      421 aaagtactac ctattaaagg tagtacttta tttaatttag tataaattaa catactacta
      481 tattttaggt ttcatattat ataaattaat aatatactac catttgttgg tagtataatt
      541 taaaggtaat aagttattct tttattacta tgtttgggtt ttatagcaaa gaaagtttaa
      601 taagagtttg gacctagcaa acaatattaa tgaggtcatt aaatcttatt atctttagtg
      661 atataacgaa atatctacac agacaaaatg atagcgtaaa atattttgtg taaatagata
      721 tttagggtag aaaaaaagaa agtccattct gtaaaattaa agttaccaca accgaaattt
      781 tataggagga ctttctcatg cccaatttta ctacagaaat tatggaaaca ctaatcaata
      841 aaggtgattt agatgaatta tttcgatgtc atttagaatt agctgttaat tcattattgc
      901 aggctgaatt aacagctttt cttgattatg aaaaatatga tcgtgctggg ttcaattcag
      961 gtaattcccg taatgggaat tattcacgtt cattcagaac agagtatgga gaattaaatt
     1021 tggtaattcc tagagataga aatggtaagt tttctcaaca aactttacct gcttataaaa
     1081 gaaccaatga ctctttagaa accactatta ttcagctatt tcaaaaagga attactatgt
     1141 ctgaaatctc tgaattgatt gaaaaaatgt acgggcatca ctatacgcca caaaccattt
     1201 caaatatgac taaaatagtt tctgaagata ttattgcctt taaagaaagg tccttagaat
     1261 ccagatactc tgtcattttt atggatgcta ctcacattcc tttaaaaaga caaactgttt
     1321 caaaagaagc agtttatatt gtaattggca ttcgattaga tggaaccaaa gaggttcttg
     1381 ggtttagtct tgctccaact gaatctgctt atgtttggaa agagattctt caagacctaa
     1441 aagaccgtgg tttaaaagag gttttattag tcgtaactga tggtttaagt ggcattaacg
     1501 atagtattca tagtgtttat ccaaatgctc aatttcaaca atgttgtgtc catatatcta
     1561 gaaatatcgc tcataaggtt cgtgttagtg atcgacaaga aatctgtagc gatttcaaat
     1621 tagttta
//
LOCUS       DANJMI010000139         1599 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000139 DANJMI010000000
VERSION     DANJMI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.146"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(<1..212)
                     /locus_tag="PWU81_003060"
     CDS             complement(<1..212)
                     /locus_tag="PWU81_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL1111671.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEF"
     gene            complement(209..862)
                     /locus_tag="PWU81_003061"
     CDS             complement(209..862)
                     /locus_tag="PWU81_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL1111672.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1150..>1599
                     /locus_tag="PWU81_003062"
     CDS             1150..>1599
                     /locus_tag="PWU81_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1111673.1"
                     /translation="MDNHTRKLLVLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQ"
BASE COUNT          522 a          284 c          204 g          589 t
ORIGIN      
        1 taaaactcca aagtttgttc tatatatttt atttcttttc cattcatatt actagaaatt
       61 tcatttgcca tattatgaat cttttctttt ctcgtctcca aatatgtttt aggaaagata
      121 aaaaaaatta atgaatgaac taatataatt attattccaa gaacagaaaa tatttgtatg
      181 aacatttttg gaaatatctt taattttttc attttctctc caatttatat cctacatttc
      241 ttatagtagt tatacagtct aattgtaatt tttttctaag ttcttttata tatacatcta
      301 ttactcgatc ataaggagtt tcttcgctat ctttccatac ataatcaata atttgcattc
      361 ttgttaacac ttgtccatta ttatccaata aacattttaa tacttctagt tcttttgcat
      421 ttacatctat ttcttcacca tttattttag ctgtatagct attaaaatta accgatagat
      481 ccttatattc aaacttctct aaatgtccat aattcttctt aattaaagaa tctattctag
      541 cttttaagac cggcaatgaa aatggctttt ctacatatcc gtctactaaa ttagtaaatg
      601 catcaatttt atattcttca tcactaaatg cagtcaagat tagaattggt aaattgcttt
      661 tctttctaat ttctttcaac acttctaaac catttataaa aggtatctga atgtctaaga
      721 taaccaaatt tatatcgcta ttaaattttg acaaggcttc tcttccatct ttagcttgaa
      781 taacagtata tccaaattct gaaagatatt cacttattcc ctctcttatc atattatcat
      841 cttcaacagt taatattttc atatatacct ccacttaagc ctatacacta gatattatac
      901 agcatattat ggctctataa tatctagtgt tggtgacacc ttctgttgcc aatacttttt
      961 cttttttcta ataaatgcaa acagacacct tgaaatcctt tagaataaag ggttctataa
     1021 tattttgtgt tgttggaaaa tcgaaggcga ttttctacta acactttttc ttcgtttcaa
     1081 ctcaaaaaga gactcacttt tggtaaaatt tagtcgacca aaacatcacc ctaccaaagg
     1141 agtcgctcta tggataatca tactagaaaa ttactcgtat taacagataa acatttattt
     1201 tttgatgaag aatggctgat tgaaaaagaa tataaaggcg ttcaagcgca gtttattaaa
     1261 gggagattgg atgactcacc ttcccattgt gaacactgtg gatcgattcg tattatccgc
     1321 aacggttcct atacgacacg tactcagata ttgaaagtca aagaaaagtt gactatttta
     1381 gaattaaaac gtacccgttt tctatgctat gattgtgggc agaccttttc tgcgaaaacg
     1441 gacttagttg atgaacatca tcaactaaca aaggaactga agcaggcgat tctcatagaa
     1501 ctgtacgaaa atcaatcacg taaactaatc gctaagaagt acttcgtctc ggatgggacg
     1561 gtgacacgga tcttgcgtga agcaacgaag cattaccag
//
LOCUS       DANJMI010000140         1583 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000140 DANJMI010000000
VERSION     DANJMI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.147"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            81..>1583
                     /locus_tag="PWU81_003063"
     CDS             81..>1583
                     /locus_tag="PWU81_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL1111674.1"
                     /translation="MDPTVFEAEEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNRLARKRAEFKQFGKQIDLVIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKTKGLIVTFRKPATMEKN
                     VEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIGVYETVKGSLMEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHAFSNEWFVSEKELHASNLQYM
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIP"
BASE COUNT          575 a          265 c          321 g          422 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt cca
//
LOCUS       DANJMI010000141         1480 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000141 DANJMI010000000
VERSION     DANJMI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.148"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            64..972
                     /locus_tag="PWU81_003064"
     CDS             64..972
                     /locus_tag="PWU81_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HDL1111675.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="PWU81_003065"
     CDS             972..>1480
                     /locus_tag="PWU81_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111676.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DANJMI010000142         1441 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000142 DANJMI010000000
VERSION     DANJMI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.149"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..387
                     /locus_tag="PWU81_003066"
     CDS             <1..387
                     /locus_tag="PWU81_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1111677.1"
                     /translation="DRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLI
                     ENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDT
                     YNQKFLNRSHKGFQQVTDTLVSMFTE"
     gene            810..1441
                     /locus_tag="PWU81_003067"
                     /pseudo
     CDS             810..1441
                     /locus_tag="PWU81_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          509 a          262 c          251 g          419 t
ORIGIN      
        1 gaccgaaaag aaatctgtga tgactttaag gctgtttatc aagctaactc aaaagaagaa
       61 gcgaatacct tcttatccgg catgattgag aaatggaaga aaaactatcc taaagtgacg
      121 cagtcactca tagaaaacca agacttatta actttttatg attttccacc tagcattcgt
      181 agaaccattt actcaaccaa tctaatcgag tctttcaata agcaaattaa aagatacagc
      241 cgtagaaaag agcagtttca aaatgaagaa tcactagaac gctttctagt cagcattttt
      301 gatacataca atcaaaaatt tctaaacaga agccataaag gttttcaaca ggtaaccgat
      361 acattagttt caatgtttac tgagtaacta attattttgc aggaggacaa tttatttaca
      421 caaaattatt gacgctccct gtttcaaagt tagtagagaa aatgtagtta tatatttaaa
      481 tttttaccag aaagaaatga acaccttcaa ttatctaaat ctcgaaaatg gttagatgta
      541 atcatcagga aaaacaatct taaacgaatt actattcatg gtcttagaca cacacatgct
      601 agcttgcttt tagcaagcta gtgcaaatat taaagatgtg caagagcgtt taggccactc
      661 ttctattcaa atcattatgg atctatatat tcatattaca gacaaaagaa aagaagaaac
      721 cgtagcgcaa ttcgcaaaat ataggctcta taatttatgg gactgatgaa tcaaaattga
      781 ttcttcagtc ccattgttat tttaggtata tgattcgtac attgaaagag ttgaaaaact
      841 atcttccgaa gccgtcaaaa aggaccctgc caaaagtatt gatggtggat gaatttcgtt
      901 cgcacgcttc aattgtggat aaaatgagct ttatttgtgc ggatggcgag actggaaaac
      961 tcattgatat tttgcctaca cgaaaattgt ctcgattaac acactatttt agtcgctgtg
     1021 caaatcctga agcggtggaa tatttagtga cagatatgaa cgccgcctac ttccaactga
     1081 caaaacgtgt cttaccaaat gcgaaagtcg tcattgattg ttttcatatc atcaaacatt
     1141 tgaatcaggc gttcaatgat tttcgtgtac aagaaatgaa aagattacac cagttagggc
     1201 aacgacggga agccgagaaa gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa
     1261 aaatcaatca ctatgattac aaaacatggc gcagttttcg tgcacctaaa tacccgctac
     1321 tcactgaagc aatgatgatt gatcgattgc tggcattttc aacgccttta aaggacgcct
     1381 atactttttt ccatgagctg accgaagcct ttagagacaa ggaatcagac ttatttttta
     1441 c
//
LOCUS       DANJMI010000143         1419 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000143 DANJMI010000000
VERSION     DANJMI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.150"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="PWU81_003068"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="PWU81_003068"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDL1111678.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          494 a          184 c          236 g          505 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact attactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tggactaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgacgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg
     1141 tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac
     1201 ttaatccata gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca
     1261 ccccaaacca cttccccata agtgtattac gctttaaact cttttatatt tgaattcact
     1321 aagaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttataatt aggatttaga
     1381 gtaattaatt agatgtttat tttataggac tgatactat
//
LOCUS       DANJMI010000144         1369 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000144 DANJMI010000000
VERSION     DANJMI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.151"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            309..1349
                     /locus_tag="PWU81_003069"
     CDS             309..1349
                     /locus_tag="PWU81_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL1111679.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          539 a          187 c          218 g          425 t
ORIGIN      
        1 tttatatgca taaggtagtt cttaatttat tttttgtttt gacactggta gtacttaatt
       61 atctttaatt ttaaaactaa attttattca tacgtttttc tggtagtatt taattataaa
      121 taatatttat ttaaagtagt tcttaatgac tttaaaggta gtacataatt cattttattt
      181 ctgattttag gtagtattta attaaaaaac agttttaatg cgtttgcatt ttttatttat
      241 ctttgctatt ttcgaaaaga gtaggtaatt ttacataaat aaaacaacgt gaaagtagag
      301 gatctacaat gactgatttt aaatttttta aggcggatag agtttacaac gaattatttt
      361 atcaatttcc aaaagtcttt attgtttctg acgaatacaa aaaaatgaaa gattcaacta
      421 agattgccta tatgctttta aaagcaagat tagagatcgc aatcagcaaa cggcaaatcg
      481 atgaagaagg taatgtttat tttacttata cgacaaatga actatgtaga gtattaaact
      541 gccaaaaaca aaaagcgata gcaatcaaaa aagagttgga atcctttggt ttattattac
      601 aaaagcagat gggatttaac aaacagttag ggaaaaataa tcctaataga ctatatctag
      661 cagaattaaa agtctcagaa aatgatatct acttactcga aaaatttgat agagagaata
      721 gggaaaacgt tgataaatca gagggtatga aaatcatacc caccctcgac gaaaaatcag
      781 acgctgaatc ccttggggct caagagggta tgaaaatcat accgtgccaa aacgttgata
      841 aatcagaggg tatgaaaatc ataccagaac ttaataataa tatattagac actaatagac
      901 acaatataga cactgaaaaa gaccgcctac aagatcaatt gttgttagac aattttgaga
      961 caattatgac aaacgacagc attgctacgt ttgtccctga acgagtatta aatttgataa
     1021 aaacattttc ttcaagttac agtgaagctc aaaaaaccgt ccagactatt cataatgcaa
     1081 agaaaaaagc tgaaatagaa agtgatattt cgatagtttt tgaagaactc gatagttatt
     1141 atgtcaatgc agaacaagaa ttatacacga cactgttaaa agcctatcaa aaattaaaaa
     1201 ccgaaaaagt cgaaaatatc cagaacctga tttttgtcta tgtaaaaaat tggtttatcg
     1261 aaaaaccaat agctgctaaa gtatcaagtg aaaaacgttt gaattatgca agctccccaa
     1321 gcactattac gaaagactgg ttagagtgaa actttactga caaagtgat
//
LOCUS       DANJMI010000145         1368 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000145 DANJMI010000000
VERSION     DANJMI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.152"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            82..>1368
                     /locus_tag="PWU81_003070"
     CDS             82..>1368
                     /locus_tag="PWU81_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HDL1111680.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIN"
BASE COUNT          467 a          263 c          257 g          381 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taatcaat
//
LOCUS       DANJMI010000146         1355 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000146 DANJMI010000000
VERSION     DANJMI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.153"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..1355
                     /locus_tag="PWU81_003071"
     rRNA            <1..1355
                     /locus_tag="PWU81_003071"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DANJMI010000147         1312 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000147 DANJMI010000000
VERSION     DANJMI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.154"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(88..161)
                     /locus_tag="PWU81_003072"
     tRNA            complement(88..161)
                     /locus_tag="PWU81_003072"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(125..127),aa:Ile,seq:cat)
     gene            complement(350..1309)
                     /locus_tag="PWU81_003073"
     CDS             complement(350..1309)
                     /locus_tag="PWU81_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL1111681.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          265 c          248 g          431 t
ORIGIN      
        1 catttgagtg gcaatgaaga aaagctttag cttgtataat actcttcaaa aaattctaac
       61 acagaacgat tcaaatggct acgttaatgt accctgtagg actcgaacct acgaccggac
      121 ggttatgagc cgtctgctct gaccaactga gctaagggta ctggttgttg ccacataaag
      181 ccataaacaa tcaaccagta gaatgtgtgg caacaaacct gttatcgcat agcttggagt
      241 gtgactatta tgggtgatag tgaagatatg cgataacatc actattttat cgaatgattt
      301 ttatagttgt caatatagtt atgtaatgct cctcaacgag gagctatttt tatcgtttag
      361 gaatatttaa ataccaacgt ttgtcatgga aatcttgcgc accgccttta gtgtttccct
      421 ctggatcatt cgttgcccgc atcatgacat agactttctt attagggaag ttacgcatgt
      481 taaaagatac atgatagcca acatttccag aagtattata agcttgattt acatctgctc
      541 tataaattcc atcagctctt actcgagcta attctttccc agtattgtaa tccataatga
      601 agatatactc gtatttatag ttagcaatgt gccatccagc tacatgcaaa tttgcatttt
      661 cgatttctcc aaactgatca atgtgggcgt aattcgttcc atctgtcagt gtaggattag
      721 ctgcaccagc tcgtgttgga tcaatgacag gcttgttttc agaagttgtt ggattttcat
      781 cggtaaatcc atgagctaaa tcatatgcta atttttcttt acttacgccc atttcagaaa
      841 gataaccgta aggatctgta tgatcgcccc agatgttttg tgttacccat aaatgcgatt
      901 tgattcccgg ttggttataa ggagtgtcca acgttaatgg aataccatat ttcattgctg
      961 aatctctagc caattcaacg tatgccttgt agtttttctc aaacgttgct ttatcatgtg
     1021 tgtgttgtaa ctcaatctgc acaggactgt tggcattagc atacgaacca gcaccgtact
     1081 gtacataacc aggttgaccg acttgataaa caattccgcc gtctcccaca atataagcag
     1141 tataagcgct agtccatgaa cgttgcatat actgcgcttc attgcgtcct gttgctgttt
     1201 cattagccgt ttcatgcagt aaaatatact gattatttgc tacttgtgag ctaccttcat
     1261 ttgcgcccaa attaaattca ttgttgatag tataggcaaa tccgttaatt gg
//
LOCUS       DANJMI010000148         1309 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000148 DANJMI010000000
VERSION     DANJMI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.155"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(48..347)
                     /locus_tag="PWU81_003074"
     CDS             complement(48..347)
                     /locus_tag="PWU81_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111682.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(353..583)
                     /locus_tag="PWU81_003075"
     CDS             complement(353..583)
                     /locus_tag="PWU81_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111683.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(833..1033)
                     /locus_tag="PWU81_003076"
     CDS             complement(833..1033)
                     /locus_tag="PWU81_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL1111684.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          450 a          216 c          200 g          443 t
ORIGIN      
        1 gtatggtgat aaaatccgtt ccgggctacc tttttaggta gcctacttta atctttgtat
       61 ctttctggat caacgaaagt atactttata tagtcataac gccgatgatc gcttcgagcg
      121 tctagcacat cagtcacgat atcaaacaaa aaatatacgt ctttcttcat tctagttttc
      181 gcagcaggga ttttaaagta gttcttatta gaatagtaga gattaattaa taagctatct
      241 tcgattgcta aaaagaaaac ttctgaatcc cacaccttat aaaaatcttt gacaaatcta
      301 ttcgaaggat caaatttaaa ccataattgc gtctcattaa aaagcataac cattactccg
      361 atctattttt aaacttagtc tctacctcta atatatatcg agtttttatt ttgccttcag
      421 agaatactgt ttcttttttt gcagttacag gttgtttatt ttcggaaaaa gctaatatag
      481 ctaaaattga aacatccatc tggaatttgt ctttttcgtt gctttgctca taaaaatctg
      541 catattcatc actaatattt tttctaataa attcttccat cataaagatc acctcgaaaa
      601 gagtatacga acaaacgttc tttttgtaaa ggtggaattt attatactaa ataaataggt
      661 gaacaagtac ttgtgccaaa ttatgtgcca aaaaaaatcg aatttaataa aacacaaaca
      721 aaaagaaatc ctattatgat aacatttctt ataataacaa acacaacaga agacgtgtaa
      781 tagttagtca ggaacgtaca aataacccct gtatcctttg cggtacaggg ggttattttg
      841 tataagtaga cattgtagtt tctaaactgc tatgtcctaa acgtttcgat acgctaagta
      901 tatttactcc ttgataaagt aaaatagatg catgcgtatg tcttagtcca tcaataaaca
      961 gtcgaagaga aaatagggaa aaattgatta ctaaagaaaa acttactagg tcgacggtgt
     1021 agttgatttt catacttcaa tagattcgaa ttaaacgatt tcgattggct tataccagtc
     1081 ctatccaacg aaagatatat cgttcttaac tctagcggta taacttgaag ctttacgtaa
     1141 agccaatggg aatgaactgg agatgacttt ttattatttt tagtaatagc tgatgggtta
     1201 atacttacaa ctttcctttt caaagtacca tataaaaaaa cggtggggtt gtttagcgaa
     1261 ataaaggatc taagccaata gcttagatcc tttattctat gctttatat
//
LOCUS       DANJMI010000149         1269 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000149 DANJMI010000000
VERSION     DANJMI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.156"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(60..278)
                     /locus_tag="PWU81_003077"
                     /pseudo
     CDS             complement(60..278)
                     /locus_tag="PWU81_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(544..738)
                     /locus_tag="PWU81_003078"
     CDS             complement(544..738)
                     /locus_tag="PWU81_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111685.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(838..924)
                     /locus_tag="PWU81_003079"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="PWU81_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          448 a          214 c          174 g          433 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctcggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgt
//
LOCUS       DANJMI010000150         1246 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000150 DANJMI010000000
VERSION     DANJMI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.157"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            200..742
                     /locus_tag="PWU81_003080"
     CDS             200..742
                     /locus_tag="PWU81_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111686.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="PWU81_003081"
     CDS             898..>1246
                     /locus_tag="PWU81_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDL1111687.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DANJMI010000151         1214 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000151 DANJMI010000000
VERSION     DANJMI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.158"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            70..1026
                     /locus_tag="PWU81_003082"
     CDS             70..1026
                     /locus_tag="PWU81_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111688.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(1080..>1214)
                     /locus_tag="PWU81_003083"
     CDS             complement(1080..>1214)
                     /locus_tag="PWU81_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL1111689.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          427 a          179 c          207 g          401 t
ORIGIN      
        1 ttgacaggga atcctaaaaa cattcggctt tttttatata atctttttaa ctcaacagag
       61 ataagttttg tggaattgta tacgctacct ataattataa aaacagaaaa aatgttaaaa
      121 gatttaaaac agtattatcc tctgtctgct tatgaaaaaa agaaattatt ccacttttta
      181 gcaatcaacc tgttggctga acaagctgat tctttagata ttgctagcaa tcagtcgcta
      241 tttttttcgg atataggatt ttaccactta aaaggagtat taggagaaaa atccgttatt
      301 tttatcaata agacactctc ttggctttat cttcatgata aattggatca aaaatacttg
      361 agaaaaaatg aagatgaaac aatcgaatgg ttgaatgaac gattcattga aagttttgaa
      421 aatcagttta atctgttgcg agaagagacg agccaattga tccgaagaga gttaaccaaa
      481 atacatttta aacttttgca ttacactatt aataaagctg atgaatttcc acttgatgtt
      541 tcttttttta gagaaaacta tccagcattt tattgctatt tgatagagta tataaatgat
      601 ctaacgtcaa aacgcaaaga gttgcttcaa gataaatttt ttcttttttt cagttattta
      661 ttattattga tcaatcacat acccattcaa ctgatttctg aaccagtaaa gatcatcatc
      721 gatttttctt atggtgctgc ctataatcaa tttatcaaga aaaatttaag cttgtacatt
      781 aatctgaaca cggaagtgat agaggcagga aagcctgagt tcccggatgt tataatcacg
      841 aataggaata atctatatga agaaagtgca tctcaagtag tggtttggct cgatccgcca
      901 agagcagtag actgggggaa tcttacaaag attttgttga ctatccaaga agagaaatac
      961 aaagacagca aaattgacaa gcaaatagac gattttcctg aagaaatcat cgacaggatc
     1021 gaatagtgaa cttcttttag aaagtccgac tgctttttct aaaaatatta tttgtcaaat
     1081 taagcgagac aaaactttcc atttacgtaa ctcaaaaaaa cttctaatgg tgtttggtaa
     1141 tttagtgatt ttctagggat atggtttctt ttggacgcaa cggatgagat gaatccttga
     1201 ttgacttggt tgaa
//
LOCUS       DANJMI010000152         1195 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000152 DANJMI010000000
VERSION     DANJMI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.159"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..1076
                     /locus_tag="PWU81_003084"
     CDS             <1..1076
                     /locus_tag="PWU81_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL1111690.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DANJMI010000153         1167 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000153 DANJMI010000000
VERSION     DANJMI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.160"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..969
                     /locus_tag="PWU81_003085"
     CDS             <1..969
                     /locus_tag="PWU81_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL1111691.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          395 a          207 c          232 g          333 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt accctta
//
LOCUS       DANJMI010000154         1082 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000154 DANJMI010000000
VERSION     DANJMI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.161"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            281..973
                     /locus_tag="PWU81_003086"
     CDS             281..973
                     /locus_tag="PWU81_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL1111692.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          463 a          153 c          181 g          285 t
ORIGIN      
        1 cgttttttgt aataaataca gtaatctatg aaatgaaaag ggttacgttt aataaaactt
       61 gcttttttgt ttttgttcac cataaatatt tatttaagaa agtattaacc tgcatttttc
      121 tctataaaaa agatttttta aatagataaa aaaggaggat taaatgagta tttgatagta
      181 tttatattaa aattacatat gtaatttaaa aacgcttgac attgtgtttg agatcactta
      241 cttttagcat gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac
      301 tggtgggatt gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa
      361 tgaaattatt ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca
      421 gcttgatcat acattgacag ataaagagca agcgattaca gatcttaccg ctcgactagc
      481 acaaaaagaa cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt
      541 aaatgaatta gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca
      601 attagtcagc aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga
      661 tgagatcaac caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact
      721 acaagaagca caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca
      781 aatcgcaaca ctgacacaag aaaaaacaaa tgtagaaact cagttaacag atacacaaaa
      841 gaaattagac gaagcaactg ctgaaaactc aagctggaaa caatatattc aaaagttaga
      901 aaaagcaaaa gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca
      961 tttgcgtaaa taatctttct taacaagtaa acctaaatta gtaaagaagc aacagtaatc
     1021 tatactaagt agttcagttt tggagtacaa aatgacaaaa tgtacttcaa aatggactac
     1081 tt
//
LOCUS       DANJMI010000155         1021 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000155 DANJMI010000000
VERSION     DANJMI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.162"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..460
                     /locus_tag="PWU81_003087"
     CDS             <1..460
                     /locus_tag="PWU81_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDL1111693.1"
                     /translation="LLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSV
                     IYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFM
                     KVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            469..696
                     /locus_tag="PWU81_003088"
     CDS             469..696
                     /locus_tag="PWU81_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL1111694.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          319 a          166 c          180 g          356 t
ORIGIN      
        1 attgctatta aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact
       61 tttttatact cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt
      121 atttgtttct gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt
      181 gaaactattg aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact
      241 aaaaaaaatc gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa
      301 atttatgaaa gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt
      361 cgtattcatt ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca
      421 tgcgatttta cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc
      481 gttaatttag atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa
      541 gtgggcatta cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt
      601 ttttccactc tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc
      661 tataaaaatg actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat
      721 aaaaaataat ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc
      781 agatttttta tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata
      841 caaagggggc cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt
      901 tacgttgtag ttggatctgc ttttagtata atactttatt cattccacca gactgatagc
      961 agtttgagca gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag
     1021 t
//
LOCUS       DANJMI010000156          986 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000156 DANJMI010000000
VERSION     DANJMI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.163"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..>986
                     /locus_tag="PWU81_003089"
     CDS             <1..>986
                     /locus_tag="PWU81_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL1111695.1"
                     /translation="LEPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITD
                     SEYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQL
                     DDTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTG
                     KKNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVG
                     SIAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFY
                     ENFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKM"
BASE COUNT          375 a          159 c          189 g          263 t
ORIGIN      
        1 ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat ttcaagagta
       61 ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag aataaataaa
      121 gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat gcaaagaaaa
      181 ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc agaagtgaaa
      241 aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa cttcttcaaa
      301 caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa ggaaacgaaa
      361 aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga aattcggttt
      421 cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa gaagactgga
      481 aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa agcaggttta
      541 atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa aacaaattat
      601 aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt aggatcaatt
      661 gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt tgtgaaaatg
      721 agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac atttatcaaa
      781 gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga gaatttcgct
      841 aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg cgctttcttc
      901 aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct gaaggaaacg
      961 aaaaaaggct tcttacaaaa gatgtt
//
LOCUS       DANJMI010000157          967 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000157 DANJMI010000000
VERSION     DANJMI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.164"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            142..399
                     /locus_tag="PWU81_003090"
     CDS             142..399
                     /locus_tag="PWU81_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111696.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(631..>967)
                     /locus_tag="PWU81_003091"
     CDS             complement(631..>967)
                     /locus_tag="PWU81_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HDL1111697.1"
                     /translation="VIGIGNIIKIVLSASIPILIDQASEHRSLLLIVSIASAIITIIQ
                     SGMSAFNYQDKVQTSTELLMKIEKEKLLYITKTSPYNKTDEENFHLIVTTLQTELNDI
                     ISDFNQVNN"
BASE COUNT          369 a          122 c          132 g          344 t
ORIGIN      
        1 tttctgtcca cgttcatatt actccaaagt tttcctcatt ccaaaagaaa agcacagaat
       61 acacaaagaa gcgtttttat ttgtataaac tatatcaaag ttctatactt aaattatcaa
      121 ataaaaagga cgtgaagata aatgtcaaat ttagaaaaca aagaagaaaa agttgtaaac
      181 aaaattgttt ctgttgtcaa taaactggat aaagagttag acgaacttga tacgttatct
      241 gaaaatccag aaaaaaaaca taacctaaaa aaatggttag ttgaacgaaa ggctattcat
      301 gaaattaaaa aagttcttca tgaagctgat aaatatgaaa aatatgatga aaaagaactt
      361 gataaagaat ttaaagaaat caatgacttg ttactataat tgatttatac gactcatttt
      421 tgagtcgtca gactgcaaac aacactctaa atttaggagt ttgtctacag tctgaataaa
      481 ttttaaattt attgtccata atgttattat gaatttatag cattaagaaa agagataaga
      541 ctttacattt ttttaggaat gtaaagtctt atctcttttc ttaattgtac aattagtatt
      601 atctgcacta ctttctaatt aaataaattt ttagttatta acttgattaa aatcagagat
      661 aatatcattt aattcagttt gaagggttgt aacgattaaa tggaaatttt cttcatctgt
      721 tttattatat ggggatgttt tcgttatata taacaacttt tctttttcaa ttttcattaa
      781 taactctgtc gaagtctgta ctttatcttg ataattaaat gcagacatcc cactttgtat
      841 aattgtaatt attgctgaag caattgaaac aatcaatagt aaagacctgt gttcggaagc
      901 ctgatcaatt aatattggga tactagctga taagactatt tttataatat ttccgatacc
      961 aattact
//
LOCUS       DANJMI010000158          943 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000158 DANJMI010000000
VERSION     DANJMI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.165"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..>943
                     /locus_tag="PWU81_003092"
     rRNA            <1..>943
                     /locus_tag="PWU81_003092"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DANJMI010000159          910 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000159 DANJMI010000000
VERSION     DANJMI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.166"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..>910
                     /locus_tag="PWU81_003093"
     CDS             <1..>910
                     /locus_tag="PWU81_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL1111698.1"
                     /translation="KSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQ
                     KNISTSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALL
                     DIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIV
                     QHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASF
                     KDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYT
                     TTLRTFKKFLPQIQNALHYSYSNGPLE"
BASE COUNT          275 a          184 c          182 g          269 t
ORIGIN      
        1 taagtcttgc caaagaacat tcgtagcgga tacctctgtt gctgaaaaac actgtttcat
       61 tagtcaaaaa gttcgttggt ctgtcattgc tcgcttaaaa gagaatactt ccatgacgga
      121 aatcgctcgt cagaaaaata tttccacttc ttcggtctat cgtgtcatga agcgattcta
      181 ccgcccattg aatcctttca aacagacttt gccaaaggtc ttatgctttg atgaattcaa
      241 atcggttcga agtgttgcca gtgcaatgag ttttatcatg atggacggac aaagccatgc
      301 attattggat attgtcgaaa atcgacgctt gccttattta gaacgctatt tttctcggtt
      361 ttcattagcg actcgtgaag ccgttcaatt gattgttatt gatatgtatg cgccttatgt
      421 ttcgttggtg aaaaagcttt tcccgaatgc tcagttgatc atcgatcgtt tccacatcgt
      481 ccaacatatt ggcagaactt tcttgaatca tcgagtgaaa gaaaccacgg tacgacttaa
      541 aggaaattcc cactctcaac gagggttagg gaaaaaattg aagcgctatt ggaaactcct
      601 acaaaaagaa gaagcaaagt taaactatga aaaacgggtt tggcgtgcca gttttaaaga
      661 ttatttgact gaaagtgaga ttgccgatcg actgttagct gcttgtccca acttacgcca
      721 aggctatcag ttataccaag acattcttta tgcggtaaag aaaagagatc agtcactatt
      781 tgaggattgt ttaactagag acattagcgg tctgccagaa acctatacaa ccactttacg
      841 aacgtttaaa aagtttctgc cacagatcca aaatgcgctt cactacagct attcaaacgg
      901 accattggaa
//
LOCUS       DANJMI010000160          890 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000160 DANJMI010000000
VERSION     DANJMI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.167"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..888
                     /locus_tag="PWU81_003094"
     CDS             <1..888
                     /locus_tag="PWU81_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HDL1111699.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DANJMI010000161          843 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000161 DANJMI010000000
VERSION     DANJMI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.168"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(<1..>843)
                     /locus_tag="PWU81_003095"
     rRNA            complement(<1..>843)
                     /locus_tag="PWU81_003095"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          184 a          259 c          187 g          213 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gct
//
LOCUS       DANJMI010000162          839 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000162 DANJMI010000000
VERSION     DANJMI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.169"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            76..>839
                     /locus_tag="PWU81_003096"
     CDS             76..>839
                     /locus_tag="PWU81_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111700.1"
                     /translation="MEIFGFYDTAFHINRALSLESIFSSPINFETFRSYGMQVNNFYP
                     WLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTT
                     SSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLS
                     VAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLN
                     RPYVDILQKRAIYLGKEFLIGSIDSELLSFGIGLTLL"
BASE COUNT          262 a          114 c          122 g          341 t
ORIGIN      
        1 aaaatttttt ctgtaaaaat atttatagtt tatgcatttg ttagtttatt atatttattt
       61 ccagcattta aaaacatgga aatatttggc ttttatgata cggcatttca cattaatcgt
      121 gcgttaagtt tagaatctat ttttagctcc cctattaatt ttgaaacgtt tagatcatat
      181 ggcatgcaag taaataattt ttacccatgg ttaactctat atcctttatt tttattgatc
      241 aagtttacta atttagcaat aggctataac ctttttcttt atattgttac gctcattact
      301 ctatttatat gtcattatgt aatgtatgaa attaccaaaa aacatgttac tagctccttt
      361 ttcgctatta tatatacaac atcttctttt aggagtgtag aaatatttct tcgaggggca
      421 atgggggaat tactcgcaat gtcaatactt cctcttattt tactcgggtt tataaagctt
      481 tacgactcaa aaaaagaatc ttgggttatg ttggcgatta gcatgacttt attaatttac
      541 actcatgttc tttctgtagc tatgactatg gtaatgatta taattgcttt aattattcaa
      601 ttttatcgga aaaagaaaat agaaatcttc ttaattatta aattaataaa agctgctata
      661 gttactttac ttctttcact agcgtttata ggtcctatga ttgagcagac attgtatcaa
      721 gatctgaata gaccttatgt ggatatatta caaaaaagag caatttatct gggtaaagag
      781 tttttgattg gaagtataga ttcagaatta ctatcatttg gtattggctt aactttgct
//
LOCUS       DANJMI010000163          761 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000163 DANJMI010000000
VERSION     DANJMI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.172"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..519
                     /locus_tag="PWU81_003097"
     CDS             <1..519
                     /locus_tag="PWU81_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL1111701.1"
                     /translation="ISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          343 a           95 c          105 g          218 t
ORIGIN      
        1 attagtattt taaattcaca attgtcttca aacaaccaaa ctcttgaaca actaagaact
       61 aatattgaag tttccaaaca aaaagtggca tctttagaaa acgacaagaa tcaattagta
      121 tcagagaaaa ctaatttaga aaatcaattg tcagcaaaag actctgaatt gcaaggaaaa
      181 caaaatgaaa ttaatgcgaa aatagacgaa attaaccaaa aagtagctga aataaatcga
      241 ttaaatagcg attggaataa tcgtttacaa aatgaaaaga acaaatttaa tgaagaacaa
      301 tctactatta atagtttaaa ccagcaaatt aatcaaatgt tgagtgaaaa aaataacatt
      361 caaaatcaac tgcaagctac tcagcaaaag ctagaagaag aaaagtcaaa aaatagtagt
      421 ttgaataatt acattgaaaa gcttcaaaaa gctaaaaatg atgtagagga taccgcaaat
      481 aaagcacaac aaattgtaaa tgaacataca atccaataga tatagtttta atttaacaaa
      541 tggtatgttt ggagataatg agatagaata taaatgtcat aatattatac aatctaaaca
      601 ccgtttcctt ttctattttt gatgacatca gtatatagct actcgaaaga gtagtctttt
      661 tattattctc gcttattatt gatatgaaaa tttcgatttg acaaaaggta ccgaataatt
      721 ttgtaatatg aattattatc aagaagtaat tatgatgata a
//
LOCUS       DANJMI010000164          696 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000164 DANJMI010000000
VERSION     DANJMI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.173"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(54..629)
                     /locus_tag="PWU81_003098"
     CDS             complement(54..629)
                     /locus_tag="PWU81_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL1111702.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          124 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaac
//
LOCUS       DANJMI010000165          638 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000165 DANJMI010000000
VERSION     DANJMI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.174"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
BASE COUNT          221 a           98 c          103 g          216 t
ORIGIN      
        1 gaagagtatt atacaagcta aagcttttct tcattgccac tcaaatgagt ggctttttta
       61 tgtatccttt tatggattaa tgaaaggatg tttcacatag ttatacttct gtatatttga
      121 aaagttttac tttgattttt aaatagaaag acatttgggt taaattgtga gataataata
      181 aagaagagtt taaagcgcac cccaaaccac ttccccataa gtgtgttacg ctttaaactc
      241 ttttatattt gaagccatta aaaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttacaattg gagtggtagt taattagtga cttatttttg attttatagc actgatacta
      361 taaaatatag atatcatcat attacacaat cttaatacta acttaaaaaa tatctccttt
      421 cacaagtatg gtgataaaat tcgttccggg ctaccttttt aggtagccta ctttaatctt
      481 tatacctttc tggatcaacg aaagtatact ttatatagtc ataacgccga tgatcgctac
      541 gtgcgtccgg cacgtcagtc atgaatcggc tacactagac tagacagaaa aaataaggtg
      601 tgtagaataa taaaaaacac actggaggat ttttcatg
//
LOCUS       DANJMI010000166          534 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000166 DANJMI010000000
VERSION     DANJMI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.175"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..>534
                     /locus_tag="PWU81_003099"
     CDS             <1..>534
                     /locus_tag="PWU81_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL1111703.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DANJMI010000167          522 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000167 DANJMI010000000
VERSION     DANJMI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.176"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
BASE COUNT          174 a           60 c           67 g          221 t
ORIGIN      
        1 tggatatgtt acaatcctta gcttgctcct attctctatt ggacaaagtt atatcagttc
       61 aatatttata aatacgaagt atgatgcata ttttgataga aacagtacat cgatttctca
      121 cgtagtgatg tttgctctct ttttttcagc aaatcttttt ttagttcata aattatcaaa
      181 aatgaatatt tacgaaaaaa aagttatcag cttaagctca tatatatttt ctatcaatat
      241 gttagcttct actagtatag ttttcttact tataagtaat aattttatta gaattattca
      301 aaatatattt gtattaaatt atattattta tgccagtgta ttaatgagta attatatgag
      361 aacaaaagta aagaattata tctacttgct tgcgtatttc ttttttatca ttgtgtgttt
      421 gatattcatc aatataagag gaactgcttt tgtaaatgga atcctaccat tatttagaga
      481 aaatattatt tttaaggctg tttactattt ttttgagtaa ga
//
LOCUS       DANJMI010000168          513 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000168 DANJMI010000000
VERSION     DANJMI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.177"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..>513
                     /locus_tag="PWU81_003100"
     CDS             <1..>513
                     /locus_tag="PWU81_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1111704.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSS"
BASE COUNT          203 a           68 c           89 g          153 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agt
//
LOCUS       DANJMI010000169          487 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000169 DANJMI010000000
VERSION     DANJMI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.178"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(<1..>487)
                     /locus_tag="PWU81_003101"
     CDS             complement(<1..>487)
                     /locus_tag="PWU81_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDL1111705.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DANJMI010000170          468 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000170 DANJMI010000000
VERSION     DANJMI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.179"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..>468
                     /locus_tag="PWU81_003102"
     CDS             <1..>468
                     /locus_tag="PWU81_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL1111706.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DANJMI010000171          462 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000171 DANJMI010000000
VERSION     DANJMI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.180"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(<1..430)
                     /locus_tag="PWU81_003103"
     CDS             complement(<1..430)
                     /locus_tag="PWU81_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL1111707.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DANJMI010000172          451 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000172 DANJMI010000000
VERSION     DANJMI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.181"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..>451
                     /locus_tag="PWU81_003104"
     CDS             <1..>451
                     /locus_tag="PWU81_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1111708.1"
                     /translation="FRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVR
                     TLNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNE
                     LLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          147 a           89 c           87 g          128 t
ORIGIN      
        1 tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa atgtatgggc
       61 atcactacac gccccaaacc atgtccaaca tgactaaagt gctgactgaa gaagtaaatg
      121 catttaaagt tagaactcta aatgataagt atgtcgctat tttcatggat gctacttata
      181 ttccattaaa acggcaaacc gtctccaaag aagcaatcta tattgccatt ggtatacgag
      241 aagacggcac taaagaagta ctaagttatg cgattgctcc gactgaatcg acctacgttt
      301 ggaatgagtt gttacaggac atttactcca gaggggttca tgatgtctta ctgtttatta
      361 ccgatggctt aaaaggcatg aaagatacga ttcaccagat ttatcctaaa gctaaatacc
      421 aacattgctg tgttcacgtt tcccgtaata t
//
LOCUS       DANJMI010000173          432 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000173 DANJMI010000000
VERSION     DANJMI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.182"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(<1..132)
                     /locus_tag="PWU81_003105"
     CDS             complement(<1..132)
                     /locus_tag="PWU81_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL1111709.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          150 a           85 c           83 g          114 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aa
//
LOCUS       DANJMI010000174          415 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000174 DANJMI010000000
VERSION     DANJMI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.184"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(<1..339)
                     /locus_tag="PWU81_003106"
     CDS             complement(<1..339)
                     /locus_tag="PWU81_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1111710.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DANJMI010000175          414 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000175 DANJMI010000000
VERSION     DANJMI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.185"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(109..>414)
                     /locus_tag="PWU81_003107"
     CDS             complement(109..>414)
                     /locus_tag="PWU81_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1111711.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          116 a           96 c           82 g          120 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggt
//
LOCUS       DANJMI010000176          404 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000176 DANJMI010000000
VERSION     DANJMI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.187"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..>404
                     /locus_tag="PWU81_003108"
     CDS             <1..>404
                     /locus_tag="PWU81_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111712.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DANJMI010000177          336 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000177 DANJMI010000000
VERSION     DANJMI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.188"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(<1..>336)
                     /locus_tag="PWU81_003109"
     CDS             complement(<1..>336)
                     /locus_tag="PWU81_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HDL1111713.1"
                     /translation="KKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPIEEQAIN
                     FAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTIK
                     YIYTVKKNT"
BASE COUNT          112 a           40 c           58 g          126 t
ORIGIN      
        1 tttgtatttt ttttgacggt ataaatatat ttaatcgtat tatcagcgtc tgaaaatact
       61 ggaaagaaaa tagaactatt tgccgtattt tctccatcga gcattttaaa tggcgaacca
      121 agagtatagt taatatcagt ttcggtgtta ttttccatta aaagaattga agttatggag
      181 tgttcaaaat cagttttcgc aaaattaatt gcttgttctt caattggttg ggagttgata
      241 ttgaaattag aattttctga agcataggta tttgtaattc ctatactaaa aattccaatc
      301 atcaaggaaa agaggagacc tagataaatt ttcttt
//
LOCUS       DANJMI010000178          321 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000178 DANJMI010000000
VERSION     DANJMI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.189"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(69..321)
                     /locus_tag="PWU81_003110"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="PWU81_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DANJMI010000179          309 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000179 DANJMI010000000
VERSION     DANJMI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.190"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            1..309
                     /locus_tag="PWU81_003111"
                     /pseudo
     CDS             1..309
                     /locus_tag="PWU81_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DANJMI010000180          282 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000180 DANJMI010000000
VERSION     DANJMI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.191"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..>282
                     /locus_tag="PWU81_003112"
     CDS             <1..>282
                     /locus_tag="PWU81_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HDL1111714.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DANJMI010000181          274 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000181 DANJMI010000000
VERSION     DANJMI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.192"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            46..>274
                     /locus_tag="PWU81_003113"
     CDS             46..>274
                     /locus_tag="PWU81_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL1111715.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVS"
BASE COUNT           84 a           51 c           52 g           87 t
ORIGIN      
        1 atcctttaga attaatttgg ctaccaattc ataggaggat tcaagatgtc tcatactcag
       61 cttatcaaag ataccctaaa tattctagac ctaaatatct atttcgagga aaattgttta
      121 actaaagaaa aatacaaagg gcaaatttgc ttgatttaca aaggaactct tctgtacaag
      181 ccagaagagt gttcccattg tctttgtgtc gtcccctcta ggatcattcg ttgggggacg
      241 acaaccgttc ggttgttact gaatgatgtg tctg
//
LOCUS       DANJMI010000182          266 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000182 DANJMI010000000
VERSION     DANJMI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.denovo.193"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(36..>266)
                     /locus_tag="PWU81_003114"
     CDS             complement(36..>266)
                     /locus_tag="PWU81_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL1111716.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           78 a           39 c           47 g          102 t
ORIGIN      
        1 atagaaggac tgaccttctg tctcagattt ttttgttatt tcacttgaat caattgattg
       61 atcaaaaaaa tcctgatttt catgttctca aatgatttaa atccataaga taatcgtttc
      121 agtgtcttga tatggggaat cttggcctct attttgccat tagaatacgg gtaaatcata
      181 gcattgtaaa taccttcttc atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca
      241 tcaagtgttt caggaagatt ttttaa
//
LOCUS       DANJMI010000183        10375 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.guided.082, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000183 DANJMI010000000
VERSION     DANJMI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.guided.082"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..871
                     /locus_tag="PWU81_003115"
     CDS             <1..871
                     /locus_tag="PWU81_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL1111717.1"
                     /translation="AKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQLANYQLIVF
                     LDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEIYHFDFLEAK
                     ELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKRDVTTNYFIL
                     PHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQKKLVTDNQAI
                     AHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKRFITNDSTIY
                     EQYEYDLSSVENK"
     gene            complement(1021..1962)
                     /locus_tag="PWU81_003116"
     CDS             complement(1021..1962)
                     /locus_tag="PWU81_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL1111718.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2088..3209
                     /locus_tag="PWU81_003117"
     CDS             2088..3209
                     /locus_tag="PWU81_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL1111719.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3467..4969
                     /gene="eat(A)"
                     /locus_tag="PWU81_003118"
     CDS             3467..4969
                     /gene="eat(A)"
                     /locus_tag="PWU81_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL1111720.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            4997..5563
                     /locus_tag="PWU81_003119"
     CDS             4997..5563
                     /locus_tag="PWU81_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL1111721.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            5774..6835
                     /locus_tag="PWU81_003120"
     CDS             5774..6835
                     /locus_tag="PWU81_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL1111722.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6838..7734
                     /gene="murQ"
                     /locus_tag="PWU81_003121"
     CDS             6838..7734
                     /gene="murQ"
                     /locus_tag="PWU81_003121"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL1111723.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7748..9730
                     /locus_tag="PWU81_003122"
     CDS             7748..9730
                     /locus_tag="PWU81_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL1111724.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9795..10241)
                     /gene="nrdI"
                     /locus_tag="PWU81_003123"
     CDS             complement(9795..10241)
                     /gene="nrdI"
                     /locus_tag="PWU81_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL1111725.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3375 a         1837 c         2250 g         2913 t
ORIGIN      
        1 tgcaaaaaat aaatcacttt attttttgcc ttccatccgc tctttagatg tttggtccgg
       61 aaaagattta ctacagtcgg aattcattaa atcagctaga caacagttgg caaattatca
      121 gctgatcgtt tttttagatt atggatttga tatggagatg gctcgacgcg tgcgcccatt
      181 taccaatgca aaaattgtgt tatttttttg gaatcacttc aaagcagaac acgaggcgat
      241 gctgaagcag gcacaagatg aaccggcaat cgatgagatt tatcattttg attttttgga
      301 agcaaaagaa cttgggctga aacataatag ttcattttat tcgaaacata tgaatttttc
      361 ttctagcgaa ccaaagacgg atctattttt tggtgcaacg aataatggga gaaaagaacg
      421 agcagaaagc tataagcagg aatttataaa aagagatgtt acgaccaact atttcattct
      481 tccacatcga gggaatgaac aacctggata tcttacttat gaagagtacc tgaaaaaaac
      541 aggggaatcg cgcgggattc tggaactgtt gcgggagggt cagcaaggag tgactttgcg
      601 gacatttgaa tccatgtatt ttcaaaagaa actagtgacg gataatcaag cgattgctca
      661 ttatcatttt tatcgtcctg aaaatatttt tttgcttcga gaacgtgatt tagatgaatt
      721 accggaattt ctgcatacac catttaagcc aatcctatca gagctattgg aattttatga
      781 tgcagaaaat tgggcaaaac gatttatcac taacgattcc actatttatg agcaatacga
      841 atatgacctc tcctctgttg aaaacaaata aaaaacctac attttgaaga gattatgtac
      901 tgaaacaaac aagcacaatt cttatggcat catgagagtt gtgttttctt tttgatcttt
      961 tattgggctt aaaaaaagag aaccagctgc tgattcagct gattctcatc agagagcctt
     1021 ttattctgcg ccaaggattc cttcttttgc atggactaat tgggtcaaaa aggcacaata
     1081 tggtgtagca atatcatatt tttgtccttt tcgccaaatg gcaccattga tgtagtcgat
     1141 ttcagtcaaa cgatggtttt tgatcaggtc ttggtacata gatgggtaat gcaacccgat
     1201 gttgtcagga tcataagtag aagcaatatg ttggtacact tcttcttgat ctagtatgat
     1261 tccttctttt gctgcgatag aagcaaattc agatacgatt gttcgaacca tgctctctgc
     1321 cgctttttgc gcgcctagtt cagcaatatt acaatccaaa atcgtgcata aaccattcaa
     1381 tgttccattt acacaagctt ttcgccaaat ggaataacga acgttatggc tatagatcgg
     1441 atttaaattt gcttcttgaa agacttctac aacttttttt gtaaatgctt cgccttctgg
     1501 ttccaagttc tctagttcga tttctccatc acctaacaat gtgacttttc cgggaccagc
     1561 tagaccagct gtccacatcg tgatcccaaa taagatattt ttctttggca catatttttc
     1621 cagtacatct tcatgaccta atccatttag taaacacaaa acataagtat tgtcagtaat
     1681 aatggattgg atcgaacaaa acatatcatc taattgattg gcttttgtta atgctacgat
     1741 caagtccacg tgttcgttgc tttcgatgat ttcttctggt gaatagattg gcagttttgc
     1801 gaccacttct ttaccgttaa agtcagcaat caacccattt gtacgaatgg cttcaatgtg
     1861 agcaggccaa cgatcgatca agaggacctc atttccgctt tgatgtagca tcaatccgat
     1921 tcggctgccc attgctccgg ccccagctat tgcgattttc atgttttttc tctcctttat
     1981 tcaatgagtt cccttttagt atatagaaaa atggaagaaa atagcattgt tttgtttgaa
     2041 aaaaatgaaa aaaagtgtct tacttaagaa gaaagtgagg gatacagatg gatattttgg
     2101 cggcaattga ttcatttaaa ggaagtgctt cttcatttga actaaatcag gcagttctaa
     2161 atggctggga agatcgtaaa ggaagaaaaa tcaatgtgcc gattgcagat ggcggagaaa
     2221 atactgcaga agcaatctat tacgcgctca ggggaacatg gcgtaccatt gatgggatgg
     2281 atctcttatt ccgtcagcga aaaatcagct atttattgac aacttggcaa ggaaaaaaca
     2341 ccgcagtcat cgaatctgca caagcattgg ggttagatct acttgataaa ccaacggatc
     2401 aaactatccg acttgcttcc agttatgcct taggagaact tattaaagat gcactgcttc
     2461 aagaagtaga acaaattatt gtcactttag gaggaagcgg gtgtagcgat ggtggtctag
     2521 gattactcca atccttgggt gcttctttag aagggtacac agaaggaaat cctttattat
     2581 ctactaaaaa acttcatttt gatcgaagaa acgttcttgt ttctaaagta aaattgatta
     2641 tcgctgcaga tgttaccaat ccatatacag gtcaacaagg agctgcttgg caatttggaa
     2701 gacaaaaagg aggaatagat acgacgctag ctttcttaga taaacaagca ggaaagattg
     2761 ctcagcaaat aaaagatttc tgggaggtgg atttagatga tattccagga agtggagcag
     2821 caggcggtat tggtggagcc ctttttttgt tgggagcgaa gatggtttct ggtttcgatt
     2881 tagtcagcca aatcactgga ttggaaaaaa agatcgaaca agcagatgtc atcattacag
     2941 gagaagggcg gatcgatcag caaacgatcc acggaaaagt tccatacggt gtagcagtgc
     3001 ttgcaaaaaa atatcgaaaa ccaataatcg ctatttgcgg aagtagagac caagagctag
     3061 aaggactttc tgattttctc ccaattgtct taagtattca tttgggtcca ataggtttag
     3121 aagaagcaat ggaacacagg caaacattag aaaaaacaaa aatcttagga cagacactta
     3181 gcagattttt caccatcctc ccgaagtagt tgacaaaatc attttcttta tgctatcgta
     3241 atttcaaata taggacaaag tgagggatcc gtgcattgaa aaattaagtc gattgatgaa
     3301 agctgcattc agactgtacc ttgtggagac aaggaagcag tgtgcagatt ttcgggattg
     3361 gcttggtttt ttcgatgtat gggtcttttt gtgtgtatcc aacccttgca tcctctttag
     3421 ccagttctga aaataggact ggtctttttt tacgaggtga tggaaaatgt ctaaaatcga
     3481 aataaaaaat ctgacattcg gctacgacag ccaaggcaca ttattatttg aacaagcaaa
     3541 tctaaatttt gacacacaat ggaaactagg acttatcgga cgaaacggtc gaggaaagac
     3601 aactttactg aatattctac aaaacaaact accttatcaa gggcaagtaa tccatcagca
     3661 agaatttgcc tatttcccgc aacagacaaa agataaagaa cgtttaacct attacgtgtt
     3721 aaatgatatt acggattttg agatatggga aatcgaaaga gagctccaat tgatgcaaac
     3781 agatcctgaa atcttatgga gagaattcag cacactatcg gggggagaga agacaaaagt
     3841 cctactggca cttttatttg tggatgacac tcatttcccg ttaatcgatg aaccaacgaa
     3901 tcatttggat atctctggta gaaaacaagt agcggcttat ttgaaaaaga aaaaacaagg
     3961 cttcatcgtg gtcagccatg accggggatt tatcgatgaa gtagtggacc atgttttagc
     4021 aatcgaaaaa agtcaactgg aactttatca agggaatttc tctatctatg aagaacagaa
     4081 aaaacttcgt gatgaatttg aaatggctca aaatgaaaaa ttgaaaaaag aagtcagtag
     4141 gctaaagaaa acagcagctg aaaaagccga atggtctcgt tcccgagaag gagataaaac
     4201 aaagaaacaa gtcggattca tcgatactga atctagacga gtgaataaag gagcagtggg
     4261 tgctgatgct gcacggacga tgaaacgatc caaagcaatc gtgaatcgga tggagaccca
     4321 gatcagcgag aaagaaaaac tattaaaaga tatcgaatat atcgattctt tgacgatgaa
     4381 tagccaagcg tctcaccata agcgactttt aagcgtagaa gatcttcaat tagggtatga
     4441 aaatctgtta ttcgagccaa ttcattttac aatcgagcct catcagcggg tggcgatttc
     4501 aggtcctaac ggtgcaggaa agtcatccat tatccattat cttctggggg cattcaacgg
     4561 caaggttata ggagaaaaat accagccaaa acatctgagc attagttatg caagccaaaa
     4621 ttatgaagac aatcgaggaa cgttggcgga atttgcagag aaaaaccaag tagactacca
     4681 agcatttttg aacaacctcc gaaagcttgg gatggaaaga gatgtttttc ataacaagat
     4741 cgagcagatg agtatgggcc aacggaaaaa agtggaattg gctaaatctt tatcacagcc
     4801 agctgaacta tatacatggg atgaaccatt gaattatttg gatgtcttca atcaagaaca
     4861 attagaacaa ctgatcttga acgtgaaacc tgccatgtta ctagtggaac atgatcaaac
     4921 cttcctggat aaagtatcta ctgagattat ttctcttgag agaatctaac ccttgtaatc
     4981 tcaagaacaa caaggtatgg ttaaggggaa ctttgccaac gaaggagaga atcacatgct
     5041 ttatgtaacg agacacggag aaacaacttg gaacgcacaa ggattagttt gcggacgggc
     5101 agatgtgccg ctgactgaaa aaggacagat gcaggcacag aaactagcag aaaaggtagt
     5161 agatttgcct gttccaatca cgaaaatcat ccattcccct cttcaacgag caagagatac
     5221 agcacaagca gttgctgatc gtctcagctt gccgctgacc gttgatgaac gattggtaga
     5281 aatggatttt ggggattatg atggactgcc aagcaaagat gagaactttc aaaaagcacg
     5341 tttagcgttc gctgtacgat tcccaaatgg ggagtcagtc ttagatgtgt atgcacggat
     5401 cgttccttta ttgaaagaat gcatcgaaga cgaagaaaat gtctatctgt tagtttgcca
     5461 taatgccttg ataagagtga tcaatgctta ttttcatccg atgcctaatg aggggttctt
     5521 tacattcatg gtagataata cagaattggt ttcttatgag taattatgtt tttgtgttat
     5581 ttaaataaaa tatttcaagg cactgctttt gcttagcagt gcctttttta ggttatgaca
     5641 attcttttga taatataaac tcaaccttta gaaatgaaat ttaattattt taataaaagt
     5701 attgaaattt tgtttcttta gatatatact ctagtcatag aaatgaagac ggatacagaa
     5761 aaagagggat aatatgtttg gcttttcaat atttatgaac gattctttgt cagaagagaa
     5821 gaaacagtat atccgccaaa tggcatccaa tggctttgtc ggtattttca cttccatgca
     5881 tataccagaa gatgatgttt cggcatatca aaagcgattg atcgaactag gaagcgaagc
     5941 gaaaaagaat aaactagaat tgatggtcga tatttcagga gaggcattgg aacgagcggg
     6001 tttttcatta gaagatctaa cgcctttgaa agcaattggt gtaacaggtt taagaatgga
     6061 ttatcatatt tccaatcagc agattgccga ctggtcacat caattgaaaa tcagtttgaa
     6121 tgctagcacg atcacgccta aagatatcga tgaattgaaa gaagcagaag ctgatttctc
     6181 acagattgaa gcttggcata attattatcc acgtcccgaa acagggctgg acaaagaatg
     6241 gtatcagaaa aaaaatcaat ggctcaaaat tcaaggtctc cttgtccaag gttttgtacc
     6301 aggagataca gagttgagag gcccactata tcaaggattg ccaactttgg aagaacatcg
     6361 agggatccat cctcttgcag gcgctctaga tctattagca agtaacacgg atattgttta
     6421 catcggagat gcaggtctgt cagaaaacgt tcaggagcaa tttgcttctt tccaaaaaga
     6481 acagacagtc ttgctccata cggaacctgt agatgaagaa ttttacgaat atatattagg
     6541 gaagcatacg aatcggcaag acgatgcaag agatgtcata cgaagcgtag atgcacgttt
     6601 tcgagagatt ccgcctatcc cagcaagaaa tacagctacg cgtatgaaag ggtctattac
     6661 tttagataac gaaaagtatt tgcgctatat gggtgagatc caattaacca aatatgattt
     6721 gccagcggat gaaaaagtga atgtagtagc taaagttatc aaagaagagc taccattgat
     6781 caaccaaatc aaagctggta tgaactatca atttataaga aaagaaggaa gataaaaatg
     6841 agcaaaatca atttagagaa cctaacaacg gaacgcagaa accaagaaac ttttggatta
     6901 gatgagatga gtatttctca agccgttcaa ctaatgaaca aagaagatca gaaagtggcc
     6961 gctgcggttg aaaaagaact gacaagtatc gaaccagtga ttgctaacac gattgagtcc
     7021 tttaaaaaag gtggacgtct gatttatatg ggagccggaa caagtggaag actaggtgtg
     7081 ttagatgctg ctgaatgcgt accgacattc ggtgtagaac cggaaatggt cataggtcta
     7141 attgctggtg gagaagaagc aatgactgta gctgtcgaag gggccgaaga ttcttatgag
     7201 ctagggcaag aagatctaat caagctgaat ttgacaaaaa atgatatagt tattggtatc
     7261 gcagctagcg gtcgtacacc atatgtgatc ggtggattag attacgcaaa atcgatcggt
     7321 gctgtcacag gaacgatttc ttgcaataaa caagccgaaa tcagtaaaca cgcggatttc
     7381 ccaatcgaag tcgatgcagg tccagaattt ttgacaggct ctactcgatt gaagtcagga
     7441 acagcacaaa aacttatctt gaatatgatt tctactatca gtatgattgg tataggtaaa
     7501 gtattcaata acttgatggt cgatgtcaaa ccaacaaatg aaaaactaat cgaacgctca
     7561 aaacgaatca tcatgcaagc gacagaagct gattacgaaa cagctgaaaa gtactttgaa
     7621 gaagcagatc aaaatgttaa gctggctatc gtcatgattt tgacaaatag tgataaagag
     7681 gaagctcaag caaaattagc tgaagcaaac ggatttgtaa aagggacctt atgaggggga
     7741 aaagaccatg gcaaaaaacg aactgacttt ggaagaactg gcaaaacaaa tttataaagg
     7801 ggcaggcggc atgggcaatg tagaaagcat tgtccactgc atgactcgtg tgcgtatgaa
     7861 gatcatcaat gatgatcttg taaatgtctc tgaattaaaa gcgattccag gagtgcttgg
     7921 tgtagtagaa gatgaacagc ttcaagtcat catcggacct gggaaagtga acaaagtagc
     7981 acagcaaatg gttgatttgg caggcgtgaa attaggagaa aaactttctt ctgtaagtac
     8041 agctacagca aattcatcag catctggaaa agaccgtgta aacgaacggg cacaagaaat
     8101 gaaagcggca caaaaatcga aacaaaaacc atctaaaatc aaatcggtat taaaagacat
     8161 ttctaatatc tttgtgccaa tgattcctgc atttgtcggt actgggatcg tggcagggat
     8221 tgccgcagtt ttagcaaact tagtcacagc cggaacactt gatgcagcaa catggcaaca
     8281 atatatcgat gtgatgaaca tcttgaaaaa cgggatgttt agctacctag ttatctatac
     8341 aggtatcaat gctgcacaag tttttggtgc aacaccaact cttggtggag ttatcggggc
     8401 cgttgtgatg ctgacaggga tgaatccgga agcaccactg aaaaacttat ttactggtga
     8461 agctcttgca gctggacaag gtgggatcct aggtgtcatc ttcgctgttt ggctattgtc
     8521 aatcgttgag aaaaaactgc ataaagtagt acctgatgcg gtagatatca tcgttacacc
     8581 aactctttca ttgattgcca tcggtctgat tgaaattttc ttcatcatgc ctttagcagg
     8641 tttcatttct gatggcatgg tcggtgggat caactgggta cttggcgttg gtggagcatt
     8701 tgccggcttc gttctaggca cattattctt gccgatggtt atgttcggac tgcatcaaat
     8761 tttgactcca atccatgtgc aaatgattga tgaaactggt cgtacgctat tgcttccaat
     8821 tcttgcgatg gctggtgcag gacaagtggg tgcagcattg gcattatgga ttcgctgcaa
     8881 aaaagataaa gaattaacag aaatgatcaa aggagcttta ccagtcggga tcctaggaat
     8941 cggcgaacca ttgatttacg gagtaacatt acctctagga cgcccattta ttactgcttg
     9001 tatcggtgga ggtatcggcg gggcagtgat cggaatgatc ggaaatgtcg gggctatcgc
     9061 tattggacct tcaggagccg ctttgatccc attgatctct gacggtaaat ggttaggcta
     9121 catccttgga ttattggcag cttatgctgg tggcttcgtt gctacattct tcttcggtat
     9181 tcctaaagac cgtttagaaa aagaagaatt agcgactgaa acagccacag taaatgaagt
     9241 gcctgaaaaa gtagtacctc aaacttcagc tgaaagtgtg actatcctct catcagttgc
     9301 tgacggaaca gttgtttctt tagataaagc aagtgatcca gtttttgcac aaaagatgat
     9361 gggtgaaggc tactttgtag aaccagaaaa cggacatatc tattctccag ttactggggt
     9421 agtcagctct gtcttcccaa cgaaacacgc tattgggatc acaacgccaa atggtttaga
     9481 aatcttgctt catatgggaa tcaacacagt tgacttaggc ggtacgccat ttgatgtgaa
     9541 agtaacagaa gggcagcaag tgacacctga tacgcttgta gcagaagctg acttagaagc
     9601 gatcaaagca gcaggcaaag aaacatccat gatcgtcttg gtaaccaata tggatcgtgt
     9661 aaaaaacttt gtcttggaaa aaacaggtaa agcaaaagca aaagcacctg tgatggatgt
     9721 caaagcttaa tatgatagaa acaatagaaa cgaaagaaga aactgaaatc tatttttcag
     9781 cttcttcttt tttattattt attgttttct gctaaaatct gataaattcg tgtcacatca
     9841 gatggtgttc cccgtaattc ataatctgcc aagaaaggaa agccaaatgt ttgtgcatat
     9901 tgtttggctg ttagacaata ctgattattg aagtttttat ttccgctacc gactacacca
     9961 agacaaagtt tatgattgtc ttcatgttcc agatattcac gcatcgtttc ggtcaagatt
    10021 tctgtatcac cgttatctaa accatttccg ccgtctaaat aagtaggaac gaaagtaaaa
    10081 aatggttctg tttctttcgt aaaatcacta ttttcatgga tttcttttaa agtaacttca
    10141 ggattgacag gatcttctgc atgcattttt tcagcatatt ctgctagatg tttggcaaag
    10201 gcacgcgtat ttcctgaaat cgatatatat aaaatgttca tggatcatcc tccaattctt
    10261 tccttttagt atagcatctt ttttaagttt tttggtggtg taatttggaa agggactagg
    10321 cgctttgctt gtataatgag aaaaaagccg gtagaattat atttctatta aagtt
//
LOCUS       DANJMI010000184        10349 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.guided.083, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000184 DANJMI010000000
VERSION     DANJMI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.guided.083"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..1045
                     /locus_tag="PWU81_003124"
     CDS             <1..1045
                     /locus_tag="PWU81_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111726.1"
                     /translation="AVRYYPINEGAARRAKEAMSFSDYRPGSATAEYRHYVDQAVEIA
                     ERQKKRVEPEYHEKIDSLLDTYARKLAENMNKGYEITARVPSVLIAGPSNFPVRAKEK
                     QNAASDRNMEEFQYIQGLLDKIRSTGMGGISADDPQAVSKLEKKLEKLEASQELMKAV
                     NAYYRKHGTLDGCPHLIERGIENLKADMASGWHYEKKPFQSWQLSNNNAEIRRLKGRI
                     EELTRQKEAAYVGWEFDGGTVEINRVANRLQIFFEGKPDAAVRDELKSNGFRWSPKAG
                     AWQRQLNDTTIRVADRIKCIQPLSGEKPSALQLAARREKEKPSIRAQLNAAKTEQAKQ
                     PPAREKSKGLEVE"
     gene            1046..1990
                     /locus_tag="PWU81_003125"
     CDS             1046..1990
                     /locus_tag="PWU81_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111727.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            1994..2197
                     /locus_tag="PWU81_003126"
     CDS             1994..2197
                     /locus_tag="PWU81_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HDL1111728.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(2497..2733)
                     /locus_tag="PWU81_003127"
     CDS             complement(2497..2733)
                     /locus_tag="PWU81_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1111729.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(2916..3302)
                     /locus_tag="PWU81_003128"
     CDS             complement(2916..3302)
                     /locus_tag="PWU81_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HDL1111730.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(3284..4612)
                     /locus_tag="PWU81_003129"
     CDS             complement(3284..4612)
                     /locus_tag="PWU81_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL1111731.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(4573..4902)
                     /gene="mobC"
                     /locus_tag="PWU81_003130"
     CDS             complement(4573..4902)
                     /gene="mobC"
                     /locus_tag="PWU81_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDL1111732.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(5160..5530)
                     /locus_tag="PWU81_003131"
                     /pseudo
     CDS             complement(5160..5530)
                     /locus_tag="PWU81_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            5675..5860
                     /locus_tag="PWU81_003132"
     CDS             5675..5860
                     /locus_tag="PWU81_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL1111733.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            6018..6185
                     /locus_tag="PWU81_003133"
     CDS             6018..6185
                     /locus_tag="PWU81_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111734.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            6803..7462
                     /gene="vanR"
                     /locus_tag="PWU81_003134"
     CDS             6803..7462
                     /gene="vanR"
                     /locus_tag="PWU81_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HDL1111735.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            7465..8826
                     /gene="vanS"
                     /locus_tag="PWU81_003135"
     CDS             7465..8826
                     /gene="vanS"
                     /locus_tag="PWU81_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL1111736.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(8917..10218)
                     /locus_tag="PWU81_003136"
     CDS             complement(8917..10218)
                     /locus_tag="PWU81_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL1111737.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         2633 a         2463 c         2611 g         2642 t
ORIGIN      
        1 ggctgtccgc tattatccca tcaatgaggg agccgcccgc cgcgccaagg aagccatgag
       61 cttttctgac taccggcccg gcagcgccac ggcggaatac cggcactatg tcgatcaggc
      121 cgtggaaatt gcggagcgcc agaaaaagcg tgtggagcca gagtatcacg aaaagataga
      181 cagcctcctt gacacctacg cccgcaagct ggccgagaac atgaataagg gctatgagat
      241 taccgcccgc gtcccctctg tcctgattgc cgggccgtcc aatttcccgg tacgggcgaa
      301 agaaaagcag aacgccgcca gtgaccgcaa catggaggaa tttcagtaca ttcagggctt
      361 gcttgacaaa atccgcagca ccggcatggg tggtatcagc gccgacgatc cgcaggccgt
      421 ttccaagctg gaaaagaaac tggaaaagct ggaagcgtcg caggagttga tgaaggccgt
      481 caatgcctac taccgcaagc atgggacgct ggacggatgc ccccatctga tcgaaagagg
      541 cattgaaaac ctgaaagcgg atatggcgtc gggctggcac tatgagaaaa aaccgttcca
      601 gtcctggcag ctctccaaca ataacgccga gatacgccgg ttaaagggcc gcattgagga
      661 attgacccgc caaaaggagg cggcctatgt gggatgggag tttgacggcg gcactgtgga
      721 gatcaaccgg gtggccaacc gcctgcaaat cttctttgag gggaagccgg atgctgctgt
      781 acgcgacgag ctgaaaagca acggtttccg ctggtcgccc aaggccgggg catggcagcg
      841 gcagttaaac gacaccacaa tccgcgtcgc tgaccgtatc aagtgcatcc agcccctttc
      901 cggcgaaaag cccagcgcct tacagcttgc ggcccgcagg gagaaagaaa agccctctat
      961 ccgggcgcag ctcaacgcgg ccaagaccga acaggccaag cagccaccgg cgcgggaaaa
     1021 atcaaaggga ttggaggtag agtaaatgca gaagtttgaa aatgtggacg ttctggccgc
     1081 gctggaacag atcatgcggc agaacaccgc gttttatcaa agtgactttg gcattgacaa
     1141 agacattatc aggcgggatg ccgccagcga tcaggccgca gacaagaccc tgctctggat
     1201 gtcccggccc tccgggacgt actgtttccg ggaacgtgat gttttcctga aagacacccg
     1261 acagtacaac acatggaaat tttacgggga gcagacccgt gacaagatac tggcctacgc
     1321 ggtggagctg accggcacac agggcggcac tatccggggc aatctctatg agctggacta
     1381 tccgcagcat ttccggcacg tcattgaaac cgcccagccg gtgagtgtga accggctctt
     1441 ttatgagcat ggcacccgtg acatcccgga gagccagcgt tttgacggaa gccctgaccg
     1501 ggtattggga aactttttgt actatgaggc ccagccccat gacccggctg tgctgcagga
     1561 ggcgctacgg acggagcagc acagccgggg gcgggccgtc cccggcgatt tcaaagtcca
     1621 cgtcaccgct ttgcatgaca gccggattga aaccgaggcc cggcgtattg tggacagcct
     1681 gaaagatctg tccgcgccta acagccccaa taaaacgcat ttcatggtgg agatctcccc
     1741 gcactttgcg gcgattgctt ccaccaagga taatgaccgg ctctttgcca tgctgcccta
     1801 taaatccctg tgttttaccg ggatgaaagg caggcatggc gtgtatgccg tgattaaccg
     1861 ggacgagcgc cgggatgtga gtattcgcag gccccggccc tctatccgca agcagctatc
     1921 cgacagcaaa caggccgcca gcccaaaaaa ggcggcggcc agaaccaaaa agaatgaatt
     1981 ggaggtataa ggaatgatgc actttaccgt ggaggaagaa aacctgatct gtatgtacca
     2041 caatgcagac cggcgcagaa cgataaacag gctcaccgcc gtcatgccgg ttatggatgg
     2101 ggatatgcgg gcgcaggccc agcagaccct ttccaagctg gaacgcatga cggatgccga
     2161 ctatgacggc cagaagttcc acttcacaga cgaataacga acagcagacg ccgggcgcgg
     2221 cggccatgtg gtcgcttgcg tccggcgtct tttctttttg cctattccta cgcatttaga
     2281 acccggtatt gtcggggata ggatgaagta ttcgcttgcc cctgcaaaca tgcgggagaa
     2341 gccttgcagc gggcggtttt caatccctta aatgcgtagg ttgcagctat aattagaccc
     2401 ctgaaacaga tatagccgcg cagcgggtgt atctgtttca ggggtgtgca ggtgatagcc
     2461 gcaaagcggc gtaagagggt gcagccctct tgccgtctat ctctgttcca gcgggaagct
     2521 cggcaatgct tccagcagtt tcagggaaat gcgctgccgt atatcttcgt caatgacttt
     2581 cttctttttg ccgtttgcct gtctgatctc caccgtggaa agctcgttga tgtagtcagc
     2641 ataatgggcc aacacttttt ccgtggccca cctctcaccg gcaacggcgg ctttgatcgt
     2701 ttcatagtcc agcagctttt cttgtccgtc catcctgata cccccgtcag actgattttt
     2761 tctatgatac catattcgga agcatggaac cagagaaaac ggcgcttttc tgctggtaaa
     2821 ttctacgcat ttagaacagc acttcggcgg ggataggata aaacacccac ataccccgct
     2881 gaccatgccg gaaaagcctt gaaatataag ggcttttaca gctctaagtg cgtaggtttc
     2941 cagtatcttt tccgctggcg ctcggcctct ttgcggcggc gcatccgttt ggagcaggcc
     3001 gcgcagtatt tggcccggtt agacttcggc gtgaacgtgg ccccgcactc acagcagcgt
     3061 ttcacttcgt cccggtcttt ggtgatctcg gcatacaggg ccgcatccag cgggaggaca
     3121 gcggccttaa accagcggca cagaagcgaa taggaaatgc tctgtacgca aatacattct
     3181 tcgccgtcgt ccagcaacag gcagttgccg tcacaatagt tgcagcactc atgcgtcagc
     3241 ttccgggcct tgcgatattg agggtaattc attcgtggaa tactcatagc tcttgcgtcc
     3301 attcctttcc cggcgtcata cgcaaaatgc cgtccacatt ctgcttgatg atgccgtatt
     3361 cctgcatctg ctttttcgct tcgccgtact tctcatacag ccgttccttt tcctcggcca
     3421 atcgcttgta ctcggctttc agggcgtaca gatcggggag ctttttcaca ccctgtccct
     3481 ttagggcctt tgccgcagct tcatacaaaa tcaacgtgct ttcatgctca cgccggaatt
     3541 ttgccttatc cgctgcattg cggtattcca tagccacggg ccagagctgc ttgtaggtag
     3601 aaatattctt tatcagcacc gccatatccg caagccggtg ttccatagct ttgagggtag
     3661 cggcggcctc gtcgctggcg gcggtgatct cggccacttt gctttccaaa tccgggtagg
     3721 tgtctatccc atgttcagtc aggaaattca aagtctttgc ggcctgcttc aaattgtgga
     3781 gcttcgccca cctctcatag cctgccgact gctgggcctt aatgttgttt tccagatcaa
     3841 tccgcaaaga aatccccttg cgctctttgg gcgctctggt gataacgcgg cctttgatac
     3901 gctctttgat ggcttcctcg gtgtaggctt cccccaatgt cttgcagcgg gtaaagcgtt
     3961 cctgtccggg agcgcggaaa gaaataaact ttcccggctt gatctcatag ccctgcgcct
     4021 gcatgagccg caggaaatca tcaaaatctt ttgcctgcgg gatagcggcg tcaatggctg
     4081 ctttgagctt cgctttccag ctcgtccctt tccgctgggc gtcccactcg gcatagctct
     4141 tgcccttact ctggccgggc ttcaccacgg acaatccgtt ttccttgcac agcctgtcgc
     4201 tggtgcgccg gatataggca tagctctgtt tgttggaaat gtacttgcgc tgatttacca
     4261 tatccacggc acagaaaata atgtgattgt ggacgtggcc cttatcaata tgggtagtca
     4321 gcacataggg atatttccct tgcagcactt cctcggcaag ctgcttcccg atctcatggg
     4381 cctgttccgg cgtggcttcg ccgggggcaa aggattgtat caggtggtgg cccagattat
     4441 tgcccttttg gtatgcctgc gccagcagca tttcaaattc aatgtccgct gtttcataag
     4501 agcaggcaaa ggaggccacc agcagcttgc cgtcagtttt gtccgggttc tcgatgtagt
     4561 caagggcttt tttcagcgtt gacttgatag gatgtatctt tgtaactgcc atatctgcgc
     4621 cagcgccccc tttatatcct cgatgtcctg tgcgtacacc ggccccatac cgttcagcct
     4681 ctttgcaatc tgattgacgt tgacgccgat cttctgtatc tcggcggtct gcgcccggat
     4741 gtcgctggtg tccatgttga cgatatagcc gtcaatgagc atcttccggg cgtaggctga
     4801 aaagttcttc gtatgcagca gcgccatttt ctgttcaatc atagcccgtt cctccggcgt
     4861 cacagggacg cgcagcacga tttttcgctt tctgtttgcc atgcttcacc gtcctttcca
     4921 ttcaggggtt tggggatttc cccaacaagc aaaacggacg cggcccggca gggacggggc
     4981 aggccgtttt tccgggagtg gctacactcc ctatgcttgc tacgttactg tttctccact
     5041 aacaagacgt ttccaaagcc ccaaactacc cgcaaaatag ccctctggaa agaaaaaagt
     5101 gcaaaaaaat agaagctacg ctgtgcagct cccgctaaaa gtaaaacaga ggatgccagt
     5161 tacacatcct ccgtttcatt cttcttcggc ttttcaatta gtatggcgct gtcaatcgcg
     5221 ccctcgatga tcggcaggta ttcttcgggg cagagtttca gcttgtggct gaccctttgc
     5281 cgcagttcgc tttcctcccg catgatctcc gggttaaagt agcgttccac aggaagcccg
     5341 caggctttaa taagctgtat gacaaccggc agactgggaa tggcccgtcg ttttcgatgt
     5401 tggcaagata gcgccagtca atatttattt tctctgccat tgctttgcgc gaaacgcgct
     5461 ttgcttgccg ggccgctttc acatccgcgc cgaaagtttc aaagccggga caatcttcaa
     5521 tattcgccat atcgcacctc ccacttacat tgtatatttc atactctgcc cgtggaatgt
     5581 tggtatatgc acctttctta aagtttatat ttcacatttt gtttctttta ttcttcgagc
     5641 aaaagaatta taatgtaccc tgtggaggtg tactatggac tacatgacgt tgaaagaggc
     5701 aggcgagaaa tggggtgtgc ccccacgatg gataaactac tattgtgctg cgggacgcat
     5761 ccccggcgct gtaaagatgg cgacgatctg gctcattccc aaaactgcgg aaaagccgat
     5821 tgacaggcgt acaaagaaag gaaaggcggt tactgaatag tataatgaaa ccggcgcatt
     5881 ttggatatgg ttttgaagag agagaaagca tggtattttg tcacggaaaa actataagat
     5941 gatagatggc aggctgctcc agaccgataa aaagtattct catctgaaac agaaagaaaa
     6001 gatttacaca tggatgtatg aagaagcgaa actttatcat gacagaaatg gaaaatgccc
     6061 gaaacaaaga cgaggaagct gctgctgtat cagccgtata tggtagaatt gaaaaggccg
     6121 gcatctggat tctttatggt gaagtctcca gacattggat aactgctggt ctaatgcgcc
     6181 tttgagtatg gggtgattga ttttaatacc ggggaggtac ggctttttga ggaaaatatt
     6241 ctgggattta catttggcga ttattacatt tcctgtaact gcaaggacac ggatgatgaa
     6301 gatatgagga atatagactt gctgattttc tactgtcttg aacggtatta ggactgaatc
     6361 tgctgcgcgg agtgcggcaa gagcgaccgg agggagtaca cacaggccct gcctgtgtga
     6421 aaagccatac cgcaaggtgc attcgcaaaa aagaggccgc gcattttgct gaggcttggg
     6481 gtgtccccaa caagcaaaac gagaacgccg caggcgggca agtggtggat cagaatatcc
     6541 gtaaaggata ttctgatcca ttgcttgcca gtattatcgg gatgttttga caaccgctgt
     6601 aatcgaaaca tcccaaacat taagaaggtg ttaattatta ttatcttgat ttaattcgtt
     6661 taaaccaata atatagatac atacaccatg aaaagaagcg agtatcccaa tgacggatga
     6721 ttaaaatccg aaaagaaatc gctttgaaac tacagggaaa ctacagactg ttatgttatc
     6781 ttcttaaaat ggagggattt ttatgtcgat acgaattcta cttgtcgagg atgatgatca
     6841 tatctgtaat acagtaaggg cgtttctggc tgaggcagga tatcaggtgg atgcctgcac
     6901 agatggaaat gaggcataca ccaagtttta cgaaaacact tatcaactgg ttattcttga
     6961 tattatgctg cccggtatga acgggcatga acttttgcgt gaatttcgtg cgaaaaatga
     7021 tactcccatt ctgatgatga cagccctgtc ggatgacgaa aaccaaatcc gggcgtttga
     7081 tgcagaggca gacgactatg taacaaagcc attcaagatg cagattttac taaagcgggt
     7141 ggaagccctg ctgcggcgca gcggtgcgct ggcaaaggaa atccgtgtcg gcaggctgac
     7201 acttctgccg gaggatttca cggtactttg tgacggtaca gagctgcccc tgacacgaaa
     7261 agaatttgaa atccttttgc tgctggtgca gaacaaaggc agaaccttaa cgcatgaaat
     7321 cattttgtca cgtatatggg gatatgactt tgagggtgat ggcagcacag tccacactca
     7381 tatcaaaaat ttgcgggcca agctgccgga aaatatcatc aaaaccattc gcggtgtagg
     7441 ataccgattg gaggaatcat aataatggaa agaaaaggga ttttcattaa ggttttttcc
     7501 tatacgatca ttgtcctgct actgcttgtc ggtgtaacag caacactgtt tgcacagcaa
     7561 tttgtgtctt atttcagagt gatggaatta cagcaaacag taaaatccta tcagccattg
     7621 gtggaactga ttcagaatag cgataggctt gatattcaag aggtggcagg gctgtttcac
     7681 tacaataacc aatcctttga gttttatatt gaagataaag agggaagcgt actctatgcc
     7741 acaccaaatg ccaatacatc aaatagtttt agacccaact ttctttatgt ggtacataga
     7801 gatgataata tttcgattgt tgctcaaagc aaggcaggtg tgggattgct ttatcaaggg
     7861 ctgacaattc ggggaattgt tatgattgcg ataatggttg tattcagcct tttatgcgcg
     7921 tatatctttg cgcggcaaat gacaacgccg atcaaagcct tagcggacag tgcgaataaa
     7981 atggcaaacc tgaaagatgt accgccgccg ctggagcgaa aggatgagct tggcgcactg
     8041 gctcatgata tgcattccat gtatgtcagg ctgaaagaaa ccatcgcaag gctggaggat
     8101 gaaatcgcaa gggaacatga gttggaggaa acacagcgat atttctttgc ggcagcttct
     8161 catgagctaa aaacgcccat cgcggctaca agcgttctgt tggagggaat gcttgaaaat
     8221 atcggtgact acaaagatca ttctaagtat ctgcgcgaat gcatcaaaat gatggatagg
     8281 cagggcaaaa tcatttccga aatactggag cttgtcagcc tgaatgatgg gagaatcgta
     8341 cccatagctg aaccgttgga catagggcgc acggttgccg agttgctgcc cgattttcaa
     8401 accttggcag aggcaaacaa ccagcggttc gtcacagata ttccagccgg gcaaattgtc
     8461 ctgtccgatc cgaggctgct ccaaaaggca ctatccaatg tcatattgaa tgcagttcag
     8521 aacacgccgc agggaggcga ggtacggata tggagtgagc ctggtgctga aaaatgccgc
     8581 ctttttgttt tgaacatggg cgttcacatt gatgatactg cgcttccaag gctgttcacc
     8641 ccattctatc gcattgatca ggcgcgaagc agaaaaagtg ggcgaagcag aaaaagtggg
     8701 cgaagcggtt taggacttgc catcgtacaa aaaacgctgg atgccatgag cctccagtat
     8761 gcgctggaaa acacctcgga tggcgttttg ttctggctgg atttaccgct cacatcaaca
     8821 ttgtaaatat ttaaaactta aatgattttg ggctctttgt caaataggac tgatagagtg
     8881 agataagaac atctaaggta gaattccttt tctactttag atgttttttg attcataggt
     8941 aatcagatta tttagtaaga aaattctggt cttcattttc cgaaagcttc gaaagccata
     9001 agcgactcgt ttcaatgctt tgatgtgggt atgcagattt tctattttgg catttgaata
     9061 aggcaattcg agggctaaac gaatgccgga ttcatagttt ataaaagctt tttttataga
     9121 atgcagttct tttggaacgg aattgggcat cgttcttaca agtttaaaga aagtgtccgc
     9181 ctctttgtcg tgaaacgctt gaagaatttc ttgatagtaa aaataggcga ttcgtaattc
     9241 gtcagagatc gaaagcagac gatccaccac ttcactttcg gtgagatacg cccaaccaaa
     9301 attgcgccat ttggtccatt tgtcataaac aagcttgtcc tgtcgtttga tcagaagccg
     9361 agacaacgcc tttaattgtc gataatattt ggcttgtgtt ggatcgtttc gatcaaagct
     9421 tttcatgatt cgtacgcgaa tatcctcaaa tttacgaatg agatgtttga caatgtgaaa
     9481 acgatccaca acgagttttg cctttggaaa acattccttt atcagagagg cataagaaac
     9541 attcatgtcc gtcacgatga cctttacctt caatcgtgcg cgtcgttcaa attgcataaa
     9601 ataagaaacg agagcctttt tcttccgtga aggaagaata tccagtaatt ttccagtatc
     9661 gccatccata caagaaaagg agtagggacc gacttgattt ttcagtgaac gaaattcatc
     9721 cattaataaa caacgcggta accaatttct ttttactttt gtcatgggga tcagagaagc
     9781 gagtgtccga gaaacggtcg accaagaaac gccgcaatgc ttagcaattg tcgtcattgc
     9841 ttgtttttca gttagctctt ctaaaatttt atagcgcact tgtttagcga taaagcagtt
     9901 tctatcgaca atgtaggtat tcgccgtaaa ataggtgtgg cagccacgac agtggtagcg
     9961 ttgtttcgct aaagcaagat aagtcagttg gttcgcacat ttattaagtt gaatgagcga
    10021 ttttttcttt ccgttcttaa cgatcatgcc ttcatgattc atctgtttac acaaaggaca
    10081 gcgacgaatt tctttactta acgtcgcaga aacaacaaga ctcttgatat tcttaatgct
    10141 cttcgattcc acatttgtga tgaaaatgtt tttatctttg attccaagta gtagtttgat
    10201 atgatgaaga tcaggcatac agaacgctcc tttgattgtg ttgtggtgat tcaattctaa
    10261 cggagcttct gtatgttttg ttttttcatg ctcaaaaggg accgatagaa tgattttcta
    10321 tcagtcctag aaattataga gccgatttt
//
LOCUS       DANJMI010000185         9769 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.guided.087, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000185 DANJMI010000000
VERSION     DANJMI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.guided.087"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            complement(<1..801)
                     /locus_tag="PWU81_003137"
     CDS             complement(<1..801)
                     /locus_tag="PWU81_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL1111738.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLI"
     gene            929..1498
                     /locus_tag="PWU81_003138"
     CDS             929..1498
                     /locus_tag="PWU81_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL1111739.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1565..2302
                     /locus_tag="PWU81_003139"
     CDS             1565..2302
                     /locus_tag="PWU81_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL1111740.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            2533..3801
                     /locus_tag="PWU81_003140"
     CDS             2533..3801
                     /locus_tag="PWU81_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL1111741.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4288..4752
                     /locus_tag="PWU81_003141"
     CDS             4288..4752
                     /locus_tag="PWU81_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL1111742.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            4736..5365
                     /locus_tag="PWU81_003142"
     CDS             4736..5365
                     /locus_tag="PWU81_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL1111743.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            5719..7197
                     /gene="msr(C)"
                     /locus_tag="PWU81_003143"
     CDS             5719..7197
                     /gene="msr(C)"
                     /locus_tag="PWU81_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL1111744.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7244..7690
                     /locus_tag="PWU81_003144"
     CDS             7244..7690
                     /locus_tag="PWU81_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL1111745.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(7889..8404)
                     /locus_tag="PWU81_003145"
     CDS             complement(7889..8404)
                     /locus_tag="PWU81_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL1111746.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            8521..9723
                     /locus_tag="PWU81_003146"
     CDS             8521..9723
                     /locus_tag="PWU81_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1111747.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         3108 a         1721 c         2037 g         2903 t
ORIGIN      
        1 aatgagattc agtgaagctt gtaatggcgg atcgagtggg atgaatactt tttccgtccc
       61 ataagtattg atgatcccat ctagagctaa tgcaccaaaa gtcccgttat cgaccatcaa
      121 agaagcattg tacaataagt tatatgttcc gacgatcgat aactgatcta tgctgaaacc
      181 tagccattca gctagttgac tgtccactga cgtttgccgg gaaatgatca aaggaacagt
      241 tttcagttcc tgcggatgga tgactgtttg ctttgctaaa ggatgattcc gatgtatcaa
      301 aagtccccaa cgatcttggg ctggcagtcg aaatgactgg tatttttgtt tgtttgtcgg
      361 ttcgattacg agtccaaaat caagtaatcc gctatccagt ttgtctatga tatcatctgc
      421 attcccgcta tataaatgaa actggatatc aggatgcgcc ttgatcaacg aatgaaggct
      481 atcagaaatg aaagagattg ctttagtttc gcctccgcca atgtagattt ctccagagat
      541 gattgcgtca tttgttaaat tgttcgccgt tttttctacc aacgacaaga tttcttttcc
      601 ttttgaataa agatatcgac cagaatccgt caactgtatc tctcgggagc ctcgagtgaa
      661 aagtgtcaca ccaagttctt cttctaaatc tttcagttgt tttgagagag taggttgcgt
      721 gatatgcaga gattttgctg ctcggctgat atttttttct ctagctactg ctaaaaaata
      781 cgtcagtata cgaatatcca ttttttccct cctgttttta aaaaagcata ccactaagta
      841 tagttaaaag gaatagttaa gtatgtttta taagtattag acatcataag ccgtccgtta
      901 taaagtaagg ataaaaggag ggagcaaaat gacaagcctc atcgaaaaac aaatcgtcaa
      961 taaagagatt cgagtaacgg atccgctctt tgaagtgatc catcagatcc aagcggaaaa
     1021 cgaagagcct ttagcagcgc tgaatactgg gtaccatgaa ccagcagata tccgcaaacg
     1081 gctagaaaat atcattagtg acaaagtaga tgatacggtg actgttctgc tgccttttta
     1141 tacagatttc ggcaaacata ttagtatagg gaaaaatgtc tttatcaatc gtcaagtgat
     1201 gttcgttgac ttaggtggca tctgtttaga agattcagta ttgattggtc ctcgtgtcaa
     1261 cctcatcacg gtgaaccatc taaccgatcc aaaagaacga agaggcttgt cagtaaagcc
     1321 aatccacatc aagaaaaatg cttggatcgg agcaggagca actattctgc caggcgtaac
     1381 gattggcgag aatgccattg ttgcagcgaa tgcaactgtt acgaaagatg tcccagacaa
     1441 taccatcgtt gcaggaatcc ctgccaaaat cgtcaaacct gtcgaacgga ttatttgaac
     1501 agtaagatga aataagagat agtgagtata agcaagagat ttttcaaaac aggaggagaa
     1561 tagaatgaat gatttatcag aaaaagtgat tgtcatcatg ggcgcttcaa gcggaatcgg
     1621 tgaagcaaca acgaagctat tagcagaaaa aggggctaaa ctagttatag ctgcacgccg
     1681 agaagatcgc ctaaaagcaa taaaagaatc tttaccagaa gctgagctat atattcaaac
     1741 agcagatgta agagattttg cacaagtcca agcagttatc gatttagcga tggaaaagtt
     1801 cggtcgaatc gatgtattgt acaataatgc aggaatcatg ccaaccgctc cattagttga
     1861 aggtcaccgt gatgaatggc aaaatatgct tgatatcaac atcatgggtg tgttaaatgg
     1921 aatttccgcc gtacttccaa tcatggaaaa acaaaaatct gggcatatca tttcaactga
     1981 ttctgtggcg ggacatgttg tttatcctga ttccgccgtt tattgcggaa caaaatttgc
     2041 tgtccgtgca atcatggaag gattacgtca agagcaacgg caaaataaca tcaaatctac
     2101 gatcatctct ccaggagctg ttcaaactga attatatcaa accatcagcg acaaaaaggc
     2161 tgctcttgaa cttcatgaag cgcaaaaaga atggggatta acatctgaag atatagcttc
     2221 tgctgtagca tttgcgatag aaacgccaga ccggatgtct gtcagcaaca tgattatccg
     2281 tccgacgaca caggaagtat aatccataac ggataatatc cagagagaat tacttgttaa
     2341 atagttattc aaacgaccat caatttaagg cgtgaatgga gaagaatatt cttcatttac
     2401 gtctttttat gcagaaaaag caaatttatt aaaaagaaac aaatgttatt taaatatgga
     2461 cattgacttg cttttacttt tttagttaca ctgttattta ggaaaagttt cataattcaa
     2521 aggagaaaaa agatgaaagc aaagaattta ttatttagcg ttattctttt aggaaatcta
     2581 gcagttgcca acacaacagt gctagctgaa gaatcgacag aaactgtcgc aactcagaca
     2641 tcgagttctt cagaaacagg aattgaaaac gatacagaaa gtactttgca gtcagctact
     2701 gtctcagaaa atacaatttc aaccgaagaa ccaacaactg aaacaacaga acaaacacaa
     2761 accgaagaca gtacatcagc agaagctcag aacaatccag agaatgcaga agaaatcacg
     2821 aaccaattga cagatgggca aacattgaca catacccatc aagcagattt gtctgaattc
     2881 gaaaactata ctggctatgc tttttatgct cgctcatctg ctaattctca acaagcgttc
     2941 attgatagta ttgcctcaac tgcacaaagt ttggcaagtg ccaatgactt atatgcatca
     3001 gtcatgattg ctcaagccat cgttgaaagt ggctggggga acagtacttt ggcttctgcc
     3061 cctaattaca atctatttgg gattaaagga agctataatg gccaatctgt tacgatgcct
     3121 acttcggaat atgtgaatgg gcaatggatt acggtgaatg ccgcttttag aaaatatcct
     3181 tcgtacaaag aatcattgca agataacgta acggtattga agacgacttc ttttcagcca
     3241 ggcgtttact actattccgg tgcatggaaa agcaatacga actcttacaa agatgctacg
     3301 gcttggctaa caggtcgtta tgccactgcg cctaattatg gttctacatt gaacaatgta
     3361 atcgaaacct ataatctgac acagtatgat acagcaccaa cttcaacttc tccgatgtat
     3421 cgtctgtata accgtcatac aggagaacat ttatatacat tgaatgccgg agaaaaaaat
     3481 tatctgccaa ctgtaggctg ggaatatgaa gggattgctt ggcaagcacc aaacagcgga
     3541 caacctgtct accgtttata caatccttac agtggagacc atcattacac aatggcacaa
     3601 tctgagatca actctttaac aaaaatcggt tggcgttatg aaggtctaag tttttattct
     3661 ggtggttcca agccaattta tcgactattt aatccaaatg aaaaaactgg tacccatcac
     3721 tataccttga gtgctaagga aagagattgg ctaacaccga tgggctggag atatgaagga
     3781 ataggatttt atggttatta aaaagaggta gtgaaaaagc tgccctgcat caagtagtaa
     3841 gaacagccaa acaaaaacag attttcatgt tttttgtttg gttgaactta ttaccgcaga
     3901 tgcaagtttt tgaacaccgt ttactcaaaa aaggttgtga caacatcttg tcataacttt
     3961 tttattactg tttaagcata ctgatccaat ttctaatatt cgacgggatt cttgcaaaag
     4021 gcgatttcgg aaatttttgg cggtgtaaaa ttatctactg cagaagagct atcttctata
     4081 gtttgataag aatcaataat cttggtttta gcgttgtgct ttctttatca ttttttccgt
     4141 tcattctaca aaaaatttct tttctctata cttttctatt gagagcgttt tttttattac
     4201 acagctgatt ctttgacttt tttctttttt tattctatta ttgacaagtc aatcgttgac
     4261 gagtcaatta ttagaaagga ggaaattatg tctttagaag agattagtca ccttttatat
     4321 caaatcaaat tagccgatca aaaaatcgtc cagatctttg aagaaaatat cggtataagt
     4381 cttactagat acgagatcat gatgagactg aaagaagaaa atccattgct acaaagcagt
     4441 ttgcaagaaa agttgcagat tgatcaagga gcagttactc gtcatctgaa ggtattggaa
     4501 gcaaatggct atgtttcgcg tcagagaaat cctcagaaca accgggaagt gtatgtagcg
     4561 ctaacagaaa aagcccagaa gaaaatcaag cattgtgaaa tggaccataa agatacaggt
     4621 tccgtgctat ccccggcttt cagcgatgta gaaatgaaac aatttctaac acttttagat
     4681 aaattgaata cccaactttg caatataaca acagaaacga aagaggttaa taataatgga
     4741 acaaaaaaat gatttttcaa caattatgac tggtagaaga tccgttcgcg tatatgatga
     4801 aacatacaaa atcccacacg aagaaatgct ggaaatgatc aaagaagcga cgactgcacc
     4861 ttcttctgtc aatatgaaac catggcgctt tgtggtcgct gaaagcgagg aagcaaaaga
     4921 aatcttgcgt cctttgatcc gtttcaatac gagacaaaat gatacttctt ctgcaatggt
     4981 catgattttt ggcgatatgc aatgttacga atatggcgaa gaaatctaca accaagctta
     5041 cgaatcaggg aagatgccaa aagaagtcag agatcaacaa ttagctgcga tcattcctta
     5101 ttacaagagt ttttcaagag aagaaatgaa cgatgtagtc aaagtcgatt ctagcttagc
     5161 ggctatgcaa ttcatgttag ttgcgcgtga tcatggctat gaaacaaatc caattggtgg
     5221 attcgaagca gatcagttgg cagaggcatt tggtttggat aaagaccgtt atgttccagt
     5281 tattatttta tcaataggaa aagcggtaga agaaggttac gagtctgttc gtttagcacc
     5341 agaaaagatc actatgttcg aataaaaggt tttcagttac tattcagtag ctattgcttt
     5401 ttttgaatcg tgtgtggtaa cctaataaaa aaggaggtcg gaaaaatgac tgcatcgatg
     5461 aaattacgtt tcgaactttt gaataacaac taaataaatt caaaagacat gtataaacaa
     5521 caatttcatg gatggagtat gtccattttt tcgaacgaca gtagaatagg tgaaagctat
     5581 gacagatttg gtgatttagt catagttatt tcgttcagag taggaaaaga tgtgatgcag
     5641 tttttttgca aacagatttt tctgctcttt tttagtatcg ataattgttg ttttacatga
     5701 aataggagtg taaaacatat ggaaaattta gcagtaaata taacaaatct acaagtcagc
     5761 tttggcaacc agctagaact atctattgat tctcttcgtg tctatcagca agatcgtata
     5821 gggatcatag gagaaaatgg tgtcggtaaa tcgactttgc tcaaactaat agccggtgaa
     5881 ctttttcccg atcatgggaa aatccaaaca gagatcacct tcaactacct gcctcaatta
     5941 acctatctcg ctgaagcaaa ggacctaaat ttggaattag ccagtcattt ccagttaaga
     6001 ctggaagaaa cttcagagcg gaaatggagc ggaggggaag aacgaaagat cgagttgata
     6061 cgtcttcttt cttcttatga acaagggatg cttctagacg agccaacaac ccatctagat
     6121 agaaaaagta ttgatcgact gattgaagag cttcgttatt attatggtac gctggttttt
     6181 gttagtcatg atcgctattt tctagatgag ctagcatcga aaatctggga agtaaaagac
     6241 ggagaaatcc gagagttttc ggggaattat agtgcctatc tcactcaaaa ggaattggag
     6301 aaaaagactc agctacgaga agcagagtcg atcatgaaag agaaaaaacg attggaaaaa
     6361 tcgatccaag aaaagaaaaa acaagcggaa aagttggaaa aagtgtccag taaaaagaaa
     6421 aagcaacaaa tcagactgga tcggttgtct tcctctaaac aaaaagacag tgtacaaaaa
     6481 gccatccaaa agaatgcgaa aacattagag agaagactcc aaaaaatagg agaaacaacc
     6541 aagccgcagc agatgaaaca aatccgtttt ccagtaccaa aatctcttga actccacagc
     6601 cgctatccca tcatgggaca aaatgtccaa ttggaaagaa gtgggagaac attactggta
     6661 aatggtgatt ttcagttttc tttaggtaaa aaaatcgcga ttgtcggtga aaatggttca
     6721 ggtaagacaa ccttattaga acatatccgc aaacaaggag aaggaatcct tctctctccg
     6781 aaagtaagct ttcaagtata tcagcaaaag ggttatcaaa tgacatctga agaatccatc
     6841 attcgttttg tcatgagaca aacagagttt tcagaatcgc ttgtccgtag tttgctgaat
     6901 cacttagggt ttgctcagga aactttgacg aaaccgttat gtacattaag tgggggagaa
     6961 gcgacccgtc tgacgattgc tttgcttttt actaagccaa gtaatgtgtt gctgttagat
     7021 gaaccgacta attttattga tatggcaacg atcgaagctt tagagaagct aatgcaaata
     7081 tatccgggaa cgattttgtt tacttctcat gattcttact ttgtcgagcg tacggctgat
     7141 gaagtttatg aaataaaagg gcagaaaata aaaaaagtac ttacgagaaa tttttaaatg
     7201 tgctacgatg attttcgtga caaatttaaa ggagtggtac agattgccta caaataaaaa
     7261 agaaggaata atttttacaa caatgatgtg ttttttgatg gtcttgggga tgagcgcgta
     7321 taatttatgg ctgcatcaag atcttcattt atcaagtttg ttgattggtc tagtgcctgg
     7381 attcgtggtg gctttcattt tggatgtctt catcatcggt gtgctggcga aaaaaattgc
     7441 ctttaaacta ccagtaaatc cagaaagcaa ggtccaaatg atcttaacta tttcatgctt
     7501 gatggtttta ggaatggtga catgtatgtc tttattcggt gtgatcatgg aacaaggaat
     7561 acctgatcaa ttattctctg cttatatgaa tgcttggaaa atgaatgtga ttgccgcttt
     7621 accgttgcag ttgatcatcg ttggcccgct ttcaagaagt gtgctaaaag caattcaaag
     7681 taatgaatga tggaacactg ttatttgaaa aaaggccttg gcatgctttt tgtcatagtc
     7741 tcttgaatgc agttctagaa aaatagtgat ccgaacgata ggttttggcc tgtcgttcgg
     7801 atcgcttttt cttttccaac tgtattgctc ttttattgaa gataatttgc tcttcaatag
     7861 atatttctag aaaggttctg tttatccctt actcaaaaaa ctgctgcatt tgttctctta
     7921 cttgctgaca ccattggagt tcctgctgac aaagcataat tctgcgctgg atcacaacat
     7981 aacgtccaaa ttcttgtttc ttgtctagcg agaggtcatt caattttttt tgattttttt
     8041 gcatgatttt gttcagctgt tgttctctag tgaatagcag tccagtagct gttgttttat
     8101 cgagcgtcga aaaagcatag atttttgcta gaaattcatc tttttgcgta ggagcgggag
     8161 tttcctcttt gatccattcc tctacaagag cacgtccttc atcagtcaag tggtagatct
     8221 ttttttgaga gtgctcgtct ttgtccaaaa cggtcacata accctgctgg tctaacttgg
     8281 caagcgttgt atagatctga ctgtgatggg cttcccaaaa aattgtgatc agctgcttca
     8341 attcataccc agaacgtggt ttggataaaa gcacgcataa taatatatag gctaaagtat
     8401 tcataaaaat cctacctctc gtcttatttt ctttattata tcacagtaaa gtttccacgt
     8461 tttacatgtg catattgaca tgtaaaataa atcatgtatg atgctaatag agagaaaggg
     8521 atggaaatat tggagaatca aatagatata caagaacaaa caaatgaaga aacaaagggg
     8581 aaaagttcag cgctagttgt agcagctacc tgtatgatcg cttttatggg agtgggtcta
     8641 gtagacccaa tcttaaaaac gattgctgca cagttaaacg ctacgccagc tgaaacgacc
     8701 ctgttattta ctagttacat gttagtcact ggtgtggtga tgctatttac aggtttctta
     8761 tctagtcgga tcggtttgaa aaaaacattg atgacaggat tgtttatcat cgttgtattc
     8821 gctggtcttg gcggattatc tgggaatatc ggaatgctga ttgggttacg tgccggctgg
     8881 ggtgtcggga atgctttatt tgtctctact gctttagcaa cgatcgtcag tattttagtt
     8941 ggtaacacag aaaaagcaat catgatgtat gaagcagcac ttggctgcgg aatggctgtt
     9001 ggccctctag tcggtggggt cttgggcagt ggttcatggc gttatccatt ttttggggta
     9061 gcatttttga tgttttgcgg ttttatagcc ctagcggtac ttttacctga aactgagaaa
     9121 ccaaagagaa aagtaggcct ttgggaaggt gtcaaagcgt taagcaatca taaacttcgt
     9181 tcagttggac taatcgcatt gttatataat ttcggattct ttactttact tgcgtatgcg
     9241 ccatttttat tgattggcta ttctgaatta gaagtcggtt ttgtattttt tggctggggt
     9301 gtgcttctgg ctattgcttc gattttcgtt gcaccggctc ttgagcggaa gtttacgact
     9361 tatcggacaa ttttagcagc atttgtctta ttcattattt gtttgctgtt attaggagct
     9421 ggaacttcta tgccagtcct tgtaccgatc agcattgtgt tagcaggatt tttccaagga
     9481 attatcaata cactgttgac gacgattgct atggaaatac caggtctgca acgaaacgtt
     9541 gcttcgtcca gctatagttt tgtccgcttt ttcggtggag ctttagcacc atttattgca
     9601 ggtgtgattg gtgaacatat tggtgaaagc tatgcgtttt attttgcagg tgccattgtt
     9661 cttatcagtg tatgttgtat catttatcac cgtaattatt ttgctacaaa tgaagaagaa
     9721 taagaaatca gaggttgtga aaaagctgtc ctgcatcaag tcataagaa
//
LOCUS       DANJMI010000186         6173 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.guided.105, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000186 DANJMI010000000
VERSION     DANJMI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.guided.105"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            <1..592
                     /locus_tag="PWU81_003147"
     CDS             <1..592
                     /locus_tag="PWU81_003147"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1111748.1"
                     /translation="DVSTKMFCEKGYDNTSLQDIINETKLSKGAIYHHFDSKEDILKA
                     IFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSVLLTVSPQLLNNPRFL
                     AMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMVLSNVWLNPLVEMTDK
                     EGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            699..1937
                     /locus_tag="PWU81_003148"
     CDS             699..1937
                     /locus_tag="PWU81_003148"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL1111749.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            2164..2583
                     /locus_tag="PWU81_003149"
     CDS             2164..2583
                     /locus_tag="PWU81_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL1111750.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            2588..2827
                     /locus_tag="PWU81_003150"
     CDS             2588..2827
                     /locus_tag="PWU81_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL1111751.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(3052..3975)
                     /locus_tag="PWU81_003151"
     CDS             complement(3052..3975)
                     /locus_tag="PWU81_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDL1111752.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4007..4771)
                     /gene="map"
                     /locus_tag="PWU81_003152"
     CDS             complement(4007..4771)
                     /gene="map"
                     /locus_tag="PWU81_003152"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL1111753.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4929..5369
                     /locus_tag="PWU81_003153"
     CDS             4929..5369
                     /locus_tag="PWU81_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDL1111754.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            complement(5547..6116)
                     /locus_tag="PWU81_003154"
     CDS             complement(5547..6116)
                     /locus_tag="PWU81_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL1111755.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
BASE COUNT         1970 a         1051 c         1094 g         2058 t
ORIGIN      
        1 agatgtttct acaaaaatgt tttgtgaaaa aggatatgat aacacatcat tgcaagatat
       61 tatcaatgaa acaaaacttt ctaaaggtgc tatttatcat catttcgatt caaaggaaga
      121 tattttaaaa gcaatttttc aaagaattgg aaatgaaaat gcagtaatat ttgctaaaat
      181 tagagataac gatagtttaa acggtttaga aaagttaaga gaaatattta agacagcggt
      241 attcaattct aaccaaagcg ttttattgac agtttctcct caactattaa acaatcctcg
      301 ttttcttgct atgcaaatag aacaaatgta tgaaattgta gtacctgaat ttgttgaacc
      361 cgttttagaa caaggtatca aggataagtc tttggagatt gaaaattcac atgaaattgc
      421 agaagttatc atggtattaa gtaatgtatg gctcaaccca ttagttgaaa tgacggataa
      481 agagggtatg gaaaaacgtt gcaatacatt caatgccctt ttaaatggaa taggcataga
      541 cacattatta gacgaagaaa caatttctgc atttatacag ttttgtaaat aactgtattt
      601 gttatcccta tacgatagac tgctctataa aagagcagtt tatttaaacc attatataaa
      661 ccgacggtct gtttgattaa ataaaaagga gagattttat gcaagcagaa aaaaaacttt
      721 ttaatcgtga ttttatactt gttgttatag gacagataat ttctttattt ggaaacgcta
      781 tcctacgatt tgcattaccg tactatttgt taaaagaaac tggttcatca gctttgtttg
      841 gtattgctac agcctgctct tttttaccta tggttatttt gtcgctggta ggtggtatta
      901 ttgctgacag gacaaataaa agaaacatta tggtcatact agattttgta acagcaatca
      961 taattttagt attttatttg tcattgggaa aaattcctat catacctttg tttattgttg
     1021 ctttgatgtt actttatggt atttctggtg catatcaacc agcggtacaa gcaagtattc
     1081 ctctattggt atcatcagat aaacttaccg tcggtaatgc tataataaat caagtaaata
     1141 ctttagcaaa ctttatcgga ccagctataa gcggagtagt ttttcaattt tatggtatca
     1201 atcccatttt aattgttagt attatatgtt ttactttttc agccattatg gaaattttta
     1261 tccatattcc acaccaaaaa agacctcgtg aagtaggtgt aattgcagtt gctaaaaacg
     1321 atttaaaaga tagttatacc tttttgaaaa aggagaaacc catttttata tccgtaatct
     1381 ttttggtttg cattttcaat ttggttttaa gtgctgtttt aattgttggt ctgcctatct
     1441 taatttctga agttttaaaa atgccagata cactatatgg tttttcacaa tcagcattat
     1501 cattaggtgg tttatgtggt ggtattttaa cagctcttgt tgcttcaaag ttaaaactaa
     1561 aaaaccttta taccttgtta cttatttgtt ctgtttctgt tttacttatg ggaatttcgc
     1621 tatttttaga attgcctgca atagtttcct actgggtagt tacagtgatg tgctttgtgg
     1681 ctatgggtgt atctactatg tttgttgttc aaatttacac tcttgttcag acacaaacac
     1741 caccgcaatt agtaggaaag ataatggcta cgctggtagc agttgctatg tgcggtcaac
     1801 ctattggaca aatggtttac ggtattttat tcgatgtatt tatacaagaa acatgggttg
     1861 ttatgtttgt agcttctatt gcttcaatca tgatttcaat atattcaaaa agaatttttc
     1921 aaaagttaga gatataatac aaggggttaa atagtatgtt aggctaaaaa caatatataa
     1981 aagaaaattt gaaatctgct aaaaggatag tgttataaaa gaaagagtaa tattgcagta
     2041 tttaaggctc gcacaaatat atgttttgtg tgggcttttt tcttaccatt tttctgtatt
     2101 tctaaagggt ataaacggta ttccgtttat atatttcagc attagcagaa agggggtgag
     2161 attatgagga catcttcttt cgagcatatc gtcagattac agttcaattc tttaatgctg
     2221 attgtcatta agaacaaagt gaaaagtcga aacagacagc ttgcaagaca ttctaaacgt
     2281 gaagtattgt tttgtgaaat agccgaaacg aaacaagttg aacgtggaac gattgatacc
     2341 tgtttttatg attgtgtatc atttaaagtc ttgcacttta caatttatgt atctgatgaa
     2401 acacttggta cagttttaca tagactgtca gaaaaacagc gtagtgcgat tttattacgt
     2461 tattttcaag gaatgaacga cagagaaatt tcagaactgt accatgtatc acgttctgct
     2521 atttgcagta gaagaactag aggactaagg aagttaaaaa tgcttttgag cgaaaggaag
     2581 taaagaaatg agtaaagatt atggctatcc ctcttttgaa ttgatttgca aagcgtcaag
     2641 cggagatgaa atggcagtta aagagatttt gaagttttat gacggttata tttctaaatt
     2701 atgcttgcgt cctttttacc actctgaaag tggtaaaatc attatgcagg ttgatgaaga
     2761 actaaaagga gaaattcata cagacatgat gaaagcaata ttgaaatttg aaatcggagt
     2821 gaagtaatca actgtataaa aaaatcggga gatacacagt ttcgtgctat ctcccttagt
     2881 agttttacgg tcatatcaag gctcattcaa tcgtggtaaa ttcgtggtaa gatgagtgtt
     2941 tttctatact tcaaaatgct tgaatgtata gtgtttatgc ggataattga aaattagata
     3001 taaaatttca aataaattac tgatataaat ttacaataag aactcttgtt tctacttttt
     3061 tttatgagaa aatttagttt tcaatttatt gaaccatttt cggcttggat ctcgacgttt
     3121 tttgatttga tgacccgtca cgtattcttg aacaactgca ttgatgattc cgcccaaaat
     3181 gatgatcgta gcagcaaaat taagccaaag catgagtaca ataaaacttc ctatgatctg
     3241 ataaccgctg atcctcgttg caaaatacct agcatagata ctaaacactt gtgataacag
     3301 catccagccg acagtagcga agatcgtgcc cggaatgatc gtcttcaact tcaactgagc
     3361 attagggaca attaaataaa tcaagaacat gatgacaaat aaagctacca atgtgattgg
     3421 ccacttcaaa gtttggaact gatcgataaa gttcacgggt atttgtacga ttggctgaat
     3481 agcatccaaa atcagctgtc ccaacccaag aacgatagtt accccactaa tagcaatcat
     3541 gaacaagaca atgacaatta gtgagaagaa acggacgatc aaaaaatttt ttctattttc
     3601 tacgccatac gctttattca tcgcaatttg caaagcattg atactttgac ttgcagacca
     3661 taaagccgct aatgcagaaa cggataacaa gcctcctgaa ctttgtgtca aaaggtcttt
     3721 gattgcagga ccgataaact ggtaaacggt ctcaggaatc acttctcgta aataggtcag
     3781 tacagtctct tgatcgatgt ggagaaacgg taaaatatta ccaaaagcaa taatcaatgg
     3841 aaataaagac aagagtaaat aataagcaac gacgacagag gtcgtactca tttctgaatc
     3901 agtgatgcgc ttttgagtcg tctcaataaa gcgcatcaga ttttcatttt gttttatttt
     3961 gttccaaact ttcatgtacc tctcccgtac ctattgactg gatagatcaa taagtgcctt
     4021 cttctccttg tgatgtcaaa attcttggac catctttagt aatagccaat gtatgttcat
     4081 attggcaaga aagtccgccg tctcttgtat acgcagtcca gccgttagga tccattttca
     4141 ttttccaagt tccggtatta accataggtt cgatcgtgat aaccatgcct tctttcaacc
     4201 gcaacccttt tccagcttct ccgtaatgag gaactgctgg ttcttcatga atcgtaggac
     4261 caacgccatg accaataaaa tcacgaacga tcgctagatt ttcgccttct acataagttt
     4321 ggatagcatg accgatgtca ccaatccgat tccctacttg tgcttgctca ataccgatgt
     4381 acaaagcttt cttcgttact tccatcaatc ggtcgatctc aggtgtagat tctccaacta
     4441 cataggccca acaagaatca gaaatgccgc ctttcaaatc gatacacata tcgactttta
     4501 ccaaatcacc gtttttcaaa ggctttttac gagggaatcc atgacaaatt tcatcattta
     4561 tactgataca agtcgcatat tcataccctt caaagccaat ttgcgcagca attccgccat
     4621 gactctcgat atagtttctg acaaatacct caatatccca acttgtgatt cctggtttta
     4681 taaaatcacg cagattttta tgtacatctg ctaataaagc tccagattct gccatcattt
     4741 caatttcacg tgctgatttt aaagtaatca tattttcgtc tcctttttac aaatccctct
     4801 cattttaaca tagcggcata catttagcca aatgacattc cccaggtgtc tctaggtttt
     4861 attttctttt caatgatgtt ttggtataat tcataattgt taaataatta taaacgagga
     4921 ggcacatcat ggctttagca aaaatcgtat atgctagcat gacaggaaac actgaagaaa
     4981 tcgccgatat tgttgcagaa gcatttgaaa atctggatat cgaagtcgaa atcaacgaat
     5041 gtacccaagt agatgcagat gaattcgagg aggcagatat ttgcgtagta gctacttata
     5101 catacgatga tggcgatctg cctgatgaga tcgtagactt ttatgaagac ttgcaagaat
     5161 tggatctatt gggaaaaatt tacggtgtct gcggatctgg tgatactttt tacgatgaat
     5221 tctgcaaatc ggtggatgat ttcgatgctg cttttgcaaa aacaggcgca tcaaaaggag
     5281 ccgaaaatgt taaagtggat ctgaatgctg aagaagaaga tatcgaaaat ttagaagctt
     5341 tcgctaaaga actagtcgca aaaatttaat atacttgcaa taaaagagcc atccagattt
     5401 attcaatctg tatggctctt ttattcattg cattcatgga agagttcttc gggagttaaa
     5461 aatgcatacg ctgtaagaaa aacgaacttt tccttatttt gacacatctc ttgttaccta
     5521 ctgatttgtg gaacttttga gtatattcaa tcgatttcga ttggttcttt gaatttcaag
     5581 atagcatcta tactgtcttg gatcatcttt tcaatctgtt catcagttgg atggatgatg
     5641 cttgaagaca tcaaagctgc gatcccagta gctatgatcc atgtcccatt atacaaagat
     5701 tcgatctgct cttctgaaag attcacataa gctggatcgc gtttcactgt attcacaaag
     5761 taagtataag agaaattctg catctctttt cctccaccat attcttctaa gtacagtgaa
     5821 cgatacaact tcttctctct tcttgcaaaa tggatataat taaaagcaag atcgataatc
     5881 gtattccctg tatgctcaac agtgaacact tcatattgta aatgatcata gatctgctca
     5941 aaaagagctt gtctcaagtc atccatattt ttaaactcaa gataaatcgg ttgcgtggaa
     6001 caattcattt tgttcgcgat gtttctcgca gtaaatcgtg taaatccatc ggtggcagct
     6061 acttcataag cagctgccaa aatctgttct ctagtaatcg tcttttttct tgccatattt
     6121 tttaatctcc ttaatttttt tataacgctt ccaccattga ttaatttcat tgt
//
LOCUS       DANJMI010000187         4660 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.guided.115, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000187 DANJMI010000000
VERSION     DANJMI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.guided.115"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            152..958
                     /locus_tag="PWU81_003155"
     CDS             152..958
                     /locus_tag="PWU81_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HDL1111756.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            976..1683
                     /locus_tag="PWU81_003156"
     CDS             976..1683
                     /locus_tag="PWU81_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDL1111757.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            1680..2651
                     /locus_tag="PWU81_003157"
     CDS             1680..2651
                     /locus_tag="PWU81_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HDL1111758.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            2644..3672
                     /gene="vanB"
                     /locus_tag="PWU81_003158"
     CDS             2644..3672
                     /gene="vanB"
                     /locus_tag="PWU81_003158"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HDL1111759.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            3678..4286
                     /locus_tag="PWU81_003159"
     CDS             3678..4286
                     /locus_tag="PWU81_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HDL1111760.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(4352..4525)
                     /locus_tag="PWU81_003160"
     CDS             complement(4352..4525)
                     /locus_tag="PWU81_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111761.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1314 a          980 c         1237 g         1129 t
ORIGIN      
        1 ttttgaccga caggtataac cctgccggtc tttttttgct gctacaggaa aactacagac
       61 tggctacagg gaaagtacag atgcgcttgc cataataaca atcgtaccag ccacaaatcg
      121 tagttttatt gcaaaggagg cattcgatca aatggaaaaa agcaactatc attccaatgc
      181 ggatcatcac aaacggcata tgaaacaatc cgtggaaaag cgggcttttc tatgtgcgtt
      241 cattatttcg ttcacagtct gcacgctgtt tttggggtgg agactggctt ccgtattgga
      301 ggcaacacag ataccgccca tccctgcaac tcatacaggc agcagcactg acgtagtgga
      361 gaatttggag gaaaacgctc ttgccaccgc caaagaacag ggagatgaac aggaatggag
      421 cctgatttta gtgaacaggc agaaccccat ccccgcacag tacgatgtgg agcttgagca
      481 actatcaaat ggtgagcgga tagatattcg gatttctccc tatcttcaag atttgtttga
      541 tgccgcaaga actgatggag tttacccgat tgtcgcatcc ggataccgaa caacagaaaa
      601 acagcaagaa attatggatg aaaaaattgc cgaatataag gcgaaaggct acacctctgc
      661 acaggctaaa gcggaagcag aaacttgggt ggccgtgccg ggaacgagcg agcatcagct
      721 tggtcttgct gtggatatca atgcggacgg aattcattca acaggcaacg aggtttatag
      781 atggctggat gaaaacagct atcgttttgg ttttattcgt cgctacccac cagacaagac
      841 agagataacc ggtgtgagca acgagccgtg gcattaccga tatgttggca tcgaagctgc
      901 cacagagatg tacaaccaag gggtttgcct tgaggaatat ttaaaaccag aaaaatgaga
      961 aaaggatgtt atgttatgga cagaaaaaga ttgacacagc gtttcccgtt cctgcttcca
     1021 atgagacggg cgcagagaaa aatgtgcttt tatgcgggaa tgagatttga cggctgtcgc
     1081 tatgcgcaga cgatagggga aaaatcgctt tcccatttgc tctttgaaac ggattgcgca
     1141 ttatacaacc acaataccgg atttgacatg atataccagg aaaacaaggt gttcaactta
     1201 aagctggcag caaagacctt aaacggctta ttgataagac cgggggaaac cttttctttc
     1261 tggttgttcc tgcatacgcc attgacaatt atccagcgca gaggtcacga agtaaaggag
     1321 tttccagagc caaacagtga cgagattaaa ggggtggatg caaccatttc agagggctgg
     1381 attgatttaa aagtgcgaaa cgataccgac tgcacctacc aaatatgggt gaccttagat
     1441 gatgagaaaa tcatcggtca ggtgttcgcc gacaaagagc cccaagcatt atacaaaatt
     1501 acaaatggca gtatccagta tgtccgtgaa agtggcggga tttatgaata tgcccaggtt
     1561 aaacggatgc aagttgcctt aggtaccggg gaaataatag attgcaagct gctttataca
     1621 aacaaatgca aaatctgtta tcccctcccg gaaagtgtgg atattcagga ggagaaccaa
     1681 tgagaaaaag tgtgggcatt actgtttttg gatgcgaaca ggatgaggca aatgctttcc
     1741 gcgctttatc gccggatttt catattatcc ctacgctgat tagcgatgcg atatcggcag
     1801 acaacgcaaa attggccgct ggcaatcaat gcgttagcgt aagccataag tccgaggttt
     1861 ccgaggcgac aattcttgcg ctgagaaagg tcggggtaaa atacatttct acccgcagca
     1921 tcggctgcga tcacattgat acgactgccg ccgagagaat gggaatctcg gttggcacgg
     1981 ttgcgtattc gccggacagc gttgcggatt atgctttgat gctgatgctg atggccatac
     2041 ggggtgcaaa acccaccatg cacgccgtgg cgcaacaaga tttcagattg gatcgtatcc
     2101 gggggaaaga actgggggat atgactgtgg gagttattgg aaccggccat atcgggcaag
     2161 cggtcgtcaa aaggctgcgg ggatttggat gccatgtgct ggcctatgat aacagccgaa
     2221 aaatggatgc agattatgtc cagcttgatg agcttctaaa aaacagcgat attgttacgc
     2281 tccatgtgcc gctttgtgcg gatacccgcc atctgatcgg tcagaagcaa attggagaga
     2341 tgaagcaagg cgcattttta atcaacaccg ggcgcggagc gcttgtcgat accggggcgc
     2401 tggtggaggc gttggaaagc ggaaagctgg gtggtgcggc actggatgtg ttggagggcg
     2461 aggatcagtt tgtttatacc gattgctcgc agaaagtgct tgaccatccc tttttgtcgc
     2521 agcttttaag gatgccgaat gtgatcatca caccccatac ggcgtactac actgagcgtg
     2581 tgctgcagga tactacagaa aaaacaatca ggaattgtct caattttgaa aggagtttac
     2641 agcatgaata gaataaaagt cgcaatcatc ttcggcggtt gctcggagga acatgatgtg
     2701 tcggtaaaag ccgcaataga aattgctgcg aacattgata cggaaaaatt cgatccgcac
     2761 tacatcggaa ttacaaaaaa cggtgtatgg aagctatgca agaagccatg tacggaatgg
     2821 gaagccgaca gtctccccgc catactctcc ccggatagga aaacgcatgg gctgcttgtc
     2881 atgaaagaaa gcgaatacga aacacggcgt attgatgtgg ctttcccggt tttgcatggc
     2941 aaatgcgggg aggatggtgc gatacagggg ctgtttgtat tgtctggtat cccctatgtg
     3001 ggctgtgata ttcaaagctc cgcagcttgc atggacaaat cactggccta cattcttaca
     3061 aaaaatgcgg gcatcgccgt tcccgaattt caaatgattg ataaaggtga caagccggag
     3121 gcgggtgcgc ttacctaccc tgtctttgtg aagccggcac ggtcaggttc gtcctttggc
     3181 gtaaccaaag taaacggtac ggaagaactt aacgctgcga tagaagcggc aggacaatat
     3241 gatggaaaaa tcttaattga gcaagcgatt tcgggctgtg aggtcgggtg tgcggtcatg
     3301 gggaacgagg atgatttgat tgtcggcgaa gtggatcaaa tccggctgag ccacggtatc
     3361 ttccgcatcc atcaggaaaa cgagccggaa aaaggctcag aaaatgcgat gattacagtt
     3421 cccgcagaca ttccggtcga ggaacgaaat cgggtgcagg aaacggcaaa gaaagtatat
     3481 cgggtgcttg gatgcagagg gcttgcccgt gttgatcttt ttttgcagga ggatggcggc
     3541 atcgttctaa atgaggtcaa taccctgccc ggttttacat cgtacagccg ctacccacgt
     3601 atgatggccg ccgcaggaat cacgcttcct gcactgattg acagcctgat tacattggcg
     3661 ttaaagaggt gataagtatg gaaaatggtt ttttgttttt agatgaaatg ttgcatggcg
     3721 tccgttggga tgccaagtac gccacatggg ataacttcac aggaaaaccg gtggatgggt
     3781 atgaggtgaa tcgcatcatc ggcacaaagg ccgtggcgtt tgctctgcgc gaggcacaaa
     3841 tccatgcggc tgcgcttggc tatggcttgc ttttatggga tggatatcgg ccaagaactg
     3901 cggtggactg cttcctgcgt tgggcagcgc aaccggagga caatctcaca aaagaaaaat
     3961 tttaccccaa tatagagcga gccgagttga ttacaaaggg ttatgtggcc tcacaatcca
     4021 gccatagccg tggaagcgca attgatctta cgctctacca cctggataca ggggaacttg
     4081 tttcaatggg aagtaacttc gattttatgg acgaacggtc gcatcataca gcaaaaggga
     4141 taggggatgc agaggcacaa aatcgaagat gcttgcgtaa aatcatggaa agcagcggat
     4201 ttcagtctta tcgctttgaa tggtggcact ataagttgat tgatgagcca taccccgata
     4261 cctattttaa ttttgccgtt tcataatgaa agtattggtt ttgtatcaga ttttggattt
     4321 gataaaatct atgctggtca tggacaatct tttactggaa agtggttaaa gcatcttttg
     4381 taatacattg caaattatag tattcaatct gcccagcggc agaaaagaaa aaaaccgctg
     4441 ttgggcagac ctttcccacg cccattttgt attttgctgg cggcggtttt gagaaatcaa
     4501 ggccgccgtt cttttctggc agcaaaaggt gtggcggccc ataggaggat gccgctatgc
     4561 tgttttgcta ttgccatgct ctaatccact tccaccactc aaaaaaagcc ttgcccctcc
     4621 atgaactgaa ccccaaaagt tgaactattt aatggactgt
//
LOCUS       DANJMI010000188         4454 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.guided.119, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000188 DANJMI010000000
VERSION     DANJMI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.guided.119"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            175..672
                     /gene="dfrG"
                     /locus_tag="PWU81_003161"
     CDS             175..672
                     /gene="dfrG"
                     /locus_tag="PWU81_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL1111762.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(744..2696)
                     /locus_tag="PWU81_003162"
     CDS             complement(744..2696)
                     /locus_tag="PWU81_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL1111763.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2703..2939)
                     /locus_tag="PWU81_003163"
     CDS             complement(2703..2939)
                     /locus_tag="PWU81_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111764.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            3341..3631
                     /locus_tag="PWU81_003164"
     CDS             3341..3631
                     /locus_tag="PWU81_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL1111765.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT         1421 a          737 c          779 g         1517 t
ORIGIN      
        1 ataaaatgca attgaaaatg ataattgaat gaaaatttgt gattttgtgg tattatgata
       61 atataactta tagaaaaaat tgcttattca actaacgggc aggataatga gaaaaggttt
      121 tttgatagaa ataagaaatt agatggtaaa ttagttaaaa aggctgtgtt ataaatgaaa
      181 gtttctttga ttgctgcgat ggataagaat agagtgattg gcaaagagaa tgacattcct
      241 tggaggattc ccaaggactg ggaatatgtt aaaaatacta caaagggaca tccgataata
      301 ttaggtagga agaaccttga atcaatcgga agagccttac ctgacagaag aaatattatt
      361 ctgacgagag ataaggggtt tacctttaat ggttgtgaaa ttgttcattc aatagaagat
      421 gtttttgagt tatgtaaaaa cgaagaagaa atttttattt tcggaggaga acagatttat
      481 aatttgtttt tcccttatgt tgagaaaatg tacatcacaa aaatacatca tgaattcgaa
      541 ggagatactt tttttccaga agtgaattat gaggaatgga atgaggtatt tgcccaaaaa
      601 gggataaaga atgataaaaa tccgtataac tactattttc atgtatatga aagaaaaaac
      661 ttattgagtt aacgggtgcg ttaattgaag aagaacagta aagataaagc acaagagctt
      721 gggattactc caagctcttt ttgtcatttg aaataaatta tatctttcca atcaaattgt
      781 ttatctgtaa tccctaatct ttgagcaggt gtcaaaacct ttttatccgc tgatttaaaa
      841 gggcgacaaa agttgtagta agttcttaga attgtaatcg caaattgagc atacttcgga
      901 ttgaagttag cgtaaatata gctcttctta tcaccacgag ctgttgttaa tggtctttca
      961 agagatgata tataacgtct tatctgttga ataaagctat tggtcgaatg gtcattaaca
     1021 tttaatagca tctttgcgat ttcgtttggt tccagagcag ataaatccgt tctacaatca
     1081 actgaataaa atcctttatc cttagtggct aatggatgtt tgatagggtt atcggcatat
     1141 ttgcgatatc tttctccatc tttactaata gcctcttcgt ggaattgatg acttgttagt
     1201 aattcagtta gataatgaga tgccaaagtc cgtagaaact ttgttttgaa tcctctgata
     1261 tctccccaat cgagcagttc tgcttttgct tgtccgaatt cttttaagca ttgttttctg
     1321 cttttcgtct tgttaacttg gcaaataaaa tgatgtgcat cgctcaacct taattctttc
     1381 gtgaatactc tataaaaggc gtttctaata gagaagtcat catcactaat cattcgccat
     1441 tcgtcggcat taaccatttg tttaattaac caatagtgag cagttgtagt ataaggagca
     1501 tcaacattta aaccatcgac aaatctactc ctattagtaa ttttccctaa ttcgtgaaga
     1561 taagcattcc tattttcgct atcgttagcg gatggttctt gtggataata agaccaatct
     1621 aacctatcat ttttacgact aaaagtattg agatggtctt ctttgaattt tcttgtatct
     1681 tcatttaatt catccattga aatattccaa tcataggcaa catcagaacg gaacacatat
     1741 cttgataaga cctcagcaga tacaactata tacgtttgca tattaaggtc ttcaaacccc
     1801 gtatatttct ttgaaccttg tcctttctta cggacgttat ttaggtagta gtgcatcttg
     1861 tccgtattaa gccacatctc gttaaatttc tttgtttgaa ggggttgtgt ctcatatcgt
     1921 tctaaaaact ccaaacaacg cctatacagc cactccagct tatgatagta ggttccaact
     1981 ccaattccta aaatatcaca agtacgactg actgaaaccc ttccaacaac catcttagcg
     2041 aacattggaa gaatggtgtt cttttgttgg tgataagtag tggtttcttc tctttttggt
     2101 agaacattag tgaacttctt acacgcctta cattgatagc gttgagattt gcccctactt
     2161 ttcccccttt tgtaaaattg cttcggctca ttgaatgggg ttgattcttc ttccgagcag
     2221 ccttctttat gaaattgata atctggctca acatcttgga tgctattgat ttctataagg
     2281 cgtttaattt cttcaacaac agaccaatta gataaagggg ttactgtaca gttttgcgat
     2341 acacctctgc caataggatt gtcattgcag tacatacctt tatgaccttt atctttggaa
     2401 ctaccagtca ttttatatcg acttggtttc ccttttacat ctttgaattt gtgctgttct
     2461 ttcccaaaat tacaacaaaa cggattgatg cagtaattat attgaatggg atattcagaa
     2521 ccgttccatt taaaagacgt tggcaagaac aacattttgt tataccttgt tgcaaatcgt
     2581 tttggagtta ataaatcata ttgtttagcc ctatctttta attcttcgtt tgaaatcgga
     2641 attttaacat ctacaatttc atcttccttc gtggctaatc ttttaagctt ggtcattgtc
     2701 tatcaaccca ctctatcttt gatacccatt ttttcaacaa tccgcttatt acttcgcttg
     2761 ttggcaatcc attcaataaa cttcttatcg tctaaaatgt tcaataagaa ttcatccact
     2821 gcttcgcttt cagtacgctc tgcatattca gcgtattgct taactatcac ccttacttgc
     2881 tctgatattt cccaatccac agacttagcg ttcttatttt ttggctcaag aaattgcata
     2941 ggaaaatgca cccctttttg cttagtaaat tgtaaccatt ttaacacacc aatttgcatt
     3001 cctacaataa ttatttcacg ttcgcatttt tatatacatt ttaacttaca ttttgtggtg
     3061 taaattaaca acaaacgttg atttaacaat aaaaaacgag tatcaaattc agttgcaatt
     3121 tgatactcat ttaggtttac attttcactt tttgtactcc aaaactgaac tacttaaatc
     3181 tatactgctg cctctttttt ttagtacatg tccttcatct tcgcaggaag tgccaatatg
     3241 attttaagaa aggtaataga caaaaagtca aaaagataaa acagagtttc ttctttacaa
     3301 agggaatgta tgttcgtata atggtattga ggtgaaggtt atggagaaaa aatatggaac
     3361 agtagataag atccgtactt tgcaagttag tgccagaccg ttgatccgtt tcagtttaaa
     3421 tggggtcaat tgtttgattg cttcccatag tttgaacttc ctagctgaag tagacgaagg
     3481 gatgaagtta gtcgttacag gttattataa taatagaaag caatttgttg tgagatccta
     3541 tcatgtgtta gggaaaccca aaattgtggt agaatatgag aaaagtctat acccaagaaa
     3601 aaaggtccga caagatgaaa gaataaacta gaaaacaagc ggctgagaca aaaatgtcct
     3661 agccgcttgt tttggaaaga tggatgcaga cgatgggatt tgaacccaca cgagcatacg
     3721 ctcacaagaa cctcaatcta tatataacta catgtattta agtatttgtg atgaatcact
     3781 aaactttcca aactcatttg agggatattg cttaaagatt gaacagatca attcgaagcc
     3841 ctattagtat tcgctcaatg gggggatcag aggccataaa aagagactag tcattactag
     3901 tctcaggtag tggaattgta ggattttaaa cgttcctgga gagtaccggt atgtaactcg
     3961 aagaggtgat tatcatagtc gtaaaagtaa attgaagaag cctcgccttt cacacgagaa
     4021 cgtcctggtt cgacagttaa acctaacatt tggatttttg agacaaacga atctatatct
     4081 tgttcgtcaa ttttaaaagc aatatgattg tatgtctttg gcagtttatt cgacgaattc
     4141 tgcattactg caatccatag gtccttaact aagaaaaatt tttctggata taatgaaaat
     4201 ttttttgttg actatcgtag atttcttttg catcaaataa ggtatttaac aattaagtat
     4261 ttaaaagata acttttaagt tatctttaag taatgcttaa agctatgtta tactctatta
     4321 ggaaatataa gaaggagtat aaaaatgaac aaacataata gaaaaataac gaataacaaa
     4381 gaactgatct tgttactaat agggatttgg ggtatctttt tactctttaa tatgtatatg
     4441 ccgactatga gagc
//
LOCUS       DANJMI010000189         3796 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.guided.127, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000189 DANJMI010000000
VERSION     DANJMI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.guided.127"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            110..193
                     /locus_tag="PWU81_003165"
     CDS             110..193
                     /locus_tag="PWU81_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HDL1111766.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="PWU81_003166"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="PWU81_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL1111767.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="PWU81_003167"
     CDS             complement(1318..2181)
                     /locus_tag="PWU81_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL1111768.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="PWU81_003168"
     CDS             complement(2183..2455)
                     /locus_tag="PWU81_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL1111769.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="PWU81_003169"
     CDS             complement(2472..2681)
                     /locus_tag="PWU81_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDL1111770.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2759..3655)
                     /locus_tag="PWU81_003170"
     CDS             complement(2759..3655)
                     /locus_tag="PWU81_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL1111771.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1174 a          662 c          586 g         1374 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattct
     2761 attctttttc attttctaat tgaatgattc gctcaatcat ttcaaaaaat acgttctcat
     2821 acatagacaa aacttttttg tcatagcctt tgtgttctgt aatgccattt ttagaccaag
     2881 tacttacttt attacttcgc ttgataatat ttcggaaaac caaattatcc tctttatgtt
     2941 ctttgtacag ttcttccagg tttgatttta tcgttgcgct gtccgtatca actaaataag
     3001 gaacaaaacc aatcatatct agtccagggt taaactgttc ttgcaaatca atcaaatagg
     3061 aaatatagtt ttgaatgttg tttgtacttt cttcttctgc ttgtaaaggg atcataacgt
     3121 aatcactcgc cacgattgca ttatttgtat aaacgcttgg cgttggtaca gtatcaataa
     3181 taataagatc atagtcactt tttaaaggtg ctaaaagagt agcaagcaat ctactttcat
     3241 tttcaaacgt ccatgagcga gttaattttg gcagtaacat caaatcaaac gtgccaggga
     3301 tcaagtctaa attatcagtc aaatgaataa tagaagaagc caagtttcca tttttcaagc
     3361 cttcataaaa attgacacgt ggcaattcta cctcaaaagt ttttgctaag tcttttgtca
     3421 atgttgcctg taagtcctta tcgatcatta aaacttttaa attcaatttg tctgtcaagt
     3481 aagcaaacat agtcgataat ttggactttc caacaccacc tttaaagtaa ttattcaaga
     3541 taataattgc ttcattttta ttgtttaaaa tacgtcttaa ttcttcaagt atttttagtt
     3601 tttccttctc cataactacg ccccattctt tttttgtata gtaatattgt atcattaaga
     3661 atcacaaatc acaagtgatt tgtgattaat cacttgtgat ttgtgataag tgatttgtga
     3721 ttaatatata aaagccctct ttaaagggct tttataggtt ctgttgcaaa gttttaaata
     3781 aagaataaaa tccctt
//
LOCUS       DANJMI010000190          806 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538521-rid17231633.guided.171, whole genome shotgun
            sequence.
ACCESSION   DANJMI010000190 DANJMI010000000
VERSION     DANJMI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538521
            Sequence Read Archive: ERR10688084
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001637832.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001637832.1
            Genome Coverage       :: 93x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 16:34:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,031
            CDSs (with protein)               :: 3,031
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 31 of 78
            Pseudo Genes (internal stop)      :: 23 of 78
            Pseudo Genes (multiple problems)  :: 5 of 78
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538521-rid17231633.guided.171"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2020-12-16"
     gene            193..741
                     /gene="aac(6')"
                     /locus_tag="PWU81_003171"
     CDS             193..741
                     /gene="aac(6')"
                     /locus_tag="PWU81_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL1111772.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          285 a          121 c          174 g          226 t
ORIGIN      
        1 ttttcctcca attgatggct ttcctgatct ttcctatttt gaataaagtg tttctgtata
       61 attttttatt ttataagtaa gataaataat gaaagaatag agatactgat tggtagctaa
      121 agatttttga gcgagaacat ttcgtttttt caaaaggact gttagaataa gcaagaacaa
      181 gggggaagac gtatgataat cagtgaattt gaccgtaata atccagtatt gaaagatcag
      241 ctttctgatt tactgagact gacttggccg gaagaatatg gagacagctc ggcagaagaa
      301 gtagaagaaa tgatgaatcc agaacgaatc gcggtagcag cggtagacca agatgagtta
      361 gtaggattta ttggtgcaat ccctcaatac ggtatcacag gttgggaatt gcatccatta
      421 gttgtagaaa gctcccgacg aaagaaccaa ataggtactc gattagtcaa ttacttagaa
      481 aaagaagtag cttccagagg aggaatcacg atttatttag gtacggatga tttagaccat
      541 ggaacaacgt taagtcaaac ggacctgtat gagcatacat ttgataaagt ggcttccatc
      601 cagaaccttc gtgaacatcc gtatgaattc tatgaaaaat taggttataa aatcgtaggt
      661 gtcttaccaa atgcaaatgg ctgggacaaa ccggatattt ggatggcaaa aacgattatt
      721 cctcgaccaa attctcaata ataaaaagag gttgtgacag aagtaatcag cttcaagaaa
      781 taagaaggaa tttacgaaaa ttactt
//
